LOCUS       DAOQVJ010000001        11456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_6361_length_11456_cov_4.158846, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000001 DAOQVJ010000000
VERSION     DAOQVJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11456
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6361_length_11456_cov_4.158846"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..964
                     /locus_tag="PKL26_00005"
     CDS             77..964
                     /locus_tag="PKL26_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS82110.1"
                     /translation="MIEARNLTKCYGTTPALDHISFDLDRGQIFGVIGHNGAGKTTLL
                     KILAGLVEPTSGELVMDGVDILRSPDGMKQMIGYLPEESRLYETMRVDEYLSFFGEIY
                     GMDRSSIRARSRELLEWLALEPNGKKMGEFSKGMKRKAAIARSLMHDPSLLIYDEATS
                     GLDPMTSRHIVDFLRDLRDHGKTIVLSAHNLYQVEAICDRILILRRGKRVALGTMQEL
                     REMFGSIFYFVYFTVGDIGKVEGLSSYVMDGGYFRAKATSVEEMGRFSNAIVNAGGAV
                     ERIESHYPSLEEMLVKIGA"
     gene            complement(1109..1936)
                     /locus_tag="PKL26_00010"
     CDS             complement(1109..1936)
                     /locus_tag="PKL26_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82111.1"
                     /translation="MNRCSDTHWTRRNTTLRAVIVLICGFSLLLAPAAAAPITASIST
                     SPSDNYQMKASGYEQEGMALMAVRDWNGLINLTSEGLSTYPDEAELMCLKAYALRKTG
                     HFQEAVDLLNVAIPLDPRPARYANRGYAFLAMGRTNEALEDAEQAILLNSSYSSGQGL
                     KAETLLAMGDFPAALASADAALALEPENAHYWHVRGNALAGLGDCSGAFVSLEYSISL
                     KNEYDLPWPGLPNASTDLARVRSACMMPQETPAPTQAALPALMVVAALVVAVAEKRK"
     gene            complement(2046..2119)
                     /locus_tag="PKL26_00015"
     tRNA            complement(2046..2119)
                     /locus_tag="PKL26_00015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2083..2085),aa:Lys,seq:ctt)
     gene            2268..3731
                     /locus_tag="PKL26_00020"
     CDS             2268..3731
                     /locus_tag="PKL26_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS82112.1"
                     /translation="MPEAGGHVAGEKVKYAVLGCGSTGYHVATELSRKEGRVLVLDRD
                     EGRVKELRDQNVDAMIRDISDPGFLSGLPMFEIAFVLTGDHHSNLAAVRTLRQKLPLV
                     HIVARALDPIGAGTLESAGADFVLYPQQVVARAAIHQINRLHVSRLSQRLHDLLAGWE
                     GTLGIITHKNPDPDAIGSAMALAAIARNANPQKLACRIFYDGIIGHQENRTMVNLLDI
                     KMEKMEPQTIQDCTYLALVDCPAPGMNNTLSPRTRVHIVIDHHQDGHGLEGAMFVDVR
                     PGIGATASILSQYLQELDIPVDKNVATGLFYGIRSDTRDFRRNVTPQDLSNAAFLLPL
                     TDSDLLDQIMSPSLSQETLDVLGNAIRNRRIRSGYLFANVGFVRNRDSLPQAADLLIS
                     LEGVNTALVYGISDDAIIISSRNRDIRLHIGNVLEEAFGDIGDAGGHPNMAAATIPLS
                     YFRRVKNKEELLGLIMDPLLKKFTSLVGLDAEAGNEL"
     gene            3721..4335
                     /locus_tag="PKL26_00025"
     CDS             3721..4335
                     /locus_tag="PKL26_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNS82113.1"
                     /translation="MNFDEWEPIYEEIVEYFGFDRKEDEYAADILEELLERDDLDLLE
                     ETCRGHDVCVCGNAPCLAKEIPLVRGTVLAADAASLVLLSHGIRPDVIFTDLDGATDE
                     FLDMNREGTIVVVHAHGDNIPLVRYWVPRFPGPLVGTTQSRPLPHVHNFGGFTDGDRA
                     VFAAHSLGAAGITLLGFDLEDTRVDPMKRGKLYWAGRLLSLIEP"
     gene            4332..6005
                     /locus_tag="PKL26_00030"
     CDS             4332..6005
                     /locus_tag="PKL26_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS82114.1"
                     /translation="MTGKSAFILDGYVDEPACLGVPPYISPYVRTVAGTLFAHGYSAG
                     YLTIDQVRKDPALIHRMHAADLVVMIAGVTVPGKYLGGTPATLTEIRLIGTQIRGPEK
                     LLAGPIGFGYSSGGGQRAVKDAVSGFGHLLTGSPASALDSFLREGKTHGSFNYRREDP
                     WAVAGAAIIRDHPSFPWVMCEIETARGCSRAHSGGCSFCTEPFYGPPAFRSIDGVAAE
                     IDALHRAGGLHFRLGRQPDILVYGSSTAEEYPTPVPGRLEELFSAVREAAPGLLTLHI
                     DNANPGTIARHPEESRAALDVIVAYHTPGDVAAFGMETADPAVIEENNLKAGPDQVMD
                     AIRIVNEAGGRREDGIPELLPGLNFIAGLAGETPLTFDLDREFLAAVLASGYLVRRVN
                     IRQLMPFEGTRAFSENALGVHKGLFAKFKDLVRKEFDLPMLAKVFPTGTILRSVVMEE
                     EGATSFGRQMGSYPILVGIPLRVPQRTLLDTVVVDHGMRSLTGLPVPIEVNLLPVATL
                     AWVPGVGRKRAGQIAAKRPFSNLAEFRAVAGPTPVDPCLSFSFKSPSAR"
     gene            complement(5983..7115)
                     /locus_tag="PKL26_00035"
                     /pseudo
     CDS             complement(5983..7115)
                     /locus_tag="PKL26_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            7165..7437
                     /locus_tag="PKL26_00040"
     CDS             7165..7437
                     /locus_tag="PKL26_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS82115.1"
                     /translation="MRTQITELFGLNIYTEKSVFVGEVEDVILDIEGKKMESLVVGKL
                     NQDLVDIKNYKGLKLPFRIIRSIGDIVIIRHLPGAFKSGDFGEAGK"
     gene            7494..8225
                     /locus_tag="PKL26_00045"
     CDS             7494..8225
                     /locus_tag="PKL26_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HNS82116.1"
                     /translation="MKHRELFSNLVALPPIFLRLDGRTFHRLAETWCLERPFDSHFAD
                     GMARVSARLISESGLSPDLAFTFSDEISLYFSRLPFNGRVEKLDSVCASFAASALAIE
                     MESTVPVSFDARIIHVIPEMAPDYLIQRQGEAWRNHLNAYCQYALMMDGMSRKEAASF
                     LKEKPSQEMHELAFARGINLAHTPTWQRRGILVQKKTYMVKGFNPKLGKMAESIRSEV
                     VTSRDLPVFASPEGRALLSSLLRCP"
     gene            complement(8205..11390)
                     /gene="ileS"
                     /locus_tag="PKL26_00050"
     CDS             complement(8205..11390)
                     /gene="ileS"
                     /locus_tag="PKL26_00050"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNS82117.1"
                     /translation="MKEVSSSYQAQEIEESVQKYWREENTYGKVKELHADGKDFFFVD
                     GPPYTTGHIHLGTAWNKIIKDCMLRYHRMCGRNVIARAGYDMHGLPIEVRVEQTLGFS
                     SKKDIERFGIAAFIDRCRTFADTNKDIMSEQFMHLGVWLDFDDPYQTMHPGYIEAAWW
                     TLKRADEEGMLERGFRVVNWCPRCATAIADAEVEYWDERDPSIFVKFPVHGSEGEYLV
                     IWTTTPWTLPANVAVAVHPEFTYARVEAVKEGKKEVLWIAEDLVEPVLRKGRYQDYKV
                     IETCSGREMCARTYHSPLRDQVPLQAEISHRVVTADFVAMENTGMVHIAPGHGWDDYL
                     LGCKEDLPIICPVDAQGHFTGDAGVLSAMFIKDADQVVLRELGEHLLASDHVVHRYGH
                     CWRCKTPIIFRATSQWFLKASLLKDRMLEEVGKVTWYPDWAGSARFYDWIKEARDWCI
                     SRQRFWGIPIPIWTCESCGTHRVIGTIAELEDASGSPVPDPHRPWVDGVEIPCTCGGT
                     MRRVEDIFDVWFDSAVASWATLGFPREKEALKRLWPADFITEGQDQTRGWFYSQLGAS
                     TIAFGRAPYRSVLMHGFALDAEGRKMSKSLGNVVTPEEVVQSQGVDVLRLYVLSQNAP
                     WDDLRFNWDGVKNVSRAMNILWNVYRFPLPYMIIDNFSPRSGGRTWDDGFVRKEFPSL
                     WPEDRWIVSRVNSLARQVTADLSECQLHRASRTIIAFILEDLSRWYVQLVRPRMWLEG
                     EAPEKTRAYETMYYTLRTLLAIASPFVPHITEAMYRNLRLEGDPESVHMLDWCAGNDT
                     LRDEPLERAMDIVRSFDEAQANARQAGKRKLRWPVRECVIVADSQEVADAVSSLNHIC
                     RDRANARVVRAVSGHWERIGWRAEPVMKALGPAFGKQAPRVRDLILNADGNAMKQAID
                     SGNAFILENGGERFEIVAGQVTFAQQLPDGIFSAPMLGGTVYVDVTLDPDLEAEGYAR
                     EIIRRFQEMRRQLDLKVEDFVTADACITDSRICGMVAESWKTGIMEEIRATSFGVQGG
                     DCPAATSHEMSKEWDVEGIAMTLSLSRAPEQ"
BASE COUNT         2391 a         3426 c         3287 g         2352 t
ORIGIN      
        1 tgcctctttt atggtaggtt cagtacctat aataccttgc aggtcgagga attgtaaagg
       61 attggacaag gaatccatga tcgaagcccg caatctcacc aagtgctacg gcaccacccc
      121 tgcactcgat cacatcagct tcgaccttga tcgcgggcag atattcgggg tcataggaca
      181 caacggggcg ggaaagacca ctcttctcaa gatccttgcg ggactcgtcg agcccaccag
      241 cggggaactt gtcatggatg gagtcgatat ccttcgcagt cccgacggga tgaaacagat
      301 gatcggctac ctccccgaag agtcacgcct gtacgagacg atgagggtgg acgaatacct
      361 ctcattcttc ggggagatct acggcatgga ccggtcgtca atacgcgcac gcagccgcga
      421 actcctggaa tggcttgcgc tcgagccgaa cggaaagaag atgggggagt tttcgaaggg
      481 gatgaagcgg aaggccgcga tcgcccgatc cctgatgcac gatccatccc tccttatcta
      541 cgacgaggcg acttcagggc ttgaccccat gacctcccgg cacattgttg acttcctcag
      601 ggacctccgg gaccatggca agacgatcgt cctctcggcc cataacctct accaggtaga
      661 ggcgatatgc gacaggatcc tcatcctccg gcggggaaag cgtgtcgcac tcggcaccat
      721 gcaggagctg cgggagatgt tcggctcgat cttctacttt gtgtacttca ctgtcgggga
      781 catcggaaag gtcgagggcc tctcatcgta tgtcatggat ggggggtatt tccgggcaaa
      841 ggcgacgagc gtcgaggaga tgggaaggtt ctcgaacgcg atcgtgaacg ccggcggagc
      901 ggtggaacgg atcgagtccc actacccgag cctcgaagaa atgctggtga agataggagc
      961 ataaaaccgg cagatctctt tgacctggcc caatttgttt tcttcatctt tgaatgtcac
     1021 agatactcga gatatgtttc aggaagcggg cattccaacc cacatctcct cttcagaatc
     1081 cggagacagg tttgtaattg cacgttccct attttcgctt ttctgcaacc gcgaccacca
     1141 acgcagcaac caccatcagg gcgggaagtg ccgcttgtgt cggggcgggt gtttcctgtg
     1201 gcatcataca ggcagaacgc acccgtgcga ggtccgtgct cgcatttgga agcccgggcc
     1261 acggtaaatc atactcgttt ttcaatgaaa tggaatattc gagcgataca aaagccccgc
     1321 tgcagtcacc cagaccggcg agcgcgtttc cacgtacgtg ccagtaatga gcgttttccg
     1381 gctcaagggc gagggcggca tcggctgatg caagtgcagc aggaaagtcc cccattgcaa
     1441 ggagagtttc tgctttcaat ccttggcccg aggaataaga agaattgagc aggatggcct
     1501 gttctgcgtc ctccagtgct tcattagtcc ttcccatcgc gaggaacgcg tatcccctgt
     1561 ttgcataccg tgccggcctt gggtcaagcg gtatcgccac gttgagaagg tccacggctt
     1621 cctggaaatg tccggtcttc ctgagggcat acgccttcag gcacatcagt tcggcctcat
     1681 caggataggt tgaaagaccc tcgctggtga gattgataag tccgttccag tcacgcacgg
     1741 ccatcagcgc catgccctcc tgctcgtaac ctgaagcctt catttgataa ttgtctgacg
     1801 ggctcgttga gatggatgcg gtgatggggg cagcggctgc cggtgccagc aggagagaaa
     1861 aaccgcagat taagacaata acagcccgga gcgtggtatt ccgccgggtc caatgagtat
     1921 ctgaacaacg gttcatatca tttcagggga ttgggtatct tatagagatt tgtacatgat
     1981 atctcatgga atggcttggc caaaaatcaa aacactgcat gaacagatac aatagaaaaa
     2041 gaaagcgggc ctgaagggat ttgaaccctt gacctacgga ttaagagtcc gtcgctcttc
     2101 cgaactaagc tacaagccca tgcctgatcg gcctatattc atggttccga attgagtata
     2161 aatgttttta tttttctgcc agtaaccccc ccatgcggca gtttccaacg cgcaatctcc
     2221 ccgtttcagg ttgtaacctt aatatcatca aggagtgaca ttgtagcatg ccagaggcgg
     2281 gcggacatgt ggcaggagag aaggtgaaat atgcggtcct cggatgcggg agcacggggt
     2341 accacgtggc cacggaactg tcgcgaaaag aaggccgggt ccttgtgctg gatcgcgacg
     2401 agggacgggt aaaggagctc cgggaccaga acgttgacgc catgatacgg gatatctctg
     2461 accctgggtt cctctccggg ctccccatgt tcgagatcgc atttgtcctc accggggacc
     2521 accactcgaa cctcgctgca gtcaggacgc tccgccagaa actgccgctt gtgcacatcg
     2581 tggcccgtgc gctcgaccca atcggtgcag gaacgctcga atcagcaggg gcagacttcg
     2641 tcctctatcc gcagcaggtg gtagccaggg ccgcaatcca ccaaatcaac cggctccatg
     2701 tcagccggct ctcgcagcgc ctccatgatc tccttgcggg gtgggagggg actcttggaa
     2761 tcatcacgca caagaatccc gacccggacg cgatcggaag cgcaatggcc cttgcagcca
     2821 tcgcccggaa tgcgaatccc cagaagcttg cctgccggat cttctatgat gggatcatcg
     2881 ggcaccagga gaaccgcaca atggtcaacc tcctcgatat caagatggag aagatggagc
     2941 cccagaccat tcaggactgc acctaccttg ccctcgtcga ctgccccgcg cccgggatga
     3001 acaacaccct ctcgccaagg acgcgggtgc atatcgtgat cgaccaccac caggacggcc
     3061 atggcctcga gggggcgatg ttcgtcgacg tgaggccggg gatcggcgcc accgcaagca
     3121 tcctctccca gtacctccag gaactcgata tcccggtcga caagaatgtt gccaccggcc
     3181 ttttctatgg aatacgttcc gatacgaggg acttccgccg gaacgtcacc ccacaggacc
     3241 tctcgaacgc cgcgtttctc ctcccgctca ctgactctga cctgctcgac cagatcatgt
     3301 ctccttctct ttcgcaggag acccttgacg tgctcggcaa tgccatccgg aaccggcgca
     3361 tcaggagcgg gtatctcttt gcgaatgtag gatttgtacg gaaccgcgac tccctgcccc
     3421 aggcagcaga tctcctgatc tcccttgagg gggtgaacac cgcgctcgtg tatggaatat
     3481 ccgatgatgc catcatcatc tcgtcacgga accgggatat acggcttcac atcgggaatg
     3541 tcctcgaaga ggcatttggg gatatcggcg atgccggggg ccaccccaat atggccgccg
     3601 caaccatccc cctctcctat ttccggcggg tgaagaacaa ggaagaactc ctcggcctga
     3661 tcatggaccc cctcctcaag aagttcacca gcctcgtagg cctggacgca gaggccggaa
     3721 atgaactttg atgaatggga accgatttac gaagagatcg tcgagtattt cgggttcgac
     3781 cggaaagaag acgagtatgc ggccgatatc cttgaagagc tccttgagag agacgacctc
     3841 gacctgctcg aagaaacctg ccgggggcat gacgtctgcg tatgtggaaa tgcaccctgc
     3901 cttgcaaaag agatccccct cgttcggggg actgtccttg cggccgatgc cgcctctcta
     3961 gtccttcttt cccacgggat ccgccccgat gttatcttca ctgacctgga cggggcgaca
     4021 gacgaatttt tggatatgaa cagggaaggg acaatcgtgg tcgtgcacgc ccacggtgac
     4081 aacattcccc tcgtccgtta ctgggtgccg aggtttccag gcccgcttgt cgggaccacc
     4141 cagagccgcc cccttcccca cgtgcacaac ttcggcggtt tcaccgacgg agaccgtgcg
     4201 gtctttgccg cgcattccct gggtgccgca ggcatcaccc tcctcgggtt cgaccttgag
     4261 gacacccgcg tcgatcctat gaagaggggg aagctgtact gggccggaag gctcctctcc
     4321 ctgatcgaac catgactggg aagtccgcgt tcatcctgga cggctatgtc gatgaaccgg
     4381 cgtgcctcgg agttcctccc tatatctcgc cttatgtaag gaccgttgcg ggaaccctct
     4441 tcgcgcatgg atattcggca ggatacctga ccatcgacca ggtacggaag gaccccgccc
     4501 tcattcacag gatgcacgcc gcggacctgg tggtgatgat tgccggcgtg actgttcccg
     4561 ggaagtacct tggggggacg cccgccacgc tgactgagat ccggctgata ggtacacaga
     4621 tacgtggtcc cgaaaaactc ctggcgggcc ccatcggatt cgggtactcc tccggaggag
     4681 ggcagcgggc agtgaaagat gcggtctcgg gattcggcca cctcctcaca ggatctcccg
     4741 cctccgccct cgactcattc ctccgcgagg gaaagaccca cggttcattt aactaccgcc
     4801 gcgaggaccc atgggcggtt gccggtgccg caatcatcag ggatcatcct tccttcccgt
     4861 gggtcatgtg cgagatcgag acggcaaggg gatgttcccg ggcacactcg ggggggtgtt
     4921 ccttctgcac cgagccgttc tacgggccgc ccgcattccg ctcaatcgat ggggttgctg
     4981 ccgagatcga cgcccttcac agggccggcg ggttgcattt tcgcctcggg aggcagccgg
     5041 atatcctggt ctacgggtca tcgacggcag aagagtaccc gacgcccgtc cccgggcggt
     5101 tggaggaact cttctccgcg gtcagggaag cggcgccggg tctcctcacc ctccacatcg
     5161 ataatgccaa cccggggact attgcgcggc acccggagga gagcagggcg gctcttgacg
     5221 tgatcgtggc ctaccatacc ccgggggacg ttgcggcctt cgggatggag accgcggacc
     5281 cggcggtgat tgaagagaac aatttgaagg ccggtccgga ccaggtgatg gatgcgatcc
     5341 ggatcgtcaa cgaggctggc gggagacgcg aggacggaat ccccgagctc ctgccggggc
     5401 tcaacttcat cgcagggctt gccggggaga ccccgctgac ctttgacctt gacagggagt
     5461 tcctggcagc ggtgcttgca tcaggatacc ttgtccgcag ggtgaatatt cggcagctga
     5521 tgccatttga gggaacgcgg gcattctcgg agaacgcgct cggggtccac aaggggctgt
     5581 tcgcgaagtt caaggacctt gtcaggaaag agtttgacct tcccatgctc gcaaaggtgt
     5641 ttcccacagg gacgatcctt cgctcggtgg tgatggagga ggagggcgcc acgtcttttg
     5701 gccggcagat gggctcgtac ccgatactcg tggggatccc gctccgcgtc cctcaacgaa
     5761 ctctcctcga cacggtagtg gttgaccatg ggatgagatc cctgaccggg ctccccgtcc
     5821 cgattgaggt gaacctgttg ccggttgcaa cgcttgcctg ggtccctggc gtggggagga
     5881 agagggcagg gcagatcgca gcaaagagac ccttctccaa ccttgcggag ttccgggctg
     5941 tcgccgggcc gacccctgtc gatccctgcc tctccttctc ttttaaatct ccttcagctc
     6001 gatgaccttc aggatatctg tccgggtgat gatgccgacc agatggtcat gttccatcac
     6061 ggggatccgg ccgatattat agcgggacat cagccgcagc ccttcgatca caggcgaccc
     6121 gggggccagt gcaatcacct cgcgtgtcat gatgtccctt gcctgcatcg cttccctgtc
     6181 caggggaggg accttgtgca cgtcggccag ggtgatcatc cccacgacga acccacgatc
     6241 cacaaccgga aacccgaggt gcttggtgga gtacatgaga tcgatcacct ccctgaccgg
     6301 catggtcggc gggacggtca tcacctgccg ttccataata tcgcccacat tcacgtcttt
     6361 tagcaggaag ttgtacttaa tcgccgtaga ctcctgcccg gccccgatat agatgaagaa
     6421 cgcgatgagg ataaggatag gggagaaaaa aatgacgccg aatatcccga agatgaccgc
     6481 aaacatcttt ccgatgtttg ccgcgatacg ggttgcacgg tgaagcggca tcttccgggc
     6541 caaatacgcc cgcagcaccc tccccccgtc catgggaaac gccggaagga ggttgaaggc
     6601 aaacaggaag atgttgagga gccccaggta cgcgaagatg aagaccagca accccgcgag
     6661 tggagggtct gcaatgatac ctgcgaaaac atacagcagt gcagtgcata acaggcccac
     6721 tgcaaggctg gtgagcggcc cacgagtgcc atgggaagct ccacttttgg atcgggagta
     6781 ctctcctcca tggaggagac tcccccgaat atgaggaggg tgatgctgtt gatggggacg
     6841 cccatcctct ttgcaaccag gcaatgtgcg atctcgtgga ccagcacacc gccaaagagc
     6901 ccgagcgcta cgaaggcccc caggatgtaa gggttccagc ccggcgtaag gtagcccata
     6961 tcgatcggga cacggaagag tcccgagaga agctcgaccg taagaactat ctggctcccg
     7021 atgatccagg caaacagggg gatgatcacg aggaatgtcc agtggataag gatgggaatg
     7081 ccgaaaatgg acccgatttt gaacgacccg ttcattaaag gagtatcttt ttaaccatta
     7141 tagtatatat cttccatgat taggatgaga acgcagatta cggagctgtt cgggctgaat
     7201 atctacaccg agaagagcgt gttcgtgggc gaggtcgagg acgtgatcct cgatatcgag
     7261 ggaaagaaga tggagtccct cgtggtcggc aagctcaacc aggatctcgt ggacatcaag
     7321 aactacaagg ggctgaagct tcccttccgt atcatccgga gcatcgggga cattgtcatt
     7381 atccggcacc tccccggcgc cttcaagtcc ggtgacttcg gagaagccgg gaaataaccg
     7441 cacagagccc gggagaagat gtccatgtct cccggcccag gtcttgtttt tccatgaaac
     7501 accgggaact cttcagcaac ctggttgccc tcccgcctat ttttcttcgc cttgacgggc
     7561 ggacctttca ccgactcgcg gagacttggt gcctcgagcg gccgtttgat tcccatttcg
     7621 cagatggcat ggcacgcgtg agcgcccggc tcatatcgga gagcggcctc tctcctgacc
     7681 tcgcattcac cttttcagac gagataagcc tctacttctc ccgcctcccc ttcaacggcc
     7741 gggtggagaa acttgactcc gtatgcgctt cgtttgccgc aagcgccctc gccatcgaga
     7801 tggaaagcac cgttccagtc tcgtttgatg cccgcatcat ccacgtcatc cccgagatgg
     7861 cccccgatta cctgatccag cggcagggag aagcctggag gaaccacctg aatgcctact
     7921 gccagtatgc cctgatgatg gacggcatga gtaggaaaga ggccgcctcg tttttaaaag
     7981 agaagccctc tcaggagatg cacgaactgg cattcgcacg gggaatcaac ctggcccaca
     8041 cgcccacgtg gcagcggcgt ggcatccttg tgcagaaaaa gacctacatg gtaaaagggt
     8101 tcaacccgaa acttggaaag atggcagaga gtatccggag cgaagtggtg acctcccgcg
     8161 acctgccggt tttcgcctcc cccgaggggc gggctctcct ctcctcactg ctccggtgcc
     8221 cttgagaggg aaagcgtcat cgcaataccc tccacgtccc attccttcga catctcgtgg
     8281 cttgtcgctg ctgggcagtc gccgccctga accccgaaag aagtcgccct tatctcctcc
     8341 atgatgccag tcttccatga ctcggcaacc atcccgcaga tgcgggaatc tgtgatgcat
     8401 gcgtctgcag taacaaagtc ctctactttg aggtcaagtt gcctgcgcat ctcctggaac
     8461 cgccggatta tctccctggc atatccctcg gcctcgaggt cagggtcaag cgtcacgtca
     8521 acgtacacgg tccctcccag catgggagca gagaagatac catcgggaag ttgctgtgcg
     8581 aacgtgacct ggcccgctac aatctcgaag cgctctcccc cgttctcaag gatgaacgca
     8641 ttgccggagt cgatggcctg tttcatggcg ttcccgtccg cgttcaggat gagatcccgt
     8701 acccgtggtg cctgctttcc gaatgccgga ccgagtgcct tcatgaccgg ctcagccctc
     8761 cacccgatgc gctcccagtg tccagagaca gcgcggacga ccctcgcgtt cgccctgtca
     8821 cggcagatat ggttgagcga ggagacggcg tcggcgacct cctgcgaatc agcgacgatc
     8881 acgcactccc tgaccggcca gcgtaactta cgctttccag cctggcgggc atttgcctgg
     8941 gcctcgtcaa acgacctgac aatgtccatg gcgcgctcga ggggctcgtc acggagcgtg
     9001 tcgttgcccg cgcaccaatc caacatgtgc acgctttccg ggtctccctc gagacggagg
     9061 ttccggtaca tcgcctccgt gatgtgtggg acaaagggtg aagcgatggc aaggagagtg
     9121 cggagcgtat agtacatcgt ctcgtatgca cgggtctttt cgggagcttc cccttcgagc
     9181 cacatcctgg gcctgaccag ctgcacgtac catcgggaga ggtcttccag gatgaacgca
     9241 atgatagtcc gggaggcacg gtggagctgg cattcggaga gatccgcggt cacctgccgt
     9301 gcgagggagt ttactcggga gacgatccag cggtcctctg gccaaagcga ggggaactcc
     9361 ttcctgacga acccatcgtc ccatgttctg ccgccagaac gcggggaaaa gttgtcaatg
     9421 atcatgtagg ggagcgggaa ccggtacacg ttccagagga tgttcatggc acgggaaacg
     9481 ttcttcacac cgtcccagtt gaacctgagg tcgtcccacg gggcgttctg ggagaggacg
     9541 tacaggcgca ggacgtccac gccctggctc tggacgacct cctcgggggt gacgacattt
     9601 ccgaggctct tgctcatctt ccgcccctcc gcatccaggg caaaaccatg catcaggaca
     9661 ctcctgtaag gcgccctgcc gaacgcaatc gtacttgccc cgagctggct gtagaaccac
     9721 ccgcgggtct ggtcctgccc ctcggtgatg aagtctgcag gccagagtct cttgagcgcc
     9781 tccttctctc ttgggaaccc gagagttgcc catgatgcga ccgcggagtc gaaccagaca
     9841 tcaaagatgt cctcgactct ccgcatggtc ccgccgcacg tgcagggaat ttctaccccg
     9901 tccacccatg gccggtgggg gtccgggacg ggcgaaccgc tcgcatcctc gagttcagcg
     9961 atggtcccga tcacccggtg cgtaccgcac gactcgcagg tccagatcgg gatcgggatc
    10021 ccccagaaac gctggcgcga gatgcaccag tccctcgcct ccttgatcca gtcatagaag
    10081 cgtgcgctcc ctgcccaatc aggataccag gtgactttcc cgacctcctc gagcatgcga
    10141 tccttcaata gcgaggcctt caggaaccac tgcgaggtag ccctgaagat gatcggtgtc
    10201 ttgcagcgcc agcagtggcc ataccggtgg accacgtggt ctgatgcaag gaggtgctca
    10261 ccgagctccc gaaggacgac ctggtccgcg tccttgatga acattgcgga gagcaccccc
    10321 gcgtccccgg taaaatgacc ctgtgcatcg accgggcaga taatgggaag gtcctccttg
    10381 catccaagaa gatagtcgtc ccatccgtgt cctggtgcga tgtggaccat cccggtgttc
    10441 tccatggcaa caaagtccgc ggtaaccacc cggtgggaga tttccgcctg gagcggcacc
    10501 tgatcccgga ggggagagtg gtaagtcctg gcacacatct cccttccgga acatgtttcg
    10561 atcaccttgt agtcctggta cctgcctttc cggaggaccg gttcaaccag gtcttctgcg
    10621 atccagagca cctccttctt cccttccttg accgcctcca cccgtgcata ggtgaattcc
    10681 gggtggaccg cgactgcgac atttgcaggg agtgtccagg gggtggtagt ccatatgacg
    10741 aggtattccc cctcactgcc gtgcacgggg aacttcacga atatggaggg gtctcgttcg
    10801 tcccagtact ccacctcggc atccgcgatc gcagtggcac agcgtggaca ccagttcacc
    10861 acccggaacc cccgctcgag catcccctct tcatccgccc gtttcagggt ccaccatgcg
    10921 gcctcgatat agcccgggtg catggtctgg tatgggtcat cgaagtcaag ccagacaccg
    10981 aggtgcatga actgttcgct catgatatcc ttgtttgtgt cagcaaaagt gcggcagcgg
    11041 tcgatgaatg ccgcgatccc gaaacgttcg atgtccttct tagaggagaa gccgagtgtc
    11101 tgctcgaccc ttacctcgat tggcaggccg tgcatgtcgt agcctgccct tgcaatcacg
    11161 ttcctcccgc acatgcggtg atagcggagc atgcagtcct tgatgatctt gttccaggcg
    11221 gtgccgaggt ggatatggcc ggtggtgtac ggggggccgt ccacgaagaa gaaatccttc
    11281 ccgtccgcat gcaactcctt gaccttcccg taagtgttct cctccctcca gtacttctgg
    11341 accgactcct cgatctcctg ggcctgataa ctgctgctca cttccttcac acgcatcccc
    11401 ctttcctgtc gcttcgccgg gtggagagat agacagtcct gtaatatggg ctgttc
//
LOCUS       DAOQVJ010000002         8778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_8886_length_8778_cov_7.060300, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000002 DAOQVJ010000000
VERSION     DAOQVJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8886_length_8778_cov_7.060300"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..1195)
                     /locus_tag="PKL26_00055"
     CDS             complement(413..1195)
                     /locus_tag="PKL26_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82118.1"
                     /translation="MSARFGTVPRTALILIGIAVLVLAVLLLTDRGDLTSATLVLVAF
                     ACFITGLFLFSFRREQRIGQQPAALMAIPYTNTLSRILADLGVTGTAHFIPVTNDGTF
                     PAPVMQYNPVSGSDLPDRLIEDLTFQTDSGHQGVLTVPSGIPLLSTMERERALSLPST
                     GPELLEAIREVNQDLLELTPKATVTQSGNEIVLSLQDFRLIDGCKAAQDESPENCITA
                     PCPVCSLAGIMLAKGLQKPCSIEQVLVDREAGTVEVRLKVKE"
     gene            complement(1192..1512)
                     /locus_tag="PKL26_00060"
     CDS             complement(1192..1512)
                     /locus_tag="PKL26_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82119.1"
                     /translation="MEPGDLRFLLLCAGEIVVLLAGLAYLPAAIALQVLVPLYLFRDR
                     LDDRPVAFVAAVALGTILAGALLVLFRHTLLPLVITGAFAALALAALILAEARLQRHY
                     GGAA"
     gene            complement(1802..3061)
                     /locus_tag="PKL26_00065"
     CDS             complement(1802..3061)
                     /locus_tag="PKL26_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNS82120.1"
                     /translation="MRFSRCSRGIAALAVVLALLALFFDALPAALVSGGLVVFLAVRG
                     ALFLSGLSSAARSLAVDRALSPLLVRQGSAVTVGTKGRIMVPPGYAAMIGDLPPHGAV
                     VSGATEVAAAGDFRLDYAITPMTTGEHAFRGVILSFSDPFFATTLTIRTGGTLAPSLV
                     VLPSTEYPVGGREGYGERESAAFSAQKSQEIRSFRGYLPGDDPRRIDWKLSAKYDTLY
                     VREYLGRAEFATLLAIDLPDAGLPYDREAFERLKEAAASVIIARMQDRVPFSVLLVSG
                     PNLVSYTPLDRDPQRLVALITKLAPVSRLHYLYRYHSTGSLRRRYALLASSSEPFVQR
                     LSAIAAAPLSHRLPHTFELQVSRVFQALPGSSAHLFTLADRDASHLRILAEQAALRGI
                     RLEVHVPKEGHDPGRLSRSLAAPVAVV"
     gene            complement(3058..4032)
                     /locus_tag="PKL26_00070"
     CDS             complement(3058..4032)
                     /locus_tag="PKL26_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS82121.1"
                     /translation="METRITDQELSALSRAYDRIRGEISRYIVGNQDLLEIIFIGLLA
                     EGHVLVEGIPGTAKSTLVKATAQLMGCEFRRVQCGIDTQPADVIGVRVWDSRQQEFVM
                     KKGPLFSNILLVDEINRLPPKSQSAFIEAMGERQATIDGITMPIERPYIAIATQNPLE
                     REGTFPLIEAQKDRFMFSHRSAFLEGAAELEVIRREHTGELDWPSYFASLAPVLQKAD
                     LLRFSQALRRVHVEEPVLAYIRDLVMATREHSDVELGVSARGSIALVRGAKVRAAIAN
                     RNYVIPDDVKELAPSAFQHRLLLTREAELSGIAPARVVQEVIGATGVP"
     gene            complement(4038..4916)
                     /locus_tag="PKL26_00075"
     CDS             complement(4038..4916)
                     /locus_tag="PKL26_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNS82122.1"
                     /translation="MKPAGALAILLVLLAGIAITAHLSTTDLEFSRYNAGWTGTSGFF
                     SDLERRGARDLGSYDDLVGRSDTTLLLIAPNGSFSPGEIARVREFLREGNNTVFVADE
                     TGESESLLAGLSSRVRVRPGAVCSAEMEFWDYYTVIAYPRGSDPLIANVSSLTFNRPS
                     SVTGGEVLVATTLISWVDVNDNYHLDENETLSSYGLLARERVGNGTLYVLSDPSLCIN
                     GMRNARLSGDNGVFLAQLLSLHPDLLVEQTHSLTGRADPVLAAVLWVKKTTIITISAL
                     IGSVLLVAVAFWRRWI"
     gene            complement(4913..6622)
                     /locus_tag="PKL26_00080"
     CDS             complement(4913..6622)
                     /locus_tag="PKL26_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNS82123.1"
                     /translation="MKRLPLLILVILSAVFLALLTPYVLEPELYTKENGSYTSYHSDP
                     AIMSAYSYERSTDIQAMMQELLDAPEQIVLNIRIRDFEEAQREFEEYKERSEYFSNVV
                     VNLDLSESAVGDFRRENRKNMEALERMINESVRFDEVNRLEIKYRDEENPALLYTVVL
                     EGEAIQNTLKKTSTQFAGREPDLLAVSSELGLNTTKYQEAVDTMEAIVEEDRRAQQQR
                     EERQLPLPPASLSLSVSPGSGRYLDLLRVAGTYTYSSGEQVTLVLDSRDWQTVVPDEK
                     GVFSTTLPIGRIREGQHVLFATTGTLYSNLATFSVIPTDTVLSFWIYQGSDHWDEVLL
                     DGELFAGEIPVAAAPVRILVDDIEGAVFETDADGYYYGSVNLTKGNHTVQAVFDDPGF
                     PLNPSESELREVDLALSVPLLLAVGVGAGALLLASIGALWYVRKRSPPVLPEKAPARS
                     GPLLSRTEPPPSGEVSPGAPSPVDTLIRYKELFAAGDWSGAALVLYRSVIDRFLPADT
                     DTLTPREVAARVAGTPAGAAFGSFVARYEEVRYGGMPLQPQDRLVSHWNEVLAAFEAG
                     GGA"
     gene            complement(6619..6885)
                     /locus_tag="PKL26_00085"
     CDS             complement(6619..6885)
                     /locus_tag="PKL26_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82124.1"
                     /translation="MTEEKKQRAVAVLVIWLLLVIFFMVLGRSLSLEIFFVLWLIGLL
                     VIVELIGPSFVRPSYLRFLTYLVAAGVIVFAVIVAQKVMEILSA"
     gene            7031..7531
                     /locus_tag="PKL26_00090"
     CDS             7031..7531
                     /locus_tag="PKL26_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82125.1"
                     /translation="MNYRHLLLGLLLLAGILATPAAAFTITSLAVNIDENGDADVTVD
                     YSLSWMERFAVYTRIAHPEQQLETALESFSGKEVRIDSVSPARTTLVIEAFADLAEDE
                     SGTTYTTPAMDFTLAEQKVRGYWFSRFVTLDLSPARTVITFPDGYNAPFTNVAYIPSV
                     SHTVGI"
     gene            7696..7893
                     /locus_tag="PKL26_00095"
     CDS             7696..7893
                     /locus_tag="PKL26_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82126.1"
                     /translation="MREKTRENLSLLLFLFVVAVVLWFILSRMRFLVFIPVSLGGFIL
                     FVIIVIVVLYLVLEFLLERSW"
     gene            complement(8198..8431)
                     /locus_tag="PKL26_00100"
     CDS             complement(8198..8431)
                     /locus_tag="PKL26_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HNS82127.1"
                     /translation="MKKQPAIIVPKEKREEMLAAIKRYYSETQGEEIGDLKATLLLDF
                     FTEHLAPGFYNQGVFDACQYMNELIDDALSLQK"
BASE COUNT         1780 a         2767 c         2704 g         1527 t
ORIGIN      
        1 cggcctcagc gcatgttccg gcctttcgac gcaatgatca cagaaccatt cgtaatcgcc
       61 atctttgccg acgacgagat gtattgatcc ctcggaaccg cactttgcgc aggttccata
      121 aatagagagt actaccaaga gatttccccc gaattagtga attgaaaatt atatggctta
      181 acctttttga attgttgttg ataccaacat aaacgtacta ttccctgaaa ggagcacata
      241 gcgagtgtgt cggattgcac agataatatt tatttgctga atgaaaagga atggatctga
      301 tgaaccgggc agatcggatc agttattttc cgtcgatcag gggtagttct gtagcttggg
      361 tgccgtgacc agcaatcgaa gtcgaggtcg atgtcgaagc cgaagcttcc ccttattcct
      421 tcactttgag gcggacctcg accgttcctg cttcccggtc gaccagcacc tgctcgatcg
      481 aacagggctt ctggaggcct tttgcgagca tgatcccggc gaggctgcag accgggcacg
      541 gggcagtgat gcagttttcc ggggattcgt cctgtgccgc cttgcagccg tcgatcaggc
      601 ggaagtcctg gagcgaaagg acaatctcat tgccggactg cgtgacggtt gccttggggg
      661 tgagctcaag gagatcctgg ttcacctcgc ggatcgcctc gaggagctcg ggcccggtcg
      721 agggaaggga gagcgcgcgc tcccgctcca tcgtcgagag gagggggatg ccggagggaa
      781 ccgtgaggac gccctgatgg ccgctgtcgg tctggaaggt caggtcctcg atcaggcggt
      841 cagggaggtc gctgccggag accgggttgt actgcatgac aggggcgggg aaggtgccgt
      901 cgttcgtgac cgggatgaag tgggccgttc ccgtgacgcc gaggtcggca aggatccgcg
      961 agagcgtgtt cgtgtacggg atggccatga gggcggcagg ctgctggccg atccgctgct
     1021 cccggcggaa ggagaagagg aagaggccgg tgatgaagca ggcgaacgcc acgaggacga
     1081 gggtcgcgct cgtcaggtcg ccgcggtcgg tcaggaggag gaccgcgagg accaggaccg
     1141 cgatcccgat gagaatgagg gcggtccgcg ggacggtgcc aaagcgcgcg ctcatgccgc
     1201 ccctccgtag tgccgctgga ggcgggcctc ggcaaggatc agggcggcga gggccagggc
     1261 ggcgaatgcc ccggtgatga ccagcgggag gagcgtgtgc cggaagagga cgaggagggc
     1321 cccggccagg atcgtcccga gggccaccgc cgcgacaaag gcgacgggac gatcgtccag
     1381 ccggtcgcgg aagagataca gcggcaccag cacctggagg gcgatggctg ccggcaggta
     1441 cgcgagccca gccagcagga ccacgatctc cccggcacag aggaggagga accggaggtc
     1501 accaggttcc atgccaggcc acctcccctg caaacgccgg gatccgccca ggacgtcggg
     1561 gattgcatcg cttcacacgg agatgagaag aagaagtcat cggagaccac ctccctctcg
     1621 cagcgatctc atggcagaga tcgctcttcg gaacagcacc cgcggtcatt tcagaccacc
     1681 tccctctcgc agcgatccca tggtagagat tgctcttcgg aacagcaccc gcggtcatcg
     1741 cagatctccc tcctccattg cgccaatcca gccacgctcc tgcagggcac cagcagtcat
     1801 ctcagaccac cgccaccggg gcagcaaggc tccggctgag ccggcccggg tcatgcccct
     1861 ccttcgggac atggacctcc aggcggattc cccggagcgc cgcctgctcg gcgaggatcc
     1921 ggaggtggct cgcatcgcgg tcggccaggg taaagagatg cgcgctggag cccgggagcg
     1981 cctggaagac ccggctcacc tgcagctcga acgtgtgagg gaggcggtgt gaaaggggtg
     2041 ctgcagcgat cgcggagagg cgctgaacga acggttcgga tgacgaggcg agcagtgcgt
     2101 accgccgcct caaggacccg gtgctgtggt accggtacag gtaatgcagc cgggagaccg
     2161 gggcgagctt cgtgatcagc gcaacgagcc gctgcgggtc ccggtccagg ggcgtatacg
     2221 agaccaggtt cggacccgag accaggagga ccgagaacgg gacccggtcc tgcatccggg
     2281 cgatgatcac gctcgctgcc gcctccttca gccgctcgaa cgcttcccgg tcatagggga
     2341 gaccggcgtc cgggaggtcg attgcgagca gggtcgcaaa ctccgcccgt cccaggtact
     2401 cccggacata gagggtgtcg tacttggccg agagcttcca gtcgatccgc cgcggatcgt
     2461 cgccgggcag gtacccccgg aaggaccgga tttcctggct cttctgcgcc gagaacgcgg
     2521 cactctcccg ctcgccgtac ccctcccgcc cgccgaccgg gtactcggtg gaggggagca
     2581 ccacgagcga gggcgcgagc gtcccgcccg tccggatggt gagcgtggtc gcaaagaacg
     2641 ggtcagagaa ggagaggatc accccccgaa atgcgtgctc cccggtcgtc atcggggtga
     2701 tcgcgtagtc cagccggaag tccccggcgg ctgccacctc tgtcgcgccc gataccaccg
     2761 ccccgtgggg cgggagatcc ccgatcatgg ccgcgtaccc gggaggcacc atgatccgcc
     2821 cctttgtccc gaccgtgacc gcgctcccct gccggaccag gagcggggag agtgcccggt
     2881 ccaccgcaag cgatcgggca gcagaggaga gcccggagag aaagagtgct ccccggaccg
     2941 caagaaagac cacgagcccg ccggagacga gcgctgccgg gagcgcgtcg aaaaagagcg
     3001 caaggagcgc aaggacgacg gcaagtgccg cgatcccccg gctgcaccgg gagaacctca
     3061 tggcaccccg gtagcgccga tgacctcctg caccacccgg gcaggagcga tccccgagag
     3121 ctcggcctcg cgggtgagca gcagccggtg ctggaacgcg gacggggcga gctccttcac
     3181 atcgtcaggg atcacgtagt tccggttcgc gatcgcggca cgcaccttcg ccccccggac
     3241 gagcgcgatc gagccgcggg cagagacccc gagctcgaca tcggaatgct cccgggtcgc
     3301 catgacgaga tcccggatgt aggcaaggac cggctcctcc acatgcaccc gccggagcgc
     3361 ctgggagaac cggaggagat cggccttctg cagtaccgga gcgagcgagg caaagtacga
     3421 cggccagtcg agctccccgg tatgctcccg ccggatcacc tcgagctcgg ccgccccctc
     3481 caggaacgcg gaccggtgcg agaacatgaa ccggtccttc tgggcctcga tcagcgggaa
     3541 cgtcccctcc cgttcgaggg ggttctgggt ggcgatcgcg atatacggcc gctcgatcgg
     3601 catcgtgatc ccgtcaatcg tggcctgccg ctcccccatc gcctcgataa aagcgctctg
     3661 gctcttgggg gggagccggt tgatctcgtc cactaaaagg atattagaaa agagcggccc
     3721 cttcttcatc acgaactcct gctgccggga gtcccagacc cggacgccga tcacatcggc
     3781 cggctgggta tcgatcccgc actgcacccg ccggaactcg cagcccatca gctgggcggt
     3841 cgccttgaca agcgtgctct ttgccgtccc cgggatcccc tccacgagca cgtgcccctc
     3901 ggcaagcagc ccgatgaaga tgatctcgag gaggtcctgg ttcccgacga tataccggct
     3961 gatctctccc cggatgcggt catacgcacg ggagagtgcg gaaagttcct ggtctgtgat
     4021 tcgtgtctcc atgatcgtta gatccacctg cgccagaagg ccaccgccac gagcagtacc
     4081 gatcctataa gcgcagaaat tgtaataatc gttgtttttt tcacccagag caccgcggca
     4141 aggaccggat cggccctgcc ggtcagggaa tgcgtctgct ccaccaggag atccggatga
     4201 agcgagagga gctgtgccag aaacaccccg ttgtccccgg aaagccgggc gttccgcatc
     4261 ccgttgatgc agaggctcgg gtcagagagg acgtagaggg tgccgttccc cacccgctcg
     4321 cgggcgagga gcccgtagga ggagagggtc tcgttctcat cgaggtgata gttgtcgttt
     4381 acatccaccc acgagatcag cgtggtggca acgagcacct ccccccccgt gaccgatgac
     4441 ggccggttga aggtgaggga cgagacattg gcaatgagcg gatcggatcc ccggggatac
     4501 gcgatcaccg tgtagtagtc ccagaactcc atctcggcac tgcagaccgc tcccggccgg
     4561 accctgaccc ggctcgagag ccccgcaagc aggctctcag actcccccgt ctcatcggca
     4621 acaaagacgg tattgttccc ctcccggaga aactcccgca ccctcgcgat ctcccccgga
     4681 gagaacgagc cgttcggggc gatcaacaga agcgtcgtat cactcctccc aacaaggtca
     4741 tcgtacgacc cgaggtcccg ggccccgcgc cgctcaaggt cagagaagaa ccccgaggtc
     4801 ccggtccacc ccgcgttgta ccgggagaac tcgagatccg tcgtggagag gtgggcggtg
     4861 atcgcgatcc cggcaaggag gacaaggagg atcgcgagcg cacctgcagg cttcatgcac
     4921 ccccccctgc ctcgaatgcc gcaaggacct cgttccagtg agagactaac ctgtcctgcg
     4981 gctggagcgg catccccccg taccggacct cctcgtaccg ggcgacgaag gatccgaacg
     5041 ccgcccccgc aggggtcccg gcgacccggg cggcgacctc gcggggggtg agcgtgtctg
     5101 tgtccgccgg caggaaccga tcgatcaccg accggtacag gaccagggct gccccgctcc
     5161 agtccccggc agcaaagagc tccttatacc ggatcagggt gtccacgggc gagggagcac
     5221 cgggagacac ctcaccggag ggtggaggct cggtccggga aaggagcggc ccggagcggg
     5281 caggcgcctt ctccgggagc accggggggg atcgcttccg cacgtaccag agcgccccga
     5341 tcgaggcaag gagcagcgcc cctgccccca cccccacggc aaggaggagc gggaccgaga
     5401 gggcaaggtc gacctcccgg agctcgctct ccgagggatt gagggggaag ccgggatcat
     5461 cgaagaccgc ctggaccgtg tggttcccct tcgtgagatt gaccgacccg taatagtacc
     5521 cgtccgcatc ggtctcaaag accgctccct cgatatcatc cacgaggatc cggaccggtg
     5581 ccgcggccac cgggatctcc ccggcaaaca gctccccgtc aaggagcacc tcgtcccagt
     5641 gatcgctccc ctggtagatc cagaacgaga gcaccgtatc ggtcgggatc accgagaacg
     5701 tcgccaggtt cgagtagagc gttcccgtgg tggcaaagag gacatgctga ccctcgcgga
     5761 tccgcccgat cgggagggtg gtggaaaaga cccccttctc gtccggcacc accgtctgcc
     5821 agtcccggga gtcaagcacg agcgtcacct gctcgcctga cgaataggta tacgtcccgg
     5881 ccacccggag gagatccagg taccggccgg acccgggcga taccgagagc gagagggagg
     5941 ccggcggcag cgggagctgc cgctcctccc gctgctgctg cgctctccgg tcctcctcca
     6001 cgatcgcttc catcgtgtcg accgcctcct ggtacttcgt ggtattcagc ccgagctcgc
     6061 tgcttaccgc gaggagatcc ggctcccgcc cggcaaactg ggtactcgtc tttttcaggg
     6121 tattctggat cgcctcaccc tccaggacaa cggtatacag gagggcaggg ttctcctcgt
     6181 cgcggtactt gatctcgagc cggttcacct cgtcgaaccg gacactctcg ttgatcatcc
     6241 gctcaagcgc ctccatgttc ttccggttct cccgccggaa atcccccacc gccgactccg
     6301 agaggtccag gttgaccacc acgttgctga agtactccga ccgctccttg tactcctcga
     6361 actcgcgttg cgcctcctca aaatccctga tccggatatt gaggacgatc tgctcgggag
     6421 catccaggag ctcctgcatc atcgcctgga tatcggtcga ccgctcgtac gaataggctg
     6481 acatgatcgc cgggtccgaa tggtaggacg tgtacgaccc gttctctttc gtgtacagct
     6541 ccggctccag cacgtacggg gtgagcagcg cgaggaacac cgcagaaagg atcaccagga
     6601 tcagcagcgg gagtcgtttc atgcactcag gatctccatc actttctgcg cgacgatcac
     6661 ggcaaacacg atcacgcccg cggcgacaag gtaggtgaga aaacggagat atgacggcct
     6721 gacaaaggaa ggaccgatca gctccacaat cacgagcagc ccgatcagcc agaggacaaa
     6781 gaagatctca agcgagaggc tccggccaag gaccatgaag aatatcacga ggagtagcca
     6841 gatgaccagc accgcaacgg ctcgctgctt cttctcctcg gtcatggaat cttcaccaat
     6901 atagctcgcg gacatcaata aggatgatcc aggtggattc atggagaagg cgatccttta
     6961 ggggagatca ggggaaggcg atcctttatg ggagatgccg taaagagagg agtacaacgg
     7021 tgaatacgct atgaactaca gacacctcct cctgggactc ctcctgcttg ctgggatcct
     7081 tgccacccca gccgctgcct tcacgatcac cagccttgcg gtgaacatcg atgagaatgg
     7141 ggatgctgat gtcacggtgg actactcgct ctcctggatg gagcggttcg cggtctatac
     7201 ccggatcgcc caccccgagc agcagctcga aacagcgctc gaatcatttt caggaaagga
     7261 agtccggatc gattccgtaa gccctgcccg gacaacgctc gtgatcgaag cgtttgccga
     7321 ccttgcggaa gacgagtccg ggaccaccta caccaccccg gcgatggact tcaccttggc
     7381 agaacagaaa gtccggggct actggttctc ccggttcgtc accctggatc tctcccctgc
     7441 ccggaccgtg atcaccttcc ctgacgggta caacgctccc ttcacgaacg tcgcgtacat
     7501 cccctcagtc agccacacgg tcggaatata atcctttttt caacgggaga cctttcccgg
     7561 aacaacaccc gcaccctcat cacggctgcc gatacaagag cctgttgcag caccgatccc
     7621 tcttttctcc ttccgttcgg accaggattc acgaatagaa aagatatatg tcgattcacg
     7681 ccagaactga aacgtatgcg ggagaaaacc cgggagaacc tttccctact gctgtttctc
     7741 ttcgtcgtcg cagtcgtact ctggttcatc ctctcccgga tgcggttcct cgtcttcatc
     7801 cccgtctccc tgggcgggtt catcctgttt gtgatcatcg tgattgtggt cctgtacctg
     7861 gtgctggagt tcctgctcga acggtcatgg taaccacctg ctaaaaaggt gcctctcgct
     7921 ggattgcggg ggagaagaat cgggattttt tttcggcgca ccctcctgtc aatgaaccgc
     7981 cgccggggcg cccatcgggt acggcggcgg gcttatcgca tgcgggtggg cggacagccc
     8041 ccgcacaatc tctcccgttc ctcgatcgtg cccacgccag aagagcgctc ctgaaaagcg
     8101 acccaggaac cgcttccctt ctggagaatc ctggaaccct gtcatcgatt ccagggatga
     8161 gagggaaaac ctcgcgatgc cgggaacggg acttcgatta tttctggagg gataacgcgt
     8221 catcgatcag ctcgttcatg tactggcagg catcgaaaac cccctggtta taaaacccgg
     8281 gtgccaggtg ttcggtgaaa aagtccagca gcagggttgc tttcaggtca ccgatctcct
     8341 ccccctgtgt ttcggaataa taccgcttga ttgcagcgag catctcctct ctcttctcct
     8401 ttggcacgat gatggcaggt tgttttttca tacgatagtg atcccttcat tcaccaatga
     8461 gcggggtgga tcataaatca tcggtttaat cctgcgggag atcactccct gaaggaaccg
     8521 tcaggaccgg tgaggcaacg gagacagggt cgtgaggcaa gaccgcatac acgccagaat
     8581 acccggaaga agaacaatgg ccgccgcctt cccgaaggtg gaaacttgag gagcagactg
     8641 cagccacgta actatctcaa ttcttgaata tatatattca cctgtcagat tactcttctt
     8701 cgcaagaatt aaattaagac cctaagacag actggagctg acgcacagaa tatttgcgtc
     8761 gctaacccga aggtggaa
//
LOCUS       DAOQVJ010000003         8580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_9142_length_8580_cov_4.282933, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000003 DAOQVJ010000000
VERSION     DAOQVJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9142_length_8580_cov_4.282933"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKL26_00105"
     CDS             complement(<1..745)
                     /locus_tag="PKL26_00105"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HNS82128.1"
                     /translation="MIGKEIRIERIMDRNTGRSVVVPMDHGFTLGQIDGLLDMTTTIS
                     EVSEGGANAIVLHKGIVKRGHRRRGRDIGLVVHLSGSTSLNPDPNDKVLVCTVEEAVA
                     LGADAVSIHINLGAPNESRMLESAGKVVKDCNRWGIPLLVMIYPRGKGIDPTSPATVG
                     HCVRVAEELGADLIKTNYTGDPVSFKKIVTSCSVPVFIAGGEKAGDLETLKIIRDSVK
                     AGGAGVCVGRNAFQRENTSAFVRALCRVVH"
     gene            complement(745..1542)
                     /locus_tag="PKL26_00110"
     CDS             complement(745..1542)
                     /locus_tag="PKL26_00110"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HNS82129.1"
                     /translation="MRGKEIRLERIMDRNTGKTIIVPMDHGVTNGPIEGLIDLGRAVD
                     LVATGGANAVLGHVGLSLYGHRKSGRDVGLILHLSASTSIGPDPNEKVLVNSVTSALK
                     MGADAVSIHVNIGADSEARMLSDLGRVAIDCMEWGMPLLAMMYPRGRKIKDEHQVEHV
                     CLAARVAAELGADLVKTVYTGDPDSFRQVTKGCPVPVVVAGGSKTSDLATLEMIEGAM
                     EGGAAGISIGRNAFQHPAPDRFVSAAAEIVHRGCPAEEALEILKEKR"
     gene            complement(1815..2306)
                     /locus_tag="PKL26_00115"
     CDS             complement(1815..2306)
                     /locus_tag="PKL26_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HNS82130.1"
                     /translation="MQCELCGAKIVGPPKTVRVEGAELMVCASCARYGTEVQSPARSS
                     PRAGAAPRAGRTVPARRSRDVFDLIEGEIVEDYGERIRDARMEKGLSQKDLAMQLKEK
                     ELLIKKIEKGDLIPEDEVRRKLEKALDIRLIDSPSEEEGKRGGGKVVPTLGDVISIKK
                     VHK"
     gene            2365..3603
                     /locus_tag="PKL26_00120"
     CDS             2365..3603
                     /locus_tag="PKL26_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HNS82131.1"
                     /translation="MVESARQPQEPQNPEELYRYLLDRVTNMETRNLELREQIRQIES
                     DKRYIETQKIRYEREVRKLKSEIEQLRSPPLVIGTVTDVLDNGRVVVRSSAGPRFMVR
                     SSMCIDPEKLKPGARCTLNQQSLAVIDVLPESYDAQVYGMEVVESPDETYADIGGLNP
                     QINEVREAVELPLKRPDLFDRIGIEPPKGVLLHGPPGTGKTLLARAVAHETNAHFLRV
                     VGSELVQKYIGEGARLVRELFDLARKKAPTIIFIDEIDAVGASRTEATTSGDREVQRT
                     LMQLLAGMDGFERRGDVKIIGATNRIDILDRALLRPGRFDRIIEIPLPDIAGRVAILD
                     VHTRRMRLEESVDLSELASLTEGKNGADIKAICMEAGMFAIRRDRDAVSKEDFLDAIE
                     KIGLDFDRHVCKSGYGAMFA"
     gene            3896..4201
                     /locus_tag="PKL26_00125"
     CDS             3896..4201
                     /locus_tag="PKL26_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HNS82132.1"
                     /translation="MSMFKEFMDFLYEYKVIGLAIAFIMGLAANQLVKSFVDNIIMPV
                     IAVLIPGGDWQTSKLVLGPIVISWGQFLADLIYFIIVAFVIFLIAKKVLREDKVTKK"
     gene            complement(4188..4688)
                     /locus_tag="PKL26_00130"
     CDS             complement(4188..4688)
                     /locus_tag="PKL26_00130"
                     /inference="COORDINATES: protein motif:HMM:NF039541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HNS82133.1"
                     /translation="MLVLFVPKEAVALYRELLSSETSRDALRFYRPVQTPAGVQISMA
                     SLGSALSLASDLRWYVRRYMRDVLFEISDGVYCTRPLAQEIYYGRLAVLHTPWKFRKT
                     YTFESGQVIREDPLIVDVGRVQATARSAGGDVAVLEVWCTEEEFLGKKWSEEKSDDVE
                     DDITSS"
     gene            complement(4826..5989)
                     /locus_tag="PKL26_00135"
     CDS             complement(4826..5989)
                     /locus_tag="PKL26_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HNS82134.1"
                     /translation="MEESVIRNAGPDAELKYQLKLQDLESQVLDLKVKNDEVLRENAQ
                     LKRENNQLKRMPLFVAVVVDLLDRGEVYLRQQGNNQEYLTHVNDEIFRQLKPGTKVAV
                     NNALSIVKIVGNIFDSRVRVMELDESPEITFDQVGGLAKEIEEVREAVEYPLTKPEIY
                     VRVGVEPPKGILLYGSPGTGKTLIAKAVAHQAKATFIRMSGSELVHKYIGEGAQLVRE
                     LFSLAREKSPSIVFIDEIDAVGSTRTNDGTSGSAEVQRTLMQLLAEMDGFDTRGDVRI
                     MAATNRVDMLDPALLRPGRFDRVISIPLPNPEGRKEILKIHSSRMVLGKDVNLEAIVE
                     MTENATGADLQAVCREAGMMAVRREARTISHDDFVRAVRKVRAEVSTDMRMYT"
     gene            6034..6456
                     /locus_tag="PKL26_00140"
     CDS             6034..6456
                     /locus_tag="PKL26_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNS82135.1"
                     /translation="MLIRGRGISKGNGRGRVLTSPEPISFLSGVDPETGMIVEKGHPL
                     EGALITGTVLVFPHGKGSTVGSYIVYALSRNGKAPAAMINLEAEPIIAVGAIIAGIPL
                     VDRLEGGLSLVPDGAIARVDGGEGTVEILEEEAPGETA"
     gene            6507..6917
                     /locus_tag="PKL26_00145"
     CDS             6507..6917
                     /locus_tag="PKL26_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82136.1"
                     /translation="MMLLISGVAICGCTQSPAGSAQSPQGTSRTGMIPGPTVTLPPEH
                     AVEIQINEKDPIYATISVIFAGGKGQVMVTDILVRVTRSDGQVTEKHLEAVKGAELKF
                     DGTKEDDRIEAFVSLTNGNTYKVIDQLVPYRTRG"
     gene            6979..8079
                     /locus_tag="PKL26_00150"
     CDS             6979..8079
                     /locus_tag="PKL26_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HNS82137.1"
                     /translation="MDAHWRFIPAARNSYAALYAACEQEGYILEPVRTPAPDVTCYSL
                     NSLTASRYLPEIRDAPCITIVGGPHATARTEEMARIADYVVVGEGEFVLPALLRSLES
                     GGPVPPGVATKEGFSPAPHCVRLDAYPPFSTMKGYIEISRGCPHACAYCQTPRIFGPH
                     MRHRSIDAVTHAARRFRDARFVTPNALAYGSDGRTPRLEKVESLLSSLRHEEQHIYFG
                     TFPSEVRPEWVTREGIDLITTYCSNIRLHFGAQSGSDRVLSSLHRGHTVQDVISAVEL
                     CLDAGLTPVVDFIVGIPGEEEDDQRMTARLVEWISRRGRVHLHRFIPLPGTPMEKTAP
                     QPLLPEVERLLGALALRGRVTGSWGDPTRRFF"
     gene            8104..>8580
                     /locus_tag="PKL26_00155"
     CDS             8104..>8580
                     /locus_tag="PKL26_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS82138.1"
                     /translation="MMDVLLLADLHGEFGKLESFLELSPDAIFIAGDITNMGPADAVE
                     SLFSRIDVPSFAVPGNCDPREIIDVLEHSDCVCLHGSSLNLGKISIVGIGGSNPTPFN
                     TPFEMTDKQIDDLLTALVRKMERATHNVLISHAPPQGFLDEAGGNHVGSASIRKHMG"
BASE COUNT         1687 a         2674 c         2424 g         1795 t
ORIGIN      
        1 catggaccac gcggcagagg gccctcacga acgcactggt gttctccctc tggaaagcgt
       61 ttcgcccgac gcacacgccc gccccgccgg ccttcacaga gtcgcggatg atcttgagcg
      121 tctcgaggtc tcctgccttc tccccgcccg cgatgaagac cgggaccgag caggaggtga
      181 cgatcttttt gaaggagacc gggtctcccg tgtagttggt cttgatcagg tctgcgccga
      241 gctcttctgc taccctgaca cagtggccca cagtcgcggg cgaggtaggg tcgatcccct
      301 ttccccgggg gtagatcatg acgaggagcg ggattcccca gcggttgcag tccttcacca
      361 ctttccccgc ggattcgagc atcctcgact cgttcggagc tcccaggttg atgtggatgg
      421 agactgcgtc tgcgccgagc gcaacagcct cttcgacggt gcagacaagc accttgtcgt
      481 tcgggtcggg gttgagggag gtgctccccg agaggtggac gacaagcccg atgtcccgcc
      541 ccctcctcct gtgcccgcgc ttgacgatcc ccttgtggag gacgattgcg ttcgccccgc
      601 cctcgctcac ctcggagatg gtcgtggtca tgtccagaag cccgtcgatc tgccccagcg
      661 tgaacccgtg gtccatgggg actaccacgg accggcccgt gttccggtcc atgatgcgct
      721 caatccgaat ctcttttcct atcatcatct cttctccttc agtatctcaa gtgcctcttc
      781 cgccgggcat cccctgtgga cgatctctgc cgcggcgctg acgaaacggt ctggggcggg
      841 gtgctggaag gcatttcgcc cgatggagat ccctgcggca cccccttcca tcgccccctc
      901 gatcatttcc agggtggcaa ggtcgcttgt cttcgagccg ccggccacca ccacaggcac
      961 cgggcagccc ttcgtcacct ggcggaagct gtccgggtca ccggtgtaga ctgtcttcac
     1021 caggtccgca ccgagctccg ccgccaccct ggcggcaagg cagacgtgct ccacctggtg
     1081 ctcgtccttg atcttcctcc ccctcgggta catcatcgcg aggagcggca tcccccactc
     1141 catgcagtcg atggcaacgc gcccgaggtc cgagagcatc cgtgcctccg agtcggcccc
     1201 gatgttcacg tggatggaga ccgcgtcggc ccccatcttc agggcgctcg tcaccgagtt
     1261 caccagcacc ttctcgttcg ggtcgggccc gatcgaggtg ctggccgaga ggtggaggat
     1321 aagccccacg tccctcccgc tcttcctgtg gccgtacagg gagaggccca cgtggccgag
     1381 gactgcattc gcacccccgg tcgccaccag gtcgaccgct ctcccgaggt ctatcagccc
     1441 ctcgatcggt ccgttcgtga caccatggtc cattgggacg atgatcgtct tgccggtgtt
     1501 ccggtccatg attcgttcca ggcgaatttc ttttcctctc atgtaacact cctatccatc
     1561 cagcgcattt gtgtgagaaa tggcagatgg aatcagtaaa aataccagaa ataaccaaaa
     1621 tagcggtgat agcgggaatt agccgcggct attggtttta cgttcgaaag aatggtctca
     1681 acgatgctcg gtgcggacgc caggctagcg gggataaaaa ccctcaccgg cgaaaaaaca
     1741 ctcaccttgc attgaagaac agaatggaac gaacaagtat ataaatatga tgagattgcc
     1801 gggatacccc ggcactactt atggaccttt tttatggaga tgacgtcccc gagcgtgggg
     1861 actaccttcc cgccgccgcg tttgccttcc tcctcggagg gagagtcgat gagccggata
     1921 tccagtgcct tctcaagttt cctcctcacc tcgtcttccg ggatgagatc ccccttctcg
     1981 atcttcttga tcaggagttc cttctccttg agctgcattg ccaggtcctt ctgcgagagc
     2041 cccttctcca tccgggcatc gcggattctt tcgccgtagt cctccacgat ctccccttcg
     2101 atcaggtcaa agacgtccct cgacctcctg gcaggcaccg tccgacctgc acggggtgca
     2161 gccccggccc ggggcgaaga tcttgcgggg ctctggacct cggttccata ccgggcgcaa
     2221 cttgcgcaca ccatgagttc ggccccttcc acgcgtacag tctttggagg gcctacaatc
     2281 tttgctccgc acaattcgca ctgcatactc gtcgcaaaca tctttataga catttttccc
     2341 tatatactta tttgaggcag aaaaatggtg gaaagcgccc gacaacccca ggaaccccag
     2401 aaccccgagg agctctaccg ctatctcctg gaccgggtca ccaacatgga gacccgcaac
     2461 ctggaactgc gcgagcaaat ccgccagatc gagtcggaca agcgctatat cgagacccag
     2521 aagatacggt acgagcggga agtcaggaag ctgaagagcg agatcgagca gttgaggagc
     2581 cctccccttg tcatagggac ggtcacggat gtgctggaca acgggagggt ggtcgtgcgg
     2641 agcagtgccg gcccaaggtt catggtcagg agttccatgt gcatcgatcc cgagaaactc
     2701 aaaccgggtg cccggtgcac cctgaaccag cagtccctcg ccgtcatcga cgtccttcca
     2761 gagagttacg acgcccaggt gtacggcatg gaggtggtcg agagccctga tgagacatat
     2821 gccgatatcg ggggtctgaa cccgcagatc aacgaggtga gggaggcggt cgagttaccg
     2881 ctcaagaggc cggatctctt cgaccgcata gggatcgaac ccccgaaggg agtgctcctc
     2941 cacgggcccc cggggacggg aaagaccctc ctcgcgaggg ccgttgccca cgagaccaat
     3001 gcacacttcc tgcgggtggt cggatctgag ctcgtgcaga agtatatcgg ggagggagcc
     3061 cgccttgtcc gggagctctt cgaccttgcg cggaagaagg ccccgactat catcttcatc
     3121 gacgagatcg acgcggtcgg cgcctcgcgg acggaggcca ccacttcagg cgatcgcgag
     3181 gtgcaacgga cgctgatgca gctcctggcc gggatggacg gattcgagcg gcgtggcgac
     3241 gtcaagatca tcggggcgac caaccgtatc gatattctcg accgggcgct gctccggccc
     3301 ggaaggttcg accgcatcat agagatcccc ctccccgaca ttgcagggcg ggtcgccata
     3361 ctcgatgtcc ataccaggag gatgcgcctt gaagagagtg tcgacctctc tgaacttgcg
     3421 tccctgaccg aggggaagaa cggggccgat atcaaggcca tctgcatgga agcaggcatg
     3481 tttgcaatcc ggagggacag ggacgcggtc tcgaaggagg actttctgga tgccatagag
     3541 aagatcgggc ttgacttcga ccggcatgtc tgcaagagcg ggtacggcgc aatgttcgcc
     3601 tgaatatttt tcttctttta gttttttccc ggttccctac cgcttgacgt ctggaaggca
     3661 ttcaaggggg tggcagcaca ctctcctttc cctaaacttc cgaacgccgt tttgacgttc
     3721 atacgttaag tcaactacct atgcctaatc gtgatgaaaa ttgatcgggg acctgtttgg
     3781 atttgaacaa agaaaaatgg aaataatgca cttggaattg taaaaatcga aatggggtgc
     3841 gccacccaag gttttatcag ccaccccgtg cttttctcaa ctacaggtgt acaacatgag
     3901 catgtttaaa gaattcatgg atttcctcta cgagtataag gtcatcgggc ttgccatcgc
     3961 atttatcatg ggtttggctg caaaccagct ggtcaagtcc ttcgtggaca atatcatcat
     4021 gccggtcatc gcagtgctca tccctggcgg agactggcag acctcgaaac tggtactggg
     4081 tccgattgtt atcagctggg gacagttcct ggccgacctg atctacttca tcatcgtggc
     4141 gttcgtgata ttcctcatcg caaagaaagt gctccgcgag gacaaggtca cgaagaagtg
     4201 atgtcgtcct ccacgtcatc actcttttcc tcgctccatt ttttccccag gaactcctct
     4261 tcggtgcacc agacttcaag caccgccacg tcccctcctg cagacctggc agtcgcctgt
     4321 accctgccaa catctacgat gaggggatct tcccggatca cctggcccga ctcaaacgta
     4381 taggtcttcc tgaacttcca gggagtatgc aggaccgcaa gcctcccata gtatatctcc
     4441 tgcgccagtg gcctggtgca atacactcca tcggagatct caaagaggac atcacgcatg
     4501 tacctcctca cgtaccaccg gaggtcggac gcgagcgaga gtgcgcttcc aagcgacgcc
     4561 atcgatatct gcacgcctgc aggggtctgg accggccggt aaaaccgcag cgcgtccctg
     4621 ctggtctccg aagataagag ctcccggtag agcgccactg cttccttggg gacgaagagc
     4681 accagcacgg cgatacactc ctgtgcgaac tattggtccc gccaggagtt aagatgcagt
     4741 gctcaggcgc ggtcgccacg agatggcgaa tatctcacat tccggggcag acaaggaaaa
     4801 ttgaatggaa aataggggca cttcttcagg tatacatcct catatcagtg ctgacctctg
     4861 cacggacttt cctgaccgcc ctgacaaaat catcgtgcga gatggtccgt gcctcccttc
     4921 gcactgccat catcccggcc tccctgcaga cggcctggag gtccgcccct gtggcgttct
     4981 ctgtcatctc gacgatcgcc tcgagattta cgtcctttcc aaggaccatt cgcgaggagt
     5041 ggatcttcaa tatctccttc ctcccctctg ggttgggaag gggaatggag atgacgcggt
     5101 cgaacctgcc tggccggagc agcgccgggt caagcatgtc cacccggttg gtggcggcca
     5161 tgatccggac atccccgcgg gtatcgaacc catccatctc cgcgaggagc tgcataaggg
     5221 tccgctgcac ttccgcactt cctgaggtcc cgtcgttggt ccgtgtgctc cccaccgcgt
     5281 cgatctcgtc gatgaagacg atggatgggg acttctccct ggcgagcgag aagagctccc
     5341 gcacgagctg ggccccctcg ccaatgtact tgtgcacgag ctcgctcccc gacatccgga
     5401 tgaacgtagc cttcgcctgg tgtgcgactg cctttgcgat gagggtcttt ccggtcccgg
     5461 gggagccgta gaggaggatc cccttcggcg gctccactcc aaccctcacg tagatctctg
     5521 gtttggtcag cgggtattcc accgcttccc tgacttcctc gatctccttt gcgaggcctc
     5581 ctacctggtc gaaggtgatc tccggcgact catcaagctc catcaccctg acccttgagt
     5641 caaagatgtt tccaacaatc ttcacgatcg agagggcatt gttcaccgcc accttggtgc
     5701 ccggcttgag ctggcggaat atctcgtcgt tgacgtgagt aaggtactcc tggttgttcc
     5761 cctgctggcg gagatagacc tcgccacggt cgagcaggtc cacgaccact gcgacaaaca
     5821 ggggcatccg cttgagctgg ttgttctctc gtttcagctg cgcattctcc cgcaggacct
     5881 cgtcgttttt taccttgagg tccaggacct gcgactcgag gtcctggagc ttcagctggt
     5941 atttgagttc tgcgtcgggt cccgcgttcc gtatgacgct ctcttccatg ctaccattat
     6001 atcttgagtt ccctacattt atgtggtgtg atcatgctca tcaggggtcg cggcatctcg
     6061 aaaggcaatg gtcgcggcag ggtgctgaca agtcccgagc ccatctcctt cctctccgga
     6121 gtggacccgg aaaccgggat gatcgtggag aaaggacacc cgctcgaggg cgcccttatc
     6181 accgggacgg tgctcgtatt cccccacggc aagggctcga cggtcggctc ttacatcgtc
     6241 tatgctctca gcaggaacgg gaaagccccg gcagccatga tcaatctcga ggccgagccc
     6301 atcatcgcgg taggagccat cattgcgggg ataccgctcg tggaccgcct agaagggggt
     6361 ctctcccttg tcccggacgg cgcgatcgca agggttgatg gcggggaggg gaccgtggag
     6421 atcctggaag aagaggcacc cggagagaca gcctaatcaa ttcccacttc ctacactcac
     6481 ccatgagact tctgtcctta ctctgcatga tgctgctcat ctcaggggtg gcaatctgcg
     6541 ggtgtaccca gtccccggca ggtagtgccc agtccccgca gggaacgtcc cggaccggga
     6601 tgattcccgg cccgactgtc acgcttcccc ccgagcatgc cgttgagatc cagatcaacg
     6661 agaaagaccc gatatacgcg accatcagcg tcatttttgc ggggggcaag ggccaggtca
     6721 tggtgaccga tatcctggtc cgggtgaccc ggtctgacgg gcaggtgacc gagaaacacc
     6781 tcgaagcggt aaagggcgcg gagctgaagt tcgacgggac gaaggaggac gaccgtatag
     6841 aggccttcgt ctccctcacg aatggcaaca cctacaaggt aatcgaccag ctcgtcccct
     6901 acaggaccag gggttgaccg gggacagggg gagttttcct gtccccctgc cagatccatc
     6961 ctttctccgc tctttataat ggacgcccac tggagattta tccctgcggc gaggaactca
     7021 tatgctgccc tgtacgcggc atgcgagcag gaaggctaca tcctcgaacc ggtccggacg
     7081 cccgcgcctg acgtgacctg ttacagcctc aactcgctca ccgcctcacg ctacctcccc
     7141 gagatacggg atgccccctg catcaccatc gtgggaggtc cccacgcaac ggcacgcacc
     7201 gaagagatgg cccgcattgc cgactacgtc gtggtgggtg aaggcgagtt cgtcctccct
     7261 gccctccttc gctcactgga gtcgggaggg cccgttcccc cgggtgtggc gacgaaagag
     7321 ggcttttccc ccgcgcccca ctgcgttcgc cttgatgcat accctccctt ctcgacgatg
     7381 aagggctaca tcgagatttc ccggggatgc ccgcatgcct gcgcctactg tcagacaccc
     7441 cgtatcttcg ggccgcacat gcgtcaccgg agcatcgatg ccgtcaccca tgcagcccgg
     7501 cggttcaggg atgcacggtt cgtgacgccc aacgcccttg catatggttc agacggccgg
     7561 acaccccgcc tcgaaaaggt tgaatccctc ctctcttcgc tccggcatga ggaacagcat
     7621 atctacttcg gtacattccc aagtgaagtg cgcccggaat gggtgacccg ggaagggata
     7681 gatctcatca ccacctactg tagcaatatc cgcctccatt tcggggcgca atccggaagc
     7741 gacagggttc tttcttcgct ccaccgggga cacaccgtgc aggacgtcat atcggccgtg
     7801 gagctctgcc ttgatgcagg actgacccca gtggtggact tcatcgtggg aatcccgggc
     7861 gaggaggagg atgaccagcg catgactgca cgcctcgtgg aatggatcag ccgcaggggg
     7921 cgcgttcacc tccaccgctt catcccgctg ccggggacac ccatggaaaa gacggccccg
     7981 cagccactcc tgcctgaagt ggaacggctc cttggagcgc tcgcgctgag gggaagagtc
     8041 accggatcct ggggtgaccc tacacgaaga tttttttaaa ggccccctcg aatgaatatt
     8101 gacatgatgg atgtgctttt gctggcagac cttcacggag agttcggaaa gctggaatcg
     8161 ttcctggaac tctccccgga tgccatcttc attgctggcg acatcaccaa tatgggccct
     8221 gcggatgcag tagagtcgct cttttcacgc attgacgtgc cttcgtttgc agtgcctggt
     8281 aactgcgatc cacgggagat catcgacgtc ctggagcatt cggactgcgt ctgcctccac
     8341 ggctcctctc tcaacctggg gaagatctcc attgtaggga tcggcgggtc caacccgact
     8401 cccttcaata ccccgtttga gatgacggac aagcagatcg acgacctcct caccgcgctc
     8461 gtcaggaaga tggagcgggc aacccacaac gtgctcatct cccatgcccc gccccagggg
     8521 ttcctggacg aggctggagg aaaccacgtg ggaagcgcga gcatcaggaa gcacatgggt
//
LOCUS       DAOQVJ010000004         8461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_9312_length_8461_cov_4.570664, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000004 DAOQVJ010000000
VERSION     DAOQVJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9312_length_8461_cov_4.570664"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PKL26_00160"
     CDS             <1..1228
                     /locus_tag="PKL26_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNS82139.1"
                     /translation="GQSRFEPAMFPNASGVAFVSPDCSREVFLAMVGGAKEEILVSIY
                     EFTSPALAGSLEEAHARGVNVKVLIEGGPVGGIPEEEHAVMAQLLGCGIPVRAMQGTA
                     LVHSPYRFLHSKYMVIDSNHVMITSENFKENGFPERGRRGNRGWGLAVTHPGLAEYFR
                     SVYTYDAGGEWAVYAVPRQGDPEIASSRTYTAEFSPYVFEGATVVPVISPDTSGLLHS
                     LIQGARRSVDIEMAYITNETTLCLNPFLADAVDVSRQGIPVRIILDSYYYNIDGKADN
                     DEMVGLLNAMAASEHLPLEARCAYLGPDSVEKIHNKGVIIDREKVLVSSINWNMNSPT
                     FNREAGVIIHHPGVGEYYSLAFERDWEKAGSEKGEKGPDLIKIAVACVVIAALLLFWY
                     WRRRGGRHRHRPRDHV"
     gene            complement(1149..1556)
                     /locus_tag="PKL26_00165"
     CDS             complement(1149..1556)
                     /locus_tag="PKL26_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82140.1"
                     /translation="MKGRACDIMVCDAMVRRYLPAMRAEMVFRLVHERGLSQSDAAKR
                     LGITRAAVSQYLSRKRGDATFELSPDMNALIDRWVMAVSGRDDGITLCDICRCAMKDP
                     VQQETGVFIRGREGDAGAFPPSSSSTRREEGQR"
     gene            complement(1553..1780)
                     /locus_tag="PKL26_00170"
     CDS             complement(1553..1780)
                     /locus_tag="PKL26_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82141.1"
                     /translation="MPEMNLPPSSLTVLHILDEGGAMTHKDLVKKTQLAPRTVRYALK
                     KLKEQHLIIEKFNFKDARQIIYQNRPVAVTA"
     gene            1853..2230
                     /locus_tag="PKL26_00175"
     CDS             1853..2230
                     /locus_tag="PKL26_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82142.1"
                     /translation="MILVRQEKVAVLILVLVVLAVLVSAFVLESVGKGAFSLPYAPDS
                     AEGSLVHHQGMVEESTITASGGHQVLVVSGVKVFIPSEHVQNGWPRPGQEVSLYGTVQ
                     TYRGEREILVASGEDIASLQSPV"
     gene            2395..2958
                     /locus_tag="PKL26_00180"
     CDS             2395..2958
                     /locus_tag="PKL26_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82143.1"
                     /translation="MICIALEIGVHLFLRTAIGYTHIFYVLLVLAAVWYYQKALYVAG
                     ALVAATLATSLFVGDFTWATLLRAAMFLIVTWIVAQISEERDRAKAEILVQKSAIEEK
                     HYVLVGYLAEVALRMKNPIQILHDNMAALLLQLQGDKPDIDSVKMDLAVQISHAEQIM
                     ANFRDLNQAIVEEREEIPVAYRDFLTR"
     gene            2955..3554
                     /locus_tag="PKL26_00185"
     CDS             2955..3554
                     /locus_tag="PKL26_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82144.1"
                     /translation="MNGEATMKIDFSRLGEDRLFLVLSSAERLKVNNIAIIRTLTGQG
                     YRVVVITSNHPSAVLKRLYEAEGIEMARVSFIDTITKYAMGKVPPGQEDCRFINSPSN
                     LTDLGIAITEVLNTLSGEKACLLFDSISTLLIYIPSTNISKFIHFITSKLRLVDASGI
                     FLAVEKGLDPLLLTQLTTYVDGTIDMDAQPPGNEVQAEK"
     gene            complement(3559..4059)
                     /locus_tag="PKL26_00190"
     CDS             complement(3559..4059)
                     /locus_tag="PKL26_00190"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HNS82145.1"
                     /translation="MIEWIILLLIAVATLIAWRYFALRTTLEQRAAALFEAWRAERME
                     TEVKARADLLHREWTLQEEDRVRRDAIGRSGAVIRGKVTEHLTPYFPEFPYDPRDARF
                     LGTPVDLLVFEGLAAGSLERITFIEVKTGKTGALSNREKQVRDCVQKGLVRYEVLHLR
                     GDEKDS"
     gene            complement(4150..4677)
                     /locus_tag="PKL26_00195"
     CDS             complement(4150..4677)
                     /locus_tag="PKL26_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS82146.1"
                     /translation="MTEKKPPEQRIQAEEEARRRRVRGLIKLLQSKYLDDRWKAAEAL
                     ERVGDATAVQPLIDALDDPFVDVSWIAAKTLGQIGDGRAFEPLLRALGSKEKWLRIGG
                     AIGLGRLGDRRAVPLLLESLKDPQALVRKHAAWALGMLGDESSRKALEEAQVDPDKRV
                     REAATEALARLDASS"
     gene            complement(5228..7075)
                     /locus_tag="PKL26_00200"
     CDS             complement(5228..7075)
                     /locus_tag="PKL26_00200"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNS82147.1"
                     /translation="MKRRYLSLIPFEDALEKMRKTAPSPRKVETVLLDSAVGRVLSEA
                     VYAKFSVPGINVASMDGLAVHSSDTRNATDRSPVVLSDAARINTGQPLPPSYDAVIMI
                     EDVWEEDGKWLIRKSAAPWQFVRPAGEDIRKGDLILPRRHLIRAIDIGGLASYGIAFV
                     NVLSVKVGIISTGGELVPLGMTPSPEQGVESNNFFAEAYLSRMGATCKRYPLVPDDRE
                     KIAAQLLLAVRENDLVLLSAGSSAGSTDYSAEILKDSGKLIFHGVGIKPGKPVMMGIV
                     EKTPVLGMPGYPVAAHTVVREFAARLLEYWGMAPHPTYTVKARLGQNLSSDLGYDEFV
                     PVSTGKVDGRYVVIPLSRGFGVQSALLKSNGYIHIPSSAEGLEAKQEIDVTLYENPSF
                     MERSLLMVGSSDTPLQILADCMVDRSITLRCCPTSNLGGILALRAGICHAAPMHIPIL
                     KDHTIPDQLKAQKGLSIRRLVIAEQPFGLATRNAVRLEDLKNLQIMNSQRGTANRLLL
                     DAVLARDGIPVSALKGYENELKNPDMIASNISSMIADAGICTQRSAANAGLRFLPLGI
                     DAYELVMRADTLEDPRVKEMIEMIQSDEFKERLVQNGGFDTGSTGKFRI"
     gene            7407..>8461
                     /locus_tag="PKL26_00205"
     CDS             7407..>8461
                     /locus_tag="PKL26_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82148.1"
                     /translation="MNREFQGKWVDTPEPCYRLDLRLGIPRSRGGEELEWLIGDQGLC
                     RHPDQHPSLILADILSEPHDDPTPIKSAVKKCSLDSGPIKVKLSSTTEKAGDLAALSY
                     TVRPNRGLCEFIRALSDTFVLGAPEGSLVSCMGKTTPWVPQSTVTAHEPWQALVRVKR
                     RPEGYMRNRMDKSTLLQPSKMRHDFPCGERHSRDREGCRVFSDTTQRKPLVFDILRII
                     LRKGRTPVAEYDLYLRSWLAYPESTRGIYERRALREFRIQKGYQMTRPDWSETPRIYV
                     ISDLHLGHENAVPRYKRPFPMGKISEMDRVLIRNWNWTVRKNDVVLFLGDLSFRSQES
                     SESYLQRLEGRIFYLEG"
BASE COUNT         1857 a         2326 c         2392 g         1886 t
ORIGIN      
        1 cgggcagtcg cggttcgaac cggcgatgtt tcccaatgca agcggtgtcg cgttcgtttc
       61 accggactgc tcgagggagg tcttcctggc catggtgggg ggagcaaaag aggagatcct
      121 ggtgagcatc tatgagttca cgagcccggc ccttgccggc tctctcgaag aggcgcatgc
      181 aaggggagta aacgtcaaag tgctcattga aggggggccg gtaggaggca tacccgaaga
      241 ggagcacgcg gtgatggctc aactactcgg gtgcggcatc cctgtcaggg ccatgcaggg
      301 gaccgcgctg gtgcacagcc cttaccggtt cctccactcc aagtacatgg tgatcgactc
      361 gaaccacgtg atgatcacga gcgagaactt caaggaaaac ggcttccccg aacgtggcag
      421 gagaggcaac cggggatggg gactggcagt cacgcaccca ggccttgcgg aatatttcag
      481 gtcggtgtat acatacgatg cggggggaga atgggctgtt tacgccgtcc cgcgccaggg
      541 tgatccggag atcgcctctt cccgcaccta tacggccgaa ttttctccct atgtgtttga
      601 aggggcaacc gttgttcctg tcatatcgcc ggacacgagc gggttgctgc actcactcat
      661 ccagggtgcc cggcggagcg tggacatcga gatggcgtac atcaccaacg agacgacgct
      721 ctgcctgaat cctttccttg ctgatgcggt cgatgtctcc cgccagggca tcccggtgag
      781 gattattctc gactcctact attacaacat cgacgggaaa gccgacaacg acgagatggt
      841 cgggctgctc aacgccatgg cggcatccga acacctgccg ctcgaagccc gctgtgcata
      901 tctcgggcct gacagcgtcg agaagatcca caacaagggt gtgatcatcg acagggagaa
      961 ggttcttgtc agcagcatca actggaacat gaactccccg accttcaaca gggaagcagg
     1021 agtgatcatc catcacccgg gagtcgggga gtattactca cttgcattcg agagggactg
     1081 ggagaaggcg gggagcgaaa agggggaaaa aggccccgat ctcatcaaga tcgcagttgc
     1141 ctgcgtggtt atcgctgccc ttcttctctt ctggtactgg aggaggaggg ggggaaggca
     1201 ccggcatcgc cctcgcgacc acgtatgaac acaccggtct cctgctgcac ggggtctttc
     1261 atggcgcagc ggcatatgtc gcagagcgtg ataccgtcat ccctcccgga gaccgccatg
     1321 acccagcggt cgataagggc attcatgtca ggcgagagtt cgaaagtggc atcaccgcgt
     1381 ttcctgctca ggtactggga tactgcagcc cgggtgatgc cgagcctttt tgcggcatcg
     1441 ctctggctga gtccccgctc gtggacaagg cggaatacca tctcggcccg cattgcaggg
     1501 agatacctgc ggaccatggc gtcacagacc atgatatcgc aggctctccc tttcatgccg
     1561 tcaccgcaac ggggcggttc tggtagatga tctgccgcgc gtccttgaag ttgaacttct
     1621 cgatgatcag gtgctgctct ttcagtttct tcagtgcata tctcaccgtt cgtggtgcaa
     1681 gttgtgtctt cttgaccagg tccttatggg tcattgcccc gccttcatcg aggatgtgaa
     1741 ggacggtgag agatgatgga ggtaagttca tctctggcat gacacacatg ttaacgccgt
     1801 taacatatca aactgtcggt gcttataaag ggcgaggtga tatctctttt ccgtgatact
     1861 tgtccgtcag gagaaggttg cggtcctcat cctggtactt gtcgtcctgg ccgtgctggt
     1921 ctctgcattt gtcctggaat cggttggcaa gggcgccttc tcccttccat acgcccctga
     1981 cagtgcggag ggatcactgg tccatcacca gggaatggtt gaggagagta cgataaccgc
     2041 gagcggtggc caccaggtac tggttgtctc gggtgtgaag gtcttcatac catccgagca
     2101 cgtgcagaat ggctggcccc ggcccgggca ggaggtctcg ctatacggca ctgtgcagac
     2161 ctacaggggt gaaagggaga tcctcgttgc ctcgggggag gacatcgcct ccctccagtc
     2221 accggtatga acgggcatac cgggttcctt ttctgccaat cttaatatgc aagcgacgag
     2281 aatgaaaaga gtgtgaaccc tgaaggaagg ggagaagggg cgggatcgcg cgtaaagtgc
     2341 gatgatggac ctgcaaatac tactcaccgt ctttggatcc tggttctcct cgggatgatc
     2401 tgcatcgccc ttgagatcgg cgtgcacctc ttccttcgca ccgcgatcgg ttacacccat
     2461 atcttttacg tgctgctcgt cctcgcagcg gtatggtatt accaaaaagc gctctacgtg
     2521 gcaggtgcac tcgttgcagc cactctcgcg acaagcctct tcgtggggga cttcacatgg
     2581 gcgaccctgc tacgcgcggc gatgtttctt atcgtaacct ggatcgttgc acagatatct
     2641 gaggagaggg atcgtgcgaa ggccgagatc ctggtacaga agagtgcaat cgaggagaag
     2701 cactacgttc tggtgggata tctcgcggag gtggcgctac ggatgaagaa tcccatccag
     2761 atcctccatg acaacatggc cgcactcctc ctgcagctcc agggggataa gcccgacatc
     2821 gattcagtga agatggacct cgcggttcag atctcgcacg cagagcagat catggccaac
     2881 ttcagggatt tgaaccaggc gatagttgag gagcgcgaag agattcccgt tgcatatcgc
     2941 gacttcctga ctagatgaac ggcgaggcca cgatgaagat cgacttttcg cgactcgggg
     3001 aagatcggct ctttttagtc ctctcttcgg ccgagagact gaaagtgaat aatattgcta
     3061 tcatccgcac tctgacgggg caggggtacc gggtggtggt gatcacatcc aaccacccgt
     3121 cagcggtatt aaaaaggctg tatgaggcag aggggatcga gatggcgcgg gtatccttca
     3181 tcgacaccat cacgaagtac gcgatgggga aggtcccccc tggacaggag gactgcaggt
     3241 tcatcaacag cccatccaac ctgactgacc ttgggatcgc catcacggag gttttaaaca
     3301 ctctgtcggg tgaaaaagcc tgcctgctct ttgattctat cagtaccctc ctcatctaca
     3361 tcccttcgac aaacatctcg aagttcatcc actttatcac cagcaagctc aggctggttg
     3421 acgcctcggg gatatttctc gcggtggaga aggggctcga cccgcttctc cttacccagc
     3481 tgaccacgta cgtggatggg acaatcgaca tggatgctca gcctcctgga aacgaagttc
     3541 aggcagagaa gtaaaaagtc aggagtcttt ttcatctccc ctgaggtgga gcacctcgta
     3601 tcggaccagt cccttctgca cgcagtccct gacctgcttc tcccggttcg agagcgcacc
     3661 ggtctttccg gtctttacct cgatgaaggt gatcctctcg agtgatcctg ccgcaagacc
     3721 ctcgaagaca aggagatcga caggtgtccc gaggaacctg gcatcgcgtg gatcgtaggg
     3781 gaactccggg aagtaggggg tcaggtgttc tgtgactttt ccccttatga cggccccgct
     3841 cctgccgatg gcgtcgcgcc ggacacggtc ctcttcctgg agcgtccact ccctgtgcag
     3901 caggtctgcc cttgccttca cctcggtctc catcctctct gccctccacg cctcaaagag
     3961 ggcagcggct ctctgttcaa gggttgtgcg cagcgcgaag tatctccatg caatcagggt
     4021 ggccactgcg atgagcagga ggatgatcca ttcgatcata ctgcaaaaat gcagccagcc
     4081 cgggaaaaat gcaccgcgtg cagggtgaaa gaatacccaa gctcgcgcgg gtgcggcccg
     4141 ggttggaact catgaagatg catcaagcct ggcgagggcc tccgtagccg cttccctcac
     4201 tctcttgtca ggatcgacct gtgcttcttc gagcgctttc ctggaggatt catcccccag
     4261 catgccaagg gcccatgccg cgtgcttccg gacaagggcc tgcgggtcct tcaatgattc
     4321 gagcagcagc gggacagcac gccggtcgcc gagccgcccg aggccgatgg cgcccccgat
     4381 ccggagccac ttctctttcg aaccgagcgc ccggagaagc ggctcgaatg cccgcccgtc
     4441 acctatctgc ccgagcgtct ttgccgcgat ccaggagacg tccacaaacg ggtcgtccag
     4501 ggcatcgatg aggggctgga cggccgtcgc atccccgacg cgttcgaggg cctctgctgc
     4561 cttccagcgg tcatcaaggt atttactctg caggagcttt atcaaccccc tgaccctgcg
     4621 tctccgcgcc tcctcctcgg cctgtatgcg ctgctccgga ggcttcttct cagtcatctc
     4681 ttgcgcccac cagcaggcag gagtttgaga tcgggtatga taagagttgt gctttcatgc
     4741 cgaagtggtg caaaaacccc tggccccctc ccttgaggga gaaagacgga aaggagtaca
     4801 aaaaattaaa ttaaaaatgt ttacaaagag caataataaa ataataatgt ttatctattc
     4861 tcaaggaata attgtatatt gcatcaaatc atttggtgca gccaaggtaa gtgcaatttg
     4921 gccgtccgta aggtctcaca ggggtattgc cagcaaagat gcgatgttgg aacatgtcca
     4981 tggtcgaatc cacagcattg ctggttcccc cttacgggca cggggaagaa ttgagcgctt
     5041 ttttcggggg tacggtaaaa aatgttgcag aaccgggctg tttttcgctt acgtcatccc
     5101 gcataagacg tgaacggagg gggaacccgg catatcggag tagggggatc gatgtttctc
     5161 accaatccgg gttctcgtag tgcgatcctg gatcctttat cttcatgcag aaaacaacag
     5221 gaggaaatca gatacggaac ttgcccgtgc ttcctgtgtc aaatccccca ttctgaacaa
     5281 gtctctcttt gaactcatca gactgtatca tctctatcat ctcttttaca cggggatcct
     5341 cgagggtatc agccctcatg acaagctcgt acgcatcgat accgagtggc agaaatcgaa
     5401 gtcctgcgtt ggcagccgaa cgttgtgtgc aaatgccggc atccgcgatc attgaggaga
     5461 tgttgctggc gatcatatca ggattcttca gctcgttctc gtatcctttc agagcagaaa
     5521 cagggatacc atccctggcc aggaccgcgt cgagcagcaa ccggtttgcg gtcccgcgct
     5581 gtgaattcat gatctgaagg ttcttgaggt cttcgaggcg taccgcattt cttgtcgcaa
     5641 gcccgaaagg ttgttctgca atgaccagcc ttcggatgga aaggcctttc tgcgctttga
     5701 gttgatcggg gattgtgtgg tctttgagta tgggaatatg catcggtgcg gcatggcaga
     5761 taccggcgcg gagtgcaagt ataccgccaa ggttgctggt ggggcaacac ctcagcgtga
     5821 tactcctgtc aaccatgcag tcagcaagga tctgcagcgg cgtgtccgat gaacccacca
     5881 tcaaaagaga gcgttccatg aatgacgggt tctcatagag ggtgacatcg atctcctgtt
     5941 ttgcttccag cccttcagct gatgatggga tgtgaatgta tccgttcgat ttcaggagcg
     6001 cactctgcac cccgaaaccg cgggaaagag ggatgaccac gtatctccca tccacctttc
     6061 ctgtcgatac cggaacgaat tcgtcgtagc caaggtcaga ggaaaggttc tggccgagcc
     6121 ttgctttcac cgtgtatgtc ggatgtggcg ccatgcccca gtactcgagg aggcgtgcag
     6181 caaactcccg cacgacggtg tgggcggcga cagggtatcc gggcattccc aggacgggag
     6241 ttttctcgac aatccccatc atcacgggtt tgccgggttt gatgcccact ccatgaaaga
     6301 tcagttttcc cgaatctttc aatatctctg cggagtagtc tgtcgaaccg gcagaagacc
     6361 cggctgagag cagcaccagg tcattctccc tgactgccag gaggagctgc gctgcgatct
     6421 tctcccggtc atcaggaaca agggggtatc gcttgcatgt ggcacccatt cgtgacaggt
     6481 acgcttctgc aaagaaattg ttggactcca ccccctgttc gggagaagga gtcattccca
     6541 ggggcacgag ttctccgccg gtggagatga ttcccacctt cacggagagc acattcacaa
     6601 atgcgattcc atacgaagcc aggccaccga tgtctatcgc ccggatcagg tgcctgcggg
     6661 gcaggatcag gtcgcctttc cttatgtctt cccctgctgg cctgacgaac tgccatggtg
     6721 ccgcagattt ccggatcaac cattttccgt cctcttccca gacgtcttca atcattatca
     6781 cggcgtcata ggatgggggg agcggctgtc ccgtatttat cctcgcggca tcagagagga
     6841 cgaccgggga tcgatcggtg gcattgcggg tgtccgaaga gtgcaccgca aggccgtcca
     6901 ttgatgcgac attgatccct ggcactgaaa attttgcata gacggcctcg gaaaggacac
     6961 gaccgactgc gctgtccagg agcacggtct caaccttgcg gggactgggc gcagtttttc
     7021 tcatcttttc aagggcgtcc tcgaaaggta tcagggagag gtatcgtcgc ttcataatat
     7081 cggaacgttg gattaaggga gacccgaacg tgcaggtttg ttagatataa gtattttatt
     7141 ttccttaaat gttgagtatt ctctctttgc acatgatcaa ccaattgttt tcctgttgca
     7201 ggacacagat gccccgcatc catgacttcc ggcaacctcc cgccatcgcc aggcccgatc
     7261 ttgtgagcaa caacgccgtg agagcgcacg ctggatctcc tcctgggagg gccgatcggg
     7321 ggtgccacgc gaatctttaa atgttaatta aaaaatatcg atttcgataa aaattaaaac
     7381 tattaaatta atacatttct gtgcgcatga acagggaatt ccaggggaaa tgggttgata
     7441 cgccggagcc atgctacagg cttgacctgc gccttggaat tccacgttcg aggggtggag
     7501 aggaactgga gtggttgata ggggaccaag gactctgccg tcaccccgac cagcacccct
     7561 ctctcattct cgccgatatc ctgtcagaac cccatgatga ccccacccct ataaaatcgg
     7621 ccgtaaaaaa gtgttctctc gattcagggc caataaaggt taaactcagc agtaccacgg
     7681 aaaaggccgg ggatcttgcc gccctctcat atactgtcag gcccaaccgc ggtctctgtg
     7741 agtttatcag ggcactctcg gatacatttg ttcttggcgc ccccgaaggg tcactggtct
     7801 cctgcatggg aaagaccaca ccctgggtgc cccagagtac ggtgacagct catgaaccgt
     7861 ggcaggcact ggtccgggta aaaagacgac cagagggcta tatgcgaaac cgaatggaca
     7921 aatccactct cctgcagcca tcaaagatga ggcatgactt tccttgcggc gagagacatt
     7981 ccagggatcg cgaaggttgt cgggtcttct cggacactac gcagagaaaa ccactggtgt
     8041 ttgacattct caggataatt ctccggaagg gcaggactcc tgtcgcagaa tacgatctct
     8101 acctgcgttc gtggctcgca tatcccgaat cgaccagggg catatacgaa cggcgggccc
     8161 tccgggaatt ccggatccag aaggggtacc agatgacacg gccggactgg tctgaaacac
     8221 cccggatcta tgtgatcagc gacctccatc tcggccatga aaatgcggtt ccaaggtata
     8281 aaagaccgtt tcccatgggg aagatctctg agatggaccg ggtgctcatc aggaactgga
     8341 actggaccgt caggaagaac gacgtggtcc tcttcctcgg ggatctctct tttcggagcc
     8401 aggaatcctc agagtcctac ctccaacgcc tggagggtcg aatattctac cttgagggga
     8461 a
//
LOCUS       DAOQVJ010000005         8290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_9557_length_8290_cov_5.036551, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000005 DAOQVJ010000000
VERSION     DAOQVJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_8290_cov_5.036551"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKL26_00210"
     CDS             <1..987
                     /locus_tag="PKL26_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNS82149.1"
                     /translation="RKKMEEAWGLKSGSLPDWYGSTLTEQINQCGDPIKAMYIFALNP
                     VVSYPDSNHVMRSLDKLDFLVVQDIFWTETCEHADVVLPGCSFAEKDGTFTSGERRIN
                     RIRKAVDTPGDSKEDWEIFVLLAKKLGLKGFDFKSPKDIWDDMRAVTPSMSGCSYERL
                     EKPESLHWPCPTLDHPGTPILHREKFAAADGLGTFFGLEYRPPAEVADAEYPFTLMTG
                     RLIFHYHTRTQTDRAKVLHYEVPENYVQINTEDAKRLKIANGEKIKVRSRRGESLPIA
                     RVTDEVAPGVLFMAMHFPQGANALTNTALDPLSKMPELKHCAVAVEKTKEGN"
     gene            991..2226
                     /locus_tag="PKL26_00215"
     CDS             991..2226
                     /locus_tag="PKL26_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNS82150.1"
                     /translation="MVKKGDMFYAWTTDANLAKKAECGGAVTQLLKFALESKAVDAVL
                     AVKKGQDLYDAVPVIITDPKELDQTAGSLHCGTLLVSKLVKKYLDGANKMKIAMTVKG
                     CDAMALYELAKRQQVNLDNILMIGVNCGGSVSPVVARKMIKEKFGVDPDKVHKEEIDK
                     GQFIIEYEGGHKGISIDELEEEGYGRRSNCRRCKMKVPRQADLACGNWGVIGDKAGKA
                     TFVEVCSEKGAGLLKAAVGAGVIKTEAPNPKGLEIRGKVENAMFKLADKWRKKDFEGL
                     GNGRDRLKKIKDATSRCVKCYACIEQCPICYCVECSTKKPYFVPPGELPVSFMFHLIR
                     FSHIADSCVNCGQCEENCPMEIPNALFMHANQVELEKMFGHVPGQDMSLPLLALVEER
                     EERDRLLATGSDQIFDIFK"
     gene            complement(2383..3231)
                     /gene="nadC"
                     /locus_tag="PKL26_00220"
     CDS             complement(2383..3231)
                     /gene="nadC"
                     /locus_tag="PKL26_00220"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNS82151.1"
                     /translation="MTKGPEIPLGLLLSFVREDVPAGDVTSDSLLSGQRCTARIVARE
                     EGVIAGLEEASALFQAHGVMVSPRLGDGEPVDAGAVIATLEGPARAVLLVERTALNIL
                     GRMSGIATMTRRLQVILDRVGTGCRIASTRKTAPGLRILDKKAVILGGGDPHRMTLSD
                     GILIKDNHLALVPLEDAVLRAKEYSRYRKVEIEVQTPDEALRAARSGADILLLDNMSP
                     AVIRECLSRLESAGLREGLLVEVSGGVTEENLAGYAMEGVDLISMGALTHSVKNLDVS
                     LEIIHQ"
     gene            complement(3228..4133)
                     /gene="nadA"
                     /locus_tag="PKL26_00225"
     CDS             complement(3228..4133)
                     /gene="nadA"
                     /locus_tag="PKL26_00225"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNS82152.1"
                     /translation="MSVKGRIISLKNQKNAIILAHNYQRPEIQDLADVVGDSLELAQK
                     ARETDSPLIVFCGVLFMAETAKIINPGKKVIIPEKDAACPLADQLSPSLILEAKERHP
                     GAPVVLYINSTAECKAHADVICTSANAVKVVRSLPEREVIVGPDSNLAAYIQGQIPEK
                     EIIAIPPGGHCYVHECFTYEDLESARKRGGEIICHPECRPEIQRGSDMVASTGGMVRL
                     VGSQRGNAAWNILTEREMAYRLKTLYPGHTFHVKEDAICEDMKKTTLENLLLALEYEQ
                     YEVTLPPDIMDRARGAIERMLVIGR"
     gene            complement(4130..4888)
                     /gene="nadX"
                     /locus_tag="PKL26_00230"
     CDS             complement(4130..4888)
                     /gene="nadX"
                     /locus_tag="PKL26_00230"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HNS82153.1"
                     /translation="MIRIGVLGCGNIGHIIAKYRENFEIVALYDQVYPRALELAALSG
                     GNAYQDFESFIASDFEYVVEAASVRAVRQYAEMVLSGEKHLILLSVGALADPNFREDL
                     VRIAEAAGRRIYIPSGAVAGLDNLKVGQISPIRRLLLRTTKSPESLGIRTSERCLVFQ
                     GKSHECIKEFPKNVNVSVALSLAAGRDADVELYADPSVERNIHEIEAEGEFGEIYIRV
                     SNVPSPDNPATSYLAALSILTLLRNLEQPLVVGT"
     gene            5075..6331
                     /locus_tag="PKL26_00235"
     CDS             5075..6331
                     /locus_tag="PKL26_00235"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HNS82154.1"
                     /translation="MEGSRDGHPPEQGVLRTIAQKKVYIKTFGCTYNEGDSRKIAAVL
                     ERQGCTLVSSPEEAGAVIVNTCTVIGATERKVQRELRSLRNYPLYVTGCMAVVQRDAI
                     LSACDPVIFHPDEIHASYAPPGCMPSGRVGIVQICRGCRGSCSYCITRSARGPLVSCG
                     SDEILREVQSLAGQGAVEIRLCGQDVSAWGTDSGSNLGVLLAEIGEVPGNFRVRVGMM
                     NPATLFPVMDQVAGAFGRERIFSFFHLPVQSGSDAVLERMGRGYTAARALAIVERFRT
                     HCPGITLHTDVICGYPGETEEEFRETLAFLSRMQPDKVNVTRYSPRPKTPAARHKDMP
                     DRFKKERSRILRAHAEKIARSRNEAWIGKEVLVLITEILRPGSSMGRTRYYQGVVVSG
                     NHPPGSTMVVRLTGERTYYFIGEPAR"
     gene            6514..7041
                     /locus_tag="PKL26_00240"
     CDS             6514..7041
                     /locus_tag="PKL26_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82155.1"
                     /translation="MDQIEKGYRALLKKIDELDAKKEELSEEVRTREAELMGRMGEMT
                     APLVSRIGMNMLKQGKQDTKGEMYDTRYHDQKMIILGKTDPVEHRPDNISKKVDDQFC
                     VLSEDGKFYELMFSTDEIIVDSFQSPLSPADALQIYGHEIMVMLYRAMRDYMEGQKEL
                     LDALEKTIAFVLAEK"
     gene            complement(7056..7514)
                     /locus_tag="PKL26_00245"
     CDS             complement(7056..7514)
                     /locus_tag="PKL26_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82156.1"
                     /translation="MEKNHVILVVLGLIACVALYFLVDIYAGMIGVILVIALAMSLFI
                     MADSTMLPDVSVRLKEDARGIIVKNRGNAKALSIRLSLIPHDLHYTIASLDEDAENVF
                     PTEMMIDKVKVIAEYQNEKGDLYKRTFTISALDKPDDDLLKPMFPLFKWK"
     gene            complement(7556..7629)
                     /locus_tag="PKL26_00250"
     tRNA            complement(7556..7629)
                     /locus_tag="PKL26_00250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7593..7595),aa:Ile,seq:gat)
     gene            7761..>8290
                     /locus_tag="PKL26_00255"
     CDS             7761..>8290
                     /locus_tag="PKL26_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNS82157.1"
                     /translation="MTTQYLLGNEAIVKGCLESPVHFVTGYPGTPSSEVIDILRSLPE
                     RDFYVEWSVNEKVAFENALAAAWCGLRALCTMKHVGLNVAADPLMTSAYTGVTGGLVV
                     MSADDPFAHSSQNEQDSRCYALFSRLPCLDPSTIQEAHDMVRDAFALSEEFSLPVIFR
                     PTTRICHSKGDTLVGR"
BASE COUNT         1802 a         2293 c         2486 g         1709 t
ORIGIN      
        1 cggaagaaga tggaggaggc atggggatta aagtcggggt ctcttcctga ctggtacggc
       61 tcgaccctga ctgaacagat caaccagtgc ggcgatccca tcaaggcaat gtatatcttt
      121 gccctgaacc ctgtggtctc ttaccctgac tcgaaccacg tgatgcgatc cttggacaag
      181 ctcgatttcc tggttgtaca ggacatcttc tggaccgaga catgcgaaca tgcggatgta
      241 gtcctgccgg gctgctcgtt tgctgagaag gacggcacgt tcacaagcgg ggagcggcgg
      301 atcaaccgca tccggaaagc ggttgacacg cccggagatt ccaaggagga ctgggagatc
      361 ttcgtcctgc tggccaagaa gctcggcctc aagggcttcg atttcaagtc tccgaaggat
      421 atctgggacg acatgcgggc tgtcaccccg tctatgtccg ggtgcagtta cgagcggctg
      481 gagaagccgg agtcgctcca ttggccctgc ccgacccttg atcacccggg aacccctatc
      541 ctgcaccgcg agaagttcgc agcggcagac ggacttggaa ccttcttcgg ccttgagtac
      601 cggcctcccg cagaggtcgc ggatgccgag tatccgttca ccctgatgac agggcggttg
      661 atcttccact accacaccag gacacagacg gaccgggcca aggtcctcca ttacgaagtc
      721 cctgagaatt acgtgcagat caataccgag gatgcaaaga ggctgaagat tgccaacggc
      781 gagaaaataa aggtacggag ccggcgtggc gaatcactcc cgatcgcccg cgtaacagac
      841 gaggtcgcac ctggcgtgct gttcatggcc atgcatttcc cccagggagc aaacgccctc
      901 accaacaccg cgcttgaccc gctgtcaaag atgcctgagc tgaagcactg tgctgtggct
      961 gtcgagaaga ccaaggaggg gaactaaacc atggtaaaga aaggcgacat gttctatgca
     1021 tggaccactg atgccaacct tgccaagaag gccgagtgcg gcggcgcggt gacccagctc
     1081 ctgaagtttg ccctcgagag caaagcggtt gatgcagtgc ttgcggtgaa gaaaggccag
     1141 gacctgtatg atgcagtacc tgtaatcatc accgatccaa aagagctgga ccagacggca
     1201 ggctccctcc actgtgggac gctgctcgtc tcgaaacttg tgaagaagta ccttgacggg
     1261 gcgaacaaga tgaagatcgc catgactgtc aagggatgcg atgccatggc cctctacgaa
     1321 cttgccaagc gccagcaggt gaaccttgac aatatcctga tgatcggggt caactgcggc
     1381 ggctcggtga gccctgtggt cgcccggaag atgatcaagg aaaaattcgg cgtcgacccg
     1441 gacaaggtgc acaaggaaga gatcgacaag ggccagttca tcatcgaata cgagggaggg
     1501 cacaagggga tctcgatcga tgaactggaa gaagaggggt acggccgccg gagcaactgc
     1561 cggcgctgca agatgaaggt cccacgacag gccgatctcg cctgcgggaa ctggggggtt
     1621 attggcgaca aggccggcaa ggcaaccttc gttgaagtct gcagcgagaa gggtgccggt
     1681 ctcctcaagg cggcagtcgg tgcaggggtc atcaagacag aggcaccgaa cccgaaaggc
     1741 ctggagatac gtggcaaagt cgaaaacgcc atgttcaagc tcgccgacaa gtggaggaag
     1801 aaagacttcg aagggctggg caacggccga gaccgcctca agaaaattaa agacgcgacg
     1861 tcccggtgtg tcaaatgtta cgcatgtatc gagcagtgcc ccatctgcta ctgcgtggag
     1921 tgcagcacca agaagccata cttcgtgccg cccggcgaac tccctgtcag tttcatgttc
     1981 catctcatcc gtttctcgca cattgccgac agttgcgtga actgtggaca gtgcgaggag
     2041 aactgcccga tggagatccc gaacgcgctg ttcatgcacg ccaaccaggt ggagctcgag
     2101 aagatgtttg gtcacgtccc cgggcaggac atgtcattgc cgctccttgc cctcgttgaa
     2161 gagagggaag agagagacag gctccttgcg accggaagcg accagatttt cgacatattc
     2221 aaataacacc gggaatgtgg tgggataaaa cccggatccc tcccttccag ggcacagaac
     2281 tccagatcct tttttttgtg tttccgatca cttatggatg atacatgata gcactgtgat
     2341 ctcgctggtg cggggcggtc gtcaaaaaag tgcggagtgc ggtcactgat gaatgatttc
     2401 caggctcacg tcgagatttt ttacgctgtg ggtgagggcg cccatgctga ttaagtcgac
     2461 cccttccatc gcgtagcccg cgaggttctc ctccgtgacc ccgccagaga cctcgacaag
     2521 gaggccctcg cggaggcctg cgctctccag ccgggagaga cactccctga tcaccgcggg
     2581 agacatattg tcaaggagca ggatatccgc cccggaccgg gcagccctga gtgcctcgtc
     2641 aggggtctgc acctctatct ccaccttcct gtacctcgag tattcctttg ccctcagcac
     2701 cgcgtcctcc agaggcacca gtgccaggtg gttgtccttt attaaaatcc cgtcggagag
     2761 ggtcatccgg tgggggtcgc cgccgccgag aatgacggct ttcttgtcaa ggatgcggag
     2821 ccccggcgcc gtcttgcggg tggatgcgat ccggcaaccg gttccaacgc ggtcaaggat
     2881 cacctggagc cggcgtgtca tcgtcgcgat tccgctcatc ctcccgagga tgttgagcgc
     2941 ggtcctctct acaaggagca ctgctctcgc aggcccttcg agggttgcaa tgactgcccc
     3001 tgcatccacc ggctccccgt cgccgagccg tggagaaacc attactccat gcgcctggaa
     3061 aagggctgac gcctcctcca gtcccgcgat gaccccttcc tcccttgcca cgatcctggc
     3121 agtgcagcgc tggccggaga gaagggagtc cgaggtgacg tcaccggcag gtacatcttc
     3181 ccgaacgaaa gagagcagca ggcccagggg aatctcggga cccttcgtca ccggccgatc
     3241 accagcatcc tctcgatagc tcccctggcc cggtccatga tgtccggagg gagggtgacc
     3301 tcgtactgtt cgtactcgag tgccaggagg aggttctcga gggtggtctt cttcatatct
     3361 tcgcatatag catcctcctt aacatggaaa gtgtgtcccg ggtaaagcgt ctttaaccgg
     3421 tacgccatct ctctctcggt caggatgttc caggctgcat tacccctctg gctgccgacg
     3481 agcctgacca tacccccggt ggaggccacc atgtccgatc cacgctggat ctcgggacgg
     3541 cactcggggt ggcagatgat ctcacccccg cgcttccggg cggattcgag atcctcatag
     3601 gtgaaacact catgcacgta gcagtgcccg ccaggaggaa tcgcgatgat ctctttctcg
     3661 gggatctggc cctggatgta ggctgccaga ttggaatcag gcccgacgat gacctccctc
     3721 tcgggaaggg atcggaccac cttcactgcg tttgcagagg tgcagatcac atctgcatgg
     3781 gccttgcatt ccgcagtgct gttgatgtag aggactaccg gcgcaccggg gtggcgctcc
     3841 tttgcctcga ggatcagcga gggagagagc tggtctgcca gtgggcaggc ggcatccttc
     3901 tccgggatga tcactttctt tccgggattg atgatcttgg cggtctctgc catgaagagg
     3961 accccgcaga agacaatgag cggggagtcg gtctcccttg ctttctgggc aagctcgaga
     4021 ctgtcaccca ccacgtcggc gagatcctgt atctcgggcc gctgatagtt atgcgccagt
     4081 atgatggcat tcttctgatt tttcagtgag attatgcgtc cttttacgct catgtcccga
     4141 ccaccagggg ctgctcgagg ttccggagga gagtgaggat agagagggcg gcaaggtagc
     4201 tggtggcagg gttatccggg ctcggcacat tgcttacccg tatgtagatc tctccgaact
     4261 ctccctctgc ctcgatctcg tggatgttcc tctcgactga tggatcagca tataactcga
     4321 catcggcgtc tctccccgca gcaaggctca atgccaccga gacgttcacg ttcttcggga
     4381 actccttgat gcactcgtgg gacttgccct gaaagacgag gcagcgttcc gaggtccgta
     4441 tgccgagcga ctcggggctc tttgtcgtcc tgagaagaag gcggcggatg ggagagatct
     4501 ggcccacctt gaggttgtcg agccctgcga ctgcgccgct cgggatatag atgcgcctcc
     4561 ccgccgcttc agcgatgcgg acgaggtctt cgcggaagtt ggggtctgca agggctccca
     4621 cggagaggag gatgaggtgt ttctctccgg acagcaccat ctccgcatac tggcgcacgg
     4681 cccggacaga ggcagcctct acgacatact cgaaatcact ggcgatgaat gattcgaagt
     4741 cctggtatgc gttccctccg gagagtgcgg caagttcgag ggctcttggg tacacctggt
     4801 catacaaagc cactatctca aagttttcac ggtatttcgc gatgatgtgg ccgatatttc
     4861 cgcaaccaag cacccctatc ctgatcattg tactagagat tgggaatgcc accggttaag
     4921 catttttcca tccatcggga tattcatctg tggttcttaa ggattcgacg tatttttttt
     4981 ttgggataga tccggggtat cgcaggatgc ggattgagcg cctggacggg atgtgaaccc
     5041 tcatctgctc tggcggagag tgtatgagag aggcatggaa ggctcccgag acggccatcc
     5101 ccctgaacag ggggtattgc gcacaatcgc acaaaagaag gtatatatca agacattcgg
     5161 ttgcacctac aacgaggggg acagccggaa gatcgcggca gtgctggaac ggcagggttg
     5221 cacgctcgtg agcagcccgg aagaggccgg ggccgtcatt gtgaacacct gcacggtcat
     5281 cggcgcgact gaacggaaag tgcagaggga acttcgttca ctgcggaatt accccctcta
     5341 tgtcacgggg tgcatggccg tggtgcagag ggatgccata ctctcggcct gtgatcccgt
     5401 catcttccac cccgatgaga tacatgcgtc gtatgcgcca ccgggatgca tgccatcggg
     5461 ccgggtgggg atcgtgcaga tatgccgggg gtgcaggggt tcgtgctcgt actgcatcac
     5521 ccgcagtgca cgcggtcccc tggtgagttg cggaagcgac gagatcctcc gggaggtcca
     5581 gtctctggcc gggcaggggg cggtggagat ccggctttgc ggccaggacg tgagcgcatg
     5641 gggcaccgac tccggatcga acctcggggt gcttctcgca gagattggcg aggttccggg
     5701 caatttccgg gtccgcgtgg ggatgatgaa cccggcgacc ctgtttcccg tcatggatca
     5761 ggtggccgga gcgttcggga gggagaggat cttctcgttt ttccacctcc cggtccagtc
     5821 cgggtcggat gcggtccttg agaggatggg gaggggatac accgctgcca gggcactcgc
     5881 aatagtagag cggttccgca cacactgccc cgggatcact ctccacaccg acgtcatctg
     5941 cggctatccc ggggagaccg aggaggagtt ccgcgagaca ctggcattcc tctcacggat
     6001 gcagcctgac aaggtgaatg tgacccgtta ctctccccgg ccgaaaactc ccgcagcgag
     6061 gcataaggac atgccagacc gcttcaagaa ggaaaggtcc cggatcctgc gggcccatgc
     6121 agagaaaatt gcgaggtcca ggaacgaggc atggatcgga aaggaggtcc tcgttctcat
     6181 tactgagatc ctccggcccg gttcatcgat gggccggacc cggtactacc agggggtcgt
     6241 ggtgagtggg aatcaccccc caggttccac catggttgtg cgcctgaccg gggaaaggac
     6301 gtattatttc attggagaac cggcccggtg atcatcgggc catctcacgc gattttggat
     6361 catggctgtc aggcccacat attgtatcgc atcatatggt ttattgacga tgaaatggat
     6421 gaaaaggaac cattattgca tctgcaaccc actcctcatc taatccccgg agataccagc
     6481 acagattttg aacaaatgga ggttacagaa gcgatggacc agattgagaa gggatacagg
     6541 gcacttttaa agaagattga cgaactcgac gcaaaaaagg aggaactatc agaggaggtc
     6601 cggacccgcg aagctgaact gatgggccgc atgggggaga tgaccgcacc cctggtcagc
     6661 aggataggga tgaacatgct gaagcagggg aagcaggata cgaagggaga aatgtatgac
     6721 acgcggtatc atgaccagaa gatgatcatc ctcggcaaga ccgatcccgt ggagcaccgc
     6781 cccgacaaca tcagcaagaa ggtggatgac cagttctgcg tcctgtctga ggacggaaag
     6841 ttctacgagc tgatgttcag cacagacgag atcatcgtcg attccttcca aagcccgctc
     6901 tcacccgcgg acgcgctcca gatatacggc cacgagatca tggtgatgct gtaccgcgcg
     6961 atgcgtgatt acatggaagg gcagaaagaa ctcctggatg cactggaaaa gaccatcgcg
     7021 ttcgtgctgg cagaaaagta aggtgggagt gaggatcact tccacttgaa gagggggaac
     7081 atcggtttca ggagatcatc atccggcttg tcgagtgccg agatagtgaa ggtccttttg
     7141 tacaggtctc ctttttcatt ctggtattcg gcaatcactt tcaccttgtc gatcatcatc
     7201 tcggttggaa agacattctc tgcatcctcg tcgagtgagg cgatggtgta atggagatcg
     7261 tgtgggatga gggagagccg gatggacagc gcctttgcgt ttccacggtt cttcacgata
     7321 atcccccttg cgtcttcttt cagacgcact gatacatccg gcagcatagt gctgtccgcc
     7381 atgatgaaga gggacatggc cagggcgatt accagtatga ccccgatcat gcctgcatag
     7441 atgtcgacga ggaaatagag ggctacgcag gcgatcagtc ccaaaaccac cagaataacg
     7501 tggttctttt ccatagtatg aactctgctc cgatcagtaa taagaggatg tggaatgggc
     7561 ccgatgtgat tcgaacacat gacctcccgg ttatcagccg ggcgcaccac cgggctatgc
     7621 tacgggccct tgcgttgccg taatagataa ccctccggcc cttttatagg ttttgaatcg
     7681 cgggtctcta tacccgggat ggctatatta catgtactgc cacagattat ctttgagaag
     7741 agagtagatg gtggaggacc atgacgacac agtatctcct tgggaatgaa gcgattgtaa
     7801 aagggtgcct ggaatcacct gtccatttcg tgaccgggta ccccgggacg ccctcatcgg
     7861 aggtgatcga catcctccgt tccctgccgg aacgggactt ttacgtggaa tggtcggtga
     7921 acgagaaggt ggcattcgag aatgctctgg ctgcggcgtg gtgcggtctc cgggcgctct
     7981 gcacaatgaa gcatgtgggt ctcaacgtcg cggcggatcc tctcatgacg agtgcctata
     8041 caggtgtcac cgggggcctc gtggtcatga gcgcggacga cccgttcgcg cacagttccc
     8101 agaacgaaca ggacagccgg tgctatgccc tgttttcccg gctcccctgc cttgacccct
     8161 ccaccatcca ggaagcccac gacatggtca gggatgcatt tgccctctcg gaagagttct
     8221 ccctgcccgt cattttcagg cccaccacgc gcatctgcca ttccaagggg gataccttgg
     8281 ttggaagatg
//
LOCUS       DAOQVJ010000006         8225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_9647_length_8225_cov_4.562791, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000006 DAOQVJ010000000
VERSION     DAOQVJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9647_length_8225_cov_4.562791"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKL26_00260"
     CDS             complement(<1..268)
                     /locus_tag="PKL26_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HNS82158.1"
                     /translation="MYQTTLHLSTCGEGDIVDITPRIREEVKTSRVAEGLVNVFVTGS
                     TAAVTTIEYEQGVLSDLQRALSVVAPDDIPYAHDSRWGDGNGRSH"
     gene            complement(479..>1624)
                     /locus_tag="PKL26_00265"
     CDS             complement(479..>1624)
                     /locus_tag="PKL26_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNS82159.1"
                     /translation="EKAGLEAEIARLQHILSSEETIKAEIRRETMEVSEKFGDARRTQ
                     ISLNFGDISNEDLIEDKTVLVSITTANYIKRTDIDVYRKQRRGGHGITGMTTKEDDVV
                     DSIFTASLKDYLLCFTNTGRVYWLKVYQIPETSRTAKGKPIVNLLNLKDEVVTTVIPI
                     REFRENQFLLFATKLGQIIKIPLKDFSFPRSVGTNAIKLRGSDQLVEVILTDGNKEII
                     LTSRFGQSLRFHEETVRTVGRTAQGVRGMKMKGGDTVVAVTLVEKDHLLTISDVGFGK
                     RTEFDEFRGHGRGTMGVRNIQLERDAVVIASCAVMDTDEIVVMTASGIVIRIHVNEIR
                     VIGRGTKGVKIMRLDENDRIIGIAIVPQDDESGQNGTENGTFTPDDE"
     assembly_gap    1625..1724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1725..2704)
                     /locus_tag="PKL26_00270"
     CDS             complement(<1725..2704)
                     /locus_tag="PKL26_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNS82160.1"
                     /translation="MKTSFIDYAMSVIIQRAIPDVRDGLKPVHRRSLFAMWEMGNTSD
                     KPTKKSARVVGDVMGKYHPHGDASIYDTIVKMAQPFSYRHTLVEGQGNFGSVDGDAAA
                     AQRYTEVRLRPYAEALLEDIEKETVPLVPNYDESLTEPVVLPAKIPNLLVNGTDGIAV
                     GMATKMPPHNLREVCTAVVAYLDNPAITVEELMTSLPAPDFPTGGILMGVEGVRNIFT
                     SGQGRLVVRGVATIEEPKSGSRNPRIIVSELPYQVNKAQWITAIADMVKNKRIEGISD
                     IRDESDKEGIRVVFDLKKDAIPSVVLNNLYKNTQLESSFWAINYAIVENQP"
     gene            complement(2769..4823)
                     /locus_tag="PKL26_00275"
     CDS             complement(2769..4823)
                     /locus_tag="PKL26_00275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HNS82161.1"
                     /translation="MAETYDASHITILEGLQPVRERPAMYIGSTDSRGLHHLVYEVVD
                     NSIDEALAGYCDRIWVSINKNGSVTIEDNGRGIPVDRMEKNGKCALEVVLTVLHAGGK
                     FDKDTYQVSGGLHGVGVSVVNALSSWLDARVYRNGNIYEMRFSQGKTISQCNEREESL
                     EELRARLKHWYLEPEIPEGSAGQAGSIPAKELADDSKLDRNQFLQKFGPKLSGTRITF
                     NPDSSIFETVHFDYDVLAHRLRELAFLNAGLIIFITDERNGDHARYQYLGGLSEFVRY
                     LNEGAECLNPVPIFISKKDEENRLEFDVAIQYTTGYDEKIFSYVNSVNTREGGTHLEG
                     FRSAITRAINTMAKRNGLIKESSSLSLRGEDVREGMTAVVSIKMANPQFEGQTKMRLG
                     NSNVKGIADSLVYSALSEYFDENPKVLQIIVEKALSAAKAREAARNARELARRKGALE
                     SGGLPGKLADCSERNPEKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKAG
                     EHQLLKNAEIQTLVSAIGTGIGENFDISRARYHKIVIMTDADVDGSHIRTLLLTFFHK
                     SMPKLIDAGYIYIAQPPLFSVSKGKVEKYAYREEDMKRLLADMGEKGISVKRYKGLGE
                     MNAQQLWDTTMNPENRIFKKVMIEDAMEANEIFKTLMGKDVEARKDFIKRHAREVKNL
                     DV"
     gene            complement(4926..5234)
                     /locus_tag="PKL26_00280"
     CDS             complement(4926..5234)
                     /locus_tag="PKL26_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82162.1"
                     /translation="MASTFGYGFITNLMHICKHFSLKPEEAFYGAADHLDGFVIPDQF
                     KGTEIEEIADRLRKRIVWHQPGTLDKEEAAEVVRLINRLIVAIDKALGIKDPDLGEFH
                     "
     gene            complement(5273..6100)
                     /gene="cbiQ"
                     /locus_tag="PKL26_00285"
     CDS             complement(5273..6100)
                     /gene="cbiQ"
                     /locus_tag="PKL26_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNS82163.1"
                     /translation="MIEDLFFIEKQTYKKSLIHDLDARVKIVVCFAVIVAMVAVPYSP
                     LVFPVAVTFAVFFAALWVLSRLSPHAYVKRLVMILPFGLFIILVQVFVQNPYYTVFTP
                     VADLPVGLHIYRESIEFATILLLKFLVCVSFIILLSSTTRVQDLLEGAGRLGLPAEFT
                     LTLGMMIRYIFVFGYMYRKINESLATRYFVPFDSHLPYRYRLRTIAYTIGTLFIRSYE
                     QGERTYTAMLCRGYGRESHLYIRKKPLFRKDHAFLAAGLLVAVCTPIFFWVSWIGLI"
     gene            complement(6097..6930)
                     /locus_tag="PKL26_00290"
     CDS             complement(6097..6930)
                     /locus_tag="PKL26_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82164.1"
                     /translation="MHLIETRDLSYTYPGNVRGLDHVNFIAPRNARIAVIGANGAGKS
                     TLFKHFNGIFRPTSGSVLVHGEPITRENIKEVRKFVGVVFQNPDDQIISPTVEQDVAF
                     GPTNLGLDEETVNHRVEEALRVVGIEHLRGRVPHHLSGGEKKRVAIAGIIAMEPQVLV
                     LDEPTAGLDPQGVRDLVGFINSLSRTYGMTVIFSTHDVSLVPEVADIIYVMHRGRVVA
                     EGDCREIFGRSDLLSSVRLDVPVLPKLISSLRDQGVEVSMAYTFTEAERNFLAAYKRN
                     G"
     gene            complement(6982..7329)
                     /locus_tag="PKL26_00295"
     CDS             complement(6982..7329)
                     /locus_tag="PKL26_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNS82165.1"
                     /translation="MGRENLIYFGVIIALVIAVAAPFVASSNPDGLESAFFGVFGAKE
                     VQGSDLDEEAAGAAEEQVQEVTGNTFSFASPFPDYSIEGMEKAGEALIIVIGTLLVLA
                     IAFGLGRVLSRSE"
     gene            complement(7329..7967)
                     /gene="cbiM"
                     /locus_tag="PKL26_00300"
     CDS             complement(7329..7967)
                     /gene="cbiM"
                     /locus_tag="PKL26_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HNS82166.1"
                     /translation="MHIPDAFIPLTQAAVYWVIALIFIALSLRWARNEMKEEKVPLVA
                     VLAAGIFAIQAVNVPIPWGTSGHMMGAALAAIVLGSPFAAIFVLTLVLIVQGVLFGDG
                     GITVMGANIINMGVIGGFVGFYSYKGLNSAIRNPYPAAFIAGWLSLFVAALVCAVEMA
                     IAGTFPLEIGLISMGLYHAVIGVIEGGITAVALYLIYTARPDLISDNAGVAA"
     gene            complement(8029..>8225)
                     /locus_tag="PKL26_00305"
     CDS             complement(8029..>8225)
                     /locus_tag="PKL26_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS82167.1"
                     /translation="RQQISPGDMAKIVSALSDPAIVEDIVELGDMDRPGRLARRLLTR
                     PSLYPLLGILIRSGVRLIYE"
BASE COUNT         1659 a         2335 c         2263 g         1868 t
ORIGIN      
        1 catgcgacct gccgttcccg tctccccagc ggctgtcgtg ggcatagggg atgtcgtcag
       61 gggccaccac cgagagggcc ctctgcaggt cagaaagcac tccctgctcg tactcgatcg
      121 tcgtgacggc cgccgttgag ccggtgacaa agacgttgac cagcccttcc gcaactcggc
      181 tggtttttac ctcctcccgt atccggggcg tgatatccac gatgtctccc tctccacacg
      241 tggagaggtg aagggtagtt tggtacatcg aggagaggag ggagcgggaa gggatgaatg
      301 tgatggagcg ggtgcagtgt gagcctgccg gccgccattt gcgggcgaag gggggtggat
      361 aaggcgtatc tcgtccggga attgggagtc tcgcgttttt caactggaaa aatttcccga
      421 tgcgagaatt gtcgaatatg atgaaaatgt gtgggggtgg ggagaaaaat agagggcctt
      481 actcgtcatc aggtgtaaag gtcccgtttt ccgttccgtt ctgcccgctc tcatcatcct
      541 gcggcacgat tgcaattcca ataatgcggt cgttttcgtc gagccgcata atctttacgc
      601 ccttcgtccc gcgccctatg actcgtattt cgttgacgtg aatccggatt actatcccag
      661 aggcagtcat cactacgatc tcgtcggtat ccatcaccgc acatgacgca atgacaacgg
      721 catcgcgttc cagctggatg ttcctgaccc ccatcgttcc cctgccatgg ccccggaact
      781 catcaaattc ggtcctcttg ccgaacccga catccgagat cgtgagaagg tgatccttct
      841 ccacaagcgt gaccgcaacg accgtatcgc cgcccttcat cttcatgccc ctgacaccct
      901 gggctgtcct ccccactgtc cgcacggtct cctcgtggaa ccggaggctc tggccgaacc
      961 ggcttgtcag gatgatctcc ttgttcccgt cggtgaggat gacctcaacc agctgatcgg
     1021 agccgcgcaa cttgatggcg ttcgtcccga cagatctcgg gaaggagaaa tctttgagcg
     1081 gtattttgat gatctgcccg agtttcgtgg caaacagaag gaactggttc tccctgaatt
     1141 cacgaatggg tatcaccgtg gttaccactt cgtccttgag attgaggaga ttgacgatgg
     1201 gttttccctt cgcagtacgg ctggtctccg gtatctggta gactttcagc cagtaaaccc
     1261 gcccggtatt ggtgaaacag aggaggtaat ccttgaggct cgcagtaaat atcgaatcga
     1321 cgacgtcgtc ctccttggtc gtcattcccg tgatcccgtg cccgccgcgc ctctgtttcc
     1381 ggtacacgtc gatatcggtc cgcttgatgt aatttgcggt agtaatggag acgaggaccg
     1441 tcttgtcttc aatcaggtct tcgtttgaga tgtccccgaa gttcagcgat atctgggtcc
     1501 tccgggcgtc cccgaatttc tcggagacct ccatcgtctc gcgccggatc tccgccttga
     1561 tggtctcctc gcttgacagg atgtgctgga gccgggcgat ctctgcctca agccctgcct
     1621 tctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggctgg ttctcgacaa
     1741 ttgcgtagtt gatcgcccag aaactcgact cgagctgggt gttcttgtag aggttgttga
     1801 gaacgacaga gggaatcgcg tctttcttga gatcgaacac gacacggatg ccctccttat
     1861 cggactcgtc gcggatgtcg gagatcccct cgatcctctt gttcttcacc atgtccgcaa
     1921 tggcagtgat ccactgcgcc ttgttgacct ggtagggaag ctcggagacg atgatacgtg
     1981 ggttccggga accgctcttt ggttcctcga ttgttgctac gccgcgaacc acgaggcgtc
     2041 cctgcccgct cgtgaagatg tttcttactc cctcgacccc catcaggatc ccacctgtcg
     2101 ggaaatcggg agcgggcagc gatgtcatca gctcctcgac ggtgattgca ggattgtcga
     2161 ggtatgccac gactgcagtg cagacttcac gcaggttgtg cgggggcatc tttgttgcca
     2221 ttccgaccgc gatgccgtcg gtgccattca ccaggagatt cgggattttt gcgggaagga
     2281 caacaggttc ggtgagtgat tcatcgtagt ttgggacgag cgggacagtc tccttctcaa
     2341 tatcctcgag gagcgcctct gcatatggcc tgagccggac ttcggtgtac ctttgcgccg
     2401 ccgccgcatc accatcaacg gacccgaaat ttccctgccc ttctacgagc gtgtggcgat
     2461 acgagaaggg ttgcgccatc ttgacgatag tgtcataaat ggaggcatcg ccgtgaggat
     2521 ggtacttccc catgacatct cccaccaccc tcgcactctt cttggtaggc ttgtcactgg
     2581 tgttgcccat ctcccacatg gcgaagagcg agcggcggtg aacgggcttc aaaccatcac
     2641 ggacatcagg gatggcccgc tggatgatga cgctcatcgc ataatcgatg aaggaggtct
     2701 tcatctcctt ctcgatgctt acctcctcca ccctctcggt cactgcatcg tcccggggaa
     2761 ggtccggatc agacgtcaag gttcttcacc tcccttgcgt gccgcttgat gaagtccttc
     2821 ctggcttcca cgtccttgcc catcaatgtc ttgaatatct cgtttgcctc catcgcatcc
     2881 tcgatcatga ccttcttgaa gatccggttc tcgggattca tcgtcgtatc ccagagctgc
     2941 tgggcattca tctctccaag acccttgtat ctcttgaccg agatgccttt ttcacccata
     3001 tcggcaagga gtctcttcat gtcctcttcc ctgtatgcgt atttctcgac cttgcctttc
     3061 gagacggaga agaggggagg ctgtgcgatg tagatatacc cggcgtcgat cagcttgggc
     3121 atcgatttat ggaagaacgt gaggaggagg gtccgtatgt gtgagccgtc cacatcggca
     3181 tccgtcatga tcacgatttt atgatagcgg gcgcgggata tgtcaaagtt ctccccgatc
     3241 ccagtcccga tcgcggacac cagggtctgt atctccgcat ttttcaggag ctggtgctcg
     3301 ccggcctttt cgacattcag gatcttcccg cggagcggca ggattgcctg gaacttcctg
     3361 tcccttccct gcttcgcact gcctccggca gaatctcctt ccacgatgta gatctcgctc
     3421 ttttcgggat tccgctcgga gcagtcggcg agttttccag ggaggccgcc actttccagg
     3481 gctcccttcc ggcgtgcgag ttcccgggca ttccgggcgg cctcgcgggc tttcgccgcg
     3541 gagagcgctt tttcgacaat aatctggagg actttcggat tctcgtcgaa gtattcggaa
     3601 agtgccgagt agacgaggga atcggcgatc cccttgacgt tgctgttccc gaggcgcatc
     3661 tttgtctgtc cctcgaactg ggggttcgcc atcttgatcg agaccaccgc ggtcattccc
     3721 tccctgacat cctccccccg cagcgagagg gaggaacttt ccttgatcag gccgttcctt
     3781 ttcgccatcg tattgatagc gcgggtaatt gcactcctga acccttcaag atgcgtcccc
     3841 ccttcgcggg tattgacgga attcacatac gagaagatct tctcatcgta cccggtggtg
     3901 tactggatgg caacatcgaa ctcgagcctg ttctcctcat ccttttttga gataaagata
     3961 ggtacgggat tgaggcattc ggccccttcg ttcagatatc tcacgaactc cgacaggcct
     4021 cccaggtact ggtaccttgc atgatccccg ttcctctcgt cagtgatgaa gatgatcagc
     4081 cctgcgttca ggaacgccag ttctcttaat ctgtgggcga ggacgtcgta atcaaaatga
     4141 actgtctcga agattgacga atcggggttg aacgtgatcc tcgttcctga tagtttcgga
     4201 ccgaatttct gcaggaactg gtttctgtca agtttactgt cgtctgccag ttcctttgcg
     4261 ggaatcgatc ccgcctgacc tgccgatcct tcgggtatct ccggttccag gtaccaatgc
     4321 ttcaaacggg cacgcagttc ttcgagcgac tcctcgcgtt cgttacattg tgaaatggtc
     4381 tttccctgcg aaaaccgcat ctcgtaaata ttcccgttcc tgtatacacg ggcatcgagc
     4441 cagcttgaga gtgcattcac caccgagacc ccaacaccgt gcaggcctcc ggacacctgg
     4501 taggtgtcct tatcgaattt tcctcccgca tggaggacgg tgagcacgac ttccagcgca
     4561 cattttccat tcttttccat ccggtcaacg gggattcctc tcccgttatc ctcgatggtg
     4621 actgaaccat tcttattgat actgacccag atgcggtcac aataccctgc gagggcttcg
     4681 tcgatggagt tgtccacgac ctcgtagaca aggtggtgga gcccgcggga gtcggtgctc
     4741 ccgatgtaca tggcgggccg ctcccgcacc ggctgcaacc cctccagtat agtaatatga
     4801 gaggcatcat acgtttcggc cattgcttcc ccctgcgaaa aaatacgctg cgtgccgcaa
     4861 aacggacgtc acatacagta ttcgactcct gaccacttaa taatgaaggt gggaatgggc
     4921 cgcagtcagt ggaattcgcc cagatccggg tcttttatcc caagggcctt gtcgattgcg
     4981 acgatcagcc tgttgataag acggaccacc tctgcggctt cttctttatc aagggtgcca
     5041 ggctggtgcc agacgatccg cttgcgaagc ctgtctgcga tctcctcgat ctcggtcccc
     5101 ttgaactggt cggggatgac gaagccgtcc aggtggtccg ctgccccgta aaacgcctct
     5161 tcaggcttta aggagaagtg cttgcagatg tgcatgaggt tcgtgatgaa tccatacccg
     5221 aatgtactgg ccatatgagg aggaatgtcg cgggaaggaa aaaaaggtgc gatcagatca
     5281 ggccgatcca ggagacccag aagaagatgg gggtgcatac tgcgacgagg agacccgccg
     5341 ccaggaatgc atggtccttc cggaacagtg gcttttttct gatatagaga tggctctccc
     5401 tcccgtatcc ccggcagagc atggccgtgt aagtccgctc gccctgttca taggagcgga
     5461 tgaagagggt gccgatggtg taggcgatcg tccggaggcg gtaccgataa gggaggtgcg
     5521 aatcaaacgg cacaaaatac cgggtggcaa gcgattcgtt gatcttcctg tacatgtatc
     5581 cgaagacgaa gatataccgg atcatcatac cgagcgtcag ggtgaactct gcaggcaggc
     5641 cgagtctgcc tgccccctcc aggagatcct ggacccgcgt ggtggacgag agcaggatga
     5701 tgaaggagac acagacgagg aacttgagga ggaggatggt cgcaaactcg atggactcac
     5761 ggtagatgtg cagccccacg gggaggtccg cgacaggtgt gaataccgtg taatagggat
     5821 tctgcacgaa tacctgcacg aggatgatga agaggccgaa cgggaggatc atcacgagcc
     5881 gcttcacgta cgcgtgggga gagaggcgtg agagaaccca gagtgctgca aaaaaaactg
     5941 cgaatgtcac tgccaccggg aagacaagag gcgaatacgg gaccgccacc atcgccacaa
     6001 tcaccgcaaa acatacaact atcttgaccc tggcgtcaag gtcgtgaatg aggctctttt
     6061 tgtaggtctg cttctcgata aagaacaggt cttcgatcat ccattcctct tgtacgcggc
     6121 aaggaagttg cgttcggcct cggtgaatgt ataggccatg cttacctcca ccccctggtc
     6181 acgcaggctg ctgatgagtt ttgggagcac cggcacatca agcctcaccg atgagaggag
     6241 gtcagacctg ccgaagatct cccggcagtc cccttcagcc acaacccttc cgcggtgcat
     6301 cacgtagatg atatctgcca cttccgggac gagagatacg tcatgggtgg agaagatcac
     6361 cgtcataccg tatgtccgcg agagggagtt gatgaacccg acgaggtcac gcaccccctg
     6421 cggatcgagg cccgccgtgg gttcgtcgag gacgagaacc tgcggttcca tcgcgataat
     6481 cccggcaata gccacccgct tcttctcgcc cccgctcagg tggtggggaa cccggcccct
     6541 caggtgctcg atcccgacca ccctgagtgc ctcctcgacc cggtgattga cagtctcttc
     6601 gtcgaggccg aggttggtgg gcccgaaggc cacgtcctgc tctacagtgg gtgagatgat
     6661 ctggtcgtca gggttctgaa agaccactcc cacgaacttt cggacctcct ttatgttttc
     6721 gcgggtgatc ggttcgccgt gaaccaggac gcttccgctc gtgggtctga agatgccgtt
     6781 gaagtgcttg aagagcgtgc tcttgcctgc cccgttcgcg cctatcaccg ctatccgggc
     6841 attccggggc gcgatgaagt tcacgtggtc cagtcctctg acgtttcctg gataggtata
     6901 ggagagatcc ctggtctcga tcaggtgcat ggaatttgta tcccgcgacg ttaagacgtg
     6961 aaaaataata gaggtattct tttattcgga acgggagagc acccgcccga gaccgaatgc
     7021 gattgccagc acgaggaggg tcccgatcac gatgatcagt gcctcacccg ctttctccat
     7081 cccttcaatg ctgtagtcgg ggaacgggga cgcaaacgag aacgtgttcc cggtgacctc
     7141 ctgcacctgt tcctcggcgg ccccggcggc ttcctcgtcc aggtccgacc cctggacctc
     7201 ctttgcccca aagaccccga agaatgcact ctccaggcca tcggggttgc ttgacgcgac
     7261 gaacggggcg gccaccgcga tgacgagcgc aatgatcacc ccgaagtaga tgaggttctc
     7321 ccggcccatc aggctgccac ccccgcgttg tcgctgatca ggtcgggcct tgcggtgtag
     7381 atgaggtaga gggctaccgc ggtgattccg ccctcaatca ccccgatgac cgcatggtaa
     7441 agccccatgg agatcagtcc gatttcgagc ggaaaggtcc cggcgatcgc catctccacc
     7501 gcgcatacga gcgccgccac gaagagcgag agccacccgg cgataaaggc ggcagggtac
     7561 gggttcctga tggcggaatt cagtcccttg taactgtaga aacccacaaa gccccctatc
     7621 actcccatgt tgatgatgtt tgcacccatt accgtgatgc ccccgtcccc aaagaggact
     7681 ccctgtacaa taaggacgag cgtgaggacg aagatggctg caaaggggga gccgagcacg
     7741 attgccgcaa gggccgcacc catcatgtgc ccgctcgttc cccagggaat cggcacattg
     7801 acagcctgga tggcaaatat gccggcagcg aggaccgcca ccaggggaac tttctcttct
     7861 ttcatctcgt tcctggccca cctgagtgag agggcgataa agatcagcgc gatgacccag
     7921 tacaccgctg cctgggtgag tggaataaac gcatcgggaa tgtgcacgat attcaccaga
     7981 gtattactga ctagcccaat cgtattaatt gttagataaa aaatcttact attcgtaaat
     8041 aagacgtact cccgaacgga tgagtatgcc aagaagagga tagagagagg ggcgggtgag
     8101 aagccggcgg gccaacctgc cgggccggtc catatcccca agttcgacaa tatcttccac
     8161 gattgccgga tcagagaggg ctgagacgat cttcgccatg tcccccggcg agatctgctg
     8221 cctca
//
LOCUS       DAOQVJ010000007         7580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_10702_length_7580_cov_4.514419, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000007 DAOQVJ010000000
VERSION     DAOQVJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_7580_cov_4.514419"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKL26_00310"
     CDS             <1..318
                     /locus_tag="PKL26_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNS82168.1"
                     /translation="EIADRLCCPLRAWGSPRLPGVLAVVGGSGFSPDLIAEAAGEGAD
                     SLLSAELRHSVARGSPIPLVEATHYALEAPAMKALAQKMGWAYIEDPPLLHTWIPKSS
                     GAC"
     gene            288..611
                     /locus_tag="PKL26_00315"
     CDS             288..611
                     /locus_tag="PKL26_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNS82169.1"
                     /translation="MDPEEFWRLLKKEGVLTPRLSDEFVRIYGDRGQKALHALKNGMV
                     RKYLDFVVVAGTSQSYVVEEDFCTCRDFVFRGKCCWHLLAARLASICGGYAEREEWYQ
                     DTWDM"
     gene            674..3415
                     /gene="alaS"
                     /locus_tag="PKL26_00320"
     CDS             674..3415
                     /gene="alaS"
                     /locus_tag="PKL26_00320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNS82170.1"
                     /translation="MLEEEYQLEYFKTHGLTRKICKKCGSAFWTRNPDTEICGDAPCE
                     TYNFIGAPIFRRHTVDEMREAYLSFFEKNGHTRINRYPVAARWRDDIYLTIASIADFQ
                     PWVTGGVVPPPANPLTISQPCIRLNDLDSVGRSGRHLTLFEMMAHHAFNSDVEEIYWK
                     ERTLELCEQFISSIGGDLSRVTYKEHPWIGGGNAGPSLEVLIGGLEVATLVFMNLGRQ
                     KTGKPGIDLAGEMYYPMSNWIVDTGYGLERFVWASRGSPTIYDAVFPEMVSRVMEAAG
                     LAHALEDKEYTKILSLNAKFAGVMDISGTNLYQLRKKVATAIEVPIERLDKMITPIEK
                     VYAIVDHTRCLAYMLGDCIVPSNVREGYLVRLVLRRTLRMMNDLGIKEPLADLIEQQM
                     RIVGTSSFEQDTLIVREIVEREVEKSNATMERGTKIVQRIARSYKNKRERVPLKEVIT
                     LYDSHGIPPEIVREVALAEGAVVDLPDNFYSLIADMHSESGPVAEEDPFARYRDRISA
                     LPATRKLFYEQPCDMEFDAMVLDFFDGYVVMDQTLFYPEGGGQPPDCGMLMTAENMVR
                     VDDVKKVGDVIVHHVTGGTLRRGDRVKGIVDEERRWSLMRHHTATHLLLHASKEVLGA
                     HIHQAGAQKGLESSRLDIRHYKHITPDELKKIEIAANRMVMADQVVDVKHLDRTRAEQ
                     LYGFALYQGGVPPGREIRIVQVAGDVEACAGTHCRSTGDIGFVKVIRVEHIQDGIERL
                     EFAAGLAAVEYVHRIESLVAQAADVLSVQNENLPATVSRFFSEWKGQKKEIERLRQNL
                     VELEIKNLTLDTVNSTPLLVRQIDLSGKELAAIGGEISGKGGVALLANRSEPATVLLM
                     SGTEKVNAGEIIGQVCSLLGGKGGGSRTMAQGGGPDVQQVDLALKVGRERILEALRG"
     gene            3437..4078
                     /locus_tag="PKL26_00325"
     CDS             3437..4078
                     /locus_tag="PKL26_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82171.1"
                     /translation="MTDTLHDDAGDRGAGAPAGEVVVLQPGDERAQKIGKAIASQAAN
                     DILHALQERPKTAGDLASTLAMPMGTVKYHIENLLDAGLLEIRETRYSVKGREIKVYG
                     LKDQLLIVAPRVQNIRSLLLKYATLFGIIILASVAAYAMLPILSAPPGMGGEDVAKFQ
                     GERSVGIMAEPAPSAVAPVQGLALDPVLAFFLGGCLVIILLIIYEVAVWRRLK"
     assembly_gap    4081..4180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4232..5032)
                     /locus_tag="PKL26_00330"
     CDS             complement(4232..5032)
                     /locus_tag="PKL26_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82172.1"
                     /translation="MRSAVFFLLGLFVLAGSVNAYVLTISAPSEIRAGAPLEVVGNTT
                     FPVGTQFDLVIYRLQPTTPAEVTRTIIIVDESKSFQASFPTTGMEAGDYKVEARFLTD
                     PGSKLGSDSVTLRVFRITDRSGEIVLTVPTDQVLGEALRVEGYIPKAGVTTITLKVSG
                     PQGAVLPPQDIRTTTRTGRDEGYFMKMFPVSERGNYYVDFYDVKGFMTTVKFMVNAPP
                     PGTEEVVTPGATASPTESAIPTRTPFPVAGCCAALIGAGLIARRVRRI"
     gene            5315..5782
                     /locus_tag="PKL26_00335"
     CDS             5315..5782
                     /locus_tag="PKL26_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82173.1"
                     /translation="MHKSYLSQLGIKEGDDVEISLGEEAKPVVVAVFADTLVEEGHIR
                     LSPEDIAAIGAAPGSSVIVTRRPPLADRVKTGAETTAEKVKGGAAQAAEKIRGGAPEA
                     AGKVKESATQAAEKVKEGASQAAEKVKEGASVAKESIEKSTETVKKKLQDKDL"
     gene            5799..6182
                     /locus_tag="PKL26_00340"
     CDS             5799..6182
                     /locus_tag="PKL26_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82174.1"
                     /translation="MADIWVTVNQSFPLLLIILGLIIVYFIIREVRIMYTKTRIAKMD
                     LEKEKLQLIKADLEQKGHPFFRVSPAKQEELRSLDEENTMLETDIFAQQSAVEKRIQR
                     LESKVKLTKLDHMIEKIKKEEDRLG"
     gene            6189..6497
                     /locus_tag="PKL26_00345"
     CDS             6189..6497
                     /locus_tag="PKL26_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82175.1"
                     /translation="MTVYETPGAIGEKHSFIDSIKDIPGYIDQRLPTMDKDLDAYFDQ
                     NLPAIIEEWGLLGSVHLKELERRLSRVHQQIDFLEKGRTSLEARAAKLEKELKKLEGL
                     "
     gene            6494..6715
                     /locus_tag="PKL26_00350"
     CDS             6494..6715
                     /locus_tag="PKL26_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82176.1"
                     /translation="MMGLDYMERYIDRRMKDLIEDWNLATRNDLGDLYSRLHAIEDEA
                     RMCAGFEATATAKMTELEKRLEHLKGAKR"
     gene            6712..7050
                     /locus_tag="PKL26_00355"
     CDS             6712..7050
                     /locus_tag="PKL26_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82177.1"
                     /translation="MTVTEALLIFLLIILIGFIIYYYFRGSSGKLEITRPMESRLDEY
                     LDRRFDSMIDEWSLLRKPQAQKFRDEKLADLSRDEKRMASLSEFERQFGEQMANLEER
                     LETLEKQVIR"
     gene            7019..7330
                     /locus_tag="PKL26_00360"
     CDS             7019..7330
                     /locus_tag="PKL26_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82178.1"
                     /translation="MKLWKSRSSGNKRRESEKEDLVSRLKKDVPMMRGQGEPPDKDPA
                     HFSGFLCDLCNSPFAYHELRQCILCGRWACSSCWTPEFYVCNSCNGMITLHTMGDKSS
                     R"
BASE COUNT         1741 a         2055 c         2229 g         1455 t
ORIGIN      
        1 gagatcgccg accggctctg ctgcccgctg cgggcatggg ggagcccacg actcccgggc
       61 gtgctcgcgg tggtgggagg atcaggcttc tcccctgatc ttatcgcaga ggcggcaggc
      121 gaaggtgcag attccctcct ctcggcagag ttgcggcact cggtggccag gggctctccc
      181 attcctctcg tggaagcaac gcattatgca ctggaagccc ccgccatgaa agcactcgct
      241 cagaagatgg gctgggcata cattgaagat cccccgcttc tccatacatg gatcccgaag
      301 agttctggcg cctgctgaaa aaggagggcg tcctcacccc ccggctctcc gacgaattcg
      361 tccgtatata cggagaccgc ggacagaaag ccctccatgc cttgaagaac gggatggtca
      421 ggaagtacct cgatttcgtg gtcgtcgcag gcacgtcgca gagttacgtg gtggaagagg
      481 atttctgtac ctgcagggac tttgtattcc gcggaaaatg ctgctggcac ctccttgcgg
      541 cccgccttgc aagtatatgc gggggttatg cagagcgcga agagtggtac caggacacct
      601 gggatatgtg aagcagcgga atccaacaat ttttatcgtg aaaaaccgaa taaattgaga
      661 acacgagggt tttatgctcg aagaggaata ccagctcgaa tattttaaga cccacggact
      721 cacgcggaag atatgcaaga agtgcggatc tgccttctgg acaaggaatc cggataccga
      781 gatctgcggc gatgctccct gtgagaccta caattttatc ggtgccccga ttttcaggcg
      841 ccatactgtg gacgagatgc gggaggcata cctctcattc ttcgaaaaga acggccacac
      901 cagaatcaac cgctaccctg tcgcggcacg gtggagggat gatatttatc tcaccatcgc
      961 atcgatagcc gatttccagc cctgggtgac aggaggcgtc gtcccccctc ccgcaaaccc
     1021 gctgaccata tcgcaaccct gcatcaggtt gaacgacctt gattccgtag gccggtcagg
     1081 ccggcacctc acgctcttcg agatgatggc ccaccacgca ttcaactccg atgttgagga
     1141 gatatactgg aaggaacgaa ccctggaact ctgcgagcag ttcatctcgt ccatcggcgg
     1201 ggacctctct cgcgtgacct ataaagagca cccctggatc ggtggcggca acgccggccc
     1261 aagcctggaa gtgctgatcg gcggcctcga ggtggcgacg cttgtcttca tgaacctggg
     1321 gcggcagaag accggcaagc ccgggatcga ccttgcaggg gagatgtatt atcccatgtc
     1381 caactggatc gtggatacgg gatacggcct ggaacggttc gtgtgggcgt cccgcggctc
     1441 acccaccata tatgatgccg tcttccccga gatggtcagc agggtgatgg aggccgccgg
     1501 cctcgcccat gcgctcgagg acaaagagta caccaagatc ctctccctga acgcaaaatt
     1561 cgcgggggtc atggatatct cggggaccaa cctttaccag ctccgaaaga aggtcgccac
     1621 cgcgattgaa gtcccgatcg agcggctcga caagatgatc acccccatcg agaaggtgta
     1681 tgcaatcgtc gaccataccc gctgcctcgc ctacatgctc ggcgactgca tcgtgccttc
     1741 gaacgtgaga gaagggtacc tggtgcggct ggtgctccgg aggacgctca ggatgatgaa
     1801 tgaccttggg ataaaagagc ccctggcgga cctcatcgag cagcagatgc gaatcgtcgg
     1861 aacctcctcg tttgagcagg acacgctcat cgtgcgtgag atagtggagc gtgaagttga
     1921 gaagagcaat gccacgatgg agcgcggcac gaagatcgtg cagcggatcg cccgcagtta
     1981 caagaacaag cgggagagag tccccttaaa agaggtgatc accctctacg attcgcacgg
     2041 aatcccgccc gagatagtga gggaagtcgc gctggccgag ggtgccgtgg ttgaccttcc
     2101 ggacaacttt tactcgctga ttgcggacat gcactccgaa tcagggcctg ttgcagagga
     2161 ggatccgttc gcccgttacc gcgatcgtat ctctgccctc cctgcaacaa ggaagctctt
     2221 ctacgagcag ccctgcgaca tggaattcga tgccatggtc ctagacttct ttgacgggta
     2281 cgtggtcatg gaccagacgc tcttctaccc cgaggggggt ggacaacccc ctgactgcgg
     2341 catgctgatg accgcagaga acatggtgcg ggttgacgac gtgaaaaaag tgggcgatgt
     2401 gatcgtccat cacgtcacag gcgggaccct gcggaggggc gaccgcgtga aaggtatcgt
     2461 ggacgaagag cgccgctggt ccctgatgcg ccaccacacc gcgacccact tgctcctcca
     2521 tgcttcaaag gaggtcctcg gtgcgcacat ccaccaggca ggagcgcaga agggcctgga
     2581 gagctcgagg ctcgatatcc ggcactacaa gcacatcact cctgatgagt tgaagaagat
     2641 cgagatcgcc gccaaccgca tggtcatggc cgaccaggtg gtcgacgtca agcaccttga
     2701 ccgcacccgt gccgagcagc tctacgggtt tgccctttac cagggtggcg ttccacccgg
     2761 gcgggagatt cgcatcgtgc aggtcgcagg ggatgtcgaa gcgtgtgctg gcactcactg
     2821 ccgcagcacc ggcgatatcg gctttgtcaa ggtgatccgg gtggagcata tccaggacgg
     2881 gatcgaacgc ctggagtttg ctgcaggcct ggccgccgtg gaatacgtcc atcgcattga
     2941 gtcactggta gcccaggctg cagacgtcct ttctgtccag aacgagaacc tgccggcaac
     3001 cgtcagccgg ttcttctctg agtggaaggg ccagaagaag gagatcgaac gtctccgcca
     3061 gaatctcgtc gaactggaga ttaaaaacct cactctcgac acagtgaaca gcacaccgct
     3121 tcttgtgagg cagatcgacc tctccggcaa ggaactggct gcgatcgggg gggagatctc
     3181 cggaaaggga ggagtggcgc tcctggcaaa caggtcggag ccggccaccg tgctcctgat
     3241 gtcgggcacg gagaaggtga atgcaggaga gatcatcggc caggtctgct cgctccttgg
     3301 cggaaaaggt ggaggttcaa ggacaatggc acagggggga ggccccgacg tgcagcaggt
     3361 cgaccttgcc ctcaaagtgg gcagggaacg gattctcgag gcgctcaggg gataaaggcg
     3421 aaggggagat gcgttgttga ccgacactct tcacgatgat gcaggggata ggggggccgg
     3481 cgctccggcg ggagaggtgg tcgtgctcca gccaggagac gagcgggccc agaagatagg
     3541 gaaggcaatt gcaagccagg ctgcgaacga tatcctgcat gcactccagg agaggccaaa
     3601 gactgcgggc gaccttgcgt cgaccctcgc catgccaatg ggaaccgtga agtatcacat
     3661 cgagaacctc ctcgatgcag gcctcctcga gatacgcgag acacgttaca gcgtgaaggg
     3721 gagggagata aaagtctatg ggttaaagga ccagctcctt atcgtggcgc cgagggtcca
     3781 gaacatcagg tctctcctgc tcaagtacgc aaccctcttc ggcattatta tccttgcctc
     3841 tgtggcggct tacgcgatgc tgcctatcct ttctgctcca ccagggatgg gcggagaaga
     3901 cgttgcaaag ttccagggag agaggtcggt cgggatcatg gccgagcccg ctccctctgc
     3961 ggttgccccc gtccagggac tggccctcga cccagtcctt gcattcttcc ttggcggatg
     4021 cctggtgatc atcctcctca tcatttacga agtggcggtg tggaggaggc tgaaatgagg
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accgcgtcac gggcatcact
     4201 ggcaccagga tgaaaaaatg agattgccct gttatatcct ccggaccctc cgtgcaatca
     4261 gccctgctcc gataagcgcg gcacagcacc cggcaacggg gaatggcgtc ctggtgggaa
     4321 tcgcgctctc cgtgggagac gcggtcgccc cgggagtgac aacctcctct gtcccgggcg
     4381 gtggggcatt gaccatgaac ttcacggtcg tcatgaaccc cttgacatca tagaaatcca
     4441 cgtaataatt gcccctctct gatacgggga acattttcat gaaatacccc tcgtccctcc
     4501 ctgtccgggt agtggtgcgg atatcctgtg gggggagcac ggcaccctgc gggccgctga
     4561 ctttcagggt aatggtggtc acgcctgcct tcgggatata cccttccacc cggagcgctt
     4621 ctcccagcac ctggtcggtg gggacagtca gcacgatctc cccggacctg tcagtaatcc
     4681 ggaataccct caatgtgacc gagtccgacc cgagtttcga cccgggatcc gtcaggaacc
     4741 tggcctcgac cttgtagtct cccgcctcca tgccagtcgt gggaaaagag gcctggaatg
     4801 attttgactc gtcgacgatg atgatcgttc tcgtgacctc cgccggggtc gtcggttgca
     4861 ggcgatagat caccaggtca aactgggttc ccaccggaaa agtggtgttt cccacgacct
     4921 cgaggggtgc cccggccctg atctctgatg gggccgatat cgtgagcacg tacgcattca
     4981 cgctccccgc aagtacgaac aggccgagca ggaaaaaaac cgctgatctc atgtcatgct
     5041 ctcagtgggc aggagataat tagtttctgc tgaacgcgac atcgccgacg cactggccgc
     5101 gatgccccga tgagccgcac tgtggaaggc agtgcaggaa gaaattcaaa aaaccgaaaa
     5161 ttgccactca ttttttattt ggttacttct tttcatcaat ttcacagttc gttcagaata
     5221 aaatatataa attgtcagca catgtttctc tcatatggat tctgtgaaac tgaaggttca
     5281 ggaacgtgct tttccgagca ggggacgtgc caggttgcac aaatcatacc tttcacaact
     5341 tgggatcaaa gaaggggacg atgtggagat ctccctcggg gaagaggcaa aacctgtggt
     5401 tgttgcggtc tttgccgaca ccctggttga ggaagggcat atccgtctca gccccgagga
     5461 tattgccgcc atcggtgcag ccccagggtc gagcgtgatt gtgacgagga gaccgcccct
     5521 ggctgaccgg gtaaaaacag gggcagaaac gacggcggaa aaggtgaagg ggggtgcagc
     5581 acaggctgca gagaagatca ggggaggagc ccctgaggct gccgggaagg tgaaggagag
     5641 cgcaacccag gcagcagaga aagtcaagga aggagcctca caggctgctg aaaaggtaaa
     5701 agagggagcg agcgttgcaa aagaatcaat cgagaagagc accgagactg tgaagaagaa
     5761 actacaggac aaagacctgt aagcagggga gacaaccgat ggcagatata tgggtgaccg
     5821 tgaaccagtc cttccccctc cttctcatca tccttggcct catcatcgtc tatttcatca
     5881 tccgggaagt ccggatcatg tatacaaaga cgaggattgc gaagatggac cttgagaagg
     5941 agaaactcca gctgatcaag gcagacctcg agcagaaagg gcatcctttc tttcgcgtct
     6001 ctcccgcaaa acaggaggaa cttcggagcc ttgacgagga gaacacgatg ctcgagaccg
     6061 atatcttcgc ccagcagagc gcggtagaga agcggatcca gagactcgag agcaaggtca
     6121 agctgaccaa gctcgaccac atgattgaga agattaaaaa ggaagaggac agactggggt
     6181 gatcacaaat gacggtctat gaaacacccg gagcaattgg tgaaaaacat tcattcattg
     6241 attcgatcaa ggacatccct ggctacattg accagcggct cccgaccatg gacaaggacc
     6301 tcgatgcata cttcgaccag aacctgccgg cgatcatcga ggagtggggc cttcttggca
     6361 gcgtgcacct aaaagaactc gaacggaggc tctcccgcgt gcaccagcag atagatttcc
     6421 tggagaaggg ccggacctcg ctcgaggcca gggcggcaaa acttgaaaag gagctgaaga
     6481 agctggaggg attatgatgg gacttgacta catggagcgg tacatcgacc gccgcatgaa
     6541 ggatctcatc gaggattgga accttgccac gaggaacgac ctcggagacc tctactcgag
     6601 gctccatgca atcgaagacg aggcgaggat gtgcgccggg ttcgaagcca ccgccactgc
     6661 caagatgaca gagcttgaga aacgcctgga acacctgaag ggggcaaaga gatgacagtg
     6721 accgaagctc tccttatatt cctgctgatt atcctcatcg gcttcatcat ctactactac
     6781 ttcagggggt catccggaaa actcgagatc acccggccga tggagagcag gctcgatgag
     6841 tatctcgaca ggcgcttcga ttcgatgatc gatgaatggt ctctgctgcg caagccccag
     6901 gcacagaagt tccgggacga gaagcttgcc gacctctccc gggacgagaa acggatggct
     6961 tcgctttcag agtttgagag acagttcggg gagcagatgg ccaacctgga ggagcggctt
     7021 gaaactctgg aaaagcaggt catcaggtaa caaacggcgc gagagcgaga aggaagacct
     7081 ggtctcacgg ctgaaaaaag acgtcccaat gatgagaggc cagggggagc cccccgacaa
     7141 ggacccggca cacttctcgg ggttcctctg cgatctctgc aacagcccgt tcgcctatca
     7201 tgaactcagg cagtgcatcc tgtgcgggag atgggcatgc tcttcctgct ggacacctga
     7261 attttacgtc tgcaactcgt gcaacgggat gatcacgctg cacaccatgg gggataagag
     7321 ttccaggtga cgcgtgccgg gagttgaggg caacccgcgc atatcttttt tcagagggat
     7381 tttttattcc cgcaatggta ttgtcgcctc caattaattt tggtccacgg ttttttcctc
     7441 cctgaaggga tccccgatac attcatagcc atggcctgtg ccatagttca ctatggctga
     7501 tactgtaatc cttgtcactc catgtgacag gggatggagt gccctggtca tcctcgggct
     7561 cctctcgata ctctttggag
//
LOCUS       DAOQVJ010000008         7343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_11140_length_7343_cov_5.259742, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000008 DAOQVJ010000000
VERSION     DAOQVJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11140_length_7343_cov_5.259742"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKL26_00365"
     CDS             <1..362
                     /locus_tag="PKL26_00365"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNS82179.1"
                     /translation="GGCRSMPSLCRFDPGRGAALYAEVDGALAALARSLRERFRIDRI
                     LLFGSYARRDVNEGSDIDLLLVGDFPLRFHERPSLIRDLTDLPVEPLCYTPAEFAELI
                     AEGNPFITEVLSEGREL"
     gene            complement(515..949)
                     /locus_tag="PKL26_00370"
     CDS             complement(515..949)
                     /locus_tag="PKL26_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNS82180.1"
                     /translation="MTATPPLVDTNILVYLFDRDEPDKRTIARGIVEGCFSGHDQLAV
                     SVQNLAEFAVVMTEKVEHPMPREMVSRFIRDIASFSQWWVLGYDAACIEDAVAIGGER
                     RIHFWDALLVATMKRHGITTLYSEDEHFIKVPDIRVVNPFIR"
     gene            complement(924..1178)
                     /locus_tag="PKL26_00375"
     CDS             complement(924..1178)
                     /locus_tag="PKL26_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82181.1"
                     /translation="MAVITISIDDEIEQRFREVAKRVIGEKKGYIRQAVSEALQLWLD
                     QKDQERIATDALAIIGSGYHLGEKRYKKREDLYDRNSSTR"
     gene            complement(1867..2832)
                     /locus_tag="PKL26_00380"
     CDS             complement(1867..2832)
                     /locus_tag="PKL26_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82182.1"
                     /translation="MVLEQKVLFAEGEDANAFVAFLRKAGCPSQKVVQGVAAREEILS
                     GTLDQAISWLAGEKEHCEGEGNTEDASWFRTVEESYRQAHAYASGLLQGKQAGDLVFT
                     KKEMMSELMELIKSFADGEEEEGQEEAPGSSGENAIRSLVLAMLRVSNAVRETEEGFC
                     LDRPITAGELPVKFNLEKFPPIDPERTGDLMMQMIYRISRIHMVTADPLIHIACDPDQ
                     VMDLLCTLSVPEEVFDAVTEELAIKGLAVSYIITEVEEKKSTSVSALTQGLNELGISA
                     KEITVPIQVHVDEETVAAIVAELRKRDILAGTDEKVRMAGGKRRR"
     gene            complement(3112..3522)
                     /locus_tag="PKL26_00385"
     CDS             complement(3112..3522)
                     /locus_tag="PKL26_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNS82183.1"
                     /translation="MTVIDSSAYLAFLIGEEGREQVRPWLDPAREPRSLSLLLAETGN
                     VLWKYTRQGHLRAEEARTLFSHTLDLCREGVIGIEPIERYGDQALRLAIEYHHPLYDM
                     LFVAQAIETNETLVTGDRLQAEIGSRCGVEVVRI"
     gene            complement(3509..3856)
                     /locus_tag="PKL26_00390"
     CDS             complement(3509..3856)
                     /locus_tag="PKL26_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82184.1"
                     /translation="MYLLPIRDTAHDEKVGDIFTEKRASVVQKLKELYYIMRYMCPTL
                     SVRLPDQLKEGMDRLRDRVDWCDEIRRFIEKKIEDERKRAVLRDLETQITQLPKSPRG
                     TSSRLVRGDRDSH"
     gene            4103..4699
                     /locus_tag="PKL26_00395"
     CDS             4103..4699
                     /locus_tag="PKL26_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS82185.1"
                     /translation="MYPLAHLLAGILLGILLAVLARDSRLIPACALGSILPDLVDKPV
                     GLLLFPAIFGTGRIFFHALLIFGLILFTGILVYTKYPHTGILVLAVAAGVITHQVLDA
                     MWLQPSNWYWPALGPFKGQSRPDFFIDAFWRTVKNPTEWLAGIAILAFLVIAAVPRYG
                     ERFRSDLKGPWIRRLSLPLILLLSATAVLLVAIGIYYG"
     gene            4968..6092
                     /locus_tag="PKL26_00400"
     CDS             4968..6092
                     /locus_tag="PKL26_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS82186.1"
                     /translation="MNGPKKSREHESGNEDVNISILSLINHYTAFSETIKGNSATTRA
                     HNAISMNNTHPSYPKDPIISIPNNIPELLNKNPSKQFTIAAMPAYNEEKYIAKTIINA
                     RKYVDKVLVVDDYSSDSTNEIATALGAIVVRHPENRGYGGALQTIFNKAREVHADKLV
                     ILDSDGQHDPQFIPELLKPLDAGYDLVIGSRFLSTDEKGIPSYRIVGMKVLDTATKFA
                     GEINISDSQSGFRAYGKKAIECIKVSGNGMSAGSEILLQIKDHDLRFTEVPIKVRYDL
                     EDTSSQNPVIHGVSVLNAIVGMLGYRRPLLSFGIPGICITAIGIIAGSWAFSAYYTNH
                     MLPFGPTFLSGIAIMAGLLLITTALILNSLVQIVKMERGS"
     gene            6094..7158
                     /gene="wecB"
                     /locus_tag="PKL26_00405"
     CDS             6094..7158
                     /gene="wecB"
                     /locus_tag="PKL26_00405"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNS82187.1"
                     /translation="MIAIVLGTRPEIIKMSPIIRECEKSSIPYYILHTGQHYSYDMDR
                     VFFEELELPGAEFNLDVGSGSHAEQTGKILLGVEKILNSKPTDVVLVQGDTNTVMAAA
                     LAAAKLNIPVGHVEAGLRSFDRRMPEETNRVVADHISTYLFAPTNTAKDHLLHEGIPK
                     EKIHVTGNTIVDAVYQNVEIGRKKGDILSELGLDPGEYFLVTAHRAENVDQKSRLEGI
                     FQGLQEVQKEYEIPVVFPIHPRTQKMLSLFGIAHDGIRTVPPVGFLPFLQLESNARLV
                     LTDSGGVQEETCIIGVPCVTLRENTERPETLEVGANVLAGTDANSIKNAVHSMLDSPV
                     TWTNPFGDGDAGKRITRICS"
     gene            7196..>7343
                     /locus_tag="PKL26_00410"
     CDS             7196..>7343
                     /locus_tag="PKL26_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS82188.1"
                     /translation="MQTEKILVTGGAGFIGTNLVNELASRGHEVLAVDLYNNEREQYI
                     RADVK"
BASE COUNT         1685 a         2030 c         1805 g         1823 t
ORIGIN      
        1 aaggaggatg ccgatcgatg ccttcactct gcaggtttga tcctggacgc ggtgcagcgc
       61 tgtatgccga ggtagatggg gcactggcgg ctcttgcccg atcgctccga gaacgattcc
      121 ggatcgaccg catcctcctc tttggttcct atgccagaag ggacgtaaac gaggggagtg
      181 atatcgatct ccttctagta ggggactttc cgctccggtt ccatgaacgg ccgtctctta
      241 tccgggacct gactgatctc cccgttgagc cgctctgtta cacccctgcc gagtttgccg
      301 aattaatcgc cgagggcaat ccgttcatca ccgaagtgct gagtgaagga cgagaactct
      361 aaccttctct ccggatcatg aatctctgat tctttgtcaa tcgtgctgta ctgaaagaaa
      421 gagcattacc ccatcttaga acaacgccca gggtactcat cccatccatg tgggggggtg
      481 gcggggggac ggatggggag tgaggaatcg ttcctcacct gataaacggg tttaccaccc
      541 tgatatcagg gacttttatg aaatgttcat cctcagaata gagagtggtg ataccgtgcc
      601 gtttcatcgt ggcaacaaga agggcatccc agaagtggat tcttcgctct cccccgattg
      661 ccactgcatc ttcgatgcac gcggcgtcat aaccgagcac ccaccattgg gagaatgatg
      721 caatatccct gatgaaccgc gagaccatct cccgaggcat ggggtgctcg accttttcgg
      781 tcatgaccac ggcaaactcg gcaagattct ggaccgagac ggcaagctgg tcgtggccgg
      841 aaaagcaccc ttccacgatt ccccgtgcaa tcgttctctt gtccggctca tcacgatcga
      901 agaggtacac gaggatgttg gtatcaacga gtggaggagt tgcggtcata cagatcctcc
      961 cgtttcttgt atcgtttctc tcccaggtga tacccgctcc cgataatggc cagagcatcg
     1021 gtagcaatcc gttcctggtc tttctggtcc agccagagct ggagcgcttc agataccgct
     1081 tggcggatat accctttctt ctccccgatc acccgctttg ccacctccct gaaccgttgc
     1141 tcgatctcgt catcaatgct gatcgtaatg actgccatag atatccatac aattctcaca
     1201 tatttaaatg ttttattaat ttaaatacac tgattcgata ggcattcacg aggccgattg
     1261 agctgggaaa agttaggtta atggaaacga atcatgtccc catattcaaa aaaaagctta
     1321 aaaacgacgc atcagaaaat gtatctgctc ccaatagaga ttcccgcacc acccttcgtc
     1381 atcgtcaggg ggggttgggg gcacagatac catgctggat tggggcggtt ctcgttccct
     1441 tcaatactcc gagagggtaa gaccaaagag gatactattg cttcgcctca aaggtagtga
     1501 tgattgacct ccggtctttc tcgagaggga actcctccaa gtataattct gtggcttcct
     1561 tgaggttatt caccgcttcc tcaatggtat atccctggct caccgtacct acttccgggc
     1621 acagagcgac atacctgtca tcctctttgt gtagtactgc agtgaacgta gacatggcct
     1681 tgtcatacca ctactctctt ttctcatacc atactttctt ccaatagagg acgaggatat
     1741 gatactccgc atctcctcat cccttcgctc acaggggtgg gtgggggggg ggagggaggg
     1801 ggggcggtag tgatattcga attgttccct tgaatccact ccggcgggta ctctgacctc
     1861 tgccggttac ctcctgcgct tcccccctgc catgcgaact ttctcatccg ttcccgccag
     1921 gatatcccgc ttccggagct ctgccacgat cgcggcaacc gtctcttcgt ccacatgtac
     1981 ctggatcggg accgtgatct ctttcgccga aatacctagc tcgttgagcc cctgggtcag
     2041 ggcactcacc gatgtactct tcttctcttc cacttccgtg atgatatagg agaccgcgag
     2101 ccccttgata gcaagttcct ccgtgacggc gtcaaatacc tcttcgggga ccgagagcgt
     2161 gcacaggagg tccatgacct ggtcgggatc acaggcgata tggatgaggg gatcggcggt
     2221 taccatatgg atccgactga tccggtagat catctgcatc atgaggtccc cggtccgttc
     2281 cggatcgatg ggcgggaact tctccaggtt gaacttcacc gggagctccc ctgccgtgat
     2341 cgggcgatca aggcagaacc cttcctcggt ctccctcact gcgttgctca cccggagcat
     2401 ggcaaggaca agggaccgga tggcgttctc cccggaagat ccgggagcct cttcctgccc
     2461 ctcctcctct tcaccgtccg cgaacgactt gatcaactcc atgagctcgc tcatcatctc
     2521 cttcttggtg aagaccaggt ctcccgcctg cttcccctgg aggaggcctg atgcgtacgc
     2581 atgggcctgc cggtagctct cttccacggt ccggaaccag gacgcatctt cggtgttccc
     2641 ttccccctcg cagtgctcct tctcaccggc aagccaggag atcgcctggt ccagggtgcc
     2701 cgagaggatc tcctcccttg cagcaacccc ctggaccacc ttctggctgg ggcaccccgc
     2761 cttccgcagg aacgccacaa atgcgttggc atcctccccc tccgcaaaca ataccttctg
     2821 ttcaagcacc attgatgcag ctatggggcg tgccagatca aaaatacggt gggttgtatc
     2881 cctactcaag tcatggagaa gaggtacgta atcgtcgagg ttcaggagat cttccctgag
     2941 tagagaatgt accgcctcag catactatcc cgctcactgg ggtgggagat gggggagggg
     3001 tggaggagga tcgcatctgt caacaccaat ggagcactcc cctcccgtag gtaaggagtc
     3061 tctcccaggc ggatactctg acctatgccc tggcgccatc tatcgatccc atcatatcct
     3121 gacgacctct actccacatc gggatccaat ctcagcctgt aatcggtccc cggtcaccag
     3181 tgtctcattt gtctcaatgg cctgggcaac aaagagcata tcgtacaagg gatggtgata
     3241 ttctatggcg agcctgagtg cctgatcgcc gtagcgctca atgggctcga taccgatcac
     3301 cccctccctg cagagatcga gggtatgaga aaagagggta cgtgcttctt ccgcccggag
     3361 gtgtccctgc cgggtatatt tccagagcac attgccggtc tcggcaagaa gcagggagag
     3421 cgaccggggt tcccgtgcag ggtccagcca aggccggacc tgttctcttc cctcctctcc
     3481 tatcaggaac gcgaggtagg ccgagctgtc aatgactgtc acgatcccct ctcaccagcc
     3541 gtgatgaagt cccccggggg gatttgggga gctgtgtgat ctgcgtctcc agatcccgga
     3601 gcacggccct cttgcgctca tcctcgatct tcttctcgat gaatcgccgg atctcatcgc
     3661 accagtctac ccgatctctc aggcggtcca tcccttcctt cagctgatcc gggagtctca
     3721 cgctgagtgt aggacacata tatctcatta tgtaatacag ctctttaagc ttctgtacta
     3781 cagatgccct cttttctgtg aaaatgtctc caactttttc atcgtgtgcc gtatccctta
     3841 tcggaagcag gtacaatttt tcccggggga gagaaaggct ctggtcccgg gcattcctga
     3901 aacaggattc aggagcggta tggcgagcag agaatcgatg ccaggattcc tcgcatatcg
     3961 gggtgggtgg cggggggatg gatggtgggt aagtcaggca ccacccctgc tgaattcccc
     4021 caaaaccccc agcagcacac tccctcttct gtaatgtccc gcccgaacct tcatacccct
     4081 tcccgcataa cgctccctgc caatgtatcc cctcgcccac ctgcttgccg gcatcctcct
     4141 cgggatcctc ctcgccgttc tcgcccggga ctcccgtctc atcccggcct gtgcccttgg
     4201 aagcatcctc ccggacctgg tcgacaagcc ggtcgggctc ctcctcttcc ccgcaatctt
     4261 cgggaccggc aggatcttct tccacgccct gctcatcttc ggcctgattc tttttaccgg
     4321 gattctcgtc tatacgaagt acccgcatac tggcatcctg gtccttgccg ttgccgccgg
     4381 cgtcatcacc caccaggtcc ttgacgccat gtggctccag ccatcgaact ggtactggcc
     4441 ggcactcggc ccgttcaaag gccagtcccg gcccgacttc ttcatcgacg ccttctggag
     4501 aacggtcaaa aatccgaccg aatggcttgc ggggatcgcg atcctggcat tcctggtcat
     4561 tgctgccgtt ccccgctacg gggagcgatt tcggtccgat ctgaagggac cgtggatcag
     4621 gcggctctca cttccgctca tccttctcct ttcggcaaca gcagtcctgc tggtggcgat
     4681 cgggatctat tacgggtaac ccggcgaatc cctcacagca gagagcagga attcaccctg
     4741 ttcccgatcc accctggagc agtcctcgct gcggttaatc ctcctgattt cgattatcca
     4801 attagattaa ccaaatctga aacttttttg gaaacgtttt tctttcctta atcctcaata
     4861 atttatagta cgacacagga aggcattggg gatgcctttc ttttaccttg ggggactgca
     4921 atacgcatac caatcaatac tcggaatcct acacggacca ggattctttg aacggtccga
     4981 aaaaatccag agaacatgag agcggtaacg aggatgtgaa tatttccatt ctctcactca
     5041 tcaaccacta cactgctttc agtgagacga tcaagggaaa ttcggcaaca acaagagctc
     5101 ataatgcgat ttcgatgaat aacactcatc caagttatcc gaaagacccc ataatttcta
     5161 tccccaacaa tattcctgaa ttactgaaca agaatccctc aaaacaattt actattgccg
     5221 caatgccggc ttataatgaa gagaaatata tcgctaaaac aatcatcaat gcaagaaaat
     5281 acgtcgataa agtccttgtt gtagatgact attcatctga ttcaacaaat gaaatcgcaa
     5341 cagctcttgg agctattgta gtccgacacc cagaaaatcg agggtatggg ggagctcttc
     5401 agaccatatt caataaagca cgtgaagtac atgcggataa attagtgatt cttgattcag
     5461 atggtcagca tgatccccaa ttcatccctg aacttctaaa accccttgat gctggatatg
     5521 accttgtaat tggatcgcgt tttctttcaa ccgatgaaaa aggaattcct tcttaccgta
     5581 ttgtcgggat gaaggtgctg gatactgcaa caaaatttgc aggggagatc aatatttcag
     5641 attcgcagag cggttttcga gcttacggca agaaggcaat tgaatgcatc aaggtgagcg
     5701 ggaacgggat gtctgccgga tctgaaatat tgctccagat caaagatcat gatttacggt
     5761 tcactgaagt tccaataaaa gtccgatatg acctcgaaga tacttcgagc cagaatccgg
     5821 tcattcatgg ggtatctgtt ctgaatgcca tcgtaggcat gttgggctat cgaagaccgt
     5881 tattaagttt cgggattccc ggaatttgta ttactgcaat tgggattatt gcaggatcct
     5941 gggctttttc tgcatattat actaaccata tgcttccttt tggcccgaca tttttgagcg
     6001 gcatcgccat tatggccgga ttattactca tcacaactgc attaatcctg aattcactcg
     6061 tgcagattgt gaagatggag agaggaagct gatttgatcg ccatcgtact tgggactcgg
     6121 cctgagatca tcaagatgtc ccctatcatc agggaatgcg aaaaaagcag cattccttac
     6181 tatatcctcc acacgggcca acattattcc tatgacatgg acagggtatt ttttgaggaa
     6241 cttgaattac caggtgctga gttcaatctc gatgtgggat cgggctccca tgcagaacag
     6301 acaggaaaga tactcctggg agttgagaag atcctcaatt cgaaacccac cgatgttgtc
     6361 cttgtccagg gggataccaa tacggtcatg gcagcagccc ttgccgccgc caaactcaat
     6421 atcccggttg gccatgttga ggcaggtctc cgttcatttg accggcgtat gcctgaagag
     6481 accaacaggg tagttgccga tcacatctcg acatatctat ttgctccaac caatactgca
     6541 aaagatcatc tcctccatga aggaattcct aaagagaaga tccatgtaac cggcaatacc
     6601 atcgtcgatg cagtatatca gaatgttgag atagggcgga agaaaggaga tattctctcg
     6661 gaactcgggc tcgatcccgg tgaatatttc cttgtgaccg ctcaccgggc tgagaatgta
     6721 gatcagaagt ctcgtcttga agggattttc caaggactcc aggaagtcca gaaagagtat
     6781 gaaatcccgg ttgttttccc tatccatccc cggacccaga agatgctgtc cctcttcgga
     6841 atcgctcacg atggaattcg gacggttccc cctgtaggat ttctcccatt cctccagctt
     6901 gaatcgaatg cccgtcttgt tctcaccgat tctggtggcg tacaggaaga gacctgtatc
     6961 attggagttc cctgtgttac gttacgtgaa aataccgagc gtcccgaaac tcttgaagtt
     7021 ggagcaaatg tattagccgg gactgatgcc aactcgatta aaaatgcagt tcattctatg
     7081 ctggattcgc cggttacgtg gactaatcct ttcggggacg gggatgcagg aaagagaatc
     7141 acgaggatat gttcgtgaag gattacctca gaatgtatgt tttaaggaga tactaatgca
     7201 gacagaaaaa attctggtga ccgggggtgc aggcttcatc gggaccaacc tcgtgaatga
     7261 acttgcttcc aggggtcatg aagtacttgc agttgatctt tacaataatg agcgggaaca
     7321 gtacatccgt gcagatgtga aga
//
LOCUS       DAOQVJ010000009         7290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_11248_length_7290_cov_4.327160, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000009 DAOQVJ010000000
VERSION     DAOQVJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11248_length_7290_cov_4.327160"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1028
                     /gene="ilvE"
                     /locus_tag="PKL26_00415"
     CDS             162..1028
                     /gene="ilvE"
                     /locus_tag="PKL26_00415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNS82189.1"
                     /translation="MFVYIDGKFYPKEEARISVFDHGLLYGDGVFEGIRAYNGRVFRL
                     QEHLDRLYDSAKTIDLCVPLSKEEMTSAIIETLRKNNLKEAYIRPIVTRGMGDLGLCP
                     TKCPKATVIIIAVEWGAMYGDLYEKGLKAVTVSVRRNPCCSLPPNVKSLNYLNNILAK
                     IEANCKGGDEAIMFDMQGHISEGSGDNIYIIKNGVILTPPTLNNLRGITRMVVLEIAG
                     QLGIPVQETNLGYFDLYTADEVFVTGTAAEVAPIVFIDGRSIGSGRPGPITRQLMAAF
                     KTVTEREGTPVY"
     gene            1098..1172
                     /locus_tag="PKL26_00420"
     tRNA            1098..1172
                     /locus_tag="PKL26_00420"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Glu,seq:ttc)
     gene            1335..2981
                     /locus_tag="PKL26_00425"
     CDS             1335..2981
                     /locus_tag="PKL26_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82190.1"
                     /translation="MAVALATFTILVLTWIALHSGIAIIYQNAYYIPIILSCYFFQRR
                     GLYFSIGLICIYLLTLVWAFPTIEIILQGLVRVLFFLLIALLVTYLSETIQREKSRYK
                     GIFSSSESAIIIFRKAPLEIIEANPQCTEITGYSGEELVGSNVADLFLDKDRLAPSFA
                     GVDGPFTHHETVLMRKDGDLKTVLLSTGSAEHGIGTVTMQDISLRKQAEETIKASEEK
                     FRMLFEHSGDPIMILGMDGSILEVNSAAVKMLGYTRDELTKMNARDIDTPDNTALFEK
                     RMRELHTSGRILFETAHRTTSGEAIPIEVHATLIRFQGKPSILSTPRDISERKRAEIA
                     IQQANRKLNLLNSITRHDILNSLTALMGYLELSLEDTKDPQMREYIHAEMRAAQTIRK
                     QIEFTRDYQDVGVKSPGWFSVPVMIRQMRSRIEDSKARIEIDLPNFEIFADPLVEKVF
                     YNLMENAIRHGEHTTRISFTSEECDGNLVIACSDDGPGVPLDKKKAIFNREYFKHSGF
                     GLFLSREILGITGIQIQENGVPGKGARFELIIPPEGFRYP"
     gene            3050..>3681
                     /locus_tag="PKL26_00430"
     CDS             3050..>3681
                     /locus_tag="PKL26_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82191.1"
                     /translation="MILIETHVYKMKIADAIFTLKKGARDFPMAPNSGPGNDAARCEK
                     SIPARADGRLPAWRIRSRNNFSLKHAYHTDTWLNHLTGAWQAPSCQRNRFKKRPLPIS
                     SRIQSERVWSARQAGELHGRQIMEEKRGKFVKGAWIEEEQVSAKPGDEVKTEPIDQRI
                     DRVTSVFTTSFQQVIGLARDLVTTPEGHAHIEKQVNQAVTQIERAISDVL"
     assembly_gap    3682..3781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3940..4683
                     /locus_tag="PKL26_00435"
     CDS             3940..4683
                     /locus_tag="PKL26_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNS82192.1"
                     /translation="MPAELKRDPMENTWQERLIAFLRQPEIGYADPGTDAIARYSAAF
                     THQSYIREAQCGEESPPLYEDYERLEFLGDRVLNLIVAEYLFSLGHHSEGSMTSCMEV
                     VKNQHIGAIVPSLGIGFTEMIRVGRNQQKTTRIIAGSFEAFIGAHYLERGLESTRRMI
                     MRLIGDDIAAFSPGDNYKKILQEELQATLGTLPVYVLHEKKGPDHRPEFVYRVLIGRR
                     VAGEGKGRNKAEATQNAALDALRARGSLS"
     gene            4855..5277
                     /locus_tag="PKL26_00440"
     CDS             4855..5277
                     /locus_tag="PKL26_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS82193.1"
                     /translation="MKKILAAVDGSPLTHNVIMAAVEEARCHGAELHIVHVLENAWAE
                     GDVARELAIRDLEEEAASLLSSMKAEAEAQGGEAVLHLLRGHPGDRIVDCAIDIGADM
                     VVLGSLGRSQVKRMIAGSVSTFVVTHCPITTMVVKPGS"
     gene            5492..6919
                     /locus_tag="PKL26_00445"
     CDS             5492..6919
                     /locus_tag="PKL26_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNS82194.1"
                     /translation="MNDACSGNDGPTLRQGIIQAVVLAAIAMAVIDGIVVSIALPSIT
                     RDFSSDVALSQWIITAYLVTETSLLLIFGRLSEFTGKRTLFLAGLVLFTTSSLACGLA
                     ASLLELIVFRVIQASGSAMIFSISAAILFETSVPGSQGKVMGYIGAVTAAAGLAAPIL
                     GGFITDALGWAFIFLINVPIGALGLVLFARHSPPGERRDTSIRMDWAGSATLVIALLF
                     LVLFLVRVSSALSWSAESMVFLLVSTLSMILFLVAESRSSHPLLDLSIFRKLGFTLPN
                     LAMVCIYMAFFMLNLIGPFYLEGVMDLTPSQVGLVFLIAPLIMVIASPLSGWLYDRYH
                     LFSLPTLGILLTGVSLLLLGFVVVRQDLLLIVLLFIPLSVGISLFHSPSSTDIMRALP
                     IAKSGLASSVSATLRNLGMTLGVSVAGILFTKELYDTGYTGPVQEAAAGVLAGVIGNL
                     LFIAAALCGVGAALYLAKNILIDRV"
     gene            7021..>7290
                     /locus_tag="PKL26_00450"
     CDS             7021..>7290
                     /locus_tag="PKL26_00450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNS82195.1"
                     /translation="MKILYVYAHPEPRSFNGALKDAAMVTLAVKGHDVKVSNLYAVRF
                     KAILDAQDFRIRGRHDIFNPAAEQIHGIRTGSLAPDIQEEIERVSW"
BASE COUNT         1698 a         2016 c         1878 g         1598 t
ORIGIN      
        1 cggacggtgc gctcacgaac aggacaattg ggagctacac ccaccttcat cccgtggcaa
       61 gcctctccat gttctcgcat ttcctctcga cctgccggat gcaggtcaac gcctgattca
      121 atcagcaggt aaaaaatacc aggagaataa acattcattt catgttcgtc tatatcgatg
      181 ggaagttcta tcccaaagag gaggccagga tctctgtctt tgaccatggc ctcctctatg
      241 gagacggggt gttcgagggg ataagggcat acaacggccg ggtgttccgc ctgcaggagc
      301 accttgaccg cctgtacgat tcggcgaaga ccatcgacct ctgtgtcccc ctctcaaaag
      361 aggagatgac cagcgcgatc atcgagacgc tccgtaagaa caatctgaag gaggcgtata
      421 tccgacccat cgtgacccgt ggcatgggag atcttggcct ctgccccacc aaatgcccaa
      481 aagcgacggt catcatcatc gcagtggaat ggggagccat gtatggggac ctctatgaga
      541 agggactaaa agctgtcacc gtctcggtga ggcgtaaccc ctgctgttcc ctgccgccga
      601 acgtaaagtc cctcaattac ttgaataaca tccttgcaaa gatcgaggca aactgcaagg
      661 gaggagacga ggccatcatg ttcgacatgc aggggcacat ctccgaaggg tccggagata
      721 atatctatat catcaagaac ggagtcatcc tgacacctcc caccctgaat aacctgagag
      781 gaatcacccg gatggtggtg ctggagatcg ccgggcagct cgggattcct gtgcaggaga
      841 caaaccttgg ctatttcgat ctttacaccg cagacgaggt ctttgtcacc ggcaccgcgg
      901 cagaggtggc gcccattgtc tttatcgacg ggaggagcat cggttcaggc cgcccgggcc
      961 ccatcacccg gcagttgatg gctgcgttca agaccgtcac cgagcgagag ggcacccccg
     1021 tctattgaga aaaactcccc ccatctaccg gctccacaac cctttttacc gtgaaccgcc
     1081 aactaaaaag agcaattgct gctatagtgt agtccggcca atcatgccgg cctttcacgc
     1141 cggcgactgg ggttcgaatc cccatagcag cactgttttt cacatttttc ccccccttta
     1201 acttagccat atcctgtttt attagagaaa acgacgaata tttttttata ataatcattc
     1261 caacacccgt aggatccccc ttcatgaaga taccaagccg gaattctcag cctggatcta
     1321 cccgcatcca tctcatggcc gtcgcattgg caacgttcac catcctggtc ttgacatgga
     1381 ttgctcttca ttccggtatc gctatcatct accagaatgc ctattatatc cccataatcc
     1441 tctcctgcta tttttttcaa cggagagggc tttatttctc catcggcctc atctgcatct
     1501 atctcctgac gctcgtatgg gccttcccca caattgaaat catactccag ggactggtcc
     1561 gggtgctctt cttcctcctc attgcattgc tggtaaccta tctctctgag accatccaga
     1621 gagagaagtc aaggtacaag ggtatcttct cctcctctga atcggccatc atcattttca
     1681 ggaaagcccc ccttgaaatc atcgaggcaa acccgcaatg cactgagatt accggttatt
     1741 ccggtgaaga actggtcggt tccaacgtcg ctgacctttt cttagacaaa gaccgcctgg
     1801 caccatcctt cgctggagtg gatgggcctt tcacgcacca tgaaacagtg ctcatgcgta
     1861 aagacggaga cttgaagacc gtgctcctct cgacaggctc tgcagaacat ggaatcggca
     1921 ctgtcaccat gcaggacatc tcgcttcgca aacaggctga agaaaccata aaagcaagtg
     1981 aagagaagtt ccgtatgctc tttgaacaca gcggggaccc tatcatgatc cttggcatgg
     2041 acggctcgat tcttgaggta aacagcgcag cagtgaagat gctcggctac acgcgtgacg
     2101 aattgacgaa aatgaatgcc cgcgatattg atacccccga caacacggcc ctgtttgaga
     2161 agcggatgcg agaactgcat acatccggaa gaatcctctt cgagactgcc caccggacga
     2221 catccgggga ggcaatccca atcgaagtgc atgccacgct tatcaggttc cagggcaaac
     2281 cttcaatcct cagcaccccc cgggacatat cagagcgaaa aagggcagag attgccatcc
     2341 agcaggcaaa caggaaactg aacctcttaa acagtattac acgccacgac atcctcaatt
     2401 ccctgacggc actcatgggt tacctcgaac tctcgctgga agatacgaaa gatccccaaa
     2461 tgagagagta tattcacgcg gagatgcggg cggcgcagac catccggaag caaatcgagt
     2521 tcacgaggga ttaccaggac gtaggtgtga aatctcccgg gtggttctct gtccctgtca
     2581 tgatccggca aatgagaagc cgcattgaag attcaaaagc gcggatcgag atagaccttc
     2641 ccaatttcga gatattcgcg gatccgcttg tcgaaaaggt cttctacaat ctcatggaga
     2701 atgccatccg ccatggagaa cacaccacgc ggatcagctt cacttctgaa gagtgcgacg
     2761 gcaatcttgt gattgcctgc tccgatgacg ggccgggagt tccactcgac aaaaagaagg
     2821 ctattttcaa ccgtgagtac ttcaagcatt cggggtttgg gctcttcctc tccagggaga
     2881 tcctgggaat taccggtata cagatccagg agaacggggt tccggggaaa ggggcgcggt
     2941 tcgaactgat aattcccccg gaggggttca ggtatccctg atacccgacg ctgccctcac
     3001 cggactcctg cagcagccgg gccttgaggt gttttcctca aaaaaacaca tgattctcat
     3061 cgagacacac gtatacaaaa tgaaaatcgc ggatgcgatt ttcacattaa aaaagggggc
     3121 aagagacttc cccatggcgc ccaattcagg accagggaac gatgcagcac gctgtgagaa
     3181 atccatccct gcaagagctg atggccggct ccctgcctgg cgcatcagat ccaggaacaa
     3241 tttttctcta aagcacgcat atcacactga tacctggctg aaccatctca cgggggcttg
     3301 gcaggcaccc tcctgccaaa ggaacaggtt caagaagagg cctctcccaa tctcgagcag
     3361 gatacaatca gagcgggtat ggagcgcgag gcaggccggg gaattacacg gcaggcagat
     3421 catggaagag aaacgtggaa aatttgtcaa gggagcatgg atcgaagagg aacaggtgag
     3481 cgcaaaaccg ggagatgagg tgaaaaccga gcccattgat cagagaatcg accgggttac
     3541 ctccgtgttc acgacctctt ttcaacaggt tatcggtctt gcgcgcgacc ttgtcactac
     3601 tccggaaggg catgcgcata tcgaaaagca ggtcaaccag gcagtgacgc agatcgaacg
     3661 tgccatctct gatgtattga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nagcaggtca accaggcagt gacgcagatc gaacgtgcca tctctgatgt attgaacatg
     3841 gaacgcgaag agaagccttc tgaatcatcc gatggtaaga aggagatacg gatcgaatag
     3901 gcaatcaatg gctgcggcga tgaatctttt tcctgatgca tgccggcgga attaaagcgg
     3961 gatccaatgg agaacacctg gcaagaacgg ctcatcgcat tccttcgtca acccgaaatc
     4021 gggtatgccg atcctggaac agatgcaatt gcacggtatt cggcagcatt cacccaccag
     4081 tcgtacataa gggaggcgca atgcggcgaa gaatctcctc ctctctatga ggactatgag
     4141 aggcttgagt ttctcggcga cagagtcctg aacctcatcg ttgcagagta cctcttctcc
     4201 ctggggcatc acagcgaagg gagcatgacc tcgtgcatgg aggttgtgaa gaaccagcac
     4261 attggtgcaa tagtgccctc actcggtatt gggttcacgg agatgatacg tgtggggcgg
     4321 aaccagcaga agacgacaag gatcatcgca ggctcttttg aggcgttcat cggagctcac
     4381 taccttgaga gagggcttga atccacgagg agaatgatca tgcgcctcat tggggacgac
     4441 attgccgcat tctctccagg cgataactac aagaagatcc tgcaggagga gcttcaggcg
     4501 acccttggga cgctccccgt ctacgtcctt catgagaaga agggtccgga ccaccgccct
     4561 gagtttgtat acagggtcct cataggcaga agagtcgctg gcgaaggaaa gggtcgcaac
     4621 aaggccgagg ctacccagaa cgccgccctg gatgcccttc gggcccgtgg aagcctttcc
     4681 tgaaccgaaa ttacgacaag gccataatta catggcctcg aaatggttgc cgtggcatgg
     4741 gagctgcgga gcacttatgt ggtcccatgt ccctagacat acgtatacgg gactgtttcc
     4801 cacgtaagat cgtgcctgga gcagtttctt caccgcaggg ggttgatcag gctgatgaaa
     4861 aagatacttg cggcagtgga cggatccccg ctcactcaca acgtgatcat ggctgcagtt
     4921 gaggaggcaa ggtgccatgg tgcggaactt catattgtcc atgtgctgga gaacgcctgg
     4981 gcagaaggag acgttgcaag ggaactggca atacgtgacc ttgaggagga ggctgcatcc
     5041 ctcctctcat cgatgaaggc agaagcagaa gcacagggtg gcgaggccgt attgcacctt
     5101 ctcaggggtc atcccggaga ccggatcgtt gactgcgcca ttgatattgg tgctgacatg
     5161 gtcgttctcg ggtcattggg caggagccag gtcaagcgga tgatagccgg gagcgtatcc
     5221 acctttgtgg tcacccactg ccccatcacc accatggtgg taaaacccgg ttcctgaatg
     5281 ccgctcccgc gtgaagagca tgaggagaga gtggtctttc ctctgctcct ttgggagaat
     5341 ccgcgccagc ccggcaatta tccaaacctg ccacacgcca gacaagtcac cccatgaacc
     5401 ccgggccttt gatccgcttg caatcccttt gcctgctgag agggaagaat ttcatcgtca
     5461 gaagtctaaa actctgcacg attattcatc gatgaacgat gcttgctccg ggaatgacgg
     5521 tcccaccctg cggcagggaa tcatccaggc agtagtactt gcggcaatcg caatggcagt
     5581 tatcgacggg atcgtggtga gcatcgccct cccatcgatc acgagggact tctcttccga
     5641 cgtggccctt tctcagtgga tcatcaccgc ctacctcgtt accgagacga gtctgctcct
     5701 catcttcggg aggctctcgg agttcactgg gaagaggaca ctctttttgg ccgggctcgt
     5761 gctcttcacc acgagttctc tggcctgcgg ccttgcagcc agcctgctcg agctcatcgt
     5821 atttcgcgtg atccaggcca gcggttccgc catgatattc tccataagcg cggccatcct
     5881 ctttgagact tcggtccccg gcagccaggg gaaggtgatg ggctacatcg gggcggtcac
     5941 ggccgcggcg ggcctcgcgg ctccgatcct gggcggtttc atcaccgacg ccctgggctg
     6001 ggcatttatt ttcctgataa acgtccccat aggggctctc ggcctggttc tcttcgcccg
     6061 ccattctcca cccggtgaaa ggcgcgacac gtccatacgg atggactggg ccgggtctgc
     6121 tacccttgtc atcgcgctcc tctttcttgt actcttcctc gtccgggtgt catctgctct
     6181 ctcgtggtct gccgagtcaa tggtattcct ccttgtatca accctctcca tgatcctctt
     6241 cctggtcgca gaatccagga gctcccatcc actccttgac ctctccatat tcaggaagct
     6301 cggttttact cttcccaatc ttgccatggt atgcatctac atggcattct tcatgctgaa
     6361 cctcatcgga cctttctatc tcgagggggt gatggacctg acaccatccc aggtagggct
     6421 cgtcttcctg atcgcccctc tgatcatggt cattgcttcc cccctctcgg gctggctgta
     6481 tgatcgatat catctctttt cactccccac actgggaatc ctgctcaccg gggtttcact
     6541 gcttctcctc gggttcgtgg tggtgcgcca ggatctcctg ctgattgtcc tcctgttcat
     6601 cccactctcg gttggaatct cgctcttcca cagcccgagc agcaccgata tcatgcgggc
     6661 gcttcccatc gcaaagtcgg gacttgcatc cagcgtgagt gcaacccttc ggaacctggg
     6721 gatgacatta ggcgtctctg tggcaggaat actcttcaca aaagaacttt atgatacagg
     6781 atataccgga ccggtccagg aagcagccgc gggcgttctt gcaggagtga ttggaaacct
     6841 tctctttatt gcagcggcgt tgtgcggtgt aggagccgcg ctttacctgg caaagaacat
     6901 ccttatcgac cgtgtctgaa gaagcggaaa atttacaatc ctgcccccat gcgcaatccc
     6961 gaaactggga ggggatgcta ttatgaagga ttcccgcaat ctccctttca gggattccac
     7021 atgaagattc tatatgtcta tgctcacccg gaaccaagat cattcaacgg tgcactcaag
     7081 gatgctgcca tggtcacgct tgccgtaaaa gggcacgatg tgaaggtctc gaatctctat
     7141 gcggtcaggt tcaaggccat tctggacgca caggatttcc ggatcagggg ccggcacgac
     7201 atcttcaacc ctgcagcgga acagatccac gggatacgca ccgggagcct ggctcccgac
     7261 atacaggaag agattgagcg tgtgtcatgg
//
LOCUS       DAOQVJ010000010         7283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_11261_length_7283_cov_4.732014, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000010 DAOQVJ010000000
VERSION     DAOQVJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11261_length_7283_cov_4.732014"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PKL26_00455"
     CDS             complement(<1..937)
                     /locus_tag="PKL26_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNS82196.1"
                     /translation="MSELDAYRSLYIAESRENHENIVKNLLVLEGEASSEAIAEIFRS
                     AHSLKGMSASMDFKGMERLCHAMEDVFQEIRNGNMAVTHALMDTLLSASDTMEAMLDD
                     IEAGGPGIPDDLDDLVEGLKKWIGLKCDALPKNDTLKSSDTAEGTQSGELISAPGGNA
                     QGLNNYCITLCLSDQCDNRNLRAMIILQNLEELGRLERVVPDRAIVEDGEFDGRFTVE
                     IASDAGIEAIKTVTSSSDVKEAVVEDLKSPAPSPAVIMKSAESPGEREEERRSPQRTE
                     KVEKGREVKNIRVDISRLDVMMNLVEDLVINRGRIT"
     gene            complement(941..1969)
                     /gene="cheB"
                     /locus_tag="PKL26_00460"
     CDS             complement(941..1969)
                     /gene="cheB"
                     /locus_tag="PKL26_00460"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNS82197.1"
                     /translation="MIRVLVVDDSLFMRTMIRDMLEKDPDIEVVATAVDGVDALKKIS
                     ELKPDVVTLDIEMPRMNGLEVLKSRSQFRDFPRVMMLSSLTSEGAEMTRKAMLLGADD
                     FMPKPKDMHAIKGIERELLEKIHNLVNIAYIVRPERRKAHLADRVVVVGASAGGPPML
                     DILISSFKASIPAAMVITQHMPEGGFTASLATRLNRISPMPVKETENGDVLNQASIYI
                     SRAGHHSVISALLTDTGEKGGRIVHSHSPPLHSVRPAVDKTFVSASSVFGERVVSVIL
                     SGMGNDGGEGMAVVREHGGRTIVCREEDCLVYGMARSALSKNCVEQVLPLEKIGEEIT
                     RLVAGMAN"
     gene            complement(1974..2336)
                     /locus_tag="PKL26_00465"
     CDS             complement(1974..2336)
                     /locus_tag="PKL26_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS82198.1"
                     /translation="MGRILIVDDTLFMRTLLKNILFSGGHDIVGEAGDGDEAIAKYQE
                     LKPDLVTMDVVMPKVNGIEALKGIKTIDPNAKVVMCTAVGQEQMVKLAIKTGAKGYIV
                     KPFQAPKVLEEIKNVLGS"
     gene            complement(2480..3286)
                     /locus_tag="PKL26_00470"
     CDS             complement(2480..3286)
                     /locus_tag="PKL26_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheF family chemotaxis protein"
                     /protein_id="HNS82199.1"
                     /translation="MAVVPVKIEKDGKWVVSKIDLQQDRMSIAEPLQKAIPFKSVVDL
                     EQKKNLLIITVKGEPPEVFRIASVDKVLQAMKRLIIMSCSAYRLTAYFMSPAVRGGVM
                     VTNATWEKGAIAVLKTSIWFVSQNKQVSVPLNEVTGIELTKREVQGKQVDVVKIDHLE
                     NSDVVTSYVLCPLSTLQILLNFLQDATKDLETSGEELDPLSAQVGMLIYSGMDSHAIE
                     NMLNISHKELDNIYEKLLKIGLIEVVFVRKEVQLTPKGVRFITDAVKPPT"
     gene            complement(3312..4469)
                     /locus_tag="PKL26_00475"
     CDS             complement(3312..4469)
                     /locus_tag="PKL26_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82200.1"
                     /translation="MKDSTLKLILFAVIIVFGSLMILFDIPVFYLLIGAVVLGILVLI
                     LAGGVKMPSIKRDRAKKEVGTPQDQAKKKAKEKKPPKEKKAKAPGTKSGVGDFFSSLK
                     GAFSVLGRDLGRKKKSKAELETHEKKIDSLLDQSIQGAAVASLDDIIPDAAPAEKKKT
                     SDPFTALVGEDLNADLLNGIQGAEDFSILDDSELGIEAGVEDQSQLSAQGDMSSLDIT
                     LSGDDDTITLDETNDADEVKEILEAHSGELGLDQDAGMSLAEDAMEGLDGLELEGMDL
                     GEEGEGPAPSAAAAPAGKTAATTLASPPGRSEPPKPAAPAFSPEQDMLSFSKGRGQDD
                     DLMASLRSDAKSTKKSEYASLIRDMKDIRVDASDLQSELEGLLKPRRPKPE"
     gene            complement(4471..6069)
                     /gene="flaJ"
                     /locus_tag="PKL26_00480"
     CDS             complement(4471..6069)
                     /gene="flaJ"
                     /locus_tag="PKL26_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellar assembly protein FlaJ"
                     /protein_id="HNS82201.1"
                     /translation="MAFAGFGKSVRSANAGKLPFQDLGTSVKSHLATLTENKQMGPDL
                     LFMVTYMASITTAQATRPEIFAYTAGRTEYVTCKYIQRVEFFVKRWNYSYVEGLSIIA
                     EKAKNDMLRSMLHRYGNSIESGVPDEDFLTRELATIRSVFRNAYEQGLEMLKKWSDAY
                     IAMLFSANLIGIIIMLSIAIFAPDDVQQTLRSAYFIVITIAIFGLVTMYKSVPSDDKT
                     HALQRCSKEQAIVRKLEMKVIPVALIAAILLYFISQNAGLSMLFLGFILMPLGVIGYL
                     DDRNIVLRDAEFTTFIRSLGAVMGGKGITVGSALAEIDRKSMENLEPFIAGVYSKINL
                     GLDEKLSWDRFVGESGSNLIYKYLNIFRDAVELGGKPDIIGQIVGSSMLEQVLLREKR
                     NSIAMGFVILLVPMHAMMVGIFLFLYHIMVMMSKAISSVVAELGSSSSALSNPGTVGG
                     SLGGSGLSMFVNFPEQEVGLFVVIMIIIITIANVIAGKIVMGGDRYMYYFFSSLLLLV
                     TGIIYIAAPVIVAMFFNIPTFQGV"
     gene            complement(6070..>7283)
                     /locus_tag="PKL26_00485"
     CDS             complement(6070..>7283)
                     /locus_tag="PKL26_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNS82202.1"
                     /translation="YRALEYMIIRDKIDVGLLKPFINDRYIEDITVDGVGPIFVEHKI
                     FKGLKSVVGWDNTDELDNFVIKLAERTKRPITYRNPIVDATLPDGSRINIVYSTDISR
                     KGSNFTIRKAMDDPISILKLIEFKTCDYLVAGYLWICIENGMSLFMSGETASGKTTSL
                     NAFTAFIPPENKIVTIEDTPELQIPHKNWTREVSKGKGKGEGEGSDVTMFDLLKAALR
                     QRPNQILVGEIRGVEGAVAFGAMQTGHPVLSTFHAASVEKLIQRLCGDPINIPKTYVD
                     NLNIVVIQSAVKRPSGETVRRILSVNELVGYNPETQGFSFVEMFHWNPATDLHEFPGR
                     GSSYLLENRIATMLGIPEHKKASIYDEVEKRARILERLHKAGYTGFYDLYFMMTKVKK
                     QGLLKIDVDAM"
BASE COUNT         1450 a         2090 c         1943 g         1800 t
ORIGIN      
        1 cggtgatgcg tccgcggttg atcacaaggt cctcgacgag gttcatcatc acgtcgaggc
       61 ggctgatgtc gactcggatg ttcttgactt cccgcccctt ttcgaccttt tcggtcctct
      121 gcggggatct tctctcttcc tccctctctc ctggggattc ggcggacttc atgataactg
      181 caggagaggg agcagggctt ttcaggtctt caacgaccgc ctctttcaca tccgaagagg
      241 aggtgacagt ctttatggcc tcgatccctg cgtcgcttgc gatctcgacc gtgaaacggc
      301 cgtcaaattc cccgtcttcc acgatggccc ggtcggggac caccctctcc agtctcccga
      361 gttcctcgag gttctggagg atgatcatgg cacgaaggtt ccggttgtcg cactggtcgc
      421 tcaaacagag tgtgatgcag taattattca gtccctgggc attgccccca ggggcagaaa
      481 tcaattcccc gctctgtgtg ccttctgccg tatcggagga tttgagtgta tcatttttgg
      541 gaagcgcatc acatttgagt ccgatccact tctttaaacc ctcgacaagg tcatcaaggt
      601 catccgggat gcccgggccg cctgcctcga tatcatcgag catcgcctcc attgtatcgg
      661 atgcggagag gagggtgtcc atcagggcgt gggtgactgc catgttcccg ttccgtatct
      721 cctggaagac atcttccatt gcatggcaaa gccgctccat ccccttgaag tccatggacg
      781 ctgacatgcc cttgagagag tgggcagaac ggaatatctc ggcgattgcc tcacttgagg
      841 cctcgccttc cagcacaagg aggttcttca cgatattctc gtggttctcc cgtgattcag
      901 cgatgtataa ggacctgtat gcatcaagtt cagacatgct tcagttcgcc atccccgcta
      961 ccaggcgtgt gatctcttcc ccgatttttt ccaggggcag cacctgctcc acgcagttct
     1021 tcgagagcgc ggaacgtgcc atcccataca caagacagtc ctcctcgcgg cacacgatag
     1081 tccggccgcc atgctcacgc accactgcca tcccttcacc cccatcgttc cccattcctg
     1141 aaaggatcac cgagacgact ctctcgccaa acacgctgga tgccgagacg aacgtcttgt
     1201 caacggcagg acgaacgctg tgaaggggag gggaatgcga gtggacgatc cttccccctt
     1261 tctcacccgt atctgtcagg agcgccgata tgacggaatg gtgccctgcc ctcgagatgt
     1321 agatactcgc ctggttgagc acatctccat tctcagtttc cttgacaggc atcggagaga
     1381 tgcggttcag gcgggttgca agggaggcgg tgaaccctcc ctcgggcatg tgctgggtga
     1441 tcaccattgc tgcagggata ctggccttga acgaagatat gaggatatcg agcatgggcg
     1501 ggcctcctgc cgacgcaccc accaccacga ccctgtctgc gagatgcgcc tttctcctct
     1561 cggggcggac gatatacgcg atattgacca gattgtgtat cttttcgagg agttcgcgct
     1621 cgatgccctt gatcgcatgc atatcctttg gtttcggcat gaaatcgtcc gcaccgagca
     1681 gcatggcttt cctggtcatc tctgcgcctt cggatgtgag ggatgagagc atcatcaccc
     1741 gcggaaaatc cctaaattgc gacctgctct tcaatacttc gagcccgttc atcctgggca
     1801 tctcgatgtc aagggtgacg acgtcaggct tcagctcgga gatcttcttg agtgcgtcca
     1861 ccccgtctac cgctgttgcc actacctcga tatcgggatc tttctcaagc atgtccctga
     1921 tcatggtgcg catgaagagg gagtcgtcca ccacgagaac cctgatcatc ttctcatgaa
     1981 ccaagcacgt tcttgatctc ctccagcact tttggggcct ggaatggttt cacgatgtag
     2041 cccttcgcgc cagtcttgat cgcaagtttt accatctgct cctggcctac cgccgtgcac
     2101 atgaccactt tcgcgttcgg gtcgatggtc ttgatccctt tcagggcctc gatcccgttc
     2161 accttcggca ttacgacgtc cattgtcacc aggtccggct tgagctcctg gtacttggca
     2221 atggcctcgt cgccgtcacc cgcctctcct acgatgtcat ggccgccgga aaagaggata
     2281 ttcttgagaa gggtacgcat gaagagggtg tcgtccacta tgagaattct ccccatatct
     2341 atcactatac aggtaaatgc aaagaattta aatagttaat cgtggaggcc ctatcagact
     2401 ggtggcatat ccaaaaaaat ccgcccgctt cttggcattc ctggttcact tcaccccgaa
     2461 atgcaaatat cggcagttcc taggttgggg gttttacggc atctgtgatg aaacggaccc
     2521 ccttcggggt cagctggacc tcttttctta caaatactac ctctattaaa ccgatcttta
     2581 aaagtttctc gtaaatattg tcgagttcct tgtgggagat gttcagcatg ttctctattg
     2641 cgtgcgagtc catcccgctg tagatgagca tgcccacctg ggcagagagg gggtcgagtt
     2701 cctccccgct tgtctcgagg tcctttgtgg catcctgaag gaaattgagc aggatctgca
     2761 gggtggagag tgggcagagc acgtaacttg tcactacatc ggaattttcg aggtggtcga
     2821 tctttaccac atccacctgc ttgccctgca cctcccgctt ggtcagctcg atcccggtaa
     2881 cctcgttgag cggaaccgac acctgcttgt tctggctcac gaaccagatg ctggtcttga
     2941 ggactgcgat ggctcccttc tcccaggtgg cgttcgtcac catcacgcca cccctgaccg
     3001 caggagacat gaaatatgca gtcaggcggt aggcgctgca ggacatgatg atgagccgct
     3061 tcattgcctg gaggacctta tcgacactcg cgatccggaa aacctccggg ggctctccct
     3121 tgacggtgat gatcaacagg ttctttttct gctccaggtc cacgacagac ttgaatggga
     3181 tggccttctg gaggggctca gcgatggaca tcctgtcctg ctggaggtcg atcttgctga
     3241 ccacccattt gccatccttc tcgatcttta cggggaccac tgccatgctt cctttcacct
     3301 gtctgaaaaa gttattctgg ttttgggcgc cttggtttca ggaggccctc gagctcgctc
     3361 tggagatcag atgcatcaac cctgatatct ttcatatccc tgataaggct tgcatattca
     3421 ctcttcttcg tggattttgc atccgaacgg agcgacgcca tgaggtcgtc gtcctggccc
     3481 ctccccttgg agaacgagag catgtcctgt tccggtgaga atgcgggggc agcgggtttt
     3541 ggaggttcgg atctccccgg cggcgatgcg agcgttgttg ctgccgtttt tcccgcggga
     3601 gcggctgctg ccgagggtgc agggccctct ccctcttctc caaggtccat cccctccagt
     3661 tcaagaccgt caagtccctc cattgcatcc tcggcaaggc tcatcccggc atcctggtca
     3721 agtccaagtt cgccgctatg tgcctcgagg atctccttca cctcgtctgc atcgtttgtc
     3781 tcgtccaggg tgatggtgtc gtcatctccg gaaagggtga tatcgaggga agacatgtct
     3841 ccctgcgcgg aaagctgcga ctggtcctca acccctgcct cgattccaag ctcgctgtcg
     3901 tcgaggatgg agaagtcttc cgctccctgg ataccattga gaaggtccgc gttgaggtct
     3961 tctcccacga gtgcagtgaa cgggtcggag gtcttcttct tctctgccgg cgcggcatcg
     4021 ggaatgatat catcgagcga tgccactgcc gctccctgga tggactggtc aaggagggag
     4081 tcgatctttt tctcgtgcgt ctccagctcc gcttttgact tctttttcct acccaggtct
     4141 cgcccgagga ccgagaacgc acccttgagg gatgagaaaa aatcgccgac accgctcttc
     4201 gtgccgggtg cttttgcctt cttctctttt gggggcttct tctccttcgc cttctttttc
     4261 gcctgatcct gcggggttcc cacctctttc ttcgcgcgat ctcttttgat tgaaggcatc
     4321 ttcacccccc cggcaaggat cagcacaagt attccaagga ccaccgcacc gatcaggagg
     4381 tagaagacag ggatgtcgaa gaggatcatg agtgaaccaa atacgatgat taccgcgaaa
     4441 agaatgagct tgagggttga atccttcatt tcagactcct tggaaggtgg gaatattgaa
     4501 gaacatagca actattaccg gcgcggctat ataaatgatg cccgtgacga ggaggagaag
     4561 gctgctgaag aaataataca tgtaacggtc tccccccatc acgatcttcc ccgcaatcac
     4621 attcgcgatg gtgatgatga tgatcatgat cactacaaag aggcccactt cctgttcagg
     4681 gaagttcacg aacatggaga gtccgctccc cccaagcgat ccgcccacgg tcccggggtt
     4741 gctcagtgcc gaggaggagg acccaagctc cgcgaccacg gaggagatgg cctttgacat
     4801 catcaccatg atatgataca ggaagaggaa aattcccacc atcatcgcgt gcatggggac
     4861 aagaagtatg acaaacccca ttgcaatgga gttccgcttc tccctgagga ggacctgttc
     4921 cagcatggag gatccgacga tctgcccgat gatatcgggc tttcctccga gttccaccgc
     4981 gtccctgaag atattgaggt acttgtagat gaggttgctt ccggactccc ccacgaacct
     5041 gtcccagctg agtttctcgt caaggccaag gttgatcttt gagtataccc ccgcgatgaa
     5101 aggctcgagg ttctccatcg atttccggtc gatctcggcg agggcgctcc ctacggtgat
     5161 cccctttcct cccatgaccg ctcccaggct cctgatgaac gtggtgaact cggcatcgcg
     5221 caggacaatg ttcctgtcat cgaggtaccc tatcacgcca aggggcatga ggatgaatcc
     5281 gaggaagagc atcgagagcc ccgcgttctg ggagatgaaa tagaggagta ttgcagcgat
     5341 caacgcgaca gggatcactt tcatctccaa tttccgcacg atggcctgct cctttgagca
     5401 ccgctgcagg gcatgggtct tgtcgtcgga tgggacggac ttgtacatgg tgacgaggcc
     5461 gaagatcgcg atagtgatca cgatgaaata ggcggacctg agcgtctgct ggacatcatc
     5521 gggcgcaaag atcgcaatcg agagcataat gatgatgccg atgaggttgg cggagaagag
     5581 catggcaatg taggcatcgc tccatttctt cagcatctcc aggccctgct cataggcatt
     5641 cctaaagacg ctccggatcg tcgcaagttc ccgggtgagg aagtcctcgt cggggacgcc
     5701 ggactctatg gaatttccgt agcggtggag catgctccgc agcatgtcgt tcttcgcttt
     5761 ctcggcgatg atcgagagac cctcgacgta actgtaattc cagcgcttca caaagaattc
     5821 aacgcgctgg atatacttgc acgtgacata ctccgtacgt cccgcggtat atgcgaaaat
     5881 ttcaggcctg gtcgcctggg cggtcgtgat ggaggccatg taggtgacca tgaaaaggag
     5941 gtccggcccc atctgcttgt tctcggtgag ggtggccagg tgactcttta ctgatgtgcc
     6001 aaggtcctga aacggaagct ttcccgcatt cgccgatctg actgactttc cgaaccctgc
     6061 aaacgccatc tacatcgcat cgacgtcgat cttcaggagt ccctgcttct tcacctttgt
     6121 catcatgaaa tacaggtcgt agaatcccgt atatcctgcc ttgtgcagcc gctcgaggat
     6181 cctcgccctc ttctcgacct cgtcgtagat tgacgctttc ttatgttcgg gtatgccgag
     6241 catcgtggcg atcctgtttt caaggagata gctgctccct cggcccggaa attcgtgcaa
     6301 atcggtcgca gggttccagt gaaacatctc tacgaacgag aacccctgtg tctcggggtt
     6361 atagccgaca agttcattca cgctcaggat ccgccggact gtctctccgc tgggacgctt
     6421 cactgcgctc tggatgacca cgatgttgag gttgtcgacg taggtctttg gtatgttgat
     6481 cgggtccccg cagagacgct ggatgagttt ctcgacggat gcggcatgga acgtggagag
     6541 gaccgggtga cctgtctgca ttgccccgaa tgcgaccgcc ccttcgacac cacggatctc
     6601 gcccaccagg atctggttcg gccgctggcg gagcgcggcc ttgaggaggt caaacatggt
     6661 gacatcagag ccttctcctt cacccttccc cttccccttc gagacctcgc gcgtccagtt
     6721 cttgtgcggg atctggagct caggggtatc ctcaatcgtc acgatcttat tttcgggcgg
     6781 gatgaatgcg gtgaaggcat tgaggctggt ggtctttccg cttgccgtct ctccgctcat
     6841 gaagagcgac atcccgttct cgatgcatat ccagagatag cccgcgacca ggtagtcaca
     6901 ggtcttgaac tcgatcagct tcaggatgct gatggggtcg tccatggcct tacggatggt
     6961 gaagttactc cccttcctgc tgatgtcggt gctatacacg atattgatac gtgacccatc
     7021 aggaagagtg gcatctacga ttgggttccg gtaggtgatc ggtctcttgg tccgctctgc
     7081 gagcttgatc acgaagttat cgagttcatc ggtgttgtcc cagcccacca ccgacttgag
     7141 ccccttgaag atcttgtgct cgacaaagat gggcccaacc ccgtccaccg tgatatcctc
     7201 gatgtaccgg tcattgatga acggtttcag tagcccgaca tctatcttgt cgcggatgat
     7261 catgtattcg agcgccctgt att
//
LOCUS       DAOQVJ010000011         6922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_12054_length_6922_cov_3.779234, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000011 DAOQVJ010000000
VERSION     DAOQVJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12054_length_6922_cov_3.779234"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PKL26_00490"
     CDS             <1..1413
                     /locus_tag="PKL26_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNS82203.1"
                     /translation="GLDHAKKWEFKPLAKEGATVAPGSIIGEVQETNIVHRVMIPPNV
                     KGGTIKTIKAGMFTVDEVVCTLDNGAQISMMQKWPVRVPRPVNEKMNPTVPLITGQRI
                     LDGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTEF
                     PELEDPKSGKPLMERTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYDVSLMADST
                     SRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFYERAGRVKTLAGSMGSVSVIGAVS
                     PPGGDFSEPVTQNTLRIVKVFWALDAKLSQRRHFPAINWLNSYSLYLDSLHDWYDKEV
                     SPEWNTIRSWAMEILQKEAELQEIVQLVGSDALPEAEQITIEVARMIREIFLQQNAYD
                     AVDTFCDMKKQYDMMKAIRQYSDLAYAAQSAGVTPQQILAVKAKNELPQIKFIKDYKP
                     ALDKIQAELEAEFNALRSAA"
     gene            1410..2801
                     /locus_tag="PKL26_00495"
     CDS             1410..2801
                     /locus_tag="PKL26_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNS82204.1"
                     /translation="MKEYRTITQIAGPLVFVEKTEPVGYGELVNIVLFDGTIKRGQVL
                     DTSDELVVVQVFETTAGIGKDSGIRFTGETIKMPVGRDMLGRILSGGGKPIDGGPEIV
                     PEKRLDITGAAINPYARGSPNDFIQTGISTIDGTNTLVRGQKLPIFSGAGLPHNQIAL
                     QIARQSKVPGSKEAFAVVFAAMGITKEEANYFMQDFERTGALERAVVFLNLADDPAVE
                     RIITPRLALTTAEYLAFDLGMHVLVILTDMTNYCEALRQIGAAREEVPGRRGYPGYMY
                     TDLAGIYERAGIVKGKKGSITQFPILTMPGDDITHPIPDLTGYITEGQIVVSRELHRK
                     GIYPPINVLPSLSRLMNLGIGKGHTREDHKKVSDQMYAAYAEGNDLRGLVAIVGKDAL
                     SERDRMFLEFADIFEGRFVRQGYSEDRTIEDTLTIGWNLLSSLPVEQLVRIDRDLINK
                     YHPKFAQAGKAEG"
     gene            2808..3434
                     /locus_tag="PKL26_00500"
     CDS             2808..3434
                     /locus_tag="PKL26_00500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNS82205.1"
                     /translation="MALRDIKPTRSELISLKKRIALSERGYKILKMKRDGLILEFFKV
                     LEQAKNTRGDLNERYESAQAMMAVANTVEGAIGVKAAAFSVQQEPDIKVASKNIMGVV
                     VPEIESSKVRKGLNERGYGILGTTSVIDETALSFEELVESIIRSAEIETTMKKLLDEI
                     ESTKRRVNALEFKVIPELTEARDFIKMRLDEMEREELFRLKKIKARNV"
     gene            3450..4667
                     /locus_tag="PKL26_00505"
     CDS             3450..4667
                     /locus_tag="PKL26_00505"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNS82206.1"
                     /translation="MPIGTLKEIKPEGKTILLRLDLNSPIDPTTRHILDDKRFREHLP
                     TIQALSQSRVVIVTHQSRPGKKDFTTLEAHAERLEALLQRRVKYVDDIFGQCARDAVR
                     SMKNGDVLVLENVRFNAEENLTVKAEDAQKLYLVKKLAQMGDFFVNDAFGTAHRSQPT
                     MVGLPMVMRSVAGLLMEKEVSTLTRVFQNAPRPVCLVLGGTKVDDSISVAEHMLKGNT
                     ADLIIVTGVVANIFLSASGFDIGRPSTQLIQQLKYEGEIARARALLSSFKDRILLPGH
                     VAVKENGKRAEYPVNAIPGEAPVMDAGVDSIAQLSGAIRKCGTVVFNGPAGVFEEPDF
                     ATGTYELLRTASKVEFSIIGGGHTAAVVEKMGIDRNFTHISTGGGACIEFLTGKKLPA
                     VQALERSRELFGG"
     gene            complement(6603..>6922)
                     /locus_tag="PKL26_00510"
     CDS             complement(6603..>6922)
                     /locus_tag="PKL26_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82207.1"
                     /translation="RYAVIFYWPLYIVGAAMLVPGTRIMTVAGIATLFGLVLFMITAL
                     IFSVATYNLMRSKSRGSSSPSIMVSFGKPGSFMRAFRLPAHGRKSHSTRSSQASRAAV
                     VED"
BASE COUNT         1680 a         1922 c         1911 g         1409 t
ORIGIN      
        1 ggtcttgatc atgcgaagaa gtgggagttc aaaccgctgg caaaagaagg cgcaaccgtt
       61 gcccctggga gcattatcgg cgaagtccag gagacgaaca tcgtccaccg ggtcatgatc
      121 ccgcccaatg tgaagggcgg aaccataaag accatcaagg ccggcatgtt cactgtcgac
      181 gaggtggtct gcaccctgga taacggcgcc cagatctcca tgatgcagaa atggcccgtc
      241 cgtgtacccc ggcctgtcaa cgagaagatg aaccctacgg ttccccttat caccgggcag
      301 cgtatcctcg acggtctctt tcccatcgca aagggcggga ccgcggcgat ccccgggccg
      361 ttcgggagcg ggaagacggt cacccagcag cagctcgcaa agtggagcga tgccgagatc
      421 gtggtctaca tcgggtgtgg tgagcgcggc aacgagatga ccgaggtgct gaccgagttc
      481 ccggagctcg aggacccgaa gtcaggaaag cccctgatgg agcggactgt gctcatcgca
      541 aacaccagca atatgccggt ggcggcccgt gaggcatcgg tgtataccgg gatcaccatc
      601 gccgagtact tcagggatat ggggtacgat gtctcgctga tggcggactc cacctcccgg
      661 tgggcggaag ccatgcgtga gatctcctcg cgtcttgaag agatgccggg agaagagggg
      721 taccccgcgt atcttgcagc acgcctctcg gagttctacg agcgggccgg gagagtaaag
      781 acccttgccg gaagcatggg gtcggtgtct gtcatcggag ccgtatcccc ccccggagga
      841 gacttctccg agcctgtcac ccagaacact ctccgtatcg tgaaggtatt ctgggccctc
      901 gatgcaaagc tctcccagcg caggcacttc cctgccatca actggctcaa ctcctactcg
      961 ctgtaccttg acagcctcca tgactggtat gacaaggaag tgtcccctga atggaacacc
     1021 atccgctcat gggccatgga gatcctccag aaggaggctg aactgcagga gatcgtgcag
     1081 cttgtcgggt cggacgccct tccggaggca gagcagatca ctatcgaggt ggccaggatg
     1141 atccgcgaga tcttcctgca gcagaacgcc tacgatgcgg tggacacctt ctgcgacatg
     1201 aaaaagcagt acgacatgat gaaggcgatc cgccagtact ccgaccttgc gtacgcggca
     1261 cagagcgccg gcgtgactcc gcagcagatc ctggccgtca aggccaagaa cgagctgccc
     1321 cagatcaagt tcatcaagga ctacaagccc gcacttgaca agatccaggc tgaactggaa
     1381 gcagaattca acgcactgag gtcggcggca tgaaagaata cagaaccatc acccagatcg
     1441 ctggtcccct cgtcttcgtc gagaagaccg aaccggtagg atacggcgaa ctggtcaaca
     1501 tcgtgctctt tgacggcacc atcaagaggg gacaggtact tgacaccagc gacgagctgg
     1561 tggtggtaca ggtcttcgag accacggcgg ggataggaaa ggacagcggg atccgcttca
     1621 ctggtgagac gatcaagatg ccggtcgggc gtgacatgct gggccgcatc ctctccgggg
     1681 ggggaaaacc aatcgatggc ggacccgaga tcgtaccgga gaaacgcctg gacatcaccg
     1741 gcgccgcaat caatccctac gccaggggct ccccgaacga ctttatccag accggtatct
     1801 ccacgatcga cggtacaaat acccttgtca gggggcagaa gcttccgatc ttctctggtg
     1861 caggacttcc tcacaaccag atcgctctcc agattgccag gcagtcaaag gttcccggct
     1921 cgaaagaggc cttcgccgtg gtctttgccg caatgggaat caccaaggag gaggccaact
     1981 acttcatgca ggacttcgag aggacgggag cactcgagcg tgccgtcgtc ttcctcaatc
     2041 ttgcagacga ccctgcagtc gagcgtatca tcactccgcg gcttgcactg acgaccgccg
     2101 agtacctcgc gtttgatctt ggcatgcacg tcctggtcat cctgaccgac atgaccaact
     2161 actgcgaggc gctcaggcag atcggtgcgg cccgcgaaga ggtgccgggt cgccgtggct
     2221 atcccggata catgtatacc gaccttgccg gcatctacga gcgtgcgggg attgtgaaag
     2281 gtaaaaaggg ctccatcacc cagttcccga tcctcaccat gccaggtgac gatatcacgc
     2341 acccaatccc cgaccttaca gggtacataa ccgagggcca gatcgtggtg agcagggagc
     2401 tgcaccggaa gggcatctac ccgcccatca acgtgctgcc atccctctcc cggctgatga
     2461 acctcgggat tggaaaaggg cacacccgcg aggatcacaa gaaagtatcc gaccagatgt
     2521 acgccgccta cgccgagggc aacgacctcc ggggcctggt ggcgatcgtc gggaaggatg
     2581 cactctccga gcgggaccgg atgttcctcg agtttgcaga catcttcgag ggccggttcg
     2641 tgcgtcaggg atacagtgag gaccggacta tcgaggatac cctcaccatt ggctggaacc
     2701 tcctgtcgtc actgcctgtc gagcagctgg tgcgtatcga ccgcgacctg atcaacaagt
     2761 accaccccaa gttcgcgcag gccggaaagg ctgaggggta agggaccatg gcactccgag
     2821 acatcaagcc caccaggtcc gagctgatca gccttaaaaa gaggattgcc ctctcagaga
     2881 gagggtacaa aatcctcaag atgaagcggg acgggcttat cctggagttc ttcaaagtgc
     2941 tcgaacaggc aaagaacacg cggggcgacc tcaatgaacg gtatgagagc gctcaggcca
     3001 tgatggcagt cgcaaatacc gtggaaggag caatcggtgt caaggcggcg gctttctctg
     3061 tccagcagga gcctgacatc aaagtggcca gcaagaacat catgggcgtt gtggtccccg
     3121 agatagagtc ctcgaaggtg cggaagggct tgaacgagcg gggctatggg attctcggga
     3181 ccacctctgt cattgacgag acggcgctct cgttcgagga gctggtggag tcgatcatcc
     3241 ggagcgcgga gatcgagacc acgatgaaga agctccttga cgagattgag tctacgaaac
     3301 ggagggtcaa tgccctcgag ttcaaagtga tccccgaact gaccgaggcc agggacttca
     3361 tcaagatgcg ccttgacgag atggagcggg aagaactctt ccgtctgaag aagatcaagg
     3421 caaggaacgt gtagggggag ggaaagaagg tgcccatcgg gaccttgaaa gagataaaac
     3481 cggagggaaa gaccatactt ctccggttgg acctcaactc ccccatcgac cccaccacca
     3541 ggcacatcct tgatgacaag cgtttccgcg agcacctccc tacaatccag gccctctccc
     3601 agagcagggt agtgattgtc actcaccaga gccggccggg aaagaaggac ttcaccaccc
     3661 tggaagctca tgccgagagg ctggaggcgc tcctccagag gagagtaaag tacgttgacg
     3721 acatctttgg acagtgcgcc cgcgatgcgg tccggagcat gaagaatggc gatgtcctcg
     3781 tcctcgaaaa cgtcaggttc aacgccgagg agaacctgac agtcaaagcc gaagatgcac
     3841 agaagttata cctcgtgaag aagctcgccc agatggggga cttttttgta aacgacgcct
     3901 tcggcaccgc tcaccgttca cagcccacga tggtggggct cccgatggtc atgcggtcgg
     3961 ttgcaggact cctgatggaa aaggaagttt ccactctcac gagggttttt cagaacgctc
     4021 cccgtcccgt gtgccttgtg cttggcggga caaaggtgga tgactcgatc tctgtcgcgg
     4081 agcacatgct gaaaggaaat accgcagact tgatcatcgt gaccggagtg gtagccaaca
     4141 tattcctctc ggcttccggc ttcgatatcg gcaggccctc cacccagctg atccagcagc
     4201 tcaagtacga gggcgagatt gcgagggccc gcgcactcct ctcttctttc aaggacagga
     4261 ttctcctccc cgggcacgtg gcggtcaagg agaatggaaa gagggcagag tatcctgtca
     4321 acgccatacc tggagaagcc ccggtcatgg atgcaggagt ggactcgatc gcgcagctct
     4381 ccggagccat ccggaaatgc gggactgtcg tcttcaacgg ccctgcaggt gtcttcgagg
     4441 agcccgactt tgcgaccggg acctacgagc tcctgcggac cgcatcaaag gtcgagttct
     4501 ccattatcgg cggtggccat actgctgcag tggtcgagaa gatgggaata gaccggaact
     4561 tcacccatat ctccaccggc ggcggggcat gcatcgagtt tttgaccgga aagaagctcc
     4621 ctgcagtgca ggcgctggag aggtcccgcg agttgttcgg gggatgaaaa ctctgttctc
     4681 tttttcgaag tatattcatt tcctctaaaa tacaactgcc tgcgatacgc ttctaatctt
     4741 tacaaatatg tcttgtgagg atatggccat tccaaatggt tttatagggt tgaataaata
     4801 ttacgggtta taatgaatcc atatacactc aagggaatcg gatctatctt cctcatttgc
     4861 gcctttgtcg gattcgggca tataagtggt tatggggcag gtttttcttc aacaaaaata
     4921 tcgccacaat taaatgagaa attgacatca ttacaaaacc cggcatcaac ggatacaaat
     4981 atcatcaatt ctctatctgg cataaaatca ttgatggaac agcctggaac acttggcgcc
     5041 ttgatatccg tgcgccaaac tcgcggcctt atctatgcat gcacccctat tgacggaggg
     5101 ggcatattct gtaaatgggt gtatcctgat cccgaaatca ggggtgacca gttgcggggt
     5161 ttgtgaggta atatacatgc cgccctcccc tccgatgttc ccgaggctcc gccctgcacc
     5221 cttgcgcaat ccccaaatgc gatagggcta gtgctgggaa taaaccagaa tcagaggttt
     5281 tccagtttgc tcttcttgca gactatcgtg tatctgggga ggggtttgag gagggggatt
     5341 gtcccctccc ccctacgcga aaaccgcact ccatacacga tgacgctcca ggggctccgc
     5401 cccgcacgct tgcgcgaccc cccaatgcga tagggccagt gctaaacacc aggtaaaatc
     5461 taatttgtcc cagtgcaatc ctgcccgcag tatatctcat tcacaaaacg caaaccctct
     5521 ctgaacgcgc tgtatatacc atttctttta acgccggatt aaaatatttg agacacgtgc
     5581 tgtttatcgc gccatatatc tgacaacttc aggggaataa tcctgtgaaa aggtgtatat
     5641 ataaatatat tatatacaaa cctacatgaa tctctctaaa cctggaatta tcggatcaat
     5701 atttttcctc atatcatttt tgactttttc agcagtccct gtctatgcgg aaatgatttc
     5761 atcaaggtca tcgacacccc gactgagtga aataagcaag atgtcggcaa ttgagaagat
     5821 ggcggcaaat ccgttctcga atgagatata cattcccgat tttgggtatc acgatcaata
     5881 tatttccccc tggggggccg atgcatcaaa aaattatgaa agtaacgtca aagagtttgg
     5941 tgaagtgata gcgtcattct taatgcacaa gggtaactcg tattctgaat atcgcagtat
     6001 catgcgataa aatgattaat catccgctgc cgtaatgaag agtcccgatt atcgttgact
     6061 tatccgttgc atatccattc actagcccga tctcattcct aatccaggcg gcctgcactt
     6121 attccagatg ttccgatgca gctctgtcat caggttattg ccttgaatgg gatgggtcgg
     6181 tgaagaaggc ctctcgcccc gggacccgaa ggattgagat tttcctctta cccatgagag
     6241 agtccagtga atcctaatcg ataactaacc aaaccgcact ccacccacga ttgtacttct
     6301 cagggctccg ccctgcacgc ttgcgcgatc cccaaatgcg atagggccag tgctggaatc
     6361 ctttgtcgat cctaactcca tgcaaccctg cctgcaggct atcgtggatc agggggaggt
     6421 cctgaggggg attctccccc tccggtcaca agttccagtt taatctgttt ccacactgaa
     6481 cacaaccaca cactccatac acgatgacgc tctcgcggct tcgtcccgac actgtggcat
     6541 ccccaaaata agataaggac agtgctgggt gtgcagagaa aaagagagag aagggggagg
     6601 ccctaatcct caacaaccgc cgcccgggag gcctgcgagg accgggtaga atgagacttc
     6661 ctcccatggg caggcaggcg gaacgcgcgc atgaaacttc caggctttcc gaaggagacc
     6721 ataatcgaag gactgctgct cccccggctc ttcgatctca tcaggttgta ggttgcaacc
     6781 gaaaatatca gtgcggtgat cataaacagg acaagtccaa agagggtggc aatgcctgca
     6841 acagtcataa tgcgggtccc ggggaccagc atggcggcac ccacgatata cagcggccag
     6901 tagaatatca ctgcatacct cg
//
LOCUS       DAOQVJ010000012         6747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_12458_length_6747_cov_4.180663, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000012 DAOQVJ010000000
VERSION     DAOQVJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12458_length_6747_cov_4.180663"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..228)
                     /locus_tag="PKL26_00515"
     CDS             complement(40..228)
                     /locus_tag="PKL26_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HNS82208.1"
                     /translation="MGIKPTYIKAIGIELLNHHGNRFSNNFDENKQTITEVTNIESKR
                     VRNRVAGYITRKVNTKRR"
     gene            complement(246..800)
                     /locus_tag="PKL26_00520"
     CDS             complement(246..800)
                     /locus_tag="PKL26_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HNS82209.1"
                     /translation="MMSTPGDEREVVLRELEESVRALSLGMAVLLIPEVGSNIAYATP
                     TARDPSQVAAVEGRIVRLSGRAHPVGQVSFGASDHVARIVLTAMRFDPQVRCAANIRY
                     SPETVRILEEMLLEICSFDRSSEPPGAKTMDWGVASCCRDGVPDVIYDLGAVGKEPMI
                     RLLGENPDAVVKNILKVSARMNKE"
     gene            911..1087
                     /locus_tag="PKL26_00525"
     CDS             911..1087
                     /locus_tag="PKL26_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82210.1"
                     /translation="MDARVLDILSAVVSFIVLLVFLLVLPLFLEQGIAYLLAIVIFIL
                     TMSGAGFYINKTLS"
     gene            complement(1080..>1601)
                     /locus_tag="PKL26_00530"
     CDS             complement(1080..>1601)
                     /locus_tag="PKL26_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase regulatory subunit PriL"
                     /protein_id="HNS82211.1"
                     /translation="PLEAEELLKEQIRVTLLSQLPLDVPQSLCEEMAPYVSRIQDAFQ
                     QKMLEDFGEVEESCFPPCMQAIMSALSGGLNITHSGRFALTAFLHNIGMDGAQIIELY
                     CRAPDFDVSKTQYQVEHISGRGGTGTEYTAPSCAAMRTLGLCVRPDAGCEKVNHPLNY
                     YRSRKKGKKAKPN"
     assembly_gap    1602..1701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1702..2263)
                     /locus_tag="PKL26_00535"
     CDS             complement(<1702..2263)
                     /locus_tag="PKL26_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase regulatory subunit PriL"
                     /protein_id="HNS82212.1"
                     /translation="MQVSLETRDFAKYPFLKESQQFMLQNHESVDRFLQSSAGKVAIR
                     YAVERVQRALSHAGQTIEIERIPLPSDSLGVRLEVSSYALARLLVSCGKERALIDRLT
                     RYEALRACAFLEGEDDGKRAYVASQIGFSLGRLSLPVSRYVEITAAMREERWRLVNRE
                     VEDGNVAIEPLEAEELLKEQIRVTLLS"
     gene            complement(2263..3006)
                     /locus_tag="PKL26_00540"
     CDS             complement(2263..3006)
                     /locus_tag="PKL26_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HNS82213.1"
                     /translation="MLKATIDADILKESIDAISALVTECRLHTDENGLSTRAVDTANV
                     AMVSLTLKKEAFSAFESTKGEIGLDIAKMKNIFGMMGKNDVISLELPEDGHKLKVSFE
                     GYRYSITLLDPNTIRKDPNPPNIDLPGKVVLSGNDLNNSIKAAAVVSDKIALGIDPAQ
                     QLFFMAAEGDTDHIRREFGKNEVNFLNGMEARSLFSLDYLKDMGKVMSRAEEVEVSLG
                     IDHPAKFAFYIAGGHGYVEYLLAPRIEAD"
     gene            3107..3715
                     /locus_tag="PKL26_00545"
     CDS             3107..3715
                     /locus_tag="PKL26_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNS82214.1"
                     /translation="MGSQWGKDRTYLRAVREGYRSRAAYKLQEIQERYHLIREHDNVL
                     DLGASPGSWLQVARGLTAGRILGIDLSPIAPLEGVTTLIGDLTDRSLRDEARKVLGIV
                     NVIISDAAPKLSGHRSYDQARAIGLGQEVLSFACDVLKPGGNLVLKSFQGEDFQALLK
                     EMRQYFLSVKTFKCRASRRGSSEIYIIARNFVGCRNETEGSL"
     gene            3693..4571
                     /gene="moaA"
                     /locus_tag="PKL26_00550"
     CDS             3693..4571
                     /gene="moaA"
                     /locus_tag="PKL26_00550"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNS82215.1"
                     /translation="MKLKDPYNRPVSNLRISLTPACNLHCIYCHAEGEKDPKAPLSLE
                     ELAEIMKVAAKFDIGSVKFTGGEPCLRADLADIVRAVPERMECSMTTNGTLLADMAHD
                     LADAGLSRVNVSLDSLDPATYMKITGSDCLDDVLAGIDAATEARLTPIKLNMVLLKGM
                     NDHEVDEFIRFVRGNRDLILQVIELMEFRDCTNHHELKDLEVWLASNSQEILTRRMHH
                     RKKYCIDGAEVEVVRPLHNTEFCAYCNRLRVTSDGKLKPCLLRTDNHVDIRGKYGEEL
                     EELFREAVRRRAPFFQ"
     gene            4659..5162
                     /locus_tag="PKL26_00555"
     CDS             4659..5162
                     /locus_tag="PKL26_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82216.1"
                     /translation="MSSDAEYVHVLEHLYEKSLILQDETMWHPVLAFYYMDALAHLDY
                     VISLMAYHYKSPRVMMAGEYLRCRVDQEKLGDRPRFPAFVNWLKREHPKRFEALPTVW
                     RRVYDEDDEARYISFRIVFDRDSKDPIRPHVYRSLIEEFFNTEFLKTLYSDASLSALF
                     EEFKRKA"
     gene            5174..6349
                     /locus_tag="PKL26_00560"
     CDS             5174..6349
                     /locus_tag="PKL26_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HNS82217.1"
                     /translation="MNVTDLCADLVRIRSENPPGNTRDVIEYIRNFTDSLGIATQVIR
                     GPGGKDNLVSRTANGRLLFCGHVDVVPALDEGWTHDPFAGTIEDGKVWGRGTTDMKGG
                     CASVLWACRNWLDSGRELPADLAFVCDEETSGTHGIQALLATKCLVPCDTIVAEPTPS
                     LSPNVGQKGLMRLCCRFRGEPGHGSLYPAKGVSAVMEAFSLLEFVRLLHKREFSPADP
                     ALSGIIGESSKILGEIFGIEDTKRVLSHVMYNPGKIEGGEKANIVAQQCSLELDLRIP
                     WGCSLPDLLEEIRRHAPRGEISVTNSSDPTITPPDSPLVTRLLREIEKVHSATARPIV
                     QWAASDARYLRNAGFPVVEYGPGEIRTLHAIDEHVTIESLENAARVYEGMLGRYAMI"
BASE COUNT         1414 a         1907 c         1849 g         1477 t
ORIGIN      
        1 atagccggaa taactccctc gaacatacga gagaggtatt cagcgtcttt tggtatttac
       61 ctttctggtg atatacccgg caacccggtt cctcaccctc ttgctctcga tattcgtgac
      121 ttcagtgatg gtctgcttat tctcgtcaaa gttgttggag aaccggttcc catggtggtt
      181 cagcagctca attccgattg ccttgatgta ggtgggtttg attcccatgt ggtcattcct
      241 cttggttatt ctttattcat acgcgccgag actttaagaa tgttttttac gactgcatca
      301 gggttctctc cgagcaggcg gatcatcggc tcctttccca ccgccccaag atcatagatc
      361 acatcgggca ctccatccct gcagcatgat gccacccccc agtccatggt ctttgctcca
      421 gggggctcgg acgagcggtc aaacgagcat atctcgagga gcatctcctc caggattctg
      481 acagtttcgg gggagtaacg gatgttcgcg gcacacctca cctgcgggtc gaaccgcatg
      541 gcggtgagca cgattcttgc gacgtgatcg ctggcaccaa acgagacctg ccccacgggg
      601 tgcgcccgcc ctgaaaggcg cacgatgcgc ccttcgaccg ccgccacctg cgaggggtca
      661 cgcgctgtgg gggtggcgta ggcgatgttc gatcccactt caggtataag gaggacagcc
      721 atgccgagtg agagtgccct tacactctcc tccagttcgc gcagcacaac ctcccgttca
      781 tcgcccggtg tgctcatcat tatcccttgc acctcccggg gtaatttacc tatccttcac
      841 cgtccccggg taacggagtt cccggaagaa gaagataatg atatctgcaa gaacgaagta
      901 acttatgggc atggatgcac gggtactcga tatcctctct gccgtggtca gtttcatcgt
      961 cttgcttgta tttctccttg tgctcccttt gttcctcgaa caggggattg cctacctgct
     1021 ggcgatcgtg atctttatcc tgacgatgag cggggctggt ttctacatca acaaaaccct
     1081 cagttaggct tcgccttttt tcctttcttg cggctccggt agtaattgag ggggtggttc
     1141 actttctcgc agcctgcatc cggcctgacg cagagcccga gggtccgcat cgccgcgcag
     1201 gatggcgcag tatactccgt ccccgtgccg cccctcccgg agatatgctc cacctggtac
     1261 tgggtcttgc tgacgtcgaa gtcgggtgcc ctgcagtaca gttcgattat ctgggcacca
     1321 tccattccga tattgtgcag gaaggccgtg agggcaaaac ggccggagtg cgtgatgttc
     1381 aatccacctg aaagcgccga cataatcgcc tgcatgcagg gcgggaagca cgactcttcg
     1441 acctcgccga agtcttcgag catcttctgc tggaacgcgt cctggatgcg ggaaacatat
     1501 ggcgccatct cttcgcagag ggactgcggg acatcgaggg gcagctggga gaggagtgtc
     1561 acccggatct gctctttcaa cagttcttct gcctcaaggg gnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn ngggagagga gtgtcacccg gatctgctct ttcaacagtt
     1741 cttctgcctc aaggggctcg attgcaacgt ttccatcctc cacctcccga ttcaccagcc
     1801 tccagcgctc ctctctcatc gctgccgtga tctccacgta gcggctgacg gggagggaca
     1861 ggcgcccaag ggagaagccg atctgggatg cgacgtatgc cctcttccca tcatcttctc
     1921 cttcaaggaa ggcgcaggca cgcagggcct cgtaccgcgt gaggcgatcg atcaaggcac
     1981 gttccttccc gcaggagaca aggagcctcg caagcgcata ggatgacacc tcgagcctca
     2041 cgccgagact gtctgaaggg agagggattc gttcaatctc gattgtctgg ccggcatggg
     2101 agagggcgcg ctggaccctc tccactgcgt acctgatagc gacctttccc gcactggact
     2161 ggagaaacct gtcgaccgat tcatggttct gcagcatgaa ctgctgcgat tccttcaaaa
     2221 aagggtattt ggcaaaatct ctcgtctcaa gggagacctg catcaatccg cctcaatgcg
     2281 gggtgcgagc aggtactcca catacccgtg ccccccggcg atatagaacg caaattttgc
     2341 cgggtgatcg atcccaagcg agacttccac ctcctcggcc ctgctcatca cctttcccat
     2401 gtctttgagg taatcgagcg agaagagcga acgggcttcc atcccgttga ggaaattgac
     2461 ctcgttcttc ccgaactctc tcctgatatg gtcggtatcc ccttccgctg ccatgaagaa
     2521 gagctgctgg gccgggtcta tcccgagcgc gatcttgtcg gagacgaccg ctgctgcctt
     2581 gatggagttg ttgaggtcgt tcccggagag gaccactttt ccggggagat cgatgttcgg
     2641 cgggttcggg tctttcctga tggtgtttgg gtcaagcagg gtgatggagt accggtatcc
     2701 ctcgaaactt accttcagct tgtgaccgtc ctctgggagt tccaggctga tgacatcgtt
     2761 ctttcccatc atcccgaaga tattcttcat cttggcgata tcgagcccga tctcgccttt
     2821 cgtagattca aatgcggaaa acgcctcctt cttcagtgtg agggagacca tggccacgtt
     2881 ggcggtatcg accgcccggg tggaaagccc gttctcgtcg gtgtggagcc ggcattccgt
     2941 gaccagggct gagatggcgt cgatggactc tttaagaata tctgcatcaa tcgttgcttt
     3001 taacatcgta caccccgtat atatgtaatt ataaactacg ccatcaacgt tttaatgata
     3061 tctgatttca tccgatcttc ccggtatttc gacggtggat attactatgg gttcacaatg
     3121 gggaaaggat cgcacgtatc tgcgggcagt aagagaggga taccggtcca gggctgcgta
     3181 taagctgcag gagatacagg aacgctatca cctgatccgg gaacatgaca acgtcctcga
     3241 cctgggagct tccccgggga gctggctcca ggttgcacga ggactcacgg cgggaaggat
     3301 cctcgggatc gatctctccc ctattgcccc gctcgaaggt gtcaccacac tgatcggcga
     3361 tctcaccgac cggagcctgc gtgatgaggc aagaaaggtt ctcgggatcg tcaacgtgat
     3421 catctccgat gccgctccga aactctcggg ccaccgaagc tatgaccagg caagggcgat
     3481 cggccttggg caggaggttc tctcgttcgc ctgcgacgtg ctgaagccgg gtggaaacct
     3541 cgtcctcaaa tcgttccagg gagaggactt ccaggcactt ctgaaagaga tgcggcagta
     3601 tttcctctcg gtgaagactt tcaaatgccg ggcttcaagg agaggcagca gcgagatcta
     3661 tatcatcgca aggaatttcg tggggtgcag gaatgaaact gaaggatccc tataaccggc
     3721 cggtcagcaa cctgcgcata agcctgacgc ccgcatgcaa ccttcattgc atctactgcc
     3781 acgcagaggg cgaaaaggat ccgaaggccc ctctctctct ggaggaactt gcagaaatca
     3841 tgaaggttgc ggccaagttc gatatcggca gtgtcaagtt cactggcggg gagccctgcc
     3901 tccgcgctga tcttgccgat atcgtccgcg ccgtcccgga acgcatggag tgctcgatga
     3961 ccacgaacgg aacgctcctt gccgacatgg cacatgatct cgcggatgcg ggtctctcgc
     4021 gggtgaacgt gagccttgac agcctcgatc ccgccaccta catgaagatc acgggctccg
     4081 actgccttga cgacgtgctg gccgggatcg atgcagcgac agaggcccgc cttactccca
     4141 tcaagctgaa catggtgctc ctgaagggga tgaacgatca cgaggttgac gagttcatca
     4201 ggttcgtgcg ggggaacagg gatctcatcc tgcaggtgat agagctgatg gagttccggg
     4261 actgcaccaa ccaccacgag ttaaaagacc tcgaggtatg gcttgccagc aattcgcagg
     4321 agatcctcac ccgccgcatg caccacagga agaagtactg cattgacggt gccgaggtgg
     4381 aggtggtgag acctcttcac aacaccgagt tctgcgccta ttgcaacagg cttcgggtca
     4441 cgtcggatgg aaaactgaag ccctgtcttc tccggaccga caaccacgtg gacatccgcg
     4501 gaaaatacgg cgaggaactc gaggagctct tccgcgaagc ggtccgccga agggcgccat
     4561 tcttccagta aaaggattat tttcgccatt atttcccaaa aaaaggaaac tataccgttt
     4621 ccggaatata tttactcccc cgcgtctgag gatctaccat gagttcggac gctgaatacg
     4681 tgcacgtact tgagcacctg tacgagaagt cccttatcct gcaggacgag accatgtggc
     4741 atcccgtgct tgccttttat tacatggacg cccttgccca tctcgattat gtgatcagcc
     4801 tcatggccta ccactacaag tcgccccggg tgatgatggc cggcgaatac ctgcgttgca
     4861 gggtcgacca ggagaagctc ggcgaccgcc ccaggttccc ggcgttcgtc aactggctga
     4921 agagagaaca ccccaagagg ttcgaggcgc ttccgacggt ctggcggagg gtgtatgacg
     4981 aggacgacga agcgcgctat atcagtttcc ggatagtctt tgaccgggac agcaaggacc
     5041 cgatacgccc gcacgtctat cgctctctca tcgaggagtt tttcaacact gaatttttga
     5101 agactctcta cagtgatgcc tccctttcgg ccctgttcga ggaattcaaa aggaaagcct
     5161 gacccatttt tttatgaatg ttaccgacct ctgcgcagac ctggtgagga tccggagcga
     5221 aaatcctcct gggaatacca gggatgtcat tgaatatatc cgtaatttca ccgattcgct
     5281 tggaattgcc acgcaggtca tccgggggcc gggaggaaag gacaaccttg tctcccgcac
     5341 ggcaaacggg aggcttcttt tctgcgggca cgtcgacgtg gtcccggcac ttgatgaagg
     5401 ctggacccat gatccctttg ccgggactat tgaggacgga aaggtctggg ggcgtgggac
     5461 taccgacatg aaaggcggct gcgcctcggt cctctgggca tgcaggaact ggcttgattc
     5521 cggcagggaa cttcctgcag acctcgcatt cgtctgcgac gaagaaacaa gcggaacgca
     5581 cggtatccag gcactcctcg ccaccaagtg cctggtcccc tgcgatacga tcgtcgccga
     5641 gccgacaccg tcgctctccc caaacgtggg tcagaagggc ttaatgcgcc tctgctgcag
     5701 gttcagggga gagcccgggc acggctctct ctatcctgcg aagggagtga gcgccgtgat
     5761 ggaggcgttc tcgctcctcg agtttgtcag gctgctccac aagcgggaat tcagccccgc
     5821 agatcccgcc ctctccggga ttatcgggga atcgtcaaag atcctgggag agatcttcgg
     5881 aatagaggac acgaaaagag tcctgtccca cgtgatgtac aaccccggga agatcgaggg
     5941 cggcgagaag gcaaacatcg tagcccagca gtgcagcctc gagcttgacc tccgcatacc
     6001 ctgggggtgc agccttcctg atctcctgga ggagatccgc cgtcatgccc cccgcggaga
     6061 aatatcagtg accaatagct cggatcctac catcacgcct ccggactcgc cgctcgtgac
     6121 ccgcctcctt cgggagatag agaaggtcca ctctgccacc gcccggccca tcgtccagtg
     6181 ggcggcaagc gacgcccggt acttgcgaaa cgccgggttc cctgtcgttg agtatggtcc
     6241 aggcgagatc cggacactcc atgcaatcga cgagcatgtc accatagagt ccctggagaa
     6301 cgctgccagg gtatacgaag ggatgctcgg ccgttatgcc atgatatgag ggatgcaccc
     6361 tctatgggac tggaataggc tgatttcccg gtacaaatat gaatttattc tgtcgcctct
     6421 atacacaccc gcctccttgc cccattcata tggtttaaca catcgccagc atatcatttc
     6481 attcgtggtg aatgaaatgc cgaacggagg accgggaatg gggatgcagc gaagaggagt
     6541 ccctcctgac gcctgcatct gtccgaaatg caagaaagag tacccaaaga cacgcggggt
     6601 tccctgcagg agccagacct gcccggactg caatattccc ctcatgggca agtaaaaagc
     6661 ggagtagaca tttttttttc tttcagcatt gccgggtgaa ccactcaccc tgtctgtcga
     6721 ttgagtaaaa gccaggaaaa atgtagg
//
LOCUS       DAOQVJ010000013         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_12571_length_6698_cov_4.323348, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000013 DAOQVJ010000000
VERSION     DAOQVJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12571_length_6698_cov_4.323348"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1199
                     /locus_tag="PKL26_00565"
     CDS             108..1199
                     /locus_tag="PKL26_00565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNS82218.1"
                     /translation="MSSDEMDQKTQQRLVSIAMRWYEQMKEGAIPSISLPTRTKQNIE
                     FDDDAEVWKYGDRESIRSAHTAKSALHLLKMSYVIAFLKKQLHERRSSTLRELYYISE
                     GWKRAKFSAQDESNLLIEDLEILTDLQREAFHLRPEEDGATIFGPLRLKEMTRRGERV
                     IHCQEDVGEAGYQIPSNVENIDLLEHDARFVIAIETGGMYARLIENGFDQEHQAILVH
                     LKGQPARSTRRMLHRINEAFGLPVLVFTDGDPWSYRIYASVAYGAIKSAHMSELLATP
                     RAQFIGVQPSDIRDYNLPADTLTDKDIAALQSEISDPRFSSEYWKSQIQLQLKLNLKS
                     EQQAFAARGLDFVTKEYLPRRLTEMGILS"
     gene            complement(1487..2473)
                     /locus_tag="PKL26_00570"
     CDS             complement(1487..2473)
                     /locus_tag="PKL26_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS82219.1"
                     /translation="MDLSQKEIGQFAREQVEITSKLERHRSQRSGWRKVLKHPVQVLL
                     EKPLNILILTLPLAALLFLIGTAFAIRDYGMSVLFTTTLIDDIVIISILVAIVPVAIM
                     DFRESRRMKNLEEALPNFFRDLAGMNDSGMTLPNAIHLVAQGEYSTLTPFIRRLDNEM
                     SWNTTFIEAIQRFRARLGTPLSDRSIDLIAKASKAGGDVSEVLRAAARDTYEVLALRS
                     ERKNNMLIYVIIVLISFMVFVFVILILVSTFLSTMATAGSAASAAGAGAASFIGNVDL
                     FFYTRLFSHAAMIQGIFSGLVAGQMGEARVVAGLKYSAIMLVIAWLVFRFMV"
     gene            complement(2679..3602)
                     /locus_tag="PKL26_00575"
     CDS             complement(2679..3602)
                     /locus_tag="PKL26_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS82220.1"
                     /translation="MNGFERLAFRIMGGYAEKKRDAYSTLRNDLVTARMKVPFEVYLS
                     TAYLSSVIVGIVASIVLGLITYLLNLPAMIKYQGAVPEFMLTINEYSLIIGTVLAIII
                     SFAVIGGITFLIFLIYPSVVAGGRCRNIDATLPYAINYITAMSTAGIPPAEIFRQLGS
                     STMYGESAVEARFIGMEIDLFGRDLIDALRLISATTPSMRMKEFLQGAMGCISAGSSL
                     TEYFRTKAEQYSLENRQTQKLFLDTLGLISESYVTAMVAGPLFLIILQSIMAILSQSS
                     QPIFLYIIIYMMIPFGSIMFVILISSMTPEG"
     gene            complement(3599..5854)
                     /locus_tag="PKL26_00580"
     CDS             complement(3599..5854)
                     /locus_tag="PKL26_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNS82221.1"
                     /translation="MTAEDKKGLSFRDLFGRKKGEGPADKPKKEGTQEKNEVSVPPVE
                     REDLDGFMKRLGLSLDTKKTPSPGPQEKVPSGIEPGEEMPPVEVVEKPLAGLDSQKKA
                     LDISAPTEIPPIEKKETVTDRSPPRFEPVKKTVWSEKKPVLEETVISVDQINDLSGLI
                     LPKGATFQIEEIKIQDRSNIFSFKGESGAVAGKDLQKTDLRDSSLKKVSDAAIEVHEE
                     AGKGGLLARLNPINVIHRVPVEYNPKTHGPLVDLTFRPRPGIEQTELYPVNEPYAYVR
                     ITYDNATHEYLYEVLEPKLTPAEVDLFRDIKERIFERLDINTQNVLEEIAKKTLRDVT
                     DDIIHDYGLSLSPVQREKILYTMYKEFLGNGMIDPLMHDRYIEDISCDGVNANLFVYH
                     NRYESMRTNLLYKSSDELDSFVTKLAQRAGKYISIAEPMLDATMSDGSRIQMTLGTEV
                     TAHGSTYTIRKFKDEPITPTDLIEWRTFSPLSIAFFWMAVESGKSCIFAGGTASGKTT
                     SLNAISLFMPPLAKIVTLEDTRELKLPHPNWIPSITRTSFDTSGKGEIDMYELLRAAL
                     RQRPEYILVGEVRGKEALTLFQAMSTGHVTYSTIHADSVASIVHRLENPPLNVPRNML
                     SALDLVSVQVQARIGGQRIRRNKQIIEILDIDPRTNELITNEVFRWHPATDEITYSGK
                     SYVLEEIMESRGWDEDRMREELKMRQEVLEWMRIKKIRNFRDVGNILVQYYRDPAGTM
                     EKIRAELYGGA"
     gene            complement(5919..6617)
                     /locus_tag="PKL26_00585"
     CDS             complement(5919..6617)
                     /locus_tag="PKL26_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS82222.1"
                     /translation="MSLPEGTYAGVSSDPVQEDRPGGASPFTGKVEILTDEGAGLLIV
                     DNGRVDAAYYHDNSGDYRGKDAVVHLQHRSEEAGLSIRYIQYRYSPGQASDAIQICRR
                     EGLSLFEEDTHAKKPVRAGLDDALIARLSGQPGVRAVSIFFEGFPVQSVGDGDFEQVA
                     AMAEDFLRAGRKMAENIRMGVPDQITLESDQGKCIIAPYGDLFLCLLTDSDANIGLIR
                     LAIRTIQKELKGRV"
BASE COUNT         1478 a         1837 c         1834 g         1549 t
ORIGIN      
        1 ggagcttctt cccgggggcg gatggactgc agtatacagc ggttcagggg gagggagcct
       61 cgaagtacgg ggaatacccc cggaacacgt agtggtggtg gatctcgatg tccagtgacg
      121 agatggacca gaagacgcaa cagcgcctgg tgtctatagc catgcggtgg tacgagcaga
      181 tgaaggaggg agcgatcccg tccatctcgc tcccgaccag gaccaagcag aatatcgagt
      241 ttgacgacga tgcagaggta tggaagtatg gtgaccgcga aagcatacgc tcggcacaca
      301 ctgcaaagag tgcgctccac ctcctgaaaa tgtcctacgt gattgccttc ctgaaaaaac
      361 agctccacga gagacgctca tcgacattga gagagctcta ttacatctcg gaagggtgga
      421 aacgggccaa gttctccgcg caggacgaga gcaacctgct gatagaggat ctcgagatcc
      481 tcacagacct ccagcgggag gcattccatc tcaggcccga ggaggacggt gccaccatat
      541 tcggcccgct cagactaaaa gagatgacga ggagagggga gcgggtgatc cattgccagg
      601 aggacgtcgg cgaggcaggg taccagattc caagcaacgt ggagaatatt gatctccttg
      661 agcacgatgc tcgctttgtc attgcgatag agaccggtgg tatgtatgcc cggctgatag
      721 agaacgggtt cgaccaggaa caccaggcga tccttgtaca cctgaaaggt cagcctgccc
      781 gctccacccg ccggatgctc caccggatca acgaggcgtt cggcctgcct gtgctggtct
      841 ttaccgatgg cgatccctgg tcataccgga tatacgcaag cgttgcttat ggtgccatca
      901 agagcgccca catgtccgag ctccttgcca cgccacgtgc ccagttcatc ggggtgcaac
      961 ccagcgatat ccgggactac aaccttcccg cagataccct caccgacaag gacattgccg
     1021 ctctgcagag cgagatctcc gacccgaggt tctcttcaga atactggaag tcccagatcc
     1081 aactgcagct gaaactcaac ctcaaatccg aacagcaggc atttgcagcc cgtggtctcg
     1141 actttgtgac aaaggagtac ctgccccgcc ggctgacaga gatgggtatt ctctcctgat
     1201 aaaaacccca tgattctcat tgagacacat gaatgcagaa taaaaatggc cgagccattt
     1261 tcatagtaac cgctcatgac tgccattcca tcgaatatac acgatgaaaa tcacgaagtg
     1321 attttcattc gaaaaaagag agggacgctc ccccacacgc tccgatgtga gcctcctgtt
     1381 atctggtctt aaggaatatt ttctccggtc tgaattggca ttcacaccag ggtgagaaga
     1441 aaattgttcc tgtattgggg accacgcgtc cctcacacaa ttattctcat accatgaacc
     1501 tgaagaccag ccatgcgatg acaagcatga tcgcagagta tttcaggccg gcaaccaccc
     1561 tcgcttcacc catctggccg gcaaccagcc ccgagaagat gccctggatc attgcagcgt
     1621 gtgagaagag ccttgtatag aagaagaggt ctacgtttcc gatgaatgat gccgcacccg
     1681 ctcctgccgc ggatgccgcg gagcctgcag tcgccatggt cgagaggaac gtactcacca
     1741 ggatcagtat cacaaagaca aagaccatga aagagatgag gacgatgatc acgtagatca
     1801 gcatgttgtt ctttcgttcc gaacgaaggg caagcacctc gtaggtatca cgggctgcag
     1861 ccctgagcac ctcgctcaca tctccgcctg ccttgctggc cttggcgatg aggtcgatac
     1921 tcctgtcaga gagcggagtg cccaggcgtg cgcggaaacg ctggatggcc tcaatgaatg
     1981 tcgtattcca cgacatctcg ttgtcaaggc gcctgataaa cggggtgagg gtgctgtatt
     2041 ccccctgtgc aacaaggtga attgcattcg ggagcgtcat tccgctgtca ttcattcccg
     2101 cgaggtcacg gaagaaattc gggagggctt cctcgaggtt cttcatccgc cggctctccc
     2161 ggaagtccat gatcgcaacg ggaacaatcg caacgaggat ggaaatgatg acaatatcgt
     2221 ctatgagggt cgttgtgaag agcacgctca tcccgtagtc ccttatggca aaggctgtgc
     2281 cgatcaaaaa aaggagggcg gcaagcggca gtgtcaggat caggatgttg agcggttttt
     2341 cgaggagcac ctggacaggg tgcttgagca ctttacgcca gcccgaccgc tggctgcggt
     2401 gccgctcaag tttgctggta atttctacct gctcccttgc aaactgtccg atctccttct
     2461 ggctgagatc cacggaatag ggtttttctc ccttcaatat cccccataac ccgggccttt
     2521 ttgcaggccc gcctgccgga ggaagaggtt ttcgtctggg cgacgaagga gatacggggg
     2581 gcacagctgg cgatgctggc acctctgctt cactcccgct ggcagatcca gaaccttttt
     2641 ttggcagcat ccccttgaaa gagtctttga ggcccatctc aaccctccgg agtcatggaa
     2701 ctgatcagga tcacgaacat gatgctccca aaggggatca tcatgtaaat gatgatgtag
     2761 agaaagatcg gctgcgacga ctggctcagg atggccatga ttgactggag gatgataagg
     2821 aagagggggc ctgcaaccat ggccgtgaca tacgactcgg atatcagccc gagcgtatca
     2881 aggaagagct tctgggtctg gcggttctcg agggagtact gctctgcctt ggtgcggaaa
     2941 tactcggtga ggctgcttcc cgccgaaatg caccccatcg ccccctgaag gaactccttc
     3001 atgcgcatgc tcggggtggt cgcagagatg agccggagcg catcgatgag gtcccgccca
     3061 aagagatcga tctccatccc gatgaaacgg gcctcgactg cactctcacc gtacatcgta
     3121 ctgctcccca gctgccggaa gatctctgcg gggggaattc ccgcggtcga cattgcggta
     3181 atatagttga tggcatacgg aagcgtggcg tcgatgttcc tgcaacggcc tccggcgacc
     3241 acgcttgggt atatgagaaa tatgaggaat gtaatacccc cgatcaccgc aaacgagatg
     3301 attatggcaa gtacagtgcc gatgatcagg ctgtactcgt tgatcgtgag catgaactcc
     3361 ggaaccgcac cctgatactt gatcatcgca gggagattca ggaggtaagt gatcagcccg
     3421 agcacgattg atgcgacgat acctacgatg accgaactta agtatgccgt cgagagatag
     3481 acctcgaacg gcaccttcat gcgcgcggtg acaaggtcat tccggagcgt ggaatacgca
     3541 tccctcttct tctccgcgta accccccatt atcctgaatg cgagacgttc aaacccgttc
     3601 atgctccccc atacagttcg gccctgatct tctccatggt tcctgcaggg tcacggtagt
     3661 actggacaag aatattgcca acgtccctga agttgcgtat cttcttgatg cgcatccatt
     3721 ccagcacctc ctgccgcatc ttgagctcct ccctcatccg gtcctcatcc cacccccgcg
     3781 attccatgat ctcctcgagg acgtaggact tcccggaata ggtgatctcg tcggtcgccg
     3841 ggtgccacct gaagacctcg tttgtaatga gctcgttggt gcggggatcg atatcaagta
     3901 tctcgataat ctgcttgttt ctccggatgc gctggccacc tatccttgcc tggacctgga
     3961 ctgacacgag gtcaagcgcg gagagcatgt tcctcgggac gttcaggggc gggttttcga
     4021 gacggtgaac gatgctggcc acggagtctg cgtggatggt cgagtaggtc acgtggcccg
     4081 tactcattgc ctggaagaga gtcagcgctt cctttccacg gacctctccg acaaggatat
     4141 attcggggcg ctgcctgagt gcggcacgga ggagctcgta catgtcgatc tcccctttcc
     4201 cgctcgtgtc aaacgaggtc cgcgtgatac tcgggatcca gttgggatgg gggagcttga
     4261 gctcgcgtgt atcctcaaga gtgacaatct ttgccagggg gggcataaag agggagatgg
     4321 cattgagcga ggtcgtcttt ccagacgcgg ttccccctgc aaagatgcag gacttgccgc
     4381 tctccacggc catccagaag aaggcgattg agagggggga gaatgtgcgc cactcgataa
     4441 ggtctgtggg agtgatcggc tcatccttga acttcctgat cgtgtaagtg gacccgtgag
     4501 cagtcacctc agtgccgagt gtcatctgga tacgggagcc gtcactcatg gtggcatcaa
     4561 gcatcggttc cgcgatagag atgtacttac cggcacgctg ggcaagcttg gtcacaaacg
     4621 agtcgagttc atccgagctc ttatacagga ggttcgtacg catcgactcg tagcggttgt
     4681 ggtagacgaa caggttggcg tttacgccat cgcaggagat atcctcgata taccggtcgt
     4741 gcatgagtgg gtctatcatc ccgtttccga ggaattcctt gtacatggtg tagaggatct
     4801 tttccctctg gaccggcgag agcgagagcc cgtagtcatg gataatgtcg tcggtaacat
     4861 cgcggagcgt cttctttgcg atctcctcga gcacattctg ggtattgatg tcaaggcgtt
     4921 cgaatatccg ttccttgatg tccctgaaca gatcaacctc ggcaggagtc agttttggtt
     4981 caaggacttc gtacaggtat tcatgggttg cgttatcata ggtgatgcgg acatacgcat
     5041 acggctcatt cacagggtat agctctgtct gttcaatccc gggcctcggc cggaatgtaa
     5101 ggtccacgag aggaccgtgg gtcttcggat tatactcgac cggcactcta tggatgacat
     5161 ttattggatt gagccttgca aggagtccgc cctttccggc ctcctcgtgg acctcgattg
     5221 cggcgtcaga tactttcttg agactggaat cacgaagatc ggtcttctgg agatccttac
     5281 ccgcaaccgc accgctttct cccttgaacg agaagatatt gctgcggtcc tggatcttga
     5341 tctcctctat ctggaatgtg gccccctttg ggaggatcag cccggagaga tcgttgatct
     5401 ggtcgacgga tatcactgtc tcttcaagaa ccggcttctt ctctgaccaa acggtctttt
     5461 ttacaggctc aaagcgtgga gggcttcgat cggtgacggt ctcttttttt tcaatcgggg
     5521 ggatttctgt gggggcggag atatccaggg cttttttttg ggagtccaga ccggcaaggg
     5581 gtttttccac cacctcaacg ggcggcatct cctctccggg ttcaatgccg gagggaacct
     5641 tctcctgcgg cccgggagag ggggttttct ttgtgtcaag ggacaggccg agacgcttca
     5701 tgaagccgtc aaggtcttcg cgttctactg gaggaaccga tacctcattc ttctcctgcg
     5761 tcccctcttt tttgggttta tcggcagggc cttctccctt ctttcttcca aaaaggtctc
     5821 tgaatgaaag gcccttttta tcttctgcag tcatcgctga attccacttc tcctggagtt
     5881 atcccacgtg aggattctct cgcgaagtat cccccttctt ataccctccc cttcagttcc
     5941 ttctgaatgg tacgtatggc aagccttata aggccgatgt tcgcgtccga atccgtcaga
     6001 aggcaaagga agaggtctcc gtatggtgcg ataatacact ttccctggtc gctttcaaga
     6061 gttatctggt cgggcactcc catgcggata ttctctgcca tcttcctgcc agcccgtaaa
     6121 aaatcctctg ccatggctgc gacctgttca aagtcgccgt cgccgacaga ctggacgggg
     6181 aacccctcga agaagataga gactgcacga actcctggtt gccctgacaa tcgtgcgata
     6241 agagcatcgt cgagtcctgc cctgaccggt tttttggcat gcgtatcctc ctcgaacaag
     6301 gacaatccct ccctcctgca gatctggatc gcatcactgg cctgaccggg ggaatacctg
     6361 tactggatat aacggatact aagtcctgcc tcttcagagc gatgctgcag atgtaccact
     6421 gcatcctttc cccgataatc cccggaatta tcgtggtaat aggcagcgtc gacccgtcca
     6481 ttatccacga tgaggagacc tgccccttcg tccgtcagga tctcaacttt tcctgtaaaa
     6541 ggactcgctc ccccggggcg gtcctcctgg acagggtcag acgaaacacc cgcatacgta
     6601 ccttcgggga gactcatgcc ttataacgct gcaacaaggc gttctttgtt tttctttaat
     6661 tcgtaacgga ttcatccaat gtttgcctcg tttccggc
//
LOCUS       DAOQVJ010000014         6670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_12637_length_6670_cov_3.794407, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000014 DAOQVJ010000000
VERSION     DAOQVJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12637_length_6670_cov_3.794407"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PKL26_00590"
     CDS             <1..1639
                     /locus_tag="PKL26_00590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNS82223.1"
                     /translation="QRAERGSLVPSRDPYIQQQIRRMDPQRPLLCPHFIGSRVFLQSE
                     GGLRMVPSPALQTKAERVTSQTVWPHQLPEVASGVCPYEMMILAAQKADVILLNYRHL
                     FDEDIREALYASLALDPADAILLVDEAHNCGDAVTAAQSVDLDQDTLTLAGTELSGLR
                     RAKAGVEALQQVIPRITGFMEGLQNSFETEDWFDPAIFDRMVVKGSLYHSMAGIVDDL
                     EEISGKLREKNIKAGEFRETALERLTRFLQKIAASSKDPASLTLFRKEDGLVSLEVRS
                     IDPGPPLQEIARSHHACLLISGTLSPVEAYARFYFGDLPARTLSLPNAFPKENRLVVC
                     ASDITTAFSMRQNEKNLQLIRAYIEAFSRLDGNLAVYFPSYQILEGFSKDLTTAGRKK
                     KKIFVEPRDAKDAEESLREFLSLPGSGRSGLLLAVCGGKWSEGLDYRGDLLQGAMVIG
                     LPLAPFNQVRRMVIGYFRRRFGDEGEFLCYTLPAINRGQQALGRVLRTPDDRGVLVLG
                     EKRFLEGKVSRGLPEWMRNEMRSCTLPEFEDLVGRWR"
     gene            1630..2073
                     /locus_tag="PKL26_00595"
     CDS             1630..2073
                     /locus_tag="PKL26_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNS82224.1"
                     /translation="MEMREGSCRFGLWHVHVHWSKDTVRLVRFSTRPLGGDVPLPIRR
                     YCAGFPEGLHELASIALSPEYPFSAIYRRVREIPYGNTETYGEIARDLNTSPRLVGLA
                     MKRNPTPLVIPCHRVVSKTGTGGFSPDPAIKEALLAMERRNTRCL"
     gene            2241..2825
                     /locus_tag="PKL26_00600"
     CDS             2241..2825
                     /locus_tag="PKL26_00600"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNS82225.1"
                     /translation="MDRSAVILVGGEAKRANGLEKYFFTFGGKTFIERLTDTLAEVVD
                     EIILVAKDPAQCSRFSHLDNVRCVTDIRRGYGPIGGLHAGALAAKGDALFVCACDMPC
                     VSTQVVESLFSLLEGYDAVIPQWATDMIEPLHAVYRRSALLSALESHESLSLRRMVKG
                     IRTRYVDVAGIRELDPELRTFININKIEDLGKLS"
     gene            complement(2820..3512)
                     /locus_tag="PKL26_00605"
     CDS             complement(2820..3512)
                     /locus_tag="PKL26_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82226.1"
                     /translation="MKLLGISPALAVVLAVCLLFSGCITPPKETPPGPMTQNGGMATT
                     VPTPSPTQEIMDTQYVTPATPFPTGTPATTRPTYSSTPNFTFEPTVYKVIYLNTIDFK
                     YSVSAYSTTVENPPLIIEVCLAPKMVTRNIWYESRYTSREDVYTTQTYVSPASYFEIR
                     VRDKASGDIVEKEGFGKTYSVDTKKVLQVRTSGEYLVEFSGNDIVATVQMRVPAPAGA
                     DVTPAGTLACPS"
     gene            3643..4068
                     /locus_tag="PKL26_00610"
     CDS             3643..4068
                     /locus_tag="PKL26_00610"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82227.1"
                     /translation="MVIPGLAAMEKKSQFWKKTDKSLNTADGYVLKADLLAHDQKFEE
                     AVEYYDKALEIVPKNADVWAFKGITLQGGLGRDEEARACWEKAKALDHDIAMAVDMTK
                     EEPARGEDLESIRWSDLHDSCREKLKRMMLKNVDAGTRK"
     gene            complement(4162..4344)
                     /locus_tag="PKL26_00615"
     CDS             complement(4162..4344)
                     /locus_tag="PKL26_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82228.1"
                     /translation="MVDFTAYLVISIVTLCFGLLVSFQVAHIMFVRRLTRLAKRCVDT
                     GTIAPLLVELEIAHKK"
     gene            complement(4370..4558)
                     /locus_tag="PKL26_00620"
     CDS             complement(4370..4558)
                     /locus_tag="PKL26_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82229.1"
                     /translation="MSIVMEDVSVYLVIGVLMFCIGLMAGYLLMTTYYSRRFLSVAKE
                     CERADSIAPLITEMERES"
     gene            complement(4648..5232)
                     /locus_tag="PKL26_00625"
     CDS             complement(4648..5232)
                     /locus_tag="PKL26_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82230.1"
                     /translation="MNPDISLHPAVHEVEFWKRYRALLRMTRHLAGGERLIRALQEET
                     AIPEKTRDEAIGPLKEEHAQNFSAFHDFLVNFVSLALQGLHRADISLEFSFTEGVPRC
                     HRALLHVDGHPRDLPVEEGRRLLACLPLTGEDPHPEQSLLRFYEAMEQRFDRDQKGEL
                     DRCSLEIRQEIYPGSAFHARLHLPAQVFIEGISR"
     gene            complement(5237..5494)
                     /locus_tag="PKL26_00630"
     CDS             complement(5237..5494)
                     /locus_tag="PKL26_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82231.1"
                     /translation="MDLMNIRPWIFFYLICFVALLLATTFIVQGITPLFWMSSTLIIV
                     VIGFNFICIFSEIKEHRKRKALMKQISSGNGIRPVGKDGRK"
     gene            5637..>6670
                     /locus_tag="PKL26_00635"
     CDS             5637..>6670
                     /locus_tag="PKL26_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS82232.1"
                     /translation="MAAHSRILASASQTLDERRDTVKLCIIGVFAVTSLIFTIYLVAS
                     RDQAFSASVYPLIPHLYLIPLILMALWYPRRGLQITILLIAAILLLTTFMYLMGAIIN
                     PFIALLNAGMDIAIFVALALYAKDRTLVESFLKGFFEHSGMGESFEEAMEHPSLRAKI
                     KFEGAFDDVIRALHSQDEEMREEAAKALGELGDFRAVDPLIELLADDNRYVRREAAKS
                     LGKIGDEKAIPALINALKDEDRYGREGAAEGLGEMGGHAYPSLIDAMADDDWHVRMGA
                     AIALRIIGDRDALPVLITAMQDENRFVRREVIKSLGRIGDHSVIDTLIEALKDPDRSV
                     RLRAVSALSK"
BASE COUNT         1359 a         1979 c         2002 g         1330 t
ORIGIN      
        1 ccagcgagcc gagagggggt cgctcgtgcc ttccagggac ccgtacatcc agcagcagat
       61 acggcggatg gacccccaaa ggcccctgct ctgcccccac ttcatcggga gccgcgtctt
      121 tctccagtcg gagggcggcc tgcgcatggt cccctctcct gcactccaga caaaggccga
      181 gagagtcacc agccagacag tatggcccca ccagctgccg gaagtggcct caggtgtctg
      241 cccctacgag atgatgatac tcgcagccca gaaggctgac gtgatcctgc tgaattaccg
      301 gcacctcttt gacgaggata tcagggaagc cctgtacgca agcctcgccc tcgacccggc
      361 cgatgccata ctgctcgtcg acgaagcgca taactgcggt gatgcggtca cggccgccca
      421 gagcgtggat ctggatcagg acacgctcac ccttgccggt accgaactct ccgggctacg
      481 ccgcgcgaag gcgggagtcg aggccctcca gcaggtgatc ccgcgcatca cggggttcat
      541 ggaaggactg cagaactcct ttgagaccga ggactggttc gacccggcaa tctttgacag
      601 gatggtagtg aagggatcgc tttaccattc aatggcaggg atcgtggacg acctggagga
      661 gatatcggga aagttgcggg agaagaatat caaggccggg gagttccggg agactgcgct
      721 cgagcgcctc acccggttcc ttcaaaagat tgccgcatcc tcgaaagatc ccgcctcgct
      781 caccctgttc aggaaggagg acggtctcgt ctcccttgag gttcggagca tagacccggg
      841 gcctccgctc caggagatcg cacgttccca ccacgcatgc ctgctcatct ccgggacgct
      901 ctcccctgtc gaggcatacg ccaggtttta cttcggcgac ctccctgcaa ggactctctc
      961 ccttccgaat gccttcccga aagagaatcg ccttgtcgtg tgtgccagcg acatcaccac
     1021 cgccttctcc atgcggcaga acgagaagaa cctccagctg atacgcgcat acatcgaggc
     1081 attctcccgc ctggacggaa acctcgccgt ctacttccca tcgtaccaga tcctggaggg
     1141 attctcaaag gatttgacaa cggcaggcag gaaaaagaaa aaaatcttcg tggagcccag
     1201 ggacgcaaaa gatgcagaag aatccctacg cgagttcctc tctctccctg gttccgggag
     1261 atccgggctt ctcctcgctg tctgcggggg gaagtggagc gaaggcctcg attaccgcgg
     1321 ggatctcctg cagggcgcga tggtgatcgg gcttcccctt gctcccttca accaggtccg
     1381 aaggatggtg atcgggtatt tccgccggag gttcggggat gagggggagt tcctgtgtta
     1441 caccctcccg gccatcaacc gcggccagca ggcactcggg agggtgctcc ggactcccga
     1501 cgaccgggga gttctcgtcc tgggggagaa acggttcctt gaggggaagg tctccagggg
     1561 tctcccggaa tggatgagaa acgagatgcg ttcgtgcacc ctcccggagt tcgaggatct
     1621 ggtcgggagg tggagatgag agaggggtca tgccggttcg ggctctggca cgtgcacgtc
     1681 cactggtcaa aggacaccgt gaggctggtc cggttctcga cccggcctct cgggggagac
     1741 gtgccgctcc cgatcaggag atactgcgcc ggcttcccgg aaggactgca tgaactggca
     1801 agtattgccc tttctcccga atacccgttc tcggccatct accggcgggt aagggagata
     1861 ccgtacggga acacggagac gtatggcgag atcgcgcgcg acctcaacac ctccccccgc
     1921 ctcgtgggcc tggcgatgaa gcgaaacccc accccactcg tcattccctg ccacagggtc
     1981 gtctcgaaaa ctgggacagg gggattttct cccgaccccg ccatcaagga ggcgctcctt
     2041 gccatggagc ggcgaaacac aaggtgttta tgaggataag gcccagtctt ggaggaaagg
     2101 cgtcccatga cttcgcgtgt cccgatggtc ttcgaacgcg tcatttcaac cgcggcatct
     2161 ttcatgagcc ctggccagca gaccgcgacc cgtgggatgt ctccgcatcg atacgacctg
     2221 gtgctgcctg gagggccctg atggaccgct ctgcggtgat ccttgtgggc ggcgaggcta
     2281 agcgcgccaa cggtcttgag aagtacttct tcacgttcgg gggaaagacg ttcatcgaga
     2341 ggctgaccga cacccttgca gaggtggttg atgagatcat ccttgtcgca aaggaccctg
     2401 cccagtgcag ccgtttttcc cacctggaca atgtccggtg cgtgaccgac atcaggaggg
     2461 ggtacggccc cattgggggg cttcacgccg gggcacttgc cgcgaaggga gatgccctct
     2521 ttgtctgtgc gtgcgacatg ccctgcgtct ccacgcaggt ggttgagagt ctcttctcac
     2581 tcctcgaagg gtacgacgcg gtcatcccgc aatgggcgac ggacatgatc gagccgctac
     2641 acgcggtata ccggcgcagt gccctgctca gcgcactcga gagccacgag tccctctccc
     2701 tccgtagaat ggtgaaaggg attcgcaccc gctatgtgga tgtggcaggc atccgggaac
     2761 tcgacccgga gctcaggaca ttcatcaaca tcaacaagat agaggacctg ggaaagctct
     2821 catgaggggc aggcaagggt tcccgcgggg gtgacgtctg cacctgccgg ggcagggacc
     2881 cgcatctgga cggtggccac gatatcgttc cccgagaact cgaccagata ctctcccgat
     2941 gtccgtacct ggaggacctt ctttgtatcc acgctatagg tcttcccaaa cccttccttc
     3001 tcgacaatgt ctccggacgc cttgtccctg accctgatct cgaagtacga cgccgggctt
     3061 acatacgtct gggtggtata gacatcctcc ctcgaggtat acctgctctc gtaccagata
     3121 ttcctcgtca ccatcttcgg ggcgaggcat acttcgatga tcagcggggg attctccacg
     3181 gtcgtggaat aggcggagac gctgtacttg aaatcgatgg tgttcaggta gatgaccttg
     3241 tagaccgtgg gttcgaatgt gaaattcggc gtgctgctgt aggtggggcg ggtggttgct
     3301 ggcgttccgg tgggaaaagg cgtcgccggc gtcacgtatt gcgtgtccat tatctcctgg
     3361 gtcgggctcg gggtcggaac ggtcgtcgcc attcctccat tctgggtcat gggtccggga
     3421 ggggtctctt tgggcggggt gatgcacccg gagaagagga ggcagacggc gaggacaacc
     3481 gcgagtgcag gagatatccc cagaagcttc attcaatctg ggttgaatgc ccgggtattt
     3541 attagtttat cctgcgaggc agcgcccgtg tatgccaggg acctttccgt tctttccctt
     3601 ccggctgcca tacccttata taaatcccgg tggcataggt caatggttat ccccgggttg
     3661 gctgcaatgg agaagaagtc ccagttctgg aagaagaccg acaagtcgct caataccgca
     3721 gacgggtatg tcctgaaagc cgatctcctg gcccatgacc agaagttcga ggaggccgtg
     3781 gaatactacg acaaggccct cgagatcgtg ccaaaaaacg ccgatgtatg ggcattcaag
     3841 ggaatcaccc tccagggggg cctcgggcgg gacgaggagg cacgggcgtg ctgggagaag
     3901 gcaaaggcgc tcgaccacga catcgccatg gccgttgaca tgacaaaaga ggagccggca
     3961 agaggcgagg accttgagtc gatccgctgg tccgacctcc atgacagctg cagggagaaa
     4021 ctgaagcgga tgatgctgaa gaacgtcgat gcagggacac gcaagtgatg cgtctacaga
     4081 cctggtaacc accgagtcgc caaattttcc cgggaccgga aattcgaagc cccaatcagg
     4141 attattctgc gaatatccgt atcatttctt atgggctatt tccaactcta ccagcagcgg
     4201 ggcgatcgtg ccggtatcga cgcacctctt tgcaaggcgg gtgagccttc ggacaaacat
     4261 gatatgtgcg acctggaaac tgaccaggag gccgaaacag agcgtgacga tgctgatgac
     4321 gaggtatgcg gtgaaatcta ccattgcctt aacctccctt cgtccccgct catgactccc
     4381 tctccatttc cgtgatcagg ggggcgatgg agtccgcgcg ctcgcattcc ttcgccaccg
     4441 aaagaaaccg cctggaataa taggtggtca tcaacaggta tcctgccatg aggccgatgc
     4501 agaacatcag cacccctatc accaggtaca cgctcacgtc ttccatcact atgctcattg
     4561 actgatacag aaggacgata tataaatttt cctcattctg cccagactgc gggaatttat
     4621 agatgagaga tccctggccg gcatgcccta ccggctgatc ccctcgataa agacctgtgc
     4681 cgggaggtgg agccttgcat ggaatgcaga tcccgggtag atctcctgcc tgatctcaag
     4741 gctgcagcgg tccagttcgc ctttctggtc ccgatcaaac ctctgttcca tggcctcgta
     4801 gaaccgaaga agagattgct ccggatgcgg gtcttcgcct gtcaatggca ggcatgcaag
     4861 gagacgccgg ccctcttcaa cgggaaggtc ccggggatgc ccgtcaacgt gaaggagggc
     4921 cctgtggcac cgcggcaccc cctccgtgaa ggagaactca agggagatgt cggcccggtg
     4981 gaggccctgc aacgccaggc tgacgaaatt gaccaggaaa tcgtggaagg cagaaaagtt
     5041 ctgggcatgt tcttccttca gcgggccaat cgcctcgtcc cttgtcttct cagggatagc
     5101 ggtctcttcc tgcagcgccc tgatgaggcg ctctcctcct gcaaggtgcc tcgtcatccg
     5161 gagcagggcg cggtatcgtt tccagaactc gacctcgtgc acggccgggt gcagcgaaat
     5221 atccgggttc accctctcac ttccgtccgt ctttccccac gggacgaatg ccgttcccgg
     5281 atgagatctg cttcatcagg gctttcctct tcctgtgctc cttgatctcg ctgaatatgc
     5341 agatgaagtt gaaccctatc accacgatga tgagggtgga actcatccag aagaggggag
     5401 tgatgccctg gacgatgaag gtggtggcga gcagcagggc tacaaaacat atgaggtaaa
     5461 agaagatcca ggggcgtatg ttcatcaggt ccatggagcg atgtgatacg tagcctgttg
     5521 cgcctcatat acgtttggga gtggggtcca ttggaaggtc cgtgtcatcc gcgatcttca
     5581 tgctccccac aataccgcgt cgtttaaatg gtaccccacg acatagtgaa tgtaccatgg
     5641 cggcccactc ccgcatcctg gcttctgcat cccagacgct cgatgagagg cgggacaccg
     5701 tgaaactgtg tatcatcggg gtttttgcgg tcacttcgct gatcttcacc atttacctgg
     5761 tggcctcccg cgaccaggcg ttctcggcct ctgtctaccc gctcatcccc cacctgtacc
     5821 tcatccctct catcctgatg gccctctggt atccccgccg cgggctgcag atcaccatcc
     5881 ttctgatcgc ggccatcctc ctcctcacca ccttcatgta cctgatgggc gccatcatca
     5941 accccttcat cgcgctcctc aacgcaggga tggacatcgc gatattcgtg gcacttgcgt
     6001 tgtatgcaaa ggaccggacc ctcgtggaat cgttcctgaa ggggttcttc gagcactccg
     6061 ggatggggga gagtttcgag gaggcgatgg agcacccgtc gctccgggca aagatcaagt
     6121 tcgagggcgc ctttgacgac gtgatacggg cccttcacag ccaggacgag gagatgcggg
     6181 aggaggccgc gaaggcgctc ggcgaactcg gcgactttcg ggcggttgac cccctgatcg
     6241 agctcctcgc ggacgacaac cggtacgtgc ggagggaggc ggcaaagtcg ctcgggaaga
     6301 ttggcgacga gaaggccatc cctgccctga tcaacgccct caaggacgag gaccggtacg
     6361 ggcgcgaggg tgcggcggaa gggctcggag agatgggagg acatgcctac ccctcgctga
     6421 tcgatgccat ggcagacgac gactggcacg tccggatggg agcggcgatt gcgcttcgca
     6481 tcatcgggga cagggacgcc cttcccgtgc tcatcaccgc catgcaggac gagaaccggt
     6541 tcgtccgcag ggaggtcatc aagtccctcg ggcgaatagg cgaccacagc gtcatcgaca
     6601 cgctcatcga ggccttgaaa gacccagaca ggagcgtccg cctccgggcc gtcagcgccc
     6661 tctcgaagtg
//
LOCUS       DAOQVJ010000015         6629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_12742_length_6629_cov_3.339367, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000015 DAOQVJ010000000
VERSION     DAOQVJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12742_length_6629_cov_3.339367"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKL26_00640"
     CDS             <1..270
                     /locus_tag="PKL26_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS82233.1"
                     /translation="GSTSGLIKMMTNLGSSLGVALVMLVATAAMGPALAHSSAHTLPP
                     VELAGAFHVAFLFLMCIEILGILLMLPVKEKESGYSTEGEPVTAF"
     gene            complement(281..796)
                     /locus_tag="PKL26_00645"
     CDS             complement(281..796)
                     /locus_tag="PKL26_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82234.1"
                     /translation="MFKTIAHIYDSDDPSPGASRELTERAEEVICSAVLDTRKPLQAA
                     GDYLLDIRIPAPDYTFQRETDISSAIRAFFLKRADEIHRERRLSGRVGLREFQLTIAV
                     CVPALLGIVACDPFPHEPLAIILQNVLLIFCWVVIWQPFQSLVFDRWTLAEKEKVYRQ
                     IAGMEMRVLPA"
     gene            1017..2000
                     /locus_tag="PKL26_00650"
     CDS             1017..2000
                     /locus_tag="PKL26_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNS82235.1"
                     /translation="MTGGGIGSSVSGATEGGDGHAYPEIEKRYTHVRARIHRLFDEPP
                     GDDRLAFGIQAFLAAVVLLNTVTVVIYTVPWLEEEYWLPLNGIMTACLLVFTIEYVVR
                     LWACTSAPTLAARTTYRIQYASSFYMIIDLVSIVPLVFPLFFPRDFALLRAFRLLSIF
                     KLGRYARKSQSLALLKRVVMRKREIFTLMVFFLVFVILFSSTIMYLVEHQAQPEKFSS
                     IPAAIWWSMMTVTTVGYGDIYPVTPLGQSIASLFTLVGVLLLALPSAILASGFIEERH
                     KQGESGVGIPVEQEIELLEKVGHLHEKGVISEEELEDYRHTIQILAGKRGR"
     gene            2269..4269
                     /locus_tag="PKL26_00655"
     CDS             2269..4269
                     /locus_tag="PKL26_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS82236.1"
                     /translation="MIEVDVFLFDILIVLGLSILVLFLGNKFRVPGIVGFILAGMLAG
                     PYGLGLIQDQEIIQFLAEIGVIFLLFSIGMQFSFRTLYHMKKIVLLGGALQVGFTILV
                     TAGLAYMLGFPLPSAIFLGFLICHTSTTITLRVFQDRGELDSPHSRTTLGISLFQDLM
                     SVPMILLLPALSGHDIQPGEFAITVEVALVASLVLMLVLLFYLLPRLMDTITRTRSQE
                     LFLLTILFVCLAITWITSSIGLSLALGAFLAGLLLAETPYFHQAFATILPFRDIFTSF
                     FFISIGMLLNAHILITHPLIVLGLIAGAIILKAVIAGLSSLSLGQSLRTSVLAGIALA
                     NIGEFSFVLILAGMEFAFLSDFSTQVFLTVAVWTMALSPFLISAGPRISGWICDLPLP
                     QRIKAGSEPPPGTLPATHRDHLVIVGFGPNGRNLARVAEMEGIPYIVIDMNPDTVAEE
                     RKKGLPIFFGDAANPSILHHSGIEHARVLAIVTNDPFSSRTITAMAREMNPGIHILIR
                     TRFLRDIDQLREIGADEVIVDEFETSIEIISRVMKRYLAPRVTIDRATSRIREDMYRV
                     FRQPPSDRGIFSEIALRIPQVEVQTFPQPGKSPMAGRTLREQKVRTEFGVSVIAIGRN
                     EETVPNPDGGFRVDAGDRLVVIGKPDQLASFAAVVEMDGTGA"
     gene            complement(4512..5705)
                     /locus_tag="PKL26_00660"
     CDS             complement(4512..5705)
                     /locus_tag="PKL26_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS82237.1"
                     /translation="MEGIIATPEFQVSLLLFAALGGYLLASRINQSAVVGIILVGLLI
                     GPSVLGLVSYNEFVRGLAHLGAVVLLFVIGFEFKLRDILKPSYALIALVGVIVPWVGG
                     YATAIWAGMDFASGLFIGTALTATSIAITANVLQELDKLQTGPARAIIGAAVIDDVLS
                     LVALAITGDVVAGAFSPAVIFFILLKVAIFLVVAGAVGIFIVSRFLVRLDQTSFVAKY
                     PEFIFIFAMMFAFLYAMIAELVGLSAIVGAFIAGISFERLSLRKSLDVKEGAEFLKII
                     FAAIFFVSLGVVADIKAITPEIVPFLVVLTVVAIVTKVVGCALPARAAGMCTKDSLII
                     GFGMAPRGEVAMIVALIGLEQEYIGQEVFVIIILMSLITTLITPIVYRNWFYKEEYCK
                     PETEI"
     gene            complement(5820..>6629)
                     /locus_tag="PKL26_00665"
     CDS             complement(5820..>6629)
                     /locus_tag="PKL26_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNS82238.1"
                     /translation="FMRLFPARCQWIAREERVARSGHLVIIGYGVNGKNLARAAKMGG
                     IEYAIVDMNPDSVRHATLEGEPIIYGDATTEGVLDHAGIDTARIAVVAINDPVATRRI
                     VGLCRQKNPGIFLITRTRYLVEVPVLREIGADEVIPEEFETSIEIFMRVLRAYGVTGD
                     SVEHLVRDIRADNYQALRSTSAPPVVAALDIPGMEVREVTVEDGSPIAGKTLGELLLK
                     KRYGVTVLAIRRNGALIPHPDGTCAMRAGDVAVLTGTIEKIEESLVLFLSP"
BASE COUNT         1380 a         1963 c         1838 g         1448 t
ORIGIN      
        1 ggcagcacat caggcctgat aaagatgatg accaacctcg gatcctccct tggtgtggcc
       61 ctcgtgatgc tggtcgctac cgctgctatg ggcccggctc ttgcgcattc ctctgcgcac
      121 acccttccgc ctgtagaact tgcaggtgca ttccacgtcg cgttcctctt tctcatgtgc
      181 atcgagatcc tggggatact cctgatgctc cctgtgaagg agaaagaaag cgggtactcc
      241 acagagggag agccggtcac tgccttttaa aaggaggaga tcaggcggga aggaccctca
      301 tctccatccc tgctatctgc ctgtatacct tttctttctc ggcgagcgtc cagcggtcga
      361 agaccaggga ctggaatggc tgccagatca ccacccagca gaatatcagc aggacattct
      421 ggaggatgat cgcgaggggt tcatggggaa agggatcaca tgccacaatc ccaagcagcg
      481 ccgggacaca caccgcaatg gtaagctgga actccctcag ccccactctc ccggaaagcc
      541 tcctctcccg gtgaatctcg tcggcgcgct taagaaaaaa tgcccgtatt gctgaagaga
      601 tgtcagtctc cctctgaaac gtgtaatcgg gtgcggggat gcggatatcc aggagataat
      661 cccccgctgc ctgcaggggt tttcgggtgt caaggactgc gctgcatatg acctcctcag
      721 cccgttctgt aagttccctg gaggccccgg gtgaaggatc gtcagagtcg taaatgtggg
      781 caatagtctt gaacataaca tgtagaggtt ctttatcatt agagtgcgac aggatctgca
      841 tggaaaatct cccgcaattc tcttttctgt agtgtgttga agatgatgag gatttttggc
      901 cagaaccagg tgcagcaccg gaatgcacgc gggagatgga tagccagtga aagcgtccct
      961 tagaagatgc aataagaggt gaaacgttaa gtaagagagc cgcacacctc acgcatgtga
     1021 cgggcggagg aattggatca tccgtatccg gggcgacaga agggggggat ggtcatgcat
     1081 acccagagat cgaaaagagg tatacgcatg ttcgtgcccg catccacagg ctctttgatg
     1141 agccgcctgg agatgacagg ctggcctttg gcatccaggc atttcttgca gcagtcgtcc
     1201 tcctcaatac ggttacagtg gtaatttaca ccgtcccctg gctggaggag gagtactggc
     1261 tccccctcaa tgggattatg accgcatgcc tgctggtctt cactatcgaa tatgtcgtgc
     1321 gcctatgggc atgcacgtcc gcacccaccc ttgcggcgag gacgacatat cgtatccagt
     1381 acgcatccag tttttacatg atcatcgatc tcgtatccat cgtgccgctg gtcttccccc
     1441 tcttcttccc aagggacttt gccctcttga gggcgttccg cctcctgtca atctttaaac
     1501 tcgggcgcta tgcacggaaa tcccaatctc ttgcccttct caagcgggtt gtcatgagga
     1561 aacgcgagat cttcacattg atggttttct tcctggtctt tgtcatcctt ttctcttcga
     1621 ccataatgta tctcgtggaa caccaggccc agcctgaaaa attctcgagc atccctgctg
     1681 ccatctggtg gtcgatgatg accgtgacca ctgtagggta cggtgatatc tacccggtca
     1741 ccccccttgg acagagcatc gcgtctctct tcaccctggt gggagtgctc ctcctcgcac
     1801 tcccttccgc gattctcgcg agtggtttca tcgaagaacg ccataaacag ggtgaatccg
     1861 gtgttgggat acccgtcgag caggaaatcg agctgctgga aaaagtcggc catctgcatg
     1921 agaaaggggt gatctccgag gaggaacttg aggattacag gcatactatc cagatactcg
     1981 cgggaaagag gggaagataa cctgctacag gagaggatct ctctgtcact caacctgttt
     2041 gagatcccct ggggataccc gtccaggcaa atccggaagg ctcactttct atacaccagc
     2101 caggaacata atagctgttt taatgagggc atttcctggc cagcttcagg cgagggcatt
     2161 catgaagaga agatggcagc gccggccttt gacgcggcag cggtcgactt acaggcaggc
     2221 tgaaaaactc catcgtctaa ttaggtaggg agagctggtg gtgattccat gatagaggtc
     2281 gatgtcttcc tgttcgacat tctcatcgtc cttggcctct ctatcctggt tctcttcctc
     2341 ggcaacaagt tccgggtgcc cggaattgtc gggttcatac tcgcggggat gctcgccggc
     2401 ccgtatggtc tcggccttat ccaggaccag gagattatcc agttcctggc cgagattggc
     2461 gtaatctttc tcctcttctc catcgggatg cagttctcgt tccggaccct ctaccacatg
     2521 aagaagatcg tcctgctggg gggagccctc caggtggggt tcaccattct tgtcactgcg
     2581 ggactggcat atatgctggg atttcccctg ccctccgcga tattcctcgg tttcctcata
     2641 tgccacacca gcaccaccat cactctacgg gtcttccagg accggggcga gctcgacagc
     2701 ccgcacagcc ggacgacgct tggcatctct ctcttccagg acctgatgag cgtcccgatg
     2761 atcctgctcc ttcccgctct ctcggggcat gatatccagc ctggggaatt tgcaatcacc
     2821 gttgaggtgg cgcttgtcgc atcacttgtc ctgatgctcg tgctcctctt ctaccttctt
     2881 ccccgcctga tggataccat caccaggacc aggagccagg aactctttct ccttaccatc
     2941 ctcttcgtct gccttgcaat cacctggatc acatcgagca tcgggttgtc acttgcactc
     3001 ggtgcgttcc ttgcggggct tctccttgcc gagacgccat attttcacca ggcgttcgcc
     3061 accatccttc ccttccgtga tatcttcacc agtttcttct tcatctcaat cgggatgctc
     3121 ctgaatgcgc acatactgat cacccacccg ctcattgtac ttggattgat cgcaggggca
     3181 attatcctca aggcagtgat cgccgggctc tcgtcactct cgctgggcca gtctctccgc
     3241 acctcggtgc tcgcagggat tgcactcgca aatattgggg agttctcgtt cgtcctcatc
     3301 cttgcaggaa tggagtttgc gttcctctcg gatttctcca cccaggtctt cctgaccgtg
     3361 gctgtctgga ctatggccct ctcaccgttc ctcatctccg ccggcccccg tatctccgga
     3421 tggatatgtg atctccccct tccccagaga atcaaggcag gttccgaacc tcctccgggc
     3481 acgttgcccg ccacccatcg cgatcacctg gtgatcgttg gtttcgggcc gaacgggagg
     3541 aaccttgccc gggtggcaga gatggagggg ataccctaca tcgtcatcga catgaacccc
     3601 gatacggtgg cggaagaacg aaagaaaggc ctacccatct tcttcggcga tgcggcaaac
     3661 ccctcaattc tgcaccattc cggaatagaa catgcgaggg tccttgcgat cgtcacgaat
     3721 gaccccttct ccagccggac catcaccgcg atggcccggg agatgaatcc cggaattcac
     3781 atcctgatca ggaccaggtt ccttcgcgat atcgaccagc ttcgggagat cggcgccgat
     3841 gaggtgatcg tggacgagtt cgagacctcg atcgagatca tctcaagggt gatgaaaagg
     3901 taccttgctc cccgtgtgac gatagaccgg gcaacctccc ggatacggga agacatgtac
     3961 agggtcttca ggcagccgcc ttctgacagg ggaatattct cggagatcgc gcttcgcatc
     4021 ccgcaggtag aggtgcagac tttcccgcaa cctggaaagt cgcccatggc gggccggact
     4081 cttcgcgaac aaaaagtccg gacggaattc ggggtctcgg tgattgcaat cgggaggaac
     4141 gaggagaccg tacccaaccc tgatgggggg ttcagggtgg atgccggaga tcgccttgtg
     4201 gtaattggaa agccggatca actggcgtca tttgccgcgg ttgttgagat ggatggcacg
     4261 ggcgcgtgac ccccctgcct ccgctgttcc tgccttaaaa aaatggctct tcgctatctg
     4321 atgggggtag gattgctgaa ccggacacat tttgtggggg ctgcccgccc ccaaccccca
     4381 tatgcgatac acgccgccgc ccccaaaggg gcgcccccgc ggcggttgga gtgactgaat
     4441 tgatacgcag acaacaacct tggttcagtc tccccaccca aaacagcgag gaggcaaaaa
     4501 aattggagca tttatatctc tgtctccggc ttgcagtatt cctccttgta gaaccagttc
     4561 cggtagacaa tgggagtgat cagcgtggtg ataaggctca tcaggatgat gatcacgaac
     4621 acctcctgcc cgatgtattc ctgttcgagc ccgataagtg ccacgatcat ggccacctcc
     4681 ccgcggggag ccatgccaaa accgatgatg agggagtcct tggtgcacat ccccgcagcc
     4741 cgggcgggca gggcgcagcc cacgaccttt gtgacgatgg caactactgt caggaccacg
     4801 aggaacggga ctatctcggg ggtgatggcc ttgatgtccg ccaccacccc gagcgagacg
     4861 aagaatatgg cggcaaagat gatcttcagg aactccgccc cctcttttac gtccaggctc
     4921 ttgcggagtg agagccgctc gaacgagata cccgcgataa atgcccccac gattgccgag
     4981 agccccacaa gctctgcgat catcgcgtag aggaaggcaa acatcatcgc gaagatgaag
     5041 atgaactccg ggtactttgc cacgaatgac gtctggtcca gcctgaccag gaacctgctg
     5101 acaatgaaga taccgacggc tcccgcgacc acgaggaata tcgcgacttt caggagaatg
     5161 aagaagatga ccgccgggga gaaggccccc gccaccacgt cgccggtgat ggcgagcgcg
     5221 accaggctga gcacatcgtc gatcaccgcg gccccgatga ttgccctggc cgggccggtc
     5281 tggagcttgt cgagctcctg gaggacattt gcagtaatgg caatgctcgt cgccgtgagc
     5341 gcggtcccga tgaaaagccc gctcgcgaag tccatcccgg cccagatggc cgtggcatag
     5401 cccccgaccc aggggactat cacccctacg agtgcgatca gggcatacga aggcttgagg
     5461 atgtcccgga gcttgaactc gaaaccgatg acaaaaagca ggaccaccgc accgaggtgg
     5521 gccaggcccc ggacaaattc gttatagctc accagcccga gcacgctcgg ccctatcagg
     5581 agcccgacaa ggatgatgcc caccaccgcg gactggttga tccgggatgc cagcaggtat
     5641 cccccgagtg cggcgaagag gaggaggctc acctggaact ccggggtggc gatgatgcct
     5701 tccatgccga tccattggga gttacgttca gaaaactctt ctgtgatcac gggggcggct
     5761 ggaatatagt ccccggatgt gagcgtgggg gtgctggcag ggtcccgcgt ggcttttact
     5821 cagggactca aaaagaggac gagggactcc tctatcttct caatggtccc ggtcaggact
     5881 gcgacatcgc ccgccctcat ggcacaggtg ccatcggggt gcgggatgag ggccccgttt
     5941 cgccttatcg cgagcaccgt gacaccgtac cgtttcttca ggagcaactc tccgagggtc
     6001 tttcctgcga ttggggaccc gtcctcaacc gttacctccc tcacctccat gccagggata
     6061 tcaagtgctg ccacaacagg cggggcggag gtgctgcgaa gggcctggta gttgtccgca
     6121 cggatatccc ggaccaggtg ttcgacagag tcccctgtaa ccccgtacgc cctcagcacc
     6181 cgcatgaaga tctcgatcga agtctcaaac tcttcaggaa tgacctcgtc ggcgcctatc
     6241 tcccggagga ctgggacctc gacaaggtag cgggtacggg tgatcaggaa tatgccgggg
     6301 ttcttctgtc ggcacagccc gactatgcgt cgtgttgcga ccggatcgtt gatagccacc
     6361 accgcaatgc gggccgtgtc tatcccggca tggtcgagca ccccctcggt cgtcgcatcg
     6421 ccgtagatga ttggttcccc ctccagtgtt gcatgccgga ctgagtcggg gttcatatcg
     6481 acgatagcat attcaatccc gcccatcttc gccgcccgtg cgaggttctt gccgttcacg
     6541 ccgtagccga taattacgag gtgcccggac cgggccaccc gttcctcccg cgcgatccac
     6601 tggcagcgag cgggaaagag cctcatgaa
//
LOCUS       DAOQVJ010000016         6548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_12947_length_6548_cov_4.282612, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000016 DAOQVJ010000000
VERSION     DAOQVJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12947_length_6548_cov_4.282612"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..939)
                     /locus_tag="PKL26_00670"
     CDS             complement(37..939)
                     /locus_tag="PKL26_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HNS82239.1"
                     /translation="MPETRSADAPGRPDRENRGDEHVIEAIGKCRIVIRNGRVTEVGQ
                     PAIRDCPLAKRFACPIPSIDQEAVKANIEHRIASFGMCTPDRVVLETREFVGFGASEI
                     LAFGLDSGLIDAVVLACDGAGTVVVTTPALVQGIGGRMSGLVSTTPYPEVIQRIEECG
                     GIVVDRHHARIDQAAGTGRAYKEGHRRVAATVALPEEAERIRTSYPDAFIFAVHTTGL
                     SREAAEVIVGSSDLVTACASGPIREIAGKKALLQAGIAIPVFAITARGKELVIEKIRQ
                     GREQVLVKTTRLPALGDQQPDPLV"
     gene            complement(952..1569)
                     /locus_tag="PKL26_00675"
     CDS             complement(952..1569)
                     /locus_tag="PKL26_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82240.1"
                     /translation="MRPVWYVLLAAVLAGILFSCGCTSQPTSSTQAGAATTSVISAVE
                     TSPDMLPVIALHLSDFPEGFELIYAGDTEPPAESTLLADPAYRGGYSVTVSNESSDFP
                     TGELVDQIVLIYSRPVTRERLAEVFTENYPELSTWPLTTLEDPGIGDASIAYHFTYPD
                     TTLSGYTIAFGRKDVYQILTTMTGDGTADYDFLKGIAEKALAKIP"
     gene            1927..6180
                     /locus_tag="PKL26_00680"
     CDS             1927..6180
                     /locus_tag="PKL26_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82241.1"
                     /translation="MITVLYVDDEPGLLEVSKLYLERHGEFHVETATSARDALELIKK
                     GNYDAVISDYQMPVMNGIDFLKEVRASGNPIPFIIFTGRGREEIVIQALNEGADFYLQ
                     KGGEPGSQFAELAHKVRQAVQQRRAEIRIRDHERREADILNFLPDATFAIDKSGVVIA
                     WNRAIERMTGVKAEHIIGKGNYEYAIPFYNERRPILIDLVRRDDPATAAKYPLIQREG
                     KNLFSEITIPHFNNGLGAALWFTASPLYNTHGEVVGAIESIREITERKKVVDALNESE
                     RRFRELAEMLPQVIYEADADGRVTYANRIAFEMFGYSEEDLRKGVNAMDMVMPDDRNR
                     AEVAFRNVLEKGSPEQQTREYRALRKDGSTFPVSIYSSPVVEDGRIVGVRGIIVDITD
                     RIRAEEKIRESEQNYRLLFETATEGILVAQGDRMVHVNPALIRLLGYPADILTSRPFT
                     DFIHPEDRGKVLNRHLQRMRGEIPHTGYTFRITRGDGEERWVWINSTRIQWSDSPASL
                     SFLTDITEQKKAEQQLISANREYAHLLDQIQDIYYRSDVQGRLTRASQSWARLLGYGD
                     LLECIGRNIADDFYINPSDRQPLLEAISLSGKVTNYEVMLRKKDGTPVCVETSSHLYY
                     DEEGNILGVEGTFRDITERKEQESILQAELNLGLALQKVRGLHDTLETCLKAAIDVSG
                     MDSGGIYLVDRGTGAVNLITSINLGDAFVKSVSHYPPTSDTARIIREGTPLFIPYGDL
                     EVSYHTPEAEKEGILSIAVIPITSGGKSISCLNVTSHKDIEIPGNRRVALATIATQIG
                     AAIERIMVEETLAESEQQYRNVVEDQTEFISRFLPDGTHVFVNDAYCRYFGFTRKEIL
                     GHRFKPVIPDEERERVQQFFESLTEENPVGSIEHRIIMPDGSIRWQRWSDRAIFDAGG
                     TLIEYQSVGRDITDIRDTLELLHEQEDWYSGLSQVSPDLIFMVDREDRVVYVNSAAAE
                     MLGKGPDEIIGHGRNSLFPPEVSEKQEYAISRVLETGERFRSEGQMHLGGSPRWFDHL
                     LYPVRDRQDNVTGIFGISRDITEQKKIEEDLRIRSEELDSRHRILTTLLETVPIGIFM
                     VEAPTGKPIIANREATRLLGRGVLPDATEGTLAEVYEAFKAGTCRKYPADEMPIIRGM
                     RGEVSHVDDMIVVRPDNTRAHLEVFGTPVIDRHGNVSASLVSFFDITERKQAEMSMKE
                     INKKIALLSSITRHDVANQVTLLRGLAQIAMEKETDGDISDILLKIDHAGATIARQIE
                     FTRTYQEIGMHEPAWFSLAAIIDKLQQWRVPLISTCSNAEVYADPMIENVFSNLFDNS
                     MRHGGHVTAISVRCEQNQDHLAIIYEDNGTGIPGEKKEKIFERGFGNHTGLGLFLSRE
                     ILGITGISLIETGVYGRGARFEMHVPTGAFRNLSCSLPG"
     gene            complement(6272..>6548)
                     /locus_tag="PKL26_00685"
     CDS             complement(6272..>6548)
                     /locus_tag="PKL26_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNS82242.1"
                     /translation="IGILISGHEDGAIKTALDISLVFEQMGYILAPFGIAFRTHGSQY
                     NSGTDAEFFRNDEMIKNDVRGVVSNVVELMLLDLESRLKGRLVPVSE"
BASE COUNT         1550 a         1737 c         1875 g         1386 t
ORIGIN      
        1 gagggaaaat gagaaccccc gcccctgtac ccgttatcag actagcgggt cgggttgctg
       61 gtcaccgagc gcagggagcc gcgtggtctt gacgaggacc tgttcgcgtc cctgccggat
      121 cttctcgatg acgagctcct ttcctctcgc ggtgatggca aataccggga tggcaatccc
      181 ggcctgcagg agtgcttttt tcccggcaat ctcccggatc ggcccggatg cacatgccgt
      241 gacaaggtcc gatgagccga cgatcacttc cgccgcctct ctcgaaaggc cggtggtgtg
      301 gaccgcaaat atgaacgcat ccgggtagga cgtgcgtatc ctctctgcct cttccggcaa
      361 ggccaccgtc gctgcaactc tgcgatgacc ctccttatac gcacgcccgg tgccggctgc
      421 ctggtcgatc ctggcatggt gcctgtccac gacgataccc ccgcactcct caatcctttg
      481 tatgacctcg ggatatgggg tggtcgagac gagccccgac atcctcccgc caatcccctg
      541 gacgagagcg ggggtggtca cgaccacggt ccccgccccg tcgcaggcaa ggacgaccgc
      601 gtcgatgagg cctgaatcga gtccgaacgc gaggatctcg gatgcgccga aaccgacgaa
      661 ctcccttgtc tccagcacga cccgatccgg ggtgcacatg ccgaaggagg cgatccggtg
      721 ctcgatgttc gccttcaccg cctcctggtc gatcgagggg atggggcagg caaaccgctt
      781 cgcgaggggg cagtccctga tcgcgggctg gccgacttcc gtgacacgcc cgttccgtat
      841 cacgatccgg cattttccaa tcgcctcgat gacatgctcg tctccccggt tctccctgtc
      901 cggccttccg ggtgcgtctg cgcttctggt ttcaggcatg accgttacat ttcaggggat
      961 ctttgcgagg gccttttcgg cgatcccttt caggaaatcg tagtccgcag tgccgtcccc
     1021 tgtcatcgtg gtgaggatct ggtagacgtc tttcctgcca aaggcgatgg tataccctga
     1081 gagcgtggtg tcagggtacg tgaagtgata cgcaatgctg gcatccccta tcccgggatc
     1141 ttcaagcgtc gtgaggggcc aggtggagag ctcggggtag ttctccgtaa agacttcggc
     1201 gaggcgctcc cgggtcacgg gccgggagta aatgaggaca atctgatcga caagttctcc
     1261 cgtgggaaaa tcagaactct cgttgctcac cgtcaccgag tagcccccac ggtaggctgg
     1321 gtcggcaagg agcgtgctct ctgccggggg ttcggtatct cccgcataaa tcagctcgaa
     1381 gccttcgggg aagtctgaca ggtgcagggc aatgacagga agcatgtcag gggaggtctc
     1441 cactgcagag attacactcg tggttgccgc ccccgcctgg gttgatgatg tcggctgcga
     1501 ggtgcacccg caggagaaga ggatgcctgc cagcaccgct gccaggagta cgtaccacac
     1561 aggtctcatt cagaaccact ttgatcacct tgaaatctcc cacccaatag tggttttggt
     1621 tcattcctgc acaatgtatt ggcaggagag cagggtttgc tgtcccgggg tcgagaagga
     1681 tctgactggg aataaagaca ggtgggaaat aacggtgaat tgaaagcaat caagaatcag
     1741 gatagtttca aagtgcaaca ttgaatgaaa tgggagtgca ggcgcgagat attgagccgg
     1801 ggaacgggat gattctccct gtaaggtgta taaattgcac ttcaccagag tatccctttc
     1861 cagaagaatt accaaaagag taactatcaa tacactccgg aataatgatg caatgtacca
     1921 acaaccatga taaccgtcct ctatgttgat gacgaaccag ggcttctcga ggtaagtaag
     1981 ctgtacctgg aaaggcatgg cgaatttcac gttgagaccg caacatctgc gagagatgct
     2041 cttgagctca tcaaaaaagg aaattacgat gcagtcatct ctgattacca gatgccggtg
     2101 atgaacggga tagatttctt aaaagaggtc cgtgcttcgg gtaatccaat tcccttcatc
     2161 atctttaccg ggcgtggtcg tgaagagatc gtcatccagg cgttgaacga gggtgctgac
     2221 ttttatctcc agaagggtgg ggaacccggg tctcagtttg cggagcttgc acacaaggtc
     2281 cggcaggcag tccagcagag gcgggcggag atacgcatcc gggaccatga gcgacgggag
     2341 gcagatatcc tcaatttcct gcctgacgcg acgtttgcca tcgacaagag cggagtggtg
     2401 attgcatgga accgggctat agagaggatg accggggtga aggccgaaca catcatcggg
     2461 aaaggcaact atgaatatgc cattcctttt tacaacgaga gacgaccgat tctcatcgac
     2521 ctcgtgcgaa gagacgaccc ggctaccgct gccaaatatc cactcataca gcgcgaagga
     2581 aagaacctgt tctccgagat tacgatcccc catttcaaca acggcctcgg tgcggccctc
     2641 tggtttaccg catcgcctct ctataatacc cacggggagg tcgtcggcgc catcgagtcg
     2701 atccgcgaaa tcaccgagag aaaaaaagtg gtggatgctc tgaacgagag cgaaaggcgc
     2761 tttcgtgaac ttgcggagat gttacctcag gtaatctacg aagccgatgc cgatggcaga
     2821 gtgacgtatg caaaccggat tgcgttcgag atgttcgggt attccgagga ggatctccgg
     2881 aagggagtga atgccatgga catggtcatg cccgatgatc ggaatcgggc tgaagtcgcg
     2941 tttcgcaatg tgttggaaaa aggatctcct gaacagcaaa cgagggagta cagggcactc
     3001 cggaaagacg gcagtacgtt tcctgtctct atctattcct ctcccgtggt agaggatggc
     3061 aggattgtcg gcgtgagggg aatcattgtc gatatcaccg atcgcataag ggctgaggag
     3121 aagattcgcg agagtgaaca gaattaccgg ctcctctttg agactgcgac tgaaggcatc
     3181 cttgtggcgc agggtgaccg catggtgcat gtcaatcctg cgctgatcag gcttcttggc
     3241 taccctgcag atatcctcac ttcgaggcca ttcacagatt tcattcatcc tgaagatcgc
     3301 gggaaggtat tgaaccgcca tctccagcgg atgaggggag agatacccca cacaggatac
     3361 acattcagga taaccagggg agacggtgag gagagatggg tctggatcaa ctcgacacgg
     3421 attcagtggt cagacagccc tgcgtcgctc tctttcctca ccgatattac cgagcagaag
     3481 aaggccgaac agcaactgat ctccgccaac cgggaatatg cacacctgct cgaccagatc
     3541 caggacatat attacaggag tgacgtccag ggccggctta caagggccag ccagtcgtgg
     3601 gcaaggctcc ttggctatgg cgatctcctg gaatgcattg gacggaatat cgcggatgat
     3661 ttttatatca atccttcgga ccggcagcct ctcctcgagg cgatctccct gagtggcaaa
     3721 gtgacaaact acgaggtaat gctcaggaag aaggatggca cccccgtgtg tgtcgagaca
     3781 agcagccacc tctattacga tgaagaaggc aacatcctcg gggtcgaggg aacatttcgt
     3841 gatatcacag agcggaagga acaggaatcc atcctgcagg ccgagctcaa tctcggcctt
     3901 gcactccaga aggtgcgggg attgcacgat acgctggaga cctgtcttaa ggcggcaata
     3961 gatgtatccg ggatggattc aggaggaatc tatctcgttg acaggggaac gggggcagtg
     4021 aaccttatta cttcaatcaa cctaggagac gcgttcgtga agagtgtctc tcattacccc
     4081 ccgacgtctg acactgcacg gattatcagg gagggcactc ccctcttcat cccctacgga
     4141 gatctggagg tttcatatca cacacccgag gcagagaagg agggaatcct gtccattgca
     4201 gtaatcccga tcacttcggg tggaaaaagt atctcctgtc tgaatgtcac ctcgcataag
     4261 gatattgaga tccctgggaa caggcgtgtt gccctcgcga cgattgcgac gcagataggg
     4321 gcagcaattg agcggatcat ggttgaagag accctcgcgg agagcgagca gcagtatcgg
     4381 aacgtggtcg aagaccagac cgagtttatc tcccggtttc taccggacgg cacgcacgtc
     4441 ttcgtaaatg atgcctattg caggtatttc gggttcacgc gcaaagagat cctcgggcac
     4501 aggttcaagc ccgtgattcc tgacgaggaa agggagcgtg tacagcagtt cttcgaatcc
     4561 ctgacagagg aaaatcccgt gggaagtatt gaacaccgga tcatcatgcc ggatggcagc
     4621 atacggtggc agagatggag tgaccgtgcg atcttcgacg caggaggaac actcattgaa
     4681 taccagtcag tgggcaggga tatcactgat atcagggaca ccctggagtt attgcacgaa
     4741 caggaggact ggtattcagg cctatcgcag gtatcccctg atctcatctt catggtcgac
     4801 cgcgaagacc gggtcgtata tgtaaattcc gccgctgccg aaatgctcgg gaaaggacct
     4861 gatgagatca tcggccatgg gagaaacagc ctgtttcccc cggaagtctc tgaaaagcag
     4921 gaatatgcca tttcacgtgt gctcgagaca ggggagcggt tcaggagtga agggcagatg
     4981 catctcggag ggtccccgcg ctggttcgac cacctccttt accctgtaag ggacaggcag
     5041 gacaatgtca cggggatatt tggcatttca agggacatca ccgaacagaa gaagatagag
     5101 gaggatttac gcatcaggag cgaggagctc gacagccgtc accggatcct cacgacactc
     5161 cttgagactg tcccgatcgg catcttcatg gttgaagcgc cgacgggtaa acctattatc
     5221 gccaaccgcg aggcaacccg tctccttggc aggggtgtcc ttccggatgc gaccgaggga
     5281 accctcgcgg aagtatacga ggcctttaag gcaggcacgt gtagaaaata ccccgccgac
     5341 gagatgccca tcatacgggg gatgagaggg gaggtgagcc atgtcgacga catgatagtc
     5401 gtgcgccccg ataatacccg tgcgcatctg gaagtgtttg gcactcctgt cattgaccgg
     5461 cacggcaatg tctctgcaag ccttgtcagt ttctttgata tcaccgagcg caagcaggcg
     5521 gagatgtcga tgaaggagat caacaagaag atcgcacttc tttcaagcat tacacggcat
     5581 gatgttgcaa accaggtgac cctcctccgg gggcttgcac agatcgcgat ggagaaagaa
     5641 accgatgggg atatctctga tatccttctg aaaattgacc acgcaggggc cacgattgca
     5701 agacaaatcg agtttacccg gacctaccag gagatcggga tgcacgaacc cgcatggttt
     5761 tcccttgcgg caataattga taaactgcag caatggcggg tccctctcat cagcacgtgt
     5821 tcaaacgccg aagtctatgc agatcccatg attgaaaatg tcttctcaaa tctcttcgat
     5881 aactccatgc ggcatggggg tcatgtgacg gctatctcgg taaggtgcga acagaatcag
     5941 gatcaccttg ccatcatcta cgaggacaac gggacaggca tccccggaga gaagaaggaa
     6001 aaaatattcg agcgcgggtt tgggaaccac accggacttg ggctcttcct gtcacgggag
     6061 atcctcggga ttaccgggat ttctctcatt gagacgggtg tatatggcag aggtgcacgg
     6121 ttcgagatgc atgtgccgac aggagcgttt cggaatctct cctgttccct gcccggctga
     6181 cgggaatacc agggagatac ctcttggata attaaaaaat ctgattactc cttcaaatcc
     6241 tctcgaattc tgtccacatt ctctctctgg atcactccga caccggcacc agcctccctt
     6301 tcaggcgcga ctccaggtca agcagcatca gctcgaccac gttcgagacg actccccgta
     6361 catcgttctt tatcatttcg tcgtttctga agaattccgc atcagtgccg ctgttatact
     6421 gcgatccatg cgtcctgaac gcgatcccga aaggtgccag gatatacccc atctgctcga
     6481 atacgaggga gatgtcaagc gcggtcttga tcgccccgtc ctcgtggccg gagatgagga
     6541 tcccgatg
//
LOCUS       DAOQVJ010000017         6515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13029_length_6515_cov_5.656811, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000017 DAOQVJ010000000
VERSION     DAOQVJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13029_length_6515_cov_5.656811"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PKL26_00690"
     CDS             complement(<1..991)
                     /locus_tag="PKL26_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82243.1"
                     /translation="METETYDKDKVVFAATMASTFEPDSMTNDKIEAATRGHGALVIP
                     VFAAANSIAEDLFCPITGPEMSLMGRMTVVDASVGELPLDVVIQKAVRAAMSAGAAPE
                     NAALIVASLAYFSGSCARSGVPLGNRKLGAIARMHAGAARTSAIALVTGKSTHRIQSF
                     PAYLAIYEAMMDKKLTKVDGSMLPPFISGGAIYGHSALGEDYNIPELAYNAAHAGTLA
                     MMKSMAGTGITPLALWPALIGAAVTMEIVHPDALLGEEFGKFGRVDSSHLAGKGARDA
                     AKLPEKIHIRGTHEEYDTAKIIGEFGLILKDIGGPSVIGSMALSEIFAAFEEAP"
     gene            complement(1222..4728)
                     /locus_tag="PKL26_00695"
     CDS             complement(1222..4728)
                     /locus_tag="PKL26_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82244.1"
                     /translation="MISVLFVDNEPHFCELVKSTLEQSGLFSVDTAESASAALGKMKH
                     GSYDAIVSDYQMPDMNGIEFLKALRASGNEIPFIIFTGKGREEVVIQAYENGADFYVQ
                     KSGDPEVQYADLAQKITRAVGLHRAEHDRLESEDRLRQIIQFLPDATFAIDMEGRVIA
                     WNKAIEEMTGAPASQMIGKGKYEYAIPFYGERRPILIDLAMQPDEETTSRYAFVRKEG
                     RFLEAEASNARLQGKDVVLWGKATPLYDTRCRLTGAIESIRDITGFKKTEETLRESEA
                     RYRRFAENAKDLIYRYTFTPIRGFTYVSPSSTNIVGYTPEEHYADPDIGWKLVFEDDR
                     HLLESLAHDPGVYKKPLVVRWKKKDGTVIWTEQINHPIYDNEGNIIAVEGIARDITDR
                     TRSEEAIREKQIQLANAMDLSHLVMWEYDVASDMFTFNDNFYSLYGTTASQEGGYQMS
                     SKEYAARFVHPDDVHFVAEEVQNALKSTDPSFSSQLEHRIVRRDGEVRFIIVRIAGVA
                     GESGTLIKTYGANQDITDRIKAETTLRESEARYRTFVENAAEGVVVIQDGKIVYANPA
                     ALLLTRESLENLLEKPFGQYIHPDDRDMVVGRYKRRMLGEDLPSIYDFRLAGTDGYET
                     WVQISVAVIPWNGRPATLNFLSDITERKQREQLLQIQHRIATLCSSPAPLSEILPECM
                     HHVLALTRMDAGGIFLVDEHSGLSLVYSTGLSEAFVREEQYHMQDSPQTEFVMQGRPY
                     FGDFSSLQFPPSPARASEGIRALAVVPFVHQGRVTGCMNLASHTRETFPQVIQEAIST
                     LAIQMGSDISRIQASEALRKSEEKFRRMVETAAEGIWEMDQDFHITYANQRMADLLGY
                     TREEMVGREISTFMDRDELPDSLARKERQRQGIADHFERKLIRKDGMPLWIYSSVTPV
                     FDADGRFRGSFAMYSDITDRKLIEAEIRSLNQVLEQRVGERTAELTRLLHEREVLIRE
                     VHHRVKNNLQIIISLLNLQGKYITDQKVLAAIRESQNRIKSMALVHERMYQSEEVSRI
                     TFREYVRFLVSHLFTFYDVRAHQVSYTITMDPFPIDIDTAIPLGLIMTELVSNSLKHA
                     FPAGRKGTISIEGRKVDGHTFRFVVKDNGVGISPEIDWKNSDTLGLRLVKSLVTQLNG
                     TIELDRADGTTFTLHVRAKEAK"
     gene            complement(5104..>6515)
                     /locus_tag="PKL26_00700"
     CDS             complement(5104..>6515)
                     /locus_tag="PKL26_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS82245.1"
                     /translation="DIAIVDAVARLSPGETLTSERRAEIPFDPKTRWNAVLASLPAGP
                     VLVVRGAPEEILRLSPSLPEAGRMAVADWLSRNGLAGERTIAIAKKALPPGTTGISPG
                     DVQDLEFLGATAFTDPIKPSAFRAVEMARELGVALKIITGDSPEVAGAVGVRIGLLED
                     PAAVVTGSALAAIPPGEREQVIQKHAVFARITPEQKFEIIETLQRTCTVGFLGEGIND
                     APALKRAGVSLVVDSAADIAREAADIILLQKDLEVIIEGIRSGREVFANSVKYLKATL
                     ASNFGNFYAIAIVSLFIPFLPMLPIQILLVNLLSDFPMISIATDTVDEAEFRLPGRYD
                     LKEIVLIATLLGVISTFDDFAFFAVFVGDGAQVLQTSWFIGSILTELVFLFSIRTRLP
                     FYRARRPSIHVLALTTVAAAATILLPYTSIGQALFSFVPPSPGAIAVILGLVAAFFVT
                     VELVKVNYYRYAGGLPARS"
BASE COUNT         1334 a         1854 c         1814 g         1513 t
ORIGIN      
        1 tcggggcttc ttcaaaggcc gcgaaaatct ctgaaagggc catcgatccg atcaccgaag
       61 ggccgccgat atccttgagg atcagcccga actcgccgat gattttggcc gtgtcgtact
      121 cctcgtgagt tccccggatg tgaatctttt ctggcagttt cgcggcatcg cgtgctcctt
      181 ttcccgcgag atgcgatgaa tcgaccctgc caaacttccc aaactcctcg cccaggagtg
      241 catcggggtg cactatctcc atcgtgaccg ccgcgccgat aagggcaggc cagagcgcga
      301 gtggggttat cccggtgccc gccatgctct tcatcatggc gagcgtgcct gcatgtgcgg
      361 cattgtacgc caattcaggg atgttgtaat cctcaccaag tgcggagtgg ccatagattg
      421 ccccgccgga gatgaacggg gggagcatgg agccgtccac tttcgtgagc ttcttgtcca
      481 tcatggcttc gtagatcgcg aggtatgccg ggaacgactg gattctgtgg gtggacttgc
      541 cggtgaccag cgcaatcgca cttgtccggg ctgcgccggc atgcatccgg gcaatggcgc
      601 cgagtttcct gttgccgaga ggaacacccg accgtgcaca ggaacccgaa aaatacgcga
      661 gtgacgcgac aatgagcgca gcgttctcgg gagcggcccc ggcactcatc gcagcccgca
      721 ccgccttctg gatgaccaca tcgaggggga gctccccgac tgatgcatcc acaacagtca
      781 tccgccccat caggctcatc tccgggccag tgatagggca gaagagatcc tcggcgatgg
      841 aattggcagc tgcaaagacc gggatgacga gggcgccatg tcccctggtc gcggcttcga
      901 tcttgtcgtt tgtcattgag tcgggctcaa aggttgaggc catcgtggct gcaaagacca
      961 ccttgtcctt gtcatacgtt tctgtttcca tctgcatcac cctgtcaata gtgcacagtt
     1021 cacaatcaag cgtattaata ggttgggcga caggagatga gcgcgcagtt gtcccggtcg
     1081 gaaaaggcct gcggacctgc gttgcaggag tggttacaga gaatgaggca gttttggcca
     1141 tccccgcacg cggccttatc ctttcgcaac tctcttgaaa acccaaggag cgggaagcac
     1201 gcgtgataca tcatcctgga atcacttcgc ctctttcgca cgcacatgca gcgtgaatgt
     1261 cgtcccgtcc gcccggtcca attcgatggt cccgttcagc tgggtaacga gggatttcac
     1321 caggcgaagg ccgagtgtat ccgaattctt ccaatcgatc tctggagaga tccccacgcc
     1381 gttatccttc acgacgaacc tgaaagtgtg accatccacc tttctcccct cgattgagat
     1441 cgtccccttc ctgcccgcgg ggaaggcatg tttgaggctg ttcgagacaa gttctgtcat
     1501 aatcagcccg agggggatgg cggtatcgat atcgataggg aacggatcca tggtgatggt
     1561 ataggagacc tggtgggccc tgacatcgta gaatgtgaag agatgggaga ccaggaacct
     1621 gacatactct ctgaaggtga tccgggagac ctcctcggac tggtacatcc gctcgtgaac
     1681 aagggccatc gacttgatcc ggttctggct ctcccggatg gcggcaagga ctttctggtc
     1741 cgtgatatat tttccctgga ggttcaggag agagatgatg atctggaggt tgttttttac
     1801 ccggtgatgg acctcgcgga tcaggacttc ccgttcgtgc aataacctgg tcaactcggc
     1861 ggtccgctcc ccgacccttt gttcgagtac ctggttcagg gaccggatct cggcctcaat
     1921 cagtttccga tccgtgatgt cggagtacat tgcaaaagag ccgcggaatc ttccatcagc
     1981 gtcgaatacc ggagttacag aggaatatat ccagagtggc attccgtcct tccggatcaa
     2041 ctttcgctcg aagtggtcgg cgattccctg ccgctgccgc tccttccggg caagagagtc
     2101 ggggagttca tccctgtcca taaacgttga gatctctctt cccaccatct cctcgcgtgt
     2161 atagccgagc aagtcggcca tcctctggtt cgcgtaggtg atgtgaaagt cctggtccat
     2221 ctcccagatc ccttctgctg cggtctcgac catccgcctg aacttctcct cgctcttcct
     2281 gagcgcttca ctcgcctgga ttcgggagat atcgcttccc atctggatcg cgagagtcga
     2341 gatggcctcc tgtatcacct gggggaaggt ctcccgtgtg tgggacgcaa ggttcatgca
     2401 gcctgtcacc ctgccctggt gcacgaatgg cactactgcc agtgccctga ttccttcgct
     2461 cgcacgtgcc ggcgaaggtg gaaactggag cgatgagaaa tccccaaaat agggtcttcc
     2521 ctgcatcaca aactcggttt gcggcgagtc ctgcatgtga tactgctctt cgcgcacaaa
     2581 tgcctccgag agtcctgtcg aatatacgag gctgagtccg ctatgctcgt caacgaggaa
     2641 gatccctccg gcgtccatcc gggtgagagc gaggacatgg tgcatgcatt caggcaggat
     2701 ttcactgagg ggcgcgggcg atgaacagag agttgcaatg cggtgctgga tttggaggag
     2761 ctgctccctc tgttttcgct cggtgatgtc ggagaggaag ttgagggttg ccggccggcc
     2821 gttccagggg atcaccgcga ctgatatctg cacccacgtc tcgtacccat cggtccctgc
     2881 cagccggaaa tcgtatatcg aggggagatc ttcgcccagc atgcgccgtt tgtatctccc
     2941 gaccaccatg tccctgtcgt cgggatgtat gtattggccg aatggtttct ccagcaggtt
     3001 ctcaagggat tcacgggtga gaaggagcgc tgcggggttc gcgtacacga tctttccgtc
     3061 ctggatgacc accactcctt cggcagcgtt ctcgacaaac gtgcgatatc gcgcttcgct
     3121 ctcccgcaac gtggtctctg ctttaatgcg gtcagtgatg tcctggttcg caccgtaggt
     3181 ctttataagg gttcctgatt ctcctgcgac tcctgcaata cggacgatga taaaccggac
     3241 ctccccgtct cttcggacaa tcctgtgttc aagctgcgag ctgaaagaag ggtccgtcga
     3301 cttcaacgcg ttctggacct cttctgcaac gaaatgtaca tcatccgggt ggacgaaccg
     3361 cgccgcatac tccttcgacg acatctggta tcccccttcc tgtgatgcgg tcgtcccata
     3421 cagcgagtaa aaattgtcgt tgaacgtgaa catgtccgat gccacgtcgt attcccacat
     3481 gacaagatgc gaaaggtcca tggcattggc gagctggatc tgcttctccc ggatggcctc
     3541 ttcgctgcgc gttcgatcgg tgatatcgcg ggctatcccc tcgacggcga taatgtttcc
     3601 ttcattatcg tagatggggt gattgatctg ttctgtccag ataacggtgc cgtctttctt
     3661 cttccacctt acaacgagcg gttttttgta tacacccggg tcatgtgcaa gtgactcaag
     3721 gagatggcga tcatcttcaa atacaagttt ccagccaatg tccgggtcag cataatgctc
     3781 ttcgggggtg tatcccacga tattcgtgga ggagggggag acataggtga aaccccgtat
     3841 gggggtgaat gtgtagcggt aaatcaaatc ctttgcgttc tctgcaaacc ggcggtatcg
     3901 tgcttcactt tcacggaggg tctcttctgt ctttttaaac ccggtgatat ccctgatgga
     3961 ctcgatcgcg ccagtcagcc ggcaccgggt gtcatacagg ggtgtggctt tcccccataa
     4021 tacgacatcc ttcccctgca atcttgcatt cgaagcctct gcctccagga acctcccttc
     4081 tttgcggaca aatgcgtacc tcgatgtggt ctcttcatcc ggctgcatcg caaggtcaat
     4141 aaggatcggg cggcgctctc cgtagaatgg gatggcatat tcatatttcc cctttcctat
     4201 catctgcgac gccggagctc ctgtcatctc ctcgattgcc ttgttccagg caatcactct
     4261 tccctccata tcgatggcaa acgttgcatc gggtaagaac tggatgatct gtcgcagcct
     4321 gtcctcgctt tcaagccggt catgctcagc ccggtgcagt ccgactgccc tggtaatctt
     4381 ctgtgctaaa tctgcatact gaacctcagg gtctccgctt ttctggacat agaaatcagc
     4441 cccgttctca tatgcctgga tcaccacctc ttccctcccc tttccggtga aaatgatgaa
     4501 cgggatttcg tttccggatg cacggagtgc tttcaaaaat tcgattccat tcatatccgg
     4561 catctggtag tccgatacta tcgcatcata ggaaccatgt ttcatttttc ccaatgcggc
     4621 ggatgctgat tcagcagtat cgaccgaaaa taagcctgat tgttcaaggg tgctttttac
     4681 caattcacag aagtggggtt cattatcgac aaaaagaact gatatcaaag gaaatcgccc
     4741 ctaaaatagt ttttattcaa aaaaggatgc ctgcccattt tctaaaatcc acctggtgaa
     4801 ttaatagata cctatttgcg tctttttttt acaccccttt ataatttacg atattattta
     4861 tttgtcaaaa aaatcctgaa cattgggctc gcctttattg acctcaccag aatatgcgac
     4921 gcatatcaca tctctgcaca gggggaatta tgaaaaaaca tcaggttata ctatcccctg
     4981 ttcaggcagc cggaaatttg cctgcctgga cccttcctgt cggatgggca ggaggaggaa
     5041 atctcccctt tctccctccc aactctcctc tcccaaggag aaacacggta ttttcgtcca
     5101 ccatcaggag cgtgcgggga gtccgcctgc gtagcggtaa taattgacct tcaccagttc
     5161 tacggtcaca aagaatgctg ccaccaggcc gaggatgacc gcgatagccc cgggcgaagg
     5221 cggcacaaag gagaagagtg cctgccctat tgatgtgtag gggagcagaa tggttgcggc
     5281 cgcggcgaca gtcgtcaggg ccaggacatg gatcgagggg cgtctcgccc ggtagaaggg
     5341 aagtcttgtc ctgatggaga agaggaagac aagttcggtc aggatgctcc cgatgaacca
     5401 gctggtctgg agcacctgtg ccccgtcccc gacgaagacc gcgaagaacg caaagtcatc
     5461 gaatgtgctg atcacgccaa ggagggttgc aatcagcacg atctctttca ggtcgtacct
     5521 ccctggcagg cggaactccg cctcatcgac ggtgtcagtg gcgatcgaga tcatgggaaa
     5581 atcggagagg aggttcacga gcaggatctg gatggggagc atcgggagga acgggatgaa
     5641 gagcgagaca atggcaatgg cgtagaagtt gccgaagttg gatgcaagcg tcgccttcag
     5701 gtacttgacg ctgtttgcaa acacttccct cccgctccga attccctcga tgatcacttc
     5761 aagatccttc tggaggagga tgatgtcggc tgcctcgcgg gcgatgtcgg cagcgctgtc
     5821 aacgacgaga gagaccccgg cacgcttgag cgccggcgca tcattgatcc cctccccgag
     5881 gaacccgaca gtgcaggtcc gctgcagggt ctcgatgatc tcaaacttct gctctggtgt
     5941 gatgcgtgca aacaccgcgt gcttctgtat cacctgttcc cgctccccgg ggggaatcgc
     6001 cgcaagggcc gatcctgtaa cgaccgccgc cgggtcctcg aggaggccga tccgcacgcc
     6061 gaccgcccct gcgacttcgg ggctgtcccc cgtgatgatc ttgagggcca cgccgagttc
     6121 gcgggccatc tccaccgcac gaaatgccga gggcttgatc gggtcggtga acgccgtggc
     6181 gccgaggaac tcgaggtcct ggacatcccc gggggaaatt cccgtcgtcc cgggtggtag
     6241 ggccttcttc gctatcgcga tcgtccgttc cccggccagc ccgttccggg agagccagtc
     6301 ggcaactgcc atgcgcccgg cttcagggag agagggggac aggcggagga tctcttcagg
     6361 tgccccgcgg actacgagca cgggacctgc gggcaggctc gccagcaccg cgttccaccg
     6421 cgtctttggg tcgaagggga tctcggcacg ccgctccgat gtgagggtct ccccgggaga
     6481 gagccgtgcg accgcatcga caatggcgat atcga
//
LOCUS       DAOQVJ010000018         6472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13154_length_6472_cov_5.034440, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000018 DAOQVJ010000000
VERSION     DAOQVJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13154_length_6472_cov_5.034440"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1145
                     /locus_tag="PKL26_00705"
     CDS             3..1145
                     /locus_tag="PKL26_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82246.1"
                     /translation="MRYRYPRGMEAIRGISFHVRRGEKIALVGPNGAGKSTILLMFNG
                     MIRPDSGTILFDNVPISYDRASLRTLRKSVGFVLQNPDRQIISPTVYQDVAFGPTNLG
                     FTDEEVKEAVESALGRVGLSGFGRRPPHHLSGGEKKRVAIAGVLAMDPDVLVFDEPTS
                     GLDPSGSEDIMELLDELNQQGKTVIISTHDIELAYPWADRAILLLNGRILQEDVPDVA
                     FGNPELVRKARLSMPTVLELYLELGRRGFCLPERRPKTVLDMLNVIDRAFRNKPCYTQ
                     PGSITLFDIDSSHDTDIPGWCAAHPGVPVGAMGTRAKQRAADEKIWLDFTYGVIDKCI
                     LKALLGEHSLIIVTGSMVGRVHERVREFCRENQVEIQVSSLSKRHG"
     gene            1286..1663
                     /locus_tag="PKL26_00710"
     CDS             1286..1663
                     /locus_tag="PKL26_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82247.1"
                     /translation="MTRWSDTAAIPSRADSETLSVAFTLVFRQGRAPPSCPSPREAEL
                     LNQICDRVQAASPAACRDALIRVRKLSYDVYIVCDEFREGIFGTGNEAQAAAINALAE
                     INPGFSAEEYRTAFVTGMMWTAL"
     gene            complement(1668..2285)
                     /locus_tag="PKL26_00715"
     CDS             complement(1668..2285)
                     /locus_tag="PKL26_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS82248.1"
                     /translation="MPVSVLGLSGSPHRHGNTETLLDAFLEGADSAGGQVEKIILKDL
                     DYHPCRGCNACHKTGECVVKDDAIPLFNRILAAECLAVASPIYSMGITAELKGLIDRA
                     QFMWARKFILKNLYFPNEQIRRHKGIFISTAGLSWDNVFDAAFPAVTAFFNTLGFEYY
                     DNIIANDMDRYQGIRGHPTAIAEARKKGTEVVLLLEKLKEEEEPA"
     gene            complement(2289..2903)
                     /locus_tag="PKL26_00720"
     CDS             complement(2289..2903)
                     /locus_tag="PKL26_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS82249.1"
                     /translation="MEIKVLAFAGSPRRGGNSETLLDWVLASMGAEEGVVIEKIPLTE
                     ANVNPCRGCNACERLNRCVQRDGMDLLHDKIIEADCIILAAPIFCMGICAQAKALIDR
                     FQVFRSRKYVLKLPVVPPERKGKRLGIFLSTAGQDWDHVFDGAVPSVKCFFHVIDVRD
                     ADIHYLMVNNVDEKGAILQHPTAQADATRIGKEVITEVKKRLGG"
     gene            complement(2983..3624)
                     /locus_tag="PKL26_00725"
     CDS             complement(2983..3624)
                     /locus_tag="PKL26_00725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNS82250.1"
                     /translation="MCGEFSSMPILGEPAPDFEALTTQGMVKLSDFKGKWLVLFSHPA
                     DFTPVCTTEFIAFSAIAEDLKSLNVQLMGLSIDSVHSHLAWIRNVKEKMGVDIPFPII
                     ADLDMKVAKKYGMIHPGQSSTAAIRAVFFIDDKGILRAMIYYPLTAGRFMPEIVRLVK
                     SLQTTDKYGVATPANWQPGDKVVVPAPKNKEEMDRRLSEGYECKDWYLCFKNI"
     gene            complement(3631..3984)
                     /locus_tag="PKL26_00730"
     CDS             complement(3631..3984)
                     /locus_tag="PKL26_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS82251.1"
                     /translation="MAKETKNIKDLEKMIGKVPKFFKELAEKDPMMYEMVMKLEGHIW
                     DDGALTRKTKKLIAIAIAASLRDQHATRAQLAGAANLGIKKNEVEEALRVAFLLAGMP
                     AYVYGKAQLDELMKE"
     gene            complement(3992..4117)
                     /locus_tag="PKL26_00735"
     CDS             complement(3992..4117)
                     /locus_tag="PKL26_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HNS82252.1"
                     /translation="MVNVSKEGQQFECEICGNVVVVKVVGGGELVCCGQPMKLKK"
     gene            complement(4228..4461)
                     /locus_tag="PKL26_00740"
     CDS             complement(4228..4461)
                     /locus_tag="PKL26_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82253.1"
                     /translation="MIDLTEKAKAGQKVGPGKYVCLDCGRELTLDAVEQDLRKCPSCS
                     CEEYQCFPMTHIRPDIKTPEDVMNPPKRGKSNQ"
     gene            4616..5218
                     /locus_tag="PKL26_00745"
     CDS             4616..5218
                     /locus_tag="PKL26_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS82254.1"
                     /translation="MGYRIIGLLGSPLPHGNTAKLLDAALKGASDAGCGVEKIEVANL
                     AFRPCREIFYCREHETCAMKDDMTAIYHKFRELDSLIVATPVMTMGIPGALKSFMDRF
                     QVFFMAKYMRKKALVPKERRAHRRGLYLGISGMKVANVFDGAKMTMSAFFSIIDVQYW
                     DDLLVPDMDTIQDITTRPDLLESAYKKGYEMGRLLVEGSA"
     gene            complement(5219..5887)
                     /gene="radC"
                     /locus_tag="PKL26_00750"
     CDS             complement(5219..5887)
                     /gene="radC"
                     /locus_tag="PKL26_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNS82255.1"
                     /translation="MKRMRELDRCDRPREKIAKKGARSLSDQELIAAIIGNGMQGRDV
                     FQVAREIARLFRESADTITYDDLTSIRGVGEGKASQILASMELARRYLKNEEEARVKI
                     QAPADILPLVAGLNGQRQEHFICVTLNGAHEVIATRTITVGLLNHSLVHPREVFADAI
                     TDRAAAVICVHNHPSGTLEPSSQDLAITRQLRDAGDLLGIRLLDHLIVARTGYLSLKE
                     QGHL"
     gene            6090..>6472
                     /locus_tag="PKL26_00755"
     CDS             6090..>6472
                     /locus_tag="PKL26_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNS82256.1"
                     /translation="MATMENAMEAFAGESQANRKYAAFADQAESEGYKNIARLFRAAS
                     EAEAIHAKKLLKAMGKIHATKENLAESVSGETHEFKEMYPAFLKEAEAEKKSDIGLVF
                     TYAMKAEEVHATLYKEALNNLNHGQ"
BASE COUNT         1368 a         1834 c         1855 g         1415 t
ORIGIN      
        1 acatccggta ccgttatccc aggggaatgg aggcgattcg cgggatcagc ttccatgtcc
       61 ggagggggga gaagatcgca ctggtcggtc caaacggtgc cgggaagtcc acaatcctcc
      121 tgatgttcaa tggcatgatt cgtcccgatt cagggaccat actctttgac aatgtgccca
      181 tctcgtacga ccgggcatct ctcaggacgc tccggaagag cgtggggttc gtgctccaga
      241 atcctgaccg gcagatcatc tcccccacgg tataccagga cgtcgcattc ggtccgacca
      301 accttgggtt caccgacgaa gaggtcaagg aggcggtgga gagcgccctg gggcgtgtgg
      361 gcctctctgg gttcgggcgc cgccctcccc accacctctc gggaggagaa aagaagcgtg
      421 tggccatagc aggggtgctc gcgatggacc cggatgtgct ggttttcgat gagccgacaa
      481 gcggcctcga cccgtcgggg tcagaggaca tcatggagct cctcgacgag ctgaaccagc
      541 aggggaaaac ggtcatcatc tcgacacacg acatcgagct cgcctatccc tgggccgacc
      601 gtgccatact cctcctgaat gggcggatcc tccaggaaga cgtcccggat gtcgcttttg
      661 gaaacccgga actcgtgcgg aaggcccgcc tctccatgcc cacggtcctc gaactatacc
      721 tggaactggg ccgaaggggt ttttgcctcc cggagcgccg gccaaagacg gtgctcgata
      781 tgctcaacgt catcgaccgg gccttcagga acaagccctg ctacacacag ccgggcagca
      841 tcaccctctt tgacatcgac tcttcacacg atacggatat cccgggatgg tgcgccgcac
      901 atccgggcgt tcccgtgggg gcaatgggga cccgtgcgaa gcagcgggct gcagacgaga
      961 agatatggct tgacttcacc tatggagtca tcgacaagtg catcctgaag gcgctgctcg
     1021 gtgagcacag cctcattatc gtcacaggga gcatggtggg gagggtccac gagagggttc
     1081 gggagttctg ccgggagaac caggttgaga ttcaggtctc ctctctctca aaaaggcatg
     1141 gatgacgccc cggcctggca ggacccaatg ggttgacaac agttctcatc ttcgcaaagg
     1201 agatacgatc agatgccaat atttgtcaat cctgctgtgt gccatacgtt gattcgaacc
     1261 aggccggcaa ctatgggagc agagagtgac gaggtggtct gatacggctg ctattccttc
     1321 gagggctgac agcgagacgc tcagcgtagc attcaccctg gtctttcggc agggccgggc
     1381 tcctccctcg tgcccgagcc cccgcgaagc agaactctta aaccagatat gcgacagggt
     1441 tcaggcggca tctcctgccg cgtgcaggga tgcactcatc cgcgtgagaa aactctctta
     1501 tgacgtgtat atcgtctgtg atgaattccg ggaagggatt ttcggcaccg ggaatgaggc
     1561 acaggcagcg gcgatcaatg cccttgctga gatcaatccc ggtttttccg cagaggagta
     1621 ccggactgca ttcgtcacag gaatgatgtg gacggcctta tgacaggtca tgccggctct
     1681 tcctcttcct tcaatttttc aaggagcaaa acaacttcag ttcccttctt cctcgcctct
     1741 gcgattgcgg tcgggtgtcc ccggattccc tgatatctgt ccatgtcgtt tgcgatgatg
     1801 ttgtcgtagt actcgaagcc gagagtgttg aaaaatgcgg tgacagccgg gaatgccgcg
     1861 tcaaagacat tgtcccagga gagacctgcg gtggagatga agatgccctt gtgccggcgg
     1921 atctgctcgt ttggaaagta caggttcttc aggatgaatt tcctggccca catgaactgc
     1981 gcccgatcga tgagtccttt cagttccgcg gtgatcccca tactgtagat gggggaggcg
     2041 accgcaaggc attcggccgc aaggatccgg ttgaagagag ggattgcgtc gtctttcacg
     2101 acacactccc ctgtcttgtg gcaggcattg caccccctgc aggggtggta gtcaagatcc
     2161 ttgaggatga tcttctccac ctgcccgccc gctgaatccg cgccctcgag gaaggcgtcg
     2221 aggagggtct ccgtattccc gtgcctgtgg gggcttccgg acaggccgag gacgctgacc
     2281 ggcatgaatt accccccgag cctcttcttc acttcggtga taacctcctt cccgatcctc
     2341 gtggcatcag cctgtgcagt ggggtgttgg aggatggcac ccttctcatc gacattgttc
     2401 accatcaggt agtggatgtc tgcgtccctg acatcgatga cgtggaagaa gcacttgacg
     2461 ctcgggaccg ccccgtcaaa gacatggtcc cagtcctgcc cggcggtgga aaggaatatc
     2521 ccgaggcgct tcccctttcg ttcgggggga acgacaggga gcttgaggac gtatttcctg
     2581 gaccggaaga cctggaaacg gtcgatgagc gccttggcct gggcgcatat ccccatgcag
     2641 aagatcgggg cagcgagaat gatgcagtcg gcctcgatga tcttgtcgtg caggaggtcc
     2701 attccatctc gctgcacgca ccggttcagt ctctcgcagg cattacagcc cctgcagggg
     2761 ttcacgttgg cctcagtaag gggaattttc tcgatgacca ccccctcctc cgctcccatg
     2821 gaagcaagga cccagtcgag caatgtctcg gaattcccgc cccgacgcgg gctcccggca
     2881 aacgcaagga ctttgatctc catcagagag tctgttgacg ctggagatga aaaaagatgt
     2941 gaagtgggga aaaacagacc cgaaaaatgg aggtgactgg ggtcagatat tcttgaagca
     3001 gaggtaccag tccttgcact cgtacccctc ggagagacgc ctgtccatct cctctttgtt
     3061 cttcggtgcc gggaccacca ccttgtcacc gggctgccag ttcgcagggg tcgcgacccc
     3121 gtacttgtcg gttgtctgga gggacttgac gaggcggact atctcaggca tgaaccgccc
     3181 ggcagtcagc gggtaataga tcattgcccg cagtatcccc ttgtcatcga taaagaagac
     3241 tgccctgatt gcagcagtgc tgctctggcc gggatgaatc atcccatact tcttggcgac
     3301 cttcatgtca aggtcggcaa tgatggggaa cgggatatcg acccccatct tctccttcac
     3361 gttcctgatc catgcgaggt gggaatggac cgagtcgatg gagaggccca ttaactggac
     3421 gttgagtgac tttaaatcct ctgcgattgc ggagaatgcg atgaactcgg tagtgcacac
     3481 cggggtaaaa tccgcagggt gcgagaagag cacgagccat ttccccttga aatcagagag
     3541 ctttaccatg ccctgggtgg tgagggcctc aaaatcaggg gccggctcgc cgagaatggg
     3601 cattgaacta aattcaccgc acatttcaat tcactccttc atcagttcat cgagctgcgc
     3661 ctttccatat acgtaggctg gcatgcctgc caggaggaac gcaactctca atgcttcttc
     3721 cacctcgttc tttttaatcc ccaggtttgc ggcacctgca agctgcgccc ttgtggcatg
     3781 ctggtcgcga agcgatgcgg cgatggcgat tgcaatgagc ttttttgtct tccgggtgag
     3841 tgctccgtcg tcccagatgt gcccctcgag cttcatcacc atctcgtaca tcatggggtc
     3901 cttctctgca agttccttga agaacttcgg gaccttgccg atcatcttct caaggtcttt
     3961 gatatttttg gtctcttttg ccatagccgc atcacttctt cagcttcatg ggctggccgc
     4021 agcagacgag ttctcccccg ccgacaacct tcaccacgac gacatttcca cagatctcac
     4081 attcgaactg ctgtccttcc tttgatacat tcaccatgat ccccctccaa gcttaaaaag
     4141 tcccggaaat actatgcatc cgggaaccat ccatattctg aagacgatac cctggacttg
     4201 ctcccgctgt gaagaaaaaa atgaaagtta ctggttgctc tttccgcgct tcggcgggtt
     4261 catcacatct tccggggtct tgatgtccgg gcggatgtgt gtcatcggga aacactggta
     4321 ctcttcacag gagcatgacg ggcattttct caagtcctgc tcgacagcgt cgagcgtcag
     4381 ttcccgtccg cagtccagac agacgtattt gccggggcca actttctgcc cggcttttgc
     4441 cttctcggtc aggtctatca ccaactcggt atatggttca agtcattatt tatggctttt
     4501 gttcttcccc atcaaactcc gatattcggg gtaacacgca cctgtacatc aggctgcagc
     4561 caaatctgag ggcaacaatc cgaagatata tgcgggggcc ctgccaccta actgtatggg
     4621 ataccgcatc atcgggctgc tcggaagccc gctccctcat ggaaacaccg cgaaactcct
     4681 ggatgcagcg cttaaaggcg cttcagatgc cggttgtgga gtggagaaga tcgaggtggc
     4741 aaacctcgcg ttccggccct gcagggagat attctactgc agggagcacg agacctgcgc
     4801 catgaaggat gacatgactg ccatctatca caaattccgt gaactggaca gcctgattgt
     4861 tgcgaccccc gtcatgacga tggggatccc cggggcattg aagtccttca tggaccggtt
     4921 ccaggtcttt ttcatggcaa aatacatgcg gaaaaaagcc ctggtcccaa aggagcgtcg
     4981 tgcccaccgc cgggggctct acctcgggat ttccgggatg aaggttgcga atgtctttga
     5041 cggtgcgaag atgacgatga gcgcattctt ctccataatc gacgtgcaat actgggatga
     5101 tcttctcgta cctgacatgg acaccatcca ggatatcacc acaagaccgg accttctgga
     5161 gtcggcatac aaaaagggct atgagatggg tcggctgctc gtggaaggga gcgcctgatc
     5221 agagatgtcc ctgctctttt aagctcaggt agccagtccg tgcgacaatg agatgatcga
     5281 gaagcctgat accgaggaga tcgccggcat ccctcagttg tcgagtgatg gccaggtcct
     5341 gggaacttgg ctcaagagtc cccgaagggt ggttatgcac gcagatcacc gcggccgccc
     5401 ggtcggtgat ggcgtccgcg aacacctcgc ggggatgaac aaggctgtgg ttcaggaggc
     5461 cgacggtgat ggtccgggtg gcaatcacct cgtgtgcacc gtttaaggtg acgcagatga
     5521 aatgctcctg gcgctgcccg ttcagcccgg cgacgagagg gaggatgtcg gcgggcgcct
     5581 ggatctttac cctggcctct tcctcatttt tgaggtatcg ccgggcgagt tccatcgacg
     5641 cgaggatctg ggatgccttc ccttccccga ccccgcgtat ggaggtcagg tcatcgtagg
     5701 tgattgtatc cgcgctttcc cgaaagagcc tggcaatctc ccttgccacc tggaagacat
     5761 cgcggccctg catgccgttt ccgatgatgg cggcaatcag ctcctggtcc gagagtgagc
     5821 gtgccccctt tttcgcgatc ttctcacggg gcctgtcgca cctgtcgagt tccctcatcc
     5881 gtttcatctg ggatcgcagg ggaagcatgt gcaggggaac cagaaaaatg gcacgcattc
     5941 atacccgaaa gccagggctg ctgttcgaaa taatttgggg ttgcaggcat gtgccaggac
     6001 aagaatatac cgggtggccc cgggatatct cctgctcctg gctggggaga atacctactt
     6061 ttaaggcgcc ctctctcaaa cataccacca tggcaaccat ggagaatgcg atggaggcct
     6121 ttgcaggtga gtcgcaggcc aaccgtaagt atgccgcgtt tgcagaccag gcagaatccg
     6181 aagggtacaa gaacattgcc cgcctcttcc gggccgcctc tgaagccgag gcgatccacg
     6241 cgaagaagct cctcaaggcg atgggaaaga tccacgccac caaagagaac ctggcagaat
     6301 cggtttctgg ggagacccac gagttcaagg agatgtaccc tgcgttcctg aaggaggccg
     6361 aggccgagaa gaagagcgat atcgggcttg tcttcaccta cgcgatgaag gccgaggagg
     6421 tccatgcaac gctctacaag gaggcgctca ataacctgaa ccacggccag ga
//
LOCUS       DAOQVJ010000019         6444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13240_length_6444_cov_3.749413, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000019 DAOQVJ010000000
VERSION     DAOQVJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13240_length_6444_cov_3.749413"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKL26_00760"
     CDS             <1..377
                     /locus_tag="PKL26_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HNS82257.1"
                     /translation="SLTSLYGDSGMARIQPSVVSCTGGYAIIRCIRSTEGQVSACLAA
                     VHQVQEMSVAVRSIRTSGTMRSLRQHLAGMKPRVPCGEQDAYFQGRSWTGVQYPGAKV
                     DLFEKGFKNQQPLFLTEEDLEE"
     gene            384..1109
                     /gene="psmA"
                     /locus_tag="PKL26_00765"
     CDS             384..1109
                     /gene="psmA"
                     /locus_tag="PKL26_00765"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HNS82258.1"
                     /translation="MQPQAYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKC
                     REGVVLIVDKRISSRLLEASSIEKIFKIDEHIGVASSGLVGDARALVDRARIESQINR
                     VTYDEKIDVEALAKKLGDHMQTYTQFGGARPYGTALLIAGVSDGEGRLFETDPSGTLL
                     EYKATGIGIGRNAVMKVFEEEYDFDSTINDSIQLGIRALHDATEGKFDVNMVEIGIIE
                     MENPIFRKMTKEEVAAFMDKFEK"
     gene            1123..1818
                     /locus_tag="PKL26_00770"
     CDS             1123..1818
                     /locus_tag="PKL26_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HNS82259.1"
                     /translation="MIPLEKAVAARLESHGEKFEILVDPDLAVKSRQGEKIELEDMVA
                     ALNVFSNASRGTRASDEALLKVFHTTDFPEIAAKIIQKGEIHLTAEQRKHMIAEKRRQ
                     VINFISRNAINPQTGLPHPPQRIEMAMEEARVNIDLYKHLDELVKETVKALRPILPIK
                     FDELRIAVKIPADYAPRAFGDIQSAATIEREEWQKDGSWICLVRIPAGIQGDFYSLVN
                     RLTKGQGEVKILD"
     gene            1873..2163
                     /locus_tag="PKL26_00775"
     CDS             1873..2163
                     /locus_tag="PKL26_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HNS82260.1"
                     /translation="MSRGQQKAKGRVTGSAGRFGPRYGRFIRKRVVETERISRAAHRC
                     PRCDTISVKRQGTGIWACRKCGFKFAGGAYTPQTPALRIALRAIDRTMEGKE"
     gene            2171..2305
                     /locus_tag="PKL26_00780"
     CDS             2171..2305
                     /locus_tag="PKL26_00780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HNS82261.1"
                     /translation="MAGAYKCARCKQKVEIDVNVRCPYCGHRILFKERGAAIKELKAR
                     "
     gene            2594..2896
                     /locus_tag="PKL26_00785"
     CDS             2594..2896
                     /locus_tag="PKL26_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HNS82262.1"
                     /translation="MPENERACEGEMGGMEHEAVFHFRCPHAETLFRSLSPEMAAELH
                     PRSRAECFMEGQGVLVLKVQARDCAALRAALNMWLRLVNVADEMQALASPAQKERS"
     gene            2893..3255
                     /locus_tag="PKL26_00790"
     CDS             2893..3255
                     /locus_tag="PKL26_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HNS82263.1"
                     /translation="MSSISPKMQNQIAQLQQMQQQMQTIAAQKNQYEMAVREARRATE
                     ELKEVPEENAVFMSVGSVMMQKKKEDILAKLNDKIETLEIRIKSLEKQETMLQGKFEQ
                     LQAQIKQALEGKSSPGAA"
     gene            complement(3269..4807)
                     /locus_tag="PKL26_00795"
     CDS             complement(3269..4807)
                     /locus_tag="PKL26_00795"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNS82264.1"
                     /translation="MWGIVFFVDSPSPRRVTVFDTTLRDGEQTPGISFKFEQKLEIAR
                     QLSSIGVHAIEAGFPASSKGEMETVKAIADLDLDSVVCGLARSRKEDVDACLDCGVNM
                     VHVFIPTSEVQRVHTIKKSCEEVLEITGEIVSYVRDHCDLCMFSAMDATRTDLDYLVQ
                     VFRTAADAGATILNVPDTVGVYTPTAMKALISHLAAEIRCPLDVHCHNDFGLAVANTI
                     TAVEAGASQVQVTVNGLGERAGNADLSSTVMILESIFGMQTGIKKERLVETSRLISRY
                     SGISIPPTYPIVGENAFSHESGIHSHGVIACAATFEPGIMTPEMVGHRRKLTLGKHVG
                     RHAVRQMLEEVHLDPSDSQLDRIVEKIKFISVKGKRVTDADLYEIAESEMGIGNGGRM
                     IEMDELAVMTGNHLIPTASVKAKIGGKEAVYSATGTGPVDAALKAVLGMLPMKVQLKE
                     FSIEAISGGSDAIGHVTIAVEDEQGRIFDASASGDDIVLASAEAMINALNMLHRLDPH
                     TGKK"
     gene            4938..5043
                     /locus_tag="PKL26_00800"
     tRNA            join(4938..4975,4999..5043)
                     /locus_tag="PKL26_00800"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4972..4974,aa:Tyr,seq:gta)
     gene            5077..5149
                     /locus_tag="PKL26_00805"
     tRNA            5077..5149
                     /locus_tag="PKL26_00805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5111..5113,aa:Asp,seq:gtc)
     gene            5244..6296
                     /locus_tag="PKL26_00810"
     CDS             5244..6296
                     /locus_tag="PKL26_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82265.1"
                     /translation="MEIPQLGILSVLALIVIATGVSADIRPPNTAETQSISTLTSVDC
                     IGTFTESDSLVWRQSNEALDYNLVYPLNWPIYVGPGNGNWHYDLDLEEWFFTPGAGEY
                     IFPYPHYPMFAEVPVKEPEPPLAINEVQITSSTRETTMANQGKTAYMKRSSVDTDWKT
                     ANMYNVEQEKIVGFSGLETGRIIAEESALVDAVGMTMTTLEGRVWGCPLCSRSQECLP
                     PFCNVVEMGSSLDMREVQYATHLQSRTIADMAVDGDITGDGVNIRIDLPVVDGPPTEL
                     NYEIEAGGISPGIPARGSVAAYINAHVMEGGDNCPTPCGGPRLDLVYKEKTTASGAVQ
                     AFQKNMYYKSGIKIDI"
BASE COUNT         1501 a         1698 c         1903 g         1342 t
ORIGIN      
        1 aatcgctgac ttcgctctac ggcgattcgg gcatggccag gattcaaccg tcggttgtct
       61 cctgcacagg gggatatgcc attatccggt gtatccgctc cactgaaggc caggtatccg
      121 cctgtctcgc cgcggtgcac caggttcagg agatgagcgt ggctgtgcgg agcatccgta
      181 cctcggggac catgcgctcc ctgcgccagc accttgcggg catgaaaccc agggtaccat
      241 gcggagagca ggatgcgtat ttccagggca gatcctggac aggagtgcag tatccgggtg
      301 cgaaggttga tttatttgaa aagggattta aaaaccagca accattgttc ctcacagagg
      361 aagatctgga ggagtagcga atcatgcagc cacaagcata tcagatgggg tatgaccggg
      421 caatcacagt gttctccccc gacggccggc tttatcaggt cgaatatgcc cgggaagcag
      481 tgaagagagg gaccacggcg gtgggaataa aatgcaggga gggcgtcgtg ctgatcgttg
      541 acaagagaat cagctcccgc ctcctggaag cctcgtctat cgagaagatc ttcaagatcg
      601 atgagcacat cggggtcgcc tcgtcaggcc ttgtcgggga tgccagggca cttgtcgacc
      661 gggcccggat cgagtcccag atcaaccgcg tcacctacga cgaaaagatc gacgtggagg
      721 cccttgcgaa gaaacttggc gaccacatgc agacctacac ccagttcggt ggcgcgaggc
      781 cttacgggac cgcgctcctg atcgcagggg tgagcgacgg agaaggccgc ctctttgaga
      841 ccgacccgag cgggaccctc ctcgaataca aggcgaccgg gataggtatc ggcaggaatg
      901 cggtgatgaa ggtcttcgag gaagagtacg actttgactc gaccatcaac gacagcatac
      961 agctgggaat cagggccctc cacgacgcca ccgagggcaa attcgacgtg aacatggtgg
     1021 agatcggcat catcgagatg gagaacccca tattccggaa gatgaccaaa gaagaagtgg
     1081 cggccttcat ggacaagttc gagaagtaag ggggaatggt tggtgatacc ccttgagaag
     1141 gcggtcgcag cccgactgga gagtcatggc gagaagttcg agatcctggt ggacccggac
     1201 ctggcggtga agtctcggca gggggagaag atcgaactgg aagacatggt agcagcgctc
     1261 aacgtcttct cgaatgcctc gcgggggacc agggcatcgg acgaggcgct cctgaaggtg
     1321 ttccatacca cggattttcc ggagattgcg gcgaagatca tccaaaaggg cgagatccac
     1381 ctcaccgcgg agcagcggaa acatatgatc gccgagaagc ggcggcaggt gatcaatttc
     1441 atctcccgga acgccatcaa tccgcagacc ggtctcccgc acccgcctca gaggatcgag
     1501 atggccatgg aagaggccag ggtcaacatc gatctctaca agcaccttga cgaactcgtg
     1561 aaggagacgg taaaggcgct tcgcccgatc cttcccataa agtttgacga gctccggatc
     1621 gcggtgaaga tacctgcaga ttatgcgccc cgtgcattcg gggacatcca gtccgcggcc
     1681 accattgagc gcgaggagtg gcagaaagac gggtcctgga tctgcctggt caggataccc
     1741 gcagggatcc agggggattt ctacagcctt gtaaaccggc ttaccaaggg ccagggcgaa
     1801 gtgaagatcc tcgattaact atatgaaggc atacagtgaa taataaaaga cacaagagaa
     1861 cgggatgaag atatgagcag gggacaacag aaggcaaagg gaagggttac cgggagcgca
     1921 gggcgcttcg ggccacggta tggaagattt atccgcaaga gggtcgtcga gaccgagcgc
     1981 atctcccgtg ctgcgcaccg ctgtccgaga tgcgacacga tctcggtgaa gaggcagggg
     2041 accgggattt gggcctgccg caagtgcggg ttcaaatttg caggaggagc gtatacgccg
     2101 cagacacccg cgctccgcat cgcgctccgc gcaatcgacc gcacgatgga aggaaaggag
     2161 tgatcctgcc atggcgggag cgtacaagtg cgcccggtgc aagcagaagg tggagatcga
     2221 cgtgaacgtg cgctgccctt actgcggcca ccgcatcctc ttcaaggagc ggggcgcggc
     2281 aataaaggaa ttgaaagcac gctgactggc ctttcctgct actccccttt tttcgaatga
     2341 aaatgcctgc ggcattttca taaatccgta tgtttgtgtc ttgaagagaa tcatgtagtt
     2401 ttatttgatc ttttcacttc gtggagttct ctttcctcaa tacatcgaac catttttatg
     2461 aatgggacct gtatgaggta gtggcatagc cggtcacgct atttgaacag aggcgttttt
     2521 cgcatgtgac caatctgttt aagtgggacc gtgtccaaat aaaaaggtca gaaaaagacg
     2581 aaggggacca gtcttgccgg agaacgagag ggcgtgcgag ggtgagatgg gaggaatgga
     2641 gcatgaagcg gtctttcatt tccggtgtcc ccatgcagag acactgttca ggtcgctgag
     2701 ccccgagatg gctgctgagc tgcacccccg ctcccgggcg gagtgtttca tggagggtca
     2761 aggcgtcctg gtgctgaagg tgcaggcaag ggattgcgcc gcgctccggg cagcactgaa
     2821 catgtggctc cgcctcgtga acgtggcgga cgagatgcag gcgctggcat cgcctgctca
     2881 aaaggagaga tcatgagcag tatatcacca aaaatgcaga accagattgc ccagctgcag
     2941 cagatgcagc agcagatgca gacgatcgcg gcccagaaga accagtacga gatggcggtc
     3001 agggaggcca ggcgtgcaac cgaggagttg aaagaggtcc cggaggagaa tgcagtcttc
     3061 atgagcgttg gctctgtgat gatgcagaag aagaaggaag acatcctggc aaagctcaac
     3121 gacaagatcg agaccctgga gatccggatc aagtccctcg agaagcagga gacgatgctg
     3181 cagggcaagt tcgagcagct ccaggcccag ataaaacagg cgctcgaggg gaaatcatcc
     3241 cctggcgccg cttaaacgat catttcagtc acttttttcc ggtgtgcgga tccagccggt
     3301 gcagcatgtt cagggcattg atcatcgctt cagctgatgc aaggacgatg tcgtcccctg
     3361 atgcgctggc atcgaagatt ctcccctgct cgtcctcgac ggcgatggtg acatggccaa
     3421 tggcatcgga cccgccggag atggcctcga tagaaaactc cttcagctgc accttcatcg
     3481 gcagcatccc gagcacggct ttcagggcgg catccaccgg cccggtcccc gtggcactgt
     3541 agactgcttc ttttcccccg atcttcgcct tcacgctcgc ggtgggaatg aggtggttgc
     3601 cggtcatcac tgccagttca tccatctcga tcatccggcc gccgttcccg atccccatct
     3661 cgctctcggc gatctcgtag aggtcggcgt ccgtgacccg ctttcctttc acggagatga
     3721 acttgatctt ctccacgata cggtcgagct ggctgtcgct cgggtccagg tggacctctt
     3781 cgagcatctg gcgcaccgcg tgcctcccga cgtgcttgcc gagtgtcagc ttccggcggt
     3841 ggcccaccat ctcgggagtc atgatgccgg gctcaaacgt ggcggcacag gcaatcacgc
     3901 cgtgggagtg gatcccgctc tcgtgtgaga aggcgttctc cccgacaatt ggataggtgg
     3961 gcgggatcga tatcccggaa taccgggaga tcaaacgcga ggtctccacc agcctctcct
     4021 ttttaatccc ggtctgcatc ccgaagatgg actcgaggat catcacggtc gaagagaggt
     4081 ccgcgttccc ggcacgctca ccaagcccgt tcaccgtcac ctgcacctgg gaggcgccgg
     4141 cctcgacggc ggtgatggtg ttggcgaccg caaggccgaa gtcgttgtga cagtgcacgt
     4201 cgagcggaca cctgatctcg gctgccagat gcgagatgag ggccttcatt gcggtcgggg
     4261 tgtagacccc cacggtgtca gggacgttga ggatggttgc cccggcatcc gcggcggtgc
     4321 gaaagacctg caccagatag tcaaggtctg tccttgttgc gtccatcgcc gagaacatgc
     4381 acaggtcgca atggtcgcgg acgtaggaga ctatctcgcc ggtgatctca agcacctctt
     4441 cgcagctctt cttgatggta tgaacccgct gcacctcgga ggtaggaatg aagacatgca
     4501 ccatgttcac cccgcagtca aggcaggcgt ccacgtcctc tttcctagac cgggccaggc
     4561 cgcagaccac agaatcgaga tcgaggtccg cgattgcttt taccgtctcc atctctcctt
     4621 tcgatgaggc cgggaaccct gcctcgatgg catggacccc gatggaggag agctggcggg
     4681 ctatctcaag tttctgctcg aatttgaagg agataccggg tgtctgttca ccatccctga
     4741 gggtggtatc gaaaacagtg actcttctag gcgacggact atcgacaaag aagacaattc
     4801 cccacatcag tggttctgga ggtcatacct ttattttacg ttgtccgtac tataaaaaag
     4861 tacgcggccg ggcaatacca ttcccgccaa atcgatacgt ttaataggtt tattccccct
     4921 atagtatgag cgcaaatccc gccttaactc agactggtag agtgcgcggc tgtagtatga
     4981 tctgccgttt caggcaattg cgcggctacc gcgatgtccc cggttcaaat ccgggaggcg
     5041 ggatctcaaa atcagatgcg cgaaaaggac tggtgtgccc aggtagtgta gcggcctatc
     5101 atgtcggcct gtcacgccga cgactcggat tcgaattccg acctgggcgt tcttgtttta
     5161 ttttgggttt ttttgcagaa tagtgaaata tatataatcc cttaccgaaa ataacgaaca
     5221 ataattcatt ggggactgaa ggaatggaaa tacctcaatt gggcatactc tctgttctgg
     5281 ccctcattgt aattgctaca ggtgtgtcag cagatatccg gcccccaaat accgcggaga
     5341 cccagtcgat ctctactctc acttccgttg actgcatcgg aacgttcact gagagcgatt
     5401 cgctggtgtg gcggcagagc aacgaggcgc tggattacaa cctcgtatat cctctcaact
     5461 ggccgatata tgtcgggcct ggaaatggaa attggcatta tgatctcgat ctagaagagt
     5521 ggttttttac tccgggcgcg ggcgagtata tatttcctta cccccactac ccgatgtttg
     5581 ccgaggttcc tgtcaaagaa cccgaacccc ctcttgccat taatgaagtc cagattacca
     5641 gctctacccg cgagacaacg atggcgaacc agggaaagac ggcctacatg aagcgttcat
     5701 cggtggatac tgattggaag actgcgaata tgtataacgt ggagcaggag aagattgtgg
     5761 gattttcagg tttggagacc ggccgaataa tagctgaaga atcagcgctg gttgatgcgg
     5821 taggaatgac catgaccacc ctggaaggca gggtctgggg atgcccgctt tgctctcgtt
     5881 ctcaagaatg tcttcctcct ttctgcaatg tggtagagat gggaagctca cttgacatga
     5941 gagaggtgca gtatgccacc cacctgcaat cgcggaccat cgcagatatg gcagtggatg
     6001 gagatatcac aggtgacgga gtgaatatcc ggatagactt gccggtcgtt gacggccctc
     6061 cgactgaact caattacgag attgaggcag gcggaataag cccgggaatt cctgcacgag
     6121 gatccgttgc ggcatatatc aatgctcatg ttatggaggg aggagataat tgtcccacgc
     6181 catgtggtgg acctcgcctg gatttggtat acaaggaaaa aactactgca tccggcgcag
     6241 tacaggcttt ccagaagaac atgtattaca aatcaggtat taaaattgat atctgattat
     6301 cccaaaacac tctttctggt attttttatc ggtttcgtta acgatgaaca ctcctggtaa
     6361 tgcgctggag ggcacactcc accgcaagcc ttgcacaggg atcctcgtcg tcaagcatcc
     6421 gctcaaggta ggggagtgct ttcc
//
LOCUS       DAOQVJ010000020         6440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13252_length_6440_cov_4.589193, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000020 DAOQVJ010000000
VERSION     DAOQVJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_6440_cov_4.589193"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKL26_00815"
     CDS             complement(<1..295)
                     /locus_tag="PKL26_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNS82266.1"
                     /translation="MQETPAAEGTGRRRNQLLVVLVLAGAVICAAASFILFTTYAKDL
                     GISVVRVEGMIVTGNSRGDGYVGSEYVGRMIREAADDPIVDAIVIRVNSPGGTP"
     gene            complement(273..989)
                     /locus_tag="PKL26_00820"
     CDS             complement(273..989)
                     /locus_tag="PKL26_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNS82267.1"
                     /translation="MPEPKRRPHVLMMSEITVDGKLTLKKGASSKILMKYMDPATEIL
                     LHKTRAACDAIMVGSNTIRIDNSFLTVRHVEGKSPLRVIPSSMADIPPDANVLGPGAP
                     TVVAVAARAPQERVEAIRRRGVHVLVSGEERVQLPLLMEQLFDQFSVRTMMIEGGPTL
                     NWHMLHHRLVDEIRLIHLPFIVGGADTPSLVGGMHIEDESEMICLTLKNHYLCGTNLV
                     TEYQVRYNPGGAGDAGDSGR"
     gene            1159..1791
                     /locus_tag="PKL26_00825"
     CDS             1159..1791
                     /locus_tag="PKL26_00825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNS82268.1"
                     /translation="MNITALRPRFVRYLEPVAAFFCRIGATPNQVSLLALFFGLLCAY
                     LFFRQMFLAGSLALIISAVLDLVDGAVARQTSRQTQFGAVFDWIVDKYVDALVLLGIG
                     LSGIAIITRFTPLPPLADFAVVAFAITGSLMNTFIKPVVYAEIGYQERVDGKIEDPLE
                     GVGFFGRPETFLVLLAGGLTGFIWLSVLVIAICTNLSALQRIIYLYQTLS"
     gene            complement(1763..2569)
                     /gene="artA"
                     /locus_tag="PKL26_00830"
     CDS             complement(1763..2569)
                     /gene="artA"
                     /locus_tag="PKL26_00830"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HNS82269.1"
                     /translation="MKELLVLFSCLFFLAFLVPWKLRNWCAVFGWTCIVLFLFAEVPY
                     YFSINNFLYPVMAVLSVPFLVITARHLITREVLVMQLSTAAAVAFLIYAPFAYSPLGD
                     WLISVVVGQTAWMLEAFGYPPLQLAWNMLSRNNFRVEIILACTGIQAIAIMLGVVSAV
                     KTTPRQKVLSFLLIVPTIYILNIVRNTAVIIAYTEQWFPFLPDIAGNGEFGYESFFWA
                     HNVLAEGLALLLLIGIAWGLFRLIPDLADFATGLVQLYWRDIRRAFGKDR"
     gene            2707..3018
                     /locus_tag="PKL26_00835"
     CDS             2707..3018
                     /locus_tag="PKL26_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HNS82270.1"
                     /translation="MFCPECRSLMISSAGQLKCRKCGYIRNIGKDDQMQIRKERRERE
                     IVIVEEEGSVRTLPTIATKCPKCEHNLAFWWLRQLRAADESEVRFFRCCECGHTWREY
                     D"
     gene            3233..3886
                     /locus_tag="PKL26_00840"
     CDS             3233..3886
                     /locus_tag="PKL26_00840"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HNS82271.1"
                     /translation="MDASYFFTQFAAYNTDWLPVNVILWLLLLITTALVFMRSADMRS
                     QALIKGALALVFLWNGIVFFFFYMTLSAVPGGIPMVVIGLLFATDIFRKRITISLPAS
                     GWVRYATLAWAIWALGLYTIAGWLTGHPYPGGPLPAAPCPTTILAIALLSTSMATLKT
                     TRLLFTLLFALLLWWAFFAGTFAPAMYGFYLDLTLLAAGVYGLVMILKNRPNAGVMG"
     gene            complement(4215..5081)
                     /locus_tag="PKL26_00845"
     CDS             complement(4215..5081)
                     /locus_tag="PKL26_00845"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNS82272.1"
                     /translation="MQNRKDAGVNEVIASILMVALVIVLAIIIGSIVFGVIVIQPKSA
                     YIPPRVEVVNYSGQQAISLYSRGGDPAVLDKGDGVQYALGIYVENTSGSSRATPDPGV
                     KSFGPGQTLYIYYRNGEYRATDNLTGKTFDKLPPGPLTLRLVDENARLLVYKEGIGMG
                     GEGTVTVSPTPTPSCGTITGIVFNDLNGNGQRDPGEPGLSGWTVQYRYSKNLGQGSGS
                     GDWSAWISGTTGSDGAYTFSSLLYQPTHFYEVRVLLQQGYTTIKPQLVYSFKLTPNEN
                     ECYATGKDFGIS"
     gene            5295..5855
                     /locus_tag="PKL26_00850"
     CDS             5295..5855
                     /locus_tag="PKL26_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HNS82273.1"
                     /translation="MKVKPEESLKIENIVASAKVTDYLDLPALASQIKDAEYNKKRFP
                     GVVIRMQNPKIAALVFGSGKVVLTGAKSIESLSQGLEILGNLLRGLQIDIPKKLSYKI
                     QNIVTSADLGSGINLNKIAVGFNLDRIEYEPEQFPGLVYRLENPKVVVLLFGSGKLII
                     TGGKEPEDARKAVQKILTDLKNLGLL"
     gene            5964..6338
                     /locus_tag="PKL26_00855"
     CDS             5964..6338
                     /locus_tag="PKL26_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS82274.1"
                     /translation="MKSRNVMYFTPREEEFADLLVRIGLKKNVAKVLVYLAHTPEATS
                     RDIERGTDLRQPEVSIAMATMMDQRWVENRETKAENKGRPVKIYRLSQPVGEIMDTIE
                     KEKRAEATNQLNLIQQLRDYIS"
BASE COUNT         1370 a         1854 c         1762 g         1454 t
ORIGIN      
        1 taggggttcc gcccgggctg ttgaccctga tcacgattgc atcaacgatc gggtcgtctg
       61 cggcctccct gatcatgcgg cccacgtact cggaacccac gtacccgtcc ccgcggctgt
      121 tccctgtcac gatcatgccc tccaccctca ccacggagat gccaaggtcc tttgcatagg
      181 tggtgaacag gataaatgat gcagccgcgc agatgaccgc gccggcaagg acgagtacaa
      241 cgagtaactg gttccttcgc ctgcccgtcc cttcagcggc cggagtctcc tgcatccccg
      301 gcacctcccg ggttgtaacg gacctggtac tcggtcacca ggttggtccc gcagagataa
      361 tggttcttta aggtcaggca gatcatctcg ctctcgtcct cgatgtgcat tcccccgaca
      421 agcgaggggg tgtctgcccc cccgacgatg aaggggaggt ggatgagccg gatctcatcc
      481 acgagccggt ggtgcagcat gtgccagttg agggtaggac caccctcaat catcatggtg
      541 cggacagaga actgatcaaa gagctgctcc atgaggaggg ggagttgcac ccgctcctcg
      601 cccgagacca gcacgtggac tccccgcctt cggatggcct ccaccctctc ttgcggggcc
      661 cgggcggcca cggccaccac cgttggggca cccggcccga ggacgttcgc atccgggggg
      721 atatcggcca tgctgctcgg gatgacccgc agggggcttt ttccttccac atgcctgacc
      781 gtgaggaacg aattgtcgat tcggatggta tttgagccca ccatgatggc atcgcaggcc
      841 gccctggttt tatggaggag gatctcggtc gcagggtcca tgtacttcat caggatcttg
      901 ctcgatgccc cctttttgag ggtcagtttc ccgtccacgg tgatctctga catcatcaag
      961 acatgcggtc gtcgtttcgg ttccggcaag gcagattcag tctcctgata gactcatatc
     1021 ttgttgctct catatagaag ttccctggct ctgcacgaga agaattgatt cgcgattcct
     1081 ccaaaacaga ggatatgcgc cctgcgatac gatgcgggat gggaatcatt attggtccac
     1141 ccggcatggt acttaggcat gaatattact gctctccggc caaggttcgt gcgttacctc
     1201 gagccggtgg cagcattctt ctgccggatc ggcgcaaccc caaaccaggt gtcccttctt
     1261 gcactctttt tcgggctcct gtgcgcctac ctcttcttca ggcagatgtt tttggccggg
     1321 agccttgcac tcatcatctc cgccgtcctc gacctggtgg acggtgcagt ggcccggcag
     1381 acctcaaggc agacgcagtt cggggcagtc tttgactgga tcgtagacaa gtatgtcgat
     1441 gccctggtgc tgctcggtat tggtctctcc ggcatcgcca ttatcacaag gttcactccc
     1501 ctccctccac tggcagactt tgcggtggtt gccttcgcga tcacaggttc gctgatgaac
     1561 acgttcatca agccggtggt ctacgccgag atcggctacc aggagcgggt ggacgggaag
     1621 atcgaggacc ccctggaagg agtggggttc ttcggcaggc cggagacgtt tctcgtcctc
     1681 cttgcgggcg ggctgaccgg gttcatctgg ctctcggtcc tggtgattgc catctgcacc
     1741 aacctctccg cactccagcg gatcatctat ctttaccaaa cgctctcctg atgtcacgcc
     1801 agtagagctg cacgaggccg gtcgcaaaat ccgcgaggtc ggggatgagc cggaagaggc
     1861 cccacgcgat cccgataagc agcaggaggg caaggccttc agcaaggacg ttgtgggccc
     1921 agaagaagct ctcgtacccg aactcgccgt tcccggcgat atccgggagg aaggggaacc
     1981 actgctcggt gtaggcgatg atgaccgcgg tgttccggac gatgttcagg atatagatgg
     2041 tgggaactat caggaggaac gagagcactt tctgccgggg ggtcgtcttc accgcgctca
     2101 cgacgccgag catgatggct atggcctgga tgccggtgca ggcaaggatg atctccaccc
     2161 tgaaattgtt ccgggagagc atgttccagg caagctggag gggaggatac ccgaaggctt
     2221 cgagcatcca cgcggtctgc ccgaccacca cagagatgag ccagtcacca aggggagagt
     2281 aggcaaacgg cgcatagatg aggaaggcca ctgctgccgc ggtggagagc tgcatgacca
     2341 gcacctcgcg tgtaatgagg tgccttgcgg tgatcacgag gaacgggacc gagagcaccg
     2401 ccataacggg atacaggaag ttgttgatgg agaagtaata ggggacttct gcaaagagaa
     2461 agaggacgat gcaggtccat ccgaacaccg cacaccagtt gcggagcttc catggcacaa
     2521 ggaacgccag gaaaaacagg caggagaaga gtaccaggag ctccttcatc cctgttattt
     2581 tggcgtccat ggatgaaaaa gagtgcgttt gcacaccctt cccgccgcat cgggcaaaat
     2641 atgcggagaa ttcacattac tacaaaccaa gccttaatct cgaatggtaa cgcataatac
     2701 cccatcatgt tctgtccgga gtgcaggagc ctgatgatct cctctgcagg ccagttgaaa
     2761 tgccggaaat gcgggtatat ccgcaatatc ggcaaggacg accagatgca gatcaggaag
     2821 gaacggcgcg agagggagat cgtcatcgtc gaggaggagg ggtcggtacg cacgctcccc
     2881 accatcgcga ccaagtgccc caagtgcgaa cataatctcg cgttctggtg gctccggcag
     2941 ctccgtgccg ccgacgagag cgaggttcgc ttcttccgct gctgcgaatg cggccacacc
     3001 tggcgcgaat acgactgatc ttttctattc cttcccttct ctccaggggt ttttggtgcc
     3061 tcttcacatc ccggcgaccg cacggtcgcc ccggggtcga ggggcggcag ccccccgccc
     3121 tgcaaaactc gcgttctggt ggctcaccca ggttcatcag gtattcactt ttattctcaa
     3181 tcacggccat catatggtta taccgcacga ccgggtggga tgaggttaca ctatggatgc
     3241 atcctatttc ttcacgcagt ttgctgccta caataccgac tggctccctg tcaacgtaat
     3301 attgtggctg ttgctcttga tcaccacggc tttggtcttc atgagatccg cggacatgcg
     3361 atcgcaggca ctcatcaagg gcgcactggc gctggtcttc ctctggaacg gcatcgtctt
     3421 cttctttttc tacatgacgc tgtccgcggt tcccggaggg atacccatgg ttgtgatagg
     3481 gttgctgttc gcaaccgaca tttttcgaaa aagaatcact atctcactgc cggcatccgg
     3541 atgggtccgg tatgccaccc tcgcctgggc catctgggcg ctcggactct atacgatcgc
     3601 cggatggctg accggccatc cctaccccgg gggaccgctt cctgccgcac catgtcccac
     3661 cacgatcctg gcaatcgccc tcctctcgac atcgatggcg acgctgaaga cgacccgtct
     3721 tctcttcaca ctcctgttcg ctctcctcct ctggtgggcg ttctttgccg gcaccttcgc
     3781 ccctgcgatg tacggctttt acctggacct tacgctgctg gccgctggcg tgtatggact
     3841 agtcatgatc ctcaagaacc ggcccaatgc aggtgttatg ggatgatgtg aagggaggag
     3901 gatggcactt tttcttttct gcattggtag tcctccctac ccgttgtttt acctgatcag
     3961 atgaggaatc tgtcaggtac cgaccccatt gtaaaatcgc ttccattatg gaataaaaat
     4021 gagcatactg atagacaata tcacaaaaat ctacagtcct ttctattcag atatgctact
     4081 attccatgcg gactatcgcg ttttggggga ggggacaggg gagggggatt ctccccctcc
     4141 ctcctgtggg aattattgtc tttctcctga aactcttcag gtaaaaagga tgaaattttt
     4201 tatagaggtg aattttaact gataccgaag tctttcccgg ttgcgtaaca ttcattttca
     4261 tttggagtca gtttaaacga atacactaat tgaggcttaa ttgttgtata accctgttgt
     4321 aaaagcactc tcacttcata aaaatgagtt ggttgataga gtaaactgga gaaggtatat
     4381 gctccatctg atccggtcgt gccacttatc catgccgacc agtcgcccga tcccgagcct
     4441 tggcctaggt ttttactgta ccgatactga acagtccatc cgcttaaccc gggttctcca
     4501 gggtctctct ggccatttcc attcaaatca ttgaacacaa taccggtaat tgtgccacat
     4561 gagggtgtag gagtcggact caccgttaca gtcccttctc ctcccatccc aatcccctcc
     4621 ttatacacaa gcagcctcgc attctcgtcc actaggcgca gggtaagcgg accagggggg
     4681 agcttatcga acgtctttcc ggtcaggttg tctgttgcac ggtattcccc attgcggtaa
     4741 tagatataca gtgtctggcc tgggccaaac gacttgaccc ccggatctgg tgtggcacgc
     4801 gatgatccgc tcgtgttctc gacgtagatg ccaagcgcat actgcacgcc atctcccttg
     4861 tcaaggaccg cagggtcccc accccgcgag tagaggctga tggcctgctg gccgctgtag
     4921 ttcaccacct cgacccttgg cgggatatag gccgacttcg gttggatcac gatgacgccg
     4981 aacacgatgc tcccgatgat gatggccagg acgatgacga gcgccaccat gaggatggat
     5041 gcgatcacct cgtttacacc ggcatccttc cggttctgca tacttagacc tgcgggagaa
     5101 aggtgataaa accgtccatg ggtcactcac ccggtttcct gggttttgcc atacccgtgc
     5161 agccagccga tcacttcgat cacggacatc atgggagtcc tgatcttcct ctcgatcgcg
     5221 gcgttcctga tcgacccgtg accctggggg tcgcacccaa ctgtttatat actgtccggg
     5281 ggcattttat aaacatgaag gtcaagccag aggaatctct caagattgaa aatatcgttg
     5341 cctcagccaa ggtgaccgat tacctggatc ttcccgccct ggcctcccag atcaaagatg
     5401 ctgaatacaa taagaagcgg tttcccggag tcgtcatcag gatgcagaac ccgaagatcg
     5461 ccgcgctggt ctttggttcc gggaaggtgg tgctgaccgg ggcgaagagc atcgagagcc
     5521 tctcgcaggg gctggagatc cttggaaacc tcctccgcgg gctgcagatc gacatcccaa
     5581 agaaactgag ctataagatc cagaacatcg tcacttccgc ggacctcggc tcaggcatca
     5641 acctgaacaa gatcgccgtc gggttcaacc ttgaccggat cgagtatgag cccgagcagt
     5701 tccccggcct tgtataccgg ctcgagaacc cgaaggtcgt ggtcctcctc ttcgggtccg
     5761 gcaagctgat catcaccggc ggcaaggagc ccgaggacgc gaggaaggcc gtgcagaaga
     5821 tcctcacgga cctcaagaac ctcggtctcc tctgatacca ttttttttgt ttttctcccg
     5881 tttccttttt ttaatgctat ataaataata ataatctttt tatataatat atgttaataa
     5941 cataagaaga gtcggtgaac accatgaaat cgagaaacgt catgtacttc acccccaggg
     6001 aggaggaatt tgccgatctc ctggtgcgga taggattgaa gaaaaacgtc gcgaaagtcc
     6061 ttgtatacct ggcccacacc cccgaggcaa cctcgaggga tatcgagcgg ggcaccgacc
     6121 tccgccagcc tgaagtgagc atcgccatgg ccacgatgat ggaccagcgc tgggtggaga
     6181 acagggagac caaggctgag aacaagggca ggccggtgaa gatttacagg ctctcgcagc
     6241 ctgtcggcga gatcatggac accatcgaga aggagaagag ggctgaggcc accaaccagc
     6301 tgaacctgat ccagcagctg cgggactata tctcctgatc ccgtccccct ctgattaccc
     6361 tgcagcccct ctcttctccg atcacaagcg tggtcttttc cgtgtagcgg cagaagagcc
     6421 cgcattccac aactcccggg
//
LOCUS       DAOQVJ010000021         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13268_length_6433_cov_4.642364, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000021 DAOQVJ010000000
VERSION     DAOQVJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13268_length_6433_cov_4.642364"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKL26_00860"
     CDS             complement(<1..398)
                     /locus_tag="PKL26_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNS82275.1"
                     /translation="MKDEVMDKYIEAGKIASEIRRKGAGEIRVGASYLAVVDTIEEMV
                     RDAGAGLAFPLNVSLNEDAAHDTASAGDERVFAQGDVVKLDLGVHVDGYIADTAMTVD
                     LGDHPLLIEASARGLASAISMVAPGVTVGEL"
     gene            complement(410..1537)
                     /locus_tag="PKL26_00865"
     CDS             complement(410..1537)
                     /locus_tag="PKL26_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNS82276.1"
                     /translation="MDALEEAILRSGSTAYVAYGSSQDADMRYLTRFSTTDPIVYIRK
                     PGEKGTVVVSQMEYERAEREATAAVISRAQAGLFEILKGEQDRWRALARMIAGLVQGK
                     VLIPPQFPYALGRELSELVAVEVDREALARMRAAKSPWEIGRISAVQAATEEAMDRGI
                     QVIRKARVKGGLLYMGAVPLTSGMVRAAMHQVLQKRGCIPTDTIVSCGEESALPHLRG
                     EGALKADEPIVIDIFPRDEQTGYYSDMSRTVCSGEPSPDVLEMFQAVRDAQDLAAETI
                     RPGVTGEEVYRAVVGFFEETGYGTGSSGFVHNLGHGVGLEVHEAPSLGPSGEVLLPGN
                     VVTNEPGLYMPGIGGVRLENIGVIGPGGFATLTRYPRDLAV"
     gene            1599..1856
                     /locus_tag="PKL26_00870"
     CDS             1599..1856
                     /locus_tag="PKL26_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82277.1"
                     /translation="MDEATKEQLKWKFYRLAIILNAIVLLVALGVIAILKLPEPVALP
                     GGIALILLAVGLAIYFWKKYVSTKKWLDEQASKDHAGEQGQ"
     gene            1853..2143
                     /locus_tag="PKL26_00875"
     CDS             1853..2143
                     /locus_tag="PKL26_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82278.1"
                     /translation="MIEKVKGEIELMQRHIQVLRTVVAHQPIGIMKLSELMDLPFHRI
                     RYSLRVLEQMGYIRASQSGAVATPQSVELLAGLDQELGEMIRLLESMRHQGG"
     gene            2240..2312
                     /locus_tag="PKL26_00880"
     tRNA            2240..2312
                     /locus_tag="PKL26_00880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2273..2275,aa:Asn,seq:gtt)
     gene            2326..2400
                     /locus_tag="PKL26_00885"
     tRNA            2326..2400
                     /locus_tag="PKL26_00885"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2361..2363,aa:Ile,seq:cat)
     gene            2484..3512
                     /locus_tag="PKL26_00890"
     CDS             2484..3512
                     /locus_tag="PKL26_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82279.1"
                     /translation="MKCPVCGDGCIQDAPAVLESLASVFSRCPRCTFRVLDKSAPLKG
                     RYYGTPCECGKRFIDEVFAHLYVILVEEGIFSGSEPLRAVGSPLLHPGFFIAGPPFLP
                     PGSLVLLSPLVTESAARRMITEVPEVRGVVRSGDFVPGATDPSLATVPRSYELLAGCD
                     VRAGIFPTGAGPLVIYTQQSQMHIEFPRLENPKIQSVERHIAAQRPTWCVDACSGAGT
                     LGLCAARHGIPGVILNDAWYSAAFWSATNLHVNRMFFQVEGVRIHLSFREISMHPVGR
                     DPVKVAEASGGQSIAVYHGDFRQLWKVLPREPVCAVLDLFEKSDTRMTEAAMKEWQSR
                     VPGEVFIP"
     gene            3527..3839
                     /gene="ffs"
                     /locus_tag="PKL26_00895"
     ncRNA           3527..3839
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKL26_00895"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            3860..4807
                     /locus_tag="PKL26_00900"
     CDS             3860..4807
                     /locus_tag="PKL26_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNS82280.1"
                     /translation="MSKVTIVGATGNVGTFSAHTLAGIPYVHEILLHGRAGREDLLCG
                     ITQDLIDSFAASGQAVDLSWTTDIMDARDSDIVVCTAGVPRRPGQDRLDLALQNARIV
                     ADTARVIGKCAPHAMILMVTNPVDVMTSVALRYSGMQPNQVFGLGTHLDSMRLKSLIA
                     KHFHVHVSEVHTRIIGEHGDSMVPLWSATTIGGIQISNLPAFSRLPVEEMVATVKSSG
                     EQIIRNKGATVYGPGEAIATLVRTVLGNEDRILTVSAYIRSEVHDIGDVCIGVPARIN
                     RSGVFPVPIKIAEDEVAQFGVSVDKIRAVTHEILQKLRE"
     gene            complement(4859..5116)
                     /locus_tag="PKL26_00905"
     CDS             complement(4859..5116)
                     /locus_tag="PKL26_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HNS82281.1"
                     /translation="MGTVAIILKVMPESPEVDLAVLKREIQEKVPSVRDIREEPIGFG
                     LKALKVAAIVNDAGGESDAVEAEIQKISGVERAEIVELTLT"
     gene            complement(5120..5281)
                     /locus_tag="PKL26_00910"
     CDS             complement(5120..5281)
                     /locus_tag="PKL26_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNS82282.1"
                     /translation="MPIRKCTSCNSPLAEEGAAEFGCPKCDYQIRRCYRCREQSIPYT
                     CPKCGFGGP"
     gene            complement(5328..5921)
                     /locus_tag="PKL26_00915"
     CDS             complement(5328..5921)
                     /locus_tag="PKL26_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HNS82283.1"
                     /translation="MTPVRPPLVLKIGGSLQDCAGSICALLVDLDVPVLVIPGGGAFA
                     DLVRECRLEGTAAHWMAVSAMEQYGWYLSSFGLPVTTRLDIPGGPAILLPYGLIRAID
                     PLPHRWEVTSDTIAAWCAHTLHLPLLLLKSVDGIRVEGRLCPRIRSPVETDDVDSCFL
                     PFVLRRRMHALVISGRHPDRLRAALTGLPTIGTTIGF"
     gene            5982..>6433
                     /locus_tag="PKL26_00920"
     CDS             5982..>6433
                     /locus_tag="PKL26_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNS82284.1"
                     /translation="MIRTTIMLLLVLAAPVTGIELVEFCPDPYLPDDRDEYLCLEGWG
                     QADGILVTDGEGGIRFPPGTTLRGLTVVAMDAEAYRNSHGEYPDFEWDGSSPVVPDMI
                     RTGRFQLSNTADEIEVFSGRVCIQKVDWPGTVSPRQGQVHSFSEGMWD"
BASE COUNT         1255 a         1930 c         1922 g         1326 t
ORIGIN      
        1 agttctccca cggtgacgcc cggcgcgacc atcgagatcg ccgaggccag tccgcgcgct
       61 gaggcctcga taaggagcgg gtggtcaccg aggtctaccg tcatcgcggt atcggcgatg
      121 tagccatcta cgtgaacacc gaggtcgagc ttgacaacgt ctccctgtgc aaataccctc
      181 tcgtcaccgg ctgaggcggt atcgtgcgcg gcgtcctcgt tgagagagac attcaggggg
      241 aatgcaaggc cggcaccggc gtcccgcacc atctcctcga tcgtatccac tacggcaagg
      301 tatgacgccc ccaccctgat ctcgcctgca cctttcctgc gtatttccga ggctatcttt
      361 cctgcctcga tgtacttgtc catcacttcg tctttcatta ggtcccccct cacactgcca
      421 ggtctcttgg gtaccgggtt agggtcgcaa acccgcctgg gccgatgact ccaatgttct
      481 caagccgtac gccgccgatc ccgggcatgt agaggccggg ctcgttggtg accacgttcc
      541 ccggcagcag aacctctccg gagggcccga gactcggtgc ttcgtggacc tcgaggccca
      601 cgccatgccc gaggttgtgc acgaatcccg agctgccggt cccgtatcca gtctcttcga
      661 agaagccgac caccgcgcgg tagacctctt caccggtgac gccggggcga atcgtttctg
      721 cggcgaggtc ctgggcgtcc ctgaccgcct ggaacatctc aaggacatcg ggggatggct
      781 ctccactgca gactgtacgg ctcatatcgc tgtagtagcc ggtctgctcg tctcttggga
      841 agatgtcgat cacgatcggt tcgtctgctt tcagcgcccc ctctcccctg agatggggaa
      901 gtgcgctctc ctcgccgcag gagacgatgg tatcggtagg gatgcaccct ctcttttgga
      961 gaacctggtg catagccgcc cggaccatcc ctgacgtgag ggggaccgct cccatgtaga
     1021 gaagcccccc cttcaccctg gccttcctga tcacctggat cccgcggtcc attgcctcct
     1081 cggtggcagc ctgcaccgca ctgatcctcc ctatctccca cgggcttttt gctgccctca
     1141 tcctggcgag tgcctccctg tccacctcga cagccacaag ttccgagagt tctcttccaa
     1201 gggcatacgg gaattggggg gggatcagca ccttgccctg cacgaggcca gcgatcatcc
     1261 gggcaagcgc ccgccatcgg tcttgctccc ctttcagtat ctcgaacagc ccggcctggg
     1321 cccgggaaat cacagcagca gttgcctctc tctcggcccg ttcgtattcc atctgtgaca
     1381 ccacgacagt ccccttctcc ccgggtttgc ggatatagac aattggatct gtagtggaaa
     1441 accgggtgag atagcgcatg tcggcgtcct gagatgagcc ataggccacg taggccgtcg
     1501 acccgctccg caggatcgcc tcttcgagcg catccatatg ccatccttat tggaggcgat
     1561 cccacaagta cttttatcat cgcgcagata ttcctgtaat ggacgaggcc acaaaagagc
     1621 agttaaaatg gaaattttac cgcctggcga tcatcctcaa tgcaatcgtg ctgctggtcg
     1681 cgctgggagt catcgcaatc ctgaaactcc cggaaccggt ggcgcttccc ggagggattg
     1741 ccctcattct cctggcagtg gggctcgcaa tctatttttg gaaaaagtat gtctccacca
     1801 aaaagtggct cgatgagcag gcctcgaagg atcatgccgg ggagcagggt caatgattga
     1861 gaaggtgaaa ggagagatcg agctgatgca gcggcacatc caggtgctgc ggacagtggt
     1921 ggcccaccag cccatcggca tcatgaaact ctcggagtta atggacctcc ctttccacag
     1981 gattcgctac tccctccgcg tgcttgagca gatgggatat atccgggcat ctcaatccgg
     2041 tgcggttgca acaccccaga gcgtggagct cctcgcgggc cttgaccagg agcttggcga
     2101 gatgattcgt ctccttgaaa gcatgagaca ccagggcggc tgatacgcga aaagtccggc
     2161 agttcccatt gtccacccct gcatgaatct ccgctacatt taacaccgaa agcggcaacc
     2221 tatattggca catcggattg ccgccataac tcagttggta gagtggctgg ctgttaacca
     2281 gcatgtcaca ggttcgagtc ctgttggcgg cgttttttct tccatgggcc cgtagcttag
     2341 tccggtcaga gcgcccggct cataaccggg cggtcatgcg ttcaaatcgc atcgggccca
     2401 ctttaaatcc ctcccgtcct ggcacccgcc attgccgccg tgttcaaaaa ggttagtatg
     2461 gggtccggtc caatttccca ggtatgaagt gcccggtatg cggggatgga tgcatccagg
     2521 atgcaccggc ggtcctcgaa tcccttgcct ctgtcttctc ccggtgcccc aggtgcacgt
     2581 tcagggtgct cgataaaagc gccccactga agggacggta ttatgggaca ccctgcgaat
     2641 gcgggaagcg gtttatcgac gaggtatttg cccatctcta cgtgatcctg gtggaggagg
     2701 gaattttttc cgggagcgag ccgctcaggg cggtgggatc ccctcttctc caccctggct
     2761 tctttattgc gggccccccg ttcctccccc cgggatccct cgtcctcctc tcgccccttg
     2821 tgacggaatc cgctgcacgg cgcatgatta cagaggtgcc ggaggttcgc ggcgtggtga
     2881 ggtctggcga ctttgttcct ggggccaccg atccttcact tgccactgtt ccgcgtagtt
     2941 acgaacttct tgcaggttgc gatgtgcggg cgggtatctt tcccacgggg gcaggccccc
     3001 tggtcatata tacccagcag tcccagatgc acatcgagtt tccgcgcctg gagaacccca
     3061 agatccagtc cgtggaacgc cacattgccg cacagcgccc cacgtggtgc gtggatgcct
     3121 gctcgggggc ggggacactc gggctctgcg ctgcaaggca tggaataccc ggggtgattc
     3181 tcaacgatgc ctggtattca gccgcgttct ggtccgcgac aaacctccac gtcaaccgga
     3241 tgttcttcca ggtcgaaggg gtacggatac acctgtcctt ccgggagata agcatgcatc
     3301 ccgtggggag ggatccggtc aaggtcgcgg aggcatcggg agggcagtca atcgctgtgt
     3361 accacggtga tttccggcaa ctctggaagg tcctgccacg tgaacctgtc tgtgcggttc
     3421 tcgatctctt cgagaaatcg gacacccgga tgaccgaagc agcaatgaag gaatggcaga
     3481 gccgcgttcc cggcgaggta tttatcccct agggtcgcga tatgttatgg ggactagccc
     3541 gggtggcccg gcgtcacctg tgacccgaaa ccgtcggcat gcggggggcg aagttcggag
     3601 aaggcggtaa cgggctgtct gtgaacggtg caggccaacg ttgaaacctc gtcccatgag
     3661 gtcggtggac agggatcaaa gccccggagg gggcggcgac ctgttgtcgc ggggaccggt
     3721 tcaggcccgg aagggagcag acttaccgtg aacattcggc gcccatgggg tcgcggggcg
     3781 gaggaggaat gcgcctggaa gaggcagccg cgagccgtcg accgagaacg tgtccccatt
     3841 tcgccttggc ggtagcctta tgtcaaaagt caccattgtt ggggccaccg gaaacgttgg
     3901 cactttttct gcccataccc ttgcaggcat tccctatgtg cacgagatcc tcctccacgg
     3961 gagggccggg cgcgaggacc tgctctgcgg gatcacccag gaccttatag attcttttgc
     4021 cgcatcaggc caggcagtgg acctctcctg gacaacggac atcatggatg ccagggattc
     4081 cgacatagtg gtatgcacgg caggagtccc aaggaggccg gggcaggacc gccttgacct
     4141 tgccctccag aacgcccgga tagtggccga tacggccagg gttattggaa aatgtgcacc
     4201 gcacgccatg atcctgatgg tcacaaaccc agtcgacgta atgacatcgg tagccttgag
     4261 atactcggga atgcagccaa accaggtctt cggactcggg acccaccttg attccatgcg
     4321 cctgaaatca ctcatcgcca agcacttcca cgtccacgta agcgaggtgc acacccgtat
     4381 catcggcgag catggggatt caatggtacc tctctggtct gccaccacga tcggagggat
     4441 ccagatcagc aaccttccgg cattctcgcg gcttcccgtt gaagagatgg tagcgactgt
     4501 caaatcgagc ggcgagcaga tcatacggaa caagggagca acggtatacg ggccaggcga
     4561 agcgattgcc accctggtgc ggacggtgct tgggaacgag gaccgcatac tcacggtgtc
     4621 tgcatatatc cggagcgagg tgcacgacat aggggacgtg tgcatcggtg tccctgcaag
     4681 gatcaaccgc agcggcgtct ttcccgtgcc tatcaagatt gccgaggacg aggtggcgca
     4741 gtttggggta tcagtggata aaatacgtgc cgtcacgcac gaaatactgc aaaaacttcg
     4801 ggaataaaaa gtattcctcc ctctcccacg cgcatgcatg ggtggaggcg ggactccttc
     4861 aggtaagggt cagttctaca atctcagcgc gctcgacacc ggagatcttc tggatctcgg
     4921 cttccaccgc gtcgctctcc ccgccggcat cgttcacgat tgccgccacc ttgagcgctt
     4981 ttaagccaaa cccgatgggc tcttcgcgga tatcccgaac ggatggaacc ttctcctgta
     5041 tctccctctt caggaccgca aggtccactt ccggggactc gggcattacc ttgaggataa
     5101 tagccaccgt gcccatggat cagggccccc cgaatccgca tttcgggcac gtgtagggga
     5161 tgctctgctc gcggcaccgg tagcatctcc tgatctgata atcgcacttc gggcacccga
     5221 attcggcggc gccctcttcg gccaggggtg aattgcatga tgtgcacttt cttatcggca
     5281 tggaaccctc ctgatagaat gttctcatat tatctctcct gaaacactta aaagccgatg
     5341 gttgtaccga tggtgggaag tcccgtgagt gcagcgcgta accggtcggg gtgcctgccg
     5401 gagatgacca gtgcatgcat ccggcggcgc aacacgaaag gaaggaagca ggagtcgaca
     5461 tcatccgttt ctacggggga ccggatgcgg gggcagaggc gtccttccac gcggataccg
     5521 tccactgatt tgagcagaag gaggggaagg tgcagggtgt gggcacacca cgctgcaatg
     5581 gtatccgagg tcacctccca gcggtggggg aggggatcta ttgccctgat caggccataa
     5641 gggagcagga tcgcgggccc gcccgggata tcaagcctgg tggtgactgg aaggccaaag
     5701 gaagagaggt accacccgta ctgctccatc gctgagacgg ccatccagtg ggctgcggtt
     5761 ccttcaagcc ggcactctct caccaggtct gcgaatgctc ccccgccggg aatgacaagg
     5821 acgggaacgt ccaggtccac aagaagggcg catatcgacc cggcgcagtc ctggaggctc
     5881 ccgccgatct tcagcaccag tggggggcga accggtgtca ttcctaaaag agagatgcat
     5941 ttcagcaggt taatacctcc ctgccttccc acctttctgg cgtgatccgg accacgatca
     6001 tgctgctgct ggtcctcgca gccccggtga caggcattga gcttgtggaa ttctgccctg
     6061 atccatacct gccggatgac cgtgacgagt acctctgcct cgaagggtgg gggcaggccg
     6121 acggcatcct ggtgacagat ggcgagggag ggatccgttt tcctcccggg accacacttc
     6181 gaggcctgac ggtggtggcc atggacgcag aggcttaccg caacagccac ggggagtatc
     6241 cggatttcga atgggacggg agttctccgg tggtgccgga catgatacgg acggggcggt
     6301 tccagctctc caacactgcc gacgagatcg aggtattttc ggggagggta tgcatccaga
     6361 aggtggattg gccggggaca gtctcgccgc gccaggggca ggtgcactcc ttttccgaag
     6421 gcatgtggga tga
//
LOCUS       DAOQVJ010000022         6428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13286_length_6428_cov_4.838694, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000022 DAOQVJ010000000
VERSION     DAOQVJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13286_length_6428_cov_4.838694"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="PKL26_00925"
     CDS             complement(<1..1833)
                     /locus_tag="PKL26_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82285.1"
                     /translation="MISVLLVDDEPALLDITQIFLEKDGKMSVTLSESAGSALRMLDT
                     ERFDIIVSDYEMPQMNGIDFLKAVKARGVDIPFIIFTGRGREHVAIEALNIGASFYLQ
                     KGGDPKSQFAELRNMIVQAVQRKKAEEEVRLNQHRLQAMIDLHQMSAAHERDICTYAL
                     ETAISLTGSTMGYIAFLNQEETVLSMYAWSKTALSECRVAEKQVIYPVEKTGLWGEPV
                     RQRRPVITNDYAAPNPAKKGHPEGHVKMIRHLGVPVMDGKRIVMVAGFGNKRSEYNED
                     DIRQVTHLMGGLWQILKRKRIEAELVESEKKFRSYFDLPLVGIAITSPDMQWINANQK
                     LCHMLGYSLDELKALKWSDLTPPEELAKELELYTRVVSGESESHTVKKRYIRKDGRLI
                     DVEVSSLPVRKENGELDYFVALIHDISPLMQAKEELEASNNQMAATLEELRATQDSMN
                     DYCRRLEQEEAALRRSEEKFRTMVETAPSMLIITDLSGRITYASPNVRDFTGYLPEEF
                     EGQALSLVHPEDRERVKDDISTAFRELRGVRNLEFRSQKKGGEVWYASSSLEPLRDPQ
                     GAIQGFLIQTIDITSRKKAEEALLKSEEKYRELVESINDIVFSLD"
     gene            complement(1945..2709)
                     /locus_tag="PKL26_00930"
     CDS             complement(1945..2709)
                     /locus_tag="PKL26_00930"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNS82286.1"
                     /translation="MRVILKTKPRAKPRIPIEAEEIIPQNFLRKNDIKIFEGNKERSL
                     DDLFFVTREGPAVSPDQVEVILTGDTGVIKRVGEYMNAGRIIIEGDIGMHCGNFMSAG
                     EILIRGNADAWLGREMRGGRISCEGNAGHYCAAGYRGEKRGMRGGTVEVKGNAGDFCA
                     ENLSGGEVIVHGNCGDMPGVEMRGGTLVIGGDCLRPCGNMTGGTCMVFGQARQFLPTF
                     RRNGSQEAEYAGKRYRMNVFRGDVANRGKGTLMVRG"
     gene            complement(2706..4409)
                     /locus_tag="PKL26_00935"
     CDS             complement(2706..4409)
                     /locus_tag="PKL26_00935"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HNS82287.1"
                     /translation="MTPGLLIKNAFVIDPLSGVKGEIMDIAISNGRIVDEAPRDAEVI
                     DAAGCLTLPGGIDSHSHVCGTKVNFGRYMSPEDMRAGRTARKGVMHVTSGYSVPTTFG
                     NSYRYSTMGYTTVLEGAMAPLEARHTHEEFTATPHQDMLANTLFDGNWSIMEAVREKD
                     VKRAAAIVAWTLAAVRGFAIKLTNPGGTEAWGWGEDVHSIQDLVPHFEVTPAEIIGTM
                     IRANEMLNLPHSVHLHCNNLGVPGNFRTTLDTIDLVPDLNKERQSLYLTHVQFHSYGG
                     STWRDICSRSEDIARAVNGKPQVVMDMGQIMFGRTTTMTADGPMEFKLYMLHHNKWSN
                     HDVELETGSGIIPVYYGRKSLVNCVMWSIGLELALLAKNPWQCLLSTDSPNGAPFVKY
                     PEIIALLMSQRLRESERKMIDPRTEHFVPLFAIERELDWYEIAVMTRAAQAKALGITR
                     IGKGYLAPGAEADVAIYPISPDQVDPSVDYQQVMAAFSRSRFTIKRGRVVARDGEVIV
                     EGANSTIWSKAHVPPEYDMGTDPEFTKKFEQYYTVRMRNYPVQDVYLPRGLRIDTEAS
                     L"
     gene            complement(4406..>4585)
                     /locus_tag="PKL26_00940"
     CDS             complement(4406..>4585)
                     /locus_tag="PKL26_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNS82288.1"
                     /translation="TTHLSRIQIPVAVAGIDAEGTAYRMDGVPIRTRKLFQGRYPDDL
                     EVLEKIYAIVKEAKG"
     assembly_gap    4586..4685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4686..5847)
                     /locus_tag="PKL26_00945"
     CDS             complement(<4686..5847)
                     /locus_tag="PKL26_00945"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNS82289.1"
                     /translation="MRIEDVVCSFCGCLCDDLVVEVEGTRVINVENGCTLANEKFMGD
                     HRLEAPIRRSGGKWEKVSYEEAIAETAGILLDADRPLLYGWSGTYGEAQTIGVHLAEV
                     LGAVIDNTSTVCHGPSIMAIQEVGHPGCTLGQVKNRADVVVYWGANPVDAHPRHMSRY
                     TTYCDGFFVKNAFRERKVVVVDVRETESSQVADEFVKVNPGGDYAVLSALRAIVRGKR
                     DIIPPDVEGVSREQLFRVADMCMAAKFGAFFFGVGLTMAPGKYKNIRNAIELTEELNR
                     HTKFTISPMRGHWNVYGSNEVFTWITGYPYAVDFARGIAFYNPGETTSVDILRRGECD
                     ACLVVASDPGAHFPRACAEHLARIPTVQIDPHVNVTTHLSRIQIPVAVAGIDA"
     gene            complement(5837..6226)
                     /locus_tag="PKL26_00950"
     CDS             complement(5837..6226)
                     /locus_tag="PKL26_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNS82290.1"
                     /translation="MKFLFNTGRTIRQGSYVERKNAPSYREEASAIRMNPVDMFEMMV
                     EEGDRVRVKSSTGEVVMKARADPGLVRGRVFCSLGPYANHIVSPETHGTGMPDFKVTE
                     VEIEPTQDPVSSVAMLMGQCGGVPYED"
     gene            complement(6223..>6428)
                     /locus_tag="PKL26_00955"
     CDS             complement(6223..>6428)
                     /locus_tag="PKL26_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS82291.1"
                     /translation="NKLIDTQMSHGGRVSTDDLLLKVNEGKCQVLHVEKCTGCKTCEH
                     HCPNEAIHVARLLEAVHAPEDEL"
BASE COUNT         1123 a         2004 c         1760 g         1441 t
ORIGIN      
        1 atcgagcgag aagacgatgt cattgatgct ctccacgagc tcgcggtatt tctcctcgct
       61 cttcaggagg gcctcctccg cctttttccg cgaggtgata tctatcgtct gtatcaggaa
      121 cccctgaatt gccccctgcg gatcgcggag cggttcgaga gaacttgagg cataccacac
      181 ttcacctcct ttcttctgcg aacggaactc caggttccgt acccccctga gttccctgaa
      241 ggcggtggag atatcgtcct ttacccgttc cctgtcttcg gggtggacga gcgagagcgc
      301 ctgtccttcg aattcttcgg gcaggtaccc cgtgaaatcg cggacgttcg ggcttgcata
      361 cgtgattctg cccgaaagat cggtgatgat gagcatgctt ggcgccgtct ccaccatcgt
      421 cctgaacttc tcctcgctgc ggcgaagcgc tgcctcttcc tgctcgaggc gccggcagta
      481 gtcgttcatc gagtcctggg tggcccggag ctcctcgagc gttgccgcca tctggttatt
      541 ggaggcctcg agctcctctt tcgcctgcat caggggagag atatcgtgta tgagggccac
      601 aaaatagtcc aattccccgt tctctttcct gacagggaga gaagagacct ccacgtcgat
      661 gagccgtccg tctttcctga tataccgttt cttgaccgtg tgtgattcgc tttcaccgga
      721 aacgactcgc gtgtagagct cgagctcctt cgcaagctct tccggagggg tgagatctga
      781 ccacttgagc gctttcaact catcaaggct gtacccgagc atatggcaga gcttctggtt
      841 tgcgttgatc cactgcatgt cgggtgacgt gatggcaatt cccaccagcg gcaggtcaaa
      901 gtaggaccga aacttcttct cgctctccac aagctcggcc tcaatccgct ttcgcttgag
      961 gatctgccac aatcctccca tcaggtgcgt gacctgccgg atatcatctt cgttgtactc
     1021 ggagcgcttg ttcccgaacc ctgcaaccat cacgatcctt ttaccgtcca tcacagggac
     1081 gccgaggtgc cggatcatct tcacgtggcc ttccgggtgc cccttctttg ccgggtttgg
     1141 cgccgcgtag tcgttcgtga tcaccgggcg cctctggcgg accggctccc cccagaggcc
     1201 tgtcttctcg acaggatata tgacctgttt ttctgcaacg cggcactcgg agagcgcggt
     1261 cttcgaccag gcgtacatgg agagcaccgt ctcttcttga ttcagaaacg caatgtagcc
     1321 catcgtgctt cccgtgagag agattgctgt ctccagggca taggtgcaga tatcccgttc
     1381 atgtgcggcc gacatctggt ggagatcgat catcgcctgg agcctgtgct ggtttaagcg
     1441 cacctcctcc tccgccttct ttcgctgcac cgcctgcacg atcatgttgc gaagctcggc
     1501 aaactgggac ttcgggtcac cccctttctg gaggtagaac gaggcgccga tgttcagcgc
     1561 ctcgatggcg acatgctccc tcccgcggcc tgtgaagatg atgaatggga tatcgacgcc
     1621 tcttgccttc actgccttta aaaaatcgat cccgttcatc tgcggcatct cgtaatcaga
     1681 gacaatgatg tcgaaccttt cagtatccag catgcggagg gcggatcccg ccgattccga
     1741 gagggtcaca gacatctttc catctttttc gaggaatatc tgggtgatat ccaggagagc
     1801 tggttcgtcg tcaacgagga gtacggagat catggggtat cacggtggaa ttacaggaga
     1861 tacactaaaa aagatgcatt tccaggagta aaaggatatg ggagggattg tgccgcatgg
     1921 tatctcggac ctcttcagga agtgtcatcc ccggaccatc agggttccct tccccctgtt
     1981 cgcgacatct cccctgaaga cgttcatccg gtatcgcttg cccgcgtact ctgcttcctg
     2041 ggacccgttc ctgcggaatg tcgggaggaa ctgccgggct tgtccaaaga ccatgcacgt
     2101 cccgccggtc atgttcccgc atggccgcag gcagtccccc ccgatgacaa gcgtcccgcc
     2161 ccgcatctcc acgcccggca tgtctccgca gttcccgtgg acgataacct caccgcccga
     2221 gaggttctcg gcgcaaaaat caccggcgtt ccccttcacc tccacggtgc cacctctcat
     2281 gccacgcttc tcccctcggt atccggccgc acagtaatgg ccggcattcc cctcgcagga
     2341 tatccgcccg ccccgcatct cgcgcccgag ccatgcgtcg gcatttcccc ggatgaggat
     2401 ctccccggcg ctcatgaagt tgccgcagtg catcccaata tccccctcga tgataatcct
     2461 cccggcgttc atgtactcgc ccaccctctt gatcacccct gtgtcaccgg tgaggatcac
     2521 ctctacctgg tccggggaga ccgcagggcc ctctctggta acaaagaaga ggtcatcaag
     2581 ggaccgttcc ttgttcccct cgaagatctt tatgtcgttt tttcggagga agttctgggg
     2641 tattatctcc tctgcctcga tggggattct tggtttcgca cggggcttgg tcttcaggat
     2701 gaccctcata gtgacgcctc cgtatcgatc cgaaggcccc tcggcaggta gacgtcctgg
     2761 accgggtaat tgcgcatcct gaccgtatag tactgctcaa acttcttcgt gaactccggg
     2821 tcggtcccca tgtcgtattc gggcgggacg tgtgcctttg accagatggt gctgtttgcc
     2881 ccttccacta tcacctcgcc gtcgcgtgcc actacccggc cgcgctttat ggtgaagcgg
     2941 gagcgggaga acgcggccat cacctgctgg taatcgactg aagggtccac ctggtccgga
     3001 ctgatcgggt agatggcgac atcggcctcg gctcccggcg ccaggtatcc cttcccgata
     3061 cgggtgattc caagcgcctt tgcctgtgcg gcgcgggtca tcactgcaat ctcgtaccaa
     3121 tccagttctc tctcgatagc gaacaggggc acgaaatgct cggtcctcgg gtcgatcatc
     3181 ttccgctcgc tctcgcgcag cctttggctc atgaggagcg cgatgatctc ggggtatttc
     3241 acgaacggtg caccattcgg gctgtcggtg gagagcaggc actgccaggg gttcttcgcg
     3301 aggagtgcaa gctcgagtcc tatggaccac atgacacagt tgacgaggct ctttctcccg
     3361 taatagacgg ggatgatccc ggatccggtc tcgagctcaa cgtcatgatt gctccacttg
     3421 ttatggtgga gcatataaag cttgaactcc atcgggccat ccgcggtcat ggtggtggtc
     3481 ctcccgaaca tgatctgccc catgtccatg accacctgcg gcttgccgtt caccgcacgg
     3541 gcgatgtctt cagaccgtga gcagatgtcc ctccatgtgg accctccata ggagtggaac
     3601 tggacatgcg tgaggtacag gctctggcgt tccttgttca ggtcagggac caggtcgatc
     3661 gtgtcgaggg tggtccggaa gttcccgggc acccccaggt tgttgcagtg gaggtggacc
     3721 gagtgcggca ggttcagcat ctcgtttgcc ctgatcatcg tcccgatgat ctcggcaggc
     3781 gtcacttcga agtgggggac caggtcctgg atgctgtgta catcctcgcc ccagccccac
     3841 gcctcggtgc cccctgggtt cgtcagtttg atggcaaatc ccctgactgc cgcaagcgtc
     3901 catgccacga tggcggccgc cctcttcacg tccttctccc tgaccgcttc catgatggac
     3961 cagttgccat caaagagcgt gttggcaagc atgtcctggt gaggagtcgc ggtgaactcc
     4021 tcgtgggtat gcctcgcctc aagaggtgcc atcgccccct cgaggactgt cgtgtacccc
     4081 atggtgctgt aacggtagga gttgccgaac gtcgtgggca cgctgtaacc ggatgtcacg
     4141 tgcatgacgc ccttccgcgc ggtcctccct gcccgcatgt cctccgggct catgtacctg
     4201 ccgaagttca ccttggtgcc gcagacgtgg gaatgggagt cgatcccgcc agggagggtg
     4261 aggcatccgg ctgcgtctat gacctccgcg tctcggggtg cctcgtcgac aatcctcccg
     4321 ttactgatcg cgatatccat gatctctcct ttcacgcccg agagtgggtc gatcacgaac
     4381 gcattcttaa tcaggagccc tggcgtcatc cctttgcctc cttcacgatg gcataaatct
     4441 tctcaagcac ctcaaggtcg tcaggatatc ttccctggaa gagcttccgg gtccggatcg
     4501 ggacgccgtc catccggtag gcagtccctt cggcatcgat acccgcgacc gcgaccggga
     4561 tctgaatgcg cgagaggtgg gtggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnncggca tcgatacccg cgaccgcgac cgggatctga atgcgcgaga ggtgggtggt
     4741 cacgttgaca tgagggtcaa tctggaccgt agggatgcgt gcgaggtgct ctgcgcatgc
     4801 ccgcgggaag tgtgcgccag gatcgcttgc gacgacaagg caggcatcgc actctcccct
     4861 cctgaggatg tccacactcg tggtctcccc gggattgtag aatgcaattc ctctcgcaaa
     4921 atcgaccgca tagggatacc cggtgatcca agtgaatact tcgtttgaac catacacgtt
     4981 ccagtgcccc ctcatcgggg aaatggtgaa cttggtgtgc cggttcagct cctcagtcag
     5041 ctcgatggcg ttccggatat ttttgtactt ccccggagcc atcgtgagtc ccacgccaaa
     5101 gaagaatgcc ccgaactttg ctgccatgca catatccgca accctgaaga gctgctcgcg
     5161 ggagaccccc tcgacatcgg gcgggatgat atcgcgcttg cccctcacga tcgcccggag
     5221 tgccgagagg accgcgtagt cgcccccggg gtttaccttc acaaactcgt ctgcgacctg
     5281 agaggactcg gtctctcgga cgtccacgac caccaccttc ctctccctga atgcgttctt
     5341 cacaaagaac ccgtcgcaat aggtggtgta cctgctcatg tgcctgggat gtgcgtcgac
     5401 cgggtttgca ccccagtata ccaccacgtc cgcccggttc ttgacctgcc cgagagtgca
     5461 gccggggtgg ccgacctcct ggatcgccat gatggacggg ccgtggcata ccgtcgaggt
     5521 gttgtcgatg accgcgccga ggacctctgc caggtgaacc ccgatggtct gggcctctcc
     5581 gtacgtcccg ctccatccgt agaggagcgg acggtcggcg tcaaggagga tgccggcggt
     5641 ctccgcgatc gcctcctcgt aggagacctt ctcccacttc ccccctgacc ttcttatcgg
     5701 ggcctccagc cggtggtccc ccatgaactt ctcattcgcg agcgtgcagc cattttcgac
     5761 gttgataacg cgggttccct caacctctac gacgaggtcg tcgcacaggc acccgcaaaa
     5821 cgagcagacc acgtcctcaa tcctcatacg ggacaccccc gcactgcccc atcagcatcg
     5881 cgaccgagga taccgggtct tgcgtcggct cgatctccac ctcggtgacc ttgaaatccg
     5941 gcatcccggt gccgtgggtc tccgggctga cgatgtggtt ggcgtacggc ccgagactgc
     6001 aaaagaccct cccacggaca agtccgggat cggctcttgc cttcatcacg acctctccgg
     6061 tgctgctctt caccctcacg cggtcgccct cctccaccat catctcgaac atgtccacgg
     6121 ggttcatgcg gatcgctgat gcctcttccc ggtacgaagg ggcgttcttt cgctcaacat
     6181 acgatccctg ccggatcgtc ctgcccgtgt tgaagaggaa cttcatagtt cgtcctccgg
     6241 cgcatgaacc gcctcgagga ggcgggcaac gtggatcgct tcgttcgggc agtggtgctc
     6301 gcaggtcttg caccccgtgc acttctccac gtggagcacc tggcacttcc cctcgttcac
     6361 cttaaggagg aggtcatcgg tggatacgcg accaccgtga gacatctgtg tatcgatcag
     6421 cttgttca
//
LOCUS       DAOQVJ010000023         6347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13523_length_6347_cov_4.125238, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000023 DAOQVJ010000000
VERSION     DAOQVJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13523_length_6347_cov_4.125238"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKL26_00960"
     CDS             complement(<1..910)
                     /locus_tag="PKL26_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HNS82292.1"
                     /translation="MEIRKVQITGGSSFVITLPKEWAEKQNVKKNDPLGLVTQPDGTL
                     LVTRDLSESPAQRAKTFDVTSIKDPTFLFRLLIGSYIAGYTSIAITSKQRIPASIRMV
                     VREFTQMTIGPEVVEETDTSVLLKDLLNPAEMPFENTIKRMYVIVKNMYMDALAVLES
                     GNGTLAHDVVARDNDVDRLHWLLARQTNIILKNASLARKMGVAPSVVVNTYIISRIIE
                     RIGDHAVRYVEQAMKVPGGGLDPETMETIRTASAHSLSIFDRSIVSFFKADIRESHTN
                     IEQVEHLERLCQEITSRSMEHPPEVAV"
     gene            1030..1899
                     /locus_tag="PKL26_00965"
     CDS             1030..1899
                     /locus_tag="PKL26_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS82293.1"
                     /translation="MPDSRTQVLFATSLALILAIALVAAGCTQPQGGSPKAPEQKIQS
                     LSIAGSTTVLPVAQAAADAFMNTRGDADIRVSGGGSSVGIQSAGERTADIGMSSRDLK
                     PEEKARYPDLVATVIGNDGIAVIVHSGNSVGPLTLEQVKGVYQGTYTNWKELGGPDLA
                     IVVVGRDSASGTREFFHEKVMKKEDFVPTQLEKNSNGAVKQTVMQTPGAIGYVGLGYV
                     DATTKAVPVMVSGSPVDPTVGNVLDGKYPIARPLLMVTMGEPGGLAKEYLAFLTGPSG
                     QAILAEEGFVPLV"
     gene            1945..3906
                     /gene="pstA"
                     /locus_tag="PKL26_00970"
     CDS             1945..3906
                     /gene="pstA"
                     /locus_tag="PKL26_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNS82294.1"
                     /translation="MNEPRQSTGIFCHGRQARNKESLMGLLWFSCAIFSVAVIFFILA
                     FLFYDGFPIFVQAGLPAMLTGLVWNPTGTPPLYGTLPLIAGTLLVMLGAMAMAVPLGI
                     GSAIYIAELAHPRVKAVAKPAIELLAGIPSVVYGFFGLIVLTDWIRVTFGVPTGESWL
                     AGSILLGIMSLPTIISVSEDAIASVPREFREGSLALGATRWQTISRVVVPSAVSGITA
                     AVILGMGRAIGETMAVLMVTGNAAIIPDPIWNVLSPVRTLTGTLGIEMGEVAIGSAHY
                     HALFGVAVVLLAITLMVNLAATFILARIRQQNAPAARSCRTSRLLLNDMGSRFAWHGS
                     LKPFLLVGVISLAGLPVAGVAGFFAVALALLVLWRLKAKVSPKLAQKVAFFLLLAATA
                     TVIAILAIILFDIVSKGLPGLTWEFLTEPPRNLGRAGGIFPALVGTLYLVAGAIAIAL
                     PLGIGAAIYLTEYTREGKLTRAIRTGTDLLNGTPSIVFGLFGFSFLVLSLGFGVSLIA
                     GQVTLALMVLPTIIRTTEEALKNVPHALREGSLALGATRWQTISRVVIPPAVPGIITG
                     AILSIGRAAGETAPILFTAAVFSQRFLPSSLFDPVMALPYHLYILATNVPGADANKYA
                     TALVLLILVVLIYSAAILVRTHSQKTTRG"
     gene            3912..4673
                     /gene="pstB"
                     /locus_tag="PKL26_00975"
     CDS             3912..4673
                     /gene="pstB"
                     /locus_tag="PKL26_00975"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNS82295.1"
                     /translation="MTTQSIISTQGLTLWYGETRALKGVTMEVADQKVTALIGPSGCG
                     KSTLIRCFNRMNDLVCDCRIEGSVDFHGNNIYGNGADPVDIRRRIGMVFQKPNPFPKS
                     IYENVAYGPRVHGVTDRARLDQVVEESLRKAALWDEVRDRLSHSAMCLSGGQQQRLCI
                     ARTLAVGPEVILMDEPCSALDPIATAKIEALIGELKQQYCVIIVTHNMQQASRVSDFT
                     GFMYLGEMVEFGETAQIFESPREELTENYITGRFG"
     gene            4685..5341
                     /gene="phoU"
                     /locus_tag="PKL26_00980"
     CDS             4685..5341
                     /gene="phoU"
                     /locus_tag="PKL26_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNS82296.1"
                     /translation="MTDKFHDEMAGMQQKVLGMGLFAEGMLRDAVEALRTQDISLAGD
                     VKARKGALSSQNDILEEEVYRMIALYQPVARDMRAVACALKVIASSERIGRYGKDIAN
                     VVIATADSRGKDLASGLAIPHMAGLVLSMIDDSLRAYETGDLEFIRHHSERDDVVDAL
                     RHTIFREGITYMMEEPRTITRCMNYLMVARYLERCADHSCKIAEKVHYMVTGERIEIK
                     "
     gene            5625..>6347
                     /locus_tag="PKL26_00985"
     CDS             5625..>6347
                     /locus_tag="PKL26_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HNS82297.1"
                     /translation="MPQGQLVSEPLLPGESLSSVQVQARRPILRAKSGPALSRESIHT
                     VERDGISYLVSENYSYKSEAYYAILMQETQGLRVEPGTKAILDAFVVPICLERARMDG
                     IPVADYSISQSCIPAPAVIYGLNYFACTSHFEVLGQDDSARELVRHVTNNGKYPFCSQ
                     RLDAGARIERAVSIFGNVSCTDPAWAEMAAGVWESFRIPLVEIVCIRGPGGLRLSSIC
                     PVRYSRLTPAEKELLSLYLSGQV"
BASE COUNT         1188 a         2033 c         1883 g         1243 t
ORIGIN      
        1 gcactgcaac ctcaggcgga tgctccatcg agcgcgaggt gatctcctgg cagagccgct
       61 caaggtgctc cacctgctcg atattggtat gcgattcccg gatgtcagcc ttgaagaacg
      121 agacgatgct ccgatcgaaa atggagaggg agtgggcgga ggccgttcgt atcgtctcca
      181 tcgtttcagg gtcgagtccg cctcctggca ctttcatcgc ctgctcgacg tacctgaccg
      241 cgtggtcccc tatccgctcg atgatcctgc tgattatata cgtgttcacg acgacggacg
      301 gcgccactcc catcttcctg gcgaggcttg cattcttgag gatgatgttc gtctgccggg
      361 ccaagagcca gtgaaggcgg tctacgtcgt tgtcccttgc caccacgtcg tgcgcgaggg
      421 tgccgttgcc agactcgagc actgccaggg cgtccatgta catgttcttg acgatcacgt
      481 acatgcgctt gatggtgttc tcaaatggca tctcagccgg gttgagcagg tctttcaaga
      541 ggacggaggt atcggtctcc tccaccacct cgggcccaat tgtcatctgg gtgaactccc
      601 tcaccaccat ccggatcgag gcaggaatgc gctgcttcga ggtgatggca atcgacgtgt
      661 agcctgcaat gtaggacccg atcaggaggc ggaagaggaa cgtggggtcc ttgatggacg
      721 tgacgtcaaa ggtcttggca cgctgtgccg gggactcgga gaggtcccgc gtcacgagca
      781 gcgtgccgtc aggctgggtc acgaggccaa gcgggtcgtt cttcttcacg ttctgcttct
      841 ctgcccattc ctttggcaga gtgatcacaa acgacgaccc tcccgttatc tgcaccttcc
      901 ggatctccac tcctctcctc ctgccctttt tctatactac tctattcccc acaatatatt
      961 atatatatac atatagtctc catcgaaaaa aggtataccg gaaccccccg aattttttct
     1021 tcgaacacca tgcctgattc acgcacacag gtgctttttg ccaccagcct cgcgttgatc
     1081 ctcgcgattg cgcttgtcgc ggccggctgc acgcagccgc agggggggtc gccgaaggcc
     1141 cctgaacaga agatccagtc cctctccatt gcgggttcta ccaccgtgct cccggttgcc
     1201 caggctgcgg ccgacgcgtt catgaacacc cgcggcgacg cggacatccg ggtcagcggg
     1261 ggcggctcca gtgtcggcat ccagtccgca ggcgagcgta ccgcggacat cggcatgtcc
     1321 tcgcgcgacc tgaagcctga agagaaggcc aggtacccgg acctcgttgc cactgtgatc
     1381 ggaaacgacg gcatcgcggt gatcgtccac tccggcaact ccgtcgggcc actcaccctg
     1441 gaacaggtga agggagtgta ccaggggacc tacaccaact ggaaggaact cggcggacct
     1501 gaccttgcga tcgtggtggt ggggagggac agcgcctcag ggacaaggga gttcttccat
     1561 gaaaaagtga tgaagaagga ggacttcgtt cccacgcaac tcgagaagaa ctcgaacggt
     1621 gccgtgaagc agacggtcat gcagacgccc ggggcaatcg ggtatgtcgg gctcggctac
     1681 gtggacgcga ccacaaaagc ggtcccggtc atggtctccg gatccccggt cgatccgact
     1741 gttgggaacg tgctcgacgg aaagtatccg atcgcaaggc ctctcctgat ggtcacgatg
     1801 ggagaacctg ggggactcgc aaaggaatac cttgcattcc tgacagggcc ttcagggcag
     1861 gcgatcctcg cagaagaggg gttcgtgccc ctggtatagt cccccggcat cccacatgtc
     1921 cttcctgacc gagaccagaa tcccatgaat gaacccaggc agtccaccgg gatcttctgc
     1981 cacgggaggc aggcacgaaa caaggagtcc ctcatggggc tcctctggtt ctcctgcgca
     2041 atcttctcag tcgccgtcat cttcttcatc ctcgcgttcc tgttctacga cgggttcccc
     2101 atcttcgtcc aggcaggttt gcccgcgatg ctcaccggcc tcgtgtggaa ccctaccggg
     2161 acccctcccc tgtacgggac cctccccctg atcgccggca ccctgctcgt catgctcggt
     2221 gcaatggcaa tggcagtccc gctcggcatc gggagtgcga tctacatcgc ggagctcgcg
     2281 catccccgcg tgaaagccgt cgcaaagcct gccatcgagc tcctggcagg gatcccttcc
     2341 gtggtgtacg ggttcttcgg gctgattgtc ctcaccgact ggatacgggt caccttcggc
     2401 gttcccaccg gcgagtcgtg gctcgcgggg tcaatactcc tcggcatcat gtcgcttccc
     2461 accatcatca gcgtctcgga ggacgccatc gcctcggtcc cccgcgagtt ccgtgagggc
     2521 agcctcgcgc tcggggccac gagatggcag accatctccc gggtcgtcgt cccctccgcg
     2581 gtctcgggaa ttacagccgc ggtcatcctc ggcatgggga gggcgatcgg ggagaccatg
     2641 gcggttctga tggtgacagg gaacgccgcc atcatccccg acccgatctg gaatgtcctc
     2701 tccccggtcc ggaccctcac cggcaccctc gggatcgaga tgggtgaggt cgcaatcggg
     2761 agcgcgcact accacgcgct cttcggggtc gcggtggtgt tgctcgcaat caccctcatg
     2821 gtgaacctcg ccgcgacctt catcctcgcg aggattcggc agcagaacgc accggctgca
     2881 aggtcctgca ggacatcccg gctcctgctg aatgatatgg gatcgcggtt cgcatggcac
     2941 gggtccctca aaccattcct cctggtaggt gtcatctcgc ttgcggggct ccccgtcgcg
     3001 ggcgtcgcgg gcttttttgc cgttgcgctc gcactcctcg ttctctggcg cctgaaggcg
     3061 aaagtctccc cgaaacttgc gcagaaggtc gcctttttcc ttctccttgc cgccacggcg
     3121 acggtgattg cgattctcgc catcatcctc ttcgatatcg tcagcaaggg ccttccgggc
     3181 ctgacatggg agttcctcac agagcccccg aggaacctcg ggcgtgccgg cggcatcttc
     3241 cctgcactgg tgggaaccct ctacctcgtg gcaggggcga tcgcgattgc gctcccgctt
     3301 gggatcggcg cggccatcta cctgaccgag tatacccggg aggggaaact cacgcgggcg
     3361 atccgcacgg ggaccgacct cctgaacggc accccttcca tcgtctttgg gctcttcggg
     3421 ttctcgttcc tggtcctctc cctcggattc ggggtctcac tgattgcagg acaggtgacg
     3481 cttgcgctga tggtgcttcc gaccatcatc cggaccacgg aggaggcctt gaaaaacgtt
     3541 ccccacgcgc tcagggaggg aagccttgcg ctgggggcaa cgaggtggca gacgatctcg
     3601 cgggtggtga tcccccccgc agtgcccggg attatcaccg gcgccatcct ctccatcggg
     3661 agggctgcgg gcgagaccgc ccccatcctc ttcacggcgg cggtcttctc ccagaggttc
     3721 ctcccctcct cgctctttga ccccgtcatg gcgctcccct accacctcta catcctcgcc
     3781 acgaacgttc ccggcgcgga tgcgaacaag tatgcgactg cactggtgct cctgatcctg
     3841 gtggtcctga tctactcggc ggccatcctg gtacggaccc attcccaaaa gaccacacga
     3901 gggtaatacc attgacaacc cagtccatca tctcaacgca gggcctcacc ctgtggtacg
     3961 gagagacacg cgccctgaaa ggcgtcacga tggaggtagc ggaccagaag gtgactgccc
     4021 tcatcggccc gtcagggtgc ggcaagtcca ccctgatccg ctgcttcaac cgcatgaacg
     4081 acctcgtttg cgactgccgg atagaggggt cggtcgactt ccacgggaat aacatctacg
     4141 ggaacggcgc agaccccgtc gatatccgcc gccggatcgg gatggtcttc cagaaaccga
     4201 atcccttccc gaagtccatc tacgagaacg tggcctacgg gccgagggtc cacggcgtca
     4261 ccgatcgggc gcggctcgac caggtcgtcg aggagagcct gcggaaggcc gcgctctggg
     4321 acgaggtgag ggaccggctc tcccactcgg cgatgtgcct ctcgggcggc cagcagcagc
     4381 ggctctgcat cgcaaggacg ctcgcggtcg ggcccgaggt catcctgatg gacgagccgt
     4441 gctccgcgct cgacccgatc gcgacggcaa agatagaggc gctcatcgga gaattgaagc
     4501 aacagtactg cgtgatcatc gtgacgcaca acatgcagca ggcatcgcgg gtgagcgatt
     4561 tcaccgggtt catgtacctc ggggagatgg tcgagttcgg ggagaccgcc cagatattcg
     4621 aaagcccaag ggaagaactg acagaaaact acattacagg gaggtttggg taggtggtgt
     4681 gcacatgacc gacaagttcc acgacgagat ggctggaatg cagcagaagg tcctcgggat
     4741 ggggctcttc gccgaaggga tgctccgcga cgcggtggag gcactgagga cacaggacat
     4801 ctcacttgca ggcgatgtca aagcccggaa aggggcactc tcctcgcaga acgatatcct
     4861 cgaggaggaa gtttatcgga tgatcgcgct ctaccagccg gtggcaaggg acatgcgggc
     4921 cgtcgcatgc gccctgaaag tcatcgcctc ttcagaacgg atcggccgct atggaaaaga
     4981 catcgcaaat gtcgtcatcg ccaccgccga ctcccgcggg aaagacctgg caagcggcct
     5041 tgcaatcccc cacatggcag gcctcgtcct ctcgatgata gacgattccc tgcgggcata
     5101 cgagaccggc gacctggaat tcatccgcca ccactctgaa cgcgatgacg tggtggacgc
     5161 gctccgccat accatcttcc gcgaggggat cacttacatg atggaggagc cccgcaccat
     5221 cacccgatgc atgaactacc tgatggtcgc ccgttacctg gagcggtgcg cagaccactc
     5281 ctgcaagatc gccgagaagg tccattatat ggtgacggga gaacgaatag agataaagtg
     5341 atgcgtgtgg tgcagcgctc ccggcgcgcc tgcgaatcgg gaccttctgc ttcatttttt
     5401 cacgcacatc gcggcagtcc gaccacaaac atttaaacca agcctgccac accatctgag
     5461 taatacctct tgtgctgcag accctgcggc tcgtggttcc ccatgccaga accagagaac
     5521 gagaacgacg atgaccctca tgatagggtc cacactgccg gtttttcgaa cacgtcccgc
     5581 tgtcccgggc gcgatattcc cctccacgcg gaggtgctca cctgatgccc cagggacagc
     5641 tggtcagcga gccgctcctc ccgggtgagt ccctctcctc cgtacaggtc caggcacgaa
     5701 ggcccatcct gcgggcaaag agcggcccgg cactttcccg ggagagcatc cacacggtgg
     5761 agagggatgg gatatcctac ctcgtctccg agaactactc ctacaagagc gaggcgtatt
     5821 acgcgatcct catgcaggag acgcaaggcc tgcgggtgga gccgggcacg aaggcgatcc
     5881 tcgatgcatt cgtcgtgccc atctgcctcg agcgggcccg gatggacggg atacctgtcg
     5941 ccgattactc gatatcccag tcgtgcatcc ctgcgcccgc ggtcatctac ggtctgaact
     6001 actttgcatg cacctcgcac ttcgaggtgc tgggccagga cgattcggcc cgcgagctgg
     6061 tcaggcacgt gaccaacaac gggaagtacc ccttctgctc gcagcgcctt gatgcgggcg
     6121 cacggatcga aagggccgtc tccatcttcg ggaacgtttc ctgcacagat ccggcgtggg
     6181 cagagatggc tgcaggggtg tgggagtcct tccggatccc gcttgtcgag atcgtctgca
     6241 tcaggggtcc gggcggcctt cggctctcct cgatctgccc tgtccgctac tcgaggctga
     6301 cgcccgctga aaaggagctc ctctctctct acctctcggg ccaggtc
//
LOCUS       DAOQVJ010000024         6256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13805_length_6256_cov_4.328979, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000024 DAOQVJ010000000
VERSION     DAOQVJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13805_length_6256_cov_4.328979"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PKL26_00990"
     CDS             complement(<1..1018)
                     /locus_tag="PKL26_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNS82298.1"
                     /translation="MPEKVYFTGVRAREPGQNTISKIQRLFDAAGFPRMIEPGDLTAI
                     KLHFGEWGNESYISPVHVRQVVDRVKDVGGKPFLTDSNTLYRGRRSNAVDHLCTAVEH
                     GFAYAVVGAPLIIADGLRGGNWRNVDLDSRHFPAVRVSGDIADSQAMIVLSHFKGHDV
                     AGFGGAIKNLAMGCAPPAGKQAQHCARPHHIVERCTGCGKCVPVCPGEAISVVETGNG
                     KIARIEHARCVGCFECMSACPSNAIDFDWETEIPEFIERMVEYAFGVVKSRQKKTGYM
                     NFLLRITPDCDCVPWSDVSIVPDIGILASRDPVAIDAASYDLVNAEEGNRGSFLQKNH
                     GKGKD"
     gene            complement(1173..1661)
                     /locus_tag="PKL26_00995"
     CDS             complement(1173..1661)
                     /locus_tag="PKL26_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNS82299.1"
                     /translation="MLKKSMEEALNKQVNAELYSSYLYLSMAAYCETQLMKGFAKWLR
                     LQADEEREHAMKFFDYIIEAGGTVKLAKIEAPKTGWKSIGEVFDEVYEHEMKVTGLIG
                     GLMDLAIKEKDYATRNFLGWFVKEQVEEEAHASEIQGKIKMLGDVVGHLFWLDHELGK
                     RE"
     gene            complement(1868..2608)
                     /locus_tag="PKL26_01000"
     CDS             complement(1868..2608)
                     /locus_tag="PKL26_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82300.1"
                     /translation="MSKYSWQIKLGIVLLASSFFIYSLKFLFLGDPENTYYYVFNALG
                     FLPINVLLVTLVLNELLTVRARKERLEKLNMVIGTFFSEAGTDLLKTIARADHTISTL
                     EDTLRVTGDWKDEDFLRVRKVMEGYDFHVDITRIDLGNMREYLKERRNFLLRLLENPM
                     LLEHQTFTEVLRAVFHFTEEIERRDALACMTERDCMHLKGDVERIYRLLSLEWLAYMK
                     HLHAHYPYLSSLAMRTNPFDSKASVVIG"
     gene            complement(2671..3798)
                     /locus_tag="PKL26_01005"
     CDS             complement(2671..3798)
                     /locus_tag="PKL26_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82301.1"
                     /translation="MDNSGGAVPPAGVAGGDFMQWQEDDLAARLPAMVKGRQDAGLEG
                     LVGGLEAVVINTEPGRLEPGVDELLHFTGLGIAERFVDSNATAYVLATRDSPSIVIMS
                     RGRGANPFHPFNQAEKARALPNTRLETFIFRARDLDTYVGIQRELGVRFLTERIVDAP
                     EYRFIQTYPSRFTGNSLGFLEWKGSGRSFAGAGAKRLPITQRKPDLPWLSQIHGLDHT
                     ATRVRAVERNSAIIEFLSLTGYHFDFAVFVKSLNSITSVARLSRDDFAMVFTSGIAPY
                     TDDEASGPTEKFIRSYGTRVHHMAFRTDEIEEVFGRLRGSGMDFLVDLVGSPDEGLKQ
                     TFSMPSPYTMVVNEYIHRYEGFDGFFTRSNVEALTRATGRQ"
     gene            complement(3931..4074)
                     /locus_tag="PKL26_01010"
     CDS             complement(3931..4074)
                     /locus_tag="PKL26_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNS82302.1"
                     /translation="MAIDPICKMTVDEKTAKFTSQYKGKTYYFCAPGCKKKFDADPEK
                     YLI"
     gene            complement(4079..>6256)
                     /locus_tag="PKL26_01015"
     CDS             complement(4079..>6256)
                     /locus_tag="PKL26_01015"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS82303.1"
                     /translation="VETIETALSTLPGVVRAQVNLATEKAQVTYNSSLTTLEDMRAAI
                     EDAGYQYLGLADQLTVEAEEEARRRDLDDKFRRFMVGFAVSIPLFALMYIPLPISMHA
                     LSYLMLAVSTPVFIYVAYPIFRAARMAARNRTLNMDVMYAMGTGVAYVSSVLGTLGIV
                     LTHEFMFYDTAIMLASFLILGRYLEARAKGRTSAAIRALMGLRPKTALLVTGEGEKEV
                     PIEDVQVGDRIRVKPGGKVPVDGTVTEGESYVDESMITGEPIPVLKAAGAQVVAGTLN
                     TNSIFTFRAEKVGKDTVLAQIIRLVEDAQASRPPVQRIADVAVSYFIPTVLAIAFAAF
                     LVWYLVIGSTLLFALTALVSVLVVACPCALGLATPTAVTVGVGRGAELGVLVRNGEAL
                     ETADQVNTVVFDKTGTLTRGKPAVTDIFPSGIDQETLLGIAAAVEKNSQHPLADAVVQ
                     RTLLEGAPIGEAEGFDTHGGKGVSARVLGEEVLVGNRAFLEEKGVVLAQDRESVVRGL
                     QNEGKTVILIAVGGNLAGVLGVADTVKETTPSALEVLKGMGMKVAMITGDNRLTATAI
                     GKRLGIEEVFAEVLPGDKAKEVEKLQEAGNIVAFVGDGINDAPALARADVGIAIGSGT
                     DVAIESGDIVLVRDDLLDAAAAIQLSRKVMSRIRQNIFWAFAYNAALIPLAAGIFYPF
                     TGYTLRPELAALAMALSSVTVVTLSLSLKRYIPPAKRYHSLKV"
BASE COUNT         1278 a         1878 c         1688 g         1412 t
ORIGIN      
        1 tgtcctttcc ctttccatgg ttcttttgca gaaacgaccc cctgttcccc tcctctgcat
       61 tcactaggtc gtaacttgcg gcgtcgatgg ctaccgggtc ccgtgaggcg aggatgccga
      121 tgtctgggac gatggagacg tcgctccatg gcacacagtc acagtccgga gtaatacgca
      181 ggaggaagtt catgtacccc gtcttttttt gcctgctctt taccaccccg aatgcatatt
      241 ctaccatcct ctcgatgaat tcaggaattt ccgtctccca gtcgaagtcg atggcattcg
      301 acgggcatgc actcatgcat tcgaaacatc cgacacagcg ggcgtgctcg atgcgggcta
      361 ttttaccatt ccctgtctca acaaccgata tagcctctcc agggcagact ggcacacact
      421 tgccgcaccc ggtacatctt tccacgatgt ggtgggggcg ggcgcagtgc tgtgcctgct
      481 ttcccgccgg gggggcacat cccatggcca ggttctttat cgccccccca aagccggcta
      541 catcatgccc cttgaagtga gagaggacga tcatggcttg cgagtccgca atgtcgcccg
      601 acacccgaac ggcagggaag tgcctggagt cgaggtccac attcctccag ttccccccgc
      661 gaagaccgtc cgcaatgatc agtggggcgc cgacgacggc ataggcaaag ccatgctcca
      721 cagccgtgca gaggtggtcg accgcattgc tgcgcctccc gcggtagagc gtgtttgaat
      781 cggtcagaaa cggttttcct cccacatcct tcactctgtc cacgacctgg cggacatgca
      841 cggggctgat ataggactcg tttccccatt ccccgaagtg caacttgatc gcagtgaggt
      901 ctccgggttc tatcatccgg ggaaacccgg ccgcatcaaa aagtcgctgt atctttgata
      961 tggtgttctg accgggttca cgtgccctca cgccagtaaa atatactttc tcaggcatcg
     1021 cagagttccc ctcctctcat cacaattggg agtgagcatc ttaatagggc tatccactca
     1081 ggatgaatct gaaggcaatt ccaactatgg aaaattcaca cgcattgtga tgcgggaaca
     1141 ccaaaaaaac cttgcaagaa aaagattgag ggttattccc tcttcccgag ttcatggtcc
     1201 agccagaaga gatgcccgac gacatcgccg agcatcttga tcttcccctg gatttcgctt
     1261 gcgtgtgcct cttcttccac ctgctctttc acgaaccagc caaggaaatt ccgggtggca
     1321 taatccttct ccttgatcgc aaggtccatc agcccgccaa tgaggcctgt aaccttcatc
     1381 tcatgctcgt agacttcgtc aaaaacctcg ccgatggact tccaccctgt ctttggtgct
     1441 tcgatttttg ccagtttgac ggtcccgcct gcctcgatga tgtaatcaaa gaatttcatg
     1501 gcatgttccc gctcttcatc tgcctggagc cggagccact tggcaaaccc tttcatcagc
     1561 tgtgtctcgc agtatgcggc catggacagg tagagatacg atgagtagag ctctgcgttc
     1621 acctgcttgt tcagcgcttc ttccatactc ttttttagca acagtattcc tccagaacat
     1681 ggaggtattg accaagacgg gctttaatgg tttggtttat gcctcctgcc tgggaatggc
     1741 acgcggctcc catggcagga gcattaggct gtgtcatgtg cgactctccc gtcccataac
     1801 cggataattt atctcttctg gttattcgcc gctttatccc ggagatctca agaaaagata
     1861 ggtggaatca accaatgact accgaagcct tgctgtcgaa cgggttggtc cgcatcgcca
     1921 gcgatgagag gtaagggtag tgggcatgga ggtgcttcat ataggcgagc cattcgagag
     1981 aaaggagacg gtatattctc tccacgtccc ccttgaggtg catgcagtcc ctctccgtca
     2041 tgcaggcgag agcatccctt ctctcgatct cctcggtgaa atgaaagaca gcccgcaaca
     2101 cttctgtgaa tgtctggtgt tcaaggagca tggggttttc aagaagacgg aggaggaagt
     2161 tcctcctctc tttgagatat tccctcatgt tcccgagatc tatccgcgtg atatccacat
     2221 ggaaatcata tccctccatg acctttcgca ccctgagaaa gtcctcgtcc ttccaatcac
     2281 cggtcacccg aagggtgtcc tcgagggtcg agatcgtatg atccgccctc gctatcgtct
     2341 tgagcagatc tgttcccgcc tcggaaaaga acgttcctat caccatgttc agcttctcca
     2401 ggcgctcctt cctggcccgg acagtcagga gctcgttcaa cacgagggtg accaggagca
     2461 cgttgatggg aaggaacccc agcgcgttga agacataata ataggtgttc tccggatctc
     2521 cgaggaagag aaacttgagc gagtaaatga aaaaagagct tgccaggagg acgatcccaa
     2581 gtttgatctg ccaggaatat ttgctcatgc taccactcat ctctcagcgc ttcgcattct
     2641 ctggagaaat atgtgccgca tgcattggga tcactgtcgc ccggttgccc tcgtcagtgc
     2701 ttccacgttg ctcctggtaa aaaacccgtc aaatccctcg tacctgtgta tgtactcgtt
     2761 gacgaccatc gtatacggtg acggcatgga gaatgtctgc ttgagaccct cgtcagggga
     2821 cccgaccagg tcaacgagga aatccatgcc gctcccgcgg agcctgccaa acacttcttc
     2881 gatctcatca gtgcggaagg ccatgtggtg aacacgggtt ccataggaac ggataaactt
     2941 ctctgtaggc cctgatgctt cgtcgtcagt gtagggtgcg atcccggaag tgaagaccat
     3001 cgcaaagtcg tccctgctca ggcgggcgac gctcgtgatg gaattgaggg acttcacgaa
     3061 cacggcaaaa tcgaaatgat agcccgtgag cgagaggaac tcgatgatgg cgctgttcct
     3121 ctcgaccgcc ctcacccttg tcgcggtatg atcgagcccg tgtatctgcg agagccaggg
     3181 gaggtccggt tttctctggg tgatggggag tctttttgcc cccgctccgg caaacgagcg
     3241 accagatccc ttccattcga gaaaaccaag cgagttcccg gtgaaccggg atgggtacgt
     3301 ctggatgaac cggtactctg gtgcatcgac gattctttca gtcaggaacc ggacacccag
     3361 ttcccgctgg atcccgacat aggtgtcaag gtcccgcgcc cggaagataa aggtctcgag
     3421 gcgggtattg ggaagtgccc tggctttttc agcctggttg aagggatgga aaggattcgc
     3481 gcctcttccc ctgctcatta tgacaatcga cggtgaatcc ctggtcgcga gtacataggc
     3541 agttgcgttg gagtcaacga aacgctcagc gattccaagg cctgtgaagt gcagcagttc
     3601 atccacgccg ggttcaagcc tcccgggctc tgtgttgatg accaccgcct caaggcctcc
     3661 aaccagccct tcgaggcctg catcctgcct gcccttcacc attgcgggca gccttgccgc
     3721 aaggtcatcc tcctgccact gcatgaagtc ccctccggca actcccgccg gcgggactgc
     3781 ccctccggaa ttgtccattc ctgcaccctc ctttattggc agaaggtgcg gaaggttgaa
     3841 tgataaaggt tgggaccgcg gaagcagcag gtggagggaa gggcctccgc cagtccaggg
     3901 tgctgatcct gcagttgcgc tcccatcccg ctatatcagg tatttctctg gatctgcatc
     3961 gaacttcttc ttgcagccgg gagcgcagaa gtagtaggtc tttcctttgt actggctcgt
     4021 gaattttgcc gtcttttcgt cgactgtcat cttgcatatg gggtcaattg ccatgctttc
     4081 acaccttaag ggagtggtat cgcttcgcag gcggtatata ccttttgagg gagagcgaga
     4141 gggtgacaac ggtcactgac gagagcgcca ttgcaagggc cgcaagctcg gggcggagcg
     4201 tatagccggt gaacgggtag aagatccctg ccgcgagggg aatgagggcc gcgttgtatg
     4261 caaatgccca gaatatgttc tgtcgtatcc gcgacatgac cttcctcgag agctggatgg
     4321 ctgccgcggc atcgagaagg tcgtccctga caagcacgat gtccccgctc tcaatcgcaa
     4381 cgtcggtccc gctcccgata gcgatgccga catcggcccg tgcaagggcg ggggcatcgt
     4441 tgatcccgtc acccacgaac gccacgatat tgcctgcctc ctggagcttt tcaacctcct
     4501 ttgccttgtc tccggggaga acttccgcaa atacctcttc aatcccaagc ctttttccga
     4561 tggctgttgc ggtgagcctg ttgtcaccgg tgatcatcgc caccttcatc cccatcccct
     4621 tcaggacttc cagtgccgac ggcgtggtct ccttgacagt atcggcaacg cccaaaaccc
     4681 ctgcgaggtt gcctccgacg gcgatcagga tgacggtctt cccctcattc tgcagaccgc
     4741 ggaccaccga ttcgcgatcc tgtgcgagca cgaccccctt ctcttccagg aatgcccggt
     4801 tcccgaccag cacctcctct ccgagaaccc gggcagacac ccccttgcca ccgtgggtat
     4861 cgaatccttc tgcctccccg atcggggcac cttccaaaag cgtcctctgt accaccgcgt
     4921 ccgccagggg gtgctgggag ttcttctcga cggctgccgc gattccaaga agtgtctctt
     4981 ggtcgatccc tgaagggaat atgtcggtca ccgcgggctt tcccctggtg agggttccgg
     5041 tcttgtcaaa gacgaccgtg ttcacctggt ccgcagtctc aagcgcctcc ccgttccgga
     5101 caagcacccc aagttcggca cctctcccga ccccgacggt caccgcagtc ggtgtcgcaa
     5161 ggccgagcgc acacggacag gcgaccacca ggacggagac gagagccgtc agggcgaaga
     5221 ggagtgtcga gccgatcacg agataccata cgaggaacgc ggcgaaggcg atcgcgagca
     5281 ctgtggggat gaaatagctg accgccacgt ctgcaatgcg ctggacaggt ggcctcgacg
     5341 cctgcgcgtc ctcgacgaga cggatgatct gggcaagcac cgtgtccttt cccaccttct
     5401 cggccctgaa tgtgaagata ctgttcgtgt tcagggttcc cgcaacaacc tgcgcacctg
     5461 ctgccttgag gaccgggatc ggttcgccgg tgatcatcga ttcgtccacg taactctcgc
     5521 cttctgtcac cgtgccgtca accgggacct tccccccggg ctttacccgg atcctgtccc
     5581 ccacctgtac atcctcaata ggcacctcct tctccccctc gccggtgacc aggagagcag
     5641 tcttcgggcg gagtcccatg agtgcccgga tggccgcaga agtcctcccc tttgccctcg
     5701 cctcgagata tctgccaagg atgaggaatg acgccagcat gatggcggtg tcgtagaaca
     5761 tgaactcgtg cgtgagcact atcccaagcg tcccgaggac actggatacg tatgcaaccc
     5821 ctgtccccat ggcgtacatg acatccatgt tcagggtcct gttccttgcc gccatccggg
     5881 ccgcacgaaa gatgggatag gcaacgtaga tgaataccgg cgtggagacg gccagcatca
     5941 ggtaggagag ggcatgcatg gagattggga ggggaatgta catgagggca aagaggggta
     6001 tgctgactgc gaatcccacc atgaacctcc ggaacttgtc atcgagatcc cgccgccttg
     6061 cctcctcctc ggcttcgacc gtcaactggt cggccagccc gaggtactgg taccccgcat
     6121 cttcgattgc cgccctcatg tcctcgagcg ttgtaagaga ggagttatag gtcacctggg
     6181 ccttctcggt ggcgaggttg acctgcgccc tgacgacgcc cgggagagtc gagagggccg
     6241 tctcgatggt ctccac
//
LOCUS       DAOQVJ010000025         6203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_13968_length_6203_cov_4.277651, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000025 DAOQVJ010000000
VERSION     DAOQVJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13968_length_6203_cov_4.277651"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..3262
                     /locus_tag="PKL26_01020"
     CDS             44..3262
                     /locus_tag="PKL26_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82304.1"
                     /translation="MARVHTCTRGKIPFFLFAEGCGIPWAHYFIILAILFLICPVSPA
                     DLSSPERKEVLYLNSYHRGQEWSDPITDAIEDAFLNSSAPINLHVEYMDSKRFTDDEY
                     VRTLFSLYAHKYRNTRFDALIVSDDNAFKFIMDHRDSLFSGTPVIFCGVNYYSPDMIG
                     ERDGITGVVETFDVKGTLDAALSLLPGTKEVFVINDASLTGAANKKVISDIAPLYAGR
                     LNFTYLEEYTMAELQEKVSSLPDGSVILLMTFNQDRAGDDFSYLETLEYLYPHSRVPI
                     FAIWELYIGHGIVGGMLTSGYEQGRAAASLALEVMNGTDPSEIPVVVGPENHYVFDYR
                     EMQRFGLDVSSLPPGSRILNEPSALVPIPEYLVWAAMISGTCMVLLIVVLLHSNARLR
                     RIRNALDSSERRYRAVVEDQTELIFRTAPDGRIVFANDAFCQFFQRESEEVLAPARNP
                     PIPAGNMDALTKHFQSLSLDNPSGILVDMVILPSGSPRWIRWSNRAIYDEQGVLKEYQ
                     SVGRDFTDLKNAEKELLDYQKTLESEVAARTCELASAYSGLKNEIADRIKVEELLAAE
                     KELLSVTFNAITDGVISTDLQGCVRFMNRAAQEMTGWAVEVAEGQTLDRVFPVVSGGR
                     DSDPDLIAEMHGQCGGLEIQRDLTLLPRNRGELPVSVTVTPIRDRVSRIMGVVIVFRD
                     TTMTQKYEQEQLRSQKLEAIGNLAGGIAHDFNNIVTGILGNISLAKLETPGDTPLYTN
                     LSKAEEAIYRARSLTQRLLTFSRGGAPVKRVVAIGPLVRETTEIMLTGSRSKPTFSIP
                     PDAWSVEADPDQIAQVIQNLVLNADQAMPEGGIIGVTVSNTTLGEGSTLPLPPGHYVA
                     ISVTDQGQGVPEDIQSQIFDPYFSTKAEGTGFGLSAALSIVKKHGGIIDLHSRHGEGA
                     CFTVYLPAATSVKPAESSQEKILPSGSGRILILDDEAGIREILHTILTRQGYQVDEVA
                     EGREAIARFRDCMEGGTPYDLVIIDLTIPGGMGGLGAFRQMRDLEPGVIGIVSSGYST
                     DPVMAEYRSYGFSGVIQKPYRLEEIVNLVARLIKERREKEER"
     gene            complement(3441..4637)
                     /locus_tag="PKL26_01025"
     CDS             complement(3441..4637)
                     /locus_tag="PKL26_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS82305.1"
                     /translation="MTHKVFAKAPTAMKLILLLGVVSLLGDIIYEGGRSVTGPYLLFL
                     GASAFTVAWVSGLGEFIGYAIRLVSGYFADRTREYWTFSLAGYLMIGAIPLLVYARSW
                     EAAVVLLLIERLGKGIRSPAKDTILSHAAHQVGRGWGFGIHEAMDQIGAVAGPLVFAA
                     SLLLLGGYGEGFALLTIPFVLLIAALVLAWRRYPDPASMEESALSFGYTPKDPRVLLP
                     YGAFTVLTMAGFLVFPLMAYHFSAAAVVPDPQIPIFFAVAMATDALAALLMGRLYDRW
                     GLPVIIAIPIANIPIAPLAFSSGYFGALAAAVLWGVSMGGQETVLRAALADFTSIGKR
                     GFAYGIFNTLYGASWFAGSLVFGLLYYVGAGAICAFSIFMQVAAMGAFFLLWRCAGRS
                     SRGYSP"
     gene            4866..5900
                     /locus_tag="PKL26_01030"
     CDS             4866..5900
                     /locus_tag="PKL26_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNS82306.1"
                     /translation="MIGFAWLAILLAFLFTFTNGFQDASSVAATFISSRSATPRQGII
                     FVAAMDFMGALFGGSAVAFTISGLLALEADTHLVPVVLAGLLAALVWNLGTWWTGLPS
                     SSTHGLIGGLVGAGIAAGGLGGVHWGFLEFLAPPHEMTGLTKVVVFLVVSVLLGFMAG
                     YLMRKATRVLLRNAKRMVIRDIVRLNWMAAAVMAFSNGANDSQKQLGIISLVLLSAGL
                     SPSLDIPMWARLGCAALLGAGTLSGGWRIMTTLGRKIFRIEPIHSFDSQVSSGSVLVA
                     STLAGAPVSSSHIISTSVIGVGAAENIRKVQWSVGREIVLVMLVTIPATMVLAFGISL
                     VLFPLTGSIS"
     gene            5897..>6203
                     /locus_tag="PKL26_01035"
     CDS             5897..>6203
                     /locus_tag="PKL26_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNS82307.1"
                     /translation="MTKEKSGRNKGKKGVFEFIFPIEHDFEAMLVEQAEKTLECVYSL
                     VQWLKKTPTDDPQQLMQLEMQLDAMRYDMEDKLMASFSTPFDRQDIYSLSRQMDYIVN
                     "
BASE COUNT         1454 a         1771 c         1699 g         1279 t
ORIGIN      
        1 ctgcaaccat ggatgagtgc aacccgggga agagacagtg ttgatggcca gagtacacac
       61 atgcacaagg ggaaagatcc cgtttttttt atttgcggaa ggatgcggca ttccatgggc
      121 ccattatttc attattctcg ccatcctctt tctcatctgc cctgtctcac ctgctgacct
      181 gtcttcaccc gagaggaagg aggtactcta cctcaactcc taccacaggg gacaggagtg
      241 gtcagatccc atcaccgacg caattgaaga tgctttcctg aactcgtctg ctccgattaa
      301 cctccacgtg gaatacatgg attccaagcg ctttaccgat gacgaatacg tccggaccct
      361 cttttccctc tatgcccaca agtaccggaa tacccgcttc gatgccctca tcgtctcgga
      421 tgacaatgcg ttcaaattca taatggatca ccgggactcc cttttttcgg gcaccccggt
      481 catcttctgc ggagtgaact actattcccc cgacatgatt ggggagagag acggcattac
      541 gggagtcgtc gagacctttg atgtaaaggg cacccttgac gctgccctct cacttctgcc
      601 tggtacaaaa gaggtcttcg tcatcaacga tgcgtccctc accggcgctg cgaacaaaaa
      661 ggtgatcagc gatatcgccc cgttgtatgc agggaggctg aacttcacct acctggagga
      721 atacaccatg gcggagctcc aggaaaaggt ctcctcactt cccgatggga gtgtcatcct
      781 cctgatgacc ttcaaccagg accgggcagg agacgacttt tcataccttg agaccctgga
      841 atacctgtat ccccactccc gagtccctat atttgccatc tgggaacttt atatcggcca
      901 cggtatcgtg ggaggcatgc tcaccagcgg gtatgagcag ggtcgtgctg ccgcttccct
      961 ggctcttgag gtgatgaatg ggaccgatcc ttccgagatc cctgtcgtgg tcgggcctga
     1021 gaaccactat gtcttcgatt accgggagat gcagcggttc ggcctcgacg tttcatccct
     1081 tcccccggga tcgcggattc tcaatgaacc gtcagcgctg gtccccatcc ccgaatacct
     1141 ggtctgggct gcgatgatca gcgggacatg catggtcctc ctcatcgtag tccttttgca
     1201 ttcgaatgca cggttgcgcc gcatacgcaa tgccctcgat tcgagtgagc gccgctaccg
     1261 cgcggtggtg gaagaccaga cggaactgat attcaggact gcccctgatg gaagaattgt
     1321 ctttgcgaac gatgcgttct gccagttctt ccagagagag agtgaggagg tcctcgcccc
     1381 cgccagaaac ccccccatac cggcagggaa tatggatgct cttacgaaac atttccagtc
     1441 cctgtccctc gacaaccctt ccggcatcct cgtggacatg gtaatactgc ccagtggaag
     1501 ccccagatgg atacgctggt ccaacagggc catatacgat gaacagggag tgttgaagga
     1561 gtaccagtcc gtaggcagag actttaccga tctcaagaac gccgagaaag aactcctcga
     1621 ttatcagaag accctggaat ccgaggttgc agccaggacc tgcgagctcg cgagtgccta
     1681 tagcggtctg aaaaacgaga ttgcggaccg aatcaaggtt gaggaactcc tcgctgccga
     1741 gaaagagctc ctctctgtca cgttcaacgc catcaccgac ggtgtgatca gcaccgacct
     1801 ccagggctgc gtcaggttca tgaaccgggc cgcacaggag atgactggat gggccgttga
     1861 ggtggccgaa ggacaaaccc tcgacagagt gttccctgtc gtgagcggcg ggcgggattc
     1921 cgatcccgat ctcattgctg agatgcacgg gcagtgcgga ggcctggaga tacagagaga
     1981 cctcacactg cttccccgta acagagggga actgcccgta tctgtgacgg tgacccccat
     2041 ccgcgaccgg gtttcccgga tcatgggagt ggtgatcgta ttccgggaca ccacaatgac
     2101 gcaaaaatac gagcaggaac agctcaggag ccagaaattg gaagccatcg gcaaccttgc
     2161 cggaggaatc gcccatgatt tcaataatat cgttaccggt atcctcggca acatctcgct
     2221 cgcgaaattg gaaacgccag gggatacacc cctctatacc aacctgtcga aggcggaaga
     2281 agcaatatac agggcgaggt cactgaccca gagactgctc acgttctcac gtgggggtgc
     2341 gccggtcaag agggtggtgg cgatcggtcc cctggtgagg gagacaacgg agatcatgct
     2401 cacaggttcc cgttcaaaac ccactttctc catccctccc gatgcgtgga gcgttgaggc
     2461 agatcctgac cagatcgccc aggtaatcca gaacctcgtc ctcaatgcag accaggcaat
     2521 gccagaagga ggaattatcg gcgtcacggt ctccaatacg acgcttggtg aggggagcac
     2581 acttccactc cctccaggcc actacgtcgc catttctgtc accgaccagg gacagggagt
     2641 acccgaagat atccagtcgc agatctttga tccctacttc tctacgaagg ctgaagggac
     2701 aggttttggt ctttccgcag ccctttcaat cgtgaaaaag catggcggga tcatcgatct
     2761 ccacagcagg catggagagg gtgcatgctt cactgtctac ctcccggctg ccaccagtgt
     2821 gaagccggct gaatcttcgc aggaaaaaat tctcccgagc ggaagcggtc ggatcctcat
     2881 cctggacgat gaggcaggaa ttcgggagat cctccacacc atcctcaccc ggcagggata
     2941 tcaggtggat gaagttgcag aaggaaggga ggccatcgcc aggttcaggg actgcatgga
     3001 gggtgggacg ccttacgacc tggtcataat agatctcacc attcccggtg gcatgggggg
     3061 cctgggagca ttccgccaga tgagagatct tgaaccgggc gtgataggga tagtctcaag
     3121 tggctactcc acggaccccg tcatggcaga ataccgatca tacgggtttt ccggggtgat
     3181 ccagaaaccc taccgcctgg aggagatcgt aaacctggtg gcaagactga taaaagaacg
     3241 gcgggaaaag gaagagagat aaggcgaaaa gcgaactccc tctgcattat ttccgcactg
     3301 gaccagaaaa ggcatcccgg ggtaggtgtc atcaggaaca ggagaataat cttcgcacca
     3361 gcgcatgcaa ggggatcttt aagaataacg ttccccgcct ggctggacca tgaatcccct
     3421 gaataaaacc ctcctttgcg tcatggagaa tacccacgac tacttcttcc tgcgcaccgc
     3481 cagagcagga agaacgcacc catcgccgcg acctgcatga atatggaaaa cgcacatatc
     3541 gctcccgcac caacatagta gagcaggccg aagacgagac tcccggcaaa ccacgaggcc
     3601 ccgtaaaggg tgttgaagat cccgtacgca aaaccccgct ttccgattga ggtgaaatcg
     3661 gcaagcgccg cccgcaggac cgtctcctgg ccccccatcg agacacccca caggactgcg
     3721 gcggcaaggg cgccaaagta gcccgaagag aaggcaagtg gtgcgatagg gatgtttgcg
     3781 attgggatgg cgattatcac cgggagtccc caacggtcat agagccttcc catcaagagc
     3841 gcagcgagcg catcagtagc catcgcaacg gcaaaaaaga tcgggatctg tgggtcaggg
     3901 acaacggctg cggcactgaa gtgatatgcc atcagcggga agaccaggaa ccctgccatc
     3961 gtgagcaccg tgaacgcacc ataggggagg aggacccggg gatcctttgg ggtgtatcca
     4021 aaagagaggg cgctttcctc catcgaggcc gggtcgggat aacgcctcca ggcaagcacc
     4081 agtgctgcaa tgaggagaac aaaaggaatg gtaaggagtg caaatccctc gccatatcca
     4141 cccaacagca aaagggaggc tgcgaagacg agaggtccgg cgaccgcccc gatctggtcc
     4201 attgcctcgt ggatgccaaa accccaccct ctccctacct ggtgagctgc gtgcgagagg
     4261 attgtgtctt ttgcaggcga acgtataccc ttccccaggc gttcgatcag gagcagcacc
     4321 actgcagcct cccatgaacg ggcatagacc agcagcggaa tggccccgat cataaggtat
     4381 ccagcaagag agaatgtcca gtattctcgc gtgcggtccg cgaaataccc tgatacaaga
     4441 cgtatggcgt atccaatgaa ctctccgagg ccagataccc aagccacggt gaaagctgaa
     4501 gccccaagaa acaggagata cgggccggtt acactccggc ccccctcgta aatgatatct
     4561 ccaaggaggc tcactacgcc gaggaggagg atcaacttca tcgccgttgg cgccttcgcg
     4621 aataccttgt gggtcatgat gtgagatcgc tctcatttcc tactttttgt gaagggagtc
     4681 attattgctt gcctctgaat ccccctgaag agaagacccg gcctttgatt caggaaccca
     4741 cgtgccttgc ggggacatct tcttaacatg cccgggtcgt cttacctgtc ataattacct
     4801 cttgtccgga aggcaagcct caaataaatg agagccagaa cctgattgca gtgggaacac
     4861 tatcgatgat tggtttcgcc tggctcgcca tactcctggc ttttctgttt actttcacga
     4921 atggtttcca ggatgccagc agtgtcgccg ccacgttcat ctcttcccgt tcggccacac
     4981 cccgccaggg aatcatcttt gttgccgcaa tggacttcat gggagccctc ttcgggggga
     5041 gcgcggtggc attcaccata tccggcctcc ttgcccttga ggcagacacg cacctggtac
     5101 cggtggtact tgcgggactg ctggctgccc tcgtctggaa cctcgggacc tggtggacgg
     5161 gactcccctc atcatccacc cacgggctca tcggcgggct ggtgggtgcg gggattgctg
     5221 caggcggcct tggcggtgtg cactggggat tcctggagtt cctggcacct ccacacgaga
     5281 tgaccggcct aaccaaggtc gttgtgttcc tggtagtgtc cgtattactc ggattcatgg
     5341 caggatacct catgcggaag gcaaccaggg tcctgctgag aaatgcaaag cgtatggtaa
     5401 tccgggatat cgtcaggctc aactggatgg ccgcggcagt aatggcgttc tccaacgggg
     5461 caaacgactc ccagaaacaa ctcgggatca tttcactggt actcctctcc gcaggtctct
     5521 ccccttccct cgatatccct atgtgggccc gtttgggatg tgcagccctc cttggcgcag
     5581 ggacactcag cggaggctgg cggatcatga ccacgctcgg gaggaagata ttccgcattg
     5641 aacccatcca ttcattcgat tctcaggtat catcgggctc tgtgctggtt gcatccaccc
     5701 tcgcaggggc tcctgtctcc tcaagccaca tcatctcaac atcggtgatc ggggttgggg
     5761 ctgcagagaa tatccgaaag gtgcaatggt ctgtcggaag agagatagtg ctggtgatgc
     5821 tggtgaccat ccctgccacc atggtcctgg cattcgggat ctctctcgtc ctcttccctc
     5881 tcaccgggag tatatcatga ccaaagagaa aagtggtaga aacaaaggga aaaaaggggt
     5941 tttcgaattt atcttcccca tagagcatga cttcgaggca atgctcgttg aacaggcaga
     6001 aaagactctc gaatgcgtat actcacttgt ccagtggctg aaaaagacac cgacagatga
     6061 cccgcaacaa ctcatgcagc tggagatgca gctggatgcc atgcgctacg atatggagga
     6121 caaactcatg gcatcattct ccactccgtt cgaccggcag gacatctaca gcctctcccg
     6181 ccagatggac tatatcgtga act
//
LOCUS       DAOQVJ010000026         6013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_14547_length_6013_cov_4.416247, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000026 DAOQVJ010000000
VERSION     DAOQVJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14547_length_6013_cov_4.416247"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..741
                     /locus_tag="PKL26_01040"
     CDS             34..741
                     /locus_tag="PKL26_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82308.1"
                     /translation="MYTYKTGISVIDEVKGGIFPGSNILVLAPAMSSGEKLGYSIMRP
                     NPGEYALVLSTVMRSVDLLDFFSSEKFNRDSIGVIDAVTKLSTPDISDTAQIKYVSSP
                     SDLTGIGIKFSSIVESIFNGEFPPSDQALFPPPIRFYIDSLTTLLMYRKLEVLYQFLH
                     VMTSKLKKMEAIGIFIMNNESFDERTLSLMKQLMNVVIEVKSEQQGDFLRIRGVPGVS
                     HDWMQFRIHAGTLELVT"
     gene            738..1901
                     /locus_tag="PKL26_01045"
     CDS             738..1901
                     /locus_tag="PKL26_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNS82309.1"
                     /translation="MIRTGITGIDEMLGSGIPKGTRVVFSLEPGVEGRMFMFRTVATV
                     LQERKNVLIVAPHCTGRVLISECSELLNIDISGLEGEVHILDSTVRDTINSREKKTNS
                     RKIAWKKLIRETLDRHSIDVLFIYFDLLYEDLGLEKALDLMPSAGQQDRITTVVEHLN
                     LEGDSLIARFASERIFDLIISVHSAFTLVPFFNFFTLEYVSWSRMPRRSIPYTIGEGS
                     IRLYIPKIIITGPPTSGKSTFVAHASESGISVDRGDLDGYRTTVAMDLGWLHFRGFDI
                     TIFGTPGQPRFDPIIPQIVKNAMGIILLLDATRPDGLLRARELLMLAHAEKLPLVIAV
                     NKSDLPHHLDEKTIRESLNIRDDVPVFFISSLKQSDVHYVVESMVDRITRHPY"
     gene            2034..2783
                     /gene="dph5"
                     /locus_tag="PKL26_01050"
     CDS             2034..2783
                     /gene="dph5"
                     /locus_tag="PKL26_01050"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HNS82310.1"
                     /translation="MLTFIGLGLHDHKDVSMRGLECIRGADHVFLEGYTSRLSGTSVS
                     EMQAYFRRPIRVLGREDVEQHPEIVLDPAATGNVAFLTGGDPMVSTTHADLRIRAALR
                     GIRTVLIHGASISSAVCGLTGLQNYRFGKSCSLPFPMKNWSPATPIEVIGKNLSQGLH
                     TLVYLDIQEERCMTIPEAIVLLEGLAEKAKVSIPLYVGVARAGSGAPVVFAGTAEKVT
                     QADFGPPLHILVVPGPLHVVEEEYLTMFGGL"
     gene            2780..3352
                     /locus_tag="PKL26_01055"
     CDS             2780..3352
                     /locus_tag="PKL26_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HNS82311.1"
                     /translation="MTGRAWRDELSSRLGHLRFYVPPGTILFSVAIDLEGMACSYLRD
                     GDHFESEGDPVNAIASWSYALGWLDAGQSLGLIMAPCHDPRWIYQDIRIASTSGPKLI
                     EKVHRYHALLSTALGAVCPAAEPDTAMSRAADRFTLVAWVNREYGRSFLSSMRTPNAL
                     GAFSYGHAWLDAGVRAGLFRIERSREIFTI"
     gene            3454..4473
                     /locus_tag="PKL26_01060"
     CDS             3454..4473
                     /locus_tag="PKL26_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HNS82312.1"
                     /translation="MKKNEKRWLWVSLGFSLLVLVVILFFTVDANTFALLQKGDPLFF
                     ILALLIHILAMCVWALRIKWMSWGLGYHVPLLHCINLVFANLLVAAITPSQAGGEPVR
                     VHELYKAGVKVGDATAIVITERILDGFVLGLGGAFFMLVLGSIWQQIGSVYSYFLYAS
                     WIMMTAIALLFLYSVKNPLVLKRFLLRATRYVGKIRKKSRMEKYEQMIDREVDNFHNT
                     LSMFISKGKTGLFLGLLCSACFWFLEAVIVSVILIGLGQPPILVESLIIQLIIAIIMM
                     IPLTPGGSGIAEILFTSMYSLFVPTSLIGILVVLWRSILYYSNILLGLLGSLVIVRRE
                     AAEGT"
     gene            4551..4970
                     /locus_tag="PKL26_01065"
     CDS             4551..4970
                     /locus_tag="PKL26_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNS82313.1"
                     /translation="MATGPLGASPAVVLKLGDDETCLPIYIGLWEAISIRNALKQEVP
                     PRPLTHDLFVEFMKSYDIMLKELVIDSLEDGVYYARLIFERDRHEMSMDCRPSDGIAL
                     SLRCGAEILVEPGVFSGSSVCFSDISGLEDLDTYLTG"
     gene            complement(4971..5264)
                     /gene="hisE"
                     /locus_tag="PKL26_01070"
     CDS             complement(4971..5264)
                     /gene="hisE"
                     /locus_tag="PKL26_01070"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNS82314.1"
                     /translation="MTGNDILGELWEVIGNRVQNPREGSYINSLLRDPKGIDRVLEKV
                     GEEATEYILAVKNGRQREIVMEAADLQFHLLVSLKAAGIEFSSVLDELESRRR"
     gene            complement(5251..5703)
                     /locus_tag="PKL26_01075"
     CDS             complement(5251..5703)
                     /locus_tag="PKL26_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HNS82315.1"
                     /translation="MERTIGFKERRYIEELRILTRATALDCIIDERFERVVYVIRQGD
                     MGLAIGKKGENIRRLQNVLGKKVEMVEYSEDIKQFISNMFKPAEVTGIEESEKEGTVD
                     IVVRQKGDLGVAIGKGGATIEKARLLLRRYQGIEIGDVRQEEKPVDRQ"
BASE COUNT         1300 a         1713 c         1588 g         1412 t
ORIGIN      
        1 ttataaacat gctcataaaa tgatgatcga gagatgtaca cctataaaac tgggatcagc
       61 gttattgacg aagtaaaagg gggaattttc cccggcagca atattctggt cctggccccg
      121 gcgatgagca gcggagaaaa acttgggtac tcaatcatgc ggccaaaccc cggggagtat
      181 gcacttgttc tctccacagt gatgcgttct gtagacctgc tcgacttctt ctcttccgaa
      241 aagttcaacc gggattccat cggggtgatc gatgcggtga caaaactctc gacccccgat
      301 atcagcgaca cggcgcagat aaaatacgtg tccagcccaa gcgacctcac gggtatcggc
      361 atcaagttct catccatcgt tgaaagcatc ttcaacggag agtttcctcc ctctgaccag
      421 gccctctttc cacccccgat caggttctac atcgactcgc tcaccaccct cctcatgtac
      481 cggaaactgg aggtgctgta ccagttcctc catgtgatga cctccaagct gaagaaaatg
      541 gaggcaattg ggatttttat aatgaacaac gagtccttcg acgagaggac actttcatta
      601 atgaaacagc tgatgaatgt agtgatcgag gtgaagagcg aacagcaggg tgacttcctt
      661 cgtatccgcg gagtgcccgg agtatcccat gactggatgc agttcagaat ccacgcgggg
      721 accctggagc tggtgacatg atccggacag gaataaccgg aatagacgag atgctcgggt
      781 caggcatacc gaagggcacg agggtcgtct tctcccttga accgggggtc gagggccgga
      841 tgttcatgtt caggactgtc gccactgtcc tgcaggagag aaagaacgtc ctgatcgtcg
      901 caccccactg cactggacgc gtgctcatct cggagtgttc ggagcttctc aatatcgata
      961 tatccgggct tgaaggagag gtacatatcc tggacagcac ggtgcgcgat actatcaatt
     1021 cccgtgagaa gaagacaaat tctcggaaaa ttgcctggaa gaagctcatc cgggagacgc
     1081 tggacaggca ctccattgac gttttgttca tctattttga tctcctctat gaagaccttg
     1141 gtctcgagaa agcactcgac ctcatgccat cagccggaca acaggaccgg atcaccactg
     1201 tcgttgagca tctgaacctg gagggcgaca gcctcatcgc aaggtttgcg tcagaaagga
     1261 tattcgacct gataatttct gtccattccg ccttcaccct ggtgccgttc ttcaacttct
     1321 tcaccctgga atatgtctcg tggtcacgga tgccccgcag gtcaataccg tacacgattg
     1381 gagaggggag catacgtctc tatatcccca agatcatcat cactggtcct cccacatccg
     1441 gaaaatcaac ctttgtagcg catgcatccg aaagcggtat ctccgttgac aggggcgacc
     1501 ttgacgggta ccgcactacc gttgcgatgg acctgggctg gctccatttc agggggtttg
     1561 atatcaccat cttcggcacc cccggccagc cgaggttcga ccccattatc ccgcagatcg
     1621 tgaagaatgc gatgggcatc atcctcctgc tcgacgcaac gagacctgac ggactcctcc
     1681 gggcaagaga actcctgatg cttgcacacg cggagaagct ccccttggtt atcgcggtga
     1741 acaagtcgga ccttccccac catcttgatg agaagacgat cagggagagc ctgaacatcc
     1801 gggacgacgt ccctgtattc ttcatcagct cgctcaagca atccgatgtg cattacgtcg
     1861 tggagtcgat ggtggaccgg atcacccgcc acccttactg aaatggagga tagccgccac
     1921 gtgatgcatc tcccacgaaa ggggtattct caatacatgg aaacgacagg gttaacctca
     1981 cagcaaaaag gatatggcgc atttccgcaa ggaatttaaa aattacagga accatgctca
     2041 cattcatcgg ccttggtctc cacgaccata aggatgtctc catgcgggga ctcgaatgta
     2101 tccgcggggc agaccatgta ttccttgaag ggtacacctc caggctttca ggaacctcag
     2161 tttcagagat gcaggcctac ttcaggcggc caatccgggt tcttggccgc gaggatgtgg
     2221 agcagcatcc cgagatcgtc cttgaccctg ccgcgaccgg caacgtggcc tttctcaccg
     2281 ggggagaccc catggtgtcg accacccatg cagaccttcg gatacgggca gcactccgtg
     2341 gtatcaggac tgtgcttatc catggagcat cgatatcaag cgcggtatgc ggactgacgg
     2401 ggctccagaa ctaccgtttc ggcaagtcct gttctctccc ctttcccatg aaaaactggt
     2461 ctccggcgac ccccatcgag gttatcggga agaacctctc gcagggcctg catacccttg
     2521 tctacctcga tatccaggaa gagcgctgca tgaccatccc tgaagcgatc gtccttctgg
     2581 aggggctggc agagaaggcg aaggtctcca tacccctcta cgtgggagtt gcaagggcag
     2641 gatctggtgc tcctgtcgtc ttcgccggaa cggctgagaa ggtcacgcag gctgatttcg
     2701 gaccgcccct gcatatcctg gtggtgccgg gcccgctcca cgtggtggag gaggagtact
     2761 tgacgatgtt cggggggtta tgaccgggcg ggcgtggagg gacgagctct cctcgcggct
     2821 cggccacctg aggttttacg tccctcccgg gaccatcctc ttctccgtcg cgattgactt
     2881 agaggggatg gcatgttcat acctccgcga cggcgatcac ttcgagtcgg agggagatcc
     2941 cgtaaacgcg attgcatcct ggtcctacgc cctgggatgg cttgacgcag gacaaagcct
     3001 cggcctgata atggccccat gccatgaccc caggtggatt taccaggaca tacgcattgc
     3061 atccacgtca ggcccaaaac tcatcgagaa ggtgcaccgt taccacgcac ttctttcaac
     3121 cgcactcggg gcagtgtgcc cggctgctga acctgatacc gcaatgtcac gcgctgcgga
     3181 ccggttcact ctcgtggcct gggtcaaccg ggagtacggc aggtcatttc tctcttccat
     3241 gagaaccccc aatgcactcg gggccttcag ttacggacat gcatggcttg acgcgggggt
     3301 tcgggccggc ctgttcagga tagagaggtc cagggagatt tttaccatct gaaggcaggg
     3361 ggtcaacagg agtgtcctgg aaattcaaaa cctatatcca ccattacaac ggtacctatt
     3421 gcgttcattt ccctttttcc gcggacgttc atcatgaaga agaatgaaaa acgctggctt
     3481 tgggtgtcgc tcgggttctc cctcctggtc cttgtcgtaa tcctcttctt tacggttgat
     3541 gcgaacacat tcgccctttt acaaaaaggg gacccgctct tcttcatact ggcgctgctg
     3601 atccacatcc ttgcgatgtg tgtctgggct cttcggatca aatggatgag ctggggactt
     3661 ggataccacg tgccactcct ccattgcatc aacctggtct ttgccaacct tctggtcgca
     3721 gccatcacac cctcccaggc agggggagag ccggtaaggg tccacgagct gtataaagcc
     3781 ggtgtcaaag tgggggacgc caccgccatc gtgatcaccg agcgcatcct cgacggattt
     3841 gtgctcgggc tgggtggtgc gttctttatg ctggtgctgg gtagcatatg gcagcagatc
     3901 gggtctgtgt acagctattt cctgtatgca tcatggatca tgatgaccgc aatcgcccta
     3961 ttgtttctct attctgtaaa aaatcccctt gtattgaaga gatttctcct gcgggcaaca
     4021 aggtatgtcg ggaagatacg gaaaaagtcg agaatggaga agtacgaaca gatgatcgac
     4081 agggaggtag acaattttca caacacgctc tccatgttca tttcgaaggg caagaccggc
     4141 ctgttccttg ggctcttgtg ctctgcctgc ttctggttcc ttgaagccgt catcgtctca
     4201 gtcatcctca tcggcctcgg ccagccgcct atcctcgtgg aatcccttat catccagctg
     4261 atcatcgcca tcatcatgat gatccccctc accccgggcg gatcgggaat cgcggagatc
     4321 ctcttcacgt cgatgtacag cctctttgtg cctacttccc tcataggaat ccttgtagtt
     4381 ctctggagga gcatcctcta ctattcgaat atccttcttg ggctcctcgg gagcctcgtg
     4441 atcgtgcgaa gggaagctgc cgaagggacg tgaatacact taagtgcacc ccgggtaatc
     4501 cattgtagca ggctatggat cccgtgaggt gttcagtgag aggggtgtat atggccacgg
     4561 ggccactcgg cgcatccccg gcagtcgtcc tgaaactggg agatgatgag acctgccttc
     4621 ccatctacat cgggctatgg gaagccatct cgatccgcaa cgcactcaag caggaggttc
     4681 ccccgaggcc gctgacccac gatctctttg tcgagttcat gaagtcttat gacatcatgc
     4741 tgaaggaact cgtgatcgac tccctcgagg acggcgttta ctatgcccgg ctcatattcg
     4801 agagggaccg ccacgagatg agcatggact gccggcccag tgacgggatc gcgctctccc
     4861 tgcgttgcgg ggcggaaata ctggtagaac cgggagtatt ttcaggttca tcggtctgtt
     4921 tctcggatat ttccgggctg gaagacctgg atacctacct gaccggctga ctatctcctt
     4981 cgtgactcaa gttcatccag tacggaagaa aattctatgc cggcggcttt caggctcaca
     5041 agcaggtgga actgaagatc ggccgcttcc atcacgatct ccctttgccg gccgttcttg
     5101 actgccagta tgtactctgt agcctcttcg cccaccttct cgagcacccg gtcaatcccc
     5161 ttgggatcgc gcaggagcga gttgatatag gagccttccc gcggattttg cacccggttg
     5221 cctatgacct cccacagctc gccgaggata tcattgccgg tcaactggct tctcctcctg
     5281 ccggacatcc cctatctcga taccctggta cctgcggagg aggagcctgg ccttttctat
     5341 ggtggccccc cctttcccta ttgccacgcc gaggtccccc ttctgcctga ccacgatatc
     5401 cacggtccct tccttttctg attcctcgat cccggtcact tcggcaggtt tgaacatgtt
     5461 ggagatgaac tgcttgatat cttccgagta ttccaccatc tccaccttct tcccaaggac
     5521 gttctggagc ctgcggatgt tctccccctt ctttccgatt gcaagcccca tgtccccctg
     5581 cctgatgaca tagacaaccc gctcgaacct ctcgtcgatg atgcagtcaa gcgcggtcgc
     5641 ccttgtcagg atacgtaatt cctcgatata tcgtcgttct ttaaacccga tagtgcgttc
     5701 catttacctg tccttcatgt atatgtggga gggagcaggg attaaaagat tggcaaaggg
     5761 aacgaaacac cctgtcaaat ttcgcgtgag agagaaaaat catggatacc cccgcacagt
     5821 tccttctatt tccatttctg tacaccgcgg attcgcggaa aaaaaatggg ggaggtgatc
     5881 ttcagcctat aagatacgag gctgtgacct gtgcagtgat cttcacctgc ccgggttcga
     5941 tgggggtcgg gattgccgcc tgcgtcttca tgtcggcagc gcctccccgg acattctcgt
     6001 agaggacagg ggg
//
LOCUS       DAOQVJ010000027         6008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_14558_length_6008_cov_4.898875, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000027 DAOQVJ010000000
VERSION     DAOQVJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14558_length_6008_cov_4.898875"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..676)
                     /locus_tag="PKL26_01080"
     CDS             complement(113..676)
                     /locus_tag="PKL26_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS82316.1"
                     /translation="MSNILLSRRRSLIFISAVKRFIQKTAATIVHGIQSRKGYRKLIK
                     KLNPAVVIREKYSESPGTPTPSFVWTFTAEWKSKTIGTLHLVRYPPEYAPYLGFWLMG
                     LQVEWNYRGLGTGEHLCLAAIDRARVEGAEEIHLLVNERNLPAMQLYEKIGFEQISVP
                     AFDEVLFREKKQTGTTRILMRLTFSNQ"
     gene            789..1109
                     /locus_tag="PKL26_01085"
     CDS             789..1109
                     /locus_tag="PKL26_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNS82317.1"
                     /translation="MQELDMDARYIPSGNIVARVIEGELILVPLVSGIGDIDDELFSV
                     NETGKVIWDRLDGKASLAQIVQLLADQYSVSLGEMEQDVRGFVGELLKRKMIVDARAS
                     PASS"
     gene            1081..1605
                     /locus_tag="PKL26_01090"
     CDS             1081..1605
                     /locus_tag="PKL26_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNS82318.1"
                     /translation="MPEPPLLRHKRYDADEIALSSAALLELLMAVHEKGADFRFRATG
                     TSMYPTIRDGDIITVSPLGGIKPGVYEVVAFRHPGNGKLVVHRVLALSPDSFVAGGDN
                     SQSDGTIPCSHLLGVVTKVERNGSPLFWPDKRRYPFLAKVYFQTGYWYITVRKRGYGM
                     VLKLFRKRSGETIP"
     gene            1602..2732
                     /locus_tag="PKL26_01095"
     CDS             1602..2732
                     /locus_tag="PKL26_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS82319.1"
                     /translation="MTAEFTKVTPLPQYHLWDQTRGKRIPYSFDLELTARCNNSCRHC
                     YINLPRNDRAAIKKELTPEEVASIADQAVSLGSLWCLITGGEPLLREDFSDIYTMLKK
                     KGLLVGVFTNACLISDEHIQLFRTYPPRDIEVTVYGATKETYEKVTRRPGSYEAFRRG
                     LDLLLEGGVKVRLKAMALRSNVHELPEISRFCRTYTKDYFRFDPLLHLRYDGNKKRNA
                     EIMVERLSPDEVVAIEQADQERADSLLRGCNELIIPALKNEESDRLFTCGAGRGSFTV
                     GHDGYFRLCSSLWHPGSVYDLRKGSLKDAWEHFVPQVLDLRSSDRDFLEKCRKCPIIN
                     LCLWCPAHASLESGRMDAWCQYFCDVAHARAKALEKRAGLSQ"
     gene            2729..4567
                     /locus_tag="PKL26_01100"
     CDS             2729..4567
                     /locus_tag="PKL26_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS82320.1"
                     /translation="MRSTTLPPLKISTLHQTLLRALRLVWSMAPGWTAANMLLVVIMG
                     VLPIAALYLTKVIVDSVTAGLAGTDPMVLFPQVFFYILVAAAIALSLAIARSLSELTS
                     EAQSMIVTDGVSDILHAQSIAVDLEYYEDTSYYDTLHRAQQEAPYRPTRIVNGLVQIG
                     QNGISLLGVAVLILTFSWVIALLLIAAAIPAALVRYYYARARFRFEQENTESERQAGY
                     YHWVLTDSSHAKEVRLFDLGPLFRDRFRNFRADLRKGRLNLARYRTLSDSASQAIVVI
                     ALFGTFAFIAYSTLQGMITVGDLVMYFLAFQLGIGFIQAILTSLAGLYEDNLFLANFY
                     QFLDLTPVIQAPEIPVRIPEPRDQKIEFKDVRFSYPGGTKDAVSGVNLAMNPGEVIAL
                     VGENGSGKTTLIKLLCRLYDPGSGSITVNGIDLRHFDPVAWRKEISVIFQDYVHYYLS
                     AGENIWVGDVERDQDPATITQAANRSGADTVIQKLPEGYKTNLGHWFSGGQELSEGEW
                     QKVALARAFFRDSNIVVLDEPTSSLDPLAEADLFRNFRDLLEGRSAILISHRFSTVQM
                     ADRIYVLDKGQIIEAGSHEELLKLNGRYAHLFRTQAEHYQVKHSEH"
     gene            4889..5275
                     /locus_tag="PKL26_01105"
     CDS             4889..5275
                     /locus_tag="PKL26_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNS82321.1"
                     /translation="MKIQYDTILSRLQRLEGFSHVRFIILFGSVAEGRELPGSDIDLC
                     ISYKGTVEEASRFRVAALTELSDTPCDIRIFEQMPLLMRLQVFAGEVLYCPGPDLLYS
                     VAYETIRDYEAFRHRLSDYTGEKAIL"
     gene            5275..5574
                     /locus_tag="PKL26_01110"
     CDS             5275..5574
                     /locus_tag="PKL26_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNS82322.1"
                     /translation="MKKKLRRDLIHVKIQEIREGIHLVSLHLPDSYEKFSQLGLVKDG
                     IYKRTEYAIETVLDICAILNSDLKLGIPGEDEDSITHLVQGGILSHDMAEKIRGM"
     gene            complement(5784..>6008)
                     /locus_tag="PKL26_01115"
     CDS             complement(5784..>6008)
                     /locus_tag="PKL26_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNS82323.1"
                     /translation="QDAVIRNLEIIGEAVKHISETVKETSPDIPWKQIAGLRDLLIHH
                     YMGVNLDEVWNIVVNDIPALKIALISLQPK"
BASE COUNT         1390 a         1521 c         1603 g         1494 t
ORIGIN      
        1 atctcggact catcgaaatg ggtgagtctg catgggaagt gacggatggc agagaaaggg
       61 ttgaacggat gacaaaggaa ctcatcaagt agaaaaatat tcagaagcgg gattactggt
      121 tactgaaggt caggcgcatc aggattcgcg ttgttcccgt ctgctttttt tccctgaata
      181 gcacttcatc gaaggccgga acagatatct gttcaaatcc aattttttcg taaagttgca
      241 ttgccggcag gtttctctca ttcaccaaga gatggatctc ctctgctcct tcgactctcg
      301 ctctgtcgat tgctgcaaga cagagatgct ctccggttcc caatccccga tagttccatt
      361 caacctggag gcccatcagc caaaatccta ggtagggcgc atactcaggg ggataccgaa
      421 ccaagtgaag agtgccaatg gtcttggatt tccactcagc ggtgaaagtc catacaaaag
      481 agggggtagg agttcctggg gactcggaat atttctccct gataacaacg gccgggttta
      541 atttcttaat gagttttcta tacccctttc tgctctgaat accatgaacg atggtggctg
      601 ctgttttttg tatgaatcgt tttacggctg atataaaaat taagctcctc ctcctcgaca
      661 ataatatatt actcatttac aatggaaaac atgatactga ttctctccaa tatattccgc
      721 tagaatcaga tattcacacc gcttatcaga cttatctcaa tctcttggtt acataaggag
      781 gaacaaggat gcaggaactt gatatggatg cccgttacat cccgtcgggt aatatcgttg
      841 caagggtaat tgagggagaa ttaattctcg ttccccttgt ctcgggaatt ggagacatcg
      901 atgacgagct tttctccgtg aacgagacgg gaaaagtgat ctgggaccgg ctcgatggaa
      961 aggcaagtct tgcccagatt gtccagctgc ttgcggacca gtactcggta tcattggggg
     1021 agatggagca ggatgttcgc ggatttgtgg gagaacttct caagagaaag atgattgtcg
     1081 atgcccgagc ctcccctgct tcgtcataag aggtatgatg cggacgagat cgcactttcc
     1141 agtgccgccc ttctcgaact cctcatggcg gtccatgaaa aaggtgctga tttccggttc
     1201 agggcaacag ggacgagcat gtatcccacg atcagggatg gtgatatcat tacggtatca
     1261 cctctcggag gaatcaagcc aggtgtgtac gaggtcgttg cattccgcca tccgggcaat
     1321 ggtaagctgg tggtgcatcg tgttcttgcc ctgtcccccg attcgtttgt ggcggggggt
     1381 gataacagtc agtcagatgg aaccataccc tgctctcatc tccttggtgt ggtgacgaag
     1441 gttgaacgga acggatctcc cctcttctgg ccggacaagc gccggtatcc attcctggca
     1501 aaggtgtatt tccagaccgg gtactggtat attacggtga ggaagagagg gtatgggatg
     1561 gtcctgaaac tcttcagaaa gcgatcaggg gagacgatcc cgtgacggca gaattcacca
     1621 aggtgacgcc actccctcag tatcacctct gggaccagac caggggaaag cggatcccct
     1681 actccttcga tcttgaactg accgcccgct gcaataactc ctgccggcat tgttacatta
     1741 accttccccg gaatgacagg gctgcgatca agaaagagct gacccctgaa gaagtagcgt
     1801 ctatcgctga ccaggcggtc tccctcggat cactctggtg cctgattacc ggcggcgaac
     1861 cactgctccg ggaggatttt tctgacatat ataccatgtt gaagaaaaaa gggctccttg
     1921 tcggcgtttt taccaatgca tgcctgatca gcgatgaaca tatccagctt tttagaacat
     1981 atcctccccg ggacatcgag gtcacggtat atggtgccac caaggagacc tatgagaagg
     2041 tgacccgccg gccgggttcg tacgaggcat tccggcgggg tcttgacctt ctcctggaag
     2101 gaggggtgaa ggtcaggctg aaagccatgg cgctccggtc gaacgtgcat gaacttccgg
     2161 aaatttcccg gttttgccga acgtatacca aagattattt ccgcttcgat ccgctgctgc
     2221 atctcaggta tgatgggaac aaaaaacgga atgccgagat catggtggag cggttgtcgc
     2281 cggacgaggt ggtagccatt gaacaggcgg atcaggaacg ggcagattcc ctgctccgtg
     2341 gatgcaatga actgattatc ccagccctga aaaacgagga aagtgatcgt cttttcacgt
     2401 gcggggccgg gagaggaagt tttacggtcg gccatgatgg ttatttccgt ctctgctcct
     2461 cgctctggca cccgggatca gtctatgatc tccggaaggg ttcgctgaag gatgcctggg
     2521 aacattttgt tccccaggta ctggatctcc ggtcatcaga ccgggatttt cttgaaaaat
     2581 gcaggaagtg cccgatcatc aacctctgtc tctggtgtcc tgcccatgcc tctcttgaat
     2641 ccgggcggat ggatgcctgg tgtcagtatt tctgtgatgt tgcccatgcc cgggcgaaag
     2701 cattggagaa gcgggcagga ctgtcgcaat gaggagcacg actctccccc ccctgaagat
     2761 ctccacgctc caccagacgc tgctccgggc attgcggctg gtctggtcca tggctcccgg
     2821 ctggaccgca gcaaacatgc tcctcgtcgt catcatggga gttcttccca tcgcggctct
     2881 gtacctgacc aaggtgatcg tggattccgt cactgctggt cttgctggaa ctgacccaat
     2941 ggtactcttt ccccaggtgt ttttttacat cctggttgct gctgcgatcg ccctgtccct
     3001 tgccattgca cggtcgctct ccgagctgac ctcagaagcg cagtcaatga tcgtcaccga
     3061 tggagtatcg gatatcctcc atgcgcagtc aattgccgtt gacctggaat attacgagga
     3121 taccagttac tatgataccc tgcaccgggc ccagcaggaa gccccgtacc gcccgacccg
     3181 gattgtcaac ggcctggtcc agatagggca gaatggcata tcgctgctcg gggtcgctgt
     3241 gctcatcctc acgttcagct gggtgattgc tctcctcctt attgctgctg ctatcccggc
     3301 cgctcttgtg aggtattatt atgcccgtgc ccggttccgc ttcgaacagg agaataccga
     3361 gagtgaacgc caggcagggt actatcactg ggtactgacc gactcttccc atgccaagga
     3421 ggtccggctc ttcgacctcg gcccgctctt ccgtgatcgg ttccggaatt ttcgtgctga
     3481 tctccgcaaa gggcgattga atctggcccg gtaccggaca ctctccgata gtgcatcgca
     3541 ggcgatcgtg gtcattgctc tgtttggaac gtttgccttc atcgcctact caacgctcca
     3601 gggaatgatc acggttgggg atctcgtgat gtatttcctc gcgttccagc tcggcatcgg
     3661 tttcatccag gcaatcctca cttccctggc aggactctac gaggataatc tcttccttgc
     3721 caacttctac cagttcctgg atctcacgcc ggtgatccag gcccctgaaa ttcccgtcag
     3781 gattcctgaa cctcgcgacc agaagatcga gtttaaggat gttcgcttct cttatcccgg
     3841 ggggacaaag gatgcagtga gcggagtgaa tcttgcgatg aatccaggag aagtcattgc
     3901 cctggtcggt gagaatggtt cagggaagac tacgctcatc aagctcctct gccggttgta
     3961 tgatcctggc tccgggagta tcacggtgaa cgggattgat ctccgtcact tcgacccggt
     4021 tgcctggcga aaggagatca gcgtgatctt ccaggattat gtccattact atctctctgc
     4081 gggtgagaac atctgggtgg gggatgtcga acgggaccag gatcctgcca ctattactca
     4141 agcggccaat cgttcggggg ctgatacggt catccagaag ctccctgagg gatataagac
     4201 caacctgggc cactggttct ccggagggca ggagctgagc gagggcgagt ggcagaaggt
     4261 tgccctcgca cgggcgttct tccgggactc gaacatcgtg gtgctggatg aaccgaccag
     4321 ttccctcgac cccctcgctg aagccgatct cttccggaac ttccgggacc tgctggaagg
     4381 ccggagtgcc atcctgatca gccaccggtt ctccaccgtc cagatggcag accggatcta
     4441 tgtccttgac aagggacaga tcatcgaggc gggaagtcat gaggagcttt tgaagttgaa
     4501 cgggaggtat gctcacctgt tcaggaccca ggctgagcat taccaggtga aacattccga
     4561 acactaagga gcaagctgcg gtggaaccga caattcggga ggatgtcatc tttgcggaaa
     4621 aaacagaaca agcatttctg gaatataaaa aggggagagt aacgacagaa tccgatcatg
     4681 attttattga taccctcgca tcatggtgac ggttgcattc tgatttttta aaatttaaaa
     4741 agcgatcaaa ccatgaatag tgtaccgatc ggaacagaat gatgaggatt cagtttgcag
     4801 caactcctgg aaggcttgta ccgttgcgat gcccggatac ctttaaaggt tttcagcatg
     4861 taattttcag ataaagaggg aatccgacat gaaaattcag tatgacacca ttctctctcg
     4921 gttacaaaga ctggaaggat tctctcacgt ccgttttatc attctcttcg ggtcggttgc
     4981 cgaagggagg gagctaccgg gatcggatat cgacctctgt atctcctata agggtacggt
     5041 ggaagaggca tcacggttcc gggttgctgc cctgaccgag ctctcagata ccccctgtga
     5101 tatccgaatc tttgagcaga tgccactcct catgaggctc caggtctttg ctggcgaggt
     5161 tctctattgc ccgggccctg accttctcta ctcggtagcc tatgaaacca tcagggacta
     5221 tgaggcattc aggcatcggt tatcagatta taccggtgag aaggcgatac tctgatgaag
     5281 aaaaagctcc gtcgtgatct cattcatgta aaaatccagg agatacggga agggatccat
     5341 cttgtatcat tgcaccttcc cgactcttat gaaaagttct cccagcttgg tcttgtgaag
     5401 gatggtatct acaagcggac cgagtatgcg attgagaccg tcctcgacat ctgcgccatc
     5461 ctgaattcag acttgaagct ggggattccc ggtgaagatg aggatagtat tactcacctc
     5521 gttcagggcg ggatcctctc tcacgatatg gcggaaaaga tccggggtat gtaagcattc
     5581 caaaacattg tcgtgcaccg ttatggtacg atcgatgacc aaatagccta caccctgctc
     5641 gttgaacgaa tcggtgattt cgaattattt tgcagggata tcgagcattg tcttgccagg
     5701 gactaaaaaa agagatcctg gtgtaaaaac aagggagcat gcatccgtcc cggacaggag
     5761 agtcccgagc ggagacaaac gggctatttt ggttgcaatg aaataagagc gattttcagc
     5821 gcagggatat cattcaccac gatattccag acttcatcaa gattcacacc catgtagtgg
     5881 tgaattaaaa ggtcacggag ccctgcgatc tgtttccagg gtatatcagg ggacgtctct
     5941 tttacggttt ctgaaatatg ttttaccgct tctccaatga tttcaagatt tcttatcacc
     6001 gcgtcctg
//
LOCUS       DAOQVJ010000028         6000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_14587_length_6000_cov_5.008074, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000028 DAOQVJ010000000
VERSION     DAOQVJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14587_length_6000_cov_5.008074"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..2487
                     /locus_tag="PKL26_01120"
     CDS             337..2487
                     /locus_tag="PKL26_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HNS82324.1"
                     /translation="MGLVPSPIVLKSGTGDLKVPVYSDSKKSATALNETTSLILKSIE
                     QYRICPMNPEFRRFIEEYNSKKTQESDPEDRSLGLIPSPIDIGALTVSDTLGSDQAPV
                     SIVGSTAYGYYPSSITTATPSSWFDLRMTGKLTPVRNQEQCGACWAFSTYGSLESTNL
                     PGIRWDFSENNMKNTHGFDLAPCKGGNYLMSTAYLSRWSGAVSESSDPYVTTESSSPG
                     SVHVVQHVQDVIFLPSRTGPLDNSQIKQLLQQNGAVYSQIRWESGYFNKNLASYYYPG
                     SSSPNHAVVIVGWDDTYSRMRFSSEAPGDGAFIVRNSWGENWGEYGYFYVSYYDTCIG
                     RPSAQFLSEDPSNYDKVYQYDPLGWVTSVGVGGNSAWFANVFTSTQAEELDAVSFYSP
                     VPNTQYQLEVCSDVGSSPRGRSSILAQAGSIPYAGYHTIALSNPVALQKGQKFSVIVN
                     LITPGCSYPIAVEYPYQGYSSKATARAGESWVSSDGTSWTDITSVYPNSNVCLKAFTQ
                     ITTNPTYNPTVQPTGIPTMPSAGTDRRSPTISISSPKFMASYAPGDEVRIAWSAQDNI
                     AVSSVMVEYSPDKGLNWIPILQHAPATGTCPWAVPENLAGSITIRVTASDAAGNKGSS
                     SRSITVKSSAAASPFSWSQQKVSPGLGRNLTPPVALVSVSAGADPMTFSRYDSREQTF
                     ISPVSRSAASGGSLSFVSPREKTTTPTLLEQRAI"
     gene            2503..3495
                     /locus_tag="PKL26_01125"
     CDS             2503..3495
                     /locus_tag="PKL26_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNS82325.1"
                     /translation="MPRDRSLSRGCVLCHQGAKMVLFVTGLCPRSCWYCPLSRERKGK
                     DVIYANEKRIHTPEEAAGVARLMSAFGTGITGGEPLIVLDRVQQFSRHLKTEFGESHH
                     IHLYTGIAPGREQLLDLQGLVDEIRMHPPYETWDRILESGYKEAACTAREMEFSVGIE
                     VPSLPGIGSLEVMLPFLDFLNINELEWGETNAGAMRERRLVPEDGVHNAVSGARGWAK
                     SLISRQKVHWCSSRFKDSVQLRKRLLRVARNTARPFDQITRDGTVLYGTLENGGVVPP
                     LFRQLEPDMYEVREGAIETAWWVITEFSDQLAGEKSIVERYPDRGLVVEVTPVA"
     gene            3492..4307
                     /gene="uppS"
                     /locus_tag="PKL26_01130"
     CDS             3492..4307
                     /gene="uppS"
                     /locus_tag="PKL26_01130"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNS82326.1"
                     /translation="MIRELLSPLYERIVRLQCRHIPHHIAIIQDGNRRYARLQHLPRS
                     MGHRAGAARTEEMLDWSRELGIRHITLYSFSTENFQRDAGEVADLFGLFKEKFQKVTD
                     DPRVHRDGIRVQIVGDRALLPADLREVIEKAEESTRSYTGYHLNIALAYGGRNEIVRA
                     AGRILDGVKAGEYTPDEITPELVETHLHAGKDIPPVDLIIRTGNECRTSNFLPWLAGG
                     HESAVYFCAPFWPLFRKIDLLRAIRVYSQRVEAQRGMHIAAGAYTSLPETADT"
     gene            complement(4281..4925)
                     /locus_tag="PKL26_01135"
     CDS             complement(4281..4925)
                     /locus_tag="PKL26_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HNS82327.1"
                     /translation="MIRWLYERRLLSGLSVLPAQICFMITGDDVEEAPGKLVEVTRWC
                     VEISEAVAHAGRAPGVKGGPGGITGVTFHISTRGRDEGFSSIGRIREVAGFARLTLHH
                     GETKETMGEGMDVHVAIGKSGREEITECIRKMARDGLVNEEVSEHAIEERLTFRYTPD
                     LVIKTGGSHLTDFLIWQSVYSELFFSDVNWALFRKTDFLRALRDYQSRVRRFGK"
     gene            complement(4922..>6000)
                     /locus_tag="PKL26_01140"
     CDS             complement(4922..>6000)
                     /locus_tag="PKL26_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNS82328.1"
                     /translation="LNRRLRSIADEMIDEGERKSSFWCRGSRVDVRYTLSSTYGSMLL
                     YLTGAKAFNIRLREIALGKGWRLNEYGIRDLARGTVHEFSREEDIFAFLGMDIIPPEL
                     REDWGEIDAALSHRLPALIEGSHVRGDLHVHSSWSDGSMDLEALARAGEACGYSYLLC
                     TDHSSSLGVARGLDESALKRQSHEIEMVNRATSCQILHGVEVDILADGTTGLPSRVLK
                     DLDVVVGSVHTAFTQETDLMTRRVLSAIGNEHVDIIGHPTGRLLGQREAYAIDMQRVI
                     ESAAETGTALECNASPYRMDIDDVHIREAVNHGGFISIGTDAHGPGDLAWMNYGVRLC
                     RRGWAGPGNVINTLNMHDLLEWAS"
BASE COUNT         1379 a         1766 c         1626 g         1229 t
ORIGIN      
        1 gaaccatccc catcctatca ttttatcatg atatcaggat acgacatacc cagaggcgac
       61 tggaatgcag aattaagtat ctgaatgata catcatatcc agggaccgcg gaggaattct
      121 gcgccagtca ggatagaaca acgattcaat acgggatgaa tgcaaatggt taaaaaagta
      181 ataatttttc ttgcgatctg ctcagtgctg ctattccagg tctctgcaga agacaggaca
      241 tacctggaaa agccggatgt gagtcccgat ttttttacat atttcaaata caacgcgagc
      301 gttggaggca caacgtttga cagggaaggt catagcatgg gcctcgtccc atcgcccatt
      361 gtcctgaaat cggggacagg agatctgaaa gtccccgtct attcggactc aaagaagtct
      421 gcaacggcct taaacgagac cacctccctg atattgaaat ccattgaaca ataccggata
      481 tgccctatga accccgagtt caggagattc atcgaggaat ataactcgaa gaaaacgcag
      541 gagtcggacc cggaagatcg ctcccttgga ctcatccctt ctcccataga tatcggcgca
      601 ctcactgtat ctgatacctt gggatctgat caagcgcccg tttccatcgt tggatcaacg
      661 gcatacggtt attatccatc atcaatcacc actgctaccc catcaagctg gttcgacctg
      721 cgcatgacag gaaaactcac tcctgtccgg aaccaggaac agtgcggggc ctgctgggca
      781 ttctccacat atggttctct cgaatccacg aacctgcccg ggatacggtg ggacttttca
      841 gagaacaaca tgaaaaacac gcatgggttc gaccttgcac catgcaaggg aggaaattac
      901 ctcatgtcca ccgcgtacct ctcacgctgg tcaggggcgg taagcgagtc gtccgatccc
      961 tacgtcacga cagaaagcag ttcgcccggg agtgtccacg ttgtgcagca tgtccaggac
     1021 gtcatattcc tgccctcaag gacagggcct ctcgataact cccagataaa gcagctgctc
     1081 cagcagaatg gagcagtgta ttcacagata cggtgggaga gcggatactt caacaaaaac
     1141 cttgcatcct attactatcc cggctcatcc tctccaaacc acgcggtagt aattgtcgga
     1201 tgggacgaca cctacagcag gatgaggttc tccagtgaag cacccggcga tggggcattc
     1261 attgtcagga acagctgggg tgaaaactgg ggtgagtatg gatatttcta cgtctcctat
     1321 tatgacacat gcatcgggcg gccttccgcc cagtttcttt ctgaagaccc gtccaattat
     1381 gacaaggtct accagtatga cccgcttgga tgggtgacga gcgtcggggt agggggaaac
     1441 agcgcatggt ttgccaacgt ctttacgtcg acacaggctg aagagttgga tgcggtaagc
     1501 ttctattcac ctgtcccgaa tacccagtac cagctcgagg tatgcagcga cgtgggatcc
     1561 tcaccccggg ggagatcctc aatccttgcc caggcaggga gtatcccata tgcaggctat
     1621 cacacaattg ccctctccaa cccggttgcc cttcagaagg ggcagaaatt ctccgttatc
     1681 gtcaatctta tcacgccggg atgcagttac ccaatcgcgg ttgaataccc ttaccaggga
     1741 tactcatcga aggcgaccgc acgggcaggg gagagctggg tgagcagcga tgggacgagc
     1801 tggacagata tcacgtcagt ctacccgaat tcaaacgtgt gcctgaaggc attcacgcag
     1861 ataacgacaa accccacgta caaccccact gtccagccga ctggaattcc caccatgcct
     1921 tccgcaggga ctgacaggag aagcccgaca atcagcatct cttccccgaa atttatggcc
     1981 agttacgcac cgggagacga agtcaggatt gcatggtcag cgcaggacaa tatcgccgtg
     2041 tccagtgtga tggtagaata ttctcctgac aagggcctga actggattcc cattctccag
     2101 cacgctcctg caaccggcac ctgtccctgg gctgtcccgg agaaccttgc ggggagcatc
     2161 acaatccgcg taaccgcaag cgatgccgca ggaaataagg gttcctcaag caggtccata
     2221 accgtcaaat ccagtgcagc cgcaagtcca ttctcctggt cgcagcagaa ggtgtctccg
     2281 gggttgggtc ggaatctgac accaccggtt gcactcgtga gcgtatctgc cggcgctgac
     2341 cccatgacat tttcccgcta cgacagcagg gaacagacct tcatttcacc ggtctcccga
     2401 tccgctgctt ccggagggag tctttcgttc gtatcaccca gggaaaagac cacaactcca
     2461 accttattag aacagagagc gatataatcg gaaggaagag agatgccaag agacaggtcg
     2521 ctctcccggg gatgcgtgct ctgccaccag ggtgccaaga tggtcctctt tgtcacgggc
     2581 ctctgccccc gctcctgctg gtactgcccc ctctcccggg aacgaaaggg gaaggatgtg
     2641 atctatgcca acgagaagcg catccatact ccggaagaag ccgcaggggt cgcccgcctc
     2701 atgagcgcgt tcggcacagg cattaccgga ggggaacctc tgattgtcct tgacagggtg
     2761 caacagttca gccggcacct gaagacggag tttggggaat cgcaccacat tcacctctat
     2821 acaggaattg caccagggag agagcagctt ttggatctgc agggactggt cgacgagatc
     2881 cggatgcacc ccccatatga gacatgggac aggatccttg agagcgggta taaagaggcg
     2941 gcatgcactg ccagggagat ggagttctca gtggggatcg aggtcccctc gctcccgggc
     3001 atcgggagcc tcgaagtgat gcttccgttc ctggactttc tcaatatcaa cgaactcgaa
     3061 tggggtgaga ccaacgccgg ggccatgcgc gagcgtcgcc tggttcccga ggatggagtg
     3121 cacaacgcgg tcagcggcgc acgaggatgg gcaaagagtc ttatcagccg gcagaaagtg
     3181 cactggtgtt catcgcggtt caaagactct gtccagctca gaaagaggct gctccgggtc
     3241 gcccgcaaca ctgcgaggcc atttgaccag attacacgcg acggtaccgt tctctatggt
     3301 acccttgaaa acgggggggt cgttcctccc ctcttccgtc agcttgaacc tgacatgtat
     3361 gaagtgaggg agggtgccat cgagaccgcc tggtgggtaa tcaccgagtt ttccgaccag
     3421 ctggcagggg agaagagcat cgtggagcga taccccgaca ggggccttgt cgtggaggtg
     3481 acaccggttg catgatccgc gagctgctct ccccactcta tgagcgcatc gtgcgcctcc
     3541 agtgccggca cattccccac cacatcgcca tcatacagga cgggaaccgc aggtatgcca
     3601 ggctccagca cctcccgcgc tcaatggggc atcgggcagg cgccgcacgg actgaggaga
     3661 tgctggactg gtcacgtgaa cttggaatcc ggcatatcac cctttactca ttctcgacag
     3721 aaaacttcca gagagatgca ggggaggtcg cggatctctt tggcctcttc aaggagaagt
     3781 tccagaaggt caccgacgac ccgagggttc accgcgacgg catcagggtg cagatcgtgg
     3841 gtgaccgggc actcctcccg gcagacctgc gcgaggtgat cgagaaggcg gaagaatcca
     3901 cacggtccta cacaggctac catctcaaca tcgcgctggc ctatggtggc cgaaacgaga
     3961 tcgtgcgggc cgccgggcgg atcctggatg gggtaaaggc aggagaatac actcctgacg
     4021 agattacccc cgagctcgtc gagactcatc tccatgcagg gaaggacatc ccccccgtcg
     4081 acctgatcat tcgcacagga aacgagtgca ggacctcgaa tttccttcca tggcttgcag
     4141 gcggccacga gtccgcggtc tatttctgtg ccccattctg gcctttgttc cggaagatcg
     4201 atcttctcag ggcgatacgg gtatactccc agagagttga ggcacagcgg ggcatgcaca
     4261 tcgctgcagg ggcatacaca tcacttcccg aaacggcgga cacgtgactg gtagtcccgg
     4321 agcgcccgta aaaagtctgt cttcctgaaa agcgcccagt tcacgtccga gaagaagagc
     4381 tcggaataca ccgactgcca gatgaggaag tcggtcaggt ggctcccacc tgtctttatc
     4441 acaaggtcag gagtataccg gaaggtgagc cgctcctcga tggcgtgttc cgacacctcc
     4501 tcattcacaa gtccatccct cgccatcttc ctgatgcact cggtgatctc ctcccttccg
     4561 ctcttcccta ttgcgacgtg cacgtccatc ccttccccca tcgtctcttt ggtctccccg
     4621 tggtgcaggg tgagtcgtgc aaagccggca acctccctga ttctgccgat gctggagaat
     4681 ccctcatccc tgccccgggt gcttatatgg aatgtcaccc ccgtgatccc gcctggccca
     4741 cctttcacgc caggtgccct gcctgcgtgg gcaaccgcct ccgaaatctc cacgcaccac
     4801 cgcgtgactt cgaccagctt gcctggcgcc tcttccacgt catctcccgt tatcatgaag
     4861 cagatctggg cagggaggac cgagagccct gaaagaagcc ttcgttcata cagccaccgg
     4921 atcatgatgc ccactccagc agatcatgca tattcagggt attgattaca ttcccgggtc
     4981 ctgcccatcc cctccggcag agccgcaccc cgtaattcat ccaggcaagg tcgccggggc
     5041 catgggcatc ggtgccgatc gagataaaac ccccgtggtt cacggcctcc ctgatgtgaa
     5101 catcatcgat gtccatccgg taaggtgaag cattgcattc gagtgccgta cctgtctctg
     5161 ctgcagattc gattacccgc tgcatatcga tcgcgtatgc ctcccgctgg ccaagcagtc
     5221 tcccggtagg gtggccgatg atatcaacat gttcattccc gatagcggag aggacgcgcc
     5281 gtgtcatcag atcagtctcc tgggtaaatg ccgtgtgcac cgatccgacg accacatcga
     5341 gatctttgag aacccgggag ggaagcccgg ttgtcccgtc tgcaaggatg tccacctcga
     5401 ctccatggag aatctggcag gaggtggcgc ggttcaccat ttcaatctcg tgtgactgcc
     5461 gcttgagcgc ggattcgtcg agtccgcggg caaccccgag ggatgacgag tgatcggtgc
     5521 agaggagata gctataccca cacgcctctc ctgcacgggc gagagcctcg agatccatgc
     5581 tcccgtcact ccaggaactg tgcacatgga ggtcccctct cacatgggac ccctcgatca
     5641 gagcaggaag acggtgcgaa agggcagcat cgatctcgcc ccagtcttcg cggagttccg
     5701 ggggaattat gtccatcccg aggaatgcaa agatatcctc ctcgcgggag aattcatgga
     5761 cagtacctcg ggcaaggtcg cggattccat actcgttcag ccgccatccc ttcccaagcg
     5821 cgatctccct gaggcggatg ttgaaggcct tcgcacccgt gaggtagagg agcatggagc
     5881 cgtacgtgga cgaaagagta tacctgacat ccactctcga accacggcac cagaacgagc
     5941 tcttcctctc gccttcgtca atcatctcgt ctgcaatgct ccgaagtcgc ctgttgagaa
//
LOCUS       DAOQVJ010000029         5936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_14781_length_5936_cov_3.723346, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000029 DAOQVJ010000000
VERSION     DAOQVJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14781_length_5936_cov_3.723346"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..844)
                     /locus_tag="PKL26_01145"
     CDS             complement(56..844)
                     /locus_tag="PKL26_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS82329.1"
                     /translation="MTYDDWLRADRLPHIYCAGCGNGTVINCTLNAVDRMGWKKEETI
                     FVSGIGCSSRAPGYIMTDSLHTTHGRALAFATGIKMANPDLHVVVFTGDGDLAAIGGN
                     HFIHACRRNIDLTVVCMNNQIYGMTGGQGSPTTPRGALSTTTPYGSQEPAFDLCELAS
                     AAGANFVSRWTSYHVKELTRSIHEGLGIPGFSFIETLVQCPTNYGRRNKFRQVMDQVE
                     YFKAHGILVQKAARLREEGKAIPGDAIILGDIVRRDRPAMGVRR"
     gene            complement(841..1941)
                     /locus_tag="PKL26_01150"
     CDS             complement(841..1941)
                     /locus_tag="PKL26_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNS82330.1"
                     /translation="MTRIDFMQGNTACAEGALAAGCRFFGGYPITPSTEVAEHMAAKL
                     PKVGGVFIQMEDEIAGIASVIGASWTGARAMTATSGPGFSLMMENIGFAVMTETPCVL
                     VNIQRGGPSTGQPTMSAQGDMFQCRFGSHGDYSIIALSPGSVQEMYELTVKAFNLADR
                     FRVPTFLMADEIIGHMRERIEIPDSVPRVERKPLARGVLPFHADDDMVPGFPQFGMGH
                     HVHVTGLTHDERGYPCATSPSLHEDLVKRLVAKVESARHEIADYKVTNPDAEVVFITY
                     GSPSRTVDQVLHDRQAERIGHLRFRMVWPFPENALEQFPNAKVFLVPEQNLGLMAREI
                     ERHTCVPVVPLPRFGGALHTPAELEKAMEAWR"
     gene            complement(1938..2210)
                     /locus_tag="PKL26_01155"
     CDS             complement(1938..2210)
                     /locus_tag="PKL26_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS82331.1"
                     /translation="MRLVIDERRCKGCNLCTLVCPYRIFTPGTQPNARGIVVPVLDRP
                     ERCANCRLQALYGRTLCGMCQMICPDQAISWVEEKPGEARKVAIEF"
     gene            complement(2207..2764)
                     /locus_tag="PKL26_01160"
     CDS             complement(2207..2764)
                     /locus_tag="PKL26_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNS82332.1"
                     /translation="MDLCTPLSHEVLMLRAGDRVTLSGTVYTARDEAHLRMMEEGIPF
                     DPHGAVVYHCGPVIQGQRVIAAGPTTSARMNSLSGFLIEKGVRALVGKGGMGRQVVDQ
                     LKGRGVYLAFTGGCAALAASRMTLKGVFFEDLGMAEAVWAIELDRLPLVVGIDAAGND
                     LFEGVHRNAQAGFKKRFQARRDVPA"
     gene            complement(2770..3606)
                     /locus_tag="PKL26_01165"
     CDS             complement(2770..3606)
                     /locus_tag="PKL26_01165"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNS82333.1"
                     /translation="MIPSPGPALFAAVTKATCEAFRRAEITLPSDVIEALRNARESET
                     SEIARGELANILANLEMAERLSVPICQDTGVPVVYLTIPPHVPLTNGMYGAIAEGVRM
                     ATEEVPLRPNVVDPLTRENSGDNTGTGMPAIHVRPGDSLTITVLPKGAGAENMSRISM
                     MLPSEREQIPKFLAETMLIAGGKPCPPVILGVGIGGTFDEAASLAKEALLDPIDTMTP
                     YEQELLDAVNMLGIGPMGLGGSTTALAVKVKTAGCHTASLPVAVNVQCWACRRATVEV
                     PT"
     gene            complement(3800..4336)
                     /locus_tag="PKL26_01170"
     CDS             complement(3800..4336)
                     /locus_tag="PKL26_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNS82334.1"
                     /translation="MVRKPGKMYRNISKKAYTRREYMGGVPGSKVVQFDMGNLSGEFP
                     MEVSLAVEEACQIRHTALEAARITINRHLIKDLGRMNYRLKLRVYPHHVLRENKQATG
                     AGADRVSEGMRLSFGKAVGTAARVAPGQEIFTVYTIPQYLPQVKTALKSGGHKLPSPS
                     YINISERTVPESPAPQQA"
     gene            complement(4390..5154)
                     /locus_tag="PKL26_01175"
     CDS             complement(4390..5154)
                     /locus_tag="PKL26_01175"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS82335.1"
                     /translation="MTLVLKDLSKSFAGEGKTEVEALSSINLEVADGEFVSILGPSGC
                     GKTTLLRIIAGLDHATGGTILLDGNKIDGPCPRMAMIFQEYSLYPWRTVIDNIAFGLE
                     MKGMEKQKRREMAFQFLELVGLVDFAGSYPYELSGGMRQRVAVARALAIEPQILLMDE
                     PFGALDAQTRNMLQKELLEIWQKTRKTVIFVTHSVDEAVYLSDRVVVLTARPGTIKDM
                     VHIDLPRPRERTGAEFARIRRYVLDHVEKTPFSSKV"
     gene            complement(5192..>5936)
                     /locus_tag="PKL26_01180"
     CDS             complement(5192..>5936)
                     /locus_tag="PKL26_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82336.1"
                     /translation="LPILLVIGWEVASWVVNNPFIVPSLETVIPILLHPFSPEYTLGT
                     GSMVENALISIQRVGLGFLVAAVVAIPIGILMGRSAFLNDFFDSTIELLRPIPPLAWV
                     PLALAWFKIGLTSIVFIIFIGAFFPILLNTVDGVKSVKKTWLEVATTLGAKEQQIMTK
                     VVLPGAAPTIWTGLRVGFGIAWMCVVAAEWLPGISQGLGYLILYAYNFGQTNLIIAGM
                     VVIGVIGLLIDFVFKHVEKRWFSWRGLER"
BASE COUNT         1161 a         1806 c         1735 g         1234 t
ORIGIN      
        1 accgcagaga ggatgatgcc ctggccgccg aaccccgaga agcgcacctc gtgtctcatc
       61 gcctcacccc cattgcaggc ctgtccctcc ggacgatgtc gccgagaatg atcgcatctc
      121 ccggaattgc tttcccctct tctcggagcc ttgcggcttt ctgcaccagg attccatgag
      181 ccttgaaata ctcgacctgg tccatcacct gccggaactt gtttctcctc ccatagttgg
      241 tcgggcactg gaccagcgtc tcgatgaacg agaagccggg tatgccaagc ccttcatgga
      301 tcgaccgggt cagctccttg acgtggtagg aggtccagcg ggagacaaaa ttcgcaccgg
      361 ctgctgatgc gagttcgcaa aggtcgaacg ccggttcctg cgagccgtac ggcgtggtgg
      421 tggagagcgc tcctctcggg gtggtcgggc tcccctgccc cccggtcatc ccgtagatct
      481 ggttgttcat gcagaccacg gtgaggtcga tgttcctccg gcaggcgtgg atgaagtggt
      541 tgcctccgat ggcagcgagg tccccgtccc ctgtaaagac gacgacgtgc aggtcagggt
      601 ttgccatctt tatcccggtg gcaaaagcaa gcgccctccc atgggtggtg tggagggaat
      661 ctgtcatgat gtatcccggg gcacgggagg agcagccaat ccctgagaca aatatggtct
      721 cctccttttt ccagcccatc cggtccactg cgttcaaagt gcagtttatc acagtcccgt
      781 tcccgcatcc cgcgcagtaa atatgtggga gcctgtctgc ccggagccag tcatcatatg
      841 tcatcgccac gcctccatgg ccttctccag ttcagcggga gtgtggaggg cccccccgaa
      901 ccttgggaga gggaccaccg ggacacaggt atgccgctcg atctcccttg ccatcagccc
      961 gaggttctgc tcgggaacaa ggaagacctt cgcgttcggg aactgctcaa gcgcgttctc
     1021 tgggaacggc cagaccatgc ggaaccggag atggccaatc ctctccgcct ggcggtcatg
     1081 gagcacctgg tcaaccgtcc gggagggcga cccgtaggtg atgaagacca cctctgcatc
     1141 aggattagtg accttgtaat cggcaatctc gtggcgggcg gattcgacct ttgcaacaag
     1201 cctcttcacg aggtcctcat ggaggctcgg gctggtggca cagggatatc cccgctcgtc
     1261 gtgggtgagg cctgtcacgt ggacatggtg tcccatcccg aactgcggaa acccgggtac
     1321 catgtcgtca tccgcgtgga atgggagcac acctcttgcg agcggttttc gttctacccg
     1381 cggcacactg tccgggatct cgatccgctc ccgcatgtgc ccgatgatct cgtcggccat
     1441 gaggaatgtc gggaccctga aacggtccgc gaggttgaag gctttcaccg tcagctcgta
     1501 catctcctgg acgcttcctg gcgagagggc gatgatgctg taatccccgt gggacccgaa
     1561 acggcactgg aacatgtccc cctgcgctga catggtgggt tgccctgtag acggcccgcc
     1621 ccgctggatg ttgacgagga cgcagggggt ctctgtcatc accgcaaagc cgatattctc
     1681 catcatgagc gagaatccgg ggccgctcgt cgcggtcatc gcccttgcac cggtccatga
     1741 tgccccgatt accgaggcga tcccggcaat ctcgtcctcc atctggatga acacgccgcc
     1801 aaccttcggg agtttggccg ccatgtgctc ggcaacctcg gtcgatgggg tgatcggata
     1861 cccgccaaag aaacggcacc cggcggcgag cgccccctcc gcacatgccg tgttgccctg
     1921 catgaagtca attctggtca aaattcaatc gccaccttcc gggcctcgcc aggcttctcc
     1981 tcgacccagg agatcgcctg gtcagggcag atcatctggc acattccgca gagagtacgg
     2041 ccatagagcg cctgcaaacg gcagttcgca cagcgttccg gccggtccag caccggaaca
     2101 acgatccccc tggcattcgg ctgcgttcca ggggtaaata tccggtacgg gcagaccagt
     2161 gtacagaggt tgcacccctt gcaccttcgt tcatcgatta cgagtctcat gccggcacat
     2221 ccctccttgc ctggaacctc tttttgaacc cggcctgcgc attccggtga accccttcga
     2281 aaagatcgtt tccggccgcg tcgatcccca cgaccagcgg gaggcgatcg agctcgatag
     2341 cccacacggc ctctgccatg ccgaggtcct cgaagaagac tcctttcagg gtcatccggg
     2401 aggctgcaag tgcggcgcag cctccggtaa acgcgaggta caccccccgg cccttcagct
     2461 ggtctaccac ctgccggccc attcccccct ttcccaccag tgcgcggaca cccttctcaa
     2521 tcaggaatcc cgagagcgag ttcatccttg cggatgtggt gggccctgcg gcgatcaccc
     2581 gctgcccctg gataacaggg ccgcagtgat agaccactgc gccgtgggga tcaaagggga
     2641 tgccctcttc catcattcgg aggtgcgcct cgtcccttgc ggtatacact gtccccgaga
     2701 gcgtgacccg gtctccggca cggagcatca gcacctcgtg ggaaagcggg gtgcagaggt
     2761 ccatgacgtt caagtgggca cctccacggt tgccctgcgg cacgcccagc actggacatt
     2821 gaccgcaacg ggaagagacg ccgtgtggca ccccgcggtc ttcaccttga ccgcaagcgc
     2881 cgtggtactc cccccgaggc ccatcgggcc tatgcccagc atgttgaccg catcaagtag
     2941 ttcctgctca tagggagtca tcgtgtctat gggatcgagc agtgcctcct tggcgagcga
     3001 cgcagcctca tcgaacgttc ccccgatccc cacgccaagg atgaccgggg ggcagggctt
     3061 cccaccggcg atcagcattg tctctgccaa gaacttcggg atctgttccc gctctgatgg
     3121 gagcatcatg gagatccttg acatgttctc ggcaccggcg cccttgggca gcacggtgat
     3181 cgtaaggcta tcgccgggcc tgacgtgaat ggcaggcatc cctgtcccgg tattatcccc
     3241 ggaattttcc cgcgtgaggg gatcgaccac gttcggccgg agcggtacct cttcagtcgc
     3301 catcctcacc ccttctgcga tcgccccgta catcccgttt gtgagcggga cgtggggagg
     3361 gatggtgaga tatacgaccg gaacccccgt gtcctggcag atggggacag agagtctctc
     3421 tgccatctcc aggttggcaa ggatgttcgc gagttccccc cttgcgatct cgcttgtctc
     3481 gctctccctt gcattgcgga gtgcctctat cacgtccgag ggaagggtaa tctctgcccg
     3541 ccggaatgcc tcgcaggttg cttttgtgac ggcagcgaag agggcggggc caggggatgg
     3601 gatcatgctg ataaaaggtt tggagtgaat gggaatataa attgtgataa ggcccgaaca
     3661 ggggacattc ccttcggcag gttccccctc gtatttcatt acctggcagg cacctggatt
     3721 ggagatcttt ttgattgcac agccagaacc ggcatagata aaggggaaaa aacctggggt
     3781 aaatagccaa aaaaggggtt tatgcctgct ggggtgcagg ggactcggga acagtcctct
     3841 cggatatgtt gatgtacgag ggggagggaa gtttgtggcc gcccgacttg agtgcggtct
     3901 tcacctgggg aaggtactgg ggaatggtat agacggtgaa tatctcctgt ccgggtgcga
     3961 ctcgtgcggc agtcccgact gccttcccaa acgagagacg catcccctcg gatacacggt
     4021 ctgcccctgc cccggttgcc tgcttgttct cgcgaagtac gtgatgcggg tacacacgga
     4081 gcttcaaccg gtagttcatc ctcccaaggt ccttgataag gtgccggttg atggtgatac
     4141 gtgctgcctc gagcgcggtg tgacgaatct ggcaggcctc ctctaccgcg aggctgacct
     4201 ccatcgggaa ctccccggac agattcccca tgtcgaactg aactactttg ctgccgggaa
     4261 ccccgcccat gtactcccgg cgggtatacg cttttttcga gatgttccgg tacatctttc
     4321 ctggttttcg taccatgact gaatactcct tatccctgtc tgtgctttat aaaatgggga
     4381 tatatcttct taaaccttac tggaaaacgg agttttctcc acgtgatcga ggacataacg
     4441 tcggatcctt gcaaattctg cgcctgtcct ctcccttggg cgcgggagat cgatatggac
     4501 catgtctttt atggtcccgg ggcgggcggt gagcaccacc acgcgatcag agagatatac
     4561 agcttcgtcc acgctgtggg tgacaaagat gacggtcttc ctggttttct gccagatctc
     4621 aagaagttcc ttctggagca tgttccttgt ctgggcgtcc agtgccccga acggctcgtc
     4681 catcaggagg atctgcggtt cgatggcgag ggcccgtgct accgcgaccc gctggcgcat
     4741 tccgccggag agctcgtacg ggtaacttcc ggcaaaatcg acgagaccga caagttccag
     4801 gaactgaaaa gccatctccc tgcgcttctg cttctccatt cccttcatct cgaggccgaa
     4861 cgcgatgttg tcgatcaccg tccgccacgg atagagggag tattcctgga atatcatggc
     4921 catccggggg cagggaccat cgatcttatt cccatcgaga aggatcgtcc cgccagttgc
     4981 atggtcaagc ccggcaatga tgcggagaag ggtagtcttc ccgcatccgg aagggccgag
     5041 gatcgagacg aactcgccgt ccgccacctc aaggttgatg ctggagagtg cttccacctc
     5101 tgtcttccct tcacctgcga aggattttga gagatccttg agcaccaggg tcacaggtat
     5161 tcaccacccg gcaaggtttt tcggacccgg ctcatcgctc gagccccctc cacgagaacc
     5221 atctcttctc gacgtgcttg aagacaaagt cgatcaggag gccgattacg ccgatcacga
     5281 ccatcccggc aatgatcagg ttggtctggc caaagttgta ggcatagagg ataagatacc
     5341 cgagcccctg cgagatgccc ggcagccact ccgcggcgac gacacacatc caggcgatcc
     5401 cgaaccccac gcgaaggccg gtccagatgg tgggggcggc gccggggagc acgacctttg
     5461 tcatgatctg ctgttccttc gccccaagcg tggtcgcgac ctcgagccat gtcttcttca
     5521 ccgatttcac gccatcgaca gtgttcagga gtatcgggaa gaatgcacct atgaagatga
     5581 tgaagacaat ggaagtgagc ccgatcttga accaggcgag tgcgagaggg acccaggcaa
     5641 gtgggggaat tggcctgagc agctcgattg ttgaatcgaa gaagtcgttg aggaatgccg
     5701 accttcccat caggatgccg atcggtatcg ccacgactgc ggcgacaagg aaaccaaggc
     5761 ccacgcgctg gatactgata agtgcattct ccaccatgct cccggtccca agcgtatatt
     5821 cggggctgaa cgggtgcagg aggatgggga ttacggtctc aagggacggg acgataaagg
     5881 gattgttgac aacccacgac gcaacctccc acccaatgac aaggagaatg gggagg
//
LOCUS       DAOQVJ010000030         5842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_15092_length_5842_cov_4.677899, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000030 DAOQVJ010000000
VERSION     DAOQVJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15092_length_5842_cov_4.677899"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKL26_01185"
     CDS             complement(<1..194)
                     /locus_tag="PKL26_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82337.1"
                     /translation="MSDDILVKDIMSKPVTISKSALITDALDRMLDEGIDPLIVVNHN
                     TVVGTVSRQSVADKLGTKRNS"
     gene            complement(191..1030)
                     /locus_tag="PKL26_01190"
     CDS             complement(191..1030)
                     /locus_tag="PKL26_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82338.1"
                     /translation="MQAQDIMTSPVYVVNPGEHVAYARNLMLKHKISRVPVMDAGALA
                     GILTKKDIGYRLQQSEPIWRRRPIDKIPVSILMTRDPLTISPTTTIREIAALMIHWDI
                     SGLPVVDEGEVGGIVTKSDFMKSATINSLSQTVADVMEDAISVSRYHSLDHIVDIMKE
                     RNDKVVVVNNDGTLAGIITESNLAFYLYETERRESPRKDVTMLRRGESAGRKQYRHVI
                     EMAAVAEDLMSRPVITVDGNAKLTEAIEIMQANHINSVVAVEGNEIKGILKRDDIIRE
                     VAK"
     gene            complement(1037..1930)
                     /locus_tag="PKL26_01195"
     CDS             complement(1037..1930)
                     /locus_tag="PKL26_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82339.1"
                     /translation="MPGKLDRGPIEFKSRVVEQEGEIMAIATGDVVSVPQTMSIIGAV
                     ETMTRCGFRRLPVVDAGSRKIRGIVTSGDIINFLGGGDKFNLVQVKHDGNLLSAVNES
                     IRSIMTTQVTSLPDSASLRDALDIIMGKMIGGIPIIDQDEALVGIVTERDVMTVLANE
                     RVSCTVEEIMSRSLRVTEPDCPIGKVTKEMTRYRFRRLPVVSDEVLYGIITTTDIMKY
                     LGSKRVFDQLETGDIAEVMALPVRTLVSGNLYTIAPDRSVNQAAAEMVKRNAGALPVI
                     EDARLIGLVTEFDLVKALSRG"
     gene            complement(2056..2808)
                     /locus_tag="PKL26_01200"
     CDS             complement(2056..2808)
                     /locus_tag="PKL26_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82340.1"
                     /translation="MKIARDFMVRVPLLKYDEKATRARQVLRDDVYRELYVHDGRRRL
                     MGCVDISGVLNVTATRSNVTIEGFIKEVSPVGPDSSIDTVAKTIRENGTDSIPVVDEG
                     GQVLGGVLLSEIFPVIITRHRLGGLVEEYMSRKVVECPADEPIHRIHTLITESGFSAF
                     PVVKKKRLVGIISRRDLLRDGRWRVSAEGASSTPVSGVMTTPVITVSPGESVQKAAEM
                     LVRHDISRLPVVLGDELIGIIDRHDVLNAIPD"
     gene            complement(2805..3374)
                     /locus_tag="PKL26_01205"
     CDS             complement(2805..3374)
                     /locus_tag="PKL26_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82341.1"
                     /translation="MKKSSDTIRFDTRVPVREVMRRNPTTIGYEGTVARAAMLMCRDE
                     VGSCIVLGRDNLPVGIVTEEDINCKVVAKDLKPSAVQASEIMSTPLITISADKYAKDA
                     AHMMIRHRVRRLPVVDDENKVIGIVTVRDILTVSTEINELMNDLVEINRLEEVEVGTC
                     SRCGQMSDDLRRTDNLMLCPSCREEELLQ"
     gene            3585..4226
                     /locus_tag="PKL26_01210"
     CDS             3585..4226
                     /locus_tag="PKL26_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HNS82342.1"
                     /translation="MNILVQTKRRLSKFLMAFFKKKKARIGIYGPPNAGKTTLANRIA
                     RDWTGEDVGLVSEVPHETRRAVRKSDISITGSNGSNITIDIVDTPGVTTKIDYKDFLE
                     YGMDKETAVIRAREATEGVAEAMHWLREDINGVMYMLDATEDPFMQVNIMMIGIIESR
                     KLPVIIVANKIDLPDASPQRIRSAFPQHPVVPISCLEGKNIEDLYEKMVEHFG"
     gene            4233..4598
                     /locus_tag="PKL26_01215"
     CDS             4233..4598
                     /locus_tag="PKL26_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HNS82343.1"
                     /translation="MMQGIQIDLISSEKLAALTSMEKIHLILDHVRKGSIIILEKGLS
                     PDEQSTLIEMTMREILPDGFNGIEIETYPSRDQSPGFLQKLLGRSAIESRLTVIGPAN
                     QLRMIKKEKDIISAWVSTR"
     gene            4605..4985
                     /locus_tag="PKL26_01220"
     CDS             4605..4985
                     /locus_tag="PKL26_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HNS82344.1"
                     /translation="MPHRCVNCGREYRTNECEILKGCRECGGKKFIFVPLVEREKEAK
                     APDLSEGKEEKVQREEEPAGIARLPGHEPLESIRIVSPGTYELNIEKMASSDERVVGV
                     GKGEGSYLVDLLSMVKPKKKKSKK"
     gene            complement(5002..5307)
                     /gene="yciH"
                     /locus_tag="PKL26_01225"
     CDS             complement(5002..5307)
                     /gene="yciH"
                     /locus_tag="PKL26_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNS82345.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRISVKVDRRRYGKEVTVI
                     EGLNPTDIDLEDLSKYMKGRLACGGTVKGTSIELQGNHKERVKDLLVHKGYSVDNIN"
     gene            complement(5404..>5842)
                     /locus_tag="PKL26_01230"
     CDS             complement(5404..>5842)
                     /locus_tag="PKL26_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNS82346.1"
                     /translation="GIEPLFACAGERMIDKKAFRIMHPVLHTILRDLPRGRDLLNEVE
                     KTGSVQHLAVPEEIHELFRNAAEIDPGHHVRMQAAFQKHVDNAVSKTVNLSELTTPED
                     ISRIFLLARELGCKGVTVYRYRSRKDQVLSRGCDTCRVDSLLL"
BASE COUNT         1250 a         1686 c         1522 g         1384 t
ORIGIN      
        1 gagttcctct tcgtcccgag cttatctgcc acgctttgcc gtgaaacagt gcctactacc
       61 gtgttgtggt tcaccacgat gagagggtca atgccttcgt caagcatcct gtccagggca
      121 tcggtgatga gtgcggactt ggagatggtg accggcttgg acatgatgtc ctttaccaga
      181 atatcgtcgc tcattttgcc acttcccgta taatatcgtc tctttttaaa attcctttga
      241 tctcgttgcc ttccactgct accacgctgt tgatgtggtt tgcctgcatt atctcgatgg
      301 cttctgtcag tttcgcattc ccgtcaacgg taatgacagg gcgggacatc aggtcttccg
      361 cgacagccgc catctcgatc acatgccggt actgcttcct cccggccgat tccccacggc
      421 gcagcatcgt cacgtctttt cttggcgatt ccctcctttc tgtctcgtag aggtagaatg
      481 caaggttgct ttccgtgatg atccccgcca gtgtgccatc attattgacc acgactacct
      541 tatcattcct ttccttcatg atgtcgacaa tatggtcaag agagtggtac ctgctgacag
      601 aaatagcatc ttccatgaca tcggcaacag tctgtgagag agagttgatc gttgcagact
      661 tcatgaagtc cgacttcgta acaattcccc ccacctctcc ttcatctacc actggaaggc
      721 cgctgatatc ccaatggatc atcagggctg caatctcgcg aatcgtagta gtgggggaga
      781 tagtgagggg atcgcgggtc atcaggatac tcacagggat tttatcgatt ggccgacgcc
      841 tccagatagg ttcgctctgt tgcagccggt acccgatgtc cttcttggtg aggatgcccg
      901 caagtgctcc tgcatccatg accggcaccc tggagatctt gtgcttcagc atcagattgc
      961 gtgcgtacgc gacgtgttct cctgggttga ccacatagac cggagatgtc atgatatctt
     1021 gtgcctgcat tacgaatcac ccccttgaga gggctttgac caggtcaaac tcggtcacga
     1081 gcccaatcag cctcgcatct tcgattaccg gaagcgcgcc cgcgttcctc ttcaccattt
     1141 ctgcggcagc ctggttcacg ctccggtcag gggcaatagt gtagaggttc ccggagacca
     1201 gtgtccggac cggcaacgcc atcacctcgg cgatatcccc cgtctccagc tggtcgaata
     1261 cacgcttgct tccgaggtac ttcatgatat ccgtcgtcgt gatgatgccg tagagcacct
     1321 cgtcgctcac caccggcagc ctgcggaacc ggtacctggt catctccttt gtgactttcc
     1381 cgatcgggca gtccggttcg gtgaccctga gggaccggct catgatctct tccacggtgc
     1441 aggataccct ctcgtttgca agcacggtca tgacatcgcg ctctgtcacg atgcccacca
     1501 gcgcctcatc ctgatcgatg atggggatgc cccctatcat ctttcccatg atgatatcga
     1561 gggcatcccg gagcgatgcg gagtcgggaa gcgatgtcac ctgggtcgtc atgatgcttc
     1621 ggatgctctc gttgacggcc gagagcaggt tcccgtcatg cttcacctgg acaaggttga
     1681 acttgtcccc acctcccagg aaattgataa tatcccccga agtgacaatc ccacggattt
     1741 tcctcgagcc ggcatccacc acgggcagcc tccggaaccc gcaccgggtc attgtctcca
     1801 ccgccccgat gatgctcatg gtctgcggca cggataccac gtcaccggtc gcgatcgcca
     1861 tgatctcccc ctcctgctcg acgaccctcg atttgaattc gatcggcccg cgatcgagtt
     1921 ttcctggcat tttcagaagt ttatcgctct gtttatagtt cctgtcaatg tgatgtacca
     1981 ttcctgcccc cgaaaatgta ttgctcgaat atcccggtcc atgatgaact ccgggattgc
     2041 tcgtccttcc ccaatttaat ccggaatcgc attcagcaca tcgtggcggt cgatgatgcc
     2101 gatcagttcg tctccgagaa ccacggggag acggctgatg tcatgcctga ccagcatctc
     2161 cgcggccttc tgcacgctct ccccgggaga gacggtgatc acgggtgtgg tcatcactcc
     2221 agaaacaggg gtactcgacg ccccctctgc agacaccctc caccgtccgt ctctcagcag
     2281 gtcccggcgg gagattatgc ccaccaggcg cttcttcttc accaccggaa atgcggaaaa
     2341 cccgctttca gtgatcaggg tgtggatccg gtgaatgggc tcgtcggccg ggcattccac
     2401 gaccttcctt gacatgtact cttccacaag tcccccgagt ctgtgccggg tgatgatcac
     2461 agggaaaatt tctgaaagga gcacccctcc aagtacctgg cctccttcat caactaccgg
     2521 tatactgtcg gtcccgttct cccgtatcgt ttttgcaacg gtatctatgg acgaatccgg
     2581 gccaacagga ctgacctcct tgataaagcc ctctattgtc acgttagaac gcgttgccgt
     2641 gacgttcagt acccctgata tgtccacgca ccccatgagc cggcgtctcc cgtcatgcac
     2701 atagagttcg cgatacacat cgtcacggag cacctgcctc gcccgggtag ctttctcgtc
     2761 atatttcagg aggggtaccc ttaccatgaa gtcccgggca attttcattg tagcagttcc
     2821 tcctcacggc aagacggaca gagcatgagg ttgtctgtcc gcctgagatc atctgacatc
     2881 tggccgcacc tgctgcaggt cccaacctcc acctcttcca ggcggttgat ctccaccagg
     2941 tcgttcatca gctcattgat ctctgtggat accgtgagaa tatcacgaac tgttacaatt
     3001 ccaattacct tgttctcgtc gtcaaccaca gggagccgcc ggacccggtg cctgatcatc
     3061 atgtgcgctg catccttcgc atacttgtct gcgctgatgg tgatgagcgg agtgctcatg
     3121 atctcgctgg cctggacggc acttggcttg agatcctttg ccaccacctt gcagttgata
     3181 tcctcctcgg tcacgatccc gacggggagg ttatccctgc caagcacaat gcagcttccc
     3241 acctcatccc ggcacatgag catagccgca cgggcaaccg tcccttcata tccgatggtg
     3301 gttgggttgc gccgcatgac ctcccgtact ggtacacggg tatcgaagcg gatggtatcg
     3361 gatgatttct tcaatcgatc acctccatgt tgttgacaca agtgggttta ttgcagttac
     3421 caatacggca aatggagtct atatactttc tgcaagttcc agtgcgccca gaaacgcaag
     3481 gataataaat ctaataacca ccccccccta aaaggatagt aacggattcg catgacccgg
     3541 gggatatact gcacattccc tgcctggtca tgacgaggtg tacaatgaat atcctggtcc
     3601 agaccaagag acgtctctca aagtttttaa tggctttctt caagaagaaa aaggccagaa
     3661 ttggaatata cggcccgcca aatgcaggaa agaccacact cgccaaccgt attgccaggg
     3721 actggaccgg ggaggacgtg ggcctggtaa gcgaggtccc ccatgagacc cggcgggcgg
     3781 tgcgaaagag tgatatcagc atcaccggat caaatggaag caacatcacc atcgatatcg
     3841 tcgatactcc cggggtcacc accaagatcg actacaagga ctttcttgaa tacggcatgg
     3901 acaaagagac tgcggtgata cgcgcgaggg aagccactga aggggtcgcg gaagcaatgc
     3961 actggctccg tgaggatatc aacggtgtca tgtacatgct ggatgcgaca gaggatccct
     4021 tcatgcaggt caacatcatg atgatcggga tcatcgagag ccgcaagctg ccggtgatca
     4081 ttgtcgcaaa caagattgac ctgcccgacg cgtcgccgca gcgtatccgc agtgcgtttc
     4141 cccagcatcc tgtggtaccg atatcctgcc ttgaggggaa gaacatcgag gatctgtacg
     4201 aaaagatggt ggagcatttt ggataggggt ggatcatgca gggaatccag atagatctga
     4261 tatcatccga gaaactcgcg gcgctgactt cgatggagaa gatccatctc atcctcgacc
     4321 acgtgcggaa gggctccatt atcatcttag agaagggcct ctctcctgac gagcagagca
     4381 ccctgatcga gatgaccatg cgcgagatcc ttccggacgg gttcaacggg atagagatag
     4441 agacctaccc ctccagggac cagagtcccg gttttctcca gaaactgctg ggaagatcag
     4501 cgattgagtc gcgtttgacc gtcattggcc cggcaaacca gctgcggatg ataaaaaaag
     4561 agaaggacat catcagcgcc tgggtctcca cgcggtgagt tctcatgcca caccggtgcg
     4621 tcaactgcgg gcgggaatac cgcaccaatg agtgcgagat cctgaaaggc tgcagggaat
     4681 gcggtggcaa gaagttcatc tttgtgcccc tcgtcgagag ggagaaggag gcaaaagccc
     4741 cggatctttc agaaggaaag gaggagaagg tgcagcgtga ggaggagcca gccggtattg
     4801 cgcgcctccc gggccacgaa ccactggaga gcatccgcat cgtctctcct ggcacatacg
     4861 agctgaatat cgagaagatg gccagctccg acgagcgtgt cgtcggagtc gggaaagggg
     4921 agggcagcta cctggtggac ctcctttcaa tggtcaagcc aaaaaagaaa aagagcaaga
     4981 aataggtttc ttatttcatt ttcagttgat attatccacg ctgtatccct tgtgcaccag
     5041 caggtccttg accctctcct tgtgattgcc ctgcagttcg atggaggtgc ccttcaccgt
     5101 tccgccgcac gcaagtctcc ctttcatgta cttggagagg tcttccaggt cgatgtccgt
     5161 ggggttcaag ccctcgatca cggtgacttc tttcccgtaa cgtctcctgt cgaccttcac
     5221 gctgatacgc tgctgttctt ttgcgacttc ttcacagatg cataactctt tcggcagtcc
     5281 acacgtcgga catatcccac cattcattac aagtaccttc aagttctgct tatatatttg
     5341 ttggcatccc cagaatcgcg caatgcacgt ataacgatgg aaatccgggt ctttttcgaa
     5401 gtctcacagg agaagcgagt ccaccctgca ggtatcgcac ccccgtgaca ggacctggtc
     5461 cttccggctg cggtagcgat agactgtgac tcccttgcag cccagttccc tggcgaggag
     5521 gaaaatgcgg gagatgtcct cgggggtggt aagttcggac aggttcacgg tcttggagac
     5581 ggcattgtca acgtgcttct ggaaggcggc ctgcatcctc acatgatgcc cgggatcgat
     5641 ctctgcggcg ttcctgaaga gttcgtggat ttcctccggt actgcgaggt gctggacact
     5701 ccccgtcttt tccacttcat tcaggagatc ccttccccgg ggaagatcgc ggaggattgt
     5761 atgaaggaca gggtgcatga tgcgaaaggc ttttttgtcg atcatcctct ctccggcgca
     5821 tgcgaagagc ggctcgatcc cg
//
LOCUS       DAOQVJ010000031         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_15232_length_5805_cov_3.930957, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000031 DAOQVJ010000000
VERSION     DAOQVJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15232_length_5805_cov_3.930957"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKL26_01235"
     CDS             complement(<1..1013)
                     /locus_tag="PKL26_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82347.1"
                     /translation="MRMVHPGNLKNRNLTFLLVSLIFLVLLPGIAVAATNDTATCTIL
                     SVLTGDSHQCYPAIHDDKVIWIDSPGSGCAHLYNLSTGEECVLTTAFIPADGLCPDVS
                     CRYATWTTFDGSGYGVILHDMQTSEESWIAGGNGSTALNAKISSDCVVWESNALGRFD
                     IYQMDIRTGEVILLTPGTDDSDQTNAVVSGDWVAWQSLDPTSGTNDIYLASLLTGNVT
                     LVTPGTEDTDELYPAIDGDHLVYQRLDPANYCYDIYLYALSSKETVLLTPDTDSTSEE
                     YPAVHQGKVIWLGQDPGDFSYQLYIYETGSGLTYRLQRDFDTDSPGLPRIFGNRIVWQ
                     QPNP"
     gene            complement(1047..1859)
                     /locus_tag="PKL26_01240"
     CDS             complement(1047..1859)
                     /locus_tag="PKL26_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNS82348.1"
                     /translation="MTATACSKTPYPEWNDPLLGVNYGNQNQGPCCFIASLSAIAWIW
                     PYNVEPFKCRSPFSTEADGTRKYKIPFKFPPENIEINEKVALEGSNYCFSRSMDAQEL
                     WVSLYEKAYARKLTQADMCDSYASVPWPGNAIDPLKHITGYSANSEIDSTKIYSKIQS
                     KCMSYKTKYATVVWSSSHAYSVLGYMPYNSTEYLVLRDPAGSNLYIRSDIVENVDWSI
                     PEDFVWNPFTNTKSNSPCRGGSLKIRLTGGIFGLKYDKVKDYFQGCAWAGPV"
     gene            2235..2933
                     /locus_tag="PKL26_01245"
     CDS             2235..2933
                     /locus_tag="PKL26_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS82349.1"
                     /translation="MTILIAIDDTDTKESRGTGRLARTIADSLRHFGSVAGVTRHQLF
                     VHPSIPYTSHNSCAVIHLQTANGVAVPEIVDFVSGKILDDFIEGSDPGLAVAPTSGIG
                     ELVVKFGQDAKKCVLSRRDAFTLAERRGIALAGLGGSCDGVIGALAGLGLASSGNDGR
                     YVMKGRLREFSREARVDDLLLAGVDEVHTMSGERVVFGTVRMRKFPKPSLRNHRAVLF
                     VEERDGSFDEVVRD"
     gene            complement(2968..3885)
                     /gene="argF"
                     /locus_tag="PKL26_01250"
     CDS             complement(2968..3885)
                     /gene="argF"
                     /locus_tag="PKL26_01250"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNS82350.1"
                     /translation="MKRDIISILDVTAGELGKLLDEADRLKQMRKSGIPHPLLPGKAL
                     AMIFEKSSTRTRISFEVGMADLGGSALFLNAQDMQIGRGEEIRDTARVASRYVSGVMI
                     RAYRHDTIREFARYSSVPVINGLSDLEHPCQILADIMTIREHFPDLAELRLGWAGDGN
                     NVCNSLILSTVLTGMEITVATPEGFEPPSHVVDAARRKGGRVTLVRDPRDATRDAHVV
                     ATDTWVSMGTEKEREARLKAFSGFTIDRNLMDIASPDAIFMHCLPAHRGQEVTDEMIE
                     GPRSVVFDEAENRLHAQKALLVFLMQDGE"
     gene            complement(3882..5174)
                     /gene="purD"
                     /locus_tag="PKL26_01255"
     CDS             complement(3882..5174)
                     /gene="purD"
                     /locus_tag="PKL26_01255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNS82351.1"
                     /translation="MTMKILVVGGGGREHAISRALSRNNETELYAVMARENPGISRLC
                     RDFLLEKETRVDSVSAFAKKKSVDYAIIGPEAPLEAGVVDRLEADGIPCMGPTRAAAR
                     LETDKAFCRVMMEQHGIAGCPRYRVFRAATDAVGFLEEYDGDLAIKPAGLTGGKGVRI
                     MGEHLDRKGAIEYIMSLGSDIVLEEKLVGEEFTLQAFVDGTHLVPMPLVQDHKRAFEG
                     DEGPNTGGMGSYSMPDHGLPFVTGIDRRKAFGIMQAAVSAMEEAGTPYRGILYGQFMN
                     TRDGPMVIEFNARFGDPEAMNVLSLLESDLLDIIERSIDGTLSRGRVRFLEQATVCKY
                     LVPDGYPESPIMGQPVSLGPYEPALLYYANVEERGGTLYTQSSRTMAFVGMAASLAGA
                     EEIAERAAEGVKGKVRHRRDIGSPALLKKRVDHMRELR"
     gene            complement(5194..5724)
                     /gene="pyrE"
                     /locus_tag="PKL26_01260"
     CDS             complement(5194..5724)
                     /gene="pyrE"
                     /locus_tag="PKL26_01260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNS82352.1"
                     /translation="MVNRIAHLMEEYGALEFGDFVLASGARSSYYLDIKKVVTQPAAL
                     KEIAGAIARDYAFEVVAGVAVGGVPLAVAVSLESMKPFAIVRSQEKHHGKAGRIIGDV
                     RGKHVLLVEDVTTSGGSALFGICALRDAGATVNRVVTVVDRESGATALLHENGVALDA
                     LVRASDVIGRREEQQS"
BASE COUNT         1240 a         1696 c         1532 g         1337 t
ORIGIN      
        1 ggattgggct gttgccagac gatcctgttt ccaaagatcc gcggcagacc gggggagtct
       61 gtgtcgaaat ccctctggag ccggtatgtc agaccagacc ctgtttcgta aatatagagt
      121 tgatacgaga agtccccggg gtcctgtccc agccatatca ccttcccttg atgaaccgcc
      181 ggatactcct cgcttgtaga atctgtatca ggagtcagga gcacagtctc tttagaagag
      241 agtgcataga gataaatatc gtaacagtag ttggccgggt ccagtcgctg gtaaacaaga
      301 tgatcgccat cgatcgctgg atagagttcg tcggtgtctt cagttccagg ggtaacgagt
      361 gtcacatttc ccgtcaagag tgatgcgagg tagatatcat ttgtacctga tgtaggatcc
      421 aggctctgcc atgccaccca atcacctgaa actacagcat ttgtctggtc actgtcatct
      481 gttcccggtg tgagcagtat aacttcgcct gtgcgaatat ccatctggta gatatcaaat
      541 cttccgagag cattcgattc ccataccacg cagtcactac tgatcttggc attcagggca
      601 gtggatccgt ttccgccggc aatccaggat tcctctgatg tctgcatatc atggaggatt
      661 accccatagc ccgatccgtc aaaggttgtc caggttgcgt acctgcagga tacatcggga
      721 cagagcccgt ccgccgggat aaaggcagtg gtgagaacac actcctctcc tgttgagaga
      781 ttatagagat gggcacagcc tgacccaggg ctatcgatcc agataacctt gtcatcatgg
      841 atggccgggt aacactggtg tgaatcccct gtgagcacag agaggatagt gcaggtggcg
      901 gtgtcattcg ttgcagcaac cgcaatccct ggtagaagaa cgagaaatat gagtgatacc
      961 aggagaaagg taagattccg attcttcagg ttaccagggt gcaccatccg catgatccac
     1021 gtcctcctca ctggatcatc agggcatcac accgggccag cccatgcaca gccctggaaa
     1081 tagtccttga ctttatcata cttcaggccg aaaattcctc ccgtgagtct tatcttcaaa
     1141 ctgcctcccc ggcagggaga gttcgacttg gtattggtaa agggattcca tacaaaatcc
     1201 tcaggtattg accagtcaac gttttcgacg atatcagacc tgatatataa atttgagcct
     1261 gcgggatccc tcagaaccag gtattcagtc gaattatacg gcatataacc caaaactgaa
     1321 tatgcatggc tactactcca gacaaccgtg gcatatttgg tcttgtaact catgcacttt
     1381 gactggatct tggaatatat tttggtcgag tcgatttcac tattcgcaga atatcccgtg
     1441 atatgtttca ggggatcaat tgcattcccg ggccacggaa cacttgcgta ggagtcgcac
     1501 atgtccgcct gcgtcaattt tcgtgcgtat gccttctcat agagggacac ccacaactcc
     1561 tgcgcatcca ttgatcgtga gaagcagtaa ttggagccct ctaatgccac cttttcgttg
     1621 atttcgatgt tttcgggggg aaacttgaag gggatcttat atttccttgt tccatctgcc
     1681 tccgtgctaa agggggatct gcatttgaat ggctcgacat tgtagggcca tatccacgca
     1741 atcgctgaga gagatgcgat gaagcagcat gggccctggt tctgattgcc gtaattgaca
     1801 cccagcaatg gatcattcca ttcgggatac ggggttttac tgcatgctgt tgcagtcatc
     1861 ttacagaatt cgctacctga tactttctta cattcacatg ccatgaaaag cctccaaatc
     1921 gaacagatta accagacgcc gcaagatgct gaatgtgggc atccatcctc ccactgcaaa
     1981 ccatgtgcac ccccaacccc caagctggac gagtaccgaa cgctgtactc ctggcgttac
     2041 atcatactca tatcacttat ttaaaaagct tatgcgagga ttatcttcaa taattaaaaa
     2101 aaacaacaaa cacctcgctc aatatcggca accaggtgac aggcgaatcc actcaagagt
     2161 gtgcagcctt ctacacaatg ttattccctt ttccggatca tacaatacag ttactttacc
     2221 tcccgtgacc ttccatgact attctcattg ccatcgatga cactgataca aaggagagcc
     2281 gtggaacagg caggctcgcc cggactatcg ccgattcact ccgccatttt ggttcagttg
     2341 cgggcgtgac caggcaccag ctcttcgtgc atccgtctat cccatatacc tcgcataaca
     2401 gttgtgcggt gattcacctt cagaccgcaa acggagtcgc tgttcccgag atcgtggatt
     2461 ttgtgagcgg gaagatcctc gatgatttta tcgaggggag cgaccccggc ctcgccgtgg
     2521 cacccacttc cgggattggg gaactggtgg tgaaattcgg gcaggacgca aaaaagtgcg
     2581 ttctctcccg cagggatgca ttcacccttg ccgagaggag ggggattgcc ctggccggtc
     2641 ttggcgggag ctgcgatggc gtcatcgggg ctcttgcagg gcttggcctg gcttcgtccg
     2701 ggaacgatgg caggtacgtg atgaaaggga ggctgcgtga gttttcccgg gaagcgagag
     2761 tggacgatct tcttttggcc ggtgtggacg aggtgcatac catgtcgggc gagcgcgtgg
     2821 tgttcggcac ggtccgaatg aggaaatttc caaagcccag cctccgcaac cacagggcag
     2881 tcctcttcgt tgaggagcgg gacggctcct tcgacgaggt agtaagggac taaaaaaaat
     2941 tgccccggtt ctcctgccgg ccccccgtta ttccccgtcc tgcatcagga acacgagcag
     3001 ggctttctgg gcgtgaaggc ggttctccgc ctcgtcaaag accacgctcc gcggcccctc
     3061 gatcatctcg tccgtgacct cctggccgcg atgggccggc aggcagtgca tgaagatcgc
     3121 atccggcgat gcgatgtcca tcagattgcg gtcaatggta aatccgctga atgccttcag
     3181 cctggcctcc cgctccttct cagtgcccat ggagacccag gtgtcggttg ccaccacgtg
     3241 tgcatcccgg gttgcgtccc gcgggtccct gaccagggtg accctccctc ctttccttcg
     3301 tgccgcatcc accacgtgcg agggtggttc aaacccttca ggggtggcca cggtgatctc
     3361 catgccggtg aggaccgtcg agagtattag cgagttgcag acattattcc cgtctccggc
     3421 ccacccgagg cgcaactccg cgagatccgg aaagtgctcc cggatggtca tgatgtcggc
     3481 aagtatctgg cacgggtgtt cgagatcaga gaggccgtta atcacgggga cagaggaata
     3541 ccgggcgaac tccctgatgg tgtcgtgccg ataggccctg atcatcaccc ccgacacgta
     3601 cctcgatgcc accctcgcgg tatcacgtat ctcctccccc ctcccgatct gcatgtcctg
     3661 ggcgttgagg aagagggcgg atcccccgag gtctgccatc cccacctcaa acgagatccg
     3721 ggtccgggtg gaggacttct caaagatcat tgcaagggcc tttcccggga ggagtggatg
     3781 gggaatcccg gacttccgca tctgtttcag ccggtctgcc tcatccagca gcttcccgag
     3841 ttcccctgcc gtcacgtcca gaatagagat gatatcgcgt ttcatcgaag ttccctcatg
     3901 tgatccacgc gtttcttcag cagggctgga gaaccgatgt ccctgcggtg acgaaccttc
     3961 cccttcactc cctctgcggc acgctcggcg atctcctctg cacccgcgag ggaggctgcc
     4021 atccccacaa acgccatggt ccgggaggac tgggtataga gcgtgccgcc tctctcctcc
     4081 acgttggcgt aatacaggag cgccggttcg tatggtccga gcgagacagg ctggcccatg
     4141 atcggggatt cgggatatcc atctggcaca aggtacttgc agacggtggc ctgttccagg
     4201 aaccggaccc tgcctcggga aagggtcccg tctatgctcc tctcgatgat atccaggaga
     4261 tcgctctcaa ggagcgagag gacgttcatc gcctcagggt ctccgaacct ggcattgaac
     4321 tctattacca tcggaccatc gcgggtattc atgaactgcc cgtagagaat cccgcggtac
     4381 ggtgttccgg cctcttccat ggccgaaacc gcagcctgca tgatcccaaa cgccttgcgc
     4441 cggtcgatcc cggtcacaaa ggggagccca tggtctggca tggagtaaga acccatcccg
     4501 cccgtattcg ggccctcgtc accctcgaat gccctcttgt gatcctggac aagaggcatg
     4561 gggacgaggt gtgtcccgtc gacgaacgcc tggagtgtga actcctctcc tacgagtttc
     4621 tcctcgagca cgatgtcgga tcccaggctc atgatgtact cgatcgcccc tttcctgtcg
     4681 aggtgctctc ccatgatccg gacccccttt cccccggtga gtcctgcagg cttgattgcc
     4741 aggtccccat catattcttc caggaaccca accgcgtctg ttgcagcccg gaatacccgg
     4801 taccgggggc acccggcaat cccatgctgc tccatcatta cacggcagaa cgccttgtct
     4861 gtctcgaggc gggctgccgc tctcgtgggc cccatgcagg ggattccgtc cgcttcgagc
     4921 ctgtccacga cacccgcctc gagcggggcc tcgggcccga taattgcata atctaccgat
     4981 tttttcttgg caaacgccga tacagaatcg acccgggtct ctttctccag gagaaagtcc
     5041 cggcagagcc gcgagatccc cgggttttca cgggccatga cggcatagag ttctgtctca
     5101 ttgttgcggg agagtgcacg ggaaatggca tgctccctcc ccccaccccc cacaaccagg
     5161 atcttcatag tcatgtatcg gaaccggtac tcttcacgat tgctgttctt ctctcctgcc
     5221 gatgacatcg gatgccctga caagtgcgtc cagtgcaacc ccgttctcgt gaaggagcgc
     5281 agtggcaccc gattccctgt caaccacggt cacgactcga ttcacagtcg cacccgcatc
     5341 ccgaagggcg cagattccaa agagtgcgga tccaccggaa gtcgtcacgt cctccacgag
     5401 gagtacatgt ttccccctga catctccgat gatccttccc gctttcccgt ggtgcttctc
     5461 ctggcttcgg acgatggcaa agggtttcat gctctcgagg gagacggcca ccgcaagggg
     5521 gactcccccg accgcaaccc ccgccaccac ttcgaacgca tagtcccttg cgatagctcc
     5581 tgcgatctct ttgagggccg ccggctgtgt caccactttc ttgatgtcaa gataatacga
     5641 gctccgggca cctgaagcca ggacaaaatc cccgaattcc agggctccgt actcttccat
     5701 gagatgagcg attctattta ccatggtacg tctttcactc cggtaaggta gccgatcacg
     5761 tttgcggtct tatggaggag gggagtgagc acgagtatcc agaag
//
LOCUS       DAOQVJ010000032         5768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_15344_length_5768_cov_4.150009, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000032 DAOQVJ010000000
VERSION     DAOQVJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15344_length_5768_cov_4.150009"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKL26_01265"
     CDS             complement(<1..409)
                     /locus_tag="PKL26_01265"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HNS82353.1"
                     /translation="MLPAYVPNSVAAALGGGRPIDNGRYFSDGRRIFGDGKTYRGFFA
                     GVAGGILAGGMQIWLQDIFPISSLPAQTLLSIVLLAFGALLGDLAKSFFKRRLGKASG
                     ERWPVADQYDLVVGAFVMLAIFDLPWLLQTVTLP"
     gene            545..2017
                     /locus_tag="PKL26_01270"
     CDS             545..2017
                     /locus_tag="PKL26_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS82354.1"
                     /translation="MAVILKKTRSLCPTCNAVLEAAVVEEDGKVWLERSCPEHGHFRD
                     VYWSDAELWKKFERYESLGKGIENPHRHAPPDGCPHYCGICSNHKSSTLLANIDLTNR
                     CNLNCDFCFANAQACGYIYEPTFEQVVEMMKLLRAELPVPAPAVQFSGGEPTMREDLP
                     QIIRTAKDLGFPQVQLATNGIKLAQDPTYLQELRDSGLSTLYLHFDGVSRETNSKLAI
                     DKKVIKNSEELLVGVVLVPTIIKGRNDHEVGAIIQFAAEHIRAVRGVNFQPIAFTGAA
                     SEEDVQRERITIPELLGDIEEQTYGVIKKDDFYPVPCVVPFSDFVEAYTGKPQIRFTA
                     HQHCGAATYVFVNNDGLTPVNRMVDVESLLHALTETPNKLRKGGTFNKYKALIEAIGD
                     LNESFKKSEHGNSSEFWKLLGKALLFQNFEALREFHWNALFIGTMHFMDRYNYDLSRV
                     QRCCIHYATPDGTLIPFCTYNSGPVYREIVWKKWSAPLPE"
     gene            complement(2077..3012)
                     /locus_tag="PKL26_01275"
     CDS             complement(2077..3012)
                     /locus_tag="PKL26_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330490.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNS82355.1"
                     /translation="MRYYPDPGRDIDLLKINRAVEESFAEHARGKVQMPPKVYVTLPH
                     GDFRTMPAYIPSLDIAGVKVVNVHPGNPSRGLPTVMALTVILDVETGHPRAVLNATEL
                     TDLRTGASGAVAAAHLSPRRSVGLGLVGSGRQAKSQVRAIEKELDIHEVLVWSRSEKN
                     AIQLCSEFGHLDCRSASLERVCGCDLLVTTTPSREPLVQDDWVMEGTHINAIGADAPG
                     KEELDPAILMRARVFVDDPVQAVHSGEVNVPIASGLYSEDMIAGTLGQVVIGEKGRMN
                     PDEITVFDSTGLAIQDLAIAAIALESGEGMPLRFP"
     gene            3158..3952
                     /locus_tag="PKL26_01280"
     CDS             3158..3952
                     /locus_tag="PKL26_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNS82356.1"
                     /translation="MIHIVRKPTDTPGDDSTSMVVKEITRQGGRYSFLDLDRVDPFSS
                     PLSGALIWICGLRQDEHQFEVCHALSVTNRVVNTPLAIFTCASKVLTSTLLVKNGIPT
                     PRTFFTSSGQTAADLIARFGKMVTKPVYGFDGNGISLVDSPEQLGAPPYYLQEYVPND
                     RDFRVFVIDGKAVGAISRISDSLTHNIHQGGTGKPVGIDDRMGELAGAAARAVGVDYG
                     GVDLLPSGDDYCVLEVNGTPNWHCMGVNIPGLLAGYLLSQERLLKG"
     gene            complement(3980..4264)
                     /locus_tag="PKL26_01285"
     CDS             complement(3980..4264)
                     /locus_tag="PKL26_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HNS82357.1"
                     /translation="MHKEELITLHQMLYEIKDYFESTNSDLKFNQYYSLKINPSQLHK
                     SKMEHKYAIFILGNELANAMKDVEFSASGRISARMKELAVKTLKEMEYIQ"
     gene            complement(4326..5570)
                     /gene="dnaG"
                     /locus_tag="PKL26_01290"
     CDS             complement(4326..5570)
                     /gene="dnaG"
                     /locus_tag="PKL26_01290"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HNS82358.1"
                     /translation="MYSSDTTKYMIHIHLEAEGVVEKPDVVGAVFGQTEGLLGEDLDL
                     RDLQRTGRLGRIDVQVTSKKGETKGEIYIASSLDRAETAILAASFETIDRVGPCVARV
                     RVESIEDIRVTKRRQVISRARELLMDAFEEGTIDSNVLIDEVRESMRMEKISTFGEDK
                     VPAGPNVADSDAIIVVEGRADVLNLLRFGIKNAVAVEGTRISRTIVDLCEKKTATAFF
                     DGDRGGDLILRELMQVAEIDYVAYSPRGKSVEDLGRKEIVKALRNKIPADYVREELSG
                     GEEGEASDLSTTSLPHENGSRKSRESDLSQKKRENGKRSEPPASPAPRTLTDHLEAMK
                     GSRSARFLSRDFTILRDVSLQEVERALEEMNGDATGLLLDGEVNQRLLDTVAGKGLEY
                     IAAKEYKGIIKRPLSIKILKIA"
BASE COUNT         1285 a         1701 c         1523 g         1259 t
ORIGIN      
        1 ccgggagcgt gactgtctgc aggagccatg gaaggtcgaa gatcgcgagc attacaaatg
       61 ccccgaccac caggtcatac tggtcggcaa ccggccagcg ctcaccgctg gccttcccga
      121 gcctccgctt gaagaaactc ttggcaaggt ctccgagcag agccccgaat gccaggagga
      181 caatggagag caatgtctgg gcgggaagag aagagatcgg gaatatgtcc tgcagccaaa
      241 tctgcatccc tcctgcaagg attcctcccg ccacccccgc gaagaaccca cgatacgtct
      301 ttccatcccc aaagatcctc ctcccgtccg aaaaatacct gccattgtcg atcggcctgc
      361 cgccacccag tgcagctgca acagagttgg gaacatatgc aggcagcatg caccacaaag
      421 cggctaccaa tgctattatg acggaatcag gactaatact atgctctcca gacaattgta
      481 ataaagagta gttataagca ttaagatatc ccagaagata tcaattcttg ggtggattgg
      541 tgacatggcc gttatattga agaagaccag gagtttgtgc ccgacctgca acgcggtgct
      601 ggaggcagcc gtggttgagg aggatgggaa ggtatggttg gagcgctcct gccccgagca
      661 tggtcatttc cgggatgtat actggtctga cgcagaatta tggaagaagt tcgagcggta
      721 cgaatccctg gggaagggaa tcgagaatcc ccaccgtcac gcacccccag acggctgtcc
      781 ccattactgc gggatatgca gcaaccacaa gtccagcacg ctccttgcaa atatcgacct
      841 caccaaccgc tgcaacctca actgcgactt ctgttttgcc aatgcacagg cctgcggtta
      901 catatacgaa cccacgttcg agcaggtggt cgagatgatg aagctgctcc gggcagagtt
      961 gccagtcccc gcacccgcag tccagttttc gggaggggaa cccacgatgc gggaggacct
     1021 cccccagatc atccgcaccg caaaggatct gggtttccct caggtccagc tggcaacgaa
     1081 cggcatcaag ctggcgcagg accccacata cctccaggaa ctccgcgact caggcctctc
     1141 gaccctgtat ctccactttg acggcgtgag ccgggaaacc aactcgaagc tggccattga
     1201 caagaaggtc ataaagaaca gcgaggagct cctggtaggg gtggtgctgg tgcccactat
     1261 catcaagggg agaaatgacc acgaggttgg ggcaatcatc caattcgcgg cagaacacat
     1321 ccgggcagtc aggggcgtaa acttccagcc catcgctttt acaggcgctg cgagcgagga
     1381 ggacgtgcag cgtgagcgaa tcaccatccc cgagctcctc ggagacatcg aagaacagac
     1441 ctatggggtg ataaagaaag acgactttta ccccgtgcca tgcgttgtcc cattctcgga
     1501 tttcgtggag gcctacacgg ggaagcccca gatacgtttc accgctcacc agcattgcgg
     1561 cgcggcgact tacgtctttg tcaacaacga cgggctgacg cctgtaaaca ggatggtgga
     1621 tgtggagagc cttctccatg cactcacaga aacccccaat aagctcagga aaggcggcac
     1681 attcaataag tacaaggcac tcatcgaggc tatcggagat ctgaatgaat ctttcaagaa
     1741 gagcgagcac gggaacagtt ccgaattctg gaaactcctc ggaaaggctc tcctcttcca
     1801 gaacttcgaa gccctccgcg agttccactg gaatgccctc tttatcggga ccatgcattt
     1861 catggaccgg tataactacg atctctcgcg cgtgcagcgc tgctgtatcc actatgccac
     1921 cccggacggc acgctgattc ccttctgcac ctacaatagc gggcccgtct acagggagat
     1981 cgtctggaaa aagtggagtg ctccccttcc cgaataaggg ggccatcttt tttccgggcc
     2041 cggcctcccc cttgtttttg atggcttgcg gcctgatcag gggaacctga gtggcatccc
     2101 ttcaccggat tccagtgcga tcgcagcgat ggcaagatcc tggatcgcaa gtccggtcga
     2161 gtcaaacacc gtgatctcat cagggttcat ccgtcccttc tccccaatga cgacctggcc
     2221 aagggtccct gcgatcatat cctcggaata gagcccgctc gcgatcggga cgttcacctc
     2281 gccggaatgg accgcctgca caggatcgtc aacaaaaact cttgcccgca tgagaatagc
     2341 cgggtccagt tcctccttcc cgggggcatc cgctccaatg gcattgatat gcgtcccttc
     2401 catgacccaa tcatcctgga ccaggggttc cctcgaaggg gtcgtggtga ccagaaggtc
     2461 gcacccgcaa accctttcaa ggcttgcgct cctgcaatca aggtggccga actccgagca
     2521 cagctggatg gcattcttct cgctcctgct ccagaccagg acctcgtgga tatcgagctc
     2581 tttctcgatg gcccgcacct gcgattttgc ctggcggccg gagcccacca gcccgaggcc
     2641 gacagacctg cggggggaaa gatgcgctgc cgccaccgca cccgatgccc cagtcctcaa
     2701 gtcggtcaac tccgttgcgt tcaggacggc acgcgggtgt cctgtctcca cgtcaaggat
     2761 gaccgtgagc gccatcactg tgggaagacc cctcgacggg ttgccggggt ggacattcac
     2821 gacctttacg ccggcgatgt caagagaggg aatataggca ggcattgtcc ggaaatcacc
     2881 atggggaagg gttacgtaca cttttggcgg catctgcact tttccacggg catgctctgc
     2941 aaacgactct tcaacggccc tgtttatctt caggagatcg atgtctctcc cggggtccgg
     3001 ataatagcgc atgcttgctg gtacggtgcc aggaaagaat aatgtgatgg catggatgcc
     3061 ctgccagact cgattgtacg gctcaattcc agtgagtata agtcacactt ataccctcca
     3121 gactccaacg ggatcaccgt acaaggggca tcataccatg atccatatcg tacgaaagcc
     3181 taccgatacc cccggtgacg actcgacctc catggtggta aaagagatca ccaggcaggg
     3241 aggccgttat tccttccttg accttgaccg ggtcgacccg ttcagcagtc cactctcggg
     3301 tgcccttatc tggatctgcg gactccggca ggacgagcac cagttcgagg tctgccatgc
     3361 cctctcggtc accaacaggg tggtcaacac gccgctcgca atcttcacct gcgcaagcaa
     3421 ggtcctcacc agcaccctcc tcgtcaagaa cggcatcccg acaccccgga ctttcttcac
     3481 ttcttctggg cagacggctg cagacctcat cgcccggttc gggaagatgg tcacaaagcc
     3541 cgtgtacggg ttcgatggaa atgggatctc ccttgtagat tctccggagc agctcggagc
     3601 tccaccctat tacctccagg aatacgtgcc gaatgacagg gacttccgcg tatttgtcat
     3661 cgacggcaag gcagtgggag ccatctcccg gatctctgat tccctgactc ataacattca
     3721 tcagggcggg acaggaaagc cagtcgggat tgatgaccgg atgggtgaac ttgcgggggc
     3781 agctgcgaga gccgtcgggg tggactatgg gggtgtcgac ctcctcccgt ccggtgacga
     3841 ttactgtgta ctcgaggtga acgggacccc caactggcac tgcatgggag tgaacatacc
     3901 tgggctcctg gcggggtatc tgctctcgca ggaaagactc ctgaaaggat gaatgcgaag
     3961 gacgaggaaa acagggtgct tattggatat attccatctc ttttaatgtc ttcacggcaa
     4021 gctctttcat cctggccgat atacgtcccg acgcggagaa ctccacgtcc ttcatggcgt
     4081 ttgcaagctc gttacccagg ataaagatgg cgtatttatg ctccatcttg cttttatgaa
     4141 gttgcgaggg gttgatttta agagaatagt actggttgaa cttgagatct gaattcgtgg
     4201 actcaaaata gtccttgatc tcatagagca tctggtgcag tgtgatcaat tcctctttgt
     4261 gcatcctctc acctccagaa gatgattgtt gcactgggga taaataggta acccctgaaa
     4321 aaaaattaag cgattttaag gatttttatc gaaagcggac gctttataat gcctttatac
     4381 tcctttgccg cgatatactc aagacccttc ccggcgacgg tatccaggag tctctggttg
     4441 acttccccgt caagtaaaag cccggtggca tcaccattca tctcttcgag ggctcgttcc
     4501 acctcctgga gcgagacatc acggaggatg gtaaagtccc ttgagaggaa ccgtgcactt
     4561 ctggaccctt tcatggcttc gagatgatcg gtgagggtac gcggggcggg tgatgcggga
     4621 ggttcggatc gcttaccgtt ctcccgcttt ttctgcgaga gatcgctctc cctgctcttc
     4681 ctggagccat tctcatgcgg aagggaggtg gtggagagat cggaagcctc gccctcttcg
     4741 cccccggaaa gctcctcccg gacataatct gccgggatct tgttccgaag cgccttgaca
     4801 atctcttttc ggccaaggtc ctccacgctc tttccacgcg gcgagtatgc cacgtaatcg
     4861 atctccgcaa cctgcatgag ttcgcggagg atgagatctc caccccggtc cccgtcaaaa
     4921 aatgcggtcg cggtcttctt ctcgcagagg tcgacgatcg tcctggagat acgggtgccc
     4981 tcaacggcca ccgcgttctt gatgccaaaa cggagcaggt tgaggacatc cgcccttccc
     5041 tcgaccacga tgatggcatc agaatcagcg acattggggc cggcagggac cttgtcctcc
     5101 ccgaatgtag agatcttctc cattctcatg ctctccctga cttcgtcgat cagaacattg
     5161 ctgtcgatgg tcccctcctc gaacgcatcc atcaggagct ctcttgctct cgagatgacc
     5221 tgcctgcgct tcgtgaccct gatatcctct atcgattcca cgcgcactct cgcaacgcag
     5281 ggacccacgc ggtcgatggt ctcgaacgat gcggcaagta tggccgtctc cgcccggtcg
     5341 agggatgacg caatgtagat ctcccccttt gtctctcctt tcttgcttgt cacctggacg
     5401 tctatccttc ccaggcgacc cgtcctctgt aagtcccgca ggtcaaggtc ttcgccgagc
     5461 aacccttcag tctggccgaa cacggccccc actacgtcag gcttctcgac caccccctcc
     5521 gcctcgaggt gaatatgaat catatacttg gtagtatcgc tggagtacat acaccccact
     5581 ccgtgaatcg taatctgtca cccaaccaat gccttgctga attgccgcac ctgtcatgtg
     5641 acaaatataa gttagatatt ataatatatg aatatatttt tgcgaaacct gcaccaggac
     5701 tcaagtacag gggattgcgg tgccatgaga tcacctcccg cggctgggag cactttccag
     5761 gattttct
//
LOCUS       DAOQVJ010000033         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_15664_length_5670_cov_5.113268, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000033 DAOQVJ010000000
VERSION     DAOQVJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15664_length_5670_cov_5.113268"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PKL26_01295"
     CDS             complement(<1..1243)
                     /locus_tag="PKL26_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNS82359.1"
                     /translation="MDKQDFEAILNGSKDITSYMQMNPLEGVTTCFGVRTASNPNYMV
                     KALYEMYEANLNRKLANKAMRKLFRSVGQGSVGLDKLLKKLGLNLKPEEFLMLVFKLQ
                     HQQGWGSPFELVSASDKRIVLRCKQTFESEVLKDWKMPVCGLHQGWIEGILSAITGKT
                     WYCLEKSCHANGDPYCEFVADRVEPSWKFKAEAVVRGESAITEFIEHKPLEGKISLMD
                     EPVVMMPRFIFTSMTNSLKKTMGEAPASGVNYRAYMDMGKENVEHYKKMSIYFDLSRT
                     KLDGDLKLLHDRFAAYGSPSITGGKITYGDHVVNDNFEVVDSIEKERGSKATVFQKTG
                     DKAIRISTNVIGADGKRAVGTEISSVVYNAVINKGQTYYGTADVVGKKYVTAYEPIKN
                     PAGETIGILFVEFDDPYALWLD"
     gene            complement(1347..1970)
                     /locus_tag="PKL26_01300"
     CDS             complement(1347..1970)
                     /locus_tag="PKL26_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82360.1"
                     /translation="MTSIEILDSLIAESAGDGPASVQALLQMARSKGVYGIARAVERD
                     QRHYILFFAGEPDGAVFNDHKGMLFGNKAVYILKGTEQFTFYPVDRTIIERIILGCRI
                     FDRNILNRMLPSDIPQVTPKREGGAGVFAMRVVKEGKPVSGQRVSIRKGGQIVGNDFT
                     SAEGRVSFRLLYDRYECVVHLRDLSTRVYEFEFHPGLLNQVVDLDIS"
     gene            2134..2925
                     /locus_tag="PKL26_01305"
     CDS             2134..2925
                     /locus_tag="PKL26_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNS82361.1"
                     /translation="MIIPLAIGSAAVLCTFLYASCRDLKERRVPFRTWYPMLAVTLPM
                     AAWFYASFLIMGEWTIPAYFLALTAIFAVVFYLFAWLNLFGGADAWALIFLSVSIPAF
                     PIEPLSGYPPAGFFPFAVLVNALLLNLLTPLLLGLQNLLRGQRAPFPYMLLGYPVPAV
                     ELPGAYGFIMEDIEENEDGSITRRFVRPLEAVRRMFSGEKRIYTKDLRLHPENYSKEI
                     ALFKLAGQVWISYGIPFIVPLTAGFLSALFFGDILFFLIKSVSGV"
     gene            2927..3295
                     /gene="hisI"
                     /locus_tag="PKL26_01310"
     CDS             2927..3295
                     /gene="hisI"
                     /locus_tag="PKL26_01310"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNS82362.1"
                     /translation="MLKLSYADGLIPVVVQDAGTRAVLMVAYANAEAVELTKNTGCAH
                     YFSRSRKKIWKKGEESGHLQKVLRILVDCDEDCLLYEVEQAGAACHMGYRSCFFRTIN
                     GDIVEEKVFDPEMVYAKKGH"
     assembly_gap    3661..3760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3920..>5670
                     /locus_tag="PKL26_01315"
     CDS             3920..>5670
                     /locus_tag="PKL26_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HNS82363.1"
                     /translation="MKLVPDTSVVIDGRITSMIQTGEYKGASIIIPEAVVAELEAQAN
                     QGREIGFSGLTELQALCKLAEEGTIELRFVGVRPTLEQVKLASGGEIDALIRHAAIEN
                     SAKFITSDVVQAEVAKAKGLDVVYLRPQVEGFTPLGIDQFFDEFTIAVYLKERVSPMA
                     KKGTVKEMRLMKIRDQLCTDYELRNLAQEILERAKRDPDGFIELEKRGVTVVQIGSMR
                     IAITRRPFSDGMEITAVRPIADVSLEQFNKANIIKTRIVGDKRGLLIAGSPGGGKTTL
                     AQSIATYLAEHGYVVKTMEAPRELQVPDHITQYTSLDGSMENTADVLLLVRPDYVIFD
                     ELRKNEDFRVFADMRLAGIGMIGVIHAIGAHDALQRFSDRVDFGVLPQIINTIIFVDK
                     GEITNIYDVGFTIKVPEGMSSEINLRPVTTVSDYETGDLVFEIFKYDGETIVMPVMSA
                     GTAPAPLKTPAPLKEEENTQWKILEKEIQREIGRYTDGYVDVHMLSDSKAVVYIEDKD
                     VPAAIGKGGKNIAAIVNKVGIGIDIRPRTELEKVPAAPVQEEELQLGGGVKIRMDKKQ
                     LAIICPEQSGKIVDVFSG"
BASE COUNT         1335 a         1464 c         1451 g         1320 t
ORIGIN      
        1 gatccagcca gagagcgtaa ggatcatcga actcaacaaa caggatacct atggtctctc
       61 ctgcaggatt tttgattggc tcatacgccg tgacatactt cttcccaacc acatctgcag
      121 tgccgtaata ggtctgccct ttgttgatca cggcattgta gacgacactt gaaatctcgg
      181 ttccaacagc ccgcttgcca tcagcgccaa taacgttggt agagatacgg attgccttat
      241 caccggtttt ctggaagacc gtggccttgc tgccccgttc cttttcaatg gaatcaacaa
      301 cctcgaagtt atcgttgacg acatggtcgc cgtatgtaat cttgcctccg gttattgaag
      361 gagacccata tgctgcgaaa cggtcatgca acagtttgag atccccgtca agtttcgtcc
      421 tggagaggtc aaaatagata ctcatcttct tgtagtgttc cacgttctcc tttcccatgt
      481 ccatgtaggc acggtagttg acaccgcttg ccggcgcctc tcccatggtc ttcttcagcg
      541 aattagtcat ggacgtgaag atgaaccggg gcatcatgac aaccggttca tccataaggg
      601 agatcttccc ttcaaggggt ttgtgctcga tgaactcagt gattgctgat tcccctctga
      661 ccactgcctc tgccttgaac ttccagcttg gctcgacccg atcggcaaca aactcacaat
      721 acgggtctcc gttcgcgtgg caactcttct cgaggcagta ccaggtcttc ccggtaattg
      781 ctgaaagaat accttcaatc catccctgat gaaggccaca gaccggcatc ttccagtcct
      841 tgagcacctc ggactcaaac gtctgcttgc agcgaagcac tatgcgcttg tcacttgcac
      901 tgaccagttc aaagggggaa ccccagccct gctggtgctg aagtttgaag acgagcatca
      961 ggaactcttc cggcttcagg ttcagaccga gtttctttaa gagtttatct agcccgactg
     1021 atccttgtcc cacagaacga aacagtttcc gcatcgcctt gttggcaagc ttcctgttaa
     1081 ggttggcctc gtacatctca tagagagcct tgaccatgta attcgggttg cttgcggtcc
     1141 ttaccccaaa acatgtggtg accccctcga gcgggttcat ctgcatgtag gaggtaatat
     1201 ccttgctccc attgaggatg gcctcaaaat cctgcttatc cattggatcc ttctcctcaa
     1261 tccagatatc tgatgtaagc tcacttaaaa atgtagcatg tccttttcat gccgcgataa
     1321 ccacagcctt gcagaatctt cctcaattac gagatatcga ggtccaccac ctggttcaaa
     1381 agaccaggat ggaattcaaa ttcatagacc cgcgtggaga ggtcacgaag gtggaccacg
     1441 cactcgtaac gatcataaag aaggcgaaaa ctgaccctgc cttccgcgct ggtaaaatca
     1501 tttccgacaa tctggcctcc cttccggatg gagaccctct gaccgctgac aggctttccc
     1561 tcttttacaa ccctcattgc aaacacccct gcgccaccct ccctttttgg tgtgacctgc
     1621 gggatgtcgc ttgggagcat gcgattgagg atattcctgt cgaatatccg gcacccaagg
     1681 atgatccttt cgatgatggt acggtcaacg gggtaaaaag tgaactgttc cgtccccttc
     1741 aggatgtata cagccttatt cccgaagagc atccccttgt gatcattgaa aacggcacca
     1801 tcgggttccc ccgcaaagaa aaggatgtag tgcctctggt ctctctcaac cgctcgtgca
     1861 atgccgtaca ccccttttga ccgggccatc tgaaggagag cctggactga tgccggtcca
     1921 tctccggccg attccgctat cagcgagtcc aggatctcaa tactggtcaa tgaagatcat
     1981 ctcctgcccg tctcattgtt catctcatct ctcgcgatat catgaccgaa ggggaggatt
     2041 gaggggttcc atgcgattcc atgggcctcg tgttgcaggt gctgctccgg ggccatgcat
     2101 aagaacctgc acgactcaga tgtacctgta tccatgataa tccctctggc aataggttca
     2161 gcggcggtgt tatgtacttt tctctatgca tcttgcaggg atttgaagga gcggagagtt
     2221 cctttccgca cctggtatcc catgcttgct gtcaccctcc ccatggctgc gtggttttat
     2281 gcctcgtttc tgataatggg agagtggaca atccccgcat atttccttgc gttgactgcc
     2341 atattcgcgg tggtatttta tctctttgcc tggctgaacc tctttggcgg ggctgacgcc
     2401 tgggccctca tcttcctgtc ggtgagcatc cctgcatttc ccatcgagcc gctgtccggg
     2461 tatccccctg cagggttctt tccattcgcg gtgctcgtga acgccctcct cctgaacctt
     2521 ttgactcccc tgttactggg cctgcagaac ctgctgcgtg gacaaagagc gccttttccg
     2581 tacatgttgc tgggatatcc tgtccctgcg gtcgaattgc cgggggctta tggttttatc
     2641 atggaggata ttgaagagaa cgaggacggg tccatcacga ggcggtttgt gcgaccgctg
     2701 gaggctgtaa ggaggatgtt ttcaggtgaa aaaaggatct ataccaagga cctccggctt
     2761 catcccgaga actattcgaa ggagattgcc ctcttcaaac tggccggcca ggtatggatc
     2821 tcatacggga tcccatttat tgtccccctg actgcagggt tcttatccgc gctcttcttt
     2881 ggagatatcc tctttttcct gattaaaagt gtttctggtg tataatatgc tcaaactttc
     2941 atatgcagac ggactaatcc cggtggtggt gcaggatgcc ggcaccaggg cagtattgat
     3001 ggttgcttat gcgaatgcag aggcggtcga actaacgaag aatactgggt gtgctcatta
     3061 tttctcacgc agcaggaaga agatctggaa gaaaggagag gagagcggac acctgcaaaa
     3121 ggtgctccgc atcctcgtgg actgcgacga ggattgcctg ctctatgagg tggagcaggc
     3181 cggtgcggcg tgccacatgg gataccgatc atgctttttc aggaccatca acggagatat
     3241 tgtcgaagag aaggtatttg atccggaaat ggtatatgcc aaaaaagggc attgaagggc
     3301 tttttcacac tacacatttt ttcgtaggat tctcatgctg ggtccccgca gtacttcatg
     3361 agaacctgaa ggagatcaca ctaatagggt ttgaaaaacc tggtatccgc atccgttaca
     3421 atgtgtatgc agcaaaatcc gaaatatcac acatgccagg atattgatga gggggcgtgg
     3481 aaattgggct cgaggttatc ggagccttct tttctaaaaa ccacataagt attattgaga
     3541 cacatgcata caaaatgaaa atcgcggatg cgactttcaa agtaaaaaat catgctcccc
     3601 tgcacgacag aactttcatg tatttccggg agtaatgata atcgtggacc ttataatgac
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacagaactt tcatgtattt
     3781 ccgggagtaa tgataatcgt ggaccttata atgacggctg tggggcacca tacactgctg
     3841 atcagagata cttccagtga cgaaaatatg cccaaaccat gcccgaatcc acaatataaa
     3901 acaaaaaagg gtgaaaattt tgaaattagt accggatacc agtgtagtca tcgacggtcg
     3961 aatcacctcc atgatacaaa ccggagaata taagggtgca tcaataatca taccggaagc
     4021 cgttgtcgcc gaactcgagg cccaggcaaa ccagggacgg gagattgggt tttcagggct
     4081 caccgagctt caggcgttgt gcaaacttgc ggaagaaggc accattgaac tcagatttgt
     4141 aggggtgcgt cccactctcg agcaggtgaa actggcgagc gggggggaga tagatgccct
     4201 gatccgtcat gctgccatcg agaacagtgc caagttcatc acaagcgatg tcgtgcaggc
     4261 cgaggtggcc aaggccaaag gtcttgacgt ggtgtacctc cggccacagg tcgaaggctt
     4321 cacgccgctc ggcatcgacc agttctttga cgagtttacc atcgcggtgt acctcaagga
     4381 gagagtctcc ccgatggcaa agaagggcac ggtaaaagag atgcggctga tgaagatacg
     4441 ggaccagctg tgcacggact atgaactcag gaacctcgcc caggagatcc tggagagggc
     4501 aaagcgtgat cccgacgggt tcatcgagct tgagaagcgg ggtgtgactg ttgtccagat
     4561 tggttccatg cggatcgcca tcacaaggag accgttctcc gacgggatgg agattaccgc
     4621 ggtacgccca atcgccgatg tgagcctcga gcagttcaac aaggccaaca tcatcaaaac
     4681 acggatcgtg ggcgataagc gcggccttct catcgctggt tcgccagggg gcgggaaaac
     4741 gacgctcgca cagagtatcg caacctacct tgcagagcat ggatacgtgg tcaagacaat
     4801 ggaagccccc cgcgagctgc aggtccctga ccacatcacc cagtacacgt cccttgacgg
     4861 gagcatggag aacaccgctg acgtactcct gctggtgcgc cctgactacg tgatatttga
     4921 cgagctccgc aagaacgagg acttccgggt ctttgcagac atgcgccttg cgggaatcgg
     4981 gatgatcggc gtcatccacg cgattggcgc ccacgacgcc ctccagcgtt tctccgacag
     5041 ggtcgatttc ggtgtgcttc cccagattat caatacgatc atattcgtgg acaaagggga
     5101 gatcaccaat atctacgatg tcgggttcac catcaaggtg cccgagggaa tgtcttcgga
     5161 gatcaacctg cgccccgtca ccacggtctc ggattacgag acaggggacc tggtctttga
     5221 gatcttcaag tatgacggcg agacgattgt catgccggtg atgagcgcag ggacagcacc
     5281 cgccccctta aaaaccccgg ctccactaaa agaggaagag aatacccagt ggaaaatcct
     5341 cgaaaaagag atccagaggg agatcggacg ctacactgat ggctatgtcg atgtgcacat
     5401 gctctccgat tcaaaagccg tggtgtacat cgaggacaag gacgtcccgg ccgccatcgg
     5461 gaagggagga aagaacatcg cggccatcgt gaacaaggtg ggtattggaa tcgacatcag
     5521 gccccggact gaacttgaga aggtccctgc cgcccccgtg caggaagagg aactccagct
     5581 gggaggcggg gtgaagatcc gcatggacaa gaagcaactg gccatcatct gcccggagca
     5641 gagcgggaag atcgtggacg tattctcggg
//
LOCUS       DAOQVJ010000034         5584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_15983_length_5584_cov_4.925303, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000034 DAOQVJ010000000
VERSION     DAOQVJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15983_length_5584_cov_4.925303"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKL26_01320"
     CDS             complement(<1..251)
                     /locus_tag="PKL26_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS82364.1"
                     /translation="MSKSRIFIVEDDDVIARLMEWRVKKLGYDVCGRAAEGKEAISLI
                     QSMSPDLVLLDIGLPGNCDGISVAHEIRRTHDLPIVFVTG"
     gene            558..1253
                     /locus_tag="PKL26_01325"
     CDS             558..1253
                     /locus_tag="PKL26_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNS82365.1"
                     /translation="MKYEIKYKPSYSLLVVTLAPGESIAAESGSMTYMQPTIEVKTQK
                     REKSFWGSLGLALVGGQSFFINEFTATTGTGEAGFSAAPLGDIDTIAVSQGKGFILQK
                     AAYIASQGTVNLDVKWEGFTKGLFGQGLFMIRATGEGTIFINTFGAIDRHTLKDGEKL
                     VVDNFHLVAFSDTCQYKVTKFGGLKETILGGEGLVTEIQGPGEVFIQTKNIRELADWI
                     WTIIEPRVQAKAR"
     gene            1398..1916
                     /locus_tag="PKL26_01330"
     CDS             1398..1916
                     /locus_tag="PKL26_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNS82366.1"
                     /translation="MESQHEGRIPDGTGWIRSPLKLLAAVIICNMAGIIGSFVTVTGE
                     GSWYAAIQKPPFNPPGFVFAPVWTTLYILMGISLYIVSMEGMERREVRVAIALFAVQL
                     ILNTIWSFLFFGLQSPFLGLVEIIILWIFIVATTVAFYRINKKAAYLMVPYLAWVTFA
                     AFLTYNIWLLNG"
     gene            complement(2074..2544)
                     /locus_tag="PKL26_01335"
     CDS             complement(2074..2544)
                     /locus_tag="PKL26_01335"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNS82367.1"
                     /translation="MQIHRIILLSALAVLACASAPVMAATYQFSLENNGDSVNVIPGD
                     TITLSLKENPSTGFRWIMETSGGLQTLRDTYSPSSTGLIGASGMRTWKFLVTGTGTHT
                     ISAIYKQSWMPTTGDEQKFTLDVISGYAAIQKPVSTSKFRSYQKPVLYKTVHYP"
     gene            complement(2973..3713)
                     /locus_tag="PKL26_01340"
     CDS             complement(2973..3713)
                     /locus_tag="PKL26_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82368.1"
                     /translation="MKKDICDFTATLCHFMVPLPVIEVGEKEFQLSSFLRAYWFLFIF
                     VSIFTALTAFLTQTFSGHGSREPIFELLGIPFDLQEISIASCLLITYLGVFALLWAAF
                     AEPRDRPIFYYFIGVEAFKRLLLVIPLVVLMLTLTTWINLVHTQVMAVIFSLIGFCLG
                     IVVFSSILVLVSRNTRIRRFVLATMTYALVVFVVSLASLLAINLRSVPLVYTPAIFFI
                     YAMGISSVFYIIATLIFEIILPLFQGWK"
     assembly_gap    3976..4075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4076..4758
                     /locus_tag="PKL26_01345"
     CDS             <4076..4758
                     /locus_tag="PKL26_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS82369.1"
                     /translation="TSGDQALEVLAHHKFDAIISDYAMPGMNGIEFMKQVKALGDDTP
                     FIIFTGKGREDVVIDALNFGADLYLEKSPDPRTQFSEMMQKVRNIVTIRADVQVAREK
                     MRIFSSVMERFPGMIFRMQLNGRWAFLFASAGSERLTGYSGDTLVKGDPAFEMIVIPD
                     DRERVDQERRSALYSGGEYLVKYRIRTSLGEEKRVVEGGFVVPGSDEEILEGYILDLD
                     IVGNGSVR"
     gene            complement(4800..>5584)
                     /locus_tag="PKL26_01350"
     CDS             complement(4800..>5584)
                     /locus_tag="PKL26_01350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNS82370.1"
                     /translation="VIHKKDGSRVVAEIRNHPVVIGGKTVVLGSAHDVTSRHLAEESL
                     RRMNAKLTLLNIITRHDILNTVTALLLYIELLKEDPKDDAQGETLSKVEQLTQTIRRQ
                     IEFTKVYQDIGIEVPQWQSLGEIIGRASASATMKDVAVKSDIGDIEVYADPLLENVIQ
                     NLIDNSLRHGQGADSIWLTVLHDGQGMKIVYEDNGQGIPSREKEEIFKRGYGKNTGFG
                     LFLAREILAITGLTIKETGTFGKGARFEIRIPQGCFRQAQKQ"
BASE COUNT         1318 a         1383 c         1497 g         1286 t
ORIGIN      
        1 ccggtcacaa atacaatcgg taaatcatgg gttctgcgga tttcatgggc aaccgagatg
       61 ccgtcacaat tcccgggaag cccgatatca aggagtacga ggtccggaga catgctctgg
      121 atgagagata ttgcttcctt tccttctgct gcccgcccgc atacatcgta cccgagtttc
      181 ttcacccgcc actccatcag tctggcgatg acgtcatcat cttcaacaat gaatatgcga
      241 ctttttgaca ttgggccgat ttatctctcc cttttaataa aaactgcttc aattacgctt
      301 ataaaaattt gccatgcact cgttcatcgg agcaatgaat attcatcggt gcgatgaacg
      361 aaccattaaa attatcgccg attgcgatag cccggcaatc tccatctctc aggaatttcg
      421 cagcatatca gagtttactg tctcaataca atagaggtgc agcggccaga aatactgaat
      481 gcattacagt attcacctgt taccggaata gtccaggtga ggaaagccct ttctctgaga
      541 aacagagatt gtatagcatg aagtacgaga tcaagtacaa accctcttat tcgcttcttg
      601 tggtcaccct ggccccaggc gagagcatcg cagcagagtc ggggtcgatg acctacatgc
      661 agcccacgat cgaggtgaag acccagaaaa gggagaagag tttctggggc tcactgggcc
      721 ttgcactcgt gggcgggcag agcttcttca tcaacgagtt caccgccacg acaggaaccg
      781 gcgaggcagg gttcagcgcc gcccctctcg gtgacatcga caccattgcg gtcagccagg
      841 ggaaagggtt catcctgcag aaagcggcat acatcgcatc ccaggggact gtgaaccttg
      901 acgtcaagtg ggaagggttc acgaaagggc tcttcgggca gggcctcttc atgatccggg
      961 cgacgggcga ggggaccatc ttcatcaaca cgttcggggc aattgaccgt catacattaa
     1021 aggacgggga aaaacttgtc gtggacaatt tccacctggt ggcattcagc gacacctgcc
     1081 agtataaagt gacgaagttc ggcgggctga aggagacgat actgggcgga gaagggcttg
     1141 tcaccgaaat ccaggggccc ggcgaggtct tcatccagac caagaacatc agggaacttg
     1201 ccgactggat ttggacaatc atcgagccga gagtgcaggc caaggcaagg tgaaacactc
     1261 cccactttgg ccgggtcgga tcccgatcaa ccgttcccgg ggaaagtaaa tctctcctga
     1321 agaaggaacc cgtttcagtg gtgcccgaat ccttctgcga gggggacaac taaataccaa
     1381 aggcctagat aactggcatg gaatctcaac acgaggggag aataccggat ggaaccggct
     1441 ggattcgttc gccgctgaaa cttctcgccg cggtcatcat atgcaatatg gcgggaatca
     1501 tcgggtcttt cgtgacggtc acgggagaag ggtcctggta tgctgccatc cagaaacccc
     1561 cgttcaatcc gcctggtttt gtcttcgccc ctgtgtggac caccctctac atccttatgg
     1621 gcatatcgct ttacattgtc tcgatggaag ggatggagag gagggaggtc cgggtcgcaa
     1681 tcgccctttt cgcggtccag cttatactca acacgatctg gtccttcctc ttctttggcc
     1741 tccagtctcc attccttggc cttgtcgaga ttatcatttt gtggatcttc atcgtcgcaa
     1801 ccaccgttgc cttttaccgc atcaataaaa aagcggcata cctgatggtg ccgtatctcg
     1861 cctgggtcac atttgcggca ttcctcacct acaatatctg gctgttgaat ggatgaaggg
     1921 atccaaaatt cttttcacat ggtgcaagaa acgggctggc caggacccgg ctcctgcgtt
     1981 ctgaattgat atatccggaa tagcaatacc agttgtcccg cttttattga cagggtatgc
     2041 tgatccaaat aactggtgaa aaagaggata aaatcaagga taatgcacgg ttttgtacag
     2101 gaccggtttt tggtaagacc tgaacttgct tgtggatact ggtttttgga tcgccgcata
     2161 tccgctgatc acgtccaggg tgaacttctg ttcatcaccc gttgtcggca tccatgactg
     2221 cttgtagatg gccgatatgg tatgagtgcc tgtcccggtc acgagaaact tccatgtccg
     2281 catccctgat gctccgatta atcccgtgct ggacggggag tatgtgtccc gcagggtctg
     2341 taacccgcca ctcgtctcca ttatccatcg aaaaccggta ctcgggttct cttttaagga
     2401 gagggtgatg gtatcaccag ggatgacatt cacggaatct ccattgtttt ccagggagaa
     2461 ttggtaggtg gctgccatga ccggtgccga cgcacatgca aggaccgcaa gcgctgacag
     2521 gagaattatt ctgtgtattt gcattggtga acacacctct cgctgtgaga aatatcgtcg
     2581 cgttcgtata taaaaggctc cacgctatca ggtgcgggta agagtgtttg accggattca
     2641 ttctgtggag catgtattct ccctgcattt ccggtatgca atccatcgcg tcaattatgg
     2701 aattacacca aaagcttcca tttttaaacc gaaaggggca ctttggccac caatggtgca
     2761 tttggcgagg tgaaactgat ttcgggacca taaaacggat tgtagagaag gggggaaggt
     2821 ggtttggtga tcggacgggg atgcagatcc caacctctgg tccgcctccc ccatatacga
     2881 gtgcaaggag tatgtaaatg gggccacaca tgcagagggt tcctgatgga agcgcatggg
     2941 ctgggagggc aggtgaaggg tgtacgtctt atttatttcc atccctggaa gagtgggagg
     3001 attatctcga agatgagcgt ggcgatgata taaaagaccg acgagattcc catcgcgtag
     3061 ataaaaaaga tcgccggggt atacacgagc gggacactgc gcaggttgat cgcaaggagg
     3121 gatgcaagag acacaacgaa tacgaccaga gcgtaggtca tcgtcgcgag gacaaacctc
     3181 cggatccgtg tattcctgct gacaaggacc agtatggagg agaacaccac aatcccgagg
     3241 cagaacccga tcagcgagaa gatgactgcc atgacctgcg tgtggacgag attgatccac
     3301 gtggtcagcg tcagcatcag caccaccagt gggatcacaa gaagaaggcg cttgaacgcc
     3361 tccaccccaa tgaaatagta aaagataggc ctgtccctgg gctcggcgaa tgccgcccat
     3421 agcagtgcga acacaccgag gtaggtgatc agcaggcagg atgcgatgct gatctcctga
     3481 aggtcaaagg gaatgccaag cagctcgaat atcggttccc tgcttccatg acctgagaac
     3541 gtctgggtga gaaaggcggt gagtgcggta aaaatcgaga cgaagatgaa gagaaaccag
     3601 tacgccctta aaaacgagct cagctggaat tctttctctc ccacctcgat aaccggtaaa
     3661 ggcaccatga aatggcagag tgtcgctgta aaatcacata tatctttctt catgctcgcc
     3721 ggattgatat ttgcgcggaa gatgtatggc catttccgcc tgatttaaat agtacccggg
     3781 tcatcttcct gctgatgtat cctacggcac tggacggggg cgtggcatga tatcaatact
     3841 cctggtagat gacgaagtgc agttcctgga ggtgactcgc atgttccttg agaagggccg
     3901 gggcatccgg gtgacgaccg caacgtcagg agaccaggca ctcgaggtcc tcgcgcacca
     3961 taaatttgat gccatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaacg
     4081 tcaggagacc aggcactcga ggtcctcgcg caccataaat ttgatgccat aatctcggat
     4141 tatgcgatgc cgggaatgaa tggaatcgag ttcatgaagc aggtgaaggc cctgggtgac
     4201 gacacaccct tcatcatctt caccggaaag ggcagggagg acgtggtcat cgacgcgttg
     4261 aatttcggtg cggacctcta ccttgagaag agcccggacc cccggacaca gttctcggag
     4321 atgatgcaga aggtccggaa tatcgtcaca attcgggcgg acgtccaggt ggcacgtgag
     4381 aagatgcgca tattctcttc ggtgatggaa cggtttccag ggatgatctt tcgcatgcaa
     4441 ctcaacggga ggtgggcatt cctctttgcg agcgccggtt cggagcgcct gaccgggtac
     4501 tccggtgata cccttgtgaa gggggacccg gcattcgaaa tgatcgtaat acctgacgac
     4561 agggaacggg tcgatcaaga acggagatct gctctctatt cggggggtga atacctggtg
     4621 aagtaccgga tacgcaccag tttgggagag gagaagaggg ttgtcgaagg gggttttgtt
     4681 gtcccgggtt cggatgaaga gatactggaa ggatatatcc tcgaccttga tatcgtagga
     4741 aacgggtcag tccggtaggg ctcacaacca cccgttacag attggaccca tctcccttgc
     4801 tattgctttt gtgcctgccg gaaacaccct tgcggaatgc ggatctcgaa cctggcccct
     4861 ttcccaaatg tccccgtttc ttttattgta agaccggtga tggccaggat ctcgcgggcg
     4921 aggaaaagtc cgaaacctgt atttttaccg tatccacgtt tgaatatctc ttctttctcg
     4981 cgtgaaggaa ttccctggcc attgtcctcg tatacgatct tcattccctg tccatcatgg
     5041 agtaccgtca gccagatgga gtctgcaccc tgcccgtgcc gaagagagtt gtcaatgagg
     5101 ttctggatga cgttttcaag gagtggatct gcataaacct cgatatcccc gatatcgctc
     5161 ttcactgcaa catccttcat cgtcgcagag gctgatgccc ggccgatgat ctcgccgagt
     5221 gactgccatt gggggacctc gatcccgata tcctggtata cctttgtaaa ctcgatctgc
     5281 ctcctgatgg tctgggtcag ctgctccacc tttgacaggg tctccccctg cgcatcgtct
     5341 tttggatctt ccttcaagag ctcgatatac aggaggaggg cagtgaccgt attgaggatg
     5401 tcgtgcctgg taatgatatt aaggagggtg agttttgcat tcatcctgcg aagcgactcc
     5461 tcggccaggt gcctgctggt cacatcgtgt gccgaaccaa ggacaacggt ctttccgccg
     5521 atcaccaccg gatggttcct gatctcggca accacgcggc tcccgtcttt tttgtgtatc
     5581 acga
//
LOCUS       DAOQVJ010000035         5582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_15992_length_5582_cov_4.470237, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000035 DAOQVJ010000000
VERSION     DAOQVJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15992_length_5582_cov_4.470237"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKL26_01355"
     CDS             complement(<1..684)
                     /locus_tag="PKL26_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS82371.1"
                     /translation="MRARLPLFLGVFLVMALSNAIVPVLPSFAAGTTAQGAIFSAYFL
                     GAFLLVLPAGYLADRVGEVPLIRAGLLLTCVSGVMLSVAASPALVLAARLLEGFGAGL
                     FVPASLALLNARADHERMSGYFMALLNLGLLVGLLGTGWLVLQWGGVYAGIYLFTVAA
                     FIPLALSLGMRRGAAGQAGIPAETAAATLDRLKWVFSSYFWLWVSAVLIIGITGALTA
                     LHPEYTDLPP"
     gene            complement(681..2570)
                     /locus_tag="PKL26_01360"
     CDS             complement(681..2570)
                     /locus_tag="PKL26_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HNS82372.1"
                     /translation="MLIEERLKELKNKINEKVPRGITVSEVEFEGPELVIYTDDPKKF
                     ADQADLIKILARDLRKRIVVRPNILEDPEKAVQDIRAVVAENAGITDIYFDADTGEVL
                     IEAEKPGVVIGKNGATLREITKHIGWTPKVVRTPPIESVTVKQIRQYLRSVNEERKEF
                     LRNIGRRIHRDVIARDQWVRVTMLGCCREVGRAAFLISTPESRVLVDCGEKPGNSAST
                     PYLYVPEIHPLTQLDAVVLTHAHLDHCALVPLLYKYGYDGPVYSTPPTRDLSAMLQLD
                     YLDVVSKEDRKIPYSSNEVKNYIKHSITLNYGSVTDIAPDIKLTFHNAGHILGSAIAH
                     FHVGDGLYNIAFTGDFNFSKSRLFNPATNTFPRLEALVMESTYGGSGDIQPSRADAEE
                     KLYETVKNVIQRGGKVIIPAFAVGRSQEVMLALEEAMRKEKIPRVKIYLDGMIREATA
                     IHTTYPEYLNSDLRTQIFKEGLNPFLAEYFAPVDSPDLREKVINGDPCVIITTSGMLN
                     GGPVMEYLSNLAFDERNALVFVGYQADGTLGRRIQKGWREVPIGRKDTIVINLEIVTI
                     DGFSGHSDRKQLVNYIGHIQPRPEKIFTVHGDENNTIDLASSLYKRFHIETHSPMNLE
                     TYRLV"
     gene            complement(2595..3236)
                     /gene="psmB"
                     /locus_tag="PKL26_01365"
     CDS             complement(2595..3236)
                     /gene="psmB"
                     /locus_tag="PKL26_01365"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HNS82373.1"
                     /translation="MNERYQEGMKGTTTVGLVFQDGVVLASEMRATMGYLIASKKAKK
                     IYQIADRIGMTTAGGVGDAQQMARLMTVECSLYQIRRGRPMTVGAVATLLSNILNNNR
                     YYPFYVQLLVGGIDENGPSLYAVDALGGASKEEEMVATGSGSPMAFGVLEDRYQKDMT
                     EEEAVSLAVRAVRSAMKRDAGSGEGIHVVVITKEAYREIGDQEVAKHFPAVPA"
     gene            complement(3329..4174)
                     /locus_tag="PKL26_01370"
     CDS             complement(3329..4174)
                     /locus_tag="PKL26_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82374.1"
                     /translation="MIVKDYMTSDVVTVEIPGNRDDVLKILKRTGISGVPVLKDADLV
                     GIITRKDLLRKAEETQLGLLMTPNPVTIGPDSTVREAASMMVRHSVRRLPVIDDGSLV
                     GLLSVADLIAALAQMKIKDEIKDLYTSQTFAIWEETPLPLVGRIMEISGFEAIPILDA
                     ESTLQGIISERDLIRNSSIEDMVEVSDFSNGTDDDEWTWESIRDMHTISYGISKIQLP
                     DRPVKTAMVRNVISVPMNAEVSECALKMKRARVDQLPVVNGDKKLVAMLFDRDLIRAL
                     CQKDL"
     gene            complement(4171..4608)
                     /locus_tag="PKL26_01375"
     CDS             complement(4171..4608)
                     /locus_tag="PKL26_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS82375.1"
                     /translation="MFSEILVAVDGSKIGKRALEVAIEEARLWKARVHVVYVVETGLF
                     SSLPMDSTWEIMYSMLEKEGQETISDAKEQAAAKGVRLETYIRQGHAGNEIIKASREL
                     GADLIVVGSHGKSEVDRLLLGSVSSFVVGNSSVAVLVVRPSQK"
     gene            complement(4646..>5582)
                     /locus_tag="PKL26_01380"
     CDS             complement(4646..>5582)
                     /locus_tag="PKL26_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS82376.1"
                     /translation="SSTPANAPWICPAFFNAHTHLGDSLAMDLPFEGTLDEVVRPPNG
                     LKHRILRSAARKDLLAGMHLTLLTMAESGTAGCADFREGGEDGVSALREAARGVPCRT
                     IIFGREGGEAIADGLGISSARDARAGLEEAVSAAKRSGKLVAFHAGERDAGDVDEALE
                     YHPDLLIHCTHATRSQLRRIADLGIPVAVCPRANWRFGVTSSCDHPPIETMQALGCHI
                     LLGTDNAMCVQPDMWREAHFLSFVYRVTPRDALKSATLGSSLFCEPFFLKEGKKANFL
                     VIDPGKSNLAISRDLVGTLVNRAGPENIVRKVINL"
BASE COUNT         1060 a         1664 c         1619 g         1239 t
ORIGIN      
        1 cggcggcagg tccgtatact cggggtggag cgcagtgagg gccccggtga taccgatgat
       61 caggaccgcg gagacccaga gccagaagta ggaggagaac acccatttca ggcggtcgag
      121 ggtggcagcc gcggtctcgg ccggaatacc tgcctgcccg gccgcacccc tgcgcatccc
      181 aagcgagagt gcgagtggga tgaacgcagc gacagtgaag agatatatgc ctgcatacac
      241 tcccccccac tgcagcacca gccagcccgt gcccagcagc cccaccagca ggccgaggtt
      301 gaggagcgcc atgaagtacc cgctcatacg ctcatggtcc gcacgggcgt tgaggagtgc
      361 gagagacgca gggacaaaga ggcccgcacc aaacccttca agcagccgtg cggccaggac
      421 gagcgccggc gaagctgcaa ccgagagcat gacgccgctt acgcaggtga gaaggagccc
      481 ggccctgata agcgggacct ctcccacccg gtcggcgagg tacccggcag gcaggacgag
      541 caggaatgcc ccaaggaaat acgcggagaa tatggccccc tgtgccgtcg tcccggccgc
      601 aaaggagggg agcaccggga cgatggcatt cgagagtgcc atgaccagga agacccccag
      661 gaagaggggg agccttgctc tcataccagc ctgtacgtct ccaggttcat cggggagtgg
      721 gtctctatat ggaagcgctt gtagagcgaa ctggcaaggt cgatcgtatt gttctcgtca
      781 ccgtgcaccg tgaagatctt ctcgggacgc ggctggatgt ggccgatata gttcaccagc
      841 tgcttcctgt ccgagtgccc ggagaacccg tcgattgtca cgatctcgag gttgatcacg
      901 atggtgtcct tcctcccgat gggcacctct ctccatcctt tctgaatgcg ccgcccgagg
      961 gtgccgtcag cctggtaccc tacgaacacc agggcgttgc gttcatcaaa ggcaaggttg
     1021 ctcaggtact ccatcacagg accgccgttc agcatcccgc tcgtagtgat gatgacgcag
     1081 gggtctccgt tgatgacctt ctcccggaga tcgggggagt caaccggggc gaagtactct
     1141 gcgaggaagg ggttgagccc ctccttgaag atctgtgtcc tgaggtcgct gttcaggtac
     1201 tcggggtaag tcgtgtggat tgcggttgcc tccctgatca tcccgtccag gtagatcttt
     1261 acccgcggga tcttctcctt ccgcatcgcc tcctcgagag ccagcatcac ctcctgtgac
     1321 ctgccgaccg caaacgcagg gataatgact tttccgcccc tctggatgac attcttcact
     1381 gtttcgtaca gcttctcctc ggcatcggcc cgggacggct gaatatcgcc gctcccgccg
     1441 taggtgctct ccatgacgag cgcttcaagg cgcggaaaag tgttcgtggc agggttgaag
     1501 aggcggctct tgctgaagtt gaagtcgccg gtgaacgcga tgttatagag gccgtcgccg
     1561 acatggaagt gggcgatggc cgagccgagg atatggcctg cgttgtggaa tgtcagcttg
     1621 atgtcggggg cgatatcggt gacgctccca tagttgaggg tgatggagtg cttgatgtaa
     1681 ttcttgacct cgtttgagct gtaggggatc ttccggtcct ccttgctgac gacatcgagg
     1741 taatcgagct ggagcatcgc ggagaggtcc cgcgtagggg gggtgctata gaccgggcca
     1801 tcgtatccat atttatataa gaggggcacc agggcacagt ggtcgaggtg ggcatgggtg
     1861 aggaccactg cgtccagctg ggttaaagga tggatctccg gcacgtagag gtatggcgtg
     1921 ctcgcactgt tccccggttt ctctccgcaa tcaacgagga ccctcgattc cggggttgag
     1981 atgaggaacg cagcccttcc cacctcgcgg cagcagccga gcatggtgac cctgacccac
     2041 tggtcccgtg cgatcacgtc gcggtggatg cgcctcccga tgtttcggag gaactctttc
     2101 ctctcctcgt tgacagaacg caagtactgg cggatctgct tgactgtcac gctctcaatg
     2161 ggaggagtcc tcacgacctt gggggtccag ccgatgtgtt tcgtgatctc ccggagggtc
     2221 gccccgttct tcccaatcac gacaccgggc ttctcggcct cgatcaggac ttctccggta
     2281 tccgcatcaa aatagatatc tgtgataccc gcgttctccg cgaccacggc acggatgtcc
     2341 tggaccgcct tttcggggtc ctcgaggatg ttcgggcgga cgacgatccg tttccgaagg
     2401 tctcgggcca ggatcttgat aaggtctgcc tggtctgcga acttttttgg gtcatcggtg
     2461 tagatgacca gttccggccc ttcgaactcc acctccgaga cggtgattcc ccttgggacc
     2521 ttctcattga tcttgttctt gagctctttg agtctctctt ctataagcat caaaacctac
     2581 ccgaaaaaaa ggagttatgc ggggacggcc ggaaagtgtt tcgcgacttc ctggtctcca
     2641 atctccctgt atgcctcttt tgtgatgacc accacgtgga ttccttcccc ggagcctgca
     2701 tctctcttca tggccgagcg tactgcccgc acggccagcg ataccgcctc ctcctctgtc
     2761 atgtccttct ggtacctgtc ttcgagtacg ccgaatgcca tcggcgaacc ggacccggtg
     2821 gcaaccatct cttcttcctt tgatgctcct ccgagtgcat ccaccgcgta gaggctcggc
     2881 ccgttttcgt ctatcccgcc gacgagcagc tggacataga atggatagta tcggttgttg
     2941 ttgaggatgt tggacaggag tgtggcgact gcgccgactg tcatgggtct gcccctcctt
     3001 atctggtaga ggctgcattc gacggtcata agccgtgcca tctgctgtgc gtcccccacg
     3061 cccccggccg tggtcatccc gatccggtca gcgatctggt atatcttctt tgctttcttt
     3121 gaggcgatca ggtaccccat cgtggccctc atttctgatg cgaggaccac gccatcctgg
     3181 aacaccagcc cgacggtggt ggtgcccttc attccttcct ggtatctctc attcattcac
     3241 acaccccaaa atattcgtaa acgccagtaa aatgctcgaa aagcgcttcc aaacaataca
     3301 atgtccttat aaaatttaaa agttgttatc agagatcctt ttggcagagg gcgcggataa
     3361 gatccctgtc aaagagcatg gcgaccagtt tcttgtcccc gttcacgacg gggagctggt
     3421 ccacgcgcgc ccgtttcatc ttcagggcgc actcgctcac ctcggcattc atgggcaccg
     3481 agatcacgtt cctgaccatg gccgtcttga ccgggcgatc ggggagctgg atcttggaga
     3541 tgccgtaact gatggtgtgc atgtcccgga tactctccca ggtccactcg tcatcgtcgg
     3601 tcccgttcga gaaatcgctg acttccacca tgtcctctat cgatgagttc cgaatcaggt
     3661 cacgctcgga gatgattccc tggagcgtgc tctcggcatc caggataggg atcgcctcga
     3721 acccggagat ctccatgatc cttcctacca ggggaagggg ggtctcttcc cagatggcaa
     3781 aggtctggct ggtgtaaagg tcctttatct cgtcctttat cttcatctgg gcaagggcgg
     3841 cgatgaggtc cgcaacggag agcaggccaa cgagggaccc gtcgtctatg accggcagtc
     3901 gccggaccga atgcctgacc atcatggagg ctgcttcccg gactgttgaa tcggggccga
     3961 tggtgacggg gttgggggtc atcaggagac cgagctgggt ctcttcagcc tttcggagaa
     4021 ggtctttcct tgtgatgatt cccacgagat ctgcatcctt cagcaccggt accccggata
     4081 tgccggtccg cttgaggatc ttcaggacat catcgcggtt tccgggtatc tccacggtca
     4141 ccacgtctga tgtcatatag tctttgacga tcatttctgg gatggtctca ccaccaggac
     4201 cgcaacgctg ctgttcccca ccacgaacga gctcacgctt cccaggagga gccggtcaac
     4261 ctcgctcttc ccatgtgagc ccacgacgat caggtctgcg ccaagttccc gtgatgcctt
     4321 gatgatctca tttcctgcgt ggccctgcct gatgtaggtc tcgagcctga caccctttgc
     4381 ggcagcctgc tcttttgcgt cggagattgt ctcctgcccc tctttctcga gcatgctgta
     4441 catgatctcc cacgtgctgt ccatcgggag cgaggagaac aaccccgtct ccacgacgta
     4501 cacgacatgg accctggcct tccatagccg ggcttcctcg atcgcgacct caagcgccct
     4561 ctttccgatt ttagacccgt ctactgctac caatatctcc gagaacatgg ttaccctcca
     4621 aaacagtctc cttaattaat gtcatctata aatttataac ctttcttacg atattttccg
     4681 ggcccgcccg gtttaccagg gttcctacca gatcgcggct gatcgcaagg ttcgacttgc
     4741 ctggatcaat gacgaggaaa ttcgccttct taccttcttt caggaaaaat ggttcgcaaa
     4801 agagcgacga accgagcgtt gcggatttca gcgcgtcgcg gggggtgaca cgatagacaa
     4861 aagagaggaa gtgggcctcc ctccacatgt cgggctgcac gcacatggca ttgtcggtgc
     4921 caaggagtat gtggcatccc agtgcctgca tcgtctcaat cggcgggtga tcgcacgatg
     4981 atgtgacgcc gaacctccag ttcgcccttg gacagactgc gacgggaata ccaaggtcgg
     5041 caatcctgcg aagctgggaa cgggttgcgt gcgtgcaatg gatgaggaga tcggggtggt
     5101 actcgagggc ctcgtccaca tccccggcat ccctctctcc ggcatggaat gcgaccagct
     5161 tcccggatcg cttcgcggcg gagactgcct cttccaggcc tgctcttgcg tccctggcgc
     5221 tgctgatccc gagcccgtcg gcgattgcct cgcctccttc cctcccgaaa ataatcgtcc
     5281 tgcatgggac acccctcgct gcctcccgga gtgccgatac cccgtcttcc ccgccctccc
     5341 tgaaatctgc acagccggca gttccggatt cggccatggt gaggagggtg aggtgcatcc
     5401 cggcaaggag gtctttcctg gcggcagaac ggaggatccg gtgcttgagg ccgttgggag
     5461 gccggactac ctcgtcgagt gtgccctcga agggaaggtc catggccagg ctgtcgccga
     5521 ggtgggtatg ggcattgaag aaagcggggc atatccacgg agcgttcgca ggagtggacg
     5581 ac
//
LOCUS       DAOQVJ010000036         5555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16104_length_5555_cov_3.954545, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000036 DAOQVJ010000000
VERSION     DAOQVJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16104_length_5555_cov_3.954545"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKL26_01385"
     CDS             <1..215
                     /locus_tag="PKL26_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthase"
                     /protein_id="HNS82377.1"
                     /translation="NALRAEVRSMLNLYAWDHPGTRHALANLGEELSRLGAVHGDMLH
                     TCEECGEPCGDVCRSCQILKEVAHGA"
     gene            325..1071
                     /locus_tag="PKL26_01390"
     CDS             325..1071
                     /locus_tag="PKL26_01390"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNS82378.1"
                     /translation="MEQETGCALGRIEQLIRHAYWSSGASGIVIGLSGGIDSAVAATM
                     CCRAVGGERVRALVLPSSVTLREDIEDAHEICRRLHMECQVISIDPVLDSFRAMPGYT
                     ESPYLLGNLMARTRMAILYYHANRDRRLVCGTSNRTEYLLGYSTKFGDSAADIQPIIH
                     LYKTEVCALAETMDLPERVMRKPPSAGLWPGQRDEEEIGLTYSEIDTALAALSRSGWK
                     SRTPVEEKVLGMVRASEHKRLPPPGLIPPD"
     gene            complement(1055..1753)
                     /locus_tag="PKL26_01395"
     CDS             complement(1055..1753)
                     /locus_tag="PKL26_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HNS82379.1"
                     /translation="MENWREIAGTPELRYAEDLRAVLQDRECHCRGPVYSMYRDLAAT
                     TKDREWMASLAIRFDITCIPPATLCGEWVKTKGHYHPENFAGLGYPEIYQVFEGEAHY
                     LLQKRDLSEVILVEAGEGDTVVVPPGFGHVTINPGQRELVMANLVSGEFSSEYGFYEA
                     HQGAAYYELSDGRLEKNPQYPSLPHPIRVQAAAFLPGSPFRGPLYDLVMERDRRLECL
                     NHPERFGEFFSRAG"
     gene            complement(1753..>2637)
                     /locus_tag="PKL26_01400"
     CDS             complement(1753..>2637)
                     /locus_tag="PKL26_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HNS82380.1"
                     /translation="PLQVHYVYLKTAEQFGQDAISYTYRDVDETLDLPIRIKPRAMLE
                     AVSISSEHLNAGTEGYLQIAIRNRGSEDAKDAVVRIARNGNSPVIPTDSSLFVGEFPA
                     GSSREARFKVSVSRDAGEGDYPIDLSCQYTNSEGDVVTSEILTIGVPVGGKIQFSVVS
                     SPAEVHAGRKAVIEVEYQNTGSALARHAQARISAVDPFTSNDDTAYLGDLAPGEKAVA
                     RYEVNVDPDATRKEYGLDSEIRYRDSLDNSAISDTMKVRVRVMEGENPYLLVAMVLVI
                     LAFAGGAGYYILKRRKEQ"
     assembly_gap    2638..2737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2945..3718
                     /locus_tag="PKL26_01405"
     CDS             2945..3718
                     /locus_tag="PKL26_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82381.1"
                     /translation="MAETPPSHLSLTESLKALGLTKYEALVYIGLLKVSGATATDIHE
                     ISGVPRASVYPVLDRLIQKNLVSVSHSTPKRFEAVPPEDGIDHLLELVESDAARARKM
                     LSRIYRDRSSGERGDQELIWSIYGDEHIRSRLIDLLQHAECSIDTIFFGGFLREEITA
                     ALIAKSGAVPIRVVTDRWDVPLPGKISVHVKSPPESLKNLKPSSIAGGVFLIDQRRAM
                     VVMVSGGEGPTALYSESKGFVKFFSLYWELLSSCEAVLA"
     assembly_gap    3799..3898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3899..4299)
                     /locus_tag="PKL26_01410"
     CDS             complement(<3899..4299)
                     /locus_tag="PKL26_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNS82382.1"
                     /translation="MNLPLKFVHTRIEEYAMQVTFDPREGRGTVVYNLSLIRKEDLEY
                     AISVMKDAFKAGICVSGLVRFIEEGATVGDFQVPSGHCAVITMCSSTLDGILLRKGVP
                     SNPIGGGVVEIESRVPRRFTHMILYEHTTIDP"
     gene            4365..5336
                     /locus_tag="PKL26_01415"
     CDS             4365..5336
                     /locus_tag="PKL26_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82383.1"
                     /translation="MDEKVREKARNAIKSILEAAGFSVNTLEDPFDLAERDDEYLMVL
                     CSNDPGEIEEYNTGAYRLMKDGEGITYKKLLFSLEPSATAGNCILWRSDEFIRYAGEA
                     ALDEILGRPLALSFSDGSRTKADAGPALMRGRSTQAIEEEEPGGIVISHLPLRVQKQA
                     AEQIAKVQGQASLKFMPYWLYSYQSSGEQVYRDQRIGFDSEGSGGINAINGTKMDADW
                     NAVAESAIPQSAEVVQPRLTREEATEKVVAEVIESLTRKIRIKQVIGDAISYEEKVMR
                     PDRKNVQVQLQQAFIPVWQVRGKKIVEVNAYTGEVLSEPMDEGVEVF"
     gene            5349..>5555
                     /locus_tag="PKL26_01420"
     CDS             5349..>5555
                     /locus_tag="PKL26_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS82384.1"
                     /translation="MIAVLGGGPAGRIAAMQLGLAGREVTLVEKGGLGGQCLHHGCMV
                     VCALNDIARHLFFSRDLQSLGILDS"
BASE COUNT         1091 a         1568 c         1592 g         1104 t
ORIGIN      
        1 acaatgccct cagggcagaa gtccggagca tgctcaatct ctatgcctgg gatcatcccg
       61 gcacccgcca tgccctggcc aaccttgggg aggaactctc caggcttggt gcagtgcacg
      121 gcgatatgct gcacacctgt gaggagtgcg gggagccctg tggtgacgtg tgccgctcct
      181 gccagatact caaagaggtg gcccatggtg catgaatcat ccggcatctt tcggcacctt
      241 gctcatcctc ccggcgtcaa cacatgggac caatgtgaaa accggggttc atcaaagacg
      301 aaaaaaaggg acgtgtgata ctgcatggaa caggaaacgg ggtgtgcact gggaaggata
      361 gaacagctga tccggcatgc atactggagc agcggggcca gtgggatcgt gatcggactc
      421 tcagggggaa tcgactcagc agtggccgcg accatgtgct gccgggcggt gggtggggag
      481 cgggtgcgtg ccctcgtcct cccaagcagc gtcaccctcc gcgaagacat cgaggacgcc
      541 cacgagatct gcaggcgcct ccacatggaa tgccaggtca tcagcatcga tcccgtcctc
      601 gattcgttcc gtgcaatgcc ggggtatacc gagagcccgt acctgctcgg gaacctgatg
      661 gcaaggaccc gcatggcgat cctttattac cacgccaacc gcgacaggag gcttgtctgc
      721 gggacctcca accggaccga gtatctcctc ggctactcga ccaagttcgg ggacagcgcc
      781 gccgatatcc agccgatcat ccacctctac aagaccgagg tctgtgcgct cgctgagacc
      841 atggacctgc ctgaacgggt gatgaggaag cctccctcgg cagggctctg gcccgggcag
      901 cgggacgagg aggagatcgg gctcacgtac agtgagatcg atacggcgct ggccgctctc
      961 tcaaggtcag ggtggaagag ccgtaccccg gtggaggaga aggtgcttgg aatggtcagg
     1021 gcaagcgagc acaagcgcct ccccccgccg ggtctcatcc cgcccgactg aagaactccc
     1081 cgaaccgttc ggggtggttg agacattcca gtcggcgatc cctctccatt accaggtcgt
     1141 agagcgggcc cctgaaaggg gatcccggca ggaatgctgc tgcctgtacc cggatgggat
     1201 gagggagtga aggatactgg ggattcttct ctaacctgcc atcggatagt tcgtaatacg
     1261 cagctccctg gtgcgcctcg taaaatccgt attcacttga aaattcgcct gatacgaggt
     1321 ttgccatcac cagctcgcgc tgcccggggt tgattgtcac atggccaaag ccagggggca
     1381 cgacgactgt atcgccttct cctgcctcca ccaggatgac ttccgagagg tcccgcttct
     1441 gaaggaggta atgggcctcc ccttcgaata cctggtagat ctccgggtat ccaagtcccg
     1501 caaagttctc ggggtgataa tgacccttgg tcttcaccca ctccccacac agggttgcag
     1561 gagggatgca ggtgatatca aaacggatcg cgagggaggc catccactcc cggtctttcg
     1621 tggtggctgc aaggtcccgg tacatcgagt agaccgggcc tctgcaatga cactccctgt
     1681 cctgcaggac ggccctgaga tcttcagcgt agcgcagttc tggggtccct gcaatctccc
     1741 gccagttctc catcactgtt cctttcgtct cttcaggata taatatcccg ctccgcctgc
     1801 gaaggcaagg ataacgagga ccattgcgac cagaaggtat gggttttctc cttccatgac
     1861 cctgacccgc accttcatcg tgtctgagat cgcgctgtta tccagggagt cgcggtagcg
     1921 gatctcggag tcgaggccat actcctttcg ggtggcatcc gggtctacgt ttacttcgta
     1981 cctggcgacc gccttttcgc cgggggccag gtcgccgagg taggcagtat catcgttgct
     2041 ggtgaagggg tcaaccgcac tgatccgggc ctgtgcatgc ctggccagtg ctgatcccgt
     2101 gttctggtac tccacttcta tgaccgcctt tctccctgca tgtacctcgg ccggggagga
     2161 gacgaccgag aactgtatct ttcctccgac agggacaccg atggtgagga tttccgatgt
     2221 gaccacgtcc ccctcactgt tggtatactg gcaggagaga tcgatcggat agtccccctc
     2281 gccagcgtca cgggagaccg agaccttgaa tcgtgcctcc ctgctgcttc ctgcagggaa
     2341 ctcccccacg aacaggctgc tgtcggtcgg gatcacgggg ctgttgccgt tccttgcaat
     2401 cctgaccacc gcatccttcg catcctcgga cccccggttc cgtatagcga tctgcaggta
     2461 cccttccgtt ccggcattga ggtgctcgct gctgatggag accgcctcga gcatcgccct
     2521 cggctttatc cggatgggaa ggtcaagtgt ctcgtcaaca tccctgtagg tataggagat
     2581 ggcgtcctgc ccgaactgtt ccgcggtctt caggtacacg tagtggacct gcaggggnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc ctcgccaggg gagaactcgt
     2761 ttcttccggc cacggacgcg gagaggtccg ggtccccggt caggtacttg acatccgcca
     2821 tgaccggcgc tccaattaaa ctaaataaaa tggccagaat cacgaaatga cgcatattca
     2881 tgtgaaacca cttgttgtta ttgctataac aacatttaag attcgccata tataatggta
     2941 tgtaatggcc gaaacacccc catcccatct ctccctcacg gaatccctaa aagccctcgg
     3001 gctcaccaag tacgaagcgc tggtgtatat cggtctcctc aaggtctctg gagcgactgc
     3061 caccgatatc cacgagatct caggcgttcc ccgtgcctcg gtctatcctg tgctggaccg
     3121 cctcatccag aagaacctgg tctcggtctc ccactccacc cccaagcggt tcgaggccgt
     3181 tccccctgag gacggaattg atcacctgct ggagttggtc gagtcggatg ccgcacgagc
     3241 gaggaagatg ctctcccgga tctacaggga caggtcatca ggtgagcgtg gagaccagga
     3301 gctgatatgg agcatatacg gcgacgagca catccgctcc cgcctgatcg acctcctcca
     3361 gcatgcggag tgctccatcg acaccatatt ttttggcgga ttccttcgcg aagagattac
     3421 cgcggccctg atcgcaaaga gcggggccgt ccccatccgg gtggtgacgg accggtggga
     3481 cgttcctctc cccgggaaga tctcggtgca cgtgaagtcc ccgccggagt ccctgaaaaa
     3541 tctcaagccc tcgtctattg ccgggggagt gtttctcatt gaccagagga gggcaatggt
     3601 ggtcatggtc tcggggggag aggggccgac tgcgctctat tctgagtcaa aaggattcgt
     3661 gaagttcttc tcgctttact gggagctcct ctcctcgtgc gaggcagttc ttgcctaaaa
     3721 ctccaggatc tcctgcatct cctggacgtc catcagcccc ttgatcgcct gggtcacgac
     3781 atagacgccc ccttccttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     3901 gtcgatggtg gtatgttcgt acaggatcat gtgggtgaac cgccttggga cacggctctc
     3961 gatctcgacc accccacccc cgatggggtt cgagggcacg cctttcctga gaaggattcc
     4021 gtcaagcgtg gagctgcaca tagtgatgac ggcacaatgg cccgatggca cctggaaatc
     4081 cccgactgtt gccccctcct cgatgaaacg cacgaggccg ctcacgcaaa tccctgcctt
     4141 gaacgcgtcc ttcatgacag agatggcgta ttcgaggtcc tctttcctga tgagcgagag
     4201 gttgtagacg acagtccctc ttccttcccg gggatcgaag gtcacctgca tggcgtactc
     4261 ctctatccgg gtatgcacga acttgagggg gaggttcatc tctccatcta atctctcctg
     4321 tcaatagaaa aattgttcta taagatggag gaaatgtatg gttaatggac gaaaaggttc
     4381 gggaaaaggc gagaaatgcg ataaagtcca tcctggaggc ggcagggttc tcggtgaaca
     4441 cgctcgagga tccctttgac ctggccgagc gggatgatga gtacctcatg gtcctgtgca
     4501 gcaatgaccc gggagagatc gaggagtata acaccggtgc ataccgcctg atgaaggacg
     4561 gcgagggtat cacctataag aagctcctct tctccctcga gccttctgcc actgcgggga
     4621 actgcattct ctggaggagc gacgagttca tccggtacgc gggagaggcg gccctggatg
     4681 agatccttgg acgccccctc gcactctcgt tttcggatgg gtcgcgcacg aaggcggatg
     4741 cggggcccgc gctcatgagg ggccgcagca cccaggccat agaagaggaa gagcccgggg
     4801 ggattgtcat ctcccacctc cccttgcggg tgcagaagca ggctgccgag cagatcgcga
     4861 aggtccaggg gcaggcaagc ctgaagttca tgccatactg gctctactca taccagagtt
     4921 caggggaaca ggtgtacagg gaccagcgca tcgggtttga ttccgagggg agcggcggga
     4981 tcaatgccat caacgggacg aagatggatg ccgactggaa tgccgtcgca gaatctgcca
     5041 tcccccagag tgccgaggtt gtgcagcccc gtctcaccag ggaagaggct accgagaagg
     5101 tcgttgcaga ggtgatcgag agcctcaccc ggaagatccg gatcaagcag gtgatcggcg
     5161 atgccatctc gtacgaggag aaggtgatga gaccagacag gaagaacgtc caggtgcagt
     5221 tgcagcaggc atttatccct gtctggcagg tgcggggcaa gaagatcgtc gaagtgaatg
     5281 cctatacggg cgaggtcctt tccgagccca tggacgaggg cgtcgaggtc ttctgatatg
     5341 gccggtccat gatcgcagtt ctcggcggag gccccgcggg caggatcgca gccatgcagc
     5401 ttggcctcgc aggaagagag gtcacccttg ttgagaaggg ggggctcggg gggcagtgcc
     5461 tccaccatgg ctgcatggtg gtatgcgcat tgaatgacat cgccaggcac ctcttcttct
     5521 ccagggacct tcaatctctt ggcatcctcg actct
//
LOCUS       DAOQVJ010000037         5554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16108_length_5554_cov_3.985452, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000037 DAOQVJ010000000
VERSION     DAOQVJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16108_length_5554_cov_3.985452"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKL26_01425"
     CDS             complement(<1..513)
                     /locus_tag="PKL26_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNS82385.1"
                     /translation="MEDPLRRALLLFALQNAVKHQSIPKAGTVIGMVMGKYPELKPRA
                     REATTLIKEVIDEVTALTPAERADRLCSLAPELVEEMSRPREHAKALPTLEKAENGVV
                     MRFAPNPSGPLHLGHARAAVLNDAYVRRYGGRYVLRIEDTDPKRVDPHAYAMVKEDID
                     WLGLAISDIVY"
     gene            complement(515..1477)
                     /locus_tag="PKL26_01430"
     CDS             complement(515..1477)
                     /locus_tag="PKL26_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS82386.1"
                     /translation="MELNSYLEQTANQVDSLIHRYFGSSCDDLGRASAHLLLAGGKRL
                     RPALLLLSADAVNRGSAVDVVPAALALELTHSFTLIHDDIMDGDLVRRGVPTVHTQWD
                     EPTAILAGDVLFASAFEFLSLADAPDNAKVRATSMLARTCVEICEGQHMDMAFEKRDD
                     VSEGEYMKMVEKKTGALYAAAAGIGSILAGGNPNFTDALYHFGQGIGMAFQIQDDLID
                     LLASPEVSGKDQASDIREGKKTLIHIKALEKGFDLSQFQRRLSQAEIQEIITRLRGIG
                     VISEVQDTARNLVKTGQKRISILPESEERDLLIHMGDHFISRSY"
     gene            complement(1499..2836)
                     /locus_tag="PKL26_01435"
     CDS             complement(1499..2836)
                     /locus_tag="PKL26_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HNS82387.1"
                     /translation="MDIEIIAVGGYDEVGRNMTAVRCGKDIVIFDMGLRLDQVMIHEE
                     AEVENMHSLDLIQMKAIPDDTMMNTVEGSVKAIVCSHGHLDHIGAIPKLAHRYNAPII
                     STPYTTELIRQQIAGEQKFGVNNKLFALRAGQRYTLSQQLTLEFVRMQHSIIDTVTAV
                     LHTPKGAIVYACDYKLDRTPVIGEPPDFARLRQIGKEGVLALIVEATYIDNRGRAPSE
                     RIARDLVRDTITSYEDDKSAIIVSTFSSHIARVKTIAECAHEIGRKPVLLGRSMEKYS
                     STAEQMKLVSYPDTMSMFGNRRTVDRTLRRMMKAGKEKFVPIITGHQGEPGSILTRIA
                     LGDTPYKVEKGDKVIFSAKVIPNPMNVGQRYMVEAHLNMAGARIFPDLHVTGHAYRED
                     HYEFIHLLNPQHIIPAHGHIRMTSGYAEFTEAIGYTLHNDVHILSNGQRVKMT"
     gene            complement(2836..3915)
                     /gene="fni"
                     /locus_tag="PKL26_01440"
     CDS             complement(2836..3915)
                     /gene="fni"
                     /locus_tag="PKL26_01440"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNS82388.1"
                     /translation="MDKTPLTSGRKLDHLRICADEEIERGSPGFEDVRLVHSALPECD
                     LSRIDLSTRFLGQRLNSPLFIAAMTGGHPGTTEVNRCLARAAERYGLGMCVGSQRAAL
                     ENPRLEESFRVVREEAPHAFLSANLGIVQLRDHGLDWAERAVSMIDADAISIHLNFLQ
                     EAIQPEGDHDATGCYDALAALCMESKVPVIVKETGSGISAETARACWGAGVRAIDIGG
                     WGGTSWAAVEAFRAERSPGHQENRLISLGALFEDWGIPTVVSLCEVASTGSPVIATGG
                     IRSGLDMARALALGADLCGVALPLLKTALESEDTLFTRIETLHRELSTAMFLSGSAKI
                     ADQKRARAYITGKTRQMLEHIQGGK"
     gene            complement(3908..4678)
                     /locus_tag="PKL26_01445"
     CDS             complement(3908..4678)
                     /locus_tag="PKL26_01445"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNS82389.1"
                     /translation="MSERLLLKIGGSVLTEKGRQGAADTRAIATIASEIAGHAESSLC
                     LVHGAGSFGHPEAHRYGLAKGADRNNRQGIALTHGAVCRLNQMMVEALRDAGADAIGI
                     HPLGSAWARAGRLLTLETRPLQRLMDVGIIPVLHGDVVMDEEQGACIVSGDQIIRYLG
                     GHLAFTRIGLATDVPGVLTPEGGVINEIPAGVSPEALAGSSGHMDVTGGMAGKVRELQ
                     ELASLGVPSEIFHISRLSDFMDSRPCGGTRVGGGRLRG"
     gene            complement(4671..5540)
                     /gene="mvk"
                     /locus_tag="PKL26_01450"
     CDS             complement(4671..5540)
                     /gene="mvk"
                     /locus_tag="PKL26_01450"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNS82390.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGIAMAIKPRVFVTVRKSRMAH
                     HARSPYIDSCFDEMGVKGSVYVNSQLPSSSGLGSSAAVTVATLSAINDEFGKKYSMEE
                     IATMAYAIEKKVQKGKASPTDTSVSTFGGIVMITGTQRRRLPPQNLPLVVGNTLVSHS
                     TAKMVEKVADLKKKNPEICEPILDAIGAVSMCAIRNFSDPHALGNLMNMNHALLEALG
                     AGHPTLSRLVLAARAAGAYGAKLTGAGGGGCMVAICPKHLKSRVVGAVEACDAKAFIT
                     NLDTEGARKEKDV"
BASE COUNT         1014 a         1750 c         1551 g         1239 t
ORIGIN      
        1 gtacacgatg tcgctgattg caagaccaag ccaatcgata tcctccttca ccattgcgta
       61 tgcatgaggg tccacccttt ttggatcggt gtcctcgatc ctgaggacgt agcgcccgcc
      121 atagcgacgg acataggcat cgttcaagac cgccgccctt gcgtgcccga ggtgaagggg
      181 gccactcgga ttgggggcaa acctcatcac gactccattc tcggccttct caagggtggg
      241 cagcgccttc gcatgctcgc gggggcgcga catctcctcc acgagttccg gcgcaagaga
      301 acaaaggcga tcagcccgtt ccgcaggggt gagcgcggtg acctcgtcga tgacctcctt
      361 tataagtgtc gtcgcctccc tggcccgggg tttcagttca gggtacttcc ccatcaccat
      421 cccgatcaca gtcccagctt tcgggatgct ctggtgcttc actgcgttct gcagtgcgaa
      481 gaggaggagc gctctcctca acgggtcctc catctcaata gctccggctg atgaagtggt
      541 cacccatatg gatgagaagg tcgcgctcct cgctctctgg gaggatggat atgcgctttt
      601 ggccggtctt caccaggttc cgggcggtat cctgcacctc cgagatcaca cctatcccgc
      661 ggagacgcgt gataatctcc tggatctcgg cctgcgagag ccttctttgg aattgactga
      721 gatcgaaccc cttctccagc gccttgatat ggatcagggt ctttttcccc tcccggatgt
      781 cagatgcctg gtccttcccg cttacctccg gggacgcgag aaggtcgatg aggtcgtcct
      841 ggatctggaa tgccatcccg ataccctgcc caaagtggta gagggcatcg gtgaaattcg
      901 ggtttccccc ggcaaggata cttccgatgc cggctgcagc cgcgtacagc gcaccagtct
      961 tcttctccac catcttcatg tattcaccct cgctcacatc gtcccgcttc tcgaaggcca
     1021 tgtccatgtg ctggccctcg cagatctcca cgcaggtccg ggcaagcatc gaggtagccc
     1081 tgaccttagc attgtcgggg gcgtctgcaa ggctgagaaa ttcaaatgcg ctcgcaaaga
     1141 gcacatctcc cgccaggata gcggtgggct cgtcccactg cgtgtgcacg gtgggcactc
     1201 cccttctcac caggtcgccg tccatgatgt cgtcatggat gagggtgaaa ctgtgggtga
     1261 gctcgagcgc gagtgccgcg gggaccacgt ccacggcgct ccccctgttc accgcatctg
     1321 cggagagcaa cagcaatgcc gggcggagcc tcttgcctcc cgcaagaagc aggtgcgcgc
     1381 ttgcccttcc caggtcatcg catgacgatc cgaaataccg gtgaataagg gagtcaacct
     1441 gattggcggt ctgctccagg taactattca gctccatctc ctcctcgtcc tccttgcctt
     1501 atgtcatctt cacccgctgg ccgtttgaca ggatatgcac atcgttgtga agcgtatacc
     1561 ctatcgcctc ggtgaactcc gcgtatcctg atgtcatccg gatgtgcccg tgggccggga
     1621 tgatatgctg ggggttcagg aggtggataa attcgtagtg atcctcccgg tatgcatgtc
     1681 ctgtcacgtg aaggtccggg aagatgcgtg ctcctgccat gttcaggtgt gcctcgacca
     1741 tgtaacgctg cccgacattc atcgggtttg ggatcacctt ggcgctgaag atcaccttgt
     1801 cgcccttctc caccttgtag ggggtgtctc caagcgcgat ccttgtcagg atggacccgg
     1861 gctcaccctg gtggccggtg atgatgggga caaacttctc cttccccgcc ttcatcatcc
     1921 tccgcagtgt gcggtcaact gtcctcctgt tcccgaacat gctcatggta tcagggtagc
     1981 tgacaagctt catctgctcg gcggtggacg agtatttctc catggacctg ccgaggagga
     2041 ccggttttcg tccgatctcg tgggcgcact ctgcgattgt cttcaccctt gcaatgtgcg
     2101 aggagaacgt ggagactatg attgcagact tgtcgtcttc gtaactcgtg attgtgtccc
     2161 tgacaagatc ccttgcgatg cgctcgctcg gggcacgacc cctgttgtcg atataggtcg
     2221 cctccacgat aagggcgagc actccctcct tcccgatctg ccgcaggcgg gcaaagtcgg
     2281 ggggctcacc aataaccggt gtcctgtcga gcttgtaatc acaggcatac acaatggcac
     2341 ctttcggcgt gtgaagcacc gcggtcaccg tatcgatgat gctgtgctgc atgcgtacga
     2401 attcaagcgt caattgctgg gacagggtgt atctctggcc ggctctcagg gcaaacaact
     2461 tgttgttcac cccgaacttc tgctctccgg caatctgctg ccgtatcagt tcagtggtgt
     2521 agggggtgct gatgatcggt gcattgtagc ggtgggcaag cttcgggatg gccccgatat
     2581 ggtcaaggtg cccgtgagaa cagacaatgg ccttgacact tccctccacc gtgttcatca
     2641 tggtgtcgtc cgggattgcc ttcatctgga taaggtccag ggagtgcatg ttctcgactt
     2701 ccgcctcctc gtggatcatc acctggtcca gacggagccc catgtcaaaa ataacaatgt
     2761 cctttccgca gcggacggcg gtcatatttc tcccgacctc gtcgtacccg cccaccgcga
     2821 ttatttcaat atccattatt ttcctccttg tatgtgttcc agcatctgcc gggtcttccc
     2881 ggttatatac gcccgggcgc gtttctggtc cgcgattttt gcagaccctg agagaaacat
     2941 tgctgtgctc agttccctgt gaagtgtctc gatccgggtg aagagtgtgt cctcactctc
     3001 cagtgccgtt ttgagcaggg gcagggcgac gccgcacaga tccgcaccga gtgccagtgc
     3061 acgggccatg tcgagcccac ttctgatgcc ccctgttgcg atcacgggac tgccggtact
     3121 ggctacctcg cagaggctca cgacggtcgg aatcccccaa tcctcgaaca gggcgccaag
     3181 agagatgaga cggttctcct gatgcccggg agatcgctct gcccggaacg cttctacggc
     3241 cgcccaggaa gttccacccc atcctccgat gtctatggcc cggactcctg ccccccagca
     3301 tgcccgcgca gtctcggccg agatgccgct tccggtctcc ttgacaatca ccgggacctt
     3361 gctctccatg cagagcgccg cgagggcatc gtaacaaccc gtggcatcat ggtcgccttc
     3421 gggctggatc gcctcctgaa gaaaattcag gtgaattgaa atggcatcgg cgtcgatcat
     3481 cgacaccgcc cgctccgccc aatccagccc atggtcgcga agctgtacaa tccccaggtt
     3541 tgcagagaga aatgcatgcg gtgcctcctc ccgcaccacc ctgaagctct cctcgaggcg
     3601 cgggttctca agcgcggccc tctgggaccc gacgcacatg ccgaggccat accgttcggc
     3661 agcccttgca aggcagcggt ttacctctgt ggtgccgggg tgaccaccgg tcatcgcagc
     3721 aatgaagagg ggagaattga gacgctgtcc cagaaacctt gtggagaggt cgatccggga
     3781 aaggtcacat tccgggagtg cagagtggac aagccgcacg tcctcgaatc ccggggaacc
     3841 gcgctcgatc tcttcatctg cacagatacg gaggtgatcg agcttcctgc ccgatgtaag
     3901 cggagtctta tccacgaagc ctgccacccc ctaccctcgt cccgccgcat ggtcgtgagt
     3961 ccatgaaatc cgatagcctg gagatgtgaa agatttcgga aggtactcca agggacgcaa
     4021 gctcctggag ctccctgacc tttcctgcca ttcctcccgt cacgtccatg tgaccggagc
     4081 ttccggccag tgcttccggg gatacccctg caggtatctc attgattact cctccttcag
     4141 gagtgagcac gccggggaca tcggtggcaa gtccaatcct cgtgaatgca agatgcccgc
     4201 ccaggtaacg gatgatctgg tcgccggaca cgatacaggc gccctgctcc tcgtccatta
     4261 cgacatcccc gtggagtacc ggtatgattc ccacgtccat caggcgttgc agaggccggg
     4321 tctcaagagt gagcaggcgc cccgccctgg cccacgcgct gccgagggga tgaatgccaa
     4381 tcgcatcagc ccctgcatcc cgcagggcct ccaccatcat ctggttcagc cggcagacag
     4441 ccccgtgtgt cagggctatc ccctgccggt tgttccggtc cgctcccttc gccagcccgt
     4501 acctgtgggc ctcggggtgg ccaaatgacc ccgcgccgtg aacgaggcag agggaactct
     4561 ctgcatgtcc tgcgatctct gacgcaatcg tcgcaatagc ccgggtatcg gcggctccct
     4621 gcctgccctt ctccgtcaac acgctgcccc cgatcttgag cagcagtcgt tcagacatcc
     4681 ttctccttcc ttgccccttc tgtatcgagg ttggtgatga atgccttggc atcacatgcc
     4741 tcgacagccc ccaccaccct gctcttcagg tgcttcgggc agatggcaac catgcaaccg
     4801 cctcctcccg cacccgtcag ctttgccccg tacgcgcctg ctgccctcgc agcaaggacc
     4861 aggcgggaga gcgtggggtg tcctgcacca agggcctcga ggagtgcatg gttcatgttc
     4921 atcaaattcc caagtgcgtg gggatctgaa aaattgcgga ttgcgcacat cgagacagcc
     4981 ccgattgcat cgagtatcgg ttcgcagatc tcgggatttt tcttcttgag gtccgcgacc
     5041 ttctcgacca tcttcgcggt gctgtgtgag acgagggtgt ttcccaccac cagggggaga
     5101 ttctggggag gtagcctccg gcgctgggtg cccgtgatca ttacaatgcc accgaaggtg
     5161 gagacgctcg tgtcggtggg gcttgccttt cccttctgga ctttcttctc gatggcgtac
     5221 gccatggtcg ctatctcctc catgctgtac ttcttcccga attcgtcgtt gattgccgag
     5281 agggttgcaa cggtcacggc agccgaggaa ccgaggcccg aggatgaagg gagctgggag
     5341 ttcacgtaca cgcttccctt gacccccatc tcgtcaaagc agctgtcaat gtagggggac
     5401 cttgcatgat gggccatccg gctctttcgc acggtgacaa agaccctagg cttgattgcc
     5461 atggcgatcc ctggctttcc atacaccaca gcatgctcac cgaacaggaa aaccttgcca
     5521 ggcgcactcc acgtggccac cctcagatca ccgc
//
LOCUS       DAOQVJ010000038         5529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16199_length_5529_cov_4.321520, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000038 DAOQVJ010000000
VERSION     DAOQVJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16199_length_5529_cov_4.321520"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKL26_01455"
     CDS             <1..229
                     /locus_tag="PKL26_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82391.1"
                     /translation="RGRSDRPCFVPPDVDEVGAFLGEVVEGPILPVAHWDLEAEERDR
                     DLALERARDTSAISFSGIDCARETMSVQHNI"
     gene            285..5072
                     /locus_tag="PKL26_01460"
     CDS             285..5072
                     /locus_tag="PKL26_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS82392.1"
                     /translation="MDSTYLQEIQVIIQKDIIQSTYKLALLRAIIEIAQESNGSKVVL
                     NGMCAYPFSLLQRKLLTYYYPLFAHPSFIPQMRRESPVSRNGRQLVIRTSMNPIIQYY
                     HSNGGYEGLLSDLEKNHIPGEIATDYSRFLETIRRTFIAQPMKYLGNSIRPEGYTVVK
                     YLTRLPPNGSMLLTSPADLEGYYAISQEYARIFEEPSCAQHLLDLVVRRWIDYTLSLS
                     DTLTHEELYGLFAPVSHTGIDVIELHPSPKPAISLPLPAPISTRFEKSISSRENPANP
                     ASMILALEEAIRAEYSQFSSQQQSIQDGTDAVLKAELRSQKIDESIRRIEALYGISSQ
                     GSVLQGPFEVLSHYYADQQAAQDAYSGTMTHLLASKRKLSELKRERDRLQQMAQKVTR
                     SLTVRKRQQDLIDRLHHELALLDDGSDVQPRPFGYIDKPIARFLTYCGDVTLDILGQC
                     IRLIPDPEETTMVKPALPAWFIDEFDTWRIRKQEMQKSSRESGSGKSSTHRPFITFDE
                     HHREVQLTIPSQVIERSEGLDHVALIIHDGSQILHEEELPLYHADEGLATEENTVRVE
                     RPSQHYHVELSAPGVSSTWVIDGSGNSDALCLFDYETGRRIEQQRIPPKRFILLTRSE
                     PTITPEEAVLFTGRLSGDWYDFKYYDIDPVDGLVIGGPQALSGEDGREPVKLDLRIDP
                     GYFDRYLRVDGWSVIPGSPPCIQILFADKGVLSSTTLSIHPRGRDPTLKPTFHSLGEP
                     GEGVEIDPDGHICTVDLGHSNLLGRERAGAFTIRVYNESCRTDIRVECVFLPGISYRF
                     SKPLYLPAEGASPVQLDITCPSSVRFEPDGPVKMDRTEAGFLITANLVPAIRGKLWYP
                     AGDGSAFAGILSISVPHAAWRFEDEATGTIYPLRRSVPVIPDIDYRSLGTEPGLHVFL
                     PESFSGTGTISVLPQNQTVTCRVVKGQGYFSLARFNDTLRATDAKCMHFEFSMEEPRD
                     GGIRFPLFSLQRWYIHLPNPPRVSIDPSGNRSIEIEWEEYGIAPKRVLLLWRKKKSGG
                     TSRIYVEEIPPAVRKLTIQGDHDLPLSPGIYYLQFYRILNDWDLPPASFGERAPNVFP
                     LRIEQSKDRISDEQPPESGGDVPGSSELPKLDAGIERLIGEIESGDPERLTRALRDLK
                     EDYSIRRINHHLLEKYPAQYERLQAALFAVIMDARIPQEVRSTSVVLLGKSFNQEREH
                     TLITVLGSPDSAKDLRIAILKALGHGNTDECLNALLTTRDDPDPDVRVELAASLGNID
                     NKGAVKPLIALLDDESVVVQRKAAASLGYTHSHASFEPLMSIALDEKRDMVLRITAIS
                     ALAKFRLNEQTTRELFTLSKNAGEDEEVRKIATSVLSIQGRLNVEEKKRHIADLIRDV
                     KTSSHGKRLSAIKQLSAFGDEEVILALMEAAQDESVDVRRVAADSLRRINKPMAIPTL
                     IELCDDPDDDIRETAEFACRQLLEVLKSDLKQTSDKRATAAGHLSRIGALAVNPLLVM
                     LRKSESIRSERADSGEIQNSIVDALSRMNDPDAIALMIRALQDKNDLVVWGAAKALGA
                     IGDASAIGPLKEALARWYNPMRCMTPTTYVWMEIEKTLQRLNG"
     gene            complement(5209..>5529)
                     /locus_tag="PKL26_01465"
     CDS             complement(5209..>5529)
                     /locus_tag="PKL26_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNS82393.1"
                     /translation="LDKVADIICPMAYPSHYSWSRKFYADPYYTVHETSTRAKLRSKN
                     AMIVTYIQAFKMKMSGIPFDKYVLVDSSPEQGGEIGIVYLKRRLEIPHIALSDRLRCC
                     KGIQ"
BASE COUNT         1376 a         1435 c         1442 g         1276 t
ORIGIN      
        1 aaggggcagg tcagatcgcc catgcttcgt acccccggac gtggatgagg taggcgcatt
       61 tctcggcgag gtggtcgaag gcccgatcct gccagtcgct cattgggatc ttgaggccga
      121 ggagcgagac cgcgatctcg cgctcgaacg ggcgagggac acgagtgcga tctcgttttc
      181 gggcattgat tgtgcgagag agactatgag tgtccagcat aatatctaaa agtacagatc
      241 aagcctattt ataagcatat aaatcgaatg gacgtttcgt aagaatggac tctacatacc
      301 ttcaggaaat acaggttatc atccagaaag atatcatcca gtccacctac aaactcgctc
      361 tcctgcgggc aatcatcgag atcgctcagg agtctaacgg aagtaaagtc gtcctgaatg
      421 gaatgtgcgc atacccgttc tcgcttctac agcgtaaatt gcttacttat tactatcctc
      481 tatttgcgca tccatcattc atcccccaga tgcgtaggga atcaccggtt tcgcgtaatg
      541 gaagacaact cgtcattcgc acaagtatga atcctattat tcagtattat cattctaatg
      601 gtggttacga agggctcctg tcggatctgg aaaaaaatca tattcccggt gagatcgcga
      661 cagattactc tagatttctt gagacgatcc ggagaacatt cattgcacaa ccaatgaagt
      721 atcttggcaa ctcaatccgg cctgaaggat acacggtggt caagtatcta acacgactcc
      781 ctccaaatgg cagtatgctt ctgacttctc cagcagacct ggagggttat tatgccattt
      841 cgcaagaata tgcccgaatt tttgaagagc cctcatgtgc acagcatctc ctcgatctgg
      901 tggtccggcg ctggattgac tatacgctca gcctatcgga taccctcacc cacgaggagc
      961 tttatggatt gtttgcaccc gtttctcaca ccgggatcga tgtgatcgaa ctccacccct
     1021 ctccaaaacc agccatatcc ctcccgctcc ccgcgccaat ctctacaagg tttgagaaga
     1081 gcatctcttc ccgggaaaac ccggcgaacc ccgcctcgat gatactcgct cttgaggagg
     1141 ccataagggc tgaatattca cagttctcct cacagcaaca gagtatccag gacggcacgg
     1201 atgctgttct caaggcagaa ctgagatcgc aaaagatcga cgaatccatt cgacggatcg
     1261 aggctcttta tggcatatcg tctcaaggca gcgttttgca gggacctttc gaagtactct
     1321 cccactatta tgcggaccaa caggccgcgc aggacgccta ctctggcaca atgactcatc
     1381 ttctcgcaag taagcggaaa ctctccgaac ttaagcggga aagagaccgg ctccagcaga
     1441 tggctcaaaa agtgacgagg agtctcaccg tgaggaagcg gcagcaggat cttatcgacc
     1501 ggctacatca tgagcttgca ctccttgacg atgggtctga tgtccagccc agaccgtttg
     1561 gctacatcga taaaccgatc gcacggtttc tcacctattg cggtgacgtt acactggata
     1621 ttctgggcca gtgtatccgc ctcatccctg acccggaaga gacgacaatg gtaaagcctg
     1681 ccctgccggc ctggtttatt gatgagttcg acacctggcg gataagaaaa caggagatgc
     1741 agaagagtag cagggagagc ggctccggga aatcctccac gcatagaccg ttcatcacct
     1801 ttgatgaaca ccaccgtgag gttcaactga ctattccatc gcaggtcatc gagcgtagcg
     1861 aggggctcga ccacgtggca ctcatcattc acgacggttc acagatactc catgaggaag
     1921 aactcccgtt gtaccatgcg gatgaggggc tggcgacaga ggagaacaca gtgcgcgtgg
     1981 agcgtccctc acagcattac cacgtcgaac tatctgcccc gggggtatcc tcgacatggg
     2041 tgatcgacgg gtctggcaat tcagatgcct tatgcctctt cgactatgaa acgggcaggc
     2101 gtatcgaaca acaacgaatt ccaccaaaga ggttcatcct cctcacccgg agtgagccaa
     2161 caataacccc tgaagaagca gtgctcttta ccggccgcct ttctggcgac tggtatgact
     2221 tcaagtatta tgatatcgat ccggttgatg gccttgtgat aggtggtcct caagcgctct
     2281 ccggcgaaga tggtcgggaa cctgtaaaac ttgaccttcg catcgatccc gggtactttg
     2341 ataggtatct tcgggtggat gggtggagcg ttatccctgg ttctccacct tgcatccaga
     2401 ttctgtttgc ggataaagga gttctctcca gtacaaccct ctccattcat ccgcgtggtc
     2461 gggatcccac tctgaaaccg acattccatt cccttggaga accgggggag ggagtggaga
     2521 ttgatcccga tggacatatt tgtaccgtgg acctcggcca ctcgaatctg ctggggagag
     2581 aacgggcagg ggctttcacc atacgtgtat ataacgagag ttgtcgaacc gatatcagag
     2641 tagagtgtgt gtttcttccc ggtatctcat accggttctc taaacctctc tacctgcccg
     2701 ctgaaggtgc gtcaccagtt caactcgaca tcacctgccc cagttcagtc cgctttgagc
     2761 cggatggacc cgtaaagatg gatagaacag aggcagggtt tttgataaca gcaaatctgg
     2821 tgcccgcgat ccgggggaaa ctctggtatc ctgcaggcga tggcagcgcc ttcgcgggaa
     2881 tactatctat ctcagtacca catgcagcct ggcgatttga ggatgaggcg acagggacaa
     2941 tttacccgct caggcgctct gttccggtga tccctgatat tgactatcgg tcactgggta
     3001 ctgaaccagg tcttcatgta ttcttacctg aatctttcag tggtacagga acgatatcgg
     3061 tgctgcctca aaaccagact gtcacctgcc gggtcgtcaa ggggcagggt tatttctccc
     3121 tggcccgatt caacgacacg ctcagagcga ccgatgcaaa atgcatgcat tttgagtttt
     3181 cgatggaaga accgcgtgat ggaggcatac ggtttcctct cttttccctt cagcgctggt
     3241 atatccacct tcccaatccc cctcgcgtct caatcgaccc atcgggaaac cgtagcatag
     3301 agatcgagtg ggaagagtat ggaattgccc cgaaacgggt cttacttctc tggagaaaga
     3361 agaagtccgg cggtacatcc cggatatacg ttgaagagat cccgcctgca gtgcggaaac
     3421 ttacaattca gggagatcac gacctgccac tgtctcccgg catctattac ctgcagttct
     3481 acaggattct caatgattgg gacctcccgc cggcatcttt tggagagaga gcacccaacg
     3541 tcttcccgct ccgtatcgag cagagtaagg atcgcatatc agacgaacaa cctcctgaat
     3601 caggggggga tgtgccggga agttccgaac tcccgaaact cgatgccggg atcgagcggt
     3661 taatcggtga gatagagtcc ggcgatccgg agagactcac ccgggcgtta cgtgatctca
     3721 aggaagatta ctcgatacgc cgcataaacc atcacctgct ggagaagtat cccgcacagt
     3781 acgagcgact tcaggcagca ctttttgccg tgataatgga tgcccggata ccccaggagg
     3841 tcaggtccac ctctgttgtg ttgctcggaa aaagtttcaa ccaggagagg gaacatacgc
     3901 tcattacggt gctgggcagc cctgactctg caaaggatct gcgtattgcg atcctgaaag
     3961 ctctgggtca tggaaacacg gatgaatgcc tgaatgcact gttaaccaca cgtgatgatc
     4021 ctgatcccga tgtccgggtc gaattggcag catcgctggg gaacatcgat aacaaaggcg
     4081 ctgttaagcc attgatcgcg ctactggatg atgaatctgt ggtagtccag cgcaaggctg
     4141 cagcctcgtt aggctatacc cactctcatg cttcgtttga accccttatg tcgattgctc
     4201 tcgatgagaa gcgagatatg gtcctccgga ttacagcaat ttccgcactt gcgaaattcc
     4261 gtttaaatga gcaaactacc cgggaactgt tcaccttatc gaagaatgca ggcgaggatg
     4321 aagaggttcg taagatcgcc acgagtgtcc tgagcattca gggcagactg aatgttgagg
     4381 agaagaagag gcacatcgct gatctcatcc gggatgtgaa gacatcatca cacggaaagc
     4441 ggctctctgc cataaagcag ctgagtgcct ttggcgatga ggaagttatt cttgccctta
     4501 tggaagccgc ccaggatgag tctgtcgatg taaggagggt agctgcagac tcactccgcc
     4561 gaattaataa gccgatggct attccaactc tcatagaatt atgcgatgac ccggatgatg
     4621 atataaggga gactgccgaa tttgcctgta gacagctcct ggaagtgctc aagtcggatt
     4681 taaagcagac aagcgataaa cgagcaacag cagccgggca tctctccaga ataggggcac
     4741 ttgcagtaaa tcccctgctc gtcatgttac ggaaatctga gagtattcgg tcagaacgcg
     4801 cagattcagg tgagatccag aacagcatcg tggatgcttt atcaagaatg aatgatcccg
     4861 atgcaatcgc cctcatgatc cgggcgctac aggataagaa cgacctggtt gtgtggggtg
     4921 cagccaaagc tctcggtgct atcggcgatg cgagcgcgat cggcccactt aaggaggcac
     4981 tggcacggtg gtacaatcca atgagatgta tgacgccaac gacatacgta tggatggaga
     5041 tcgagaagac cctccaacgc ctgaatggtt gaatctgcag ggctgataac atgtatctct
     5101 cacctccgaa tatgggcagg tcatctgacc atgcggggca aaagaaaaag gcaagcctga
     5161 taaaagagtg cgccaaaggg gtatcatcga tagcaggact gctctatgtc actgaatccc
     5221 cttacaacaa cgcagtcgat cgctcagcgc tatatgcggt atctcaagac gacgtttcag
     5281 ataaacgatg ccgatctcgc cgccctgttc agggctacta tcgacgagca cgtatttgtc
     5341 aaagggaatt ccggacatct tcattttgaa tgcctggatg taggtgacaa tcattgcgtt
     5401 tttgctcctg agcttcgcgc gggtcgatgt ttcgtgcacg gtgtaatagg gatcggcgta
     5461 aaacttcctg ctccagctgt agtgcgaggg ataggccatg gggcatatga tgtcggcaac
     5521 cttgtccag
//
LOCUS       DAOQVJ010000039         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16456_length_5463_cov_3.647004, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000039 DAOQVJ010000000
VERSION     DAOQVJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16456_length_5463_cov_3.647004"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="pyrH"
                     /locus_tag="PKL26_01470"
     CDS             complement(<1..573)
                     /gene="pyrH"
                     /locus_tag="PKL26_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNS82394.1"
                     /translation="MTRVVLSLGGSILFPTLEAHTLRAWAPVLARLAQSVDLFVVVGG
                     GGEARRYIRAARDTWADEAFCDEIGILVTRLNATLLIGALGTAAYPRVALTPGEAMEY
                     AGKGKIVVMGGVTPAQTTDAVAAVLAERARAALLVNLTSVDGIYSADPKEHTDAHRYS
                     SMTPKELVEIVGCGGLAAGSNTVVDMVAAKV"
     gene            619..780
                     /locus_tag="PKL26_01475"
     CDS             619..780
                     /locus_tag="PKL26_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82395.1"
                     /translation="MLRCPSCGSRDVYCVVGGYIGQIYRCKSCGYRGSLIMECERPKN
                     GEEGEDPHL"
     gene            829..1842
                     /gene="amrS"
                     /locus_tag="PKL26_01480"
     CDS             829..1842
                     /gene="amrS"
                     /locus_tag="PKL26_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNS82396.1"
                     /translation="MHRALQWSPGEDGAVQCSLCSHRCTIREEKTGICGVRRNQGGVL
                     YATTYGKVSAEAVDPIEKKPLYHFLPGTLSYSLGSIGCNFRCEHCQNWHISRAERDAS
                     HLPDLSPGEGVRRAKAAGCASISWTYNEPTIWHEYALDMGTLARKEGLFTVYVTNGYI
                     TEEALRELSSMLTAYRVDIKSFSEEFYKKVCGARLQPVLDSAVVARELGMHVEVVTLV
                     IPGLNDSQEETGALIRWIIEHLGENTPVHFTRFHPDYHMQDRNSTPVSVLERIHRQAR
                     DLGLRYPYLGNVYPHPLESTYCPRCGALCIERRGFSAVFRNLEGNMCTRCGERIEFVG
                     NPG"
     gene            1823..2326
                     /locus_tag="PKL26_01485"
     CDS             1823..2326
                     /locus_tag="PKL26_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNS82397.1"
                     /translation="MSGTPDDPTRRTRFLADRMLGTLCRYLRFMGYDTESASGYREGN
                     TREDTLLLKRAQGEGRVLLTMDRELARRGGEGALLLEDRDVMEQLRVLTRTGLIVPAL
                     RLNRCSLCNTLLRAAREDEVSRARHAPRSTPGMEFFWCPRCRKLYWQGSHGKDLEKRI
                     KENILKD"
     gene            complement(2323..2718)
                     /locus_tag="PKL26_01490"
     CDS             complement(2323..2718)
                     /locus_tag="PKL26_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82398.1"
                     /translation="MHDFVRRELKRLYPSVDGWQIKNVPKSGADSGFIISRRLLGRFE
                     GSHVLVSFDRSVTSGTVNALKEMAASNPVSGVSSPRLILMAPQGAEVAGVPPEVQVLP
                     MQSFGYEGKELVWLKRRAQVSEKVAAKSA"
     gene            2911..5058
                     /gene="ppk1"
                     /locus_tag="PKL26_01495"
     CDS             2911..5058
                     /gene="ppk1"
                     /locus_tag="PKL26_01495"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNS82399.1"
                     /translation="MSGPSPVKGTAARGRERCDLLEDPGLFINRELSWIEFNRRVLRE
                     AAFQYHPLLERVKFLSIFANNLDEFFMIRVSGLHRQVEQGVLESPPDGMSPSRQLQAI
                     HQALVPLIGEQMALWKEDLLPALASFGIFVYRYGELPKEDREYLGEYFRHEIFPVLTP
                     LAFDRSHPFPFISNLSISLAVQVDDPRTGEDRFARVKVPTHLFPRLVRIPGRDRDSSL
                     REEARFVFLEDLISANLGLLFPGLDVVAAYPFRVTRDADMEIEEDEASDLLTAVEEGV
                     EMRRTGSPVRIEVVRGIPRKTCAILSRKLGVPQHMFYSQEAPLGMADLMQLCSLDRPD
                     LKDPPFAPSVPPALEREHEIFRSIRRKDVLLFHPYESFSPVIAFLRQAARDPQVLAIK
                     MTLYRVGAQSPVVKALLEARERGKAVAALIELKARFDEENNIGWARALEHAGVHVVYG
                     VVGLKVHAKVCMVIRKEKDGIVRYVHLGTGNYNASSSRIYTDIGVFSADPVLGEDVSD
                     LFNSLTGYASIPSYRTLLVAPQSIRSGILSRIGREIARHQEHGDGCIIFKMNALVDRE
                     CIMALYRASRAGVRVILQVRGICCLRPGIPGTSDNIAVTSIVGRFLEHARIYYFRNGG
                     QEEILLGSADLMPRNLDRRVEVLFPVKDPELHRAIADVILPVHLRDNEKTMELLVDGN
                     YAHMPGKEGEEPCNAQKWLIAHRGCWQHAKGIR"
     gene            5039..>5463
                     /locus_tag="PKL26_01500"
     CDS             5039..>5463
                     /locus_tag="PKL26_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNS82400.1"
                     /translation="MPKGSGDGRAQVCRYGAWFLSRVALEFREEIEVVKEGRDVEAVH
                     RMRVTSRRLRAGLPLFSQCFPGKAYKKISGGIRRITRDLGDARDLDVQIGYLEALIEE
                     HRDDGGPAVDGCTILLSMKRTRREALQPKVVADCTRLEQ"
BASE COUNT         1113 a         1614 c         1702 g         1034 t
ORIGIN      
        1 cacttttgcg gctaccatgt ccaccaccgt attcgatccg gctgcaaggc caccgcaccc
       61 cacgatctcc accagctcct ttggtgtcat tgaggagtaa cggtgtgcat ccgtgtgttc
      121 tttcgggtcg gcggaataga tgccgtccac cgaagtgagg ttgacaagga gggcagccct
      181 cgcccgctcc gcgaggaccg cggcgaccgc atcggttgtc tgtgcgggtg tgacccctcc
      241 catcacgacg atctttcctt ttcctgcgta ttccatggcc tcgccgggag tgagcgccac
      301 gcggggatag gccgcggtcc cgagtgcacc gataaggagg gttgcgttca gtcgggtgac
      361 caggatccca atctcgtcac agaaggcctc gtctgcccag gtgtccctgg ccgccctgat
      421 ataccgccgg gcttccccgc ccccccccac gaccacgaag aggtctaccg actgtgcgag
      481 cctcgccagc acgggtgccc aggcgcggag ggtatgcgcc tcgagggtgg gaaagagaat
      541 tgagcctccg agggagagga ccacgcgtgt catgctacac tatcatttct ccatgcggca
      601 catataagtg ttgaagatat gctgcgatgt ccgtcctgcg ggagcaggga tgtctattgc
      661 gtggtcgggg gatacatcgg ccagatctac cggtgcaagt cctgcgggta ccgtgggtct
      721 ctgattatgg agtgcgagcg ccctaaaaat ggggaagaag gggaggaccc gcacttgtag
      781 gagacccggt gcattacctt aataattcct gcacgcctga ttgagaacat gcacagggcg
      841 ctccagtggt caccgggaga ggatggtgcg gtgcagtgct cgctctgcag ccaccgctgc
      901 accatcaggg aggagaagac cgggatctgc ggtgtcagga ggaaccaggg tggggtcctg
      961 tatgccacga cctatgggaa agtgagcgcg gaagcggtcg atcccatcga aaagaagccg
     1021 ctttaccact tccttcccgg caccctctcg tactcccttg ggagcattgg ctgcaatttc
     1081 cgttgcgagc actgccagaa ctggcacata tcccgcgcag agcgggacgc atcgcacctc
     1141 ccggatctct cacccgggga aggggtccgc agggcgaagg cggccgggtg cgccagtatc
     1201 tcatggacct acaacgagcc cactatctgg cacgaatatg cactcgacat ggggactctt
     1261 gcaagaaaag aagggctttt caccgtgtac gtgaccaacg ggtacatcac cgaggaggcg
     1321 ctccgtgaac tctcctccat gctgacagcc tatcgcgtgg acatcaagtc gttctcggag
     1381 gagttctaca agaaagtatg cggagcccgc ctccagccgg tgctcgactc tgcggtcgtg
     1441 gcgagggaac tcgggatgca cgtcgaggtc gtcaccctcg tcatcccggg gttgaacgac
     1501 agccaggaag agacgggcgc cctcatcagg tggatcatcg agcaccttgg tgagaatacc
     1561 cctgttcact tcacccgctt ccaccccgat taccacatgc aggacaggaa ttccacgcct
     1621 gtttcggtcc ttgaacggat ccaccggcag gcaagggatc tcgggctccg gtatccctac
     1681 cttggaaatg tctatcccca ccctctcgag agcacctact gcccccggtg cggggcgctc
     1741 tgcatcgagc gcaggggatt ttccgcagtc ttccggaacc tcgaggggaa tatgtgcaca
     1801 aggtgcggtg aacgcattga gtttgtcggg aaccccggat gatccaacgc ggaggacccg
     1861 gtttctcgcg gaccggatgc tcggcaccct ctgccggtac ctgcgcttca tgggctatga
     1921 cactgagagc gcatcggggt accgcgaagg aaatacccgc gaggacacat tgctcctgaa
     1981 gagggcccaa ggagaaggga gggtgctgct cacgatggac cgcgagcttg cacgcagggg
     2041 tggagagggg gcactcctcc tcgaagaccg ggatgtcatg gagcagttgc gggtgctcac
     2101 gaggaccggg ttgatcgtgc ctgcactcag gctgaaccgg tgctcgctct gcaataccct
     2161 ccttcgtgct gccagggaag atgaggtttc gagggcccgc cacgccccac gaagcacacc
     2221 gggcatggag ttcttctggt gcccgcggtg caggaagctc tactggcagg gctctcacgg
     2281 aaaagacctg gaaaaaagaa taaaagagaa tatattgaag gattaagcgg attttgctgc
     2341 gaccttctcg cttacctggg ccctgcgctt cagccagacc agttccttcc cctcgtagcc
     2401 gaaactctgc atagggagga cctggacctc gggcggaacg cctgcgacct ccgcaccctg
     2461 cggggccatc aggatcaggc gcggcgaaga aacccccgac acagggttcg atgcagccat
     2521 ttccttcagc gcgttgacgg tcccggaggt cacgctcctg tcgaagctga ccaggacatg
     2581 cgaaccttcg aagcgaccga gcagcctccg ggatatgatg aaccctgagt cggcaccgga
     2641 cttcgggacg tttttgatct gccagccatc cacgcttggg taaagcctct tgagctctct
     2701 tcgtacaaaa tcatgcatga tgtcttcatt ggatcccatg gcgaatcaca gcaagtgtta
     2761 gttgtgcact ggtatggtaa aacagttttg aaatgggtat gaagatctcc aaatcccccg
     2821 gataatcccc ctggaattgc ctcctttcgg cagcatgctt atgtacccgg cattccagag
     2881 tgatagagta gccttgatag gtagaagaac atgagcgggc cttctccagt gaaagggaca
     2941 gcagcccggg gcagggagcg ttgcgatctc cttgaagacc cgggactctt catcaacagg
     3001 gaactctcct ggatcgagtt caacaggaga gtgctcaggg aggcggcgtt ccagtaccat
     3061 ccgctcctgg agcgggtcaa gttcctctcg atatttgcca ataacctcga cgagttcttc
     3121 atgatccggg tctccgggct gcaccgccag gtggagcagg gagtcctcga atctccaccg
     3181 gacgggatga gcccgtcccg gcagctccag gccatccacc aggcccttgt cccgctcatc
     3241 ggggagcaga tggcgttatg gaaggaggat ctcctccctg ccctggcatc gtttggaatt
     3301 tttgtatacc ggtacgggga gctgccgaag gaggacaggg aatacctcgg ggagtacttc
     3361 aggcatgaaa tcttcccggt gctcaccccg ctggcattcg accgctccca cccgttcccc
     3421 ttcatctcca acctctccat atccctcgcg gtgcaggtgg acgatccgag gaccggggag
     3481 gatcggttcg caagagtgaa ggtccccacc cacctcttcc cccgcctggt ccgcatcccc
     3541 ggacgcgacc gggacagcag tcttcgcgag gaggcccggt tcgtcttcct cgaggatctt
     3601 atcagcgcca acctgggcct cctcttcccc gggctcgatg tagtagccgc gtacccgttc
     3661 agggtgacac gcgatgcgga catggagatc gaggaggacg aagcatcgga cctgctgacg
     3721 gcggtcgagg aaggggtgga gatgcgccgg acaggctcgc cggtacgaat cgaggtcgtc
     3781 aggggcatcc cccgaaagac ctgcgcgatc ctcagccgta aactgggggt gccgcagcac
     3841 atgttctatt cccaggaggc tccgctcggc atggccgacc tgatgcagct ctgctccctg
     3901 gaccggcccg acctgaagga cccgccgttc gccccatccg ttccgcctgc gctcgaaagg
     3961 gagcacgaga tctttcgcag catcaggaga aaggacgtcc tgctctttca tccctacgag
     4021 agtttctccc ctgtgatagc gtttctacgc caggccgcgc gcgaccccca ggtgctggcc
     4081 atcaagatga cgctctaccg ggtgggcgca cagtccccgg tggtaaaagc gctcctggag
     4141 gcacgggagc ggggcaaggc agtggccgcg ctgatcgaac tcaaggcgag gttcgacgag
     4201 gagaacaata tcgggtgggc gagggcgctc gagcacgcgg gggttcacgt ggtgtacggt
     4261 gtcgtgggtc tcaaggtgca tgcaaaggta tgcatggtaa tcaggaagga gaaagacggc
     4321 atcgtcaggt acgtgcacct cgggaccggc aattacaatg cctccagcag ccgcatctac
     4381 acagacatcg gagtcttctc ggcagatcct gtcctcgggg aggatgtatc ggacctgttc
     4441 aatagtctca cagggtacgc cagcatccct tcgtaccgga cactcctcgt agccccgcaa
     4501 tccatcagga gcgggatcct gtcccggatc gggcgggaga tcgcgcggca ccaggagcac
     4561 ggggacgggt gcattatctt caagatgaac gcgctcgttg atcgcgaatg catcatggcc
     4621 ctgtaccggg cctcccgcgc gggtgtcagg gtcatcctcc aggtacgcgg tatctgctgc
     4681 cttcgcccgg ggatacccgg cacgagcgac aatatcgccg tcacgagcat cgtggggcgt
     4741 ttcctcgagc atgcacgcat atattatttc cgtaacgggg gacaggagga gatcctactc
     4801 gggagcgcag acctgatgcc gaggaacctc gacaggcggg tagaggtgct ctttcccgtg
     4861 aaggacccgg aacttcaccg ggcgatcgcg gatgtcatcc tccctgtgca cctgcgggac
     4921 aacgaaaaga ccatggagct gctcgtggac ggcaactatg cccatatgcc gggaaaggag
     4981 ggagaagaac cctgcaatgc ccagaaatgg ctcattgcac accgggggtg ctggcagcat
     5041 gccaaaggga tcaggtgatg gcagagcgca ggtctgccgt tacggggcat ggttcctctc
     5101 ccgggtcgcc ctcgaattcc gcgaagagat cgaagtggtg aaagaaggga gggatgtcga
     5161 ggcagtccac cggatgaggg tgacatcccg gaggttgcgg gcgggcctcc cgctcttctc
     5221 gcagtgcttc cccgggaagg cctacaagaa gatctctggg ggtatccggc gcatcacgag
     5281 ggatctcggg gatgcccgtg accttgacgt gcagatcggg tacctcgagg cactgatcga
     5341 ggaacacagg gatgatggcg gaccggcggt ggatgggtgc acgatactcc tctcgatgaa
     5401 gaggacaagg agggaggcat tacagccgaa ggtcgttgcg gattgcacga ggctcgagca
     5461 gaa
//
LOCUS       DAOQVJ010000040         5418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16625_length_5418_cov_4.312325, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000040 DAOQVJ010000000
VERSION     DAOQVJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16625_length_5418_cov_4.312325"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKL26_01505"
     CDS             complement(<1..405)
                     /locus_tag="PKL26_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82401.1"
                     /translation="MTVLQSLKNFVRSKSIHVAYVDVGSCNGCDIEVLACLAPRYDIE
                     QYGIFVHNNPREADVLLVIGAYTPGWEGKLEGLWEKIPDPKVAIAIGNCPISGCLFNR
                     PHTLIDPPVAKHIPIAAQVPGCPPRPTEILSAI"
     gene            complement(402..791)
                     /locus_tag="PKL26_01510"
     CDS             complement(402..791)
                     /locus_tag="PKL26_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1959 domain-containing protein"
                     /protein_id="HNS82402.1"
                     /translation="MREPIYEKDLIAMKYAILESRRHDRMVREIAAEFGIPQNRMRRY
                     LMDCCDMLLLENLPARYEQGKRVQEEAPEPERQLGAHLFTRAVPLLGEDRMLQILDRV
                     KELARGGTPIDQAVRVGKEMIREAITG"
     gene            complement(788..1030)
                     /locus_tag="PKL26_01515"
     CDS             complement(788..1030)
                     /locus_tag="PKL26_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82403.1"
                     /translation="MIREVLVVGTLIYLLIIAMQCFDRPALWLQGLAIGAALALATTV
                     YWCREDLRLRTWEAIALWAAVLLFVAYGLARLGGLI"
     gene            complement(1027..1869)
                     /locus_tag="PKL26_01520"
     CDS             complement(1027..1869)
                     /locus_tag="PKL26_01520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNS82404.1"
                     /translation="MIAELLFAVFFGLLLLGIHRKVIARIQRRPGPPVWQEILHMLKF
                     SFKTTWVPATASTALFCGVVLVAIGIWTAALVVVLSGGSLLVIFAIYMLHKIVEHGFG
                     LSSGSPYGKFGGVRSVISAASEIPLFVSVAAIAIFTQSLMITDIIAYQEINGPLLITV
                     PLAALAMYVVLLSKMPFGPFSIVESKELVSGYKTEHFGVWRAGLEVCNGLKTYVLLLT
                     FILVFIGNLPFWLVILAMVIILITLSFVCALSPMLSPYDSVTVQTLIAGLMVAYVALL
                     GVMS"
     gene            complement(1872..2075)
                     /locus_tag="PKL26_01525"
     CDS             complement(1872..2075)
                     /locus_tag="PKL26_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82405.1"
                     /translation="MNKQYALALMLVSAVSAAGALALNYWGILPFWAYAAVLIAGFPL
                     FLLGLGLYWMAREGEADMPFLGY"
     gene            complement(2086..>2261)
                     /locus_tag="PKL26_01530"
     CDS             complement(2086..>2261)
                     /locus_tag="PKL26_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82406.1"
                     /translation="PVAAKALLVLGVMGEGMAPFYAAKAEMFRAPGAPYVIMCSLSSL
                     LIFLRVAEVILLL"
     assembly_gap    2262..2361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2362..2609)
                     /locus_tag="PKL26_01535"
     CDS             complement(<2362..2609)
                     /locus_tag="PKL26_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82407.1"
                     /translation="MNEIGGAPLFFLEFGDIVIYFTPFTLTLFILALVFTVFVLVSRP
                     ERQIDIAFGGDAYYAKEISLSELRFRRFMSIACGLACMG"
     gene            complement(2606..3208)
                     /locus_tag="PKL26_01540"
     CDS             complement(2606..3208)
                     /locus_tag="PKL26_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2105 family protein"
                     /protein_id="HNS82408.1"
                     /translation="MWNEYSIGLAAAFACALIGFLALVWERDDLHKLLLIDLVETASL
                     AVIALIGTDLSEALILPGLVVGIAELLALSQIYLTKEGYQHHPVTRLNIEVMMRSPAP
                     VIISAILVVYGIILSGFTGGGIAGLGIVFLLMSRSFSGDFHALEMASGISWALWVAAF
                     FVFMFAPQWWYMALMGAAIGILLKVIAKMSLVGAMWGEPE"
     gene            complement(3215..3664)
                     /locus_tag="PKL26_01545"
     CDS             complement(3215..3664)
                     /locus_tag="PKL26_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2106 family protein"
                     /protein_id="HNS82409.1"
                     /translation="MIIQRISRVLSNFDNILPVFAAACAAMSVFVLLSLPLPYSEDQL
                     YPKQIVPGSTLNPYDRGGPPFTQVSIHSQYPENSPTAGYVTAYLTPLSLFISEHTAYM
                     GTTIVAHPGGILDEILYNTRGLDTVVEPTILFVAFAIAAYIFRKREA"
     gene            complement(3661..3900)
                     /locus_tag="PKL26_01550"
     CDS             complement(3661..3900)
                     /locus_tag="PKL26_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2107 family protein"
                     /protein_id="HNS82410.1"
                     /translation="MNPEFILGLVLLLGGTIAAAFPVPKTYLNRLINLEISAWGLLLL
                     MLSYNEALALLTFGGVSALSVYIFVRVIQKKEAPA"
     gene            complement(3897..4127)
                     /locus_tag="PKL26_01555"
     CDS             complement(3897..4127)
                     /locus_tag="PKL26_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2108 domain-containing protein"
                     /protein_id="HNS82411.1"
                     /translation="MNNLAVMAFGALAIIGALLTIVERDPFRKLISLAILVGGSVPFI
                     VDRGLLDVAIAVALIAPLSTIFILMVCGRGEQ"
     gene            complement(4120..4377)
                     /locus_tag="PKL26_01560"
     CDS             complement(4120..4377)
                     /locus_tag="PKL26_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 domain-containing protein"
                     /protein_id="HNS82412.1"
                     /translation="MTPQEAALAVCAVIAVYAAIRVALEPDTLRKLPFLNVLSFAVAG
                     SIALLIPHPLSIVAAAAYFVGSTLESNAIASTYAGGLDRRE"
     gene            complement(4374..4871)
                     /locus_tag="PKL26_01565"
     CDS             complement(4374..4871)
                     /locus_tag="PKL26_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82413.1"
                     /translation="MDSILPAFARILVAAAVCWLNFILIDLFFRLPAGGGVVGATAIG
                     THIERGGGAIHGGYMMGNIVSSPDASAGTLLASCGVYIAGIPGGLLATFLVFIGNRIC
                     HDRGYAGTTGAITATFIVWGFSQIGFSPAEFIAGMVIAILTIQGISHRHSSRLLAYLW
                     KVREG"
     gene            complement(5090..>5418)
                     /locus_tag="PKL26_01570"
     CDS             complement(5090..>5418)
                     /locus_tag="PKL26_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNS82414.1"
                     /translation="KAIPRQISEVELSKNMLIIVLYVLIIFVATIISLHIAPTDFSVH
                     QVVFDEVSAMSNVGIGVGYMTPGSPIPIKWIFILLMWVGRLEIIPVLILIMGLVRGFE
                     ARVAGK"
BASE COUNT         1208 a         1525 c         1617 g          968 t
ORIGIN      
        1 tattgccgag aggatctcgg ttggcctcgg cgggcagccg gggacctgtg cggcgatcgg
       61 gatgtgcttt gcgaccggcg ggtcgatgag ggtgtgcggg cggttgaaga ggcaccccga
      121 tatggggcag ttcccgatgg caattgccac cttcgggtcc gggatcttct cccagagccc
      181 ctcgagcttc ccctcccacc cgggcgtgta ggcgccgatg accaggagga catcggcctc
      241 ccttgggttg ttgtggacga agataccata ctgctcgata tcataccgcg gtgccaggca
      301 tgccaggacc tcgatgtcgc acccgttgca ggagccgaca tccacgtacg cgacgtggat
      361 ggacttcgac ctgacaaagt ttttcaggga ctgcagaacc gtcatccggt gatcgcctcc
      421 ctgatcatct cctttcccac ccgcaccgcc tggtcgatcg gcgtcccccc gcgggccagt
      481 tccttaaccc tgtcgagtat ctgaagcatc ctgtcctctc caaggagggg gacagcacgc
      541 gtaaagaggt gtgctccaag ctgccgctcg ggttcgggag cctcctcctg aaccctcttc
      601 ccctgctcgt agcgtgccgg gaggttctcg aggaggagca tgtcgcagca gtccatgagg
      661 tagcgccgca tcctgttctg ggggatgccg aattcggcag caatctcgcg caccatccgg
      721 tcatggcgcc ggctttcgag gattgcatac ttcatggcga tgaggtcttt ctcgtagatc
      781 ggttccctca tatcaggcct cccagcctcg caaggccata tgcaacaaag aggaggactg
      841 ctgcccacaa tgcaatcgcc tcccatgtcc ggagacgcag atcctcccgg caccagtaga
      901 cggtggttgc gagggcaagc gctgccccaa tggccaggcc ctgcaaccat agagcaggac
      961 ggtcgaaaca ctgcatggcg attatcagga gatagatgag ggtgccaacc accaatactt
     1021 ctctgatcat gacatcaccc cgaggagggc aacatatgcg accatcaggc cggcaatcag
     1081 tgtctggaca gtcacactgt cgtatggtga gagcatggga gagagggcgc acacgaacga
     1141 gagtgtgatg agaatgataa ccatcgcgag aatgaccagc cagaacggga gattcccgat
     1201 aaacacaagg atgaaggtca ggaggaggac ataggtcttc aggccgttgc agacctcgag
     1261 gcccgccctc cacacgccga agtgctccgt cttgtatccg ctgacaagct ccttgctctc
     1321 cacgatcgag aatgggccaa acggcatctt ggagaggagg accacgtaca tcgccagcgc
     1381 cgcgagcggg acggtgatga gaagcggacc attgatctcc tggtacgcga tgatatccgt
     1441 gatcatcagc gactgggtga agattgcgat cgctgcgacg ctgacaaaga gggggatctc
     1501 ggaagccgcc gagatgaccg atcggacccc tccgaacttt ccatacgggg acccggacga
     1561 gagcccgaac ccatgctcga cgatcttatg gagcatgtag atggcaaaga tgaccaggag
     1621 gcttcctccc gagagtacca cgacgagcgc agcagtccag atcccgatgg cgaccaggac
     1681 tacgccgcag aagagcgcgg tgctcgcagt ggccggaacc cacgtggtct tgaacgagaa
     1741 cttcagcata tgcaggatct cctgccacac cgggggcccg ggccgcctct ggatgcgggc
     1801 aatcaccttc cggtgaatgc caagcaggag gagcccgaag aacacggcaa agagcagctc
     1861 ggcgatcatt gctagtaccc caggaacggc atgtccgcct caccctcgcg ggccatccag
     1921 tacagcccga gccccagcag gaaaagcggg aacccggcaa tcaggactgc tgcataggcc
     1981 cagaagggga gaatgcccca gtaattgaga gccagtgctc ctgctgccga aaccgccgaa
     2041 accagcatca gggcaagggc atactgcttg ttcattcgat tcacctcaca ggagcaggat
     2101 cacctcggcc actctcagga agatcaggag tgacgagagc gagcacatga tcacataggg
     2161 ggcaccgggt gccctgaaca tctcggcctt ggccgcgtag aatggggcca tcccctcccc
     2221 catcaccccg agcacgagaa gtgcctttgc ggcaaccggg annnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn ncccatgcac gcaagaccgc aggcgatcga catgaagcgg
     2401 cggaaccgga gttcggagag agagatctcc tttgcatagt acgcatcccc gccgaatgcg
     2461 atgtcgatct ggcgctcggg cctgctcacc agcacaaaca ccgtgaatac gagtgccagg
     2521 atgaagagag tgagagtgaa gggtgtgaag tagatcacga tgtccccgaa ctccaggaag
     2581 aagaggggtg ctcccccgat ctcgttcatt cgggttcacc ccacatcgcc cctacgaggg
     2641 acatcttggc aatcaccttc aggaggatcc cgattgcagc gcccatgagg gccatgtacc
     2701 accactgggg ggcgaacatg aacacgaaga acgccgcgac ccagagcgcc caggagatcc
     2761 cgctcgccat ctccagggca tggaagtccc cggagaagct cctgctcatc aggaggaaca
     2821 cgatgccgag gcccgcgatc cccccgccgg tgaaccctga gaggatgatg ccgtacacga
     2881 caaggatggc ggagatgatg accggggccg gggaccgcat catcacctcg atgttcaggc
     2941 gcgtgacagg gtggtgctgg tacccttcct ttgtcaggta tatttgcgag agtgccagaa
     3001 gctcggctat tcccaccacc aggccgggaa gaatcagcgc ctctgataga tcggtcccga
     3061 tcagcgcgat caccgcaagc gacgcggtct cgacgaggtc gatgagcagg agtttatgga
     3121 ggtcgtcccg ttcccagacg agagccagga acccgatgag cgcacatgca aatgcggcgg
     3181 caagcccgat ggagtattcg ttccacatct ttttttaggc ctccctcttc ctgaagatgt
     3241 atgccgcgat tgcaaatgca acaaacagga tggtcggttc aacgactgta tcaagacccc
     3301 tcgtattata gaggatctca tcgaggatgc ctcccgggtg tgcgacgata gtggtcccca
     3361 tgtaggcggt atgctccgag atgaagagag agaggggtgt aaggtatgcc gtgacatacc
     3421 ctgcagtggg ggagttctcc gggtactggg aatggattga aacctgggtg aagggcggcc
     3481 cgcccctgtc ataagggttg agcgtgctcc ccgggacgat ctgctttgga tacagctggt
     3541 cttcagaata tggcagcggt agcgagagga ggacgaagac cgacatcgcc gcgcaggctg
     3601 cggcgaatac cggcaggata ttgtcgaagt ttgagagaac cctcgatatc ctctggatga
     3661 tcacgccggc gcctcctttt tctggattac tctcacgaag atatataccg agagcgcaga
     3721 aaccccccca aatgtcagaa gcgcaagcgc ctcattgtag gagagcatca gcaggagaag
     3781 tccccatgcc gagatctcca ggttgatcag ccggttcagg taggttttag gtaccgggaa
     3841 tgctgcagcg atggttccac ccagcaggag cacaagaccg aggatgaact ccgggttcat
     3901 tgctctcccc tcccgcagac catgaggatg aagatggtgg agagcggggc gatgagcgcg
     3961 accgcgatgg caacgtcaag gagtcccctg tcaacaatga acgggaccga tcccccgaca
     4021 aggatggcga gggagatcag tttccggaag ggatcacgct cgacaatggt gagcagggcc
     4081 ccgatgatgg ccagcgcccc gaacgccatc actgcgaggt tattcacggc gatccagccc
     4141 tcctgcgtag gtgctggcga tggcgttcga ctcgagtgtc gacccgacga aataggccgc
     4201 ggcggcgacg atgctcagcg ggtggggtat gaggagggca atactccccg caacggcaaa
     4261 agagagcaca ttcaggaagg ggagtttcct gagtgtgtca ggctcgagcg caaccctgat
     4321 ggctgcgtac actgcgatga ctgcgcacac cgcgagggcg gcttcctgcg gcgtcatcct
     4381 tccctcacct tccagaggta ggcaaggagg cggctggaat ggcggtgtga tattccctgg
     4441 atcgtcagga tggcgatcac catccctgcg atgaactcgg caggggagaa cccgatctgg
     4501 gaaaagcccc agacgatgaa cgttgcggtg atggcgccgg tagtgcctgc atacccgcgg
     4561 tcatggcata tacggttgcc gatgaagacc aggaatgttg cgagcagccc tcctggtatc
     4621 cctgcaatat agaccccgca ggaggcaagg agtgtccccg ccgaggcgtc gggggaagag
     4681 acgatgttgc ccatcatgta gccgccgtgg atggcgcctc cacccctttc gatgtgtgtg
     4741 ccgatggcgg tcgcccccac cacgcctccg cccgcaggga gcctgaagaa tagatctatc
     4801 aatatgaaat tgagccagca gacggctgct gcaaccagga tacgggcgaa tgccggaaga
     4861 atgctatcca tcgggccgtc aacctacagg tggttaggct gtgagaagaa gaatattttg
     4921 attttggtaa attgcgccga atggggtgtg ataaggttcc tgattggcac gcggaggtgg
     4981 aaagatcgag aatgtgcctg cattagtcct gggatattcc cttgtgtcct tctgtacaac
     5041 cggataatcc atattcccgc ttccgtggaa aagaaagcgt tactccctgc tacttccctg
     5101 ccactcgtgc ctcaaagccc cggacgagcc ccatgatcag gatgaggacc ggtatgatct
     5161 cgagcctccc gacccacatc aggaggataa aaatccactt gatcggaata gggcttcccg
     5221 gggtcatgta ccctacgccg attcccacgt tgctcattgc cgagacctcg tcaaacacga
     5281 cctggtgtac ggaaaagtcc gtcggagcga tatgcaggct gataatggtg gctacgaaga
     5341 tgatcaggac gtaaaggacg atgatcagca tgttcttgga gagctcaacc tcggagatct
     5401 gcctggggat ggcctttc
//
LOCUS       DAOQVJ010000041         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16627_length_5417_cov_3.996270, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000041 DAOQVJ010000000
VERSION     DAOQVJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16627_length_5417_cov_3.996270"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKL26_01575"
     CDS             complement(<1..558)
                     /locus_tag="PKL26_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNS82415.1"
                     /translation="MKSICGVEGVTAWGLKEGKFGLALIRAEGEAAGVFTSNLMKAPP
                     VSLMEERIRGGVLSAVMANSGCANAYTGARGYRDAAEMAGICAEVMQVAPEEVGVAST
                     GVIGRYLNLPLIRDQCKRVAPNLARSEEAEVRAARAIMTTDITEKHALVRCENFSVGG
                     VTKGSGMIAPNMGTMLAFIYTDAEVP"
     gene            complement(575..>707)
                     /locus_tag="PKL26_01580"
     CDS             complement(575..>707)
                     /locus_tag="PKL26_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82416.1"
                     /translation="EIEDAASVMLSKKIARLPVVRGETLVGIVARADIVRGVAAPGQ"
     assembly_gap    708..717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<718..1074)
                     /locus_tag="PKL26_01585"
     CDS             complement(<718..1074)
                     /locus_tag="PKL26_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82417.1"
                     /translation="MLIREVMTKNPVTVQGDAPVRDAAALLRAHKIGGLPVMDGEKLV
                     GMITESDILRLLETGKISDDLWLPSPLEVIEVPIREIINWEKTKHALASIGNIQVRKV
                     MSHPAITIGEDEDVEEA"
     gene            complement(1093..2079)
                     /gene="argC"
                     /locus_tag="PKL26_01590"
     CDS             complement(1093..2079)
                     /gene="argC"
                     /locus_tag="PKL26_01590"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNS82418.1"
                     /translation="MDIAIIGASGYAGGDLIRLLLGHSRARVSCVTSRKLAGKPVESI
                     HPQFKGLLDLEFSNPSPKDLEADVAFLAVPHTAAMAYARPLLSRGIKVIDLSADYRLP
                     KEIFEKVYGVPHTDYFTAPYGIPELRRDSYRDADFVSNPGCFPTGATLAAAPLARYAH
                     TVIYDSKTGVSGAGDNPSATTHYPNVGDNVNPYKWTSHRHLAEMKQELVSLGSHAKCY
                     FTPHLVPVNRGILTTAHILLKEPMGQEEVELLYRNYYEREFFVRLQNPILAAVRGTNF
                     CDIRVESEGDRVVAVSAIDNLGKGASGQAIQNMNIICGFDERDGLTGAGLLP"
     gene            2544..4445
                     /gene="acs"
                     /locus_tag="PKL26_01595"
     CDS             2544..4445
                     /gene="acs"
                     /locus_tag="PKL26_01595"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNS82419.1"
                     /translation="MAESFEVPLSGDEKTYIPDPSYKQNAWTKDYTTAYKEFMADPDG
                     FWEKQAHHFEWIRKWDRVLEWNYPYAKWFVNAKLNITANCLDRHVNNHRRNKVALIWR
                     GEEGEERTFTYKKLFGEVCRFANGLKKLGVAKGDRVCIYMPLVPEQVIAMLACARIGA
                     VHSVVFGGFGVQALNMRIKDAEAKIVITADVTYRRGKTIPLKTIVEEAVINAPSVEKV
                     VVLSREVEQPVEIHKEMEVDYFELMEGVPTTCEAEVMDAEDPLFILYTSGSTGTPKGI
                     VHTCAGYMVGTAYTTQHVFDVKDNDVYWCTADTGWITGHSYVVYGPLAIGATIVLAEA
                     VPDYPDAGAFWKIVEDLGVTIFYTAPTAIRMFMKVGEQWPNKYDLSSLRILGSVGEPL
                     NPEAFEWYYQVIGKKKCPIVDTWWQTETGMQMITTMVGEPMRPGFAGRPIPGVVADVV
                     DKKGKPVAPGNGGFLVIQRPWPAMLRTVYKNDERYRTYWNTIPDCYTAGDLAVKAKGD
                     EYIMIIGRADDIIIVAGHNIGTAEVESALVSHQSVAEAAVIGKPEPMKGNIIKAFVIL
                     RVGFSPSEKLKSDLIYHVRMTLGPIAMPSEIEFVDKLPKTRSGKIMRRVLKAQEMGID
                     PGDVSTLEE"
     gene            4479..>5417
                     /locus_tag="PKL26_01600"
     CDS             4479..>5417
                     /locus_tag="PKL26_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNS82420.1"
                     /translation="MPDDGSMFGMPAEKGRWVLVFLGMAINLCLGSIYSWSVFVNPLT
                     DYFTGSLGQSVTANEVLMPFSVFLAFFAIAMPLAGKYIETLGPRNVTIIGGVLTGLGW
                     LLASMVTSVQMLYLVYGVIGGVGVGIAYGVPVAVAARWFPDRRGLAVGLTVLGFGFSA
                     FLTANIAGYLISSFGVMDTFRIFGIAFVVLIILLALPLRFPAPGWKPRGWNTPVPKPG
                     EETCECDRGAMLRTPTFYGLWACFFIGCLAGLMAISIAKPVGTEIGIEAGLATLLVGL
                     FAIFNGGGRPVFGSLTDRITPRNTALASFVLIALASL"
BASE COUNT         1090 a         1600 c         1578 g         1139 t
ORIGIN      
        1 gggcacctcc gcgtcggtgt agatgaatgc gagcatcgtc cccatgttgg gggcaatcat
       61 cccagatccc tttgtgaccc cgccgaccga gaagttctcg cacctgacga gcgcgtgttt
      121 ctcggtgata tcagttgtca tgatggctcg ggcggcacgc acctcggctt cttcgctcct
      181 ggcaaggttc ggggcgaccc ttttgcactg gtcccgtatg agggggaggt tgaggtagcg
      241 cccgatcact ccggtactgg ccactcccac ctcctcagga gcgacctgca tcacctctgc
      301 acagatcccg gccatctccg cggcatcccg gtagcccctg gcccctgtgt atgcattcgc
      361 gcacccgctg tttgccatga ccgccgagag cacgcccccg cggatacgct cctccatcaa
      421 actaaccggc ggggccttca tgaggttcga agtaaatact cctgccgcct cgccttcagc
      481 ccttatcagg gcaagaccaa actttccctc cttcaagccc cacgctgtca ccccttccac
      541 gccgcagatg ctcttcattc agtgactccc tccattattg gcccggagcc gccactcccc
      601 ggacgatatc cgcccgggcc acgatcccga cgagggtctc acccctgacc acgggaagac
      661 gcgcgatctt cttcgagagc atcaccgacg cggcgtcctc gatctccnnn nnnnnnntgc
      721 ctcctcaacg tcctcatcct caccgatagt gattgcgggg tgactcatca ccttcctgac
      781 ctggatattc ccgatggatg caagagcgtg cttcgtcttc tcccagttga tgatctccct
      841 gatcggcacc tcgatgacct caagcgggga aggaagccac agatcgtctg agatcttccc
      901 tgtctcgagc agcctgagaa tgtcggactc cgtgatcatt cccaccagct tctcgccgtc
      961 cattacaggg agacccccaa tcttgtgtgc acggagcagc gcggctgcat cccggaccgg
     1021 ggcgtccccc tggaccgtga ccggattctt tgtcataacc tctcgtatca gcattctttt
     1081 cattcacctc gttcagggaa gcagccctgc acccgtgagg ccgtcacgtt cgtcaaagcc
     1141 gcagatgatg ttcatgttct ggattgcctg gccgctcgca cccttgccaa ggttgtcgat
     1201 ggccgatacc gccaccaccc ggtcaccctc gctctccacc ctgatatcgc agaagttggt
     1261 gcctctgact gctgcaagga tcgggttctg aagcctgaca aagaactccc tctcgtagta
     1321 gttccggtag agaagctcga cctcctcctg ccccatcggc tcttttagca ggatatgggc
     1381 ggtggtgagg attccccggt ttaccgggac aaggtgcgga gtgaagtagc actttgcatg
     1441 ggaaccgagg cttacgagct cctgcttcat ctctgcaagg tgccggtggg aggtccactt
     1501 gtaggggttc acgttgtccc ccacgttcgg gtagtgggtc gtggccgaag ggttgtcccc
     1561 ggctccagag actccggtct tggaatcgta tatcaccgtg tgggcatagc gtgcgagcgg
     1621 cgcggcggcg agggtggctc cagtggggaa gcatcccggg ttgctgacaa aatcggcatc
     1681 ccggtaagaa tccctgcgca gttccgggat accgtaggga gcggtgaaat aatcggtatg
     1741 cggcactcca tagaccttct caaagatctc ttttgggagc cggtagtcgg cagagaggtc
     1801 gataaccttg atcccgcggg agaggagggg ccttgcgtag gccatggccg cggtgtgggg
     1861 gaccgccagg aatgcaacat cggcttcaag gtcttttggg gaaggatttg aaaattccag
     1921 gtccagcagg cccttgaact ggggatggat ggattcgaca ggctttcccg ccagtttccg
     1981 ggatgtcacg caggagacgc gtgccctgga atggccgagc agcaggcgga tcaagtcgcc
     2041 gccggcgtag ccggaggccc cgatgatggc aatatccata catctcgttt cgatttgcag
     2101 atgttaatcc tttttccctt cattttcacc ggtatttgcc tcttgcgcga ccccgtgccc
     2161 cagattccgg gtcccgcgga aatgcccgga gtcaggaacc cggcccggca cggagccatg
     2221 ttgccccctt tcccggacat gtgtagaaaa tgaacctgga aaatggcagt ttttattgca
     2281 ctgcaaaatc accaggtttt aataattata atgattaatc attaaaatag cgcgtggggg
     2341 catgtgaaga gaacgtgaag aaaaaggcgt gattttggtt tccgggcggt ttttccgccc
     2401 gtggtagcgt gaagcgggtg aacactcgaa cttataatca cggagtgtac attagaggag
     2461 gtttctggcg ggagaaaggc tcccgcctgc ttctcacata acaatggctg attgaatgag
     2521 tccaaacgga aaggtgcaat acaatggcag aatcatttga ggtgccgctc tcaggggatg
     2581 agaagaccta catccctgac ccatcctaca aacagaatgc atggacgaaa gactatacca
     2641 ccgcatacaa ggaattcatg gcagatcccg atgggttctg ggagaagcag gcccaccact
     2701 ttgagtggat caggaagtgg gaccgcgtcc ttgagtggaa ctatccctat gcaaagtggt
     2761 tcgtcaacgc caagctcaat atcaccgcaa actgccttga ccgccatgtc aacaaccacc
     2821 gcaggaacaa ggtggccctc atctggaggg gtgaggaggg tgaggagcgg accttcacct
     2881 acaagaagct cttcggggag gtctgccggt ttgcaaacgg cctcaagaag ctgggcgtgg
     2941 caaaggggga cagggtctgc atctacatgc ccctcgtccc cgagcaggtg atcgcgatgc
     3001 tcgcatgcgc ccgcatcggc gcagtgcaca gcgtggtctt cggaggattc ggggtgcagg
     3061 cactcaacat gcgcatcaag gacgccgagg ccaagattgt cattaccgca gatgtcacgt
     3121 accgccgtgg gaagaccatt ccgttgaaga ccatcgtaga agaagccgtt atcaacgccc
     3181 cgagtgtgga gaaggtcgtg gtgctctccc gcgaggtcga gcagcccgtg gaaattcaca
     3241 aggagatgga ggtggactat ttcgagctga tggaaggagt gcctaccacc tgcgaggccg
     3301 aagtgatgga tgccgaggac cccctcttca tcctctatac cagcggatcc acggggactc
     3361 ccaagggaat agtccacacc tgtgcaggat acatggtcgg gaccgcctat accacccagc
     3421 acgtctttga tgtcaaggac aacgacgtct actggtgtac cgccgacacc gggtggatca
     3481 cggggcatag ttacgtcgtg tatgggccgc tcgccatagg agcaaccatt gtcctcgccg
     3541 aagcagtgcc tgactatccc gacgccgggg cattctggaa gattgtcgaa gaccttgggg
     3601 tcactatctt ctacaccgct cccacggcta tccgcatgtt catgaaggtg ggggagcagt
     3661 ggcccaacaa gtacgacctc tcatccctgc ggatcctggg atcggtcgga gagccgctga
     3721 accccgaagc attcgaatgg tattaccagg ttatcgggaa gaaaaaatgc cccatcgtgg
     3781 atacctggtg gcagaccgag accgggatgc aaatgatcac caccatggtg ggagaaccga
     3841 tgcgccccgg ttttgcgggg agaccgatcc caggtgtggt tgcggatgtc gtggacaaga
     3901 agggcaaacc cgtcgcaccc ggtaacggcg ggttccttgt gatccagagg ccatggccgg
     3961 ccatgctccg gacggtctac aagaacgatg aacgttaccg cacctactgg aacaccattc
     4021 cggactgcta caccgccggc gacctcgcag tgaaggcaaa gggcgacgag tatatcatga
     4081 tcatcgggag agcagacgac atcatcatcg tggcaggcca taatatcgga accgcggagg
     4141 tggagagcgc actggtctcc caccagtccg ttgccgaggc ggcagtgatc ggaaagccag
     4201 agcccatgaa ggggaacatc atcaaggcat tcgtgatcct gcgggtcgga ttctccccga
     4261 gcgagaagct caagtccgac ctcatctacc acgtccgcat gacacttggg cccatcgcca
     4321 tgccctccga gatcgagttc gtggacaagc ttccgaagac ccgctccgga aagatcatgc
     4381 gccgggtcct gaaagcgcag gaaatgggaa tagaccccgg ggacgtctcc acgctggagg
     4441 aatgattccc ccctttttcc ggatcgaaaa ggactgttat gcctgacgat gggagcatgt
     4501 tcggtatgcc cgcggagaaa gggcgctggg tccttgtatt cctcgggatg gccatcaacc
     4561 tctgccttgg gagcatctac tcctggagcg tctttgtcaa ccccctgact gactacttca
     4621 ccggtagcct tgggcagtcg gtcaccgcga acgaggtgct gatgcctttc tctgtcttcc
     4681 tggccttctt tgccatcgcg atgcccctgg cagggaagta catcgagaca ctcgggccaa
     4741 ggaacgtcac gattatcggt ggagtcctca ccggcctcgg gtggctgctt gcttcgatgg
     4801 tcacctctgt ccagatgctc tacctggtct acggggtgat cgggggagtg ggtgtcggga
     4861 tcgcctatgg ggtccctgtt gccgtcgccg cgaggtggtt tcccgacagg cggggacttg
     4921 ccgtcgggct cacggtgctc gggttcgggt tttctgcatt cctgaccgca aacattgcgg
     4981 ggtacctcat ctcctccttc ggggtgatgg acactttccg catctttggg atcgcgttcg
     5041 tggtgctgat catcctcctg gccctcccgc tccggtttcc tgcgcccggc tggaaacccc
     5101 gcggatggaa taccccggtg ccgaagcccg gagaggagac ttgcgagtgt gacaggggtg
     5161 cgatgctccg cacccccacg ttctacggcc tctgggcctg cttcttcatc ggctgcctgg
     5221 cgggccttat ggcgatctcc atcgcaaaac ccgtcgggac cgagatcgga atcgaggcgg
     5281 ggctcgccac cctgctcgtg ggattatttg ctatattcaa cgggggtggc cggccggtct
     5341 tcgggagcct caccgaccgg atcaccccga gaaatacggc gcttgcctct ttcgtcctca
     5401 tcgcactcgc atcgctt
//
LOCUS       DAOQVJ010000042         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_16935_length_5345_cov_3.240076, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000042 DAOQVJ010000000
VERSION     DAOQVJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16935_length_5345_cov_3.240076"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1508)
                     /gene="serA"
                     /locus_tag="PKL26_01605"
                     /pseudo
     CDS             complement(55..1508)
                     /gene="serA"
                     /locus_tag="PKL26_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866439.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            1654..>2495
                     /locus_tag="PKL26_01610"
     CDS             1654..>2495
                     /locus_tag="PKL26_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNS82421.1"
                     /translation="MKKMPDILWLEEIRKEDIAAVGGKGASLGEMSAIGLPVPRAFVV
                     TSNAFRRFLIESGIEKALFAGLANLDVEDSGELEKAANKAKELVMKARVPASIKQKVK
                     EAYLKMEGGLMVVAVRSSATAEDLPDASFAGQQETFLNVKGEKNLLLAMQKCWASLYG
                     ARAIYYRVKQGFDHQSVNIAVVVQQLVNAEKAGVMFTSHPVTGEPLSIIEGSWGLGEA
                     VVSGSVSPDKYVYDQRSEKVIDRLIANKRYQILSDGDNGTKTVEVERERQDTPLLSDE
                     EVRKL"
     assembly_gap    2496..2595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2596..4079
                     /locus_tag="PKL26_01615"
     CDS             <2596..4079
                     /locus_tag="PKL26_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNS82422.1"
                     /translation="ERERQDTPLLSDEEVRKLATFGRIAETHYGVPQDVEWATVGDDI
                     YILQSRPITTIKPPSASTPVQEAGAGKIILQGQGASPGIASGNVVIIRDAKDTGGVKE
                     GDILVTRMTNPDMVPAMRKVAAIITDEGGMTCHAAIVSRELGTPAIVGTKTSTQVLRA
                     GQTVTVDGEKGFIYEGRLEVPREKVQAAVTAAVPLITATSVKVNVSMPEAAARAAATG
                     ADGVGLLRIEHLILGLNKTPGWFIANNREEEFIQELYNGIKTVLDAFYGKPVWVRTLD
                     APTDEFRNMEGGEHEPIEHNPMLGWRGIRRDLQSPDQFRLQVETFKRLWTQGYDNLGI
                     MFPMVSHPSEFTRAREMMQQWGVDVESATLGVMIEIPSSAILIEDFIRAGIRFASFGT
                     NDLVQYTLAIDRNNEHVAGMYQPKHPAVLALIDSAIRCCREHGVEVSICGQAGSEPPM
                     VKWLVEHGITSVSANIDAIAKIRETVARTEQQILLESARSRNA"
     gene            4072..5169
                     /gene="mfnA"
                     /locus_tag="PKL26_01620"
     CDS             4072..5169
                     /gene="mfnA"
                     /locus_tag="PKL26_01620"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HNS82423.1"
                     /translation="MREIGLSEESLFSFLLSKREEDVDHAHILSSMCTIPHPVAVRAH
                     VQFMETNLGDPGLFPGTASLERLLVERLGTLFQLSGACGYATSGGTESNIQALRMARQ
                     LGPKEKPNVIIPESAHFSFKKACDILCLEMRAVPLDENYRMDPVAAAEMIDRNTCGIV
                     GIAGTTEYGMVDPIAELSKIAGDHGVFFHVDAAFGGLVLPFLDDAPAFDFSLPGVSSI
                     AVDPHKMGMSTIPAGVLLVRDSESLCSLSVDTPYLTVKQEFTLSGTRPGASVAGALAV
                     LEYLGIQGLRDIVAGCMKNTRRLIDGMEAYGIMRAATPDVNVATFSPARIPPPWKVSW
                     TRRGDLRLVCMPHVHAGAVESFLKDIGEQNA"
     gene            5162..>5345
                     /locus_tag="PKL26_01625"
     CDS             5162..>5345
                     /locus_tag="PKL26_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNS82424.1"
                     /translation="MLERLVASLETCPLVKRGEYNYFIHPITDGVPDVDPHLLREVCM
                     AMIKVLDLEGIDRIMAA"
BASE COUNT         1099 a         1531 c         1548 g         1067 t
ORIGIN      
        1 gtccacgttc aggaccatga tgacctcttc tccgggtttg atccgtccga cctacatacc
       61 ggcaatattc acattcccct tgccaaggat ggtcgaggcg cggccgatta cgcctggctt
      121 gtcgaggtgg cgggagacga tgacgtaccc ctcggggatc atgtccatcg tgtatcctcc
      181 tatggctacg atccgtgacc gacccttgta aaagaccgtg ccgctcatgg tctcctccat
      241 ggtgtcggtc ttcacccgga tggtgacaag gctcttccat ccacccgagt ccggggtcat
      301 tgtctcagat acgctgatcc ctcgctcctt ggcaatgaac tcggcattga cgatgttgat
      361 gggtacctgg agtatggggt cgaggaggcc ctttaacgcc atgagagtga tgaagcgggt
      421 attgttccca agcgtcgcaa gctccccgct gtaggtcagc tctaccttct ccatcctccc
      481 acccaccatc tgggtgagga acctgcccat cttgtgggcc aggatggcaa acggttcgat
      541 gacgtcggcg tgctcggcag ggacgattgg cgcatttacg atatagcgcg ccgaacctcc
      601 cttcaggacc gagaggcact gcctggcgat agagactgcc acgttctgct gggcctcgac
      661 cgtgctcgcg ccgagatgcg ggttgtgatt acctggtcaa gttcgagaag gggcgagttg
      721 agtggcggct cctcttcgaa gacatcgagg gccgcacctg cgaccttccc agatttgatc
      781 ccttcgtaaa gagccttctc gtcaatgatc ccgcccctgg cacagttgat gatccggacc
      841 ccgtctttca tggtggcgat ggttttggta ttgacgatgt gtttggtctc cttgatcagg
      901 ggggtatgaa ccgtgatgaa atccgagacc ttgaagaggt cttcgagcga gagtgcttcg
      961 actcccagct gggcggcacg gtccttgctg atgaaaggat cataccccac gacatgcatt
     1021 cccatcgcct gggcacgttt cgcaacttcc ctcccaatcc tcccgagacc gacgatcccg
     1081 agcgtcttct cgttcagttc cactcccatg aacctggagc gcttccattc cttcttttta
     1141 agcgaggcgt tcgcctgggg tatgcttcgt gcaagcgaaa gcatcatcgc catggtatgc
     1201 tcagtggcgg cgagagtgtt tccttccggc gcattggcaa cgatgatccc ctttctcgtt
     1261 gcggcatcca tatcgatatt gtcgacgccc gctccggccc tcccgataaa acgcagattc
     1321 ttcccggcct cgatcacccg cgaggtcacc tcggtcccgc tccgcaccag cagggcgtca
     1381 tactcgccga tgatggagac gagcgtatcc tccccaaggt cggtcttcac atcgacagtg
     1441 cactcctgtc gcaggatctc cagcccttcc tcggccagcg ggtcgctgac cagtactctg
     1501 aacgtcacta taaaacccac attatatatg catcaaacta attgagcgtt tttgtctccc
     1561 tgcgttattt ctcccatgga tgacatccag gtctcttcac aggtgatgag aatgtattaa
     1621 acagggaggg gtgcagatta tagtggtgcg tgaatgaaaa agatgcccga tattctctgg
     1681 cttgaggaga tacggaaaga ggatattgct gcagtcgggg gaaaaggtgc gtccctcggg
     1741 gagatgtctg ccatcggtct ccctgtcccg cgggcgttcg tggtcaccag caacgccttc
     1801 cgccggttcc tgatcgagag cggcattgaa aaggccctct ttgcaggcct tgcaaatctc
     1861 gacgtggaag attccgggga actcgagaag gcagcaaaca aggccaagga gttggtgatg
     1921 aaagcccgcg tccctgcatc aataaagcag aaggtcaaag aggcttatct aaagatggag
     1981 ggcggcctga tggtggttgc cgtccggtca agtgcaaccg cggaagacct gcccgacgca
     2041 agtttcgcag ggcagcagga gacctttttg aatgtaaagg gcgagaagaa cctccttctc
     2101 gccatgcaga agtgctgggc ctcgctctat ggggcaaggg caatctatta ccgggtcaag
     2161 cagggcttcg accaccagag cgtgaatatc gcggtggtgg tgcagcagct ggtgaacgca
     2221 gagaaggcag gggtgatgtt cacctctcac ccggtcacgg gagagccttt gagcatcatc
     2281 gaagggtcct gggggctcgg cgaggctgtc gtctcgggct cggtctcccc tgataaatac
     2341 gtatacgacc agcgcagcga gaaggtcatc gaccggctca tcgcgaacaa gcggtaccag
     2401 attctctctg acggggataa cggaacaaag accgtcgaag tggagcggga gcgacaggac
     2461 acccccctcc tctccgatga agaggtgaga aagctnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnntggag cgggagcgac aggacacccc cctcctctcc gatgaagagg
     2641 tgagaaagct tgccacattc ggcaggatcg ctgagaccca ctacggtgtc ccgcaggacg
     2701 tggaatgggc gaccgtggga gatgacatct atatcctgca gtcgcgtcca atcaccacga
     2761 tcaagcctcc ttcggcatca acgcccgtcc aagaggccgg ggccgggaag atcattctcc
     2821 agggccaggg agcatctccg gggatcgcga gcggcaatgt cgtgatcata agggacgcaa
     2881 aagacaccgg gggggtcaag gagggagaca tacttgtcac ccggatgacc aacccggaca
     2941 tggtccctgc catgagaaaa gtcgctgcca ttatcaccga tgaaggcggc atgacctgcc
     3001 acgccgctat agtgagcagg gagcttggga cacccgcaat cgttggcacc aagacctcca
     3061 cccaggtgct gagagcaggg cagacggtca cggttgatgg tgaaaagggt ttcatttatg
     3121 aagggaggct tgaagtcccg agggagaagg tccaggctgc ggttacagcc gccgtccccc
     3181 tcatcaccgc taccagtgtc aaagtcaacg tctccatgcc ggaggctgcg gcccgggcgg
     3241 cggctacagg tgccgacggg gtagggctgc tccggatcga gcacctgatc ctgggcctga
     3301 acaagactcc cggatggttc atcgccaaca acagggagga ggaattcatc caggaacttt
     3361 acaacgggat aaagacagtc cttgacgcgt tctacggcaa accggtctgg gtaaggacgc
     3421 tggatgcccc cactgatgag ttccggaaca tggaaggcgg cgagcacgag ccgatcgagc
     3481 acaacccgat gcttggatgg agaggtatca ggagggacct ccagtccccc gaccagttcc
     3541 gcctccaggt tgaaacattc aaaagactct ggacgcaggg atacgataac ctgggcatca
     3601 tgttccccat ggtctcccac ccttccgagt ttaccagggc acgggagatg atgcagcaat
     3661 ggggtgtcga cgttgaatca gccaccctcg gggtgatgat cgagatcccg agcagcgcca
     3721 tcctgatcga ggacttcatc cgtgccggga tcaggtttgc atcctttggc acaaacgacc
     3781 ttgtccagta cacgctcgcc atcgaccgga acaacgagca cgtcgcaggg atgtaccagc
     3841 cgaagcaccc ggcggtcctt gcactgatcg attctgccat ccggtgctgc agggagcatg
     3901 gagtggaagt atccatctgc ggccaggcgg gctcggagcc gcccatggtg aaatggctcg
     3961 tggagcacgg gatcacgagt gtctctgcga atatcgacgc catcgcgaag atccgcgaga
     4021 ccgttgccag gaccgagcag cagatactcc tggagagcgc gagatcccgg aatgcgtgag
     4081 attgggctct cagaggaatc actcttttca ttcctcctct cgaagagaga ggaagatgtc
     4141 gaccatgcac atatcctgag ttccatgtgc actatcccac accccgttgc ggtcagggca
     4201 cacgtgcagt tcatggagac gaacctcggg gacccggggc tcttccctgg aaccgcatcg
     4261 ctcgagcgcc tccttgtcga gcggctcggg acccttttcc agttgtccgg cgcctgcggg
     4321 tatgccacat caggggggac cgagtcgaac atccaggccc tgcggatggc aaggcagctc
     4381 ggcccgaaag agaagccgaa cgtcatcatc cccgagtccg cccacttctc gttcaaaaag
     4441 gcctgtgaca tcctctgcct ggagatgagg gccgttcccc tggatgaaaa ttatcgcatg
     4501 gaccctgtgg ctgcggcaga gatgatcgac cgtaacacct gcggcatagt ggggatcgcc
     4561 ggcacgacag agtatgggat ggtagacccc atcgccgagc tctcgaagat tgcaggagac
     4621 cacggggtct tctttcacgt cgacgcggca ttcggagggt tggtcctccc cttccttgat
     4681 gatgcgccgg cttttgactt ctccctgccc ggtgtcagca gcatcgcggt cgacccgcac
     4741 aagatgggga tgagcaccat cccggctggc gtgctcctcg tgcgggactc tgaatctctc
     4801 tgctcgctat cggtcgatac cccatacctt accgtgaaac aggagttcac gctttcaggg
     4861 acccgtcccg gcgcctcggt cgcaggggcg ctggcagtcc tcgaatacct ggggattcag
     4921 gggctgcgcg atattgtggc aggctgcatg aagaatacac gcaggcttat cgacggcatg
     4981 gaggcctacg gcatcatgcg ggccgcgact cccgatgtga acgtggccac tttctcccct
     5041 gcccgcatcc cacccccctg gaaggtgtcc tggacacgga gaggggatct ccgcctggta
     5101 tgcatgccgc atgtgcatgc cggggcggtg gaatcgtttc tgaaggacat tggtgaacag
     5161 aatgcttgaa cgacttgttg catcactcga gacctgcccc ctcgtgaagc gaggggagta
     5221 taactacttc atccacccca tcaccgatgg tgtccctgat gtcgaccccc acctccttcg
     5281 ggaggtctgc atggccatga tcaaggtcct cgacctcgag ggtatcgacc gcatcatggc
     5341 cgcgg
//
LOCUS       DAOQVJ010000043         5325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_17012_length_5325_cov_4.442315, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000043 DAOQVJ010000000
VERSION     DAOQVJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17012_length_5325_cov_4.442315"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PKL26_01630"
     CDS             <1..772
                     /locus_tag="PKL26_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNS82425.1"
                     /translation="YHFMLNQGHDYDLFDAFRTVLRGHDIPPEVAGCKREMIKEVAEK
                     MKNARFVMIFFGMGCTHTDGRNHNIDIAISLTRDLNTHTKASIMAMRGHYNIAGPGQV
                     WSWQFGFPYCIDLSKGTHAHMNPGETSSIDLAMRDEIDCFVNVGTDAGAHFPIAAVKH
                     LKKHPFITVDPNICMASEIADLHIPVRVVGVEEPGVVYRMDNVPIQFKKVVDGPEGVP
                     SDEELFDKVYARMCELTGCEPIWVHAKEHAKAATSTEH"
     gene            774..2480
                     /locus_tag="PKL26_01635"
     CDS             774..2480
                     /locus_tag="PKL26_01635"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HNS82426.1"
                     /translation="MAEILIKNGFVIDPSRKINGDVADVAIKDDKIVDKVGSGARVID
                     AKGKVVMAGAVDVHAHVAGPKVNVGRLFRPEDKLFRSPNAQENYRMEMGFSVPSTFKT
                     GYDYARMGYAFVMEAAMPPLHSPHVHEEIHDTPIIDEAALPVFANNWFVMEYLKNNEI
                     ENSAAYVAWLIAATKGYGIKLVNPGGTAAWAWGLNCLGINDPVPFYDITPAEIITGLM
                     KTNEYLGLPHSIHVHQNSLGEPGNYTVTLDSLKLAEGVKARNKFGRDSVMHSTHIQFH
                     SYGGDSWANFESKAKEVMDYVNAQKNITVDLGCVTLDETTTMTADGPFEHHLNNLNHL
                     KWANVDVELETAAGVVPYVYNKNIKVCGIQWAIGLELGLFAKDPMRTFVTTDHPNAGP
                     FTRYPRIIKWLMSKKAREATLDSFKYKDKVIDATNIHSMDRELTLYEIAQMTRAGPAK
                     SLGLSHMYGGLGAGLDANVAVYDLNLKNMPSDPEMIERALLRASWFIKSGQIVVKDGE
                     IAGHGNKRTVWVNAKVKENPQVTRDIHEKFLKNYTVGIQNYPVWDSLAPNPLVIDVDV
                     EA"
     gene            2484..3287
                     /locus_tag="PKL26_01640"
     CDS             2484..3287
                     /locus_tag="PKL26_01640"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNS82427.1"
                     /translation="MAKITLKPFKTPELFLECPIISPDMFAGKTPAEIAELEVREGKT
                     TQKLGDYFGVTGSAGKDAGETEILIDGDCSRVKYIGSKMTAGSITINGNADMYIGGWM
                     KGGKIHVKGNVDSFCGIAMEGGDIIVDGNAQNYVGCAYRGDWRGMRGGTIHIHGNAGN
                     DIGTFMLGGTIIIEKNAFIHVSTHAEGGTVVIKGDVQGRVGGQMVKGDIYVLGNIKYM
                     LPGFKEIDRVKKEIAGETATFKHYIGDLGERHPKSKGKEIYANLFVRAS"
     gene            3366..4112
                     /locus_tag="PKL26_01645"
     CDS             3366..4112
                     /locus_tag="PKL26_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNS82428.1"
                     /translation="MTDPGIEFLEKTRYPDFSTVDLIMRVPEPPNELPVTENARVISL
                     PSPKKVKITDVPVREALENWKPPGFFSRSSITKNDLSFLLWCMQGVRKADGDSPMLRN
                     VPSSGSRYPIETFFVAGEVEGLETGLYRYLPLSHRIVVERLDSDIPFAMGTASMNFNL
                     ITRAAVTFLFVGIPYRSTWALGNRGYRSVLIEAGHICQALIMASSCIGCEVQPIDLFH
                     DQMMIQLARLDPKTQWPLLVGTVGRREREL"
     gene            4244..>5325
                     /locus_tag="PKL26_01650"
     CDS             4244..>5325
                     /locus_tag="PKL26_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82429.1"
                     /translation="MDGNIFFTSEPLTAERLSWLVDLLKYYTTRLFPESLHHHPRTPT
                     PPFTFFLLGDACNILIGREHQRSLEIFFRLPCFRCIFDQGDLHVRRISIEPFRIRYPG
                     QVIPIAPGDNLPGRSIWDCLMNAMGKTPGPPSIGFLQMRSPYMFQSSSCVVDLFRAAA
                     RTGISPEFYGYLDGVHAMHRDQRPPHHVNIGEALSDIYRVAFTKGLFPRYLICQESAA
                     SRGYCTFSGDNGRVVSASLIPQARIKSLDHIVSRFCMSHRIFSHTSFFVDVVVQRKIP
                     SVKFSAERKKPSLVILASHSPYGTEFTKGAISLAVACAHQNIPTRIVFIEDGVYTLTG
                     SESPAGMWADMDMHALIEATSRMDTL"
BASE COUNT         1309 a         1447 c         1462 g         1107 t
ORIGIN      
        1 ttaccacttc atgctcaacc aggggcacga ctacgatctc ttcgatgcgt tcaggaccgt
       61 tctgcggggt catgacatcc ctccggaagt agcggggtgc aagagggaga tgataaagga
      121 ggtcgcagag aagatgaaga acgcgcggtt cgtcatgatc ttcttcggga tgggatgcac
      181 ccacactgac ggtcgcaacc acaacatcga tatcgcgatc agcctcacgc gcgatctcaa
      241 tacccacacg aaagcctcga ttatggccat gaggggccac tataatatcg caggccctgg
      301 ccaggtctgg tcctggcagt tcgggttccc ctattgcata gacctgtcaa agggaaccca
      361 tgcgcacatg aaccccggcg agaccagcag tatcgacctt gcgatgaggg acgagattga
      421 ctgtttcgtc aatgtcggca cggatgcagg agcacacttc cccattgcgg cggtaaaaca
      481 cttgaagaaa cacccgttca tcacggtaga cccgaatatc tgcatggcga gcgagattgc
      541 ggacctccac atacccgtcc gggtcgtggg tgtcgaagag ccgggtgtcg tctatcgcat
      601 ggacaatgtg ccgattcagt tcaagaaggt ggtagacggt ccggaaggtg tcccgagcga
      661 cgaggagctc tttgacaagg tctatgcccg gatgtgcgag ctgacgggtt gcgagccgat
      721 ttgggtccat gcgaaagaac acgcgaaagc agcgacctca acggagcact aatatggcag
      781 agatcttgat caaaaacggg ttcgtgatcg atccctcgcg caagatcaac ggcgatgttg
      841 cggatgttgc aataaaagac gataaaatcg tcgataaggt tggttcaggc gccagggtga
      901 tcgatgccaa aggcaaggtg gtcatggccg gggcagtcga tgtccatgcc cacgtcgccg
      961 gtccaaaggt gaacgtcggc cggctgttca ggcctgagga caagctcttc aggagtccaa
     1021 acgcccagga gaactaccgg atggagatgg gcttctcggt cccgagcact tttaagaccg
     1081 ggtacgatta cgccaggatg ggctacgcgt tcgtcatgga ggctgcaatg ccccccctcc
     1141 actctcccca cgtgcacgag gagatccatg acaccccgat catcgacgaa gccgcacttc
     1201 ccgtctttgc aaacaactgg tttgtcatgg agtacctgaa gaacaacgag atcgagaaca
     1261 gcgcagccta cgtcgcatgg ctgattgcag cgacaaaggg gtacgggata aaacttgtca
     1321 acccgggcgg aaccgctgca tgggcatggg gactcaactg cctcggcatc aacgacccgg
     1381 tcccgttcta tgacatcaca ccagccgaga ttatcaccgg gctcatgaag accaacgagt
     1441 acctgggact gccccactcc atccacgtcc accagaacag ccttggagag cccggcaact
     1501 acacggtcac gcttgattca ctcaaactcg ctgaaggagt caaagccagg aacaagttcg
     1561 gccgcgattc ggtgatgcat tcaacgcata tccagttcca ttcctacggc ggcgattcat
     1621 gggccaactt tgaatcgaag gccaaagagg tgatggatta tgtcaatgca cagaagaata
     1681 tcactgtcga cctcgggtgt gtcacgctcg atgaaacgac caccatgacc gcggacggac
     1741 ccttcgagca ccacctgaac aacctgaacc acctgaagtg ggcaaacgtg gacgtcgagc
     1801 tcgagactgc cgcgggtgtc gtgccttacg tgtacaacaa gaatatcaag gtttgcggca
     1861 tccagtgggc gatagggctc gaacttgggc tttttgcaaa ggaccccatg cgcacgttcg
     1921 tcacgacaga ccacccgaac gccggcccct tcacgaggta tccccgcatc atcaagtggc
     1981 tgatgagcaa gaaggcgcgg gaggcaacgc ttgactcgtt caagtataag gataaggtga
     2041 tcgatgccac caacatccac agtatggaca gggagctcac attatacgag attgcacaga
     2101 tgacgagggc tggccccgcg aaatcccttg gcctctccca catgtatggc ggtctcggag
     2161 caggccttga tgcaaatgtc gcggtgtatg acctcaactt aaagaacatg ccaagcgatc
     2221 ccgagatgat cgagcgtgcc ctgcttcgtg caagctggtt catcaagtcc gggcagatcg
     2281 ttgtcaagga cggggagatc gccggccacg gtaacaagcg gacagtgtgg gtgaatgcga
     2341 aagtcaaaga gaacccgcag gtcacgaggg acatccacga aaagttcctg aagaactaca
     2401 ctgtgggaat tcagaattac cctgtctggg actctcttgc accgaacccg ctggtcatcg
     2461 atgtcgacgt ggaggcctaa aacatggcga agataacgct gaaaccgttc aagaccccgg
     2521 agctcttcct cgagtgcccc atcatctccc ccgatatgtt tgccggaaag acaccagctg
     2581 agatcgcgga actcgaagtc cgcgagggga aaacaacgca aaaactggga gattacttcg
     2641 gagtcacagg atctgccgga aaggatgctg gtgagacaga aatcctcata gacggcgact
     2701 gttcccgggt aaaatacatt ggatcaaaaa tgaccgcagg atcaatcacc atcaacggca
     2761 acgcagacat gtatattggc ggatggatga aaggcggaaa gatccatgtc aaagggaatg
     2821 tcgactcgtt ctgcgggata gcaatggaag gcggcgacat cattgtcgac ggcaacgccc
     2881 agaattacgt ggggtgtgcg taccgcggtg actggcgcgg catgcgcggc gggactatcc
     2941 acatccacgg aaacgccggc aacgacatcg gcacgttcat gctcggcggc accatcatca
     3001 tcgagaagaa tgcgttcata cacgtctcaa cgcacgccga gggagggact gtcgtgataa
     3061 agggtgatgt ccagggccgc gtgggcgggc agatggtaaa aggcgacatc tatgtccttg
     3121 gcaatataaa gtatatgctc ccgggcttca aggagatcga cagggtcaag aaggagatcg
     3181 caggggagac cgcgacgttc aagcattata tcggagatct gggagaacgc catccgaagt
     3241 caaaaggaaa ggaaatctac gccaaccttt tcgtccgggc atcttaataa aaaagatctc
     3301 tttttttcct tgatgttaaa cgtattaact ccgagcggaa ataaactact attgatggag
     3361 gacatatgac agaccccggc atcgaatttt tggaaaagac caggtacccc gatttttcca
     3421 ccgttgatct catcatgagg gtccccgagc ccccgaacga gctgccagtt acggagaatg
     3481 cacgggtgat atcgctgccc agtccgaaaa aggtaaagat tacggatgtg ccggtcaggg
     3541 aagcgctcga gaactggaag cccccgggat tcttctcacg gtcatcgatt acaaaaaatg
     3601 atctctcttt cctgttatgg tgcatgcagg gtgtccgaaa agctgacggg gattcgccga
     3661 tgctccgcaa cgtgccttca agcggttcaa gatacccgat cgagaccttc tttgtggcag
     3721 gtgaagtgga aggtcttgag accgggctct atcgctacct ccccctttcg cataggatcg
     3781 ttgtcgagcg gcttgattcg gatattcctt ttgccatggg gactgccagc atgaacttca
     3841 acctcatcac aagggcggcg gttacatttc tcttcgtagg cattccatac cgctcgacat
     3901 gggcattggg aaacagggga taccggagcg tcctcatcga ggcaggccac atttgccagg
     3961 cgctcatcat ggcctcatcc tgcatcgggt gtgaggtgca gcctattgac ctcttccatg
     4021 accagatgat gattcaactc gcccgactgg acccaaagac ccagtggcca ttgctggtgg
     4081 ggacggtagg gagacgcgag cgtgaactct gacatgcggc ggcttgttga agttgccgaa
     4141 tgcgcggggt atcgggacgc ccgttcctca cactcttgca gagatcaggc ccaaaggaag
     4201 ggagaagaga taagtcgtgc gtgttcggca tttttttaaa aaaatggatg gaaatatctt
     4261 ctttacttcc gaacccctga ctgcggagcg gctctcctgg ctggtcgatc tgctgaaata
     4321 ttacacgacc cgtctcttcc ccgagagcct tcatcaccat ccacggacac ccacgcctcc
     4381 cttcacgttc ttcctcctcg gcgatgcatg caatatcctg ataggccgcg agcatcagcg
     4441 ctccctggag atctttttca ggctgccgtg tttccggtgt atcttcgatc aaggtgacct
     4501 tcatgtacga aggatatcta ttgaaccttt caggataagg tacccgggcc aggtcatccc
     4561 catcgcacct ggagacaatc tcccgggtcg ttcgatatgg gattgcctca tgaatgccat
     4621 gggaaaaaca cctggtcccc catcaatcgg gtttctccag atgcgttctc cctacatgtt
     4681 ccagtcttcc tcatgcgtgg tcgatctctt ccgggcagca gccagaaccg ggatctcccc
     4741 cgagttctac ggatacctcg acggggtcca tgccatgcac cgcgaccagc ggccgccaca
     4801 ccatgtgaat atcggagaag cactctctga catctatcgt gtcgcgttca caaaaggcct
     4861 ttttccccgg tatctcatct gccaggagag tgccgcctcg cgtggttact gcaccttttc
     4921 tggggataat ggaagagtgg tatctgcatc tctcataccc caggcacgga taaagagtct
     4981 ggaccatatc gtatccaggt tttgcatgag ccacaggatc ttctcgcaca cctctttttt
     5041 tgtggacgtg gtcgtccaga ggaaaattcc aagcgtcaag ttttcagcag aacgaaaaaa
     5101 accgtcgctt gtcatccttg cgtctcacag tccctacggg actgaattca caaagggtgc
     5161 aatttccctt gctgtggcat gtgcccacca gaatatcccc acaaggatag tcttcatcga
     5221 agacggggtc tacactctca cgggctcgga gtccccggcg gggatgtggg cagatatgga
     5281 tatgcatgcc ctgatagagg caacatcccg gatggatact ctcga
//
LOCUS       DAOQVJ010000044         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_17055_length_5315_cov_4.557605, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000044 DAOQVJ010000000
VERSION     DAOQVJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17055_length_5315_cov_4.557605"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1553
                     /locus_tag="PKL26_01655"
     CDS             165..1553
                     /locus_tag="PKL26_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82430.1"
                     /translation="MVDEIDIREPDVVPVVNRGYVIFPDPGGKYSVVAVREHHIAEGR
                     RRVERLLTDRVAMPPDVALDAAIELWDETRLPVCLYLKNHGKVIFDDFWESVVGNDLD
                     DTTLVMKYLGYTQDDASETFPVKIPVNERAADALRLAMGLDLCTPKPRAGATAHLAVP
                     KAAIEMWREHAETDPSAQGLNYVVGHPITKASIRAYFDLADIVPVKMAFDAIHWKDVA
                     YGWYRHADDTAYINYGAFAPEYVLAVLMLGRYNVNTVIDAFAATIVHEVTHRAEWLTG
                     FAYEGRWVQTQTPRGIENIPEGSELMAVGAEYTGGYALQKYDADHVRYHLREAFNCTD
                     ALCDIQGSDAMMAFGSLMDRVELVYGGVLPKICGAYVPFKYGPYLNYNFRVACPEENV
                     GALKEEFSASDFDVSWTMETRRAVRIKAPPYEKMYADLIGDIFVPADDQTWIAQAYRE
                     YPDSYGHKNMPI"
     gene            1665..2180
                     /locus_tag="PKL26_01660"
     CDS             1665..2180
                     /locus_tag="PKL26_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82431.1"
                     /translation="MLRLHRHVPPAERKSQKEIADMYNVHPQTVSRAIKRAEEIYTEF
                     ERGVESGAIRRTTAVQKASPPPESDAGGALMLPQQFVDVLSDMNQMQSACRATGTFMA
                     TAAHAAYEGVTNETLPHDQRFKMVVTGVSALAGTLFEMYDGTRQIQRMMQSSAKPTMR
                     DVRSEEGEFNE"
     gene            2395..4167
                     /locus_tag="PKL26_01665"
     CDS             2395..4167
                     /locus_tag="PKL26_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82432.1"
                     /translation="MMKDMHAQKTGAGVVAPLQPPEPEEPQDDADTSPAWQEALKPQV
                     ATIPSPRMPNVSRLPVTPRPTTRPELADDDLADPSTAIRAERPKLERKYPPIRVVEKK
                     DPLAVLKEVINEAGLKDDVTYTLHRIADLMDDGWSTEDFVMTSKQYGVNDAVARGVAM
                     RFKLEWKSHLKQLEEQQRVLERASGPAMGPFSRDRTPGESEPLVPPQAPQYQQMAQQI
                     GMQMLQSNPMMQMWAQSNPRDFEEMVKKVVEQTLKVQQPQQQFGMNPMMGGMNPYMTQ
                     QVFPFGVNPMMGGMNPMMGMMGQGAYPYSPSPPPRRETMTEEKVADIVEQKMASAMNE
                     IKVVLAQIGGAQRPQQQDDPLLKQIVLNMLQQQNQKKEADPMVTMLLQHVLENAGGRG
                     DAIEAMLAQLLQETKKGQDSRSLDLDHLREMINLKSIEGELGLKQREFDDRRESRDLM
                     RQALNEGLTVVGQAFASTMMSRGMGGAPMSMQQQQPVKTEMLQTDDGNIVMRCRACNE
                     LIMAPPDAIEVICPFCNAQFPVVDAPDFGDDEGHAPEHVAEEAPVTEPAPRDETVPSE
                     SSAQQSARGRGRKTIKHEDAEQNE"
     gene            4177..4554
                     /locus_tag="PKL26_01670"
     CDS             4177..4554
                     /locus_tag="PKL26_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82433.1"
                     /translation="MTDFKSSIIAQVAIRQLRSLLNGAIGQFTPNDIRTAILTDQSIW
                     GGGEQEIRQYTAKIPGIDTFGKAFIAGVERDYGSVTNLVVMWLREDQKLKYGMIVNTP
                     GGSEWLDRQVTEILKGIGLKDGA"
     gene            4547..5089
                     /locus_tag="PKL26_01675"
     CDS             4547..5089
                     /locus_tag="PKL26_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82434.1"
                     /translation="MPEMPRVQQAVPASFPYNPQAMAVQAVGVVVSKVAMTLVDRVLY
                     PEPKLTDLRDEMVKIIDENEPKVQEVRAKRAQYLQEPAPAPQAPPVTTKSPDVTAYIE
                     QSLQALQQAQEATMCKVCKREIGDAARMVQTKLATIQNTDKVYRSMKELEAQGALPQG
                     AKWSTLKETERQLVRDYARD"
     gene            5091..>5315
                     /locus_tag="PKL26_01680"
     CDS             5091..>5315
                     /locus_tag="PKL26_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82435.1"
                     /translation="MGEVDGVGGASGAGIDSGSEEYCAIAFEEATMMCATPELVFVQA
                     SKFMGFGPEEIDEMLPLDATYREALSAGVGE"
BASE COUNT         1195 a         1419 c         1744 g          957 t
ORIGIN      
        1 tcggtttcaa ccgtaatcat cccaaaattc gctgctgtgt agatcggggg tgtcatctga
       61 tggtctcccg gactaggcta ttacaattcg ggtatataaa atgtggcaac cctacggaat
      121 gtttttatta tcgcgcccca cagatgcgtt gagaggttgc ttgcatggtt gatgagattg
      181 acattcgcga acccgatgtc gttccggtgg tgaaccgggg gtatgtcatc ttcccggacc
      241 caggtgggaa atattcggtg gtggcggtgc gggaacacca catcgctgaa gggcggagga
      301 gagtcgaacg cttactcacg gacagggtcg caatgccccc ggacgtggcg ctcgacgctg
      361 cgattgaatt gtgggatgag acccggctcc cggtgtgcct ctatctcaaa aatcatggca
      421 aggttatctt cgacgacttc tgggagagcg tcgtgggtaa cgacctggac gacaccaccc
      481 tcgtcatgaa gtatctcgga tacacgcaag acgatgcaag cgagaccttc ccggtgaaga
      541 tcccggtaaa cgagcgcgct gcagatgcgt tgcggcttgc catgggactt gacctctgca
      601 ccccgaagcc tcgcgcgggc gcgacggcgc atctcgcggt cccaaaagca gccatcgaga
      661 tgtggcggga gcatgccgag actgacccct ccgcccaggg gctgaactac gtggtcggtc
      721 acccgatcac aaaagcgtcg atccgggcgt atttcgacct agcagacatc gtgcccgtga
      781 agatggcatt cgatgcgata cattggaagg atgttgcata cgggtggtat cgacacgcgg
      841 atgacacggc atacatcaat tacggggcgt ttgccccaga gtatgtgctc gcggtgctga
      901 tgctgggtcg atacaatgtg aacacggtga tcgatgcatt tgcggcgacg attgttcacg
      961 aggtaactca ccgggccgaa tggctgactg ggtttgccta cgaggggagg tgggtccaga
     1021 cgcagacgcc ccgtgggata gagaacatcc cagaggggag cgagttgatg gcggtcgggg
     1081 ccgagtacac cggcgggtat gctctccaga agtacgatgc cgaccacgtc cggtaccatc
     1141 tccgggaggc attcaactgc acggacgccc tttgcgacat tcaggggtcg gatgccatga
     1201 tggcgttcgg gtctctcatg gatcgggtcg agttggtata tggcggggtg ctgccgaaga
     1261 tatgcggggc gtacgtgccg ttcaaatacg ggccgtatct gaattacaat ttccgcgttg
     1321 cgtgtccgga ggagaacgtg ggcgccctca aggaggagtt ctccgcatcc gactttgacg
     1381 tgtcgtggac catggaaacc cgcagagcgg ttcggatcaa ggcaccgccc tacgagaaga
     1441 tgtatgccga cctcattggc gatatcttcg ttcccgcaga tgaccagacg tggatagcac
     1501 aggcgtatcg agaatatcca gattcctatg ggcacaagaa catgccgata tgataacgag
     1561 ccccaggcgc gcccgtgtga acaaaccttt ttatactcac acgttcattt gctcatttga
     1621 tggcagcagg tgctcagaaa gtatcagaca aggacgcact tgacatgctt cgcttgcatc
     1681 gacatgttcc tccggcagaa cgcaagtcgc agaaggagat cgctgatatg tataacgtgc
     1741 atcctcagac ggtctcccgt gcgattaagc gagccgaaga gatatatacc gagttcgagc
     1801 gaggtgttga aagtggcgcc atccgccgca caaccgccgt gcagaaagca tcccctccgc
     1861 cggaatcaga cgcgggcggc gccctcatgc tcccgcagca gttcgtggat gtcctctcgg
     1921 atatgaacca gatgcagtcg gcgtgccggg cgaccgggac gttcatggcg accgcagcgc
     1981 atgccgcgta cgagggggtc acgaacgaga cccttcctca tgaccagcga ttcaagatgg
     2041 tcgtgaccgg ggtaagtgcg ctcgcaggga cgctctttga gatgtacgac ggcacccggc
     2101 agatccagcg gatgatgcag agttccgcga aaccgaccat gcgggacgtt cggtcggagg
     2161 agggagaatt caatgagtga cgatgaccgc tttgtagccc ggtgcttgcg tgggcacgag
     2221 acagtaatca cagcaactac gcttcgacct atgctggatg tgaacaagaa aggcactacg
     2281 tttgtgattc ggtgtccgca tgttgaagac ggggttatct gtggtgcgcc agcccagatc
     2341 gcccagcgag atgtgtcgcg actgttagga atcagcgcta gcgaggcgaa acagatgatg
     2401 aaagatatgc atgcccagaa gaccggcgcc ggggtggttg ctcccttgca gcccccggag
     2461 cccgaagagc cacaggatga cgccgatacg tctccggcgt ggcaggaagc cctgaaaccg
     2521 caggttgcga ccataccgtc gccccggatg ccgaatgtgt ccagactccc ggtgacgccc
     2581 aggccgacaa cgcgacccga actagcagac gacgacctag cggatccttc gacggccatc
     2641 cgggcggagc gtccaaagct cgagaggaag tatcctccga ttcgggtggt cgagaagaag
     2701 gatccccttg ccgtcctcaa ggaagtgatc aacgaggcgg ggttgaagga cgatgtgacc
     2761 tacaccctgc accggatcgc ggatctgatg gatgacggat ggtccaccga agacttcgtg
     2821 atgacctcga agcagtatgg tgtgaacgat gctgtggccc gaggtgtggc gatgcggttc
     2881 aagctcgagt ggaagtcgca tctgaagcag ctcgaagagc aacagcgcgt gctcgaacgg
     2941 gcgagtggtc cagcgatggg gccgttctcc cgggaccgca ctccgggtga gagcgagccg
     3001 ctggtgccgc cgcaggcacc gcagtatcag cagatggccc agcagatcgg gatgcagatg
     3061 ctccagagca acccgatgat gcagatgtgg gcgcagagca acccgcggga tttcgaagag
     3121 atggtcaaga aggtcgtgga acagaccctg aaggtgcagc agccccagca gcagttcggg
     3181 atgaacccca tgatgggtgg catgaacccc tacatgacac agcaggtgtt cccgttcggc
     3241 gtgaatccga tgatgggcgg catgaacccg atgatgggaa tgatggggca gggggcctac
     3301 ccgtattcgc cgtctcctcc acctcggcgg gagaccatga cggaagagaa ggtcgcggac
     3361 atcgtagagc agaagatggc ctcggcgatg aacgagatca aggtggtgct ggcacagatc
     3421 ggaggtgcgc agcggccgca gcagcaggac gacccgctct tgaaacagat tgtcctgaac
     3481 atgctccagc agcagaacca gaagaaggaa gccgacccga tggtgaccat gcttctgcag
     3541 catgtgcttg agaacgccgg cggaaggggg gatgcaatcg aggcaatgct ggcccaatta
     3601 ctgcaagaaa caaaaaaagg ccaggactca cgctccttgg accttgacca cctccgggag
     3661 atgatcaacc tcaaatccat cgagggggag cttggtctca agcagcgtga gttcgatgac
     3721 cggagagaga gccgagacct gatgcggcag gcactgaacg aggggctgac ggtggtcggg
     3781 caggcgttcg cgtccacaat gatgtcccgc ggcatgggtg gtgccccgat gagcatgcag
     3841 cagcagcagc ccgtgaagac ggagatgctt cagaccgatg atggcaacat cgtcatgcgg
     3901 tgccgggcct gtaacgaact gatcatggcg ccgccggatg ccatcgaagt catctgtccg
     3961 ttctgtaacg cccagttccc ggtcgtggat gccccggact ttggtgacga cgaggggcat
     4021 gcacctgagc atgtggccga ggaggccccg gtgaccgaac ctgcgccgag ggatgagact
     4081 gtgcccagcg aatcgtcggc acagcagtct gctcgcgggc ggggaaggaa aaccattaaa
     4141 catgaggacg cggaacagaa cgagtagggg cacagcatga ctgatttcaa gtcgagtatc
     4201 atcgcccagg ttgcgatacg gcagctccgg tcgctcctga acggggcaat cgggcagttc
     4261 accccgaacg atatccgcac cgcaatcctc acggaccagt cgatctgggg cggcggcgag
     4321 caggagatcc ggcagtatac tgccaagatc cccggcatcg atacgttcgg gaaggcattc
     4381 atcgccggtg ttgagcgaga ctacgggagc gtcacgaacc tggtggtgat gtggctgcgg
     4441 gaagaccaga agctgaagta tgggatgatc gtgaacaccc ccggcgggtc ggagtggctt
     4501 gaccggcagg tgaccgagat tctcaaaggg atcgggctga aggacggtgc ctgagatgcc
     4561 tcgggtgcag caggcggtgc ctgcgtcatt cccctacaac ccacaggcca tggctgtgca
     4621 ggcggtgggt gtggtggtaa gcaaggttgc catgacgctg gttgaccggg tgctctaccc
     4681 ggagcccaag ctcaccgacc tccgggacga gatggtcaag atcatcgatg agaacgagcc
     4741 gaaggtgcag gaggtccggg caaagcgggc acagtatctg caggaacctg cacccgcccc
     4801 acaggctccg ccggtgacca cgaagtcgcc agatgtgacc gcctatatcg aacaatcgct
     4861 tcaggcgttg caacaggccc aggaggcaac gatgtgtaag gtctgcaagc gggagatcgg
     4921 ggatgcggca cggatggtcc aaaccaagct cgccacaatt cagaataccg acaaggtata
     4981 ccggtcgatg aaggaacttg aagcacaggg cgctttgccc cagggggcga agtggagcac
     5041 gctgaaagag accgagcggc aactggtccg ggactatgcg agggattgac atgggagaag
     5101 ttgatggtgt aggtggcgcg agtggcgccg ggattgactc gggctcagag gagtactgtg
     5161 caatcgcctt cgaggaggcg acgatgatgt gtgcgacgcc cgaactcgtg ttcgtgcagg
     5221 cgtcgaagtt catggggttc ggcccggagg agatcgatga gatgctcccg ctggatgcca
     5281 cctatcggga ggcgctgagc gccggggtcg gggag
//
LOCUS       DAOQVJ010000045         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_17242_length_5274_cov_4.187584, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000045 DAOQVJ010000000
VERSION     DAOQVJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17242_length_5274_cov_4.187584"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..631
                     /gene="pdxT"
                     /locus_tag="PKL26_01685"
     CDS             38..631
                     /gene="pdxT"
                     /locus_tag="PKL26_01685"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNS82436.1"
                     /translation="MGVRLGVLALQGDVSEHVRGFQAALSQIGAKGGEVVPVRQPADL
                     AGLSGLAIPGGESTTISRLIQKNGLYEPLIRFEGGIFATCAGMVLMASRVDDPRVKTL
                     GLLDMTVLRNAFGRQKESFEAEIPIKGLDTPYHAFFIRAPVATGTGKEVEVLARIDAG
                     IVAVRKGPHMALSFHPELGNDIRLHRMFLAGLGIGKP"
     gene            complement(821..2851)
                     /locus_tag="PKL26_01690"
     CDS             complement(821..2851)
                     /locus_tag="PKL26_01690"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNS82437.1"
                     /translation="MDMFFIVPVCALLGLVFAGYSFMVMKREGTGTELMQKIAGAIRL
                     GAMVYLKSQYKAIAVFMVVLAIVLYLAPGIHPLTSVAFLVGGILSATAGFVGMTAATM
                     ANVRTTNAAREGMAKAFRVSYASGTVMGLTVVGLGLMGLSLLFLTLNALTTLGLSEII
                     NILSGFSLGASSIALFARVGGGIFTKAADVGADLVGKVEVGIPEDDPRNPAVIADNVG
                     DNVGDIAGMGADLYESYVGSIVATMLMGVSLAAATQFVGVASLNLVLLPMFIAGLGII
                     ASIIGSFFVRSNKNESSAIHKAFNAGTFASLILTVIAAYFLVTMVLGPQYIGVFVATI
                     AGLVAGFLIGLITEYYTSYERPPTRKIANSCETGAATNIITGLAVGFESTVFPVVIVV
                     VAILIAAQQAGLYGVAIAGVGMLATLGITLSVDAYGPVADNAGGIAEMSHQPKEVRGI
                     TDTLDAVGNTTAAIGKGFAIGGAALTALGLFAAYSQAVSLEIVNMLEPVVFVGVLIGA
                     MLPFLFCSFAMTAVGKAAGYIVQEVRRQFKEIPGLMEGKAEPDYSACITISTNAALKQ
                     MIAPGILAIAAPLVIGLLLGTHALAGLLVGSISTGFIVAVMMANTGGSWDNAKKYIEQ
                     GHMGGKGSPAHKAAVTGDTVGDPFKDTAGPALNILLKLMSIVAVVFAPMFLI"
     gene            complement(3077..4474)
                     /locus_tag="PKL26_01695"
     CDS             complement(3077..4474)
                     /locus_tag="PKL26_01695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HNS82438.1"
                     /translation="MLDAATIVQRFLATKMQVHPEVVRYLSEKDDPGLIDQIIASVPG
                     DTIVVSLQHIPGLHQDRDGARFLADPSCEVLRGAAGTTKSISHITDYCHYFRDRYQRL
                     GEYIRPRAAPMPIEALKKGARYRQEACSIMGLVMESKATTNGHRIVEIEDSSASISVL
                     FHKERPQFGEAETLVPDEVVAVRGTLSGDGRLFFAEQLFRPDIPLTHAPFRSQDPGSA
                     ILISDVHVGSNTFLEEAWNRFADWLSDSDATYLLIAGDLVDGIGVYPNQEQELTITNI
                     YEQYEVFGTMLRDLPSHMKIVICPGNHDVVRAAEPQPAIPSEFTANFPSNCIMVENPA
                     LVSLQGVRVQMYHGRSIDDFISLIPGASYENAAPMMMGMLQRRHLAPTYGKRTPIAAG
                     KEDALIIDPIPEVLHTGHVHIMGIGDYRGVLCVNSGTWQGQTAFQKQMNITPTPGRAV
                     VLDLQTLNTRVLDFT"
     gene            4639..>5274
                     /locus_tag="PKL26_01700"
     CDS             4639..>5274
                     /locus_tag="PKL26_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNS82439.1"
                     /translation="MSRNDPEENKPGLWEQFRKSENPWVSLARELAWVAAVVGGIALA
                     LFLVSGTWPAVVTIESESMVPHMQVGDLVFVVSADRFGGLESWTSGIEKGYLKYGDYG
                     DILIYRPNDAPNPPVYIPFLTKGVHPIIHRAMDRIDAGENIPKYYNLYRGQATPARYL
                     PATIQNNSLVLEDGTVVTPQNADPMNGYLVETTLVSPHAGYTTKGDNNQVSD"
BASE COUNT         1092 a         1528 c         1657 g          997 t
ORIGIN      
        1 gtccataccc tgcgcgagga cgaggtgctc cagtaccgtg ggcgttagac tcggcgtcct
       61 ggccctccag ggcgatgtga gcgagcatgt cagggggttt caggcggcgc tctcgcagat
      121 cggggcgaag ggaggagagg tggtcccggt caggcagcct gcagaccttg ccgggctctc
      181 tggccttgcc atcccgggag gcgaatcaac cactatctcc cggttgatcc agaagaacgg
      241 gctgtatgaa ccgttgatca ggtttgaagg cggaattttt gcaacctgtg caggcatggt
      301 gctgatggca agccgggtgg acgacccgcg ggtaaagacg ctcgggctcc ttgacatgac
      361 ggtgctccgg aacgcctttg ggcggcagaa ggaatccttc gaggccgaaa ttcccataaa
      421 aggcctggat acgccgtacc acgcattttt catacgcgct ccggtggcga ccgggacagg
      481 gaaagaagtc gaggtcctgg ccaggataga tgccgggatc gttgcggtga gaaaggggcc
      541 acatatggca ctctcgttcc atcccgaact agggaatgat atcaggctgc accggatgtt
      601 cctcgcagga ctcgggatcg gaaagccctg agagccacgg gcacggccag gcatttttac
      661 accattggtc acttgggcta tttgttctgc ggcccccggc tccctttgat ctcacgcaat
      721 aatgctctgg atcgccatcc tcttccagta tccccagccg tgctggaaaa ccattgtatc
      781 agaacaagga gagctctggc acctttaaaa aaaaaaagaa ttagatgagg aacatcggtg
      841 caaagaccac cgcaacgatc gacatcagct tcaggaggat gttgagtgcc ggtcccgcgg
      901 tgtccttgaa ggggtcgccg acagtgtcac cggtgacggc cgccttgtgt gcgggcgaac
      961 cctttccgcc catgtgaccc tgctcgatgt acttctttgc attgtcccag gagcccccgg
     1021 tgttggccat catcacagcg acgataaagc cggtggatat ggagcccaca aggagccctg
     1081 cgagggcgtg tgtcccgagg agaaggccaa tcacgagggg tgcggcgatg gcgaggatgc
     1141 caggggcgat catctgcttg agcgcggcat tcgtggagat ggtgatgcag gcactgtagt
     1201 caggttcggc cttgccctcc ataaggcccg gtatctcctt gaactggcgg cggacctcct
     1261 gcacaatgta tcctgcggcc ttcccgacgg cggtcatcgc aaacgagcag aagaggaacg
     1321 gcagcatcgc gccgattagg acaccgacga agactacggg ctcgagcatg ttcacgatct
     1381 cgaggctgac agcctgggag tacgcggcga agaggccgag cgcggtcagc gcggccccgc
     1441 cgattgcaaa gcccttgcca atcgcggcag tggtgttccc cactgcgtca agggtatcag
     1501 tgatgccgcg gacttctttt ggctggtggg acatctcggc aataccgccg gcgttgtcag
     1561 cgacgggccc gtaggcgtcc accgagagcg tgatgccgag cgttgcaagc atcccgacac
     1621 cagcgatagc cacgccatag aggcctgcct gctgtgccgc gatgaggatc gcgacgacga
     1681 caatcacgac ggggaatacc gtgctctcga accccacggc aaggccggtg atgatgttcg
     1741 ttgccgcacc cgtctcgcag gagttggcga tcttccgggt tggcgggcgc tcgtaggagg
     1801 tataatactc cgtgatgagc cctatgagga acccggccac gaggccggca atggttgcaa
     1861 caaagacccc gatgtactgg ggaccgagca ccatcgtcac caggaagtac gcggcgatca
     1921 cagtgaggat gaggcttgcg aaggtccctg cattgaaggc tttatggatg gcgctgctct
     1981 cgttcttgtt gctcctgaca aagaagctcc cgatgatgga tgcgatgatc ccaaggcccg
     2041 cgatgaacat cgggaggagc acgaggttca gggaagcgac tcccacgaac tgtgtcgcag
     2101 ctgcaagaga gacacccatc agcatcgtgg caacaatgga tccgacgtac gactcgtaaa
     2161 ggtcggcgcc cattccggca atgtcgccga cgttgtcccc cacgttatcg gcgatgaccg
     2221 ccgggttccg cggatcgtcc tcagggatac cgacctcaac cttgcccacc aggtctgcac
     2281 ccacatcggc ggctttggtg aagatccctc cgcccacacg ggcgaagagt gcgatggaac
     2341 tggcaccgag cgagaaccct gaaaggatgt tgatgatctc ggaaagccca agggtggtca
     2401 acgcgttcag ggtcaggaag aggagagaga gccccatgag gccgaggccg accacggtga
     2461 gccccatgac cgttccgctt gcatacgaca cccggaaggc ttttgccatg ccttcacgcg
     2521 cggcgttggt ggtccggacg ttggccatgg tggccgcggt catgcccacg aatcccgcag
     2581 tagcggaaag tatcccgcct accaggaatg ccaccgacgt gagcgggtgg atgcccggtg
     2641 cgagatagag gacgattgcc agcacgacca tgaataccgc gatcgccttg tactggctct
     2701 tgagatacac catggcccca agccggatag caccggcgat tttctgcatc agttcggtcc
     2761 ctgtgccctc ccgcttcatg accatgaagg agtacccggc aaagaccagg cccagcaggg
     2821 cacagacggg aacaatgaag aacatatcca tacctgtaaa acctccggag aatgtaccat
     2881 tctccacgca taccagatga ttatctgaga tgaatcgacc cgggcacata aaaaactaca
     2941 tgaaacccgt tgaagtggta tccggccgcc ggaaagggga gggggtttaa cctgggaaag
     3001 ggggccttct gatggacctt cgccgcgccg gcagatacat gcacccggac acagaatcct
     3061 gggaatgcag ccgcaatcag gtgaaatcga ggacccgtgt gttcagtgtc tggaggtcga
     3121 ggaccaccgc cctccctggc gtgggcgtga tgttcatctg cttctggaag gcggtctggc
     3181 cctgccacgt gcccgagttc acgcacagca ctccgcggta atcgccgatt cccatgatat
     3241 ggacgtggcc cgtgtgcaac acctccggga tggggtcgat gatcagggcg tcctctttcc
     3301 ctgccgcgat cggggtcctc ttcccgtacg tgggggccag gtgccgcctc tgcagcatcc
     3361 ccatcatcat gggtgcggcg ttctcgtatg aggcgccggg aatgaggctg atgaagtcgt
     3421 cgattgaccg gccgtggtac atctgcactc tcaccccctg gaggctgacg agcgcggggt
     3481 tctccaccat gatacagttg gagggaaagt tggcggtgaa ctcagagggt atggcaggct
     3541 ggggttccgc agccctgacg acgtcgtggt tgcccgggca gatgacgatc ttcatgtggg
     3601 agggaaggtc ccggagcatc gttccgaata cctcgtactg ctcgtagatg ttggtgatgg
     3661 tcagctcctg ctcctggttg gggtagacac cgatcccgtc cacgaggtcc cctgcgatga
     3721 ggaggtaggt ggcgtccgag tcggagagcc agtcggcaaa gcggttccag gcctcttcga
     3781 ggaacgtgtt gcttccgacg tggacatcgg agatcaggat cgcagacccg ggatcctggc
     3841 tcctgaaggg tgcatgggtg agggggatgt ccggcctgaa gagctgttcg gcaaagaaga
     3901 ggcggccatc gccagagagt gtccccctca ctgccacgac ctcgtccggg accagggtct
     3961 cggcctcccc gaactgcggg cgttccttgt gaaaaaggac ggagatcgac gcggatgagt
     4021 cttcgatctc cacgatgcgg tgaccattcg tagtcgcctt ggactccatc acgagtccca
     4081 tgatggagca ggcctcctgg cggtaccgcg cccccttctt cagcgcctcg atgggcatcg
     4141 gcgccgcccg tggcctgata tactctccca ggcgctggta ccggtctctg aagtaatggc
     4201 agtaatcagt gatgtgcgag atgctcttcg tcgttcctgc ggccccccgg agcacctcgc
     4261 atgaagggtc tgccaggaac ctggcgccgt cacggtcctg gtggagcccg ggaatgtgct
     4321 ggagggatac gacgatggtg tctcccggca cacttgcgat gatctggtcg atcaggcccg
     4381 ggtcatcctt ctcggagaga tagcggacca cctcgggatg cacctgcatc ttggtcgcaa
     4441 ggaaccgctg cacaatggtg gccgcatcga gcattgcatc aatattggct gtgccggata
     4501 taggactttc actccatgtc catgaagtgc gaaccgtgcc ctccgccggg aaattgtgtt
     4561 gagatgcgaa ccgccggcct gcggcaggca gcgtgcaatc ccttattagg cgccggagga
     4621 aatattcaaa gtccaacaat gtcgcgcaac gatcccgaag agaataaacc cgggctgtgg
     4681 gaacaattca ggaaaagcga gaacccctgg gtttcgctcg caagggagct tgcgtgggtc
     4741 gcagcggtgg tggggggaat cgcccttgcg ctctttctcg tctccgggac atggcccgca
     4801 gtcgtgacga tcgagtccga gagcatggtc ccgcacatgc aggtagggga cctggtcttc
     4861 gtggtcagcg ctgaccggtt cggggggctg gagagctgga cgagcgggat tgagaagggc
     4921 tacctgaaat acggggacta cggggatatt ctcatctatc ggccgaatga tgcccctaac
     4981 cctcctgtct acatcccgtt cctgacaaag ggagtgcacc ctatcatcca ccgggcaatg
     5041 gaccgcattg acgcggggga gaacattccc aagtattaca acctctaccg cggccaggcg
     5101 accccggccc gctacctgcc ggcgaccatc cagaacaaca gcctggtcct cgaggatggg
     5161 acggtcgtta ctccccagaa tgcagacccc atgaacggtt acctggttga gaccaccctt
     5221 gtcagcccgc atgccggcta caccaccaag ggggacaaca accaggtaag tgac
//
LOCUS       DAOQVJ010000046         5174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_17715_length_5174_cov_3.887087, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000046 DAOQVJ010000000
VERSION     DAOQVJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17715_length_5174_cov_3.887087"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKL26_01705"
     CDS             <1..810
                     /locus_tag="PKL26_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNS82440.1"
                     /translation="PGALEHKGARIQILDIPGLIAGAAMGKGRGKEVIGVVRGADMIV
                     ILVDVFNANHVDVLMKELYDAGIRINRPRPDITIKKTALGGVRMNHVGILDLDVEEIR
                     SILSENKMMNADVLIRGNATQDDVIDAMLANRVYIPAFVAVNKVDLIDDTARKAIEEE
                     ILARFGDAPLLISAHTGYHMEEMKDEIYSRLGFMRVYLKPQSGAADLEEPLIIRSGST
                     VEDVCRKLHRDFVDRFRYARVWGHSVKHPGQRVGLPHRLREGDLLTIIIEH"
     gene            complement(831..1346)
                     /locus_tag="PKL26_01710"
     CDS             complement(831..1346)
                     /locus_tag="PKL26_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNS82441.1"
                     /translation="MKILVVIPPDQFRDEELAEPLAAFQNAGISFEVASTRRGTCKGM
                     LGTQAMANFSLEEVEPGEYAGIVIVGGSGSPAHLWDNEILINLVKYFHKNRKLVAAIC
                     LSPVVLAQAGILKERTATCYMSPASKREIIKGGAHISEKDVVVDDSIITANGPAAAKE
                     FAGAIIHHLKG"
     gene            complement(1456..2598)
                     /locus_tag="PKL26_01715"
     CDS             complement(1456..2598)
                     /locus_tag="PKL26_01715"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HNS82442.1"
                     /translation="MKYIEAEEGNTRFFIPVQDPSHPFPPASAPIFYNPRMELSRDAT
                     VVLVSLLSPSQYLDAMAASGIRGLRIASECRVPVTINDRDRGAFALIRRNAGLLGLPV
                     EVLHSDANVLLSGRCFEAVDIDPFGSPAPFVDSGIRGAGRYLFVTATDTAPLCGAHKN
                     AGIRRYFAQALNNEYHAETALRILLGFLVREAVKYDRGIEPLFCFSREHFVRVHVRLS
                     RGANAADRTVARIGYVMQCLSCPFRSEETGLLPEAGLCPDCGAGLIPVGPLWTGSLQD
                     REILEKMEEAIPAFRLAKSAQLLRLVNTCRQEPDISFHYDYHVLSRHFRLSPPPMEGV
                     IRSLVAGGYAAARAHYSGTALKTDAPLDVIREVLLAGRSGIDDQGI"
     gene            2658..2732
                     /locus_tag="PKL26_01720"
     tRNA            2658..2732
                     /locus_tag="PKL26_01720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2693..2695,aa:Arg,seq:cct)
     gene            2802..3662
                     /locus_tag="PKL26_01725"
     CDS             2802..3662
                     /locus_tag="PKL26_01725"
                     /EC_number="2.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNS82443.1"
                     /translation="MQEEGAILPYPPYLRVIRPLNALVAGCAAIIGYFLAAGTLVPGI
                     AVLFLVVFLVTGAGNAINDYFDVEIDRINRPDRPIPSGEASAKGVLVFSILLFLAGIA
                     LSFLTNIFCIVIAVANSLLLAWYAAALKRTPGAGNAAISYLTASIFLFGGAYAGPDGL
                     QLVSPVALITFFAMFAREIWKDAEDVLGDVASGADTLPIRIGVYPAICLGFVFLVGAL
                     LASLVPVLWWGPYYLAGIACVDILILCVGIRALGCKTAACLKKSRVTTLLKYAMFASL
                     AVFAVSAFLL"
     gene            complement(3816..4520)
                     /locus_tag="PKL26_01730"
     CDS             complement(3816..4520)
                     /locus_tag="PKL26_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNS82444.1"
                     /translation="MWQSLVFLIGEITIVVIIGIFFFSFILVALSIYSINTGRLYFPR
                     FLKAGMVLVEGLAKSLFRLFGLEDSEVLTFFINLHNTMNKKSFSEIPVTERAIFLPQC
                     LRSSRCPAHLSPEGLKCRSCGECTIGFWRKILEAMGYRVFIVPGSSFIKRMVKKYHPR
                     AILGVGCLSEVKEGLEMSDKMGLVAMGVVTLKDGCVETLVAWDKVMEVATLGVPPAGI
                     PPEAKEVNSWPVAEDY"
     gene            complement(4553..>5174)
                     /locus_tag="PKL26_01735"
     CDS             complement(4553..>5174)
                     /locus_tag="PKL26_01735"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNS82445.1"
                     /translation="ALFPEGKASSVLTHCNAGALACSEWGTALGVVRSAVESGAEVQV
                     IACETRPLLQGARLTAWELQRDGIPVTVNPDSSAAFLMRRGEIDMVIVGADRVTPDAV
                     FNKIGTYMHAVCASHHGVPFYVAAPLSTFDPHRIAREVTIEQRDRNEVAFCGSTVTVP
                     DGVGVKNYAFDLTPHQLVTAFITDIGVISPPVDWNEIEAARKDRGL"
BASE COUNT         1085 a         1504 c         1485 g         1100 t
ORIGIN      
        1 cccggcgccc ttgagcacaa gggtgcacgg atccagattc tcgacattcc gggcctgatc
       61 gcgggagctg ccatgggaaa gggccgcggc aaagaggtga tcggcgtggt gaggggagcc
      121 gacatgatcg tgatcctggt ggatgtcttc aacgcgaacc acgtggacgt cctgatgaag
      181 gagctttacg acgctgggat tcgaatcaac cggccacgtc ccgacatcac catcaagaag
      241 acagcacttg gcggggtccg gatgaaccat gtcgggattc ttgaccttga cgtagaggag
      301 atccggtcca tcctctctga gaacaagatg atgaatgccg atgtactgat ccgcgggaac
      361 gccacccagg acgatgtcat cgatgcgatg cttgccaacc gcgtatacat tcccgcgttt
      421 gtcgcggtga acaaggtcga cctcattgat gatacggctc gaaaagcaat cgaagaggag
      481 atcctcgcgc ggtttgggga tgcgccgctt ctcatctcgg cccataccgg ttatcacatg
      541 gaggagatga aggacgagat ctacagccgg ctcgggttca tgcgggtata cttgaagccc
      601 cagagcggcg ctgcagacct ggaggagccc ctcatcatca ggtccgggag caccgtcgaa
      661 gacgtctgcc gcaagctcca tcgcgacttc gtggacaggt tccggtatgc cagggtctgg
      721 ggccattccg tgaagcaccc agggcagcgg gtagggctcc cccacagact cagggaaggt
      781 gacctcctca ctatcatcat cgagcactaa aaagcctcaa aaacgactgt ttagcccttg
      841 aggtggtgaa tgatggcccc tgcaaactct tttgccgcag cggggccatt tgcggtgatg
      901 atgctgtcgt ctaccactac gtctttctcc gagatatgcg cccctccttt tattatctct
      961 ctttttgatg cagggctcat gtagcaggtt gcagtccttt ccttcaggat ccctgcctgg
     1021 gccagcacta caggggagag gcagattgcc gcaaccagtt tccggttctt atggaagtac
     1081 ttcaccagat tgatcaggat ctcattgtcc cacaggtgcg caggagagcc cgatcccccg
     1141 acaatgacaa tgcctgcata ctctccaggt tccacctctt cgagggagaa attcgccata
     1201 gcctgcgtgc cgagcatccc cttgcacgtc cccctccggg tggaagcaac ctcaaaggag
     1261 atccctgcgt tctgaaacgc agccaatggt tcagccagct cctcgtcgcg gaactggtca
     1321 ggtggaatca cgaccaggat cttcatacag ggaaaggttc tccatcaggg ataagaatgt
     1381 ttcccgcagg gtggacctga aaggcgggtg aatgggagga aggtggccag gtggggcatc
     1441 tcggatatga agggatcaga tgccctgatc atcgatgccg ctcctgcctg caaggagcac
     1501 ttcccggatc acatcgagag gcgcatccgt tttcagcgca gtcccgctgt agtgggccct
     1561 cgcggcggca tatcctcctg ccacaagcga ccgaattact ccctccattg gtggcgggga
     1621 caacctgaag tgccgcgaga gcacgtggta atcgtaatgg aaagagatgt cgggttcctg
     1681 cctgcaggtg ttcaccagtc gcagaagctg cgctgacttt gcaagtctga acgcagggat
     1741 cgcctcttcc atcttctcca ggatctccct gtcctgcagg gacccggtcc agagcggccc
     1801 gacgggtatg agtccggcgc cgcaatccgg gcacaacccg gcctccggga gcaacccggt
     1861 ctcttccgac cggaaaggac acgaaaggca ctgcatgaca taaccaatcc gggcaacggt
     1921 tcggtctgcg gcgtttgccc cccgggagag cctgacgtgc acacgcacga agtgctcgcg
     1981 cgagaaacaa aagagtggct cgataccccg gtcatacttc accgcctccc ggacaaggaa
     2041 tccaaggaga atccgcaacg ccgtctctgc atgatactcg ttgttcaggg cctgggcaaa
     2101 atagcgccgg atgccggcat ttttgtgggc accacagagg ggggccgtat cagttgccgt
     2161 cacgaatagg tacctgcctg cccccctgat ccccgagtcc acaaatggcg ccggggaacc
     2221 aaaagggtcg atatcaaccg cttcaaagca cctcccggag aggagcacgt tcgcatccga
     2281 gtgcagcacc tctacgggga gtcccaggag tccggcattc cggcgaatga gcgcaaacgc
     2341 cccacggtcg cgatcattga tggtcacagg caccctgcat tcagaggcga tgcggagccc
     2401 cctgatcccg gaggccgcca tggcatcaag gtactgcgac ggcgaaagga gagagacgag
     2461 gacaacggtg gcgtcgcggg agagttccat ccgcgggttg tagaagatcg gggcggaggc
     2521 gggaggaaac gggtgggatg gatcctgcac agggataaaa aaccgcgtat ttccctcttc
     2581 tgcctcgata tacttcattg ccgcaaatgt ggcggttcga cagccttata ccttctcctt
     2641 aataatgaat ataggcaggg cgtgtggcct agtcaggaca gggcggcagc ctcctaagct
     2701 gtaggtcggg ggttcaaatc cccccacgcc cgtttctctc tcgtttttct cctcattact
     2761 aacgatatta atacttcccg ggttgcactt agagaccata tgtgcaggag gaaggagcca
     2821 ttctcccgta tcccccgtac ttgagggtga tccgcccgct caacgcgctt gttgccgggt
     2881 gtgcagccat catcggctat ttcttggctg ccggcaccct tgttccgggt atcgccgtcc
     2941 tcttcctcgt cgtgttcctc gttaccgggg caggaaatgc catcaacgac tattttgacg
     3001 tggagatcga ccgcatcaac aggcctgacc gccctatccc atcaggagag gcaagcgcga
     3061 agggagtgct tgtcttctcc attcttcttt tccttgcagg aattgcgctc tcgttcctga
     3121 caaacatttt ctgcattgta atcgccgtcg cgaactcgct tctcctcgca tggtatgctg
     3181 ctgccctgaa gaggacacct ggggcaggga atgccgccat ttcatatctc actgcgtcga
     3241 tcttcctgtt tggcggggca tatgccggcc ccgacggcct gcagctcgta tctccggtgg
     3301 ccctgatcac cttctttgcc atgttcgcac gggagatctg gaaggatgca gaggatgttc
     3361 tgggggatgt tgccagtggt gccgatactc tccctatcag gatcggagtg tatcctgcaa
     3421 tctgccttgg atttgtattc cttgtgggag cgcttcttgc aagccttgtg cctgtgctct
     3481 ggtgggggcc gtattacctg gccgggatcg cctgcgtcga tatcctcatc ctctgtgtgg
     3541 gtatcagagc cctgggctgt aaaaccgctg cctgcctgaa gaaatcccgg gttaccaccc
     3601 tcttgaagta tgcgatgttt gcgtctctgg cggtattcgc cgtgtccgca ttccttctct
     3661 gaacatgccc ctgtcaaaga cttcctcact tcaaccatcc cctgcaggga cgattcagat
     3721 gtgcagacgg gaaaacgaga tagagagttg cggtgaaaaa aggatgccgc gtctgcatat
     3781 ccggttattc tcatcgtgaa tgtgtgtgcg ggacttcaat aatcttctgc gaccggccag
     3841 ctgttcactt cctttgcctc gggagggatc ccggccgggg gcacgccgag ggttgcaacc
     3901 tccatcacct tgtcccaggc gacgagtgtc tccacacacc catccttgag cgtcaccact
     3961 cccatggcca caagccccat cttgtcggac atctcgaggc cttctttcac ttcggagagg
     4021 cagccgacac caaggatggc gcgtgggtgg tattttttca ccattcgctt gatgaaggaa
     4081 gagccgggaa cgatgaacac cctgtagccc atcgcttcaa gtatctttct ccagaaacca
     4141 atggtgcact cgccacaact tctacacttc aggccctctg gagagaggtg ggccgggcac
     4201 cgcgacgacc gaaggcactg gggaaggaag atcgcccgtt ccgtgaccgg gatctcggag
     4261 aaactcttct tgttcatggt gttgtgcagg ttgatgaaga acgtcaggac ctcgctgtct
     4321 tcaagcccga aaaggcggaa gagtgatttg gcaagtcctt caacgagcac cataccggcc
     4381 ttgagaaacc ggggaaagta aagcctcccg gtattgattg aatagatgga aagcgcgacg
     4441 aggatgaatg aaaagaagaa gatcccgata atgacaacga ttgtgatctc tccgatcagg
     4501 aatacgagac tctgccacat ggaagcatca attggcacag gcctgcatcc ccctaaaggc
     4561 ccctgtcctt tcgggcggcc tcgatctcat tccagtcgac cggcgggctg ataacaccga
     4621 tatccgtaat gaacgccgtg accagctgat ggggagtgag gtcaaatgca tagtttttca
     4681 cacctacgcc gtccgggacc gtgacagtgc tcccacaaaa ggcaacctcg ttcctgtctc
     4741 tctgttcgat ggtgacctcg cgggcgatgc ggtgtgggtc gaacgtggaa agcggcgcgg
     4801 caacatagaa cggtacgcca tggtggcttg cgcagaccgc gtgcatatag gtcccgatct
     4861 tgttgaagac tgcgtcgggg gtgacccgat cggcacctac gatcaccatg tcaatctctc
     4921 cccgtctcat gaggaatgcc gcagacgagt cggggttcac tgtcacgggg atgccgtccc
     4981 tctggagttc ccaggcggtg agacgggctc cctggagcag cggccgggtc tcgcaggcga
     5041 tcacctgcac ctctgctccc gactccaccg cggagcggac gacgccgagt gcagtccccc
     5101 actcgctgca tgcaagcgcc cctgcattgc agtgggtgag caccgagctt gccttgcctt
     5161 caggaaacag ggcg
//
LOCUS       DAOQVJ010000047         5131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_17912_length_5131_cov_3.813239, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000047 DAOQVJ010000000
VERSION     DAOQVJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17912_length_5131_cov_3.813239"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKL26_01740"
     CDS             complement(<1..747)
                     /locus_tag="PKL26_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82446.1"
                     /translation="MNVIPAMTQKSTSELTISSRTQVFLIVLVTILVSIGYVAGTMLG
                     LSAITPLLFYLPIILAAYWFPRRGVLFAVGVGIAEVFFVYLYRYPSLPDITFAITTAS
                     FYVLVAIAVVISSLSSGLRDREARYRAIFSGSEASIFLVQNGDSELRIEEVNPGGGTL
                     IGRHPDDLKGESLIVFWRDDLARDSLLQSMKDDGSVSQMESVMTRENGTTVPVLISGS
                     RLPGRMMVLTVIDISARKAHEEEIEARNTQL"
     gene            complement(832..2160)
                     /locus_tag="PKL26_01745"
     CDS             complement(832..2160)
                     /locus_tag="PKL26_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82447.1"
                     /translation="MISVLFIDDEPALLEITRLFLERSGEIRVETCRSALEALEILKT
                     RSFDAIISDYEMPMMDGIVLLKILRAEGDNTPFIIFTGKGREHVVVDALNNRADFYLS
                     KGQDPKAQFAELDRMIRQAINRRIAEESLRLADRRMVDIINHLPDATFATDREGVVVS
                     WNKAMEDLTGVPAREMIGKGNHEYAIPFYDSPRPLLVDLINKPEDEIRAHSYQNIRRD
                     GNSIIAENPTGLRNGKPAVFWSRATLITDRKGTVIGAIESIRDITEVRSAEGKQKDQA
                     AREKESGMFDRIFGKSTASWYKKGVDLYYNHGRFQDAVSCFDRVIEMDPTHVNAWTEK
                     GICLKELGRYDEALQCFNRALELNGKLPATYYAKGETLEKLGKETGNFSLFEQAIACF
                     DLVLQKEPDNVNAWNYRGVCLKELGRFEEARRSFDKAQTILRLHPQKGLR"
     gene            complement(2315..3985)
                     /gene="ade"
                     /locus_tag="PKL26_01750"
     CDS             complement(2315..3985)
                     /gene="ade"
                     /locus_tag="PKL26_01750"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNS82448.1"
                     /translation="MEDFPSPAMMRQALGKEPADALFKNACVYDPFTCGWIETDIAVA
                     SGKIVGLGSLYKARNTLNLKGRRVVPGLIDAHVHVESSLLVPSEYARVVAMHGTTSVV
                     ADPHEIANVCGKAGIEYMLREREGLPVDIFIMLPSCVPATPLDRGGAELTAEDLSEFI
                     GRDGVLGLGEVMNVPGVLERDEGVFAKLALCSIRDGHAPLLSGESLNAYILAGLQSDH
                     ECTGKEEAAEKLSRGMFIFAREGSTEKNLRELIPLASPCTASRIAFATDDRHADSLVE
                     EGHIDDCIRKSLEYGLELEIVLRMATLSAADRFGLHDRGALAPGRNADFCILLEDEPF
                     SVLKTCKSGKILELSIREKPRIIPSPMRARVPTHEDLAVKGEGTARVIGIVPHQILTE
                     HLAFDLEGAQIPDLDRDILKVVVCNRYREGRCGLGLVHGFGLKKGAIAGSIAHDAHNL
                     VAVGADDQAIREALGGIIRNGGGMAAAGPDSLELLPLDCGGLMSSLPAEEVAGLLKTL
                     NGKAVDLGAIPGAFMYLSFLSLTVIPHLRVTDRGMFDADVFRDVPLFR"
     gene            complement(4175..4405)
                     /locus_tag="PKL26_01755"
     CDS             complement(4175..4405)
                     /locus_tag="PKL26_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82449.1"
                     /translation="MNKLVYEEKKFSTVSDCITQALIYFVDNQNDVGQFKENLHDYLA
                     SEEGKDFMKKIMKDLLVDVLTTQQKIAAEERR"
     gene            4638..5111
                     /locus_tag="PKL26_01760"
     CDS             4638..5111
                     /locus_tag="PKL26_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82450.1"
                     /translation="MHEILFFGVIFGGIFSLFSSLILVHMGMLIDRFPVGDEEKRERS
                     IWSPREFTRYAVMIYWPMYFAGAAMLVPGTRIMTVAGIATLFGLILFLITALIFSGAT
                     YNLMRAQGKGSGSPMATGSMIRPESILRAFRLSVQGRKIPFFRVSQAARTAAAEE"
BASE COUNT         1115 a         1389 c         1380 g         1247 t
ORIGIN      
        1 gagctgcgtg ttccttgcct cgatctcctc ttcgtgtgct ttccttgcgg agatatcgat
       61 cacggtgaga accatcatcc gcccgggcag cctggaccca gagatgagca cggggacagt
      121 ggttccattc tccctggtca tcacactctc catctgcgat acggacccgt catctttcat
      181 ggactggagg agggaatctc tcgccaggtc gtctctccag aatacaatga ggctttcacc
      241 tttcaggtca tcggggtggc gaccgatgag agttccgccg ccggggttca cttcttctat
      301 tctcaactcc gaatcgccat tctggacaag gaagatcgag gcctctgaac cggagaaaat
      361 agccctgtaa cgcgcctccc tgtccctgag gccgcttgaa agggacgaga tcacaacggc
      421 gattgcaacg agcacgtaga aactggccgt ggtgatggca aaagtgatat ccgggagact
      481 cgggtaccgg taaaggtaaa caaagaaaac ctcagcgata cccacgccaa ccgcaaagag
      541 cactccacgc ctgggaaacc agtaggcggc cagtatgatt ggcaggtaaa agagaagcgg
      601 agtgatggcc gagaggccga gcatggtgcc agcgacgtac ccaatgctga cgaggatagt
      661 caccagcacg atgaggaata cctgtgtcct tgagcttatg gtcagttctg atgtgctctt
      721 ttgagtcatt gcaggtatca cgttcaccaa tctattgctc cgtttccttt taaatatgga
      781 tgcttggcaa ccggagagtg cgagtgaaaa gagaagagga aagagcgggt tttagcgaag
      841 gcccttctgg gggtgcaatc gcaatatggt ctgcgcttta tcaaaggagc gcctggcctc
      901 ttcgaacctg ccaagttctt tcaggcagac tccccggtag ttccaggcat tcacattgtc
      961 gggttctttc tgcagcacaa ggtcgaaaca tgcgatagcc tgttcaaaga gtgagaaatt
     1021 gcccgtctct tttccaagct tctcaagggt ctcacccttg gcataatagg ttgcgggaag
     1081 tttcccgttc aattccaggg cgcggttgaa acactgcagc gcctcatcgt accggccgag
     1141 ttcttttaag catatgccct tctccgtcca ggcattcacg tgagtgggat ccatctcgat
     1201 aacacggtca aagcagctga ccgcatcctg gaacctgccg tggttgtaat agaggtcgac
     1261 tcctttcttg taccagctcg cagtggattt cccgaagatg cggtcgaaca tgccgctctc
     1321 cttctcacgc gctgcctgat ccttctgttt gccctcggca cttcggactt cagtgatgtc
     1381 gcgtatggac tcgattgcac cgattaccgt ccccttccgg tcagtgatca gcgtggccct
     1441 tgaccagaag actgcaggtt tgccgttgcg caatcccgtg ggattctcgg caataatgga
     1501 attgccgtcc ctgcggatgt tctggtaaga gtgcgcccta atctcgtcct caggcttgtt
     1561 gatcaggtcg acgaggaggg gtcttgggct gtcgtaaaag gggatagcgt attcgtgatt
     1621 gccttttccg atcatctccc gcgcaggaac tccggtcaga tcttccatgg ccttgttcca
     1681 tgatacaacg accccctccc tgtccgtcgc aaatgtcgca tcagggagat ggttgatgat
     1741 gtcaaccatc cggcggtcgg cgaggcgaag tgattcctcg gcaattcttc ggttgatggc
     1801 ctgccggatc atcctgtcga gttcggcaaa ctgcgctttc gggtcctggc ccttcgagag
     1861 atagaagtct gcacggttgt tgagggcgtc tacgacgacg tgctctctgc ccttcccggt
     1921 aaagatgatg aatggagtgt tgtcaccttc ggcacggagt atcttgagga gcacgatccc
     1981 gtccatcatc ggcatctcgt agtcggagat gatggcatcg aatgacctgg ttttcaggat
     2041 ttcaagggcc tcgagggcag accggcaggt ctctaccctg atctcaccgg acctctcgag
     2101 gaaaagcctt gttatctcaa ggagggccgg ttcgtcatcg ataaaaagaa cggatatcat
     2161 gccttcccct gtgattgaca tccctaccta aaactccatt tccatttctt tttacttaat
     2221 tctttcattt atttcctcta ttcttaccac tatcgctact gcggggtgag tgaaaagtca
     2281 tattggaggt gagggagcaa ccgctgggct gctctcagcg gaagagcggg acatccctga
     2341 acacatctgc atcgaacatg cccctgtcgg tcactctcag gtgcggaatg accgtgagcg
     2401 aaaggaaaga gaggtacata aaagcgcctg gaatggcccc aagatccact gcctttccat
     2461 tcagcgtctt cagcagccct gcaacctcct cagcgggcag agaggacatc agcccgccgc
     2521 aatcaagggg gaggagttcg aggctgtcgg ggcctgcagc tgccatccct cctccattcc
     2581 tgatgatccc tcccaaggcc tcgcggattg cctggtcatc cgctcccacc gccaccaggt
     2641 tatgcgcatc atgtgcaatg ctccccgcga ttgctccttt cttgaggcca aacccatgga
     2701 cgagaccaag gccgcatctc ccctcccggt aacggttgca gaccaccacc ttcaggatgt
     2761 cccggtccag gtccgggatc tgtgctcctt ccagatcgaa cgccaggtgt tctgtcagta
     2821 tctggtgcgg gactatcccg attaccctgg ctgttccctc tcccttgact gccaaatctt
     2881 catgggtggg gacacgagcc ctcatcggcg aagggattat ccgtggtttc tcccggatag
     2941 atagttcgag tatcttcccg gatttacagg ttttcagtac agaaaacggt tcatcctcca
     3001 aaagaatgca gaagtctgcg ttcctcccgg gtgcgagtgc acccctgtcg tgaaggccga
     3061 aacgatccgc agctgagaga gtcgccatgc gaaggacaat ttcgagttca aggccgtatt
     3121 cgagcgactt ccgaatgcaa tcgtcgatat gcccttcttc aaccagcgaa tcggcatgcc
     3181 ggtcatcggt ggcaaacgct atccgggacg ccgtgcaggg ggatgcgaga gggatgagtt
     3241 cgcggaggtt cttttccgtt gacccttccc tggcgaagat gaacataccc cggctcaatt
     3301 tttccgcggc ctcctccttc cccgtacatt cgtggtcgct ctgcagccct gcaaggatgt
     3361 aggcattgag ggattctcca gagaggagcg gtgcatgtcc gtcccgaatg gagcagagcg
     3421 caagtttcgc gaaaacccct tcgtcccgct ccagtacgcc ggggacattc atcacttctc
     3481 caaggcccag cactccatcc cgcccgatga actccgagag gtcctccgca gtcagttcag
     3541 ctcctcccct gtcaaggggt gtggccggca cacaggaagg aagcatgatg aagatgtcta
     3601 ccggaagacc ttcccgctca cggagcatgt actcgattcc tgccttgccg cagacgtttg
     3661 cgatctcgtg cggatcggcc acgacgctcg tggtgccgtg cattgccacc acccgtgcat
     3721 actcggaggg gacaagaagg gaactttcga catgtacatg ggcgtcgata aggccgggga
     3781 ccacccttcg tcccttcagg ttgagagtat ttctggcctt gtaaagactg cccagtccta
     3841 caattttccc ggatgccact gcaatatccg tttcaatcca gccgcaggtg aacgggtcgt
     3901 atacgcacgc attcttgaac agcgcgtcgg ccggttcttt cccgagggcc tgtctcatca
     3961 ttgcaggaga ggggaagtct tccatgagaa aaggagtggt ccgggatggc acaagaacat
     4021 tcttctcaag agattgtgac ccagggacgc cgcatccgat gaatgtagaa attagtagag
     4081 aggggaaggg caggtcccgg gtgaatagaa gaggtaaatg agacagaatg agcgattgac
     4141 gggacaggga ggttcttaaa gaaatattta ggtattacct gcgctcttcg gcagcgatct
     4201 tctgctgggt ggtcagtaca tccaccagga ggtctttcat gatcttcttc atgaaatcct
     4261 ttccttcctc ggacgcgagg taatcatgga ggttctcttt gaactgtccc acatcgttct
     4321 gattgtcgac gaaatatatt aatgcttggg tgatacagtc agagacggtt gaaaactttt
     4381 tctcctcgta taccagttta ttcacttttt cgtagatatt tgggggcatt ttgtaagaaa
     4441 tggccacccg ctctccccgt ttgttgtttc ccctatccac cattcctaat gaaatggagt
     4501 tccttcgtat aaaaaagctg cgaaaaaatg tgtattacct ttgaaatacc aattgggcga
     4561 cactttacct atgtattaca taaagaatac tttaaatagg aacgcctgcc atggtgatat
     4621 agtggaggca gacaatcatg cacgagatac tgttctttgg cgtaatcttc ggggggatat
     4681 tctccctttt ctccagtctc atactggtgc acatggggat gctgatcgac aggttcccgg
     4741 ttggggacga ggagaagagg gaacggtcta tttggagtcc aagggagttt acgcggtatg
     4801 cagtgatgat ttactggcca atgtattttg ccggtgccgc gatgctcgtc ccaggaaccc
     4861 gtattatgac tgttgcgggc attgccaccc tgttcgggct tatcctcttt ttgatcaccg
     4921 cactgatatt ttcgggggcg acctacaacc tgatgagagc tcaagggaaa gggagcggga
     4981 gcccaatggc caccggatcc atgataaggc cggagagcat cctgcgcgcc ttccgtctgt
     5041 ctgtccaggg gaggaagatc cctttctttc gggtatccca ggcagcgcga acggcggctg
     5101 ctgaggaata gggcgtcctt cctctctctt t
//
LOCUS       DAOQVJ010000048         5096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_18085_length_5096_cov_5.187066, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000048 DAOQVJ010000000
VERSION     DAOQVJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18085_length_5096_cov_5.187066"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKL26_01765"
     CDS             complement(<1..434)
                     /locus_tag="PKL26_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNS82451.1"
                     /translation="MLSEYDVISVDADGHGPRRVQVCREERVHLEVNGSHIASLTVSP
                     RELAEFACGYCVTEGIVPGIFAISEVEVKRDRIHVAAPAIEREILARPKEIRTSGCVG
                     VIQDEHTMRVPLPIAARVSRRVIYSAMECLNDHAVLWRETGGT"
     gene            650..1486
                     /gene="modA"
                     /locus_tag="PKL26_01770"
     CDS             650..1486
                     /gene="modA"
                     /locus_tag="PKL26_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS82452.1"
                     /translation="MKKNLFRVTPVTLGTLVCILMFLGAVTPGCITSKETTPTPGVSQ
                     AGILVYSGAGLKSAMTDIGQEFTRKYGIEVQYNYAGSGVHISQMNLTRKGDVFITGST
                     VEYNNAKNQGLVGPYQLVAYHVPVIAVQKGNEKNITMLQDFARPGLKIALGDVNATAI
                     GKAGAKMFKKLNITDAVEENVVTRTPTINELTILMTAGQADASILTLDLINDKTMEAI
                     QIPITVNEVMIIPVGITTFSENQDNARKFADFVASDEGKAIFVSHGFPKYPDPAYVDV
                     KP"
     gene            1517..2014
                     /gene="tsaA"
                     /locus_tag="PKL26_01775"
     CDS             1517..2014
                     /gene="tsaA"
                     /locus_tag="PKL26_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNS82453.1"
                     /translation="MLIEYAQIGVAHSPFRNAGAPPVHSTFMQAKGTIEIFPEYREGL
                     GSLEGFSHLIVIYHFDRAERRALKEPPLVDGETPHGIFATRHINRPNPIGISYVQLEK
                     ITDGILIVKGIDLLDGTPVLDIKPYIPAFDSIPDAATGWVTPQHIEKIRNVSLLATRD
                     NDTMQ"
     gene            2042..2815
                     /locus_tag="PKL26_01780"
     CDS             2042..2815
                     /locus_tag="PKL26_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82454.1"
                     /translation="MKRFSLILTYLVLVAISAVLLGLVTYSPLPDLVASFASPEIQFA
                     IVLSLVTSFASTAICIVIAIPVAYALARFDFFGKRIANLALTLPLTLPPLVAGIALLL
                     FFGTTPWGKALEQAGFMVIFTPLGIIVAEVFVNIPYMIRILRSAFSTINPRYEYVAKT
                     LGCTDTGAFFRVTLPMARSGLLAGTVITWSKAMGEFGAVLMVAGATTMRTETLPIALY
                     LNISTGDLDLAVAAATILILISLVTLCAVEYFDKGVNVF"
     gene            2829..3890
                     /locus_tag="PKL26_01785"
     CDS             2829..3890
                     /locus_tag="PKL26_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82455.1"
                     /translation="MLRIDSVSITLGEFSVRDVSLEIRPNEYFIILGPTGAGKTVLLE
                     TIAGIHTPGSGKIFLGNQEITSIEPRLRNIGMVYQDFMLFPHLTVRENISFGLKQRKL
                     PVDEQSTMVDEICALLEIPHLVGRYPGSLSGGEQQRVALARALVLKPEVLLLDEPMSA
                     LDRRTRERMRTELSRIRKLTGTTIVQITHHFDDVFAIADRIAIMREGRIVQVGETADV
                     FLHPADTFVAEFLGIGNIIRGTSSRAGYITVITPDTGPVFYASSRVSGDVVATLHAED
                     VIVSVAPFASSARNCLYGTVSEILAFGSTIRVIIDVGFPLTALLTRESCHELQIEPGS
                     RVYATFKATAVHVIPVNPE"
     gene            complement(4005..4757)
                     /locus_tag="PKL26_01790"
     CDS             complement(4005..4757)
                     /locus_tag="PKL26_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNS82456.1"
                     /translation="MSDFHILDDLLATLPEDPVPVRSVLVGVHWTAVCSRHCGLATTI
                     IANKPHDHATFVRNVGNLHQKTAQELAEYSRSKNRLEASIGVAAINSLLFMNQKDAVE
                     MNASEVLIKKGAGKNVALVGHFPFIPQVRRRVGNLWVIEQQPAEGDYPANTAADLIPQ
                     ADIVALTGSALINHTLEDLLSLCRPDALVMVLGPSTPLSEVMFDYGVSIIAGSQIIDE
                     AAVMRTVGQGATFQQVEGVRKLMYARRGASLV"
BASE COUNT         1222 a         1396 c         1292 g         1186 t
ORIGIN      
        1 gtaccacccg tctcccgcca gagcaccgcg tgatcattga ggcattccat tgcggaataa
       61 ataaccctcc tggacacccg ggcggcgatg ggaaggggca ccctcatagt gtgttcgtcc
      121 tggatgaccc ccacacagcc ggaggtccgg atctccttcg gccgggcaag aatttccctc
      181 tcgatggccg gggcagcaac gtggatccta tccctcttca cctccacctc gctgatggca
      241 aaaattccag gaacgatccc ttcagtcaca cagtagccac atgcgaattc tgcgagttcg
      301 cggggactca ccgtcaggga tgcgatatga cttccattca cctcaaggtg cacccgctct
      361 tccctgcaga cctgcaccct gcggggtcca tgaccgtccg catcaacgga gatcacatcg
      421 tattcgctaa gcatcggccc ggaatcgcat tcattctcaa atatcttcaa tacgaacgac
      481 cctcctcttt acatgcaatg aatcagaatg gtgcgcgccc attataattc tctttaagtt
      541 aagtactttc ttcgcaagat acacttttat aagttaacaa tggtaaattg ataaaaattg
      601 gttttcgaat gaaataatta attataattg atttggtgac tttttacgca tgaaaaagaa
      661 tctgtttcgc gtaacgccgg tcaccctcgg aacccttgta tgcattctga tgttccttgg
      721 tgcagtgacg ccgggatgta tcacgtccaa ggagaccacc cccactcctg gagtttcgca
      781 ggcgggcatc cttgtatatt ccggggcagg acttaaatct gcgatgactg atattggcca
      841 ggaattcacc cggaaatacg gtattgaggt ccagtataat tatgcaggaa gcggtgttca
      901 catcagccag atgaacctta caagaaaagg tgatgtattt atcacggggt ccacggttga
      961 gtataataac gcgaagaacc agggtcttgt gggcccatat cagctggttg cctatcatgt
     1021 tcccgtaatt gccgtgcaga aaggaaacga aaagaacatc acgatgctcc aggactttgc
     1081 caggccgggc ctcaagattg ccctcggtga cgtgaatgca accgccattg ggaaagcagg
     1141 agcgaagatg ttcaagaaac tcaatatcac cgatgcagtt gaggagaatg tcgtgaccag
     1201 gaccccgacc atcaatgaac tgaccattct tatgaccgcc ggccaggcag acgcttcgat
     1261 cctcactctt gacctgataa acgacaagac aatggaggca atacagattc ccataaccgt
     1321 gaatgaagtg atgatcatcc ccgtcggcat cacgaccttt tcggaaaacc aggataatgc
     1381 caggaaattt gcagatttcg tggcatccga tgaggggaag gcgatcttcg tttcgcacgg
     1441 ttttccgaaa tatcctgatc cggcatatgt ggatgtgaag ccataaagag ggaagaagac
     1501 tttggtgaat gtatccatgt tgatagaata cgcacaaatc ggtgttgccc attccccttt
     1561 ccgcaatgca ggagcacccc cggtgcacag cacgttcatg caggcgaaag ggaccatcga
     1621 gatcttcccc gaataccgtg aagggctcgg gagcctggag ggcttctccc acctcatcgt
     1681 catctatcat tttgaccgtg cggagcggcg ggctctgaaa gagcctccgc ttgtagatgg
     1741 ggagacaccc catggaattt ttgccacccg ccatatcaac cgcccgaacc caatcgggat
     1801 ctcctacgtg caactggaaa agattactga cggaatactc atagtcaagg gtatcgacct
     1861 ccttgatggg acgcctgttc tggatatcaa gccctatatc cctgcattcg acagtattcc
     1921 cgatgcagcc accgggtggg taacccccca gcatatcgag aagatccgaa acgtaagtct
     1981 ccttgccaca agggataatg acaccatgca gtaacagcag gacgcaaccg ggataccgcc
     2041 gatgaagaga ttctcgctca tactcaccta cctggtcctg gtcgccatca gcgcagtcct
     2101 ccttgggctt gtcacatatt ctccgcttcc cgatctcgtg gccagttttg cgagtccgga
     2161 gatacagttt gcgatcgtcc tcagtctcgt cacgagcttt gcatcaacgg ccatttgcat
     2221 cgtcatcgca attcctgtcg cgtatgctct ggcccgattc gacttcttcg ggaaaaggat
     2281 cgcaaacctc gccctcacgc tccccctcac cctccctccg ctggttgcag gaattgccct
     2341 cctgctcttc tttggaacga cgccgtgggg aaaagctctt gagcaggccg gattcatggt
     2401 cattttcact ccgcttggta tcatcgtggc agaagtattc gtgaacatcc cctacatgat
     2461 ccggatcctg cggtctgcgt tctccacgat caatccccgg tacgaatatg ttgcaaagac
     2521 cctcggctgc acggacaccg gggcgttctt ccgtgtcacc ctgccgatgg cccgatcagg
     2581 cctccttgcc gggaccgtca tcacctggtc aaaggccatg ggggagttcg gtgcagtcct
     2641 gatggttgcc ggtgcaacga caatgcggac agagacgctg ccgatagccc tctacctcaa
     2701 tatctcaaca ggggacctgg atctcgcagt tgcagccgcg accatcctga tcctgatctc
     2761 gcttgtgacc ctgtgtgcgg tggaatattt cgacaaagga gtcaatgtct tttaggaggt
     2821 gaggggtaat gctgcgcatc gattcggtct caatcaccct cggcgagttc tctgttcgtg
     2881 atgtgtcact cgagatacga cccaatgaat actttatcat tctcggaccg accggtgcgg
     2941 gtaaaacagt gctgcttgag acaatcgccg gaattcatac ccctggctca gggaagatct
     3001 tccttggcaa ccaggagatc acctccatag aaccgcgttt gcggaatata gggatggtct
     3061 accaggactt catgctcttt ccccatctca ccgttcggga gaatatctcg tttgggttga
     3121 aacagagaaa acttcctgtg gatgagcaat ccacgatggt ggatgagatt tgcgccttgc
     3181 tcgagatccc gcacctcgtc gggaggtacc cgggttcgct ttcaggaggg gagcaacagc
     3241 gtgtcgccct tgcccgtgcc ctggtactca agccggaggt acttcttctc gacgagccca
     3301 tgagtgcgct cgatcgccgc acgagagagc ggatgcggac tgagctctcc cgtatccgga
     3361 agctcactgg gacgacgatt gtccagatca cccatcattt cgacgatgtc tttgcgattg
     3421 cagacagaat agcaatcatg cgggaggggc ggatcgtcca ggtcggtgag actgcagatg
     3481 tattcctgca tcctgccgac acgttcgtcg cagaattcct cggcattggc aacataatcc
     3541 ggggcacgtc atcacgggca ggatatatta ccgtgataac tcccgatacc ggtccggtat
     3601 tctatgcctc ttcaagagtt tcaggcgatg tggtcgccac tctccatgcc gaggatgtga
     3661 tcgtctcggt ggccccgttc gcttccagtg cccgcaactg cctgtatgga accgtttcag
     3721 agattcttgc gtttggaagc acgatacggg ttatcatcga cgtgggattc ccgctcacgg
     3781 cgctccttac cagggagagc tgccacgaac tgcaaataga gccgggaagc agggtgtacg
     3841 caaccttcaa ggcaaccgca gtacatgtga ttccggttaa tccggaataa gtaaatcagc
     3901 atgtaccaat aaaaacgaaa aaaccgcatc ccttaccacg acagcattga tgcgttgcta
     3961 tcaaatggtt gacggatgta aatgtccccc gccagaaaaa tagtctatac cagggatgct
     4021 cccctgcggg catacatcag ttttcgcaca ccctcgacct gctggaaagt cgcaccctgt
     4081 ccgaccgtcc gcatcaccgc ggcctcgtcg atgatctgtg aaccggcaat gatagacacc
     4141 ccgtaatcga acattacttc tgagaggggc gtgctcggcc cgagaaccat cacaagagcg
     4201 tccgggcggc atagggagag aagatcctcc agggtatggt ttatcagcgc acttccggtg
     4261 agtgcgacga tatctgcctg cgggatgaga tccgcagcgg tgttcgcggg ataatcacct
     4321 tcagcaggct gctgctcgat cacccagaga tttcctacgc gccttcgaac ctggggtata
     4381 aacggaaaat gccccacgag ggccacgttt tttccggcac ccttctttat cagcacctcg
     4441 gatgcgttca tctcaactgc gtccttttgg ttcataaaaa gaagggagtt gattgccgca
     4501 acgccgatgc ttgcttccag gcggtttttc gagcgagaat attcggccag ctcctgtgca
     4561 gtcttctggt gcagattccc gacgttgcgg acaaaagttg catggtcatg gggtttattc
     4621 gcgatgatgg tcgtggcaag accgcaatgc cggctgcata ctgcagtcca gtgcaccccg
     4681 acgagaaccg aacgaaccgg gactggatcc tccgggagcg tcgcgagcag atcgtcaaga
     4741 atgtgaaagt cgctcatggt attacctcac tcctctctcc ggcaccagga agaaatggtt
     4801 gcggcatgag gaagcatgtt acaggatatt ggaattgatt acataagagg gtaaaggtaa
     4861 ttacattcac tcagtctatc ttggtatcaa gactcacaga cggattacag agaactccaa
     4921 aatcgcactt tacaaatgac ctgtcatgtc ggtgcctgta aatcacgcaa cagactgccc
     4981 catgtgttca ttagatggcc gggccttgaa gagtactcat tgtaagaatt aaagagatat
     5041 acgtactttt ctctaaaatt aaatacaaga agtcaacaca tggatgagag aaccgc
//
LOCUS       DAOQVJ010000049         5003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_18585_length_5003_cov_4.590340, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000049 DAOQVJ010000000
VERSION     DAOQVJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18585_length_5003_cov_4.590340"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKL26_01795"
     CDS             complement(<1..270)
                     /locus_tag="PKL26_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HNS82457.1"
                     /translation="MKKKFGGFIPLLVAMAMILSYSIAPIRDGVGAALDLVLGPLAGM
                     MPFYILIILLSAATGLYSSIIQKYTIDYERMTDVQERMKVFQREFR"
     gene            complement(277..849)
                     /locus_tag="PKL26_01800"
     CDS             complement(277..849)
                     /locus_tag="PKL26_01800"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNS82458.1"
                     /translation="MTGRRVIVTGVPGVGKTTVVTGALKVLEGEGITYRSLNFGTYMF
                     ETAQKEGFAKDRDEMRRLPGDVQKKLQQSAARAMAKESGNILVDTHASVRTPKGLLAG
                     LPEWVLRELMPDTIILVETDEDQILMRRLSDETRTRDMEGSRAIGDHQQFNRAIAASY
                     SMLTGCTIKYITNADFLLDRAIEEMAAVLR"
     gene            complement(852..2285)
                     /gene="secY"
                     /locus_tag="PKL26_01805"
     CDS             complement(852..2285)
                     /gene="secY"
                     /locus_tag="PKL26_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNS82459.1"
                     /translation="MGELLDRMEPLLAAMPTVKGPEGHVHFKNKLVWTAGILILYFVL
                     TNIPLFGLDPGSQDIFFYWRALLAGASGSLVQLGIGPIVTASIVLQLLKGADLIHIDT
                     SEMKGQIQYMGLQKLLIFVMIIIEAAPNLVGGFLMPDPVIADAFFGGNLFLVSFIIFL
                     QVCIGGVLIVFMDEVVTKWGIGSGVGLFIIAGISQAIINGFINWAPVSDQYPVGFFPR
                     IAAVIMDGANFLQYMGTDMIAFITTIAIFLIIVYVESTRIEIPLAHAQVRGARARFPV
                     KLIYASVLPMILVRVLQANIQMIGLFLNNVGITIFGTFQGATPVSGLMWYLAPINGPS
                     DWMWWLYDLGHAPWEVLTRMGIDILVVVIGGAIFALFWIKTAGMDSKDVARQIQHSGM
                     SIPGYRRNPQVLEKYLDRYIPRVTVIGGVFIGVLSVFANLFGVIGAVSGTGLLLTVSI
                     TYRLYEEIASQQIMEMYPFMRTFFGKE"
     gene            complement(2306..2731)
                     /locus_tag="PKL26_01810"
     CDS             complement(2306..2731)
                     /locus_tag="PKL26_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HNS82460.1"
                     /translation="MPVNKRSKFRGSRTCGGGTHKNRRGAGNRSGRGRAGHRDHRFSH
                     FLVYGEVHNGKHGFVNQTSAAANALSVGRLDEMVDELIAAGMASREGDAVVILADQIG
                     IDKILGNGKVTRKMHVSARAFSGQAKQKIEAMGGQALVI"
     gene            complement(2736..3197)
                     /locus_tag="PKL26_01815"
     CDS             complement(2736..3197)
                     /locus_tag="PKL26_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNS82461.1"
                     /translation="MYAIVQVRGVVNTRRDIKDTLKMLRLHHINHCVLVPDTPEYLGM
                     IRKVKDFVAYGEVDDETVETVLRTRGRVEGNRPLTDEYVKENSTYPGIRDFARALASG
                     EARLSDIPGVKPVLRLHPPRKGYRTIKRTYPQGGALGYYGREINELLYKMR"
     gene            complement(3197..3814)
                     /locus_tag="PKL26_01820"
     CDS             complement(3197..3814)
                     /locus_tag="PKL26_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNS82462.1"
                     /translation="MAYEKEEWVPLTGLGKMVVSGELTSIDQVLESGRPIKEPGIVDA
                     FLPDLEDEVLDITMVQRMTDSGRRVKFRAVVAVGNRNGYIGFGQGKDSQVGEAIKKAI
                     VDAKTNLIKVRRGCGSWECGCDTTHSIPMMVEGAAGSVRVTLKPAPQGIGLVTGDISK
                     KVLELAGIRDVWTFSRGQTRTTINFAKATFNALKATNMIRTGGQE"
     gene            complement(3816..4343)
                     /locus_tag="PKL26_01825"
     CDS             complement(3816..4343)
                     /locus_tag="PKL26_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNS82463.1"
                     /translation="MATGPRYFVPFRRRKEGKTNYYARAKLVVSDRPRMVVRRTNRHI
                     IIQLVTAEMDGDRTLVSANSGELSGFGFTGSTSSTPAAYLTGALFAVKALNADFESAI
                     LDIGLHRATRGARIFAALKGAVTAGLDVPHGEEILPDDDRVKGAHIAAYAPDRAGDLV
                     QNVEQTMDAIMKELE"
     gene            complement(4345..4797)
                     /locus_tag="PKL26_01830"
     CDS             complement(4345..4797)
                     /locus_tag="PKL26_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HNS82464.1"
                     /translation="MTDLSTQKRVSASIMKCGVHRVWFDPERTSDIQNAISREDLRTL
                     IDEDAIRALQPRGNSRGRAREKMAKRSYGHCKGPGRRKGAAGARSPRKREWIKKIRAI
                     RKTLADLRDTGEINPHLYRTLYRQAAGGQFRSVAHLKAHLALISGRMK"
     gene            complement(4790..>5003)
                     /locus_tag="PKL26_01835"
     CDS             complement(4790..>5003)
                     /locus_tag="PKL26_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="eL32 family ribosomal protein"
                     /protein_id="HNS82465.1"
                     /translation="VHTPDEIEGLDPALDAVRIAAAVGARKRGFIQEKAMAAGLKVLN
                     PKDLSKKKGAPVEEEPEADEEVEEND"
BASE COUNT          923 a         1563 c         1422 g         1095 t
ORIGIN      
        1 gcggaactcc cgctggaaga ccttcatcct ctcctgcaca tcggtcatcc gttcgtaatc
       61 gatcgtatac ttctggatga tcgaggaata gaggccggtg gccgcggaga ggaggataat
      121 caggatgtag aacggcatca tccctgcaag gggtcccagg acaagatcga gcgctgctcc
      181 cacgccatcg cgaatggggg cgatgctgta ggagaggatc attgccatgg ccaccagcag
      241 gggaataaag cccccgaact tcttcttcat cccggatcac ctgaggactg ccgccatctc
      301 ttcaattgca cggtcgagca ggaaatccgc attggtgatg tacttgatag tgcacccggt
      361 cagcatggaa taggacgcgg caatggcccg gttgaactgc tggtggtctc caatcgctct
      421 cgaaccctcc atgtcccttg tccgggtctc atccgagagc cggcgcatca gaatctggtc
      481 ttcgtcggtc tcgacgagaa tgatggtgtc cggcatcagc tccctgagca cccattcggg
      541 cagacctgcc agtaacccct ttggtgtcct gacagatgcg tgggtgtcaa cgaggatatt
      601 tccgctctcc tttgccatgg cgcgggcagc actctgctga agcttcttct gcacgtcccc
      661 ggggagccgg cgcatctcgt cacggtcctt cgcgaatccc tctttctggg cggtctcgaa
      721 catgtaggtc ccgaaattga gggatcggta ggtgatcccc tccccttcca ggactttcag
      781 cgcaccggtg acgacggtgg tcttgccaac ccccgggaca ccggtcacga tcactctcct
      841 tccagtcatt ctcactcctt cccgaaaaat gtccgcatga aggggtacat ctccatgatc
      901 tgctggcttg cgatctcctc gtagagccgg taggtgatgg acaccgtgag cagaagcccc
      961 gtaccactca ccgccccgat cacaccgaag aggttggcga agaccgagag gaccccgata
     1021 aacaccccgc cgatgacagt gaccctgggg atgtagcggt caaggtactt ctccagcacc
     1081 tgggggttcc tccggtatcc cgggattgac atccccgagt gctggatctg acgggccacg
     1141 tcctttgagt ccattcccgc ggtcttgatc cagaacaggg cgaagattgc gccaccgatc
     1201 accacgacca ggatatcgat ccccatcctg gtgagcacct cccagggagc gtgcccgagg
     1261 tcatacagcc accacatcca gtccgacgga ccgttgattg gggcgagata ccacatcagg
     1321 ccgctcacgg gagtggcgcc ctggaacgtg ccaaagatgg tgatcccgac attattgagg
     1381 aagagcccga tcatctggat gttcgcctgc agcacccgga cgagaatcat cggcagcacg
     1441 ctcgcgtaga tcagttttac cgggaacctc gcccttgccc cccgtacctg tgcatgtgcg
     1501 agcgggatct cgatcctcgt ggactccaca tacacgatga tcaggaagat ggcgatggtg
     1561 gtgatgaacg caatcatgtc agtgcccatg tactggagga aattggcacc gtccatgatg
     1621 acagcggcta tccgggggaa gaaccctaca gggtattgat cgcttacagg cgcccagttt
     1681 atgaacccgt tgataatggc ctgggatatc ccggcaatga tgaagagccc gacccctgat
     1741 cctatgcccc acttggtgac cacctcgtcc atgaatacga tcaacacccc tcctatacac
     1801 acctgcagga agatgatgaa cgagacgagg aacaggtttc caccgaagaa tgcatctgcg
     1861 atcacgggat ccggcatcag gaaaccgccc actaggttcg gggcggcctc gatgatgatc
     1921 atcacgaaga tgagaagctt ttgcaggccc atgtactgga tctggccttt catctcgctc
     1981 gtgtcgatgt ggatcaggtc ggcccctttc agcagctgca gcacgatgga tgcggtcacg
     2041 atgggaccga taccaagctg cacgagagac ccgctggcac ctgcaaggag ggcgcgccag
     2101 tagaagaaga tatcctggga acccgggtcg aggccgaaga gcgggatgtt ggtaagcaca
     2161 aaatacagaa tcaggatccc tgcagtccag accagcttgt ttttgaagtg gacgtggcct
     2221 tcaggccctt ttacagtggg catcgctgcg agcagcggtt ccattcggtc cagaagttct
     2281 cccatttttt caccacaaaa agggattaga taaccagggc ctggccaccc atcgcctcta
     2341 tcttctgttt tgcctgtcct gagaatgcac gcgcggacac gtgcatcttc cgtgtgactt
     2401 ttccgttgcc aagtatctta tcgataccga tctgatccgc gaggatgacc actgcgtcac
     2461 cctcgcggga ggccatgccg gccgcaatga gctcgtcgac catctcgtcg agcctgccca
     2521 cagagagggc atttgccgct gccgaggtct ggttgacaaa accatgcttg ccgttgtgca
     2581 cttccccata caccaggaaa tgggagaacc ggtggtcgcg gtgacctgcg cggcccctgc
     2641 cgctccggtt ccctgcacct ctcctgttct tgtgggtgcc cccgccgcat gtccttgaac
     2701 cacggaattt ggatcgcttg ttcactggca ttgcttcacc tcatcttgta caggagctcg
     2761 ttgatctccc tgccgtaata tccaagggcc ccgccctgcg ggtatgtcct cttgatggtg
     2821 cggtatccct tccggggagg gtggagcctg agtaccggtt taacgccggg aatatccgac
     2881 aagcgggctt cccctgatgc aagggccctt gcaaagtccc tgatgccagg gtaggtggaa
     2941 ttttccttca cgtactcgtc ggtgaggggc ctgttccctt caaccctgcc cctggtgcga
     3001 aggacagtct cgacggtctc gtcatccacc tcgccatagg ccacgaaatc cttgaccttc
     3061 cttatcatcc cgaggtactc cggggtatcc gggaccagca cgcagtggtt gatgtggtgc
     3121 aggcggagca tcttcaaggt atctttgatg tcccgccggg tattcaccac cccgcggacc
     3181 tgcacgattg cgtacattac tcctggcctc ccgtgcgaat catgttggtc gccttcagcg
     3241 cgttgaaggt cgccttggca aagttaatcg tagtcctggt ctggccgcgg gagaacgtcc
     3301 atacatcacg gatgcccgcc agttccagca ctttcttgct gatatctcct gtcacgagac
     3361 cgatgccctg gggcgccggc ttgagggtga cccgcacact gcctgccgca ccttccacca
     3421 tcatggggat ggagtgggtg gtatcgcagc cgcattccca gctgccgcaa ccccttcgca
     3481 cttttatcag gttggtcttg gcatccacga tcgctttctt gatcgcctct cccacctggg
     3541 agtctttgcc ctgcccgaac cctatgtagc cattcctgtt cccgaccgcc accacggcgc
     3601 ggaacttcac ccggcggccg gagtcggtca tccgctgcac catggtgatg tcaaggacct
     3661 cgtcctcgag gtcagggagg aaggcatcga caatgccggg ctccttgatg gggcggccgc
     3721 tctccagcac ctggtcgatg ctggtcagct cgcccgagac caccatcttc ccaaggccgg
     3781 tcagcgggac ccattcctct ttctcatatg ccatattact ccagctcctt catgatggcg
     3841 tccattgtct gttccacgtt ctgcacgagg tcccctgcac ggtccggcgc ataggccgcg
     3901 atgtgcgcac ctttcacccg gtcatcgtcg gggagaatct cctcgccgtg cgggacgtcg
     3961 agtcctgcgg tcaccgcccc tttcagtgcg gcgaatatgc gggctccccg cgttgcacgg
     4021 tgcaggccga tatcgaggat tgcggactca aaatcggcat tcagggcttt gaccgcaaag
     4081 agcgccccgg tgaggtatgc ggccggggtg cttgaggtcg accccgtgaa tccaaaaccg
     4141 gagagctcgc ccgagttggc ggagaccagt gtacgatcgc cgtccatctc tgcagtgacc
     4201 agctggatga tgatatgcct gttcgtgcgg cggaccacca tccggggccg gtcggatacg
     4261 accagttttg ccctggcgta gtagttggtc ttgccctcct tcctcctgcg gaagggaacg
     4321 aagtagcgcg gtcctgttgc catgttactt catcctcccg cttatgagcg caaggtgggc
     4381 cttcaggtgc gcgacgctcc tgaactgccc cccggcggcc tgccggtaga gcgtgcggta
     4441 caggtgggga ttgatctccc cggtgtcgcg gagatccgcg agagtcttgc ggatcgcgcg
     4501 gatcttcttg atccactcgc gctttctggg gctcctggcc ccggccgccc ccttgcgccg
     4561 ccccgggccc ttgcagtgcc cgtatgaccg cttggccatc ttctcccttg ctcttcccct
     4621 gctgttgccc ctgggctgga gggcccggat tgcatcctcg tcgatgaggg tgcggaggtc
     4681 ttcgcgggag atcgcgttct ggatatcgga ggtccgctcg gggtcaaacc agacccggtg
     4741 gaccccgcac ttcatgatcg atgcggatac cctcttctgg gtggagagat cagtcatttt
     4801 cttccacctc ctcgtcggcc tcgggctcct cttccacggg tgcccccttc ttcttggaaa
     4861 ggtctttcgg gttgaggact ttcagccctg cagccatggc cttttcctgg atgaaacccc
     4921 tcttccttgc cccgaccgcg gctgcgatgc gaaccgcgtc gagcgcgggg tcgagtccct
     4981 cgatctcgtc aggtgtgtgg acc
//
LOCUS       DAOQVJ010000050         4991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_18641_length_4991_cov_4.209684, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000050 DAOQVJ010000000
VERSION     DAOQVJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18641_length_4991_cov_4.209684"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKL26_01840"
     CDS             <1..667
                     /locus_tag="PKL26_01840"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313748.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNS82466.1"
                     /translation="LAGNLPASQPCATILTMVEVEPDDHAFLHPTKFVGPVYSEDEKE
                     QVSREKGWTFRRDGNAWRRVVPSPLPRRIVEIQPIRWLLSHGAIVICAGGGGIPVIAA
                     PGGRRVLAGVEAVIDKDRASALLAEELKADLLVMATDVEGVFLDWNTPSARLIHSTNP
                     ARLRKHHFAAGSMGPKVEAACSFVDATGSRAAIGALSDIRGLVLGDAGTQVSAAGPGE
                     TEA"
     gene            670..1929
                     /locus_tag="PKL26_01845"
     CDS             670..1929
                     /locus_tag="PKL26_01845"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HNS82467.1"
                     /translation="MSEFGVYSEVGRLRKVLVHRPDLSARRLTPGNHQDLLFDDVLWV
                     ERAMEEHDAFVRVLEEEGVRVYRLASLLADVLAGKEARRWVIERAVHPMTVGRSACGS
                     VRACLMEMEPGALAEHLIGGLTRSELECIYLEGMTRSSLQVAASDPDSFILPPLPNTL
                     YTRDTSSWICGGFTLNPMFWPARRAEVVNTAAIYRFHPLFSNGGIRTWYSCLDHGNAA
                     PPASSATASMEGGDIMPIGRDTLLVGMGERTGGPMVEELARVLLMSGAVERIIVAQMS
                     HDRAHMHLDTVCTMLDESTVTVFPKVVEKIRAFSIRPGEGERIFEVTREEDFLGAVAD
                     ALGVGRLLVIPTGGDEYQAAREQWDDGNNVLAIAPGRVVAYDRNTCTNRNFEKAGIDV
                     IEIAGSELSRGRGGGHCLTCPLKRDPI"
     gene            2006..2755
                     /locus_tag="PKL26_01850"
     CDS             2006..2755
                     /locus_tag="PKL26_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNS82468.1"
                     /translation="MTPVLLPLFQKRIFDRRQELEAVREYPVEKLADIIGEVGFSCTY
                     CARCCTRAFNGHVLLLDEDAARIRSIDPTALEPQPEYDLCDQYGTFYVSGYTLRAMPG
                     ESGECHFLTDGRCRIYPDRPLICRVYPYMLHREPDEVGVVDWRQVSGLDQHGDYHCTV
                     PRDIAREIACEVKAFECAVISHEIAFLLYAGSYFSVHGLRHVRKRFDDGMRSLRTGTA
                     VRVKVFFGGTFESWVVRGGKCTPSDEKRQGV"
     gene            complement(2756..3286)
                     /locus_tag="PKL26_01855"
     CDS             complement(2756..3286)
                     /locus_tag="PKL26_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNS82469.1"
                     /translation="MATSHPDYFDARVFQPLRKADDEARLTWKERHLTQVNRMGLFDK
                     LKGSKAVELNPKSALVLSAITVIASDGVIDEAEMMDLAKIARGDRNAVDTAVKILQGI
                     TLPEAVDLVAKALNDQQRIATMAILTDLAMSDGVLAGNEQKILQLYMEKFGITETALK
                     PIIDAIAIKNDFSVFS"
     gene            complement(3267..4292)
                     /locus_tag="PKL26_01860"
     CDS             complement(3267..4292)
                     /locus_tag="PKL26_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNS82470.1"
                     /translation="MYTGIQLFRPYYILIVLACGVILLAGMKAASPILGPVLCAGFIS
                     ILFIVLKHWLEKKGFSPRLAVVAVILLYFTTLAGFVLAVGVSLLQLASQLPLYQEGLE
                     SLLPSATECGQVSFADLLTLLPSDAIDLLMTTGRGVIALLGDLLTAALIVVFTPLFIL
                     AESAGLTRKLQGVIVHLSGRMESAERFGRQMVEYLVVRTEVNLANGVVNGLFFACIGV
                     DFAILWGFLAFVLGYVVYVGFWLAVIPPMVLAWLEMGPAAALLVLIGAGIINTVAEYL
                     LFPHLAGYQLNLSPLVIFLSIFFWWLILGAFGVLLAVPLTMAVQAILETWDETRWLGE
                     LLGNESP"
     gene            4450..>4991
                     /locus_tag="PKL26_01865"
     CDS             4450..>4991
                     /locus_tag="PKL26_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNS82471.1"
                     /translation="MTDEVTSAALKVDDVMHLFPWWIVLLQGIVSLLLGIMFLAYPMG
                     TLFVMVTFLGAYWFVSGIFALVSLATDKTNMGVKVVLALLGIIAGILILAYPYYSTII
                     VPEIFVIMIAVWGVMMGCVSLFSSFKGGGIGAAVIGVLAIIFGCIILFNPLITVIYLP
                     FVLGIFGIIGGLAAIFFSFQV"
BASE COUNT         1047 a         1346 c         1562 g         1036 t
ORIGIN      
        1 gctggcgggc aacctgcccg catcgcaacc atgcgccacg atcctcacca tggtcgaggt
       61 cgagccggac gatcatgcgt ttctccaccc caccaaattc gtgggccctg tttacagcga
      121 ggatgagaag gagcaggtat cgcgggagaa gggctggacc tttcgaaggg acggaaatgc
      181 ctggcgaaga gtcgtgcctt caccccttcc ccggaggatc gtggagatcc agccgatcag
      241 gtggcttctt tcccatggtg cgattgtcat ctgcgcaggc ggagggggta ttcctgttat
      301 cgcggccccc ggcgggaggc gggtgctcgc aggagtcgag gcagtgattg acaaggaccg
      361 cgcaagtgcg ctgctggcag aggaactcaa agcggatctc ctcgtgatgg caactgacgt
      421 ggagggtgtg ttccttgact ggaatacccc ttcggcgcgc cttatccatt ccacgaaccc
      481 ggcaaggctc cggaagcatc actttgcggc gggatccatg ggcccgaagg tggaggcggc
      541 gtgcagtttt gttgacgcga cggggagccg ggcagcgatt ggggcgctct ccgatatcag
      601 gggactggtc cttggggatg cagggaccca ggtgtctgcg gcaggtccag gagagactga
      661 ggcataggca tgtcggaatt cggggtatat tcggaagtcg gaaggctgcg gaaggtgctc
      721 gtccacaggc cggacctgag cgcaaggagg ctcacaccgg ggaaccacca ggacctcctc
      781 tttgacgacg tcctctgggt ggagagggcg atggaggagc acgatgcctt tgtgcgggta
      841 ctcgaagagg agggggtccg ggtgtatcgt ctcgcctcac tccttgcgga cgtgctcgca
      901 ggcaaagagg cccggaggtg ggtgatcgag agggccgtac acccgatgac agtcgggcgt
      961 tccgcgtgcg ggtcggtgag ggcgtgcctc atggaaatgg agcccggcgc actggcagag
     1021 cacctcatcg ggggactcac acggagcgag cttgagtgta tctatctcga ggggatgacc
     1081 agatcgtcac tccaggtcgc ggcatcggac cccgactcct tcatcctccc cccgctcccg
     1141 aacaccctgt acacccgcga cacttcttcg tggatatgcg gggggttcac actgaacccc
     1201 atgttctggc cggcacgaag ggccgaggtg gttaacaccg cggcaatcta tcgcttccac
     1261 ccgctcttct caaatggcgg tatccggacg tggtactctt gtctcgatca cgggaatgcc
     1321 gcgcctcctg cgtcatccgc aacagcctcg atggaagggg gggatatcat gccaatcggc
     1381 agggatacgc tccttgtcgg gatgggcgag aggacgggag ggcccatggt cgaagagctt
     1441 gcccgggtgc ttcttatgtc aggcgcggtg gagaggatca tcgtagccca gatgagccac
     1501 gatcgggcgc acatgcacct tgacacggtc tgtaccatgc ttgacgagag tacggtcacg
     1561 gtattcccga aggtcgtcga aaaaatcagg gcattctcca tccgccccgg ggaaggggaa
     1621 aggatcttcg aggtgacccg cgaggaggac ttcctcgggg cagtagccga cgccctcgga
     1681 gtcgggcgtc tgctggtcat tcccaccggc ggcgatgagt accaggccgc acgggagcag
     1741 tgggacgacg gcaacaatgt ccttgcaatc gcgcccggcc gggtggtggc gtatgacagg
     1801 aatacctgca cgaaccggaa cttcgagaag gccgggatcg atgtcatcga gattgcggga
     1861 tcagagctct cgaggggacg cggaggcggg cactgcctga cctgccccct caagagagac
     1921 ccgatctgac tcctgaaatc actccccggc atctttgatc tcattgcttt ttcatcacgg
     1981 gtgacagaca cttctccaga gaacgatgac tcccgtgctt ctcccgctgt ttcagaagcg
     2041 gatttttgat cgacggcagg aactggaggc agtgagggag tacccggtgg agaagctcgc
     2101 tgatattata ggggaggtgg ggttctcatg cacctactgt gcaaggtgct gcacgagggc
     2161 attcaacggc cacgtcctcc tcctcgacga agacgcggcg aggatccgct cgatcgatcc
     2221 caccgccctc gagccgcagc cggagtatga tctctgcgat cagtacggga cgttctacgt
     2281 gtcaggatac actctcaggg caatgccggg tgagagtggg gaatgccatt ttctcacgga
     2341 cggccggtgc cggatctatc cagaccggcc cttgatatgc cgcgtatacc cctacatgct
     2401 ccaccgggag ccggatgagg tgggggtggt tgactggcgg caggtcagcg gccttgacca
     2461 gcacggcgat taccattgta ctgtcccgcg cgatattgcg agggagatcg cctgcgaggt
     2521 gaaggcattt gaatgcgcag tgatttcaca cgagattgcc ttccttttgt atgcgggcag
     2581 ttatttctca gtccacggcc tccgccatgt ccggaagcgg tttgatgacg gaatgcggtc
     2641 cctccgcacc gggaccgcgg tcagagtaaa ggtctttttc ggaggaacgt ttgaatcctg
     2701 ggtagtgcgg ggaggaaaat gcacgccgtc agatgaaaaa aggcaggggg tctgatcagg
     2761 agaagaccga gaaatcgttc ttgatcgcga tggcatcgat gatgggtttg agtgcggttt
     2821 ctgtgatccc gaacttttcc atgtacagct ggaggatctt ctgctcgttt cctgcgagga
     2881 caccgtcaga catggcgagg tccgtcagga tcgccatggt tgcaatcctc tgctgatcat
     2941 tgagcgcctt tgcgacgaga tcgactgcct cggggagggt gatgccctgg agtatcttga
     3001 ctgcagtatc cacggcattc ctgtcaccgc gtgcaatttt cgcaaggtcc atcatctcgg
     3061 cctcgtcgat cacgccatcg gaggcgatca ctgtgatcgc ggacagcacg agtgctgatt
     3121 tcgggttcag ttcaacggcc ttgctccctt ttaacttgtc aaagagtccc attctgttca
     3181 cctgcgtcag atgacgttcc ttccaggtaa gtcgtgcttc atcatctgcc tttcgcaggg
     3241 gctggaaaac ccgggcgtcg aagtaatcag ggtgactcgt tgccaagaag ttctccgagc
     3301 catcgcgtct cgtcccaggt ctcgaggatt gcctggactg ccatggtgag agggacggcg
     3361 agcagcaccc cgaatgcacc gaggatcagc caccagaaaa agatggagag gaatatgacg
     3421 agtggcgaga ggttgagctg ataccctgca aggtggggga agagcaggta ctccgcgacg
     3481 gtgttgatga ttcccgctcc gatgagcacc aggagagctg cggcgggtcc catctccagc
     3541 caggcgagca ccatcggggg aatgactgca agccagaacc ccacgtagac cacgtacccg
     3601 aggacaaacg cgagaaaccc ccacaggatg gcgaagtcca cgccgataca cgcaaaaaaa
     3661 aggccgttta cgacgccgtt tgcgaggttg acctcggttc ggaccacgag gtattccacc
     3721 atctgccttc cgaaccgttc agcactctcc attctccccg aaaggtgtac aatgaccccc
     3781 tgcaatttcc tggtcagccc tgccgactcg gccaggatga agagcggggt gaagacgacg
     3841 atgagtgcag cggtgaggag gtctccaagg agtgcgatga cacctcttcc ggttgtcatc
     3901 agcaggtcaa tcgcatcgct gggaagaagt gtgaggagat ccgcgaaaga gacctgcccg
     3961 cattccgttg cgcttggaag aagagactcg aggccctcct ggtagagcgg cagttgactg
     4021 gcaagctgca ggagggaaac cccgactgcc aggacgaatc ccgccagtgt tgtaaagtaa
     4081 aggaggatga ctgccacgac tgcaagacgg ggagagaacc ccttcttctc gagccagtgc
     4141 ttcagcacga tgaagaggat agagataaag cctgcacaga ggacagggcc aaggattgga
     4201 gaagcagcct tcattccggc gaggagtatc actccgcatg caaggacaat gagaatgtaa
     4261 taaggccgaa agagctgaat gcccgtgtac acaggggaaa aagacgcggt cgcgtgttat
     4321 caatcttgcc ccccgggatt caaaactcgc acatcaggaa tggcaaaggg ggtgagagtg
     4381 aggaaataag agagaaacgt tcatatactc ctcattgaga cgtaatctct gcgagagaag
     4441 tgtgaactca tgacagatga agtgacttca gctgcattga aggtggatga cgtgatgcac
     4501 ctctttccct ggtggatcgt cctcctccag ggtattgtat ccctccttct tggaataatg
     4561 ttccttgcct atcctatggg gaccctcttt gtaatggtga cgtttcttgg agcgtactgg
     4621 tttgtgagtg ggatctttgc cctcgtgagc cttgccaccg acaagaccaa tatgggagta
     4681 aaggtcgtgc ttgcattact cgggatcatt gcgggcattc tcatcctcgc ctacccctat
     4741 tacagcacca tcattgtccc ggagattttt gtcatcatga tcgcagtctg gggtgtcatg
     4801 atggggtgcg tgagcctctt ctcctccttc aaggggggcg gtatcggggc agcagtgatt
     4861 ggcgtgctcg caatcatctt cgggtgcatc atcctcttca acccgttgat cacggtcatt
     4921 taccttccgt ttgtgctggg catatttggt atcattggcg gacttgcagc gatattcttc
     4981 tcgtttcagg t
//
LOCUS       DAOQVJ010000051         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_18705_length_4980_cov_3.675736, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000051 DAOQVJ010000000
VERSION     DAOQVJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18705_length_4980_cov_3.675736"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKL26_01870"
     CDS             complement(<1..575)
                     /locus_tag="PKL26_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNS82472.1"
                     /translation="MSTLSEQILGAPAGDYVDCPVDRSYVHDGTGVLTLEAWRAMQAG
                     VLARPDRCYVLFDHIVPANNSVTAGLQQDLRTFAMEHGLNFTDVGEGICHQLMGEGIA
                     LPGEIIVGADSHTCTLGAFGAFATGVGATDMAAIWAGGGTWFRVPETIAIRLHGALSL
                     PAEAKDLALTYVGRLTMDGATYRALEFVGEGA"
     gene            complement(572..1702)
                     /locus_tag="PKL26_01875"
     CDS             complement(572..1702)
                     /locus_tag="PKL26_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HNS82473.1"
                     /translation="MRSWHIEICDVTLRDGEQTPGVSFTCEEKTRIAEVLDQIGVEVI
                     EAGFPATSPYEKRCVSAVASLGLDARICCLARARRADVDAAIEAGVDMVSIFMPTSEL
                     HIRHKFHAPRENVLDEALRMVDYTHDHGLQVRFAAEDASRTDLPFLMEVYRRGEEHGA
                     DLLSFADTVGALTPLQMYRTMRKIVRAVGKPLCAHCHNDMGMATANTLAAAEAGAFQL
                     HTTVNGIGERAGNAALEEVLVGLHMKGGVRRYDLSHLVPLSEMVVHYSGVGIDRLKPV
                     VGDHAFRHESGIHIAAILEDPETYEYFPPELVGTTRKFTLGKHTGKKALEHEVARLGY
                     HLPEKDLCQVLEMVKDRGEKKVDLTDETLIDLIKQVMGGPPL"
     gene            complement(1674..2963)
                     /locus_tag="PKL26_01880"
     CDS             complement(1674..2963)
                     /locus_tag="PKL26_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS82474.1"
                     /translation="MKGVEALAHAILDATDRRYTVPGYPVTELGPMTGAEMVINEKTG
                     LEYALGDSLSGRRAAVIVKNVGVNACVDPLLQATAQGLVGGVLLVAGDDPDAVGSQTS
                     QDTRIIGELAEIPVIEPDSGSAFRAVEAALQASEEFSRVAMIRVTPGFLEGEATDSPV
                     PRKDSTGRLSDRSYTMHGRVMASRTLFRKMQEWADASPLNAWCGEPAGVGPEPGRSRV
                     VTVFPPPARLAEFRQVREAGLTFTKDHCRHEILPEEEGRAPPQTLEERGYYRTFCRDC
                     PFKPVISLMQDLGMRPVCDAGCVVLAMNPPYCIGVASYGMGSSVAVAARSTGVALTGD
                     YAFLHSGINALMDVYAKGLSLLCVILDNRCAAMTGKQPSLPVERYLGWADPHVVDVQD
                     IEEIRHQLQHAARPGVIVIRGTCPEGQEHEIVAYRDM"
     gene            complement(2960..3919)
                     /locus_tag="PKL26_01885"
     CDS             complement(2960..3919)
                     /locus_tag="PKL26_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS82475.1"
                     /translation="MLKARLLEAGAARLEGEPADGYISASTAGPGARGPGSVFFSHGG
                     RRVRLAIDPESSIRVMHRGGGEAVLSVDGKEIAGSLEKIALHCPRQAYITVSSGCIFS
                     CRYCEVPGHPPRRKSIDEIVAMVEGVADRIDAISLTSGVMGSVEEEERYVLDVVQKLG
                     KFGIPIGVSIYPTNESAERLCALGVREVKFNVETATDALFEKMCRGLSRDDIWRALAQ
                     SVPLFGKNRVFSNLIIGLGETDEQVEECIQDLTRMGVIPVVRPLSPAAGCRGYRRPSK
                     ERILSINRIHAEALCEAGLDPGAALTMCVACTGCDLVPGRDDT"
     gene            complement(3913..4830)
                     /locus_tag="PKL26_01890"
     CDS             complement(3913..4830)
                     /locus_tag="PKL26_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HNS82476.1"
                     /translation="MRDPENAGQESLKDPYVIRYPEIVALSSRDGTQVELVEFFECVG
                     GAMWARLHYAKSPLVHSVRTVGGSTRYLLSPGQVDLGLEGSRFPAGICGVEVSRDEIA
                     VSYVGLGGGGVGASGCRSLAGGVLRSECDPSGGGKKAGATLWLPRRERVLIGVDDTDT
                     PEEGATWTLCHNIASAVQDADSRYLSHTIVQLFPVPYRTKNCVAVVCEFATRDPARLV
                     SSFQRLLERYTLSEKTGMAVFTGFDPAPLGEYGWAVKRGEVNRERLAALKGRDLEYLM
                     EGRGIIGAVAAIPFSTRFEEALQLCDGTC"
BASE COUNT          834 a         1711 c         1482 g          953 t
ORIGIN      
        1 gccccttctc ccacgaactc gagcgcacgg taggtcgccc cgtccatggt gagccttccc
       61 acgtaggtaa gggcgaggtc ttttgcctcc gcggggagag agagcgcccc gtggagcctt
      121 atcgcgattg tctccggcac gcggaaccac gtccctcccc cggcccagat ggccgccatg
      181 tccgttgcgc ccacgccggt ggcaaacgcc ccgaatgccc cgagggtgca ggtgtgcgag
      241 tccgccccca cgatgatctc gcctggaagg gcaatcccct cacccatcaa ctggtggcag
      301 atcccctccc cgacatcggt gaagttgagg ccatgctcca tggcaaaagt gcggaggtct
      361 tgctgcaatc ctgccgtgac cgagttgttt gcagggacga tgtggtcgaa gaggacataa
      421 cagcggtcgg gccttgccag gacccctgcc tgcattgccc tccatgcctc gagcgtgagc
      481 acccctgttc cgtcgtgcac gtaagatcgg tctactgggc agtccacgta gtcgcctgca
      541 ggcgctccga ggatttgctc cgaaagcgtg ctcacagtgg cggccccccc atcacctgct
      601 tgatgaggtc gatcagagtc tcgtcggtca ggtccacctt cttctccccc cggtccttga
      661 ccatctcgag cacctggcag agatcctttt ccggcagatg gtacccgagc ctggccacct
      721 cgtgctcgag cgccttcttg ccggtgtgct tgccgagggt gaacttccgc gtcgtcccga
      781 cgagttccgg cgggaagtac tcgtaggtct cgggatcttc gaggattgcg gcgatgtgga
      841 tgccgctctc gtgccggaag gcatgatcgc ccaccactgg cttcagccgg tcgatcccga
      901 cacctgaata atggacgacc atctccgaga gggggacgag gtgggagagg tcataacgcc
      961 tgactccgcc cttcatatga agtcccacga gcacctcctc gagcgccgca ttcccggcac
     1021 gctccccgat gccgttcacc gtggtgtgga gctggaacgc ccctgcctcc gcggcggcaa
     1081 gagtattggc cgtggccatt cccatgtcat tgtggcagtg ggcgcagagt ggctttccca
     1141 ccgccctgac tatcttccgc atggtccggt acatctggag gggggtgagg gcccccactg
     1201 tatctgcaaa tgagagcagg tctgcaccgt gctcctcgcc cctccggtac acttccatga
     1261 ggaacggcag gtctgtcctg gacgcgtcct cggctgcgaa cctgacctgg aggccatggt
     1321 catgggtata atccaccatc cgaagtgcct catcgagcac gttctcccgt ggggcatgga
     1381 acttgtgccg gatgtgcagt tccgacgtgg gcatgaagat gctcaccatg tcgacgccgg
     1441 cctcgatggc cgcgtccaca tccgctctcc tggcccgtgc gaggcagcag atgcgggcat
     1501 cgagcccgag cgaggcgacg gcgctgacgc accgtttctc gtacggcgag gttgccggga
     1561 acccggcctc gatgacctcc accccgatct ggtccagtac ctctgcaatt ctcgtcttct
     1621 cctcgcaggt gaaggagact ccaggtgtct gctccccgtc gcgaagggtg acgtcacata
     1681 tctcgatatg ccacgatctc atgttcctgc ccctccgggc atgttcccct gatcacaatc
     1741 actccgggcc gcgcagcatg ctgcagctgg tgccggatct cttcaatatc ctgtacatct
     1801 accacgtgcg ggtcggccca gccaaggtac cgctcgaccg ggagggacgg ctgcttccct
     1861 gtcatcgcgg cacagcggtt gtcgaggatt acgcagagga gggaaagccc cttcgcgtat
     1921 acgtccatca gcgcgtttat cccgctgtgg aggaaggcat agtccccagt gagggcaaca
     1981 cccgtgctcc tggccgccac ggccacgctc gatcccatcc cgtaactggc cacccctatg
     2041 caataggggg ggttcattgc cagcaccacg cacccggcat cgcagaccgg ccgcatcccg
     2101 agatcctgca tgagggagat aaccggtttg aacgggcagt cgcggcagaa cgtcctgtaa
     2161 tacccgcgtt cctcgagcgt ctgcggcggg gcccggcctt cctcctcagg gaggatctcg
     2221 tggcggcagt ggtccttcgt gaaggtgagg ccggcctccc tcacctggcg gaattctgca
     2281 agccttgccg gaggcggaaa gacagtgacc acgcggctcc tgccgggctc cgggcctacc
     2341 cccgcgggct ctccgcacca ggcattcagc ggcgatgcat ccgcccattc ctgcattttc
     2401 cggaagaggg tgcgtgaagc catcaccctt ccgtgcatgg tgtaactcct gtccgagagc
     2461 cgccctgtgg agtctttccg tggcacaggg ctgtcggttg cctctccttc aaggaacccg
     2521 ggggtgaccc tgatcattgc caccctcgag aactcctcag atgcctggag ggcggcctcc
     2581 accgcccgga acgcgctgcc ggaatccggc tcgatgaccg gtatctctgc aagctccccg
     2641 atgatcctcg tgtcctgtga tgtctgcgag cccacggcat cggggtcatc ccctgccacc
     2701 agcagcacac ccccgacaag gccctgggcg gttgcctgca ggagcgggtc cacgcacgcg
     2761 ttgactccca cgttcttcac gatcaccgcc gcccgcctcc ccgagagaga atcgccgagg
     2821 gcgtattcaa ggccggtctt ctcgttgatc accatctctg ccccggtcat tggaccgagt
     2881 tccgtgacag gatacccggg gaccgtatac cgccggtcag ttgcgtcaag gattgcgtgt
     2941 gcaagtgctt cgaccccttt catgtatcgt ccctcccagg caccaggtcg cacccggtgc
     3001 aggcaacgca catcgtcaag gccgcgcccg ggtcgagccc cgcctcgcac agcgcctctg
     3061 catggatcct gttgatcgag aggatccgct cctttgaagg gcgccggtat cccctgcacc
     3121 ctgccgcagg cgagaggggc cggaccaccg gtatcacgcc catccgggtg aggtcctgaa
     3181 tgcattcctc cacctgttcg tccgtctccc cgaggccgat gataaggttc gagaagaccc
     3241 ggtttttccc aaagaggggg actgactgcg cgagcgcccg ccatatgtcg tcccgggaga
     3301 ggccccggca catcttctca aagagtgcgt cggttgcggt ctcaacgttg aacttcacct
     3361 ctctcacacc gagggcgcag agccgctccg cactctcgtt cgtcgggtaa atggagactc
     3421 cgatggggat gccgaacttc ccaagctttt gcaccacgtc gaggacatac ctttcctcct
     3481 cctccacgct ccccatcacg ccgcttgtca gggagatggc gtcgattcga tctgcgactc
     3541 cctccaccat ggccacgatc tcatcgatcg actttctcct cgggggatgg ccgggcacct
     3601 cgcagtacct gcaggagaag atgcacccgc tgctgaccgt gatgtatgcc tgcctcgggc
     3661 agtgaagtgc gatcttctcg aggctccccg cgatctcttt tccatccacg gagagcactg
     3721 cctcaccccc tcccctgtgc atgacccgga tgctgctttc cgggtcaatg gccaggcgga
     3781 cgcgccttcc cccgtgggag aagaataccg aaccggggcc ccgtgcaccg ggccccgcgg
     3841 tggaggcgga gatgtagccg tccgcaggct ctccctccag tcgggcggcc cccgcctcga
     3901 ggagcctggc tttcagcatg ttccatcgca cagctgcagc gcctcctcga accgggtgga
     3961 gaacgggatg gcggccactg caccgatgat tcccctcccc tccatgagat attccaggtc
     4021 tctgcccttc agcgccgcga ggcgctccct gttcacctcg ccgcgcttga ctgcccaccc
     4081 gtactcaccg agcggagcgg gatcgaaccc cgtgaacact gccatcccgg tcttctcgga
     4141 gagggtgtaa cgttcgagaa gccgctggaa cgatgagacc agccgggccg ggtcgcgggt
     4201 cgcgaactcg cagacgacgg cgacgcagtt cttggtccgg tacgggaccg ggaagagctg
     4261 gacgatggtg tgggagagat accgggagtc tgcatcctgt acggcgcttg cgatgttgtg
     4321 gcagagagtc catgtggcgc cctcctccgg ggtgtcagta tcgtcaaccc cgatcaggac
     4381 gcgctcgcgg cgggggagcc agagagttgc cccggctttc ttcccaccgc ctgaagggtc
     4441 gcattcggat ctcagcaccc cccctgcgag ggagcggcag cccgacgccc ccactccgcc
     4501 tccgccaagg cctacgtagg agactgcgat ctcgtcgcgg ctcacctcca ccccgcagat
     4561 accggccggg aacctcgatc cctcaagtcc aaggtccacc tgcccgggag agaggagata
     4621 ccgggtgctc cctcccacgg tccggaccga gtgcacgagg gggctctttg catagtgaag
     4681 gcgtgcccac atggctccgc cgacgcactc gaagaactcc accagttcga cctgtgttcc
     4741 atcccgggag gatagtgcca ctatctcagg atacctgatg acgtacgggt ctttgaggct
     4801 ctcctgccct gcgttttccg ggtctctcat gatccacctg gagccacggt gcctggtgcg
     4861 gaacgcgggg ggcaaccatg gggcgtccat cccggctgcg cccggttgcg ctgccatgct
     4921 tcctggaaca ctggttctcc cctcatcgcc aggactacat gaatcgcagg cgatcccggc
//
LOCUS       DAOQVJ010000052         4977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_18723_length_4977_cov_4.883381, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000052 DAOQVJ010000000
VERSION     DAOQVJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18723_length_4977_cov_4.883381"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..699)
                     /locus_tag="PKL26_01895"
     CDS             complement(1..699)
                     /locus_tag="PKL26_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KaiC domain-containing protein"
                     /protein_id="HNS82477.1"
                     /translation="MSEGRVKIGIEGLDQMMGGGLIPGSICSIIGTYGTGKTTFALEF
                     IWEGLKKGENVIYISLEEREDRILRYMDEKGWDIAPYRGHALSVLKLDPSDFNLAINS
                     IKNELPALVKQVGAQRVVIDPVSLFEDLFDRDSDRRREMFRFVDTLRDQHCTIVMTSE
                     TDKDNPFASRHNLIEYLADTVILLRYVRPSDLTDVHLALEVVKMRLSAHSREIKPYEI
                     QQGQVLVYTEANVF"
     gene            complement(696..1520)
                     /locus_tag="PKL26_01900"
     CDS             complement(696..1520)
                     /locus_tag="PKL26_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNS82478.1"
                     /translation="MHDTARRTMPTGISSLDAVMEGGMPPGSLILLLGDIGGGNNEFV
                     YSSIMLLSVMKQKPAGEDFLLPEEIDYITFTKGRSDILEEMKRSFNPDLTQALGDVKF
                     KDLSQIYFDSSIIPLEWYSDKDIISRLQSRGGHENILAYLAAQLNTVPKNSLVVLDSI
                     TDLATQFNKIGKWNDFSAFLRGLQRVAKEWNTTVYLLLTKGILDAPRELEVADASDAV
                     LFFKWEETTGARRQRVMYIEKFRGLMPHLEEKDLVKFAVRITSAEGFDVSNIRVVI"
     gene            1724..3388
                     /locus_tag="PKL26_01905"
     CDS             1724..3388
                     /locus_tag="PKL26_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNS82479.1"
                     /translation="MPLKVLDLKGIADRLKPVKGKDEIDTTPHDFSITRVISRKKSSA
                     EMPEYDFSGHGPLVDAELPPGYREVERYWVDEGRSLVDIALNENTNQYEYLLYEPSLS
                     EFEYELLERIHEDMRSALILTDEELGKDRRALLMEKMHVLLHEYGITLEERSLFCLQY
                     YLVRNFIGWGRIDALMRDPYLEDISCDGHRIPIFLYHRKYRNVKTNIMFEAEVLNSLA
                     ITLAQRSGKHISTGTPMLDATLPDGSRLQLTLGTEVTTRGTSFTIRKFREDPFSPVEL
                     MEYGTFSSDELAYFWMAIENNKSLLFIGGTASGKTSSLNAVSLFIPPMAKVVSIEDTR
                     EITLFHENWIASVTRETVAEGSNIISMFDLLRAAMRQRPEYILVGEVRGVEAQTLFQA
                     MNTGHTTFSTMHAGSVDAAIHRLENEPLNVPRNMVTALNIVSVQALVYRGMERVRRCL
                     EIVEIAGIDPSTGNLRVNTVFVYDPIRDTFSYKGRSQIYADIAERRGWSRERLEEEIN
                     TRKKILDAMQKQRIIDYIRVSRIFHAYSINPSRVTENLEDLSRILE"
     assembly_gap    3564..3663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3676..>4977
                     /locus_tag="PKL26_01910"
     CDS             3676..>4977
                     /locus_tag="PKL26_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS82480.1"
                     /translation="MQKGNRATKINLTLHNAVAYMYAMRKGGAQLMTIFRSLSENSKI
                     YGEVALEFRQVVRDADFFGYDVVSAIRHLMETTPSEKLNQFLEDMVSVLESGGDLVSF
                     LSGRVRLYQEEARFEQKQFLSFLSLVAESYVTLFVAGPLFLIIIMVVMGMMGGVAILQ
                     LTAVIYLVFPIGSLVFILLIDIISLKGETVERYVRAKWLHEYSDVRVYHREGEEPFFA
                     RLVHYDRVRNAREFFRHPFDAFQKNYNLTLFITVPLAIIYVVAVFWVVPQYQDLEIYI
                     DVVDDHVMIALLIVMAPYAFFYEMWRRNVQGMESLIPEFLDRMAGINQVGLTIAQAIG
                     IMVNTNLGLLSYEIKRIKRDIDWGASFTDALIRFEDRIRTPLIARTVTLITKASQMSG
                     SIGDVLAIAASDAKMSDLLKRERLSEMFIYTAIIYLAFVVFV"
BASE COUNT         1066 a         1435 c         1349 g         1027 t
ORIGIN      
        1 tcaaaacacg ttcgcttccg tatacaccag cacctggccc tgctggatct cgtacggctt
       61 gatctcccgt gaatgggcgg agaggcgcat cttgaccact tcgagcgcaa ggtgcacgtc
      121 ggtcagatcc gaggggcgga cgtagcgcag gaggatcacc gtgtcggcga ggtactcgat
      181 gaggttgtgg cggctggcga agggattgtc cttgtcggtc tcgctcgtca tcacgatagt
      241 gcagtgctgg tcccgcaggg tgtcgacgaa ccggaacatc tcccttctcc tgtcggagtc
      301 gcggtcgaag agatcctcaa agagtgagac cgggtcgatt acgacccgct gcgcgccgac
      361 ctgcttcaca agggcaggga gttcgttctt gatgctgttg atggccaggt tgaagtcgga
      421 gggatcgagt ttcagcaccg agagggcatg ccccctgtag ggggcgatat cccacccttt
      481 ctcatccatg tagcgcagga tccgatcctc ccgttcttcg aggctgatgt agatgacatt
      541 ctcccctttc ttgaggcctt cccatatgaa ctccagggca aaggtggtct tccccgttcc
      601 atacgtgccg atgatggaac agatgctccc cgggatgagg cccccgccca tcatctggtc
      661 aaggccctcg atcccgatct tcacccgacc ctcgctcata tgaccacccg gatgttgctg
      721 acatcgaacc cctcggcaga ggtgatccgg acggcaaact tcaccaggtc cttctcctcg
      781 agatggggca tcaacccgcg gaacttctcg atgtacatca ctcgctgccg ccttgccccc
      841 gtggtctcct cccacttgaa gaagaggacg gcatccgacg catctgccac ctcaagttcc
      901 cgcggggcat ccaggatccc cttcgtgagg agaaggtata cggtagtgtt ccactccttt
      961 gccacccgct gcaacccgcg taggaacgcc gaaaaatcat tccacttgcc aatcttgttg
     1021 aactgggttg ccaggtcggt gatggagtcg agcactacca ggctgttctt tggcaccgtg
     1081 ttcagctgcg ctgccagata cgcaagaata ttttcgtgcc ccccccttga ctggagcctg
     1141 gatatgatgt ccttgtccga ataccattcc aggggaatga tactcgaatc aaagtagatc
     1201 tgggagagat ccttgaattt cacatctccg agtgcctggg tcaaatcggg gttgaaggac
     1261 cgcttcatct cttccaggat gtctgatctg cctttggtaa acgtgatata atcgatctcc
     1321 tcgggaagca ggaagtcctc ccctgccggc ttttgcttca tcacggagag gagcatgatg
     1381 gaactgtaga caaattcgtt gttccctccc ccgatatccc caagaaggag gatcagcgac
     1441 ccggggggca ttcccccctc catcacagcg tcgagtgatg aaattccggt gggcattgtc
     1501 cggcgcgccg tatcgtgcat gcagaacctc tgttattcca tgaagagtga gggtccgccc
     1561 atacatgtag atttctgtca gacgggcaag aaaatggctc cagacgcggt tgaagacctc
     1621 attgcaatgg tcccccaatc atgcttttat ataggtgtgc agggatacca atcctacaga
     1681 agagaacgcg cccagggcct ggtcgcaggg agtggggaat tccgtgccgc tgaaagtttt
     1741 ggacctgaag ggaattgccg accggctgaa gccggtgaag ggcaaggacg agattgacac
     1801 cacgccgcac gatttttcca ttacgcgtgt aatatcccgt aaaaaatcct ccgcggaaat
     1861 gccggagtat gacttttcag gccatgggcc cctggtagat gcggaactgc cgccaggata
     1921 ccgtgaagtc gagcgctact gggtggacga gggcaggtcc ctcgtcgaca tcgcactgaa
     1981 cgagaacacc aaccagtacg aatacctcct ctacgagccg agcctctctg agttcgaata
     2041 cgaactcctc gagcggatcc acgaggacat gcgttccgcc ctcatcctca cagatgaaga
     2101 gctcgggaaa gaccggcggg cgctgctgat ggagaagatg cacgtactcc tgcacgagta
     2161 cggcatcacg ctcgaggaaa ggagcctctt ttgcctccag tattaccttg tccggaactt
     2221 catagggtgg ggaaggatcg acgccctcat gagagaccca tacctcgagg atatctcctg
     2281 cgacgggcac cgcatcccca tattcctcta ccaccggaag taccggaatg tcaaaacaaa
     2341 catcatgttc gaggcggaag tactcaactc cctcgccatc accctcgccc agaggtccgg
     2401 aaaacacatc tcgaccggga cccccatgct cgatgcaacc cttcctgacg gctcaaggct
     2461 ccagctgaca ctcgggacgg aggtgaccac gaggggaacc tcgtttacca tacgcaagtt
     2521 ccgcgaggat ccattctctc ccgtggaact gatggaatac ggcacgttca gttcggacga
     2581 gctcgcatac ttctggatgg ccatcgagaa caacaagagt ctcctcttca tcggggggac
     2641 cgcgtcggga aagacctctt cgctgaacgc cgtctccctc ttcatccccc caatggccaa
     2701 ggtcgtgagc atcgaggata cccgcgagat caccctcttc cacgagaact ggatcgcgag
     2761 cgtgacgagg gagacggttg cagaggggtc aaacatcatc tcgatgttcg atctcctcag
     2821 ggctgccatg cgccagagac ccgaatatat cctggtcggc gaggtcaggg gcgttgaagc
     2881 acagaccctc ttccaggcga tgaacaccgg gcacaccaca ttctcgacca tgcatgcagg
     2941 gagcgtggat gcggccatcc accgcctcga gaacgagcca ctcaacgtcc cgaggaacat
     3001 ggtgaccgcc ctcaacattg tcagtgtcca ggccctggta taccgcggca tggagagggt
     3061 gcgcaggtgc cttgagatcg tggagatcgc cgggatagat ccttcgaccg gaaacctcag
     3121 ggtcaatacc gtcttcgtgt atgatccaat ccgtgataca ttcagctaca agggcagatc
     3181 gcagatttat gcagacatcg ccgagcggag ggggtggagc agggagaggc tcgaagagga
     3241 gatcaacacc cgcaagaaaa ttctcgacgc catgcagaaa cagaggatta tcgactacat
     3301 cagggtatcc aggatcttcc atgcctacag catcaacccc tcccgcgtga cagagaacct
     3361 cgaggacctc tccaggatac tggaatgatc ttcaacgccc tgatcaggag gagaatccag
     3421 cgcaatcccc tgcgttacca ggatctccag tccgaactca tctccacccg ggctggactg
     3481 acgctcgagc gttaccttat aaggtcgatg gaagttgccc tcctggcagg cgcaggattt
     3541 gcggtcctcg ggtacatcct gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnaagtatc ccggcatgca gaagggaaac cgggcaacca agatcaacct caccctccac
     3721 aacgcggtgg cctatatgta tgccatgcgg aaaggtgggg cgcagttgat gacaattttc
     3781 cgctccctct ccgagaactc aaagatttac ggggaggtgg ccctcgagtt ccggcaggtc
     3841 gtgagggacg cggacttctt cgggtacgac gtggtatccg ccatccgcca cctgatggaa
     3901 accactccct ctgagaagct gaaccagttc ctggaggaca tggtctcggt gctcgagagc
     3961 ggcggggacc tcgtgagctt cctgtcagga cgtgtcaggc tctaccagga agaggcaagg
     4021 tttgagcaga agcagttcct cagtttcctc tccctggttg cggagtcgta tgtcacgctc
     4081 ttcgttgccg ggccactctt cctcatcatc atcatggtgg tgatggggat gatgggcggg
     4141 gtggccatcc tccagctcac cgcggtgatc tacctcgtgt ttcccatcgg gtccctggtc
     4201 ttcatcctcc tcatcgatat catctccctc aagggtgaga ccgtcgagcg atatgtccgt
     4261 gcgaaatggt tgcacgagta cagtgatgtc agggtctacc acagggaggg ggaggagccg
     4321 ttctttgccc ggctcgtcca ttatgacagg gtgaggaatg cacgcgagtt cttccgccat
     4381 cccttcgacg ccttccagaa gaactacaat ctcacgctct tcatcaccgt cccccttgca
     4441 atcatttacg tggttgccgt attctgggtg gtgccccagt accaggatct cgagatctac
     4501 atcgacgtgg tggacgacca cgtgatgatt gcactgctca ttgtcatggc cccctacgca
     4561 ttcttctacg agatgtggcg ccggaatgtc cagggcatgg aatccctcat cccagagttc
     4621 ctggacagga tggcggggat taaccaggtg ggcctgacaa tcgcccaggc catagggatc
     4681 atggtgaaca ccaacctcgg tctcctctcc tacgagatca agcggatcaa gcgggatatc
     4741 gactggggag cgagtttcac agatgcgctc atccgcttcg aggaccgcat ccggacaccc
     4801 ctgatcgccc gcacggttac ccttatcacc aaggccagcc agatgagcgg atctatcggg
     4861 gacgtgctcg ccatcgcggc aagcgacgcc aagatgtccg atcttctgaa acgggaacgg
     4921 ctctcagaga tgttcatata caccgcgatc atctaccttg cgttcgtggt attcgtc
//
LOCUS       DAOQVJ010000053         4892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_19165_length_4892_cov_3.886293, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000053 DAOQVJ010000000
VERSION     DAOQVJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19165_length_4892_cov_3.886293"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKL26_01915"
     CDS             complement(<1..810)
                     /locus_tag="PKL26_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS82481.1"
                     /translation="MNTITHPFIAPGRLESRTYQLSIAMKALEGHTMVVLPTGLGKTA
                     IALLVAASRLYNQGGRVVMMAPTKPLVEQHLRFFRQFLSLPSQEDDTLAMFTGETPSG
                     ERAAAWKKARMVFATPQVIKNDVLAGSYDTRDVSLMIFDECHRAVGNYAYVFLAQRYL
                     ETAASPLVLAMTASPGGQVEKVEDVCRNLGIVKVESRTEHDRDVAPYIHEREIEVVEV
                     DLPPELKQAASDLNILVDSRLSQLTQLGFRVPPRQQLSMKALQELNAQVQEQ"
     gene            complement(807..>1934)
                     /gene="glyS"
                     /locus_tag="PKL26_01920"
     CDS             complement(807..>1934)
                     /gene="glyS"
                     /locus_tag="PKL26_01920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNS82482.1"
                     /translation="PRQGMIRLREFTQAEAEIFVHPDEKDHPFFSRYAGYRMPLLGIR
                     QQQEETPALETTMDEAVREGLIANQYVAYYLALTHHLLVTVGVDPEKLRFRQHLPDER
                     AHYAEDCWDAEIFSERFGWVETVGIADRTDYDLRAHEKQSGQRFAVFIPYETPRKERR
                     VRVVANMGALGPRFRGKAKAVADALADVVPGPDGVPLTIDGEEVFISPDLYQVREEEV
                     EIPGEEITPHVIEPSYGIDRMIYAVLEHRYAEETVNDEERRVLRLPPRIAPIQAAVFP
                     LMNRDGLPEIARDIVTSLKARGIMAEYDDSGAIGRRYRRQDEVGTPFAITVDYETPET
                     GTVTLRDRDSMEQVRVPAETLPDLIRSLVEGSATFASLSPA"
     assembly_gap    1935..2034
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2035..2686)
                     /gene="glyS"
                     /locus_tag="PKL26_01925"
     CDS             complement(<2035..2686)
                     /gene="glyS"
                     /locus_tag="PKL26_01925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNS82483.1"
                     /translation="MSDIYDKIMDLAKRRGFIWPASECYGSVAGFIDYGPLGSMMKRR
                     IEDTWREFYVVREGYYEIECPTIGQEDVFIASGHVKGFSDKMCQCPHCREYLRADHIA
                     EGQGIPGASTMDTGELSRVLSTLPCPVCGEVLGEVEVFFFNLMFQTSIGPGSQRKGYL
                     RPETAQGMFTDFQRLLRFYRDTLPFGAIQIGKSYRNEISPRQGMIRLREFTQAEAEI"
     gene            2903..3511
                     /locus_tag="PKL26_01930"
     CDS             2903..3511
                     /locus_tag="PKL26_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HNS82484.1"
                     /translation="MQDDKRKKELLQLFTNISGKTKIVEPMKKIHGTLRDRDAVEREI
                     ALVMREILEQGFFKTKLKPIQLARLVSAYYAGKNDTEIARELGDEKLSKTVARARVRL
                     KLFRELDFKMPFDQDRMEDLLISGKTMKEVSEDLGVSPSTLREYRHVIEQRDDVTIDP
                     FLERIKDVMEDRDLSEQMTRSATKDSLGEAIDITEAEMIDVT"
     gene            3582..4235
                     /locus_tag="PKL26_01935"
     CDS             3582..4235
                     /locus_tag="PKL26_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS82485.1"
                     /translation="MKLTWFGHSCVLLEGSRKVLIDPFIPEGSIDVEPDVVAVTHGHA
                     DHMGDAASYGVPVVAINEVAKYLRTKGVEAEAMNIGGTIEVKGVRFTMTPALHSAWIE
                     EAGIGCNGGCAAGFVIRMDGKAIYHAGDTGLFSDMSLIGELYHPDVALLPIGGRFTMG
                     PEEAMVAARMIGSPLVIPIHYNTWPAISQDAEGFKRTLERTTDCRVRVLAPGGSITL"
     gene            4287..4478
                     /locus_tag="PKL26_01940"
     CDS             4287..4478
                     /locus_tag="PKL26_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82486.1"
                     /translation="MHDTFLAANRIKREPSSDILILSHWNFDAWKSVCKMELDGKTVI
                     GAGVVLFVIVVGIWYMFLR"
BASE COUNT         1016 a         1325 c         1449 g         1002 t
ORIGIN      
        1 ctgttcctgc acctgcgcat tgagttcctg gagggccttc atggagagtt gctgccgggg
       61 cgggacacgg aacccgagtt gtgttaactg ggaaagccgc gagtcgacga gtatgttcag
      121 gtctgatgct gcctgcttca gttcgggcgg gaggtcgact tccacgacct cgatctcccg
      181 ctcgtggatg tagggggcca cgtcccggtc gtgttcggtc ctgctctcga ccttgacgat
      241 cccgaggttc cggcagacgt cctcgacctt ctccacctgg cccccgggcg aggctgtcat
      301 tgccaggaca aggggtgagg ctgcggtctc gaggtaccgc tgcgccagga agacgtaagc
      361 gtaatttccg accgcacggt gacactcgtc gaagatcatc agggagacgt cccttgtgtc
      421 gtaactgcct gcaaggacgt cgttttttat cacctgcggc gttgcaaaaa ccatcctggc
      481 cttcttccag gcggccgcac gttctcctga aggtgtctcc cctgtgaaca ttgccagggt
      541 atcgtcttcc tgcgagggga gcgagaggaa ctgccggaag aagcggagat gctgctccac
      601 caggggcttg gtcggggcca tcatcaccac cctgccgccc tggttgtaga gcctcgaggc
      661 cgcgacaaga agtgcgatgg cggtcttccc gagtcccgtg ggcagcacga ccatggtgtg
      721 cccctcgagc gctttcatcg cgatggagag ctggtaggtg cgcgactcca gcctgccggg
      781 cgcaatgaag gggtgggtga tggtgttcat gcaggggaga gggatgcgaa ggttgcggac
      841 ccttccacga gggaccggat gagatctggg agggtttcgg ccgggacacg cacctgctcc
      901 atgctgtcgc ggtcccggag agtcactgtc ccggtctcgg gagtttcgta atcaacagtg
      961 atggcaaagg gagtcccgac ctcgtcctgc ctccggtacc gacgcccgat ggctccggag
     1021 tcgtcgtatt cagccattat ccccctggct ttgaggctgg tgacgatatc acgcgcgatc
     1081 tcggggagcc cgtcgcggtt catcaggggg aagaccgccg cctggattgg ggcgatgcgc
     1141 gggggaaggc gcaatacccg gcgttcctcg tcgtttactg tctcctcggc atagcggtgc
     1201 tcgagcaccg cgtagatcat ccggtctatg ccgtaacttg gctcgatcac gtggggtgtg
     1261 atctcctctc ccgggatctc aacctcctcc tccctgacct ggtagaggtc gggggagatg
     1321 aagacctcct cgccatcgat ggtgaggggg actccgtccg ggccggggac cacgtctgcc
     1381 agtgcgtctg cgaccgcctt tgccttcccc ctgaaccttg ggccgagggc gcccatgttg
     1441 gcgaccacgc gcacccggcg ctccttccgc ggggtctcgt aaggaatgaa caccgcaaat
     1501 cgctgcccgc tctgcttctc gtgcgccctt aagtcgtagt cggtgcggtc cgcgataccc
     1561 acggtctcaa cccagccaaa ccgctccgag aagatctccg cgtcccagca gtcctctgca
     1621 tagtgggcgc gttcgtccgg gaggtgctgg cggaagcgga gtttctccgg atcaaccccc
     1681 acggtcacga gaaggtgatg ggtgagggca agataatatg ccacgtactg gttggcgatc
     1741 agcccctcgc gaacggcctc atccatggtg gtctccaggg caggggtctc ttcctgttgc
     1801 tggcgtattc caagcagggg catgcggtac ccggcgtacc gggagaagaa cggatggtcc
     1861 ttctcatctg gatggacaaa tatctctgcc tcagcctggg tgaactcccg gaggcgtatc
     1921 atgccctgcc tgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatatct
     2041 ctgcctcagc ctgggtgaac tcccggaggc gtatcatgcc ctgcctgggg gagatctcat
     2101 tcctgtagga cttgcctatc tggatcgccc cgaacgggag cgtatcgcgg tagaaacgca
     2161 ggaggcgctg gaaatcggtg aacatcccct gtgcggtctc ggggcggagg taaccctttc
     2221 tctgggaacc ggggccaata gaagtctgga acatcaggtt aaaaaaaaag acctccacct
     2281 cgccgagcac ctcgccgcag acaggacagg gtagggtgga gagcacgcgg gagagttcac
     2341 cagtgtccat ggtcgaggcc cccgggatcc cctgcccttc cgcgatatgg tcggcacgga
     2401 ggtactccct gcagtggggg cactggcaca tcttgtccga gaaacccttc acgtgccccg
     2461 aggcgatgaa aacgtcctcc tgccctatgg tggggcactc gatctcgtag tacccctccc
     2521 ggacgacata gaattccctc cacgtgtcct cgattcgcct cttcatcatg gagcccagag
     2581 ggccgtaatc gatgaatccc gcgaccgaac cgtaacattc ggacgccggc cagataaagc
     2641 cccttctttt ggccagatcc attattttat cgtatatatc gctcatggac acacttctct
     2701 cttatatctg gtgctgcggc cagaaaaact gtgcgggaag gcgcatgtct ttcgggggag
     2761 ataccctttt ctcagatccc agggaactgc ctccatatgt ccgtatctcc atgataaagc
     2821 cttaaagctg gagagcgggg cagaaggata gtgttatata tattgctggg tatcttattc
     2881 gataattcac gagcgaggtt tcatgcagga cgacaagaga aagaaagagc tgctccagtt
     2941 gttcaccaat atatcaggga aaacaaagat cgtggaaccc atgaaaaaga tccacgggac
     3001 cctccgggac cgtgatgccg tggagcgcga gatcgcgctg gtcatgcggg agatcctgga
     3061 acagggtttt tttaagacaa aactcaagcc catccagctg gcaaggctgg tatctgcgta
     3121 ttacgcgggg aagaatgata ccgagatagc ccgcgagctc ggggacgaga aactttcaaa
     3181 gacggttgcc cgtgccaggg tgaggctcaa gctctttcgc gaactggact tcaagatgcc
     3241 gtttgaccag gaccggatgg aggatctcct catctccggc aagacaatga aggaggtgag
     3301 cgaggatctc ggggtcagcc cgtccacact tcgggaatac cggcatgtga tcgagcagag
     3361 ggacgatgtc acgatcgatc cattcctcga gcggataaag gacgtgatgg aggacaggga
     3421 cctctccgag cagatgaccc ggagcgccac caaggacagc ctcggggagg caatcgacat
     3481 caccgaggca gagatgatcg acgtcacgtg aagatgccaa tacccttttt ttcccgcgcc
     3541 aacccaatat cccgtaccgg ggcatagagg gagggaccca gatgaaactc acatggtttg
     3601 gacattcgtg cgtgctcctg gaagggagcc ggaaagtgct cattgatccg tttatccctg
     3661 aaggatccat tgatgtggaa ccggacgtgg tagcggtcac ccatgggcat gccgatcaca
     3721 tgggggatgc ggcgtcatat ggagtgccgg tggtcgccat caatgaagtg gcgaagtacc
     3781 tgcggacgaa aggtgttgag gcagaggcca tgaacatcgg gggcacgatc gaggtgaagg
     3841 gcgtgcggtt caccatgacc cctgcactcc actccgcctg gatcgaagag gccggtatcg
     3901 gatgcaacgg cggctgtgcg gcagggttcg tcattcgaat ggatggaaaa gcaatatatc
     3961 atgccggcga tacggggctc ttctcggaca tgtccctgat cggggaactc taccacccag
     4021 atgtggcgct cctccccatc ggggggaggt tcacgatggg cccggaggag gccatggtcg
     4081 cggcccggat gatcggctct cccctggtga tcccgatcca ttacaacacc tggccggcga
     4141 tctcacagga tgcggaaggg ttcaagcgta cgctcgaaag gacaactgac tgcagggtca
     4201 gggtacttgc accgggagga tcaattactc tttaaaggaa cgttcccgtc aagagttcgg
     4261 ggagtactcc cgaacatccc ctgcagatgc acgatacatt tctcgcagcg aaccggatta
     4321 aaagggaacc ctcatcagat atccttatct tgtcccactg gaattttgat gcatggaaga
     4381 gtgtgtgtaa gatggaactt gacggaaaga cagtgattgg tgcaggcgtt gtcctgtttg
     4441 tgatcgtagt cgggatatgg tacatgttcc tcaggtgacg gaagcgtaac agatcccggt
     4501 tgaagaacaa cacggtccct catttacctt ttttcatcca gttctcctgt atcccgcagg
     4561 tatggcgatc cccataagag gctgcgcgta acccccgcat tggaaccgga ttaacggcat
     4621 gcgatgttga taattgtaat ctataaccaa ctaatagtaa ggtatattta acaatatgtt
     4681 aaacctaatg ggcatatgca cgtgataaca ggtgaatatc aaattaggag gtccatccca
     4741 aggatctcat ccctggttct tgtgcttgcg atatgcacat tctgcaactg catgattgca
     4801 gttgcggacg ccacgaatgg tgagggaacg gagcaggcta tcggaataga catgtccgat
     4861 gcagcccata gcagcaaggc agaacccaat ta
//
LOCUS       DAOQVJ010000054         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_20700_length_4630_cov_4.401093, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000054 DAOQVJ010000000
VERSION     DAOQVJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20700_length_4630_cov_4.401093"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKL26_01945"
     CDS             <1..541
                     /locus_tag="PKL26_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase subunit GuaA"
                     /protein_id="HNS82487.1"
                     /translation="IYPDRIESEGGIKSHHNVGGMPLHMEFETVIEPLRDLYKDEVRV
                     LAGALGLPHEIQHRMPFPGPGLAVRVIGEVTRENIEVVRQANWIVEREIVEQFNPWQC
                     FAALLGLGTGVKGDNRVHGWVVAVRAVNSRDGMTADPVQIPWDVLDRIASRITSEIPS
                     VARVVYDITPKPPATIEYE"
     gene            570..1700
                     /locus_tag="PKL26_01950"
     CDS             570..1700
                     /locus_tag="PKL26_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HNS82488.1"
                     /translation="MQNKEDYRAIEARYYMPAFARDLMIVRGEGSRVWDADGKEYIDC
                     VAGIAVCSTGHCHPAVTRAICEQAKRLIHCSNLYYVPGQAELAQKLVEKTGMAKAFFS
                     NSGAEATDGALKLARVRTGRKKFVAFTHGFHGRTIGSLAVTHKPAIREPFEPLGISKT
                     FVEYGDIDGLRKVVDSDTAGVVVEPIQGEAGVIIPPDSFLHGIREICDESGALMIMDE
                     VQTGMGRTGKWLAIQHAGVKPDIVMLAKGIASGFPMGALLAREGLEFRRGEHGSTFAG
                     GPLACAAALATFGVIDEIVPTVSEKGERFRNALSKYAPRARGLMVGMTVGDRCAEVQK
                     RCAENGVLVNCAADGNLRLVPPLVITNAEIDRACSVLKDALG"
     gene            complement(1713..2330)
                     /locus_tag="PKL26_01955"
     CDS             complement(1713..2330)
                     /locus_tag="PKL26_01955"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNS82489.1"
                     /translation="MEQNRLYTLPALPYDYKALSPHISEEQLRIHHTKHHQGYVNGAN
                     AIYEKLDKARREGSDLDVKAVHKELSFHIGGFLLHALYWENMAPAGKGGGAPKGPLSA
                     HIEKDFGSFERFKKEFTTAANSVEGSGWAALTFCQKTGRLLLMQIEKHNVNVYPAFPI
                     LMAIDVWEHAYYVDYKNDRAKYIDTFWGIVNWDTVNARLEKVLKK"
     gene            2528..3607
                     /gene="hisC"
                     /locus_tag="PKL26_01960"
     CDS             2528..3607
                     /gene="hisC"
                     /locus_tag="PKL26_01960"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNS82490.1"
                     /translation="MAKENDSRYTSLVRDCYRSGGYVFARRAGNTGEDDEACRVARLA
                     SNENPRPPSPLALERGCQALREVNRYPDERMADLVRALAKFHGQYHFVTGVGMDGVIE
                     TVIRTLVCPGERVAVSTPTFSFYGLAAMAQGAEVVNSPRRPDFSVDVHQFVRSARDAK
                     ISFLCTPNNPTGNVTSRDDVEEILSAIHGVLFLDNAYVEFCDEDYRPLMNRYENLILG
                     RTFSKVFSLAGLRVGYAFVPLWLVPCYLRAATPFTVNSVSAAAATGALADRLHLKETV
                     EGVKRLRELVVRECKYPVFHSGANFVMVDVSPSSGREMADRLAERGVLVRSCESFPGL
                     GPHYIRVSIGEEWENARFLDVINAL"
     gene            3604..4116
                     /locus_tag="PKL26_01965"
     CDS             3604..4116
                     /locus_tag="PKL26_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HNS82491.1"
                     /translation="MMIGITGTPGTGKSTVAQELMARGYQVTSLSTTTTPYILARDEA
                     RHTLVIDEEAWAREFQKVEGFVEGHLAHLLPCDLIVVLRCRPDVLAARLRARGYPEAK
                     VSENVEAEALDVSLIETLEEFSGDQVLEIDTTERDIGECAGLIEEFARGKMHPSFGKI
                     DWSAYLGDPV"
     gene            4113..>4630
                     /locus_tag="PKL26_01970"
     CDS             4113..>4630
                     /locus_tag="PKL26_01970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNS82492.1"
                     /translation="MTLEILRPQMKEIMAPFISLSKKIGLSPNNLTIGALITAAAAGV
                     FFYLGNVLAGTAFVAFNALLDGLDGALARAENIQGPKGDFLDHVVDRYADIFIITGIF
                     AGGFASWQVGVLALTGVLMSSYLGTQAQAVGVGRYYGGVLGRADRLVLILCAGVLTVF
                     VPDTFFGLSILG"
BASE COUNT          945 a         1263 c         1462 g          960 t
ORIGIN      
        1 catctacccg gacaggatcg agagcgaggg gggcatcaag agccaccaca acgtgggagg
       61 catgcccctc cacatggagt tcgagacggt gatcgagccc ctacgggacc tgtacaagga
      121 cgaggtgcgg gtgctcgcgg gtgcactcgg actcccccac gagatacagc atcgcatgcc
      181 atttccaggg ccgggactcg cagtccgggt tataggcgaa gtcaccagag agaacatcga
      241 ggtcgtcagg caggccaact ggatcgtgga gcgagagatc gtcgagcagt tcaatccatg
      301 gcagtgcttc gcggccctcc tcggactcgg gacaggggtg aagggtgaca acagggtgca
      361 cgggtgggtg gtggctgtca gggccgtcaa ctcgcgcgat ggaatgacgg cagatcccgt
      421 ccagatccct tgggatgtac tggaccggat cgcctcccgc atcacttcgg agatcccgag
      481 cgtggccagg gtggtgtacg acatcactcc caaaccccct gcaacaatcg agtatgaata
      541 agagaacagt tacccagtag tgcgatatca tgcagaacaa ggaagactac cgggcaatcg
      601 aagcccggta ctacatgccc gcgtttgccc gtgacctgat gattgtcagg ggagagggct
      661 cgagggtctg ggatgccgac ggaaaagaat acatcgactg cgtggcagga attgcagtct
      721 gcagcaccgg ccactgccat cccgccgtga cccgcgccat ctgcgaacag gcaaagcgcc
      781 tgatacactg ctcgaacctc tattatgtgc caggacaggc ggaactggca cagaaactgg
      841 tggagaagac cggaatggca aaagcgttct tctcgaactc aggggccgaa gcgacggacg
      901 gggcgctcaa gctggcccgt gtcaggacgg ggaggaaaaa attcgtagcc ttcacgcacg
      961 gatttcatgg aagaaccatt ggatcccttg ccgtgacgca caagcccgcg atccgcgaac
     1021 cgttcgaacc ccttggtata tcaaagacgt tcgtcgagta tggggatatc gacggattga
     1081 ggaaggtcgt ggacagtgac accgcggggg tggtggttga acctatccag ggcgaggcag
     1141 gggtcatcat ccctcctgac agtttcctcc acggcatacg ggagatctgc gacgagagcg
     1201 gcgcactcat gatcatggac gaggtgcaga caggcatggg ccggaccggg aagtggttag
     1261 ccatccagca tgcaggtgtg aagcctgaca ttgtcatgct cgcgaagggc attgcaagcg
     1321 gctttcccat gggcgccctg ctcgcgcggg aaggcctcga gttccgcagg ggagaacacg
     1381 gcagcacgtt tgcagggggt cccctggcat gcgcggcagc gttagccacg ttcggggtaa
     1441 tcgatgagat agtgcccacg gtctctgaaa aaggtgagcg gttccgcaac gcgctctcga
     1501 agtatgcacc cagggcaagg ggcctgatgg ttgggatgac ggtcggggac cgttgtgcgg
     1561 aagtccagaa gaggtgtgcg gagaacgggg tgcttgtcaa ctgtgccgcc gacggcaacc
     1621 ttcgcctggt gccgcccctt gttatcacca atgcggaaat tgaccgggca tgttccgttc
     1681 tcaaagacgc actgggctaa aaacgagtgt atttattttt taagtacttt ttccagcctc
     1741 gcattcaccg tatcccagtt cacgatgccc cagaaggtat cgatgtattt tgcgcggtcg
     1801 ttcttgtagt cgacatagta ggcgtgctcc cagacatcga tggccatcag gatgggaaac
     1861 gccgggtata cattgacgtt gtgcttctcg atctgcatca ggagcagcct ccctgtcttc
     1921 tggcagaagg taagcgctgc ccacccggag ccctcgacgc tgttggctgc agtggtgaac
     1981 tcttttttaa accgttcgaa cgagccaaag tccttctcga tatgagctga aaggggccct
     2041 ttcggggcac cgccaccctt ccccgcagga gccatgttct cccagtagag tgcatggagg
     2101 aggaagccgc caatgtggaa agagagttct ttgtggacgg ccttcacgtc aaggtcgctt
     2161 ccctctctcc gggccttgtc gagtttctcg tagattgcgt ttgcgccgtt cacgtagccc
     2221 tggtggtgtt ttgtgtggtg gattcgcagc tgctcttcag agatatgggg ggagagcgcc
     2281 ttgtagtcgt atggcagtgc cggcagtgtg tatagtctgt tctgttccat gtgtacaacc
     2341 ccatttttac agttgtatct gatttacaca taaaagagtt cgggtcttac cctttcctca
     2401 agcaagatca cacggttgtg ccggcatgat gagggttcga ttgcgagccc ggcacccggt
     2461 ccgcctgctg caacgccctt attaagagtg gagagcttat actggaagca cttacacggg
     2521 gggcggaatg gcgaaagaga atgattccag gtacacctcg cttgtgcggg attgttaccg
     2581 ctccggggga tatgtctttg ccaggcgtgc ggggaataca ggagaggacg acgaagcctg
     2641 ccgggttgcc cgcctcgcga gcaacgagaa ccctcgtccc ccgtctcccc ttgcgcttga
     2701 gaggggctgc caggccctgc gcgaggtaaa ccgctatcct gacgaacgaa tggccgacct
     2761 tgtcagggct cttgcgaagt tccatggcca gtaccacttc gtgaccggcg tggggatgga
     2821 cggggtgatc gagaccgtga tacggaccct ggtatgccct ggggagagag tcgcggtctc
     2881 gacacccacg ttctcctttt acggcctcgc ggcgatggca cagggtgccg aggtggtgaa
     2941 tagcccgagg agacccgatt tctcggttga tgtgcatcaa ttcgtccgca gtgcaaggga
     3001 tgcgaagatc tcgttcctct gcaccccgaa caacccaaca gggaatgtga ccagccggga
     3061 tgacgtggag gagatcctct ccgcgataca tggggtcctt ttcctcgaca atgcgtacgt
     3121 agagttctgc gacgaggatt accgcccact gatgaaccgg tatgagaacc tcatcctggg
     3181 ccggacgttc tcaaaagtct tctctctggc gggcctacgt gtcggctatg cgttcgttcc
     3241 cctgtggctg gttccctgct acctgcgcgc agccacacca tttactgtaa actcggtctc
     3301 tgcggctgca gcaacaggtg cgctcgccga tcggttgcac ctcaaagaga cggtcgaggg
     3361 ggtgaaaagg ctccgtgaac tggtcgtccg ggaatgcaaa tacccggtct ttcattcggg
     3421 tgcgaacttc gtgatggtgg atgtgtcccc ttcgagcgga agggagatgg ccgatcgcct
     3481 cgctgaaagg ggggtccttg tgcggtcctg cgagagcttc cccgggcttg gaccacacta
     3541 tatccgggtg agcatcggcg aagaatggga gaatgccagg ttcctggacg tgatcaacgc
     3601 gctatgatga tcgggatcac cgggacgccg ggaacaggga agtcaactgt ggcccaggaa
     3661 ctcatggcga ggggatacca ggtcacctcg ctctccacca ccaccacccc ctacatactc
     3721 gcaagagacg aggccaggca cacactcgtc atcgacgagg aggcctgggc cagggagttc
     3781 cagaaggtcg agggattcgt ggaagggcac ctggcgcatc tgcttccctg cgatctcatc
     3841 gtcgtgctcc gctgcaggcc cgatgtgctc gccgcccgtc tccgtgcacg cggctacccg
     3901 gaggcgaagg tttccgagaa cgtggaggca gaggcgctcg atgtttccct gatcgagacg
     3961 ctggaagagt tcagcggcga ccaggtgctg gagattgata cgaccgagcg cgatatcggg
     4021 gagtgcgcag ggttgataga agaatttgca cgcggcaaga tgcatccttc gttcgggaag
     4081 attgactggt cggcctatct cggagacccg gtatgacgct cgagattctt cgtccgcaga
     4141 tgaaggagat catggccccc ttcatctcac tctcgaagaa gatcgggctc tccccgaaca
     4201 accttactat cggtgccctg atcactgcgg ctgcagcggg ggtcttcttc taccttggaa
     4261 atgttctggc agggactgcg ttcgtggctt ttaacgctct cctggacggg cttgacggtg
     4321 cccttgcccg tgcagagaac atccagggcc caaaaggcga cttccttgat cacgtggtgg
     4381 acaggtatgc ggatatcttc atcatcaccg ggatatttgc cggggggttt gccagctggc
     4441 aggtgggagt gctggcactc acgggggtgc tgatgtcatc atacctcggg acacaggcac
     4501 aagccgtggg tgtggggaga tattatggcg gagtcctcgg gagggcggat cgcctcgtgc
     4561 tgatcctttg cgcaggggtg ctcacggtgt ttgtcccgga tacgttcttc ggcctctcga
     4621 tccttggatg
//
LOCUS       DAOQVJ010000055         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_20868_length_4602_cov_4.580823, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000055 DAOQVJ010000000
VERSION     DAOQVJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20868_length_4602_cov_4.580823"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKL26_01975"
     CDS             complement(<1..415)
                     /locus_tag="PKL26_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNS82493.1"
                     /translation="MLDRLGTSLKDALKKLAGKTVVDRVAVEELVKDLQRALIQADVN
                     VKMVMQLSQAIKTRSLEEQPPKGMTVREHVLRIVYQELVRLVGSAGEARLEPQVILMA
                     GLQGSGKTTTTAKLARYFQRKGLRVGVICADTFRPG"
     gene            complement(415..1437)
                     /gene="ftsY"
                     /locus_tag="PKL26_01980"
     CDS             complement(415..1437)
                     /gene="ftsY"
                     /locus_tag="PKL26_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNS82494.1"
                     /translation="MFEGLRARLQKVQGTLEKNIAKTASPADTAAVPEECEGAVSGKD
                     PSLIAKVKVLITDREFFIAEKDIEEPLFELEMILLENDVALPVTDEILTRVRADLTGK
                     RRKIGESVDEIVVTALKGALLGVLGEGFDLLAYIRTHEKPVKILFTGVNGTGKTTTVA
                     KVASYLKKNGCSVVIGAGDTFRAGAIEQIDVHAGRIGIKVIQHQAGADPSAVLYDTVQ
                     YAEAHRVDAVLADTAGRFHNRANLMNQLEKIRRVMKPDLVVYVDEAVAGNDAVVRAFE
                     FEKTVGADAVVLTKADMDSKGGAAISIAHTIGKPVMFLGVGQGYDDIIPFSPTRVVDE
                     LLGVEA"
     gene            complement(1460..1918)
                     /gene="pfdA"
                     /locus_tag="PKL26_01985"
     CDS             complement(1460..1918)
                     /gene="pfdA"
                     /locus_tag="PKL26_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HNS82495.1"
                     /translation="MEGIDPRELQSLQLYLNEYRQQAEIFSQQLALLEEGRVEALAAI
                     DAITGIKESSEDTVLLQIGGGVSVRARIEEPERVLLNIGADVVVEQSTEAAVEYLKDR
                     LTEMDASAKKVMETLDQLRYQMNEIARRLEAGYQQYQMQQARPVQGEELG"
     gene            complement(1920..2111)
                     /gene="rpl18a"
                     /locus_tag="PKL26_01990"
     CDS             complement(1920..2111)
                     /gene="rpl18a"
                     /locus_tag="PKL26_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HNS82496.1"
                     /translation="MADQKYEVKGDFRMGDEWRPYAKVVEAPNAAQAKERTFALIGSK
                     HRLKRSYITVQSITLLSGE"
     gene            complement(2119..2781)
                     /locus_tag="PKL26_01995"
     CDS             complement(2119..2781)
                     /locus_tag="PKL26_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HNS82497.1"
                     /translation="MDRTISIAGDSNIGVFTRVIGGLAVVPPVAPAEFQEAIIELLDV
                     EIIPTTIQGSEIVGSLLAGNRRGFVVSGLAHDREIALLEEYGQVYLLEHTMNAAGNVI
                     MANDSFAAVHPDMPREMAGEVGEFLGVRVFPLTLGGIKTVGMAGVATNKGVLVHPRSS
                     RAEIQVLEQAAGVPIGTGSVNRGVGLVGTGLLVNGHGYATGLETTGFELGRVEDVFGF
                     LE"
     gene            complement(2783..3046)
                     /locus_tag="PKL26_02000"
     CDS             complement(2783..3046)
                     /locus_tag="PKL26_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HNS82498.1"
                     /translation="MADKMEEQIYIIPLRDAKRAPRWKRSNTAIKDIRKFLAKHMKSQ
                     DVKLDRTINERVWDRGAEKPPSRIRIRAMKFDDGQVQAELAEE"
     gene            complement(3052..3204)
                     /locus_tag="PKL26_02005"
     CDS             complement(3052..3204)
                     /locus_tag="PKL26_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HNS82499.1"
                     /translation="MSRSTKGYKVRLAKACEQNRRVPAWVMMRTKRQVSTHPRRRNWR
                     RSTLKV"
     gene            complement(3213..3809)
                     /locus_tag="PKL26_02010"
     CDS             complement(3213..3809)
                     /locus_tag="PKL26_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HNS82500.1"
                     /translation="MKAGVLFSGGKDSSLAAVLLSRDYSVELNTFVFSHQRSLAAVER
                     AALALGFPYRRRTFGEELLGRAVEMVLSSGYPNDAIQMVHKEALRILSTEYRVVADGT
                     RFDDRVPMLSRDEVQSLQDTSGCSYLRPLLGYPRREVDRIAARHLEIAFGETGRIENG
                     DYEAEIRDEVRARGEDSGKYFPLHHEQSLVTGTITISG"
     gene            complement(3806..4147)
                     /locus_tag="PKL26_02015"
     CDS             complement(3806..4147)
                     /locus_tag="PKL26_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNS82501.1"
                     /translation="MGDDELAEIRRRRLAQMQQQSIDQQVMQEEMERQKQVEAQIHAV
                     LMQILEPEARERLNTIKLTRPDFARAVEQQLVMLAQSGRLKQKITDTQLKSLLQQLQP
                     AKKDFRITRKG"
     gene            complement(4158..4595)
                     /locus_tag="PKL26_02020"
     CDS             complement(4158..4595)
                     /locus_tag="PKL26_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HNS82502.1"
                     /translation="MTTVYDVPADRFIRTLAEELKKKPEITPPAWAAFAKTGVHKEMP
                     PQDPEWWFVRVASILRRVYIEGPIGVQRMRSIYGGKKDRGSRPSQFRKGSGSILRKSL
                     QQLESAGLIQHDKTGRRVSPSGISYMDGLADRIAKEGFAQVQK"
BASE COUNT          789 a         1518 c         1240 g         1055 t
ORIGIN      
        1 cacccgggcg gaaggtatcg gcgcagatca ccccgacccg gaggcccttc cgctggaagt
       61 accgcgcaag tttcgcggtg gtggtggtct ttccgctccc ctgaagtcct gccatcagga
      121 tgacctgggg ctcaagtctc gcctcgcctg ccgagcccac cagccgtacg agctcctggt
      181 acacaatgcg gagcacgtgc tccctcactg tcatgccttt cgggggctgt tcctcgaggg
      241 accgggtctt gatggcctgg gagagctgca tcaccatctt cacgttcaca tcggcctgga
      301 tgagcgcccg ctgcaggtct tttacgagtt cttcgactgc gacccggtcg accacggtct
      361 ttcctgcgag cttcttcagg gcgtccttga gggaagtccc cagcctgtcc agcatcaggc
      421 ctcaaccccc aggagctcgt cgaccacgcg ggtgggcgag aaggggatga tatcatcgta
      481 gccctggccc accccgagga acattaccgg ctttccaatc gtgtgggcga ttgagatcgc
      541 cgcacccccc ttcgagtcca tgtcggcctt cgtgagcacg accgcatccg ccccgactgt
      601 cttctcgaac tcgaatgccc tcaccacggc atcgttgcct gcaacggcct cgtccacgta
      661 cacgaccagg tccggcttca tcactctccg tatcttctcc agctggttca tgaggtttgc
      721 gcggttgtgg aaccttcctg cagtgtcggc aaggacagca tccacccggt gcgcttctgc
      781 gtactggacc gtgtcgtaga ggactgccga agggtcggcg cccgcctggt gctggatcac
      841 cttgatccca atccttcctg cgtgcacgtc gatctgctcg atagccccgg cacgaaaagt
      901 gtcgccggca ccgatgacca ccgagcagcc attcttcttc aggtacgatg ccacctttgc
      961 aaccgtggtg gtcttgccgg ttccgttcac cccggtgaag aggatcttca ccggtttttc
     1021 gtgggtcctg atatacgcaa gaagatcaaa gccttcaccg agtaccccga gtaacgctcc
     1081 cttcagcgcg gtgacgacaa tctcgtcaac ggactctccg atcttcctcc gcttcccggt
     1141 gagatccgcc cttacccgcg tgagtatttc gtcggtgacg gggagagcga catcgttctc
     1201 aaggagaatc atctccagtt cgaaaagcgg ttcttcgata tccttctcgg caatgaaaaa
     1261 ttcgcgatcg gtgatgagaa ctttgacctt cgcaatcagg gaagggtctt tcccggatac
     1321 cgcaccctcg cattcctcag ggacagccgc agtatcggcc ggagatgcag tctttgcgat
     1381 attcttctcc agcgttccct ggaccttctg gagccttgcc cgcagcccct cgaacatcac
     1441 gcacccccgg caggccgcat tatcccaatt cttcgccctg gaccggccgg gcctgctgca
     1501 tctggtactg ttggtacccg gcttcaaggc ggcgggcaat ctcattcatc tggtacctga
     1561 gctggtcgag ggtctccatg accttcttgg cagaggcgtc catctcggtg aggcgatcct
     1621 tgaggtattc caccgccgcc tccgttgatt gctctaccac gacgtcggcc ccgatgttga
     1681 ggagcactcg ttctggctcc tcaatccggg cccggacact gacgccccct ccgatctgca
     1741 ggagtacggt atcttcggac gactccttta tgcctgttat cgcatcgatc gcggccagcg
     1801 cctccaccct cccttcttca agaagggcca gctgctgtga gaagatctcg gcctgctgcc
     1861 ggtattcgtt caggtacagc tggagcgact gcagctccct cgggtcaatc ccttccacct
     1921 tactcaccac tgagaagagt aatggactgc acggtgatat aacttctttt cagcctgtgt
     1981 ttgctcccga taagggcaaa tgtccgctct tttgcctggg cagcgttcgg ggcctccacc
     2041 actttcgcgt atgggcgcca ctcgtctccc atcctgaaat cgccttttac ctcatacttt
     2101 tgatccgcca ttctctcatc actccaaaaa tccaaatacg tcctctactc ttccaagttc
     2161 aaacccggtg gtctcgagcc ctgtcgcata gccatgccca ttcaccagga gccctgtccc
     2221 gacaaggccg acgccgcggt tgaccgatcc ggtaccgatc ggcactcccg ctgcctgctc
     2281 cagcacctgg atctcggccc tcgagctgcg gggatgcaca agcaccccct tgttggtggc
     2341 gactccggcc atcccaactg tcttgatgcc gccaagggtg agggggaaca cccggactcc
     2401 caggaactct cccacttccc ccgccatctc ccttggcata tcggggtgca ccgccgcaaa
     2461 ggagtcattt gccatgatca cgttgcctgc ggcgttcatg gtgtgctcga ggaggtatac
     2521 ctgaccgtac tcctcgagga gcgcgatctc gcgatcatgc gcaaggccgc tcaccacaaa
     2581 ccccctccgg tttccggcga ggagcgaccc gactatctcg ctcccctgga tagtggtggg
     2641 gatgatctcc acgtccagta actcgattat ggcctcctgg aactccgcag gtgcaaccgg
     2701 ggggaccacg gcgaggcctc cgattacccg ggtaaaaacg ccgatattcg agtccccggc
     2761 gatggagatc gtcctgtcca tctcactcct cggcaagttc ggcctggacc tgcccgtcat
     2821 cgaacttcat ggcccggatg cgtatgcgcg acggtggttt ctccgcccca cgatcccaga
     2881 ccctctcgtt gatggtgcgg tccagtttca cgtcctggct cttcatgtgc tttgccagga
     2941 acttcctgat atcctttatc gcggtgttgc tcctcttcca gcggggtgcc ctttttgcgt
     3001 ccctcaatgg gatgatgtag atctgttcct ccatcttgtc tgccataacc cttacacctt
     3061 cagcgtactc cttctccagt tacgcctgcg gggatgggtc gagacctggc gtttggtccg
     3121 catcatcacc catgcaggaa ccctccggtt ctgctcacac gcctttgcaa ggcgtacttt
     3181 gtatcctttg gttgatctgc tcatgtgaat tctcaccctg atattgtaat ggtccccgtg
     3241 acaagggact gctcgtggtg cagggggaag tacttccccg agtcctcgcc ccttgcccgc
     3301 acctcgtcgc gaatctccgc ttcgtagtcc ccgttctcga tgcgtcccgt ctccccgaac
     3361 gcaatctcca ggtgccgtgc agcaatgcgg tcaacttccc tgcgcgggta cccgagcagc
     3421 ggtcgcaggt agctgcagcc gctcgtgtcc tgcagggact gcacttcgtc ccgggaaagc
     3481 atggggacac gatcatcgaa ccgggtgccg tcagcgacca cccggtactc ggtggagagg
     3541 atgcggaggg cctccttgtg caccatctgg atggcgtcat tgggataccc ggaggagagg
     3601 accatctcca cggcacggcc gagaagttcc tccccgaaag tcctccggcg atagggaaac
     3661 ccgagagcaa gggccgccct ttcgacggca gcgaggctcc tctgatgact gaacacgaag
     3721 gtgttcagtt caaccgagta atccctggag aggaggactg ccgcaagaga gctgtccttc
     3781 ccgccgctga agaggactcc cgccttcatc ctttcctggt gatcctgaaa tccttctttg
     3841 ccggctgcaa ctgctgcagg agggatttca gctgtgtatc cgtgatcttc tgcttgagac
     3901 gaccgctctg ggcgagcatc accagctgct gctcaacggc cctggcaaaa tcaggccgtg
     3961 tcagcttgat ggtgttgagc ctctctctcg cttccggctc caggatctgc atgaggacag
     4021 cgtggatctg ggcctcaacc tgcttctggc gctccatctc ctcctgcatt acctgctggt
     4081 cgatggactg ctgctgcatc tgcgccagcc ttcttctccg gatttctgcg agttcgtcgt
     4141 cccccatggc cttcacctta cttctggacc tgggcgaatc cttcctttgc aatccggtcg
     4201 gccaggccgt ccatgtagga gatgccggaa gggctcaccc tgcgaccggt cttgtcgtgc
     4261 tggataaggc cagcgctctc gagctgctgg agtgacttcc ttaagatgga cccgctcccc
     4321 ttcctgaact gggacgggcg ggaacccctg tccttcttcc ctccatagat cgaacgcatc
     4381 cgctggacgc cgattgggcc ctctatgtag accctccgga ggatggatgc aacccggacg
     4441 aaccaccact cggggtcctg ggggggcatc tccttgtgca ctcccgtctt ggcgaatgca
     4501 gcccatgccg ggggtgtgat ctccggcttc ttcttcagtt cctcggccag ggtgcggatg
     4561 aacctgtcgg ccggaacatc atagacggtt gtcatatgta ct
//
LOCUS       DAOQVJ010000056         4552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_21157_length_4552_cov_4.129197, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000056 DAOQVJ010000000
VERSION     DAOQVJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21157_length_4552_cov_4.129197"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="PKL26_02025"
     CDS             complement(<1..1592)
                     /locus_tag="PKL26_02025"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNS82503.1"
                     /translation="MKFAEFARICEEIEGISGRLEMTDTISGILPGLDSRDLPIFVHF
                     ITGKIFPDWSPLKMGIGPNLLYDAITLIVDGTRSDVIGEINRRGDVGLAVQAIIEQKK
                     VSRRSSRTATIQTSLFSQELDLFGVYQKLEKIARQGGRASQQEKLSLIQSLFQNASPL
                     EARYLARLLMEDLRIGVGEGTIRDAIARAFSVDPSNVEHAYQARNDLGEVAILARAGE
                     NALRDVRILPFHPVKMMLAQAGDIHGAIAENGAMAAEFKYDGSRFQFHKAGGESRIYS
                     RKLEDVTDALPDILSALYGATTHDVILDGEVIAVKDGKPLPFQAVLRRFRRKHGIEEM
                     QKTVNVQPFVFDILYLDGETLIDLPFRERRARLEATVSDYVTPQLVSGEPDAIERFYK
                     GSLAAGHEGLMLKVLSAPYTPGIRGKNWLKIKPSVDTLDLAVIGAEWGEGKRAHLFGS
                     FLLACQDQGELQALSKVATGFSDEQLAELYDLLRDHVLSESGKEVQFEPILVFEVGYS
                     EIQESPNYECGYALRFPRFIRVR"
     gene            1737..2930
                     /locus_tag="PKL26_02030"
     CDS             1737..2930
                     /locus_tag="PKL26_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82504.1"
                     /translation="MPEEWIPDALVEQKMGQTKREMMEYVEKNSEKIDSNIPIFFGHV
                     VSILEKNYPDIPDQAYDRFIDDVTVKVLEESRHSTDIEYIEKLFRHATRVKRKYTGRA
                     IFDIVLGIKLIDMGKYSDAIEVLKKYRTVDAMVCTAIAFSYYELSSLELPPDKKPDKA
                     TTSAMALSAREQMIELARLRPPVNRLRFPQVVQELRVNKVFWFMIRLAIDWFPNEPEY
                     LKIGLEKAKKDGNREMRGELLKIASERYYDDMFFLRDLYHFKLEGRDAGGATAVVRQM
                     MQQHPDELEPIYYGLQLSIISSQESAYQKFRSTAISKKMPAHIILMLDLSFMIITGRK
                     ADAEVMLEDTKKKLPALGYYLGVLEYLMGDIFSEDEKRSRTAKKLFLDSIDMYSLKLL
                     KMKEG"
     gene            complement(3205..3993)
                     /locus_tag="PKL26_02035"
     CDS             complement(3205..3993)
                     /locus_tag="PKL26_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS82505.1"
                     /translation="MRCIALASGSKGNCLYIEGSSSSLLIDAGLSRREILSRLHSVGG
                     STEHLSAILVTHEHIDHIASVEALARHLSIPVYATGGTLLDLLDSRPVRRNPIRAVRV
                     DYGERFTIGDFSVEAFEVSHDAREPCGFLVTEGDLTVGCCTDTGMVNDHMMDCFCQCD
                     ALVLESNHCPEMLKNGPYPEVLKRRIRSRRGHLSNPDAARCVNELCTRVHMIMLAHLS
                     EMNNTPEKALSTARQGAGLFSTDIEITVATNPGTGQGWPHGIRL"
     gene            complement(4176..>4552)
                     /locus_tag="PKL26_02040"
     CDS             complement(4176..>4552)
                     /locus_tag="PKL26_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNS82506.1"
                     /translation="GVENIFDGVIAENTGGLATIDIGNTCIVAICPYPEGTRVTSYMR
                     PEDVIFLIEKDGKSTARNEIEGTISRVTPMGPSVKVRMDGGIRVVAVITRRSYDELGL
                     SPGAHVYASFKASAIHVVKNEP"
BASE COUNT         1019 a         1271 c         1278 g          984 t
ORIGIN      
        1 cggacgcgga tgaagcgagg gaaccggagt gcgtatccgc actcgtagtt ggggctttcc
       61 tggatctccg agtaccctac ctcgaatacc aggattggct cgaactgcac ttcctttccg
      121 gactctgaga gaacgtggtc ccggaggaga tcatacagct ccgcgagctg ctcatccgag
      181 aacccggttg cgaccttgct cagtgcctgg agttccccct ggtcctggca tgcgagcagg
      241 aatgaaccaa agaggtgcgc tcttttccct tctccccatt ccgccccgat gactgcgagg
      301 tccagggtat ccacggaggg cttgatcttc agccagttct ttccccgtat tcccggggta
      361 tacggtgcag agagcacctt cagcatcagt ccttcatggc cagcagcgag gcttcctttg
      421 tagaatctct cgattgcatc aggttcgcca ctcaccagct ggggcgtgac atagtcactg
      481 accgtcgcct cgagccgcgc tctccgctct ctgaagggga ggtcgatcag ggtttcaccg
      541 tcgaggtaga ggatatcaaa gacgaacggt tgcacattca ccgttttttg catctcctcg
      601 atcccgtgct tccgacggaa ccttcgcagc acggcctgga acgggagggg ttttccgtct
      661 ttgaccgcaa tcacttcgcc gtcaaggatc acgtcatggg tggtagcccc gtaaagcgcg
      721 gaaaggatat ccggaagggc gtctgtcacg tcctcgagtt tcctcgagta tatcctgctc
      781 tcgccgcccg ccttgtggaa ctggaacctg ctgccatcgt acttgaactc ggcggccatg
      841 gctccgttct cggcaatcgc accatgaatg tcccccgctt gggcaagcat catcttcacc
      901 gggtggaacg gcaggatgcg cacgtcgcgc agtgcattct caccggccct tgcaagtatt
      961 gccacctcgc caaggtcgtt cctggcctgg tacgcgtgct caacgttcga aggatcaaca
     1021 gagaatgccc gggcaatggc gtcccgtatc gtcccctccc ccacaccgat acgcaggtcc
     1081 tccatcagga gccttgcaag gtaccgtgcc tccagcggcg acgcgttctg gaagaggctc
     1141 tgtatgaggg acaatttctc ctgctgtgag gcccgtccac cctgccgcgc aatcttctcg
     1201 agtttctggt agacgccgaa gaggtcgagt tcctgggaga agagggaggt ctggatggtc
     1261 gcagtccgcg acgaccttcg cgataccttt ttctgctcga ttatcgcctg caccgcaagg
     1321 ccgacatcgc cgcggcggtt tatctcacct atcacgtcac tccttgttcc gtcgacgatg
     1381 agggtgatcg cgtcatagag gaggtttgga ccgatcccca tcttgagcgg actccagtca
     1441 gggaagatct tccctgtgat aaaatgtacg aatatcggca agtctctgga atcgagaccc
     1501 gggaggatgc cggagatcgt gtcagtcatc tcaagcctcc ctgaaatacc ctcgatctcc
     1561 tcgcaaatcc ttgcaaattc tgcaaatttc atgattgcct ggatatttag aatagtcacg
     1621 ttgggcgagg ataagggttc ccttctgcga agggtcagtt atctgaacgg ttgaataccc
     1681 gatttcggca cagtatatca aggttcttat attcttacag gtcattcctg tatacaatgc
     1741 cggaggagtg gatcccggat gccctcgtcg agcagaagat ggggcagacg aagcgggaga
     1801 tgatggagta cgtagagaag aactctgaga agatcgactc gaacatcccg atattctttg
     1861 gccacgtggt ctcgatcctc gagaagaact accctgacat ccccgaccag gcatacgacc
     1921 gtttcatcga cgacgtgacc gtaaaagtcc tcgaggagag caggcattcc accgacatcg
     1981 agtacatcga aaagcttttc cgacacgcaa cccgtgtcaa gaggaagtac acggggaggg
     2041 caatcttcga catcgtcctt ggaataaaac tgatcgacat ggggaagtac tcggatgcaa
     2101 tcgaggtgct caagaagtac cgaacggtcg atgcgatggt atgcaccgcg atcgccttct
     2161 cctattacga actctcctcc ctcgagctcc ctccggacaa gaaaccggac aaggccacta
     2221 cgagtgccat ggcgctatcc gcacgggagc agatgatcga gcttgcccgg ctccggccgc
     2281 cggtcaaccg cctcaggttc ccgcaggttg tgcaggagct acgcgtgaac aaggtcttct
     2341 ggttcatgat caggcttgcc atcgactggt tcccaaacga gccggagtac ctgaagatcg
     2401 gcctcgaaaa ggcgaagaag gatggaaaca gggagatgag gggcgagctc ctgaagattg
     2461 catcggaacg gtactacgac gacatgttct tcctccgcga cctgtatcac ttcaagctcg
     2521 aggggagaga tgccgggggc gcgaccgctg ttgtaaggca gatgatgcag cagcatccag
     2581 acgagctcga gccgatctat tacggcctgc agctctccat catctcatcc caggagtctg
     2641 cgtaccagaa gttccggagc accgcgatct caaaaaagat gccggcccac atcatcctca
     2701 tgctggacct ctcgttcatg atcatcaccg gcaggaaggc cgatgcggag gtgatgcttg
     2761 aagataccaa gaagaagctc ccggcactcg ggtattacct gggggtcctc gaatacctca
     2821 tgggggatat cttctcagag gatgagaaac gctccaggac tgcaaagaag ctgttccttg
     2881 attctatcga catgtacagc ctcaagctgc tgaagatgaa ggaaggatga agaatttctc
     2941 cccggattcg gaaaaagggc caagaaaaag actttttttt aaaaccatga tgtcgcacca
     3001 aaaaaatcac tctcattccc tttcagagca gggcattacg cccattcctc catggcgcgg
     3061 gagttcagaa ttcccatgga aggaaacaaa gaaaccaaac ggatattttg gctcaacata
     3121 tgcctgcgta ggtatcgtta cgctctcatg tgaagggggg agaatcggtc ctccgggcag
     3181 tttttgaatt cttccaggaa aaattcacaa tctgatgccg tggggccagc cctgcccggt
     3241 gccgggattc gtggcaacgg tgatctcgat gtcggttgag aagagcccgg ctccctgcct
     3301 ggccgtggaa agtgcctttt ccggggtgtt gttcatctcg ctcaagtgcg cgagcatgat
     3361 catgtgtacc cgcgtgcaga gctcgttcac gcagcgggcg gcatcggggt tggagaggtg
     3421 accccttcgt gaacggatcc gacgcttcag cacctcgggg tatggcccat tcttcagcat
     3481 ctcggggcag tggttgctct caaggacgag tgcatcgcac tggcagaagc agtccatcat
     3541 atggtcattc accatcccgg tgtcagtgca gcacccaacc gtgagatccc cttcggtgac
     3601 caggaacccg cagggttccc tcgcatcgtg ggagacctcg aaggcctcca cagagaaatc
     3661 cccgatagta aaccgctccc cgtaatcgac ccgcaccgcc ctgatgggat ttctccggac
     3721 aggccgtgaa tcaaggagat caaggagggt cccgccggtc gcataaaccg ggatggagag
     3781 gtggcgggca agtgcctcta ctgatgcgat atggtcgata tgctcgtgtg tcacgaggat
     3841 cgccgagaga tgctccgtgg atccgcctac ggaatgcagg cgggagagga tctcgcgcct
     3901 ggagagccct gcatcaatga ggagcgagga tgaactccct tcgatataga ggcagttccc
     3961 tttgctgccg cttgcaaggg cgatgcaccg cattgcatca tgtatacgcg ggcatgcgag
     4021 aaatgggtgc cggtcaatgc aatgaggtgc gttccactag ggaggcagaa ggagatccgg
     4081 tagatatgaa gggtcagagt tatgcagggt agcgggtatg actggtgttc agtgaggctg
     4141 cctgaacacc aaccttttcc ggcaagccat cggatttacg gttcgttctt cactacatgg
     4201 atggcgctgg ccttgaagga ggcatagaca tgggcgcctg gcgagaggcc aagctcatcg
     4261 tatgaacgac gggtgatgac tgcaacaacc ctgatgccgc catccatcct gaccttcacg
     4321 ctcggcccca tgggagtcac acgagagatg gttccctcga tctcgttgcg cgcggtgctt
     4381 tttccatcct tctcaatgag gaaaatgaca tcttcagggc gcatatacga ggtgacacgt
     4441 gtcccttcag gataggggca tatggccaca atacacgtgt ttccaatgtc gatcgtggcc
     4501 aggccacctg tattctcagc gataacacca tcaaagatat tctcgacacc ga
//
LOCUS       DAOQVJ010000057         4508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_21435_length_4508_cov_3.628340, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000057 DAOQVJ010000000
VERSION     DAOQVJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21435_length_4508_cov_3.628340"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(570..1403)
                     /gene="truA"
                     /locus_tag="PKL26_02045"
     CDS             complement(570..1403)
                     /gene="truA"
                     /locus_tag="PKL26_02045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNS82507.1"
                     /translation="MEEVHTGNAAQVRLAFRCGYLGEGFFGSQVQPVLRTVEGEFIRA
                     CLNLGLFSSHRGARFLAAGRTDRGVHARGQVFAFSTVHPDRAVSALNRRLPPDFWTTG
                     YAVVEPGFHPRHHAVSRTYRYYFGEPDLDAEAMDRAARIFCGVHDFSLFARVEGRDPV
                     RKVLTSRVFLDQGLCVYEVSAESFLWHMVRYMASTLLMAGLGADGDEVILSRLAGDGR
                     ARLSPAPPEGLVLWDVAYAFPFVPVSAGEKALALLESRGAYHRCMGRVIDTLRDTTND
                     M"
     gene            complement(1367..2035)
                     /locus_tag="PKL26_02050"
     CDS             complement(1367..2035)
                     /locus_tag="PKL26_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNS82508.1"
                     /translation="MLNCDLHIHTSFSRDGESSVEEIVRRAELVGLDAIAITDHDTVE
                     GALHALTVPTRLVIIPGIEVSTRQGHLLVLGVTTHIPRGMEVTETIRRARELGGLVIL
                     PHPYHIWRHGVARRMRSPLSEVDAIEVFNSRYIVGSANRKAARIARLLKKPCVGGSDA
                     HNARFVGYGRTLVDAAPDTASILDAIRAGRTVATGRMTPLRTYTRQSLSNTWKKFTRG
                     MQPR"
     gene            2078..3490
                     /locus_tag="PKL26_02055"
     CDS             2078..3490
                     /locus_tag="PKL26_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNS82509.1"
                     /translation="MRILLPTGRATLEVVREAAAGIDARVVVTGEIASFLSPARLADL
                     LSEEKYDMAIVSGMCTASFSSVEAESGVPVYLGPRHAADLGLVLPLLPGVRLSRTIPA
                     DEFLAGERRKKAVLRIASTEQESDFIYSVRGVKIGGGSRMKVLAEIMDAHRVPDLRAR
                     VEALFSLGADIVDLGFGFDATFDQVRSAFSALEDIDGPLSVDTQDPALIAAALPRADM
                     VLSLQEENIPLVGSLVAGAGAVAVVVPGKAGLRENLAMARDAGVECLVADPLLPPAGS
                     GLVDSLARFRGWDVPLFFGAGNVVELLDADSVGANALLCAMAAEIGASVIFTSEHSDK
                     TQGAVREMRRATEMMVLSRDRPYPKDLGLDLLVIKEKRRRREPPLEYRTFVQAGPVPA
                     EIEYDPHGNFRIGIEGAEVVAVIRDRAYRGTSWAEVFFAILAGGEVSLLDHAAYLGKE
                     LYKAELAIRFGRSFEQDGPF"
     gene            3591..3962
                     /locus_tag="PKL26_02060"
     CDS             3591..3962
                     /locus_tag="PKL26_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HNS82510.1"
                     /translation="MQIRGISTDLLDLLLEVGKDRHPYEFAGVIMEEDGLLQELFLLP
                     GTVSSEESASLFYDMMPLDTHVAGSAHSHPNGVLFPSNADLSFFPRVGRYHLIVGFPY
                     SREDWRCYRANGEPVALEVIG"
     gene            3959..4438
                     /locus_tag="PKL26_02065"
     CDS             3959..4438
                     /locus_tag="PKL26_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HNS82511.1"
                     /translation="MRRVVATGTFDILHPGHLYYLEESRRLGDELYVIVARDRNVRHK
                     PKPVLPEEQRARMVAALKPVDHAVLGDLHDMFRPIEEIRPAVITFGFNQHFDEENIRG
                     ELGKRGLSVELARIGSYGGDRYCSSSEIVRQVLARFSSGKDEMSEREHKEPSYLGSH"
BASE COUNT          882 a         1332 c         1431 g          863 t
ORIGIN      
        1 gggcccttaa ttactcatat gagtaaaaat acatgaatgt gttgttgagg gcaaatccgg
       61 cggaaagggg gatcagtcgt cgtagatctc gcaggaatac ccgtgcgtgg cgaggaccga
      121 actgcagtgc cgggcaaact ggtcttcagg cagagtggag gagggcatca acacctcctc
      181 caagacccct gtgagtaacg ggtcactggt agagagggag catcttcctt cacgtttccc
      241 ggcttcagac ccattgctgt tgagaatgcg caatggtgca gcacctgtac atccagcata
      301 tcaatggacg ggaggagtgg accctgcagg aaaaagaccc gtcggccgcc ctcaggagga
      361 aaaggcccca ccctttttcg aagaagggag gagaacgtga gagaaagagg tcgcgaagtc
      421 cagggctgac ctgcacaggc aggctctcct cagtcacccc aacgcggaga atgtgggaga
      481 acagcagata cgcttctctt gataaccatg tggcgtccaa ggctgatgca gcatcttctg
      541 catcaggccg ggggaactct ggcatctcgt cacatgtcgt tcgtggtatc ccgcagcgta
      601 tcaatcaccc gccccatgca cctgtgatat gcaccccggc tctccagcag cgccagcgct
      661 ttttcgcccg cggaaacggg gacaaacggg aacgcatacg ccacatccca gagcaccagg
      721 ccttcggggg gggcagggga aagcctggca cgcccatccc ccgcaagtct ggacaggatg
      781 acctcatcgc catcagcccc cagccctgcc atgaggagcg tagaggccat gtacctgacc
      841 atgtgccaca ggaagctctc ggcagagacc tcatacacgc agagcccctg gtccaggaat
      901 accctggagg tcaggacctt cctcaccggg tcccttcctt caaccctggc aaagagcgag
      961 aagtcatgga ccccgcaaaa gatccgcgct gcacggtcca tcgcttcggc gtcgaggtca
     1021 ggttccccga aataatagcg gtacgtgcgg ctgacggcat ggtgccgggg gtgaaacccg
     1081 ggttccacca ctgcataccc ggtggtccag aaatccggtg ggagccgccg gttcaatgcg
     1141 gagacggccc ggtcgggatg aacggtcgag aaagcgaaga cctggcccct cgcgtggaca
     1201 ccgcggtcgg tcctcccggc tgcaaggaac cgcgccccgc ggtggctgga gaagaggccg
     1261 aggttcaggc acgcccggat gaactcgccc tccactgtcc ggagcacagg ctgcacctgg
     1321 gatccaaaga acccttctcc caggtacccg cagcggaagg ccagccttac ctgggctgca
     1381 ttccccgtgt gaacttcttc catgtattgc tcagggactg gcgggtgtag gtgcgaaggg
     1441 gggtcatcct ccccgtggcg accgtccttc ccgcccggat cgcatccagg atcgacgcag
     1501 tgtccggtgc tgcgtccacg agcgtcctcc cgtatcccac gaatctcgcg ttgtgggcat
     1561 cggaaccgcc gacacagggt tttttcagca ggcgggcgat ccgtgcggcc ttgcggtttg
     1621 ccgaacctac gatgtacctg ctgttgaaga cctcgatcgc gtccacctct gagaggggcg
     1681 accgcatcct ccttgcgacg ccgtggcgcc atatatggta agggtgcggc agtatcacga
     1741 ggccgccaag ctcccgggcc ctcctgatcg tctcggtcac ctccattccg cgggggatgt
     1801 gggtggtaac ccccagcacc aggaggtgcc cctggcgggt ggagacctcg atccccggga
     1861 taatgacaag tctcgtcgga acagtcaggg catggagggc gccttccacc gtgtcgtggt
     1921 cggtgatggc gatcgcatcg agccccacga gctctgctct cctgacgatc tcttccacgc
     1981 tgctctcccc gtcgcgggaa aaacttgtgt gaatgtgcag atcgcagttc agcatctgat
     2041 catacctttt ttgtcctccg tgatattaag aagacttatg cgaatcctcc tccccaccgg
     2101 gagagcgacc ctggaggtgg tcagagaggc cgctgccgga atcgatgccc gcgtggtggt
     2161 caccggggag atcgcctcgt ttctctcgcc ggccaggctt gccgatctcc tctcggagga
     2221 gaagtatgac atggcaatcg tctccgggat gtgcacggcg tcgttttcat cggtcgaggc
     2281 cgaatcgggg gtcccggtct acctcggccc gaggcatgcc gcagatctcg gcctcgtcct
     2341 ccctcttctc ccgggtgtca ggctctcgag gacgataccg gcggacgagt ttttggccgg
     2401 agagaggagg aagaaagcgg ttttgcgcat cgcatctaca gagcaggagt cggacttcat
     2461 ctattccgtc aggggagtga agatcggggg aggttcccgc atgaaggtcc ttgccgagat
     2521 catggacgca caccgggtac cggacctccg cgcgagggtg gaggctctct tctctctggg
     2581 agccgatatt gttgacctcg ggttcgggtt cgacgccaca tttgaccagg tccgctctgc
     2641 cttctctgcc ctggaagata tcgacgggcc actttccgtg gacacgcagg acccggcgct
     2701 gattgccgca gcgttgccga gggccgacat ggtgctctcc ctgcaggagg agaacatccc
     2761 ccttgtgggg tctcttgttg ccggggcggg agcagtggcc gtggtggtac ccgggaaggc
     2821 cggccttcgg gagaacctgg caatggcacg ggacgcggga gtcgagtgcc ttgtcgcaga
     2881 ccccctcctc cccccggcag gatcaggcct ggtcgactcc ctggcccggt tcaggggatg
     2941 ggacgtcccc ctcttctttg gggcgggcaa cgtcgtcgag ctcctggatg ccgattctgt
     3001 cggcgcaaac gcgctcctgt gcgcgatggc cgcagaaatc ggtgcctcgg tgatcttcac
     3061 gagcgagcat agcgacaaga cgcagggggc agtccgggag atgcggaggg cgaccgagat
     3121 gatggtcctc tccagggacc ggccctatcc gaaggatctc ggcctcgacc tgctggtgat
     3181 caaggagaag cggaggcgga gagagccccc gctcgaatac cggacctttg tgcaggcagg
     3241 cccggtgcct gcagagatcg agtacgaccc gcatgggaac tttcgcatcg ggatagaggg
     3301 ggcagaggtt gtcgcagtca taagagaccg ggcataccgg ggaacatcct gggcagaagt
     3361 ctttttcgcc atccttgcag gaggggaggt ctcactcctc gatcatgccg cgtacctggg
     3421 aaaggaactc tacaaggcag agttggcgat ccggttcggc cggagtttcg agcaggacgg
     3481 ccccttctga caggggacaa cggaggtgtg gcaggcagaa cccggggatt ttgcccttgc
     3541 ttcctgcacc atggtttaat gccgtcttct tgccaattat gatgaagcat atgcagatac
     3601 ggggcatcag cacggacctc ctggacctcc tcctggaagt ggggaaggat cgtcatcctt
     3661 acgagtttgc aggtgtgatt atggaggagg atggcctgct gcaggagctc ttcctgttac
     3721 cagggaccgt gagcagcgag gagagcgcca gccttttcta tgacatgatg cctctcgata
     3781 cccatgttgc ggggagcgcc cacagccatc ccaacggcgt gctcttcccc tcgaacgccg
     3841 atctctcctt ttttccaagg gtcgggcgat accacctcat cgtggggttc ccgtacagcc
     3901 gggaggactg gcggtgctac cgtgcaaacg gggaaccggt tgcgctggag gtgatcgggt
     3961 gaggcgcgtg gtggcgaccg gcaccttcga tatcctccac cccgggcacc tgtactacct
     4021 cgaggagtcc aggaggctcg gggacgaact gtacgtgatc gtcgccaggg accgaaacgt
     4081 ccgccataag ccaaagcccg tgctgcctga agagcagagg gcgaggatgg tcgctgccct
     4141 caagccggtt gatcacgcgg ttctcggcga tcttcatgac atgttcaggc cgatcgagga
     4201 gatacgcccc gcggtcatca ctttcgggtt caaccagcac tttgacgagg agaatatacg
     4261 gggtgagctt ggcaaacggg gactctctgt cgaactggcc cggataggga gctacggcgg
     4321 ggaccgctat tgcagctcga gtgaaattgt ccggcaggtg ctggcccgat tctcctccgg
     4381 gaaggatgag atgtctgaac gggaacacaa agagccatct tacctgggca gtcactgaag
     4441 ggttcggtat ttatcccttc cggcacggtt ccggagaggg aaccatatta acgtggaagg
     4501 tgaaataa
//
LOCUS       DAOQVJ010000058         4491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_21545_length_4491_cov_4.172227, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000058 DAOQVJ010000000
VERSION     DAOQVJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21545_length_4491_cov_4.172227"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /gene="dnaK"
                     /locus_tag="PKL26_02070"
     CDS             <1..1484
                     /gene="dnaK"
                     /locus_tag="PKL26_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNS82512.1"
                     /translation="AYFNDAQRQATKDAGQIAGLEVLRIINEPTASALAYGIDKEQDV
                     TVLVYDLGGGTFDVSILTLGDGVFEVKSTSGNNHLGGDDFDNRIMDYLIEEFQKKEGI
                     NLRTDPIAIQRLRDAAENAKVELSQKVKTNINLPYITTGPSGPKFLDIDLTRAKLEQL
                     IGDLVESTVGPVKQALSDAKLTPDQIDHVLLVGGSTRVPLVQETVRKILGKEPDKGIN
                     PDECVGLGAAIQGAVLSGETKDIVLLDVTPLTLGIETLGGIATKLIERNTTIPTRKGQ
                     IFSTAADGQTSVEIHVVQGERALAKDNFTLGRFQLTGIPPAPRGIPQIEVTFDIDANG
                     IIHVSAKDLGTGNEQAITIKGDRKLTEDDIKRMTEDAKKFEAEDAKKKEEIELRNNAD
                     IAIFSAEKMLKEQGNNIEPADREKVETGVADLRKVLEADNSDEIRAKMDTLTEAVYTI
                     TTKIYQKAQAERQAQQAESGDKGGSTTPGDENVVDADFKVKDE"
     gene            1490..2620
                     /gene="dnaJ"
                     /locus_tag="PKL26_02075"
     CDS             1490..2620
                     /gene="dnaJ"
                     /locus_tag="PKL26_02075"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNS82513.1"
                     /translation="MSEGDYYEILGVSRNAEEKEIKKAYRNLARKYHPDVCKEPGAEE
                     QFKKINEAYSVLSDSQKRAQYDHLGHDTYTSASKGSYSGTGGFHGGGFSADFSGFGDI
                     FDFFGGGFSGSRQTGPQSGSDLLMKMQISLEDAVFGADREIEVNHTEACTHCNGTGSE
                     SKKLVKCPRCGGSGQMRQMSQTIFGQFVRMSTCTECRGRGRIPEKRCTVCKGSGHTQV
                     HRKVTVHIPAGIDSGMRLRMEGYGEAGDYGAPNGDLFIEVYVRPHDRFRRTGDHLETE
                     VEVSPAQAVVGSIVDVETIEKRTVELTIPAGIQHNTALKIPGEGVRRRGKPGDLLVRV
                     RVVIPKQPKAEIRSLYEKILELEGHRMADSKKGFFSGFMGKK"
     gene            2753..3715
                     /locus_tag="PKL26_02080"
     CDS             2753..3715
                     /locus_tag="PKL26_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS82514.1"
                     /translation="MHLLVELAGDHPQLPFAELDILGPVLERGTQVALVDCQHAEEIP
                     RLALAHSAMEHLGSCSAEKGAFSRMLKDLAISSPRPFAGRVKKVYGNTMDASQAELER
                     LIGSLVQGKVDLQNPAEEFRAIVAGSRCYLGRVLWRSDPRRFGDRRPGDRPFFHPGVM
                     MPRMVRALMNISCARQGERLLDPFSGTGGMIMEAALLGIIGIGSDIDPEMVRGSRMNA
                     REGVILRADGRRLPFRDESFDAVVTDLPYGQSVSIRARSLDSLYHDALGEISRVVKPG
                     RRVVLVTHRDIRMRAGNFMRVEGCYSQRVHRSLTRNILVLVRDG"
     gene            3790..>4491
                     /locus_tag="PKL26_02085"
     CDS             3790..>4491
                     /locus_tag="PKL26_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNS82515.1"
                     /translation="MHYEMKEGEVRGGGVIWTCSRTDPGVRDRNEDASGTFTLPHREG
                     ALYLLAVADGLGGHPAGEVASAIAISALRSAVSSTVEALEGMDVSSLQAILATGFSHA
                     NREVIRYAATTPCCLGMGTTLVAALMNGEGAGVVGNVGDSRAYLFGEGTVRITRDHSR
                     VQEMVDQGLISQEQAGNHPLRHIVTRIIGRTDDIPDFFPFRLKDDLLVLCSDGLVDGV
                     SEGELQSLAGRHGSPD"
BASE COUNT         1044 a         1211 c         1411 g          825 t
ORIGIN      
        1 cggcatattt caacgatgcc cagcgccagg ccaccaagga tgcaggccag atcgcgggtc
       61 tcgaggttct gaggatcatc aatgaaccaa cggccagtgc gctcgcgtac ggtattgaca
      121 aggagcagga cgtcactgtg ctcgtctatg acctcggcgg cgggacattc gatgtctcga
      181 tcctgaccct cggagatggg gtgttcgagg taaaatccac atcgggaaac aatcacctgg
      241 gaggggacga cttcgacaac aggatcatgg actacctcat cgaggagttc cagaagaagg
      301 agggcatcaa tctccgcacg gacccgatcg cgatacagag actgagagat gctgccgaga
      361 acgccaaggt cgagctctcc cagaaggtga agacaaacat caacctgccg tatatcacta
      421 cgggaccaag cggccccaag ttcctggata tcgacctgac cagggcaaag ctcgaacagt
      481 tgattggcga cctggtggag tcgacagtgg ggcctgtcaa gcaggcacta agcgacgcaa
      541 aactcacccc cgaccagatc gaccatgttc tcctcgtcgg cgggtctacg agggtaccgc
      601 tcgtccagga gaccgtcagg aagatactcg gcaaagaacc tgacaaaggt atcaatcctg
      661 acgaatgtgt agggctcggc gctgccatcc agggggcggt cctctctggg gagacgaagg
      721 atatcgtgct cctggatgtc acaccactca ccctggggat cgagaccctc ggcgggatcg
      781 cgaccaagct gatcgaaaga aataccacca tcccaacccg caagggccag attttctcca
      841 ctgcggctga tggccagacg agcgtggaga tccacgtcgt gcagggcgag cgtgcgcttg
      901 cgaaggataa tttcaccctc ggccggttcc agctgaccgg catccccccg gcaccgcggg
      961 gaatcccgca gatcgaggtc actttcgata tcgatgccaa cggtatcatc catgtctctg
     1021 caaaagacct cggcacaggg aacgagcagg cgatcaccat caagggtgac aggaaactca
     1081 ccgaggacga tatcaagaga atgaccgagg atgcaaagaa gttcgaggca gaagacgcca
     1141 agaaaaaaga agagatcgaa cttcggaata atgccgatat cgcaattttc tctgccgaaa
     1201 agatgctgaa agagcagggg aacaatatcg aacccgctga cagggagaaa gttgagacag
     1261 gggttgccga tctccggaaa gtgctcgagg cagacaacag cgatgagatc cgtgcgaaga
     1321 tggatacgct gaccgaagca gtgtatacca tcaccaccaa gatctaccag aaagcccagg
     1381 cggaacgcca ggcacagcag gcggaatccg gggataaggg gggctccacc accccgggtg
     1441 acgaaaacgt cgtcgatgcc gatttcaagg ttaaagacga gtgagcccga tgagcgaggg
     1501 tgattactac gagatcctgg gtgtctccag gaacgcggaa gagaaggaga ttaagaaggc
     1561 ctaccgcaac ctggcccgga agtatcaccc cgatgtctgc aaggagcctg gcgcagagga
     1621 gcagttcaag aagatcaacg aggcttacag tgtcctctcc gattcacaga agcgtgcgca
     1681 gtacgaccat cttggtcacg acacctatac cagcgcctcg aaagggtcgt acagcgggac
     1741 agggggtttt catggcggag gcttcagcgc cgacttcagc gggttcggcg acatattcga
     1801 tttttttggg ggtgggttct ccgggtcgcg gcagacgggc ccgcagtcgg gttccgacct
     1861 cctgatgaag atgcagatct cgctcgagga tgcagtcttt ggtgcagacc gcgagatcga
     1921 ggtgaaccat accgaggcgt gcacccactg taacggcacc gggagcgaga gcaagaagct
     1981 tgtaaaatgc ccaaggtgcg ggggttcggg ccagatgagg cagatgagcc agacaatctt
     2041 tggccagttc gtgaggatga gcacctgcac cgagtgccgc ggacggggaa ggatcccgga
     2101 gaagcggtgc acggtatgca agggatcggg gcatacccag gtgcacagga aggtcacggt
     2161 gcacatcccg gccggcatcg acagcgggat gcggctccgg atggaagggt acggggaggc
     2221 cggggactac ggcgcaccga atggcgacct cttcatcgag gtctacgtgc ggccgcatga
     2281 ccgtttccgc cggaccggtg accaccttga gacagaagtg gaggtaagcc cggcacaggc
     2341 agtagttggc tccattgtgg acgtggagac aatcgagaag cggaccgtgg aactgaccat
     2401 ccctgcgggg atccagcaca ataccgccct gaagatcccg ggagaagggg tcaggcgcag
     2461 ggggaagccc ggcgacctcc ttgtaagggt cagggtggtg atcccgaaac agccaaaagc
     2521 tgagatccga agcctctacg agaagatact ggaactggag gggcaccgga tggctgattc
     2581 aaagaaaggg ttcttttcgg ggttcatggg gaagaaataa taaattcctc tcgttttcac
     2641 agtatatccc tcttccaatg gtaatttcac tcaatcccgg ggattgagat catcactctc
     2701 atgacctcgt ttatcccctg gtttttttgc cttcactgtc ctattggtgg gcgtgcacct
     2761 actcgttgag ctcgccggag accacccgca gttgccattc gccgagctcg acattcttgg
     2821 acccgtgctc gagagaggca cccaggtcgc cctggtcgac tgccagcatg cagaggagat
     2881 cccacgtctc gcacttgcac attcggccat ggagcacctg ggctcctgtt ccgctgaaaa
     2941 gggcgcattt tcacgcatgt tgaaagacct cgcgatatcg agtccccggc cgttcgcagg
     3001 aagggtaaag aaggtctacg gcaacaccat ggatgcgtcc caggcggagc tcgaacggct
     3061 catcgggtcg ctcgtgcagg gaaaggtgga cctgcagaac ccggccgagg aattccgggc
     3121 aatcgtggca gggtccaggt gctaccttgg ccgcgtcctg tggaggagcg acccgcgacg
     3181 atttggagac cgccgtccag gtgacaggcc cttcttccat ccgggggtca tgatgccccg
     3241 gatggtgcgt gcgctgatga atatctcctg tgcccggcaa ggcgaaaggc tccttgaccc
     3301 tttctcgggc accggcggga tgatcatgga ggcggccctt ctcggcataa ttgggatcgg
     3361 aagcgacatt gaccctgaaa tggtcagggg gagcaggatg aatgcacggg aaggcgtgat
     3421 tctccgtgcc gacggcagga gactgccgtt ccgtgacgaa tcgttcgatg cggtggtcac
     3481 cgatctcccc tatgggcagt ctgtctcaat cagggcacgc agccttgact cgctttatca
     3541 cgatgccctt ggggagatca gccgcgtggt taaaccgggc cgccgggtgg tcctggtcac
     3601 tcaccgggac atccgcatgc gtgcaggcaa tttcatgagg gtggagggat gctactccca
     3661 gagggtccac aggagcctga cgaggaacat actcgtcctt gtaagagatg ggtgaatata
     3721 atggcatacc gaaggactga caccattcaa atggaatgac tgcgaaaaat tgagggggat
     3781 cttctcagaa tgcattatga gatgaaagag ggagaagtcc gtggcggtgg ggtgatatgg
     3841 acctgctcac gcactgatcc gggagtccgt gaccggaacg aggatgctag cggcacattc
     3901 accctccctc acagggaagg agcactctat ctccttgcag tcgctgacgg actgggaggt
     3961 caccctgcag gggaagtggc aagcgccatc gctatctccg ccctgcggag tgccgtgtct
     4021 tcgacggttg aggcactcga gggcatggac gtctcttccc tgcaggcgat ccttgccacg
     4081 gggttttcac acgcgaaccg cgaggtgata cgctatgcag cgacgactcc ctgttgcctt
     4141 ggaatgggga ccaccctcgt tgcggctctc atgaacgggg aaggagctgg cgtcgtgggg
     4201 aacgtgggag acagccgcgc ctacctcttt ggagaaggta ccgtgaggat cacccgggac
     4261 cactcaaggg tgcaggagat ggtggaccag gggctgatat cgcaggagca ggctgggaat
     4321 catccgttga ggcatattgt cacaaggatt atcgggagaa cggatgatat ccctgacttt
     4381 ttccctttcc gcctcaagga cgatctcctt gtcctctgct cggatggact cgtcgatggt
     4441 gtcagcgagg gggagctgca gtccctggct ggcaggcatg ggtcccctga t
//
LOCUS       DAOQVJ010000059         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_21674_length_4475_cov_4.436878, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000059 DAOQVJ010000000
VERSION     DAOQVJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21674_length_4475_cov_4.436878"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKL26_02090"
     CDS             complement(<1..416)
                     /locus_tag="PKL26_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS82516.1"
                     /translation="MILSVDELRFFYRNQEVLADIAFSIDEGEIVAILGPNGVGKTTL
                     LKCLNRILTPRGGAVLLDGDDLSRLDLMEIARKVGYVPQRVETGRLTAFDAVLLGRRP
                     HIGWDVTGKDLKVVDAVFRRLHMDHLRLSYIDEMSGG"
     gene            complement(413..1483)
                     /locus_tag="PKL26_02095"
     CDS             complement(413..1483)
                     /locus_tag="PKL26_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNS82517.1"
                     /translation="MHFADGEIPADYLGYVRNKVFWILGGLLVLFVLLVYSISVGAVE
                     IPLHEVIATLLGQNVSHKWDAIIWNIRLPQALTAIAAGVGLSVAGVAMQSILRNPLGS
                     PFTLGISNAGAFGAAVSVIVLGTGTMQSRVADAVTINNPYLTTMMAFLFCLLATAVIL
                     LISRIRGASPEVMVLAGVALSSLFTAGTMFLQFFSNDAQLAAVVFWTFGDVSRANWPE
                     LALIAVIVAAATVYFIANRWNYNAIDAGDETAKGLGVNVERVRLVGMVVAALVSAVIV
                     AFLGVIGFVGLVCPHMARRIIGDDQRYLIAGSCVMGGILLLASDTVARLIVAPYVLPV
                     AVLTAFMGAPVFIYLLLRGYRR"
     gene            complement(1534..2625)
                     /locus_tag="PKL26_02100"
     CDS             complement(1534..2625)
                     /locus_tag="PKL26_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HNS82518.1"
                     /translation="MSNSAPATEVPSATGKAATLAVKDGFGRDVTVPVPLERVVCSGS
                     GGLRYLVYLQAQDLAVGVDDIEKKEQAIEGRPYALAWGSQFKALPLIGEYRGKDDPEK
                     IIAISPEAVFKTGSTGTAYATSAADADKLQEKTGIPVVAFPYGSLRNDAEKAEMYAGL
                     RVMGKALGKEDRAEEIIAYVEATMKDLESRTAGIAAADQKTAYVGGVSSAGAHGTIST
                     EPAYPPFLWVNAKNVAAGMGTAHADIAKEALVEWDPEFIFVDVGTLQLDNQGAVGELK
                     SDPALRGLSAVKEGRVYGVLPYNFYNVNYESVLADAYFVGKVLYPDRFADIDPAQKAD
                     EIYTFFVGKPVFSQMNSQYGGFGFSQIPV"
     assembly_gap    2794..2893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2894..>4475)
                     /locus_tag="PKL26_02105"
     CDS             complement(<2894..>4475)
                     /locus_tag="PKL26_02105"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS82519.1"
                     /translation="LDRELGIQPIQHDPAAFDRLLEGAERLISGIYGTRIPEGMHVFG
                     RIPAGPKRARFIAPILNHDGHLHRLLAGMMGLDIRISPSETALLRVLDGYSEDLVSRL
                     LEGEPPSQAASGVLGDRLIRIDEEGLASLNGEVQEISRALDRSDEIGSLISGTKGSYI
                     PPGPSGLLSRGKTGILPTGRNFYSLDPSSVPTEAAWEVGTRLAEILVERYRQEHGVYP
                     ENVALLWMASDIMWADGEQCAQALALIGAEPVREHGRLKGVRIIPLERLGRPRIDLTI
                     RVSGILRDCFFGCVEFLDDAIRAVAALDEPSEWNYLRKHSVDNETGPRIFGAPRGTYG
                     MGVNLAVYASAWNNVSDLADVFIYWNGYSYGRGVFGEKSTEHLVSQLRTVDVTFNKTA
                     TDEYDLLGCCCYFGSHGGMTAAAREVSGKEVSAYYGDTRNTSRVEVRTLAEELRRVVR
                     TKLLNPQYIDCLKEHGYAGAAELSKRAGRVFGWDATTGEVDDRIFDDIARTFLLDAKN
                     REFFRDHNIFAMEEMARRL"
BASE COUNT          848 a         1403 c         1294 g          830 t
ORIGIN      
        1 ccgccgctca tctcgtcgat gtaggagagc cggaggtggt ccatatggag ccgcctgaac
       61 accgcgtcca cgacttttaa gtccttcccg gtcacgtccc agccaatgtg agggcgcctt
      121 ccgaggagga ccgcgtcaaa cgccgtgagc ctcccggtct cgacccgctg gggaacatac
      181 cccaccttcc gggcgatctc catcaggtcg agccgcgaga ggtcgtcgcc gtcgaggagg
      241 acggcgcccc cccggggcgt gaggatgcgg tttaagcact tgaggagcgt ggtcttaccg
      301 acgccgtttg gcccgagaat cgccacgatc tccccttcat caatggaaaa cgcgatatcc
      361 gcgagcactt cttggttccg gtagaagaac cggagctcgt caacagagag gatcatcttc
      421 ggtaccccct gagcagcagg tagataaaga ctggcgcacc catgaacgcg gtcaggaccg
      481 cgaccggcag cacgtaaggt gcgacgatca gccgtgccac cgtgtccgat gcaagcagga
      541 ggattccccc catcacgcag gaccctgcga tgaggtaccg ctggtcgtcg ccgatgatcc
      601 tccgtgccat gtgggggcac acgagcccca cgaacccgat cacgccgagg aatgcgacga
      661 tcaccgctga gacgagcgcg gcaacgacca tccccaccag gcgcacccgc tcgacgttca
      721 ccccgagtcc cttcgcggtc tcgtcgcccg catctattgc gttgtagttc caccggttcg
      781 cgatgaaata gaccgtcgcc gccgcgacaa tgaccgcgat cagcgcaagc tccggccagt
      841 tcgcgcggga gacatcgccg aacgtccaga agacgacggc tgcaagctgg gcgtcgtttg
      901 agaagaactg caggaacatg gtcccggcgg taaagagcga ggagagcgca acgcctgcaa
      961 gtaccatcac ttcaggcgac gccccccgga tccgcgagat gagcaggatc acagcggtcg
     1021 cgagcaggca gaagaggaac gccatcatgg tcgtcaggta ggggttgttg atggtgaccg
     1081 catccgcgac cctcgactgc atcgtgccgg tcccgagcac gatgaccgag actgccgccc
     1141 cgaatgcccc cgcgttcgag atgccgagag tgaacggcga gcccaggggg ttccggagga
     1201 tcgactgcat cgcaacgcct gcaaccgaga gccccacgcc tgccgcgatc gccgtcagcg
     1261 cctggggaag ccggatgttc cagatgatcg cgtcccactt gtgggagacg ttctggccga
     1321 gaagcgtggc gatgacctca tggaggggga tctcgactgc gccgaccgag attgagtaca
     1381 cgaggaggac aaagaggacg aggagtcctc cgagtatcca gaacaccttg ttccggacat
     1441 agccgaggta gtcggccggg atctctccgt cagcaaagtg catcctgcct caccaccctc
     1501 cctgtgctgc agcggctgta gtcatgtccg catctagacc gggatctgcg agaacccgaa
     1561 ccctccgtac tggctgttca tctgcgaaaa gaccggtttt ccgacaaaga atgtgtagat
     1621 ctcgtctgcc ttctgcgccg ggtcgatgtc tgcaaaccgg tcggggtaga ggaccttgcc
     1681 gacaaagtag gcgtccgcga gcaccgactc gtagttcacg ttgtagaagt tgtagggaag
     1741 caccccatag accctgccct cttttaccgc ggagagcccc cgcagcgccg ggtcgctctt
     1801 cagctcgccg actgcaccct ggttatccag ctggagggtc ccgacgtcga cgaagatgaa
     1861 ttcagggtcc cactctacga gggcttcctt ggcgatgtcg gcatgggcgg tccccatccc
     1921 cgcggcgaca ttctttgcgt tcacccagag gaacggcgga taggcaggct cggtggagat
     1981 ggttccgtgc gccccggcgg aactgacgcc tcccacgtac gcggtcttct ggtcagcggc
     2041 tgcaataccg gcggtccttg actcgaggtc cttcatcgtc gcctctacat acgcgatgat
     2101 ctcttcagca cggtcttcct tgccgagcgc ttttcccatc acgcggagac ctgcatacat
     2161 ctcggccttc tcagcgtcat tccggagcga tccatagggg aacgccacga cggggatgcc
     2221 ggtcttctcc tggagcttat cggcatctgc cgcgctcgtg gcatacgcag tcccggtcga
     2281 tccggtcttg aagacagcct cggggcttat cgcgatgatc ttctcggggt cgtccttgcc
     2341 cctgtactcg ccgatgaggg gaagcgcctt gaactgtgac ccccacgcga gggcataggg
     2401 acgcccttcg atggcctgct ccttcttctc gatgtcgtca accccgaccg cgaggtcctg
     2461 ggcctgcagg tatacaaggt agcggaggcc tcccgacccc gagcagacca cgcgctcgag
     2521 tggtaccggg accgtcacgt ccctcccgaa cccgtccttc acggcaagcg tggccgcctt
     2581 gcctgtcgcc gaaggtactt cggttgcagg ggcgctattg ctcatgcagc ccgctatcgc
     2641 gagcattgca cagagcacga gtgccattga ggtgagaata aggaactttt tcatactttt
     2701 attatttttc atcatattat ttcaatatag tcaaagtatc ttcatattat agataagaga
     2761 ataaattcct ctgtatgaaa ttatttgcag agtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnagccttc gtgccatctc ctccattgca aagatgttgt ggtcccggaa
     2941 gaactcgcga ttttttgcgt ccagtaaaaa ggtccgtgca atatcatcga agatcctgtc
     3001 gtcgacctcc ccggtcgtcg cgtcccatcc aaagacccgc ccggcacgct tggagagctc
     3061 cgcggcgccc gcatacccgt gctccttcag gcagtcgatg tactgggggt tcagaagctt
     3121 tgtcctgacc acgcggcgaa gctcctctgc gagggtgcgg acctccaccc ttgaggtgtt
     3181 ccgtgtatcc ccgtaatagg cggacacttc cttgcctgat acctcccgtg ccgcggcggt
     3241 catgccgcca tgggagccaa aataacagca gcacccgagg aggtcgtatt cgtcggtcgc
     3301 agtcttgttg aaggtcacat caacggtccg caactgggag accaggtgtt ccgtcgactt
     3361 ctccccaaag actcccctgc cgtaggagta cccgttccag tagatgaaga catccgcaag
     3421 gtcgctcaca ttattccagg ccgatgcgta gactgcaagg ttgaccccca tcccgtacgt
     3481 ccctctgggt gcgccgaaga tccttgggcc ggtctcattg tccaccgaat gctttcgcag
     3541 gtagttccac tccgacggct cgtcgagtgc ggccacagcc ctgatcgcat catccaggaa
     3601 ctcgacacac ccgaagaagc agtcccgtaa aatgccgctc accctgatcg tgagatcgat
     3661 tctcggcctg ccgagccgct cgagcgggat gatgcggaca cccttcagcc tcccgtgctc
     3721 ccggacgggt tcggcgccga tcagggccag ggcctgtgca cactgctccc cgtcggccca
     3781 catgatatcc gaggccatcc agaggagggc cacgttctcg gggtagaccc cgtgctcctg
     3841 gcgataccgc tcgaccagga tctccgcaag gcgggtccct acctcccatg ccgcctcggt
     3901 cgggaccgat gaagggtcaa gggaatagaa gttcctcccg gtggggagga tcccggtctt
     3961 tcccctggag aggaggccgg aaggccccgg cgggatgtag ctgcccttcg ttccactgat
     4021 caggctcccg atctcatccg agcgatcgag ggcacgggag atctcctgca cctccccgtt
     4081 cagggaggcg agtccctctt catcgattct gatcaggcgg tccccgagca ccccagaggc
     4141 cgcctgcgag ggtggctctc cttcgaggag gcgggagacc aggtcctcgg agtagccgtc
     4201 gagcacccga aggagtgcag tctcggaagg ggagatccgg atatcgagcc ccatcatgcc
     4261 ggcaagcaac cggtggagat gcccgtcgtg gttcaggatt ggcgcgatga accgggcccg
     4321 cttcggaccg gccgggatgc gcccgaacac gtgcatcccc tccgggatgc gggtgccgta
     4381 aatacccgag atcagccgct cggcgccttc cagcagacga tcaaacgctg cggggtcatg
     4441 ctgtatgggc tggataccca gctcacgatc aagcc
//
LOCUS       DAOQVJ010000060         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_21793_length_4457_cov_4.144707, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000060 DAOQVJ010000000
VERSION     DAOQVJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21793_length_4457_cov_4.144707"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PKL26_02110"
     CDS             <1..727
                     /locus_tag="PKL26_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS82520.1"
                     /translation="THLFLVSKPSSSFGILPVKGEDFLPVFEETLAVARMVSETGITV
                     YPVLGVHPAEITRLMESFPLSRVVEIMKEGIALAAGFVIDGAAVGLKSGRPHYEVSPE
                     VWDAANDVLSYSFQKAGEAGCAIQVHAESGPCADMREMARAVGLSPDRVVKHYAIPET
                     TLVPSLLATHPDIPALAMEGRAFMMESDYMDELSRPGAVIGPRSVPRVTTRLLSTGQI
                     NEDAAHRIHVSTPSQVYGVDISL"
     gene            777..1070
                     /locus_tag="PKL26_02115"
     CDS             777..1070
                     /locus_tag="PKL26_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HNS82521.1"
                     /translation="MYLKVHRSPAGDEVVAVCDRELMNTTLSGNEVEIRITGSFYGTE
                     LATPEEVSSALSRAENANLMGERTVALAVEMGLLSRSACLMFGDVPHAQLFRI"
     gene            1075..2115
                     /locus_tag="PKL26_02120"
     CDS             1075..2115
                     /locus_tag="PKL26_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HNS82522.1"
                     /translation="MHIRESICPLCGNPSPSGEVCGQCRALSTRWLECPPRVSTVLCP
                     TCGARKDAGGWSDLQLEREELARQLIKRAIWLHPDLRDVSMNIALDEISTNRSRAECR
                     ISGILFGAPVEGSCSLEVELRREQCDRCSRISGSYYEGVVQVRATGRRPSQRELEETA
                     RIACAVEASMTESGERLSFISRLEEHRDGIDIIVGSQRLGSEIATAISRDLGGRFTTH
                     PKLVGEKAGRQVFRITYSLRLPRYSRGDIVLVSGRHLEVLGVDGKNIRCLDHASGMVR
                     SVPERQVERKAGHKGDALEWMVAYRDGDLLGVLDPRTGVTKEVRIPRGKETSAGTTVR
                     ILEDGDLLVLLE"
     gene            2121..2990
                     /locus_tag="PKL26_02125"
     CDS             2121..2990
                     /locus_tag="PKL26_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS82523.1"
                     /translation="MRVREIPVNELCSLKGADWVDRNRRTYLDGNIAFVPVREGCPCT
                     STLPQRRPYQGRGYFMLGDVAVFHGRRPGEDEVRNLMEWKRPAGVLWVQSCKGTMRTP
                     QTEVLAGTVQEVRHRECGITYWLDPASIMFSMGNRDEKERIRSLVHPGERIADMYAGI
                     GYFTLPSALAGARVHAMEIRPLAFSYLERNIIGNGVAHLVTAELGDCRDCLLGTYDRI
                     IMGHFGSIDRLTDALAHSGPGTVLHVHSAGALKDKIQAILEETGVTAAIAIHKVKKLA
                     PHTWHYVQDVTLE"
     gene            2987..4159
                     /gene="coaBC"
                     /locus_tag="PKL26_02130"
     CDS             2987..4159
                     /gene="coaBC"
                     /locus_tag="PKL26_02130"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNS82524.1"
                     /translation="MSGINTLEGKEIVLAVTGSIAAVDTVRLAHALRRRGARVQAVMS
                     SAACGILHPAALTYATGRPAITGITGMVEHVAWCGEGGMADLLLVAPATANTLCKIAA
                     GIDDTPVTTFATTAVGKGMPIVVAPAMHESMFRHPGVAGCLRTLEGWGITVVGPRIEE
                     GKAKIADIGDIVLHCERELMGKPLLGHHVLVTSGPCRERVDDVRVLTTRSTGLMGRAL
                     ALQAFRLGARVTVVHADRFPAVENVRTTDAAGMLKEVTRICREDPVDLFISAAAVSDF
                     CPEPCPGKIPSGNTVTLTLNPLPKIIDDVMDRFHVPTIAFKVGDDAVTRGRTMLGDSI
                     GLVVANDPNVMGEVDTRVTMLGQGGFVREASGSKEAVAAEILDCAMEIFRFLKKER"
     gene            complement(4264..>4457)
                     /locus_tag="PKL26_02135"
     CDS             complement(4264..>4457)
                     /locus_tag="PKL26_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82525.1"
                     /translation="TIFLSVALGVAEACGAKLRERYRVMSAGYLLKSAANPLPGPLPR
                     NAIACGQGCAKTFRNLPAI"
BASE COUNT          897 a         1302 c         1427 g          831 t
ORIGIN      
        1 aacgcacctc ttcctggtca gcaagccctc ctcctccttt ggcatcctgc ccgtgaaggg
       61 agaggacttc ctcccggtct ttgaggagac cctggcggtc gcgcggatgg tctcagagac
      121 tggcatcacg gtctaccccg ttctcggcgt tcatcccgcc gagatcacgc gcctgatgga
      181 atcgtttcca ctgtcccgcg tggtggagat aatgaaggag ggtattgcac ttgcggccgg
      241 gttcgtcatt gacggcgcgg cggtcggctt aaaaagcgga agacctcact acgaggtctc
      301 ccctgaggtc tgggatgcgg caaacgacgt cctctcatac tccttccaga aggccggcga
      361 ggccgggtgc gcgatccagg tgcatgccga gagcggtccc tgtgccgaca tgagggagat
      421 ggcgcgggct gtcgggctct cccccgatcg ggtggtaaag cactacgcaa tccccgaaac
      481 aacccttgtt ccttcgctgc ttgccaccca tcccgatatc ccggcactcg cgatggaggg
      541 gcgggcgttc atgatggaga gcgattatat ggacgaactc tcccgtcccg gggcggtcat
      601 cgggccacgc tcggtcccgc gtgtcaccac caggcttctc tcgaccgggc agatcaacga
      661 ggatgcagcg caccggatcc acgtatccac gccatcgcag gtctatggcg tggatatctc
      721 gctctgatga cctccagttg cagtattaat acgcatccct ccctacatgt ggtatcatgt
      781 atctgaaggt tcaccgctct cctgccgggg acgaggtggt ggcagtatgc gaccgggagt
      841 tgatgaatac cacgctctct ggaaacgaag tagagattcg catcaccggg tcattttatg
      901 ggacagaact tgcaaccccg gaagaggtga gttcggcact ctcccgcgcc gagaacgcaa
      961 acctcatggg tgagcgaacc gtggccctgg cggtggagat ggggctcctc tcccgctcgg
     1021 cgtgcctgat gttcggtgat gtgccccacg cgcaattgtt caggatttaa aaatatgcat
     1081 atcagggaga gcatctgccc cctctgcgga aatccctcac ccagcggaga ggtctgcggg
     1141 cagtgcaggg ctttgagtac ccgctggctg gaatgccccc cgcgggtatc cacggtgctc
     1201 tgccctacct gcggagcgag gaaagacgca ggaggatggt ctgatctcca actcgagagg
     1261 gaggaacttg cccgccagct tatcaagagg gcaatatggc tccatcctga cctccgcgat
     1321 gtgtcgatga acatcgcact cgacgaaata agcaccaacc gcagcagggc cgaatgcagg
     1381 atctccggga tactctttgg tgcgccggtc gaggggtcct gctcacttga ggtggaattg
     1441 aggcgggagc agtgcgaccg ctgcagcagg atctcgggga gttactacga gggagtggta
     1501 caggtacggg ctacgggaag gagaccttcc cagagggaac tggaagagac cgcccggatc
     1561 gcctgcgcgg tcgaggcgtc gatgacggaa tccggggaac gcctctcgtt catctcccgc
     1621 ctcgaggagc acagggatgg catcgatatc attgtcggct cccagcggct gggctccgag
     1681 atagccactg caatctcccg ggaccttggc ggcaggttca ccacccaccc aaagctcgtg
     1741 ggggagaagg cgggcaggca ggtcttccgg atcacctact ccctccggct tccccgctat
     1801 tcccggggcg atattgtcct ggtctctggc aggcacctcg aggtgctcgg ggtggacgga
     1861 aagaacatcc ggtgccttga ccatgcatcc ggcatggtac ggtctgttcc cgaacggcag
     1921 gttgaacgaa aggctggcca caagggagac gctcttgagt ggatggtcgc ttatcgcgac
     1981 ggcgacctcc tgggagtcct tgacccgagg accggagtca caaaggaagt gcggatcccg
     2041 aggggaaagg agacatctgc cggaaccacg gtacgcatcc tcgaagacgg ggaccttctg
     2101 gtcctgctgg agtgagcctg atgagagtca gggaaatacc cgtcaatgaa ctctgcagcc
     2161 tgaaaggcgc cgactgggtc gaccggaaca ggcggacata tctcgacggc aacattgcct
     2221 ttgtccctgt cagggaaggc tgcccgtgta cctccactct gccccagagg agaccatacc
     2281 agggcagggg gtatttcatg ctcggggacg tggcggtctt ccacgggagg cggcccggag
     2341 aggacgaggt ccggaacctg atggagtgga agaggcctgc aggagtcctg tgggtccagt
     2401 cctgcaaggg aacgatgaga accccacaaa cggaggtcct tgcagggact gtgcaggagg
     2461 tccgccacag ggagtgcggg attacgtact ggctggaccc cgcatccatc atgttctcca
     2521 tggggaaccg ggacgagaag gagcggatac gctcccttgt ccatcccggc gagcggattg
     2581 ccgacatgta tgcagggatc gggtatttta ccctcccttc ggccctggca ggtgccaggg
     2641 tccatgcaat ggagatacgg cccctcgcgt tctcttacct tgagagaaac atcatcggga
     2701 acggtgtcgc ccacctcgta accgcagagc ttggcgactg cagggactgc cttttgggga
     2761 cctatgacag gatcatcatg ggacatttcg ggtccatcga ccgcctcacg gatgccctgg
     2821 cccattcagg gccgggaact gtcctgcacg tccattcggc aggtgcgctg aaggataaga
     2881 tccaggcgat ccttgaagag acaggtgtca ctgccgcaat agcgatccat aaggtaaaga
     2941 aactggcgcc gcatacctgg cattacgtgc aggacgtgac cctggaatga gcggaatcaa
     3001 cacgctggaa ggaaaggaga tcgtgctcgc ggtgacaggg agcattgctg cagtggacac
     3061 cgtgcgcctc gcccatgccc tccggaggag gggtgcgcgg gtgcaggcag tgatgagcag
     3121 cgcagcctgc ggcatcctcc accccgctgc cctgacctat gcgaccggga gacccgcgat
     3181 caccgggatt accgggatgg tcgagcacgt cgcctggtgc ggtgagggtg gcatggcaga
     3241 cctgctcctg gttgccccgg cgaccgcaaa caccctctgc aagatcgccg caggaatcga
     3301 cgataccccg gttaccacgt ttgccacgac cgcggtggga aaggggatgc caatcgtcgt
     3361 ggccccggcc atgcacgaga gcatgttcag gcacccgggg gttgcaggct gcctgcgcac
     3421 cctggaaggg tgggggataa cggtggtggg cccccgtatc gaggaaggga aggcaaagat
     3481 tgccgatatt ggggatattg tactccattg cgagcgggaa ttgatgggaa agcccctcct
     3541 cgggcaccac gtcctcgtca ccagcgggcc ctgcagggag agggtggatg atgtgcgggt
     3601 cctcaccacg cgctccaccg ggctgatggg gcgggcgctt gcactgcagg cgttccggct
     3661 tggggcacgg gtgacggtcg tccacgctga ccggttcccc gcggtggaga acgtgcgcac
     3721 cacggacgcg gccgggatgc tcaaagaagt cacccggata tgcagggagg accccgtcga
     3781 tctcttcatc tctgctgcag ctgtctcgga tttctgcccc gagccctgcc cgggaaagat
     3841 cccgagcgga aataccgtca ccctcaccct caatcccctc ccgaagatca tcgatgacgt
     3901 aatggaccgg ttccatgtgc cgacgatcgc cttcaaggtc ggggatgacg ctgtgacaag
     3961 gggcaggacc atgctcgggg acagcatcgg gctcgtggtg gcaaacgacc ccaacgtcat
     4021 gggtgaggtt gacacccgcg tgaccatgct cgggcaggga ggattcgtgc gcgaagcatc
     4081 cggaagcaaa gaggccgttg ccgccgagat actcgattgc gcgatggaga tcttccggtt
     4141 tttgaagaaa gaaagataat cccctctttt tcctgcagga caagagtatt attatacaag
     4201 actttgcttc agggggctta ccctgagcac tgtatcagtc ccctggtggg gagcgggcaa
     4261 gatctaaatg gcaggcaggt ttctgaaggt ttttgcgcaa ccctgcccgc aggctatcgc
     4321 gtttcggggg aggggaccgg ggaggggatt cgctgcactc tttaagagat agcccgcact
     4381 catcacacga taacgctctc ggagcttcgc cccgcacgct tccgcaaccc caagcgccac
     4441 gctcaagaaa atcgtgt
//
LOCUS       DAOQVJ010000061         4410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22108_length_4410_cov_4.429851, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000061 DAOQVJ010000000
VERSION     DAOQVJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22108_length_4410_cov_4.429851"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKL26_02140"
     CDS             complement(<1..369)
                     /locus_tag="PKL26_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNS82526.1"
                     /translation="MINAEKFISNAVEEIRREAGERRVVMALSGGVDSSVCAALASRA
                     IGDRLEPIYVDTGLMRKGETERISQVFGKLHLHVVPAEEEFLGALRGVCDPEEKRKVI
                     GERFIRVFEREARRTGARALL"
     gene            complement(383..1969)
                     /locus_tag="PKL26_02145"
     CDS             complement(383..1969)
                     /locus_tag="PKL26_02145"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNS82527.1"
                     /translation="MKYIFITGGVMSGLGKGITAASVGRILKNRGYGITAVKIDPYLN
                     IDAGTMNPGQHGEVFVLSDGSEVDLDLGNYERFLDIDLSSAHNITTGKVYRTVIEKER
                     RGDFLGETVQIIPHITDEIKACIRAAAEMEIPGQKKADICLVEIGGTVGDIESMPFLE
                     AVRQMRGDLTLQDTVIIHVTLIPEDTMGDLKTKPTQHSVKALRELGLHADIIVGRSER
                     PIGSHTKRKISAFCDLPLKGVISAATVPDIYQVPLELEKEGLGDVLSEHLGLEKRRAD
                     TSWYHLVSREYTDRVSVGIVSKYGIEDVYLSIKEALKHAGRALSTEVRITWLDAERYE
                     QKQLAEFDGILVPGGFGKRGIEGKVDAIQWARTNRTPFLGLCLGFQMAVVEYSRHVLG
                     WEDATSAEIGEGTHVIAILPEQHEVKELGGTMRLGDSPITLKEGTLAMQVYGQSAITE
                     RHRHRYEVNPRFIPDLEKAGLVFSGLFQNRMEICEIPDHPFFMATQFHPEFRSRPTRP
                     SPPFVGFVKACSSRNGRRAR"
     gene            complement(2174..2653)
                     /locus_tag="PKL26_02150"
     CDS             complement(2174..2653)
                     /locus_tag="PKL26_02150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNS82528.1"
                     /translation="MAGPMGRAMFFMVQGPPPGMNYLARIREMGSKANPFFGLMGIEV
                     EHAGEGEAIISMTVRPDMLNGEGWLQGGVFTALADEAMVLAIYSLIGPDDRIATITES
                     TTFIGGSREGCLYALGKVVKKGRRVVFAEGEVRDGARAGPLLTRSTAAFAVRAGHRT"
     gene            2686..2913
                     /locus_tag="PKL26_02155"
     CDS             2686..2913
                     /locus_tag="PKL26_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82529.1"
                     /translation="MDPEEKAMLADLIWLNAVIATELIQITENTSAILRKEPPPQSCL
                     EDHRRLKSIATTIAERYHPGSGLKEHLVGHQ"
     gene            2900..3679
                     /locus_tag="PKL26_02160"
     CDS             2900..3679
                     /locus_tag="PKL26_02160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS82530.1"
                     /translation="MVTSEPDAGTVRPPQGENGIQANDPCPADACSVDPPYTLIIPAY
                     NEETRIPRLLSQLGGARGEIICICEGDDHTPALVSEFAQNHRGVDIRCDRRAVRLGKG
                     GAILEGMRQARAPLVGYMDADASTSFSQMLALFALLDGADAVIGSRWIEGSVLVRGQG
                     LFRRLESRAFNGIIRTLFGLPFKDTQCGAKVFKKAAIDAVIEQMVSTGFEFDVELLWR
                     IRNCGFRIREHPITWNDMGDSRVRGNDVVRMLASLLRLRFS"
     gene            3689..>4410
                     /locus_tag="PKL26_02165"
     CDS             3689..>4410
                     /locus_tag="PKL26_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82531.1"
                     /translation="MPLQTADERKRLAFQRFEQGDFPGSYALCRDLVAGSADPAVAIL
                     CATNLFQMGRLEEAEAYFRDLANSIPESSHVHSYLGRILERKGDDQALSEFAQAVALD
                     PGNLEALRSYASFLLATGDPGRAVPVLRQLAARSRRPDDSLLLVRALLLNGMPREALD
                     VRRGPLCRGIPDDDEYIQILDACGEYAETATAARAAYERTGNLEYARSALSALARSNP
                     GGAPGEYQKFLSEKDDPGIRFD"
BASE COUNT          874 a         1407 c         1187 g          942 t
ORIGIN      
        1 aaggagcgcc cgtgcaccag tcctcctcgc ctcccgttcg aagacacgga taaatctctc
       61 accgataacc tttcgtttct cctcgggatc gcaaaccccc ctgagtgccc ccaggaactc
      121 ctcttccgca ggcactacgt gcaggtgaag ttttccaaag acctgggaga tcctctcggt
      181 ctcccccttc cgcatcagac ctgtatccac gtaaatgggc tcgagcctgt ccccgattgc
      241 ccgcgatgcc agtgccgcac agacagagct gtcgacaccg ccagagaggg ccatcaccac
      301 tctccgttca cctgcctcgc gccggatctc ctcgacggca ttcgaaatga atttctcagc
      361 gttgatcatg ttctgtcgca cctcacctcg ctcttctccc gttccggctg gagcatgcct
      421 tcacgaaacc gacgaacggt ggagacggcc tcgtcggcct cgacctgaac tcggggtgga
      481 actgcgtggc catgaagaat ggatgatccg gtatctcaca gatctccatc cggttctgga
      541 acaaccccga gaagaccagt ccagccttct ccaggtcagg aataaatctt ggattgacct
      601 cgtaccggtg cctgtgccgt tccgtaatcg cgctctgccc gtatacctgc atggcaagcg
      661 ttccttcttt taaggtgatc ggagaatccc cgagccgcat cgtcccgccc agttctttta
      721 cttcatgctg ttcaggcagg atggcgatca catgtgtacc ttcgccaatc tcggcactcg
      781 tggcgtcctc ccacccaagc acatggcggg aatattcaac caccgccatc tggaacccaa
      841 ggcacaggcc aaggaaaggt gttctgttgg tcctggccca ttggatggca tccactttgc
      901 cctctattcc ccgttttcca aatcctcccg ggacaaggat cccgtcgaat tccgcaagtt
      961 gcttctgctc gtaccgttcc gcgtcgagcc atgtgatcct cacctcagtg gaaagcgccc
     1021 tccccgcgtg tttcagcgcc tccttgatgc tcagatacac gtcttcaatc ccgtacttgc
     1081 tcacaatccc tacactcacc cgatcggtgt attccctgct gaccaggtga taccaggaag
     1141 tgtcggccct ccgcttctcc aggccaagat gttcggagag gacgtccccg agaccctcct
     1201 tctccagctc aagcgggacc tgatagatat ccgggacagt cgccgcacta atgacaccct
     1261 tgagaggaag atcgcagaac gccgagatct tgcgcttggt gtgggacccg atggggcgtt
     1321 cgctccggcc cacaatgatg tctgcatgga gaccgagttc gcgaagggcc ttgaccgagt
     1381 gctgggtggg ctttgtcttc aggtccccca tggtatcctc gggaataagc gtgacgtgaa
     1441 tgatcactgt gtcctgcagg gtgagatcgc cccgcatctg cctgacagcc tcaagaaacg
     1501 gcatactctc tatatccccg actgtgcctc caatctcgac aaggcaaata tccgctttct
     1561 tctgccccgg tatctccatc tcagcagcgg cacggatgca tgccttgatc tcgtcggtga
     1621 tgtgcgggat gatctggacc gtctcgccaa gaaagtcgcc gcgccgctct ttctcgatca
     1681 cggtgcgata caccttcccg gtggtgatat tatgcgccga tgagaggtcg atatccagga
     1741 agcgttcata gttgccgagg tcaaggtcaa cctcgcttcc gtccgaaagg acaaacacct
     1801 ctccgtgctg cccggggttc atcgtcccgg cgtcgatatt caggtaggga tctatcttga
     1861 cggcggtgat cccatacccc ctgttcttca ggatacgccc caccgatgcc gcggtgatgc
     1921 ctttcccgag cccgctcata accccgcccg tgatgaagat gtatttcaca ggaatttccc
     1981 ctctgaccct ctcatgcttg ccattcccgg catcaggaag gccgcaatgc ggcggcgcct
     2041 cccgcggtcc ccggtgctca tgatctcttc ctctctttta tgcctccaag agagatagtt
     2101 gtatcccgtc ccatggcctg cagggggatg gaacgctctc cgatcccctc cgggttgccg
     2161 gaatacccgt atctcacgtg cgatggccgg ctctcaccgc gaacgctgcg gtgctccggg
     2221 tgaggagcgg gccggctctt gcaccatccc tgacctcccc ctcggcaaat accactctcc
     2281 tccctttttt caccactttt ccgagcgcat acaggcagcc ctcccgggaa ccgccgatga
     2341 acgtggtaga ctcggtgatg gtcgcgatac ggtcatcggg gccaatgagg gaatagattg
     2401 ccagcaccat tgcctcgtcc gcgagcgccg tgaatactcc cccctggagc catccctctc
     2461 cgttcagcat gtccggtcgg actgtcatgg agataatcgc ctcgccctcc cccgcatgct
     2521 ccacttctat ccccatcaga ccgaagaatg ggtttgcctt gcttcccatc tcccggatac
     2581 gtgcaaggta gttcatacct ggaggtgggc cctgcaccat gaagaacatg gccctgccca
     2641 ttggacccgc catgattatt cgtatgcccg ccaatacact cttccatgga cccggaagag
     2701 aaggcaatgc ttgccgacct tatttggttg aacgcagtaa ttgcaacgga gctcatccag
     2761 atcaccgaga acacctctgc catcctgaga aaggagcctc ccccacagtc atgcctggag
     2821 gaccacagga ggctcaaaag cattgcgacc acgatagcag agcggtatca ccccgggtcg
     2881 ggactgaagg agcaccttgt tggtcaccag tgagcctgat gcggggacgg tgcggccccc
     2941 ccagggggag aacggtatcc aggcgaacga tccgtgcccg gcagatgcat gcagcgttga
     3001 ccccccctac acgctgatca tccctgcgta caacgaggag actcgtatcc cacgactcct
     3061 ctcgcaactt ggaggagcac gcggcgagat catctgcatc tgcgagggcg atgaccacac
     3121 acctgcgctc gtatcggagt tcgcgcaaaa ccatcgcggc gtcgacatcc ggtgcgaccg
     3181 cagggccgtg aggcttggaa aaggtggagc gatcctcgag gggatgcggc aggcccgcgc
     3241 cccccttgtg ggttacatgg acgcggatgc atccacctca ttttcccaga tgctcgcgct
     3301 cttcgcactt cttgacgggg ctgacgcggt gatagggtca cggtggatcg agggatcggt
     3361 cctcgtccgc gggcagggtc tcttcaggag gctcgagagc agggcattca acgggatcat
     3421 ccgcacactc ttcggcctcc ctttcaaaga tacgcagtgc ggggcaaaag tatttaaaaa
     3481 ggcagccatt gacgcagtaa ttgaacagat ggtctccacc ggttttgaat ttgacgtaga
     3541 acttctctgg cgaatccgga attgcgggtt tcgaatcagg gagcacccca tcacatggaa
     3601 tgacatgggc gactcgcggg tgaggggaaa tgacgtggtg cgaatgctcg caagccttct
     3661 gcggctgcgt ttttcatagt aagatcacat gcccctacag actgccgacg agagaaaacg
     3721 acttgcattc cagcgctttg agcaggggga cttcccaggt tcgtacgccc tctgccgcga
     3781 ccttgtcgcc gggtctgcgg accctgcggt ggccatactc tgtgccacca atctcttcca
     3841 aatgggacgt ctcgaggagg ccgaggcata tttccgggat ctcgccaatt ccatcccgga
     3901 gtcttcccac gtgcacagct acctcgggcg gatactcgag cgaaaaggcg atgaccaggc
     3961 cctgtccgag tttgcccagg ctgtggcgct cgaccctgga aacctcgagg cgttgagaag
     4021 ttatgcgtcc tttctccttg caacaggaga ccccgggagg gccgtgccgg tgctgcggca
     4081 gctcgcggcg cggtcccgcc gtcccgacga ctccctgctt cttgtaaggg cgcttctgct
     4141 gaacgggatg ccacgcgagg cactggacgt ccgcagaggg cctttgtgcc gcggcatccc
     4201 tgatgacgac gagtatatcc agatcctgga cgcatgcgga gagtatgccg agacggccac
     4261 tgccgcacgt gccgcatatg aaagaacagg taatctcgaa tatgcaagga gtgccctctc
     4321 agcgctcgcc cgttccaatc ctggcggtgc acctggagaa taccagaaat ttctctctga
     4381 aaaggacgat ccgggaatca ggttcgatta
//
LOCUS       DAOQVJ010000062         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22198_length_4398_cov_3.481464, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000062 DAOQVJ010000000
VERSION     DAOQVJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_4398_cov_3.481464"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKL26_02170"
     CDS             complement(<1..376)
                     /locus_tag="PKL26_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82532.1"
                     /translation="MGLMDLVWPVFFPALGAIAALVNIWWKRHSGASALRVFLMWQLA
                     IGLGLSYIYAGFGHLVFPDQVAESIGWTPGSPFQREVGMWDFSIGIVGLLCLKFRSIW
                     FWTAIVIAFGIFSIGAGIGHVYE"
     gene            complement(539..1894)
                     /gene="gltA"
                     /locus_tag="PKL26_02175"
     CDS             complement(539..1894)
                     /gene="gltA"
                     /locus_tag="PKL26_02175"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNS82533.1"
                     /translation="MGERPVRERLRDFLEVDCGISPDEAVAEAERCLQCKKPSCVKGC
                     PVEIDIPAFIARIADRDFSAAARIIKDDNLLPAVCGRVCPQEVQCEGECILGVKDSPV
                     RIGALERFAADYEREQGFVVPPLAGPTGKLVAIVGSGPAGLTAAAELARAGHSVTVFE
                     SLHEAGGVLMYGIPAFRLPKEVVQAEIDQLRKMGVDIRTNHLAGASVPVPELLAYDAV
                     FIGTGAGLPFFMNIPGEQLNGVYSANEFLTRVNLMHAERFPEYDTPVRHAGRVVVVGG
                     GNVAMDAARVARRLGARVTLVYRRREEDLPARRAEVVRAREEGVEFALCANPVRVLGT
                     NFVTGVECVRMEMCEIDESGRPEPTPVGGSNFVIDADMVIQAIGQGPNPLLIRKLGGI
                     RYGRKGNIVVDEEGRSSLDRVYAAGDVATGAATVILAMGGAKKAARAIDRMLREDRKG
                     H"
     gene            complement(1887..2735)
                     /locus_tag="PKL26_02180"
     CDS             complement(1887..2735)
                     /locus_tag="PKL26_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNS82534.1"
                     /translation="MYRVYEAGQLADRIFQIWVEAPHVARHALAGQFLVIRIDEKGER
                     IPLTVSSVEGDLVRVIFMAVGKTTNQLSDLKAGDLIRDVAGPLGRPSEMGQYGTCVVV
                     GGGAGIASCPIIARAAREAGNHVVGIIGARNAGFLILEDEMRDACHELLVATDDGSRG
                     FHGFAADVLKQVIEQQQVDCVWIIGPAIMMKVTSEVTRPKGIRTFVSLNPIMVDGTGM
                     CGSCRVQVGGETKFACVDGPEFDAHLVDFDLLMQRQRTYFDEEKVSLERYHEHACRCG
                     ESSHHG"
     gene            complement(2778..>4398)
                     /gene="arcS"
                     /locus_tag="PKL26_02185"
     CDS             complement(2778..>4398)
                     /gene="arcS"
                     /locus_tag="PKL26_02185"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HNS82535.1"
                     /translation="KRDGLARMATLIAEGKTYHLPTVLDTGSLFDGLSGSRMENVPLG
                     ADPAFVAQYLPRDRDFPAWVHPSGENIPAHGTCVMVPCWHSAFANPRRYVEWLKAMKS
                     ALPPDTAWYAPGAALPSNAAILCYSGFDILDFTAVDLRSAQGRFCIPEGEFPGDRIGS
                     GVCTCPGCRSGDLKEHNRLALVRELALAREYIACQRLRDLVESRCRMDASQVAILRHL
                     DRARGFFEKAVPIARAGTLLATTGEVLQRPEVARFSERVVGRYRPPPADVAVLLPCSA
                     RKPYSSSQSHRRFRQAIAGRALELIVTSPLGLVPRELELVYPAAHYDVPVTGYWDAEE
                     REFVGGIIGEFLEKHRFKRVIAHLEGGALAAAENGAARAGMGLEITCTGRPTSHESLA
                     FLDSSLSGENRVHHDTIRGTSSWQFDLAIDTRNLVIKGRYPWIRAFRGKTPSFSIDEA
                     TGLLRPTFEGWNMIPEGYRVIIDEFVPTGDVLAPGVVDADPHIREGDEVLVTGPCAIA
                     TGRAAMPADEMLRSWRGVAVRVRKVKTLPRT"
BASE COUNT          846 a         1460 c         1264 g          828 t
ORIGIN      
        1 gctcgtagac atgccctatg cctgccccga ttgagaatat tccaaacgcg atcacaatcg
       61 cagtccagaa ccagatagac ctgaatttca ggcagagtaa cccgacaatc ccgatcgaga
      121 agtcccacat cccgacctcg cgctggaagg ggctgccagg ggtccacccg atggattcgg
      181 caacctggtc cggaaacacc aggtgcccaa accctgcata aatataagag aggccgaggc
      241 ctatggcgag ctgccacatg aggaagacac ggagggcaga cgctcccgaa tgacgcttcc
      301 accatatatt cacgagggcg gcgattgcgc caagcgccgg gaaaaagacc ggccatacga
      361 gatccatgag tcccatggtg atcacttgaa tggagggcga taaaaaaaca ttgtatccct
      421 cctggaatca gggatatggc gcccctggat tactgggaaa aggtcgtttt cgtgactggt
      481 cccgagttct ctactgagga ccgcgttgtc gtcgtgagaa ggttcgtggc atacgagatc
      541 agtggccttt tcgatcttcc ctgagcatcc ggtcgatcgc tctcgctgcc ttcttggccc
      601 cacccatggc aaggatgacc gtcgctgcgc cggtggcaac atcccctgct gcatacaccc
      661 ggtcaagcga gctcctccct tcctcgtcga cgacaatatt tcccttcctc ccgtacctga
      721 tccccccgag cttcctgatc aggagggggt ttggtccctg gccgatcgcc tggatcacca
      781 tgtccgcatc gatcacgaag ttgctccccc caacaggagt gggttccggc cggccggatt
      841 cgtcaatctc gcacatctcc atccgcacgc actcgacacc cgtcacaaaa tttgttccga
      901 ggacgcggac ggggttcgca cagagcgcaa actccactcc ttcttccctt gcacgtacca
      961 cctctgccct gcgtgcgggg aggtcctcct ctctccgccg gtagacaagc gtcacccgtg
     1021 caccaagcct ccgcgccacc cgggctgcgt ccatggcaac gttccccccg ccgaccacca
     1081 cgacacgccc ggcgtggcgg acaggggtgt cgtattcggg aaaccgctcg gcgtgcatca
     1141 ggttcacgcg ggtcaggaac tcgtttgccg agtagacacc gttcagctgt tcaccgggga
     1201 tattcatgaa gaacggaagt cccgcgccgg ttccaatgaa gaccgcatcg tacgcgagaa
     1261 gttctggcac cgggacactc gcccctgcca ggtggttggt gcggatatcg acgcccatct
     1321 tcctgagctg gtctatctcg gcctgcacca cctcttttgg gagccggaac gccgggattc
     1381 catacatcag cacgcctccc gcctcgtgaa gcgactcaaa gaccgtcacc gagtgcccgg
     1441 cccgcgccag ctcggctgcg gcagtcagtc ctgccggacc agatcccacg atcgccacca
     1501 gttttccggt cggtccagcg agcgggggga caacgaaccc ctgctccctc tcatagtctg
     1561 cagcgaagcg ctcaagggcc ccgatgcgga ccggtgaatc cttcactccg aggatgcact
     1621 ccccctcgca ctggacttcc tgcgggcaga ccctgccaca gacggcaggg aggaggttgt
     1681 catccttgat gatccgtgca gcagcggaga agtcccggtc cgcgatgcgg gcaatgaagg
     1741 ccgggatgtc gatctccacc gggcacccct tcacgcagga gggcttcttg cactgcaggc
     1801 accgttccgc ctccgcaacc gcctcatcgg gcgagattcc gcagtccacc tctaaaaagt
     1861 cacggagtcg ttcccggacg ggccgttcac ccatggtggc tgctctcccc gcaccggcag
     1921 gcgtgctcgt ggtaccgctc gagggatact ttctcctcgt caaaataggt ccgctgccgc
     1981 tgcatcagga ggtcgaagtc gacaagatgg gcatcaaact cggggccgtc gacgcatgca
     2041 aactttgtct cccctcccac ctggacgcgg caggaaccgc acatcccggt cccgtccacc
     2101 atgatcgggt tgagggagac aaaagtcctg atccccttcg gccgggtcac ctccgaagtc
     2161 actttcatca tgatcgcagg gcctatgatc cagacacagt cgacctgctg ttgctcgatc
     2221 acctgcttca gcacgtcggc cgcaaaccca tggaacccgc ggctcccgtc gtccgtggcg
     2281 acgaggagct catggcaggc gtcgcgcatc tcgtcttcga gtatgaggaa cccggcgttc
     2341 ctggccccga tgatgccgac gacatggttt ccggcctccc gtgcagccct tgcgatgatt
     2401 gggcagcttg ctatccctgc gcctccccca accaccacgc atgtgccgta ctgtcccatc
     2461 tcgctcggtc ttcccagcgg acctgcgaca tcccgtatga gatctcctgc cttcagatca
     2521 gagagctggt tggtggtctt ccccaccgcc atgaagatga cccggacaag gtcgccttcc
     2581 acagaagaga cggtaagggg tatgcgctcg cccttttcat cgatgcggat cacaaggaac
     2641 tgccctgcaa gggcatgccg ggccacgtgg ggggcctcca cccatatctg gaatatccta
     2701 tcggccagct ggccggcctc gtacacccgg tacaatggtt tcactccgag aacgccaacg
     2761 agatggcttt cgaatagtta tgtgcgcggg agtgtcttta ccttacgcac cctgactgcg
     2821 actccccgcc atgaccggag catctcgtct gcgggcatgg cagcccttcc cgtggcaatc
     2881 gcgcagggcc cggtcacgag cacctcatcc ccttccctga tgtgcgggtc tgcatccacc
     2941 actccgggcg caagcacgtc cccggtaggg acgaactcat cgatgataac acggtaccct
     3001 tcagggatca tattccagcc ctcgaaggtg gggcggagaa ggccggtcgc ctcgtcgatt
     3061 gagaaagagg gggtcttccc cctgaacgcc ctgatccagg ggtagcggcc cttgatcacc
     3121 aggttcctcg tatcgatcgc gaggtcgaac tgccatgaag atgtcccccg gatggtgtca
     3181 tgatgaaccc tgttctcccc tgacagggaa gagtcaagaa atgcaagaga ttcgtgggac
     3241 gtgggcctgc ccgtgcaggt gatctccagt cccatgcctg cccgggccgc accgttctct
     3301 gccgccgcga gtgctccccc ttcgaggtgg gcgatgaccc ttttgaagcg gtgtttctcc
     3361 aggaattctc caatgatgcc gcccacgaac tccctctcct ctgcatccca gtagccggtg
     3421 accggcacat cgtagtgcgc cgcaggatag accagctcga gttccctcgg cacgagcccg
     3481 aggggcgagg tgacgatgag ctccagggcg cgcccggcga ttgcctgcct gaacctccgg
     3541 tggctctgtg aggaggagta gggcttcctc gccgagcagg ggaggaggac tgcaacgtca
     3601 gcgggaggcg gccggtaccg gcccacgacc cgttcagaaa acctggccac ctcgggcctc
     3661 tggagtactt cgccggtcgt cgcaaggagt gttcctgccc gcgcgatggg gactgccttc
     3721 tcaaagaacc cgcgagccct gtcgagatgc cggagaattg ccacctgcga ggcatccatc
     3781 ctgcaccggc tctccacaag gtcgcggagc ctctggcatg cgatatactc gcgtgcaagg
     3841 gccagttccc ttacgagggc aagccggttg tgctccttca ggtcgccgct tcggcacccg
     3901 gggcaggtgc agacgccgct cccgatccgg tccccgggga attcaccctc cgggatgcag
     3961 aacctgccct gcgcagagcg gaggtccacc gcggtgaaat cgaggatatc gaaacccgag
     4021 taacagagga tcgcggcatt cgaaggaagt gccgctccag gtgcgtacca ggcggtgtcc
     4081 ggagggaggg ctgatttcat cgctttcagc cactccacgt acctcctcgg attggcaaaa
     4141 gcggaatgcc agcacgggac catgacacag gtcccgtgag ccgggatatt ctctccagag
     4201 ggatggaccc aggcggggaa gtcccggtcc ctgggcaggt actgcgccac aaacgccgga
     4261 tctgcgccga gcgggacgtt ctccatcctg gagcccgaca gcccatcgaa caaactcccc
     4321 gtatcgagga cggttgggag atgataggtc ttcccctcag ctatgagggt tgccatcctg
     4381 gcaagcccgt ctcttttc
//
LOCUS       DAOQVJ010000063         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22210_length_4396_cov_4.019581, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000063 DAOQVJ010000000
VERSION     DAOQVJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22210_length_4396_cov_4.019581"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKL26_02190"
     CDS             <1..620
                     /locus_tag="PKL26_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNS82536.1"
                     /translation="NGSGMHTHGSLSKNGKNAFYDPKDERQLSDTAYYYIGGLLEHAK
                     AITRVANPTVNSYKRLVPGYEAPVYISWSAANRSALVRVPAARGNSTRAEFRSPDPMC
                     NPYLCFAAMLAAGLDGIRKKIEPPESTDVNIYHLSEKERRSRGIEMLPGSLIEANAEL
                     NNDDVIRNAMGTHIMEGLNSIAQLETDSFRLAVHPWELDRYLAMY"
     gene            876..1205
                     /locus_tag="PKL26_02195"
     CDS             876..1205
                     /locus_tag="PKL26_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNS82537.1"
                     /translation="MKCISAIVREEKVPFIQKALEKEGIFGMTLKGVMGRGEQRGIAL
                     QYRAGIFAVDLLPKVRIDVVVPGYLEEAAVKAITTQAFTGRPGDGRIFVIDVPQSIRI
                     RTSEVEA"
     gene            1769..2965
                     /locus_tag="PKL26_02200"
     CDS             1769..2965
                     /locus_tag="PKL26_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNS82538.1"
                     /translation="MALDTGTTAWLLASTALVMLMTPGVGFFYGGLVRRKNFISMVAL
                     SFIVFALVSIQWVVFGYSLAFGQDIGGFIGDLQFAGLQGIGMEAGDQGYPPLLFVAFQ
                     LVFAAIAMAIVTSGFAERVKLSAFLVFALLWTTLVYDPLAHWAWGGGWAMAFGALDFA
                     GGTVVHISSGFAALAVALVIGKRAGFGKYTMEPHNIPMTLLGAALLWFGWFGFNGGSA
                     LAADSIAASAFLVTNISAAAGGLAWLAASWVKGKPSSLGMVSGAIAGLVAITPAAGYV
                     TVMGSIAIGLVAGLLCYGCMLWRIRKGLDESLDAWSIHGMGGLWGAIATGVFAVAAVN
                     GYSGIIEGNVTQFLANAAGALAALVYAFVVTLVLAVAVDRVIGLRVSEEEEYVGLDIS
                     QHGERV"
     gene            2978..>3150
                     /locus_tag="PKL26_02205"
     CDS             2978..>3150
                     /locus_tag="PKL26_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNS82539.1"
                     /translation="MKLIKAIIRPERLEFVKKALEEKGTYGMTVQEVSGRGDQKGIRL
                     QYRGGTMVVDLIP"
     assembly_gap    3151..3250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3251..3564)
                     /locus_tag="PKL26_02210"
     CDS             complement(<3251..3564)
                     /locus_tag="PKL26_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS82540.1"
                     /translation="MNAGLDHPGEGSSYEQWRTASEMEKEGRSAVAVLIHNLHSDDKW
                     DRIAAADALGNIGDRNTTGPLAKLMLDRDHDVRFATAVALGKLGGATAMQALEQASRD
                     SN"
     gene            3751..>4396
                     /locus_tag="PKL26_02215"
     CDS             3751..>4396
                     /locus_tag="PKL26_02215"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS82541.1"
                     /translation="MWPLSGTDTLDTRSCLRGILVLALILAGISAHVQGASSIDFTAN
                     VTTGNVPLNVLFTDTSSLPVIDDSRRWDFGDGTVTFDAVGTISHTYQQEGTYTVRLDR
                     TDSEGYHSLIKYDYIIATTPTPTPTPTITTVPTTSPVTTATTVIPPTTPITTVPTTAP
                     TTQPTMQIPSEFFGEATSSGLPVPVGGRIVARIGSRNAGEIQVGSPGSYGGPGPF"
BASE COUNT          956 a         1133 c         1238 g          969 t
ORIGIN      
        1 tcaatgggag cgggatgcat acccatggat cgctctcgaa gaacgggaag aatgcattct
       61 atgatcccaa agacgagagg cagctctctg acaccgcata ttattacatc ggcggccttc
      121 ttgagcacgc aaaggccatt acgagggtcg ccaaccccac cgtcaactcg tacaaacgtc
      181 tcgtcccggg ttacgaagct cctgtgtata tcagctggag cgcagcgaac agatcagcgc
      241 ttgtaagggt tcctgctgca cgcggaaaca gtaccagggc tgaattccgt agtcctgacc
      301 cgatgtgcaa cccgtatctc tgttttgccg ccatgcttgc agcaggcctt gatggtatca
      361 ggaagaagat cgagcctccg gagagcactg atgtgaacat ataccatctc tctgaaaagg
      421 agaggagatc acgcggaatc gagatgctcc cgggcagcct gatcgaggcg aacgcggaac
      481 tcaacaacga cgatgtgatc agaaacgcga tgggcacaca tatcatggaa ggcctcaatt
      541 ccattgctca actggagact gattctttca ggctcgcggt gcatccctgg gagcttgacc
      601 gctacctggc gatgtattaa tttttttctt ccaaagaaaa caaaaactat ttatgtatac
      661 atcaataatt tccttccatc agattatagg aacagaccac gcatggatgc tgacagtgaa
      721 ggagatgaaa ggaatccagt cctgttggtg agtcctctct caaaggagat ggcggggacc
      781 tgctggcttc ccttcttttc tggaaacgaa ggaaaagcat cacttcatga cacggagcct
      841 ggatgcattc ccacacaaac cggggatgat gcgagttgaa atgcatttcg gcaattgtcc
      901 gcgaagagaa ggtcccattc attcagaaag ccctggagaa agaggggatt tttggaatga
      961 cactaaaagg ggtgatgggg aggggagagc agcggggaat tgcgctccag taccgcgcgg
     1021 ggatcttcgc agtcgatctt ttgccgaagg tgaggatcga cgttgtcgtt cctggatatc
     1081 tcgaagaggc ggcagtgaag gctattacta ctcaggcctt cactggcaga cctggtgatg
     1141 ggaggatatt tgtgattgat gtaccgcaat caatccgcat acggacaagt gaagtggagg
     1201 cctgatatgt agccatacga ttggacctcc cgaaaaaaat ctcctggccg ggattatacc
     1261 ggtcaatgtg ctctagaatg ccggggaatt tccccggcgt tccgcgtcta taaaaaggag
     1321 atttaccttt tgttagaaga tcaattttct atctccctct cacatcagat tcttggagtt
     1381 tcagcatctt accatttatt ggactaaaaa gaaaactata aataccaagg aaccacactt
     1441 ctacccatca aatgagttta ctcctaccca gtcaccaggg cagaagtccg cgtcccttca
     1501 ctctcataga gaagggatgg ggaatacctg taaaaacggg tgtacaaccg gatgaccgga
     1561 atcacgggag ccatttgata tacaggatca cggaaggatg ctgccggaca aggagctaaa
     1621 taatccctcc gcacaggaga tgggctctcc catgaattga ggggtggaaa ggtgcctggt
     1681 cctgaagtac ccggccaggg tccggaacag tatcgttgca ggctgactat ctggaacaga
     1741 atcagaagaa ggattcagga gattgacaat ggctttagat acagggacca ccgcatggct
     1801 gcttgcttct acggccctgg tgatgttgat gacaccgggc gtgggcttct tctatggggg
     1861 tctggtcagg agaaagaact tcatctcgat ggtggccctc tcgttcatcg tctttgcgct
     1921 ggtcagcatc cagtgggtgg tgttcggtta ttcactggca tttgggcagg atattggtgg
     1981 gttcatcggg gatctccagt ttgcaggcct gcagggtatc gggatggagg ccggggacca
     2041 ggggtatccg ccactgctct ttgttgcctt ccagctggtc ttcgcggcta tcgccatggc
     2101 tatcgtgaca tcgggctttg ccgagagggt gaaactctct gcgttcctgg tctttgccct
     2161 cctgtggaca acccttgtct acgaccccct agcgcactgg gcatggggtg gaggctgggc
     2221 catggcattc ggcgcacttg acttcgccgg cggcactgtc gtccacataa gctcgggctt
     2281 tgcggccctg gcagtggcac tggtcattgg aaaaagagca ggtttcggga agtacaccat
     2341 ggagccgcac aacatcccca tgaccctcct cggtgctgcg ctcctgtggt ttgggtggtt
     2401 cggattcaat ggggggagtg cgcttgcggc tgacagcatc gcagcgagtg cattcctggt
     2461 cacaaacatc tccgctgcgg ctggaggcct tgcctggctc gctgcaagct gggtaaaggg
     2521 aaaaccaagt tcgcttggca tggtgagcgg ggcaattgca gggctcgtcg caatcacccc
     2581 agctgcagga tacgtgacgg tgatggggag tatcgcaatc gggcttgtcg ccgggctgct
     2641 ctgctatggc tgcatgctgt ggcgcatcag gaaaggcctt gacgagagcc tcgatgcctg
     2701 gtccatccac ggaatgggcg ggctgtgggg tgcgatcgcg actggtgtgt ttgcggtcgc
     2761 tgccgtgaac gggtactcgg ggatcattga agggaatgtc acccagtttc ttgccaacgc
     2821 agccggggcc cttgccgcgc tcgtgtacgc cttcgtggtc acacttgtcc tggctgtggc
     2881 agtggacagg gtgataggtc ttcgggtcag cgaagaagag gagtatgtcg gccttgatat
     2941 ttcgcagcac ggcgagcggg tttaggaagt ggtggcgatg aagctgataa aggccattat
     3001 ccggcctgaa aggctggagt tcgtgaaaaa ggccctcgaa gagaagggta cgtacgggat
     3061 gactgtgcag gaagtgtccg ggagggggga tcagaaaggg atccggctcc agtatcgcgg
     3121 ggggaccatg gtggtggatc tcatcccgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn tggtttgaat ccctgctcgc ctgttccagt gcctgcatgg cggtggcgcc
     3301 gcccagtttt cccagggcga ctgcggtagc aaacctgaca tcgtggtctc tgtccagcat
     3361 gagctttgcg aggggacctg ttgtgttcct atccccaata tttcccagtg catctgccgc
     3421 agcgatacga tcccacttgt catcgctatg caggttgtgt atcaacactg ccacggcaga
     3481 acgcccttct ttttccatct ccgaggcggt cctccattgc tcgtatgaag aaccctctcc
     3541 cgggtggtcg agcccggcat tcatgtgaca tcccccttga ccctggcggg tcaaccgcag
     3601 ataggtgtga ggatacttca agctatgcat tgcacctgtg caccactggg ggcgttctcg
     3661 ttcccggcgc cggtctcctt gtgcaatgag aatatatatc tgcctgtgtg gagacacatt
     3721 gcgtgaatga attcccgcgg tgagtgggtg gtgtggccgc tgtccgggac tgacacgctg
     3781 gacacgaggt catgcctgcg aggcatcctt gtccttgccc ttatccttgc aggcatatcg
     3841 gcccatgtgc agggcgcatc cagcatcgat tttactgcaa atgtcactac cggaaatgtt
     3901 cccctcaacg tcctcttcac cgacacgagc agtcttccgg taatcgatga ctcaagaaga
     3961 tgggactttg gagacgggac cgtcacgttc gatgcggtcg gcaccatatc ccacacctac
     4021 caacaggaag gaacctacac ggtccgcctg gaccgaacag acagcgaagg ataccattcc
     4081 ctcataaaat atgactacat catcgctacc acgcctacgc caacgcccac ccctaccatc
     4141 accaccgtcc ccaccacatc acccgtgaca actgcgacga cagttattcc tcccaccact
     4201 cccatcacca cagtgcccac tacagccccg actacacagc cgacgatgca gatcccctcg
     4261 gaatttttcg gcgaggcgac ctcgtccgga ctccctgtgc cggttggggg acgtattgtg
     4321 gcaaggattg gatcaaggaa cgccggggag atccaggtgg gcagtcccgg gtcatacggg
     4381 ggtcccgggc ccttta
//
LOCUS       DAOQVJ010000064         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22614_length_4341_cov_3.591694, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000064 DAOQVJ010000000
VERSION     DAOQVJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22614_length_4341_cov_3.591694"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKL26_02220"
     CDS             <1..240
                     /locus_tag="PKL26_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNS82542.1"
                     /translation="IIRHLTIAGNVYDSLPTIGFEMIENERGISGLMYHFSKELCLMA
                     KDAAIVAYAHTYTLAHIVTNDRDFQRVKWLTCWGP"
     gene            641..958
                     /locus_tag="PKL26_02225"
     CDS             641..958
                     /locus_tag="PKL26_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS82543.1"
                     /translation="MTVEQSLVRAAPPAAPFYVSTDGVASKFRFYTFEPGWIGDWHPA
                     PTRQFLALMTGAVEMETTDGTVRRFLPGGLVLLEDTSGRGHLTRNVGDGYATYFVIPV
                     PVV"
     gene            complement(1235..1924)
                     /locus_tag="PKL26_02230"
     CDS             complement(1235..1924)
                     /locus_tag="PKL26_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNS82544.1"
                     /translation="MTHEKTATALLKIYSTLLDQYGNRGWWPADTPLETIIGAILAQN
                     VSWSNARKAIENLKAAGLLDVQVLRDADPTEISRHIISSRYYNQKARRISAFMQFLYA
                     EYGGDVDQMSGDDLPRLRMKLHSLKGFGEETVDSILLYACNVPVFVVDAYTKRIFSRY
                     GFLEKNATYQEVQHFFTAHLPKEVPLYNDYHAQIVNLGHSICRTHPDCHLCPIRSIPP
                     GIACEYEKNME"
     gene            complement(2382..2615)
                     /locus_tag="PKL26_02235"
     CDS             complement(2382..2615)
                     /locus_tag="PKL26_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNS82545.1"
                     /translation="MKTLIEVHKEGKYFVAVDLLTNVADQASSEEEAVQNLKKGLEEH
                     YQLLIELTPSNHKLTYLDIEVDTLIKNSSAVSS"
     gene            2941..3687
                     /locus_tag="PKL26_02240"
     CDS             2941..3687
                     /locus_tag="PKL26_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS82546.1"
                     /translation="MDIPRIFTISESAHRIHNPFTPENLATLGAALRLEPGARILDLG
                     SGSGEMLCTWARDYGITGVGIDMSTLFTKQAKLRAEELGVADRVRFIHGDAAGYVADE
                     KVDVAACLGATWIGGGVAGTIELLEKSLRPGGIILIGEPYWLKVPPTEDVARGCEANA
                     LSDFLLLPELLASFGHLNYDVVEMVLADREGWDRYEAAKWLTMRRWLDANPDDEFAKE
                     VRATLTLGPTRYATYTREYVGWGVFALMAR"
BASE COUNT         1042 a         1170 c         1083 g         1046 t
ORIGIN      
        1 ataatcaggc atttgactat cgcagggaat gtatacgata gtctgccaac gataggattt
       61 gagatgatcg agaatgaacg gggaatatca ggcctcatgt atcatttctc aaaagaactg
      121 tgcctcatgg caaaggatgc ggcaattgta gcgtatgcac atacctacac tctcgctcat
      181 atcgtcacga acgatcgtga ttttcagcgg gtcaaatggc tcacatgctg gggtccttga
      241 aaagagaaga gtatccctgc attctattcc actctcctcc tctcacctgt cgataccatc
      301 gacatgtccc aaacatgtgt cgaagaaatt gaaaaatttc gacacgttgg tcggccctca
      361 tcaaggagcc tgatgaggga ttgattgaac tacatccgtt ccttcccaat tttttcggat
      421 atcagaatgc cgatcctctc taattccttc agataccgcg cttcagtcta cacaaccttt
      481 atccatcatc attctgtttg tactttcacc ttgaggcatg ctctgcccga atattttaag
      541 gaggaactaa aacatggcac acacatcccc accccagctt aacaaggtaa cgtatcacag
      601 aatatcttcg gatacacagg ggggctcccg ctttgatacc gtgacggtcg aacagagtct
      661 tgtgcgtgca gcaccacccg ctgccccgtt ctatgtttcc acggatggag tcgcatcgaa
      721 atttcgcttc tatacgtttg agcccggttg gatcggtgac tggcaccctg cccccacccg
      781 gcagttcctg gccctgatga ccggggcggt ggagatggag acgaccgatg ggacggtgag
      841 gcggtttttg ccaggcggcc tcgtcctcct ggaggatacg tccggcaggg gacacctgac
      901 gaggaacgtc ggtgacgggt acgccaccta tttcgtgatt cctgtccctg tagtgtgaac
      961 cggggagatc atctcacgcg ttgttggctg gatgccggga ttccctgacg gtgggaagaa
     1021 ttccacaatc aagtacacgt ctttaaagaa ccatcccttc tatggtagtt atgtaccaaa
     1081 tgtccactct tggaggacat cacatggtga gaaaccattc ttacaaaacg aagggcgaag
     1141 gggtgttcca gtcaaccagg agggttggga tatctacgat acttctcacg taagaaaagg
     1201 cagaaagggt ggatgcatgg accgggaatt ctttttactc catgtttttc tcatattcac
     1261 aggcgatacc cgggggtatt gagcggatcg gacacaggtg acaatccgga tgagtcctgc
     1321 agatgctgtg tcccaggttc acgatctggg cgtggtaatc gttatacaaa gggacctctt
     1381 ttggcaaatg cgcggtaaaa aaatgctgga cttcctggta cgtggcgttt ttttccagga
     1441 acccgtaccg ggaaaagatc cttttcgtgt aggcgtcgac cacaaatacc ggaacgttgc
     1501 aggcatacag gagaattgaa tcgacggtct cttccccgaa ccctttgagt gaatgcaact
     1561 tcatcctcaa tcggggaagg tcatccccgg acatttggtc gacgtccccg ccatactcgg
     1621 catacaaaaa ctgcatgaat gcagaaatcc tccgggcttt ctggttgtaa tacctcgaag
     1681 aaatgatgtg ccgggatatc tctgtgggat cagcgtccct caatacctga acgtccagca
     1741 gtcccgcggc cttgagattc tctattgcct ttcttgcatt gctccacgac acgttctgcg
     1801 ccaatatcgc cccaatgatg gtctcaaggg gagtatctgc aggccaccat ccccggtttc
     1861 catactggtc caacaaggtg gaatagatct tcagcaatgc tgttgccgtt ttttcgtgtg
     1921 tcatgtcatg cccgcaggtg aatgcatgtc gggaaacagc cctggatcac tggggtgccg
     1981 ggttcctgga gaatcctccc agcagtgggg ttcccctatg ccactgaacc ggccaccgtg
     2041 gaaaccccat gagccaacgt aacgccagag atcgaagatt ctcgtcttct cctctcaggt
     2101 attccccaaa atccctaaaa aggtctttcc ttttcaatcc acacattgga gcttgactgt
     2161 ggagtgctct cttcttgtgg aaaaactatt ccttcacgtg ccggagaaat tcttccctgt
     2221 caatgtttgc ctgcctgagg atcgcgagca tggttccggt ggccaacgcc ggatggttgg
     2281 gaaccgtgac gagccgccgt gtttctggat gcaggagatg aatatggctg ccggtctgtc
     2341 atacagatgt atagccaaaa tctgcgagga tcctgataat ttcacgagct gacagcagag
     2401 gaatttttga taagagtatc cacttcaatg tcgagatacg tcaatttatg attgctggga
     2461 gtaagctcga ttaaaagctg gtaatgctcc tcaagacctt tcttgagatt ttgcaccgct
     2521 tcctcttcag atgatgcctg gtcggcaaca ttcgtaagga gatccacagc tacgaaatat
     2581 tttccttcct tgtggacttc aatgagtgtc ttcatgttca gatacaatct ttaggtagtt
     2641 ttatgagaat aagagtatgt tggtcacagt caggtacgtg tcccagccgg cgatcgccag
     2701 taccggggtg cccctccgct cccgccccat gccgatgtga aaacccggag agcaggtaaa
     2761 agtctgggcc gggaaaccgc gcccattatc cccggattca caaccccccc attcaccata
     2821 atccggctca tccgactcga aaccttaagg ctatggtgtt aacagttaca acctttctct
     2881 attacagtct ctaaatgtat attccagtta taccacaatt gagtaaaagg agcgtttacc
     2941 ttggacatcc cacgtatctt cactatatca gaaagtgctc accgcatcca taacccgttc
     3001 acaccagaaa atctcgccac tctcggcgca gccttgcgcc tggaaccggg tgctcgtata
     3061 ctcgacctcg gcagcggttc gggcgagatg ctgtgcacct gggcacgcga ttacggaatc
     3121 accggcgttg gcatcgacat gagcacgttg ttcaccaagc aggcaaaact ccgtgccgaa
     3181 gaactcggcg tcgccgatcg agtcaggttc atccacggcg acgctgccgg ctacgtcgcc
     3241 gacgaaaaag ttgatgtggc agcctgtctc ggtgctacat ggatcggtgg gggagtcgcc
     3301 ggcaccatcg agcttctgga aaagagtctc cgccccggcg gaatcatcct catcggcgag
     3361 ccctactggc tgaaggtacc cccaacagaa gacgttgcca ggggatgcga agccaatgcg
     3421 ctctccgact ttctcctgct tccagaactc ctcgcatcgt tcggccacct caactacgac
     3481 gtggtggaaa tggtgctggc tgaccgggaa ggatgggacc ggtacgaagc ggccaagtgg
     3541 ctcaccatgc gccgatggct cgacgcgaat cccgacgacg agttcgcgaa agaggtgaga
     3601 gccacactga ctttgggacc aacacgctac gccacctata cgcgtgaata cgttggatgg
     3661 ggtgtgttcg cgctgatggc gcggtgatgg gaggcagcgg ggatattcgg cggattgtca
     3721 tcggatgggg gcggctgagc tacactcggg ggctcctcga agtgtgagtg tatgtttcat
     3781 cacggaacac tcatgcaaca accaggaaac gttgagacta tggggggata tctcacacgc
     3841 caatccagta cagcttcaag aaattgaaag atttctctcc tctctccaat cctctcccaa
     3901 aatcccaatc caaaaccctt cccccccaca atttccaatc gcgactaata tctttgtcat
     3961 cttttggttc gacactccat tacagtaaaa acccgataat tctcatgaaa ctgaccgcaa
     4021 cggtgcgaaa gggggtcaca tcgccgctcc agcgaagggc agagatcccc tgcatcgcgc
     4081 ggtccgtcat cgtgctggaa ttcctgatcg ggatgcagcc atcatggtct ccagaacagc
     4141 acccctggtc gggatatccc tgcagcaaaa tctcctcccg cccaccagtg tctttcagaa
     4201 aagcctcggt ggacagaata cgatgtcccg taaaattctc tttcagaaat cactaaaaaa
     4261 aggagtggga atctcaatac ccattgtaca gtgccattgc acgatcgtag aacttcttcg
     4321 cttccaggag attgcccatc t
//
LOCUS       DAOQVJ010000065         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22620_length_4340_cov_2.846674, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000065 DAOQVJ010000000
VERSION     DAOQVJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22620_length_4340_cov_2.846674"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKL26_02245"
     CDS             complement(<1..916)
                     /locus_tag="PKL26_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS82547.1"
                     /translation="MYPDDHPDIQFIDPDTRFDYHEATSMIERYSAFEDLLNANSAKG
                     LVMPGSSPRPISPRTTIPVTPKATVSQTASLFHDPEYPKQLVAVIPAYNEELTIGTVV
                     LRTRQHVDHVIVVDDGSTDRTASVATLAGAEVLQHPINLGKAQAMFKGFARAREYNPL
                     AIVMLDADGQHNPDEIPELLKPVLAGKADLVVGSRYLNNKNDIPAYRVLGQKTLDLAT
                     NAGTGTRSTDTQSGFRVLGRRAMLNLDFYSEGYNIESDMLSHFAQRGLTIGEVPIGVS
                     YAVTNGHKKNPISHGLDVLSHIIGLVGYR"
     gene            complement(1223..1513)
                     /locus_tag="PKL26_02250"
     CDS             complement(1223..1513)
                     /locus_tag="PKL26_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82548.1"
                     /translation="MIFEVIVRKSAHVYLSGLPEKSRRIVKSALYSLGENPFPGTGGD
                     KEKLVLREGLTIYRIHIGHRYTAFYAIDTEHGIVRVHEILTIEQAHKRYGRL"
     gene            complement(1510..>1847)
                     /locus_tag="PKL26_02255"
     CDS             complement(1510..>1847)
                     /locus_tag="PKL26_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82549.1"
                     /translation="MPYFSSPPGGWVAGAGRRVGKGEIPITGCSPIVKVLIIYNMTPI
                     HMPAIKRIPVSEEVWKKLGDMKGAGQTYDDLLEDIIERAKKSRLEEDVRVWESSPDSE
                     YLPLSEIKD"
     assembly_gap    1848..1857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2168..2401
                     /locus_tag="PKL26_02260"
     CDS             2168..2401
                     /locus_tag="PKL26_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNS82550.1"
                     /translation="MNIPEGSDVDLLVNFEHGRSLLDQVGLIQDLEDLRGCRVDVVTE
                     AGLHWYIRDRVRKEGKDSLDSCNPPSGHHGKGV"
     gene            2497..2901
                     /locus_tag="PKL26_02265"
     CDS             2497..2901
                     /locus_tag="PKL26_02265"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNS82551.1"
                     /translation="MEKNAILEKMCELLRIFPEVQVAYCYGSFLLREDFRDIDIALFL
                     DENLTAEQQESSAEQIGIALEEAISFSHVCDIRVLDGAPVWFQYEVISSGRLLYARSE
                     EERYEKETSALIAYQDMKYTYDLFDSVYLAQA"
     gene            2905..3318
                     /locus_tag="PKL26_02270"
     CDS             2905..3318
                     /locus_tag="PKL26_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNS82552.1"
                     /translation="MRILAQLQALDDALEAWERYRQIPYEEFVREKDVRYMVCHALLL
                     AIQSTIDIATAIAVMKTPKRPDTYRETFLVLGRSSIIPEEIARELSTLAGFRNLLVHE
                     YTALDNGRVYRILQGDWQTLVAFRDIIREFVRENR"
     gene            complement(3439..>4340)
                     /locus_tag="PKL26_02275"
     CDS             complement(3439..>4340)
                     /locus_tag="PKL26_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNS82553.1"
                     /translation="SPVAPLSFLPMWPLLALSGAVAQAAYSTGVKVLLRRVSPYFVAG
                     ASFLPASGVLFLFSYLFGFPVLLEGFLPAVAATVAINVVATILSYRALAVSDLSLCLP
                     MLAFTPVFLLLTSFLILGETPSCFGLLGILLIAAGSYLLAAKGPGLLAPLRTLSRDAG
                     VRMMLIVAFLYSISVNYDKVVVESSDPVFGSAVVLGLLGAVFLAGAGLSAWGAPLLRG
                     EVRRSHLLLLPAIGLILVVEAVSINLAYTLSIVPYVISIKRLSIFFAVLFGGYLLREG
                     SIRARAAGSLVMVAGTALIALWG"
BASE COUNT          973 a         1105 c         1335 g          917 t
ORIGIN      
        1 tccggtaccc gaccagcccg atgatgtgac tcagcacgtc caggccatgc gagatcgggt
       61 tcttcttgtg gccattggtg accgcgtagc tcaccccgat ggggacttcc ccgatcgtca
      121 aacctcgctg tgcgaagtgc gacagcatgt cggactcgat gttgtagcct tccgagtaga
      181 agtcgaggtt cagcatcgca cgccgcccga gcacgcggaa ccctgattgc gtatctgtgc
      241 tcctggtccc ggtgccggcg ttggtcgcga ggtcgagggt cttctggccg aggacgcggt
      301 aggcagggat gtcgttctta ttgttgaggt accgggagcc gacgacgagg tcggcctttc
      361 cggccagaac gggcttgagg agttcgggga tctcgtcggg gttgtgctgg ccgtcggcgt
      421 cgagcatgac gatggcgagg gggttgtatt ccctggctct tgcgaacccc ttgaacatgg
      481 cctgggcttt cccgaggttg atggggtgct gcaatacctc tgcgccggcg agggtggcga
      541 cgctcgcggt cctgtctgtg gagccgtcgt cgacgacgat gacgtggtcg acgtgctggc
      601 gggtgcggag gacgacggtg ccgatcgtga gctcttcgtt gtaggcgggg atgacggcga
      661 cgagctgctt cgggtactcg gggtcgtgga agagggaggc ggtctgcgat accgttgcct
      721 ttggtgtgac agggattgtg gtgcgaggag agatgggccg gggagaggat cccggcatga
      781 ccaggccctt cgccgagttc gcgttgagca ggtcctcgaa ggccgaatag cgttcgatca
      841 tcgaggttgc ttcgtggtag tcgaaccggg tgtccgggtc gatgaactgt atgtctgggt
      901 ggtcgtctgg gtacattggg atgaccttgt tgccggattt ccggatcggt ttatatattg
      961 tactccccct tccgatccgg acccggattt taaatattct agtggaatct tatcattttc
     1021 ataaaataaa aactttgtgg aacttttttg gaaaaaaggg ttttggatgg cggcggaggg
     1081 gaatgggggg agtgtgtgtg gatttctggg aggtggggat tgtggggggg tgtggctctt
     1141 atcccgccac ccacccgccc agccatcgtt tgacaaatgt ggaggcgatg gagggaatct
     1201 tttctcgtgg gtataccgta atttacaacc gtccgtacct tttgtgagcc tgttcgattg
     1261 tgaggatttc atgaaccctt acaatcccat gttcagtatc tatcgcataa aaagccgtat
     1321 agcggtgtcc gatatggatc cggtatatgg tcaaaccttc ccgcaggacc aatttttctt
     1381 tgtcaccacc ggtgccgggg aatgggttct caccaagact atacaaagcg cttttaacga
     1441 ttcggcggga tttttcaggt aaaccggaga gatacacgtg tgctgacttc cggacaatta
     1501 cctcgaagat caatctttga tctccgagag cggcaggtat tctgaatctg ggctactctc
     1561 ccagactctc acgtcttcct caaggcggga tttttttgcc ctttctataa tatcttcaag
     1621 aagatcgtca taggtctggc ccgctccctt catatcgcca agctttttcc agacctcttc
     1681 ggacacggga atacgcttta ttgcgggcat atgtataggt gtcatattgt aaattattaa
     1741 aacctttaca atgggtgaac atcctgtaat gggaatttca ccctttccca cccgccgtcc
     1801 ggcccccgcc acccacccgc caggaggaga ggaaaaatac ggcatcgnnn nnnnnnngcg
     1861 cgacggaatc ccggcccgtc tgacaagggg gtacggccga agagccagcc tcttgacaag
     1921 atagggtagc ggggtatact ataaaggcat gagccatatt gcccgggaac gcaataaaat
     1981 cccgatgcgt gagccagatt gccctccaga atcattatat ggatttttac acaatgtctg
     2041 gtgagaaggt gagataagat accagatctc gcgacatctg ctcaaggaaa aacgcggaag
     2101 atattctctc gcattgcgtc acaatacggt gcaacaaatg tccgcgtgtt cggatcggtg
     2161 caacgggatg aatattccgg aagggagtga tgtggatctt ctcgtcaact ttgagcacgg
     2221 gaggagtctc cttgaccagg tgggattgat acaggatctc gaagatttgc gaggatgccg
     2281 ggtggatgta gtcacggagg ctggtttgca ctggtatata agggacaggg tccgaaaaga
     2341 ggggaaagat tcgctggatt cctgtaatcc gccctccggc caccatggga agggagtata
     2401 gcattcagga ggggagatcc tcggtactgc caagccccac ggcaaatact atttatattc
     2461 tcttgcgaat acctcttgac aggaggggag aggaaggtgg agaaaaacgc aattctcgag
     2521 aaaatgtgcg aactattgcg catatttcct gaagtgcagg tggcatactg ttacggatca
     2581 tttcttcttc gcgaggattt ccgggatatc gatatcgctc tcttccttga cgaaaatctc
     2641 accgctgaac agcaggaatc ttccgctgaa cagataggaa tcgcgctgga agaggccatc
     2701 tccttttccc atgtgtgtga tatccgggta ctcgacggtg cgcccgtgtg gttccagtac
     2761 gaagtaatct cgtcagggcg actgctctat gcccggtccg aagaggaacg gtatgagaaa
     2821 gagacttcgg cgctcatcgc ataccaggac atgaaataca cctacgacct cttcgactcc
     2881 gtgtacctgg ctcaggcctg aaccatgcga atccttgcac aactccaggc actcgacgat
     2941 gccctcgagg catgggaacg ttacaggcag ataccctacg aggaatttgt ccgggagaag
     3001 gatgtgcgtt acatggtatg ccatgccctg cttctggcaa tccagtcgac gatcgacatc
     3061 gcgactgcga tcgctgtgat gaagacccca aagagaccgg atacctacag ggaaacattt
     3121 cttgtgctcg gacgatcatc gatcataccc gaagagatcg cgagggaact ctccacgctt
     3181 gcgggattcc gaaacctcct ggtccacgag tatacggcgc tcgataatgg gcgcgtgtat
     3241 cggattttac agggagactg gcagacgctg gtggcgtttc gggatattat cagggaattt
     3301 gtaagggaaa accggtaatt ctgcaggatt tttcgtttcc acatgcgagc cggacagcaa
     3361 cacgtttcaa tccatgcggc agatggtgcg cccagcgatc gaatgtgaat cgattggccg
     3421 gggaaaatgc accccccctc acccccacag cgcgatcagg gccgtccccg cgaccatgac
     3481 gaggcttcct gcggccctcg cccggatgct cccttccctg aggagatatc ccccgaagag
     3541 gaccgcgaag aagatcgaga gcctcttaat ggaaatcacg taagggacga tcgagagagt
     3601 ataggcgagg ttgatcgaga ccgcctcgac tacgaggatc aacccgatgg ccgggaggag
     3661 aagcaggtgc gatcgccgga cctccccgcg gagaagcgga gccccccacg cagaaagccc
     3721 cgccccggca agaaacacgg cgccgaggag gccgagcacc actgcggacc cgaagaccgg
     3781 gtcggaggac tccaccacca ccttgtcgta gttgaccgag atgctgtaga ggaacgcgac
     3841 aatgagcatc atccgcaccc ctgcgtcccg ggagagggtg cggagcgggg cgagcagccc
     3901 cggccctttc gccgcgagga ggtacgaccc ggcggcgata aggagtatcc caaggagacc
     3961 gaagcacgag ggagtctcgc cgaggatgag aaaggaggtg aggagcagga agacgggagt
     4021 gaacgcgagc atcgggagac agagggagag gtccgacacc gcgagcgccc ggtaggagag
     4081 gattgtcgcc acgacattga ttgccactgt tgctgccacc gcggggagga agccctccaa
     4141 caggaccggg aatccgaaaa gatacgagaa gaggaagagc acccccgatg ccgggaggaa
     4201 cgaagcgcct gccacgaagt atggcgagac ccgccggagc agcaccttca cgccggtgct
     4261 gtaggctgcc tgggcgacgg cccccgagag ggcgaggagc ggccacatgg gtaagaaaga
     4321 gagtggagcg acaggagatg
//
LOCUS       DAOQVJ010000066         4339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22625_length_4339_cov_4.123016, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000066 DAOQVJ010000000
VERSION     DAOQVJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22625_length_4339_cov_4.123016"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="cofC"
                     /locus_tag="PKL26_02280"
     CDS             complement(<1..582)
                     /gene="cofC"
                     /locus_tag="PKL26_02280"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNS82554.1"
                     /translation="MPVVALIPFKPVNPKTRLSCVLEPGERESFARCMLADVIEAVSG
                     AGCEPRIISTEPYACRGATVEVIPGGLNETLNALLAQAGDPVLIIMADIPLATPGAVS
                     RLISTGRDMAIAPGRGGGTNAIYLSEGSSYRVDYYGASFLKHARIARERGLSCEVIDS
                     FRLHTDVDEKEDLVELLIHGRGRSREYLESLGFT"
     gene            704..1702
                     /gene="cofG"
                     /locus_tag="PKL26_02285"
     CDS             704..1702
                     /gene="cofG"
                     /locus_tag="PKL26_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HNS82555.1"
                     /translation="MPQRTITYSRNVFLPLTTVCHNRCGYCSFRTPVHEDCVMSPGQV
                     EDILQRGAALGCTEALFTFGERPGLEPGFSRILGKMGYRDIIDYCYDLCEKAIGIGIL
                     PHTNAGILTMEEMDRLSEVNASMGLMLETTARVDAHRNSPGKVPAVRIGMIEDAGKLR
                     IPFTTGILLGIGEGMDDREESLMVIRDLHKRFGHIQEVIIQNFCPKDKTPMAGCSPVE
                     PEEICLTIRLARDILPGEIAIQIPPNLADAASLIPCGVDDLGGVSPLTIDYVNPEHPW
                     PEIRELKRLAGDARLKERLCIYQRYIDKGWCPPRLAPLISRLNQMIQHRSDRSAEG"
     gene            1689..>2224
                     /locus_tag="PKL26_02290"
     CDS             1689..>2224
                     /locus_tag="PKL26_02290"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNS82556.1"
                     /translation="MQKGDLLYAGKAKSVFLTDREGILLVEFRDDITAFDGGKKDILD
                     KKGCFNAKVSAYIFRLLTENGIPTHFIDMVDNRRMLVRSLSMIPLEVIVRNVAAGSMS
                     RTYPIAEGTPLSPPVIVIDYKDDSRHDPMLNDDLILALKLLSAQELARVKDLALAVNS
                     VLVRFFESLGITLVDFKI"
     assembly_gap    2225..2324
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2325..2528
                     /locus_tag="PKL26_02295"
     CDS             <2325..2528
                     /locus_tag="PKL26_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNS82557.1"
                     /translation="LNPDTKKLDIVVRGLARNQRKFGERYCPCRLRSGDPEKDRDIIC
                     PCVFHRDEVERDGSCHCNLYFRK"
     gene            complement(2613..>4339)
                     /locus_tag="PKL26_02300"
     CDS             complement(2613..>4339)
                     /locus_tag="PKL26_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNS82558.1"
                     /translation="IVNLALTGGLFGLLPLPLARYDDMVTRFRRYWQIADVLFKYGFE
                     IFVQRLFPGVHRFRLRKSSPVDSAASEYQRMRMALEELGPTYVKFGQILSTRQDMLPP
                     GLIEELKRLQDKTNPLPFETIMGVLENACPGYREYFSEIEEIPVASASLSQVHRARLH
                     DGTPVALKVQRPGIEEIIETDIVILESAARRGERSFPEWKVYNPRGIVRDFAVQIRKE
                     LDFLRDGKNADLLRVHMRDLEGVKIPRIYWKYSSRHLLVMEYIEGVRVDNVEAIRAYG
                     HSPKRIAEAGFFAYMKQIFEDGFFHGDPHPGNLIVTKEGELVFLDFGIIGIIRPERRF
                     WFVQLLNSMVLLDAGMLMKSMEGLSVKIPEESRELLRDEIYTAMLEAEGISIGQFNFT
                     AMTNAFTTILREYRIQVPGNLMLMLKVIIMVLDVGVTLNPEFRFKDQAESYMARFRKS
                     ESIVDQLRSRAGGSMLEAMDGFLDMPRNINQMLKQLSTGIIRIDVVDTDIRRLQMSLD
                     RTSNKVLIGLVVSGMVVGSSFILRETQLPVPGVVFYIAILAYVIALCIGFYAIYKVLS
                     TGVDEKER"
BASE COUNT          918 a         1288 c         1144 g          889 t
ORIGIN      
        1 ggtaaaaccg aggctctcca ggtactccct gctcctccct cgcccatgga taaggagctc
       61 gaccaggtct tccttctcgt cgacatccgt gtgcagccgg aacgagtcga taacctcgca
      121 tgagagcccg cgctcccggg caatccgggc atgcttcagg aaactggcac cataatagtc
      181 aacacggtag gaagatcctt ccgagaggta tattgcattg gttcccccgc ccctgcccgg
      241 cgcgatggcc atgtcgcgcc cggtggagat gaggcggctc accgcgcccg gagtcgcgag
      301 tgggatatct gccatgatga taaggacggg gtcgccggcc tgtgcgagga gcgcgttcag
      361 ggtctcgttc agtccgccgg gaatgacctc gacagttgcc cccctgcatg cgtagggctc
      421 cgtggatatg atccgcggct cgcatcccgc accgctgacc gcctcgatca cgtccgcgag
      481 catacacctc gcaaaggact cccgctcccc cggctccagc acgcacgaga ggcgtgtctt
      541 tgggttcacc ggcttgaatg gtataagcgc aacgaccggc atgcctgctt cttttccgtt
      601 tgtgtccatg accacaaaat acctcttgat ttggaatgcg ccgcacccct gccacatatg
      661 gtccattcat tagggatgaa accgattgaa catgggacga gagatgccac aaaggaccat
      721 cacttactcg cgcaacgtct ttttacctct caccaccgtt tgccacaacc ggtgcggcta
      781 ctgctccttc cgcacccccg tacacgagga ttgcgtgatg tccccggggc aggtggaaga
      841 catcctgcag agaggtgccg cacttggatg cacggaggcc ctcttcacct ttggggagcg
      901 gccaggcctc gaaccggggt tttccaggat actcggaaag atggggtacc gggatatcat
      961 cgactattgt tacgatctct gtgagaaggc catcggcatc gggatactcc cccataccaa
     1021 tgccggaatc ctcactatgg aggagatgga tcggctctcg gaagtgaatg caagcatggg
     1081 cctgatgctc gagacgacag cccgggtgga cgcccaccgg aattcgcccg gcaaggtccc
     1141 cgccgtccgt atcgggatga ttgaggacgc tgggaagctg cggatcccct tcaccacggg
     1201 catcctcctg ggaattggag aagggatgga tgatcgggaa gagtcgctca tggtcatccg
     1261 cgacctccat aaaaggttcg gccacatcca ggaggtgatc atccagaact tctgcccgaa
     1321 ggacaaaacc cccatggcag gttgctcccc cgtcgagccc gaggagatct gcctgaccat
     1381 ccggctcgca agggatatcc tccctggcga gatcgccatc cagatcccgc cgaacctcgc
     1441 ggatgctgca tccctcatcc cctgcggtgt tgacgaccta ggcggcgtct ccccgctcac
     1501 catcgattac gtcaatcccg aacatccctg gcccgagatc agggaactca agcgccttgc
     1561 cggcgatgcc cgtctcaagg agcggctgtg tatctaccag cgctacatcg acaagggatg
     1621 gtgcccgcct cgtctcgcac cacttataag cagactgaac caaatgatac agcataggag
     1681 cgatcgcagt gcagaagggt gacctgctct acgcgggcaa ggcaaaatcg gtcttcctga
     1741 ccgacaggga agggatctta ctcgtggagt tccgcgatga catcaccgca ttcgatggag
     1801 gaaagaagga tatcctcgac aagaaaggct gtttcaatgc aaaggtctcc gcctatatct
     1861 tccgcctgct gacggaaaac gggatcccga cccatttcat cgacatggtg gacaaccgca
     1921 ggatgcttgt ccggtccctc tcgatgatac cactcgaggt gattgtccgg aatgttgctg
     1981 ccggttccat gagccgcacg tacccgatcg cagaggggac gcccctctcc ccgcctgtca
     2041 tcgtgatcga ttacaaggac gattcgcgcc atgaccccat gctcaacgat gaccttatcc
     2101 ttgccctgaa gctcctcagt gcacaggaac ttgccagggt caaggatctc gcccttgcag
     2161 tcaattccgt gctggtgcgg ttctttgaat cgctcgggat aacccttgtg gatttcaaga
     2221 tcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcaac cctgacacga
     2341 aaaaactgga catcgtcgtc cggggacttg cccgaaacca gaggaaattc ggagagcgct
     2401 actgcccttg ccgcctccga tccggcgacc ctgaaaaaga ccgggacatc atctgcccct
     2461 gtgtcttcca ccgggacgag gtagagcggg acggcagctg ccattgcaac ctgtatttcc
     2521 ggaagtaagg cgggtaggga tcacgatcgt tcctgggatg ttgtagtccc ggcccttttt
     2581 ctctgtgaca tgtatttccg gatagaataa ttttagcgtt ccttttcgtc aacgcctgtc
     2641 gaaagaacct tgtagatcgc atagaagcct atgcagaggg caatgacata ggcgaggatc
     2701 gcgatgtaaa atacgacgcc cggcaccggc agttgagtct cccggaggat gaacgacgaa
     2761 ccgaccacca tgcccgagac aacgagtcct atcagcacct tgttgctcgt ccgatccagc
     2821 gacatctgca accgccggat atcggtatcg acgacatcaa tcctgatgat ccccgtcgag
     2881 agctgcttca acatctggtt gatattcctt ggcatgtcca ggaatccgtc catggcctcg
     2941 agcatggagc cccctgccct tgacctgagc tggtccacga tcgactcgct ctttctgaac
     3001 ctcgccatgt acgattcagc ctggtccttg aaccggaact ccgggttcag ggtcactccc
     3061 acatcaagga ccatgatgat caccttcagc atcagcatga ggttccccgg cacctggatg
     3121 cggtactcgc ggaggatggt ggtgaatgcg ttggtcatcg ccgtgaaatt gaattgccct
     3181 attgagatcc cctcggcctc gagcattgca gtataaatct cgtcgcggag tagctccctt
     3241 gactcctctg ggatcttgac cgagaggcct tccatcgact tcatcagcat ccccgcatcc
     3301 aaaaggacca ttgaattcag gagctggaca aaccagaaac ggcgttcagg ccggataatc
     3361 ccaatgatac caaagtcaag aaaaacaagc tcgccctcct tcgtgacgat gagattgccc
     3421 gggtggggat cgccgtggaa gaacccgtcc tcgaagatct gcttcatgta ggcaaaaaat
     3481 ccggcctctg caatcctttt cgggctgtgt ccgtacgccc ggatagcctc cacgttatcc
     3541 acccgcacac cctcgatgta ctccatcacc agcaggtgcc tgctgctgta tttccagtag
     3601 atcctcggga tcttcacgcc ttcaagatcg cgcatgtgga cacgcagaag gtcagcattt
     3661 ttcccatcgc gcaaaaaatc cagctctttc ctgatctgca ccgcaaaatc ccttacgatc
     3721 ccccgggggt tatacacctt ccactctgga aagctcctct ctcctcgcct ggcagcggac
     3781 tcgaggatca cgatatcggt ctcgatgatc tcttcaatcc cgggcctctg cactttcaga
     3841 gcgacagggg tgccatcgtg cagccgggcc ctgtgaacct ggctgaggga tgcggacgca
     3901 accggtatct cctcgatctc gctgaaatac tccctgtatc cggggcaggc attctcgagc
     3961 actcccatga tcgtctcaaa cgggagcggg ttggtcttgt cctgcaggcg cttgagttcc
     4021 tcaatcagcc cgggcggaag catatcctgc cgggtgctga gaatctggcc aaatttcacg
     4081 tatgtgggcc cgagttcctc gagggccatc ctcatccgct ggtattcgct ggcggcagaa
     4141 tcaaccgggc tggatttgcg gagtcggaac ctgtgaactc caggaaagag gcgctgaacg
     4201 aagatctcga acccgtactt gaagagtaca tcggcgatct gccagtagcg acggaatctg
     4261 gttaccatgt catcatacct ggccagggga agtgggagaa ggccaaaaag ccctccggtg
     4321 agtgccagat taacgatgg
//
LOCUS       DAOQVJ010000067         4338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22634_length_4338_cov_4.310530, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000067 DAOQVJ010000000
VERSION     DAOQVJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22634_length_4338_cov_4.310530"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /gene="metG"
                     /locus_tag="PKL26_02305"
     CDS             complement(<1..1932)
                     /gene="metG"
                     /locus_tag="PKL26_02305"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNS82559.1"
                     /translation="MSEKPILVTCGLPYTNGPCHIGHLRTYIPADCYVRYLRRRGEEV
                     VFVCGSDNHGTPIVVSAEEAKISPREMSERYHAHFDLTFRRMKVEFDHFGMTDDPTNH
                     SRTKQIVETLAKNGFIYSKVIKQSYCTKCRRFLPDRYVEGTCPHCGVQARGDECDQGC
                     GRHLEPGEIRDPVCMVCKTKAELRDQEHYFFKLSEFKEFLLQYLDGLAGTLNARNYAL
                     GWVKEDLHDWCITRTLDWGVKFPGRDDLVVYVWVDAPIGYISFTEEWAKKTGNDWKAL
                     WCGENRVTHFIGGDIIYHHCIFWPALLKGAGYGTPYSVVASGMVKVDDHKFSKSRGYV
                     VWTNDDYLDRGLPADYLRYYLLSYTNHTKDLNFSWKVFGERVNNELVNTLGNFIYRSL
                     YFAHKEFGGIPDVPAREEIFAQIRQSFSRVDNAMCDYDFKVAVDEMMALAAFGNNYIQ
                     SSAPWKLIKTDRRAAAQVVKDCLVLARALCLLSEPVLPEKAREIWAMLGNSSHMDGCI
                     LEDALSDLSPCALPAPRPLFPKLEDAFISEMDTLLQKRVEEAKKRSQREVPVSIEEFS
                     RLDIRTGKILSAEPIPKSTKLLKLQVDIGDERRQIVAGIAPFYDPADLIGVPVVVVAN
                     LAPAKIFGVESRGMLLAAGD"
     gene            1975..2769
                     /locus_tag="PKL26_02310"
     CDS             1975..2769
                     /locus_tag="PKL26_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82560.1"
                     /translation="MAKKLVFQTVSMGSEPPIPEAGELSEWVRTQRGRQADLTSFLLE
                     EGLLPQKEAEVDFPCSGGTFYHKRMRESISGIKDGYITHELGLMPEFVEDDAARVRSV
                     SGGSRVALPAPHLLDLTDRYYGDVDESSAALCQQYRRLLRAMRDAGVAGHVLHCADAV
                     PEELESLAGKKVFFFLEEPDEESIATLLEYQRVIAIRRDLLPVLLSHREEYEVTEVLI
                     MDPDAMDLKNLNHSFDPDQIRVGGYWKGNNLEYWRDLVDSATVSLQ"
     gene            complement(2758..3732)
                     /locus_tag="PKL26_02315"
     CDS             complement(2758..3732)
                     /locus_tag="PKL26_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNS82561.1"
                     /translation="MKFPWTAPPRKGRDEELQDQLQKRPRGVVHITHNDLDAVGCDAI
                     HRRVFDPVTTVFCSVGRFPHVMSEISRMSGEGDTLSISDLGYYQGAESVVDTLREKGW
                     RVEWRDHHRWRDEERARVEKRCNLLCVQVDTCATGICARDLAPDDPVALEVARVVCDY
                     DLWIHADPRSAVLGQVMQRKKNREYVRDCLMQGIFSDARIMAQYREIREEMERLMEKS
                     IRKAHIRGSRYRIAFAPLYGYPSETAAMMRSTLSTDMEVLISPSGRFSLRSVPPVSHI
                     IARNFGGGGHPNASGGNFPFSFWDGVLFRLFGRSPHIDRFVEIAESLE"
     gene            3832..3905
                     /locus_tag="PKL26_02320"
     tRNA            3832..3905
                     /locus_tag="PKL26_02320"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3866..3868,aa:Phe,seq:gaa)
     gene            complement(4079..>4338)
                     /locus_tag="PKL26_02325"
     CDS             complement(4079..>4338)
                     /locus_tag="PKL26_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS82562.1"
                     /translation="DYQFPLNILHDEGIYANVHIYPAIWEATYDSAEEAARHWVTLHY
                     PGLEDLSPVIDFFSRVLRRDISGRLVHSAIRRTAVIWWTKE"
BASE COUNT          903 a         1243 c         1194 g          998 t
ORIGIN      
        1 gtccccggcg gcaaggagca tcccgcggct ctcgaccccg aatatctttg ccggagcgag
       61 gtttgccacc acgactacag gtaccccaat gagatctgcc gggtcgtaaa atggcgcgat
      121 ccctgccaca atctgccttc tctcatcacc gatatccacc tggagcttga ggagtttggt
      181 ggattttggg atcggttctg cggagaggat cttcccggtg cggatgtcca ggcgggagaa
      241 ctcctctatg gagacgggca cctccctctg tgatctcttt tttgcctctt cgaccctctt
      301 ttgcagaagt gtatccatct cgctgatgaa cgcatcttcg agtttcggaa agagcggcct
      361 tggtgcaggg agtgcacagg gggaaagatc ggaaagggca tcctccagga tgcatccatc
      421 catgtgactg gaattcccaa gcatggccca tatctcgcgt gccttttccg gcagcacagg
      481 ctcggagagg aggcagagcg cccgtgccag gacaaggcag tccttgacca cctgtgccgc
      541 ggcccgccga tccgtcttga tcagcttcca gggtgcgctg ctctggatgt agttgttccc
      601 gaatgccgca agcgccatca tctcgtccac cgcgaccttg aagtcatagt cgcacattgc
      661 attgtcgacc cgggaaaaag actgacgtat ctgtgcgaat atctcttccc tcgcggggac
      721 atcaggaatc ccgccgaact ccttgtgcgc gaaataaagg cttcggtaga tgaagtttcc
      781 gagggtgttc accagttcgt tgttcacccg ctcgccaaag accttccagg agaagttgag
      841 gtcttttgtg tggttcgtat aggagaggag gtaataccgc aggtagtcgg cgggaagccc
      901 cctgtccagg taatcgtcgt ttgtccacac gacgtacccg cgggatttcg agaacttgtg
      961 gtcatccacc ttgaccatcc cgctggcaac gaccgagtat ggcgtcccat accctgcacc
     1021 cttgagaagg gccggccaga agatgcagtg gtggtagatg atatccccgc cgataaaatg
     1081 ggtcacgcgg ttctccccac accagagggc tttccagtcg tttccggtct ttttcgccca
     1141 ctcctcggtg aacgagatgt acccgatggg ggcatccacc cacacgtaga cgacgagatc
     1201 gtcccggccg ggaaacttca ccccccagtc gagcgtgcgg gtgatgcacc agtcgtgcag
     1261 gtcctctttc acccacccga gtgcatagtt tctggcattc agggttcccg caagaccgtc
     1321 aaggtactgg agcaggaact ccttgaactc gctcagtttg aagaagtagt gttcctgatc
     1381 gcgaagctcg gcctttgtct tgcataccat gcagaccggg tcccgaattt ctccgggctc
     1441 gaggtggcgg ccgcatccct ggtcgcattc atcgccccgg gcctgcacac cgcaatgtgg
     1501 gcaggtcccc tccacgtagc gatctggaag gaaacggcga cacttcgtgc aatagctctg
     1561 cttgatcact ttggagtaga tgaatccgtt ctttgcgaga gtttcgacga tctgcttcgt
     1621 gcgcgagtgg tttgtgggat cgtcggtcat cccgaaatgg tcgaattcaa ccttcatccg
     1681 gcggaaggtc agatcgaagt gggcatggta gcgttcagac atctcccttg gggagatctt
     1741 cgcctcttct gcactgacca cgataggcgt gccgtggttg tccgaaccgc agacgaatac
     1801 cacctcctcg cctctcctcc gcaggtacct gacgtagcag tcggcgggga tgtaggtccg
     1861 caggtgcccg atgtgacagg ggccattagt ataaggaagg ccgcaagtca ccagaatggg
     1921 cttctcactc atcactatgc ttttgatgat gatgctataa ataactgaag agacatggcg
     1981 aaaaagctcg tcttccagac agtttccatg ggttcagagc cgcccatccc ggaggccggc
     2041 gagctttccg aatgggtccg cacacagcgg ggaaggcagg ctgacctcac ctcgtttctc
     2101 ctcgaagaag ggctccttcc acagaaggag gcggaagtgg actttccctg ttccggcggg
     2161 actttctatc ataaaaggat gcgtgagtcg atttccggga ttaaagatgg atatattaca
     2221 catgaacttg gtttaatgcc ggaattcgtg gaggatgatg ccgcgagggt caggtctgtt
     2281 tcaggcggct ccagggtcgc cctccctgca ccccacctct tagatctgac tgaccgttat
     2341 tatggagatg tggacgagtc ctctgctgca ctctgtcaac agtatcgccg cctcttgagg
     2401 gcaatgcggg atgcaggggt agcaggccac gtgctccact gcgcagatgc agtccccgaa
     2461 gaactggagt ccctggcagg aaaaaaagtc ttcttcttcc tggaggaacc tgacgaagag
     2521 tctatcgcca cgcttctcga gtaccagcgg gttatcgcca ttaggaggga ccttctccca
     2581 gttctccttt cgcacaggga ggaatacgaa gttacggagg ttctcatcat ggaccccgac
     2641 gccatggatc ttaagaacct gaaccactcg tttgatccgg atcagatccg ggtgggtggg
     2701 tattggaagg gaaataacct ggaatactgg cgggatctcg ttgattccgc caccgtatca
     2761 ctccagtgat tctgcaatct ccacgaaccg gtcgatatgg ggggatctcc cgaacagccg
     2821 gaagaggacg ccatcccaga acgagaaggg gaagtttcct cccgaggcat tcgggtgacc
     2881 cccgcctcca aagttccttg caattatatg gcttacgggg gggacagagc ggagggagaa
     2941 ccgccccgat ggagagataa gcacttccat gtcggtcgaa agggtgctgc gcatcatggc
     3001 cgcagtctcg cttgggtacc catagagtgg tgcaaatgcg atgcggtacc tgcttccccg
     3061 gatatgcgcc tttcggatac tcttttccat gagcctctcc atctcctccc ttatctcccg
     3121 atactgtgcc atgatgcgtg catcggagaa gatcccctgc atcaggcagt ctctcacgta
     3181 ctccctgttc ttcttccgct gcatcacctg cccgagtact gcagaacgtg gatcggcatg
     3241 tatccacagg tcgtagtcac agaccactcg tgccacttcg agtgcaacag gatcatcagg
     3301 tgcaaggtcg cgggcgcaga tccccgtcgc gcaggtgtcc acctgcacac aaaggagatt
     3361 gcacctcttc tctacacggg cccgctcttc gtcccgccaa cggtgatggt cccgccactc
     3421 aactctccag cccttttccc ggagggtatc aaccacgctt tcagccccct ggtagtatcc
     3481 gagatctgaa atgctcagag tgtccccttc cccgctcatc ctggagattt cactcatcac
     3541 atgcggaaac cgccccactg agcagaagac cgtcgtaacg ggatcaaaga ctctccggtg
     3601 gattgcatcg catccgaccg catcaaggtc gttgtgggta atgtgcacta ccccgcgggg
     3661 gcgcttctgc agctggtcct ggagttcctc gtcgcggcct ttccgggggg gagcagtcca
     3721 gggaaatttc accggatcca ccgccacagc gcaggataaa atgggccttg cctcgtattc
     3781 atgccagaaa acacatcttt tattagtctg tactgacaat attgtttccc tgcctcagta
     3841 gctcagttcg ggagagcgcc agactgaaga tctggttgtc cccggttcaa atccgggctg
     3901 aggcactttg gagcttgggt tcaaatccct tcgatttttt tttaattttt actgcctatt
     3961 cttcatgatg ttgtaacgtt tgccggtttg ataggatctc attgaatcag tgattcggca
     4021 aatcaggaga cccaccacct attcctgcat atagtctctc cgtaaccctg ttcgcaactc
     4081 actctttcgt ccaccagatc accgcagtac gacggatcgc ggagtgcacc agcctgccgg
     4141 agatgtcccg gcgcaacaca cgtgagaaga aatcgatgac aggcgagagg tcctcgagtc
     4201 ccgggtagtg cagcgttacc cagtggcggg ctgcttcttc ggctgaatcg taggtagcct
     4261 cccagatggc gggatagata tgtacattcg catagatccc ctcgtcatgg aggatattca
     4321 gggggaactg gtagtcgg
//
LOCUS       DAOQVJ010000068         4325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22723_length_4325_cov_3.598361, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000068 DAOQVJ010000000
VERSION     DAOQVJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22723_length_4325_cov_3.598361"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKL26_02330"
     CDS             complement(<1..449)
                     /locus_tag="PKL26_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNS82563.1"
                     /translation="MRRKPIRLRDFIEDQDGWLYAVSTYDNQERAGCLLRYVPDSDGD
                     RVNAEGMHYRKVDFEESFELVKREKPEYCDLLHRVPLEDIRRVLKPEEELPHIAARDH
                     RIYSLVSLFNLPARTIGCTGSFLCGLENPGSDIDLVVYGRQWFHAQEL"
     gene            complement(455..1030)
                     /locus_tag="PKL26_02335"
     CDS             complement(455..1030)
                     /locus_tag="PKL26_02335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNS82564.1"
                     /translation="MDPVERAVGILRQDGLVVYPTDTIYGLGADALSEEAILKVYEAK
                     RRPIAQPISVAVSDLEMAFGIARIDDLARAFMEAFFPGPVTVVVPSRSSLPEILCGGT
                     GQVGIRLPAHPVAIRLIKLLDSPITATSANLHGARDPVTPDECHVMHDLLIDGGKLPG
                     TPSTVVDLEQCLILRRGALADAVDAYLETLR"
     gene            complement(1032..1712)
                     /locus_tag="PKL26_02340"
     CDS             complement(1032..1712)
                     /locus_tag="PKL26_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HNS82565.1"
                     /translation="MDEQESDLSALRIIAENRRGVLRDISTVVAGHNANILMIHQEIF
                     DSGPWDGMAELYIEFDACSDSAAMMSHLGEIPSVREVCSVEPFSHIYGTRVIIIGGGA
                     QVAQVALGAVNEADRHNIRGERISVDTIPLVGEKTVAQAVDAVARLPRAAILVLAGSL
                     MGGEISKAVDRVREAGIPVIALKMAGSVPQHVDMVVTDPIQAGVFAVMHVSEKAVFDI
                     NRVRGKEF"
     gene            complement(1721..1791)
                     /locus_tag="PKL26_02345"
     tRNA            complement(1721..1791)
                     /locus_tag="PKL26_02345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1757..1759),aa:Gly,seq:ccc)
     gene            complement(1921..2844)
                     /gene="aglJ"
                     /locus_tag="PKL26_02350"
     CDS             complement(1921..2844)
                     /gene="aglJ"
                     /locus_tag="PKL26_02350"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HNS82566.1"
                     /translation="MPIEKDQVCVLIPTLNEGPTIGALVREFRALGYTHIFVIDGNST
                     DDTRTIAAREGAGVEVQSGKGKGNALIEAFPLIEQPYILIVDGDGTYSPADAEKMLAP
                     LAEGYDQVIGDRLTDKNRDAFSRLNYFGNQVLNRLFKLAHSAYLSDILSGYRAFTRDA
                     IQQMHLHEAGFGIETEISAEAVRSGQKISVVPIEYKKRPGTPTKLNPFHDGFKITATI
                     YRLARMSNPLFYFGLIGLILMIAGVFLGIYVILEWLRQIEHLPLTIFTVLLIVIGFQI
                     FMFGVLSDMMLALHREVVRELHETRERKESR"
     gene            2883..3440
                     /locus_tag="PKL26_02355"
     CDS             2883..3440
                     /locus_tag="PKL26_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HNS82567.1"
                     /translation="MHIAKKGLRVLGIAESFSSREYSILAGVVMRKDLRVDGAALSRI
                     TVGGNDATEGVLAIYRELHRKDINVILLSGCVIAWFNILDPASIHRETGVPVVVVTYE
                     DSDGLSDDIRRHFPGDATRLARYESLGERAPLSLPTGHTAYIRPFGLPMEDANRLCRD
                     LTLDGKVPEPLRVARLVARAAMRFF"
     gene            complement(3517..4056)
                     /locus_tag="PKL26_02360"
     CDS             complement(3517..4056)
                     /locus_tag="PKL26_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNS82568.1"
                     /translation="MNHFTYLVIACMALSIMVAGCVQPSTTPSIPVPTSVTPVTPLPS
                     ATPLTDPALLGTWYLKAMTGPGGSSPVQTISPQMDATFSGQGDISGFAGCNHYSGQYI
                     LTGEALPNGWGISVGPVLSTLMYCAGTSDIEARFLSSLQNATSYSVTGDTLTITDHHG
                     GALVFQRTAYGPTAVPRGL"
     gene            complement(4146..>4325)
                     /locus_tag="PKL26_02365"
     CDS             complement(4146..>4325)
                     /locus_tag="PKL26_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HNS82569.1"
                     /translation="HHYGIQTIAGLGCRDTRWNPNEKIAIDFTDGTFHPGDLITVEVY
                     QRPKSTLISRHSFTA"
BASE COUNT          940 a         1212 c         1236 g          937 t
ORIGIN      
        1 agttcctggg catggaacca ctgccggccg tacaccacaa gatcgatatc ggacccggga
       61 ttctccaggc cgcagagaaa cgagccggta cacccgatgg ttcgcgccgg gaggttgaac
      121 aacgatacga gagaatagat ccggtgatcg cgtgcggcga tatgtggcag ctcctcctca
      181 ggtttcaaaa cccgccggat atcttccagg ggaacacggt ggaggaggtc gcaatactcc
      241 ggcttctccc tcttgacaag ttcgaacgac tcctcgaagt ccaccttccg gtaatgcatg
      301 ccttcggcat tcacccggtc tccgtcagag tcaggcacgt accggaggag acagcccgcc
      361 cgttcctggt tatcgtaggt ggaaacggcg tacagccacc cgtcctgatc ctcgataaaa
      421 tcacgaagac ggatgggttt tctcctcatc gctctcaccg caatgtctcg agatatgcat
      481 ctacggcgtc agcgagagca ccacggcgga gaatgagaca ttgttccagg tcgacaaccg
      541 tgctgggcgt cccgggcagc ttcccgccgt caatgaggag atcgtgcatc acgtggcatt
      601 cgtctggtgt gacagggtcc cttgcaccat gcaggtttgc actcgtggcc gtgatgggag
      661 agtcgaggag cttgatgaga cggatggcca caggatgtgc gggcagccgg atcccgacct
      721 gccctgtacc gccgcaaaga atctccggga gcgaggacct ggacgggacc accacggtca
      781 ccggtcccgg gaagaacgcc tccatgaacg cacgtgccag gtcgtcgatc ctagcaatgc
      841 cgaatgccat ctccaggtca gagaccgcga cagagatggg ctgggcaatg gggcgtcttt
      901 tcgcctcgta aaccttgagg atagcctcct cggagagggc atccgccccg agcccgtaaa
      961 tggtatcggt cgggtacacc acgagcccgt cttgccgaag gataccaacc gctctctcaa
     1021 ccgggtccat ctcaaaattc ctttcccctg acccggttga tgtcaaacac cgccttttcg
     1081 ctgacatgca tcactgcaaa taccccggcc tggattggat cggtaaccac catgtccacg
     1141 tgctggggaa cgcttcccgc catcttcagt gcaatcaccg ggatacctgc ctctctgaca
     1201 cgatccacag ctttggagat ctcgcctccc atcagggacc cggcgagcac gaggattgca
     1261 gccctcggga gccgtgcgac tgcatcaacc gcctgggcaa cggtcttttc acccacaagt
     1321 ggaatagtgt ccacagagat tcgctctccc cgaatgttgt ggcggtccgc ttcgttcacc
     1381 gctcccaggg caacctgtgc cacctgggcg cctccaccga taatgatgac tctcgtccca
     1441 tagatgtggc tgaacggttc gacggagcag acctctctta ccgatgggat ctcccccaaa
     1501 tgtgacatca ttgccgcgga atcggagcag gcatcgaact ctatgtagag ctcggccatt
     1561 ccatcccatg gaccggaatc gaagatctcc tggtggatca tcaggatatt tgcattgtga
     1621 ccggcgacta cagtagagat atcgcgcaga acccctcttc tgttctccgc gatgatgcgc
     1681 agggcagaga gatcggactc ctgttcatcc atggcgggta tgcgatagcc ggggatcgaa
     1741 cccgggttag aggcttggga agcccctgtc ctgccgctag actactatcg cacgagaaat
     1801 atggtagata ttattcgcgt ctgtccgaga taaaggttct tttttcagtg gaagaggggg
     1861 aaagagagca atgtgcgata tcatcgagga agggagcatc tcatgccaaa tcccttcgga
     1921 tcaacgcgat tcttttcttt cacgagtctc atgcagttct ctcaccacct cgcggtggag
     1981 ggccagcatc atgtccgaga ggacgccaaa catgaatatc tggaaaccga taacgataag
     2041 aaggaccgtg aagattgtca gcggcaggtg ctctatctga cgcagccact cgagtatgac
     2101 gtaaatgccg aggaagacac cggcaatcat gaggatcagg ccgataagac cgaagtagaa
     2161 gagcgggttg ctcatccgtg ccaggcggta gatggtggcg gtgatcttga acccatcgtg
     2221 gaaagggttc agcttggtgg gtgtccccgg cctttttttg tattcgattg gaacaacaga
     2281 gatcttctgc ccgctcctga ccgcctcagc cgatatctct gtctctatcc caaagcccgc
     2341 ttcatgaaga tgcatctgct gtatcgcgtc ccgggtgaat gcccggtagc ctgaaaggat
     2401 atctgagaga taggctgagt gagccagctt gaaaaggcgg ttgagcacct ggtttccaaa
     2461 ataattcagc cgggagaatg catcccggtt cttgtcggtc aacctgtcac cgatcacctg
     2521 gtcgtacccc tcggccagcg gtgcgagcat cttctccgca tcggcagggg agtatgttcc
     2581 gtctccatcg actatcagga tatagggctg ctcgatcagc ggaaacgcct cgatgagtgc
     2641 atttcccttt cccttccctg actgcacctc gacccctgca ccttctctgg ctgcgatggt
     2701 gcgggtatcg tctgtgctgt ttccgtcgat gacaaagata tgggtatagc cgagcgccct
     2761 gaactcgcga accagggccc cgatggtagg accctcgttc agggtgggga tgaggacgca
     2821 gacctggtcc ttctcgatgg gcatcgagct atatttaaaa atccattgac ataagtgctt
     2881 ccatgcacat cgccaagaaa ggcctgcggg tgcttggtat cgcggagagc ttctcctcac
     2941 gcgagtattc gatacttgca ggtgtggtga tgagaaagga ccttcgtgtc gatggagcag
     3001 ccctctcgcg tatcactgtg ggtgggaacg acgccacgga gggagtactg gctatatacc
     3061 gggaacttca tcgaaaggat atcaatgtca tcctgctgag cgggtgcgtg attgcatggt
     3121 tcaatatcct tgaccctgca tccatccaca gggaaaccgg agtcccggtc gtggtcgtca
     3181 cttacgagga ctcggatggg ctttcggatg atatccgccg ccactttccc ggagatgcga
     3241 cccgccttgc ccgttacgaa tcgctgggtg agagagcccc actcagcctc cctaccgggc
     3301 acaccgctta tatccgcccg ttcggcctcc ccatggagga tgcaaaccgg ctctgccggg
     3361 atctcacgct ggatggaaaa gtccccgaac cacttcgcgt ggcaaggctc gtggcaaggg
     3421 ctgcaatgcg gttcttttga taggtgtgca cgcgggaccc gcaatcccgg tcaagcttgc
     3481 aggagttatt cgaaaatccg ggcgcaaaaa aaaaagtcag aggcctcgcg gcaccgcagt
     3541 ggggccgtag gcagtcctct ggaatacgag ggcgcctcca tggtggtcgg tgatggtaag
     3601 tgtgtccccg gtgacagagt aactcgttgc gttctgcagt gaggagagga accgggcttc
     3661 aatatcgctt gtcccggcac agtacatcaa tgtggacaat accggcccta ccgaaatgcc
     3721 ccatccattg gggagtgctt ctcccgtgag aatatattgc ccgctgtaat ggttgcaccc
     3781 cgcaaagccc gatatatccc cctgtccgct gaatgtcgcg tccatctggg ggctgatggt
     3841 ctggacaggt gaagaaccgc cagggccagt cattgccttg agataccatg tcccgagaag
     3901 ggcaggatcc gtcaggggag tcgcagaggg gaggggggtc accggagtca cgctggttgg
     3961 cacggggatt gatggcgtgg ttgacggctg tacacaccct gcgaccataa ttgaaagggc
     4021 catgcaggca atcaccaggt acgtgaaatg gttcatatac gcataggagt gttttacggt
     4081 gaaagagata tcgctggtgg agcgccatgt ttcatatttt tttctcggct ccgcaacatg
     4141 tgacatcagg cagtaaagct gtggcgtgag ataagggttg atttcggccg ctggtatact
     4201 tcgacggtta tcaggtctcc tgggtgaaac gtcccatccg tgaaatctat agcgatcttt
     4261 tcgttcggat tccaacgcgt atcccggcat ccaagccccg caatagtctg tatgccataa
     4321 tgatg
//
LOCUS       DAOQVJ010000069         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_22841_length_4308_cov_3.928286, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000069 DAOQVJ010000000
VERSION     DAOQVJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22841_length_4308_cov_3.928286"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..595)
                     /locus_tag="PKL26_02370"
     CDS             complement(152..595)
                     /locus_tag="PKL26_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82570.1"
                     /translation="MIEYWIVVTTGVAAVAIVLCGAYLYNEIKSREFVQNRVKLKVYV
                     ALLQAVTEINVAGNDAYALDRAKRNLASTLNQINLIAGPQVLLHVNELLDFLNETREG
                     DYDVLKEKNILNSIVKAARRELDPASARALEESQFRFRFYNPPRP"
     gene            complement(676..1881)
                     /locus_tag="PKL26_02375"
     CDS             complement(676..1881)
                     /locus_tag="PKL26_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNS82571.1"
                     /translation="MKIIKDPVHGYVEVPSSMVSLLDSAPMQRLRYVRQLGFSYLVYP
                     GAHHTRFEHSLGTMHLAGVMARQLDLDADERLLVAAAGLLHDIGHGPFSHAIEPLSEE
                     FAAKSHQDVEGLLEQPETGGILERMGVDPDGVTSMIKGEHPLSGIIHGELDVDRMDYL
                     LRDAHYTGAPYGTVDAHRLIRSTTLADGRVVLEEAGINAAESLLIARTLMRPAVYFHH
                     VSRIAEMMFQGAVMAHVESGAKNSLLSLLHDDDAACMQALLTSPSPVAREISRRIYQR
                     RLYKRAIYVNRNQVNAARVAQCSPYPKRAALAAKIAAVAGLSPHEVLVDIPPFPGDMS
                     LHVQVRNRHRAVGISELSPLLHTLNETRRGQWRLGVYTLPEHRDEVAGIATDVLHVRP
                     ETTQERLFG"
     gene            1933..2835
                     /gene="cofD"
                     /locus_tag="PKL26_02380"
     CDS             1933..2835
                     /gene="cofD"
                     /locus_tag="PKL26_02380"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNS82572.1"
                     /translation="MITFLSGGTGTPKLVRGMRRHVPDHEISVVVNTAEDLWMSGNFL
                     SPDIDTLIYLFSGLLNTTSWWGQEGDTFTTHDELTRLDSGEYVAIGDRDRAIHILRGE
                     LLRRGMNLTRATAHLCASLKVRAQVLPMTNTQVETIIMTGGREIHFQEYWVRHRGTLP
                     VDEVKRRWETPPAATPEVLSAINSAEAVIIGPSNPITSILPILECGGVVEALESVPVV
                     AVSPFIGDAPISGPAAALMRARGLAPDSAATRDLYARFLDFFVQDTRDTVGVPGATRM
                     DTLMNREGIDEALAAGILSLVRGM"
     gene            2916..4178
                     /locus_tag="PKL26_02385"
     CDS             2916..4178
                     /locus_tag="PKL26_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS82573.1"
                     /translation="MSIFRSLKGALGGDSAHQMDEVEREHVESVRRALAYGDVEARWN
                     AIRAAGELGDAFIDPLVNALKDEHWIVRRGAGETLARIGLPAVPPLIEMLGSEDESVR
                     QEAMRACARAGEPAVDSLIRALSRENPAIRRGAAEILGEMQVQAATGPLIETLKDSDP
                     GVRREAAAALKLLGSEEAIAPLISLLGDESGYVRISAAEALCSLGGRSIDPLVAALAH
                     PQPEIRQRAGLALASIGTLAVEPLIAALNHGDSAVRQGAAGVLGRIGDPRAIPGLTRV
                     LGDKERKVRQEAVRALAALGYPAITPLVAAFREGDVVVRNCTMEALWLIGGPATRPLL
                     ELLSDQNSEVRRRTALLLGEIGDAAAVDGLTRLLSDQVPSVRREGFEALEKIRKKQGE
                     GSLGAGPAPRQPDQDPEKPGEGRKPGSW"
BASE COUNT          820 a         1324 c         1409 g          755 t
ORIGIN      
        1 tgttcccgca tctgctcgat gaattcacgc attgcagtct tgcctcccac ccatgcgata
       61 aggtgtgagc tgcactctga taaatctaca agcccccgaa acctgcctgg gaacgcaccg
      121 ccgggatgaa aaaagaaata ggacgggact ctcagggccg gggcgggttg tagaaacgga
      181 accggaactg ggactcttca agcgcccgtg cgctcgcagg gtcaagttcc ctcctcgccg
      241 ccttcacgat ggagttcagg atgttcttct ccttgaggac atcgtaatcg ccttcccgcg
      301 tctcgttcag gaagtcaagc agctcgttga cgtggaggag cacctgcggc ccggcgatca
      361 ggttgatctg gttcagcgtg gatgcaaggt tccgttttgc ccgatcgagc gcgtatgcat
      421 cgtttccggc gacgttgatc tcggtcaccg cctgcaggag tgccacgtat accttcagct
      481 tcaccctgtt ctgcacgaac tcccgcgatt ttatctcatt gtacagatat gcgccgcaga
      541 ggacgattgc caccgcggcc acgccggtgg tgaccacgat ccagtattcg atcaaagagg
      601 accccctcgc tctttcttgg cggcaggcgt tcttaaagat tctttttcgc tctctcgcat
      661 ccattgaaga gaggcctaac cgaatagcct ctcctgcgtg gtctcgggcc tgacgtggag
      721 cacgtctgtt gcgatgcccg cgacctcgtc cctgtgctcg gggagggtat agaccccgag
      781 ccgccactgg cccctgcggg tctcgttcag ggtgtgaagg agcggggaga gttccgatat
      841 gccgacagcc ctgtgacggt tcctgacctg gacgtgaagc gacatgtcgc cagggaacgg
      901 cgggatgtcc accagcacct cgtgggggga gagcccggca acggccgcga tctttgcagc
      961 cagcgccgct ctcttcggat acggggagca ctgggcaact ctcgccgcgt tcacctggtt
     1021 gcggttcacg tagatcgccc gcttgtagag cctgcgctgg tagatccggc gggagatctc
     1081 ccgcgcgacc ggcgaggggg aggtgagaag agcctgcata caggcagcat cgtcatcgtg
     1141 gaggagcgaa agaaggctgt ttttggcacc actctcgacg tgcgccatca ccgccccctg
     1201 gaacatcatc tccgcgatcc ggctcacatg gtggaagtag accgcggggc gcatcagggt
     1261 gcgggcaata aggagcgact cggccgcatt gatcccggcc tcctccagga ccaccctgcc
     1321 atctgcaagg gtggtgctcc ggatcagccg gtgggcatcc accgtgccgt acggggcccc
     1381 ggtatagtgc gcgtcccgca ggaggtaatc catccggtcc acgtcaagct cgccgtggat
     1441 gatacccgag aggggatgct cccccttgat catggaggtc accccgtcag gatcgacacc
     1501 catccgttcc aggatccccc cggtctccgg ctgctccagg agtccttcga cgtcctggtg
     1561 gctcttcgct gcgaactcct ccgagagcgg ctcgattgcg tgagagaagg gcccatgccc
     1621 tatgtcgtga aggagccccg ccgcggccac aagaaggcgc tcatccgcgt caaggtcgag
     1681 ctgtcgggcc atcacccctg caaggtgcat cgtcccgagg gagtgctcga acctggtgtg
     1741 gtgggcacca gggtagacaa ggtacgagaa cccgagctgc cgcacgtacc ggagcctctg
     1801 catgggagcc gagtcaagga gggagaccat ggacgacgga acttccacgt agccatgcac
     1861 agggtccttt atgatcttca tgcccaatcc gaaacaacac tcttctcacc ctcctataaa
     1921 tagtatagaa gtatgatcac cttcctttcc gggggaaccg ggacccccaa gctggtccgg
     1981 ggcatgaggc ggcatgttcc cgaccacgag atctccgtcg ttgtcaacac cgccgaggac
     2041 ctgtggatga gcgggaactt cctgtcgccc gacatcgata ccctgatcta cctcttctcg
     2101 ggactgctca acaccacgtc gtggtggggg caggaggggg acacgttcac cacccacgat
     2161 gaactgacgc gcctggacag cggtgaatac gtcgccatag gcgaccgcga ccgggccatc
     2221 catatcctgc ggggagaact cctgaggagg gggatgaacc tcaccagagc gaccgcgcac
     2281 ctgtgcgcgt ccctcaaggt cagggcacag gtcctgccca tgacgaatac ccaggtcgag
     2341 accatcatca tgacgggtgg gcgcgaaatt cacttccagg agtactgggt caggcacagg
     2401 ggaacgctcc ctgtcgacga ggtaaagagg agatgggaga cgcctcccgc ggccaccccc
     2461 gaggtcctct ccgcaatcaa ttccgcggag gcggtgatta tcgggcccag caatccaatc
     2521 accagcatcc tgcctatcct cgaatgcggg ggagttgtgg aagccctgga gagcgtgcca
     2581 gtggtcgccg tgagcccttt catcggtgac gcgccgataa gcgggcctgc cgccgcattg
     2641 atgagggcaa gggggcttgc ccccgattct gccgcaacga gggatctcta cgccaggttc
     2701 ctggacttct tcgtacagga cacgcgggac acggtgggcg ttccgggcgc gacccggatg
     2761 gacacgctga tgaaccgcga ggggatcgac gaggcacttg cggcagggat cctctccctt
     2821 gtacggggga tgtgaaaggc cggttttgct gcccccggca acaaaaaggg ttttttacct
     2881 gcgcggcaga cacaggtgtg aggaagggga cccgaatgag catctttcga tcactcaagg
     2941 gagcgcttgg cggggatagc gcgcaccaga tggacgaggt ggagagggag cacgtggaga
     3001 gcgtgaggag ggcgctcgct tatggggacg tggaggcccg ctggaacgcg attcgggcgg
     3061 caggtgaact cggggacgcc tttatcgatc cgcttgtcaa tgccctgaaa gacgagcact
     3121 ggatcgtccg gagaggtgca ggggagaccc ttgccaggat cggcctccct gccgtgcccc
     3181 ctctcatcga gatgctggga agcgaagacg agtcggtccg ccaggaggcc atgcgtgcat
     3241 gtgcacgtgc aggggagcct gccgtggact cgctgatacg tgccctctcc cgtgagaacc
     3301 ccgcgatacg ccgcggtgcc gccgagatac tgggggagat gcaggtccag gccgcaaccg
     3361 gaccgctgat cgagacgctg aaggacagcg accctggcgt gcggcgggag gcggcggcgg
     3421 ccctcaagct gcttgggagc gaggaggcga tcgccccgct cataagcctt ctcggcgacg
     3481 agtccggcta tgtccgcatc tccgcggccg aggcgctctg ttccctggga ggaaggagca
     3541 tcgaccccct tgtggcggct ctcgcccacc cgcagcccga gatccgtcag cgtgcggggc
     3601 ttgcgctcgc ctcgatcggc accctcgcgg tggaaccgct catcgcggca ctcaaccacg
     3661 gggactcggc ggtcaggcaa ggcgcagcgg gagtgctcgg gaggatcggg gacccgcggg
     3721 ccatacccgg gctgactcgg gtgctcggcg ataaggagag gaaggtccgc caggaggcgg
     3781 tcagggcgct tgctgcgctc gggtatcccg caatcacccc cctcgtggca gcgttccggg
     3841 agggtgacgt ggtggtgcgg aactgcacca tggaagcgct ctggctcata ggggggccgg
     3901 ccacccgccc gcttctcgag ctcctctcag accagaactc agaggtccgc aggaggaccg
     3961 cgctccttct cggggagata ggcgacgcgg ctgcagtcga cgggctcacc aggctcctgt
     4021 ccgaccaggt gccatcggtc cggagagagg gattcgaggc gcttgagaag ataaggaaga
     4081 agcagggcga gggatcgctc ggtgccgggc ctgccccccg gcaaccagac caggacccgg
     4141 agaagccggg cgaggggagg aagcctggct cctggtgagg aaaatccagg ggactatcag
     4201 ggacgaagcg taaaccggga ggcgataagt gatgcccgga cctcgatccc ctgcaccctc
     4261 acaaggtatt cgtccggcac ccatccccgg gtatcgattg taaaggac
//
LOCUS       DAOQVJ010000070         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_23037_length_4281_cov_3.964742, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000070 DAOQVJ010000000
VERSION     DAOQVJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23037_length_4281_cov_3.964742"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PKL26_02390"
     CDS             complement(<1..1156)
                     /locus_tag="PKL26_02390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNS82574.1"
                     /translation="MTLADDLARQQRSISVAEFFEKNKHLLGFDSPTRGIITTIKEAV
                     DNALDACEEAMVLPDIFVGIKRTESDVFRITVEDNGPGIVPAQVPFVFGKLLYGSRFH
                     QIRQSRGQQGIGISAAVLYAQLTTGLPTIVTSRTGPENPAFRFFIQINIKTNEPEVQE
                     QSEVEWDRTHGTRVQIEFKSTMAAKKRLLEYLKYTSVVNPHARIRVELDDETYLFERV
                     SDEVIACPQAIKPHPHGIEIGQLMRMLGAAEGSLGEFLIESFTRVGKKTAEEMCRSAE
                     ISPGTLASSLSPIEQKRLLSAMQGVKVPAPPTQTCLSPIGEDLITRGLEKEFQMDFVR
                     ARTRPSSVYSGHPFLVEAAIGYGGKLPPDGNAQLLRFANRVPLLYQQGACA"
     gene            complement(1143..2375)
                     /gene="hisS"
                     /locus_tag="PKL26_02395"
     CDS             complement(1143..2375)
                     /gene="hisS"
                     /locus_tag="PKL26_02395"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNS82575.1"
                     /translation="MLQKPRGTRDFLPDEMESRREVERVMREVARTWGYREICTPEFE
                     ELEIFTIRSGEGIIQEMYVFEDKGGRKMALRPELTASVLRMFVNEGRTLPKPLRWFYF
                     ADCFRYERPQKGRYRQFWQFGVELIGADSALAEAEVIMLAHEILSASGISFSLHVGHL
                     SFMRSLLAELEPDDQKRARMFLDKKDWRGLEDYLSSRGESGLSERLISLAECRDLREV
                     FSITGENPERKRIEDLFCVLDQSRIPYILNPGIARGLDYYTGMVFEAFAEGLGAENQV
                     LGGGAYRLAHLFGGEDTPSCGFAAGFDRVMVARGARPLSKGVSVGVAFTPEGREWALA
                     AAKEFRNIGIRTHLDLSGRSIGQQLSHISRTADYALVIGSRERDERSVTLKDLRAGTQ
                     KMLSLPDAVSEVSGADSR"
     gene            complement(2386..2724)
                     /locus_tag="PKL26_02400"
     CDS             complement(2386..2724)
                     /locus_tag="PKL26_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82576.1"
                     /translation="MSYLIYVAAFGTLAVFFLWLRDVRIFLRTGLPGYRNAAYRGVLH
                     GALAILGCAFTFSSTERFPTELIGLGIILAALYFQGKVTRERVFTKESTWDRVAGNAP
                     ARKRTAEPEE"
     gene            2774..3550
                     /gene="larB"
                     /locus_tag="PKL26_02405"
     CDS             2774..3550
                     /gene="larB"
                     /locus_tag="PKL26_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNS82577.1"
                     /translation="MSPNHRVREVLSLFREGQISLDDAVDDICGLRIEAVAALANLDT
                     GREARCGVPEVVLAEGKSPSQVGEIARNVTAAAGRIIFSRLSPDHVEALIDVCQDCGF
                     SLTLHEQAAMAVVSTGPPTEPGKGVVAIITAGTSDIRVAEEARVIASEMGCRVKTAYD
                     VGAAGIHRLFPALRECVGAHVYIVAAGREGTLPAVVAGLVDRPVIGVPVGTGYGYMGR
                     GRAALASMLQSCSVIAVVNIDAGFVAGAFAAQIANMVGER"
     gene            3547..4074
                     /locus_tag="PKL26_02410"
     CDS             3547..4074
                     /locus_tag="PKL26_02410"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HNS82578.1"
                     /translation="MKRGFYIGRFQPYHNGHQYILEKIAKTADEIIIGVGSAQLSHEP
                     ANPFTAGERVLMITRALKHIGCPLYVIPIEDIRRNALWVAHVRSMVPPFYTVYSGNPL
                     VVRLFEEEGIRVESPAMYERDSHSGTEIRKRILEDGDWESLVPDAVVQVIREIDGVRR
                     IRQIARSDGDGREPW"
     gene            4081..>4281
                     /locus_tag="PKL26_02415"
     CDS             4081..>4281
                     /locus_tag="PKL26_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82579.1"
                     /translation="MLRKVKDLFGKKEAPVVQEIRLDDIPALISDHEPVWEAELVQAA
                     SASRKKILSTRDSLLAAVRRLSE"
BASE COUNT          847 a         1229 c         1310 g          895 t
ORIGIN      
        1 tggcacatgc cccctgctga tagaggaggg ggaccctgtt tgcgaaccgc agtagctgtg
       61 cgtttccgtc gggaggaagt tttccgccat aaccgatcgc ggcttccacg aggaaaggat
      121 ggccggagta gaccgaactc ggccgtgtcc tcgcccgcac gaagtccatc tggaactcct
      181 tctcaagtcc cctcgtgatc aggtcttcgc cgatggggga gaggcacgtc tgggtgggag
      241 gggcagggac ttttaccccc tgcatggcag agagcagcct cttttgttcg ataggggaga
      301 gcgatgaggc cagggtgcca ggagagattt ctgcgctccg gcacatctcc tcggcggtct
      361 tcttccccac ccgtgtgaaa ctctcgatta ggaattcgcc gaggcttcct tcggctgccc
      421 cgagcatccg catcagctgg cctatctcga ttccgtgcgg gtgaggcttg atcgcctgcg
      481 gacatgcgat cacctcgtcg ctgacgcgct cgaagaggta ggtctcatca tcgagttcca
      541 cgcgtatgcg ggcatggggg ttgacaacgg atgtatactt gaggtactcg agcagcctct
      601 tcttggcggc catggtgctc ttgaactcga tctgcactcg tgttccatgg gtcctgtccc
      661 actcgacttc gctctgttcc tgcacctcgg gctcgttggt cttgatattg atctggatga
      721 agaaccggaa ggcaggattc tccgggccgg tgcgggaggt cacgatagtg ggaaggccgg
      781 tagtgagctg tgcatagagt actgcggcac tgataccaat cccctgctgc cccctgctct
      841 ggcgtatctg gtggaaccgc gagccgtaaa ggagttttcc aaacacgaaa ggtacctgtg
      901 cggggacgat cccgggaccg ttgtcctcga cggtaatcct gaatacgtcg ctttcagtcc
      961 gcttgatgcc cacaaagatg tcggggagga ccatggcctc ctcgcaggcg tccagggcat
     1021 tgtcaacggc ctccttgatg gtggtgatga tgccccgtgt cggggagtca aaccccagga
     1081 gatgtttgtt cttttcaaag aactctgcta cgctgatgct cctctgctgg cgtgcaaggt
     1141 catcagcgag agtcagcgcc actcacctca gagactgcgt cggggagtga aagcatcttc
     1201 tgcgtcccgg ccctgagatc tttcaacgtg acggaccgct cgtcacgctc ccgcgaaccg
     1261 atgaccagtg catagtcggc agtccgggag atgtgcgaga gctgttgccc gatggatctc
     1321 cccgagaggt cgagatgcgt gcggatgccg atgttgcgga actcctttgc cgcggcaagg
     1381 gcccattccc tcccttcggg cgtgaacgcc acccctacag acacgccctt tgagagggga
     1441 cgcgccccac gggctaccat aacacggtcg aaccctgccg caaacccgca ggagggggtg
     1501 tcctctccac caaagagatg ggcaagccgg taagcgcccc ccccgaggac ctggttctct
     1561 gcgccgagtc cctcggcgaa tgcctcgaat accatcccgg tgtagtagtc gaggccgcgg
     1621 gcaatgcctg ggttcaggat ataggggatc ctggactggt cgagtacgca aaagaggtct
     1681 tctatcctct ttctttcggg gttctcgccg gttattgaga atacctccct gaggtctcgg
     1741 cattctgcaa gtgaaatgag tctctcagag agcccggact cgccccggga agagaggtaa
     1801 tcctcgaggc cgcgccagtc cttcttgtca agaaacatcc gcgctctctt ctggtcatcg
     1861 ggctcaagct cagcgagaag tgacctcatg aacgagaggt ggccgacatg gagggagaat
     1921 gaaataccgg acgcagagag gatctcgtgg gcgagcatga tgacctccgc ctccgcaagc
     1981 gctgaatcgg caccgatcag ctctaccccg aactgccaga actggcggta gcggcctttc
     2041 tggggacgct cgtaccggaa acaatcggcg aagtaaaacc acctgagagg tttgggaagc
     2101 gtcctccctt catttacgaa cattctgagt acagacgccg tcagttcggg ccgaagcgcc
     2161 atcttcctgc cccccttatc ttcgaagacg tacatctcct ggataatccc ctctcccgag
     2221 cggatggtaa agatttcaag ttcctcgaac tccggcgtgc agatctcgcg atacccccag
     2281 gtacgggcaa cttcccgcat cacgcgttcg acttcacgcc gcgattccat ctcgtcaggg
     2341 agaaaatccc gcgttcctcg tggtttctga agcatggaaa agacctcact cctccggttc
     2401 cgctgttctc ttcctggccg gggcattccc tgccacgcgg tcccaggtgc tttcctttgt
     2461 gaatacacgc tccctcgtga cctttccctg gaagtagagg gctgcaagga tgatcccaag
     2521 gccaatcagc tctgtaggga aacgctccgt tgaggagaac gtaaacgcac acccgaggat
     2581 ggccaatgcc ccatggagca ccccgcggta cgcggcattc cgatatccgg ggagccctgt
     2641 gcgcaggaag atcctcacgt cacggagcca caggaagaat actgccagcg ttccgaacgc
     2701 ggcaacgtag atcaggtaac tcatgcagtt gcatcactta ttatacccag gcccatatct
     2761 taattatcac agcatgtccc cgaatcaccg agtcagggag gtcctctccc ttttcaggga
     2821 gggccagatc tccctcgacg atgcggtcga tgatatctgc ggccttcgca tagaggctgt
     2881 tgccgccctc gcgaaccttg atacaggaag ggaagcccga tgtggggtac ccgaggtggt
     2941 gctggcggaa ggaaagtccc cgtcccaggt gggagagatc gcacggaatg tgactgccgc
     3001 tgccgggagg atcatcttct cccggttgtc tccggaccac gtcgaggctc ttattgatgt
     3061 ctgccaggac tgcggctttt ccctgactct tcacgaacag gcggccatgg cagtggtctc
     3121 cactggtcca cccacagaac ccgggaaggg ggtggtcgcg attatcactg ccggtacatc
     3181 ggatatcagg gtggcggaag aggccagggt catcgcgtcc gagatgggat gtcgtgtgaa
     3241 gacggcatat gacgtgggag ctgccgggat tcaccgcctc ttccccgctc tccgcgaatg
     3301 cgttggtgct catgtataca ttgttgcggc cgggagggag gggacccttc ccgcggtggt
     3361 cgcaggtctg gtagaccgcc ccgtcattgg ggttccggtc ggcacggggt atgggtacat
     3421 gggcaggggc agggctgcgc ttgccagcat gctccagtcg tgctcggtca ttgcagtcgt
     3481 caacatcgac gcgggctttg tggccggggc atttgcagcc cagattgcga acatggtggg
     3541 agagagatga agcgcgggtt ttacattggg agattccagc cgtatcacaa cggtcaccag
     3601 tacatccttg agaagatcgc aaagactgca gacgagatca tcatcggtgt cggcagcgcc
     3661 cagctctcgc acgagcctgc aaatccgttc actgcgggag agcgggtgct gatgatcacg
     3721 cgggcgctca agcatatcgg ctgtccgctc tatgtcatcc ctattgagga tatcaggcgc
     3781 aatgcgctct gggttgcgca cgtgcgatcg atggtcccgc cgttttacac cgtgtactcg
     3841 ggcaatccac tggtggtccg cctctttgaa gaggagggaa tacgtgtcga gtcgccggcc
     3901 atgtacgagc gtgattcaca cagcgggacc gagatcagga aaaggatact tgaagatggc
     3961 gattgggaga gcctcgtccc ggatgcggtt gtgcaggtga ttcgcgagat tgacggggta
     4021 aggaggatca ggcagatagc gaggagcgat ggggacggca gggaaccgtg gtgatatggc
     4081 atgctccgca aggtgaaaga cctcttcggc aagaaggaag cgcctgtcgt gcaggaaatc
     4141 aggctggatg atatcccggc cctcatctcc gatcatgaac cggtgtggga ggcggagctt
     4201 gtgcaggcag catccgcctc aaggaagaag atcctctcga cacgggacag tcttctggcc
     4261 gctgtccggc ggctttccga g
//
LOCUS       DAOQVJ010000071         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_23099_length_4271_cov_4.796727, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000071 DAOQVJ010000000
VERSION     DAOQVJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23099_length_4271_cov_4.796727"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKL26_02420"
     CDS             complement(<1..494)
                     /locus_tag="PKL26_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82580.1"
                     /translation="MQKTARMIYPVLAAMLLLFAVFSGCVTEIPDSCNLTEGELADRY
                     LAQAATIRDLQSEYVVYSGMAAGDLRVEKISFYFKTPSFTRIEMEPETAGEQGSFATS
                     SGTSTSWYDSRTRVYDLYSGMNLSREYDYQRMVRHIIADRDFAILERDTEHGASRYLI
                     EVVT"
     gene            774..962
                     /locus_tag="PKL26_02425"
     CDS             774..962
                     /locus_tag="PKL26_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82581.1"
                     /translation="MIKNSARPTVIGTRSGYTIRFTCPNCAKENSIVYNMPKAFYKES
                     RDAVCGKCRKHFTVLTPD"
     gene            complement(1062..2576)
                     /locus_tag="PKL26_02430"
     CDS             complement(1062..2576)
                     /locus_tag="PKL26_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNS82582.1"
                     /translation="MNRILSYSLIVLFLIAAATLAAGCISEKDAGASSTVDEPAAKAA
                     AEYEASNYRSAAELYMQAYSDYQARGDTVSARKAMREALVSQRMIFEFPFNRSAVESE
                     IIQAYPEFSAREIADLLSEEKSIRIVSDGEHLYFSDTVSNIMFHNLTLARRMTKEFNH
                     SPFYDETCSLAFATPLPNKGPYGEPVVFEITQRLLLPTVLLPETGTMRIWVPAPIESG
                     SQSDVAILSIEPAQYVRSWPDTSADIGLAYLEIPLADVKGDLLNITTRFRFTQHEVRF
                     VIDPSRVKAYNLSNPEYLQYTMSEKNIAVTQEIKKKAHEIVGDETNPHLQAEKIYWHI
                     MNTLPYSHVPHFMLETAGIPESTYVLETGFGDCGSQSMYFAALCRSLGIPARSTGGFQ
                     LVPGLAGEHFWAEYYLEGYGWIPVDVTVAESADWSHDATDEDRHRYKEYYFGSLDPYR
                     YIIQKNVDAPLTPDPGDAVMFRLAVQNPKIACDTCTTDMEILLVEYWTTNVTQG"
     gene            2762..3151
                     /locus_tag="PKL26_02435"
     CDS             2762..3151
                     /locus_tag="PKL26_02435"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNS82583.1"
                     /translation="MKVGIIRCLMTEDLCPGTMDFRVTAEGKGAFADTGPVEIVGFVT
                     CGGCPGKRAIPRAKMLVERGAQAIVFASCITRGNPIGYPCPHYADMRDCVKKAIGPDV
                     FHHRMDALTRLIARQPQIGSSPNRSIQ"
     gene            complement(3109..3420)
                     /locus_tag="PKL26_02440"
     CDS             complement(3109..3420)
                     /locus_tag="PKL26_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNS82584.1"
                     /translation="MAKIAAELVRDAADLLGIRDQASLHEIRALYHDRVREWHPDVSR
                     KDPVESHEMTIRLREAYELLVEYCMNHPFSFRAEDLARELADAPADYWMERFGDDPIW
                     G"
     gene            3525..>4271
                     /locus_tag="PKL26_02445"
     CDS             3525..>4271
                     /locus_tag="PKL26_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS82585.1"
                     /translation="MAEDRPLIYLNNAATSWPKPPEVLAAISESLSLPVFGSGRTTVS
                     QGEDYVTKARDALSGLFGVETAEHVVFTQNATDSLNVLIQGFLERHAGDCHVLTTALD
                     HNSVLRPLHEYERQGRIRLTVLPFEDGRVSPAAVTEAITPMTRLMVTTHGSNVLGTAA
                     DIRGIGEVLHDHGAYFVVDGAQAAGHVPIDFESLPVDAYAFTGHKALLGIAGTGGFVI
                     KDPEAIAPVRFGGTGTDSFSLLHPAGMPERF"
BASE COUNT          916 a         1223 c         1200 g          932 t
ORIGIN      
        1 tcggtcacga cctcgataag gtaccgggaa gccccgtgtt ccgtgtctcg ctcaagtatc
       61 gcaaaatcac ggtccgcgat gatatgccgg accatccgct gatagtcata ctcgcgggag
      121 aggttcatcc cggaatagag atcatagaca cgggttcggg agtcgtacca cgaagtggat
      181 gtgccgctgg aggtcgcaaa cgatccctgc tctccagcag tttcaggctc catctcaatc
      241 cgcgtaaagg aaggcgtctt gaagtagaac gagattttct ccactcgcag atcccctgcg
      301 gccattcccg aatacacaac gtattccgac tgcaaatccc tgattgttgc ggcctgggca
      361 aggtagcgat cggcaagctc gccttcagtc aggttgcagg agtcagggat ctccgtgaca
      421 cacccggaaa agaccgcgaa gagaaggagc atcgcggcaa ggacaggata tatcatccgg
      481 gctgtcttct gcatggtatc cccgttcctc aaaacggcac gataagtgtt tcgctttatg
      541 gtttcagatc ccctccttct ccccatccca tggttattta cccttgcacg gcctatgata
      601 atattccaga caaaccctgg attccgggag cgccttttga ttggacacgg gaatttcata
      661 tccgccccgt atcgtttgcc ccgggcatac cgcatgtatc ggctgtcaga acgaaagatg
      721 aatgccgtgg atcatcaatc ctgttgctcc acgcatcaca cagacaggat tagatgataa
      781 agaacagcgc aagacccacg gtaatcggga ccaggtcagg gtataccata cgctttacct
      841 gtcccaattg tgcaaaagag aactcaatcg tatacaatat gcccaaggcc ttctacaagg
      901 aatcaaggga cgcagtgtgc gggaagtgca ggaagcactt caccgtgctt acccctgatt
      961 gatatacaga aacggcgggg agaagaggag agattcttcc ttttccctcc cttttctccc
     1021 tcctcaaaaa cccctttcct gcgtgattct gaacgaactc ctcatccctg tgtgacattc
     1081 gtcgtccagt actctacaag caggatttcc atgtccgtgg tacacgtatc gcaggcaatc
     1141 ttcgggttct gcactgccag gcggaacatc actgcgtcac cgggatcagg ggtgagagga
     1201 gcatccacgt ttttctggat gatatacctg tagggatcga ggcttccgaa gtaatactcc
     1261 ttgtagcggt ggcgatcctc atcagtcgca tcatgggacc agtctgccga ttctgcaacc
     1321 gtgacgtcaa ccggtatcca gccatacccc tccaggtaat attctgccca gaagtgttca
     1381 cccgcaagcc ccgggaccag ctggaaccct cccgtggacc gtgcggggat accgagtgat
     1441 cggcacaggg cagcaaaata catgctctgc gatccgcagt cgccgaaccc agtctcaagg
     1501 acataggtgg attcggggat ccctgcagtc tccagcatga agtgcggaac gtggctgtag
     1561 ggaagtgtgt tcatgatatg ccagtagatc ttctccgcct ggaggtgtgg gttcgtctcg
     1621 tcaccgacaa tctcgtgtgc tttcttcttt atctcctgtg tgacggcaat gttcttttca
     1681 gacatcgtat actgcaggta ttcgggattg ctcaggttgt aggccttcac cctggaaggg
     1741 tcaatgacaa accgtacctc gtgctgggta aatcggaacc tggtcgtaat gttgaggaga
     1801 tcgcccttta catccgcgag cgggatctcc aggtacgcaa gcccgatgtc ggctgaggta
     1861 tccggccagg aacgtacgta ctgtgccggc tcgatcgaga ggatcgcaac atccgattgt
     1921 gaaccggact ctatgggggc cgggacccat atccgcatgg tgccggtctc cgggagaagt
     1981 actgttggaa gcaagaggcg ctgggttatt tcaaatacga ccggttcgcc gtagggcccc
     2041 ttgttgggaa gcggagtggc gaacgcaagg ctgcacgtct cgtcatagaa aggtgaatgg
     2101 ttgaactctt ttgtcattct ccgggccagt gtgagattat gaaacatgat attgctgact
     2161 gtatcggaga aatagaggtg ttcgccgtca ctcactatgc ggatactctt ctcttccgag
     2221 aggagatcgg cgatctccct ggctgaaaat tccggatacg cctggattat ctctgactcg
     2281 actgcggacc ggttgaacgg gaactcgaag atcattcgct gcgatacgag tgcctccctc
     2341 atggccttcc gggcagagac agtgtcaccc ctggcctgat aatcgctgta tgcctgcatg
     2401 tagagttcag cggcagaacg gtaattcgat gcctcgtact ccgcggcggc ctttgcggcg
     2461 ggttcgtcaa ctgtggagga ggccccggca tccttttccg atatgcagcc tgccgcaagc
     2521 gtcgcggcag cgataaggaa caggactatg agggaatatg ataagattcg attcatgcat
     2581 caccttgcgt tgaggatcta ttgaataggg aactgagcgg aagtgacttt aagctgatgt
     2641 gtgaaaagtt gcgaaaattc tcctttccgg cctgaactgg gtggatcctg catcaaggtg
     2701 aattctggtc ttctgtacaa atccaaacgc ttatcgcccg aaaccgctca acgcctcttt
     2761 tatgaaggtg ggaattatcc gctgcctgat gaccgaggac ctctgcccgg ggacgatgga
     2821 ctttcgcgtg actgccgagg gtaagggtgc gtttgcagat actggcccgg tcgagatcgt
     2881 cggcttcgtg acgtgcgggg ggtgcccggg caaacgggcc atcccgcgtg caaagatgct
     2941 cgtggagagg ggtgcgcagg cgatcgtgtt cgcctcgtgc atcaccaggg ggaacccgat
     3001 cgggtacccg tgcccgcact atgccgacat gagggactgc gtgaagaaag ccatcggccc
     3061 ggatgttttc catcatcgaa tggacgcact gacgcggcta atcgcgcgtc agccccagat
     3121 cgggtcgtct ccgaaccgct ccatccagta gtccgcgggg gcatctgcaa gttcccgtgc
     3181 gaggtcttca gccctgaaag agaacgggtg gttcatgcag tactctacga ggagttcata
     3241 cgcctccctg agccggatgg tcatctcgtg cgactcgaca gggtctttcc gggagacatc
     3301 cgggtgccac tccctgaccc gatcgtggta gagcgcacgg atctcgtgaa ggctcgcctg
     3361 atccctgatc ccgaggaggt cagccgcgtc cctcaccaat tcggccgcaa ttttggccat
     3421 tgcatactgg tttcgggagc gccgggataa tggttgccac cgctgctgca gaaccgatca
     3481 ctttttcgtc accctcgcga gaacttcgag tgagaacgag tgccatggca gaagaccggc
     3541 ccctcatcta cctgaataac gccgccacca gctggccaaa acctcccgaa gtcctcgcag
     3601 cgatatcgga atccctctcg ctcccggtct ttgggtcggg gcggaccacg gtgtcccagg
     3661 gcgaggatta tgtcacgaag gcccgcgacg cactttcggg cctctttgga gtggaaaccg
     3721 ccgagcacgt ggtcttcacc cagaacgcga ccgactccct gaacgtcctc atccagggat
     3781 tcctcgaacg ccacgcgggt gactgccacg tccttaccac agcgctcgac cataactctg
     3841 tcctccgccc tctccacgag tacgagcgcc aggggaggat ccgcctgact gtcctcccct
     3901 tcgaagacgg ccgtgtcagc cccgctgcgg tcacagaggc aatcaccccc atgacccggc
     3961 tgatggtcac gacgcacggg agcaacgtgc tcgggaccgc ggcggacatc cggggaatcg
     4021 gagaggtgtt acacgatcac ggagcctatt tcgtagtcga cggagcccag gcagccgggc
     4081 atgtacccat cgactttgag agcctccctg tcgatgccta tgccttcacc ggccacaagg
     4141 cactcctcgg catcgcaggg accgggggct ttgtgataaa agaccctgag gcaatcgcac
     4201 cggtcaggtt cgggggaacc gggaccgatt ccttctcgct cctccacccc gcagggatgc
     4261 cggagcggtt c
//
LOCUS       DAOQVJ010000072         4207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_23614_length_4207_cov_3.931118, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000072 DAOQVJ010000000
VERSION     DAOQVJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23614_length_4207_cov_3.931118"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="lonB"
                     /locus_tag="PKL26_02450"
     CDS             <1..1176
                     /gene="lonB"
                     /locus_tag="PKL26_02450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HNS82586.1"
                     /translation="NLLTALQEGEFPITGQSERSSGAMVRTEPVPCKFIMIAAGNLDA
                     IQGMHPALRSRIRGYGYEVYMSESMLDTPENREKFIRFIAQEVKNDGKIPHFDKGAME
                     EIIREARRRSNRKGHLTLKLRDMGGLIRVAGDLARQQGAEVTTADHVIAAKKTARSIE
                     DQVSDEYIHRSREYELTVVEGTRVGRVNGLAVMGSDSGSVLPIMAEVTPAQGASGTVI
                     ATGMLKEIAQESIKNVSAILKKFTGKDIKNMDVHIQFIGTYGGVEGDSASISVATAVI
                     SAIESIPVRQDIGMTGSLSVRGDVLPVGGVTYKIEAAAKAGIKTVLIPRANIEDVLIE
                     ERYQSMVTIIPVDHIEDVLKIALVPENREGFLNKLRKMASTPSGSFLDTTGIPTSPA"
     gene            1181..>2025
                     /locus_tag="PKL26_02455"
     CDS             1181..>2025
                     /locus_tag="PKL26_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNS82587.1"
                     /translation="MARARYFDVRHVTGTVTHVDIDNTRVESAGTSFLDRAVLRVLGP
                     RGWGILAIDHYTRPKGRRFDELVERGAGLASLTGQEVLLADFPSESIRVPPMKENPAT
                     VELDEKQRLLADIERSARLEGIKSTRANYIERFEEVQFEDSSGNEYSCAVCRSGFSIL
                     AVADRSGDLQMGYVRKHTLQDFNLRHRQDDGLMAAERAIALLDARPPRGGTMNAVLDP
                     ELAGVFAHEAVGHASEADLVQEGSSVLAGRIGERIGSPAVTIVDDPSLPEFGFEPFDA
                     EGCRVG"
     assembly_gap    2026..2125
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2126..2389
                     /locus_tag="PKL26_02460"
     CDS             <2126..2389
                     /locus_tag="PKL26_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNS82588.1"
                     /translation="QVDPGRGVFQFNAEYGYLIKDGEIGEMVRDVSLSGEILMTLHSI
                     SLCAGDRIMHQGYCGKGGQTVPVSDGAPHVLLRDAVVGGSGTD"
     gene            2376..3659
                     /locus_tag="PKL26_02465"
     CDS             2376..3659
                     /locus_tag="PKL26_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNS82589.1"
                     /translation="MELIDRILKEGEKRVDELEVYFFSGSAVTVRLRKKEIHYASGSD
                     HQGLSLRVINEGRIGASSTSNPERWEGCLDAAIASARLATPQDWKGLPGPRDLDFTPL
                     NFDPAVIPDADTAGTLVQGLLEGAAAYPVEITSGSAEVARSHAVLANSSGIRYHENES
                     MVSVGVETIVGQSTGYEFDVSCRMDIDPQKVGERAAYLAYSSQGGKDIGSGHYDVVLS
                     PVALSQFLGSVFVPALSGKNVHAGRSRLASLLDQEIMDGDLSLLDDPFHPRGLSSCRW
                     DAEGIPTRKVKFVDGGVLREFAYDLKTAYRYGMESTGSAVRGGHGGSPSIGVHTLTLD
                     GLRNDLLDGAVVHVHDVVGAHTANPLSGDFSVELSNALFMRDGNVEFPVRKAMLSGNV
                     FEMLKETGGLSQDERVVGSMVLPEIRLKNLAIIGN"
     gene            3662..3928
                     /locus_tag="PKL26_02470"
     CDS             3662..3928
                     /locus_tag="PKL26_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HNS82590.1"
                     /translation="MNLDFAVLIAGIIIAVALFFLIKDITKLIINSILGLLILFFANL
                     FNIMGSLGRTDIPYSLVNILLCILGGIPGALIVIVLHLLGIEGF"
BASE COUNT          865 a         1186 c         1276 g          780 t
ORIGIN      
        1 aacctcctca ccgccctcca ggagggggag ttccccatca ccgggcagag cgagcgctcc
       61 agcggggcca tggtccggac cgagcccgtg ccatgcaagt tcatcatgat cgcggcagga
      121 aatcttgacg ctatccaggg gatgcatcct gcgctccgct cgcgtatccg cgggtatgga
      181 tacgaagtct acatgagcga gagcatgctg gacacgcccg agaaccggga gaagttcatc
      241 cggttcattg cacaggaagt caagaacgac ggcaagatcc cccatttcga caagggcgca
      301 atggaggaga ttatcaggga ggcccgcagg cgttccaacc ggaagggtca cctcactctc
      361 aagttaaggg acatgggcgg cctcatccgc gtagcagggg acctggcgcg gcagcagggt
      421 gccgaggtga cgactgccga ccacgtaatc gctgccaaga agactgcaag gtctatagag
      481 gaccaggtct cggacgagta tatccaccgg agcagggaat acgagcttac cgtggtcgaa
      541 gggacaagag tcggccgcgt gaacggcctc gcggtgatgg ggagtgattc gggctcggtg
      601 ctccccatca tggcagaggt cacgccggct cagggagcca gcggcacggt gattgcaaca
      661 gggatgctga aggagatcgc ccaggagtcc atcaagaacg tgagcgcgat cctgaagaag
      721 ttcaccggga aggacatcaa gaatatggat gtccatatcc agttcatcgg gacctacggc
      781 ggcgttgaag gtgattccgc atccatcagc gttgcgaccg cggttatcag cgcaatagag
      841 agcattcccg tgcgacagga catcggcatg accggatccc tttcggtccg cggtgacgtg
      901 cttcctgtag ggggagtgac ctacaaaata gaggcggctg caaaggccgg catcaagact
      961 gtgctcatcc cccgggcaaa tatcgaggat gtcctgatcg aggaacggta ccagtcaatg
     1021 gtcaccatca tcccggtcga ccacatcgag gacgtcctga agatcgcgct ggtgcccgag
     1081 aaccgcgaag ggttcctgaa caagctgcgg aagatggcat cgaccccttc aggatctttc
     1141 ctggacacga cagggatccc aacctccccg gcgtgaagcc atggcacgtg cccgctattt
     1201 tgatgtccgg cacgtgacgg gcaccgtcac ccacgtggac atcgacaaca cccgcgtgga
     1261 gtcggcaggc acgtccttcc tggacagggc cgttctccgg gtgctcggcc cccggggatg
     1321 gggcatcctt gcaatcgatc attacacccg gccgaaaggg aggcggttcg atgaacttgt
     1381 tgagaggggc gcaggccttg catctctcac gggacaggag gtgctgctcg ccgattttcc
     1441 ctccgaaagt atcagagtcc cgcccatgaa agagaaccct gccaccgttg agcttgatga
     1501 gaaacagagg ctcctggctg atatcgagcg aagtgcacgc ctcgagggga tcaaaagtac
     1561 cagggccaat tacatcgagc ggttcgagga ggtccaattc gaggacagca gcgggaacga
     1621 atactcctgc gccgtctgca ggtcgggctt ctccatcctc gccgtcgcgg accggtcggg
     1681 ggacctgcag atggggtacg tgcggaagca caccttgcag gacttcaacc tgcgccatcg
     1741 ccaggatgac ggcctgatgg ctgcagaaag ggcaattgca cttctcgatg cccgtccgcc
     1801 gcgggggggc acgatgaatg cggtgctgga ccccgagctt gccggagtat ttgcacacga
     1861 ggcggtgggc cacgcaagcg aggctgacct cgtgcaggag ggcagctcgg tgctcgcagg
     1921 gagaatagga gagcgtatag ggagcccggc agtcaccata gtggatgacc cctcgctccc
     1981 cgagttcggg ttcgagccct tcgacgctga agggtgcagg gtgggnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnncaggt ggatcccggg aggggcgtct tccagttcaa
     2161 tgcggaatat ggatacctga taaaggacgg cgagatcggg gagatggtga gggatgtctc
     2221 cctttcaggc gagatcctca tgaccctgca ctcgatctca ctctgtgcag gagaccgcat
     2281 catgcaccag ggatattgcg gcaagggcgg gcagacggtg ccggtcagcg acggggcacc
     2341 gcacgtgctc ctgcgggacg cggtggtggg cggcagtgga actgattgac cgtatattaa
     2401 aagaaggcga gaagagagtc gatgaactgg aggtctactt tttctctggg tccgcagtca
     2461 ccgtccggct caggaaaaag gagattcact atgcttccgg atcggaccac cagggactct
     2521 ctctccgggt tatcaacgaa ggcagaatcg gcgcatcttc gaccagcaat cccgaacgat
     2581 gggagggctg ccttgatgcc gcgatagcca gcgcccgcct cgcgactccg caggattgga
     2641 aagggctgcc cggtccccgc gatctcgatt tcaccccgct gaacttcgac cctgcggtca
     2701 tccccgatgc agacaccgcc ggcacgctgg tgcaggggct cctcgaaggt gcggccgcct
     2761 acccggtaga gatcacatcg ggaagcgccg aggtcgcaag gtcgcacgca gtgctggcca
     2821 acagcagcgg tatccgatac cacgaaaatg agagcatggt gagcgtgggt gtggagacga
     2881 ttgtcggcca gtccacggga tacgagttcg acgtgtcctg caggatggat atcgatccgc
     2941 agaaggtggg tgagcgggca gcttacctgg cctactcctc acagggagga aaggatatcg
     3001 gcagcgggca ctacgacgtg gtcttgtccc cggtcgccct ctcacagttc cttggaagcg
     3061 tgttcgtccc ggcgctgagc ggaaagaatg tccatgccgg ccgttcaagg cttgcaagcc
     3121 tccttgacca ggagatcatg gatggcgatc tctcgctcct cgacgacccg ttccacccaa
     3181 gaggcctctc gagttgcagg tgggatgccg agggaattcc cacgagaaag gtgaaattcg
     3241 tggatggggg agtgctccgc gagtttgcct atgacctgaa gaccgcgtac cggtatggaa
     3301 tggagagcac aggaagtgcg gtcaggggag ggcacggggg gtccccctcg atcggcgtcc
     3361 acactctcac cctcgacggg ctgaggaacg atctccttga tggcgcggtg gtccacgtac
     3421 atgacgtggt gggtgcccac accgcaaacc cgctctccgg tgacttctcc gtggaactct
     3481 cgaatgccct cttcatgcgg gacgggaatg ttgaattccc ggtcaggaaa gccatgctct
     3541 cagggaacgt atttgaaatg ctcaaggaga ccggcgggct ctcgcaggac gagagggtgg
     3601 tgggatcgat ggtcctgcct gagataagat taaaaaacct ggcgatcatt ggtaattagg
     3661 catgaaccta gatttcgcag tcctgattgc cggaatcatc atcgctgtgg cgctcttctt
     3721 tctcatcaag gacatcacga agctgatcat caactcgatc cttggcctcc tgatcctctt
     3781 ctttgcaaat ctcttcaaca tcatgggcag cctcgggagg acggacatcc cctattccct
     3841 cgtgaacata ctcctctgca tcctgggggg catccctgga gcccttatcg tgatcgtgct
     3901 ccacctgctg gggatcgagg gattctagga agggataatt tcccttcagc cctgccgtcc
     3961 cttaaaaaat gcacttccag cctttacttg cacagccgat cgattgactt cttcgggcct
     4021 gttcctgcgc ataccccccg cagcagcggg cctttcagcc tattcccgca tcagccgatc
     4081 gattgacttc ttccgggtct gttcctgcgc ataccccccg cagcagcggg ctttttgccc
     4141 gctgctgctc cttcaaaaca cgttcgcttc cgtatacacc agcacctggc cctgctggat
     4201 ctcgtac
//
LOCUS       DAOQVJ010000073         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_23793_length_4183_cov_5.053295, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000073 DAOQVJ010000000
VERSION     DAOQVJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23793_length_4183_cov_5.053295"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKL26_02475"
     CDS             <1..490
                     /locus_tag="PKL26_02475"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HNS82591.1"
                     /translation="IAAGAASIFGISLEILPVIILLILLAAYDAISVYKTKHMIALAE
                     GVVETKSPILFVVPKKRGYSYRKEGLGPLEEGERGAFIIGMGDMIMPAILVVSANVFF
                     QGAKLGFLTIPALGAMAGSVAGLSVLLVYVNRGKPQAGLPPINGGAILGFLAGYGLAL
                     AL"
     gene            complement(458..1414)
                     /locus_tag="PKL26_02480"
     CDS             complement(458..1414)
                     /locus_tag="PKL26_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNS82592.1"
                     /translation="MEFETIPTVPTADEILDRSFRRAASKMKLKKNKDRANEEFVRAV
                     SQAIHDRLVKVIQSFPDFDAVSPFYRDIVDIMWGLDRVRKALGGVGWAARWARTHGPG
                     LAYQTRKSENPSQIRKRAVARLSSVVHQVEEDLLFLNEVRNVVRKLPHVSDEFTVVVA
                     GYPNVGKSSFIRLVSTAVPEVASYPFTTKGIIVGHRAVGKERMQFIDTPGVLDRPSDE
                     RSGIERQALTAMVNLASVILVIIDASEHCGYPLEDQFRLLHEIEEMVSVPVKAVVNKS
                     DLAYFDGYINMSTETGEGVDEVLESLLAYRKRSEGESQAVPG"
     gene            1462..2340
                     /locus_tag="PKL26_02485"
     CDS             1462..2340
                     /locus_tag="PKL26_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82593.1"
                     /translation="MDLSLIQKDILITLITLYHQHSHPIKGEEIAEVIKRNPGTVRNQ
                     MQALKAIGFVDGIPGPKGGYNPTAQAYRALNLDISGKEYDVKIARNGEEVQGAKVVEI
                     AFTTLCHPDICRANATLIGSMKAFGIGDQITIGPTPVNKLLIRGEVYGKDEVSQTLLI
                     SIFEMISLPKKPIRYYMSSPLISLCRADPLKNALHLFNSHHIRGAPVVEKDELFGIVT
                     MSDVANAIEKNVPMDTPVSTIMTPDVVFAPSSVQLFEVIRSFKERDIGRLIVMEEGRP
                     VGILTQSDIIKVFPSL"
     gene            2472..2723
                     /locus_tag="PKL26_02490"
     CDS             2472..2723
                     /locus_tag="PKL26_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS82594.1"
                     /translation="MSRVLARSLSKKKIVTNEGKVIGTLKNLVVDFETGQVVDLVIYP
                     DPSFDTSGYRTEGDRLYIPFEAVKDIKDYIVVDRYLSRK"
     gene            complement(2705..3418)
                     /locus_tag="PKL26_02495"
     CDS             complement(2705..3418)
                     /locus_tag="PKL26_02495"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNS82595.1"
                     /translation="MNRSFCLKGLVTDIDGTITDPRRRIHTGAIEILRDLMEKGVYVV
                     LASGNTSCFMDAVSKMIGTPGTYIGENGGIIRNGYGQDIALLGDGAAPRKALCDLVEA
                     YQRKGISIEHYSLPYRFVDVAFARTLPVDEVRGILEDHPVEVLDTGFAIHIHPPGVNK
                     GVAFSELARAMGLDTRDFLAVGDAENDIELLRRAGIGVAVANAHPQLVLYADHVTERE
                     CGDGFIEGVKKYMAYFLER"
     gene            complement(3993..>4183)
                     /gene="radA"
                     /locus_tag="PKL26_02500"
     CDS             complement(3993..>4183)
                     /gene="radA"
                     /locus_tag="PKL26_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328658.1"
                     /note="Involved in DNA repair and in homologous
                     recombination. Binds and assemble on single-stranded DNA
                     to form a nucleoprotein filament. Hydrolyzes ATP in a
                     ssDNA-dependent manner and promotes DNA strand exchange
                     between homologous DNA molecules; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HNS82596.1"
                     /translation="PAVFFGDPTKPIGGNIVGHTATFRIYLRKSKGGKRIARLVDSPN
                     LPEGEAAFMVEEAGLKPV"
BASE COUNT          923 a         1152 c         1160 g          948 t
ORIGIN      
        1 catcgccgca ggggctgcct ccatcttcgg gatatcgctt gagatcctcc cggtaatcat
       61 ccttttgata ctccttgcgg cttacgacgc catatccgta tacaagacaa agcacatgat
      121 cgcccttgcc gaaggggtag tggagaccaa aagccccatt ctcttcgtgg tcccaaaaaa
      181 acgcggatac agctacagga aggaaggact cgggccgctt gaggaaggtg aaaggggtgc
      241 gttcatcatc gggatgggag acatgatcat gccagccata ctcgtcgtct cggcaaacgt
      301 ctttttccag ggagcaaagc ttggcttcct gactattcct gcgcttggcg cgatggcagg
      361 ctcggttgcc gggctctctg tcctgctggt ctatgtcaac cgcggaaaac cgcaggcagg
      421 cctccctcca ataaatgggg gagcgatcct tggattctta gccgggtacg gcctggctct
      481 cgccctctga tcgcttccgg tatgcaagca gggattccag cacctcgtcc accccttcac
      541 cggtttctgt cgacatgttg atgtatccgt caaagtacgc gagatccgac ttgttcacca
      601 ctgccttgac aggtacgctg accatctcct cgatctcgtg gagcagcctg aactggtcct
      661 ccaggggata cccgcagtgc tcgcttgcat cgatgatgac cagtattacc gaggccaggt
      721 tcaccatggc ggtgagggcc tggcgctcta tgccgcttcg ctcatcagac gggcggtcga
      781 gcacaccggg agtatcgatg aactgcatgc gttcctttcc gacagcccgg tggccgacaa
      841 tgatgccctt ggtggtgaac ggataggagg caacttcagg aactgctgtg gagacgagcc
      901 tgatgaacga ggatttgccg acgttcgggt atcctgcaac cactactgtg aactcatcgc
      961 tgacatgggg gagtttgcgg acgacgttcc tgacctcgtt caaaaagagc aggtcttctt
     1021 cgacctggtg caccaccgat gagagcctgg ccacggctct cttccgtatc tgcgatggat
     1081 tctccgattt cctggtctgg taggcaaggc ccgggccgtg ggtgcgggcc cacctggcgg
     1141 cccatcccac cccgccaagg gccttccgga cgcgatcaag cccccacatg atatccacga
     1201 tatcccggta gaatggagag accgcatcaa aatcggggaa agactgtatc accttgacca
     1261 ggcggtcatg gattgcctgg ctcacggccc ggacgaattc ctcgtttgcc cggtccttgt
     1321 tcttcttgag tttcatcttg gatgccgccc ggcggaaact acgatcgaga atctcgtcag
     1381 cggtgggcac cgttggaatg gtctcaaatt ccactgtggt tcaacctaac ttatatatgc
     1441 ccatgcagat gataaaccat tatggatttg tccctaatcc agaaagacat ccttatcacg
     1501 cttattacgc tgtatcacca gcattcgcat ccaataaaag gggaggagat cgcggaggtg
     1561 ataaagagga accctggaac ggtccggaac cagatgcagg cattaaaagc aattggcttt
     1621 gtggatggaa tacccgggcc aaagggggga tataatccaa ctgcccaggc atatcgtgcc
     1681 ctgaacctgg atatctcggg gaaagaatac gatgtgaaga tagccagaaa cggggaggag
     1741 gtacagggtg ccaaggtggt cgagatcgcg tttaccaccc tctgccaccc ggatatatgc
     1801 cgggcgaacg ccacgctgat aggctcaatg aaagcattcg ggatagggga ccagatcacg
     1861 atcgggccga ccccggtgaa caagctcctg atacgtggcg aggtgtatgg aaaggacgag
     1921 gtctctcaga cgttgctgat ctccatcttc gagatgatat ccctgccgaa aaagccgatc
     1981 aggtactaca tgagcagccc gcttatctcg ctttgcaggg cagatcccct gaaaaatgcg
     2041 ctccatctct tcaactcgca ccacatcagg ggtgcgccgg tggtcgagaa ggacgagctg
     2101 ttcgggatcg tgacaatgag cgacgtggca aacgcgatcg agaagaacgt cccgatggac
     2161 actcctgtct ctactatcat gacccccgat gtggtatttg ccccgtcctc ggttcagctc
     2221 ttcgaggtaa ttcggagttt caaggagcgt gatatcggcc ggctcatcgt catggaagag
     2281 ggacgcccgg ttggaatact cacgcaatcc gatatcatca aggtcttccc gtcactctag
     2341 gtacgatgcg ggttgcatgg accattgtgc cttttttcag gcacgcagaa gcgcatgaat
     2401 ttgtaccaga acggcgacta ttccggcgat tttaaacatg tttatatatg aatagggcca
     2461 cacccttttt catgtcacga gtcctggccc gtagtctttc caagaagaag attgtcacga
     2521 acgaggggaa ggtgattggc accctgaaga acctggtggt agatttcgag accgggcagg
     2581 tggttgacct ggttatatat cctgaccctt cgtttgatac ctcgggatac cgaaccgaag
     2641 gggaccgcct ctatatcccg ttcgaggcgg tgaaagatat caaggactat atcgtagtag
     2701 accgctacct ctccaggaag taggccatat acttttttac accctcgata aaaccgtcac
     2761 cacactctct ttctgttaca tggtctgcat agaggaccaa ttgcgggtgc gcattcgcga
     2821 cagccacacc gatgcctgca cgcctgagta gttcgatgtc attctctgca tcgcccacgg
     2881 caagaaaatc cctggtatca aggcccatgg cccgtgccag ctcggaaaac gccacaccct
     2941 tgtttacacc cggcggatgg atgtggatgg caaaccctgt atcaagcact tcgacgggat
     3001 gatcctcgag gatcccgcgg acttcatcga cagggagagt gcgggcaaat gccacgtcaa
     3061 cgaaacggta ggggaggctg taatgctcga tagatatgcc cttcctctgg tatgcctcaa
     3121 ccaggtcgca aagtgccttc cttggtgcgg ccccgtcccc aagaagggcg atgtcctgtc
     3181 cgtacccgtt ccttatgatg ccgccgttct caccgatgta ggtcccggga gtgccgatca
     3241 tcttggagac ggcatccatg aagcatgaag tattgccgct tgccagcact acatacacac
     3301 ctttctccat caggtcacgg aggatctcga tggctcccgt atggatcctc cgtcttgggt
     3361 cggtgatggt tccatcgata tcggtgacga ggcccttgag gcaaaaagac ctgttcattt
     3421 gtgtattccg gatatggact ctcgtcccgg gatccttatg cctttcggat ttgagaataa
     3481 ttgaacgagg gcttctttcc tgatttcccc cgaaggcctt gcctgtcgaa catatggtta
     3541 tatggcgatc acccggatcc tcggtgtttt ttcgcagacg atggggagac ggcgcgaggg
     3601 taccgccctt tgatcccggg gcgacctgga ggtcgcaatg attcgcggtt cttatgaggt
     3661 ggaagagata cccttgcatc aggttcaacc cccccaagat agccagggag cagggttaca
     3721 atgaaaccca gggaaaagac aatctgctcc catttcccat ctcccaacac tcgataatcc
     3781 atgggcaggg cagattcgca cggtggttga tctgcacctg ttccccggct ccggcctcac
     3841 cgccgtcagg ggttgcgcaa gcgtgaaggg ttcatcaatg ccaggcacgt tcccatggga
     3901 tgagtggtgc actgtgaagg tatgaaatca atatctctca catcatggcc tcccgaaggt
     3961 ttcatgccgg gaaaaagagt atggcttgag aattatacgg gcttgagtcc ggcctcttcc
     4021 accatgaatg ccgcctcgcc ctcggggagg ttggggctgt cgacgaggcg ggcgatgcgc
     4081 tttcctccct tgctcttccg caggtagatg cggaaggtcg cggtgtggcc cacgatgttt
     4141 ccgccaatgg gcttggtggg atccccgaag aagaccgcgg ggt
//
LOCUS       DAOQVJ010000074         4181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_23810_length_4181_cov_3.802957, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000074 DAOQVJ010000000
VERSION     DAOQVJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23810_length_4181_cov_3.802957"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PKL26_02505"
     CDS             <1..971
                     /locus_tag="PKL26_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNS82597.1"
                     /translation="MARIPESDVEAIKKVVGSGRVIPVLRSAEEMTYGDDSGYVTLLG
                     LPAGEIPGLLTVEKGLYLKENAPGCLVGYYLAREFGISPGSRVGIGNQTQRVSGILKE
                     RGYAADINPDYAIVVTDQWFFETFGDEKEYDQVIVKASDISQIDTLKKEIDQRLNRRD
                     KVVDIYDSRDLLRQYDQIYNQIMVFLLGIGAISLLVAAVSILNVMIISVTERIREVGI
                     LRSIGASRKDILLMFLCEAMMLGIIGSTIGGVLSVIGGYFISKVAIGVMTAGTTFGEN
                     ASVFGPGTLVFVAGGILFGVIVSTLSGLYPAWQASRMTPIEALRHE"
     gene            979..2181
                     /locus_tag="PKL26_02510"
     CDS             979..2181
                     /locus_tag="PKL26_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82598.1"
                     /translation="MAGILISLAMRNLQRHWVRSLLAGIGIMIGVFAIASLGLLGNSL
                     VILFSGMITDVGDTLVITPHLAAASGDPFDPRSAVGAGIAERDLARITKAAGPNQVIP
                     VVEAGERLEVRDRGGYVILLALKSEHLPLLLSVREGVFPRETGVGVLVGALLAEEYDI
                     APGHIINVGGEPARVVGVLEERGMAIDINPDYAVVVTHGWFADRHPDPGYTRVLVKVG
                     DIGSMAAVKEDIDFQVNRQRERFDILDSREILDLFYSTYDAITLFLLGIGGVALLVSG
                     VSILNVMIISVTERTKEIGILRSIGTLRGQVMRMFLYEALVIGSIGSILGGVLSIAGG
                     YYLSVVVADTLTSSFSTVPDVRVFDLQGIGYIVLGVFFGILVSCLSGLYPAVRAVRIR
                     PIEALRYE"
     gene            2258..3427
                     /locus_tag="PKL26_02515"
     CDS             2258..3427
                     /locus_tag="PKL26_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82599.1"
                     /translation="MQRSLFFDFAVRNVRLHWFRSLLAVIGIIIGVVAIASMGILGNS
                     LVLAISDSLSTVGDSIIVTPHAGGGMGPSMGGSSELITDRKVEQIRRAVAPDLTIPVY
                     SGTARMRIGTEDLVGVVYALKPDDVPILLELEEGAFLRGSSGAMAGAKFAADNNLKVG
                     SRITIRDKGSLRVVGILAERGMGFDINPDYGIVVEDKWYQQAYDQKDYDMVIVKVKDL
                     TRIEQTKEAIEKQLNRRETEVDVIDTKAILETILTTFGQISTFTTAIGGISLIVAGVS
                     ILNIMMMSVTERIKEIGVLRSIGTMRKEVRSMFLFEALILGLIGSILGGVLSIFGGYA
                     VSIVMLQTTEYLFVPSSLVYIFYGMAFGVGTSLLSGLYPAWKASNLNPIEALRHE"
     gene            complement(3507..3773)
                     /locus_tag="PKL26_02520"
     CDS             complement(3507..3773)
                     /locus_tag="PKL26_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82600.1"
                     /translation="MMMSRELRIGPALKTGDRIVLALSRQTKMAWPGGCTGNCTPVAL
                     VIVEGEECTVWQLGGDAAMEEIKDTLAGIAESLTLDAPITRSCL"
     gene            complement(3770..>4181)
                     /locus_tag="PKL26_02525"
     CDS             complement(3770..>4181)
                     /locus_tag="PKL26_02525"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HNS82601.1"
                     /translation="SDRRPGDMALQEPQDLPASSYIQSAVRYVPDLISFVMAAVRSLS
                     FRRLDCRAVVGLSGPAETGMLYGYFWAAKPLLCSSGRINLEMVPDFTREYLEGHLELD
                     VNISYPFNLLARLVRLFTLFQENKTHCGKSGVPA"
BASE COUNT          881 a         1103 c         1305 g          892 t
ORIGIN      
        1 tcatggcaag gatccccgag tcggatgttg aggcgataaa aaaggtcgtg ggctcaggca
       61 gggtaatccc cgttctccga agtgctgagg agatgacata tggcgatgat tccggctatg
      121 tcacgcttct tggtcttcct gccggggaga tccccggcct cctcacggtt gaaaagggct
      181 tgtatctcaa agaaaacgcc cccgggtgcc tggtgggata ctacctggca agggagtttg
      241 gtataagccc ggggagccgg gtcggtatcg ggaatcagac ccagagagta tcaggaatac
      301 ttaaggagag gggatatgcc gccgatatca atccggacta tgcgatcgtc gttacggacc
      361 agtggttctt cgagacgttc ggagatgaga aggagtatga ccaggtgatc gtaaaagcga
      421 gcgacatctc ccagatcgac acgctcaaga aagagatcga ccagcgcctg aaccgtaggg
      481 acaaggtggt ggacatctat gattcacgtg acctgctgcg acaatacgac cagatttaca
      541 accagatcat ggtattcctc cttggtatcg gggctatatc ccttctcgtc gctgcggtga
      601 gcatcttaaa cgtgatgatc atctccgtga ccgagcgcat acgcgaggtc gggatcctca
      661 ggagcatcgg cgcatcgagg aaggacattc tcctgatgtt cctctgcgag gcgatgatgc
      721 tcgggattat cgggagcaca atcggggggg ttctttcggt gattggcgga tatttcatca
      781 gcaaggtggc catcggtgtg atgacagcgg ggaccacgtt cggagagaat gcttcggtat
      841 ttgggccggg gacactcgtc ttcgtcgccg gagggatcct cttcggggtg atcgtgagca
      901 cgctctcagg gctttaccct gcctggcagg cctcgcggat gacgccgatc gaggccctgc
      961 gccacgagtg atgcggtgat ggccggcatt ctcatctcac tggcaatgcg gaacctgcag
     1021 cggcactggg tgcgctcgct tcttgcgggc atcgggatca tgattggggt gtttgcaatc
     1081 gcatcgctcg ggctgctcgg aaacagcctc gtcatcctct tctcgggcat gatcacggat
     1141 gtcggcgata ccctcgtcat cacaccccac cttgccgcgg cgagcggtga cccgtttgat
     1201 ccccggtcag cggtgggcgc aggcatcgca gagcgcgacc tggcccgcat cacgaaggca
     1261 gcagggccaa accaggtgat cccggtggtc gaggccggcg agcggctcga agtgagagac
     1321 aggggaggct atgtaatcct gctggcattg aagtccgagc acctccctct gctcctgtct
     1381 gtgcgcgaag gagtgtttcc cagggagacc ggtgtcgggg tgctggtggg agcgctcctc
     1441 gcagaggagt acgacatcgc ccccgggcat atcatcaacg tcgggggcga gccagcgagg
     1501 gtggtgggag ttctcgaaga gaggggaatg gccatcgata tcaaccctga ttatgcggtg
     1561 gtggtcacgc atggctggtt cgctgaccgg catcccgatc ccggctatac ccgtgtcctc
     1621 gtgaaggtcg gggacatagg gagcatggca gcagtgaagg aggatattga tttccaggtg
     1681 aaccgccaga gggagaggtt cgatatcctt gacagcaggg agatcctcga cctgttctat
     1741 tccacctatg atgcaatcac cctcttcctg ctcggcatcg gcggagttgc cctgcttgtc
     1801 tccggggtca gcatcctgaa cgtgatgatc atctcagtca ctgagaggac aaaggagatt
     1861 ggaatcctcc ggagtatcgg cacgttgaga gggcaggtga tgcgaatgtt cctctacgaa
     1921 gcgctggtga tcggatctat cggaagtatc ctaggaggag tgctctcgat tgcggggggg
     1981 tattacctga gtgtggtggt tgcggacacg ctcacctctt ccttcagcac cgtgccggat
     2041 gtgagggttt ttgacctgca ggggatcgga tatatcgttc ttggtgtctt cttcggcatc
     2101 ctggtcagct gcctctcagg gctttacccc gcagtcaggg cagtccgtat aagaccgata
     2161 gaagcattgc gctatgaatg agaatgatat agcacggggg acataccact tatctggcag
     2221 aggagatcgg gcgagcgggt cggactggtg atgattagtg caaagaagct tattttttga
     2281 ttttgcggtc aggaacgtgc ggctccactg gttccgttca ctcctcgcgg tgatcgggat
     2341 catcatcggg gtggttgcca tcgcttccat ggggattctc ggcaacagcc tcgtgctcgc
     2401 aatctccgac agtctttcga cggtgggaga cagcatcatc gtaactcccc acgcaggagg
     2461 tggcatgggc ccgtccatgg gtggaagcag cgagctgatc accgatcgca aggtcgagca
     2521 gatcaggaga gcggttgcgc ctgatctcac tattccggtc tactctggga ctgcaaggat
     2581 gaggatcggg acggaggacc tggtgggggt ggtatatgcc ttaaaacctg acgacgtccc
     2641 cattctcctc gagctcgagg aaggagcatt cctgcggggg agctccgggg ccatggcagg
     2701 cgcgaagttt gccgccgaca acaacctgaa ggttgggtcc cgcatcacga tccgggacaa
     2761 gggctcactg cgggtggttg ggatcctcgc ggaacgggga atgggctttg acatcaaccc
     2821 cgactatggg atcgtggtgg aggataagtg gtaccagcag gcgtatgacc agaaggacta
     2881 cgacatggtc atcgtgaagg tcaaggacct cacccggatc gagcagacca aggaggccat
     2941 cgagaagcag ctgaaccgcc gggagaccga agtcgacgtg atcgatacca aggcaatcct
     3001 cgagaccatt ctcaccacgt ttgggcagat ctccacgttc acgacggcaa tcggcgggat
     3061 ctcccttatt gttgcggggg tgagcatcct gaatatcatg atgatgtcgg tcacggagcg
     3121 gatcaaggag atcggagtgt tgcggagcat cggcacgatg cgaaaggagg tccggagcat
     3181 gtttctcttc gaggccctga tcctcgggct tattgggagc atcctcggag gggtgctgag
     3241 catcttcgga ggatacgccg tgagcatcgt gatgctccag accacagagt acctgttcgt
     3301 gccgtcaagc cttgtctata tcttctatgg tatggcattc ggagtgggga cgagcctgct
     3361 gtcagggttg tacccggcat ggaaagcatc gaacctgaac ccgatagagg cattaagaca
     3421 cgagtaacca tttttttggg gacatggtat tttgcgaccg gacactgctt cagatccctg
     3481 aaaaatcgcc gatacaagga agaggatcac aggcacgatc gggtgattgg cgcatccaaa
     3541 gtgagagatt cggcaattcc cgcgagcgta tccttgatct cctccatggc ggcgtctcca
     3601 cccagttgcc acacggtgca ctcctcacct tccacgatca ccagggcaac cggcgtacag
     3661 ttccctgtac acccccccgg ccacgccatt tttgtctggc gggagagtgc aagcacgatc
     3721 ctgtctcccg ttttcaatgc cggccctatc cgcaactccc ggctcatcat catgcaggga
     3781 ccccgctctt cccgcagtgc gttttattct cctggaagag tgtgaagagg cgcaccagcc
     3841 tcgcaagaag gttgaaagga taggagatgt tgacgtccag ttcaaggtgt ccttcaagat
     3901 actcccttgt gaaatccggg accatttcca ggttgatcct ccccgaactg cagaggaggg
     3961 gttttgccgc ccagaaatat ccatagagca ttcccgtctc tgcaggacct gagaggccca
     4021 ccactgccct gcagtcaagc cgcctgaaag agagagaccg gacagcggcc ataacaaacg
     4081 agatcaggtc ggggacgtaa cggactgcgg attggatgta tgacgatgcc ggcaagtcct
     4141 gtggttcctg caaagccata tcccccggcc ggcggtcgct g
//
LOCUS       DAOQVJ010000075         4130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_24212_length_4130_cov_4.180859, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000075 DAOQVJ010000000
VERSION     DAOQVJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24212_length_4130_cov_4.180859"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            595..1797
                     /locus_tag="PKL26_02530"
     CDS             595..1797
                     /locus_tag="PKL26_02530"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNS82602.1"
                     /translation="MRRNIKIEALHQIPLERQEIELVERKCIGHPDSLADGIAESISR
                     ALSKAYLEECGAILHHNTDQGEIVAGESQPKYGGGKVIRPIYMLIDGRATKQFDGINI
                     PTDAIAVEAARTYLRETLTNLNMERDIIVDCRLGTGSTDLRDVFKPCQNTPPRANDTS
                     FGVGHAPFSETETIVKAVSDYIDGTFRPRYPAVGQDIKIMGLRDGDTITLTLGCAMVD
                     RYCSGLPEYLEYKDLLREHILNVARQHTGRKVIAAINTADDIDTGSVFLTVTGTSAEM
                     GDDGSVGRGNRCNGLITPNRPMSMEATSGKNPINHIGKIYNVISTQLAQECVAKVDGI
                     EEIYIRLLSQIGKPIDQPLVASVQILPGEGVDMKTISPDIEAIIDNGLAEITCITEKI
                     IRGEIKTF"
     gene            1775..2692
                     /gene="hisG"
                     /locus_tag="PKL26_02535"
     CDS             1775..2692
                     /gene="hisG"
                     /locus_tag="PKL26_02535"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNS82603.1"
                     /translation="MARSRRSEGEESPGPVARPQVLRLAIPNKGRISVPVNELMEKSG
                     LHLLDVGERRLVSRTPDPHVEILFARPVDIPEYVANGAADLGITGHDMVLERKSDVSE
                     LLDLKIGSASLVLAVREDAGYTNPLDLEGTRVATEFPVISKEYFQEKGISVTLVPVGG
                     ACEATPYLGIAEAIVDLSSSGNTLRSNRLRVIDTVLPTTTMLIANPQSLASKGGKIEE
                     IVLALESVVRARGQCYLMMNAKRACVDEVTSVLPGLSGPTVMEVASRQDMVAIHAVVD
                     EERVYQLIHLLKRAGARDILVMPIERMIP"
     gene            2694..3404
                     /gene="hisA"
                     /locus_tag="PKL26_02540"
     CDS             2694..3404
                     /gene="hisA"
                     /locus_tag="PKL26_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNS82604.1"
                     /translation="MRIFPAVDILNGQCVQLVQGRKESATVFGDPLSCAHRWLDLGAR
                     CLHVVNLDGAFGESSANAGKIRELVRETGVEIQLGGGIRSFSDASSWLETGIERVILG
                     TLATRDPPVLKRLSDEFGSARVMAGVDARGGEITIEGWQKSGGDYLVWAERFENMGVG
                     SLLFTNVDVEGLQSGIDPGPVTRLLRRTRLPVTVAGGVTTAGDIRVLKSLGASGVVLG
                     SALYSGRLHLHEAMEAAE"
     gene            3401..3982
                     /gene="hisB"
                     /locus_tag="PKL26_02545"
     CDS             3401..3982
                     /gene="hisB"
                     /locus_tag="PKL26_02545"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNS82605.1"
                     /translation="MRCVTLTRKTKETSIDLVLDLDSPGRGEISTGIAFFDHMLTAMA
                     THGRFGLSCRAKGDLEVDVHHTIEDTGIVLGQAFRDAIGDGRGITRFSHAIIPMDESL
                     ATVALDCGGRGYLVYRGAFAHPRVGGIEREIFEHFFYSLCIHGGITAHLTMEGKNDHH
                     KCEALFKAFGIAIGAATRLGGDADHVPSTKGVL"
BASE COUNT          938 a         1184 c         1198 g          810 t
ORIGIN      
        1 agaccactgt tgagccgggc cccatcccat cagcgggtcc cgctgttgtc caagtagagg
       61 gacgaacacg aacacccccg cctcagccca ggcaacctga agcgccacgg gcggcatcaa
      121 cacctcccgg ggccgcgaca acccagatac aattctgctt ccactgtggt cacaaactgc
      181 ctccgaatgc aaatttctgc ccatattgcg ggacccgcct tcaccaggcc ggtgcacccc
      241 ttcaatcgcc tccgccattc caaatccaaa ggaccgaaga tgagcctgcc agccttgcgt
      301 caaaggagaa agagaagaga ggtttgtttg gctggctttt ccgaaagaaa tagatcccct
      361 cctttttcgt cttcccgaca actctaaggc ttgttgccgg aaaacgcgta attcattgaa
      421 ggattaaaaa aaggataata cctgacttgt ggcaacctgc caactgttct cccagccgga
      481 gctatattcg cgccttcctc cgttcatgag aaaagcccag gcatcaaaag gaatgattat
      541 gttaccttcc gtcgttttaa taatgattac gcgtccggtg atcgagggat ttcattgaga
      601 cggaatataa agatcgaagc attgcaccag atcccgctgg aacgccagga gatcgaactc
      661 gttgagcgga aatgcatagg gcaccctgac agccttgccg atgggattgc cgagtccatc
      721 agccgggctc tctcaaaggc atacctggag gagtgcggtg caatcctgca tcacaatacc
      781 gaccagggcg agatagtggc cggagagtcc cagccaaaat acgggggagg gaaagtcata
      841 cggcccatct acatgttaat tgacggaagg gccaccaagc agttcgatgg gatcaatata
      901 cccaccgatg caatcgccgt cgaggcagcc cggacttacc tccgcgagac tctcaccaac
      961 ctcaacatgg agcgggatat catcgtcgac tgtcgcctgg ggacaggttc aacagacctc
     1021 agggatgtct tcaagccctg ccagaacact ccgccgcgcg ccaatgacac gtcttttggt
     1081 gtagggcacg cgccattctc cgaaaccgag acgatcgtca aggcagtgtc agattacatt
     1141 gacgggacgt tccgcccccg gtaccctgca gtcgggcagg atatcaagat catggggctt
     1201 cgggacgggg acaccatcac gctcacactg gggtgtgcaa tggttgaccg ctattgttca
     1261 ggcctccccg agtacctcga atacaaggat ctcctcagag agcacatcct caatgtcgca
     1321 cgccagcaca ccggacgaaa agtcatcgcc gctatcaaca ctgccgacga catcgatacc
     1381 gggagcgtct ttttgaccgt cacggggaca tcggccgaaa tgggggacga cggctcggtg
     1441 gggaggggaa accggtgcaa cgggctcatt acccccaacc ggcccatgag catggaggcc
     1501 acgagcggaa agaaccctat caaccacatc ggaaagattt acaatgtcat ctccacgcag
     1561 cttgcccagg agtgcgttgc aaaggttgac gggattgaag agatctatat caggcttctc
     1621 tcccagatcg gcaagccaat cgatcaaccc ctcgttgcaa gtgtgcagat cctgccaggc
     1681 gagggtgtgg atatgaagac catcagcccg gatatcgaag ccatcatcga caatgggctg
     1741 gccgagatca cctgcatcac cgagaagatc atccgtggcg agatcaagac gttctgaagg
     1801 tgaggagagc cccgggccgg ttgccaggcc ccaggttctg cgcctggcaa tcccgaacaa
     1861 gggccggata tctgtcccag tcaacgagct gatggagaag agcggactcc acctcctcga
     1921 tgtcggggaa cgccgcctcg tttcacgcac tcccgacccc cacgtggaga tcctttttgc
     1981 gaggcccgtg gatatccccg agtacgtggc caacggggca gctgacctcg ggattaccgg
     2041 gcacgacatg gtcctcgagc ggaaatccga tgtatccgag ctccttgacc tgaaaatcgg
     2101 gtccgcgtcc ctggtgcttg ccgtccgtga agatgccggg tatacgaatc cgttggatct
     2161 cgagggaacg agggttgcga ccgagtttcc tgtcatctca aaggaatatt tccaggagaa
     2221 gggtatctca gtcaccctgg tgcctgtcgg cggggcatgc gaagccacac cgtatcttgg
     2281 gatagccgag gcgatcgtcg acctctccag ctcggggaac acgctccgtt cgaaccgcct
     2341 ccgggtaatt gataccgttc ttcccacgac caccatgctg atcgcaaacc cccagtccct
     2401 tgcctcgaag ggaggaaaga tcgaggagat tgtccttgct ctcgagagcg tggtccgggc
     2461 cagagggcag tgctacctga tgatgaatgc gaagcgtgcc tgcgtcgatg aggtcacatc
     2521 cgtcctcccg gggctctccg ggccgacggt gatggaggtg gcctcgcggc aggacatggt
     2581 cgctatccat gcagtcgtcg acgaagagcg ggtctaccag ttgatccacc tcctgaagcg
     2641 ggcaggagcg cgggatatcc tggtcatgcc catcgagcgg atgatcccct gacatgcgca
     2701 tatttcccgc cgtcgatatt ctgaatggcc agtgtgtcca gctggtgcag ggaaggaagg
     2761 aatcggccac agtcttcggt gacccgctct cctgtgcgca tcgctggctc gacctcggcg
     2821 ccaggtgcct gcacgtcgtg aaccttgacg gcgcatttgg agaatcctcg gcaaatgcag
     2881 gaaagatcag ggaactcgtc agagagacgg gagtcgagat acagctcggc gggggaatac
     2941 ggtctttctc ggatgcctcg tcgtggctcg agaccggcat cgagcgggtc atccttggga
     3001 ccctggcgac ccgggatccc cctgtcctga aacggctgag cgatgagttc ggcagtgccc
     3061 gcgtcatggc aggtgtcgac gcccgtggtg gggagatcac catagagggg tggcagaagt
     3121 ccgggggaga ctatcttgta tgggcagaac gatttgagaa tatgggtgtg ggatcactcc
     3181 tctttaccaa cgtggacgtg gaggggctgc agtcagggat cgatcccgga cccgttaccc
     3241 ggctgctgcg gcgcacccgc ctccctgtaa cagtcgcggg gggcgtcacc acggccgggg
     3301 acatcagggt cctcaagtcc cttggtgctt ccggcgtagt gctcgggtct gcactttaca
     3361 gcgggaggct tcacctgcat gaagccatgg aggcggcaga atgagatgcg taaccttgac
     3421 aagaaagaca aaagagactt caattgatct ggtcctcgac cttgacagcc cggggagagg
     3481 agagatctcc actggaatcg cgttttttga tcacatgctc accgccatgg ccactcacgg
     3541 acgtttcggc ctctcctgca gggcgaaagg cgacctcgaa gtggacgtcc atcacaccat
     3601 agaggacacc gggattgtcc ttggccaggc attcagggat gcgatagggg acggcagggg
     3661 gatcacgagg ttttcgcatg cgatcatccc catggatgaa tcgcttgcca ctgttgccct
     3721 cgattgcggc ggtcggggat acctcgtcta caggggtgca ttcgctcatc cccgggtggg
     3781 gggaattgag cgagaaatat tcgagcactt cttctacagc ctctgcatcc atggcgggat
     3841 caccgctcac cttaccatgg aggggaagaa cgatcaccac aaatgcgagg cactcttcaa
     3901 agcattcggc attgcgatcg gtgcagccac ccgccttggc ggagatgccg atcatgtacc
     3961 gagcaccaag ggagtcctct gattttaaaa aatggtgcag gagagcggaa aagggcattt
     4021 cagggtccct ccccccatgg gatcccagaa ataacccgct caattcctta aaaaaagcga
     4081 agggaattgt tttcaacagg tgatgaaagc ctggtaagcg cttcttttgc
//
LOCUS       DAOQVJ010000076         4088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_24571_length_4088_cov_4.500372, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000076 DAOQVJ010000000
VERSION     DAOQVJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24571_length_4088_cov_4.500372"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2498
                     /locus_tag="PKL26_02550"
     CDS             <1..2498
                     /locus_tag="PKL26_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNS82606.1"
                     /translation="RARISHNSIVFSLDSDGVVTYASPAVVRILGYLPEEVTGHRFTE
                     YLHPSDIQEVMEGLALLQKERNSKPFSVEWLVKTKVGDERWVRISLRTLWDGERYSGC
                     TGTITDINEQRRAGEELREREALLQGIFRAAPVGIGIVQDRVLSWVNAQVARMTGYSP
                     EELAGRSARILYESDEEFVRVGEEKYPPMWETGVGSVQSRWVRKDGTVFDVLISSSPL
                     NPGDRHSPIVFTVSDITDQKRAEAAARAMERKYRELVDDIPDILYTADLEGHITFMNQ
                     AGLGAFEVTEEEMIGQTFEQNLHPEDIRAGIAIFQGILQTGEPVMNFECRFVARRGEG
                     RVFPVINNVSVMRDEHGRIVGTRGIALDITEKKKAEDELREREERLQMIAKQVPGSLW
                     TTDTNLVFTSSYGAGLGDMGLLPNQVVGMKVEAFHGNHPGALEAVSAHRRALNGESVS
                     YTAYYRDRVYKAHLEPLKGNDEKTIGTLGIAFDITDLQKAEEALTESEHLLRTFINAI
                     PEPACLIDPEGRIILANAAFCSRYSIPDTAVAGMNAYTLVNGEMGGTMQARVGDVFST
                     GKEIRFEIAQKDRVSLANVYPVRDSIGKVTRAAVFSVDITDQKKAQEVLSLLNKKLSL
                     LSRITRHDALNRIAVLASYLELMEEQTDDPLLITYIRKEKRGIEALQALLAFTESYQN
                     VGMKSPSWQDVSAAVRRGAELQDLVHVTIYLDTEGLEIYADPMLEKVFFSLIENAVRY
                     GGSLTRITFSSQEDEGGLLLVCEDDGTGIPAEQKEAVFSLGVGQNTGYGLFLVREVLA
                     ITGFSIRETGEPGKGARFEIRVPHGAFRRSASP"
     gene            complement(2548..2991)
                     /locus_tag="PKL26_02555"
     CDS             complement(2548..2991)
                     /locus_tag="PKL26_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82607.1"
                     /translation="MRLQPIFLVVLVCGMIFITGCTQQEVQVTPTPTPTPEAPPPDIS
                     PLPTDTVPTEYRVFVTVTRNTYSFHPLIKVEFRGGQGMGLLSSLDAEVIREDGSAGTA
                     SLQRPAIGEFMEVQGSTGKDRVIVHAHMMNGARYRIYDDVLEFRT"
     gene            complement(3211..3366)
                     /locus_tag="PKL26_02560"
     CDS             complement(3211..3366)
                     /locus_tag="PKL26_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82608.1"
                     /translation="MCTVGGPVDMDLPPERVKGKNYRCKDCGEKFKGVGRHPMCPSCQ
                     SEDVEEV"
     gene            complement(3526..>4088)
                     /locus_tag="PKL26_02565"
     CDS             complement(3526..>4088)
                     /locus_tag="PKL26_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNS82609.1"
                     /translation="TGDPDTFTQVMVENDRWAYTWNTGRVSGGLAPGSHTVFVSTQPA
                     AANALSGVNYAEIEVILHAAVTTATLEVESSPADAAIYLNGKYSGNSPRVFRDLAPGE
                     YAIRLEKQGYTDETGSVTLAAGDDVKFTRTLSPEGTPDTTVATLPATFPKVGETPPPA
                     PTTTPLTSACIAGTLVLAGLFFRKPD"
BASE COUNT          889 a         1119 c         1225 g          855 t
ORIGIN      
        1 gccgggctcg cataagtcac aactccatcg tcttctcgct cgattcggat ggagttgtga
       61 cttatgcgag cccggctgtg gtccgtatcc tcgggtacct gccggaggag gtgacgggtc
      121 accggttcac tgaatacctg cacccctcag atatccagga agtcatggaa ggacttgccc
      181 tgctgcaaaa ggagcgtaat tcaaagccat tttctgtaga atggctggtg aagacaaagg
      241 tgggcgacga gcgatgggtc aggatatccc ttcgcaccct gtgggatggg gagcgatatt
      301 ctggctgcac cggcactatc acggatatca acgagcagag gagggcgggc gaagaactcc
      361 gtgagcgcga ggcgctcctc cagggcatct tccgggcggc tcccgtgggc atcggcatcg
      421 tgcaggaccg tgtcctctcc tgggtcaatg cacaggttgc caggatgacc ggctactctc
      481 cggaagaact ggcggggaga agcgcccgga tactctacga gagcgacgag gagttcgtga
      541 gggtcggcga ggagaagtac ccccccatgt gggagacagg ggttgggtcc gtccagagcc
      601 ggtgggtcag gaaggacggc actgtctttg acgtcctcat cagttcctcg cccctcaatc
      661 ccggcgaccg gcactcgccc attgtgttca cggtctcgga tatcaccgac cagaaaagag
      721 ccgaggctgc ggcccgcgca atggaacgaa agtatcggga actcgtggac gatattcccg
      781 atatcctcta cactgcggac cttgaagggc atatcacctt catgaaccag gccgggctcg
      841 gggcattcga ggtcacggag gaggagatga ttggccagac gttcgaacag aacctgcacc
      901 ccgaagatat ccgcgcaggg attgcgatat tccagggaat cctccagacc ggggagcctg
      961 tgatgaattt tgagtgccgc ttcgtcgccc ggagggggga gggccgtgtg ttcccggtga
     1021 tcaacaatgt gagcgtgatg agggacgaac acgggcgtat cgtaggcacc cgtggaatcg
     1081 cccttgatat cacggagaag aagaaggctg aggacgagtt gagggagagg gaagagcggc
     1141 tgcaaatgat cgcaaagcag gtccccggat ctctctggac caccgatacc aacctcgtat
     1201 tcacttcatc ctatggtgcg gggcttggcg acatgggcct tctgcccaac caggtggtgg
     1261 gaatgaaagt ggaggcattc catggaaacc accctggtgc cctcgaagcg gtttctgcac
     1321 acaggagagc gctcaatggt gaatcggtga gctataccgc atattaccgg gacagggtct
     1381 ataaggccca tctggagccg ctcaagggca atgatgaaaa gaccatcggg acccttggaa
     1441 tcgcattcga catcaccgat ctccagaagg cagaggaggc actgacagag agtgaacacc
     1501 tgctccgcac gttcatcaac gctatccctg aacctgcatg cctcatcgat cctgaaggcc
     1561 ggattatcct tgcgaatgcg gcgttctgct cccgttattc gataccggat accgcggtag
     1621 cggggatgaa tgcctacacc ctggtcaacg gggagatggg aggaacaatg caggctcgcg
     1681 tgggcgatgt cttctccacg ggaaaggaga tacgtttcga gattgcccaa aaagatcgcg
     1741 tctctcttgc aaatgtctat cctgtgcggg attcgattgg caaggtaacc cgcgcagcgg
     1801 tattttccgt tgatatcacc gaccagaaga aggcccagga agttctctct cttctcaaca
     1861 agaagctctc actcctctcc cgcatcaccc gccacgatgc cctcaacagg attgccgtcc
     1921 tggcaagcta cctcgaactg atggaagagc agacagatga tccccttctc atcacgtaca
     1981 tcaggaagga gaagcggggt atcgaggcgc tgcaggccct ccttgcgttc accgagagct
     2041 accagaacgt gggaatgaaa tccccctcat ggcaggacgt gagtgcggca gtcaggagag
     2101 gtgccgaact gcaggatctc gtccatgtca ccatctacct ggataccgaa gggctcgaga
     2161 tctatgcgga cccgatgctc gagaaggtct tcttctccct gatagagaat gctgtccggt
     2221 acggaggaag cctcacgcgg atcacattct catcgcagga ggacgagggc gggctccttc
     2281 tcgtctgcga ggacgacggg accgggattc ccgccgaaca gaaagaggcc gttttctcgc
     2341 ttggcgtggg ccagaacacg gggtacgggt tgttccttgt ccgcgaggtg ctcgcaatca
     2401 cgggattctc gataagggag accggcgaac cggggaaagg ggcgcggttt gagatacgtg
     2461 ttccgcatgg tgcattccgg cgttccgcgt ccccgtaacg ggtcggcgga tttcaggttc
     2521 aaaaaaaatc tggaaaagaa gttgatttta cgtcctgaac tccaggacat cgtcataaat
     2581 acgataccgg gcgccgttca tcatgtgtgc atggacgatt acccgatcct tgccggtcga
     2641 gccttgcact tccatgaact ctccgatcgc cggtctctga aggctggctg tccctgcaga
     2701 gccgtcttcc cggatgacct ctgcatcaag cgaagagagg aggcccatcc cctgcccccc
     2761 cctgaattca accttgatga gagggtgaaa ggagtaggta ttccttgtca ctgtcacgaa
     2821 tactctgtat tctgtgggca ctgtgtcggt cggtaacggg ctgatatcag ggggtggagc
     2881 ttctggtgtg ggggttggtg tgggagtgac ctgcacctcc tgctgcgtgc agccggtgat
     2941 aaagatcatg ccgcacacga ggacgacgag aaagattggc tgtagtctca tgggaccttt
     3001 tatgtcccca ggcttaaaaa aatgagcgat ctccccttca gcaggtgctt caatatataa
     3061 cccacgctat ggcttcccct gaaaaagaaa aaagatcctg aaaaattctt tgatttgcct
     3121 ctccattcgg gacatcccct cgcagtcagg tatctgcctg gaaatggatg gacaattgat
     3181 tgtggggaga tctattctcc ggatctccgg ttatacctct tccacgtcct ctgactggca
     3241 ggaaggacac atcgggtgcc tgcccactcc cttgaacttc tccccgcagt ctttgcaccg
     3301 ataattcttg cccttcaccc gttccggggg gaggtccatg tcaacggggc cgccaactgt
     3361 gcacacgctc gtttcacctc accagaaggg atggggagga ggagatataa ggttaactct
     3421 tcggggctcg atcgggaaaa atcaccttga atcctcaggg tatgcatgac tgtcagggcc
     3481 ggaggttccc agaacatccc gcgccgggaa aagaagatgc agggatcaat caggcttcct
     3541 gaagaaaagt ccggcaagga ccagcgttcc ggcgatgcag gctgaagtga gaggcgtggt
     3601 ggtcggtgcc gggggcggag tctcccccac tttcgggaat gtggcgggca gtgtcgccac
     3661 cgtggtatca ggtgttcctt ccggcgagag cgtgcgtgtg aacttcacgt cgtctcctgc
     3721 tgccagggtg accgaccccg tctcatctgt atatccctgc ttttccagcc ggatcgcata
     3781 ctcccctggg gcaaggtctc tgaatacgcg agggctgttg cctgagtact tcccgtttaa
     3841 gtatatcgca gcgtctgcag gactcgattc aacttcgagg gtcgcggtcg taaccgcggc
     3901 atggagtata acctcgatct cggcatagtt cacgccggag agtgcatttg cagccgcagg
     3961 ctgtgtcgag acgaacacgg tatggcttcc cggggcaagt ccccctgaga cccgtcccgt
     4021 gttccacgtg taagcccacc ggtcgttctc gaccatgacc tgggtgaagg tgtccggatc
     4081 cccggtga
//
LOCUS       DAOQVJ010000077         4083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_24607_length_4083_cov_4.142502, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000077 DAOQVJ010000000
VERSION     DAOQVJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24607_length_4083_cov_4.142502"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKL26_02570"
     CDS             <1..1003
                     /locus_tag="PKL26_02570"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNS82610.1"
                     /translation="QEPLRKALSQILLYYTLIGFIAVVMGIVMAYVIARFLTTPITEI
                     LEDVSRIAQGDLGHTIRSMPNPEFRRLEESINLMIGKIQQFSREIEREKAEIRIAAEI
                     QRTFLPREIPESERFEVAAMNIPAQEVGGDFYDFIPLNERSLGLVIADVAGKGIPAAL
                     FMALSRSILRAIATADQRVEETIEESNSLIAADAAAGMFVTLFYCTLDKETGEVSYVN
                     AGHNPPLHYSQKERRLRTLLPTGMAMGVMPDLSYSVGHLTLDSGDVLVLYTDGVTEAF
                     NQNDEEFGEGRLMDAAVSSRDMSAREILDTIYGKVTEFTGGAMQSDDITLVVVRCRR"
     gene            complement(1118..1315)
                     /locus_tag="PKL26_02575"
     CDS             complement(1118..1315)
                     /locus_tag="PKL26_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82611.1"
                     /translation="MTKKEDPGYEKVRCCHCEGAGCIYCDKTGYVLVKSPACLCRHCE
                     GGCCIYCGFTGWAGLKGKYDE"
     gene            complement(1458..2996)
                     /gene="pap"
                     /locus_tag="PKL26_02580"
     CDS             complement(1458..2996)
                     /gene="pap"
                     /locus_tag="PKL26_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNS82612.1"
                     /translation="MEQWDGAPGITLPAHASSRELNDDQYHAIHESQRTQAAALQRQA
                     HDLGIPFIILFEGWYGSGISQMVRDLNRTLDPRGFSYHPIAAPGPLAREHHFLWRFWI
                     RTPARGRIAIFDRGWYARTILDRMDPRKESRVSGSYVDEILGFERTLADSGVALVKMF
                     LHISKKEQKQRLRRLKKDPLTMGHLEGDLDRYIRYRKYYSLVEDLLSRTSTGQSPWHV
                     IDAEEKKYTAVTVFDTILRRLEGAIGEAAKSIVHEAKPERATGENQPSIEHSAIGKAD
                     LTLSIDRTEYDSRMRSLGKALAESQIRLYQEKVPLILIFEGWDAAGKGGNIMRVTRSL
                     NPRGYAVMPVGPPDAGERGRHYLWRFFPALPARGHITIFDRSWYGRVLVERIEGLCRR
                     DEWTRAYREIREFEGSLAEDGAIIRKFWLHIDRDEQLSRFHQREADPEKQWKITEEDW
                     RNRARWDEYEVAVDQMIHLTSTPEAPWVVVEANDKYYSRVKTLQAIVDAVDSCLADKS
                     CSLL"
     gene            complement(3004..>4083)
                     /locus_tag="PKL26_02585"
     CDS             complement(3004..>4083)
                     /locus_tag="PKL26_02585"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNS82613.1"
                     /translation="CQGLYTANTMACMTEAMGMSLPGCAAMPAVEAAKLRIARESGER
                     VLDLVNEGICPRDIITRRSLENAIRVDMALGGSSNTVLHLMAIAAEAEVDLSLDSFTR
                     IGEQIPHLCNMMPAGPHSMQALHRSGGIPAVLKAISDHLEDSPTVSGRRIKEIAHDVK
                     VPSRSIIRSTRDPVSPAGGLRILFGSLAPQGSVIKSIAVNREMWTHTGPARVFDGEQE
                     AMKAILAREIHEGDVIVIRYEGPKGAPGMPEMLSPTSAIVGLGYSRVALVTDGRFSGG
                     TRGPCIGHVAPEAAAGGPIGLVRDGDRIAIDLHGRTLDLLVDASELGKRKTGWSPKER
                     SLKGVLARYAAMVGQADRGAVLERR"
BASE COUNT          817 a         1233 c         1142 g          891 t
ORIGIN      
        1 ccaggaacca ctgcgcaagg cactctcaca gatcctcctc tattacaccc tcatcgggtt
       61 cattgccgtc gtaatgggta tcgtcatggc ttacgtcatc gcccggtttc tcaccacacc
      121 catcaccgag atactggagg atgtcagcag gattgcacag ggcgacctcg gccacaccat
      181 ccggagcatg ccgaaccccg aattccggag gctggaggag agcatcaacc tgatgatcgg
      241 caagatacag cagttctccc gggagatcga acgagaaaag gcagagatca ggatcgccgc
      301 cgagatacag aggaccttcc tgcccaggga gatccccgaa tcggagaggt tcgaggttgc
      361 cgcaatgaat atcccggcgc aggaggttgg tggcgacttc tacgacttca tccccctaaa
      421 cgagcgcagc cttgggctgg tcatcgcaga cgttgccggg aaggggattc cagccgcact
      481 cttcatggcc ctctcccgct cgatactccg tgcaatcgcg accgccgacc agcgcgtgga
      541 agagacgatt gaggaatcga acagcctgat tgctgccgat gctgccgcag ggatgttcgt
      601 gacgctcttt tactgcaccc ttgacaagga gacaggcgaa gtcagttacg tgaatgccgg
      661 ccacaacccc ccgctgcatt actcacaaaa ggagagacgg ctgcgcaccc ttctccccac
      721 cgggatggca atgggtgtga tgccggatct gagctactcg gtgggccacc tcacccttga
      781 ttcaggggat gtgcttgtcc tttacaccga cggggtgaca gaggcgttca accagaatga
      841 cgaggagttc ggggaaggga ggctcatgga tgcagcagtc tcgtccaggg acatgagtgc
      901 cagggagata ctcgatacca tttacgggaa ggtgacagag tttaccgggg gcgcgatgca
      961 gtccgacgat atcacgctgg tggtcgtcag gtgcaggaga taatgtctcc gattgggcca
     1021 ggcaacccag gactgccgcc ctgtttcctg cggttgaatg cccagaaaaa aaatattata
     1081 cggctatcag ccagagtcga cctgtgtgag cgcgtggcta ttcgtcatat ttccctttca
     1141 gtcctgccca acccgtgaaa ccgcagtaga tgcagcagcc cccctcgcaa tgcctgcaca
     1201 ggcatgcagg ggatttcacc agcacgtacc cggtcttgtc acagtagatg cagccggccc
     1261 cttcgcagtg gcagcagcgg actttttcgt atcccggatc ttcctttttt gtcattcttc
     1321 tcatggtact gtcggcaggc gcggggttaa gcatttgcaa aagggtcagg aatggcgcgc
     1381 agtctttttg cattcaggaa cacccgggca accatgcagg gaaaaaggag gtaaggggtt
     1441 acgaaccgcc ggaaccctta caagagggaa caggatttat ctgcaaggca gctgtcgact
     1501 gcatcgacaa tggcctggag tgtcttcacc cgggaatagt acttatcatt cgcttccact
     1561 acgacccagg gggcttctgg agtgcttgtg aggtgaatca tctggtccac cgcaacttca
     1621 tactcgtccc atcgtgcgcg gttcctccag tcctcttcgg ttatcttcca ctgtttttca
     1681 gggtctgctt cacgctggtg gaaccgactg agctgctcgt cccggtcgat atgcagccag
     1741 aacttcctga taattgcccc gtcttctgca agagagcctt cgaactccct tatttcacgg
     1801 tatgcccttg tccattcgtc ccttctgcag agaccttcga tacgttctac gagcaccctg
     1861 ccgtaccagc tgcggtcgaa gatggtgata tggccccggg cagggagcgc agggaagaac
     1921 cgccagagat agtggcggcc acgttcgccg gcgtccggag gcccgacggg catcactgca
     1981 tatcccctcg ggttgagcga gcgggtcacc ctcatgatat tccccccctt ccctgctgca
     2041 tcccatcctt cgaagatgag gatgagaggg actttctcct ggtaaagcct tatctggctc
     2101 tccgcgaggg cctttccaag cgatctcatg cgggaatcgt attctgtcct gtctatcgag
     2161 agggtgagat cggccttccc aattgcagaa tgttcaatcg agggttggtt ttcgccggtg
     2221 gctctctctg gttttgcctc atgaacaatg ctctttgcag cctcgccgat tgccccttcc
     2281 agcctccgga gtatcgtgtc aaagacggtc actgcggtat atttcttctc ttccgcatcg
     2341 atcacgtgcc agggcgactg gccggtgctg gtacgggaga ggaggtcttc tacgagggag
     2401 tagtacttgc gatacctgat ataccggtcc aggtcgcctt cgaggtgacc catcgtcagc
     2461 gggtcttttt tgagcctccg cagcctctgc ttctgctctt ttttactgat atgcagaaac
     2521 atcttcacga gcgcgactcc gctgtctgca agggtgcgct cgaagccgag gatctcgtca
     2581 acatatgaac cggatacacg actctctttc ctggggtcca tccggtcgag gatggtccgt
     2641 gcataccagc ccctgtcaaa gatggcaatc cgccctcttg caggagttct tatccagaaa
     2701 cgccagagaa aatggtgctc tcttgcgagt gggccaggtg ctgcgatggg atgatacgag
     2761 aatccccggg ggtcaagggt acggttgagg tcacgcacca tctgtgatat ccccgatccg
     2821 taccacccct caaagaggat gatgaaagga atacccaggt catgtgcctg ccgctggagg
     2881 gccgcggcct gggttcgctg ggattcatgg atggcatggt actggtcatc gttcagctcg
     2941 cgggacgaag cgtgtgcagg gagagtgata ccgggggccc cgtcccactg ctccatcaga
     3001 tcctcaccgt cgctccagca ctgctcccct gtcggcctgt cccaccatcg cagcgtatcg
     3061 tgcgagcacg cctttcaggg acctctcctt cgggctccac cctgtcttcc gctttccaag
     3121 ttcggatgca tcaacgagga gatccagcgt ccgtccatgg aggtcgatcg cgatccggtc
     3181 tccgtccctg acgagcccga tcggcccgcc tgcagccgcc tccggtgcca cgtggccgat
     3241 gcacggccca cgcgtcccgc cagagaacct gccatctgtc acgagtgcaa cgcgcgaata
     3301 accgaggcca acgattgccg acgtggggga gagcatctcg ggcatccctg gtgctccctt
     3361 cggcccctcg tacctgatca cgattacgtc cccctcgtgg atctcccggg caagaatcgc
     3421 cttcatggcc tcctgttcac catcaaatac ccttgccggc ccggtatgcg tccacatttc
     3481 cctgttcacg gcgattgact tgataactga tccctgcggt gcaaggctcc cgaagaggat
     3541 cctcagtccc ccggcaggac tgacaggatc tcgtgtgctc ctgatgatac tccgggaagg
     3601 aaccttcacg tcatgcgcga tctctttgat ccgtctcccc gaaactgtcg gggagtcctc
     3661 aaggtggtca gatatcgcct tcaggaccgc tggaatgccc cccgaccggt ggagtgcctg
     3721 catagaatgc ggcccggcag gcatcatgtt gcagagatga gggatctgct cccctatcct
     3781 tgtgaaagag tcaagcgaaa gatccacctc cgcttctgcc gcgatggcca tcaggtggag
     3841 aaccgtgttc gaagaccctc caagcgccat gtccacgcgg atggcgttct cgaggctccg
     3901 gcgggtgatg atatcccggg ggcaaatccc ctcgttcacg agatcaagga cccgttcgcc
     3961 ggactccctt gcaattctca gtttcgccgc ttccaccgca ggcatggcgg cacagccggg
     4021 aagagacatc cccatcgcct cggtcatgca ggccatggtg tttgcagtat aaagtccctg
     4081 aca
//
LOCUS       DAOQVJ010000078         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_24954_length_4046_cov_3.873215, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000078 DAOQVJ010000000
VERSION     DAOQVJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24954_length_4046_cov_3.873215"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1378)
                     /locus_tag="PKL26_02590"
     CDS             complement(176..1378)
                     /locus_tag="PKL26_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNS82614.1"
                     /translation="MPAQVQALTGILKTDLDKVFLVGGCVRDILSGKAPEDIDLLAVL
                     PPKRLLDLGFTYVDPKTTIPVFTMHRGDLGKIEIALPRGKGSAPGTMSDVHEDLRRRD
                     FTINAMACRLSTGELIDPFNGRRDLKDRTIRHVSEAFAEDPLRVFRAFRFACKGFTIA
                     PETMDLIRTMDPELQHIPPERIYNEMMKAMAEERPERFFIYLVKSGVSRDVFREIHRM
                     LDVVAGPPEYHPEGSTFNHAVNVLKFVAGRTRCRYTRLAAFLHDIGKVETPENELPRH
                     IGHEKRGIEVVQRFLNRLKGDNRCRKVCTEIARHHMKAERFRHMRRGKQMRFVNTLHK
                     MDLIDPLLHVVEADYGKDISPAVAPFREVAGYNTAELGLSPEDFEGKSGEQIQDLILQ
                     RQLERLGI"
     gene            1536..1967
                     /locus_tag="PKL26_02595"
     CDS             1536..1967
                     /locus_tag="PKL26_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNS82615.1"
                     /translation="MTIFTACSVASTAYLRALIDRGEDVNQWDEIQGETPLHCASRLR
                     KPEAVGLLLDAGADINGLNEDEETPLHVACTRGYTAIVRLLLDRGADVNIRDALEETA
                     LDKILRWPIEQPSREEILDLFRQYAPEQVMEAYCAPGLRIG"
     gene            1971..2438
                     /locus_tag="PKL26_02600"
     CDS             1971..2438
                     /locus_tag="PKL26_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNS82616.1"
                     /translation="MAEDSIIPIGIDIITASKEGNPEIVQRLIEAGLDVNDRDTGGYT
                     PLHWACQFRFVEVVRLLLKAGADVNVKNTDRTGYQTPLHLACLFQNAQIVRLLLDAGA
                     DPCARNEGEWTALDYVRKFTLATNPMREEILDLFRQYAPEVVMEAYCSPGPGA"
     gene            2438..3244
                     /locus_tag="PKL26_02605"
     CDS             2438..3244
                     /locus_tag="PKL26_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNS82617.1"
                     /translation="MKKKGTEYQERMAVHLFPAFMYAVKDGDEASIRSLVAAGADVNL
                     GERSRFPLRAACAHNRPAIVRLLLELGADVNSTSLEGTTALHWVCHTGNLPVAHILLD
                     HGADVEARNQQASTPLHYLCTNTGVQEQVKEDRRGLATLLIGKGADVNARDRNSVTPL
                     HLASSNFTATDAGDLVRLLLETGADVNAKDNTGRFPLHWAMGNYLPLTCKILLDAGAD
                     PNAPDGGGITPLDQVIALPDSHPAREKILDLFRQYAPEQVMEVWCRRPQP"
     gene            3283..3966
                     /locus_tag="PKL26_02610"
     CDS             3283..3966
                     /locus_tag="PKL26_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNS82618.1"
                     /translation="MKRNPRKQTLLSAARNNDIETVRLLLEHGADANGEEFSEETPIH
                     EAVKCGNIDLVRLLLDHGADVNARNACDETPLHAAAWSRNLKMVKLLLLEAGADPSNR
                     DHGDLTAFDVSCINQHEKDGDDGGIADFLFQYGQENPVAWESERYYSIHKACELHHVA
                     TVRFLIDRGVDLTARDNRGLTAIERLDTRHGGNQKTREQILDLFREHAPELFMEVYCM
                     GSGTRAVGM"
BASE COUNT          870 a         1149 c         1179 g          848 t
ORIGIN      
        1 ttgaggaata cattctttat cgacgaatgt ctcagccccg aacttgtcgc ttacgccagg
       61 tcccgcaatc ttcgagcccg caaacttcca cacccagccc agttgacaac tacatatcgt
      121 tgctcatatt cgtagttgtc aacaagccat caacctccgt accatgctca tgcagtcaaa
      181 ttcccagcct ctccagctgc ctctgaagta tcaagtcctg gatctgctcg cctgacttgc
      241 cctcgaaatc ctccgggctg agccccagct cggctgtatt gtatccggca acctccctga
      301 acggggccac cgcgggggag atgtccttgc cgtaatccgc ctccacgaca tggaggaggg
      361 gatcaatgag gtccatcttg tgaagcgtgt tgacgaagcg catctgcttc ccccgtctca
      421 tgtgccggaa ccgttccgcc ttcatgtggt gcctggctat ctccgtgcag accttcctgc
      481 acctgttgtc ccccttcagc ctgttgagga acctctgcac cacttctatc ccgcgcttct
      541 cgtggccgat gtgcctcggg agctcgttct ccggcgtctc gacctttccg atgtcgtgca
      601 gaaaggcggc gaggcgcgtg tacctgcagc gggtcctgcc agccacgaac ttgaggacgt
      661 tgacggcgtg gttgaacgtc gagccttccg gatggtactc gggagggccc gccacgacgt
      721 cgagcatccg gtggatctcg cggaacacgt ccctgctcac cccgctcttc accaggtata
      781 tgaagaacct ctccggacgt tcctcggcca tcgccttcat catctcgttg tagatgcgct
      841 cgggagggat gtgctgcagc tccgggtcca tggtacggat gaggtccatg gtctccgggg
      901 cgatcgtgaa tcccttgcag gcgaatctga acgcgcggaa cacccgcagg ggatcctccg
      961 cgaaggcttc cgacacgtgc ctgatggtcc ggtctttcag gtcccggcgg ccgttgaacg
     1021 ggtcgatgag ctccccggtg gagagtctgc acgccattgc gttgatggtg aagtctctcc
     1081 tgcggaggtc ctcatggaca tcggacatgg tgccgggagc ggatcctttt ccccttggca
     1141 aggcgatctc tatcttcccg aggtccccgc ggtgcatcgt gaacacaggt atcgttgttt
     1201 tcgggtccac gtaggtgaac ccgagatcga gaagcctctt cggcggcagg accgccagga
     1261 ggtcgatgtc ttccggggcc ttcccggaga ggatgtccct gacgcaccct cccaccagga
     1321 agaccttgtc caggtccgtc ttcaggattc ccgtgagggc ttgtacctgt gccggcatca
     1381 atgccggtgg tatcgtggac aatgaatctg tcaaatatga cggatcgcgc atggcatctt
     1441 tcctggttac ctgtcttgtg gttactattg tacagtagcg gcatagagat aggcaagaat
     1501 ggttgaacac acctgccaga tgctatttct tcaatatgac catctttact gcatgttcag
     1561 ttgcatcgac cgcgtacctt cgagcactca tagaccgcgg cgaggatgtg aaccagtggg
     1621 atgagatcca aggggaaacg cctctccatt gtgcctctcg tttgagaaaa cctgaggctg
     1681 tcggtttgtt gctcgatgcc ggggcagaca ttaacgggct aaatgaagat gaagaaactc
     1741 ccctccatgt ggcgtgtacc cgggggtata cggccatcgt ccgcctcctc ctcgaccgcg
     1801 gggcggacgt gaatatcagg gatgcacttg aggagacagc tcttgacaag atattgcggt
     1861 ggccgataga acagccttcc cgtgaagaga tcctcgatct cttccgccag tacgccccgg
     1921 agcaggtgat ggaggcgtac tgcgcgccag ggttgaggat aggctagaag atggcagaag
     1981 acagcatcat ccccattggc atagacatta ttacagcctc caaagagggt aacccggaga
     2041 tcgttcaaag gctcatagaa gctggtttgg acgtcaacga ccgtgacaca ggcggttaca
     2101 cccccctcca ttgggcatgc cagtttcgtt ttgtggaagt tgtccggctc ttgctgaaag
     2161 ccggggcgga cgtgaatgtg aagaacactg acagaacggg atatcagact cccctccacc
     2221 tggcttgtct cttccagaac gcgcagatag tccgcctcct cctcgatgcc ggggcggatc
     2281 cgtgtgcgcg aaacgaggga gagtggaccg cattggatta cgtgagaaaa tttacgctgg
     2341 caactaaccc catgcgtgaa gagatcctcg atctcttccg acagtacgcc ccggaggtgg
     2401 tgatggaggc gtactgctca ccgggcccgg gagcataatg aagaagaagg gaactgaata
     2461 ccaggagagg atggcggtgc atcttttccc ggccttcatg tatgccgtga aggatgggga
     2521 tgaagcaagt atcaggtcgc tggtggcggc gggagcggat gtgaacctgg gggagcgttc
     2581 aaggttccca ttgcgcgcgg cctgtgccca taaccgcccc gccatcgtcc gcctgctact
     2641 ggagctcggc gcggacgtga actcgacctc acttgaaggc accaccgcgc ttcattgggt
     2701 ctgtcatacc gggaatctcc cggtcgccca catcctactc gaccacggcg cggacgtgga
     2761 ggcgcgaaac cagcaggcgt cgactcccct gcactacctc tgtacaaaca ccggcgtaca
     2821 ggaacaggtg aaggaagaca gaagggggct cgccacgctt ttgatcggga aaggcgccga
     2881 tgtgaacgcc cgggacagaa atagtgtgac gcctctccat cttgcaagca gcaactttac
     2941 ggcaacagac gcaggtgacc tcgtccgtct gttgttggag accggggcgg atgtgaacgc
     3001 gaaggataat accgggcggt tcccgctaca ttgggccatg gggaattatc tgcctttgac
     3061 atgcaagatt ctcctcgacg ccggagctga cccgaatgcc ccggacggag gtggcataac
     3121 cccgcttgat caagtgatcg ctcttcctga ttcccaccct gccagggaga agatcctcga
     3181 tctcttccgc cagtacgccc cggagcaggt gatggaggtg tggtgccgga gaccacagcc
     3241 atgagggaat ccagggcgaa taggaatagg agggccaatg tgatgaaacg aaatccgcga
     3301 aaacagacat tgctctctgc cgcaagaaat aatgatattg agaccgtccg gctcctcctg
     3361 gagcacggcg cggatgccaa cggcgaagag ttttcagagg agacgccgat ccatgaggcg
     3421 gtcaaatgcg gcaatatcga ccttgtccgc cttctcctcg accacggcgc cgatgtgaac
     3481 gcgaggaatg catgtgatga aacacctttg catgctgcag catggtcgcg caatctcaaa
     3541 atggtcaagc tattgctcct cgaagcgggt gcggatccca gtaacaggga tcacggcgat
     3601 ctcaccgcct ttgacgtgtc gtgcatcaat caacatgaga aggacggaga cgatggcgga
     3661 atagcggact ttcttttcca atatggtcag gagaacccgg tggcatggga atccgagcga
     3721 tattattcta tccacaaagc ctgtgaactg caccatgtcg ccacagtgcg ttttctcatc
     3781 gaccgggggg ttgatctaac ggcacgcgac aaccgaggcc tcacggctat tgagagactg
     3841 gacacaagac atggcggtaa ccagaagacg agggagcaga tcctcgatct cttccgggaa
     3901 catgcaccgg agctcttcat ggaagtatac tgcatgggat ccggtactcg tgcggtgggc
     3961 atgtagggaa ccataataaa agacccaccg gatttcgctt tccccggaca aggtgaagaa
     4021 ccaaagatgc gggggaagag gcgcgg
//
LOCUS       DAOQVJ010000079         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_24967_length_4045_cov_3.646366, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000079 DAOQVJ010000000
VERSION     DAOQVJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24967_length_4045_cov_3.646366"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKL26_02615"
     CDS             complement(<1..330)
                     /locus_tag="PKL26_02615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS82619.1"
                     /translation="MNYSDMYQNGEYLRKNPTWDVEDSPWKAEQILIMLQEHNIHPYT
                     IGEVGCGAGEILVSLQRSMDESCAFSGYEISPQAIEICNPKSNAKLHFFQGDLLQEPD
                     INWDLLLA"
     gene            complement(334..1530)
                     /locus_tag="PKL26_02620"
     CDS             complement(334..1530)
                     /locus_tag="PKL26_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS82620.1"
                     /translation="MKFALVSLGLPPSQSGQSLVLYHLLKDLKPDQYSLITQKNFHLY
                     GSQGNGSSHLPGTYHYINPDYQVIRGILRFASTARSTGVLSALLKVRIHQLRRIIRQE
                     RSEAVIACTGDLFDPPAAYQVSKSLDIPYILYAFDHYSHQWMPPLLHAFARKHEEQLI
                     LGAAEIIVPNEFLRREYRRTYDVEATVLHNPCELSRYQANPEHNRANNEKEITILYTG
                     AIYEAHYDAFQNLIQALHTLDRPEVRLHIYTPQSESRLRANNISGEYVTYHKNQPIFM
                     MPEIQQRADILFLPLAFNSPYPEIVKTASPGKIGELLAAKRPVLVHAPADSFVSWYFN
                     QHSCGLVVSENDPVELAKGIERLVSDQNLQQSLGMAAYARAKEDFDDRVIKERFTNLL
                     GAHCIR"
     gene            complement(1562..2650)
                     /gene="wecB"
                     /locus_tag="PKL26_02625"
     CDS             complement(1562..2650)
                     /gene="wecB"
                     /locus_tag="PKL26_02625"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNS82621.1"
                     /translation="MTILGTRPEIIRLSRIIPKLDRLCEHVLVHTGQNYDPNLSEIFF
                     DELGIRAPDHFLGVQGRSFGDQIGQIIHTSEEIMRSEQPDRLLILGDTNSSLSAIVAK
                     RMGIPVYHMEAGNRCYDDRVPEEVNRRIIDHSSDVLLPYTERSRANLLREGIAGERIY
                     VTGNPINEVLEHYRSSIESSNVLERLNLESGRYFLVTMHRAENVDIESRLRALMAAME
                     SLNRTYGVPLICSLHPRTRDAMRRFGLLERISPGIQFHAPFGLFDFIQLEQNARCVLS
                     DSGTVQEECCIFHVPNVTLRDVTERPETIECGSNVLSGVDHDTIVCAVECVLSRDPDW
                     KVPDEYMAGRVSDTIVKVMVGYSPERKR"
     gene            complement(2656..3714)
                     /locus_tag="PKL26_02630"
     CDS             complement(2656..3714)
                     /locus_tag="PKL26_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNS82622.1"
                     /translation="MLTDKTILVTGGTGSLGKVLIKRLLSGEMGCPKKIIVFSRDEAK
                     QHFMRMEYLNRTTATDEVIYDNFKRMLEFRIGDVRDFHSISRALRGVEVVFNTAALKQ
                     VPSCEYFPFEAVLTNITGPENIIRAIREQNLPIDTVVGISTDKACKPVNVMGMTKAIQ
                     ERIFIQANLDCQNTRFICVRYGNVLASRGSVIPLFHDQILNGGPVTITTTDMTRFLLS
                     LDQAVDTIFEAVRYAHRGETYIPRVPSAKVIDIAAVLIGDRSIEMIVTGIRPGEKIHE
                     ILVSEEEAHRTIPRGSYYVIQPILPEIRSEGESGSTIDKEYGSADNVMTPEDLHRELH
                     RHGLLLEDLETHEGELLR"
     gene            complement(3708..>4045)
                     /locus_tag="PKL26_02635"
     CDS             complement(3708..>4045)
                     /locus_tag="PKL26_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS82623.1"
                     /translation="VSGYTNAIFSGLTTNALSDVIATIITDYPEMRGVWHVAAEPISK
                     YDLLTLVKTVYNLSIEIQPDNSVVIDRSLNGNKLRKNTNIIIPSWQTMIEQMHQDSTP
                     YAAMRAKKC"
BASE COUNT          897 a         1023 c         1078 g         1047 t
ORIGIN      
        1 ggcaaggagg agatcccaat tgatgtcggg ttcctggagg agatcgccct ggaaaaaatg
       61 caattttgca ttcgacttcg ggttgcagat ctcgatcgcc tgaggcgaga tctcgtagcc
      121 tgagaaagcg cagctttcgt ccatcgacct ctgcaggctg accaagatct cccccgctcc
      181 acacccaact tcaccgatag tgtatggatg gatgttatgc tcctgaagca ttatcaggat
      241 ctgttcggct ttccagggtg aatcctcaac gtcccatgtg gggttcttcc gaaggtattc
      301 gccgttctgg tacatgtcgc tgtaattcat ccgtcacctg atgcagtgtg cacctaaaag
      361 gttggtaaat ctttctttga tgactctatc atcgaaatct tcctttgcgc gggcatatgc
      421 cgccatgccc agactctgct gaaggttttg atcgctgacc aacctctcga ttccttttgc
      481 aagctcaacg ggatcgtttt cagagaccac caggccgcag gagtgctgat tgaaatacca
      541 tgagacgaaa gagtccgcag gagcatgcac cagtacgggg cgttttgcgg caaggagttc
      601 accgatcttc ccgggtgatg cggttttcac gatctccgga tacggtgagt tgaacgcgag
      661 tggaaggaat aaaatatccg ctctttgttg aatctcaggc atcatgaaga tcggttggtt
      721 tttatggtac gtaacatact ctccggagat gttatttgct ctcaacctgg attccgattg
      781 cggtgtatag atatgaagac gaacttccgg ccggtccaga gtatgtaaag cctggatcaa
      841 attctggaag gcgtcataat gcgcttcata gattgccccg gtatagagta ttgtgatctc
      901 tttctcgttg ttggctctat tgtgctccgg attcgcttga taccgtgaga gttcacaggg
      961 gttgtgcagg accgtcgcct caacatcgta tgtccggcgg tactcccgac ggagaaactc
     1021 atttgggacg ataatttctg ccgctcccag aatgagctgc tcttcatgct ttcgcgcaaa
     1081 cgcatgcagg agaggaggca tccattgatg ggagtaatgg tcgaatgcat acaggatata
     1141 agggatatcg agtgacttgc ttacctggta tgctgcaggg ggatcaaaga ggtctccggt
     1201 acaggcaatg acggcctcag atcgttcctg acggattatc cttctcaact gatggatccg
     1261 taccttcaag agcgcgctga ggacacctgt actgcgtgct gttgacgcaa acctgagtat
     1321 tccacggatt acctgataat caggattgat gtagtggtac gtgccgggca ggtgagatga
     1381 cccatttccc tgactcccat agagatggaa gttcttctgc gtgatcagac tgtattgatc
     1441 gggtttgaga tccttcaata gatgatacag caccagtgac tgtcctgact gggatggggg
     1501 caatccaaga gagacgagag cgaatttcat gaggaccggc acatcccatg tgagggtgat
     1561 ttcatctctt tcgttccggg ctgtacccca ccatgacctt cactatcgtg tcgctgaccc
     1621 tgcctgccat gtactcatcc gggaccttcc agtccgggtc tcgggagagc acacattcga
     1681 ctgcacacac aattgtgtca tggtcgactc cgctcaggac attactgccg cactcgatcg
     1741 tttccggtcg ttcagtcacg tcacggaggg ttacgttggg aacatggaag atgcaacact
     1801 cttcttgaac ggtgccgcta tcgctcaaga cgcaacgggc gttctgctcg agctggatga
     1861 aatcgaagag gccaaacgga gcgtggaact gtatgccggg agagatccgc tccagtagcc
     1921 cgaaccgtct catggcgtcc cgtgtacgag ggtgaagact acagatgagg ggtactccat
     1981 aggtccggtt gagactttcc atcgctgcca tcaatgcacg aaggcgtgat tcgatatcga
     2041 cattttcggc acggtgcatg gtgacaagga agtatcgacc ggactctaaa ttcaatcgct
     2101 caagaacgtt ggaactctca atagaacttc gataatgctc taacacttcg ttgatcgggt
     2161 ttcctgtcac gtagatacgc tcacctgcaa taccctcacg gagaaggttt gcccggctcc
     2221 gttcggtata cggcaacaga acgtcactgg agtgatcaat gatccgccga ttaacttctt
     2281 caggaacccg atcgtcgtag catcggttcc ccgcttccat atggtagacg ggaattccca
     2341 ttcgtttcgc gacgattgcc gaaaggctgc tgtttgtgtc gccgagaata agtagtcggt
     2401 ccggttgctc ggatctcatg atctcttcgg aagtgtggat tatctggccg atctggtcgc
     2461 cgaatgatct cccctggaca cccaggaagt gatccggcgc cctgatcccg agttcatcaa
     2521 agaagatctc gctcagattt ggatcgtaat tctgcccggt gtgcaccagt acatgctcac
     2581 aaagccgatc cagcttcggg ataatccggc tcagccggat gatctcgggc ctggtaccca
     2641 agatagtcat tatcttcatc tgagtaactc accttcgtgc gtctccagat cctcaaggag
     2701 gagcccatgt cggtgcaact ctctgtgcag atcttctggc gtcatcacat tgtcggcaga
     2761 accgtactcc ttgtcgatag tcgatcccga ttctccctcg ctacgaattt cggggagaat
     2821 cggctggata acatagtaac tgccacgggg tatagtacga tgcgcctctt cttcggagac
     2881 gagaatctcg tggatcttct ccccgggcct gatgccggtg acgatcatct cgatagaacg
     2941 gtccccgata aggacggcag caatatcgat cacctttgcc gaggggacgc gggggatata
     3001 ggtctctccc cggtgtgcgt atcgtaccgc ctcgaagatc gtgtcgactg cctgatcgag
     3061 gctgagcagg aaccgcgtca tgtcggtagt tgtgatcgtg acagggccgc cattgaggat
     3121 ctggtcgtgg aagagcggga tcaccgatcc gcgagatgcg agcacattgc cgtaccgaac
     3181 acagatgaaa cgtgtgttct gacaatcaag gtttgcctga atgaatatgc gctcctggat
     3241 agctttggtc atgcccatga cattgacggg cttgcaggct ttatccgtgg agatgccgac
     3301 aacggtgtca atggggaggt tctgttcccg tattgccctg ataatattct ccggtccggt
     3361 gatgtttgtt aagacggctt caaacgggaa gtattcacag gaaggaacct gctttaatgc
     3421 tgcggtgtta aagacgacct ccacgcctcg aagagccctg cttatgctgt ggaagtccct
     3481 gacatcgccg atccggaact cgagcatgcg cttgaagttg tcgtagatga cctcgtcggt
     3541 ggccgtcgtg cggttcaggt actccattcg catgaaatgc tgtttcgctt catcgcgcga
     3601 aaatacgatg atcttttttg gacaacccat ctcgccggaa agcaaccgct ttatcaggac
     3661 cttgccgagc gatcccgttc ccccggtgac cagtattgtc ttgtcggtta gcattttttc
     3721 gccctcattg ctgcatatgg tgtcgaatct tgatgcatct gttcgatcat tgtttgccag
     3781 gaggggatta taatattcgt attctttcgt agcttgtttc cattcaaact tcgatctatt
     3841 acgactgaat tatccggctg gatctctatg ctaagattat agactgtttt gaccagcgtg
     3901 agcaagtcgt atttgctgat cggctcagct gcgacgtgcc agactccgcg catctccgga
     3961 taatcagtga tgatggtagc gataacatcg gaaagggcat tcgtcgtcag gccactgaag
     4021 attgcgtttg tatagccgct gactg
//
LOCUS       DAOQVJ010000080         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_24997_length_4041_cov_3.761666, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000080 DAOQVJ010000000
VERSION     DAOQVJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24997_length_4041_cov_3.761666"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..645
                     /locus_tag="PKL26_02640"
     CDS             214..645
                     /locus_tag="PKL26_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNS82624.1"
                     /translation="MALTSEQEADICSMMDCYAEMYRTRNAKGLFSLFSPGICGYGSG
                     PDEVVKDAAAMKAQVKRDFAQADSIRVRFDIQRVSGEMPVAWVMADCDFEVGVKGTSL
                     CMTGRMTAVLRNTGSRWLFEMVHFAVPAMEQAPGESYPGSA"
     gene            complement(974..1756)
                     /locus_tag="PKL26_02645"
     CDS             complement(974..1756)
                     /locus_tag="PKL26_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82625.1"
                     /translation="MPATTRTTPAAWLLLFIVAAAMCAVPASAEMVIISGDAHSTAAA
                     WNDMGNGHANQHRWDAAIDAYTRAISLDPEYARAFFNRGKAYAALKRFDDAIADYEQA
                     IAIDPSQGPVVAPYLESALAVRYPTIPSGSLVKGYWQTGGQFLAIDNSQGASDLVVAL
                     APRGSRGALLAVYVAKGYSHRFDQVVPQGPYDVYITFGERWDPVTKSFVKVGGYLKWE
                     LPQYFGGAGTQGYTMTFITWQPPGSWWSYTLTPIDRQQFPVL"
     gene            complement(1841..3037)
                     /locus_tag="PKL26_02650"
     CDS             complement(1841..3037)
                     /locus_tag="PKL26_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNS82626.1"
                     /translation="MHARAFLLTCVMAVLVTLGAAASAAVITVSPDHVNPGDTITVTV
                     QGLADESSLKMSWEVYIEAPGPTFLWSVEGLSFPINLQDASFRLTNQNTVTNKVTLQN
                     EIPNYETRELIHSGNSVDGTWTVYHGNDFINGTWPVIRNEGTVEEGVTHVLSLVEWYG
                     IKRANPEIPEQENGGPEDFMIPFSFSGFEDGRVKFTIQVNGTDVLTDEVTVGSPSSRT
                     GTLYLKSSPTSAQVYIDGIYYGLSPRKVTGVSPGIRTVKMTKEGYVDYVEQLTVSAKG
                     LTLVMATLNPATASIKVSSIPSHAEVYLDSALVGTTPMTITGVGPGTHTVALMKEGYQ
                     DYTRTVTITDGETVRLINIFLLKSGFSQFPGMNYPNWSAGGPGSRIDPGAIIERVNAR
                     FDKVRF"
     gene            complement(3131..>4041)
                     /locus_tag="PKL26_02655"
     CDS             complement(3131..>4041)
                     /locus_tag="PKL26_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNS82627.1"
                     /translation="GFGTSEDLGRIAVGNRLGQVAVYDREGFEEAWEDNLLKRYYATS
                     AAGLSPDGRMLAYSLLEHTPAIYLLNVDTWRTRTIRSVFREHGSPVHTLVLSGNGSSL
                     LAASGEGSTDTVYLFTDSGALKWKETVPDILDMEISSDSALILLGSEDGCIRAYNPAG
                     DLTWTRCMEGAVQSLSLTPEGDLLAAGSRGGEILLLSGSGELRWEHRLVRFPAARIDA
                     LEISTQGNALVAVVNRNEVLFFSLGPDTLDSQNSPPEPVAGPLPSTEPTTLETPVPPS
                     LWGIPRAGSGFSEYGSALRAIFRISQ"
BASE COUNT          747 a         1207 c         1282 g          805 t
ORIGIN      
        1 agcgcgacgt gatcgcttcg gttgatgcga cactctcccc gtcaccgacc tcggcaccga
       61 ccaagtcggg cctcccggcc gcactcgcgg ttctcggcat cggcctcctt accgtggcgt
      121 acatggcaaa gaggcgctga agagaacttc cccctccttt ttcccgtgcg cgaagagaaa
      181 cactttcatc ctatcccgtg caacctgcca tccatggcac tcaccagcga gcaggaagcc
      241 gatatctgca gcatgatgga ctgctacgca gagatgtatc gaaccaggaa cgcaaaaggg
      301 ctcttttcgc tcttctcccc gggcatctgc gggtacggga gcggccctga cgaagtggtg
      361 aaagacgctg ccgcgatgaa ggcgcaggtg aagcgggact ttgcgcaggc tgactcgatc
      421 cgtgtccggt tcgatatcca gcgggtctcc ggagagatgc cggtcgcgtg ggtgatggcc
      481 gactgcgatt tcgaggtggg ggtgaagggc acttccctct gcatgaccgg ccgcatgacc
      541 gcggtgctca ggaacaccgg ctcccgctgg ctcttcgaga tggtgcattt cgcggtccct
      601 gccatggagc aggcaccggg cgagtcgtat cccgggagtg cgtagaatat ccccacatct
      661 tttcccgtaa ctctcctgaa gtggcaggga tggccggtta ttttctttga acgaaatcgg
      721 ccctttcccc tgtgccggct cattcgccct gcctgacatg gacgctctca agcctgcccg
      781 acgtacttgg gaacgggatc cccgcaggat ttatttcccc tcactcttat cgggacatct
      841 ggcggttccc gccctccgca ggcattcatc cggagaaggg acatcgttca tgggagtgga
      901 ccttgggcag acggattctt accggctcct tggctgcagc caggtgaccc cgggaaaaaa
      961 gtggtgccag atcctacagc accgggaact gctggcggtc gataggggtg agggtgtagc
     1021 tccaccagga acccgggggc tgccaggtga tgaaggtcat ggtgtaccct tgagttcctg
     1081 cgcccccgaa gtactgcggg agctcccact tcaggtaccc gcccaccttc acaaatgact
     1141 ttgtcacggg gtcccaccgc tcgccgaagg tgatgtagac atcgtagggc ccctggggaa
     1201 cgacctggtc gaaccggtgc gaatacccct tcgccacgta gacggcaagg agcgcgcccc
     1261 ttgaaccgcg gggggcgaga gccacgacga ggtcgcttgc gccctgcgag ttgtcgatgg
     1321 cgaggaactg gccgccggtc tgccagtatc ccttcacaag cgacccgctc ggaatagtgg
     1381 ggtagcggac cgcaagcgcg ctctcaaggt agggtgccac gaccgggccc tgggacgggt
     1441 cgatcgcaat ggcctgctcg tagtcagcga tggcatcatc gaagcgttta agagccgcat
     1501 aggccttgcc ccggttgaag aaggccctgg catattccgg gtccagggat atcgcccggg
     1561 tgtaggcatc gatcgccgcg tcccagcggt gctggttagc gtgcccgttg cccatgtcgt
     1621 tccatgcggc ggcggtggag tgggcgtccc ccgagatgat caccatctcg gctgatgcgg
     1681 gcactgcaca cattgccgct gcaacgatga acaggagcag ccaggctgcg ggggtggttc
     1741 gtgtggtggc aggcataggg tatctcctgt aaaggaatat acggatggtc tttatatacc
     1801 ctcgtacaca gagaggcagg atgagaaaat tggaggggta tcagaacctt accttatcga
     1861 accgtgcgtt caccctctca atgatggcac cagggtcgat ccgcgatcca ggccctcctg
     1921 ccgaccagtt cgggtagttc atccccggga actgtgagaa cccgctcttc aggaggaaga
     1981 tattgatcaa gcgaaccgtc tctccatcgg tgatggtgac ggtcctggta tagtcctggt
     2041 acccttcctt catcaacgcg acggtgtggg tcccggggcc cacaccggtg attgtcatcg
     2101 gtgtcgtccc cacgagcgcc gaatcgaggt agacctccgc atgcgagggg atggaactca
     2161 ccttgatgga cgccgtcgcc gggttgaggg ttgccatgac cagtgtgagc ccctttgcgg
     2221 agacggtgag ctgctcgaca tagtccacgt acccctcctt cgtcatcttt accgtgcgga
     2281 tgccgggcga aacgccggtg accttccggg gcgaaagacc ataatatatc ccgtcgatgt
     2341 agacctgggc cgaggtgggg cttgacttga ggtagagcgt ccctgtccgt gaggagggtg
     2401 atcccaccgt cacctcgtcg gtgaggacat cggtcccgtt cacctggatg gtgaacttga
     2461 cccggccgtc ctcgaatccc gagaaggaga acgggatcat aaagtcctca gggcctccgt
     2521 tctcctgttc ggggatctcg gggttggccc gcttgatccc gtaccattca acgagcgaga
     2581 ggacgtgggt cactccctcc tcaaccgtgc cctcgttcct gatcaccggc caggtgccgt
     2641 tgatgaaatc atttccatgg tataccgtcc aggtcccatc caccgagttg ccggaatgga
     2701 tcagttcccg ggtctcgtaa ttggggattt cgttctggag cgtgaccttg ttggtcaccg
     2761 tattctggtt ggtgagccgg aacgaggcgt cctgcaggtt gatggggaac gaaagaccct
     2821 ccacgctcca gaggaaggtg gggccgggcg cctcgatgta gacctcccag ctcatcttaa
     2881 gcgaggattc gtcggcgagc ccctggaccg tcacggtgat ggtatccccg gggttcacgt
     2941 ggtcagggga gacagtgatg actgcggccg aggcggcggc cccaagggtc accagcacgg
     3001 ccatcacaca ggtgagaaga aatgcgcggg cgtgcatgca tatcacaggc ttcttataca
     3061 attacctgtt tgtgggggca agataaggtt accggaaccc ctcggcgatt ccgggcgcga
     3121 aggaattctg tcactggctt atcctgaata tcgcccgcag tgcgctccca tactcgctga
     3181 acccggaccc ggcgcgcggt atgccccaga gggaaggagg gacgggggtt tcgagggtgg
     3241 tcggttccgt tgaagggagg ggtccggcaa cgggttcggg gggagagttt tgggaatcga
     3301 gggtgtctgg cccgagggag aagaagagca cctcgtttct gtttaccacc gccacaagcg
     3361 catttccctg ggtggagatc tcaagggcat cgatgcgtgc ggcgggaaac cgcacaagcc
     3421 ggtgttccca ccgcagctcc ccgctgccgg agaggaggag gatctctccc cccctcgacc
     3481 cggctgccag caggtctccc tccggggtga gggagagcga ctgcaccgcc ccctccatgc
     3541 agcgggtcca ggtaaggtct cccgcgggat tgtaggcgcg gatgcagccg tcttcagacc
     3601 cgagcaggat cagtgcgcta tccgaagaga tctccatgtc aaggatgtcc gggacggtct
     3661 ccttccattt cagtgccccg ctgtccgtga agagatacac cgtgtcggtg ctcccctcac
     3721 cgcttgcagc cagcagggag ctgccgttcc ctgagaggac cagggtgtgc acggggctcc
     3781 catgctccct gaagaccgac cttatggtgc gtgtcctcca cgtatccacg ttgagcaggt
     3841 agatggcagg ggtgtgctcg aggagggagt aggcgagcat tctcccgtcg ggggagagcc
     3901 ctgctgcgct ggtggcgtag taacgcttca ggaggttatc ctcccaggcc tcctcgaatc
     3961 cctcgcggtc gtagaccgca acctgaccca ggcggttccc gactgcgatc cggcccaggt
     4021 cttccgaggt cccgaacccg c
//
LOCUS       DAOQVJ010000081         4001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_25353_length_4001_cov_4.079067, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000081 DAOQVJ010000000
VERSION     DAOQVJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25353_length_4001_cov_4.079067"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..843)
                     /locus_tag="PKL26_02660"
     CDS             complement(127..843)
                     /locus_tag="PKL26_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNS82628.1"
                     /translation="MDETIMKRMDYKDASDALKKALGLNYSPVAIRMATSHDDIPDGM
                     KQLEGNFRHCKMVDMARREGKVFYAKVENHECMGGAWALGLRPLTETLKNGHFYYKLG
                     KFESTAACKRTIDRIPHVESGVTVATLYAPLEKTNFSPQVVLIIAPARVMLKLAQATL
                     FSLGGRISSEFSGIQSVCADACAQTYLSGKANYSLGCDGSRKFSGIEDGEMVMGFPAE
                     MLPAMVEAVQIVTAAPGSKK"
     gene            complement(926..1738)
                     /locus_tag="PKL26_02665"
     CDS             complement(926..1738)
                     /locus_tag="PKL26_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNS82629.1"
                     /translation="MKIVMVSRIDDEKALEYTRDLGDALLQDENQVCFEPATARALGV
                     DAAWTWDSQSDLVVVVGGDGTILHSVQKMEKQVPVLGINWGEVGFLADLEPGEARAFI
                     KSLRPGFAIEPRMRISLAREGVVLGQALNEALIVTNRPAKMLRFVVVVDGIPAERFRA
                     DGLLISTPTGSTAYAMSAGGPIVDPRIEGFLLVPLAPYMLSSRPHLISNERSVEVRLE
                     SAKPASLVIDGQKSIELGMEGSIVVTRAARPALFVDAKKNFFRKVDHKLRRL"
     gene            complement(1749..2524)
                     /locus_tag="PKL26_02670"
                     /pseudo
     CDS             complement(1749..2524)
                     /locus_tag="PKL26_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
     gene            2613..2996
                     /locus_tag="PKL26_02675"
     CDS             2613..2996
                     /locus_tag="PKL26_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HNS82630.1"
                     /translation="MKEQTEVGKLKEGRYMVIEDEPCKILGISVSKPGKHGAAKARID
                     AVGLFDGVKRSIVQPVSAKTYVPVVERKSGQVITISGNTVTLMDMKEYHNFEIEVPAD
                     KMDRIEMGKEIQYMEALGKRKLDLD"
     gene            complement(3168..3911)
                     /locus_tag="PKL26_02680"
     CDS             complement(3168..3911)
                     /locus_tag="PKL26_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82631.1"
                     /translation="MEKRDMAILLVVLGVVVVMAVVVKPILTGKSPDLTLPFLPGSSP
                     GPTSPPPGYQEVATPSTPEKPASKAPTPTPTWSGTSQSLGYVSLQSPASTPTFTRLPE
                     ETPVPERLLTYASIESKGGGTTQTISMPFPYWELHYTVDPWKTTFFGETESKAAGIAS
                     FTASEVFPSFSIEVKDAADHSLVRRIEPRGGLDAELWEKEKENDPRPWVEKFYEGTTT
                     RSYYFVVHTHMVYSYRMEVKVPERYLGKY"
BASE COUNT          831 a         1157 c         1151 g          862 t
ORIGIN      
        1 gcgagcgcct cgtggagtat gcgagaggga ggagccgtga taatatcacc atagtagccg
       61 caggccgcag gcgaggcctg gcttttcacg gtgattgcat cctggcctga tcacaagaaa
      121 aactggttat tttttggatc ctggtgcagc cgtcacgatc tggacggctt ccaccattgc
      181 agggagcatc tcggccggga accccatcac catctccccg tcttcaatcc ctgagaactt
      241 gcgtgagccg tcgcacccca gggaatagtt ggctttcccg gagaggtagg tctgggcgca
      301 ggcatctgcg cagactgact ggataccaga aaactccgac gagatccttc cgcccaggga
      361 aaagagagtc gcctgagcca atttcagcat tacccgggcc ggcgcaatga taaggacgac
      421 ctgcggggag aaattggtct tctcaagggg ggcatagagg gttgcgactg tcacacccga
      481 ctccacgtgg gggattcggt cgatcgtcct cttgcaggcc gcggtgctct cgaacttccc
      541 aagcttatag tagaaatggc cattcttcag ggtctcggta aggggccgga gcccgagtgc
      601 ccatgcaccg cccatgcact cgtggttctc gactttcgca tagaagactt ttccttctct
      661 cctggccatg tccaccatct tgcagtggcg gaaattgccc tccagctgtt tcattccatc
      721 ggggatatca tcgtggctgg tcgccatcct gattgcgaca ggggagtaat tgaggcccag
      781 cgcttttttc agggcatcgg acgcatcttt gtaatccatt cgcttcatta tagtctcgtc
      841 catttttgca tcacttccgt caattcctta aaaaagatgc tcgggctcat caaagataaa
      901 actgctccta tgagtataaa atgcgttaca ggcgtcgcaa cttgtgatcc actttcctga
      961 agaagttctt tttggcatcg acgaagagcg cgggtctcgc ggcccttgtg accacgatgc
     1021 tcccttccat cccaagctcg atggacttct gcccgtcgat gacgaggctt gcgggcttcg
     1081 cgctctcgag gcgcacctcg acgctccgtt cattgctgat caggtggggg cgcgaggaga
     1141 gcatgtaggg tgcaaggggg acgagaagga atccctcgat gcgcggatcg acaatgggtc
     1201 ccccggcgct catggcatat gcggtggaac cggtgggtgt gctgatcagc aggccgtccg
     1261 ccctgaaccg ttcggcgggg atgccgtcta ccaccaccac aaaacggagc atcttggccg
     1321 ggcggttggt cacgatcagg gcttcgttca gtgcctgccc gagcaccacc ccttcccttg
     1381 cgagagagat tcgcatccgg ggctcgattg caaaccctgg ccgaagggac ttgataaacg
     1441 ccctcgcctc gcccggttcc aggtcggcga gaaagcctac ctctccccag ttgatcccga
     1501 ggacagggac ctgtttctcc atcttctgga cggaatgaag gatggtcccg tcaccaccta
     1561 ccaccaccac gagatcggac tgggaatccc atgtccacgc cgcatcaacg ccgagtgccc
     1621 gggcggtggc aggctcgaaa cagacctggt tttcgtcctg caggagtgca tctccgaggt
     1681 ccctcgtata ttcgagggcc ttctcgtcgt ctatccgtga caccatcacg atcttcattg
     1741 tgtggtccct acctcaggta ctcgatcacc ttgtggtgca ggacgccgtt tgttgccacc
     1801 atgcacctcc cgatggtcac ctcgtcgggg aagtggatgg gcatcccctc aaggtcgctg
     1861 accttacccc ctgcctcggt gcagatcagc atgccggcag ccgcgtccgt gaccctcaat
     1921 gtgccacgga tatcgataaa gccatctatc cgcccgcacc cgatgtagga gagttcgagt
     1981 gcggaggccc caggagacgc caccggcgta tcttctggcc tatgtgcatc accctgcggg
     2041 ggtcaaattt cctcccgtat atgctcatgg cgctctcgtc gaggtttgtc gtccgggagg
     2101 cggtcaatgg ccgcccgtcc agccaggcgc ccttcccgcg aatggcagtg aatgtctccc
     2161 cgctcgcgag gtcttccacg aacccctgga gcacgcatcc cccctctgca taggcgaggg
     2221 agagggcata gaaggggatg cctgcgactg cattgtaggt cccgtctatc gggtcaagga
     2281 agatagtccc tgcatcgccg gagatctcca cctttccggc ctcttcggag ataagggtcc
     2341 tgcagagggg gtggtcctgc aggaaccgca gtacacagtc ctctgccacc tggtcgatca
     2401 gcttggtagg agtgcgatcc gcacccatgc ggacagtccg gccgccctca tcgctaccca
     2461 ccaggtcacg gattgcgccc cgcacttcac gggcaaggag tgtacatgcg tctaaaaact
     2521 ccatggtgcc tgatccgacc gtttctgtag gagtatttat ctgtagatat aaagatacat
     2581 gttactgcgt tttcacgcgg gcgtgaccca taatgaagga acagacagag gtaggaaagc
     2641 tcaaagaagg gcggtacatg gtaattgagg acgagccctg taagatcctc gggatatcgg
     2701 tctcaaaacc aggcaagcac ggagccgcga aggcccgaat cgacgcggtg ggtctctttg
     2761 acggggtcaa gcgctccatt gtccagccgg tctctgcgaa gacctatgtg cctgtggtgg
     2821 agaggaagag cgggcaggtc attaccatca gcgggaacac cgttaccctg atggacatga
     2881 aggaatacca caacttcgag atcgaagtcc ctgccgataa gatggacagg atcgagatgg
     2941 gaaaagagat ccagtacatg gaagccctgg gaaagaggaa gctcgatctg gattgatacc
     3001 acctctgcat tcccgcgagc ccttcttttc aaccattttt cccgctgaaa caccaaaaga
     3061 atcctcgaac ggtgactgaa cgggtcctgc ctgtccggtt ctcgaccccg caaaaactaa
     3121 aagagcggcc tccatgagga atacccgcag acttttcacg aggtttttta gtatttcccg
     3181 agataccgtt ccgggacttt aacctccatc ctgtacgaat ataccatgtg cgtgtggacc
     3241 acgaagtagt aggaccttgt ggtggtcccc tcgtagaact tctccaccca cggtcgcgga
     3301 tcgttctcct tctccttctc ccagagctcg gcatcgaggc cgccacgagg ttcaatcctc
     3361 cgtacaaggg agtgatcggc cgcgtccttc acttctatcg agaaagacgg gaacacttca
     3421 gaagcagtaa aactcgcaat ccctgcagct tttgattcag tctctccaaa aaaagtcgtc
     3481 ttccaggggt ctacggtgta atgcagctcc cagtagggga acggcatgga gatcgtctgg
     3541 gtcgtcccac ctcccttgga ctcgatcgag gcgtacgtga gcagtctctc tggcaccggg
     3601 gtctcttcgg ggaggcgggt aaaagtgggg gtggaggcag ggctctgcag cgagacatag
     3661 ccgaggctct gcgaagtgcc gctccaggtc ggggtcgggg taggtgcctt cgatgcgggc
     3721 ttttccggtg tggagggtgt cgcaacctcc tggtatcccg gtggaggtga cgttggcccg
     3781 ggagacgatc caggcaggaa agggagggtg agatccgggg acttgccggt gaggattggc
     3841 ttgactacga ctgccatgac caccaccact ccaaggacga cgaggagtat ggccatgtcc
     3901 ctcttctcca tgaaaggatc ttcgactgtt tgatattaag aggttacccg taagggaacc
     3961 aagcctacct ggtgcagagc gcagcggtct cgccgtactg g
//
LOCUS       DAOQVJ010000082         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_25436_length_3991_cov_4.648120, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000082 DAOQVJ010000000
VERSION     DAOQVJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25436_length_3991_cov_4.648120"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKL26_02685"
     CDS             complement(<1..655)
                     /locus_tag="PKL26_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS82632.1"
                     /translation="MEKEPLLMLPGPVPMPERVRAAMMRQAINHRGKEFSAVYADCVR
                     ILKTAFGTGNDLFVISGSGTAAMEAAVGNFGRGRKIACLVNGKFGERLYKISQRYGAA
                     QEIGSEWGTPLDLGKLEEALSAGAEMVTLVHNETSAGIRNPAEKIGRMARTHDALLVM
                     DGITSIGGDEVKADAWGADVVVVGSQKCLAAPAGLSAVSVSSRAWGRMTEPRPFFLDL
                     "
     gene            714..1103
                     /gene="purE"
                     /locus_tag="PKL26_02690"
     CDS             714..1103
                     /gene="purE"
                     /locus_tag="PKL26_02690"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNS82633.1"
                     /translation="MVDVAILSGSASDAAIVEKAAAVLQAHGLTYDARVISAHREPDL
                     LDEYIRSSDCRVYIAIAGLSAALPGVIASRTKKPVIGVPVSGKVMGGLDAVLSIVQMP
                     KGVPVACVGVDNGENAALLAIRILDLH"
     gene            1201..2580
                     /locus_tag="PKL26_02695"
     CDS             1201..2580
                     /locus_tag="PKL26_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS82634.1"
                     /translation="MTGRREKRTLLFVSSLASFLVPYTVSSLNIALPAISGAFGLDAV
                     TLGWVTSAYMLTAAVCIVPFGKLADIYGRKRVFLVGNMLFVAGSVFAAVSWAGYALVA
                     ARIVQGLGGAMVFSTSIAIITAVFSPGERGRAIGIVTATVYAGLSLGPFLGGILTQHA
                     GWPSIFLLNVPLGVAVIALTLTRIPGEWADLGQREFDLAGSVLYGLMLIGCIYGLTLL
                     PSTSGLIWMSAGLVLLLAFWWWEHRSPHPVLDPALFSKNHVFLYSNLAALINYAMVFA
                     VGFLLSLYLQFNRGLDPQTAGIFLVAQPVVQTLVSPAAGHLSDSIDPRVLATAGMAFS
                     TAGLGILAFLSTDTPLVVILGGLVILGIGYGLFSSPNTNAIMSSVGVHHLGIASGMVS
                     TMRAIGQMVSLAIAMVVFSVVIGTVQISPAVYPELQQSVNLSFSVFFVLGLIGIWASY
                     TRGNTHIKK"
     gene            2703..3047
                     /locus_tag="PKL26_02700"
     CDS             2703..3047
                     /locus_tag="PKL26_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNS82635.1"
                     /translation="MNKIETVCGYSCSECSYLWKECTGCGNTRGKPFWTQFAGVEICP
                     IYDCCVNDRMLAHCGKCPELICERFTRFKDPGLTEEETRASLRDMEQELRQRSRRESG
                     HSTHPPKRQGES"
     gene            complement(3078..>3991)
                     /gene="thiC"
                     /locus_tag="PKL26_02705"
     CDS             complement(3078..>3991)
                     /gene="thiC"
                     /locus_tag="PKL26_02705"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNS82636.1"
                     /translation="YEAVRRAGSAADVDADLLFSVIRDHCRDGVDFLTLHCGVNREAL
                     SSLHSDPRLMPVVSRGGAFHVAMMVQRGEENPLYAEFDYLLEILAEHGVVASLGDGMR
                     PGCLQDADRHAKTVEYLTLGRLATRALDAGVQRMIEGPGHIPLGQVEYNVLVMKELSN
                     DAPLYLLGPLVTDLAPGYDHLVSAIGGAVAAMHGADFLCMVSPSEHLALPDENDIIEG
                     ARTAKIAAHVGDISRTREGYKHPREIRMAEARRNLDWESQFSLALFGNHAREIHSRDG
                     DLDTCSMCGDLCAVKMVREVFDGEKEK"
BASE COUNT          754 a         1271 c         1100 g          866 t
ORIGIN      
        1 cgagatccag gaagaacggg cgcggttcgg tcatacgccc ccacgccctg ctgctgacag
       61 acaccgctga gagcccggcg ggtgcggcaa ggcacttctg tgatcccacg acaacgacgt
      121 ccgcacccca tgcatcggcc ttgacctcgt cccccccgat cgaggtgatg ccgtccatca
      181 ccaggagagc atcgtgcgtg cgggccatcc ttcctatctt ctctgccggg ttcctgatcc
      241 ccgccgaggt ttcgttgtgc accagggtga ccatctctgc cccggccgag agcgcctcct
      301 cgagtttccc gaggtcgagc ggagtccccc attcggaccc gatctcctgg gcagccccgt
      361 aacgctgcga tatcttatag agccgttccc cgaacttacc gttcacgagg caggcaatct
      421 tcctcccccg gccgaagttc cccaccgcag cttccatggc cgcagttccg gacccgctga
      481 tcacgaagag gtcgtttccg gtcccaaacg ccgtcttcag gattcgcacg cagtccgcgt
      541 ataccgccga aaactccttc ccgcggtggt tgatcgcctg tcgcatcatc gccgcccgca
      601 cccgttctgg catgggtacc gggcctggca gcatcagaag aggttccttt tccattctca
      661 ccagcccata gtatttttat aacccgggca tataagtgtg gatggcgaat ccaatggtcg
      721 acgtagcgat tctcagcgga tcggcctcag atgcggccat tgtggagaag gccgcggcag
      781 tcctccaggc tcacggcctt acctatgatg ccagggtcat ctccgcccac agggagcctg
      841 atctccttga cgaatatata cgatcgagcg actgcagggt ctatatcgct atcgcagggc
      901 tctctgctgc tcttcccggg gtcatcgcct caaggacaaa gaagcctgtc atcggggtcc
      961 cggtgagtgg aaaagtgatg gggggactcg atgcggttct ctccatcgtc cagatgccaa
     1021 aaggcgtccc ggtggcttgc gtcggggtgg ataacgggga gaacgccgcc ctgctcgcaa
     1081 tccgcatcct ggatctccat tgaaagtgct ctttttcctg caggcacgca tacccgcaaa
     1141 ggagtgggtc cagccaatca ggcactaacc tcatctcctc atgcagcgat ctttcctctc
     1201 atgacaggga gaagggagaa gaggaccctc ctgttcgtgt cctctcttgc atccttcctt
     1261 gtcccttaca cggtctcttc gctgaacatt gcgctgcccg cgatatcggg tgcgttcgga
     1321 ctggatgcag tcacgctggg ctgggtcacc tccgcctaca tgctcaccgc agccgtctgc
     1381 attgtcccgt tcggcaaact cgccgatata tacgggagga aacgggtctt ccttgtggga
     1441 aacatgcttt tcgtggcggg gtctgtcttc gcagcggttt cctgggcagg atatgcgctc
     1501 gttgccgccc gcatcgtcca gggcctcggc ggggccatgg tcttctccac ctcgattgcc
     1561 atcatcacgg ctgtcttctc tcccggggag cgcgggcggg caataggaat cgtgaccgca
     1621 acggtgtacg cggggctctc gctcggaccg ttcctcggcg ggatcctgac gcagcatgcc
     1681 gggtggccga gcatcttcct cctgaacgtt ccgctcggcg ttgcagttat cgcactgact
     1741 ctcacgcgga tcccgggaga atgggctgac ctggggcaga gggaattcga cctcgctggt
     1801 tcggtcctgt acgggctgat gctgatcggg tgtatttacg ggctcacgct ccttccttcc
     1861 acttcagggc taatctggat gagtgcaggc ctggtgcttc tcctcgcatt ctggtggtgg
     1921 gaacaccggt caccccaccc cgttctcgac cccgcgctct tctcgaagaa ccatgtcttc
     1981 ctgtattcaa acctcgcagc cctgatcaac tacgcaatgg tctttgccgt gggtttcctc
     2041 ctctcgctct atctccagtt caacagggga ctcgatcccc agacggccgg tattttcctg
     2101 gtggcgcagc cagtagtcca gaccctggta tcccctgccg ccggtcacct ctcggactca
     2161 atcgaccccc gggtccttgc aactgcaggg atggctttct ccacagccgg gctggggatt
     2221 ctcgcattcc tgtccactga cacccccctc gtcgtaatcc ttggaggcct cgtcatcctc
     2281 ggcatcgggt acggcctctt ctcctcgccg aacaccaacg ccatcatgag ttccgtcggg
     2341 gttcatcacc tgggcatcgc atcagggatg gtatcgacca tgcgggccat tggccagatg
     2401 gtcagccttg ccatcgccat ggttgtcttc tccgtcgtga tcggaacagt ccagatctcc
     2461 cctgccgtct accccgaact ccagcagagc gtgaacctct cgttctcggt cttctttgtc
     2521 cttggcctca tcgggatctg ggcatcctac acccggggaa acacgcacat taagaaatga
     2581 ttcgaacctg agaaaacgtg tattcgcgcg caggggatgc cggataccca tgttcacacc
     2641 ctcactttca cactcctctt acctcccttt tttataccaa agattcaccc cttttcatgg
     2701 taatgaacaa gatagagacc gtatgtggtt attcatgcag tgaatgcagt tatctatgga
     2761 aagaatgcac cggttgcggg aatacccggg gaaaaccatt ctggacccag tttgccggcg
     2821 ttgagatatg tccaatatac gactgctgtg tgaatgatcg aatgctggca cactgtggaa
     2881 aatgcccgga gttgatatgt gaacgtttca cccggttcaa ggatcctggc ctgaccgagg
     2941 aagaaacacg ggcatcattg cgcgatatgg aacaggagct ccgccaaagg agcaggagag
     3001 aatccgggca cagtactcat cctccaaaaa gacaggggga gagttaagag gagatttgcc
     3061 ctgaagagat ccgaattcta tttttctttc tccccatcga agacctcccg caccatcttc
     3121 acggcacaga gatccccgca catggagcag gtatccaggt cgccgtccct tgagtggatc
     3181 tccctcgcat ggttaccaaa gagcgccagg gagaactgtg attcccagtc gagattccgg
     3241 cgggcttccg ccatcctgat ttcacgcgga tgcttgtagc cctcccgcgt cctggagatg
     3301 tccccgacat gtgcggcaat ctttgccgtc cgcgcccctt cgatgatgtc attttcatca
     3361 gggagcgcaa ggtgctcgct tggggagacc atgcaaagga aatcggcgcc gtgcatcgcc
     3421 gcgaccgcgc cgccgatcgc agagaccagg tggtcatatc cgggcgcgag atcggtgacc
     3481 agggggccga ggaggtagag cggggcgtcg ttcgagagct ccttcatcac caggacatta
     3541 tactccacct gcccgagggg aatgtgcccg ggcccctcta tcatacgctg gactcctgca
     3601 tcaagagccc gggtggcaag ccgcccaagg gtcaggtatt cgacggtctt tgcatgtcgg
     3661 tctgcgtcct gcaggcaccc cgggcgcatc ccgtcgccaa ggctcgccac caccccatgc
     3721 tctgcaagta tctcgagtag gtagtcgaat tctgcataga ggggattctc ctccccccgc
     3781 tggaccatca tggccacgtg gaaggcacca ccccgcgaga ccaccggcat gagccgagga
     3841 tctgagtgaa gggaagagag cgcctcccgg ttcaccccgc agtggagggt gaggaagtcc
     3901 acgccgtcac ggcaatgatc ccgtatcacc gagaagagaa ggtccgcatc gacatcagct
     3961 gcgctccccg ccctccggac cgcctcgtac a
//
LOCUS       DAOQVJ010000083         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_25529_length_3981_cov_4.150535, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000083 DAOQVJ010000000
VERSION     DAOQVJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25529_length_3981_cov_4.150535"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="PKL26_02710"
     CDS             <1..2284
                     /locus_tag="PKL26_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HNS82637.1"
                     /translation="TVAKVWRSLVEDWNQNKIRIDNFTRMNAGVSIGDTVRISRVQDE
                     IEAKRVVLAPPEDLPRNVPINITPHIVNALIDFPVVKNDLVPLSSGLPFLQTQFVPFK
                     VVEIEPEQAVIITKNTHIEFSEKPAPGVEGVKRISYEDIGGLKDELQRVREMIELPMR
                     HPELFQKLGIEPPKGVLLFGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESE
                     QKLREIFDEAEQNAPSIIFIDELDSITPRREEVTGEVERRVVAQLLTMMDGLEERGQV
                     VVIGATNRVDAIDPALRRPGRFDREIEIGVPTEPDRIEILKIHTRGMPLSEEVNLEQL
                     AQQTHGFVGADLAALAREAGIRALRRYLPEIDLDEEEIPEEVLDTMEVTAGDFRDALR
                     DVSPSAMREVMLEVSHVTWQDVGGLSEAKQEVREAVELPLTDRERFEELGIRPPRGVL
                     LYGPPGTGKTLIAKAVANESGANFIAIRGPQLLSKWVGESERAVREIFKKARQVAPAI
                     IFFDELDALAPTRGGEVGTHVMESVLNQILTEIDGLEELKDVVVLGATNQPMLVDPAL
                     LRAGRFDRLVYIGEPGMEDRAHILTIHLRGMPVEGSAMEALLDSLEGFDEGAIDELGE
                     AIGREKTVSPGEFARIAKTIKVPGKSTLSRSQRRRYLSELMSSRSLKVPDEALEELVR
                     GIAARTEGYVGSDLEALCREAGVFAMREGSRQVAKRHFDESLAKVHPTMNPRLTEYYE
                     RIRQSFKGGLPKEVQPPEYQ"
     gene            2348..3889
                     /locus_tag="PKL26_02715"
     CDS             2348..3889
                     /locus_tag="PKL26_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNS82638.1"
                     /translation="MQNLSAFLDNHALDPSACAIAWPATGERCTYRDLLSRVQRLASA
                     LGGRGISKGDRVCIYLDSCPEYLVSYFAIWRTGAVAVPANIVLKEGELRYLLQDSGAR
                     GIICSSKGYPVARTAVSSCPSLNLVVCTGDAPEGTVPWDLVMQEEPSTGSAAPCGFDD
                     PCQLQYTSGTTGRPKGAILTQGNWMAALEAEREVLGLRPGDRYLGIYPMAHVGVSWGI
                     SVLRAKGTYVIMERFEEEEYLRLAREHRITVLSAMPPVIHGLCDAPKEAEEALGTVRV
                     IISGGGQLLPAVWEAFDRRYRIPIANSYGLSETIVVGTGTATVPGMPELTREYRSVGI
                     PIGYSEVRIVDSSDPSVVLGPGETGEIALRGPSVAIGYWNMPRETAETFLPGGWFLTG
                     DMGHLDEGGALSITDRKKDMIVMSGWKVYPTEVENILLQHPDIADAAVFGTPDERKGE
                     IPVAAVVAREGCRLDGEDVSAFCRGRMAGYKVPRRVIFVDALPRVHGWKLLRRTLREE
                     FSNTP"
BASE COUNT          798 a         1156 c         1343 g          684 t
ORIGIN      
        1 gaccgtggcc aaggtgtggc ggtctctcgt ggaggactgg aaccagaaca agataaggat
       61 cgacaacttc accaggatga acgccggggt cagcatcggc gatactgtca ggatctcccg
      121 cgtacaggac gaaatcgagg caaaacgggt ggtgcttgcg ccccccgagg acctcccgcg
      181 gaacgtcccc atcaacatca cccctcacat cgttaatgct ctcatcgact tcccggtggt
      241 gaagaacgac cttgtgcccc tctcgagcgg cctgccgttc ctccagacgc agttcgtgcc
      301 gttcaaggtg gtggagatcg agccagagca ggcggtgatt atcaccaaga atacccacat
      361 cgagttctca gagaagccgg ctcccggagt tgagggggtc aagcgcatct cctacgagga
      421 catcggggga ctgaaagacg agctgcagcg tgtgcgggag atgattgaac tgccaatgcg
      481 gcaccccgag ctcttccaga aactcgggat tgagccgccg aagggagtcc tcctcttcgg
      541 gccccccggt accggtaaga ccctgattgc aaaggcggtt gcaagcgaga gcggggcgca
      601 tttcatctcg atcgcaggcc cagaggttat ttccaagtac tacggtgaga gcgagcagaa
      661 gctccgcgag atattcgacg aggccgagca gaacgcgccg tcgatcatct tcatcgacga
      721 acttgactcc atcaccccca ggagggagga ggtgaccggt gaggtggagc ggcgcgtggt
      781 cgcccagctc cttacgatga tggacggcct cgaggaacgc ggccaggtgg tggtgatagg
      841 ggccacgaac cgcgtggacg cgatcgaccc ggccctgcgc aggcccgggc ggttcgaccg
      901 cgagatcgag atcggggttc ccaccgagcc cgaccgcatc gagatcctga agatccatac
      961 caggggaatg ccgctttcgg aggaggtgaa cctcgagcag ctggcccagc agacccacgg
     1021 gttcgtcggg gcggaccttg cagccctcgc gagggaggcg gggatccggg cgctgcgccg
     1081 gtacctcccc gagatcgacc tcgacgagga ggagatcccc gaagaggtgc tggacacgat
     1141 ggaggtcacc gccggggact tccgcgatgc gctgcgggac gtcagcccga gcgccatgag
     1201 ggaggtgatg ctcgaggtct cgcatgtgac gtggcaggac gtgggcggcc tctcggaggc
     1261 caagcaggag gtccgcgagg cagtcgaact ccctctcacc gacagggaac gattcgagga
     1321 actgggcatc cgcccgccaa gaggcgtgct cctttatggc cccccgggta caggaaagac
     1381 cctgattgca aaggcggttg cgaacgagag cggggcgaac ttcattgcaa tccgcggccc
     1441 ccagctcctc tccaagtggg tgggggagag cgagcgtgcg gtgcgggaga tattcaagaa
     1501 ggcccgccag gtggcgcccg ccatcatctt cttcgacgag ctcgatgccc tggcccccac
     1561 acggggcggc gaggtgggaa cccacgtaat ggagagcgtg ctcaaccaga tcctgacgga
     1621 gatcgacggg ctcgaggagt tgaaagacgt cgtggtcctc ggtgccacca accagccgat
     1681 gcttgtcgac ccggcgctcc tgcgggcagg ccggttcgac cgcctggtct acatcgggga
     1741 gccgggaatg gaggaccggg cgcatatcct gaccatccac cttcgcggga tgccggtcga
     1801 gggatctgcc atggaggcac ttcttgactc tcttgaaggc tttgacgagg gggcaatcga
     1861 tgagctgggc gaggcgatcg gccgggagaa gaccgtatct cccggggagt ttgccaggat
     1921 cgcgaagacc atcaaggtgc cggggaagag caccctctcg cggtcccagc ggcgccgata
     1981 cctgtccgaa ctgatgagct cccgcagtct caaagtaccg gacgaggcgc tcgaggaact
     2041 ggtgcggggc atcgcagccc gcacagaggg atacgtgggc tctgacctgg aggcactctg
     2101 ccgggaagcc ggtgtctttg cgatgaggga agggtcccgg caggttgcga agcggcactt
     2161 cgacgagagc cttgcgaagg ttcatcccac gatgaatccc cgcttaacag agtattacga
     2221 gcggatacgc cagtccttca agggcgggct cccgaaagag gtccagcccc cggagtacca
     2281 gtagcggctg gagagaaaag agggcgttta atatcgatac ctgacccatt tttctctcag
     2341 gtcttccatg cagaacctat cggccttcct cgacaaccat gccctcgacc cctcggcgtg
     2401 cgcaattgcg tggccggcga ccggcgagag gtgcacctac cgggaccttc tctcgcgggt
     2461 gcagcgactc gcgtcggcgc ttggcgggag gggaatatcg aaaggcgacc gggtctgcat
     2521 ctatctcgac tcctgccccg agtaccttgt cagctacttt gccatctggc ggacaggtgc
     2581 cgtggcggtc ccggcaaata tcgtcctgaa agagggtgaa ctgcgctatc tcctccagga
     2641 ttcaggcgcc cgcgggatca tctgcagcag taaagggtat cctgtggcca ggaccgcggt
     2701 atcctcctgc ccctctctga atctggtggt ctgcacgggg gatgcaccgg aaggcacggt
     2761 cccgtgggac ctggtaatgc aggaggagcc atcgacagga agtgcagccc cctgcgggtt
     2821 tgacgatccc tgccagcttc agtatacgtc tggcactacg gggaggccga agggcgcgat
     2881 cctcacccag gggaactgga tggctgcgct tgaggcagag agggaggtac tcgggcttcg
     2941 ccccggcgac aggtacctcg ggatctaccc catggctcac gtgggggtat cgtggggaat
     3001 ctcggtcctg cgggcaaagg gaacgtacgt gatcatggag cggtttgaag aagaggagta
     3061 cctgcggctc gcaagagagc accgcatcac tgtcctctcc gcgatgcccc ccgttatcca
     3121 cgggctctgc gatgccccca aggaggcaga ggaggcgctt gggaccgtgc gggtgatcat
     3181 cagcggggga ggccagctcc tgcccgccgt gtgggaggca tttgacagga gataccggat
     3241 tccgatcgcc aactcatacg ggctctccga gacgattgtc gtagggaccg ggactgcgac
     3301 tgtccctggt atgccggagc tgacccgcga gtaccggagc gtggggattc ccatcgggta
     3361 cagcgaggtc cgtatcgttg acagctcgga tccatcggtg gtgcttggac cgggcgagac
     3421 gggcgagatc gctctccgtg ggccatcggt ggcaatagga tactggaata tgccgagaga
     3481 gaccgccgag acgttccttc ccggcgggtg gttcctcacg ggggacatgg ggcaccttga
     3541 cgagggcggg gcactctcca tcacggacag gaagaaggac atgatcgtca tgtcggggtg
     3601 gaaggtgtac ccgaccgagg tggagaacat cctcctccag cacccggata tcgcggatgc
     3661 ggcggttttt ggcacacctg acgagaggaa aggcgagatc cctgtcgccg cggtggttgc
     3721 gagggaaggc tgccggcttg acggggagga tgtgtcggcg ttctgccggg gtcgcatggc
     3781 gggatacaag gtgccgagga gagtcatctt tgtcgacgca ctcccccggg tccatggatg
     3841 gaaactcctc cgccgcacgt tacgcgaaga gttctcaaat acgccctgaa tttgtgcaag
     3901 gctataagtg aaaggacaga aatagtacgc tttggagata tcccctttgg ccaaggacaa
     3961 caggcgatcc actggcataa c
//
LOCUS       DAOQVJ010000084         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_25949_length_3935_cov_4.492784, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000084 DAOQVJ010000000
VERSION     DAOQVJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25949_length_3935_cov_4.492784"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..936
                     /locus_tag="PKL26_02720"
     CDS             79..936
                     /locus_tag="PKL26_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNS82639.1"
                     /translation="MKFDARCFDCLLSRVSLECRLCGAEEHLSAEIRDECARLLDALR
                     TQPLSHPQIASAMHRHVYARLGTDDPFRGLKEESTRQALEVCRMVRPRIRSFRDHVLA
                     SVIGNTFDYGVKSHTVKDDFSQYFEEEFQSGLAIDDCDRILPLTSRVVYFTDNCGEIV
                     FDRLLLEFLHRNGARVTLAVREEPILNDATQNEALALHMDRFADVITHTGCGCELGVR
                     LDCMPDPLMQAMEDCTLVISKGMANYESLSEFRDLPPIAYLMAVKCEPIADEVGVPRG
                     SKVAMLRFQ"
     assembly_gap    1123..1222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1627..2304)
                     /locus_tag="PKL26_02725"
     CDS             complement(1627..2304)
                     /locus_tag="PKL26_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HNS82640.1"
                     /translation="MPQEIAVFLGPSCERAVAETILPARFFHPPAARGDLTDAAEEGA
                     RIITLIDGVFFQESAVGHREILAALKSGIRVIGASSMGALRAAELDTLGMEGVGLIYR
                     LYRDGVLISDDEVALVYDPEDYTPLSEPLINIRCTIRKARDEGILTNDDAAALLSAAR
                     SLYFPERTYPHICEQAESAVFAPVRKSFLEFTRLHAVDQKREDALEALRRTKELLGKQ
                     LGEQLGL"
     gene            complement(2264..3478)
                     /locus_tag="PKL26_02730"
     CDS             complement(2264..3478)
                     /locus_tag="PKL26_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HNS82641.1"
                     /translation="MILSPCKKAYKNETQRTTSPEETLARVEKALPAAGITRVADITS
                     LDRVGIPVFSSIRPTAGRGAISVYNGKGATPTEARVSAIMEGIERYSAEVPEGAELRI
                     DRYEEVRRDATVLDPLLLVLPRNADPMMPIPWVEGYDLLQEEDVMVPASAVFHPLPAP
                     YPQLFRTSTNGLASGNTLEEAVFHALMEVIERDAWSLAEASRNTGPRVTRINGDLPRD
                     LLSRFESAEVEVRVKDITSDLRIPTFAAVSDDIRLRDPMLLTIGMGTHTSAGIALLRA
                     LTEVAQSRLTQIHGAREDTPTADMRKRMGYERARRMNRYWFDSPEEEQFSEVPSFDSD
                     DFLTDIRMTLDRLEGAGMDRVIVVDLTRPEINVPVVRVVVPGLEVYAMDQERMGARCH
                     AARDRRIPGPKL"
     gene            complement(3574..>3935)
                     /locus_tag="PKL26_02735"
     CDS             complement(3574..>3935)
                     /locus_tag="PKL26_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNS82642.1"
                     /translation="VPGFFRFLTIPFDPSMGGQALRMLAFETPESQFYQEPFPGFWGA
                     VHLLFWCAWININVGIFNAIPMVPLDGGYILKEGVERLFERRGLSKYALPVVSFISSL
                     MLVMLFSIIFLPYFLHA"
BASE COUNT          682 a         1198 c         1206 g          749 t
ORIGIN      
        1 atcctaggag actgggggtc ttctgacctg cgttcaaatc gcagtggccc cattttatat
       61 cgttctctcg gagtgtccat gaaatttgat gcacgctgtt ttgactgcct cctctcccgt
      121 gtctcgctgg aatgtcgcct ctgtggcgcg gaggaacacc tctcggcaga gataagggat
      181 gagtgcgccc ggctcctcga tgcgctgcgc acgcaacccc tctcgcatcc ccagatcgcc
      241 agtgccatgc accggcacgt ctatgcccgt ctcgggacag acgacccgtt ccggggactc
      301 aaggaggaga gcacccggca ggccctcgag gtgtgcagga tggtccggcc acgcattcgg
      361 agtttccgcg accatgtcct tgcaagcgtc attggaaaca ccttcgacta cggggtcaag
      421 tcccacacag tgaaggatga cttttcgcag tatttcgagg aggagttcca gtccggcctc
      481 gcgatagacg attgcgaccg gattcttccg ctcacatcgc gtgtcgtgta tttcacggac
      541 aactgcggcg agatcgtctt cgaccggctg ctgctcgagt tcctccacag gaatggggcg
      601 agagtcacgc tggcagtccg cgaggaacct atcctgaatg atgccacgca gaacgaggcg
      661 ctcgcgctgc acatggaccg tttcgccgac gtgattaccc ataccggctg cgggtgcgag
      721 ctcggcgtgc gtcttgactg catgccggat ccccttatgc aggcaatgga ggattgcacc
      781 ctggtcatct ccaaggggat ggcaaactac gagtcgctct ctgagttccg ggacctcccc
      841 cccatcgcct accttatggc ggtcaagtgc gagcccatcg ccgacgaagt gggggtgccg
      901 cgggggagca aggtcgcgat gctccggttt cagtgaagat tttccttcga atgggagggg
      961 atggcccgtt tgttgggtgt cgccgggata ccaatcggta cttcctgctc ccaagcactt
     1021 gccctatcgc gctcagggga tgccgcagcg gcggggacat tagcccaagg agcgtcaccg
     1081 tctgtggagt gcggtcgggt tcaatccacg aacaggctgc ccnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnctttacaa ccccccgcag ttggggctga tgccccaact
     1261 gctctgcgat agcctgctgg cagggttgca ccggattctt ctgaatccta tctgctcccc
     1321 cgcactggcc ttatcgcgct caggggaggc cacagcggcg gggcgtcagc cccaggagcg
     1381 tcaccgtctg tggagtgcgg tcgggttcaa tccacgaaca ggctgccctg agtccagtgt
     1441 ctttacaacc ccccgcagtt ggggctgatg ccccaactgc tctgcgatag cctgcgggca
     1501 ggactacatg gggacatacc aggattcggt acaactccag cactgacccg agagtattcc
     1561 aggacctatc cccggagggg ggtttccccc tccggcgcct cccccgcaca ccccccgcag
     1621 ttggggctaa agccccaact gctcccccaa ctgcttccct agcaactctt tcgtcctccg
     1681 caatgcctcc agcgcgtcct ccctcttctg atcgaccgcg tggagacggg taaactcaag
     1741 gaacgacttc ctgaccggag caaaaaccgc actctctgcc tgctcgcaga tgtggggata
     1801 ggtccgctcc gggaagtaga gcgaacgggc tgcagagagg agcgctgcag catcgtcgtt
     1861 ggtgaggatg ccctcatccc tggccttcct gatggtgcag cggatattga tgaggggctc
     1921 ggagaggggg gtatagtctt cggggtcata gacgagggcg acctcgtcgt cggagatgag
     1981 gaccccgtcc cggtacaggc ggtagatgag tcccacccct tccatcccca gggtgtcgag
     2041 ttccgcggcg cggagcgccc ccatgcttga ggcaccaatc accctgatgc cggatttgag
     2101 cgccgccagg atctcccggt ggccgacggc gctctcctgg aaaaagaccc cgtcgataag
     2161 ggtgatgatg cgggcgcctt cctccgcggc gtcggtcagg tcgccccgtg cggcgggcgg
     2221 gtggaagaac cgggccggga ggatggtctc cgcgactgcc cgttcacagc ttgggcccag
     2281 gaatacggcg atctcttgcg gcatggcacc gtgcccccat acgttcctgg tccatggcat
     2341 agacctcgag gcccggcacg accaccctta ccaccgggac attgatctcc gggcgggtga
     2401 ggtctaccac gatcacccgg tccatcccgg cgccctcgag gcgatcaagg gtcatcctga
     2461 tgtcggtcag gaagtcatcg ctgtcaaaag aggggacctc ggaaaattgc tcttcttcgg
     2521 gcgagtcgaa ccagtaacgg ttcatcctcc tcgcccgctc atatcccatt ctcttgcgca
     2581 tgtccgctgt cggggtgtcc tcccgggcgc cgtggatctg ggtcaggcgg ctctgggcga
     2641 cctcggtcag cgcacgcagg agcgcgatgc ctgcgctcgt gtgggtcccc atcccgatgg
     2701 tgaggagcat cgggtcccgc agacggatat cgtccgagac cgcggcgaag gtggggatgc
     2761 gaaggtcgct cgtgatgtcc ttgacccgca cctccacctc cgccgactcg aaacgggaga
     2821 ggaggtctct gggcaggtcc ccgttgatac gggtgacccg gggccccgtg ttcctgctcg
     2881 cctccgcaag cgaccacgcg tcccgctcta tcacctccat gagggcgtgg aagactgcct
     2941 cctcgagggt gtttccggac gcaagcccgt ttgtcgaggt ccggaagagc tgggggtacg
     3001 gggctgggag ggggtggaag acggcgcttg ccgggaccat gacatcctcc tcctgcagga
     3061 ggtcgtaccc ctccacccag ggaatcggca tcatggggtc cgcatttcgc gggagcacga
     3121 gaagcagggg gtcgaggaca gtagcgtccc ttcgaacctc ctcgtaccgg tcgatccgga
     3181 gttctgcgcc ctcgggaacc tccgccgagt accgctcgat cccctccatt atggccgaga
     3241 cccttgcctc ggtcggggtg gcgcccttcc cgttgtagac tgagatcgca cccctccctg
     3301 ccgtcgggcg gatgctcgag aagacaggga tcccgacccg atcgaggctc gtgatgtccg
     3361 cgaccctcgt gatccctgca gccgggagcg ccttttcaac ccgtgcgagg gtctcctccg
     3421 gagaggtggt ccgctgcgtc tcgttcttgt aggctttctt gcagggcgag aggatcatgg
     3481 agggcgtccg ttcctgttgt tgcaggttct aggtttggga gaaactcaca tataggttcc
     3541 ttgggaacgc ctcctgcagg ggcgcccctg gcgttacgcg tggaggaagt aggggaggaa
     3601 tatgatggag aagagcatca ccagcatcag ggaggagatg aaggatacta ccggcagcgc
     3661 gtatttcgaa agccccctcc gttcaaaaag cctctccact ccctccttga ggatatagcc
     3721 gccgtcgagg gggaccatcg ggatggcgtt gaagatgccg acgttgatgt tgatccatgc
     3781 gcaccagaag aggaggtgca ccgctcccca gaaccctgga aacggctcct ggtagaactg
     3841 cgattcggga gtctcgaagg ccagcattcg gagcgcctgg cctcccatgg aagggtcgaa
     3901 gggaattgtc aggaaccgga agaacccggg gaccg
//
LOCUS       DAOQVJ010000085         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_25976_length_3931_cov_3.347781, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000085 DAOQVJ010000000
VERSION     DAOQVJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25976_length_3931_cov_3.347781"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKL26_02740"
     CDS             complement(<1..1477)
                     /locus_tag="PKL26_02740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B DNA-directed DNA polymerase"
                     /protein_id="HNS82643.1"
                     /translation="MWILDTCSRNGQVEIWERGRGGRHHVVPAPSSFLLHLPDPHAHG
                     DLLEGLESRFPVEECSIRTVYGTLDGYRVQAGREAAALVEKQTRYEARIFNADLRPDQ
                     CVLAAGGLVPCSDEGESRFSTDLSFDLPAMEIAVADNPFARDPVTTLSLSVDGRQVWV
                     GGDERTILSDMEGLVRSVDPDVILFSQADIWVPRLAESARSHGLDTGLSRTGRFRRLQ
                     AKSYWSYGKTEFRVGALIPDGRLLVDTRQSFHYRESGLSGILLGARLSGLSPNLAARF
                     TSGTLISSYEVYEALRRGIAVPWRKDDPEVVRRFPELKAADRGGMMFQPVPGIYTGVH
                     EIDFTSLYPSLIVRDNLSPETLADPGKEGFLPRVLAPLIAMRMETKQKKASDPRYAGL
                     DAILKWMLVTCFGYTGYRNAKFGRIEVHEAITGSAREVLIRTRDIAEEMGFYVLHGIV
                     DCLWVQGGPVTAFKERVERETRLHAVVDAYDWLVFLPMADGS"
     gene            complement(1464..1706)
                     /locus_tag="PKL26_02745"
     CDS             complement(1464..1706)
                     /locus_tag="PKL26_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82644.1"
                     /translation="MGRSFASVRMGVREVLSRWERAARALPGEDREHALRVVAMARVH
                     ASECFYAFGDPLEATLFSVLLEVAKEQEGGRRRVDP"
     gene            complement(1708..2238)
                     /locus_tag="PKL26_02750"
     CDS             complement(1708..2238)
                     /locus_tag="PKL26_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82645.1"
                     /translation="MEFDLHPAVTLRSGTFSAVVAPGEVVLAGLNDNPVLRRFLFLFV
                     SGNCSRLLSGINRRSVSIEVRRAFTAFQLLQIIRESYHTIVFVEHDPSLYEGAGEEMA
                     PQVGEGLRQLGHGSLVVLYAEFPDPSFEAMARLADRVFYLASPAARPPAPRARGRGRE
                     RYPGGLPRGQQTLGGV"
     gene            complement(2518..3342)
                     /locus_tag="PKL26_02755"
     CDS             complement(2518..3342)
                     /locus_tag="PKL26_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82646.1"
                     /translation="MQVTNYRFPPLILVLTALLLIAFPASAQTAGTYAAEGDELSDQG
                     KFEDALVKYDLAITMEPKLAFAWCGKGLALYNLTRYDEALDAFDQAIAINPNYAKAQY
                     GRGLVLYELGRYDDAIASYDIALSIYPEYAYLVYYGKARAYAAKGDYSQAIPLFDQAL
                     ALQPSKYPGVWVEKGDAMAASGDSQGALAAYQTALALEPGFALAEAGVAAVQGDKSGG
                     VVVTTAIPQATGTPSPQTTTVHTTAPAATRTKAPLSSLTCGAGLLLAAGLALYRRH"
BASE COUNT          752 a         1235 c         1248 g          696 t
ORIGIN      
        1 cggacccgtc ggccatcggg aggaagacca gccagtcata ggcgtccacc accgcatgca
       61 ggcgggtctc ccgctccacc cgctccttga acgcggtaac cggccctccc tgcacccaga
      121 ggcagtccac gatcccgtgc aggacgtaaa agcccatctc ctcggcgata tccctggtcc
      181 ggatgaggac ttcgcgggcg ctgccggtga ttgcctcgtg cacctcgatc ctcccgaact
      241 tcgcgttccg gtagccggtg tatccaaagc atgtcacgag catccacttg aggatggcat
      301 caagacccgc ataccgcggg tcgctggcct tcttctgctt ggtctccatc cgcatggcga
      361 tgaggggggc cagcacgcgt gggaggaacc cttctttccc cgggtctgcc agggtctcgg
      421 gggagaggtt gtccctgacg atgagggatg ggtagaggga ggtgaagtcg atctcgtgca
      481 ccccggtata gatcccgggg accggctgga acatcatccc tccccggtcc gccgccttca
      541 gttccgggaa ccgccgcacc acctcagggt cgtccttcct ccatgggact gcgatacccc
      601 tgcggagcgc ctcgtagact tcgtaggacg aaatgagggt cccggaggtg aaccgggcgg
      661 cgaggttcgg ggagagcccc gagaggcgtg ccccgagcag gatgccggag agcccgctct
      721 cccggtagtg gaagctctgg cgggtgtcaa ccagcagccg gccgtcgggg atcagggccc
      781 ccacccggaa ctccgtcttc ccatagctcc agtaggactt tgcctggagc cgccggaacc
      841 tccccgtccg ggagaggccg gtatcaagcc cgtgactgcg ggcgctttcc gccagccggg
      901 gcacccagat atcagcctgg gaaaagagga tcacgtccgg gtcaacggag cggacgaggc
      961 cttccatgtc cgagaggatg gtcctctcgt cccccccgac ccacacctgc ctcccgtcga
     1021 cagagagcga gagcgtggtg actggatctc tggcaaaagg gttgtccgcg accgcgatct
     1081 ccattgctgg gagatcgaaa gagagatcgg tggagaaacg cgactctcct tcgtcgctgc
     1141 aggggacaag gccacctgct gccaggacgc actggtccgg cctgagatcg gcattgaaga
     1201 tcctcgcctc gtaccgcgtc tgcttctcta cgagtgctgc ggcctccctg ccggcctgca
     1261 cccggtaccc gtcgagggtt ccgtacacgg tgcggatgct gcactcttcc acggggaagc
     1321 ggctctccag gccctccagg agatctccgt gggcatgcgg gtccgggagg tgaaggagga
     1381 aggaggaagg agcggggacg acgtggtgcc ggccgccgcg gcccctctcc catatctcca
     1441 cctgcccgtt ccgggagcag gtgtcaagga tccacacggc gcctcccccc ctcctgctcc
     1501 tttgcgacct caaggaggac cgagaaaagc gtggcctcga gcgggtcgcc gaatgcgtaa
     1561 aagcactcgc ttgcatgcac ccgcgccatc gcgaccaccc ggagggcgtg ctcccggtct
     1621 tctccaggga gggcgcgggc ggcccgctcc cagcgggaga ggacttcgcg gacccccatc
     1681 cgcacgctgg caaaactcct ccccatctca cacccccccg agggtctgct ggccgcgggg
     1741 gagcccgccg gggtagcgct ccctcccccg ccccctcgcc ctgggcgccg gaggccgtgc
     1801 cgcggggctc gccaggtaga agacccggtc cgcgagccgc gccatcgcct cgaacgaggg
     1861 atctgggaac tcggcataca gcacgacgag cgacccatgc ccgagctgcc ggaggccctc
     1921 tcccacctgg ggggccatct cctcccccgc accctcgtag agcgaggggt cgtgctcgac
     1981 gaaaacgatg gtgtggtagg actcgcggat gatctgcagg agctgaaagg cggtgaacgc
     2041 ccgccgcacc tcgatgctga ccgacctgcg gttgatcccc gagaggagcc gtgagcagtt
     2101 cccgctcacg aagaggaaca ggaaccgcct gagcaccggg ttgtcgttta acccggccag
     2161 caccacctcc cccggcgcca ccacggcaga gaaagtcccg ctccggaggg tgaccgcggg
     2221 gtgcagatcg aactccatgc ctgtgagact ggcacctcgc acttccttaa gggtgggaga
     2281 gcacgcgggg tgaaagtgga cccggcatct tcaaataggg gggcaaaaga ccccccttct
     2341 tcatcgtcgc ccctctcccg ggtcttcaat ctgggcaaaa acggatggaa aaatttgtgt
     2401 gcgtcggaag aagaagccat ccgcaaaaac accggagaac agctgggaat ttcggtttgc
     2461 ccggcgagac ggactggaca gggggtgaga acctgcagac tggcaccgga aaaaaggtta
     2521 gtgtctccgg tacagagcca ggcctgccgc gagcaggagc ccggctccgc aagtcaagga
     2581 agagagcggt gcttttgtcc tggtcgccgc gggggctgtg gtgtgcacgg tggtcgtttg
     2641 cggtgaaggg gtaccggtcg cctgagggat ggccgtcgtc accacgacac ctccgctctt
     2701 gtctccctgg accgctgcga ctcccgcttc tgccagggca aacccgggct caagggcgag
     2761 cgccgtctgg taggctgcga gcgcaccctg tgaatctcct gacgcggcca tggcatcccc
     2821 tttctcgacc catactcccg gatacttcga aggctgcagg gcgagtgcct ggtcgaagag
     2881 agggatggcc tgcgagtagt cgcctttggc tgcgtacgcc cgggccttgc cgtaatagac
     2941 caggtatgca tattcagggt agatactgag tgcaatgtca taggaagcga tggcatcgtc
     3001 ataccggcca agctcgtaga ggacgagccc ccgcccgtac tgggcttttg cataattggg
     3061 gttgatggcg atggcctggt cgaatgcatc aagggcttcg tcataccggg tcaggttgta
     3121 gagggcaagc cccttgccgc accaggcaaa cgccagtttg ggttccatgg tgatggccag
     3181 gtcatacttc acaagtgcat cctcgaactt tccctgatca gagagctcgt caccctctgc
     3241 cgcataggtc cctgcagtct gtgcagaggc ggggaatgcg atcaggagca gggcggtgag
     3301 cacgaggatg agggggggaa aacggtaatt tgtcacttgc atggagatat ctcctttaaa
     3361 tctgcgtgtg tggctcttgg tataaaggag cataaaggtt ttggactgca ggtacacagg
     3421 tggagtggcc tcgtcagatg cacgtggttt gagagagaaa aaggtattcc tgttgcagta
     3481 ctccaaaaga catgagcaaa aagcggaacc gaagcgttga accgttttcc cgcccttgta
     3541 tttcggcatg gaacgattga agtgtgccaa gtgccacagg gaattctact taaattccgg
     3601 atgctccatc tgtgctcccc ctccccaggg cccccatttc tgccccgagt gcggcggtgt
     3661 actccagcgg atggccccct catacttcat ctcccaggac aggtgggaga cagactaggg
     3721 ggcctgtatc cccggaactg tcatctgtgc ccccatctcc atggcgcgat ctcccctggc
     3781 ggcacaggtg cccccccgca acttttaagc agcgtgcctg aaaagccaga tgtaaaggag
     3841 gaatgcatat ggcttcaaga ctgatggagt acttcaacag gcagccgagg atcgggacgc
     3901 tcagcacctc cggaaaagac gggaaggttg a
//
LOCUS       DAOQVJ010000086         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26089_length_3917_cov_4.161574, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000086 DAOQVJ010000000
VERSION     DAOQVJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26089_length_3917_cov_4.161574"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKL26_02760"
     CDS             <1..393
                     /locus_tag="PKL26_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HNS82647.1"
                     /translation="GTAIVALVSGVARWIAFGILSLIGGKMIYEGVWEREAGWEDDCL
                     SPATILILATATSIDSLGVGLSLGFVDSAIYLITFVVGAVAFIFSCLGAVLGGALAEK
                     FGKKFEVLGGIILIGIGMWILLEHLAAP"
     gene            555..932
                     /locus_tag="PKL26_02765"
     CDS             555..932
                     /locus_tag="PKL26_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82648.1"
                     /translation="MKGMPDIYQIIYQKLEKMGVLEVRQYAVIEMPPYVPLCIDRLSD
                     DVYALSQNPEVEGVMVADPDMEIRVDHTRKTAEPLTLQSGETKRVVYTAPGRVDLKTR
                     IELSRFLDTWLSDLLDKGFIRHQ"
     gene            1083..1670
                     /locus_tag="PKL26_02770"
     CDS             1083..1670
                     /locus_tag="PKL26_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HNS82649.1"
                     /translation="MPILLTEPPFYHPAFEEAHQYITGCYRIPKRPLAIFIPCALRKP
                     YSQSPSHRLFRQTISDVFHEEDHHLVIFGTCGTVPAELELMYPFAHYQYMLGKCDDPR
                     IRDDFLEIETSRLERYLRKTTHHYMRRCAYCLGVFREAMIHASERSGVPLDLLLPSNQ
                     TIETMRDPDCPFPDGSLSMKEYMDEFRNGLISMKE"
     gene            complement(2667..>3917)
                     /locus_tag="PKL26_02775"
     CDS             complement(2667..>3917)
                     /locus_tag="PKL26_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNS82650.1"
                     /translation="VGFCIVFFGVKIHGLAKKNGYLTVQDYFVHRYESPKALRSLSAL
                     SGIVVSMIYLAGQYTAVSIVLMWIFGLPHWVALLLGAFIVTIYTVIGGLYAVTWTTLL
                     QGLILIIGVVIMAPMVIMKAGGFTHINEVMAATSPDLVQPWVGSGLFTPAYVVSFSLL
                     LMIGLACSPHVINNILAVKDSRYFGWAPLIAFLVYGAVMFLLKFGGFAGIVLVQEGIV
                     TLPDLKNAGDFVFIYGIQSAIPHIVVWGLFAVVILAAVMSTTDRLMLTIGTYFSWDIY
                     RNVFRPEASDAQVLLVSKIAVAATAIITVLLALDPPAMLVWLIWAGIGIMFATFAVPL
                     LAGLYWRRATREGALAAMALGLVSSCASGGITYFKIAVLPVHFSLYGVAVATFAMVAV
                     SLATRPVDSRILDDTYTGWYIRVK"
BASE COUNT          942 a         1085 c          981 g          909 t
ORIGIN      
        1 ggtactgcca tcgttgccct tgtttccgga gttgcccgct ggattgcctt tgggatactc
       61 tccctcatcg gggggaagat gatctatgag ggggtatggg aaagagaggc aggatgggag
      121 gatgactgtc tttcccccgc aaccatcctc atcctcgcga cggccaccag tattgactcc
      181 ctcggggttg ggctgtcgct gggatttgtg gatagtgcaa tatacctcat tacatttgta
      241 gtaggtgcgg tggcattcat cttctcctgc ctcggtgctg tgcttggggg agcccttgca
      301 gagaagttcg gcaagaaatt cgaagtcctg ggaggtatca ttcttattgg gattggaatg
      361 tggatcctgc tcgaacatct tgcagccccc tgattggcct ttctgcaggg agatcttatc
      421 aatacctcct cttcgctttc tcctgctttt tggtacaaat ttcacttttc tgaaaataaa
      481 attgcagatc tcccgccaat ccttgagaat atttatcact ccgatcctct cacctattga
      541 gcaacagagg tctggtgaag gggatgccgg atatttacca aatcatatac cagaaacttg
      601 aaaagatggg agtcctcgag gtccgccagt atgccgtgat cgagatgcct ccctatgttc
      661 cgctctgcat agaccggctc tcggatgatg tctatgccct gtcccagaac cccgaggtgg
      721 agggggtgat ggtcgctgac cccgacatgg aaatacgcgt tgatcataca agaaagacag
      781 ccgaaccgct caccttacag tcaggtgaaa caaagcgggt ggtgtatacc gctccgggca
      841 gggttgactt aaagacgcgg atcgaactct caaggtttct ggatacatgg ctctcggacc
      901 tcttagacaa aggattcatc cgtcaccagt agaaggtgtc cgctcttgtc cctgtgctgg
      961 agatcgcagt caggatgagg gagatcattc atcccgttgt gtttcagaga cagtgtgcat
     1021 atttcccatt tttgccaata ccataactgc tccgggcagg cagaaggacc cgggatcctg
     1081 acatgcctat tctcctgaca gagccgcctt tttatcatcc cgcatttgag gaggcccacc
     1141 agtacatcac cgggtgttac cgcataccaa aaagacctct ggcaatattc atcccctgcg
     1201 cattgcgaaa accctacagc cagagcccaa gccacaggct cttcaggcag acaatcagcg
     1261 acgtgttcca cgaggaagac caccacctgg tgatcttcgg cacctgcggg acagtccctg
     1321 ctgaactgga actcatgtac ccctttgccc attaccagta tatgctcggg aagtgcgacg
     1381 atcccaggat tcgagacgat ttcctggaga tagagacatc ccggctcgag aggtatctcc
     1441 ggaaaacaac acaccactac atgaggcgct gtgcgtattg cctgggggtt ttccgcgagg
     1501 caatgatcca cgcctccgag cggagcggcg tgccgctcga tcttctgctt ccctcgaatc
     1561 agaccataga aaccatgcgg gatccggact gccccttccc tgacggctcg ctctccatga
     1621 aggaatacat ggatgagttc aggaacggac tcatctcgat gaaagaatga tcgtcccgtt
     1681 gttttccccc aaaaaacgca ccatcattca ttccaggttc actttaaata aaaaacaccc
     1741 gcagtcctgt gcaggcatgt actgggatat ggtcgatcac cacggtacca tgttttttaa
     1801 atacttctcc gtttctttca ggaacatgcc ccatttctca ccagagatgt cctttttttt
     1861 ctaaataagc gcatttcggg gccctgatga ttccaaaagg tttaattatg attgctctcc
     1921 atgtaatatc aggtgtatta gtgtccaact aagtcaccga ctgaacgttc caatccatgg
     1981 tggaaaccct gccctaaaag gcagggtgga cgcctcctgt tgatgccacc atcattcctt
     2041 ttgcatatca ctccagtgga gtccataatc cattgtgcgt cactgcctgt atagaacagg
     2101 ctgatagaaa agcgattgaa aaaatgctgg tattcctatg gtaggagatg ccccgtgagg
     2161 attctttcgg acacacatgt tcggccccag gatgccgcgt ctctctcacc cgcatctctt
     2221 ccgccgtttt gggatgcagg ttcggggcgt cccggcatca tgttccttga acatacctct
     2281 tcggcccccg tgatggaatg tctcagccgc ccatggcctt tttcgccgct ctggaccatg
     2341 gactcattcc atactggaag tatgtttcct gaacatttgt tcggccccat gatgttatgt
     2401 cctgatcgct cccgtatcgt tcagccgttt ggatacggga actcatttca tcatggcatg
     2461 tatccccagt tggttcctca ctgaggcatt ccccctatag tcacaatagc ataaatagtt
     2521 tttctttttg cacgttggat atctttatat aaagtatata aatgccggga ccgcgcacgg
     2581 aaaggtggct tccacaagta ttgctcttgc cctcccaatc cccttgccat gaatcaaggc
     2641 gtccagcccc tgcagaagaa agggtgttat ttcacccgga tataccaccc cgtatacgtg
     2701 tcatcaagga tacggctgtc cacgggcctc gttgcaaggc tgaccgcaac catggcaaaa
     2761 gtggccaccg caaccccata gaggctgaag tgaaccggga gcaccgcgat cttgaaatac
     2821 gtgatcccac cagacgcgca tgacgacacg aggccgagag ccatggccgc aagagctcct
     2881 tcgcgcgtcg ctctccgcca gtacaggccg gcaaggagcg gcactgcaaa cgtagcaaac
     2941 atgatgccaa tcccggccca gatgagccag accagcatgg caggcgggtc aagcgcgagc
     3001 aggaccgtga tgattgcggt agccgcaacc gcgatcttgc tgacaaggag tacctgcgca
     3061 tcggaggctt ccggtcgaaa gacgttccgg taaatatccc agctgaagta tgtgccgatg
     3121 gtcagcatca gcctgtctgt tgtggacatc acggctgcca ggatcaccac cgcaaacagg
     3181 ccccacacga cgatatgggg gatggccgac tggataccgt agatgaagac gaaatcaccg
     3241 gcattcttca aatcaggaag agtgactatc ccctcctgga ccagcacgat gccggcaaag
     3301 cctccaaatt tcaaaaggaa catgaccgcc ccgtatacaa gaaaagcgat caggggagcc
     3361 cacccgaagt accgggagtc cttcactgcc aggatattgt tgatcacgtg gggagaacat
     3421 gccagcccta tcatcaggag gagtgagaac gacaccacgt atgccggggt gaagagtcca
     3481 ctcccgaccc atggctgcac cagatcagga ctggttgccg ccatcacctc gttgatatgg
     3541 gtaaaccctc ctgccttcat gattaccatc ggtgccataa tcaccactcc gataatgagg
     3601 atgagcccct ggagaagagt ggtccaggtg acagcgtaga ggcccccgat gaccgtgtag
     3661 attgtcacaa tgaacgcgcc aagaagcagt gctacccagt gtgggaggcc gaagatccac
     3721 atcagcacaa tgctgactgc agtatactga cccgcaagat agatcatcga gactacgatg
     3781 ccggaaaggg ccgagagtga ccgcaacgct ttcgggcttt cataacggtg aacgaagtaa
     3841 tcctgcaccg tgaggtagcc attcttcttt gcaagcccat ggatcttcac cccaaagaag
     3901 acgatgcaga acccaac
//
LOCUS       DAOQVJ010000087         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26209_length_3905_cov_3.811429, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000087 DAOQVJ010000000
VERSION     DAOQVJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26209_length_3905_cov_3.811429"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKL26_02780"
     CDS             complement(<1..256)
                     /locus_tag="PKL26_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNS82651.1"
                     /translation="MSQQLGGQPILILKEGSSRTRGRDAQGMNITAAKAVASAVRTTL
                     GPKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVE"
     gene            complement(308..1225)
                     /locus_tag="PKL26_02785"
     CDS             complement(308..1225)
                     /locus_tag="PKL26_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNS82652.1"
                     /translation="MSQERLLSTVTSVLLMAGFRVSEPFSMRPRSFDLIARHREMILI
                     IKVVSHIDSVSEEVARDLDHIARYLRGAPLIIGDRARDADLERGAVYVRYGIFAISVA
                     TLYDAFVEGTPPLVYASPGGLYVSINGEVLRELREKMNLSLGDMGTLLGVSRRTISKY
                     ESGMGTTLEVAQKIEEIFDAPLVRSIDLLEYSSLFEDVPEKEEEPAPMGFLQRIGVKL
                     HAMHRAPFQALIEISDQSILTGYGSSQKVVKRAALIGNISQVAGMHAMCVLTDYAKQK
                     KIGKTLVIGEQRLLALEDGEELIQLVSKS"
     gene            1275..2522
                     /locus_tag="PKL26_02790"
     CDS             1275..2522
                     /locus_tag="PKL26_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HNS82653.1"
                     /translation="MRIGIDDTDSPRGMCTTYLGAVLGKRLARAGIAVREARLVRLNP
                     NVPWKTRGNAAVCLVADGSPKKAFRLACNTVGELSDLGHEHTHPGVVVSGDDLPVEFY
                     RKAVQGFCTLDEAQAVLDEAGALSRGYKQKRGLIGATAAACSNFDDATYELLAYRDPA
                     SWGTPRFVERESLFSADLHTYPHTWDTVDRENDLVVCVPHTPDPVLFGIRGERPGWVA
                     LAREEVVSERPSLEQVWITNQGTDAHLVPAISPEFEEGCSYLLAGAVGSLPVTHRGGH
                     VSFTLASGYRCLTCMAYEPTRGFRGVVRGLLPGDRVRVAGSYKGGSINLEKLQVVSAP
                     PLRIARPPVCGRCGKRMTSAGTDKGYKCRRCGGRESAPEILEESRVIAPGWYEVPPSA
                     RRHLARPLCRGLRQALLSLVPCP"
     gene            2567..3496
                     /locus_tag="PKL26_02795"
     CDS             2567..3496
                     /locus_tag="PKL26_02795"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNS82654.1"
                     /translation="MQCGDPVEQVSIRAGMTVGELVSALGRAGAYNGGALAKAVSVYE
                     AMLRDDQAVKFMGLAGAMVPAGMGRIVSDLIDRGHVDVLVSTGANLTHDIIEALGCRH
                     YHGSAACSDIELREEEINRIYDVFLPNDAFIRFEEFMQEVFSGLSPVAPMKICELLAV
                     IGRALPSGILASTAKKGVPVYCPALADSMIGLQYWLFSQTRRITVDAFLDLQPLLDTC
                     FGAERAGALLIGGGVPKNFILQSMLMTPRGFDYAVQLTGDRPDLGGLSGATLDEARSW
                     GKITGSASAVTVYGDATITLPLLVAAVLERVEK"
     gene            3493..>3905
                     /gene="pyrF"
                     /locus_tag="PKL26_02800"
     CDS             3493..>3905
                     /gene="pyrF"
                     /locus_tag="PKL26_02800"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNS82655.1"
                     /translation="MTELILALDVVDRDRALAVARACAPQIDAVKIGYPLVLSAGLSI
                     ARGLSDLGLPLIADFKVADIPHTNRLIAEQVFSAGFGALICHGFAGRDSVTACVDAAR
                     AMGGRCMVVAEMSHPGALEYFSGGVAERIARMAAE"
BASE COUNT          692 a         1221 c         1216 g          776 t
ORIGIN      
        1 tctcgaccat catctttgcg gcggggtgct cgatgtccat ctcttttaag atggtgacac
       61 cgtcgtttgt gatgaccacg tcaccgatgg tgtccacgag catcttgtcc atgcccttcg
      121 gcccgagcgt ggtccgtact gcgcttgcaa cggcctttgc tgcggtgatg ttcatgccct
      181 gtgcgtcacg tccacgggtg cgtgaacttc cttctttcag gatgagaatc ggttgtcctc
      241 caagttgctg cgacattgct attctccacg tgtagcgata aaattggtta ttggttctat
      301 ataaaaatta tgatttactg acgagctgga tcagctcttc cccgtcttcc agggccaaaa
      361 ggcgctgttc cccgatcacc agggtctttc cgatcttctt ctgctttgcg tagtccgtga
      421 gtacgcacat cgcatgcatc ccggcgacct gggagatgtt gccgatgagc gcagcgcgct
      481 tcaccacctt ctgggacgac ccgtacccgg tgaggatcga ctggtcggat atctcgatta
      541 aggcctggaa cggggcccta tgcatggcat gcagcttcac cccgattcgc tgcaggaatc
      601 ccatcggcgc aggctcttcc tccttttcgg gcacgtcctc gaagagcgac gagtactcga
      661 ggaggtcgat gctcctcaca aggggggcat cgaagatctc ctcgatcttc tgcgccacct
      721 cgagcgttgt ccccatcccg ctctcatact tgctgatcgt ccgcctggag accccgagca
      781 gggtccccat gtccccaagc gagaggttca tcttctcccg cagctcccgg agcacctcac
      841 cgttgatgct cacgtagagg ccgcccggcg atgcatacac gagcggcggt gtcccttcca
      901 cgaatgcgtc gtaaagggtg gccacgctga tggcaaagat gccgtacctc acgtacacgg
      961 ccccacgctc caggtcggcg tcgcgtgccc ggtcgccgat gatcagcggc gctcccctga
     1021 ggtaccgggc gatatggtcg aggtcccgtg caacctcctc gctcacgctg tcgatgtgag
     1081 agaccacctt gattatgagg atcatctccc gatgcctggc gatcaggtcg aaactcctcg
     1141 gcctcatgct gaacggttcc gagacccgga accctgccat cagcaagaca ctcgtcaccg
     1201 tggagagaag gcgttcctgt gacatcccac taggaatgga tttagactgc cctgatataa
     1261 ataataggct gaacatgcgc atcgggatcg acgacaccga ctctccccgc gggatgtgca
     1321 ccacctacct tggggcggtg ctgggaaaga ggctggcacg tgccggcata gcggtcaggg
     1381 aggcaaggct ggtgcgcctg aacccgaatg tcccctggaa gacaaggggg aatgcagccg
     1441 tgtgcctcgt cgccgacggg tcgccaaaaa aggcattccg tcttgcctgc aacaccgttg
     1501 gggagctttc ggaccttggg cacgagcaca cccatcccgg ggtggtggtc tccggcgacg
     1561 acctgcccgt ggagttctac cggaaagcgg tgcaggggtt ctgcaccctt gatgaggccc
     1621 aggcagtcct cgacgaggca ggggcgcttt ccaggggtta caagcaaaag cgtggactga
     1681 tcggagcgac cgccgcggcc tgcagcaatt tcgatgacgc cacgtacgag ctgctggcgt
     1741 atcgcgatcc cgcgagctgg ggcactccgc gttttgtcga gagagagagc ctcttttcgg
     1801 cagacctcca tacgtatccc catacctggg acactgtcga tcgtgagaac gatctcgtgg
     1861 tctgcgtccc gcacacccct gacccggtac tcttcggaat acggggggag cggccagggt
     1921 gggtggcgct cgcccgggag gaggtcgtct ccgagagacc ttcactcgag caggtctgga
     1981 ttaccaacca ggggaccgat gcccacctcg tccccgccat ctcccccgaa ttcgaagagg
     2041 gctgctcgta tctcctggcc ggggcagtcg ggtccctgcc cgtgacccac cggggcgggc
     2101 acgtctcctt cacgctggcc tccgggtacc gctgcctcac ctgcatggcg tacgagccca
     2161 ccagaggatt ccgtggcgtg gtgagggggc ttctccccgg ggaccgggtg agggtcgcgg
     2221 ggagttataa aggcgggagc atcaacctcg agaaactcca ggtcgtctct gcccccccgc
     2281 tgcggatcgc caggcccccg gtatgcgggc gttgcggaaa gcggatgacc agtgcgggga
     2341 cagacaaggg ttacaagtgc cggaggtgcg gggggagaga gagcgcgccc gagatcctcg
     2401 aggaatcgag agtcatcgca ccgggatggt acgaggtgcc cccaagcgcc cgccgtcacc
     2461 tggcccggcc cctctgcagg gggttgcgac aagccctcct ctccctggtg ccctgcccct
     2521 gaaccagacg aaggaatatt ctcttcacta ccccatatcc tgtaccatgc aatgcgggga
     2581 tccggtcgag caggtctcga tccgcgcggg gatgactgtc ggggagcttg tcagcgccct
     2641 cgggcgggcg ggggcctaca acgggggtgc gctggccaag gcggtttcag tctacgaggc
     2701 catgctaagg gacgaccagg cagtaaagtt catgggcctt gcaggggcga tggttcctgc
     2761 cgggatggga aggatcgtaa gcgatctcat cgatagaggc cacgtcgacg tcctggtctc
     2821 caccggcgcg aacctcactc acgacatcat cgaggcgctg ggatgcaggc attaccatgg
     2881 atctgccgca tgctccgaca ttgagctccg tgaggaggag attaaccgga tctacgacgt
     2941 gttcctcccg aacgatgcgt tcatacggtt cgaggagttc atgcaggagg tcttctcggg
     3001 tctctcacct gtcgccccga tgaagatatg cgaactcctc gccgtcatcg gccgcgccct
     3061 tccgtctggg atccttgctt ctacggcgaa gaagggggtg ccggtatact gtccggctct
     3121 cgcggactcg atgataggcc tccagtactg gctgttctcc cagacccgcc gcattaccgt
     3181 ggatgcattt ctcgacctcc agccgctcct cgacacgtgc tttggggcag agagggccgg
     3241 cgccctcctc atcggggggg gagtccccaa gaacttcatc ctccagagca tgctgatgac
     3301 accccggggg ttcgactacg cggtgcagct gaccggggac cgccccgacc tcggcgggct
     3361 ctctggggcc accctcgacg aggcgcgttc gtggggaaag atcaccgggt cggcgagtgc
     3421 cgtgacagtc tacggggatg cgaccatcac gctccccctt cttgtcgcgg cagttcttga
     3481 gagggtggag aaatgacgga actgatactc gcactcgacg tggtggaccg ggaccgtgca
     3541 ctcgctgtcg caagggcatg cgcccctcaa atcgacgcgg tcaagatcgg gtatcccctc
     3601 gttctctcgg cagggctgtc catcgcacgc gggttatcgg acctcggcct tcccctcatc
     3661 gccgacttca aggtggccga tatcccccac accaaccgtc tgatcgccga gcaggtcttc
     3721 tctgccgggt tcggggcatt gatctgccat ggctttgcgg gcagggactc ggtcactgcc
     3781 tgcgtcgacg cagcccgggc catggggggc cggtgcatgg tggtcgcaga gatgagccac
     3841 ccgggggcgc tcgagtattt ctcaggcggg gttgccgagc ggatcgcccg aatggcagcg
     3901 gaatg
//
LOCUS       DAOQVJ010000088         3899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26263_length_3899_cov_3.138137, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000088 DAOQVJ010000000
VERSION     DAOQVJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26263_length_3899_cov_3.138137"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1462
                     /locus_tag="PKL26_02805"
     CDS             185..1462
                     /locus_tag="PKL26_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HNS82656.1"
                     /translation="MRFGSSGIRALYGSELVTTALALGRVLGRRHEVVVVGRDTRTTS
                     RIVENAFSAGVLSEGGAVRSAGLVPTPAVAGAVPVSGVGCMVTASHNPEEYNGLKVFN
                     PGGSSLLKSQQEETERLLCEEWKGKGLSLPPPRFGTLTGTGILDSYIGGIIGEVHCAR
                     EARMVLDCGNGAGSVASPEILKRIGVDQKCINCCPSGHFSRPSEPLEEHVKYIGPLVR
                     RWGADGAVVHDGDADRMMAFDSRGRYISGDRLLVLFTRFLGAKKVVTTTDASMAIEEW
                     AEVKRTPVGDSYVSEELVRGGDLGGEPSGAWIFPRHSLCPDGPYAAAVFCRMVSEWDI
                     AAELDAIPMYPILRASLPCKSATDVMRALGATVPTDGIRVEEEDGWYLVRASGTEPKI
                     RVTAEGRTRAAAEALLGKGLTAVKQWKKEITRQ"
     gene            1475..2674
                     /locus_tag="PKL26_02810"
     CDS             1475..2674
                     /locus_tag="PKL26_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS82657.1"
                     /translation="MQCVILAAGEGKRMRPLTATRPKVMLPLANRPMLEHLVLAVRDA
                     GISSILIVTGYMEAEIRSHFGNGHALGVGIEYAVQRHQRGTADALLQAREWLEGDFIV
                     MNGDMVVPSADIASVLAADPHCMAVASSDHPEDYGVVQIEGEIITGLQEKAQSPQGNM
                     VNAGLYHFSQDIFPLLDRVWLSPRGERELTDALAMLIRKGELAACRLTSWIDVGYPWD
                     LLSANERMMQEMTASIQGVIEEGVVLKGAVSVGEGSVIRSGTCIEGPCSIGRDCRIGP
                     HAYIRGATTIGDGCHIGHATEVKNSIVLPNTNLPHFNYLGDSVVGSGCNFGAGTKVAN
                     LRHDRAEVKVRGISTKRRKLGAIVGDRVQTGINCSVNVGAVIGCGAQIGPHALVDGYI
                     APGAKIR"
     gene            2685..3842
                     /locus_tag="PKL26_02815"
     CDS             2685..3842
                     /locus_tag="PKL26_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS82658.1"
                     /translation="MQAVILAAGEGKRLRPLTRSRPKALIPVGNVPVMDYVIDALLAS
                     GIRDIIVVVGYRKEQVIRHLNSLDASVEVVVQEKQLGTAHALLQAKDLIKGRFMVLPG
                     DNFISSASIARIRDVRNALLVKEHPYPSNFGVVVTRRGYVTSILEKPAHAPSFTISTG
                     IFSLDRDFFSCIQGNDLTDAVGAFISSGKKIRAVPADDWQDAVYPWDLLQMNQKLIHA
                     ASAEKAGEVSGSCVIQGQVRIGKGSRIGPGTCITGPVVIGPGCEIGPHACIMPFSSIG
                     SRVRVEPYTMVENAIVMDDTHIGSHSRIKDTVIGEGTRLEDHVSTVTAAPLMEIQGEQ
                     IRGRFGAILGEKVRAGPFTVFEGAIAGNGVTVQGGSRIQSTMAYEDGGLVI"
BASE COUNT          773 a         1099 c         1332 g          695 t
ORIGIN      
        1 gatgctggtc gccggcgagg ggctgatctc cccggaaggt cgctcgctcc tggaaggtcc
       61 ggaccgcgac agggcactcc tcgtaggccc gtccacgtcg ggggtcgcaa gcctcgagaa
      121 actgcgccac tggtgccctt acggaaaagg ataactctat ttctcccgtt tccgattcat
      181 atccatgcgg ttcggctctt ccggaatccg ggctctttac ggaagcgaac tcgtcaccac
      241 cgcgctcgcg ctcgggaggg ttctcgggcg gcgtcatgag gtggtagtgg tggggaggga
      301 tacccggacc acctcccgga tcgtggagaa cgcgttttcc gcaggcgtgc tttcagaagg
      361 aggggcggtg cgctccgcgg ggctggtccc cacccccgca gtggcggggg cagtcccggt
      421 tagcggtgtg ggctgcatgg tcaccgcgtc ccataacccg gaagagtaca acggcctcaa
      481 ggtgttcaac cccggggggt cttccctcct gaagtcacag caggaagaga cagagaggct
      541 cctttgcgaa gaatggaagg ggaaagggct ttctttgccc ccgccgcgtt tcggcacatt
      601 gaccggtacc ggcatccttg actcctacat tggaggcatc atcggcgagg tgcactgcgc
      661 gcgcgaagca aggatggtgc ttgattgcgg gaacggggcc gggagcgtgg ccagcccgga
      721 aatcctcaag agaattggag tcgaccaaaa atgcatcaac tgctgcccgt cgggccattt
      781 ttccaggccc tcggagccgc tcgaggagca cgtaaagtac atcggccccc ttgtgaggag
      841 atggggtgca gacggggcag tggtccacga cggcgatgcc gaccgcatga tggcatttga
      901 ctcgcggggc cgctatatct ccggcgaccg gctgttggtc ctcttcaccc ggtttctggg
      961 tgcaaaaaag gtggtgacca ccaccgatgc gagcatggcg atcgaggaat gggcggaggt
     1021 gaagcgtacc ccggttgggg acagttatgt ctcggaggaa ctggtccggg ggggagatct
     1081 cgggggcgag ccgtcgggtg catggatctt tccacggcac tccctctgcc cggacgggcc
     1141 gtacgccgca gccgtattct gccggatggt ctctgaatgg gacattgcgg ccgagctcga
     1201 tgccatcccc atgtacccca tcctccgggc ctctcttccc tgtaaaagcg cgaccgacgt
     1261 gatgagggcg cttggagcaa cggtccccac cgacggcatc cgggtcgagg aggaggacgg
     1321 atggtatctc gtgcgggctt ccgggaccga gccgaagatc cgggtcacgg cggagggcag
     1381 gacgcgtgcc gcggcggagg ctctcctcgg gaaaggtctc actgccgtga agcagtggaa
     1441 aaaggagata acgcggcagt gagaggttat ctgtatgcaa tgcgtcatcc tggctgcagg
     1501 agaggggaag aggatgcggc cgctcaccgc gacccggcca aaagtcatgc tcccgctcgc
     1561 aaaccggccc atgctggagc acctggtcct ggcggtccgc gacgcgggga tctccagcat
     1621 cctcatcgtc acggggtaca tggaagccga gatccggagc cattttggga atggccacgc
     1681 actcggagtc ggcatcgagt acgcggtcca gcgccaccag cgcggaacgg ccgatgcgct
     1741 tctccaggcc agggagtggc tcgaagggga cttcatcgtc atgaatggcg atatggtggt
     1801 ccccagtgcg gatatcgcga gcgtccttgc cgctgacccg cactgcatgg cggtcgcatc
     1861 ttcggatcac ccggaggatt acggggtggt ccagatagag ggtgagataa tcaccggcct
     1921 gcaggagaag gcccaatccc cccaggggaa catggtgaac gcgggattgt accacttcag
     1981 ccaggatatc tttcccctcc tcgacagggt ctggctctcc cccaggggcg agcgtgaact
     2041 caccgacgcg ctggccatgc tgatccggaa aggtgaactt gccgcgtgca ggttgacgtc
     2101 gtggatagac gtgggctatc cctgggacct cctctctgca aacgaacgga tgatgcagga
     2161 gatgaccgcc tcgatccagg gcgtgatcga agagggcgtg gtgctgaagg gcgcggtctc
     2221 ggtcggcgaa ggatcggtaa tcaggtccgg cacctgcatc gaggggccct gcagcatcgg
     2281 gagggactgc cggatagggc cccatgccta tatccggggg gcgaccacga tcggcgacgg
     2341 ctgccacatc gggcatgcga cagaggtgaa gaactcaatt gtgctcccga atacgaacct
     2401 cccgcacttc aactaccttg gcgactccgt cgtgggaagc gggtgcaact tcggtgccgg
     2461 gacaaaggtc gcaaacctcc ggcacgaccg tgccgaggtg aaggtgcggg ggatctccac
     2521 caagcgcagg aaactcggcg ccatcgtggg tgacagggtg cagacgggaa tcaactgttc
     2581 ggtgaacgtg ggtgcggtga ttggctgcgg ggcacagatc gggccccatg cgctcgtaga
     2641 tggatatatc gccccggggg cgaagatacg ataggtgaag caggatgcag gcagtgattc
     2701 ttgccgcggg tgaaggaaag agactacggc cgctcaccag gagcaggcca aaggccctga
     2761 tcccggtcgg caatgtcccg gtgatggact atgtcatcga cgcactcctt gcctccggta
     2821 tcagggacat catcgttgtc gtgggatacc ggaaggagca ggtgatccga cacttaaaca
     2881 gcctcgatgc gagcgtggag gtggtggtgc aggagaagca gctcggcacg gcccatgccc
     2941 tgctccaggc aaaagactta ataaaagggc gcttcatggt gctgccggga gacaacttca
     3001 tcagcagtgc ctctatcgcc aggatccggg atgtgcgaaa cgccctgctc gtgaaggagc
     3061 atccgtaccc ctccaacttc ggggtggtgg tgacccggag gggatacgtg accagcatcc
     3121 tcgagaagcc tgcccatgca ccgagtttca cgatatcaac cggcatattc tcgcttgacc
     3181 gggacttctt ctcgtgcatc cagggaaatg accttactga tgcggtaggg gcgttcatct
     3241 cttccgggaa gaagatccgt gcggtccctg ccgatgactg gcaggatgcg gtctacccgt
     3301 gggacctcct gcagatgaac cagaagctga tccatgccgc atctgcagag aaggcgggcg
     3361 aggtgtctgg cagttgcgtg atccagggac aggtccgcat cgggaagggg agcaggatag
     3421 gaccgggtac ctgcatcacc gggccggtgg tgattggacc cgggtgcgag atcggcccgc
     3481 atgcatgcat catgccgttt tcaagcatcg gctcaagggt gagggtcgag ccgtatacca
     3541 tggtggagaa tgccatcgtg atggacgaca cccacatcgg gtcacactca aggataaagg
     3601 acacggtcat tggcgagggg acgcgtcttg aagaccacgt cagcacggtc accgcggcac
     3661 ccctgatgga gatccagggc gagcagatca gggggcggtt cggcgcaata ctcggtgaga
     3721 aagtccgggc cggacctttt accgtgttcg agggggcaat tgcgggcaac ggcgtgacgg
     3781 tgcagggcgg gtcacgcatc cagtcgacga tggcttacga ggacggcggc ctggtgatat
     3841 aggcgtgtgc ggaatcttcg ggtactgcgg gaaaaagaaa gcggcgccaa tcctggtgg
//
LOCUS       DAOQVJ010000089         3898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26268_length_3898_cov_3.648192, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000089 DAOQVJ010000000
VERSION     DAOQVJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26268_length_3898_cov_3.648192"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PKL26_02820"
     CDS             <1..1398
                     /locus_tag="PKL26_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS82659.1"
                     /translation="VFILVIDTTTMEVSISALVHDLDTNASTIQTIITIYTLVKAAFM
                     LIGAKLQDFIGRKRAFLIGAAMYGIGVFTAAISQNAAMFLVGWSILEGIGTVLMLPAT
                     VTFITGTYEGKERAFAFGVWGGIAAAASIFGLIFGGYLTTNHSWRWVFALELVILLII
                     FALHRVLTETRPTESWKSFDGGGAVLSVLGLMALVFGILMVKEPSEWVIVPFLILGGI
                     ILLFGFYFWERRQIRNAKGVLVDVTILPERSFLAGNVVAIGQKFVSAGFIFIFPLFYE
                     MVTGASAYETGIALLPMTLGVVVFSILGARLSSSVEPKYVLLLGIALAGAGLAALRDV
                     FSLTTTAHDIFPGSLLFGIGLGLVLSQLTNLTLSSIRSERQTDASGIYNTTRQLGSSL
                     GTALIGIVLALGFVRGLPSGQPSPYLPGQPLLSIGISDAAINQGMEWAFVAMILVVIG
                     MFLAGLFIRKTGKIV"
     gene            1627..2517
                     /locus_tag="PKL26_02825"
     CDS             1627..2517
                     /locus_tag="PKL26_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS82660.1"
                     /translation="MHKTLVSILAIAATIWFVLSSGCMTGTQNGLASQSCPDYVNPCQ
                     GMTPVPSVSINGTPIQYADVNGVRLAYRVFGSGEPILMLNGFGATMDHFYNESFLGIL
                     ATHNQVYIYDHRGMGYSSDNDAPPTMSLYSDDAAALISALGYESMNVYGASMGSSIGQ
                     QLVIDHPLRVRKLILESATYSVRIPETRLLFELIQATALNASEPAGARREAEANLVWN
                     GTWDDLSGINKSVMLLVGTDDILTPQSAAVQIAGQIDGSWLVRFKGEPHVGSRFAPVE
                     YGETTLDFLCRNESPPYATV"
     gene            complement(3170..>3898)
                     /locus_tag="PKL26_02830"
     CDS             complement(3170..>3898)
                     /locus_tag="PKL26_02830"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNS82661.1"
                     /translation="RSSFMRMLVGGDRDAAGLLIQYADQNQQRVQQMEQLLMNCSDCD
                     PQVRVMLENQVQVLSTEQNRLAILGQQEQNDRGLFGWLFGSSGVLAGTGQGTALTDDE
                     KYWLNYMREEEKLARDVYLSLGSRWNLPIFSNIAQSEQVHMDSVKTLLDRYGLPDPAA
                     GKAQGEFTDPVLQKLHDDLIAQGSVSQVEALKAGVLIEETDINDLNTAIAATTHNDIK
                     TVYENLLQGSMNHLNAFESNLARY"
BASE COUNT          834 a         1053 c         1018 g          993 t
ORIGIN      
        1 gtgttcatcc tggtcatcga caccactacc atggaagtct ccatcagcgc ccttgtgcat
       61 gacctggata ccaatgcctc aaccattcaa accatcatca ccatttatac ccttgtgaag
      121 gccgccttca tgctgatcgg cgcaaagctc caggatttca tcgggagaaa gagagcgttc
      181 ctgatcggtg ctgcgatgta tggcatcggg gttttcacgg ccgcaatcag ccagaatgca
      241 gccatgttcc tcgtaggctg gtctatactt gaggggatcg gcacggtatt gatgctcccg
      301 gcaacggtca ctttcattac cggaacatac gaggggaaag aacgggcatt cgccttcggt
      361 gtctggggcg ggattgcagc ggcagccagc atctttggcc ttatcttcgg cggatacctg
      421 accaccaacc actcctggag gtgggtgttt gctcttgagc tggtcatcct tctcatcatc
      481 tttgctctcc atcgggtatt gacggagacc cgcccgaccg aatcctggaa gagctttgat
      541 ggcgggggag cggtcctctc ggttctcgga ctcatggccc tggtttttgg gatcctcatg
      601 gtaaaagagc cttcagaatg ggtgatcgtt ccgtttctca tattgggggg gatcatcctc
      661 cttttcggat tttatttctg ggagaggcgt cagatcagga acgcgaaagg cgtccttgta
      721 gatgtgacta tccttcctga acgttcattt cttgctggga atgttgtcgc catcggccag
      781 aagtttgtga gtgccggttt catcttcatc ttccctctgt tctacgagat ggttacaggt
      841 gcaagcgcat acgaaaccgg tatcgctctt ctgcccatga cccttggggt tgtcgtgttc
      901 tccatcctcg gcgccaggct ctcatcatcg gtcgaaccaa aatatgtgct ccttttggga
      961 atagcacttg ccggagcagg attggcagct ttgagagatg tcttttccct caccaccacc
     1021 gcccatgaca tctttcctgg aagtctcctg ttcgggatcg ggcttggcct cgtcctttcc
     1081 cagctgacca acctcaccct ctcctcgatt cggagcgagc gccagaccga tgcgtcaggt
     1141 atctataaca ccacccggca gctgggaagc tctctcggga cagcactcat cgggatcgtc
     1201 ctggcccttg gtttcgtccg ggggctgcct tccggtcaac catcgcccta cctccccggc
     1261 cagcccctgt tgtcaatcgg gatcagcgat gccgcgatca atcaggggat ggagtgggcg
     1321 tttgtggcga tgatcctggt ggtcatcggc atgttccttg caggcctgtt catccggaag
     1381 acagggaaga tagtgtgagg aggtgaaagg agaggacgag aaaaactcag ggaacgtctt
     1441 tttccagtat tgacctgctc tgactgaata aaaaatgaag catgggtggg agctacgctc
     1501 gggtcataaa tgtcaaccga ttcgtccgtg ccacacgttg cgaaagatcg gcaaacatca
     1561 acgtttgtta aatgtatgaa gctgcacaat gatctgtgta tcaaccaaag tgaggtagct
     1621 ctttccatgc acaagactct tgtttccatt ctggcgatcg ccgccaccat ctggttcgtc
     1681 ctttcgtccg gatgcatgac cggcactcaa aatggcctgg ccagccagag ttgcccggac
     1741 tatgtaaatc cctgccaggg aatgactccg gtcccgagcg tatcgatcaa cgggacgccg
     1801 atccagtacg cggatgtgaa cggggtcagg ctggcatacc gcgtgttcgg gtccggtgag
     1861 cctatcctta tgctcaatgg tttcggggcc acgatggacc acttctataa tgagtcgttc
     1921 ctcggcatcc ttgctaccca taaccaggta tacatctatg accaccgggg gatgggctac
     1981 agcagcgaca acgatgcacc cccgactatg tcactctact ctgacgatgc cgccgccctg
     2041 atatccgctc tcgggtacga gagcatgaat gtgtacgggg cttccatggg gtcctcgatc
     2101 ggccagcagc tggtcatcga ccacccgtta cgcgttcgta agttaatact ggaatccgct
     2161 acctacagcg tgcgtattcc cgagaccaga cttctcttcg aacttatcca ggcgacggca
     2221 ttgaatgctt cggagccggc cggtgcccga agagaggcgg aagccaacct ggtatggaac
     2281 ggcacctggg atgacctttc aggtatcaac aagagcgtca tgcttcttgt cggtaccgat
     2341 gatatcctga ctccccagtc ggcggcggtc cagattgccg gtcaaatcga tggttcctgg
     2401 cttgtccggt tcaagggcga accgcacgtc gggtcccggt tcgcaccggt agagtacggg
     2461 gagaccacac tcgacttcct ctgcaggaac gaatcgccgc cgtatgcaac ggtgtaaatc
     2521 tttttttttt tgagggtact ccatccattt ctgctgaaaa acaggatgtg gtatcaccct
     2581 gaaaaggtga tttttctggt atgttcaaat cccatacccc ggtcagggaa gggctacacc
     2641 aggagtgatt attcttcaat gaggcgattt ctcgttggta ttctccattt tatcctgcag
     2701 cggtcattga taagggttga atggtcgctg cagtaccaac tggtatatct ggtgggttaa
     2761 taggcttttc actataaaat tcctctaaaa aaactgaatg tcgcgaatcc aacctgttca
     2821 gggtgcatca catccgagca acaggccaca gtgaagagaa aggatagatc aggattcatt
     2881 ctccctgagg ggggagattc agcggtcagg cgctcttcgt tgggataatc cggaacgcat
     2941 ggaaggatat ccatgattat gcaggattcg ctcttgcagc actcatcatc ctgcaccttc
     3001 tgctccactg gaaattctta aaggccgttc cgtcatgctt caggaagaag gcatgcgggt
     3061 ttggtcatga gtgtttcctt tctttttgat tgaattctcc aatttttttt caatgatggg
     3121 agcaaaatgg gtagatacca taccaacaaa aaagaatata cattcttttt tagtatctcg
     3181 ccaggtttga ttcgaatgca ttgagatggt tcatggaccc ctgcagcagg ttttcataca
     3241 cagtcttgat gtcattatgc gtggttgctg caattgccgt gttcaggtcg ttgatatcgg
     3301 tttcctcaat caggacccct gccttgagtg cttcgacctg ggacacgctc ccttgagcaa
     3361 taaggtcatc atggagtttc tggagaacag gatctgtgaa ctctccctga gccttgcctg
     3421 ccgccgggtc cggtaagccg tatcggtcaa ggagggtctt tacagaatcc atgtggactt
     3481 gctcgctctg ggcgatgttc gaaaatatcg ggaggttcca gcggctgccg agcgagagat
     3541 agacatcccg ggcgagtttc tcttcctccc tcatgtagtt cagccagtat ttttcatcgt
     3601 ccgtcaatgc ggttccctga ccagtaccgg ccagcacccc cgaactgcca aacaaccagc
     3661 cgaacaaccc tctgtcattt tgctcctgct gtcccaaaat agcgaggcgg ttctgttctg
     3721 tcgacagcac ctgcacctgg ttttccagca tcacccggac ctgcgggtcg caatcggaac
     3781 aattcatgag caactgctcc atctgctgga cgcgctgctg gttctgatca gcatactgga
     3841 tcaacaaccc ggctgcgtcc cgatcaccgc ccacaagcat ccgcatgaaa gaactccg
//
LOCUS       DAOQVJ010000090         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26273_length_3897_cov_4.196512, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000090 DAOQVJ010000000
VERSION     DAOQVJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26273_length_3897_cov_4.196512"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKL26_02835"
     CDS             complement(<1..589)
                     /locus_tag="PKL26_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNS82662.1"
                     /translation="MALYTHHLPAWKRREVEEIKADAGKYALVGLVDMYGIPASQLQQ
                     IRRNLKSTAKIKMTRNTLIRHAFDEMGGPVAALSGHLSGQSALIFTDENPFRLFKTLE
                     KTKTKMAAKPGETAPEDIVVEKGPTSFKPGPIVGELQQAGIPAAIEAGKVKIRETRTV
                     VKKGEVINPKLADVLAKLNIKPMDVGLILQAALFEG"
     gene            complement(590..1234)
                     /locus_tag="PKL26_02840"
     CDS             complement(590..1234)
                     /locus_tag="PKL26_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNS82663.1"
                     /translation="MVERAKIVDAVKAAIEKAPKRKFSESVDITVNLKNIDMAQPKNR
                     IDETILLPHGTGRKVGIAVIGKGDITTQAREAGVDLVIGPEEIERLGGAPREARSVAS
                     AYRFFLAETSVMPQVGRYLGPRLGPRGRMPTPIPSGTDIKPMVERLRNSVKIRTKDKK
                     TFHVKVGSTGMPPEQIAENIDAVMRRVESVLEQGSMNIRSVYVKTTMGPAERLV"
     gene            complement(1287..1763)
                     /locus_tag="PKL26_02845"
     CDS             complement(1287..1763)
                     /locus_tag="PKL26_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNS82664.1"
                     /translation="MAEVVEVLVPGGKATAGPPLGPALGPLGINVKAVVDEINAKTGS
                     FNGMQVPVKVEVDDKKNFTVSVGIPPTTALIKKEVNVEKGSPEPNIKVVGDLPIEAAV
                     RIARMKQDDMLSYDLKHGVKEVIGTCVSMGITVDGKKPKEALSAIDAGTYDKLLVE"
     gene            complement(1866..2339)
                     /locus_tag="PKL26_02850"
     CDS             complement(1866..2339)
                     /locus_tag="PKL26_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HNS82665.1"
                     /translation="MEEFPNRIFAIKTTSKQERTVADNIKKAIETGATDIQVVAIIVP
                     DELKGYVLVETPESLPRIEQLAEKVAHARTVVRGETTLSEVAHFLVPKPVVSGISEGT
                     IVELIAGPFKGEKAVVKRIDTGKEEITVELYESVLPIPITVRGDNVRVVDKGDGN"
     gene            complement(2345..2539)
                     /locus_tag="PKL26_02855"
     CDS             complement(2345..2539)
                     /locus_tag="PKL26_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HNS82666.1"
                     /translation="MEIAKPDLKFNINEELFRKYWRILKLARTPKREEFQKIALVAAA
                     GVLIVGLIGFVIYELMLFLP"
     gene            complement(2545..3636)
                     /gene="ftsZ"
                     /locus_tag="PKL26_02860"
     CDS             complement(2545..3636)
                     /gene="ftsZ"
                     /locus_tag="PKL26_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNS82667.1"
                     /translation="MRSIVEEALTRVREDSPDISKNNEDLDQILKELRTEIAVIGCGG
                     SGSNTITRMKEEGIHGARLVAINTDAQHLSRTVADHRILIGRQRTRGLGAGSIPQIGE
                     EAALENEEEIKRAVSGCDMVFITVGLGGGTGTGAAPIVAKSAREEGALTIGVVTLPFT
                     AEGVIRMENAEAGLERLRDVADTVIVVPNDRLLEVVPKLPLYAAFKVADEVLMRAVKG
                     ITELITLPGLVNLDFADVRTVMERGGVAMIGVGESESEEKAADSVKKAIRSPLLDVDI
                     SGATAALVNVIGGPDMTMEEAEGVLQEVYNRIAPDARIIWGAQVDPNMQNKMRTMLVV
                     TGVRSPQIYGRNEKITPKVQKQFEIDFLK"
     gene            complement(3633..>3897)
                     /locus_tag="PKL26_02865"
     CDS             complement(3633..>3897)
                     /locus_tag="PKL26_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HNS82668.1"
                     /translation="VLHDLISLCVNIIRRGEITFVAGDHLTICRRRFDPDRARDLGIP
                     PGPLYGQLMNGCPVRIGDREITPEMVRTRSEKRIHIPALEKFL"
BASE COUNT          693 a         1190 c         1068 g          946 t
ORIGIN      
        1 caccctcgaa gagagcagcc tgcaggatca gccccacgtc catgggtttg atgttcagtt
       61 ttgcaaggac atctgcaagc ttggggttga tgacctctcc cttctttacg acggtccgcg
      121 tctcccgtat cttgactttc ccggcctcga ttgcagcggg aatgcctgcc tgctgcagtt
      181 cgccgacgat tggacccggc ttgaaactcg ttgggccttt ctccaccacg atgtcttctg
      241 gagcagtctc accaggcttt gcagccatct tggtcttggt cttctccagg gtcttgaaaa
      301 gcctgaacgg gttttcatcg gtgaagatga gcgccgactg ccccgagaga tgaccggaaa
      361 gtgccgcgac cggcccgccc atctcgtcga atgcgtggcg gataagcgta ttccgggtca
      421 tcttgatctt ggcggtggac ttcaggttcc ggcggatctg ctggagctgg ctggcgggga
      481 tgccgtacat atcaacaagg cctaccaggg cgtacttccc ggcatctgcc ttgatctcct
      541 ccacctctct cctcttccac gcggggaggt gatgagtata cagggccatc taaaccagcc
      601 tctctgcggg ccccatggtg gtcttgacat agaccgaccg gatattcatg gagccctgtt
      661 ccagcacgga ttcgaccctc cgcatgaccg catcgatatt ctcggcgatc tgctcggggg
      721 gcatcccggt ggatcccacc ttgacgtgga acgtcttctt gtcctttgtg cgtatcttca
      781 ccgaattgcg gagacgttcc accattggct tgatgtcggt cccggaagga atcggcgtcg
      841 gcattctccc gcgtggcccc aggcggggtc cgagatatcg tcccacctgc ggcatgacac
      901 tggtctcggc caaaaagaag cgatatgcgc tggctacgct gcgggcttca cgtggagccc
      961 cgcccagccg ctcgatctct tcgggtccga tcaccaggtc cacgcccgct tccctggcct
     1021 gggtagtaat gtcacccttg ccaatgacag cgatgccgac cttcctgccg gtaccatggg
     1081 ggaggagtat cgtctcatca atacgatttt tgggctgcgc catgtcaata ttcttgagat
     1141 tgacggtaat atctacgctc tcagagaatt tccttttagg cgctttttcg atggccgcct
     1201 tgacggcatc tacaattttt gccctttcaa ccattcattt cctccatagt gcgcgaccat
     1261 actgatcttc tatggttctc tctcgtctat tccacgagga gcttgtcgta ggtgccagcg
     1321 tcgatggcgg agagtgcctc ttttggcttc tttccatcga cggtgatacc catactcacg
     1381 caggtgccga tgacctcctt gaccccgtgc ttgaggtcat aggagagcat gtcgtcctgc
     1441 ttcatacggg caatcctgac agcggcctcg ataggcaggt caccaacaac cttgatgttg
     1501 ggttccggag atcccttctc gacattgacc tccttcttga tgagggcggt ggtgggcggg
     1561 atacccacgc ttaccgtgaa gttcttcttg tcgtcgacct cgaccttcac cgggacctgc
     1621 atgccgttga agctgccggt ctttgcgttg atctcgtcga caactgcctt cacattgatg
     1681 ccgagcggcc cgagggccgg ccccaggggt ggacctgcgg ttgccttgcc tccaggtact
     1741 aatacctcga ccacttctgc catgtctgta ccaccatgcc gtcccggatg ggaccaagcg
     1801 tgggtaagaa aggttgacct ccgcagactt aaaggtatac caggagggga aggaaatttt
     1861 ggggcttaat tcccatctcc cttgtccacc acgcggacat tgtcgcccct gactgtgatg
     1921 ggaatgggga gcacgctttc gtagagctcg acggtgatct cctcttttcc agtatctatc
     1981 cgcttgacga cggccttttc tcccttgaat ggtccggcga taagctcgac gatggtcccc
     2041 tcggagatgc cgctgacaac gggttttggc accaggaagt gtgcgacctc ggagagggtg
     2101 gtctctcccc tgaccaccgt gcgcgcgtgt gcgaccttct ccgcgagctg ctcgatacgg
     2161 ggaaggctct cgggcgtctc cacgaggaca tagcctttca actcgtccgg gacgatgatt
     2221 gcgaccacct ggatatcggt ggctccagtc tcaattgcct tcttgatatt gtcggcaact
     2281 gtcctttcct gcttggaggt cgtctttatg gcaaaaatcc ggttggggaa ctcttccata
     2341 ttcatcaggg gagaaagagc atcagctcgt agatcacaaa tccgatgagg cccacgataa
     2401 ggacccctgc cgcggcgacg agcgcgatct tctggaactc ctcgcgcttc ggggtcctcg
     2461 caagcttgag gatcctccag tacttcctga agagctcctc gttgatattg aatttcaaat
     2521 ccggcttggc aatttccata gcactcactt caaaaagtca atctcgaatt gcttctgaac
     2581 ttttggggta attttttcat tcctaccata tatttgtggc gaccgcaccc ctgttacgac
     2641 cagcatcgtc cgcattttat tctgcatatt ggggtccacc tgcgcacccc atatgatccg
     2701 cgcatccggg gcgatgcggt tgtagacctc ctggagcacc ccctccgcct cctccattgt
     2761 catgtccggg cccccgatga cgttgacgag ggcggcggtt gcccccgaga tatccacgtc
     2821 aagcagcggc gagcgaatgg cctttttcac agagtccgcg gccttctcct cgctctcgct
     2881 ttctcccacg cctatcatgg cgacgccgcc ccgctccatg acagtgcgga catctgcaaa
     2941 gtccaggttc acgagtcccg ggagggtgat gagttccgtg atccccttga ctgccctcat
     3001 cagcacctcg tcggcgacct tgaacgcggc atacagggga agctttggga ccacctcaag
     3061 gagccggtcg tttgggacca cgatgacggt atctgccacg tcccttaagc gctcgagccc
     3121 tgcctcagca ttctccatac ggatcacgcc ttctgcggtg aaggggagcg tgaccacgcc
     3181 gatggtgagg gctccttcct ctcttgcgga cttggcaacg atgggagcag cgcctgtgcc
     3241 ggtccccccg cccaggccca ccgtgatgaa gaccatgtca cagccgctga ccgcacgctt
     3301 gatctcctcc tcgttctcaa gtgccgcttc ctcgccgatc tgagggatgg agcctgcacc
     3361 gaggccacgt gtgcgctgcc tgccgatgag gatgcggtgg tcagccaccg tgcgggagag
     3421 gtgctgggca tcagtgttga tggcgacgag gcgtgcgccg tgtatcccct cctccttcat
     3481 gcgggtgatg gtgttggaac cgctcccgcc gcagccgata acggcgatct ctgtccgcaa
     3541 ctccttgagg atctggtcga gatcctcgtt attcttcgag atatccggtg agtcctccct
     3601 caccctggtc agcgcttctt cgacaataga cctcatagga atttctccag tgccgggatg
     3661 tggattcgct tttcgctgcg ggtccggacc atctcgggag tgatctcccg gtctccgatc
     3721 cgtacagggc agccgttcat cagctgtccg tacagcgggc cgggaggtat tcccaggtcg
     3781 cgtgcccggt cggggtcgaa cctgcgcctg cagatggtca ggtggtcacc cgcaacgaac
     3841 gtaatttccc ctctacgtat gatgtttacg cataagctta ttaaatcatg cagtaca
//
LOCUS       DAOQVJ010000091         3894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26293_length_3894_cov_4.703569, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000091 DAOQVJ010000000
VERSION     DAOQVJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26293_length_3894_cov_4.703569"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKL26_02870"
     CDS             complement(<1..454)
                     /locus_tag="PKL26_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS82669.1"
                     /translation="MIEIRNVSRHYGTVRVFSGLSLSIPDRKAVAITGPSGCGKTTLL
                     RLIAGLERPDSGDITIAGRLASSPDYLLAPSERSIGFVFQSPALWPHMTVRENILFGL
                     WNVPGSAGEERLSDLLNTAHISHLAHRYPHEISGGEAQRVALLRAIAPR"
     gene            complement(451..2079)
                     /locus_tag="PKL26_02875"
     CDS             complement(451..2079)
                     /locus_tag="PKL26_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS82670.1"
                     /translation="MMSHRPSCSLPLPEWPWILQAALLLIFLLLIYGPLSALLIDTLG
                     FIITDPDRALEILFLSSRRIGLLLQSIGLSAAVAGTVIAGGICAGSLIWQWRTGIGRY
                     IFWAALLLIPLPLTVHALTWSTLLASAGILPGLLAPQQSVHPWLISWLVESIAFLPLG
                     ICLSTLAFELVDPDRIEAARVLCPDPRVFSRIVLPLASPLLFAGAGFLFVFCLIDYTI
                     PSIYAANVYPFEIFAEFSTSYLPAAAFLLALPLLVVTVALVLLSQRPLKSVIQDSMWT
                     RREWQPEFLLPGWFVAAQRAALGIFFLQAVLVIAVLVVLAGTSADLATTLLLAGPDSL
                     TTVAVCLGATALSLPIALALASRLVEKKRISNAWWIAATIPLAIPPPLIGISMIGFWN
                     TPGDISLFGTLAMPVFAVMFRFLPLAALIIAAQLRAVNPLLLDAASIFGKSRITNFLT
                     VYLPLILPGICIAAAGVFAFAIGELGATLLVIPPGHSTLTIRLYNYLHYGSSGSVAAL
                     GLMMTGIFLAIVSTGFLLVRRGLRTSRIHGDKPA"
     gene            complement(2085..3119)
                     /locus_tag="PKL26_02880"
     CDS             complement(2085..3119)
                     /locus_tag="PKL26_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNS82671.1"
                     /translation="MKKVILPILVLVIVAAGILVFFALPMATQPKPEVVVYTSVDQVY
                     AEPILKEYEKISGVSVKAVYDVEATKTTGLVTRLISERANPQADVFWNGEIMQTILLK
                     ENEVLTPYTSPNSTGIPPLFKDPEGYWTGFGGRFRVIIVNTNLLSPEHYPDSIDDFRE
                     GTVPADQTGIAYPLFGTTATHAAALYATLGNQAARDYFIALRDRNVRVLDGNSVVRDQ
                     VASGLLAYGLTDSDDACGAVMRGDPVDIVIPDQGPGERGTLVIPNTVALIRGGPNPGE
                     GKKLIDYLLSPDTETELLDIGWIQMATRGDHPLRCVSTTSITPMNVSYPAIAAVQEEV
                     KRDLAEIFIR"
     gene            complement(3266..3715)
                     /locus_tag="PKL26_02885"
     CDS             complement(3266..3715)
                     /locus_tag="PKL26_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82672.1"
                     /translation="MPLIIGAAVVVVIIIAAIIIVPGLISPASVNGFLPGGTIPPTQA
                     FSLEPGPVDTVPSGNNVAVQVSRDPYTGIITSLFAGGPGQKVVQAIEITVMRQDGTTL
                     SGNIQPILLSEVQLQGTRRGVDRVIVRVTYESGETYKILDRQFSFND"
BASE COUNT          924 a         1002 c         1234 g          734 t
ORIGIN      
        1 gccggggggc aatcgcccgc aggagtgcga cgcgctgtgc ctcgcctccg gatatctcgt
       61 gcgggtaccg gtgggcgagg tgggagatgt gtgccgtgtt gagcaggtcg ctcaggcgtt
      121 cttcgccggc cgaacccggg acgttccaga gcccgaagag gatattttcg cggacggtca
      181 tatgcggcca gagtgcgggg gactggaaga caaaaccgat cgatcgttcc gatggcgcca
      241 gcaggtagtc aggtgaactt gccagcctgc ccgcgatggt gatatccccg ctgtcagggc
      301 gttcgagtcc ggcaatgagc cggagaagcg ttgtcttccc gcaccctgaa gggccggtaa
      361 tggcaaccgc tttccggtca ggtattgaga gggaaagccc ggaaaacacc ctgaccgtcc
      421 catagtgtcg tgatacgttg cggatttcta tcatgcgggt ttgtctccgt ggatacggga
      481 tgttcgcagt cctcgcctga caaggagaaa tccggtgctg acaatcgcaa ggaagatgcc
      541 ggtcatcatc aggccaaggg cggcgacact cccggaagag ccgtaatgga gatagttata
      601 aagccggatt gtgagggttg aatggcccgg aggaatcacg agaagggttg cgccgagctc
      661 cccgatggcg aaggcaaaga cacctgctgc ggcaatacag atgccgggga ggatgagggg
      721 caggtacaca gtgagaaaat tcgtgatgcg actctttccg aaaatcgaag cagcgtcaag
      781 gagcaacggg ttcactgcac gaagctgggc tgcaatgatg agtgcagcaa gcgggagaaa
      841 acggaacatg acggcgaaca ccggcatcgc aagtgttcca aacagggaga tatcccccgg
      901 ggtgttccag aatccaatca tgctgatccc gatgagcggc ggaggtatgg caagcgggat
      961 ggttgcggca atccaccagg cattggagat ccgtttcttc tccacaaggc ggctcgcgag
     1021 ggccagggca atcgggaggg agagggctgt tgcaccaagg cagactgcaa cagtggtgag
     1081 cgaatccggt ccagcgagca agagggtggt tgcaaggtcg gcggaagttc ctgccagcac
     1141 cacaaggaca gcgatcacca gaacggcctg aagaaagaaa atgcccagtg cagcccgctg
     1201 tgctgccacg aaccagcccg gcaataagaa ttcaggctgc cattcccttc gagtccacat
     1261 actgtcctgg ataacggact tgagcggtcg ctgcgagagc agcacgaggg caacggtaac
     1321 cacaaggaga gggagggcaa gaagaaacgc ggctgccggg aggtaactgg tgctgaactc
     1381 tgcaaagatc tcgaaaggat agacgtttgc ggcatagatg gacgggatgg tgtaatcgat
     1441 caggcagaac acaaagagga agccggcacc ggcaaagagg agcggcgagg cgagcggaag
     1501 gactattcgc gaaaaaaccc gtggatcagg gcaaagaacc ctggcagcct cgatccggtc
     1561 aggatccacc agttcgaatg caagggtgga gaggcagatc ccgagcggga ggaacgcaat
     1621 cgactcgaca agccagctga taagccaggg atgcaccgat tgctggggag cgagaagacc
     1681 gggcagtatg cccgcagatg caaggagcgt tgaccaggtg agcgcatgca cggtcaatgg
     1741 cagggggatg agaagaagag ctgcccagaa gatgtacctg ccgatacctg tcctccattg
     1801 ccatatcagc gaaccggcac agatccctcc tgcaatgaca gtcccggcga cggccgcgga
     1861 aaggccgatg ctttgcagga ggagcccgat ccgccgggat gaaaggaaca ggatctcaag
     1921 ggcccggtca ggatctgtga tgataaagcc gagcgtgtcg atgagaagag cagagagcgg
     1981 gccgtagata aggaggagaa agatgaggag gagggcagcc tggagaatcc agggccattc
     2041 cgggaggggc agggaacacg atgggcggtg gctcatcatc gtcgttaacg gatgaatatc
     2101 tcggcgaggt ccctctttac ctcttcctgg accgcagcga tcgccgggta ggagacattc
     2161 atgggggtga tagatgttgt ggaaacacac cggagaggat ggtctccccg cgtcgccatc
     2221 tggatccagc cgatatccag cagttcagtc tcggtatccg gtgagaggag gtaatcgatc
     2281 agttttttcc cttctccggg gttcgggccg ccacggatga gtgccacggt attcgggatc
     2341 accagagtcc ccctttcacc cgggccctga tcgggaatca cgatgtccac gggatcgcct
     2401 ctcattacgg ccccgcatgc atcatccgaa tcggtgagac catatgccag gagcccggaa
     2461 gcaacctggt ccctgacgac agaattgcca tcgagaaccc tcacattgcg gtcacggagc
     2521 gcgatgaaat aatcccgcgc cgcctgattt ccgagtgtcg catagagggc agcggcgtgg
     2581 gttgcggtcg tcccaaaaag gggataggcg atgccggtct gatcggcagg gactgttcct
     2641 tcccggaaat catcgatcga atccgggtag tgttccggtg agaggaggtt cgtattgaca
     2701 ataataaccc ggaacctgcc gccaaatccg gtccagtatc cttcagggtc cttgaagagt
     2761 ggagggatgc cggtagaatt gggagaggta tacggagtca gcacctcgtt ctccttcaga
     2821 agaatggtct gcatgatctc gccgttccag aatacgtcgg cctggggatt tgcgcgttcc
     2881 gaaatcaggc gggtgaccag tcccgttgtc ttcgtggctt ccacatcgta gacagctttg
     2941 accgatactc ccgatatctt ctcgtattcc tttaatatgg gttctgcata tacctggtca
     3001 acagaggtat acaccactac ttcgggcttg ggctgggttg ccataggaag ggcaaagaac
     3061 accaggatgc ctgctgcgac aatcaccagt accaggatcg gcagaataac ctttttcata
     3121 cccttggatc ggcaggagga aatttaacaa ttctgatttg gtggtcacga cgaacgaggg
     3181 tgtatttcag agacaatgtc ccccatctcg ctctggctgt ctcctcgtgg ttctgcggat
     3241 gaaaaagatg gagatcttga aaccatcagt cattgaaact gaactggcgg tccaggatct
     3301 tgtaggtctc tcctgattcg taggtcacac gcacgatcac cctatcgacc cctctccgtg
     3361 taccttgcag ttgcacttct gacaggagga tgggctgaat attcccggag agggttgttc
     3421 catcctgccg catgacagta atttcaatcg cctgcacaac cttctggccc ggacccccag
     3481 cgaagaggga ggtaattatt ccggtataag gatccctgct gacctggacg gcaacattgt
     3541 ttccggacgg cacggtgtcg actgggccgg gctcgagaga aaatgcctgt gtcgggggaa
     3601 tggtcccacc tggcaggaac ccgttaacag atgccgggga tatcaatcca ggaacaatga
     3661 taatcgcagc gatgatgatg acgacgacaa ccgcggcacc gatgataagc ggcaatggca
     3721 gacttttttt ggatgggaca ggcgccttta tgggaggggt cgcagggccc ggcaccgttg
     3781 caggagcgct tgtcatcggt tgtgggggcg gaggcgggcc ttcagatagg accggtgcac
     3841 cgcactcttc acaaaactgc gtatcaccca cgagaggtgc tccgcatttt ccac
//
LOCUS       DAOQVJ010000092         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26478_length_3875_cov_4.339529, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000092 DAOQVJ010000000
VERSION     DAOQVJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26478_length_3875_cov_4.339529"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKL26_02890"
     CDS             <1..193
                     /locus_tag="PKL26_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS82673.1"
                     /translation="SASIRKHMKSFDLVCCAHIHEQRGVVEEKGVKVVNPGMAALGQC
                     AMVHFGTEPKEIRIELLQV"
     gene            complement(399..941)
                     /gene="cyaB"
                     /locus_tag="PKL26_02895"
     CDS             complement(399..941)
                     /gene="cyaB"
                     /locus_tag="PKL26_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNS82674.1"
                     /translation="MLEIELKVRVDDLAAVRERLCEQQAELESRVMERDLYLNAPHRD
                     FGVTDEALRIRQAGERWTVTYKGKKEPGFHLKAREELNCRVESGDVMARIFISLGFTP
                     VAEVRKWREYYHFKDATVCLDQVEGLGEFVEIELNNPGNVERPAEYVMALGEEIGVTG
                     KPILSSYLEILLAKGQGTEE"
     gene            1204..1896
                     /locus_tag="PKL26_02900"
     CDS             1204..1896
                     /locus_tag="PKL26_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS82675.1"
                     /translation="MAIKVGDFIRISYTGRVDGKVFDTTDEDIAKDAGIFNPEASYGP
                     VTIRVGSHHVILGLDEALDGKEIGEEGEVDVPPEKAFGPHEPTQVEAFNKNSFKDKPK
                     KGMSIKVPEKGEGTVVDVIGNRVLIDFNHPLAGKPLSYSFRVEDIVEGAVEQMKGLIK
                     LYAGRDMDVSLNEGALTLHLPPGITYDRRWLLWRSRVIHEGFEHIPTIDEITLVETFA
                     RPEKAEAETPEE"
     gene            complement(2118..3467)
                     /gene="purB"
                     /locus_tag="PKL26_02905"
     CDS             complement(2118..3467)
                     /gene="purB"
                     /locus_tag="PKL26_02905"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNS82676.1"
                     /translation="MAIHPIEFRYGTAEMRAVWGEEYRFSCIVQAEVALARASAAHLF
                     IPAAAAEAIASCAGKATLARAKEIEEEISHDMMAIVKAIAEQCGEGGGYVHYGATSND
                     ILDTATGLQIRAALDILDGNLRRLLAALLKRADETKMLVCAGRTHGQIGVPTTYGLRF
                     AIWASEVARHIERLGQMAPRVEVGQLTGAVGTQAALGERGMEIQATMMEYLGLTAVDV
                     SNQVVSRDRYAEYFMFLANVATTLDKIGIGIRTLQRTEIGEVEEAFGARQVGSSTMPH
                     KRNPIKSEQVCGLSRVVRSAVEPALQNNTLWDERDLTNSSCERVLFPEATILADHILA
                     LMIRVIEGLTIKPERIAQNLSVLQGVNMAEAVMIELTRRGMDRQEAHESMRLASMQAL
                     EKKVPLAKVLSSDEKVMRFLSPDEVGALLDPLHYIGTAPAQVERLIRKLAPLCRVSV"
     gene            3529..>3875
                     /locus_tag="PKL26_02910"
     CDS             3529..>3875
                     /locus_tag="PKL26_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNS82677.1"
                     /translation="MDSLDDWFPYADYRPCQREMLEFGLSCAQEGTIGLIDAPTGSGK
                     SSLLAALLAGREKRRVLVTVRTVSQLNTFIRELSLIREKKPGLKVAYLIGKRGMCPLS
                     GEGDVYRRCEGVKG"
BASE COUNT          815 a         1176 c         1104 g          780 t
ORIGIN      
        1 aagcgccagt atcaggaaac acatgaagtc ttttgacctg gtctgctgcg cccacatcca
       61 tgagcagagg ggagtcgtgg aggagaaagg ggtgaaggta gtcaaccccg gcatggcagc
      121 gctcggccag tgcgccatgg tccacttcgg cacggagcca aaggagatca ggatcgagct
      181 gctgcaggtc tgatcatccc ggcctgcggg aactgcacac accttttttt aaaaaaacta
      241 catgattctt tccgaaacac acgcatatgc attcatgaaa atggcacagc cattttcata
      301 gtacccacct attaccgcct ggcggtcgag cataattgat gaaaatcacg aagtgatatt
      361 cattcgaaaa aagttgtcaa ggagcggtgg gtccgttcct actcctctgt cccctggcct
      421 tttgcgagga gaatctccag gtatgacgag agaatcggct tccccgtgac ccctatctcc
      481 tctcccagtg ccatgacgta ttcggcaggc cgctcgacat tcccgggatt gttcagttct
      541 atctccacga actccccgag cccctccacc tggtccaggc agacggttgc atctttgaag
      601 tggtaatact ccctccactt cctcacctcg gcgaccgggg taaacccaag cgagatgaag
      661 atgcgggcca tcacgtcacc tgattccacc ctgcagttga gctcctccct tgccttgagg
      721 tggaaccccg gctccttttt gcccttgtag gtcaccgtcc agcgctctcc cgcctgcctg
      781 atacggagcg cctcgtccgt caccccgaag tccctgtggg gagcattgag gtacaggtca
      841 cgctccatca ccctcgactc aagctctgcc tgctgctcgc agagccgttc ccttaccgcg
      901 gcgaggtcat ccaccctgac cttcaactcg atctcaagca tgctctcccc ttcccatctc
      961 ccccatgagg acaataatac gtgtggtttg gaacgcgggg cgaccggcga cagctccctc
     1021 tccgggtgca gcagaattgt gacccgcaaa agaaaaaaaa ccaggcgaca ggagggtgtt
     1081 cccgggagtg ccgccgtcaa tcgattaact atttaagacc gtccccgcaa atgtataaag
     1141 aacctctacc cgggaagcgc caggattttt ctccccgggt agccggagat tccaggtgaa
     1201 gaaatggcaa taaaggttgg agattttatc agaatcagct acacgggcag ggtggacggg
     1261 aaggtctttg acacgactga cgaggatatc gcaaaggacg cgggcatctt caaccccgag
     1321 gcatcttacg gcccggtgac catccgtgtc gggagccacc atgtcatcct cggcctcgac
     1381 gaggccctcg atgggaagga gataggagag gaaggtgaag tggacgtccc gcccgaaaag
     1441 gcgttcggac cccatgagcc cacccaggtc gaggcgttca acaagaactc cttcaaggac
     1501 aagccaaaga aggggatgag catcaaggtc ccagagaagg gagaggggac ggtggtcgat
     1561 gttattggca accgtgtcct catcgacttc aaccaccccc tcgccggaaa gccgctctcc
     1621 tattctttcc gggtcgagga tattgtcgag ggtgcagtgg agcagatgaa agggctcatc
     1681 aagctctacg cgggccggga catggacgtg agcctcaacg aaggagccct caccctgcac
     1741 cttcctccag gcatcaccta cgaccgccgg tggctcctct ggaggagcag ggtcatacac
     1801 gagggattcg agcatatccc cacaatcgac gagatcacac ttgtcgagac cttcgcacgc
     1861 cccgagaagg ccgaggccga aactccggaa gaataattca acgctcacac cttttttcca
     1921 atgaaacgca catgagactt tcgtggtaca tgcccccctg atgaaaaccg tggaatgtgg
     1981 ttttcatgcc aaaatgcgtg cggcattttc atgaatgcga atgcgtgtgt cttggagaga
     2041 atcatgtagt tttttccccg gtcatcgata cgggtgaccg gaaatggcgt gaaaagaaag
     2101 ggtccctgaa ggtccgatca aacggatacc ctgcagagcg gtgccagttt ccggataagc
     2161 ctttccacct gtgcaggggc cgtgccgatg tagtgcaggg gatcaaggag tgccccgacc
     2221 tcatcagggg agaggaaacg catcactttc tcgtctgacg agaggacttt tgcaagcggg
     2281 accttctttt cgagcgcctg catgctggcc aggcgcatgg attcgtgcgc ctcctggcgg
     2341 tccatgcctc tccgggtcag ctcgatcatc actgcctcgg ccatgttcac cccctggagg
     2401 accgagaggt tctgagcaat ccgttctggc ttgatggtga gcccctcgat caccctgatc
     2461 atcagggcaa ggatgtggtc ggcgagaata gtcgcctcgg ggaagaggac ccgctcgcac
     2521 gaggagttgg tgagatcgcg ctcgtcccag agagtgttgt tctgcagggc aggctccact
     2581 gccgaccgga ccacccgcga gaggccgcag acctgctcgc tcttgatggg gtttctcttg
     2641 tgcggcatgg tgcttgaccc gacctgtcgg gccccgaacg cctcctccac ctcgccgatc
     2701 tcagtcctct gcagtgtccg gatcccgatg ccgatcttgt cgagggtggt ggcgacattt
     2761 gccaggaaca tgaaatactc cgcgtacctg tccctcgaga cgacctggtt cgagacgtct
     2821 accgccgtca gcccaagata ctccatcatt gtcgcctgga tctccattcc tctctccccg
     2881 agtgccgcct gggttcccac tgcgccggtc agctgcccga cctccacccg cggggccatc
     2941 tggccaagcc gctctatgtg ccgggcgact tcgctcgccc agatggcgaa ccgaagaccg
     3001 taggtggtgg gcaccccgat ctggccatgc gtcctccccg cacagacaag catcttcgtc
     3061 tcatcggccc gcttcaggag ggcggcaaga agcctgcgga ggttcccgtc aaggatgtcg
     3121 agagccgccc tgatctgaag accggttgca gtatccagga tatcgttcga cgtggccccg
     3181 tagtggacat agccccctcc ctcgccgcac tgctctgcaa tggccttcac gatcgccatc
     3241 atgtcgtggc tgatctcctc ctcgatctcc ttcgcccttg caagcgttgc cttcccggcg
     3301 cacgaagcga tagcctcggc ggccgcggcg gggatgaaca ggtgtgctgc cgatgctctt
     3361 gccagggcca cctctgcctg cacgatgcat gagaaccggt actcctcccc ccagactgcc
     3421 cgcatctctg cggtcccgta ccggaactcg atgggatgga ttgccatggg agaagggttg
     3481 gtgtttcccg ataataaaaa agagtcaggg ctccaatggt atggtacgat ggacagcctc
     3541 gacgactggt tcccctacgc ggattatcgc ccatgccagc gggagatgct tgaattcggc
     3601 ctctcgtgcg cacaggaagg gacgatcggg ctgatcgatg ccccaaccgg gagcggaaag
     3661 tcgagcctcc tcgcagcgct cctcgcgggg agggagaagc gccgcgtcct ggtcactgta
     3721 cgaaccgtga gccagctgaa cacgttcatc cgggagctct cgctcatcag ggagaagaag
     3781 ccggggctca aagtggccta cctcatcgga aagaggggga tgtgccccct ctcgggagag
     3841 ggggacgtgt atcgccggtg cgagggagtg aaagg
//
LOCUS       DAOQVJ010000093         3862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26603_length_3862_cov_4.843709, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000093 DAOQVJ010000000
VERSION     DAOQVJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26603_length_3862_cov_4.843709"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKL26_02915"
     CDS             <1..415
                     /locus_tag="PKL26_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNS82678.1"
                     /translation="HGLDRYRIHTPPNIPNIRLNGGAVLEEGMVFAIEPFASTGIGQV
                     SEKHRTEIYQQLAVKPVRLPSARRVLDEIRQRQGLPFSRRWLNEGKLDLALSTLVRSQ
                     VIRAYPVLSDVPGSLVSQAEHTLIVTSEGCLVTTA"
     gene            460..1737
                     /locus_tag="PKL26_02920"
     CDS             460..1737
                     /locus_tag="PKL26_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS82679.1"
                     /translation="MAADDETLKLIEILLTAELFNSHQNLDANDLTPECRNLFTVNGT
                     AEVKRPLHVSEGMVRRSLGIENACRKLEQAPVVTCEEFGQRIKLTALEPAARWFLKIG
                     GMPQIEKNPTLAFFFEHFESTKVSYEKARQDNPPIEDTRVFLQSRLTKILADDERLKN
                     AMDLIILSAPEEVEQDFNDLVCTRAQENIIRKIQVARTHRDILRRHRIHEVGKLLFVG
                     PPGTGKTSLALALARKLHLPLLEVRLSMITSQYLGETSKNIDRIFELAKALSPCILFI
                     DEFDFVAKSRVADDHGAMKRAVNSLLKNIDQVSLIRHGVLLVGATNHPQLLDEAAWRR
                     FDEVVEFGLPDREMRRAILEKVSTGMQYMFDIPDLAERTEGFSGADLRIMIKEALLSS
                     LMDHRDAINITDIERGITVVKNRDTIRSQNWLS"
     gene            1739..2458
                     /locus_tag="PKL26_02925"
     CDS             1739..2458
                     /locus_tag="PKL26_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS82680.1"
                     /translation="MQIVLLGTGDTIGTPRIGCSCTQCTHAHEHGLQRLRTSLLVERQ
                     GRHILIDSGPDLRQQLLAAGSPHIDAVLWTHGHYDHFMGFAEFYRVQDVPDVYAPLPV
                     MDYCGRIFQFLLRDRHPVRAYCPFTLFGVECTFLTVDHPPTYTCGVLLEADGVRIGYT
                     SDTTLRLPEETSKHLEGTDLLFVDALLPSHLHVPKHMNYKEACDLARTLGAGDFRCVH
                     QSHHLPWGLPHLGCDGETFTF"
     gene            2542..2817
                     /locus_tag="PKL26_02930"
     CDS             2542..2817
                     /locus_tag="PKL26_02930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HNS82681.1"
                     /translation="MNIKILELEKDKAKLLIQGEGHTFMNVLTEEILKDPDVDVAKYL
                     IKFQFSDPELLVTTNGKKAPLDAIREACQRISGSCDELLEILSKGRS"
     gene            complement(2887..3504)
                     /locus_tag="PKL26_02935"
     CDS             complement(2887..3504)
                     /locus_tag="PKL26_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HNS82682.1"
                     /translation="MVEPYETLLKKAYSHVTEPTESVERFTVPEARSYIEGKTTVLEN
                     FSEIADTVRRDKDHLMKFLLGELGTAGKIDGTRAIFNGKFEAALINSLVKGYVDDYVI
                     CSECGKPDTRLVKDDRVLLLRCDACGGHRPVRKRKARTEPVTNEISEGAILDLEVQSV
                     SRRGDGVVRMGRYILYIPGGKPGQKVKVRITRVSGSIVFTERVLV"
BASE COUNT          854 a         1147 c         1059 g          802 t
ORIGIN      
        1 ccacggactg gacaggtaca gaatacatac tcccccgaac atccccaata tccgtctcaa
       61 cggcggggct gtcctcgagg aggggatggt ctttgccatc gagccttttg ccagtaccgg
      121 gatcggccag gtgagcgaga agcaccgcac cgagatctac cagcaactgg cagtaaagcc
      181 ggtgaggctg ccgtccgccc gccgcgtgct cgatgagatc aggcaacgcc agggcctgcc
      241 tttctcaagg agatggttaa atgagggaaa actcgacctt gccctctcca ctcttgtccg
      301 ctcacaggtg atacgtgcat accctgtcct ctccgatgtc ccggggtcgc tcgtttccca
      361 ggccgagcac acactcatcg tcacaagcga ggggtgcctc gtcaccaccg catgagatat
      421 ccttaaacta tcccaggaaa aatagtaaaa gttacagaaa tggctgctga tgacgagacc
      481 ctcaagctca tagagatcct cctcaccgca gaactcttca actcccacca gaaccttgac
      541 gcaaacgacc tgaccccgga atgcaggaac ctctttacag tcaatggcac ggcggaggtg
      601 aagcggcccc tgcacgtgag cgaggggatg gtcaggagaa gcctcggtat cgagaacgcc
      661 tgcagaaagc tcgagcaggc gccggtggtc acctgcgagg agttcgggca gcgtataaag
      721 ctgactgcgc tcgagcctgc ggcgcgctgg ttcctgaaga taggcggcat gccgcagata
      781 gagaagaatc ctaccctggc ctttttcttt gagcattttg aatcgacgaa ggtatcctac
      841 gagaaggcgc gccaggataa cccccccata gaagacacac gggtgttcct gcagtccagg
      901 ctcacgaaga tactcgcgga cgacgagcgc ctgaagaacg cgatggacct gataatattg
      961 agcgcgcccg aggaggtcga gcaggacttc aacgacctcg tctgcacgag agcgcaggag
     1021 aacatcatca ggaagatcca ggttgcaaga acgcacaggg atatcctccg caggcaccgc
     1081 atccacgagg tgggcaagct cctcttcgtg ggccccccgg gcaccggcaa gacctccctt
     1141 gccctggcac tcgcccggaa actccacctt cccctcctcg aggttcgcct ctcgatgatc
     1201 acctcccagt acctcggtga gacctcgaag aacatcgacc ggatcttcga actggcaaaa
     1261 gccctctctc cctgtatcct gttcatcgac gagttcgact tcgtggcaaa gagcagggtt
     1321 gccgatgacc atggtgcaat gaagcgggcg gtcaactcgc ttttgaagaa cattgaccag
     1381 gtgagcctga tccggcatgg cgtcctcctt gtcggggcaa cgaatcaccc gcagctgctc
     1441 gatgaggctg cctggaggag gtttgacgag gtggtcgagt tcgggctccc ggacagggag
     1501 atgcgcaggg caatcctcga gaaggtctcc actggcatgc agtacatgtt cgatatcccg
     1561 gaccttgcag aaagaacgga agggttctcg ggggccgatc tccgtatcat gatcaaggag
     1621 gcgctcctct cttcgctgat ggatcaccgg gatgcaatca atataaccga tatcgagcgt
     1681 ggcatcacgg tggtgaagaa cagggacacc atccgttctc agaactggtt gtcataggat
     1741 gcaaatcgtc ctcctgggca cgggtgatac gatcggtact cccaggatcg ggtgttcgtg
     1801 cacccagtgc acccatgcgc acgaacatgg cctgcagagg ctgcggacat cgttgctggt
     1861 ggagcgccag ggccgccaca tcctgatcga cagcgggccc gacctccgcc agcagctcct
     1921 tgccgcagga tctccgcata tcgatgccgt gctctggacc cacggccact acgaccattt
     1981 catggggttt gccgagttct acagggtcca ggatgtgcca gacgtgtacg cgccccttcc
     2041 tgtaatggac tactgcggga ggatcttcca gtttctcctt cgcgatcgcc accctgtcag
     2101 ggcatattgc ccgttcaccc tcttcggggt ggagtgcacg tttctcaccg tggaccatcc
     2161 tcccacgtac acctgtgggg ttctgctgga ggccgacggc gtgcggattg ggtatacctc
     2221 cgacacaacc ttgcggctcc ccgaagagac ctcgaagcat cttgaaggaa cagacctcct
     2281 cttcgtggac gcacttctcc cttcccacct ccatgtcccc aagcacatga attacaagga
     2341 ggcatgtgac cttgcacgca cgctcggcgc aggggatttc cgttgcgtgc accagagcca
     2401 tcaccttccg tggggcctcc cacacctggg atgtgacggc gagaccttta ctttctgatc
     2461 gcgtggtgcg attcgcccgg cagatgcccg cgaggaaccc acgcatacct atataggact
     2521 cccccgccca ttcataggtg gatgaatata aagatcctgg aactcgagaa agacaaggca
     2581 aaactcctca ttcaggggga gggccacacc ttcatgaacg tcctcacaga ggagatcctg
     2641 aaggaccctg acgtggacgt ggcgaaatac ctcatcaagt tccagttctc ggaccctgag
     2701 cttctcgtca ccaccaatgg aaagaaagcc ccgctggacg cgatccgcga agcctgccag
     2761 aggatatccg ggtcgtgtga cgaactcctt gagattctct ccaaggggag atcgtaaagc
     2821 gcggaattgt tgagaaaaag gtgatttatc ctattttatt ggtattccat cctgagactg
     2881 ccaggttcag acaagcaccc gttccgtgaa cacgatggaa ccggagacgc gggtgatcct
     2941 gaccttgact ttctgacccg gctttccacc ggggatatac aggatatacc gccccatcct
     3001 caccacgccg tcgcccctcc ggctcacaga ctggacctca aggtcgagga tcgcaccttc
     3061 tgagatctca tttgtgaccg gctcagtccg ggctttcctc ttcctcaccg ggcggtgacc
     3121 tccgcaggca tcgcaacgca ggaggaggac acggtcgtcc ttcacgaggc gggtgtcagg
     3181 tttcccgcac tcggaacaga tgacatagtc atccacgtac cccttcacca gggagttgat
     3241 cagcgccgcc tcaaatttcc cgttgaagat ggcgcgggtg ccgtcaatct tgccggcagt
     3301 gccgagttcg ccgaggagga atttcatcag gtgatccttg tcgcggcgga cggtatcggc
     3361 gatctccgag aaattttcga gcacggttgt ctttccttcg atatacgacc tcgcctccgg
     3421 caccgtgaac cgctccaccg attcggtcgg ctcggtcacg tgggaatagg cttttttcag
     3481 gagtgtctcg tacggctcaa ccatagtact tatataaggt gtccaggtgt aaaaaatgcc
     3541 ccctcatggg aacggatgtt ccctcttttt cacaggcttc cgtacaggca ggctctcgtt
     3601 ctcctccttg agactcgcct gcagtgaagg gattaagggg gcaaagatcc ctgctgtcac
     3661 tttgcacaac ggatatggtc aagggttaag gcccggagcc cggtcgcgat gccagggacc
     3721 tgcatcgcct tctcaattta cgctgttctc acgcattttg acctggtaac caaaaaaata
     3781 ccttcatgtg atgattgaaa aggaataccg gttactttaa aagaaaaaga agatgggtct
     3841 ttattgtttt ttgaactggg gc
//
LOCUS       DAOQVJ010000094         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26633_length_3859_cov_2.891430, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000094 DAOQVJ010000000
VERSION     DAOQVJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26633_length_3859_cov_2.891430"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..1449)
                     /gene="scmF"
                     /locus_tag="PKL26_02940"
     CDS             complement(346..1449)
                     /gene="scmF"
                     /locus_tag="PKL26_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SynChlorMet cassette radical SAM/SPASM protein
                     ScmF"
                     /protein_id="HNS82683.1"
                     /translation="MTPEMDGGPAAYPLRMVYVYLTRDCNLACRHCWISPNYQRSCVA
                     SEYLPPDYFASILEQARELGVHTVKLTGGEPLLHPEIGKIFSCVGDLDLRLTMETNGT
                     LVTREIARSLASGKQPFVSVSLDGGDAAVHEGVRGVRGCFDAACRGIATLNDAGVALQ
                     VIMTVMRDNRDHVESVVRLAESLGAGSVKFNVLQPAGRGERIWRDGDSLTVGEYLELF
                     RWIENTLAPTTELRLFCDYPMAFRPLGRVLGREGDGCGACGIHGIIGVLADGRYALCG
                     VGETTPDLVLGSHPRDRLCTVWGDAPALRAVREGIPFNLKGVCGECVLNRLCLGSCVA
                     LNYCRSGDLLSPFWFCEEAEKQGLFPESRRIGR"
     gene            complement(1439..2608)
                     /gene="scmE"
                     /locus_tag="PKL26_02945"
     CDS             complement(1439..2608)
                     /gene="scmE"
                     /locus_tag="PKL26_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SynChlorMet cassette radical SAM/SPASM protein
                     ScmE"
                     /protein_id="HNS82684.1"
                     /translation="MPDTNDQEDGVTPLHMKVMPTPRTVDISITRRCNLRCTYCSHFE
                     SDAHIGEDLDTAEWLRFFQELGRCRVMRVTLGGGEPFCRRDLPVLLQGITENRMRFTL
                     LSNGTLITDDAAAAVARTGRCDVVQISIDGSTAESHDLFRGRGSFVRAVEGIRSLQRH
                     DVPVTVRVTIHQGNVHDLPGIMKFLLDDLKIPVVSTNAASYLGKCRQHEDTVGLSIEE
                     QVVAMEHLVQLSHAYPGRIHATAGPLADARMWREMTEAKYHTGNGQAAPGRGYLSGCG
                     CTATSLSVRSDGAIVPCVQLNHIVLGTIGTGCIEEIWLNHPQLQRLRMRRSIPLSTFA
                     ECRECPYITACTGGCPALAYTRTGELDRPSPDGCFKRFLDAGGRLPVEREGEDDA"
     gene            3070..3228
                     /locus_tag="PKL26_02950"
     CDS             3070..3228
                     /locus_tag="PKL26_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82685.1"
                     /translation="MQVPYHKFRQKMKSRCELYGLWFDDTHPKADTSQVDAFALDPIR
                     KPSYGKTR"
BASE COUNT          858 a         1144 c         1049 g          808 t
ORIGIN      
        1 ggccgcaccg caggtagcca cgcatcctgc aaggatgagg acgagcaacc cgataattct
       61 catggttccg gcggtcatat tcacaaagtg attgtatgtg ggattgaata ctgcaatcga
      121 cagatctctg ctctacgaca tgacaacgtt gcaagagaag aaatatcttt cgcatttcag
      181 tgaatctaat cgaatatagt ctatggaaaa ttactattaa tcctcatttt gcaaattttt
      241 tgccgatgta tcatacaatt atttttatct tcgcggggtt gaaggggcag agcaggaaaa
      301 ccccctgcgc aagcggtgag gcagctcacc gacattccac gcggattatc ttccaattcg
      361 ccttgattcc gggaacaatc cctgtttttc cgcttcttcg cagaaccaga acggtgaaag
      421 gagatcgccg ctccggcagt agttcaatgc cacgcagctc ccgagacaga gccggtttaa
      481 aacgcactca ccgcagacac ctttcagatt gaacggaatc ccctccctga ccgcacggag
      541 cgcgggagcg tctccccaca cagtgcaaag ccggtcgcga gggtgggaac cgagcaccag
      601 atcaggagtc gtctcgccga caccacagag tgcataccgc ccgtcggcga ggaccccaat
      661 gatcccgtga atcccgcagg cgccacaacc atcgccttcc ctgcccagga cccgcccgag
      721 cggacgaaat gccatcggat agtcgcagaa gagccggagc tcggtcgtag gtgcgagagt
      781 gttctcgatc cacctgaaga gttcaagata ttcaccgacc gtgagcgagt ccccgtcgcg
      841 ccagatccgt tctcctcttc cggcgggctg gagtacattg aacttgacgg aacctgcccc
      901 gagcgactcg gcaagccgta cgacgctctc gacatgatcc ctgttgtcac gcatgaccgt
      961 catgatgacc tgaagcgcaa cgccagcatc attgagagtt gcaatccccc tgcacgccgc
     1021 atcaaaacag ccccggacgc cgcgcacacc ttcgtgaacg gcagcgtcgc cgccatcgag
     1081 gctcaccgac acgaacggct gtttccccga ggccagactc cgcgcgatct cgcgggttac
     1141 gagcgtaccg ttcgtctcca tggtaagacg gaggtccaga tccccgacac acgagaagat
     1201 cttgccgatc tccgggtgca ggagcggctc cccgccggtc agtttgacgg tatgaactcc
     1261 gagctcccgc gcctgttcga ggatagaggc aaagtaatcc gggggcagat attcactggc
     1321 aacacatgac ctttgatagt ttggcgatat ccagcagtgg cggcaggcga ggttgcagtc
     1381 gcgcgtgagg tatacgtaca ccatcctgag cggatatgcc gccgggcccc cgtccatttc
     1441 aggcgtcatc ttctccttct cgttcgacgg gcagtcgacc tcctgcgtcc aggaaccgct
     1501 tgaagcaccc gtccggactc ggtcggtcga gctctcccgt acgggtgtat gcgagtgcag
     1561 ggcaccctcc tgtacaggcg gttatatacg ggcattccct gcattcggca aaggtgctca
     1621 gcgggatgga acgccgcatc cgcaaccgtt gcagttgcgg atgattcagc cagatctcct
     1681 caatacaacc ggtaccgatg gtaccaagca cgatatggtt gagctgaacg cacgggacga
     1741 tcgcgccgtc ggagcgaacg ctaagggatg tcgccgtgca tccgcacccg gacaggtacc
     1801 cccgtccggg agctgcctgt ccgtttccgg tgtgatactt cgcctcagtc atctcccgcc
     1861 acatcctcgc gtcagcaagc ggacctgcgg ttgcatggat cctgccgggg tatgcgtggc
     1921 tgagctggac gaggtgttcc atggcaacta cctgttcttc gatggagagg cctaccgtgt
     1981 cctcgtgctg ccgacattta ccgagatacg aggccgcatt cgttgagacc acaggtatct
     2041 tcaggtcgtc cagaagaaac ttcataatcc cgggaagatc gtgtacattt ccctggtgaa
     2101 tggtcacccg gaccgtaacc gggacatcat gccgctggag ggagcggatt ccctcgacgg
     2161 cccggacaaa cgaccccctg cccctgaaca gatcgtggct ctccgcagtc gacccgtcga
     2221 tcgagatctg gacgacgtca cacctgccgg tccgggctac cgctgctgcg gcgtcgtcgg
     2281 tgatcagggt tccgttcgag aggagggtga accgcatccg gttctcagtg atgccctgga
     2341 ggagaacggg aagatcccgg cggcaaaacg gttcgccgcc gccgagggta accctcatca
     2401 ccctgcagcg tcccagttct tgaaaaaaac gaagccattc ggctgtatcc agatcttctc
     2461 cgatatgagc atcgctctcg aagtggctgc agtaggtaca cctcaaattg caccggcggg
     2521 tgatcgagat atcgacggtc cggggggtcg gcatcacctt catgtgcagt ggtgtcaccc
     2581 catcctcttg atcgtttgta tccggcatgc cctttctccg tgagcctttc ggcaccggcc
     2641 gcaattcttc gagaacgtgg ctttttcccg gcagattctg aagaagagcg gcttttcccc
     2701 tgaagacggg tgcggtaccg ggtacgccgc gttcaccctc ctccaatgct tcatctcgat
     2761 caggcaagga gacctcctgc gcaagctgga ggaggaattg cctccagtat tgatacaatt
     2821 tcacctcgtg cacagatata cttaatgatt gacaaacaac agagtatctc ctggcaccgc
     2881 gaccggtcag caactatctc tgagatcatc ggcaccctcg cataccatgc caggattctg
     2941 tacaacgttg ggctgtacga tttctgtcag caccatgcga cgcacaggga gaacccgtga
     3001 tatcgttcag ggaatccgtc cgaatttgac cttgccggtt gacactgccg ctgggggaac
     3061 cagaacttcg tgcaggtccc ctatcacaag ttccggcaga agatgaagag caggtgcgaa
     3121 ctgtacgggc tctggttcga tgatacgcac ccgaaagccg atacgtcgca ggtcgatgct
     3181 tttgcactcg atccaatccg caagccctcc tatgggaaga cccggtgaat ccagagggga
     3241 ttgcaccaga gtgcccggga aaccccgatc aatgccggcg ggaatggcac tctgaaccat
     3301 gcaagaagag cagccggtga ttccgtggtt tcacggatga ccggtagtag gggccgcgct
     3361 aaccggccag cgtgaagaag gacgtttttt gacccgtcaa cctttgcaaa taataaattt
     3421 ctaccctgtg ccccgcaagg ggttcccatt gcaagccccc tccagtaggg gggtggttga
     3481 cttccagctc cggctgatag caccatgtac ctgacagatc cccatttttc agagacgttt
     3541 tcatgtatat gcgtaaaaaa acgttagttt tctgaaaagg ctgttagtat aggccttcat
     3601 gaagatccga cctgaagttc cctttttaca gcaccgccat gtacgggtcc acgcacaagc
     3661 tttatcatac acctggacaa agtagtttca ctttactccg atcgggacgc tggataggtc
     3721 catgcccgca gacagtaatc cgacggccga aagaacctgc aacaacgatg tgatatcatg
     3781 actcacagaa aatgggacgc cccaaggtta ttcccgctgc gcgagggtac cctggcggtc
     3841 agtcatgggg atacgtttg
//
LOCUS       DAOQVJ010000095         3853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26695_length_3853_cov_3.800948, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000095 DAOQVJ010000000
VERSION     DAOQVJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26695_length_3853_cov_3.800948"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKL26_02955"
     CDS             complement(<1..704)
                     /locus_tag="PKL26_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HNS82686.1"
                     /translation="MDEITVRYLGIEPGEVSAPILIEGLPGIGHVGKLVAEHLIHELG
                     ARKIAEIESIFFPPQVIIGENGTVRLANNEIYFYDGEDRRILFLVGDFQSTSAEGHYL
                     LADTFLSIAEELGVSRTYTLGGYGVGHLVDVPRVLGAVNDGMLRDEIEAAGAFISPDE
                     PGGGIIGAAGLLLGLGRARGIEGICLMGETSGYLVDPKSASRVLGVLCTLLDLRVDAT
                     TLQERGMEMEHMIQKLV"
     gene            complement(692..853)
                     /locus_tag="PKL26_02960"
     CDS             complement(692..853)
                     /locus_tag="PKL26_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HNS82687.1"
                     /translation="MSGRLRRCPRDRTYTLQEQCPACGEPTVTAHPPRFSPEDRYGHY
                     RRVMKGWTR"
     gene            complement(850..1635)
                     /locus_tag="PKL26_02965"
     CDS             complement(850..1635)
                     /locus_tag="PKL26_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HNS82688.1"
                     /translation="MSEREWPEQAELVVCTVKDVKDFAAFVSLDEYPGRVGLIPISEV
                     ATGWIKYIRDHIREGQKVVCKVLHVDTQRGHIDLSLKDVNEHQRREKIREWKNESKAR
                     KWIGFAAQAAGVKAEEIEASIYSHYGDLYSVFEDIVSGEHSLDSLGISPGAASALQQV
                     ARENVKMPRVTVGGNIILTSTKPDGVNIIRRALRSAEPKIPDVDIELSYLGAPAYRIK
                     VTARDYKTAEKAIEKAAAAAIGVVERAGGEGKFIKKPKSGKTA"
     gene            complement(1644..1832)
                     /locus_tag="PKL26_02970"
     CDS             complement(1644..1832)
                     /locus_tag="PKL26_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HNS82689.1"
                     /translation="MVRQLRENRSKFFRVKCADCENEQVVFEKASTTVECVVCGKVLA
                     EPSGGKAQLKAEVLATLE"
     gene            complement(1838..2116)
                     /locus_tag="PKL26_02975"
     CDS             complement(1838..2116)
                     /locus_tag="PKL26_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HNS82690.1"
                     /translation="MKMPVKFKTYCPFCRSHQVHEVEKVKKSRTSGLHWIDRQKARRS
                     TVGSMGKFSKVPGGDKPTKKINVRYRCSTCKKAHLRGGFRAGKFELTE"
     gene            complement(2154..2780)
                     /locus_tag="PKL26_02980"
     CDS             complement(2154..2780)
                     /locus_tag="PKL26_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82691.1"
                     /translation="MHPVHLDELRILLLSERESGRLVQIQKDLFSSVGAGLETHYSSL
                     YACEDPFSDEARVLIERIAAIRETLLDLYRIRAEKILSLARSQEDGQYVDREELKKLL
                     PEEKDMFDQVVLALEQARCRLVDGSVPRAPSHLGSEREADRCASVLAAAAERPAFVVS
                     RVLQDMEPFMGVDGHIYHLQREDLVTIPERNAEVLCERNIVLNINPGK"
     gene            complement(2758..>3853)
                     /gene="priS"
                     /locus_tag="PKL26_02985"
     CDS             complement(2758..>3853)
                     /gene="priS"
                     /locus_tag="PKL26_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HNS82692.1"
                     /translation="PSSLPQREWAFVFFDPDFPDIRMRRHLGFETRDEAFSYIRSMAP
                     AHIYYSTAYYARPGAGTMQEKEWLGADLIFDLDADHIMRGPYDRMLSRVKEETKKLLD
                     MLVLELGFSPREIDLVFSGGRGYHVHVHDLSVRNWGSSERRELIDYVCGIGLDPGRVL
                     AHRTHSPRGWHHRYIAALAEYLLWLESLGEKERVAAIAGLEGVGKTTAEEFSRRLGEV
                     LSRITTDPASLNLKDAVIAKVIRGLSVQKEGQFAAMLRDRAALADEPVTTDTKRLIRL
                     PSSLHGGSGLRVTPLAPKDLDDFDPLVDAVVFGEREVKVDLAFPLSMPILGTSFRLEK
                     GISAVPEALAVFLCCRGAAEIAGGASSAPG"
BASE COUNT          668 a         1260 c         1171 g          754 t
ORIGIN      
        1 acgagcttct ggatcatgtg ctccatctcc attcccctct cctggagggt ggtggcgtcg
       61 acccgaaggt cgaggagggt gcagaggacg ccgaggaccc gtgatgcgct cttcgggtcg
      121 accaggtagc ccgaggtctc gcccatcagg cagatcccct cgataccccg cgcccgcccg
      181 aggccgagca ggagccccgc ggccccgatg atcccgccgc ccggctcatc cgggctgatg
      241 aacgcacctg cggcctcgat ctcgtcgcga agcatgccat cgttgaccgc cccgagcacc
      301 cgcgggacgt ccaccaggtg cccgacgccg tagccgccaa gggtgtaggt gcgggagacc
      361 ccaagctcct ccgcgatcga gaggaaggtg tccgcaagaa ggtagtgtcc ctctgcagac
      421 gtgctctgga agtcgccgac caaaaagagg atccgccggt cctcgccgtc ataaaaatag
      481 atctcgttgt tcgcgagccg gaccgttccg ttctccccga tgatcacctg cggcgggaag
      541 aagatcgatt cgatctccgc gatcttcctt gcgccaagct cgtggatcaa gtgctcggcc
      601 accagtttcc cgacgtgccc gatgccgggg agcccctcga tgaggatggg agcagagacc
      661 tccccgggct cgattccgag gtagcggacg gttatctcgt ccatcccttc atcaccctcc
      721 ggtaatggcc atagcggtcc tccggtgaga aacggggggg gtgggcggtc acggtgggct
      781 caccgcaggc agggcactgc tcctgcaggg tataggtccg gtcacgggga caccgcctca
      841 accgtccgct catgcagtct tcccggactt gggtttcttg atgaattttc cctcgccccc
      901 ggcgcgctcc acgacgccga tggccgcggc tgcagccttc tcgattgcct tctccgccgt
      961 cttgtaatcg cgggcagtga ccttgatgcg gtaggccggg gccccaaggt aagagagctc
     1021 gatgtccacg tcagggatct ttggctcggc gctccggagc gccctcctga tgatgttcac
     1081 cccgtcgggt ttcgtggagg tgagaatgat atttcctccc acggtgaccc ggggcatctt
     1141 cacgttctcg cgtgcgacct gctggagggc cgaggccgca ccgggcgaga tgccgagcga
     1201 gtcaagggag tgctcgccgc tgacgatatc ctcgaacacc gagtagaggt cgccgtaatg
     1261 ggagtagatc gacgcctcga tctcctcggc ctttactcct gcggcctgcg cggcaaaccc
     1321 gatccatttc cgggccttgg actcgttctt ccactcccgg atcttctccc tgcgctggtg
     1381 ctcgttcacg tcctttaagg agaggtcgat gtggcccctc tgggtatcca cgtggaggac
     1441 cttgcagacc accttctgcc cctcgcggat gtggtcgcgg atgtacttga tccaccccgt
     1501 cgccacctcg gagatgggga tcaggcccac cctgccggga tactcgtcga ggctgacgaa
     1561 cgcggcgaag tcttttacgt cctttaccgt gcagaccacc agttccgcct gttcgggcca
     1621 ctctcgctcg ctcatcggac caatcactcg agcgtcgcca ggacttcagc cttgagctgt
     1681 gcctttccgc cggatggctc ggcaaggacc ttgccgcaga ccacgcactc gactgtcgtg
     1741 ctggccttct cgaagaccac ctgctcgttc tcgcagtccg cgcacttgac cctgaagaat
     1801 ttgctgcggt tttccctgag ttgtcgtacc atcttcctca ctccgtcaat tcgaacttgc
     1861 ctgcccggaa cccgccgcga aggtgtgcct tcttgcaggt gctgcaccgg tagcgcacgt
     1921 tgatcttctt ggtgggcttg tcccctccgg gaaccttgga gaacttcccc atgcttccca
     1981 ctgtgctcct cctcgccttc tgcctgtcga tccagtgcag tccggaggtc ctgctcttct
     2041 tgaccttctc cacctcatgg acctggtgtg agcggcagaa tgggcagtat gtcttgaatt
     2101 tgactggcat tttcatagat atggcctcgt attcaggata tcgaatatca gtattatttg
     2161 cccggattga tatttaagac tatgttgcgc tcacagagga cctcggcgtt ccgctcggga
     2221 atggtcacca ggtcctcgcg ctgcaggtgg taaatgtgcc cgtccacgcc catgaacggc
     2281 tccatgtcct gcaggacccg tgagaccacg aaggcaggcc tctcggcagc ggcggcaagg
     2341 accgatgcgc agcggtccgc ctccctctcg ctccccaggt gggagggcgc ccgcggaacc
     2401 gacccgtcaa ccagccggca gcgggcctgc tcgagggcaa ggaccacctg gtcaaacatg
     2461 tccttctctt ctgggaggag cttcttcagc tcctcccggt cgacatactg cccgtcctcc
     2521 tgggagcgtg cgagggagag gatcttctcg gcccggatgc gatagaggtc gagcagcgtc
     2581 tcgcgtatcg cggcgatccg ctcgatgagg acccgcgcct cgtcggagaa cgggtcctcg
     2641 cacgcgtaga ggctggaata atgcgtctcc aggcctgctc cgaccgagga aaagaggtct
     2701 ttctggatct gcacgagccg gcccgactcg cgctccgaga gcaggaggat ccgcagttca
     2761 tccaggtgca ctggatgcac ctcctgcgat ctccgccgca cccctgcagc agagaaagac
     2821 cgcgagcgcc tcggggaccg cggagatccc tttctccagc cggaaagagg tgccgaggat
     2881 cggcatcgag agcggaaagg cgagatcgac cttcacctcg cgctcgccaa agacgacggc
     2941 atcgacgagg ggatcgaagt cgtcaaggtc ctttggcgca agcggggtca cccggagacc
     3001 actcccgcca tggagcgagg acgggagccg gatcaggcgc ttcgtgtcgg tcgtgaccgg
     3061 ctcgtccgcg agcgcggccc tgtcccggag catggcggca aactggccct ccttctgtac
     3121 ggagagcccc cggatcacct ttgcaatgac cgcgtccttc aggttgaggg acgcagggtc
     3181 agtggtgatg cgggagagga cctcgccaag gcgccgcgaa aactcctcag cagtcgtctt
     3241 ccccaccccc tcgaggccgg caattgcggc caccctctcc ttctccccga ggctctccag
     3301 ccataggagg tactcggcaa gtgcagctat atagcgatga tgccatccgc ggggtgaatg
     3361 tgtgcggtgg gcgaggaccc ggcccgggtc aaggcctatc ccgcacacgt agtcgatcag
     3421 ctcccgccgc tcgctgctgc cccagttccg gaccgagagg tcgtggacgt ggacatggta
     3481 gccccgtcct cccgaaaaga cgagatcgat ctcgcggggc gaaaacccaa gttccagtac
     3541 cagcatgtcg aggagtttct ttgtctcctc cttgacccgc gagagcatcc tgtcgtaagg
     3601 cccgcgcatg atgtggtcgg cgtcaagatc gaagataagg tcggccccga gccactcctt
     3661 ctcctgcatg gtccccgccc ccggcctggc atagtacgca gtagagtagt aaatgtgcgc
     3721 gggggccatg ctcctgatgt aggagaaggc ctcgtcccgc gtctcaaagc caaggtgcct
     3781 gcgcatccgg atgtcgggaa aatcggggtc gaagaagacg aaggcccact ccctctgggg
     3841 aagggacgat gga
//
LOCUS       DAOQVJ010000096         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26752_length_3847_cov_3.722310, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000096 DAOQVJ010000000
VERSION     DAOQVJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26752_length_3847_cov_3.722310"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKL26_02990"
     CDS             complement(<1..227)
                     /locus_tag="PKL26_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82693.1"
                     /translation="MKHPLIILTVLLAGMMVCSQFCSAWITYESNASRGIPSELVEKN
                     KAIQEKYKEIAESGLPQFIKPTNPFPLPSTTV"
     gene            complement(224..>502)
                     /locus_tag="PKL26_02995"
     CDS             complement(224..>502)
                     /locus_tag="PKL26_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNS82694.1"
                     /translation="PRCGCISFPESYRSLTRISRPHALITERDGRFVLEDRGSTGGTY
                     LNGKRIAERQQVLLRDGDRISLSLGEFGATLLFLHKPDQDTGEPGAGM"
     assembly_gap    503..512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<513..1045)
                     /locus_tag="PKL26_03000"
     CDS             complement(<513..1045)
                     /locus_tag="PKL26_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNS82695.1"
                     /translation="MAEGQGTILTADDAEFLEELSEYLEILGNNTRLRILRLLEKKPL
                     DARSVSIATGISYENTKKHLERLLHAGLITRSPGLGRETSRGVHPVWEYSPVPDVIRR
                     VQRDLGFLFALPPHAGWSGLHGQVSTVRQRLSLEWGTLEKLLLVMGGEWDGRTLPLAS
                     PCTALGRVEPGVDASLPR"
     gene            complement(1153..1653)
                     /gene="msrB"
                     /locus_tag="PKL26_03005"
     CDS             complement(1153..1653)
                     /gene="msrB"
                     /locus_tag="PKL26_03005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNS82696.1"
                     /translation="MKDPSKDPSRICIFSLSTGKLELVDRVVLSQEEWQRRLSAGQYR
                     VARMKGTEPAFSGEYHDLHDVGLYRCICCGTDLFASYSKFDSGTGWPSFSAPVHMNNV
                     GFHEDRSFGMVRTEVTCARCDAHLGHVFDDGPPPARMRYCINSASLSFVPVHGKNREK
                     GDMDSR"
     gene            1769..3025
                     /locus_tag="PKL26_03010"
     CDS             1769..3025
                     /locus_tag="PKL26_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNS82697.1"
                     /translation="MEIAERKESGVVVLVCRGRLDGHGAMVLEQAAKEALHDDDRSLV
                     LDMKNVPYLSSAGIRVMLVLQKRLRDRGGKVALAGTGDFPRKVLEMAGFLSIFPDYPT
                     AEEAVRVLGKAEERASLLADLQSPSFEVNGARVRIEQGSTRPSALRLWGDMDVVLNSR
                     IDAPHLAVIPFSGIEYALGLGALGKSPEDVLPFLGEMVALHGSLVWLPADGNDTPDFF
                     TPVKDTGEVRIFSGYSVSLEGPFREFVILESIPREGMRLGEIYRLLIERAKKEHLEFR
                     GVLAVAWWAVLEGLQSQDIPTRNPSVQDSRFAQGAIPRYRGDTLVGFGIAVDLDLAEK
                     HFDRAVLNSFFSPHGEENEGGPRMITHTHGVVFRNVAYDPGASLEGQMKKILGQGEFV
                     DMRHLLDETRIRKAKIGIAPVSSLIT"
     gene            complement(3160..>3847)
                     /locus_tag="PKL26_03015"
     CDS             complement(3160..>3847)
                     /locus_tag="PKL26_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNS82698.1"
                     /translation="DLPVSVSGTPYQTYTFKKEGYYTYNGNINTVPAKGQVFHIYVSM
                     TAMPLVEYSRVHLLVSPPDAKVTWDGEPAGDVPPTGILIIYNVAPGKHTVKVTKEGFL
                     PIEQALTVTGNDVMRVPLSLTPVPLGSITFESVPAGASVYLDGQFRGMTPLSLHDVPA
                     GSHAVMITSEGFQDVVSAVEVTGGKTTLVSETLNPGTPVPGSTRAALSPMVLAGGLAI
                     AGLLVAYRRP"
BASE COUNT          795 a         1097 c         1159 g          786 t
ORIGIN      
        1 acagtcgttg agggcagcgg gaatggattg gtgggcttta tgaactgggg aagaccgctc
       61 tctgcaatct ccttgtactt ctcctggatt gccttgttct tctcaaccag ttccgacggg
      121 atgccccgtg aagcgttgct ctcgtaggtg atccatgccg agcagaattg gctgcatacc
      181 atcattcctg caaggagcac cgtgagaata atgagaggat gcttcacatc cctgcccccg
      241 gttcaccagt gtcctgatca ggcttatgca gaaagaggag agttgcgccg aactccccga
      301 gtgagagcga gatgcggtcg ccgtccctga ggagcacttg ttgacgctcc gcaatcctct
      361 tcccgttgag atatgtgcct ccggtactgc cgcggtcctc gagaacaaac ctcccatccc
      421 gctcggtgat cagtgcatgc ggcctcgaga tccgtgtcag cgacctgtaa gactcgggaa
      481 aagagatgca tccgcatcgc ggnnnnnnnn nncgcgggag gctcgcatcc acgccaggct
      541 ccactcttcc cagcgcagtg cagggggatg caagagggag tgtccggcca tcccattcgc
      601 cccccattac gaggagtaac ttctcgagcg tcccccattc gagggagaga cgttgcctga
      661 ccgtggacac ctgtccatgc aggccactcc acccggcatg agggggcagc gcgaagagga
      721 acccgaggtc gcgctggacg cgccggatga cgtcagggac aggagaatat tcccatacgg
      781 ggtggactcc gcgggatgtc tccctcccga gccccgggct cctggtgatc agacctgcgt
      841 gaagaagacg ctccaggtgt ttcttggtgt tctcgtagga gatccctgtg gcaatcgaga
      901 ccgagcgtgc atcgagcggc tttttctcaa ggagcctgag gatacggagg cgcgtgttgt
      961 ttccaaggat ctcaaggtat tccgagagct cctccaggaa ctccgcatca tcggcggtca
     1021 gtatggtgcc ctgcccctct gccatgcatc tttcctcccg ggaccattcc tgcaggagag
     1081 gtatgcggga tgtgttcaag agagtgccgg ttggacggcc ccggacccgc ctgccaccgg
     1141 aactctcttt tgtcaacggc tgtccatgtc tcccttttcc cggtttttcc cgtgaaccgg
     1201 gacaaatgag agggatgccg aattaatgca gtatctcatt ctcgcaggcg gaggaccgtc
     1261 gtcgaagacg tggccgaggt gcgcatcgca acgggcgcat gtgacctctg tcctcaccat
     1321 cccgaaactc cggtcctcat gaaaaccgac attgttcata tgcaccggtg cactgaaact
     1381 cggccatcct gtaccggaat caaactttga gtatgaagcg aagaggtctg tcccgcagca
     1441 gatgcatcgg tagagcccaa catcatgaag gtcatggtat tctcccgaga aggcaggctc
     1501 tgtccctttc atcctggcaa cgcggtattg cccggccgat aacctcctct gccactcctc
     1561 ttgggacagg acaaccctgt ccaccagttc cagcttcccg gtcgagaggg agaagataca
     1621 gatgcgggac ggatccttcg aggggtcttt catgctgcac tcactctttt cttcagctgg
     1681 gcataaagaa ggagtttgtg gcagtggaaa aaccacgaat gctatcatgt tgcccgcgaa
     1741 acctctggag cgtatcggtg atgcccctat ggagatagca gagaggaagg agagcggggt
     1801 ggtggtgctg gtgtgccgcg gaagattgga cgggcacggc gcgatggtcc tggaacaggc
     1861 cgcaaaagaa gccctgcacg acgacgaccg ttcacttgtg ctcgacatga agaatgtccc
     1921 ctacctttcc agcgccggga tcagggtcat gcttgtcctg caaaagaggc tgcgggatcg
     1981 cggcgggaag gtggctctcg cgggaacggg tgattttccc aggaaagtcc ttgaaatggc
     2041 aggttttctc tccatcttcc cggattatcc aaccgcagaa gaggccgttc gcgtcctcgg
     2101 gaaggcggag gagcgtgcat ccctgcttgc cgatctgcag tccccgtcct ttgaagtgaa
     2161 cggggccagg gtacgtatcg agcagggttc cactcggccg tcggctctcc ggctatgggg
     2221 tgacatggat gtggtgctga attcccgcat cgacgcaccg cacctcgcgg tgataccctt
     2281 cagcgggatc gaatatgccc tgggcctggg ggctctcgga aaaagtcccg aggatgtcct
     2341 cccgttcctt ggagagatgg tggcgctcca cggctcgctg gtctggctgc cggcggacgg
     2401 caacgacacg cctgacttct tcactccggt gaaggacacc ggggaggtca ggatcttctc
     2461 cgggtactca gtatccctgg agggtccttt ccgggagttt gtcatcctcg agagtatccc
     2521 gcgcgaggga atgcgccttg gagaaatcta ccgcctcctc atcgagaggg cgaagaaaga
     2581 gcacctcgag ttcaggggag tcctggcagt ggcgtggtgg gcagtcctcg aaggactgca
     2641 gagccaggat atccccacca ggaacccttc ggtgcaggac tcacggttcg cgcaaggagc
     2701 gatccccagg tacaggggtg ataccctggt gggttttgga atcgcggtcg acctcgatct
     2761 cgcggagaag cacttcgaca gggcagtcct gaactcattc ttctccccgc atggggagga
     2821 gaatgaggga gggccgcgga tgatcacaca tactcatgga gtggtattca ggaacgtcgc
     2881 atacgaccct ggcgcatcgc tggaagggca gatgaagaag atacttggcc agggggagtt
     2941 cgtggacatg cgccacctcc ttgacgagac aaggatccgc aaggccaaga tcggcatcgc
     3001 tcctgtcagc tcgctcatca cgtgaaatct caacgccacc tgcagccggc agggagaata
     3061 atccgcgggg tctccctcac aagaaacatt gctgcattca ctgaggagaa cccgccagga
     3121 gaatacaggc agaacaggca ggaaaaaagt aacaggaagt cagggccttc tgtatgccac
     3181 gagcagccct gcaatcgcca ggccccccgc cagcaccatt ggcgacaacg cggccctcgt
     3241 gcttccggga accggcgtcc cagggttgag cgtctcgctc accagggtgg tctttcctcc
     3301 tgtgacctcg actgcggaga ccacgtcctg gaacccttca ctggtaatca tgacggcgtg
     3361 gcttcccgca ggaacatcat gcagtgaaag cggagtcatt cccctgaact ggccgtcgag
     3421 atagacagac gcacccgccg ggacggattc aaaagtgatg gaaccgaggg gcacaggcgt
     3481 aagtgacagc ggcaccctca ttacatcgtt cccggtgacc gtcagggcct gctcgatcgg
     3541 gaggaacccc tctttggtaa ccttcacggt atgcttgccg ggcgcgacat tgtatatgat
     3601 caggattcct gtcggtggca catctcctgc cggttcaccg tcccaggtaa cctttgcatc
     3661 gggagggctg accagcaggt gcaccctgct gtattcaacg agtggcatcg cggtcatcga
     3721 tacgtaaatg tggaagacct ggccttttgc gggcacggta ttgatgttgc cattgtaggt
     3781 gtaatagccc tctttcttga aggtatacgt ttggtaaggg gttcccgaaa cagagacagg
     3841 gaggtcg
//
LOCUS       DAOQVJ010000097         3845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26776_length_3845_cov_4.285752, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000097 DAOQVJ010000000
VERSION     DAOQVJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26776_length_3845_cov_4.285752"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKL26_03020"
     CDS             complement(<1..447)
                     /locus_tag="PKL26_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HNS82699.1"
                     /translation="MTRVRIAQLSCGPEYSGVQKEIEAAAASVDAEVFFPDLKLADIR
                     HSFNEFGLEVRSPDLKLAIARAKALVEGRVEADAVFIATCFRCAEAAIVRNELRRYIH
                     ENSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREVQQGLT"
     gene            complement(444..893)
                     /locus_tag="PKL26_03025"
     CDS             complement(444..893)
                     /locus_tag="PKL26_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HNS82700.1"
                     /translation="MAKVFLYPTTSLILSDLVARFGHKPLSAALKIRERIQTPGIDSP
                     PLQITPEEPKKGLKWAAVEVPSGVRGRMSLYGPMIDEAEAAIIINNADYSFGCMGCAR
                     TNELIKYLIRKRDIPVLDLEYPTTEDEGVEFVAAIKKFLDTLGEGSP"
     gene            complement(895..1353)
                     /locus_tag="PKL26_03030"
     CDS             complement(895..1353)
                     /locus_tag="PKL26_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HNS82701.1"
                     /translation="MTAYKPRYAGSVTKYVFVESPEMTPSDLAIRAYEISQGVMIKET
                     CFGLQVTGKEEDVERIIASLRAFDPTHIFVKDRGFPPGDPRRCRANLGGDRPGYFGHE
                     FEMGLIRNISRGLALLPSRAQDDLPHPPAPSKEPRLDASRLKKIIESQES"
     gene            complement(1350..2879)
                     /locus_tag="PKL26_03035"
     CDS             complement(1350..2879)
                     /locus_tag="PKL26_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HNS82702.1"
                     /translation="MINILLDGEKLETGDGARLRDLLPDHDKACCVGIIRPGARESAT
                     TGSMRLDTSAGEVVVELNPAGQAIFGSASLQFPLSLHWQDRYAAAFGPFHSSFTPART
                     ASLYERGDVVLGCGGYDPHRSYLIFCRSRHTSDHGAAAGGGVIGKVVSGRGVLDRWST
                     GDLVNTASPVLNWADTSRSFTTTDPDTVLEDGMEVISHLRITSFGYSGSEVTSTAAAE
                     SIEHLLLALEDAHFVVGRSSSTHIRDDRKAGTEVAYQVKQPRREGAVSVRTAGRSGGS
                     VYIYIQDLPGTPQHTVVGQVTHGLELARLARDGDVLSARVVPSRFDLIGLPLEQAIDT
                     AEQRGIRILGELEGRDLVVVGQEPATTIESLAAGAVELSVVPLEKVIDITLDDDAAPA
                     SCDIFRHLTGLHLHDVGRIPFFFNFEDVFLFKPKIPRGVNIIPENTPTDLVPSGALAI
                     TNDSRKGSGLVGVRTSENSEFGPTSEPFEGTNIIGKVLDLEKLAALQEKEIVYIRERQ
                     P"
     gene            complement(2890..>3845)
                     /locus_tag="PKL26_03040"
     CDS             complement(2890..>3845)
                     /locus_tag="PKL26_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82703.1"
                     /translation="TNHMGMVVTSHFSQVINEVADRAMLLAEGKIAALGSPEEVIRTF
                     MADLSDTEEFEVPSFGGEIMIARDVFKRYISVDRGVVRAVNGVTFEVKEKEIFGIIGK
                     SGAGKTTLSRIISGIIEPTTGEMIIRIGDDSVDMTKPGIENRGRAKGYIGLLHQEYDL
                     YPHRTVLDNLTDAIGLEFPKELAMRKAIITLKMAGFTEAKSREILDRLPGQLSEGERH
                     RVALAQVLIREPRMVILDEPTGTMDPLTKIDVKHSIMHARDEMDETFIVVSHDMDFVR
                     DICDRLALMRGGKIVQIGQTAEVLASLTEEEREVMGKAVTS"
BASE COUNT          685 a         1183 c         1142 g          835 t
ORIGIN      
        1 ggtgagcccc tgctgaactt cacgtgcaag gagcgccctt ctccgggcga tagtggtcag
       61 ggcctccatg cgggtgagga gtgtccccgc ggtggtacgc tcggtgaacg agtaactgac
      121 caccgggagc cgggagttct catggatgta gcggcgaagc tcgttcctga cgattgctgc
      181 ctcggcgcac ctgaaacagg ttgcaatgaa gactgcgtct gcctcgaccc tcccctccac
      241 gagcgccttg gcgcgggcta tcgcgagctt gaggtcaggg cttcgcacct cgagaccgaa
      301 ctcattgaac gaatgcctga tgtccgcaag tttcaggtcg ggaaagaaga cctctgcatc
      361 caccgaagcc gcagccgcct cgatctcctt ctgcaccccg ctgtactcgg ggccgcagga
      421 gagctgcgca atcctcaccc tggtcatggt gaaccctccc caagcgtatc caggaatttc
      481 tttattgcgg ctacgaactc gacaccctca tcctctgtcg ttggatattc gagatcgagg
      541 accgggatgt cccgtttccg gataaggtac ttgatcaact cgtttgtcct ggcacatccc
      601 atgcacccga aagagtagtc tgcgttgttg atgatgattg ccgcctccgc ctcgtcgatc
      661 atggggccgt agagggacat cctcccccga actcctgacg ggacctccac ggcggcccac
      721 ttgagaccct tcttcggctc ttcaggggtg atctggagag ggggggagtc gatcccgggt
      781 gtctggatcc tctctcggat cttgagtgct gcagagaggg gtttgtgccc gaaccgtgcc
      841 accaggtctg agaggatgag gctcgtggtg ggatagagaa agaccttcgc catgtcagga
      901 ctcctgtgat tctatgatct tcttcaaccg ggaggcatcg agcctcggtt ctttcgaggg
      961 ggcaggggga tgcgggaggt catcctgtgc ccgtgagggg agcaacgcaa ggcctctgga
     1021 gatgtttcgg atcagcccca tctcgaactc gtggccgaag tatcccggcc tgtctccccc
     1081 gaggttggcc cggcacctcc tggggtcccc gggggggaac cccctgtctt ttacgaagat
     1141 gtgggtcggg tcgaatgctc ggagcgatgc gatgatgcgt tccacatcct cctctttccc
     1201 tgtgacctgg agtccaaaac acgtctcctt gatcatcacc ccctgcgaga tctcgtatgc
     1261 cctgatcgcg aggtcggagg gcgtcatctc cggcgactca acgaagacat acttggtcac
     1321 ggaacctgcg taccggggct tgtaggccgt catggttgtc tctccctaat gtatacgatc
     1381 tctttctcct gcagcgcagc aagcttttcg aggtcaagga cctttccgat gatgttcgtc
     1441 ccttcgaaag gctctgaagt cggtccgaac tcgctgttct cactggtgcg aactcccaca
     1501 agccccgacc ctttcctcga gtcgttggtg atggcaaggg cccctgaggg gaccaggtca
     1561 gtgggggtat tttccggtat gatgttcaca cccctcggga ttttcggctt gaagaggaac
     1621 acgtcctcga aattgaagaa gaacgggatc ctccccacat cgtggaggtg aagtcccgtg
     1681 aggtgcctga agatgtcgca ggacgcgggg gcagcatcat cgtcgagcgt gatgtcgatg
     1741 accttctcga gcggcaccac cgaaagttcc acggcacctg ccgcaaggct ctcgatcgtc
     1801 gtggcaggct cctgcccgac gacaacgagg tcgcggcctt ccagttcccc caggatccgg
     1861 atgcccctct gttcagcggt gtcaattgct tgctcgagag gaagcccgat gagatcaaag
     1921 cgggagggca cgaccctggc ggagagcacg tcgccgtccc gggcaagtct tgccagttcg
     1981 agcccgtgcg tgacctgccc taccacggta tgctgggggg tcccgggcag gtcctggata
     2041 tatatgtata ccgaaccccc ggacctcccc gccgtcctca cgctgaccgc tccctcgcga
     2101 cgaggctgct tcacctggta ggctacctcg gtccccgctt tcctgtcatc cctgatatgg
     2161 gtgcttgacg accgtcctac cacgaaatgt gcatcttcga gtgcgaggag gaggtgctcg
     2221 atgctttcgg ctgcggccgt ggaagtcact tcactgccgc tgtatccaaa cgacgtgatc
     2281 cgcagatgcg agatcacttc catgccgtcc tcgaggacgg tgtccgggtc cgtggtggtg
     2341 aaagaccggc tggtgtcggc ccagttgagg acgggagatg ccgtgttgac gaggtcgccc
     2401 gtgctccacc ggtcaaggac gccccgcccg ctgaccacct ttccgatcac gccgcctcct
     2461 gcagcggcac cgtggtccga ggtatggcgg ctccggcaga agatgaggta ggacctatga
     2521 gggtcatacc ctccgcagcc gaggaccacg tcccccctct cgtacagcga cgcggtcctg
     2581 gccggggtga acgacgaatg aaacgggccg aacgcggcgg cgtacctgtc ctgccagtgc
     2641 agggagaggg gaaattgcag gctggcggat ccaaatatcg cctgccccgc aggattcagc
     2701 tcgacgacca cctcgcctgc agaagtatcg agccgcatgc tcccggtcgt ggcggattcg
     2761 cgggcgcctg gacggatgat tccgacgcag cacgccttgt catggtcagg cagcaggtct
     2821 cttaaccttg cgccatcccc ggtctccagc ttctctccgt cgagcaggat gttgatcatg
     2881 cctgtccctt caggaggtga cagccttgcc catgacctct cgctcttctt cagtaaggct
     2941 cgcgaggacc tcggcggtct gtccgatctg cacgatcttt cctcccctca tcaatgcaag
     3001 gcggtcgcag atgtcccgca caaagtccat gtcatgggag acgacgatga acgtctcgtc
     3061 catctcgtcg cgggcgtgca tgatggagtg cttgacgtcg atcttggtga gggggtccat
     3121 cgtcccggtc ggctcgtcga ggatcaccat cctcggctca cgtatcagca cctgtgcgag
     3181 tgccacgcgg tgccgctccc cttccgagag ctgcccgggg aggcgatcaa ggatctccct
     3241 gctctttgcc tcggtgaacc ctgccatctt gagggtgatg atggctttcc tcattgccag
     3301 ttccttgggg aactcgagac ctatggcatc cgtgaggttg tcaagcaccg tgcggtgggg
     3361 ataaaggtcg tactcctggt gcaggagacc gatatagccc tttgccctcc ccctgttctc
     3421 gatcccgggc tttgtcatat cgaccgaatc gtcaccgata cggatgatca tctcgccggt
     3481 tgtcggctcg ataatcccgg agatgatccg tgagagggtg gtctttcctg ctccgctctt
     3541 cccgatgatc ccgaatatct ccttctcttt tacctcgaac gtgacaccgt tgaccgcccg
     3601 tacgactccc ctgtccactg agatatagcg cttgaagaca tcccgtgcga tcatgatctc
     3661 tcctccaaag gaggggacct cgaactcctc ggtatcggag aggtcagcca tgaaggtacg
     3721 gatcacctct tcgggggacc cgagtgcggc gatctttccc tctgcaagga gcatagccct
     3781 gtcggcaact tcgttgatca cctgggagaa gtgggaggtg acgaccatgc ccatgtggtt
     3841 cgtct
//
LOCUS       DAOQVJ010000098         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_26865_length_3836_cov_4.199154, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000098 DAOQVJ010000000
VERSION     DAOQVJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26865_length_3836_cov_4.199154"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /gene="sppA"
                     /locus_tag="PKL26_03045"
     CDS             <1..637
                     /gene="sppA"
                     /locus_tag="PKL26_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNS82704.1"
                     /translation="SVVRVEGMIVTGNSHGDGFVGSEIVGRMIRDAADDPIVDAIVIR
                     INSPGGTPAAAQEIAADIRYARNKKPVVVSMGDIATSAAYYVSAYADRIYANPDTLTG
                     GIGTSWSFLDISGWLEEKNISVVVFKSGSMKDMGSDHRPLTPDEREYAQMVVNQSNER
                     LLTGIIAERGINQSDIEDARVIRGEQALLLGLVDEIGNLNDAIAGARRLAG"
     gene            complement(679..1947)
                     /gene="truD"
                     /locus_tag="PKL26_03050"
     CDS             complement(679..1947)
                     /gene="truD"
                     /locus_tag="PKL26_03050"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNS82705.1"
                     /translation="MRETPYPLEQSLGMRWYASNTPGIGGRLRTTAEDFVVEETSSGP
                     GDEGPYLICRLTKKDWELQRAVREIAHALSISHRRIGWTGTKDKHAVTTQLISIYGVS
                     EDDLGRIRLKDLEVVPVGRSREPLLLGAHDSNHFTITIRDTTSPDLGSEVAEVAAACG
                     RGIPNYFGIQRFGVIRPITHLVGEHILKGDFDLAVDCYVGMACPGEPPAVREAREAYA
                     EEKDTLAALRSLPVPLTYERAMLHHLVSHPGDFRGALLSLPPKLLSMFVSAFQSYLFN
                     MALSARMERDPDFSLPAPGDRLIFSNGREDTVTVANRDAAMMQVARGRCKVAIMMPGS
                     RAVRETGEDDRAVAALLSMHDIVPDSFSRASSFVKARYDGALRPVPLTAEVMSTVTGP
                     DVTLSFSLAPGQYATTVCREFMKADPRAMV"
     gene            complement(1994..2356)
                     /gene="pth2"
                     /locus_tag="PKL26_03055"
     CDS             complement(1994..2356)
                     /gene="pth2"
                     /locus_tag="PKL26_03055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HNS82706.1"
                     /translation="MEKEPRFEWKQCLVVRSDIRMSCGKTCAQVAHAAVIAYDRTDAS
                     TRKKWLQEGQKKVVLKVPSERALLELKTLAEYAGISTGLVQDAGLTEIPPGTVTALGL
                     GPAKSEDLDRITSSLSLF"
     gene            2700..3809
                     /locus_tag="PKL26_03060"
     CDS             2700..3809
                     /locus_tag="PKL26_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS82707.1"
                     /translation="MTRQYDVVVVGGGPAGSTCGRLCAEQGLSVCLVEEHGAAGIPVQ
                     CAGLLSMNAFRECEVSQSSILATVRGAKVVSSLGPVLEFDARMPKAHVVDRAVLDREM
                     LARAAGAGAEVRMKTSFADRKGQRIVTRGVNGKEEILFRILVAADGARSRVARVMGMR
                     RPEVFLAGLQADILHGMDGSRVEIHPDAAPDFFGWAIPIGAGRARVGLAGRTGVRERF
                     RSFLSLISQDSHQAQVHLVSGTIPLGTMARTFGDRVLFVGDAAGMAKPTSGGGVYTGV
                     RAARHAARVAGEAVALEDFGDGMLSRYERAWKAIPSSRWFWIQLLPRNWRPWFRRFWP
                     AIKRSCSAANWPMPTPRMRRIRCCIWQRSGIRFWT"
BASE COUNT          746 a         1089 c         1287 g          714 t
ORIGIN      
        1 ctccgtcgtg agggtggagg ggatgatcgt gacagggaat agccacgggg acgggttcgt
       61 gggttccgag atcgtgggcc gcatgatcag ggatgccgca gacgacccga tcgttgatgc
      121 aatcgtgatc aggatcaaca gcccgggcgg aacccctgcc gcggcacagg agatcgcggc
      181 agacatccgc tatgcccgga ataagaaacc agtcgtggtc tccatgggcg atatcgccac
      241 ctcggccgca tactacgtga gcgcgtatgc ggaccggatt tacgcaaatc ccgacaccct
      301 caccgggggg atcgggacat cgtggtcgtt tctcgacata tcggggtggc ttgaagagaa
      361 gaacatcagc gtcgtcgtct tcaagtccgg cagcatgaag gacatgggct cggatcaccg
      421 ccccctcact cccgacgaga gggagtatgc gcagatggtg gtgaaccaga gcaacgagag
      481 gctcctcaca ggtatcattg cagagagggg gataaaccaa agcgatatcg aggatgcaag
      541 agtgatacgg ggcgaacagg ccctccttct cggactggtg gacgagatcg gcaacctcaa
      601 cgacgcgatc gcgggggcca ggcggcttgc ggggtaatat cctgggatac cagtaacgcc
      661 cccaccacta ccacagcatt agaccattgc cctgggatcg gctttcatga actccctgca
      721 caccgtggtg gcgtactgcc ccggcgcaag agagaaagag agggtcacat ccggacccgt
      781 gacggtgctc atgacctccg ccgtaagcgg gaccggccgg agggcaccgt cgtacctggc
      841 tttcacgaac gatgaggcac gggagaacga gtccgggacg atatcatgca tcgagaggag
      901 tgcggcaacg gcccggtcgt cctcccctgt ctcccgcaca gccctgctgc cgggcatcat
      961 gatggccacc ttgcagcgcc cccgtgcaac ctgcatcatg gcggcatccc ggttcgcgac
     1021 ggtgacggta tcttccctcc cgtttgaaaa gatcagccgg tcaccggggg cagggaggga
     1081 gaaatcaggg tcccgctcca tcctggcaga gagcgccatg ttgaagaggt aggactggaa
     1141 ggcgctcacg aacatcgaga ggagctttgg ggggagggag aggagggcac cccggaaatc
     1201 accggggtgc gagacgaggt gatggagcat tgccctttcg taggtgaggg ggacggggag
     1261 ggaccggagc gctgcaaggg tgtctttttc ttctgcatat gcttcccgtg cttcccgcac
     1321 cgccggaggc tcgcccggac aggccatgcc tacgtagcag tcaaccgcca gatcaaaatc
     1381 acctttcagg atgtgttctc ccacgaggtg ggtgatgggc cggatcacgc cgaagcgctg
     1441 gatcccgaag tagtttggaa tgccccggcc acatgcggca gccacctccg cgacttcgga
     1501 acccaggtcg ggggaagtgg tatcgcggat ggtaatggta aagtggttgg agtcgtgggc
     1561 gccgaggagg agcggttcac gggacctccc gaccggcacg acctcgaggt ccttcaaccg
     1621 gatccggccg aggtcatcct cgctgacccc gtaaatcgag atgagctggg tggtcaccgc
     1681 gtgcttgtcc ttggtcccgg tccacccgat ccgccggtgg gatatggaga gggcatgtgc
     1741 gatctcccgg accgcccgct ggagttccca gtctttcttg gtgagcctgc agatgaggta
     1801 tggcccctca tcgccgggcc cagaagaggt ctcctcaaca acaaaatcct ctgccgtggt
     1861 gcgaagcctt ccgccaatac caggagtgtt gctggcatac cagcgcatac cgagggactg
     1921 ttccaggggg tagggggtct ctctcatgtg ggattctcgc atctatacgg gtggacccgg
     1981 gcgcgttttg ccatcagaac agggaaagcg aactggtgat tcggtccagg tcctcgctct
     2041 ttgccgggcc aaggccgagt gcggtcacgg ttcccggcgg gatctcggtg agccccgcgt
     2101 cctggacaag gccggtggag atgccggcat actcggccag ggtctttaat tccaggagcg
     2161 cccgctctga aggcaccttg agcaccactt tcttctgacc ctcctgcagc catttctttc
     2221 gtgtcgaggc gtcggtccgg tcgtatgcga tgactgcagc atgggcaacc tgcgcacagg
     2281 tcttcccgca gctcatcctg atatcgcttc gcaccaccag gcattgtttc cattcaaacc
     2341 ggggctcttt ctccatctgt ggtgaaatat tcgcgggcag aggagaaaat aggtgtgaag
     2401 gggggaaacg ccgcaagggc tcctcgcctc gaccccgggg cgatatgtct gtcgccagga
     2461 ttcacagttt ttttcagcgg ggagggggag aatcccccca cccccccctc ctattcgcga
     2521 tagactgcat gtaaggtaca gagggcagat atgatctcct ttggatttcc cagcactctc
     2581 cactgcagta agatgaacgc tatcaggaac gccgggacgt ggtgcaggaa atgaagtttt
     2641 cagaagacgt ggacaacctt ataccttcaa gcagctctgg caccagttca ttatcaggaa
     2701 tgaccaggca gtatgacgtc gtggtggttg ggggcgggcc ggcagggagc acctgcggcc
     2761 ggctctgcgc ggagcagggg ctctccgtct gcctggtcga ggagcacggc gcggcgggaa
     2821 tccccgtcca gtgtgccggg ctcctctcga tgaatgcgtt cagggaatgc gaggtctcgc
     2881 agtcgagcat cctcgcgacg gtccggggtg cgaaggtggt ctcgagcctc ggcccggtgc
     2941 tcgaatttga cgcacgaatg ccaaaggccc atgtcgtaga ccgtgccgtg ctcgaccgcg
     3001 agatgcttgc ccgggcggcg ggcgccgggg ccgaggtcag gatgaagacc tcgtttgcag
     3061 accggaaagg ccaacgcatc gtcacacgcg gcgtgaatgg gaaggaggag atattgttca
     3121 ggatactcgt tgcagctgac ggagcacgga gccgggtggc gagggtgatg ggaatgcgcc
     3181 ggcccgaggt cttccttgcc ggtctgcagg cggacatcct gcacgggatg gacggatcca
     3241 gggtagagat tcaccccgat gcggcgccgg atttcttcgg gtgggcaatt cccatcggtg
     3301 cgggaagagc gagggtaggg ctcgcgggga ggactggtgt ccgcgagagg ttcaggagtt
     3361 ttctctcgct catttcacag gattcgcacc aggcgcaagt gcaccttgtc agcgggacca
     3421 tcccgctcgg gaccatggcc aggacctttg gggaccgggt gctttttgtc ggggatgccg
     3481 cggggatggc aaagccaaca tcagggggag gcgtgtatac cggtgtcagg gccgcacgcc
     3541 acgccgcgag ggtggcaggg gaggccgttg ccttggagga tttcggcgac gggatgctct
     3601 cccggtacga aagagcctgg aaggcgattc cttcgtcgag gtggttctgg atacaactcc
     3661 tgccgaggaa ctggcggccc tggttccggc gattctggcc cgcaatcaag cgcagttgtt
     3721 cagcagcgaa ctggccgatg cctacgccca gaatgcggcg gatacgctgc tgcatctggc
     3781 aacggtccgg aatccggttt tggacgtgat gaaggcggaa ccggcgctgc tggcgg
//
LOCUS       DAOQVJ010000099         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27177_length_3804_cov_3.768205, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000099 DAOQVJ010000000
VERSION     DAOQVJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27177_length_3804_cov_3.768205"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..762
                     /locus_tag="PKL26_03065"
     CDS             286..762
                     /locus_tag="PKL26_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82708.1"
                     /translation="MMILRISLLILACVLVLAAGCLGNAPGSPITTASPAPTTMPPRT
                     PTLSLVPEPTDIMPPTYSVTVQVVKNTIATNPTITVTYEGGQGLAFTQSMTAEVIRSD
                     GTVDQQTVQSPQMGSEIVLTGTTRTDRVIVYVTMVNGVTYRVFDKDMPFQPINPQY"
     gene            927..2081
                     /locus_tag="PKL26_03070"
     CDS             927..2081
                     /locus_tag="PKL26_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS82709.1"
                     /translation="MPRVTVYYTQNCPYCRMLKAFLEKHSVDYMAIDVGTDREQAGKM
                     VELSGQYGVPVTTVDDEVIVGFDAQRLNELFGTAREGDIYDVLIVGAGPAGLTAGVYC
                     SRKMVKTVIISENIGGQALESWAIENYMGYRMVTGEDLMHKFEEQVRGEHIHLELDRI
                     QAIRREGDLFVVDTATGGTYSTKTVILAQGKRPRKLGVEGEERYLGRGLSVCSTCDGP
                     LFRDKVVAVVGGGNSALQTAIEMSKIAREVHLVVRSTIKADEAYVKQYEQQGNIRTYL
                     HHAVAALHGNAMLNGITIKDRESGKETTISLDGVFAEVGWIPNTDFLEGFLRLNDQKE
                     IEIDINCHTSVPGVFAAGDVTNIRTKQIITAAGEGAKAALEAYDYVVKLE"
     gene            complement(2088..3329)
                     /locus_tag="PKL26_03075"
     CDS             complement(2088..3329)
                     /locus_tag="PKL26_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNS82710.1"
                     /translation="MSKRGSILHAMTGAGDLRFRGAGDVITESLSVYGIQVIRSTGEF
                     WTARQRQGSSLHEVSYRGCFKPQLPAYFINLLTKPGDLVYDPFSGRGTTVIEAGLLGR
                     AVAANDANPLSRLLTRPRFFLPDTADVKSRLEAIPIDTGAGAEMDLSMFFHPGTEAEI
                     SSLRNYLLERERDGDIDHVDDWIRMVATNRLTGHSSGFFSVYTLPPNQAVSPMRQIRI
                     NQKRDQVPPYRDTRALILKKTLSLLKDVSEIQKERLRKSGENALFLSGDSRRTPGIGD
                     GTVQLTITSPPFLDVVQYSSDNWLRCWFNGIDPGTVEKNLTVTRSLPDWRKVMQQTLY
                     ELFRVTRSGGHIAFEVGEVKGGTVRLDETVVPLGVRAGFSCEGILVNLQDFTKTSHIW
                     GIGNNAHGTNTNRIVVFKKEG"
     gene            3400..>3804
                     /locus_tag="PKL26_03080"
     CDS             3400..>3804
                     /locus_tag="PKL26_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82711.1"
                     /translation="MHEPSRKIVHMFFGVGIAALAYLLPKDLIVLLLAASIFIGFILA
                     EGVLRGYRIPLVSLLIRNLERDGEYPGKGAFYFAASALFCVLFFDAEISVPAILSLAV
                     LDGAATLVGLRWGRHRITNGKSVEGSAGGVLVN"
BASE COUNT          807 a         1106 c         1105 g          786 t
ORIGIN      
        1 aggtccatgc aacgctctac aaggaggcgc tcaataacct gaaccacggc caggatatgt
       61 ccaaccgcac ggtgtacatc tgccctgtct gcggcaatgt cttccttggg acggccccgg
      121 aaaagtgccc gatctgcatg gcagtccgaa aagtgttcag gacagttgaa taaccccatt
      181 cattttctct tgcgttctgt cttttgcgga ggagagagga ggtcccatgc ctgcgggtat
      241 aggcttcttg tgtattgata tagaacctgg agcagagata ttcccatgat gatcttgcgc
      301 atctctcttc taatccttgc atgtgtcctt gttcttgcag caggctgcct tgggaatgcc
      361 cccggttccc ccatcacgac agcctcacct gcaccgacga ctatgccccc tcgtacgccc
      421 acgttgtccc tcgtccccga gcctaccgac atcatgcccc ccacatactc ggtgacagtg
      481 caggtggtaa agaacaccat cgccaccaat cccaccatca cggtaacgta tgaaggaggc
      541 caggggctgg cattcacgca atccatgaca gcggaagtga tcaggtcaga tggaacggtc
      601 gaccagcaga ctgtgcaaag cccgcagatg ggatcagaga tcgtgctcac cggcacgacc
      661 aggaccgacc gggtaatcgt atacgtgact atggtcaacg gggttaccta cagggtgttt
      721 gacaaggata tgccattcca gccgatcaac ccccagtact gattttccta caaccccatt
      781 ttccaaaatc ccccgggaca aataagggat tggacggctc ctcttcttac cgggtattcg
      841 atccgggtat cccattcagg tgacaggaat cagctacaca ccccgctcca ccacgtttat
      901 tactcatccc cggcaaaccc cccatcatgc ccagggtcac cgtctactat acccagaact
      961 gcccctactg ccgcatgctg aaggctttcc tggaaaagca cagcgtcgat tacatggcaa
     1021 tcgacgtagg gaccgaccgg gagcaggccg ggaagatggt ggagctctcc ggacagtacg
     1081 gagtcccggt cacgaccgtc gacgatgagg tcatcgtggg atttgatgcc cagcggttga
     1141 acgagctctt cgggaccgca agggaaggag acatctacga cgtactcatt gtcggtgcgg
     1201 ggcccgcagg gctcacggca ggtgtctact gctcgaggaa gatggtaaag acagtcatca
     1261 tctctgagaa catcggggga caggcccttg agagctgggc gatcgagaac tacatggggt
     1321 atcgcatggt cacaggggaa gacctgatgc acaagttcga ggagcaggtc aggggagagc
     1381 acatacacct cgaacttgac cggatccagg cgatcaggag ggaaggggac ctcttcgtgg
     1441 ttgataccgc aacgggaggc acctattcca ccaagacggt catcctcgcc caggggaagc
     1501 gtcccaggaa actcggggtg gaaggggagg agcgctacct gggccgcggc ctttccgtct
     1561 gttcaacctg tgacggcccg ctcttccggg acaaggtggt cgccgtggtc ggcggcggga
     1621 actcggccct ccagaccgcg atcgagatga gcaagattgc acgcgaggtc caccttgtcg
     1681 tcaggagcac gataaaggca gacgaggcct acgtgaagca gtacgagcag cagggaaaca
     1741 tccgcactta ccttcaccac gcggtcgcag cgctgcacgg aaacgccatg ctcaacggca
     1801 tcacgataaa ggaccgcgag tccggcaagg agaccactat ttcgctcgat ggggtctttg
     1861 cggaggtggg atggataccg aacaccgatt tcctcgaagg attcctgcgt ctcaatgacc
     1921 agaaggagat cgagatcgac atcaactgcc acacgagcgt tcccggagtc tttgcggccg
     1981 gtgacgtgac gaatatcagg accaagcaga tcatcactgc cgcgggagag ggggcaaagg
     2041 cggcgctcga ggcctacgat tacgtggtaa aactggagtg aaccatctca tccctctttt
     2101 ttgaagacca ctatcctgtt ggtgttggtc ccatgggcgt tattcccgat gccccaaatg
     2161 tgggatgtct tggtgaagtc ctgcaggttc acgagtatcc cttcgcacga gaacccggcg
     2221 cggacaccga gcggcaccac cgtctcgtcg agtctgacag tccccccctt tacttcaccg
     2281 acctcgaagg caatatggcc cccgctcctc gtgacgcgga agagttcgta cagcgtctgc
     2341 tgcattactt tcctccagtc ggggagtgag cgggtgacgg tgaggttttt ttccacagtt
     2401 ccggggtcta tcccattgaa ccagcaacgg agccagttgt cggacgagta ctggaccacg
     2461 tcaaggaacg gaggagaggt gatggtaagc tggacggtgc cgtcaccgat ccccggagtc
     2521 cgccgcgagt cgcccgaaag gaagagggca ttctcccccg atttccgcag gcgttccttc
     2581 tggatttcgc tcacgtcctt caggagggaa agggtctttt tcaggatcag agctcttgtg
     2641 tcccggtatg gcggtacctg gtccctcttc tggttgatcc gtatctgccg catgggtgag
     2701 actgcctggt tcggcgggag cgtgtagact gagaagaagc cgcttgaatg gcctgtcagc
     2761 ctgttggtgg cgaccatgcg tatccaatcg tcgacatggt cgatgtcccc gtccctctct
     2821 ctctcgagca ggtagttgcg aagagaggag atctccgcct ctgtcccggg gtgaaagaac
     2881 atggagaggt ccatctcggc ccccgcaccg gtatcaatgg gtatcgcctc gagccttgac
     2941 ttcacatcgg cagtatcagg cagaaagaaa cgggggcggg tgaggaggcg cgagagtgga
     3001 ttggcgtcgt ttgccgcaac agccctcccc agcaacccgg cctcgatgac ggtggtcccc
     3061 ctcccgctga aggggtcgta taccaggtca cctggtttcg tcaggaggtt gatgaagtag
     3121 gcgggcagct gcggcttgaa gcagccgcgg taggagacct cgtggaggga tgaaccctgc
     3181 cgctgcctcg cggtccagaa ctcgccggtg ctgcgtataa cctggattcc atataccgaa
     3241 agggattcgg tgatgacatc tccggctccc ctgaaccgaa ggtcgcctgc ccctgtcatt
     3301 gcatgtagta ttgagcccct tttgctcatc aacctctcca cccgggatga aggatcgcaa
     3361 tccgaccagt taatggtccg ggatacccat actctgggca tgcacgagcc gtcccggaag
     3421 attgtccaca tgttcttcgg agtgggaatt gccgcacttg catatctcct cccaaaagac
     3481 ctgatagtcc tccttctcgc ggcctcaata tttatcgggt tcatcctcgc cgaaggggtg
     3541 ttgcggggat atcgtatccc gctcgtttcc cttctcatcc gcaaccttga gcgcgacggc
     3601 gaataccccg ggaaaggggc tttctacttc gccgcaagcg ccctcttctg cgtactcttc
     3661 ttcgatgcgg aaatttcggt ccctgcaata ctctcgctcg cggtccttga cggcgcagcc
     3721 acgctcgtgg gactgcggtg ggggaggcac cggatcacga acgggaaatc tgtggagggc
     3781 tcggcaggag gggtgctcgt caac
//
LOCUS       DAOQVJ010000100         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27455_length_3776_cov_4.691750, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000100 DAOQVJ010000000
VERSION     DAOQVJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27455_length_3776_cov_4.691750"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1666
                     /locus_tag="PKL26_03085"
     CDS             41..1666
                     /locus_tag="PKL26_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit A"
                     /protein_id="HNS82712.1"
                     /translation="MRSAEVLDCHVLVVGSGGAGVRAAIEASGYGETVLVSKTLTGKG
                     GCTTMAEGGYNAVLREQDSCEIHFEDTMKGGAYLNDPDLVRILVDEAPKRIDDLLQWG
                     AVFDVTDCCEVAQRPFGGQRFPRTCYAGDRTGHEMMATLVERLAGTDVDVMNEVCALD
                     LLKDGDRVLGAVGLTEKDELVAIRADSTVLATGGGTRIYDISTNSSSGTGDGFAIGYR
                     AGAELIDMEMVQFHPTGAVYPYDARGRLITEAVRGEGGVLKNSLGERFMNRYDPERME
                     LSTRDVVSRAIATEILEGRCTGHGGVYLDVTHLPRQQIEEKLPVMLEQFLKFGVDIRE
                     EPMEVAPTAHHMMGGHRITENGETTISGLYACGEVAGGVHGANRLGGNALADTQVFGK
                     RAGEAAGKSGKKSGRIDQRAMDAQEARLSAFFDGEVVPSTVARSLRTAMWEGAGIFRT
                     AKELEKTLGVINHLSSLRLKASGRSNLAECCILQNMLTTASLVVKGALIRKESRGAHV
                     RKDVTQTWDPQTSPYGHSYQSLTQSGIETGVKA"
     gene            1663..3126
                     /locus_tag="PKL26_03090"
     CDS             1663..3126
                     /locus_tag="PKL26_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNS82713.1"
                     /translation="MKKMNVRIQRFDPHQDEKPHYQTYLVEVNEGARVLHVLHAIHDE
                     IDPTLSYRWCCGSGQCGSCAVRVNGEPVLACMEEARDGMTIEPLKLPVKKDLIVDLEP
                     ALEALSHLEPGDKPAMPSKEEVELIKPLRSCIECLACVSVCPAMDVTSFAGPTAMRQN
                     LRLALDPRDSKDRVTDAVSKGLFTCTSCQACWKVCPKDIQLPGKAIEKLRSLANRKGL
                     TLPRHQEVEKLVRETGRSVTRTEPTFMEQVGEVIEPYGPVKATVGFFVGCMYNQRLPQ
                     TALDAMEVLRRNGIRVIIPKEQVCCGSPLIRTGQWDYLDTLKRRNIDAFVFRNIDTVL
                     TMCAGCGSTLKNDYRTPFRVLDINELLTKYGIEPPARLPLKATYHDPCHLMRGQGIRD
                     LPRELITQVVDLVEMPAICCGSGGGVRSGVPEEAAALAEKRREEIEKTGADIVVSSCP
                     FCEFHIMEHTDRPVKNITTLLLEGYREKDKKKAEKAV"
     gene            3220..3453
                     /locus_tag="PKL26_03095"
     CDS             3220..3453
                     /locus_tag="PKL26_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82714.1"
                     /translation="MTTMTNPWLPYNIPPMRAYILAPGIVQIEDTVYNLPSRSVEHGR
                     PAPVKPEFIVPVKENSEKAARDAMEGLYRIVFD"
     gene            complement(3487..>3776)
                     /locus_tag="PKL26_03100"
     CDS             complement(3487..>3776)
                     /locus_tag="PKL26_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82715.1"
                     /translation="QAGAIVLREARKGYIPMGVFNVRENVRQAMHQPFHEFEDLPSAL
                     ADVSKRMTLPMGRFVNESTVLKSQFRARQTTLDSFFRPGKSSVCIPPDSTA"
BASE COUNT          841 a         1062 c         1182 g          691 t
ORIGIN      
        1 caagctattt atgtacgtat atgacccacc ttataataag atgcggtcag ctgaagtatt
       61 ggactgccat gtcctggtgg ttgggagtgg cggggccggg gtccgggctg ccatcgaggc
      121 ctccggctac ggggagactg ttctcgtctc aaagaccctc accggaaaag gtgggtgcac
      181 gacaatggcc gagggaggct acaacgcggt gctcagggag caggactcct gcgagatcca
      241 cttcgaggac acgatgaagg gtggtgcgta cctgaacgac cctgatctcg tacgaatcct
      301 ggtggacgag gcaccgaaga ggatcgatga cctcctgcag tggggagcgg tctttgacgt
      361 gaccgactgc tgcgaggtgg cccagagacc attcgggggg cagaggtttc cccgcacgtg
      421 ctatgccgga gaccgcaccg gccacgagat gatggcgacc ctcgtagagc ggctcgcggg
      481 aacggatgtc gacgtgatga acgaggtttg cgcgcttgac ctcctgaagg atggcgatag
      541 ggtacttggg gcggtggggc tcaccgagaa ggacgagttg gtcgctatcc gtgccgacag
      601 caccgttctt gcaaccggtg gcgggaccag gatttacgac atatccacga actcctcgag
      661 tggaacaggc gacggtttcg caatcgggta ccgtgcaggg gccgagctga tcgacatgga
      721 gatggtccag tttcacccga caggcgccgt gtacccatac gatgcaagag gccggctgat
      781 caccgaagcg gtaagggggg aaggtggcgt cctgaagaac agcctcggcg agcgcttcat
      841 gaaccgttat gaccccgaga ggatggaact ctccacccgc gacgtggtgt cccgtgcaat
      901 cgcgaccgag atccttgagg ggcggtgcac tggccatggc ggggtctacc ttgacgtgac
      961 ccacctcccg cggcagcaga tcgaggagaa gctccctgtc atgctcgagc agttcctcaa
     1021 gttcggggtg gacatccggg aagaaccgat ggaggtcgca cccactgccc accacatgat
     1081 ggggggccat agaatcacag agaacgggga gacgaccatt tccggcctgt atgcatgcgg
     1141 tgaagtggcc ggcggggtcc atggcgccaa ccggctggga ggaaatgccc tcgcagacac
     1201 ccaggtgttt gggaagaggg ctggagaggc tgcaggaaag tcaggaaaga agagtggacg
     1261 tatcgatcag agggccatgg atgcgcagga ggcaaggctc tccgcgttct ttgatggcga
     1321 ggtcgtgccg tccacagtcg cacgatcgct ccggactgcc atgtgggagg gggcagggat
     1381 attcaggacc gcgaaggaac tcgagaagac actcggggtg atcaatcacc tttcttcatt
     1441 gcgcttaaag gcttccgggc ggtccaacct tgcggaatgc tgcatcctgc agaacatgct
     1501 cacgactgcc tcgctggtgg tgaaaggggc gctgatcagg aaagagtcgc ggggtgccca
     1561 cgtccgcaaa gacgtgaccc agacgtggga cccccagacc tctccctacg ggcacagtta
     1621 ccagtcgctc acgcagtcgg gaatcgagac gggggtgaag gcatgaagaa gatgaatgtg
     1681 aggatccaga ggttcgaccc ccatcaggac gaaaaaccgc actatcagac gtacctcgtg
     1741 gaggtcaacg agggagcacg tgtcctccac gtcctccatg ccatccatga cgagatcgat
     1801 cccacgctct cctaccgatg gtgctgcggt tccgggcagt gcggaagctg cgccgtccgg
     1861 gtcaacgggg agccggtgct ggcctgcatg gaagaagcca gggacgggat gaccatcgag
     1921 ccgctgaaac tcccggtgaa aaaggatctc atcgtcgacc tcgagcctgc gcttgaggct
     1981 ctttcgcacc tcgagcccgg tgacaagccg gcaatgccga gcaaggaaga ggtcgaactc
     2041 atcaagccgc ttcgaagctg catcgagtgt ctcgcctgcg tctctgtatg ccctgcgatg
     2101 gacgtgacct cctttgccgg gccgaccgcg atgcggcaga acctccgcct cgcgctcgat
     2161 cccagggatt caaaggaccg ggtgacggac gcagtctcga aggggctgtt tacctgcacc
     2221 tcatgccagg cctgctggaa ggtatgccct aaagacatcc agcttcccgg caaagccatt
     2281 gagaagctgc gttccctcgc gaacaggaag gggctgacac tcccgcgcca ccaggaggtc
     2341 gagaaactgg tgagggagac cgggaggagc gtcacccgca cagagcccac cttcatggag
     2401 caggtcggag aggtgatcga gccgtacggc ccggtgaaag ccactgtggg gttcttcgtc
     2461 gggtgcatgt acaaccagcg gctgccacag accgcacttg acgcaatgga agtgctgcgc
     2521 cggaacggca ttcgtgtcat tatcccaaag gagcaggtct gctgcggatc accgctgatc
     2581 aggacgggcc agtgggacta cctcgatacc ctcaagagga ggaacatcga cgcgttcgta
     2641 ttccggaata tcgacactgt gctcaccatg tgcgccggat gcgggtcaac cctcaaaaat
     2701 gactacagga ctcccttcag ggtgctcgac atcaacgagc tgctgaccaa atacgggatc
     2761 gagcccccgg cgaggctgcc cctgaaggcc acctaccacg acccatgcca cctgatgagg
     2821 gggcagggga tccgggatct gccgcgtgaa ctgatcacgc aggtcgtgga tcttgtagag
     2881 atgccggcca tctgctgcgg gtctggcggt ggcgtaagat cgggagttcc cgaggaggcc
     2941 gcagcgctcg cagagaagcg gagggaagag atagagaaga caggggcaga tatcgttgtc
     3001 agctcctgcc cgttctgcga gttccatatc atggagcaca ccgaccggcc ggtgaagaat
     3061 atcaccaccc ttctcctgga aggctaccgt gagaaggaca aaaaaaaggc cgaaaaagct
     3121 gtgtgatcgg acttctatct cttttttccc tgtttaatga agttatccca tcaacattac
     3181 accttcacaa cattctttgt catgtagcca tcacactgca tgacgaccat gactaatcca
     3241 tggctcccct acaacatacc cccaatgagg gcgtacatcc tcgccccggg tatcgtgcag
     3301 atcgaggata cggtctacaa tcttccctcg cggtcagtag agcatggccg gcctgcaccg
     3361 gtaaaacctg aattcatcgt gcctgtaaaa gagaacagcg agaaggcagc tcgtgatgcc
     3421 atggaggggc tgtaccggat cgtgtttgat tgagaggtgg ggacctcggg atgtcccgga
     3481 aagatcttat gcagtggaat ccgggggaat gcatacactg ctctttccag gtctaaaaaa
     3541 agaatcaagt gtggtctgcc tcgcccggaa ctgtgacttc agcaccgtgc tctcgtttac
     3601 gaacctcccc atcggaagag tcattcgctt cgatacatca gcgagagccg aggggaggtc
     3661 ttcgaattca tggaacggct ggtgcatcgc ctgccttaca ttctccctga cattgaagac
     3721 ccccatgggg atatacccct tccgggcctc ccgaagcacg atcgccccag cctgca
//
LOCUS       DAOQVJ010000101         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27530_length_3768_cov_3.914086, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000101 DAOQVJ010000000
VERSION     DAOQVJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27530_length_3768_cov_3.914086"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1507)
                     /locus_tag="PKL26_03105"
     CDS             complement(380..1507)
                     /locus_tag="PKL26_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HNS82716.1"
                     /translation="MYQGRTLVLSVDRDDDIGFKASINSPVIGRDACLAAANTLGLAD
                     PEDSDVIAIFQAIKIYDELLGRGEDVEVAVIAGNHMHMIEGDRRIAASLGDVVAQTGA
                     SNCIVVTDGAEDEFVIPIVQSKLPVSSIRRVVVSQMPNLEGTYYIIKKLLNDPKVARL
                     VLVPLGLAMLLWAVAYLVDMPQIATFIVIGAIGIYLLYRGFGVDEIFEGFAHALRTSL
                     TRGKFSFITYIAGILLVIVGVIMGLMNLLVYYAEIGLLLYFLIFMYGAVLWLALAGVV
                     SSIGIIIDNYFYDRVGLAKVIVFPFFIGAVGLITYGACIYAISMNNIPDFPYSTSTAV
                     QFIFYGTLGGLICALSGIGIQYMVNKYLASQRSGESVELVS"
     gene            complement(1568..2428)
                     /locus_tag="PKL26_03110"
     CDS             complement(1568..2428)
                     /locus_tag="PKL26_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HNS82717.1"
                     /translation="MLNELIEKRKKILAESEQHKNRRNELNALASKGARERNTLNNQT
                     REYVEEAQKNKELRDKYNNDVQAIKDERNQINEQANAFFEEIESFKKEHGGMRNRGLK
                     ELQKQIEHLEFRQQTEVFSTDKERELIEKIKQMKESVREQEAELEQNKEIKAKISSAR
                     DLRKQASDLHAKVTEMAELAQKHHDLMVDFYRKADKSREEADAAHKNFVEAQESADAE
                     HKYFIACQKELRDYDKVISGLRKKTKKAKVTKEQKAVRKEAEHLFKMFRAGEKLTTDD
                     ILLLQRSKLI"
     gene            complement(2922..>3768)
                     /locus_tag="PKL26_03115"
     CDS             complement(2922..>3768)
                     /locus_tag="PKL26_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315264.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNS82718.1"
                     /translation="MARPTLEALLENADLCFITAGMGGGTGTGSAPVVAQIAKEQGAI
                     VVGMVSYPFQVEKARLIRAEEGLESLASAADSVIVLDNNRLKNFVPNLPLGQAFSVMD
                     QLIGETVKGISETITQPSLINIDYADVRAIMSKGGVAVMLVGESKQQNKSESVVRECL
                     SNPMLDIDYRGATGSLIHITGGGDLTLQDAEEIASSLTYELDPHADVIWGARVKNDME
                     GKVRVLAIMTGVQTGKIMGSRVSYKTILEDLEQKKVSPKAPQLPRNRKARDQTAGGTL
                     LEWVG"
BASE COUNT          798 a         1030 c         1007 g          933 t
ORIGIN      
        1 ccaagatggt cccgaatggc atcgggtatt ttgataatcc tgaatgattg gaggccggag
       61 gcctcttatc ctgaatgatt ggaggccgga ggcctcttat cctgaatgat tggaggccgg
      121 aggcctctta tcctgaatga ttggaggctg gggggcattt tattcatccc cgctgccaga
      181 aaattaaaaa accgggtgtt tccttgattt tccggcgaca tgcatgaccg gataccttgt
      241 gatctcaatg caggacgaca ggatttatag gaaaggtgag agatccgagg gacacctttc
      301 aggggagcct ccgtcattga gagggtggaa gggggaggga caggctgtcc cggagagaga
      361 gacctggggg ccgcaatgtt taggagacga gctctacaga ctctccgctt cgctgtgaag
      421 caaggtattt gttcaccatg tactggatcc caatacctga aagggcgcag atcaggccgc
      481 ccagggtgcc atagaatatg aactggaccg cagtgctggt gctgtaaggg aaatcgggga
      541 tattgttcat agagatggca taaatgcagg ccccgtacgt gatcagccca accgcaccga
      601 tgaaaaaggg gaagacaatc acctttgcaa ggccgaccct gtcatagaaa tagttgtcaa
      661 tgatgatccc tatcgaggag acaacgccgg caagtgccag ccatagcacg gcgccataca
      721 tgaatatgag gaagtagaga aggaggccga tctccgcata atagacgagg aggttcatca
      781 gccccatgat cacgcccacg attacgagga gaatccctgc gatatatgtg atgaaagaga
      841 acttcccgcg ggtaagagag gtgcgaagtg cgtgtgcaaa tccctcgaat atctcgtcga
      901 ctccaaaacc cctgtagagg agatagatgc cgatcgcacc gatcacgatg aaggtggcga
      961 tctgtggcat atcgacaagg tacgcgaccg cccagagaag catcgccagt cccaggggga
     1021 caagcacaag cctggccacc ttcgggtcgt tcagaagctt cttgatgata taataagtgc
     1081 cctcgaggtt gggcatctgg cttaccacca ctctccggat gctcgaaacg gggagttttg
     1141 actggacgat ggggatgaca aactcgtctt ctgcgccatc cgtgaccacg atacagttcg
     1201 aggcccccgt ttgcgccact acgtccccga gagatgcggc aatcctccgg tcaccctcga
     1261 tcatgtgcat gtggttcccc gcgatgactg ccacctcgac atcttctcca cgtccgagca
     1321 actcgtcgta tatcttgatg gcctggaaga tggcgatcac atcggagtct tcgggatccg
     1381 caagtccaag ggtatttgcg gccgcaagac aggcatcacg cccaatgaca gggctattta
     1441 tacttgcctt aaaaccgata tcatcgtcac ggtccacgga aaggacaagc gtgcgcccct
     1501 ggtacatcaa tatagaagga gcaagctatc tattaaatga ttgctttccc agggtcaaaa
     1561 aaaggaatta aatgagcttt gacctctgca ggagcaggat atcgtctgtc gtgagtttct
     1621 ctcctgcccg gaacatcttg aaaaggtgtt cagcctcttt ccgcacggcc ttctgctcct
     1681 tggtgacctt tgccttcttg gtcttcttgc ggagacccga gatcaccttg tcatagtcgc
     1741 ggagttcctt ctggcaggca atgaaatact tgtgttcggc atctgctgac tcctgcgctt
     1801 cgacgaaatt tttgtgggcg gcgtccgcct cctccctcga cttgtcggct ttccggtaaa
     1861 aatcgaccat caggtcgtgg tgtttctgcg caagctctgc catctcagtg acctttgcat
     1921 gcaggtcgga tgcctgtttc cggagatccc ttgcagagga gatcttggcc ttgatctcct
     1981 tgttctgctc gagctccgcc tcctgttccc gcaccgactc cttcatctgc ttgatcttct
     2041 cgatcaactc gcgttccttg tcggttgaga agacctcggt ctgctggcgg aactcgaggt
     2101 gctcaatctg cttctggagc tcttttaagc cccggttgcg catcccgcca tgctctttct
     2161 tgaaggactc gatctcctcg aagaaggcgt ttgcctgctc gttgatctgg ttgcgctcgt
     2221 ccttgatggc ctggacgtca ttgttgtatt tgtcgcgcag ttccttgttc ttctgcgcct
     2281 cctcaacgta ctccctggtc tggttgttga gagtgttcct ctcccttgcc cccttgctcg
     2341 caagggcgtt gagctcgttc cttctgttct tgtgctgttc agactcggca agaatcttct
     2401 ttcgtttctc gatcagttca ttcaacatgc tctaccatcc tcgacggata cgtcggaatg
     2461 cccgaatccc acgcaagttt aggaaaatgc caaagaaaag atggacacac aatgcagaaa
     2521 tttggagatc ttgcctctgt ccggagcact ccgtggagag aacgcccacg ttcatgcatc
     2581 ccacggctcc attgaaaaaa gcgccacggc acctggccgg gcctttgttc atacggctgt
     2641 ccatcatgtt cgactcccga ttccggaagg ggactatcag gttccctttc agccaccctt
     2701 ctctggcaat cccggatgca tggaatccgg tgattttcta ttatttacac gatatctatc
     2761 ctattaattg tttcgccagg gcggagaaaa acggccttgg aatggtcttc acagcgcagg
     2821 aaatcatgaa aaaagtatgg ggaacaggaa gccggtatta ccggctttca cagttctata
     2881 ttggcagttg tgtgaatcaa ggtggtttgt gcagtgctga tctaacctac ccactcgagt
     2941 aaggtacctc ctgccgtctg gtcacgtgcc ttcctgttcc tcgggagctg tggtgctttc
     3001 gggctcacct tcttctgttc gaggtcctca aggattgtct tgtaggagac cctgctcccc
     3061 atgatctttc ctgtctggac cccggtcatg atggcgagta ccctgacctt gccctccatg
     3121 tcgttcttca cccgggcgcc ccagatgaca tctgcgtggg ggtcgagctc gtaggtgagg
     3181 gaagaggcga tctcctctgc gtcctgcagg gtgaggtcgc cgcccccggt gatgtggatg
     3241 aggcttcccg tagcgccgcg atagtcaatg tcgagcatcg ggttgctcag gcactcgcgg
     3301 accacgctct cggacttgtt ctgctgtttg ctctccccga ccagcatcac cgccacacct
     3361 cccttgctca tgattgccct gacatcagcg tagtcaatgt tgatcagcga aggctgggta
     3421 atggtctcgc tgatcccttt cacggtctca ccgatgagct ggtccatcac tgagaacgcc
     3481 tgtccgagcg ggaggttggg tacaaaattt ttcagcctgt tgttatcaag gacaataacc
     3541 gagtctgcgg cggaagcaag ggattcgagt ccctcttctg cccgtatcag gcgggctttc
     3601 tcgacctgga aggggtagct taccatgccg acgacaatcg caccctgttc cttggcaatc
     3661 tgtgcaacca cgggtgcgga accagtcccg gttcctccgc ccatacctgc agtgatgaaa
     3721 cagaggtccg cgttctccag gagtgcttca agagtgggcc tcgccatc
//
LOCUS       DAOQVJ010000102         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27613_length_3760_cov_4.016464, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000102 DAOQVJ010000000
VERSION     DAOQVJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27613_length_3760_cov_4.016464"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1293
                     /locus_tag="PKL26_03120"
     CDS             151..1293
                     /locus_tag="PKL26_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HNS82719.1"
                     /translation="MEAMRSYERYGLLLNERVVKTPIAVSAMAGIVDAEYALERAAHI
                     GAAFLGGYSIDGPTMEASRTLAAAGRPEFLYEDPAEALAAQVSHMAPSDVVCGLNLRA
                     GSPDSLAVIASQIGDSVIYEIDAHCRQEPLVRARCGEYLLHHPDLLGEYIRALKREEV
                     SVSVKMRAGVSADDLLLVKKIWGAGADIIHVDLMDFGYQKVRQIRNNSPLFVIANNSV
                     NTFEKMKDMLSHGADMVSLARQSDERTLAGLDAAISRFTDEHGWYNAPKQLCRGGDIR
                     SLAFCCMPVKNCPLIPMLEKIGLSREDYLSFKQNAVAGTPLDFGSQTCFGSLAWCCKD
                     SSPCMFREMTLRQAGLSNREYMRQKKQLADKIMQHLFYEDAATHPC"
     gene            1265..2083
                     /locus_tag="PKL26_03125"
     CDS             1265..2083
                     /locus_tag="PKL26_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNS82720.1"
                     /translation="MRMRPLTRADRAQLAMILEVSAYPKPGNVDRCHDYETTRLEHFL
                     ASAILARPALEAAERSEGGPGALIHQAVECTSGHRGGNTHFGAFILLIPLLMGGDIPG
                     ATRVVGSTTVDDAVEFYRAFGKTEVRVIQGHELDVHDPSSIMEIRSRGMTLYDLMLFS
                     APRDMVAREWINGFEMTRRGADLIHAAGSGQQAVVEAFLGLLSLEPDTFVIKKHGPDV
                     ASKTMEKAREVREGLRDLQAFDQECIDKKINPGSIADIIIAALYIALGEGWEWD"
     gene            2074..2646
                     /locus_tag="PKL26_03130"
     CDS             2074..2646
                     /locus_tag="PKL26_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HNS82721.1"
                     /translation="MGLIRDGINEVIATTHWNAAPMGIISREGSQRMVVFNGSHTAEN
                     IRRHGWVVANITFDPVVYVETAFGDIGRERFVPEKIGDLWVHRLAGAEAWAVFSSRVS
                     RETEEALVVDLTPLYEQMLTCAVHPVNRGFSSIIEATVHATRYVAKKDPALMQLIEHH
                     ASIVRRCGGKREMEALSLLQTYLPKGKGFS"
     gene            complement(2648..3643)
                     /gene="hypE"
                     /locus_tag="PKL26_03135"
     CDS             complement(2648..3643)
                     /gene="hypE"
                     /locus_tag="PKL26_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNS82722.1"
                     /translation="MKVNLMHGAGGEVMGELLQILTRFEHRNAGGVGLESLDDGAVIP
                     IGGVNIVFTTDSHVVRPIFFPGGDIGRIAVCGTVNDLAMMGGKPIALSCGMIIEEGFE
                     SSDLERIVRSMDTALGECGASLVTGDTKVLERGALDGIAVNTAGIGIADRIIRDNGLQ
                     PGDRILVNGTLGDHGMTIMAERAELSLGGQLVSDVAPLWGIVEKAMAAGEIHAMKDPT
                     RGGFASAINEMGRKSNVSVRISEADLPIRRSVRSVADMLGIDPLQVANEGKVVMGVPA
                     RDAEAILEAMRSHPYGREAAIIGEVVAGSHVIMETSVGGERFIEPPVGDPVPRVC"
     gene            complement(3640..>3760)
                     /locus_tag="PKL26_03140"
     CDS             complement(3640..>3760)
                     /locus_tag="PKL26_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNS82723.1"
                     /translation="TCGYEGTERFVCPRCGGLPELLKGREILVTNIEIEVNDE"
BASE COUNT          806 a         1094 c         1075 g          785 t
ORIGIN      
        1 ttttccaaat aacaagtatt tagaggtttt ttccaggccc ctcctcagca gagggaactt
       61 cagctgggca tccagctttt tatatccatg aaaaattaaa tttataagaa cttcggggac
      121 cgcgattttt ccggagatga gagtcgtgcc atggaagcga tgcgcagtta cgagaggtat
      181 ggcctgctgc tgaatgaacg ggtggtaaag accccgatag cggtctcagc tatggcggga
      241 attgttgacg cggagtatgc actggagagg gctgcacaca ttggagctgc gttcctgggt
      301 ggttactcaa tcgatggacc caccatggaa gcaagccgga ccctggcagc agcaggacgc
      361 ccggaatttc tctacgaaga tcccgcagag gcactggctg ctcaggtatc gcacatggct
      421 ccctcagatg ttgtctgcgg attgaacctg agggcaggca gtccggattc gctcgcggtc
      481 atagcctcgc agataggtga cagcgtcatc tacgagatcg acgcccattg ccgtcaggag
      541 cctcttgtcc gggcacgctg cggcgaatat ctcctccacc accctgatct tctgggtgaa
      601 tacatacgcg cactgaagcg agaggaggta tccgtatctg tcaagatgag agcgggggtc
      661 agtgcggatg acctcctgct ggtaaaaaag atatggggag caggtgccga catcatccac
      721 gtggacctga tggatttcgg gtatcagaaa gtgcgccaga tccggaacaa cagcccactg
      781 tttgttattg ccaataactc cgtcaatacc tttgagaaga tgaaggacat gctctcccat
      841 ggagctgaca tggtgtcact cgcccgccag tccgacgaac ggacactcgc aggcctcgat
      901 gcggcaatct ccaggttcac cgatgagcat gggtggtata acgccccaaa gcagctctgc
      961 aggggaggag atatcaggtc acttgccttt tgctgcatgc ctgtcaagaa ctgccctctg
     1021 atcccgatgc ttgaaaagat tgggctctcc cgggaggact acctctcatt caagcagaac
     1081 gcggtagccg gaacccccct tgatttcggc agccagacct gttttgggag ccttgcctgg
     1141 tgctgcaagg actcatcccc ctgcatgttc agggagatga cgcttcggca ggccggactc
     1201 tcgaacagag agtacatgcg acagaagaaa cagctggctg ataagatcat gcagcatctc
     1261 ttttatgagg atgcggccac tcacccgtgc tgaccgtgcg cagctggcaa tgatactgga
     1321 ggtctcggcg tacccaaagc cagggaacgt cgaccgctgc catgattatg agactacgag
     1381 gctcgaacat tttcttgcat ccgcgatact agcccgcccg gcactggagg ctgcggagag
     1441 aagcgagggt ggcccaggtg ccctcatcca ccaggcggtg gaatgcacca gcggccaccg
     1501 gggaggaaat acccattttg gggcattcat actccttata cccctcctga tggggggaga
     1561 tattcccggt gcaacgaggg tcgtcggcag caccactgtt gatgatgccg tggagtttta
     1621 ccgcgcgttt ggaaagaccg aggttcgtgt tattcagggt cacgaactcg atgtgcacga
     1681 cccctcatcc atcatggaaa tacgatcccg gggcatgacc ctctatgacc tcatgctttt
     1741 ctctgcaccc agggacatgg tggcaaggga gtggatcaac ggcttcgaga tgacacgcag
     1801 aggcgcggac ctcatccacg ctgccgggag cggccagcag gcggtggtcg aggcgttcct
     1861 tggcctgctt tccctcgagc cggatacgtt cgtcatcaaa aagcatggcc cggacgtggc
     1921 atcaaagacc atggagaagg caagggaggt tcgcgaggga ctgcgcgatc tccaggcatt
     1981 cgaccaggag tgcattgaca agaagatcaa cccgggatcc atcgcagata ttatcattgc
     2041 tgccctctac atagcccttg gagagggatg ggaatgggac tgataaggga cgggatcaac
     2101 gaggtgattg cgaccactca ttggaatgcg gcccctatgg ggatcatcag ccgcgagggg
     2161 agtcagagga tggtggtgtt caacggaagc catactgccg aaaatatccg ccgtcatggc
     2221 tgggttgtcg cgaacatcac cttcgacccg gtggtgtacg tggagacggc gttcggggac
     2281 attggcaggg agcggtttgt ccccgagaag attggggatt tatgggtgca tcgtctcgcc
     2341 ggtgcggagg cctgggcagt gttctcatca agggtgagcc gggagacaga agaggctctg
     2401 gtggtggatc tcactcctct ctatgaacag atgctcacct gcgcggtgca tccggtgaac
     2461 aggggttttt ccagtattat tgaagcaaca gtccacgcaa cgcggtacgt ggcgaagaag
     2521 gaccctgcac tgatgcaact catcgagcac catgcatcta ttgtccgcag gtgcggggga
     2581 aagcgggaga tggaggccct ctcgctcctg cagacgtacc tcccaaaagg aaagggcttt
     2641 tcctgaatca gcagacccgc ggaaccgggt ctcccaccgg gggttcgata aaacgctcgc
     2701 ccccgacgga tgtctccatg atcacgtggg acccggcgac cacctcgccg atgatggcag
     2761 cctctctccc ataagggtgc gaacgcatcg cctcgagtat cgcttcggca tcgcgtgcag
     2821 gaacccccat caccactttt ccttcgtttg cgacctggag agggtcgatc ccaagcatgt
     2881 cggcaacgct tctcacgctt ctcctaatcg ggagatctgc ctccgatatc cggacagaga
     2941 cgttgctctt cctccccatc tcgttgatgg cgctggcaaa tccccctctc gtcgggtctt
     3001 tcattgcatg gatctcgcct gcagccatgg ccttctcgac gatcccccac agcggtgcga
     3061 catcagatac gagctgcccg ccgagggaga gctcggcccg ttccgccatg atcgtcatgc
     3121 catgatcccc cagtgtcccg ttcacaagga tacggtcgcc cggctggagg ccgttgtccc
     3181 tgatgatccg gtcggcaata ccaatgcctg cggtattcac cgcgattccg tcaagtgcac
     3241 ccctctcaag gaccttcgtg tcacccgtga cgagacttgc cccgcactcg ccgagtgccg
     3301 tatccatgga ccggacgatt cgttcaagat cggaactctc gaagccctcc tcgatgatca
     3361 tcccgcacga cagggcaatg ggctttccgc ccatcatggc aagatcgttg accgtcccgc
     3421 atactgcgat cctcccgatg tccccgccag ggaagaagat aggccggacc acgtgggagt
     3481 cagtcgtgaa cacgatgttt acccccccga tcggtatcac ggcaccatca tcgagtgatt
     3541 caagccccac gccccctgca ttccggtgct cgaaccgcgt gagtatctgg aggagttccc
     3601 ccatcacttc accccccgcg ccgtgcatca ggttgacctt cattcgtcgt tcacctcgat
     3661 ctcgatattg gtcaccagta tctctctccc cttcaaaagt tccggcagcc cgccgcaccg
     3721 ggggcatacg aaacgctccg tcccctcgta cccgcatgtg
//
LOCUS       DAOQVJ010000103         3745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27764_length_3745_cov_3.532249, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000103 DAOQVJ010000000
VERSION     DAOQVJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27764_length_3745_cov_3.532249"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKL26_03145"
     CDS             <1..399
                     /locus_tag="PKL26_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82724.1"
                     /translation="ETPDNSGDNPQRKGKERKGKESIAAPVITPEETEEKEGPFELPS
                     KEDIVESSPSWIRDKIDEVCKRLVRENIYPEAVNYVLAKRNMRVSERALLHALCRVHI
                     SKPKHPKAFMDKIIAIENGNYNEYEHCQSS"
     gene            371..871
                     /locus_tag="PKL26_03150"
     CDS             371..871
                     /locus_tag="PKL26_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNS82725.1"
                     /translation="MNTSIVSLHEKIIGEFMSLAEKMAAEGCSQQDIMKAFRNYISLK
                     TRKTHKKPPMQKASSVIGDIAKSRRDADSNTERILHDMMVESGIRFKFQYRIGPYRVD
                     YLIGSTIIELDGPHHNARKLQDQVRDKYLRKRGYEIIRIPTYIFNVAPDAVLAEIKGM
                     EGKTPF"
     gene            1005..1310
                     /locus_tag="PKL26_03155"
     CDS             1005..1310
                     /locus_tag="PKL26_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82726.1"
                     /translation="MKKIGVLIAIMLALIIGYRQGQAVEFEYDGRVEYKFRFSEKEMR
                     EILTEHLVEKGYEIPPDGEEYISHIGTRTIFTIHRKILAPPAIKMPEYIRSERGAEP"
     gene            1307..1588
                     /locus_tag="PKL26_03160"
     CDS             1307..1588
                     /locus_tag="PKL26_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82727.1"
                     /translation="MKKAIITIGIIVTILVVSPAYANDKKTKDTTPALPTKQELVWEK
                     KYLEERFNALTANITLSQQEISAISVRYQEVERLLKAIEEQEKPKGKGK"
     gene            1585..1953
                     /locus_tag="PKL26_03165"
     CDS             1585..1953
                     /locus_tag="PKL26_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82728.1"
                     /translation="MITLLRKLYDVLEALKQKIGAAQDAASRLEEDIKHVQRKLGIIP
                     YTYDNWRLRNLLVPGLFIQEMWDRQVSDHVLDAVKYGMVGIKAKGTDKVELGRPPEIK
                     IVGPPEMYFGMDLVKDEKNG"
     gene            1946..2266
                     /locus_tag="PKL26_03170"
     CDS             1946..2266
                     /locus_tag="PKL26_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82729.1"
                     /translation="MGDEKVISLQAKREEKNTKEKGRTITGKARCLSCGHEWEHTAPE
                     GENGHYDLWHKCPSCGLQLGRFVYDVCENEPVWTCGGCGNELFKVTQKHIFCPVCGKI
                     QEFP"
     gene            complement(2397..2705)
                     /locus_tag="PKL26_03175"
     CDS             complement(2397..2705)
                     /locus_tag="PKL26_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82730.1"
                     /translation="MGKIVFPRTPICEVCKKEEAVSFSYLKNIPEANIKEGWYFCGYC
                     ISQDLVEDYYIEFDRFFHSPASTVDWLAHIAGKKWINWADFMAMMKRFREATGSFNQG
                     "
     gene            2856..3596
                     /locus_tag="PKL26_03180"
     CDS             2856..3596
                     /locus_tag="PKL26_03180"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82731.1"
                     /translation="MSRYCQMAGWNDAPHLTPEMKATIAAGIPPYQLKARSEGIPMLG
                     AGAIFPISEDSITCEPFTIPRTWPRAYGMDVGWNSTACAWGAWDTEGDILYLYNEYKV
                     GKEPPAIHTTAIKARGNWIPGVIDPHSDDSGQRDGAKLLEEYRDLGLDLQKANNSVEA
                     GLLEMWRRFTEGRLVVFSSLVAWFGEFRIYHRDENGKIVKVNDHLMDATRYLVMSGAD
                     VAISEATARAVIYRRAPRHQPGDMRMGY"
BASE COUNT          994 a         1009 c         1067 g          675 t
ORIGIN      
        1 gaaacccctg ataactccgg cgataaccca caaaggaaag gaaaggaaag gaaaggaaag
       61 gaaagcattg cggcgccggt aataactccg gaagaaaccg aagaaaaaga aggccctttt
      121 gaactgccct ccaaggaaga catcgtcgag tcgtcgcctt catggatcag ggacaagata
      181 gatgaagtct gcaaacgcct ggtccgggag aatatctatc cggaagcggt aaactacgtg
      241 ctcgcaaagc ggaatatgcg ggtgagcgag agagccctgt tgcatgcttt gtgcagggtg
      301 cacatctcaa aaccgaagca cccgaaagcg ttcatggaca agatcattgc catagaaaac
      361 gggaactaca atgaatacga gcattgtcag tcttcatgaa aagatcatag gggagttcat
      421 gtccctcgct gagaagatgg cagcggaggg gtgctcccaa caggacatta tgaaggcgtt
      481 caggaattat atcagcctaa aaacccgaaa gacgcacaaa aagcctccaa tgcagaaagc
      541 gagcagcgtc ataggggata tcgcgaagag caggcgggat gctgacagca acacggagcg
      601 aatcctacac gacatgatgg ttgaaagcgg gattcgcttc aagttccagt atcggatcgg
      661 tccgtatcgc gttgattacc tgattggaag cacgataatc gagctcgatg gaccgcacca
      721 caacgccaga aagctgcagg atcaggtgag agataagtac ctcagaaaac gcgggtacga
      781 gatcatacgg ataccaacat acatcttcaa cgtggccccg gatgcagtac ttgcggagat
      841 caaagggatg gagggcaaga ccccattctg atgcccatgt ccaaacgtga atataatccc
      901 atgagaaagg aggtgagtcc gcccgacacg atgtgagcca gaggggttta ccaaaatccg
      961 taatccagca gggggccgaa gggcccccgt atccaggagg gtgtatgaag aaaatcggag
     1021 tattgatcgc cataatgctc gcactcatca tcggctacag gcaaggccag gccgtggagt
     1081 tcgagtatga cggcagggtc gagtacaagt ttcggttctc agagaaagag atgcgcgaga
     1141 tcctaacgga acacctggtg gaaaaaggat acgaaatccc gcctgatggg gaggagtaca
     1201 tctcccacat cggcaccagg accatattca caatccacag gaagatcctc gcgccgccgg
     1261 ccattaaaat gccagagtat atccgaagcg aaaggggggc cgaaccgtga aaaaagccat
     1321 catcactatc ggaatcatcg taacgattct cgtcgtaagc cctgcctacg cgaacgacaa
     1381 gaagaccaag gacaccacgc cggcgctgcc caccaagcaa gagctcgtgt gggagaagaa
     1441 gtacctggaa gagcgattca acgctctgac agcaaacatc acgctctcgc agcaggaaat
     1501 atccgcgatc tctgtcaggt accaggaggt cgagcgcctg ctcaaggcga tcgaggagca
     1561 ggagaagccc aaggggaagg ggaagtgatc accctgctcc gcaagctcta tgacgtcctg
     1621 gaggccctga aacaaaagat cggggccgcc caggatgccg cctctcggct cgaagaagac
     1681 atcaagcatg tccagcgcaa gctggggatc atcccgtaca cgtacgataa ttggaggcta
     1741 cggaatctgc tcgtacccgg cctgttcatc caggagatgt gggacaggca ggtatcagac
     1801 cacgtgctcg atgctgtcaa gtacggcatg gtggggatca aggccaaggg caccgacaag
     1861 gtggagcttg gtaggccgcc agagatcaag atcgtcgggc ccccggagat gtacttcggg
     1921 atggatttgg taaaggatga gaagaatggg tgacgagaag gtcatctccc tgcaggcgaa
     1981 gcgggaagag aagaacacga aggagaaggg gcgcaccata actggtaagg cgcggtgcct
     2041 gtcctgcggg cacgagtggg agcataccgc cccagaggga gaaaacgggc actatgacct
     2101 ctggcacaag tgtccctcgt gcggcctgca gctcggcaga tttgtgtatg atgtttgtga
     2161 gaatgagccg gtttggacct gcggcggttg cgggaacgag ctcttcaagg taacacaaaa
     2221 gcatattttc tgcccggtgt gcgggaagat ccaggagttc ccgtgagtgt taaattcctg
     2281 ctcaccttac ccaccgtttc cgcctgtacg agaatcacgt tgcagggatc aagcgggttt
     2341 cctggaattt tccccgcaga cccctttttt aacaggagga tattatgagc ccggggctaa
     2401 ccctgattga agctacccgt tgcttccctg aatcttttca tcatagccat aaaatcagcc
     2461 caattgatcc acttctttcc tgctatgtgg gcgagccagt caactgtcga ggctggactg
     2521 tggaagaacc tatcaaattc aatatagtaa tcctccacta aatcctgaga gatacagtac
     2581 ccacaaaaat accaaccctc ttttatgttg gcttcgggga tgttcttaag atagctgaac
     2641 gaaacggcct cttccttctt gcacacctca cagatgggtg tcctcggaaa aacgattttt
     2701 cccactgacc acctccagaa tatgatttga ttttcaaaaa cgcacacctg aaaacccctg
     2761 tcaagtatca ataacgtgct ctaccctgtc aacggggtgt cagtaccctg tcaattccgg
     2821 tcacccccct aaaaaccggg gttaccatca cggccatgag caggtactgt cagatggccg
     2881 gatggaacga tgcaccgcat ctcaccccgg agatgaaagc gacgatcgcc gcgggcatcc
     2941 cgccctatca gctcaaggcg cgttcagagg gtattcccat gctcggcgcc ggcgcgattt
     3001 tccccatctc cgaggattcg atcacctgcg agccgttcac aatcccgcgc acctggccgc
     3061 gcgcctacgg catggacgtg ggctggaact cgaccgcctg cgcatggggg gcttgggaca
     3121 ccgagggaga catcctctac ctctacaacg agtacaaggt cggcaaggag ccgccagcta
     3181 tacacaccac tgcgatcaag gcgaggggca actggatccc gggcgtaatc gatccgcact
     3241 cggacgatag cgggcagcgc gacggcgcga agctcctgga ggagtaccgc gatctcgggc
     3301 tcgatctgca aaaggccaac aactccgtcg aggcgggact cctggagatg tggcggcgat
     3361 tcaccgaggg ccgcctggtg gtgttctcct ccctggtcgc atggttcggc gagttccgga
     3421 tctaccaccg ggacgaaaac gggaagatcg tcaaggtcaa cgaccacctg atggacgcca
     3481 cccgctacct cgtgatgagc ggcgcggacg tggccatctc cgaggcgacg gccagggcgg
     3541 tcatttacag gagggcgccc aggcaccagc cgggcgacat gagaatgggg tactgacatg
     3601 atggacaaca tcccacccac ctccaacgtg atcccgtttc caagcgcgac gaaccccgca
     3661 acgatggtgg tgcagaaccc atccaccccc ggcggcgaga tcctcatgca gcgaggccag
     3721 gaggtagcaa acggcctggc gcagc
//
LOCUS       DAOQVJ010000104         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27816_length_3740_cov_4.832564, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000104 DAOQVJ010000000
VERSION     DAOQVJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27816_length_3740_cov_4.832564"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..711
                     /locus_tag="PKL26_03185"
     CDS             370..711
                     /locus_tag="PKL26_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82732.1"
                     /translation="MNRTVLHIAEKAGYYARMGMETATTGNYTNALEYYDRALKEMPG
                     YAAIWREKGNCLDSMGRCDEAVRCFDQALQLDPGDAEAWFDKGLTLKKLGNEEEAFRC
                     MSRGVDLEIGV"
     gene            1075..1767
                     /locus_tag="PKL26_03190"
     CDS             1075..1767
                     /locus_tag="PKL26_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS82733.1"
                     /translation="MHESFYPRSIYPDARSVIVIGLPVHLPVLETTPSIYYHELYQTL
                     NRLLDEHTYQMAEWLNQEGYPSLFVPRDGYGSVEVLLKKPVAFFSHRHAAYLAGLGTF
                     GVNNMILTPGYGPRIRFGSVFTAAELPPDPVMEDDLCIHCMRCVEMCPSRALSGEEYP
                     EGITDKKACAEYSAGLFRRSISPCGICIKVCPVGADRAAFHREDADIYQKGHTPAGFA
                     SAWDHVRAYGGK"
     gene            1850..2080
                     /locus_tag="PKL26_03195"
     CDS             1850..2080
                     /locus_tag="PKL26_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNS82734.1"
                     /translation="MCIAVPAEVVEIKDGNIGVVDYGDLKQEVRLDLVDVKVGEFVLV
                     HVGFAIQKLSREEGLETREIFRQVYAAMQESE"
     assembly_gap    2117..2216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2391..2570
                     /locus_tag="PKL26_03200"
     CDS             2391..2570
                     /locus_tag="PKL26_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82735.1"
                     /translation="MDKTGVASLIAGIILVILGGYGVWIFLPDVIVFLKGVIGILAIL
                     IGIFLLIFGAILVKD"
     gene            2922..3626
                     /locus_tag="PKL26_03205"
     CDS             2922..3626
                     /locus_tag="PKL26_03205"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNS82736.1"
                     /translation="MNDTAVYENPGDENLSLRDILISMDDEGTLDEELLLTLLAEREW
                     NMSVGEEEIWVEPEPTPDPNAWKEYSNAKWRFSLIYPPTWEVKEGSSSSPAATFSAPV
                     EAECSSKTTECYQYVASLTVSIDQKPETIVLEDYFNKKVSKLQKDLAITATSKSAPTT
                     LSGNKAYWIEYYTRDSRGNPSKSYMQYFTILDKKVYILTYSGPFSTAENVYSRNKPEV
                     EKMIKEFVVEREYRTL"
BASE COUNT          888 a          894 c          983 g          875 t
ORIGIN      
        1 aagcctcctc gctgttttgt gtggggagac tgaatcgagg ttgttatctg catatcaatt
       61 cagtcactcc aaccgccgcg ggggcgcccc gtcgtgggcg gcggcgtgca tcgcatatgg
      121 gggtgtgggg gcggacagcc cccacaaaat gtgtccggtt caccaatcct acccacatca
      181 gatagcaaag agtttttttg atggaatgcc gtgcacttat gaattcgcac attgttgcag
      241 aggcgctgtg cactgcatac ctgcagagtt gatcggcaaa aatatacaat aaggggaatt
      301 ccaggattgt ctgtcggggg gggtgcactc tggacacccc ctgacccgga aaaaaggggg
      361 ttttacacca tgaacaggac agttctgcat attgcggaaa aagctggcta ttatgccagg
      421 atgggaatgg agactgcaac aacgggaaat tataccaatg cactggaata ctatgacagg
      481 gccctcaagg agatgcccgg ctacgcagcg atatggcggg aaaagggtaa ctgcctcgat
      541 tccatgggca ggtgcgatga ggcagtgcga tgcttcgatc aggccctgca gctggaccct
      601 ggcgatgcag aagcatggtt tgacaaggga ttgacactga agaaactcgg gaacgaggaa
      661 gaggcattca ggtgcatgag ccggggcgtt gatctggaga tcggtgtctg aagggattcc
      721 tcggtccagt gggggaggga tgatatatct gactctcccg gataattatt ctctctgcat
      781 tcgacaattt tttttccgag gataattcca tataccggtt tcgcagttca aatgttactc
      841 ctttcacatt atccgccagg atattacaac ccattgttcc gattattgga ttgaactgga
      901 agccccattg ggcccatatc caattgtttt aaatgagcca aacagatcac ctatgcgtgt
      961 tctatgaaca gcgaactcac acagacactt cgagaatggt gcagatgccg cgggataccg
     1021 gtgatggggg tggcgaacgc atccaggtgg gagaaaccgc cgttttcccc atggatgcat
     1081 gaatcgttct atccccggtc catatacccg gatgcccggt cagtgatcgt gatcggcctg
     1141 cccgtgcacc tgccggtcct cgagacaacc ccctccatct actaccacga actctaccag
     1201 acattgaacc gcctcctgga cgagcatacc taccagatgg cggaatggct gaaccaggag
     1261 gggtatccat ccctctttgt cccgagggat ggctacggaa gcgtcgaggt gttgctgaaa
     1321 aaacctgtcg cattcttctc gcaccgccat gccgcatatc tcgcggggct cggcacgttc
     1381 ggggtgaata acatgatcct tacacccgga tacggcccga gaatacggtt tgggtccgtc
     1441 tttacagcag ccgagctgcc gcctgacccg gtcatggagg atgatctctg catccattgc
     1501 atgcgctgtg tcgagatgtg cccctcgcgg gccttgtccg gggaagaata tccagagggt
     1561 atcactgaca agaaggcgtg tgcagagtac agtgccgggc tgtttcgcag atcgatctca
     1621 ccatgcggga tctgtatcaa ggtctgcccg gtgggagccg accgtgcagc atttcacagg
     1681 gaggacgcgg atatttatca aaaaggtcat acacccgcag ggtttgccag tgcgtgggat
     1741 catgtcaggg catatggagg gaaatgaccg ggcccttggg gaggttaccc aagagtacca
     1801 tttatgccct tgaaattcca ataatccatc aatattttgg gtgaaggtca tgtgtatcgc
     1861 tgttccggct gaagtggtgg agataaaaga tgggaatatc ggcgtcgttg attacggcga
     1921 tttaaaacag gaagtccggc tcgatctcgt cgatgtgaaa gtcggagagt ttgtcctggt
     1981 gcacgtggga tttgcaatac agaagctctc ccgcgaggag ggactcgaga cccgcgagat
     2041 cttcaggcag gtgtatgccg cgatgcagga atcggaataa ccatctcatc ctttttttgg
     2101 aattgagtat tcttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatg
     2221 caggaatcgg aataaccatc tcatcctttt tttggaattg agtattcttt ttagtgttac
     2281 aagacctcat tgcaccgcca cacaggcctc acagtctttc cgggaatcaa tacgcttatc
     2341 ttctaggaga tgtaaaaaat tgttgaaaaa gaggagagga cgtagtcaaa atggataaaa
     2401 caggtgttgc gagtcttatt gcgggaatca tccttgtaat tcttggtgga tatggggtat
     2461 ggatctttct ccccgacgtg attgtgtttc tcaagggtgt gatcggcatc cttgcgatcc
     2521 ttatcggtat attcctgttg atattcgggg ccatcctggt aaaagactaa aaagaggttc
     2581 gcacacggac acgttcgatt tccgggagcg ttcacgcttc cggcaatctc cctttctggt
     2641 aagatatcaa tctataaggc ctaacggctc ctaaattagt gcgttggggc gcagtatcgg
     2701 cggccttctc accatgaggt gcgactgtgg ataaagtgaa agttctcata gtgcttctgg
     2761 ccatctccct ttgcggaaac gtatttctca tattgagtgc cagcgagaac cctgtaaccg
     2821 gtgcagtcag caggtccatg atgcatgcct atcaccaggc ctttcctcca cttgataact
     2881 cctcggcgag tttgaattcc acagcgaatg cgacatatga gatgaacgat acggcggttt
     2941 atgaaaaccc gggggatgag aacctctccc tccgtgacat ccttatcagt atggatgacg
     3001 aggggacact ggacgaagag cttctcctca cgctccttgc ggagagggag tggaacatga
     3061 gtgttgggga ggaggagatt tgggtggaac ccgaaccgac tcccgatcct aatgcatgga
     3121 aggagtattc gaatgcgaag tggcgtttct ccctcatata cccccccaca tgggaggtca
     3181 aggaaggaag ttcgtcaagc cccgcggcga cattcagcgc acccgttgag gccgaatgca
     3241 gttccaagac caccgaatgc taccagtatg ttgcatccct gaccgtttcg atcgaccaga
     3301 agcctgagac tatcgtcctt gaggattatt tcaacaagaa ggtgtcgaag ctccagaaag
     3361 atctcgcgat cactgcgaca agcaagagtg ctcccacaac tctctccggc aacaaggctt
     3421 actggatcga gtattacacc cgggactcca ggggtaatcc atcaaagagc tacatgcagt
     3481 atttcacgat ccttgacaag aaagtgtata tcctgaccta tagcggcccc ttctcgactg
     3541 ccgagaatgt ctacagcagg aataagccag aagtcgagaa gatgataaaa gagttcgttg
     3601 tcgagaggga gtacagaacc ctctgaaacg cgttctccaa tttttcacga ttgtcccctt
     3661 tcgtgtaccc aaatccctat ggacaatttc tggttgcgtt cattctctcc ccccaagggt
     3721 ccaatagtcc cgcatcagac
//
LOCUS       DAOQVJ010000105         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27888_length_3735_cov_3.966304, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000105 DAOQVJ010000000
VERSION     DAOQVJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27888_length_3735_cov_3.966304"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..674)
                     /locus_tag="PKL26_03210"
     CDS             complement(249..674)
                     /locus_tag="PKL26_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82737.1"
                     /translation="MLCILSMVLPVVAANEEKYGYITIQNVDITLEKGKANIDVQYSL
                     DEPTRLIVLLLGKQDLKNRILKVLNYEEAEVVEIEMNRANLVVEDVSYNYGRGIYWFP
                     AHQFNVLIPTLRITTPQISREFCSTKMVPNGIGYFDNPE"
     gene            complement(949..1224)
                     /gene="albA"
                     /locus_tag="PKL26_03215"
     CDS             complement(949..1224)
                     /gene="albA"
                     /locus_tag="PKL26_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HNS82738.1"
                     /translation="MLKDNTVFVGNKPVMNYVLAVVTQFNNGAQEVSIKARGKAISRA
                     VDTAEIALNRFLEGVGKKEIITSTEIIDTDSGKTNVSSIEIVLSAPG"
     gene            complement(1237..2253)
                     /gene="asd"
                     /locus_tag="PKL26_03220"
     CDS             complement(1237..2253)
                     /gene="asd"
                     /locus_tag="PKL26_03220"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNS82739.1"
                     /translation="MINVGVLGATGAVGQRFVQLLAEHPWFNLTALTASDRSAGKQYG
                     EVVNWRLDVPFPKNVADIRIRTTDVKSLKDVDLVFSALPAEVAAGVETACADAGLGVC
                     SNASSHRMDPLVPLVVPEVNPDHLALIDAQRDGGRDGFIVTNPNCSTIVMCLSLAPLR
                     KFSFGDVRVATMQAISGAGFQGVPAMGIYDNVIPYIGSEEQKMETETLKILGHLDGKA
                     VKPASLKVSASCHRVPVIDGHTMAVWIDINEPVEMVRDAFREYVPPISGLPTQPERSL
                     LYLDDQDRPQPRLDRNRGNGMTVSVGRVREGIRYVTMGHNTIRGAAGASVLNAELIHT
                     RKYL"
     gene            complement(2299..2472)
                     /locus_tag="PKL26_03225"
     CDS             complement(2299..2472)
                     /locus_tag="PKL26_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS82740.1"
                     /translation="MTAVVDKEKCTGCETCVDECPAAAISMEDGKAKVDKDLCVDCGS
                     CVDVCPSEAIHLE"
     gene            complement(2546..3307)
                     /gene="dapB"
                     /locus_tag="PKL26_03230"
     CDS             complement(2546..3307)
                     /gene="dapB"
                     /locus_tag="PKL26_03230"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNS82741.1"
                     /translation="MTNVVVCGALGRMGTTIGRLVNEAPDLTLVGGVDVRAGDLFGTR
                     VVEAARMDALLKELKPDVVIDFTVANAAVENVRTAAKHGAALVVGTTGFSSEQRAEMA
                     RAVEGHVPAVISSNYSIGVNIFWQLLRQAARMLPDYDIEVLEAHHRYKKDAPSGTAKT
                     ILQILEEEVGEREKLYGREGMRERGREIGVHVIRGGDIVGDHAVIFAGNFETIQVSHR
                     AYDRSVFAQGALMAARWVVGKKPGIYGMNDVLGLA"
     gene            complement(3300..>3735)
                     /locus_tag="PKL26_03235"
     CDS             complement(3300..>3735)
                     /locus_tag="PKL26_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNS82742.1"
                     /translation="LVSELACHPNIVAIKEASGDIGQISRIIEGTLDEDFQVISGDDN
                     ITLPILALGGAGVISVAANVEPARMVAMFESFCDGDLDAALDMHFTLSPLFRAMFIDT
                     NPIPVKKAVELRGMAGGPPRLPLDELDDEKTVKLAEVLSTYD"
BASE COUNT          767 a         1126 c          976 g          866 t
ORIGIN      
        1 tccgtcacat cctgtccctt ccccttccat cctctcattg acggaggttt cccccggaaa
       61 gagcccctcg gatctctcac ctttccttta gaccatgtcg tcctgcgttg agatcgcaag
      121 gtatctggtt atgcatgccc accggaaaac cgaggaaaca cccggttttt ctaaatgtcg
      181 ggcagtggag atgaacgaaa tgccccccag cctccaatca ttcaggatga gaggcctcca
      241 gcctccaatc attcaggatt atcaaaatac ccgatgccat tcgggaccat cttggtagaa
      301 cagaattcgc gggatatctg gggggtggtg atgcggaggg tcggaattaa gacattgaac
      361 tggtgtgcgg ggaaccagta aatgcctctc ccgtagttgt aggaaacatc ctcgactact
      421 aggttcgccc ggttcatctc gatctccacc acctcggcct cttcgtagtt gagaaccttg
      481 aggatccggt tcttgaggtc ctgcttgccg aggaggagga cgatcaggcg cgtgggttcg
      541 tcgagcgagt actggacatc gatattggcc ttcccctttt ccagggtgat gtccacgttc
      601 tggatggtga tgtagccata tttttcctcg tttgccgcca ctacagggag caccatggac
      661 aggatgcaca acactgtaac tgcgatggct acgcctctca ttcgtgtagt atattagaac
      721 ctgccggtta tatcttttct ggtagtacct atccccccat ctgacagata tatcaagtca
      781 gaatcgaaaa caaattgatt aaagagtttt ttcatgcatg cactggaccg ggcaggtcat
      841 gactccgccc ctcaaaaagg aggagatttt tatggattcc ccgtgacatt ctacaccgcg
      901 gggtatcact gtcgcacgac ccatcctgac agactcgcat gagacggttt atcccggtgc
      961 cgaaaggacg atctcgatac ttgagacatt ggtcttgccg ctgtcggtgt cgatgatctc
     1021 cgtggaagtg atgatctcct tctttccgac cccttccagg aatcggttca gggcgatctc
     1081 tgctgtgtcc accgccctgg atatggcttt gccgcgtgcc ttgatcgaga cttcctgtgc
     1141 accgttgttg aactgggtca ctacagccag tacataattc atcaccggct tgttccccac
     1201 gaatactgtg ttgtctttta acattgagag gcaccttcag agatacttcc tggtatggat
     1261 caattctgca ttgagtaccg acgcacccgc tgcgcccctg atggtgttgt gccccatcgt
     1321 gacgtagcgg ataccctccc tcacccttcc gacgctgacc gtcatcccgt tgcccctgtt
     1381 ccggtcaagc cgtggctgcg ggcggtcctg gtcgtcaagg taaaggaggg atctttcagg
     1441 ctgggtcggg agtcccgaaa tgggggggac gtattccctg aatgcatccc tgaccatctc
     1501 caccggttcg ttgatgtcaa tccagactgc catcgtatgg ccgtctatca ccgggaccct
     1561 gtgacagctc gcacttacct tcagtgatgc aggtttcacg gctttcccat ccaggtggcc
     1621 aaggatcttg agagtctcgg tctccatctt ctgctcctcg gacccgatat agggaatcac
     1681 gttgtcgtag atacccatcg cggggacccc ctggaatcct gcgcctgata tggcctgcat
     1741 ggtcgccacc cggacgtccc cgaaggagaa tttcctcagg ggtgcaagag agaggcacat
     1801 gacgatggtg gagcaattgg ggttcgtcac gatgaacccg tccctccccc cgtcccgctg
     1861 ggcgtcgatc agtgcgagat gatcggggtt cacctcaggt acgacgaggg gaaccagcgg
     1921 atccatccgg tgagagcttg cattgctgca cacgccgagc cctgcatccg cacacgcggt
     1981 ctcaaccccc gccgcgacct ctgcaggcag ggcggagaat acaaggtcca cgtctttaag
     2041 gctcttcaca tcggttgtgc ggatcctgat atccgcgaca ttcttcggga acgggacgtc
     2101 gagtcgccag ttcacaacct ccccatactg cttccccgca ctccggtccg acgctgtcag
     2161 cgctgtcagg ttgaaccagg gatgctcagc caggagctgg acaaatcgct gaccaaccgc
     2221 tcccgtggct ccaagcactc cgacattgat catacgtaaa aaatagctgt atccctggag
     2281 ttattaaatc gtttgatttt attccagatg aatggcctct gacgggcata cgtccacgca
     2341 ggagccgcaa tcgacacaga ggtccttgtc aaccttggcc tttccgtcct ccatgctgat
     2401 ggctgctgcc gggcattcgt caacgcaggt ctcacaaccg gtacatttct ctttgtcaac
     2461 aactgctgtc atggttgcac gtatccagca taaaactgga taataaaaaa agaaataggt
     2521 ttctatttgg gtatttgcgc ccgtttcatg ccagccccag cacgtcgttc atgccataga
     2581 tcccgggctt cttcccgacc acccagcggg cggccatgag ggcgccctgt gcaaagaccg
     2641 agcggtcata tgcccggtgc gagacctgga tggtctcaaa attcccggca aaaatgactg
     2701 catggtcgcc gacaatgtct ccgccccgga tgacatggac gccgatctcg cgcccccgct
     2761 ccctcatgcc ctcgcgcccg tagagcttct cccgctcgcc cacctcctcc tcgaggatct
     2821 ggaggatggt ctttgcggtg ccgctgggag catccttctt gtagcggtga tgcgcctcaa
     2881 gcacctcgat gtcgtaatcg ggaagcatcc tggccgcctg cctgaggagc tgccagaaaa
     2941 tattcactcc gatgctgtaa ttgctcgaga tgacagccgg gacgtgcccc tcgacagccc
     3001 gggccatctc ggcacgctgc tcggaagaga aacccgtggt tcccaccaca agggctgcac
     3061 cgtgcttcgc ggcagtcctg acgttctcta ctgccgcatt tgcaacagtg aaatcaatga
     3121 ccacgtccgg cttcaattct ttgaggagcg catccatgcg ggctgcctcg acaaccctgg
     3181 tcccgaacag atcgcctgct ctgacatcca ccccgccgac aagggtaaga tcgggggcct
     3241 cgtttaccag cctgcctatt gttgtcccca tcctgcccag ggcaccgcac accacgacgt
     3301 tagtcatagg tggagaggac ctccgcgagt tttaccgtct tctcatcatc cagctcgtcc
     3361 agtggaagcc tcggtggacc tcctgccatg cccctgagct ccactgcctt cttcacgggg
     3421 atgggattgg tatcgatgaa catggccctg aagagaggcg aaagcgtgaa gtgcatgtcc
     3481 agcgcagcgt cgagatcacc gtcgcagaag gactcaaaca tcgccaccat acgtgcgggc
     3541 tcaacgtttg ccgcaactga gatgacgccg gccccgccaa gtgcgaggat cggaagggtg
     3601 atgttgtcat cccctgagat cacctggaag tcttcatcca gcgtcccctc aatgatcctc
     3661 gaaatctggc cgatgtcccc actggcctcc tttatggcga cgatgttggg gtggcatgca
     3721 agctctgaca cgaga
//
LOCUS       DAOQVJ010000106         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_27956_length_3730_cov_3.548027, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000106 DAOQVJ010000000
VERSION     DAOQVJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27956_length_3730_cov_3.548027"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKL26_03240"
     CDS             <1..269
                     /locus_tag="PKL26_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNS82743.1"
                     /translation="DPAGVMDSILAAVGEAYRLGVIHADLSEFNVMIDGNEVVLIDWP
                     QWISPDHPNAGEILARDVGNLVRYFSRKYRISTDLETAIERVTG"
     gene            266..2218
                     /locus_tag="PKL26_03245"
     CDS             266..2218
                     /locus_tag="PKL26_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HNS82744.1"
                     /translation="MKIFGIDVIKGSVRSRSRRPMYALVILHEREIVSESEVSGFRLL
                     RLLNAERPDILAVDSLQEIAVDQHDLFSFLQALPPSVRLVQVTGGERKETLGKVASRF
                     NISFNRFDPFDEARTIARVAALGAGAEVVAFENESEIVVSRHRSPGKGGWSQNRYVRK
                     MHGAVQAKAREIGMELMAAGLRYEKKETRAFGGCSRVSFTVRAPRDQIPVSTFRGADV
                     QVRISGKRLERIRFRPISSKPPYLIAGIDPGTTTAIAALDLDGTLLLLQSSRQISMSG
                     VIEALYRVGKPLIVASDVHDMPFSVEKIRRAFNAVAHTPRQDMSVEAKQTLTSGYSCQ
                     NDHERDALSAALEAYRTYRNKFQSLQKRVPPGYELEEVRAGIVRGQSVEQVIGDLNEK
                     AAPTEEKAPAVEMDAARDERIRILDGTIKRLRILVGELQEDLRERDLQVMRLQERLKR
                     VRSRQDQKVRADAEVTKRDVIIQNLKKRLRKEERTTRAMKRRVERMKEFDEALLSGET
                     IPLKILPALTREAIKTFSAEMGIREGDVLYVSRIEGFGKSVAKDLSQEGIAALLVGEF
                     AGKAVAMEVEAVFRELDIPLIAADQVRVVVKGGRGAADPGQFRDAVKRWKELQDRYEK
                     EKKGRMLEDIFREYRAERGKEMKKVG"
     gene            2211..3068
                     /gene="thiL"
                     /locus_tag="PKL26_03250"
     CDS             2211..3068
                     /gene="thiL"
                     /locus_tag="PKL26_03250"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNS82745.1"
                     /translation="MDERALKRVVMEVVGRDAVLDDCAILPLDDRFLVISTDMLHAST
                     DFPEQMTDWQVGWMSVAVTLSDIAAMGADPADILLAVGLDDPSRLRPILEGAKACCDA
                     YGARFAGGDLDSHRELTLVSSGTGFTRKPVRRAGSKVGDVVGIVGVPGRAQAALEGYP
                     KFQQYLFTPRPRVEEGRALAAAGATSMMDVSDGLLLSLFDMREANPCGYEIFTHKIPT
                     IEGIPQDLALDLALRGGGDFGLLFTCRPEVLPLPLPELYVIGNVIEEPAVMIDGKPAE
                     AKGYQHQWD"
     gene            complement(3070..>3730)
                     /locus_tag="PKL26_03255"
     CDS             complement(3070..>3730)
                     /locus_tag="PKL26_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82746.1"
                     /translation="ALLGAKDYQGAIDAYDQVLSIDPANSAAWAYKVTALQNLGRGEE
                     AVLITNNELKKDPDSLISLIKKAYALSALKRLDEALVIYNRVLERDPFNLDVLLQKGI
                     LQLDLGDYLGAIKTYDKVMEIDSNNTDALMYQGIAYYRSGQYERSIENYERMIALNPG
                     VSMAWSSRGYCLIKLGRIQDAIKSFDKALELYPGNSDAVYGRVEAIRVMWPSYTPAWD
                     R"
BASE COUNT          808 a         1018 c         1190 g          714 t
ORIGIN      
        1 cggatccggc aggtgtcatg gactccatac tggcagcggt gggggaggcg taccgcctcg
       61 gcgtcatcca tgcggacctc tcggagttca acgtgatgat cgacggcaac gaggtggtcc
      121 ttatcgactg gccgcagtgg atatcccccg atcacccgaa cgcgggggag atactcgcga
      181 gggacgtggg aaacctcgtc aggtacttct cgcgtaagta ccgtatcagc accgatctgg
      241 agactgccat tgagagagtg accggatgaa gatctttggc atcgatgtga tcaagggttc
      301 ggtgaggtcg aggtcgaggc gtcctatgta tgccctcgtg atcctgcacg aacgcgagat
      361 cgtcagcgag tccgaggtct cgggctttcg cctcctgcgc ctgctgaacg cggaacgccc
      421 tgatatcctt gccgttgaca gcctgcagga gatcgccgtg gaccagcacg acctcttctc
      481 gttcctccag gcactccccc cctcggtcag gttggtccag gtgacgggtg gcgagaggaa
      541 agagacgctc gggaaggtcg cgagcaggtt caacatcagc ttcaaccggt tcgacccctt
      601 tgacgaggcc aggacgattg cgagggtggc agcccttggg gcgggtgcag aggtcgtcgc
      661 gttcgagaac gagagcgaga tcgtggtcag caggcaccgc tctccaggaa aaggcgggtg
      721 gagccagaac cggtacgtcc ggaagatgca cggggcggtc caggcgaagg caagggagat
      781 cggaatggag ctgatggcgg caggcctccg gtacgagaag aaggagacgc gggcgtttgg
      841 agggtgcagc agggtctctt tcacagtccg ggctccaagg gaccagatcc cggtttccac
      901 gttccggggg gcggacgtcc aggtgcgcat ctcgggaaag aggctcgagc ggatacggtt
      961 ccgcccgatc tccagcaagc ccccctacct gattgcaggg atcgacccag ggacaaccac
     1021 ggccatcgca gccctcgacc tcgacggaac cctcctcctg ctgcagagct cccgccagat
     1081 ctccatgtcc ggggttatcg aggcgcttta ccgggtggga aagcccctga tcgtcgcctc
     1141 cgacgtgcat gatatgccgt tctcggtcga gaagatacga agggccttca acgcggtggc
     1201 acatacccca aggcaggaca tgagcgtgga ggcaaagcag actctcacgt caggatattc
     1261 ctgccagaac gaccacgagc gggatgcgct ctcggcggct cttgaggcat accggacgta
     1321 caggaacaag ttccagagcc tccagaagag ggtcccgcca gggtatgaac tcgaggaggt
     1381 gagggccggg atcgtccgcg gccagtcagt cgagcaggtg atcggggacc tgaatgagaa
     1441 ggcggcacca accgaagaga aggccccggc cgtcgagatg gatgctgcgc gtgacgagcg
     1501 gatccgtatc ctggacggga cgataaaaag gctccggatc cttgtcggcg aactccagga
     1561 agacctccgc gagagagacc tgcaggtgat gcggctccag gagaggctga agagagtgcg
     1621 ttcccggcag gaccagaagg tccgggcgga tgccgaggtg acaaagagag acgtaatcat
     1681 ccagaacctc aaaaaaaggc tccgcaagga ggagaggacc acccgtgcca tgaagagacg
     1741 cgtagagagg atgaaggagt ttgacgaagc ccttctgtcc ggagagacca ttccactcaa
     1801 gatcctgcct gcactcacaa gggaagcaat caaaaccttc tctgcggaga tgggaatccg
     1861 ggaaggggat gtcctctacg tgtcccgcat cgagggtttt gggaagagcg tcgcaaaaga
     1921 cctctcacag gaaggaattg cggccctgct tgtcggggag ttcgctggta aagccgttgc
     1981 catggaggtg gaggcagtat tccgcgaact ggatatcccg ctcattgcgg cagaccaggt
     2041 aagggtggtg gtcaaagggg gaaggggtgc cgcggacccc gggcagttca gggacgcagt
     2101 caagcggtgg aaggagttgc aggatcgcta cgaaaaggag aaaaaaggaa ggatgcttga
     2161 ggatatcttc agagaatatc gtgccgagcg gggaaaggag atgaagaagg ttggatgagc
     2221 gcgccctgaa gcgggtggtc atggaggtcg tgggcaggga cgcggttctc gacgactgcg
     2281 ccatcctccc cctcgatgat cggttcctcg tcatatcaac cgacatgctg catgcgagca
     2341 ccgattttcc tgaacaaatg accgactggc aggtgggatg gatgagcgtg gcagtgacgc
     2401 tctcggacat cgcggcaatg ggtgcggacc cggcagacat cctccttgcg gtgggactcg
     2461 acgacccatc gcggctacgc cccattctgg aaggggcaaa ggcttgctgt gatgcatacg
     2521 gcgctcgttt tgcgggggga gatcttgatt cccaccggga gctcaccctc gtcagctcgg
     2581 ggacagggtt cacccggaaa cccgtacgcc gggctggatc aaaggttggt gacgtggtcg
     2641 gaatcgtggg ggttcccggc agggcccagg cagcgctgga gggttatccc aagtttcagc
     2701 agtatctctt caccccacga ccgagggtgg aagagggacg ggcactcgcc gctgcaggtg
     2761 ccacctcgat gatggacgtg agcgacggcc ttctcctgtc cctctttgac atgcgggagg
     2821 caaacccctg cgggtacgag atcttcacgc acaagattcc tacaattgaa ggtatccccc
     2881 aggatcttgc actggatctt gctcttcgag ggggagggga cttcgggctc cttttcacct
     2941 gcagacctga agtccttccc ctgcccctcc ccgaactgta tgtaattggc aatgtcatcg
     3001 aagagcctgc cgtgatgata gacggcaagc ctgcggaggc caaggggtac cagcaccaat
     3061 gggattagat tatctgtccc atgcaggagt atacgagggc cacatcacgc gtatcgcttc
     3121 tacccggccg tacacagcat cgctattccc gggatataat tcgagtgcct tgtcaaatga
     3181 cttgattgcg tcctggatgc ggccgagctt gatgaggcaa tacccgcgac tagaccatgc
     3241 catggagacc cctggattga gtgcgatcat acgctcatag ttctctatcg atctctcata
     3301 ctgccctgac ctgtaatagg cgatgccctg gtacatgagt gcatcggtgt tgttggaatc
     3361 gatctccatc accttgtcat aagtctttat ggcaccgaga taatctccga gatcgagctg
     3421 gagtatcccc ttttgcaaca gcacatcaag attgaaggga tccctctcaa gcacccggtt
     3481 atagatgact aacgcctcgt cgagacgttt caatgcagag agtgcatagg ccttcttgat
     3541 cagcgaaatc aggctatccg gatctttctt gagttcgttg ttcgtgatga ggactgcctc
     3601 ttcccctctt cccaggttct gcagagcggt gaccttgtag gcccacgcgg cgctgttcgc
     3661 agggtctatc gagaggacct ggtcataggc atctattgca ccctggtaat cctttgcacc
     3721 caggagtgca
//
LOCUS       DAOQVJ010000107         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_28355_length_3693_cov_3.775151, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000107 DAOQVJ010000000
VERSION     DAOQVJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28355_length_3693_cov_3.775151"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..639)
                     /locus_tag="PKL26_03260"
     CDS             complement(235..639)
                     /locus_tag="PKL26_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82747.1"
                     /translation="MYLDELVKGRCPLCGCTLEDLEEVESEVDELLDRGDFSWLIFNY
                     FLFKQFTDLGASPLKVMQLVSLYEDTCATEDASRPDTRFELEITPGRFDRLVLRRCDR
                     CKKIFLGGGKKILSGDLALPGYRITYRCGSCV"
     gene            complement(1060..2055)
                     /locus_tag="PKL26_03265"
     CDS             complement(1060..2055)
                     /locus_tag="PKL26_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82748.1"
                     /translation="MEGTTETGENGQATADLPGEGAGAEPALLTREGMQLSRKGRHAE
                     ALDLFTQALTIAPTYIPAWVASGFALGKLGRYKEEIKACDQALALDPTLVDAWINRGF
                     ALGKLSRFSEKLVCCERAIVLNPSSAQAWNARGHTLGELGRYEEELTCTEIATSLRPR
                     YLGAWVNRGYALMKLNRFREAVSAYTRALEIFPGFAAAWVNMAIAYCHMEEFARAVSC
                     FYEAELLRPLENPRSLYWKGVALSRSGRRREAIAVLAKVVQDDPRQADTWIELSNCHF
                     MIGEIEESVRCFMIAYGIDKQDIKDNLSQAVTLLREGKKEEGVRCLSQVFGILLR"
     gene            complement(2182..3657)
                     /locus_tag="PKL26_03270"
     CDS             complement(2182..3657)
                     /locus_tag="PKL26_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNS82749.1"
                     /translation="MPPQDTPLVVARGTGSLGILPGMANRHGLIAGATGTGKTVSLRV
                     LAEQFSSIGVPVFMADVKGDLSGMARPGGDHAKVAERVQELGLSGFSYQGFPVVFWDL
                     FAEQGHPVRTTVSEMGPLLLSRILDLSDTQADVLTLVFRVADEQGLLLLDLKDLQAMV
                     RYAGEHAKELKTAYGNISPATIGAIQRSLLALDDQGGAKFFGEPSLEPAAFMKQSGGN
                     GTINILAADRLLQSPRLYSTFLLWLLSEIYEQLPEVGDPEKPALVFFFDEAHLLFADA
                     PKALLEKVVLVVRLIRSKGVGVYFVTQNPLDLPDDVLGQLGNRVQHALRAFTPKDQRA
                     VKAASETMRQNPGLDVKAAITELRTGEALVSFLDRKGSPGVTERAFIYPPMSRLAPLT
                     PGERDAVVQSSPFFGTYEKALDRASAYEMLASRAEASAVSQSKKEERETSQKRTRAPA
                     SAPRKAGTDMLTRMAGSAARAAGSQVGREIIRGILGSLAKK"
BASE COUNT          663 a         1157 c         1166 g          707 t
ORIGIN      
        1 cgcaactaga taggatcact cataccctga acccggccgc actccacaga cgatgacgct
       61 cctggggctg acgccccgcc gctgtggcaa ctccagaatg ctattgggcc agtgctgggg
      121 ggcatgagtg ataaaagaac tctgattcgg gagaaatcag ataatggatt ttgtaagagc
      181 ggtgtaagcc gacgggcgca agccctcgtt cggaaaaaag aagtcccgtt cccctcacac
      241 gcacgaaccg cagcggtagg tgatgcggta gccggggagg gctaaatcgc cggaaaggat
      301 cttctttccg ccgccgagaa agatcttttt gcaccggtcg caccggcgga gaacgaggcg
      361 gtcgaaccgc cccggggtta tctcaagttc gaaccgggtg tcggggcggc ttgcgtcctc
      421 cgttgcgcag gtgtcctcgt agagggaaac cagctgcatc accttgagcg ggcttgcccc
      481 gagatcggtg aactgcttga agaggaagta gttgaagatc agccaggaga agtcccccct
      541 gtccaggagt tcatccacct cgctctccac ttcttccaga tcttcaaggg tgcacccgca
      601 gagcgggcac cggcccttga caagctcgtc gaggtacacc tcctcctggc agcctgggca
      661 ggtggtatgg gcagcgtggg tacgggatgt catggttgaa tggtatggtc cggtattcgt
      721 tggtataaca gcgtttctgc gtgctgtagt gagctggtcc gcataacggt accttctatt
      781 caccgcacgc ctggatggtg agtatcaatg gcgcctgtgc accacgggtt cgacctccag
      841 gtaaattgcc tcttctattt gtacctctat cacataaacc atgcgctcgg ggacgtttcc
      901 tgcatggaat gggcgaacga aggatttttc gctggtgtat gaacaggttt tcgtgcgggc
      961 cgcaacctgg tgcagtcccc catgccgcaa agatgccgca gtgcgcctcg ggaggtgtgg
     1021 atatgcagac caatccttcc aggggtcaag gcctgcccgt cacctgagca ggattccgaa
     1081 cacctgggag aggcagcgca ccccttcctc cttcttcccc tcccggagaa gggtgaccgc
     1141 ctgggagagg ttgtccttta tgtcctgctt gtcgatcccg tacgcgatca tgaagcagcg
     1201 gacagattcc tcgatctccc cgatcatgaa gtggcagttg gagagctcga tccaggtatc
     1261 ggcctgtcgc gggtcatcct gcaccacctt tgcgaggacc gcgatggcct cacgcctccg
     1321 ccccgagcgc gagagggcca cgcccttcca gtaaaggctg cgggggttct cgagaggccg
     1381 gagcagttcg gcttcataga agcacgagac tgcccttgcg aactcctcca tgtggcagta
     1441 cgcgatagcc atgttgaccc atgccgcggc aaatcccgga aagatctcga gcgcccgcgt
     1501 gtacgcggaa accgcttccc tgaaccggtt cagcttcatc agcgcgtagc cccggttcac
     1561 ccacgcaccc aggtaccggg ggcgaagcga cgttgcgatc tcggtgcagg tcagctcctc
     1621 ctcgtaccgc ccgagctcgc cgagggtgtg gcctcttgcg ttccaggcct gcgccgaaga
     1681 ggggttcagg acaatggcac gctcgcagca gaccagtttc tccgagaagc gggagagctt
     1741 tccgagcgca aagccacggt taatccaggc atcgacaagt gtcgggtcaa gggcgagcgc
     1801 ctggtcgcag gccttgatct cctccttgta cctccccagt tttccgagcg caaaacccga
     1861 tgcaacccag gcgggaatgt acgtcggggc tatggtgagc gcctgtgtga agaggtcgag
     1921 ggcttcggcg tgccgcccct tccgggagag ttgcatcccc tctctcgtga ggagtgcagg
     1981 ctccgctccc gccccctcac cagggaggtc ggcagtcgcc tggccgttct ctcctgtctc
     2041 tgtcgtccct tccatgccca tgcctgcgaa gccctgtatt ccactattgt ataccctgac
     2101 cgggctgcat aaatgcatcc atgacgacat gcacccttcc ggggaaccag ccgggcgctt
     2161 gaggaccagg agcaggcagg ctcacttctt cgcaagcgac ccgaggatcc cccgtatgat
     2221 ctcccttccg acctggctcc ccgcggccct cgcggcgctc cccgccatcc tggtcagcat
     2281 gtcggtgccg gccttccgcg gggcggacgc gggtgcccgg gtcctctttt gggaggtctc
     2341 ccgctcctct tttttggatt gggacacggc ggaagcctcg gccctcgaag ccagcatctc
     2401 gtacgcggag gccctgtcca gggccttttc gtaggtccca aagaaaggtg acgactggac
     2461 gacggcatcc cgctcgccgg gggtcagggg ggcgaggcgg ctcattggcg ggtagatgaa
     2521 ggcccgctcg gtcacacccg gggagccctt ccggtcaagg aatgagacga gcgcctcgcc
     2581 ggtccgaagt tccgtgatcg cggccttcac atccaggccc gggttctgcc gcatggtctc
     2641 cgaggcagcc ttcactgccc tctggtcctt cggggtgaac gcccgcagcg cgtgctggac
     2701 acggttcccg agctgcccga gcacgtcgtc cgggaggtcg agggggttct gggtcacgaa
     2761 gtagaccccc accccctttg acctgatcag cctgaccacg aggacgacct tctcgaggag
     2821 cgccttcggc gcgtcggcga agaggaggtg cgcctcgtcg aagaagaaga caagggccgg
     2881 cttctcgggg tcccccacct cggggagctg ctcgtagatc tcggagagga gccagaggag
     2941 gaaggtcgaa tagagccggg gagactggag gagcctgtcg gcggcgagga tgttgattgt
     3001 gccgtttccg ccggactgtt tcatgaacgc cgccggttct aacgacggct cgccgaagaa
     3061 cttcgccccg ccctgatcgt cgagcgcgag caggctccgc tggatggcgc cgatggtggc
     3121 cggcgagatg ttgccgtacg cggtcttcag ctcctttgcg tgctcccccg cgtacctgac
     3181 catggcctgg aggtctttta ggtcgaggag aaggagcccc tgctcgtcag cgacccggaa
     3241 gaccagcgtg aggacgtcgg cctgggtatc agacaggtcg aggatccgcg agaggagcag
     3301 cggccccatc tccgagacgg tggtccgcac cgggtggccc tgctccgcga agaggtccca
     3361 gaacacgacc gggaagccct ggtacgaaaa ccccgagagc ccgagttcct gcacccgctc
     3421 ggcaaccttc gcgtggtcac cgccgggcct ggccatcccc gagaggtccc ccttgacgtc
     3481 ggccatgaag accggcacgc cgatagagga gaactgttct gcgagcacgc ggaggctgac
     3541 agtctttcct gtccccgtgg ctcccgcgat cagcccgtgg cggttggcca ttcctgggag
     3601 gatgcccagc gacccggttc cccgggccac gacgagaggt gtatcctgtg gcggcatatg
     3661 tgatgtaaat cccctgtatt cttcatgggg gag
//
LOCUS       DAOQVJ010000108         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_28448_length_3685_cov_3.905510, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000108 DAOQVJ010000000
VERSION     DAOQVJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28448_length_3685_cov_3.905510"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..495)
                     /locus_tag="PKL26_03275"
     CDS             complement(64..495)
                     /locus_tag="PKL26_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HNS82750.1"
                     /translation="MKREARAIIHCRGHPHVRAAHPSTLEITCEHEMTPNGDCIIAVG
                     ADCSARDLPEEFRILLCRDDASLHTVFSCGGMSVEVRGQGSSSMTLDHPADMVWRRSC
                     YVCGRTVAIGSDTTARMMPREFVEALRKGGEVVATLTVRVP"
     gene            complement(641..1480)
                     /locus_tag="PKL26_03280"
     CDS             complement(641..1480)
                     /locus_tag="PKL26_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82751.1"
                     /translation="MVLDSLKRACGLLLKNPFLWLPGLAAGALGAADLLLQYYLGAFL
                     ATRLWFIEALLIPFLLAGAYVQIRSGEKGAVAFVAGATAYYFRILLPSLVIVFAVLAT
                     VFLVVIPLTFMGLAGTALPFVVMGCTVPIIFFTFFFDTAAVFEDRRVFDCIRRSVEVV
                     MNRPGRVIAFYLVCLGIVVLVAIPLAVIWTGLLYEQLLPLATMDPSQVQAFSVSMFNE
                     MLGQEGILITAIFYLVAMMVAGNIICTFKAVFFRDTSDEIPADASPVQQGEFDEKGRW
                     YKY"
     gene            complement(1521..2075)
                     /locus_tag="PKL26_03285"
     CDS             complement(1521..2075)
                     /locus_tag="PKL26_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNS82752.1"
                     /translation="MSFVERNCYYFDAPGPANTVDCARFALERARELSIERVVVASTS
                     GDTALAFYRMLRGSGIKLVVVTHVVGFSRPGEWEFSPEAARTLKLEKIPIVTGTHVLS
                     GMERAFSRSPKVGGGSRTEAVAEALRRVISVGLKVAVECTLIAADQGVIPVDKEVIAV
                     GGTASGADTVCVVRPSHTASFFDL"
     gene            2119..3288
                     /locus_tag="PKL26_03290"
     CDS             2119..3288
                     /locus_tag="PKL26_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNS82753.1"
                     /translation="MITLALAGKPNCGKSTFFRAATMAHAEIANYPFTTIDANFGVAY
                     VRTECPCKSLGLQCGNCADGIRFVPVNLIDVAGLVPDAHKGKGLGNQFLDHLRQADAI
                     IHILDASGSTDTEGNPVETGSHDPREDIAFLQKEMTMWVYGILEKHWARLQRQAQSKA
                     YSIHRGISEVFTGLGITPEDVQDAERSVAIDLIRAEPDGLVKFCAEIVAISKPMIPVG
                     NRVDMAPAPLLDTLRSAGITFATAAGELALRNAVSGGLISYHPGDPAFSIIKEESLSP
                     AQKAGLQTIAAVMKANGGTGVQQAIDRAVYELLGMIVVYPVEDEHKFSDSRGRILPDA
                     FLMKRGSTPRDLAFQVHTDIGKAFLYAVDARTKMRVKDTHELKPGDIIRIVSAAK"
     gene            3372..>3685
                     /locus_tag="PKL26_03295"
     CDS             3372..>3685
                     /locus_tag="PKL26_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82754.1"
                     /translation="MGGDVFQAQILRNFFDTITGTDRNLTRIYMCVVSLAKIRMEPPE
                     KFTFLLEQLRKSKQHKELSVDILDYMCDVAAQLEIAAVQTAFGVKEVGEIQQEFGSIS
                     MDS"
BASE COUNT          847 a         1098 c         1062 g          678 t
ORIGIN      
        1 actcctggca tccgcggaga tgaagatcac gatgctcggg agggactaac ctccctgtga
       61 ctctcagggg accctgaccg tgagggtggc cacaacctcg cctccctttc ggagagcctc
      121 tacgaactcc cggggcatca tgcgggcggt agtgtcagaa ccaatcgcca cggtcctgcc
      181 gcacacgtag cagctccttc tccataccat gtcggccggg tggtcaagtg tcattgaaga
      241 ggacccctgc ccccggacct ccacgctcat ccccccgcat gagaagactg tatgaagaga
      301 ggcgtcatcc ctgcagagga ggatgcggaa ctcctccggg aggtcgcgtg cactgcaatc
      361 agcaccaacc gcgataatac agtccccatt gggggtcatt tcgtgctcgc aggttatctc
      421 gagcgtggag ggatgagccg ccctcacatg cggatgaccc ctgcaatgga taatggccct
      481 ggcttccctt ttcattcctg aataatggta tatgaaagaa gagtaaagag atgatgcatt
      541 gggagtgtca ggctcccgtg acggcacaac ccctaaaaag gggcattcct ttttttttga
      601 ctcacgcatg ccccatatct cccttttttc gaactttttt ttagtatttg taccagcgcc
      661 ctttctcgtc gaattcaccc tgttggactg gggaggcgtc cgcgggaatc tcatctgaag
      721 tatcgcggaa gaagactgcc ttgaaagtac agatgatatt tcccgcgacc atcattgcaa
      781 caaggtagaa tatcgcggta atgaggatcc cttcctgccc aagcatctcg ttgaacatgg
      841 agacagagaa tgcctggacc tgggaggggt ccatggtcgc aagcgggagg agctgttcgt
      901 agagaagccc cgtccagatc accgccaggg gtattgccac cagcaccacg atgccgaggc
      961 acaccaggta aaatgcaatc accctcccgg gccggttcat caccacctcc acgctccggc
     1021 ggatacagtc aaagaccctg cggtcctcga agactgccgc ggtatcaaag aagaatgtga
     1081 agaagatgat gggaactgta catcccatga ccacaaacgg gagggcggtc ccggccaggc
     1141 ccatgaacgt aaggggtatt acgaccagaa agactgttgc aagcacggcg aacacgatca
     1201 cgagcgaggg gaggaggatc cggaaataat aggctgtcgc cccggcaaca aaggctactg
     1261 cgcccttctc gcccgagcgt atctggacgt atgcgccggc aaggaggaac gggatgagga
     1321 gggcctcgat aaaccagagc cgtgttgcaa ggaatgcacc cagataatac tgcagcagga
     1381 gatccgccgc accgagcgca cccgccgcga ggccagggag ccagaggaat gggtttttca
     1441 acagcaggcc acacgcacgc ttcagcgagt cgaggaccat ccgttcacct gttccttggc
     1501 atcgccacga tctcgcgaac ctacaggtca aagaacgagg cggtgtgtga cgggcggacc
     1561 acgcagaccg tatctgctcc gcttgccgtt cccccgaccg cgattacctc cttgtcgaca
     1621 ggaatgaccc cctggtcggc ggcaatcagc gtgcactcga ctgcgacctt caggccgacc
     1681 gagatcacac gccgaagtgc ctctgcgacg gcctccgtcc gggagcctcc gcccaccttc
     1741 ggggagcggg agaacgcacg ctccatcccg gaaaggacat gcgtcccggt cacaatgggg
     1801 atcttctcaa gcttcagggt ccgggcagcc tcgggcgaga actcccattc accaggccgc
     1861 gagaacccca cgacgtgcgt gacgaccacc agcttgatgc ctgagccccg gagcatccgg
     1921 taaaacgcaa gggccgtgtc tcccgaagtg ctggccacca caaccctctc aattgacagt
     1981 tcacgcgccc tttcaagggc aaacctggca cagtccacgg tgttggcagg gccgggcgca
     2041 tcaaaataat aacagttcct ctcgacaaac gacataggca ccctaaggtt aaggtggtac
     2101 caggtaaagg gaattgctat gatcacgctc gcactcgccg ggaagccaaa ctgcggcaag
     2161 tcgacctttt tccgggcggc gacgatggca cacgccgaga ttgcaaatta cccattcacc
     2221 accatcgacg cgaacttcgg agtggcctac gtccgcaccg aatgcccttg caaaagcctc
     2281 gggctccagt gcggcaactg tgccgatggc atcaggttcg tgccggtgaa cctgatcgac
     2341 gtggctggcc ttgtcccgga tgcccacaag ggaaagggcc tcgggaacca gttcctggac
     2401 cacctgcgcc aggcggacgc aatcatccac atcctcgacg cgagcgggtc cacggacacc
     2461 gaggggaatc ccgtcgagac aggcagccac gacccgcgag aagatatcgc attcctccag
     2521 aaggagatga caatgtgggt atacgggatc ctggagaagc actgggcaag gctccagcgc
     2581 caggcccagt caaaggcgta ttcgatccac aggggcatct ccgaggtctt cacgggcctt
     2641 ggcatcacgc cggaggatgt ccaggacgcg gagaggagtg ttgcgatcga cctgatccgg
     2701 gcagaacctg acggtcttgt aaagttctgt gccgagattg ttgcgatcag caagcccatg
     2761 atccctgtcg ggaaccgggt tgacatggcc cctgctcccc tgctggacac gctccgctcc
     2821 gcagggatca catttgccac cgcggcaggg gagctcgcgc tccggaatgc cgtgtcgggc
     2881 ggcctgatct cctatcaccc cggcgacccc gcgttctcaa taatcaaaga ggaatccctc
     2941 tcccctgccc agaaagcagg tctccagaca atcgctgcag tcatgaaggc caacgggggg
     3001 acgggggttc agcaggccat cgacagggca gtctatgaac tgctcgggat gatcgttgtc
     3061 taccccgtcg aggacgagca caagttctcg gactcgcgcg gccggattct ccctgacgcg
     3121 ttcctgatga agagaggctc gaccccgagg gaccttgcat tccaggtcca taccgatatc
     3181 ggaaaggcgt tcctctacgc tgtcgacgca aggacgaaga tgagggtgaa ggacacccac
     3241 gaactcaaac ccggcgatat cattcgcatc gtgagtgccg cgaaatgacc gcttttttgg
     3301 ggaatacggg gagaaaaccg ttatttctga gatagtttta tatagtggca attgccaacc
     3361 ggagtattag catgggtggc gacgtattcc aggcccagat attgaggaat ttcttcgata
     3421 ccatcacggg taccgaccgg aacctcaccc gcatttacat gtgcgtggtg agccttgcca
     3481 agatccggat ggagccccca gaaaaattta ctttcctgct cgaacagctg cggaagagca
     3541 agcagcacaa ggagctctcg gtggatatcc ttgactacat gtgtgacgtg gctgcccagc
     3601 tcgagatcgc ggcggtccag accgcgttcg gggtaaaaga ggtcggggag atccagcagg
     3661 agttcggaag catcagcatg gactc
//
LOCUS       DAOQVJ010000109         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_28575_length_3673_cov_3.906025, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000109 DAOQVJ010000000
VERSION     DAOQVJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28575_length_3673_cov_3.906025"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1501
                     /locus_tag="PKL26_03300"
     CDS             266..1501
                     /locus_tag="PKL26_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HNS82755.1"
                     /translation="MHDVTIPSVNIGVVGHVDHGKTTLVYGLTESWTDRHSEEIKRGI
                     SIRLGYADATFYRCERCEGAEAFGTRPECPVCGGKAVPFRSVSFVDAPGHETLMATML
                     SGSALMDGAMLVIAANETCPQPQTKEHLMALELVGIKKIVIVQNKIDVVPQKEAVKHY
                     EQIKSFVKGTIAENAPIIPVSAQKVINMGALVVALDQTIPIPDRDPGAEPMMLLARSF
                     DINKPGSSWRDLKGGVIGGSLIRGVLKEGEDIEIRPGRQVQAENRIRWEPIETKITTI
                     NAGSKKVVEATPGGLLGIGTKLDPALTKSDALAGQVAGHPGKLPPVWEKARFRVTLME
                     RVVGVSSEMQIEPLKHHEPLMLSVGTAVTVGVVINTKKDIVEVTLKRPVCAEIGARIA
                     ISRQLGGRWRLIGMGVLTE"
     gene            1498..1878
                     /locus_tag="PKL26_03305"
     CDS             1498..1878
                     /locus_tag="PKL26_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNS82756.1"
                     /translation="MKVLVDANALMVPFQFRIDIFEELRNLIGAYEPLILTEVLQELG
                     GLSRGRGRGGSAARSALLLSRRCLETASGYPEGTVDEKIARYAGEHGCMVFTNDRALR
                     DTLLELGIPVITLMQQKKLDILRR"
     gene            1895..2488
                     /locus_tag="PKL26_03310"
     CDS             1895..2488
                     /locus_tag="PKL26_03310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HNS82757.1"
                     /translation="MYYKMQLADKVRVPPNRLGEDLTRVILDVLQEHLEGSIDKEIGI
                     FISITKVLRIGEGEIVPGDGAVYYDVDFEAVVLRLALQEVLEGLVVETTSFGAFVSLG
                     PIDAMLHVSQISDEYINYDEKNARLICQESKRFIGVGEPVRVRVVTLSLNEREPRDSK
                     IGLTMRQSGLGTARWLEEDMLKEKEKEKEKGGERGSR"
     gene            2475..2678
                     /gene="spt4"
                     /locus_tag="PKL26_03315"
     CDS             2475..2678
                     /gene="spt4"
                     /locus_tag="PKL26_03315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HNS82758.1"
                     /translation="MAAVRKKLVHVCRECHRVVEGEACAVCGSSNLSTDWTGYLVIID
                     PKRSEVARKMNITLPGRYALKVR"
     gene            2678..3172
                     /locus_tag="PKL26_03320"
     CDS             2678..3172
                     /locus_tag="PKL26_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HNS82759.1"
                     /translation="MLRLPESQRHLFKSPFGTLVPEIADLADVLPGRRLYTVGDVVTR
                     NVLEMGLLPEVAVVDGHTMREPCSRAPEVFPAVFPAKNPPGTITPMLVEALKKAVANP
                     PALVVVEGEEDLAVIPLVFEAPEGAWILYGQPCKGVVVREIDEEARATAKSLLACFIE
                     EPEE"
     gene            3249..3545
                     /locus_tag="PKL26_03325"
     CDS             3249..3545
                     /locus_tag="PKL26_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HNS82760.1"
                     /translation="MEFEVTRDTRNELLGRREVDFTLQFDGPTPSRKQILGKISALMN
                     VPEKQVVVDSLKTSFGMTACTGSARVYDSEERRNQIERAYLMNRGMPKPKEEGA"
     gene            3548..>3673
                     /locus_tag="PKL26_03330"
     CDS             3548..>3673
                     /locus_tag="PKL26_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HNS82761.1"
                     /translation="MAAKKKAKAASVKRGSYFKVEGGKVSFSRKYCPRCGPGVILA"
BASE COUNT          836 a          983 c         1194 g          660 t
ORIGIN      
        1 gcccgaagag acgggtcgat cgtcgtgcgt gaccctctgc gggattcggg catactccgc
       61 tatcctccat actaccggag cgtgcgcctc ccggaagagg aaaggggcac cccctgatcc
      121 cgtcttcttt tgggcacatc ccggcaggag ctgcgccccg gccactctgc agaccggaga
      181 aaaaccacac cttcgcgaaa aaatccctgc tcgcagggta ttaagtagga ttgttcttat
      241 atagatcagc gaggtttgat cggcattgca tgacgttaca attcccagcg tgaatatcgg
      301 cgtagtcggg cacgtggacc atggaaagac caccctggtg tatggcctca ccgagtcctg
      361 gacagaccgg cacagcgagg agatcaagcg gggaatctcc atccgcctgg gatatgcaga
      421 tgccacattc taccggtgcg aacgctgtga aggcgcagaa gccttcggca ccaggccgga
      481 gtgccccgta tgcggcggca aggcggtacc gttccggtcg gtctccttcg tggatgcacc
      541 gggccacgag acgctgatgg ccacgatgct gtcggggtct gcattgatgg acggggcgat
      601 gctcgtgatc gcggcgaacg agacctgtcc acagccccag acgaaagagc acctgatggc
      661 gctcgaactg gtggggataa agaagatcgt gatcgtccag aacaagatcg acgtggtccc
      721 acagaaggag gcagtcaagc actacgagca gatcaagagc ttcgtgaagg ggacgatcgc
      781 cgagaacgca ccgatcatac cggtctctgc acagaaggtg atcaacatgg gggcgctcgt
      841 cgtggccctc gaccagacca tccccatccc ggaccgtgat cccggtgcag aacccatgat
      901 gctcctcgca cggtccttcg acatcaacaa gcccggctca agctggcgcg acctcaaggg
      961 cggggtgata ggtggctcgc tgatacgggg agtgttaaaa gagggggagg atatcgagat
     1021 aaggccgggg aggcaggtcc aggccgagaa caggattcgc tgggagccga tcgagaccaa
     1081 gatcaccacc atcaatgccg gttcgaagaa ggtggtagaa gcaacgcctg gcgggctgct
     1141 tgggatcggg acgaagcttg accccgcgtt gacaaagagc gacgccctcg caggtcaggt
     1201 tgccggccac cccggaaagt tgccgcccgt ttgggagaag gcgcggttca gggtcacgct
     1261 gatggagcgg gtggtgggcg tgagcagcga gatgcagatc gagcccctca agcaccacga
     1321 acccctcatg ctctcggtgg gaactgcggt caccgtcgga gtcgtgatca acaccaagaa
     1381 ggacatagtg gaggtcaccc tcaagcggcc ggtctgcgcg gagatcgggg cccgtattgc
     1441 catcagccgc cagctgggag gaagatggcg tctgatcggg atgggcgtgc tgaccgagtg
     1501 aaggtcctcg tcgacgcaaa tgccctgatg gtccccttcc agttcaggat tgatatattc
     1561 gaagagctga gaaaccttat tggggcatat gaaccattga tactgacaga agtgctccag
     1621 gagctgggag ggctctcccg gggccgggga agagggggaa gtgctgcccg ttctgccctc
     1681 ctcctttccc ggcgatgcct ggagactgcg agcggatatc cggaagggac cgtcgatgag
     1741 aagatcgcac ggtacgcggg ggagcatggg tgcatggtat tcaccaacga ccgggctctc
     1801 cgcgatacgc tccttgaact tggcataccg gtgatcaccc ttatgcagca gaaaaaactt
     1861 gatatattga ggagatagcg ggagagagcg gcgcatgtat tacaagatgc aactggcgga
     1921 caaggtacgg gtacctccca accggcttgg tgaggacctc acgagagtga tcctggacgt
     1981 gctgcaggag cacctggaag ggagcatcga caaggagata gggattttta tctccatcac
     2041 gaaggtgctg aggatcggag aaggagagat cgttcccggg gacggcgcgg tctattacga
     2101 cgtggacttc gaggcggtgg tgctccgcct ggctctccag gaggtgctcg agggcctggt
     2161 ggtggagacc accagttttg gtgcgttcgt gagcctcggg cccatcgacg ccatgctcca
     2221 cgtgagccag atctccgacg aatacatcaa ctacgacgag aagaacgccc gtcttatctg
     2281 ccaggaatcc aagcggttca tcggcgtcgg cgaacctgtc agggtaaggg tggtcaccct
     2341 ctccctgaac gagcgggagc cgagggacag caaaatcggc ctgacaatgc gccagtcggg
     2401 cctcggaacc gcgcgctggc tcgaggaaga catgctcaag gagaaggaga aagagaagga
     2461 gaagggtggc gagcgtggca gccgttagga agaaactggt ccatgtatgc cgtgaatgcc
     2521 acagggtggt ggaaggcgag gcctgtgcgg tatgcggcag ttccaacctc tccacggact
     2581 ggacagggta tcttgtgatc atcgacccca agcgatccga ggtggccagg aagatgaata
     2641 tcacccttcc cggccggtac gcgctgaagg tccgctgatg ctccgtcttc ccgagagcca
     2701 gcgccacctt ttcaagtcac cttttggcac gctcgtgccg gagattgccg atcttgccga
     2761 cgtacttccg ggaaggcgcc tttatactgt cggcgacgtg gtgaccagga acgtgctgga
     2821 gatgggcctc ctccccgagg ttgccgttgt agacggccac acgatgaggg aaccctgcag
     2881 ccgtgccccc gaagtctttc ctgcggtgtt tcctgcaaag aacccacccg ggaccatcac
     2941 ccccatgctg gtcgaggcat tgaagaaggc agttgccaat ccgcccgcgc tggtggtggt
     3001 cgagggagag gaagacctgg cggtcattcc ccttgtcttc gaggcaccgg aaggggcatg
     3061 gattctctat ggacagccgt gcaagggagt cgtcgtaagg gagatagacg aggaggcacg
     3121 ggcgacggca aaatctcttc ttgcctgttt tatcgaagaa cctgaagaat gaggggagat
     3181 ttttcctaat cgatccgaca acgagataga atataaataa ttttactgcg tatgataaga
     3241 ggataccgat ggaatttgag gtcaccaggg ataccaggaa cgagctgctc gggaggcgtg
     3301 aagtcgactt tacactccag ttcgacggcc ccacaccttc caggaagcag atcctgggca
     3361 agatttccgc actgatgaac gtgccagaga aacaggtggt ggtcgactcg ctgaagacga
     3421 gtttcgggat gacggcctgc acggggagcg caagggtata tgacagcgag gagaggcgca
     3481 accagattga gcgcgcatat ctcatgaacc gcggaatgcc caagcccaag gaagagggtg
     3541 cctgaagatg gcggcaaaga agaaggcaaa ggcagcctct gtcaagaggg ggagttactt
     3601 caaagtggag ggggggaaag tctcgttctc caggaagtac tgccccaggt gcggccccgg
     3661 cgtgatcctt gcc
//
LOCUS       DAOQVJ010000110         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_28588_length_3672_cov_4.799281, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000110 DAOQVJ010000000
VERSION     DAOQVJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28588_length_3672_cov_4.799281"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="PKL26_03335"
     CDS             <1..1725
                     /locus_tag="PKL26_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNS82762.1"
                     /translation="FEIIVGGKTDPAFGKVITLGTGGTLVEFLRDVSIRVLPIDREGV
                     SDMIDETRGSRIIQGFRGSPPLDKGALIDTVLRLAHLFEHNSDWHEFDINPLILYPHG
                     CVAVDARIYLSVRSEKEGIVQIPSPDPAIFSPRSVAVIGASSDPRKIGYVVLRNLLQF
                     PGPVYPVNPKSPEILGKTAYSRVTEIPGDLDAAVIAIPAPAVPGVLRQCTEKGVKLAI
                     ILSSGFRESGDEGAALEKEILEIARAGEMRIVGPNCLGIIVPHANLNTTFDPVSPMNG
                     HIGFISQSGAVVTTIVDWSIPEYIGFSVVISVGNQVDLGFVDFLRVVAADPHTRAIIL
                     YIEEIRRGREFLSAVKEITAKKPVIALKSGSSRKGQNAAASHTGSLAGNYQVYQAAFR
                     QTGIIPVYTIQEAFDVAELLASEGYPKGNRALVITTAGGFAVMASDYAEWYGIALPPL
                     TDEMVTALDEFLPPMWSRENPLDIIGDGGAERYARVFDVLTRFQDEWDIAVVIAVPSA
                     ILDPSHLAQEIARFSRNTHKMVIGCMLGGDTMRSGERVLRERHIPNYREIDGAFRAVG
                     RALSARPQ"
     gene            complement(1755..>2321)
                     /locus_tag="PKL26_03340"
     CDS             complement(1755..>2321)
                     /locus_tag="PKL26_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNS82763.1"
                     /translation="SGDDTPFIIFTGKGNETVAMEACNAGADYYLTKSGSPREIFYRL
                     KECVMRASWGYLLRHPWDIAFTTPLGVLETLPMPVFVLDERLHIRYLNRSATRISGLK
                     THDLEPFSFNGLLEKNSSRELIVFLEHVIGGSESQDDATCPVPLVFMGRDGRCIHMEC
                     SILPARDENGRPTGFVVTCKESMLRDPG"
     assembly_gap    2322..2421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3022..>3672)
                     /locus_tag="PKL26_03345"
     CDS             complement(3022..>3672)
                     /locus_tag="PKL26_03345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNS82764.1"
                     /translation="GLNHENIVRVSGMNILPIPYVEMEYIPKTLSDLPKPLPPRSAAR
                     ICRDVARGLLFAHERGIIHRDIKPQNILVTYEGVPKITDWGMSKVMGVTGMPTITGFS
                     LAYAAPEQLAPQTYGETDQRTDIYQLGCVLYELITGTVPFPGNDMVQVSAHILSETPR
                     LPSTMNPDALPLDSIIMRCLEKSPENRYQDVRDLIRDLDAYLLSAETSDRYNIFED"
BASE COUNT          802 a          985 c         1000 g          785 t
ORIGIN      
        1 ttcgagatca ttgtgggggg gaaaacagat cctgcctttg ggaaggtgat cacgttaggc
       61 acggggggca ccctcgtcga gttcctccgc gatgtctcca tcagggtgct tcccatcgac
      121 agggaaggcg tttcggatat gatcgatgag acccgggggt cccgtatcat acagggtttc
      181 cgaggatctc cacccctgga caaaggagca cttatcgaca ctgtccttcg ccttgcacac
      241 ctcttcgagc acaattccga ctggcacgag ttcgatatca accccctgat actgtacccc
      301 cacggttgcg tggcagtgga tgcccgcatc tacctgtccg tccgatcaga aaaggaaggt
      361 attgtgcaaa tcccttcccc ggacccggca atcttttccc cacgctccgt tgccgtcatc
      421 ggcgcctcat ccgatccgcg aaagattgga tatgtggtcc tgcgaaacct cctccagttc
      481 cccggacctg tgtaccctgt caatccgaaa agcccggaga tactcgggaa gacggcatac
      541 tcccgcgtca cggagattcc cggcgacctg gatgctgcgg tcatcgccat cccggctccc
      601 gcggtcccag gggtacttcg ccaatgtaca gagaaagggg tgaagctagc catcatcctc
      661 tcatcaggat tccgtgagag tggagatgaa ggcgcagcgc tcgagaagga gatcctggag
      721 atcgcaaggg caggggaaat gcgtatcgtg ggcccaaatt gccttggaat cattgttcca
      781 cacgcaaacc tcaacaccac ttttgatcct gtctcaccta tgaacggcca tatcgggttc
      841 atctcgcaga gcggggcggt cgtcaccacc atcgtggact ggagcatccc tgagtatatc
      901 ggcttttccg tcgtaatcag cgtgggcaac caggtcgatc tcgggttcgt cgactttctc
      961 cgcgtggtgg ctgccgatcc acatacccgg gcgatcatcc tctacatcga agagatacga
     1021 cgcgggagag agttcctctc tgcggtgaag gagattaccg cgaaaaaacc ggtcattgcg
     1081 ctcaagtcag gatcttcgag aaaggggcag aatgctgccg cctcgcacac cgggtcactc
     1141 gcaggaaatt accaggtgta ccaggcagcg ttccgccaga ccgggattat cccggtgtac
     1201 accatccagg aagcgttcga cgtggccgaa ctcctggcct ctgaaggata cccgaagggg
     1261 aacagggccc tcgtgatcac caccgcgggt ggctttgccg tgatggcgtc tgattatgca
     1321 gagtggtacg gcatcgcact tccacctctc acggatgaga tggtaacggc actggatgag
     1381 ttcctccccc caatgtggag ccgggagaac ccgctcgaca tcatcggcga cggtggggcg
     1441 gagaggtatg caagggtctt tgatgtgttg acccgtttcc aggacgaatg ggacattgcg
     1501 gtggtgattg cggttccatc agccatcctt gatccctccc acctggcgca ggagattgcc
     1561 cgcttctcca ggaacacaca caagatggtg atcggttgca tgctcggggg cgacaccatg
     1621 aggagtggag aacgagtgtt gagagagcgg catatcccaa attaccgcga aattgacggg
     1681 gcgttcaggg cagtggggag agcgctctct gcacgtccgc aataaaagaa atgacttcgg
     1741 gatccccaac tggactaccc gggatcccgc aacatgctct ccttgcaggt cacgacgaat
     1801 ccggtgggac ggccgttctc gtcgcgggcc gggagaatac tacattccat gtggatacat
     1861 ctgccatccc tgcccatgaa tacaagggga accgggcacg tggcatcatc ctggctttcg
     1921 ctgcctccta tcacatgttc gagaaaaaca atcaattccc tggacgaatt tttctcgaga
     1981 agaccgttga aggaaaaggg ctccagatca tgggttttca atccggagat cctggtggca
     2041 gaccggttca ggtagcggat atgcagcctc tcatcaagaa cgaagaccgg catgggaagg
     2101 gtttcgagca ctccgagcgg ggtcgtgaat gcgatatccc aggggtgtcg tagaaggtat
     2161 ccccaggatg cgcgcatcac gcattccttc aggcggtaaa atatttcccg cggggaaccc
     2221 gacttggtga ggtaatagtc agctccggca ttgcatgctt ccatcgctac cgtctcattt
     2281 ccctttcctg tgaagatgat gaacggcgta tcgtcacctg annnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn natgtgtcca atttaaatga agaaccgcgg cactatggtg
     2461 tggtgaggca tggaagtgag gcagggggac agccaccccg aagcaggggc gaccggtgac
     2521 gggataggtt gtggcggtga aaggaatcat gttcatcccg ctcgccggcg aggcacgacg
     2581 cccgtcccta atgtgccgcg gagtcccccc tccccgctgt cccttgcgag gaggcatggg
     2641 acagcatgca acaccacccc gaatcatcca ccaatctgtc agaggaatgc acacatcatt
     2701 gaggcatcac accatatcgg caattggaca cttgtagcgc atgccagatc ctgaaaaact
     2761 tgcacacaca ctcacaatcg atgcagaact ctcccggtaa gccctgtaaa gggccgggag
     2821 agtatcaata cttttctcca aaaaaactac atgattccct ttgagacaca cgcatacgca
     2881 ttcatgaaaa tggcgcagcc attttcatag taaaaaacgg cttcgctgtt taccatggac
     2941 tacacatgtg taattcatgg atggaaatct tggggtttat tttttaaatt gcggttcctg
     3001 cgatagcgac cgatacaggt ttcaatcttc gaatatgttg tagcggtcac tggtttcagc
     3061 actgagaagg tatgcatcaa ggtctctgat aaggtccctt acatcctgat agcgattttc
     3121 cggactcttc tcgaggcagc gcatgataat cgaatcgagc ggcagtgcat ctggattcat
     3181 ggttgacggg agtctgggtg tttccgagag gatgtgggca ctgacctgca ccatgtcatt
     3241 gcccgggaac ggaacggtcc cggtgatcaa ttcatacaac acgcacccca actggtatat
     3301 atcggtgcgc tggtcggtct ctccatatgt ctggggtgcc agttgttcgg gtgccgcgta
     3361 ggcgagagaa aaaccagtga tggtgggcat tccagtcacc cccatcacct tgctcatccc
     3421 ccagtccgtg atcttcggta ctccctcata ggtcaccagg atgttctggg gcttgatatc
     3481 ccggtggata atccctctct cgtgtgcgaa gaggaggccc ctggcaacgt cgcgacagat
     3541 ccgtgcggca gatcttggcg gaagaggttt tggcagatcg gagagggtct ttgggatgta
     3601 ctccatctcc acatacggga tgggcaggat attcatgccg ctcactctca cgatgttctc
     3661 gtgattcagt cc
//
LOCUS       DAOQVJ010000111         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_28589_length_3672_cov_4.373514, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000111 DAOQVJ010000000
VERSION     DAOQVJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28589_length_3672_cov_4.373514"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..393
                     /locus_tag="PKL26_03350"
     CDS             73..393
                     /locus_tag="PKL26_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82765.1"
                     /translation="MQNAGTLSGGEQQMLAIGRCLMADPEMIVLDEPSLGLSPKLVHM
                     VLGTVQRLNDEGLTILLVEQNVRRALEISHRAYVLESGRITRTGPADDLLHDDQIREA
                     YLGM"
     gene            complement(629..1594)
                     /locus_tag="PKL26_03355"
     CDS             complement(629..1594)
                     /locus_tag="PKL26_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNS82766.1"
                     /translation="MKIFDPRDYRHLLAAALLVIAFLIPIFIGSNLYYLTILILMLVF
                     IIYASAWNFLTFSGQGSLGHAAFFGLGGYAASLVAIRFGLPPLVTLLFGGCFAAFIGI
                     LIGLTCVRLREWFLAMVTFGFAVIIQTLIVSQLAPITGGWDGFPVPKLVPTNIPGYLM
                     IEYYLILFIAIGVILIFRYLLQSRIGLALAAIRENEVEARAAGINPVPFKLFAFGVSA
                     FVTGIAGGLEIFHFGYITPEIFGIDLSFWPIIYSITGGLGTLAGPVIGTIVVTLLWDG
                     LNAVGLTYARFIIIGVLLVLIIIFLPRGLVSLPEEIQKRIRQKKT"
     gene            complement(1591..2472)
                     /locus_tag="PKL26_03360"
     CDS             complement(1591..2472)
                     /locus_tag="PKL26_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNS82767.1"
                     /translation="MDGTIGIISQVAFWGLYSGCIYILLATGLNLIFGVMKIVNFAHG
                     EFLMLGTYITFFLFVISGFNPYVLLIGSIPILIIVGVIVERLCFRPILGTGKLNEIFI
                     SLGLIYIFQNLVAMIWTDEWRSMKSPFADILVSLGPLQLPLDYLIIITVTALILVSLY
                     LFLQKTPLGRAIRAASQNRKGAMLMGVNVERMDMLTFGIGAGLAGAAGTLWAVSGQVF
                     NPYMGSIPAIKGFAIIILGGLGSIPGAIIGGLAIGMVENFTAFTLGGSWKDAVSFILL
                     IVVLVIRPTGLFGEAEE"
     gene            complement(2506..>3672)
                     /locus_tag="PKL26_03365"
     CDS             complement(2506..>3672)
                     /locus_tag="PKL26_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS82768.1"
                     /translation="DIWQSAELAADEINAKGGIYVKELGKSVPIRLVQGDDESTREGG
                     QKAVSKLITQDNVDVLVGGYSSAVVSAHQSIVAEYKVPYIISGGSSTSVSHRDDIDTS
                     YMFFHRPNTDDSGEASILFVDEVVRPSILTKFGFSSDRPLKMAVIYQDSPFGKGQLDA
                     VKNVIKARNLPIEIVAEEPFKMGESDFRTVLTSVKAKNPDMIYSVTFLNELVPLTQQA
                     RRDVGLNTLILAIENNDDPDYYKGVGTYGDGQVMESRFSPYAVPRGSTEEATRQFLTN
                     FDKKYGGFAGMMGAATYESVYITKAAIEDAGSIDKAKVRDALATLSMPQMMEIMKGGT
                     ISFSPDYRESKFDLYVVQLAMDQASGEPRPHIVWPENLKDKEFVLPDWYTPGSS"
BASE COUNT          841 a          996 c         1062 g          773 t
ORIGIN      
        1 ggatatgtcc gggagaatgg caacgcgctt gaccatgtct tctagctctt tccagtcctt
       61 aaagataggc aggtgcagaa tgctgggact ctgagcggag gggagcagca gatgctcgcc
      121 attgggagat gcctgatggc ggacccggag atgatcgtcc tcgatgagcc gtcacttggc
      181 ctctccccga aactcgtgca catggtgctt ggaaccgtgc agcgcctcaa cgacgaaggc
      241 ctgaccattc tcctcgtgga gcagaacgtg cgcagggcgc ttgagatctc tcaccgggcg
      301 tacgtcctcg aaagcggaag gatcacgaga accggacccg ctgacgatct tctccacgat
      361 gaccagataa gggaagcgta tctcgggatg taaatccata ccccgaagct ctttttcgtg
      421 tcatatgcaa gagggatgat gccatccccc taatgagtct tggtggtcca gtgcaggtgt
      481 aaatgcgacc ccttgcccca aaaagcactt ttttttggtt cctggtcggc agcggtcgcc
      541 catatcagga atgggagggc cagggatcgt ggcgtttgga cccctggaat agccaggacc
      601 ggcattaagt gagattggtg tgacccaatc aggtcttttt ctgcctgatc ctcttctgta
      661 tttcctccgg gagcgagaca agtccccttg ggaggaatat gatgataagc acaaggagga
      721 cgccaatgat gatgaaacgg gcatacgtga ggccaaccgc attgaggccg tcccagagca
      781 gcgtcaccac gattgtgccg atgaccggtc ccgcgagagt tccaagcccc ccggtgattg
      841 aatagatgat gggccagaac gagaggtcga ttccgaatat ctcaggggtg atatacccga
      901 agtgaaatat ttcgaggcca cctgcaatcc ctgtgacgaa tgcactgacg ccgaacgcga
      961 agagtttgaa ggggaccggg ttgatcccgg cggcccttgc ctcgacttca ttctcccgta
     1021 tcgcagcgag ggcaagccct atcctcgact ggagcaggta ccggaagata aggatgaccc
     1081 cgattgcgat gaaaaggatc aggtaatact cgatcatcag gtaccctgga atattggttg
     1141 gcacgagctt tggaaccggg aacccgtccc agcccccggt gattggggct agctggctca
     1201 cgatgagcgt ctggatgatc accgcaaaac cgaatgtcac cattgcgagg aaccattccc
     1261 gcaggcggac gcacgtgagg ccgatgagga tcccgatgaa ggccgcgaag catcctccaa
     1321 agagcagggt gacgagtggc gggaggccga accggatagc gacaagggat gcagcgtacc
     1381 ctccgaggcc gaagaaggct gcatgcccga gcgacccctg gccagagaag gtgaggaagt
     1441 tccaggcaga cgcgtagatg atgaagacca gcatcaggat gaggatagtc agataataga
     1501 ggttcgatcc gatgaagatg gggatgagaa aagctatcac caggagggcc gcggcgagca
     1561 ggtggcggta gtcccgtgga tcgaatattt tcattcttca gcctccccaa agagcccggt
     1621 aggccggatg acgaggacga caatcaggag aatgaaggat accgcatcct tccaggagcc
     1681 gccgagggtg aacgcggtga agttctccac catcccgatt gcaaggcccc cgatgatggc
     1741 gcccgggata ctcccgagcc cgccgaggat gatgatggca aagcccttga tggccgggat
     1801 cgagcccatg taggggttga agacctgccc gctcaccgcc cagagggtgc ctgccgcacc
     1861 ggcaaggccg gcaccaatcc cgaaggtgag catgtccatc ctctccacgt tgacccccat
     1921 cagcatggcg cccttgcggt tctggctcgc ggccctgatt gcccgcccga gcggggtctt
     1981 ctggaggaag aggtagagcg agacaaggat cagcgcggtg accgtgataa tgatcagata
     2041 atcaaggggc aattggaggg gacccaggct gaccaggata tctgcaaacg ggctcttcat
     2101 tgacctccat tcgtcggtcc agatcatcgc caccaggttc tggaatatgt aaatcagccc
     2161 aaggcttatg aatatctcgt tcagcttccc cgtgccgaga atcggccgga agcacaaccg
     2221 ctccacgatg actcccacga tgatgagtat gggaatggaa ccgatgagga ggacatacgg
     2281 gttgaaccca ctgataacaa agaggaagaa tgtgatatac gttccaagca tcaggaactc
     2341 gccatgggca aaattcacga tcttcatcac accgaagata aggttgaggc cggtggcaag
     2401 caggatgtag atgcatcccg aataaaggcc ccagaaggca acctggctga ttatcccgat
     2461 ggtcccgtcc atacagtaat ccttctcctg tgcctaaaaa aagatttagg aggatcctgg
     2521 ggtgtaccag tcaggcagga caaattcttt gtcttttagg ttttcgggcc agacgatgtg
     2581 gggccggggt tctccagacg cctggtccat tgcaagctga acgacgtaga gatcgaactt
     2641 gctctcccgg taatccggtg agaaggagat ggtgccaccc ttcatgatct ccatcatctg
     2701 tggcatggaa agtgttgcga gtgcatccct tacctttgcc ttgtcgatgc tcccggcatc
     2761 ctcgatggct gcttttgtga tatatactga ctcgtaggtc gcggcaccca tcatccctgc
     2821 aaacccgcca tattttttgt cgaaattggt gaggaactgc cttgtggcct cttcagtcga
     2881 gccccttggc actgcgtacg gcgagaacct gctctccatc acctggccgt cgccatatgt
     2941 gcccacgccc ttgtagtaat cggggtcgtc attgttctcg atggcaagga tcagtgtgtt
     3001 gagaccgaca tctctccttg cctgctgggt caggggcacg agttcgttca agaatgtcac
     3061 ggagtagatc atgtcaggat tctttgcctt cacggaggtc aatactgtcc tgaagtccga
     3121 ctcacccatc ttgaacggtt cttccgcaac gatctcgatt gggaggttcc tggccttgat
     3181 cacgttcttc accgcatcaa gctgcccctt cccgaacggg ctgtcctggt agatgacggc
     3241 catcttcagt ggacggtccg aggagaaacc gaacttggtg agtatggacg gcctgacaac
     3301 ttcatcgacg aacagtatgc tcgcctcgcc ggaatcgtct gtgttcgggc ggtggaagaa
     3361 catgtagctg gtatcgatat catcgcggtg cgagacggat gtgctcgaac ctcccgatat
     3421 gatatatggc accttgtact ctgcgacaat agactggtgg gcagagacca cggcactcga
     3481 atagccccca acgaggacat ccacgttatc ctgggttatc agcttggaga ccgctttctg
     3541 gccgccctcg cgggtggatt cgtcatctcc ctgtacaagg cgtatcggaa ccgatttccc
     3601 gagttccttc acgtatatgc cgcccttcgc gttgatctca tccgcggcca gttctgcgga
     3661 ctgccagata tc
//
LOCUS       DAOQVJ010000112         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29108_length_3626_cov_4.046486, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000112 DAOQVJ010000000
VERSION     DAOQVJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29108_length_3626_cov_4.046486"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PKL26_03370"
     CDS             complement(<1..1488)
                     /locus_tag="PKL26_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS82769.1"
                     /translation="MHITELEIDNFKSFTKKTKIPFYEGFSVISGPNGSGKSNIIDSI
                     LFVLALSGSRTLRAEKLTDLINLNSGRHTAEVTLAFSDGTRIRRRIKRTSSGYYSYIY
                     LNNRLSKQVEVTDFLAKHGIKPHGYNVVMQGDITRIIEMSDFERRKIIDEIAGVAEFD
                     QKKGQALIELEVVRERIEREELLLRELSQRLEELAAEREQALKFRHWQERLQHLEGLR
                     AAATLHAREKDLAALSRVIEDERLQLDRVVSDRSIEENEREYLKKDLADIDSQINQKS
                     GAEYLRLIAELEEAKGTIKLAEQTISRLKKEKEAGLEGINRTYIDQKRAETRVSECTQ
                     AIREMNIDRTNLAMEAATAAAQKEKIEQQIQSQSRDAEGARDELFALMQSIEEKKNER
                     SSLLHQKDMLIEKSRMRTSERERMEERLKALEAEGAGKEQRLSENEREMEALTQEKAE
                     LDRTLSELESSVFARRTTLERVREEYRSHEQDVVRLEAQQQARGEA"
     gene            complement(1503..1982)
                     /locus_tag="PKL26_03375"
     CDS             complement(1503..1982)
                     /locus_tag="PKL26_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82770.1"
                     /translation="MEIIKDKDRSLAPVDERVAALEKKMREMEAMVKGLTEELLDLKS
                     VAMKLSKASEERSRAEMRMSKPVAPAASSPIILQKKSVSAPAMQRPAQAPVETAPEKM
                     DMIMQPNGTLAPEKRKNSDYIIASAGFSKKKQSAAGPGKKNDLIVAEDEETDSAHKK"
     gene            2301..3539
                     /gene="glyA"
                     /locus_tag="PKL26_03380"
     CDS             2301..3539
                     /gene="glyA"
                     /locus_tag="PKL26_03380"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNS82771.1"
                     /translation="MSNLATTDPEIADIVRKEMERQRDGLELIASENMVSRAVLEAMG
                     SVMTNKYAEGYPGKRYYGGCEFYDMAENLARDRLKELFGAEHANVQPHSGTQANMAVY
                     FATMKLGDLMLSMKLSQGGHLSHGSPVSFTGQFYRVAQYGVDPKTEILDYGVVEEMAR
                     KERPAMLVCGASAYPRTIDFRAFQEIADGVDAYCMADIAHIAGLVAAGLHPTSVGVTD
                     FTTTTTHKTLRGPRGGAIMCNSEYAQIVDKAVFPGMQGGPLMHTITAKAVCFKEALRP
                     SFKEYNRQIIRNSKALAETLHEKNLRLVSGGTDNHLILLDLSDLGITGLEAEVALGRA
                     HITVNKNTIPNEQRSPFVTSGLRVGTPAVTSRGMKEEEMRQIGEWIGTILRDIHNEGA
                     IRRIGEEVVSMAGKFPLYPE"
BASE COUNT          693 a         1091 c          974 g          868 t
ORIGIN      
        1 cgcctcaccg cgggcctgct gctgtgcctc cagccgtact acatcctgtt cgtgggaccg
       61 atactcctcc ctgaccctct cgagggtggt acggcgggca aacacgctgc tctccagttc
      121 cgagagggta cggtcaagct ccgccttctc ctgagtgagg gcttccatct cgcgttcgtt
      181 ctcggaaagc cgctgctcct tccctgctcc ctcggcttca agcgccttca gccgctcctc
      241 catcctctcc ctctccgatg tgcgcatccg gctcttctcg atgagcatgt ccttctggtg
      301 gagaagcgag gagcgttcgt tcttcttctc ctcgatgctc tgcatcaggg cgaagagctc
      361 gtccctggcc ccctccgcat ccctgctctg cgactggatc tgctgctcaa tcttttcttt
      421 ctgcgcagca gccgtcgctg cctccattgc aaggttcgtg cggtcgatgt tcatctcccg
      481 gattgcctgg gtgcattcgg atacccgtgt ctcggcccgt ttctggtcta tataggtcct
      541 gttgatcccc tcaagcccgg cctccttctc cttcttcaac ctggaaatgg tctgctccgc
      601 gagctttatc gtccccttgg cctcctcgag ctccgcgatc aggcgcagat attctgcccc
      661 gctcttctgg ttgatctggg aatcgatatc tgcaaggtcc ttctttaggt attcgcgctc
      721 gttctcctcg atgctacggt cggagacgac ccgatcgagc tgcaaccgtt catcctcgat
      781 caccctggag agtgcggcaa ggtccttctc cctcgcgtgc agggttgcag ctgccctgag
      841 cccctccagg tgctggagcc gctcctgcca gtgcctgaac ttcaatgcct gttcccgctc
      901 tgccgcaagc tcttcaaggc gctgcgagag ctcccgaagc aggagctcct cgcgttctat
      961 ccgttccctc accacctcga gctcgatgag cgcctgcccc tttttctggt cgaattcagc
     1021 cactcctgcg atctcatcga tgatcttgcg gcgctcgaag tcactcatct cgatgatccg
     1081 ggtgatatcg ccctgcatca ccacgttgta cccgtgcggc ttgatgccgt gctttgcgag
     1141 gaaatctgtc acctcgacct gcttggagag ccggttattg aggtaaatat aactgtaata
     1201 cccgcttgac gtgcgcttta tacgccggcg tattctggtc ccgtccgaga acgcgagtgt
     1261 gacctccgcg gtatggcggc ccgagttcag gtttatcagg tctgtcagct tctcggcacg
     1321 gagagtccgc gaccccgaaa gggcaaggac aaaaaggata ctgtcgatga tgttgctctt
     1381 tcctgatcca tttggccctg atatgacaga aaaaccctcg taaaacggga tcttggtctt
     1441 ttttgtgaaa gatttgaaat tatcaatctc aagctcggtg atatgcaaag accaaggccc
     1501 cgttatttct tgtgcgcaga atccgtctcc tcatcttcgg ccacgatgag gtcgttcttt
     1561 tttccagggc ccgcggcact ctgcttcttt ttggagaaac cggcagatgc aatgatgtaa
     1621 tcactgttct tccgcttctc gggcgcaaga gtcccgttcg gctgcataat catgtccatc
     1681 ttctcgggag ccgtctctac cggtgcctgc gccggccgct gcatcgcagg tgcggagacg
     1741 gatttcttct ggaggattat agggctgctt gcggcgggtg ctaccggctt gctcatccgc
     1801 atctccgccc gcgaccgctc ttcactcgcc ttcgagagct tcatggccac ggatttgagg
     1861 tccagcagct cctcggtgag ccccttgacc atggcctcca tctccctcat cttcttctcc
     1921 agggccgcga cccgttcgtc aacgggagca aggctccggt ccttgtcctt tattatctcc
     1981 atatctgact cctcggggct ggtccgcctc tcatacctgt ggtggggcgg tgggtggcat
     2041 tcaggagagc cctcattgat tatcatgaat tattccccta taataattat gctgccagtt
     2101 gcggcggttt ttttcaaatt tctctcgctt aatcccactc tcatggagtt gcaaccgaac
     2161 accccggtat tccggaacgt ttttttaaac cctcgatccg gcgcattttc cagtctaact
     2221 gtgagaagag ttgaaatcct tgagtttttt tttcactgga gaagaaaagg tattacctga
     2281 tcagatcaac ctttttatac atgtccaatc tggccaccac ggatccggaa atagcagata
     2341 tagtcaggaa agagatggaa cgccagcgtg acggcctgga gttgattgcc tccgagaaca
     2401 tggtcagccg tgcagtgctc gaggccatgg gctctgtcat gaccaacaag tatgcggagg
     2461 gttacccggg gaaacggtac tatggagggt gcgagttcta cgacatggca gagaacctcg
     2521 cacgggaccg gctcaaggaa cttttcgggg cagagcatgc gaacgtgcag ccacactcag
     2581 gcacccaggc aaacatggcg gtctattttg ccaccatgaa actcggggac ctcatgctct
     2641 ccatgaaact ctcccagggg ggccatctct cccatgggtc acctgtcagc ttcacgggtc
     2701 agttctaccg ggttgcccag tatggagtag accccaaaac agagatactg gactacgggg
     2761 tggtagagga gatggcgcgg aaggagcgcc cggccatgct ggtgtgcggg gccagcgcat
     2821 acccgagaac catcgatttc cgggcattcc aggagatcgc ggatggagta gatgcctact
     2881 gcatggccga tatcgcccat attgcagggc ttgtcgccgc cgggctccac ccgacatctg
     2941 tcggggtgac agattttacc actacaacca cccataaaac gctccgtgga ccccgcgggg
     3001 gcgctatcat gtgcaattcc gagtatgccc agatagtgga caaggcggtc ttcccgggta
     3061 tgcagggcgg ccccctgatg cataccatca ccgcaaaagc cgtctgcttc aaggaggcgc
     3121 tccggccctc ttttaaggaa tataaccggc agattatcag gaactcaaaa gccctcgccg
     3181 aaacgctcca tgagaagaac ctccggttgg tttcaggcgg taccgacaat cacctcatcc
     3241 tccttgactt gagcgacctc ggcatcaccg gccttgaagc tgaggttgcg ctcgggcggg
     3301 cacatatcac ggtgaacaag aacaccattc cgaacgagca gcgcagcccc ttcgtgacga
     3361 gcggcctccg ggttggcact cctgccgtga ccagcagggg catgaaggag gaagagatgc
     3421 gtcagatcgg ggaatggatc gggacaatcc tccgtgacat ccacaacgag ggggctataa
     3481 ggaggatcgg ggaggaggtt gtgtccatgg ccggaaagtt ccccctctac ccggagtgac
     3541 atgatcctcg atgggaagaa ggtgtccgag gcacgacttg cacggttgaa ggaagaggtt
     3601 gaagcatcgg gccttactcc cgaact
//
LOCUS       DAOQVJ010000113         3622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29150_length_3622_cov_4.264087, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000113 DAOQVJ010000000
VERSION     DAOQVJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29150_length_3622_cov_4.264087"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..1333)
                     /locus_tag="PKL26_03385"
     CDS             complement(371..1333)
                     /locus_tag="PKL26_03385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS82772.1"
                     /translation="MISIVIPNYNGKHFLEGCLSSIAAQTYHDFETILVDNASRDGSA
                     SFVRERFPWVLIVENPLNLGFAGGTNSGIRQAKGQYILTLNNDTRLEPDFLEQVKIAI
                     ESESMVGVVAPKMLLMDGRINSTGMCISRSGAAWNRGMFENDCGQYNAPEPVVGACAG
                     AALYRREVFDDIGLFDEDFFLYHEDVDLSFRAFLAGWNCLYWPSAVVWHENSGTLDYG
                     SDLCIYYVNRNILWFVAKSYPSWLLLSSLPWIIGRNIASVAFYALNGHIRVIVKSKMD
                     ALRGLPAAAGKRSSVKICVSRNKIMKYVRTWWQKESMRQNGAVK"
     gene            complement(1515..2645)
                     /locus_tag="PKL26_03390"
     CDS             complement(1515..2645)
                     /locus_tag="PKL26_03390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS82773.1"
                     /translation="MKILHISKKYPDAIGGDAQVVRSLEIQQEKAGHEILILTANTRE
                     LRKKRNVRIFGIFDTAANLDRITPKRMLSLVCFLFASIRAIAAEKPDVIHTHSAELGF
                     LVSFPASVLGIPVIQTCHGICFNDTGFSPLKRAVEAFLLKYGIFECITTVDGSSIPAF
                     SRLDISHVKYVPNGVDSALFKVKRDAQARPLMQFLCVGRLEHQKGLDILIEAASVLRK
                     ATNDFCITIIGDGSLRDELARQISAGSLDVHVQLLGSVPRDELVRQYACSDAFVLPSR
                     WEGMPLTLLEAWAAGLPVIVTDVGGITQICRDRENALVIQPGKPSQLCRAMRLLMDHP
                     DLGKRLGSEGNHIARERYSWDRIVDTYHFLYREAIQHNSRRK"
     gene            3027..>3622
                     /locus_tag="PKL26_03395"
     CDS             3027..>3622
                     /locus_tag="PKL26_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNS82774.1"
                     /translation="MPIKPGLSPGTLAIDGKHTEGPVRITVIDYDAAHYTERQVASVE
                     DCYPFRDTETVTWINIDGLGNTAVIEKLGKHYQIHPLILEDILNTGQRPKMEDLEGYL
                     YVNLKMLQLAGQEKTIKGEHVSMIIGPNYLLSFQEDIGDVFDPVRERIRKEGRIRRFG
                     PDYLAYALIDDIVDNYFLVIEHLEEEAEDLEEELVQNAT"
BASE COUNT          874 a          978 c          928 g          842 t
ORIGIN      
        1 cttacttctg gacccgcggc tgtcatggca aaccccttgt attctcctta tctcgcaaaa
       61 aacgcattct cgtcaatcgt ccaatcactc tcccaccagg cattccagga gcccactggt
      121 aaatatggaa ccggatttgc ccttgttggc ggaatggtgt gagaaggctt caaagagacg
      181 gtgtcgaact ctattgggat tttctggtta tgccattgtc cctctatgat gtggaagttg
      241 gataccggat gaacgcaaac ttctctcaca cgttcctccc aggatggggc tttcatgtcc
      301 tggggaaaac gatctaaatg aaatcctggg aagaaagaac aacgatacgg attacctctt
      361 cttgcgttca tcatttcacg gccccgtttt gtctcatgct ctccttctgc caccaggtcc
      421 ggacatactt catgatcttg ttcctggaaa cgcatatttt caccgaagac ctctttcctg
      481 ccgcagcggg cagtccgcgc agtgcgtcca tctttgactt tacaatgacg cggatatggc
      541 cattcagcgc gtaaaatgca accgatgcaa tatttctccc gattatccag ggcagggagg
      601 agaggagcag ccacgacgga tacgactttg ctacaaacca gaggatgttc ctgttaacgt
      661 agtagatgca gaggtcggag ccgtaatcaa gcgtcccgct gttctcgtgc cagacaacag
      721 cgctcggcca gtaaagacag ttccatcctg caaggaaggc ccggaaggag aggtcgacgt
      781 cctcgtggta taaaaagaaa tcctcgtcaa agagcccgat atcgtcgaac acttcccggc
      841 ggtagagtgc cgcgcccgca catgcgccga ccactggttc tggagcattg tactggccgc
      901 agtcattctc gaacattccc cggttccagg ccgcaccgct ccgcgaaatg cacatgcccg
      961 tggaattgat gcggccgtcc atgagaagca ttttgggtgc gacgaccccg accatggact
     1021 cggactcgat cgcgatcttc acctgttcga ggaagtccgg ctcaagccgt gtatcgttat
     1081 tgagagtgag aatatactgc cctttcgcct gccgtatccc tgaatttgtc ccgcccgcaa
     1141 acccgagatt cagaggattt tcaacgatta aaacccaggg gaatcgctct cttacaaagg
     1201 atgcgctccc gtcgcgggat gcgttgtcga cgaggatcgt ctcgaaatcg tgatatgtct
     1261 gcgcggcgat ggaggagagg cacccctcga ggaaatgctt cccgttataa ttggggatga
     1321 caatgctgat caccggatgt gtattccctc cctcaccgat tactcctgcg ttcgtatctc
     1381 attatggggg tcacaaggta caatagcttt cgcgacaaga agcgaaataa aacgggtaat
     1441 ctcctcccct ttgtccacac cggaatctcc tggtggtggg cgcaagcatc ggaaacggat
     1501 cattgataac ggcttcattt tcgtcggctg ttgtgctgaa tcgcttcccg ataaagaaaa
     1561 tggtaggtat caacgatacg gtcccatgaa taccgctctc ttgcgatatg gtttccctct
     1621 gatccaagcc tcttcccgag atcgggatgg tccatcagga gccgcatcgc ccggcagagt
     1681 tgcgagggtt ttccgggttg tatcacgagg gcattctccc tgtccctaca gatctgagtg
     1741 atcccaccaa cgtctgtgac aatgacaggg agtcccgctg cccaggcttc aaggagtgtc
     1801 aaaggcatcc cctcccagcg cgatgggagt acgaatgcat cagaacatgc gtactgccgc
     1861 accaactcgt ctcttggcac gcttccgaga agttgcacgt gaacgtcgag cgatcctgca
     1921 gaaatctgcc gtgcaagctc atcccttagc gatccatcgc cgataatagt gatgcaaaaa
     1981 tcatttgttg ctttccttaa aacagatgca gcctcgatga gtatatccag tcccttctga
     2041 tgttcaagcc tccccacgca caggaactgc ataaggggcc ttgcctgtgc gtctctcttg
     2101 accttaaaga gtgcactgtc caccccattg ggaacatact tcacatggga gatgtcgagc
     2161 ctagaaaaag cggggatact gctcccgtcg accgtggtga tacactcaaa tatcccgtat
     2221 ttcagaagga aggcttcgac ggcgcgcttg aggggtgaga aaccagtgtc gttgaagcag
     2281 atgccgtggc atgtctggat tacgggtatg cccaacacac tggcaggaaa tgagacgagg
     2341 aagcccagtt cagccgagtg agtatggatg acatccggtt tttcggctgc aatggcccga
     2401 atggaggcga agagaaagca gaccagcgac agcatcctct ttggggtaat ccggtcaagg
     2461 ttcgctgcag tatcaaagat tccgaagatc cgcacattcc tctttttcct caactctctt
     2521 gtattcgcag taaggataag aatttcatgg cctgctttct cctgttggat ctcgaggctg
     2581 cggacgacct gtgcatcccc cccgatggcg tcaggatact tcttggagat gtgtaagatt
     2641 ttcatatcgg caatttccgg gcgctatatc agatctggtc cgggatgccg ggcggcaatc
     2701 cggtgggtcg tcgtgaagga tcgtcccagg tatctttttt cctcatcact atatcctctc
     2761 tctgattgca attaataaag gaggttactc ttttgggatt gtggggttgg caaacgcggc
     2821 atatcttcat gtctgtggat agaatccgaa cctattcacc ccttcgaaag gagatatctt
     2881 taaccaatac aggagaaata ctcccgatca tcccccagaa aaaactacat gattctcttt
     2941 gagacacaag catacgcatt tatgaaaatg gcgcagccat tttcatagta aacaggaaat
     3001 ctcccatgcc ccgccgccgc cgtgttatgc caatcaagcc gggactctcc ccgggaacgc
     3061 tcgcaataga tggaaagcac accgagggcc cggtaaggat cacagtcatc gactacgatg
     3121 cggcgcatta taccgagcga caggtcgcca gcgtcgagga ttgttacccg ttccgtgaca
     3181 cggagacagt cacctggatc aacatcgatg gcctcgggaa caccgcagtc atcgagaaac
     3241 tcgggaaaca ctaccagatc caccccctca tcctcgagga catcctgaac acgggccagc
     3301 gccccaagat ggaggacctt gaaggctatc tctacgtgaa cctcaagatg ctgcagcttg
     3361 ctggacagga aaaaacgatc aaaggtgagc atgtcagcat gatcatcggg ccaaactacc
     3421 ttctctcgtt ccaggaggac attggcgatg tctttgaccc ggtgagggaa cgtatcagga
     3481 aagaggggag aatccggagg ttcgggccgg attacctggc atatgccctg atagacgata
     3541 tcgtggacaa ttacttcctt gtcatcgagc acctcgagga ggaggcagag gacctcgagg
     3601 aggagctcgt gcagaacgcg ac
//
LOCUS       DAOQVJ010000114         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29352_length_3605_cov_3.923944, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000114 DAOQVJ010000000
VERSION     DAOQVJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29352_length_3605_cov_3.923944"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..1249)
                     /locus_tag="PKL26_03400"
     CDS             complement(341..1249)
                     /locus_tag="PKL26_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNS82775.1"
                     /translation="MLKLSQFRPVTIRDQALFRAHYSRYPQVHSDNLFSNMLCWNHFA
                     NYAYLQVGGAIVLCSTIQGKTRFRPPIGPRDPDLLEEVIHLAIREGDEAPLVLVDTPA
                     REWIQYLYPELPLHEDRDHFEYVYCAEDLARLPGGEYLTLRRQLNRFRKQCRPLLEEI
                     APHNLADVERFLHEWCEWKQCDEHPFLAEEKDAVLYAISHFTELGLSGLLIRIEGKVG
                     AIAVFEEMNRETAVVHFEKGLPDCEGIYKAINAETAAFLEERYRYINRESDMGVPGLR
                     EAKMRYHPHHMVEVSYALHDDLARMV"
     gene            complement(1243..1683)
                     /locus_tag="PKL26_03405"
     CDS             complement(1243..1683)
                     /locus_tag="PKL26_03405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS82776.1"
                     /translation="METNDEELDLFVVREWDSRALVELYRAGGWWKEEWDPADIPLLV
                     RSTFAFVVAVHRQSGRTVGMGRAISDGVSDAYIQDLVVLPEFRGMGVGKAILERLVGI
                     CKERGVCWIALVAEPGTSDFYESIGFEAMEGHIPMKFRGGENRC"
     gene            complement(1745..2584)
                     /locus_tag="PKL26_03410"
     CDS             complement(1745..2584)
                     /locus_tag="PKL26_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS82777.1"
                     /translation="MRLRVLADNSTLIDRYYLAEPGFSALVETEGGKVLFDLGYSDVF
                     QKNAMKMGQSLLDLDYVVLSHGHLDHTWGLFHLVMALTEAQIEKREFKRPVLVAHPEV
                     FLTKQSGKVPESGALISEEKCGEHFGLHLSREPFWLWDDLVFLGEIPRRFKFEPPEPW
                     RKRRRNGGIEPDMMPDDSALAWKGEEGLVVITGCSHAGICNIVAYAREVCGEERVQDI
                     VGGFHLMKAPQSRLDAMASALKEMGVNRMHPCHCTGFRAVHRLAREFQVEETGSGLVC
                     EYR"
     gene            complement(2769..3314)
                     /locus_tag="PKL26_03415"
     CDS             complement(2769..3314)
                     /locus_tag="PKL26_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS82778.1"
                     /translation="MRITVSGLPGSGTTSLARHLASTQNYTLISAGEVFRQMAGEKGL
                     DIAEFGRIAEKNPSIDREIDARQREIAEARDDIIAEGRLSGWCVRNADLRIWLQAPME
                     CRVERIFSRDTIVDIDTALSLTREREASEARRYREYYSIDINDLSLYHMVLNSAKFGV
                     EDLSSLVETAISRIEQAKTVS"
     gene            complement(3314..>3605)
                     /locus_tag="PKL26_03420"
     CDS             complement(3314..>3605)
                     /locus_tag="PKL26_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HNS82779.1"
                     /translation="KRLDAKRDRVMKEQMELSQQQFTPMAYILIITVPIFFWLLFRLG
                     VAPDATVVMPFFGTHTLTDAVLGPVPAWMLWYMICSLSISQVVRKSLNIGGI"
BASE COUNT          770 a         1048 c          973 g          814 t
ORIGIN      
        1 taattctccc accatagttc ccttggaact actatcttag aggttaaatc tccctatttt
       61 aaagctttgc cttattccac acgcgatacg ctctcctttt ccaataatct gggcaaatgt
      121 tgagcctttt tcggaaaaat atgacaaaat cagtcattat tggaaaaagg cacagaattg
      181 cttcaattcg taacgcgcac gtcccatatt tgagggaaaa ataaggccat aaaagccata
      241 ttttttcatg ctatattatc gggaataata taatgtttag tatttttaaa aagaagcctg
      301 attccgccgc gcaggagaga gtgaaggcgt tgcaagaggt tcacaccatg cgggccaggt
      361 catcgtgcaa tgcatatgag acctcgacca tgtggtgagg gtgataccgc atcttcgctt
      421 cacggaggcc cgggaccccc atgtcgctct cacggttgat gtagcgatac ctttcctcaa
      481 ggaatgccgc ggtctctgca ttgatagcct tgtaaatccc ctcgcagtcg gggaggcctt
      541 tttcgaagtg gactactgcg gtctcgcgat tcatctcctc gaagactgct atcgctccca
      601 ccttgccttc gatccggatg aggaggccgg agagcccgag ctccgtgaag tgcgagatag
      661 catacaggac ggcgtccttc tcttctgcga ggaaggggtg ttcatcgcat tgtttccact
      721 cgcaccactc gtggaggaac ctctccacgt cagcgagatt gtgcggtgcg atctcctcca
      781 ggagcggcct gcattgcttc ctgaaacggt tcagctgccg ccggagggtc aggtattctc
      841 ccccgggcag gcgggcgagg tcctctgcac agtatacgta ttcaaagtga tcacgatctt
      901 cgtgaagcgg cagttcagga tacaggtact gtatccactc cctcgcaggc gtgtccacca
      961 gcaccagcgg cgcctcgtcc ccttcccgga ttgcaaggtg tatcacctct tcgagaaggt
     1021 cgggatcgcg gggaccgatg gggggacgga accttgtctt gccctggatc gtgctgcaga
     1081 gcacgatcgc accccccacc tggagatacg cataattagc aaaatggttc cagcagagca
     1141 tgttcgagaa gaggttgtcg ctgtgcacct ggggataacg ggagtagtgg gcccggaaga
     1201 gtgcctgatc cctgatggtg acggggcgga actgcgagag cttcagcacc tgttctcccc
     1261 tccgcggaat ttcatgggga tatgtccttc catggcctca aatcctatgc tctcataaaa
     1321 gtccgaggtc cccggctcgg ccacaagggc aatccagcag actccccttt ctttgcagat
     1381 cccgaccagc ctctctagga tagcttttcc cacccccata cccctgaatt ccggcagcac
     1441 gaccaggtcc tggatgtacg catccgagac accgtccgag attgcccggc ccattcccac
     1501 cgtccggccg ctctgcctgt gaacggcaac gacaaatgca aaggttgacc tcaccaggag
     1561 cgggatgtct gcgggatccc attcctcctt ccaccagccg cccgcgcggt aaagttcaac
     1621 gagcgcccgg gaatcccatt ccctgaccac gaagaggtcc agttcttcat cattcgtctc
     1681 catgtctgcc cacctgtccc aaaaatgaga agtgattgct ctcttcagtg atgaaaattt
     1741 ttacctatct gtactcgcag accagccccg atccagtctc ctcaacctgg aattcacggg
     1801 caagccgatg gaccgctctg aacccggtac aatggcaggg atgcatgcgg ttgacaccca
     1861 tttctttcaa cgcgctggcc atcgcatcaa ggcggctttg cggggccttc atcaggtgga
     1921 acccgccgac gatgtcctgc acgcgctctt caccgcagac ctcgcgtgcg tatgccacga
     1981 tgttgcagat gcccgcgtgg gagcacccgg tgattactac cagcccctcc tcccctttcc
     2041 acgcgagtgc gctgtcgtcc ggcatcatgt cgggttcaat gccgccgttg cgtctcctct
     2101 tcctccaggg ttcaggcggc tcgaacttaa acctccgcgg tatctcccca agaaacacaa
     2161 ggtcatccca gagccagaat ggttcacggg agaggtggag gccgaaatgc tccccgcact
     2221 tctcttcaga tatgagtgcc cctgattccg ggacctttcc ggactgcttg gtcaggaaga
     2281 cctcgggatg ggcaacgagg acaggccttt tgaactctct cttctcgatc tgggcctctg
     2341 tgagtgccat cacgaggtga aacaggcccc aggtatgatc gaggtggccg tgggagagca
     2401 cgacgtagtc gaggtcgagg agggactggc ccatcttcat ggcgtttttt tgaaagacat
     2461 cggagtatcc gagatcaaag agcacttttc ccccctccgt ctccaccagc gcagagaacc
     2521 caggctcggc gaggtagtag cgatcgatga gggtggagtt gtctgccaat acccggagtc
     2581 tcattgggcg cttcctcctc caggaatgat catgccactc cgcttccgca tggatatact
     2641 atctttccgt aatcgacgat aatatggccg catgttcacg caaaagacgt tattcagaat
     2701 gatggctgag gcaatcggcc aggataaaag tttagaggag gagagagtgt gttgtggtat
     2761 gcgtaagttc aggagaccgt ctttgcctgc tcgatcctgc tgatggcggt ctctacgagg
     2821 gaagaaaggt cctccactcc gaactttgcc gagttgagga ccatgtgata gagggagagg
     2881 tcgttgatgt cgatcgagta gtactccctg tagcggcggg cctccgatgc ctcccgctca
     2941 cgggtcagcg aaagggcggt atcgatgtcc actatggtgt cgcgtgagaa tatccgttca
     3001 accctgcact ccatcggcgc ctggagccaa atccttaggt cggcgttgcg cacacaccac
     3061 cccgagagcc ttccctcggc gataatgtcg tcccgcgcct cggcgatctc gcgctggcgt
     3121 gcatcgatct cgcggtcgat cgagggattc ttctcggcga tccttccgaa ctcggcaatg
     3181 tcaagcccct tttcgcccgc catctgcctg aagacctctc cggcagagat aagggtgtaa
     3241 ttctgtgtgg acgcgaggtg gcgggcgagc gaggtggtcc cgctcccggg aagcccgctc
     3301 accgtgattc gcatcaaatg cccccgatgt ttaaggactt cctgacgacc tggctgatcg
     3361 agagggaaca gatcatatac cagagcatcc acgccgggac cgggccgagc accgcatcag
     3421 taagcgtgtg agtcccgaag aatggcatga ccacggttgc atcgggtgca accccaagcc
     3481 tgaagaggag ccagaagaag atcggcacgg tgataatcag gatgtatgcc atgggagtga
     3541 actgctgctg cgaaagctcc atctgctctt tcatcacgcg atctctcttt gcatccagtc
     3601 tcttg
//
LOCUS       DAOQVJ010000115         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29491_length_3592_cov_3.858355, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000115 DAOQVJ010000000
VERSION     DAOQVJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29491_length_3592_cov_3.858355"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PKL26_03425"
     CDS             complement(<1..161)
                     /locus_tag="PKL26_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HNS82780.1"
                     /translation="MDCTEGRIGRVFVLRIDHGEDILETLLQCIREKEIFCGTVQVLG
                     ALGKGQMVTG"
     gene            complement(192..2009)
                     /locus_tag="PKL26_03430"
     CDS             complement(192..2009)
                     /locus_tag="PKL26_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNS82781.1"
                     /translation="MGETRELLLLVVLGFVLGAAAVLVSLAIPVLLEGDLVVDRYQAT
                     LQEDGTFQEVYTYEVKSSGQYRMLYRYWQDRLSSQPLDSPYIWFVEMDYPAGNVGYMK
                     DWAGKVTLFGDDNPLYIRFVEDRAERNEVGIYNPGYFPAGKYTVSYTYVLHPPIEYDK
                     IDAHLNLRLVDEHVPFRTVEIRIPERYAGEVFPHPPGLAVTREGGGVLIKGSVAADET
                     LGFELLFPAAEAAGIPGFSSYVEDVRGKTRAANSIIQQIPLLGAWALYGSGVVMIFLT
                     PLLLLLIHRRFGREKEFTVPEFLSFTPNPAMKPWAVNLLFKGDAIEFDQDGYYATLLD
                     LHRRRVITIREKTDGKGITITVNEWQSEEPYEQRVLDFLRNVEEEGIIDSDSLQKMAV
                     LARSDREVEKRMLGYQQQLSGVTKKSDPRLISQYIVDGRDHLFPLLFIGIAFCAVSVM
                     VLIVVPVLAAILGPAVVLWAGVIVQSGVALAFPSTLFGHWKDDRYKEKLEWDSFARFL
                     SDLALIKQYSPQDISMWGEWLVYGTALGVGEKVEKAMETLHISLPEAGVPLAMGLRTA
                     FIPVLAFSAPSHGGSGGGFGGGGSFGGGGGFGGGGVGGR"
     gene            complement(2023..2595)
                     /locus_tag="PKL26_03435"
     CDS             complement(2023..2595)
                     /locus_tag="PKL26_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNS82782.1"
                     /translation="MALGDLIPWIAGGIILLVIIGLVLYFVGIYNRFFSLKNSSEATL
                     GQIRVAMKKRLDMIDQLLGAVKSYAAFERETFEKVTAMRTSVGSAGPGDLNQIERESR
                     SLLGRLFAVAENYPDLKTNATVMNLMDAVRGVEDEIARQRYTYNNISQQYNTLRDVIP
                     SNIVARILGLSKLEYLEFEEAIQTPPKIAF"
     gene            complement(2702..>3592)
                     /locus_tag="PKL26_03440"
     CDS             complement(2702..>3592)
                     /locus_tag="PKL26_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNS82783.1"
                     /translation="SDITFYNQKRDETTVTYIEPSRYPEKLSSLFFSISMAGMALLVV
                     DEINAAFGECVLMLDMAKVRKGVLVLRNYLSPEQVAPLIKGTVVEHFQVHSDDHGQLR
                     ELFLEEAANASQPAEGSKGGSVPVDHHFPVKGVGIVVLGGVAEGTIRAHDQIKVLPTG
                     KVAHLRSIQKHDDDVSEASAGDRVGLALKGVEEVDLDRGYVLSTNPSLVCSSILNGRA
                     ELVRYWPAPLKEQMIVYLGHWMQFIPARIAFVNNSGDWKRPEITLRSEKELVYPPGAP
                     ILIHYLEGGKLRVVGRIVPA"
BASE COUNT          740 a         1128 c          983 g          741 t
ORIGIN      
        1 cccgtcacca tctgcccctt cccgagtgcc ccgagcacct ggacagtccc acagaaaatc
       61 tccttctccc ggatgcattg caaaagtgtc tcaaggatgt cttctccgtg atcgatgcgg
      121 aggacaaaga cccggccaat gcgtccttca gtgcagtcca tgaagacctc cccatacaga
      181 cgccccatac ttcatcggcc acccactccc ccgcctccga atcctccgcc tcccccaaaa
      241 gatcctccgc ctccgaaccc tcccccgctt cctccgtgcg atggcgctga gaatgcgagg
      301 acggggataa acgcggtccg gagccccatc gccagcggaa ctccagcctc tggcaggctg
      361 atgtggagag tctccattgc cttctccacc ttctcaccca ccccgagcgc cgtcccatag
      421 acaagccatt caccccacat cgagatgtcc tgggggctgt actgcttgat cagggccagg
      481 tctgagagga aacgggcaaa cgaatcccac tccagcttct ccttgtaccg gtcgtctttc
      541 cagtgaccaa aaagcgtgga tgggaatgca agggctaccc cggactggac gatcaccccg
      601 gcccaaagga caactgcggg cccaagaatg gctgcgagaa cgggaaccac gatgagcacc
      661 atgactgaga cagcgcagaa cgcgatgcca atgaagagga gagggaagag gtggtccctc
      721 ccgtcgacga tatactgcga gatgaggcgg ggatctgact ttttcgtaac ccccgagagc
      781 tgctgctggt acccaagcat cctcttctcc acctccctgt cactccttgc caggaccgcc
      841 atcttctgga gggagtccga gtcgatgatc ccctcctcct cgacgttgcg gaggaagtcc
      901 agcacccgct gctcgtatgg ctcctccgac tgccactcgt tgacggtgat ggtgatgcct
      961 ttcccgtcag tcttctctct gatggtgatc acgcgccttc tatgaaggtc gaggagggtg
     1021 gcataatacc cgtcctggtc gaactcgatc gcatccccct tgaaaaggag gttcactgcc
     1081 cagggcttca tcgccgggtt gggggtaaaa ctcaaaaact ctggaactgt gaactctttc
     1141 tcccggccga acctcctgtg aatgaggagg agaaggagcg gggtgaggaa tatcatcacg
     1201 accccggaac cgtagagtgc ccatgcaccc aggaggggga tttgttggat gatggagttt
     1261 gcggcccggg tctttcccct cacatcctca acgtaactgg aaaaacccgg gatgcccgct
     1321 gcttcagcag ccggaaagag cagctcaaaa ccaagggtct cgtctgctgc aacactcccc
     1381 ttgatcaaca cccctccgcc ctcacgcgtg actgcaaggc ctggcggatg ggggaagact
     1441 tcgccggcat accgttcggg gattcggatc tcgacagtcc ggaacgggac atgctcgtcc
     1501 acaaggcgaa ggttcaggtg agcgtcaatt ttgtcatatt cgataggagg atggagaaca
     1561 tacgtatacg agacagtata ctttccggct gggaaatatc cagggttgta gatcccgacc
     1621 tcgtttctct cggcacggtc ctcgacaaac cggatgtaaa gtggattatc atcaccgaag
     1681 agtgtgacct tcccggccca gtctttcatg tatcccacgt tgcctgccgg atagtccatc
     1741 tccacgaacc agatgtacgg cgagtcgagc ggctgggacg agagccggtc ctgccagtat
     1801 cggtacagca tccggtactg cccgctgctc ttcacctcgt acgtatagac ctcctggaac
     1861 gtgccgtcct cctggagcgt agcctggtac cggtcaacga ccaggtctcc ctccaggagt
     1921 acgggtatgg ccagtgatac gagtactgca gctgcaccca gcacgaagcc gagcacgacc
     1981 aggagcagga gttctctcgt ctctcccatg acccggctcc gttcagaacg caatcttggg
     2041 cggtgtctgt atggcctctt caaactccag gtactcgagc ttggacagcc caaggatcct
     2101 ggcaacgata ttcgagggta tcacatcgcg gagggtgttg tactgctggg agatgttgtt
     2161 gtaggtgtac cgctggcggg ctatctcgtc ctcgacgccc cggaccgcgt ccatcaggtt
     2221 catcactgtc gcattcgtct tcaggtccgg gtaattctcc gcgacggcaa agagccgccc
     2281 gaggagggag cgcgattccc gttcgatctg gttgaggtcg ccaggcccgg cgctccctac
     2341 gctcgtccgc atggcagtga ccttctcgaa ggtctcacgc tcgaaagcgg cgtagctctt
     2401 gaccgcaccc aggagctggt cgatcatgtc gagtcgtttc ttcatggcca cccgtatctg
     2461 gccgagtgtc gcttcagacg agttcttcag gctgaagaac cggttgtata tcccgacaaa
     2521 atagagcacc agtccaataa ttacgaggag gatgatgccc cctgcgatcc aaggtatgag
     2581 atccccaagt gccataactt cacctatctc ccgcatatct atttaagtcc acgtcccgga
     2641 acgccgcaag agtgctcctc cgctctgcaa attccgggtg gttacgccaa ctacaccgcg
     2701 ctcatgccgg cacgatcctt cctaccaccc ggagctttcc cccctccagg taatggatga
     2761 ggatcggtgc tcccggaggg tataccagtt ccttttccga ccgcagcgtg atctcgggtc
     2821 tcttccaatc tccggaattg ttcacgaatg cgatcctggc agggatgaac tgcatccagt
     2881 gcccaaggta tacgatcatc tgctctttca ggggcgcagg ccagtaccgg accagctcgg
     2941 ccctcccgtt caggatactt gagcacacga gggagggatt ggtcgagagg acgtatccgc
     3001 ggtcaaggtc gacttcctcc acccccttca gcgcaagacc cacccggtcc cctgccgacg
     3061 cctcggagac atcgtcgtcg tgcttctgga tggagcggag gtgggcaacc ttcccggtgg
     3121 ggagcacttt gatctggtca tgcgcccgga tggtcccctc cgcgacgccg ccgaggacca
     3181 cgatccctac ccccttaacc gggaagtgat gatcgacagg gacggatcct cccttcgacc
     3241 cctcggccgg ttgcgatgcg ttcgcggcct cttcgaggaa caactccctc agctgcccgt
     3301 ggtcatcgga atggacctgg aaatgctcga ccactgtgcc cttgatgagc ggggctacct
     3361 gttcggggga aaggtaattc cgaagtacaa ggactccctt tctcaccttc gccatgtcca
     3421 gcatcaggac gcactccccg aacgccgcat tgatctcgtc gacgacgagg agagccattc
     3481 ccgccatcga gatggagaag aagagagagg agagcttttc cgggtaacgg ctcggctcga
     3541 tataggtgac ggtggtctcg tccctcttct ggttgtagaa cgtgatatcg ga
//
LOCUS       DAOQVJ010000116         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29535_length_3589_cov_3.853141, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000116 DAOQVJ010000000
VERSION     DAOQVJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29535_length_3589_cov_3.853141"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..876
                     /locus_tag="PKL26_03445"
     CDS             190..876
                     /locus_tag="PKL26_03445"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HNS82784.1"
                     /translation="MQLLVSPRDIEDARRSLAADIVDVKKPSEGSLGANFPWVVRSIK
                     ALSPKPVSAAIGDFDFRPGGASMAAYGAACAGADYIKVGLMFDGAERARELIQAVVRA
                     VKEEFPEKKVVIAGYSDYQRLGSISPFVISPLAAEAGADVAMVDTGIKDGRSTFEFMS
                     EEALVEFCSVNRESGMATALAGSLRFEDIPALKRIGPDIIGVRGMVCGGDRNDGIREE
                     LVDQLVRMVH"
     gene            879..2345
                     /gene="guaB"
                     /locus_tag="PKL26_03450"
     CDS             879..2345
                     /gene="guaB"
                     /locus_tag="PKL26_03450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNS82785.1"
                     /translation="MFRDKLEVPLALTFDDVLLIPAPSLVEPNEADIHTRFSRNISLN
                     MPLVGAAMDTVTESAMAMALAREGGIGVIHRNMSPEQEIEEVRRVKRAEELIERNVVS
                     VSPETSVSDVERLMVEHGIGGVPVLEHEKIVGIVSRRDVRAIASRRGSESVKAIMTRQ
                     PITAGEDISMEQALETMYTNKVERLPVVDKEGHLIGIITMQDILEKRQYPNATRDARK
                     KLRVAAAVGPFDIERAHRLDECGVDALVVDCAHGHNLNVVKAIRDIKGSTNADIVAGN
                     IATAAAAEDLIDTVDGLKVGIGPGSICTTRIVAGVGVPQITAIASVADVTRDTGIPVI
                     ADGGIRFSGDVAKAIAAGADCVMIGSLFAGTDESPGRVITMKGRRYKQYRGMGSLGVM
                     SGGVSSDRYFQKKEIGKTKFVPEGVEGVTPYVGTVSDVVYQLVGGLKSAMGYTGSRTI
                     QEMHEKTRFVRITAAGHTESHPHNIMITDEAPNYRISD"
     gene            2450..3067
                     /locus_tag="PKL26_03455"
     CDS             2450..3067
                     /locus_tag="PKL26_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS82786.1"
                     /translation="MLDQVEVARLLDILGNRNRRRIIELLRQKPCFVTEISDRLMLSP
                     KAVIEHLQLMEREEILAFRLDERRRKYYFLANDIDVMVRLLRQDQINLVIKDNSHRLR
                     SALARLQKMVETREELAESLKNLERDIDQQIKEIISFGHDVLENETQIDLLISLAHCN
                     LTIQELSELVEAPRDAVSDSLERLRKKHIIEREGETFRLCDAHAQ"
     gene            3057..3494
                     /locus_tag="PKL26_03460"
     CDS             3057..3494
                     /locus_tag="PKL26_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82787.1"
                     /translation="MPSNENPYDEFIRNIARMVEDLLKNMPEENTTRIIGCTIITGNS
                     PNKPHVIFNGDRQDDEITFEVIEGPDRIFVTANLPAFLHSAPYADITPESLHIVVNEK
                     RTKVELPCRVDVIHSYYHVRHGVIDIILKKKAAAPANAGIGQG"
BASE COUNT          831 a         1007 c         1083 g          668 t
ORIGIN      
        1 gtttcggggc gagttgctgc acgtatcccg caggatctgc tgttgatgag agcccccagg
       61 tgagcgggag gtaggaacca cggttccccg atgccccgtg cactaccaag ccggccttca
      121 aatccatggc caaaatcaat tccatgctat tagcgcaatc actattagcc agcaggatca
      181 ttaaatccta tgcaattact tgtcagcccg agggacattg aggatgccag acgttctctt
      241 gctgctgata tcgtggacgt gaagaagccg tccgagggat ctctgggtgc aaactttcca
      301 tgggtggtac ggagcatcaa ggccctctcc ccgaagcctg tcagcgccgc aatcggtgat
      361 ttcgatttcc ggccaggggg cgcttccatg gccgcatacg gagctgcatg cgcaggggct
      421 gactatatca aggtagggct gatgttcgac ggggccgaac gtgcgaggga gctgatccag
      481 gccgtcgtgc gggccgtcaa ggaggagttc cctgagaaga aggtggtgat cgcgggctac
      541 tcggattacc agcgcctggg gagcatctcc ccctttgtga tatcccccct tgctgcggag
      601 gccggggccg atgttgcaat ggtcgacacc gggataaagg acggacgcag cacgtttgaa
      661 ttcatgagcg aggaggctct cgtggagttc tgctccgtca accgggagag cggcatggca
      721 accgcacttg cagggtccct ccgcttcgag gatattcccg ccctgaagcg aatcggcccc
      781 gacattatcg gggtcagggg aatggtgtgc ggtggagacc ggaatgacgg aatacgcgaa
      841 gaactggtcg accagctggt caggatggtg cactgaccat gttcagggat aagctcgaag
      901 tcccgctggc cctgaccttt gatgacgtcc tccttatccc tgcaccgtcg ctcgtcgagc
      961 caaacgaagc cgacattcat acccgtttct cacgtaacat ctccctgaac atgccgctcg
     1021 tgggagcggc aatggacacc gtgacggaat cggcaatggc catggcgctg gcccgcgagg
     1081 ggggcatcgg ggtcattcac cggaacatgt cacccgagca ggagatcgag gaggtacgcc
     1141 gggtcaagcg cgctgaggag ctgatcgagc ggaacgtcgt ctcagtcagc cctgaaacga
     1201 gcgtctcgga cgtcgagcgc ctgatggtgg agcacgggat cgggggagtt ccggtcctcg
     1261 aacacgagaa gatcgtgggg atagtaagcc gaagagacgt ccgtgcaatc gcttcacgcc
     1321 gcggctccga gagcgtgaag gcaatcatga cgaggcagcc gatcactgcc ggtgaagaca
     1381 tctccatgga gcaggcgctc gagaccatgt ataccaacaa ggtcgaacgg ctcccggtgg
     1441 tggacaagga gggccacctg attgggatca tcaccatgca ggacatactc gagaaacgcc
     1501 agtacccaaa tgccacacgc gacgcaagga agaaactcag ggttgccgcc gcggtcggac
     1561 cgtttgacat cgagcgggcg caccgcctcg acgaatgcgg cgtagatgca ctggtggtgg
     1621 actgcgccca cggccataac ctcaacgtgg taaaggcaat cagggacatc aaggggagca
     1681 caaatgcgga tatcgttgcc ggcaacatcg ccaccgccgc cgcggccgaa gacctgatcg
     1741 ataccgtcga cgggctgaaa gtcggtatcg ggccggggtc gatctgcacg acccgcatcg
     1801 tggccggggt cggcgtcccc cagatcaccg ccatcgcgag cgttgcagac gtgacaaggg
     1861 acaccggaat cccggtcatc gccgatgggg ggatacggtt cagcggggac gtggccaagg
     1921 ccatcgcggc aggagccgac tgcgtgatga tcggaagcct ctttgcaggg accgacgagt
     1981 cgccgggaag ggtaatcacg atgaaagggc gccgttacaa gcagtatagg ggaatggggt
     2041 ctcttggcgt gatgagcggc ggagtctcaa gcgaccggta tttccagaag aaggagatcg
     2101 ggaagacaaa gtttgtcccg gagggagtgg agggtgtgac cccctacgtt ggcactgtct
     2161 ccgatgtcgt ataccagctt gttggcgggc tcaagtctgc gatggggtac acgggatcaa
     2221 ggacaatcca ggagatgcac gagaagacaa ggttcgtgcg gatcaccgct gccgggcata
     2281 ccgagagcca cccccacaac atcatgatca ctgacgaggc tccgaattac cggatctcgg
     2341 attgaagacc caattctttt tcgatcggaa aaatctctct tttataccgg cttcgctggt
     2401 gaaatcttcc caaggtataa atacttccat aactaacatg aagtaatcaa tgctggacca
     2461 ggtagaggtt gcccgactcc ttgacatcct tggaaaccgg aacaggcgcc gtatcatcga
     2521 gcttttgaga cagaagccct gtttcgtcac cgagatctcc gaccgcctga tgctctcccc
     2581 gaaagccgtg atagagcacc tccagctgat ggagcgcgag gagatcctcg cgtttcgcct
     2641 cgatgagcgc aggaggaaat actacttcct cgcaaacgat atcgacgtga tggtcaggct
     2701 tctcaggcag gaccagatca acctcgtcat caaggacaat tcccaccgcc ttcgttccgc
     2761 gcttgcccgg ctccagaaga tggtggagac cagggaggaa ctggcagaga gcctgaagaa
     2821 ccttgagagg gacatcgatc agcagatcaa ggagatcatc tcgtttggac acgatgtcct
     2881 ggaaaacgag acccagatag acctcctcat cagcctggcc cactgcaatc tcaccatcca
     2941 ggaactctcg gaactcgtgg aggccccgag ggatgccgta tccgatagcc ttgaaaggct
     3001 ccggaagaaa cacattattg agcgggaagg ggagaccttc cgcctctgtg atgcacatgc
     3061 ccagtaacga gaacccctac gacgagttca tcaggaacat cgcgaggatg gtggaagacc
     3121 tcctgaagaa catgccggag gagaacacca cccgcatcat cggctgcacg atcatcaccg
     3181 ggaacagtcc gaacaagccc cacgtcatct tcaacggcga ccggcaggac gacgagatca
     3241 catttgaagt gatcgagggg ccggacagga tctttgtcac cgctaacctg cccgccttcc
     3301 tgcactcggc cccctacgca gacatcacgc ctgaatccct gcatatcgta gtgaacgaga
     3361 agcggacgaa ggtggaactc ccctgcaggg ttgacgtgat ccacagttac taccatgtcc
     3421 gccacggggt cattgacatt atcctgaaga aaaaagccgc cgcccccgcg aatgcgggga
     3481 tcgggcaggg gtagtgccgc tgccctatta ttccgtccct tttttcgaat gaaaatcact
     3541 tcgtgatttt catcgtttat cttcgacggc agggcggtca taagcggtt
//
LOCUS       DAOQVJ010000117         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29547_length_3588_cov_3.701953, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000117 DAOQVJ010000000
VERSION     DAOQVJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29547_length_3588_cov_3.701953"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PKL26_03465"
     CDS             <1..1035
                     /locus_tag="PKL26_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNS82788.1"
                     /translation="PAAENHVYMRRFAAEQNIRNLDIREGICHQVVMERGRAAPGDIV
                     VGADSHTCMYGAAGAFSTGIGSTDMGFVLKFGALYFRVPTTIRLAIEGPFGSRVGPKD
                     LILAIAGEIKADGANYQALEFTGSTLETMDMAGRMTCCNMAVEMGAKAGIVPPDGVTW
                     EYLRARRPVNPFSLASDSDARFTSVHRFDADRLSPKVAVPHNVDQVVDVEKVAGTPID
                     QVFIGSCTNGRFEDLREAAEVLGNRTFHDSVRVLVIPASRQEYLKALRAGLIEQFVSA
                     GALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFRGRQGSTESMVYLCSPATAAASAI
                     RGEITDPREV"
     gene            1038..1535
                     /locus_tag="PKL26_03470"
     CDS             1038..1535
                     /locus_tag="PKL26_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNS82789.1"
                     /translation="MRTWKFGDNIDTDAIIPGRYLVIYDPRTLASHAFEGTRNEFAAG
                     AREGDVIVAGRNFGCGSSREHAPLALSGAGVRFIVARSFARIFYRNAINVGLLPLVCM
                     DADKVEDGSELTVNLPGGYVESGGKKYRIEPVPAFLQEIVDAGGLVVYAKNLDEVKTC
                     TGSQS"
     gene            1514..2629
                     /locus_tag="PKL26_03475"
     CDS             1514..2629
                     /locus_tag="PKL26_03475"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNS82790.1"
                     /translation="MHRIAVIGGDGIGPEIIREGTKVLEAAGDRFGFSLAFEEFDIGA
                     DRYLSTGELLTDEDIEALSRFRAIYFGAIGDDRVKPGILEKGILLALRFHFDQYINLR
                     PIRLLEGVATPLAGKNPSDIDFVVVRENTEDFYVGIGGRFRGTQKTVLGIQRELYQAR
                     FGLDLESDAEEIAYQIGVVTREGSRRVIQFACELAERRRKKLTSVDKANVLSDVYGLW
                     REVFSEVTAGYPDLATEFNFVDAVTMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGG
                     MGLAPGGNINPAGTSMFEPIHGSAPKYRGQGVANPMATIWAGSMLLDYLGEHEAAEGV
                     LKALGQSILDGVVTRDMGGSARTSDVGDYVAAMIKRG"
     gene            complement(2755..3201)
                     /locus_tag="PKL26_03480"
     CDS             complement(2755..3201)
                     /locus_tag="PKL26_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82791.1"
                     /translation="MQRAGYLSRDGKRVLDEDGVPREILELNIYGARLCILIDDLIAA
                     IQEGTAAPVERVKQNWMEYLGGQAGRVQVSRSGRALNIYLMNGDRFTLSLDSLREVLG
                     YRERAARIVELPPLPPQEAARDHLITDYCRPLSHFNTHETADSMTA"
     gene            complement(3315..>3588)
                     /locus_tag="PKL26_03485"
     CDS             complement(3315..>3588)
                     /locus_tag="PKL26_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNS82792.1"
                     /translation="SRNTPSHGRFPVEMARDYATVSELLERERPDLVLGSSFEQAVCP
                     TAAFVGVTPPLRGAVRLHARPLAGIEGALALMESVLNACMDHKKPA"
BASE COUNT          768 a          994 c         1094 g          732 t
ORIGIN      
        1 cccgcagccg agaaccatgt atacatgcgg cgatttgcag ccgaacagaa catccgcaac
       61 cttgacatca gggaggggat ctgtcaccag gtggtgatgg aaagaggaag ggccgcaccg
      121 ggagatattg tcgtcggggc cgattcccac acttgcatgt atggggcggc aggggcgttc
      181 tcgaccggta tcggctccac agacatgggt tttgtcctca agttcggggc cctgtatttc
      241 cgggtcccga cgaccatccg gctggctata gagggacctt tcggctccag ggtcgggcca
      301 aaagacctga tcctggcgat tgccggggag ataaaggcag acggggccaa ctaccaggcg
      361 ctcgagttta cggggagtac gctggagacc atggacatgg cggggaggat gacgtgctgc
      421 aacatggctg tagagatggg tgcgaaagca ggcatcgtcc ctcccgacgg cgttacctgg
      481 gaatacctga gagccaggcg gccagtgaac ccgttctctc tcgcaagcga cagcgacgcc
      541 cggtttacct ccgtccacag gtttgatgca gacagactct caccgaaagt tgcggtaccc
      601 cacaatgtag accaggtggt tgacgttgag aaggtcgccg ggaccccgat tgaccaggtc
      661 ttcatcggct cgtgcacaaa cggcaggttc gaggatctgc gcgaggccgc agaagtgctc
      721 gggaaccgaa ccttccacga ctcggtccgt gtacttgtga tccccgcgtc ccggcaggag
      781 tacctgaagg ccctgcgggc cgggttgatc gagcagttcg tgtccgcggg ggcgctcgta
      841 gaggccccct gctgcggccc atgcatgggc ggtgcattcg gcctgcttgc acccggggag
      901 gtctctctct cgacatcgaa ccgtaacttc cggggccgcc agggcagcac cgagtcgatg
      961 gtctacctct gttcacctgc caccgcagct gcgagcgcga tccggggaga gatcaccgac
     1021 ccgagggagg tgtgaagatg aggacatgga agttcgggga caacatcgac accgatgcca
     1081 tcattcccgg caggtacctg gtcatctacg atccacgtac gctggcgtcc catgcattcg
     1141 aaggaacacg aaacgagttt gcggcagggg cccgtgaagg ggatgtcatc gttgccggaa
     1201 ggaatttcgg atgcggctcc tcacgcgagc atgctcccct tgccctctcg ggcgcagggg
     1261 tccggttcat cgtggcaagg tcattcgcac ggatatttta tcggaatgcc atcaacgtgg
     1321 gtctcctccc cctcgtttgc atggatgctg acaaggttga ggacggatct gaactcactg
     1381 tcaatttacc aggcggctat gttgaatccg gtggaaaaaa gtaccggatc gagcccgtgc
     1441 cagccttcct gcaggagata gtggacgcgg gcggcctggt ggtatatgca aaaaatcttg
     1501 atgaggtgaa gacatgcaca ggatcgcagt cataggcggg gatggaatcg gccccgagat
     1561 tatcagggaa gggaccaaag tcctcgaggc ggcaggggat cgtttcggtt tctcccttgc
     1621 cttcgaggag ttcgatatcg gggcagatcg ctatctctcg accggggaac tgctcactga
     1681 tgaggacata gaggcactct cgaggtttcg ggcaatctac tttggtgcga tcggagacga
     1741 cagggtgaag cccgggatac tggagaaggg gatcctcctt gcactcaggt ttcacttcga
     1801 ccagtacatc aacctccggc ccatacgcct gctggaagga gtcgcaacac ctcttgcggg
     1861 aaaaaaccca tccgacatag atttcgtcgt ggtgcgggag aacaccgagg acttttatgt
     1921 cgggattggc ggacgcttcc gggggacgca gaagacagtg ctcgggatcc agagggaact
     1981 ctaccaggca aggttcggcc ttgacctgga gagcgatgca gaggagatcg cctaccagat
     2041 cggagttgtc acccgtgaag gctcacgccg ggtaatccag ttcgcctgcg aactcgccga
     2101 aaggagaaga aaaaaactca cttcggttga caaggcaaac gtcctctccg atgtctacgg
     2161 gctctggaga gaagtattct cggaagtgac tgccggatac cccgatctcg ccacggagtt
     2221 caacttcgta gacgctgtca caatgtggtt cgtgaagaac cccgagtggt tcgacgtggt
     2281 cgtcaccccc aacatgttcg gggatatcat caccgatctc ggtgcgatga ttcagggcgg
     2341 gatggggctt gccccgggag gaaacatcaa tcccgcgggc acctccatgt tcgagccgat
     2401 acacggatct gcaccaaaat acaggggaca gggtgtggcc aatcccatgg ccaccatctg
     2461 ggccggatcc atgctcctcg attaccttgg agagcatgaa gcggcagaag gcgtgctgaa
     2521 agcgctcggc cagtcaatcc tcgatggggt agtgacccgg gacatgggtg ggtctgcccg
     2581 tacctctgat gtgggagatt acgtggctgc catgatcaag cgggggtgag accaggtgcg
     2641 ggacatctcc cttttccctc cccctcttag aagatacacg caataccgga tggcgtaaga
     2701 tctcaggaag aaaacgggtc tcccccattt tcccatattg ccacatctct tcgttcaggc
     2761 cgtcatactg tccgcggtct cgtgcgtgtt gaaatgggat agtggcctgc agtaatcggt
     2821 aatgaggtgg tcccgggcag cctcctgcgg aggtaacggc gggagttcta caatccgggc
     2881 tgcacgttcc cggtagccaa gcacctcacg aagagagtca agagaaaggg tgaaccggtc
     2941 gccgttcatc aggtagatgt tgagcgctct cccagaacgt gatacctgca ctctccccgc
     3001 ctggcctccc agatattcca tccagttctg cttgaccctc tctacaggtg cagcagtacc
     3061 ttcctggatt gcagcaatga ggtcatcgat gaggatgcag agccttgcac cgtagatgtt
     3121 gagctccagg atctcgcggg ggacgccatc ctcatcaagg actcgctttc cgtcccggct
     3181 cagatatcct gcccgttgca tgtcatccac ctctcctggt tccattgtct ggagcatttg
     3241 gtataaatag gctcgatatg cgcggggtga aagtgaataa taaaggggag tggaagtcat
     3301 ccttccaggc atcatcatgc aggttttttg tgatccatgc atgcattcag gaccgattcc
     3361 atgagtgcaa gtgccccttc gatccctgcc aggggcctgg catgaagcct caccgcaccc
     3421 cttaatggtg gagtcacccc gacaaatgcc gccgtgggac atacagcctg ttcgaatgaa
     3481 gacccaagga ccaggtcagg gcgctcacgt tcgagaagtt cgctgactgt cgcgtagtcc
     3541 cttgccatct ctacagggaa ccgcccgtgg gaaggggtat ttctggaa
//
LOCUS       DAOQVJ010000118         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29561_length_3586_cov_4.473237, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000118 DAOQVJ010000000
VERSION     DAOQVJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29561_length_3586_cov_4.473237"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PKL26_03490"
     CDS             <1..543
                     /locus_tag="PKL26_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNS82793.1"
                     /translation="ILSRIREFVSSSLSNRHPWVSLFAVNTLMVPFVFMVQMLLLVLF
                     FNLPMPLSLVLLIVSAAFVEEIAKSLGISTFLIENPARFSWRWVVIGSAVTAAAFLFA
                     EKLLLLVTLAQVTESIFGSILFLSSGALLLPFLLHFIGVLCVGGLVRFKGARGYLPGI
                     LLATLVHCLYNGYFILGWLL"
     gene            540..1619
                     /locus_tag="PKL26_03495"
     CDS             540..1619
                     /locus_tag="PKL26_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82794.1"
                     /translation="MNTRNAALVMKKDLRGLSRERTIVLAIMLQLFVALFSSFLMVGL
                     TSMYDPSALARFSGVKYQVGYVGESPELENLLRESNQFQVFPMDLSTAVAALGERKLS
                     AVIHVPDTAIDAEEPVKITLYTLQNDIQSTIVNVKLKEVFLDYEEELRQVRSGRLDLI
                     PIPLEFPASRAGPGFYEFIYGLLIPLLLFMPAIISAALIIDLITEEYQHHTLETLLST
                     PMTMAEVVWGKVAACVVLVPLQAGTWILLLALNRIHIQNILQILAHVISFSLVLVLIG
                     ALTALYYRERTAAQFIFSTTVVVVMLVVLAFPGNPLNLIALLATGAAGMGQWSVLGVT
                     LLFCCGLGLLTHVYAGRVSTRAPGE"
     gene            1775..2413
                     /locus_tag="PKL26_03500"
     CDS             1775..2413
                     /locus_tag="PKL26_03500"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNS82795.1"
                     /translation="MYCPKCGRETESGGRYCQWCGADTRVAPPRAVLRKKVGDISTEK
                     YAGLGRRIGSGLIDILFLILFDMIAVGMISIISWLLQRPCPISESIVMLYQYYRHVPR
                     TDAYGNVVNAAIPSQIVFSAGILFLLIPWIYFAWLESSRNQATLGKMVARIAVTDMQG
                     SRITFARGTLRFFAMSLSVLTLCVGFIIPAFTRYKQGLHDIIAGTLMFRQDP"
     gene            2583..>3586
                     /locus_tag="PKL26_03505"
     CDS             2583..>3586
                     /locus_tag="PKL26_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82796.1"
                     /translation="MAASSPLSRLSLRSSLILCITAIILIVIMFLMAAAYAQAYSWTV
                     QQDHEQALFTEMYLEESILLADRGLSLYDSTLDSRMEEAFLGYLSAYHASEGDISRID
                     LPAIRDALDSGFRGQLDLYIINESGVIVASTVPGVMYLDFKNYPEFFRSITRIREGDE
                     FVADRVVRSVVNTTEGTVSGQLRKFAFYPTPDHRYLLEMGLVMEGFENQRSELSYVEV
                     TRRIQEMNPSIELIRIFDVNGNLLLERGVNPHTGANQTFIGEIFSTGKDAEILEQDRN
                     RRLHYIYVDLRESSTASDMSLVAEITYTQEPLRKALSQILLYYTLIGFIAIVMGIVMA
                     "
BASE COUNT          713 a         1080 c          931 g          862 t
ORIGIN      
        1 atcctatccc ggataaggga gtttgtgtca agctcgctct ccaaccgtca cccatgggtc
       61 tccctctttg cggtaaacac cctgatggtg ccgttcgtct tcatggtcca gatgctcctt
      121 ctcgtcctct tcttcaacct ccccatgccg ctctcgcttg tcctcctcat cgtatccgcg
      181 gcatttgtgg aggagatcgc aaagtctctt ggcatcagca ctttcctcat cgagaatcct
      241 gcccgcttct cgtggagatg ggtggttatc ggctcggccg tcaccgcggc agccttcctc
      301 tttgccgaga aactcctcct cctcgtcacc ctcgcacagg tgaccgagag catattcggg
      361 agtatcctct ttctctcatc gggagcgctc ctcctcccgt tccttctcca ctttatcgga
      421 gtcctctgcg tgggaggcct cgtccggttt aaaggtgcgc gcggctatct ccccggtatc
      481 ctgctcgcaa ctcttgtcca ctgtctctac aacggctatt tcatcctggg gtggctgctg
      541 tgaacacgag gaatgcagcc ctggtgatga agaaggatct ccggggtctt tcgcgggagc
      601 ggacgattgt ccttgccatc atgctccagc tctttgtcgc actcttctcc tcgttcctga
      661 tggtagggct cacctcgatg tatgacccct ctgctctcgc ccgcttctcg ggagtgaagt
      721 accaggtcgg gtacgtgggg gagtcccctg aactcgaaaa ccttctaagg gagagcaacc
      781 agttccaggt cttcccgatg gatctctcca ctgcagttgc cgcgctcggg gaacggaaac
      841 tctctgcagt gatccacgtg cctgacaccg cgatagatgc agaagagccg gtaaagatta
      901 ctctctacac cctccagaac gatatccagt ccaccatagt gaacgtgaaa ttaaaggagg
      961 tcttcctgga ctacgaggag gagctccgtc aggtgcgctc cggcaggctc gatctcattc
     1021 ccatccccct ggagttccct gcaagcaggg cagggcccgg tttctacgag ttcatctatg
     1081 gtctcttgat accccttctc ctcttcatgc cggccatcat atccgcggcg ctcatcatcg
     1141 acctcatcac cgaggagtat cagcaccaca ccctcgagac cctcctctcc accccgatga
     1201 ccatggcaga ggtggtgtgg ggcaaggtcg cggcctgtgt cgtgctcgtt ccattgcagg
     1261 ccgggacatg gatccttctc cttgcactca accgcatcca tatccagaac attctccaga
     1321 ttctggccca cgtgatctcc ttttcactcg tgctggtcct tatcggggcc ctcactgccc
     1381 tttattacag ggagaggacc gccgcccagt tcatattctc gaccaccgtg gtggtggtga
     1441 tgctcgtcgt gctggcattc cccggcaacc cgctgaacct gattgcactg cttgcaaccg
     1501 gcgcagcggg gatgggacaa tggagcgttc tcggggtgac tctccttttc tgctgcggcc
     1561 tcggccttct cacccacgtg tatgccgggc gggtctccac ccgggcgccg ggtgagtagc
     1621 cgccacaggg tttttccccc aattttgggt cgcgaacaga aaaaaacgac aaaatgcact
     1681 ttatttgctt tttcttccct gttgagacag agtatttcga tacattgaag tcactcgcat
     1741 cgattactac cattacgaag aaggagcggg aaggatgtac tgtcccaagt gcggaaggga
     1801 gacggagagc ggggggagat actgccagtg gtgcggtgcg gatacgcgcg tcgcgccgcc
     1861 aagggctgtc ctccggaaga aagtcggaga tatcagcact gagaaatatg ccggcctggg
     1921 gagaaggatt ggttctggtt tgattgatat cctcttcctc atcttatttg acatgatagc
     1981 ggtcggcatg atctccatca tctcgtggtt actgcagagg ccctgcccaa tctccgagtc
     2041 catcgtgatg ctctaccagt attaccgcca tgtcccccgc acagatgcgt acggaaatgt
     2101 cgtgaacgcc gccatcccct cccagatcgt cttttccgcc ggaatactct ttcttttgat
     2161 tccctggatc tacttcgcgt ggcttgaaag ctccagaaac caggcgacgc tcgggaaaat
     2221 ggtggcacga atcgccgtga ccgatatgca ggggagccgt atcacttttg cacggggaac
     2281 gcttcgtttc ttcgcgatgt cgctctcagt cctcacactc tgcgtgggat tcatcatccc
     2341 ggccttcacc cgttacaagc agggactgca cgacattatc gcagggacac tgatgttccg
     2401 gcaggaccca taacatccag tgtcccctcc aggaaccccc atacggaacc tggtgcggga
     2461 ctcatatcat catgcgcgcg atgcaaaaca caatttttcc gggtatccac gcgatgaccc
     2521 ggtatgaggg acaggtgaga atgaaacaag ggtgtttcgg catggcatgc cggtgatacc
     2581 gcatggctgc gtcctccccg ctctccagac tctcccttcg ctcttctctc atactgtgta
     2641 tcaccgcgat catcctcatt gtgatcatgt tcctgatggc cgccgcatat gcgcaggcct
     2701 attcatggac ggtgcaacag gatcacgagc aggcgctgtt caccgagatg tacctggaag
     2761 agtcgattct tctcgctgac aggggcttaa gcctctacga cagtacgctt gattcccgga
     2821 tggaggaggc gttcctgggg tacctgtcgg catatcacgc atcagaaggt gacatcagcc
     2881 gcattgacct gcccgccata cgggacgccc tggattccgg ctttcggggg cagctcgacc
     2941 tctacattat aaacgagagc ggggttatag tcgcgtccac ggtccccgga gttatgtatc
     3001 tcgattttaa aaattacccc gaatttttcc gctccatcac ccgtatccgt gaaggcgacg
     3061 aattcgtagc cgacagggtg gtccgctctg tcgtgaacac gacggaaggc accgtctccg
     3121 ggcagctccg gaaatttgct ttttatccaa cgcccgatca ccgctacctt ttggagatgg
     3181 gccttgtcat ggagggattc gagaaccagc ggtcggaact ctcctatgtc gaggtgacga
     3241 gacggataca ggagatgaac ccatccatcg aactgatacg gatctttgac gtaaatggaa
     3301 acctcctcct ggaacggggg gtcaaccccc acacgggtgc aaaccagaca ttcattggag
     3361 agatcttttc gaccgggaag gacgcggaga tcctggagca ggatcggaac cggcgcctcc
     3421 attatatcta tgttgacctt cgcgagtcat caaccgcgtc tgatatgagc ctagtcgcag
     3481 agatcaccta tacccaggaa ccactgcgca aggcactctc acagatcctc ctctattaca
     3541 ccctcattgg gttcattgcc atcgtaatgg gtatcgtcat ggccta
//
LOCUS       DAOQVJ010000119         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_29636_length_3580_cov_4.769078, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000119 DAOQVJ010000000
VERSION     DAOQVJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29636_length_3580_cov_4.769078"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PKL26_03510"
     CDS             complement(<1..105)
                     /locus_tag="PKL26_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82797.1"
                     /translation="MKKARVFVDGALIGLVEDPKALVSQVRAMRRQGVI"
     gene            complement(102..1661)
                     /locus_tag="PKL26_03515"
     CDS             complement(102..1661)
                     /locus_tag="PKL26_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HNS82798.1"
                     /translation="MIDRSVLSKAYFSQEHVARHQLDSFNNFLLQNLQKVVNEQRVIE
                     TDIEIRGRSNDPVWVELGRVEVKKPLVREADGSQGDLFPSEARLRNLTYAAPIHLELT
                     LVQGGERQEPVVTTIGQLPIMVGSAACNLSGMSQEERVSHGEDPLDPGGYFIVNGTER
                     VLMTLEDLASNKIMTEYTERYNERIYVAKVFSQYRGYRALVVVERNRKNLLEVSFPSV
                     AGHLKFVDLMRSLGMVSDHDVVSAVSTDEDILTYMMQNLEESDCDSVEAGVMYVGKKL
                     APNQTKDYQRKRAEFVLDNYLLPHLNYLMPTNLKEGEEGYSEAVAEVRLAKAHFLGRM
                     AEACFDLVLSKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDIKYQLERASMRH
                     RDLSIGTAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRVDHMSVLSHLRRVISPLS
                     RSQPHFEARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISKGVQDEDEVIRVLYT
                     LGVEHIKGDVR"
     gene            complement(1682..1933)
                     /locus_tag="PKL26_03520"
     CDS             complement(1682..1933)
                     /locus_tag="PKL26_03520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HNS82799.1"
                     /translation="MSLKLNVLDHVMVPDHQIMSEDEVRELYSLYTISSEQLPKIYHD
                     DPAVKVIGAKLGDVIRIVRTSHTAGRAESYRLVIKRPKK"
     gene            complement(2011..2084)
                     /locus_tag="PKL26_03525"
     tRNA            complement(2011..2084)
                     /locus_tag="PKL26_03525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2048..2050),aa:Lys,seq:ttt)
     gene            complement(2200..2655)
                     /locus_tag="PKL26_03530"
     CDS             complement(2200..2655)
                     /locus_tag="PKL26_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS82800.1"
                     /translation="MERTILLVDDNKFIVEGLRAILKKRGYQILVSNNGLEALELLES
                     TIPDLILLDISMEPIDGWETLERARGRPGCAQIPVIIFSARKNLGEEARTNNLRVFEI
                     LSKPINTGLLVDAIERALAGRPTSGPEEIKPNTSAFHINIGFEKHISED"
     gene            2779..>3580
                     /locus_tag="PKL26_03535"
     CDS             2779..>3580
                     /locus_tag="PKL26_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82801.1"
                     /translation="MNQISAWLANNSLPFCPESSLALNATRHLSKAERAKLFSPDLEK
                     MRTAEGRWYEAIIYELFVEISKNTDAISHLALKGADAPRGGRNARLGQNGIYYSRSGD
                     ITIRGNGQDLAEFDLLMVDGDHQVTFAEVLTSPSDLKEFEAEIEYKRRLLGYLFDQPK
                     VPFLMVASFNVSNFSAGRRILKTPNTIHLQTATCEDIKSGLRGRQRPPAGWKPGLPHS
                     KMVRASDFFLKRDFDYQKFHDWQRNWVFSSVSNEVDVKSAASPHETSIL"
BASE COUNT          765 a         1044 c          921 g          850 t
ORIGIN      
        1 tatgaccccc tggcggcgca ttgcccgcac ctgggagacc agcgcctttg ggtcctccac
       61 gagcccgatc agggcaccgt ccacaaacac ccgtgccttc ttcatctgac atcccccttg
      121 atgtgctcca ctccaagggt atagaggacc cggatcacct catcctcgtc ctgaactccc
      181 ttgcttatct ccaccatctg ggcaaagttc ttcaccaggc cgcagtttgg accctcgggg
      241 gtctcgctcg ggcagatccg cccccactgg gtggggtgaa ggtcacgtgc ctcgaaatga
      301 ggctgcgagc gggagagggg ggatataacc ctccggaggt gtgagagcac gctcatgtga
      361 tcgaccctgt ccagcagctg ggatacccct gtcctgccac ccacccagtt tccagtcgca
      421 agcgggtgga gaaggcgttc ggtcagcacg tcggcacgga ctgccgtccc gatagagagg
      481 tcgcggtgac gcatgcttgc cctctcgagc tggtacttga tgtcgcgggt gagcctgttg
      541 agtgagatgc ggaagagatc ctccatcagg tctcctgcaa gcttcagccg cttgttggag
      601 taatggtcct tgtcgtcaat cctcctcttc gagaggacca ggtcgaagca tgcctctgcc
      661 atgcgcccca ggaaatgggc cttcgcaagg cggacctctg caacggcttc actatacccc
      721 tcttcgccct cttttaggtt cgtgggcatg aggtagttca ggtgcggcag gaggtagttg
      781 tcaaggacga actccgcacg cttcctctgg taatccttcg tctggttggg cgcaagtttc
      841 ttgcccacgt acatgactcc cgcctcgaca ctgtcgcagt cgctctcctc gaggttctgc
      901 atcatgtacg tgaggatgtc ctcgtcggtt gagacggcgc tcaccacgtc atggtcgctg
      961 accatcccga gtgaacgcat caggtccacg aacttcaggt gtcccgcaac cgaagggaat
     1021 gagacctcga ggaggttctt ccggtttcgt tccaccacca cgagtgccct gtagccccgg
     1081 tattgtgaga agacctttgc cacgtaaatg cgctcattgt agcgctccgt gtattcggtc
     1141 atgatcttgt tcgatgcgag atcctcgagg gtcatcaata ccctctcggt cccgttcacg
     1201 atgaagtacc cgccggggtc aagcgggtcc tccccgtgcg aaaccctctc ctcctgactc
     1261 atcccagaga ggttgcaggc agccgaacct accataatgg gcagctgccc aatggtagta
     1321 acgaccggct cctggcgttc acccccctgg acaagcgtca gctccaggtg aattggtgcg
     1381 gcataggtga ggttgcggag ccttgcctcg cttgggaaga gatccccctg ggatccgtcc
     1441 gcttctctca cgagaggctt cttcacctca accctgccca gttctaccca caccgggtcg
     1501 ttgctccgcc cgcgaatctc gatgtcagtc tcgatgacgc gttgttcatt gaccaccttc
     1561 tgcaggttct gcagaagaaa attgttaaaa gagtcgagct ggtggcgcgc cacgtgctcc
     1621 tgggaaaaat atgccttaga taatacgctc ctgtcaatca gaacgatcag ccccgcctga
     1681 attatttttt cggtcttttg atgaccagcc ggtacgattc ggcccttccg gccgtatggg
     1741 atgtccggac gatacggatt acgtccccca atttggctcc gattaccttc acagcaggat
     1801 cgtcatggta gatttttggc agctgttcgc tggaaatagt gtagagagaa taaagttccc
     1861 ggacttcatc ttcactcata atctggtgat ccggaaccat tacatggtcc agtacattca
     1921 gttttaaact caccccaaac ccccgtcctt tggatgatct gttgctcgca gggacatcac
     1981 cggtaaaacc gctggctgtg accgcggcag cgggcccgga gggatttgaa cccccgacca
     2041 cctggttaaa agccaggcgc tctacctaac tgagctacga gcccaccttc ttctaacaca
     2101 cagcattatt acaatgcaag aaaactatat aaagatattt gtaaaatttg cgctttctcc
     2161 tgcccacgct gcccctcgcg aatgttacac gttctctgtt cagtcttctg atatatgctt
     2221 ctcaaaacct atattaatat gaaaagcgga tgtatttggc tttatttctt cggggccaga
     2281 agtcggtctt ccggccagtg ccctctcaat cgcgtccacc agcaacccgg tgttgattgg
     2341 tttggataat atctcaaaga cccgaagatt attcgtacgg gcttcttctc caaggttctt
     2401 tcttgcggag aatatgatca cgggaatttg agcgcacccg ggtctgcccc tcgcccgttc
     2461 aagagtttcc cacccgtcga tcggctccat ggagatatca agaaggatga ggtcagggat
     2521 ggtgctctcc agcagctcaa gtgcctccag cccgttattt gagacgagga tctggtaccc
     2581 tctcttctta aggatcgccc tcagcccttc cacgatgaac ttgttatcat ctacaagcag
     2641 aatcgttctt tccatggctt tccgctacca ggaccggata cgacatacgc agccattacc
     2701 cgccagcccg caatgtgtgg ttagttatcc tgattgggta tattttttaa ccaaaaaggt
     2761 tatataaatg tataaagaat gaaccaaatc tccgcgtggc tggcgaacaa ttcccttcca
     2821 ttctgccccg agtcatccct ggcactgaat gccacgaggc acctttcaaa ggccgagcgg
     2881 gcgaagctct tctccccgga ccttgagaag atgcgtaccg ccgaggggcg ctggtatgaa
     2941 gccatcatct acgagttgtt cgttgagatc tcgaaaaata ctgatgccat cagccacctg
     3001 gccctgaaag gtgccgatgc accacgcggg ggcagaaatg cacgactcgg gcagaacggg
     3061 atctattatt ccaggtcagg agatatcacc atccgcggca acggccagga cctggccgaa
     3121 ttcgacctcc tgatggttga cggggatcac caggtcacct ttgcagaggt cctgacctcc
     3181 ccctcagatc tcaaggaatt cgaggcggag atcgagtaca agcggagact cctcggctac
     3241 ctttttgacc agccgaaggt cccgttcctc atggtcgcat cattcaatgt ctcgaacttc
     3301 tcggcaggga ggaggatcct caagactccc aatactatcc atctccagac tgccacgtgc
     3361 gaagatatca agtccggtct caggggaaga cagagacctc ctgcagggtg gaaaccgggg
     3421 ttgccgcatt caaagatggt tcgtgcatcc gatttttttt taaaacgcga ttttgattac
     3481 caaaaattcc acgactggca gaggaactgg gtgttttcga gtgtttcgaa tgaagtggat
     3541 gtgaagagtg ccgccagccc gcacgagacc tcgatcctgg
//
LOCUS       DAOQVJ010000120         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_30003_length_3550_cov_3.897568, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000120 DAOQVJ010000000
VERSION     DAOQVJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30003_length_3550_cov_3.897568"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="PKL26_03540"
     CDS             <1..109
                     /locus_tag="PKL26_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HNS82802.1"
                     /translation="SRKYCPRCGPGIILADHPDRAACGKCGYTEFKKPA"
     gene            201..1793
                     /locus_tag="PKL26_03545"
     CDS             201..1793
                     /locus_tag="PKL26_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HNS82803.1"
                     /translation="MNRRHTPVPGQVLGIEGTAWNLSAALFEDDLVDLYSKPYQPPQG
                     GIHPREAAQHHASEMRGVIGRVMKDPSRVSGIAFSMGPGLGPCLRTVATAARALALSL
                     RVPLVGVNHCIAHVEIGRFATGCDDPVVLYASGANTQVIGYLNGRYRIFGETLDIGLG
                     NALDKFARSRGLPHPGGPAIEELATRGSYLDLPYTVKGMDLAFSGLVSAAKEQDAPME
                     DICYSLQETAFAMCVEVTERAIAHSGKDEVLLVGGVGVNRRLQEMLSVMCSERGATLH
                     VPPPKYMGDNGAMIAYTGKIMLEAGCAMDPEETRVNPGFRSDQVEVTWREDDQTTKTG
                     PEVSAKLPKARGAEAVVTFIGDSVEKRRVRKPYRQGTLDTRLIAERTRAEARLISMAR
                     RCGVPTPVIRDATPDTLVMEKVEGRLLKETLCEADLMEAGRTVGRLHAAGIVHGDLTT
                     SNMIMRDSRCVLIDFGLSAVSGEVEARGVDIHVLFQTLESTTDTYPELRQAFCEGYRS
                     AFAGANEVLAREQEISQRGRYL"
     gene            1790..2356
                     /gene="rdgB"
                     /locus_tag="PKL26_03550"
     CDS             1790..2356
                     /gene="rdgB"
                     /locus_tag="PKL26_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNS82804.1"
                     /translation="MKFAVVTGNHHKAREIAQFFAGVAEVEHISLECPETRDDDVGVI
                     SAKKAEYAYGTLHRSLMTDDTAFCIPALGGFPGPYAAYVQKTIGNQGIIRLMEDETDR
                     SAYFETAVAFADEQGISVFRGRIHGRIVSPRGKEGFGYDPVFEWEGRTLAEISLEEKS
                     RVSHRARALANLRDWLEGRERERSHLPG"
     gene            2417..2563
                     /locus_tag="PKL26_03555"
     CDS             2417..2563
                     /locus_tag="PKL26_03555"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HNS82805.1"
                     /translation="MARFPEAEAKLLNVKICMRCNARNAMRATRCRKCGYQNLRPKNK
                     ERKA"
     gene            complement(2579..3229)
                     /locus_tag="PKL26_03560"
     CDS             complement(2579..3229)
                     /locus_tag="PKL26_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HNS82806.1"
                     /translation="MSAIDPDTLPDVACGLLFHLLNRELQDQGIFLFSLVEERIDFRD
                     RFLGLFGRFCGDYPELGEALLHKYGSVDAVYELICQGEGVMPGKTTTMHWIVQDAPDV
                     HPDAIEDELAGKWLIFLPPEEVDPAWTIVRDATCRNELGISAKVSTARPNPDSRDNRK
                     VIYVYTADWQDEADVMRVRERLRELGFADRLGYKRNIETYKGEYSEKGKKVTFYSA"
     gene            complement(3261..>3550)
                     /locus_tag="PKL26_03565"
     CDS             complement(3261..>3550)
                     /locus_tag="PKL26_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314486.1"
                     /note="in Methanocaldococcus jannaschii this enzyme is
                     involved in conversion of
                     5-phospho-D-ribose-1-pyrophosphate to
                     ribulose-1,5-bisphosphate using NAD as a cofactor; part of
                     RubisCO pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HNS82807.1"
                     /translation="AVDATGHDAVLARLVEQKGGDVKVRGEGFMWADRAESEIVSHTR
                     EVFPGLVVCGMAANAVAGEHRMGPVFGGMLLSGERAAALATSSLRQENSAA"
BASE COUNT          742 a         1035 c         1119 g          654 t
ORIGIN      
        1 ctccaggaag tactgcccca ggtgcggccc tggcattatc cttgccgacc acccggaccg
       61 ggcggcgtgc ggcaagtgcg ggtacaccga gttcaagaag cctgcatgag aatgccggag
      121 gcattttcat tggggtaaca ccacgtgccc tgattctctc tcttggcatc acgtgacata
      181 caaccccccg gcatatttcc atgaacagga gacacacgcc tgtccccgga caggtcctgg
      241 ggatcgaggg tacggcctgg aacctcagcg ccgctctttt tgaagacgac ctcgttgacc
      301 tctattcaaa gccctatcag cccccgcagg gagggattca cccacgcgaa gcagcgcagc
      361 accacgcctc ggagatgagg ggggtgatcg gcagggtgat gaaggacccg tcgagagtct
      421 cggggatcgc attctccatg ggtcccggcc tcggcccgtg cctgcgcacc gtggcgactg
      481 cggcccgggc actcgcactc tcgcttcggg tgccgcttgt gggggtgaac cattgcatcg
      541 cgcacgtgga gatcggcagg tttgccaccg ggtgcgacga cccggtggta ctctatgcga
      601 gcggtgcgaa tacccaggtg atagggtacc tgaacggccg ttaccggatc ttcggggaga
      661 ccctggacat cggccttggc aacgcgctcg acaagttcgc gcggagcagg gggcttcccc
      721 accccggcgg tcctgcaatc gaggaactgg caacaagagg ctcctatctt gaccttccct
      781 acacagtcaa ggggatggac ctcgcgttct cgggccttgt gagcgcggca aaggagcagg
      841 atgcccccat ggaggatatc tgctacagcc tccaggagac ggcgtttgcg atgtgcgtgg
      901 aggtgacgga gagggcaatc gcgcactcgg gcaaagacga ggtgctcctg gtgggaggag
      961 tgggtgtgaa caggcgtctg caggagatgc tctcggtcat gtgcagtgag aggggtgcaa
     1021 cgctccacgt gcctccgccg aagtacatgg gggacaacgg cgccatgatc gcgtatacgg
     1081 ggaagatcat gctcgaggcg ggctgtgcca tggaccctga agagacacgg gtgaaccccg
     1141 gttttcgttc ggaccaggta gaggtcacct ggcgggagga tgaccagacg acaaaaactg
     1201 gaccggaagt atccgcgaaa ctcccgaagg cacgcggggc cgaggcggta gtcacattta
     1261 ttggagattc cgtcgagaag cgccgcgtgc gaaagcctta ccgccaggga accctggaca
     1321 cccggctcat cgccgagagg acaagagccg aggcgcgcct tatatcgatg gcccggcggt
     1381 gcggagttcc gacacccgtg ataagggacg caacccccga taccctggtg atggagaagg
     1441 tggaggggag gctcctgaaa gagactcttt gcgaagcaga tctcatggaa gccggcagga
     1501 cggtagggag actccatgct gccgggatcg tccatggcga cctgacgacc agcaacatga
     1561 tcatgaggga ctcccggtgc gtgctgatcg atttcggcct ttccgcggta tccggggagg
     1621 tcgaggcacg cggtgttgac atccatgtcc tcttccagac tctggagagc acgactgaca
     1681 cctaccccga actgaggcag gcattctgcg aaggataccg ctccgcattc gccggtgcaa
     1741 acgaagtgct ggcgcgcgaa caggagatct cacagagagg gcgatacctg tgaagttcgc
     1801 ggtggtgacg gggaaccacc acaaggcccg cgagatcgcg cagttctttg cgggtgtggc
     1861 agaggtcgag catatctccc tcgaatgccc tgaaaccagg gacgacgatg taggagtaat
     1921 ctcggcgaag aaagcggagt acgcatatgg caccctgcat agatccctga tgacagacga
     1981 tactgccttc tgcatccccg cccttggcgg attcccgggc ccctacgccg catacgtcca
     2041 gaaaaccatc gggaaccagg ggataatccg cctcatggag gatgaaacgg accggagcgc
     2101 gtattttgag accgccgttg cgtttgcaga cgagcagggg atctcggtct tccggggcag
     2161 gatacacggc aggatcgtgt cccccagggg taaagagggc tttgggtacg accctgtctt
     2221 cgagtgggag ggaaggacgc tcgccgagat ctccctggag gaaaagagcc gagtctcgca
     2281 ccgggcgcgg gcacttgcga atctcaggga ttggcttgaa ggaagggagc gggaaaggtc
     2341 ccaccttccc gggtgacaca caatcttttt gtgcctgcag cccctttatt taaagaccaa
     2401 atagtggtgt tctgaaatgg cacgatttcc ggaagctgaa gcaaaactgc tcaatgtgaa
     2461 gatatgcatg cgatgcaatg ccagaaacgc catgcgcgca accaggtgcc gcaagtgcgg
     2521 ctaccagaat ctccggccca agaacaagga aaggaaggca taatagaggc ctggctcttc
     2581 aggctgaata gaacgtcact tttttgccct tttcgctgta ttcccccttg taggtctcta
     2641 tgttcctctt gtaccccagg cggtccgcaa acccaagctc gcggagtctc tcccggaccc
     2701 gcatcacgtc agcctcgtcc tgccagtcag cagtgtagac gtagataacc ttccggttgt
     2761 cccttgagtc agggttcggc cgggcggtgc tcactttcgc ggatatcccg agctcgtttc
     2821 ggcaggtggc gtcccggact attgtccagg cagggtccac ttcctcgggg gggaggaaga
     2881 taagccattt cccggcaagc tcgtcctcga tggcatcggg gtggacatcg ggggcgtcct
     2941 ggacgatcca gtgcatggtg gtggtcttcc cgggcattac cccctctccc tgacagataa
     3001 gttcgtagac cgcatccacg ctcccgtatt tatgtaggag cgcctcgcca agttccgggt
     3061 aatcgccgca aaagcgcccg aaaaggccca ggaaacggtc acgaaagtcg atccgttcct
     3121 ctaccaggga gaagaggaat atgccctggt cctgcagttc ccggttgagc aggtgaaaga
     3181 gaaggccgca ggccacgtcc gggagggtgt ccggatcgat tgcgctcata cagtagggtt
     3241 gccggaaacg ggaaaaaagg ttatgctgcg gaattctcct ggcggaggga cgaggtggcg
     3301 agggccgcag cccgctcgcc tgagaggagc atgcccccga agaccggccc catccggtgc
     3361 tcccctgcaa cggcatttgc tgccattccg cacaccacca gtccggggaa gacctctctc
     3421 gtgtgggaaa cgatctcaga ctctgcgcga tcggcccaca tgaacccttc tcccctgacc
     3481 ttcacgtcac cccctttctg ctccacgagc ctggcaagga ccgcgtcgtg ccctgtggca
     3541 tctacggcca
//
LOCUS       DAOQVJ010000121         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_30033_length_3547_cov_4.671535, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000121 DAOQVJ010000000
VERSION     DAOQVJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30033_length_3547_cov_4.671535"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="PKL26_03570"
     CDS             complement(<1..1752)
                     /locus_tag="PKL26_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS82808.1"
                     /translation="MNVIVQPQKGNYKILFLDNLQVRAVGIAEIDATPKGYRPGHYRV
                     RWGGKHQFRSTPSKELVVSLRKASVYLTQADEAFEGFLHDFQIPFTRTSVCRICLMED
                     RVTPLNRKNGIRYTTGEQICFDCARRELRRELATMGKVGMQALGHLEELLRNYRDMDK
                     VLASVQPEKVKISQALFDRLDAHTVQKTQSLDELPLPRAFIDAAGVETLMPAQQMAVD
                     AGLLYGKHLLVVSATASGKTFIGEMAGMKNYLEGRGHTLFLVPLVALAVQKYQRFEER
                     YGKIAGVGVLIGQARIRLPENAPVGDRNIRAPIVVGTYEGVDHFIRSGKRLPPIGTVV
                     IDEVQMLEDPDRGHRLDGLVARLKYLAPKAQFLYLSATIGVPKVLAKKLGATLVRYDD
                     RPVALERYLIFVEHKQKIPTIKRLCTQEYSRVSSKGFHGQTIVFTHARARTHVIADAL
                     GPGYAAYHAGLTSQERREIEAKFLSGKIKAVVTTAALGAGVDFPASQVIFDSLAMGIS
                     WLSVQEFNQMAGRAGRPDFHDLGKVVVLAEPGASYSRESRVTEEEVAMQLLKGEMEEV
                     APEHDLEQSSEEYVANAV"
     gene            complement(1749..2558)
                     /locus_tag="PKL26_03575"
     CDS             complement(1749..2558)
                     /locus_tag="PKL26_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNS82809.1"
                     /translation="MTEASKDPLSSILRSKREVTRLQILVEIAEHQPAVRQQEIADKL
                     GVTPQAVSEYIRELVDEEMAAAHGRGRYSVTRTGIEWILNHAEALEAYARHIRRDIIQ
                     QVSTWTAIAAEDLIEGDQVGVYMEHGWLYASRESRSAMGTVVMDAKAGEDVGVARLSG
                     LIDHTEGVVHVCKVPRVQRGGSRKVRKDRLLEVIEGVRVVAAVGLEGYVALQQAGRTP
                     DMFFGAREGVIEAAFHGIDCAILVVDEEFTDFLKRLEGVGLSYILHDLIAS"
     gene            complement(2542..3054)
                     /locus_tag="PKL26_03580"
     CDS             complement(2542..3054)
                     /locus_tag="PKL26_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS82810.1"
                     /translation="MSGHIRIVNDPVDLVPLLITFHNATYKQVYDLLNKSWMTEEEIC
                     EQLAEGDVGQCLLVLKKGSLVEEKWRMPAPGQKPVKEYRASYNKFRANFQCNFGDLSD
                     LLFIAVSNDEHLRCMVEKVEDELRSGNTSINDIARKFGVNPVFIKGLAKRTPHLDVKG
                     QGLVLLDRSQ"
     gene            3103..>3547
                     /locus_tag="PKL26_03585"
     CDS             3103..>3547
                     /locus_tag="PKL26_03585"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNS82811.1"
                     /translation="MNGKRRSGRIQIRGIVQGVGFRPFVYATATRLGIRGFVRNRGSE
                     VEIQAEGDQFEAFLEEVSRGPPLSRIDRVEVLQDPGPFPGDFSIQPSGSGSLSGMIPP
                     DIATCDQCLQDIHTAGGRYSGYWCTSCVNCGPRYSIIRDLPYDRER"
BASE COUNT          644 a         1129 c         1051 g          723 t
ORIGIN      
        1 gaccgcattt gccacgtact cctcggaact ctgctcgaga tcatgctccg gggcaacctc
       61 ctccatctcc cctttcagaa gctgcatcgc cacctcctct tcggtaaccc tggattcacg
      121 ggagtaggaa gcaccgggtt cagcgagcac caccaccttc ccgaggtcat ggaagtccgg
      181 acggcctgca cggccggcca tctggttgaa ctcctggacc gagagccagg agatccccat
      241 cgcgagggag tcgaagatca cctgcgaggc ggggaaatcg acccccgcac cgagcgcagc
      301 ggtggtgacc acggccttga tctttccgga gaggaatttt gcctcgatct cccgccgctc
      361 ctgggaggtg aggcctgcat ggtaggctgc atatcccggc cccagggcgt cggcgatgac
      421 gtgtgtccgg gcccgggcgt gggtaaagac gattgtctgg ccgtggaagc ccttcgagga
      481 gacccgcgag tactcctggg tgcagaggcg ctttatcgtc gggatcttct gcttgtgctc
      541 gacgaaaatg aggtagcgct cgagggcgac gggacggtcg tcgtaccgga cgagcgttgc
      601 acccagtttc tttgcgagca cctttgggac cccgatggtt gcggagaggt acaggaactg
      661 ggccttcggg gccaggtact tcagcctcgc gacgaggccg tccaggcggt ggccccggtc
      721 cgggtcctcg agcatctgga cctcgtcgat gacaaccgtc ccaatcggcg ggagccgctt
      781 ccccgaacgg atgaagtggt cgaccccctc gtaggtccct acgacgatgg gagctcggat
      841 gttccggtcc ccgacgggag cgttctcggg cagcctgatc ctggcctggc cgatcaggac
      901 ccctaccccc gcgatctttc cgtaccgctc ctcgaagcgc tggtacttct gcaccgcgag
      961 cgcaaccagt gggacgagga agagggtgtg gcccctcccc tccaggtaat tcttcatacc
     1021 cgccatctcg ccgatgaagg tcttgccgct cgcagtggcc gagacgacca ggaggtgctt
     1081 tccatagaga aggcctgcgt cgaccgccat ctgctgggcc ggcatcagcg tctcgacgcc
     1141 ggcggcatcg atgaacgccc gcgggagggg aagttcatca agagactggg tcttctgtac
     1201 cgtgtgggca tccaggcggt cgaagagtgc ctgggagatc ttcaccttct cgggctgaac
     1261 gctcgcgagg accttgtcca tgtcgcggta gttccgcagc agctcctcga ggtgcccgag
     1321 agcctgcatg cccaccttgc ccatcgtggc aagttccctt ctgagctccc tgcgggcgca
     1381 gtcaaaacag atctgctcgc cggtcgtgta gcgtatgccg ttcttcctgt tcaggggggt
     1441 gacacggtcc tccatcaggc atatcctgca tacggaggta cgggtaaagg ggatctggaa
     1501 gtcgtgcagg aacccttcaa atgcctcgtc cgcctgggtc aggtacaccg atgccttccg
     1561 cagggaaacc accagctcct ttgaaggggt gctgcggaac tggtgcttgc ccccccacct
     1621 cacccggtag tggcccggcc gatatccctt cggagtggcg tcgatctccg cgatccccac
     1681 cgcccgcacc tgcaggttgt caaggaaaag gatcttgtaa tttcccttct gcggctggac
     1741 gatgacattc atgaggcgat caggtcatgg aggatatacg agagccccac tccttcaagc
     1801 cgtttcagga aatcggtgaa ctcctcgtct acgacgagga tggcacagtc gatgccatgg
     1861 aatgccgcct cgatcacccc ttctcttgcc ccaaagaaca tatctggcgt ccttcccgcc
     1921 tgctggaggg ctacgtagcc ctcgaggccg accgccgcca ccacccgcac gccctcaatg
     1981 acttcaagga gccgatcctt cctcaccttg cgtgatccgc ccctctggac gcgggggacc
     2041 ttgcatacat ggaccactcc ctcggtgtgg tctatcaggc ccgagagccg ggcaactccc
     2101 acgtcctcgc ctgctttcgc gtccatcacc accgttccca tggcactccg ggactcccgc
     2161 gatgcataca gccagccgtg ctccatgtac acccccacct ggtcaccctc gatgaggtcc
     2221 tcggcggcaa tcgctgtcca cgtggagacc tgctggatga tatcccttcg aatgtgccgg
     2281 gcgtaggcct ccagcgcctc ggcatggttg aggatccact cgatcccggt cctcgtgacg
     2341 ctgtacctgc cgcggccatg ggcagccgcc atctcctcgt ccacgagctc ccggatgtac
     2401 tcggagacag cctggggcgt caccccgagc ttgtccgcga tctcctgctg ccttactgcc
     2461 ggctggtgct cggcgatctc gaccaggatc tgcagacggg tcacttcgcg cttgcttcgg
     2521 agaatagacg acagggggtc cttactggct tcggtcaagg agaaccagcc cctgtccctt
     2581 tacgtcgaga tggggggtcc gcttcgccag tcctttgatg aagacggggt tgaccccgaa
     2641 ctttcgtgcg atatcgttga tagaggtatt ccctgaacga agctcatcct cgaccttctc
     2701 gaccatgcag cggaggtgct cgtcgtttga gactgcgatg aagaggagat cggagagatc
     2761 cccgaagttg cactggaaat ttgccctgaa cttgttgtag gaggcacggt actccttcac
     2821 cggtttctgc ccgggggctg gcatcctcca cttctcctcg acaaggctcc ctttttttag
     2881 caccagcagg cattggccta cgtccccctc ggccagctgc tcgcagatct cctcctcggt
     2941 catccaggac ttgttcaaca gatcgtagac ctgcttatag gtcgcattat gaaacgtgat
     3001 aagcagagga acaaggtcca ctggatcgtt gactatcctg atatggccgc tcactggtaa
     3061 aaccctagta ttatatggcg tgataaatca ataaatcttt gtttgaacgg caagcgcagg
     3121 agcggaagga ttcaaatccg tggaatcgtg cagggtgtcg gcttccggcc attcgtgtat
     3181 gccacggcga ccaggctcgg gatccggggt tttgtcagga accggggcag cgaggtcgag
     3241 atacaggccg agggcgacca gttcgaggcg ttcctggagg aggtctcgcg tggaccccca
     3301 ctctcccgaa tcgaccgggt ggaggtactc caggacccag ggccattccc aggcgatttt
     3361 tcgatccagc caagcgggag cggttccctc tccggcatga ttccgccgga catcgcgacc
     3421 tgtgaccagt gcctccagga tatacacacc gcaggagggc ggtattccgg gtactggtgc
     3481 acctcctgcg tgaactgcgg cccgcggtac agcattatcc gcgacctccc ctacgacagg
     3541 gagagga
//
LOCUS       DAOQVJ010000122         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_30263_length_3529_cov_4.565343, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000122 DAOQVJ010000000
VERSION     DAOQVJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30263_length_3529_cov_4.565343"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKL26_03590"
     CDS             <1..302
                     /locus_tag="PKL26_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNS82812.1"
                     /translation="CYLQVHAPCCTGWGFEGEKTIAIGKLAIETGLWVNYEMEDGKVT
                     RVKKVVRKPVETYLKEQRRFRHLFKPARNDAEIAKVQAVADRNAERFGIDIKLKK"
     gene            complement(412..1026)
                     /locus_tag="PKL26_03595"
     CDS             complement(412..1026)
                     /locus_tag="PKL26_03595"
                     /inference="COORDINATES: protein motif:HMM:NF039237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HNS82813.1"
                     /translation="MRASHGDRCRFALVALCLAALLTILPAAALGAEYRITQNGTSYR
                     AALDLVDAERFEFHEIGMLGERIPLQVSDVALSGDCNPCEFEQSGNAAITFQRGNYTL
                     FFQGPVRDNHFVATFESPRRVSVLLPPGLDVRNPALGMISQGGIVTDSGVEGIIVTWN
                     STRTAEVRFYDEGRENLLYIFANFWIIIAVVLLVPFLLTWKRRE"
     gene            complement(960..2168)
                     /gene="thrC"
                     /locus_tag="PKL26_03600"
     CDS             complement(960..2168)
                     /gene="thrC"
                     /locus_tag="PKL26_03600"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNS82814.1"
                     /translation="MYRLVCVECGESYPPAEILYHCTRCGHLLAVEYDLESAHVDRAA
                     WDHRPLSVWRYREILPVSIPPVSLHEGGTPLYQLSRIGEEIGLPHLYAKHEGMNPTGS
                     FKDRGMTVGVSMALQLGKTSVACASTGNTSASLAAYAAKAGIPAVVLLPAGKVALGKV
                     AQALMHGARVLSIRGNFDRALEMVHELCLSHGLYLLNSVNPFRLEGQKTIGFEALDQL
                     GEVPDRMVLPVGNAGNISAVYKGFTELASLGLMDRIPMMTGIQASGSSPVVRAIHEGL
                     PELIPEANPETVATAIRIGAPVNAEKALTAIRKTGGTAESVTDEEILAMQRELARKEG
                     IGVEPASAASVAGVKKLREMGVIDRDEKIVCVVTGHLLKDPETVIRQCEPPMEIDADL
                     PSLLSALQRS"
     gene            complement(2302..3108)
                     /locus_tag="PKL26_03605"
     CDS             complement(2302..3108)
                     /locus_tag="PKL26_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS82815.1"
                     /translation="MKLFCAQVTSVWEDPVATLKRVEPCIARASRGGGDLICFPEQFA
                     TGWDPGSSQHVQGIDGAITGRLCSLAQEYSIAILGSFREKTKGNPRNTSVVISSEGEI
                     ISTYAKCHLFSPASEDRHYQQGNTIATFPVGGIECGLAICYDLRFTSLFRLYADAGVH
                     AVLVPAAWPEARLQHWELFIRARALEYQVYVAGINTTGINPIDIYAGCSMVADPTGGV
                     IVRAGPGEALISCELDREYVERVRSALPVTRDRRDDLSPAQETKRQGKAG"
BASE COUNT          692 a         1013 c         1110 g          714 t
ORIGIN      
        1 cctgctacct gcaggtgcat gctccctgct gcacggggtg ggggttcgag ggggagaaga
       61 ccattgcaat aggaaaactt gcaatcgaga cggggctctg ggtcaattac gagatggagg
      121 acggcaaggt gacaagggtg aagaaagtgg tgagaaagcc cgtggagacc tacctgaagg
      181 aacagcgccg cttccgccac ctcttcaagc cagccaggaa cgatgccgag atcgcgaagg
      241 tccaggcggt tgccgaccgg aacgcggagc ggttcgggat cgatatcaag ctgaagaaat
      301 aacacaactt tttttgtatc cccaaacccc gtgtatattt cctgcgtatg ccttcccgga
      361 caggcaactg ccccggaata tcccttcttc cttgacggat taccggggac cctactctct
      421 cctcttccag gtgaggagga acgggacgag caataccaca gcgatgatga tccagaaatt
      481 cgcaaagatg tagagcaggt tctccctgcc ttcgtcatag aaccggactt cggcagtcct
      541 tgtggaattc caggtgacaa tgatgccctc cacgccagaa tcagtcacga tacctccctg
      601 gctgatcatg ccgagggcgg gattccttac atcgagcccg ggagggagta agacgctgac
      661 ccgcctggga gactcaaacg tggcgacaaa gtggttgtcg cggacaggcc cctggaaaaa
      721 gagggtataa ttgccccgct ggaatgtgat ggcggcgttt ccgctctgct caaattcgca
      781 ggggttgcag tcgccggaga gcgcaacatc cgacacctgc agggggatgc gctcgccaag
      841 catcccgatc tcgtggaact cgaaacgctc tgcgtccacg agatcaagcg ctgcgcgata
      901 cgaagtcccg ttctgggtga tgcggtactc tgcaccaagc gcggctgccg gcaggatcgt
      961 caggagcgct gcaaggcaga gagcaacgag ggcaaatctg catcgatctc catgggaggc
     1021 tcgcattgcc tgatcaccgt ctccgggtct ttcagcaggt gcccggtcac cacgcataca
     1081 atcttctcgt cacggtcgat cacacccatt tcacggagtt tctttacccc tgcaacggag
     1141 gccgccgagg cgggctcgac cccgatccct tcttttcgtg cgagttcccg ctgcatggca
     1201 aggatctctt cgtcggtcac ggactcggcg gtcccgccgg tcttccggat tgcggtcagt
     1261 gccttctcgg cattcaccgg tgccccgatt cggatagcgg ttgccacggt ctcgggattt
     1321 gcctcgggaa tcaattcggg cagtccctcg tgtattgccc tgacgacggg gctcgatccg
     1381 gatgcctgga tcccggtcat catcgggatg cggtccatca gtccgaggga tgcgagttcg
     1441 gtgaatccct tgtagacggc ggagatgttg cctgcgtttc cgaccgggag caccatccgg
     1501 tccgggacct ccccgagctg gtcgagtgcc tcaaacccga tggtcttctg cccctcgagg
     1561 cggaaggggt tgacggagtt gaggagatag aggccgtggg agaggcagag ttcgtgcacc
     1621 atctcgagcg cccggtcgaa gtttccgcgg atggagagta cccgtgcacc gtgcatcagt
     1681 gcctgtgcga ctttcccgag cgccactttc ccggccggga gcagcacgac tgccggtatg
     1741 ccggcctttg cagcataggc ggcaaggctt gccgaggtgt tgccggtact cgcgcaggcg
     1801 acggaggtct tgccaagctg cagcgccatg ctcacgccga ccgtcatgcc acggtccttg
     1861 aacgaaccgg tgggattcat gccctcgtgc ttggcataga ggtgtggaag ccctatctcc
     1921 tccccgatac gtgaaagttg gtagagagga gttccccctt cgtggagcga gacaggggga
     1981 atggagaccg ggaggatctc ccggtaccgc cataccgaga gcggtctatg gtcccaggca
     2041 gcccggtcca cgtgtgcgga ttcgaggtca tactccaccg cgaggaggtg gccgcagcgc
     2101 gtgcagtgat ataatatctc ggcaggcggg tatgattccc cgcactctac gcacacgaga
     2161 cggtacatga atacatgtgt gcatccggag gggaaaaagg aggtgcacgc ggcggaaacg
     2221 gtgcccggaa cctgctgaaa agtcgcgaac gggagaaaag aaatctcctt tcgttttccg
     2281 gcaatggatc gactgccccg gtcagcctgc cttcccctgt cgtttcgtct cctgtgccgg
     2341 tgagaggtca tcccgcctgt ccctggtgac ggggagcgcg gatctcaccc gctccacgta
     2401 ttcccggtcg agttcgcagg atatgagtgc ctctcccggg cctgcacgga cgatgacgcc
     2461 gccggtcggg tcggccacca tggagcagcc ggcatatata tcgattgggt tgatgccggt
     2521 cgtgttgatc ccggctacat agacctggta ttcgagtgca cgggcgcgga tgaagagttc
     2581 ccagtgctgg agccgtgctt ccggccaggc cgcaggtacc agcaccgcgt ggacgccagc
     2641 gtctgcgtac agccggaaga gtgaggtgaa acgaaggtcg tagcagatgg cgagcccgca
     2701 ctcgatcccg ccgacaggga acgtggcgat ggtattcccc tgttggtagt gccggtcctc
     2761 tgacgctggg gagaagaggt ggcacttggc gtaggttgaa ataatctctc cctcggaaga
     2821 gattacgacg gacgtgttcc gcggatttcc cttggttttc tcgcggaaag agccaaggat
     2881 cgcgatggaa tactcctgcg cgagcgaaca cagtcttccc gtgatggccc cgtcgatccc
     2941 ctgcacgtgc tgggacgacc cggggtccca cccggtggcg aactgctcgg ggaagcagat
     3001 gagatcgcca ccgccccgcg acgcccttgc gatgcagggc tccactctct tcagggtggc
     3061 caccgggtct tcccagacgc ttgtgacctg ggcgcagaag agcttcatac catttggtgt
     3121 ggggggcggg aatattttga tatttctggt gttgccggga tggctgcccc ttttccccgg
     3181 gcgacctgtg ggtctcattg aatcacggtt ttcaccatcg gggaggggga aatatccact
     3241 tcttcacgat acgcgatagt ctgtgggcag ggtaacaacc ggacagattg gatagtactt
     3301 gcggcttcgc actgtgccta tcgcgcttag gggatgccac agtggcgggg cgaagcccca
     3361 agaacgtcat cgtgagggga gtgcggtctg tttctcttga aacagtttga ctggaacctg
     3421 caaccggagt ggggagaatc ccccctccag acctcccctt ctatatgcga tagcctgtgg
     3481 gcagggtaac aaccggacag attggatagt acttgcggct tcgcactgt
//
LOCUS       DAOQVJ010000123         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_30701_length_3494_cov_3.744112, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000123 DAOQVJ010000000
VERSION     DAOQVJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30701_length_3494_cov_3.744112"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..969)
                     /locus_tag="PKL26_03610"
     CDS             complement(49..969)
                     /locus_tag="PKL26_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNS82816.1"
                     /translation="MIYVVGHTAIDHICRVPAFPPRNGSATITERKVYFGGGAANIAA
                     GIARLGEPCTLVSAIGGDFPGSEYDRWQKSLGLNQRFFEVPDAHTATAFMFTDEDGDQ
                     ITFFEWGASRIFSDSEAPALPFVHMATADPDFNTRVAEKAAFSTFDPGQDLHRYSRTQ
                     LDAILSHINILFANQHEVESMCRTLGLSREGLAARVPLAVFTMGAGGSEYFHGEECAH
                     IPAIRVRMADPTGAGDAYRAGFLAAFRRGYYPRDCCRIGTVTASFAVEVTGCQNNLPS
                     WEEMETRFRQVFGDVPVIEPGAGEAQRSRV"
     gene            complement(966..1304)
                     /locus_tag="PKL26_03615"
     CDS             complement(966..1304)
                     /locus_tag="PKL26_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HNS82817.1"
                     /translation="MPDYRVILEGAWVVKDVSSLEDAVSIAISEAGKRLNPVAAYVEV
                     EVGMLACPFCEGELNSALVVARTALVGLLLEMKVFRAESPEHATRIARSVIGKALRDV
                     PLEVKEVCAL"
     gene            complement(1315..1842)
                     /locus_tag="PKL26_03620"
     CDS             complement(1315..1842)
                     /locus_tag="PKL26_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS82818.1"
                     /translation="MDSSEFLQFLVSRCSVREYDTREVPDADLTYILDCASTAPSAGN
                     LEAWDVVIVDGQEEKERVAEASFGQEHVVSAALLLVVCANYVRSMSRYGDRGVLFAIQ
                     DATIACTYMMLAAHARRLHTCWVGSFQDDEVRDMLDLPSHVRPIAILCVGKGFSPGSF
                     TERMPVVEHVHHQSW"
     gene            complement(1829..2185)
                     /locus_tag="PKL26_03625"
     CDS             complement(1829..2185)
                     /locus_tag="PKL26_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82819.1"
                     /translation="MDLKKAVKGYQFAERAKSELIISSQLTIALAGFPESERAGGKRM
                     LVLLLEAVRSELEFAWKGTEMSDFRRAINLLSESISTVESENYGAASLKISESISAVT
                     TAAQGSWQVLEAHGLI"
     gene            complement(2274..2603)
                     /locus_tag="PKL26_03630"
     CDS             complement(2274..2603)
                     /locus_tag="PKL26_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HNS82820.1"
                     /translation="MGKTGSVQWTQVKGVKGQIRLVPKSEGELKKPGPNQRFKAGASL
                     RKLEQLTGEGQGGFRGGRGGRGGRGGRGGRGGRSSESGPQANPMVRKAMRRPKVSVLG
                     AKQKSGR"
     gene            complement(2675..>3494)
                     /locus_tag="PKL26_03635"
     CDS             complement(2675..>3494)
                     /locus_tag="PKL26_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNS82821.1"
                     /translation="DTVGAEESDCVVLVAGTPRQAGCACQQIIRRAELALLGVPEETR
                     KMLEGGSTSYMRPLPGAARMYPETDVLPVTISPEYWEDIPRVELLQAKAERYMTAHGL
                     DAAVAKQVAYSEYLPLFERSVGLGIPATLSARTLIATLKELSRDGLDIRPLVADCDAA
                     GNAVMPAVLQVLKGVVEERVGKEAIPDVLRRIVQGESAETAMQAVEATVAGTDLDSLA
                     RRIVSERMTFVSEKGMGALGPLMGVMMKEVGGAVDGKKVSQVLKAALDQALSGR"
BASE COUNT          666 a         1122 c          973 g          733 t
ORIGIN      
        1 ccccaaccgc tcgatcttca actccccccc catcactgac atgtccactc acacccggga
       61 acgctgagct tctcctgccc cgggctcaat cactggaaca tccccgaata cctgccggaa
      121 cctcgtctcc atctcctccc atgaggggag gttgttctgg cacccggtga cttcgacggc
      181 aaaagaggcg gtcaccgtcc cgatacggca gcaatcccgg gggtagtatc cccgcctgaa
      241 cgccgcgagg aaccctgcac ggtaggcatc ccccgcgcca gtaggatctg ccatcctgac
      301 gcgtatcgcg gggatatggg cgcactcttc tccatggaag tactcgcttc ctcctgcccc
      361 catcgtgaag accgccagag ggacgcgggc ggcgaggccc tcccttgaga ggccgagagt
      421 ccggcacatg ctctccactt cgtgctggtt cgcaaagagg atattgatgt gggaaagaat
      481 ggcgtccagc tgggtacggc tgtacctgtg caggtcctgc cccgggtcga acgtggagaa
      541 ggctgccttc tctgcgacgc gggtgttgaa atccggatcg gccgtggcca tgtggacgaa
      601 agggagggca ggggcctctg agtcggaaaa gatgcgggac gctccccatt caaagaacgt
      661 gatctggtcc ccatcctcgt ccgtgaacat gaacgcggtg gcagtgtggg catcgggaac
      721 ctcgaagaac cgctggttca aaccaagcga tttctgccat cgatcatact cgctgccggg
      781 aaaatctccc ccaatcgcgc tcacgagggt acagggctcg ccgagcctgg cgatgccagc
      841 ggcaatattg gctgctcccc cgccgaagta taccttccgc tcggtaatgg tggcagaccc
      901 gttcctgggc gggaaagcgg ggaccctgca gatgtggtcg atggcggtat ggccgacgac
      961 gtatatcaca gtgcgcagac ctccttgact tccaggggga cgtcgcggag ggcttttcca
     1021 atcacggatc tcgcgatgcg ggtggcgtgt tcgggcgact cggcacgaaa gactttcatc
     1081 tcgaggagga gcccgacaag ggcggtgcgc gctaccacca gcgcactgtt cagttcacct
     1141 tcgcagaacg ggcaggcgag catgcccacc tccacctcca cgtaggctgc taccgggttc
     1201 agcctctttc ctgcctcgct gatggcaatg ctcaccgcat cctcgagcga tgagacatcc
     1261 ttcaccaccc atgcgccttc aagtattaca cgataatctg gcattcctga tcccttacca
     1321 tgactggtgg tgcacgtgtt caaccaccgg cattcgttct gtaaagctcc ctggcgaaaa
     1381 gcccttccct acgcagagga ttgcgatcgg ccggacatgg gacggaagat cgagcatatc
     1441 ccgcacctcg tcgtcctgga acgagccgac ccagcacgta tggagccttc ttgcatgagc
     1501 cgcgagcatc atgtaggtgc aggcaatggt cgcgtcctgg atggcaaaga ggacacccct
     1561 gtcgccatat cttgacatgg atctcacgta attcgcacat actacaagga gcagggcggc
     1621 agatacaaca tgctcctggc cgaaagaggc ctctgccacc ctctctttct cctcctgccc
     1681 gtccacgatg acaacgtccc aggcctcgag gtttcccgca cttggggcgg tgcttgcgca
     1741 gtccaggata taggtgaggt cggcgtcggg cacttcgcgt gtgtcgtatt ccctcaccga
     1801 gcagcgcgac acaaggaatt gaaggaattc agatgagtcc atgtgcttcg agaacctgcc
     1861 acgacccctg ggctgcagta gtgactgcac tgatggactc gcttattttc agggacgccg
     1921 ccccgtaatt ctcgctctca accgtgctga tcgattccga gaggaggttg atggcacgcc
     1981 ggaagtccga catctcggtc cctttccagg caaattcaag ttctgaacgg acggcttcaa
     2041 ggaggagtac gagcatccgt tttccgccgg cgcgttctga ttcagggaac cctgcgagtg
     2101 cgatggtgag ctgcgaggag ataatcagtt cactcttcgc ccgttcggcg aactggtaac
     2161 ctttcaccgc tttcttcaga tccatacttt cactgtactg cagggataca agatacttct
     2221 gtgatctaag tcaaatggcc aaaaaaaaat tgagaatcgg agatatattg tggttatctg
     2281 ccagatttct gcttggcgcc gagcaccgag accttgggcc tccgcatggc tttcctgacc
     2341 atggggtttg cctgcggacc ggactctgaa gagcgcccgc cgcgcccgcc acgcccgcca
     2401 cggccaccgc gtcctccacg gccaccgcgg aaacctccct gtccctcacc ggtcaactgc
     2461 tcgagcttcc ggagcgaggc tcccgctttg aaccgctggt tcggccccgg tttcttcaat
     2521 tctccctcag actttggcac caggcgtatc tggcccttga cgcctttcac ctgcgtccat
     2581 tgtacggatc ctgtttttcc cataggcaaa ctccttaatt tgtttgcgat gtggtctttt
     2641 aatactttgg acctctcccg tgcggaatga gaggtcacct gcctgaaagt gcctggtcta
     2701 gcgcggcttt caacacctgg gagacttttt tgccgtccac cgcgcccccc acttccttca
     2761 tcatcactcc catcagggga ccaagggcgc ccatgccctt ctcactcaca aacgtcatcc
     2821 tctcggatac gatgcgtctt gcgagtgagt cgaggtcggt ccctgcaact gtcgcctcga
     2881 cggcctgcat ggctgtctct gcggattctc cctggacgat ccttctgaga acatcgggga
     2941 tagcctcctt gcccactcgt tcttccacga cccctttcag cacctgcagg actgccggca
     3001 tcaccgcgtt gccagccgca tcgcaatcag ccacgagtgg gcggatgtcg agcccatccc
     3061 gcgagagttc cttcaaggtt gcaatgagag tccgggcaga gagcgtggcg gggatgccga
     3121 gccccaccga cctctcaaag agtgggaggt actcggagta tgccacctgc tttgccaccg
     3181 ccgcatcgag gccatgcgcc gtcatatacc tctccgcctt tgcctgcagg agctcgacac
     3241 gggggatatc ctcccagtat tcaggggaga ttgtaacggg gagcacgtcg gtctcggggt
     3301 acatcctcgc ggccccgggc agcggccgca tgtacgaggt gcttcccccc tcaagcatct
     3361 ttctcgtctc ttcggggacc ccgagaaggg ccagttccgc ccttcggatg atctgctggc
     3421 aggcgcaccc ggcctgtcgg ggggtcccgg ccaccaggac gacgcagtcc gactcttctg
     3481 caccgaccgt atcc
//
LOCUS       DAOQVJ010000124         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_30706_length_3493_cov_4.795230, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000124 DAOQVJ010000000
VERSION     DAOQVJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30706_length_3493_cov_4.795230"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PKL26_03640"
     CDS             <1..1135
                     /locus_tag="PKL26_03640"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HNS82822.1"
                     /translation="INEMEMDEVEHLTRAMVDTGERLTFHAHPIVDKHSIGGVPGNKI
                     SLLVVPIIAASGLKIPKTSSRAITGAGGTADLMEVLAPVDFSASEVQRMTEKVGGVIV
                     WGGATNIAPADDKIIIQEYPFRIDARGQMLASVMAKKYAVGADLVVIDIPVGTHAKVA
                     TIQEGRKLAREFIELGDRLNMQVECALTYGDSPVGRSIGPNLEVMEALRILEGGESPG
                     SLLQKSVAIAGMALEMSGKAARGMGAQMAEEILKKGDALKKFRQIIEIQGGDPKIRSD
                     DIEPGKYQFVVNAPTSGYVIELNNRSLISLARTAGAPHDRGAGVLLHAKKGSRVEKGQ
                     PLFTIYADRNWRLQKALEEGRQLMPVLVEGMLLDRVPHDTRWS"
     gene            1299..2690
                     /locus_tag="PKL26_03645"
     CDS             1299..2690
                     /locus_tag="PKL26_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNS82823.1"
                     /translation="MQRIGTCFAFALLAMLLMAGPASGVTVLVNIYEKEGNITPLPGA
                     ALYTNNALVGKSDSGGNIEFSHPGTEIIQIRVEKLGYAPWSGDIGLNATTLLVEMTKK
                     KVPLTVQVFDTDALAPVAGASVSISDGAGKNTTVTDANGTTMFTVSGDSAYNLEITAP
                     NYRTFTDHLEIARDQKTVQVMLVRDDRFSVLVKDETSGAPLPGAAVIIDGVERGITDA
                     KGVVTLSLPREKVYQVHVRIEGYDDYHERMNVGRDQALVTTLLRKSPYSVFISVYNEE
                     RDPVEEASVFLDGQPVGSTNRYGRLQLANLSFGEYALEVRHPGFETHRQQMQVRVQGE
                     DITVDLAFPAANLSIAALEGGDVPVHGATIFIDGKEQGTTGENGTLPVRLRLNTLYVI
                     SAEKEGFNPVKLEKTVSSRNETAPVFISMERSIDWVLVIVVLAVIVAGCMGAFLIMKK
                     RGQARGRSRRGGL"
     gene            complement(2771..2842)
                     /locus_tag="PKL26_03650"
     tRNA            complement(2771..2842)
                     /locus_tag="PKL26_03650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2807..2809),aa:Arg,seq:tcg)
     gene            2901..3338
                     /gene="dcd"
                     /locus_tag="PKL26_03655"
     CDS             2901..3338
                     /gene="dcd"
                     /locus_tag="PKL26_03655"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNS82824.1"
                     /translation="MILSSSEIERRLRGEDMSGDLVIEPYSEECQQPASYDLRVGGDF
                     VLPPRVCTLVNTLERVELPSDLAATMRCRSSFGRRGVILTAGFVDPGFRGQLTLCLVN
                     MGTEHLTLRKNDRVVQIIFHQVTDGKNAYSGRYQDSTGVIKAR"
     gene            3339..>3493
                     /locus_tag="PKL26_03660"
     CDS             3339..>3493
                     /locus_tag="PKL26_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HNS82825.1"
                     /translation="MIRTERKYIEILRILKEHREPMGAKRLSELMAERGFVLSDRAVQ
                     YYLRYLD"
BASE COUNT          817 a          936 c         1072 g          668 t
ORIGIN      
        1 catcaacgag atggagatgg acgaggttga gcacctcacc agggcgatgg ttgacaccgg
       61 ggagcggctg acgtttcatg cccacccaat tgtcgacaag cattcaatcg gaggggtacc
      121 tggaaacaag atctcactcc tggtggtccc cataatcgcc gcgagcggtc tcaagatccc
      181 aaagaccagc tcgagggcca tcaccggtgc gggcgggacg gccgatctca tggaggtcct
      241 cgccccggtc gatttctcag catcagaggt ccagaggatg acagagaagg tgggcggagt
      301 gatcgtgtgg ggcggcgcca ccaacatcgc gcccgcagat gacaagatca tcatccagga
      361 gtatcccttc cgcattgatg cgagggggca gatgctcgca agtgtcatgg ccaagaagta
      421 tgcggtcggt gctgacctcg tggtgatcga catcccggta ggcacacacg ccaaggtggc
      481 cacaatccag gaggggagga aacttgccag ggagttcatt gagcttgggg accgcctcaa
      541 catgcaggtt gagtgtgccc tcacatacgg ggactccccc gtgggacgga gtatcgggcc
      601 gaaccttgaa gtaatggagg cgctgcggat ccttgagggc ggagagagcc cgggctctct
      661 cctgcagaag agcgtcgcga tcgcgggaat ggcgctcgag atgtccggga aggctgcaag
      721 gggaatgggg gcacagatgg ccgaagagat cctcaaaaaa ggcgatgccc tgaaaaaatt
      781 ccgccagatc atcgagatcc agggaggaga cccgaagatc aggtctgatg acatcgagcc
      841 cgggaaatac cagttcgttg tcaatgcccc tacgtcaggg tacgtcatcg agctgaacaa
      901 caggtccctc attagcctcg cgaggacggc gggagcgccc catgaccgtg gggcgggcgt
      961 cctcctccat gcaaagaaag gcagccgggt cgagaaaggc cagcctctct tcacaatcta
     1021 tgctgacagg aactggaggc tgcagaaagc gctcgaagaa gggcggcagt tgatgccggt
     1081 acttgtcgaa gggatgctcc ttgaccgggt gccgcacgac acccggtgga gctgaacctg
     1141 ccggtccttc aacttccagg aggagccatc cctgtcaatg ccgcacctgc gggttccctc
     1201 ctggtgaatc cggaaatcca tccctttttt ttcctattcg gaaagccggg cagcgcgtta
     1261 ccaatcaaat gatatacaga gagcgccgat atcagaatat gcaaaggatt gggacctgtt
     1321 ttgcatttgc actcctcgcc atgctgctta tggcagggcc ggcctccggg gttacggtcc
     1381 ttgtcaatat ctatgagaag gaagggaaca tcacccccct gccgggtgca gcactgtaca
     1441 caaacaatgc actcgtggga aagtcagata gcggagggaa catcgaattt tcccaccctg
     1501 gaacggagat tatccagatc agggtcgaaa aactgggata tgcgccctgg agtggcgata
     1561 tcgggctcaa cgccacgact ctgctcgttg agatgaccaa aaagaaggta cctctgaccg
     1621 tccaggtctt tgataccgac gctcttgcac cggtggcggg ggcaagtgtg agcatcagtg
     1681 acggtgccgg gaagaacacg accgtcacgg acgcaaacgg tacaaccatg ttcaccgtct
     1741 cgggcgactc ggcgtacaac ctcgagataa ctgccccgaa ttaccgcacc ttcaccgacc
     1801 acctcgagat cgcgcgtgac cagaagaccg tccaggtaat gctcgttcgt gacgaccggt
     1861 tctctgtcct tgtgaaggac gagacaagcg gggccccgct ccccggtgcg gcagttatca
     1921 ttgacggcgt cgagaggggt atcaccgacg caaagggggt tgtcacgctg tccctgcccc
     1981 gggagaaggt ctaccaggtc cacgtgagga tagagggata cgacgattat catgagcgga
     2041 tgaatgtggg cagggaccag gcccttgtca ccaccctgct cagaaagtcc ccgtacagtg
     2101 tgtttatctc ggtctacaac gaggagcggg accctgtcga ggaggcatcg gtcttccttg
     2161 acggccagcc agtcggctcc accaaccgtt acgggcggct gcagcttgca aacctctcgt
     2221 ttggggagta tgctctcgaa gtacgccatc ccgggtttga gacccacagg cagcagatgc
     2281 aggtacgcgt gcagggtgag gacatcactg tcgaccttgc gttccccgct gcaaacctct
     2341 cgatcgctgc cctcgagggg ggagatgtcc cggttcatgg ggccactatc tttatcgacg
     2401 gaaaagagca gggaacaacg ggtgagaatg gcacccttcc agtccgcctt cggctgaaca
     2461 ctctctatgt aatatcagcg gaaaaagagg gcttcaatcc cgtgaaactt gagaagaccg
     2521 tctcgtcccg gaatgagacc gcacccgttt tcatctccat ggaaaggagc atcgactggg
     2581 ttcttgtcat agtggtgctg gccgtaatcg tggcaggatg catgggagca ttcctcatca
     2641 tgaaaaagag gggccaggca aggggaagga gtcgaagggg cggactctga aaccttcacc
     2701 gattgtgtca tatgtacagg ggtatcaggt gccaggcgag gggaaggagt cgtgttggag
     2761 aggagaaaag cggacccagc gggaatcgaa cccgcgtcct tgggtccgaa gcccaagagg
     2821 atatcctcta ccccatggat ccgtcacagg cgttggcagg acaacatatt aagcattttc
     2881 tgtagtattg atctgtaaca atgatcctgt cgtcatcaga gatcgagaga cgccttcgcg
     2941 gagaggacat gagcggagac ctggtcatag agccgtatag cgaagagtgc cagcaacccg
     3001 cgtcgtacga cctcagggta ggaggtgatt ttgtcctgcc accccgggtc tgcaccctcg
     3061 tgaataccct ggagagggtt gaactgcctt cggaccttgc ggccacgatg agatgccggt
     3121 catcctttgg caggagaggg gttatcctca ctgcaggttt cgttgaccct ggtttccggg
     3181 gccagttgac actctgcctg gtcaacatgg gtacagagca cctcaccttg cgaaagaacg
     3241 acagggtagt gcagatcatc ttccaccagg tcaccgatgg gaagaatgcc tacagcgggc
     3301 gttaccagga cagcacaggg gtcatcaagg cgagataaat gattcggaca gagcgaaaat
     3361 acatcgagat cctgagaata ctcaaggagc acagggagcc gatgggtgca aaacgcctct
     3421 cggagttgat ggctgagagg ggttttgtcc tgagcgaccg tgcggtgcag tattatcttc
     3481 ggtaccttga cga
//
LOCUS       DAOQVJ010000125         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_31011_length_3469_cov_4.120094, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000125 DAOQVJ010000000
VERSION     DAOQVJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31011_length_3469_cov_4.120094"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKL26_03665"
     CDS             <1..972
                     /locus_tag="PKL26_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HNS82826.1"
                     /translation="WLIGTQVPHPLVEITGARGKTTTAHALAHLMEGPGILHTSLGTT
                     RYPSRENFSKTGITPASLIDAASFAHSRSGWLIAEVSLGFTGAGTLGILASMETYRCA
                     GGKKDALEEKLRSGSALPLVLTPPGTGRCQNRLPADEIARVKDDGCQYAWEGIRGEFK
                     NPLLEHAAYRDPLALAAAAACILGIDPACLSTFSPVAGRLALSWTGKTAIIDDSNSGT
                     CAMTAAQAIRFARHATGRAGPLTLVIGKEEGSICEGFPGEAIVDLIQAECPDRVILVG
                     REYGSLIPGSLRRKIHISCCNSLADGEALALDDGDAGMVVLAVKTWR"
     gene            978..2042
                     /gene="cfbD"
                     /locus_tag="PKL26_03670"
     CDS             978..2042
                     /gene="cfbD"
                     /locus_tag="PKL26_03670"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HNS82827.1"
                     /translation="MKYMQPRPSSIVAALYTARDLGVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADHEFIFGGQQPLEAVLRYAEETFSPRRIAVVGTCVSMIIGEDLQSAI
                     RNAGISTPTISVEIHAGFPENIDGVIATLEPAAAEGWISEDELERQKRLLASANEVER
                     LRGAASRPYIEPSRGDLKHLAAERLLALARSGRKGVAIMNAKKETAYMFADELLALHQ
                     ACPGAAVTYLANLEERGLPKVREDARRIREELCEKGLLPVLSGALDEYGANGDTLGEK
                     IRALSPDFAFIVGVPHAIPPRYLEGVECISVTNGPRQVEPLRDQGHRYVVVEVDLHPR
                     TLGVTHLVESELGAVIRSIA"
     gene            2039..3421
                     /gene="cfbB"
                     /locus_tag="PKL26_03675"
     CDS             2039..3421
                     /gene="cfbB"
                     /locus_tag="PKL26_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HNS82828.1"
                     /translation="MKALMIAGDRSGSGKTSITLAIASLFARKMKVQTFKVGMDYIDP
                     SYLTGVTGRPCRNLDTYVMDRDQVRVVYTHGCMGADLALIEGVRGLYEGAEALEETGS
                     SASVARCLDIHVVLVVNARSITRSAAALVRGFQAFDPEIRIAGVILNNVAGDKHIRKA
                     TTAIEHYCGIPVIGAIPRMPEMELGMRHLGLMPYREGQENRKFQEKIRAVTENIGEHV
                     NLDQLLSVACATPPPQHKPDIFKPAKDPDLSIGIAYDEAFNFYYADLFDVLHAGGART
                     VLFSPLHDSLPDADGYILGGGYPELFLPELAARDGLKNDIRDASGLGIPIYAECGGLM
                     YLTEAVILKDGWQGIQGERMYPMCGIFKGTTTMPARRVVAYVEGKSLPGSPMGESHFR
                     GHEFHYSDVSLCPGTRFCYSLSRGAGISGGSDGALMNRTLGCYTHLHPVASLPMFSHF
                     LDACRKPARA"
BASE COUNT          749 a         1029 c         1031 g          660 t
ORIGIN      
        1 tggctgattg gaacgcaagt gccacacccc ctggtagaga tcacaggcgc caggggtaag
       61 acaacaacgg cgcatgcact ggcgcacctc atggaggggc cgggcattct ccatacctcg
      121 ctcgggacca ccaggtatcc ttcaagggaa aacttcagca aaacgggcat cacacctgcc
      181 tcgctcatcg atgctgcatc ttttgcccat tcgcgcagcg gatggctgat cgcagaggta
      241 tcgcttgggt tcaccggggc cgggacactt ggcatcctgg cctctatgga gacctaccgt
      301 tgcgcgggag gaaagaagga tgcacttgag gagaaactcc gttccgggtc cgccctcccc
      361 ctggtcctca ccccgccagg aacgggccgg tgccaaaaca ggcttcctgc agatgagatt
      421 gcgagggtca aagatgacgg atgccagtat gcctgggaag ggatacgcgg agagttcaaa
      481 aatcccctcc tggaacacgc agcatatcgc gatccgcttg cactggcagc cgccgccgcc
      541 tgcatcctcg gtatcgatcc cgcctgcctc tcgacattct ctcctgttgc aggaagactg
      601 gccctctcct ggactggaaa gaccgcaatc atagatgatt caaattctgg cacctgcgca
      661 atgactgcgg cacaggcgat aaggtttgca aggcacgcga ccggacgcgc tggcccgctc
      721 actcttgtca tcggcaaaga agagggatct atctgcgagg gattcccagg agaagccatc
      781 gtggacctga tccaggcgga atgcccggac cgcgtcatcc tggtcggacg tgaatacggc
      841 tcgttgatac caggctcact gcgacgtaag atacacatct cctgttgcaa ttccctcgca
      901 gacggagaag ccctggccct ggatgacggc gatgcgggca tggtggtgct ggcggtgaag
      961 acatggcggt gaatcccatg aaatacatgc agccgcggcc cagttctatc gtagcagccc
     1021 tctataccgc acgcgacctt ggggtcgacg tggcaatcct gcacgggcct tcggggtgct
     1081 ccttcaagca tgcccgtctc ctcgaggagg acgggatgag agtcctcacg acctctcttg
     1141 cggatcatga attcatattc ggaggacagc agccgcttga ggcggttctc cgttacgcgg
     1201 aagagacatt ttctccccga aggatcgcag tggttgggac ctgtgtgtcg atgatcatcg
     1261 gcgaggatct ccagtcagcc atccggaacg cgggcatctc cactcccacg atatcggtcg
     1321 agatccatgc cgggttcccg gagaatatcg atggagtgat tgccaccctc gaacctgcgg
     1381 ccgcagaagg ctggataagc gaagatgaac ttgaacgcca gaaacggctc cttgcatccg
     1441 ccaacgaggt cgagcggctg cgcggtgcgg cgtcccggcc gtatatcgag ccttcccggg
     1501 gtgacctgaa gcatctcgcc gcagaacggc tccttgccct cgcacggagt ggcagaaaag
     1561 gggttgccat catgaatgca aagaaagaga ccgcgtacat gttcgcagac gaactcctcg
     1621 cgctccacca ggcctgccca ggtgcagccg tcacatacct cgcgaacctc gaggaaaggg
     1681 ggcttcccaa ggttcgtgaa gacgcccgcc gcatccggga agaactctgc gagaaagggc
     1741 tcttaccagt tctctctgga gccctggacg agtatggggc caacggggat acgctcggag
     1801 agaaaatacg ggcactctcc ccggactttg cgttcatcgt tggcgttccg catgcaatcc
     1861 ccccccgata cctcgaagga gtggagtgca tctcggtcac gaatggccca cggcaggttg
     1921 aacccctcag ggatcaggga caccggtatg tggtggtcga agtggacctc caccccagga
     1981 cgctcggcgt cacccacctc gtcgagagcg agcttggtgc agtcatcagg agcattgcat
     2041 gaaagcgctg atgatcgcag gcgatcgttc cgggagcgga aagacgagca tcacgcttgc
     2101 aatcgcctcc ctgttcgcaa gaaagatgaa ggttcagacc ttcaaggtcg gtatggacta
     2161 catcgacccg tcctacctca ccggcgtcac cgggcggccc tgccggaacc tggataccta
     2221 tgtcatggac agggatcagg tcagggtggt ctatacgcac gggtgcatgg gggccgacct
     2281 ggccctcatc gaaggagtgc gtggcctcta tgaaggcgca gaggcgctcg aagagaccgg
     2341 gagcagcgcc tcggtggcaa ggtgcctgga tatccatgtg gtgctggtgg tgaatgcgcg
     2401 gagcatcacg cgaagtgcgg ctgcccttgt caggggattc caggccttcg atccggaaat
     2461 ccgtatcgca ggtgtcatcc tgaacaacgt ggcaggagat aagcacatcc gcaaggccac
     2521 gactgcgatc gagcattact gcggcatccc ggtaatcggt gcaatccccc gcatgcctga
     2581 gatggaactc gggatgcgcc acctcgggtt gatgccctac cgcgaggggc aggagaaccg
     2641 aaagttccag gaaaagatcc gggcggtcac agaaaatatc ggagaacatg tgaaccttga
     2701 ccagctcctc tccgttgcat gtgccactcc cccgcctcaa cacaagccgg atatcttcaa
     2761 acccgcaaag gatccggacc tgagcattgg aatcgcctac gacgaggcct tcaacttcta
     2821 ttacgcagac ctcttcgacg tgctgcatgc cgggggggcc cgaaccgtcc tcttcagccc
     2881 ccttcatgat tcgctccctg acgctgacgg ctacatcctt ggcgggggat atcccgagct
     2941 cttcctcccc gaactcgccg cacgcgacgg gttgaagaat gacatccgcg atgcatcggg
     3001 gcttggaatt cccatatatg ccgaatgcgg cgggctgatg tacctcaccg aggccgtcat
     3061 actgaaagac gggtggcagg gtatccaggg tgagagaatg tacccaatgt gcgggatctt
     3121 caaagggacg acgaccatgc ctgcaaggag ggtggtcgcg tacgtggaag ggaagagcct
     3181 tccaggctcc ccaatgggtg agagccattt caggggtcac gagttccatt attccgacgt
     3241 gtctctttgt cccgggaccc ggttttgcta ctcgctctcg cgaggtgccg gtatatcagg
     3301 gggatcagac ggggccctca tgaacaggac gcttgggtgc tacacgcacc ttcatccggt
     3361 ggcaagcctt cccatgttct cccatttcct tgacgcctgc cgaaagcctg cgagggcctg
     3421 attcaatcag caggtaaaaa ataccaggag aataaacatt catttcatg
//
LOCUS       DAOQVJ010000126         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_31059_length_3465_cov_4.991202, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000126 DAOQVJ010000000
VERSION     DAOQVJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31059_length_3465_cov_4.991202"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..762)
                     /locus_tag="PKL26_03680"
     CDS             complement(91..762)
                     /locus_tag="PKL26_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNS82829.1"
                     /translation="MEILSAWWMAFLAGLYAPLGSPCVIPLYPGFLSFLAGRKGERER
                     STLTLGMIVVAGIILSMLVFGIIFVAVVQVSLSRFLSFLSPIAFLVLAAFSIVLILDI
                     DLSRWTGSISQPRSGRPFVDAFLLGLFFGVIILPCNAAAVVALLAIGTTTTGFLANLG
                     SFFAFGLGMSLPLLAFAAVSRTRSREILAWFSRHRRAIHAVAGVVMLGVSLYYLLFVF
                     SPLCG"
     gene            complement(766..1323)
                     /locus_tag="PKL26_03685"
     CDS             complement(766..1323)
                     /locus_tag="PKL26_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS82830.1"
                     /translation="MNVLKFSPVLPPAGLVLATCCIIFSAGCMGTTGPVNGGTMTGET
                     WRTIELRDVQTGETFSVASLSGKPVILYTFTVSCPICTLQQKEISAFKKEHGEDVAVI
                     GIDIDPKEEEAVLKSHIEKNGFLGYYTLSPPGMAGSLTERFGPVVVTPASAPVILVCP
                     GGDARLLGTGIKTSAQLSSLIRAVC"
     gene            complement(1349..2050)
                     /gene="radB"
                     /locus_tag="PKL26_03690"
     CDS             complement(1349..2050)
                     /gene="radB"
                     /locus_tag="PKL26_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HNS82831.1"
                     /translation="MNPPPLSTGSVDLDEILGGGLARGVITQVFGEPASGKSTICVMA
                     SVSALRSGDQVLFLDSEGFSAERFRQIAGEDAEKLAAGLYLYEPEDFTQQGVMVKSLD
                     AMLRGGGTGLVVVDSATGLYRSQLERGRDAMQKLTGQVLMLLGYARRYRIPVLISNQV
                     YTDTTRNVFVGLGGTALEHLSKTIIRLERRDHLRRATLAKHRSLPPGAWFDFEITQNG
                     IRAWEPPHNPKKEGI"
     gene            complement(2047..3234)
                     /gene="larC"
                     /locus_tag="PKL26_03695"
     CDS             complement(2047..3234)
                     /gene="larC"
                     /locus_tag="PKL26_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNS82832.1"
                     /translation="MRVLVFDPFRGAAGDMICGALLHLGAGADLVSRAMESVVAEPSI
                     KEVTRAGIRAVKVDTRAPVLLRSLTEVLERVNGADAPAGAIGMACRVFERIAAGEAVI
                     HGECPHFHEVGADDAIADVVGACTALSSLGVDAVAVGTITVGGGTVRTAHGTVPVPAP
                     ATLAILQGTGLAVQYGTGEDGELCTPTGAALLAEFATAFPGVPPRGRVVATGYGAGER
                     NPPSIPNVLRATLIETSHFSGGDEVDILETNIDDVTGEILAGCVDRLMSVGARDVCAV
                     PCTMKKGRPGYLVRVVCFPGDSARLSVALAEETGTLGVRCIPSIHRFVAERETRIVTI
                     DLSGRRWDVPVKCGTLGGKAFSVKAESDRVFEVARECSVPAREVARLAEAEAWKAAGR
                     MSE"
     gene            3274..>3465
                     /locus_tag="PKL26_03700"
     CDS             3274..>3465
                     /locus_tag="PKL26_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82833.1"
                     /translation="MPELHLKVDSAYPGDQGGGKARLDPETMLFLKISPGDIVVVEGK
                     RRTVAKVWRSLVEDWNQNKI"
BASE COUNT          714 a         1065 c         1078 g          608 t
ORIGIN      
        1 ggcgaagatg gagggccctg cgaccacggt cttttcgggg acgatcacgt tctgatacct
       61 tttttgttcc tcctgtaaga catctcctgt ctacccacag aggggagaga atacgaagag
      121 gagataatag agcgacaccc cgagcattac cacccccgcc acggcatgga tcgctctccg
      181 gtgccgggag aaccacgcga ggatctcgcg gctccgggtt ctggagacgg cagcgaatgc
      241 caggagcggg agggacatcc cgagtccgaa cgcgaagaac gatccaaggt ttgcaaggaa
      301 gcccgtggtc gtcgtcccga tcgcaaggag agccaccacg gccgcagcgt tgcacggaag
      361 gatgatgacg ccgaagaaaa ggccgagcag gaaagcgtcg acgaatggtc tcccgctccg
      421 gggctgtgag atgctcccgg tccaccggct gagatcgata tcgagaatca ggacgattga
      481 gaaggcggcg aggaccagga atgcgatcgg ggagaggaac gagaggaacc gggagaggga
      541 gacctgcacg accgcaacaa agatgatgcc gaacacgagc atggagagaa tgattcctgc
      601 caccactatc atcccaaggg tgagcgtgga gcgttcgcgt tcaccttttc ttcccgcgag
      661 gaacgagagg aaccctgggt agagtgggat gacgcaggga gagccgagcg gggcgtaaag
      721 gccggcaagg aacgccatcc accaggccga gaggatctcc accgctcagc acaccgccct
      781 gatcaacgat gagagctggg ctgaggtctt gatgccggta ccaaggagcc gggcatcccc
      841 gccaggacag accagtatca caggggctga ggcgggtgtc accaccaccg ggccgaaccg
      901 ttcggtgaga gagccggcca ttcctggcgg ggaaagggtg taataaccca ggaaaccgtt
      961 cttttcgatg tggctcttca gaacagcctc ctcctccttt ggatcgatgt caattccgat
     1021 gacagcaaca tcttcgccat gctccttctt gaacgccgag atctccttct gttgcagggt
     1081 gcagatgggg caggagacag tgaaggtgta caggatgacg ggtttcccgg atagcgaggc
     1141 cacggagaat gtctcccctg tctgcacgtc acggagttcg atggtcctcc aggtctcgcc
     1201 ggtcatggtt cctccattga caggaccagt agttcccatg caccctgcgg agaagattat
     1261 gcagcacgtg gccagaacaa ggcctgccgg ggggagaacc ggcgagaact tcagcacgtt
     1321 cataccagta cagctcgaaa atcgggggtt aaataccttc cttcttcggg ttatgcggcg
     1381 gttcccaggc gcggatcccg ttctgcgtga tctcgaagtc gaaccaggct ccaggaggga
     1441 gggagcggtg ctttgcgagg gttgcccggc ggaggtggtc cctcctctca aggcggatga
     1501 tggtctttga gaggtgctcg agggcggtgc ccccaaggcc gacgaagacg ttcctcgtgg
     1561 tgtcggtata gacctggttc gagataagca ccgggatgcg gtacctcctc gcatacccaa
     1621 gcagcatcag cacctgcccc gtcaatttct gcatggcgtc gcggccacgt tcgagctggc
     1681 tccggtagag acctgttgca gagtcgacca ccacgaggcc ggtcccccct ccgcggagca
     1741 tggcatcgag gctctttacc atcacgccct gctgcgtgaa atcctcgggt tcatagagat
     1801 agagtcctgc agccagtttt tcggcgtctt ctcctgcgat ctgccggaag cgctcggcag
     1861 aaaagccctc gctgtccagg aagagcacct ggtctcctga acggagcgcc gagaccgaag
     1921 ccatcacgca gatggtgctc ttcccgctgg ccggttcacc gaagacctgg gtgatcactc
     1981 cgcgtgcaag gccgcccccg aggatctcgt caagatcgac agagcccgtg gagaggggag
     2041 ggggattcat tcactcatcc tgcccgccgc cttccaggcc tctgcttctg ccagccgggc
     2101 aacctcgcgg gcggggactg agcactccct cgccacttcg aacacgcgat cggactcggc
     2161 cttgaccgag aaggcctttc ccccaagggt cccgcatttg accgggacgt cccatcgcct
     2221 gccggacaga tcgattgtga cgattcgtgt ctccctctct gccacgaacc tgtggatgct
     2281 cgggatgcag cgcacgccaa gggtgccggt ctcttccgcg agggccaccg agagccgggc
     2341 tgagtccccg ggaaagcaga ctacccgcac gagatagccc gggcgccctt tcttcatggt
     2401 gcagggaacg gcacatacgt cccgtgctcc cacactcatc agcctgtcca cgcatcctgc
     2461 aaggatctcc ccggtcacgt cgtcgatgtt ggtctccagg atgtctacct cgtctccgcc
     2521 cgaaaaatgg gatgtctcga tgagggttgc acggagcacg ttcgggatgg aaggcggatt
     2581 cctttccccg gcaccatacc cggtggcgac caccctgccc cggggaggaa cgccgggaaa
     2641 cgcggtggca aactctgcaa ggagtgcagc gcccgtggga gtgcaaagct ccccgtcttc
     2701 gcctgttcca tactgcacag cgaggccggt tccctggagg atggcgaggg tcgcaggagc
     2761 cgggacaggg actgtcccgt gagcggtgcg gaccgtcccg cccccgacag taatagtgcc
     2821 gacggcaacc gcatccacgc cgagagagga gagtgcagtg caggccccga cgacatcggc
     2881 gattgcgtcg tccgccccca cctcgtggaa atggggacat tcgccatgga tgaccgcctc
     2941 ccctgcagcg atcctctcga acaccctgca ggccattcca atcgcccccg cgggtgcatc
     3001 tgcgccgttc acccgttcaa ggacctctgt caacgaccgg agcaggaccg gtgcccgggt
     3061 gtccaccttc acggcccgga tccctgcccg tgtcacctct ttgatggagg gttcggcgac
     3121 gaccgactcc atcgcccggg acacgaggtc cgcgcctgca ccaaggtgca gcagcgcccc
     3181 gcagatcatg tcaccggcgg ctccccggaa cggatcaaag accaggactc gcatacgtac
     3241 ttataaatcc cagcgtattg cacctttaaa ttaatgcctg aactccatct gaaggttgac
     3301 agcgcgtatc cgggcgatca gggcggcgga aaggcgaggc tcgacccaga gacaatgctc
     3361 tttttgaaga tctcccccgg cgacatcgtg gtggtggaag ggaaacgccg gaccgtggcc
     3421 aaggtgtggc ggtctctcgt ggaggactgg aaccagaaca agata
//
LOCUS       DAOQVJ010000127         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_31071_length_3464_cov_5.246407, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000127 DAOQVJ010000000
VERSION     DAOQVJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31071_length_3464_cov_5.246407"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKL26_03705"
     CDS             <1..361
                     /locus_tag="PKL26_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS82834.1"
                     /translation="IQQHSVSHPTTFAMYGGVAALSCDQSCVEVMRKEFNRRRDYLVQ
                     ELLLMGFEVAPADGAFYAYVKVEGDDMEIARRWLEEVHVAVTPGSAFHTPGWERISYA
                     TSMENLREAVLRIRRCR"
     gene            393..812
                     /locus_tag="PKL26_03710"
     CDS             393..812
                     /locus_tag="PKL26_03710"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNS82835.1"
                     /translation="MTMRILFVCSRNAGRSQMAEGIARALLPTTCQVFSAGERPSVLN
                     GTAVRVMAEKGVDISRQSSKGLSSLPFREYDVLVVLGEWEDPSRLPPSRRVIREILPD
                     PAAFSRAEGDVLEGFRALRDCLWDRIFAWEREGQFRA"
     gene            981..1430
                     /locus_tag="PKL26_03715"
     CDS             981..1430
                     /locus_tag="PKL26_03715"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNS82836.1"
                     /translation="MKDKMHHRWMDCAVAEARAGLSEGGIPIGAVLVRDGQILGRGHN
                     RRVQEGNPILHAEIDCLQNAGRVGSFRNCVLYSTLMPCYLCAGAAVQFGIPVVVAGES
                     ENFPGARDLLEAKGVQVIDLHREDCISMMKEFITRCPELWNEDIGEL"
     gene            1554..2363
                     /gene="budA"
                     /locus_tag="PKL26_03720"
     CDS             1554..2363
                     /gene="budA"
                     /locus_tag="PKL26_03720"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNS82837.1"
                     /translation="MQDRMVVAVCAVCVAAGFATGFWLAAMPENEIDQEVIYQVSTID
                     ALLQGSCEGTMTFGEVKKHGDFGIATFDSLDGEMIALGGDFYQIKADGQVVAVTDDMT
                     TPFGSVTFFSQDCTFPIRYAANFSDFSRQAEAFLPSRNHMYAIQIRGEFPYVKARSIP
                     RQETPNARLVDAAANQSIFEFHETRGTVVGFWTPDLVGGLNVPGFHLHYITEDRSAGG
                     HILDFVLENATVEMDVTSRFTVVLPTEGSFTEVDLSGDLSHELAVAEKGNA"
     gene            2559..>3464
                     /locus_tag="PKL26_03725"
     CDS             2559..>3464
                     /locus_tag="PKL26_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNS82838.1"
                     /translation="MITIYRGGTDGLERVNKYESGTWVNVVNPTQKEIEELVSWFNIP
                     SDFLTDPLDVDERARIEREEGCILILLRSPRRELPDADIPFTTLPIGIIFTQNVLITV
                     SLNESDVIQEFFTGKVKHFSTDQQTRFVLLLFLRSTLVFMRYLKEINKMTTTVERSLY
                     KALKNEQLIRLLMLEKSLVFFVTSLRSNALMMEKFNTTSCMKMSEEDRDLFEDLIIEN
                     KQAIEMANIYSSILSDMMDAFASVISNNLNVVLKLLTTVTIILMIPTLVASIYGMNVE
                     LPYQHTPYAFLVTVAISLALSAVGII"
BASE COUNT          764 a          949 c         1025 g          726 t
ORIGIN      
        1 gatccagcag cactccgtca gccacccgac cacatttgcc atgtacggcg gggtggcagc
       61 cctctcctgc gaccagtcat gcgtcgaggt gatgagaaag gagttcaacc ggagaaggga
      121 ctaccttgtc caggagctcc tcctgatggg cttcgaggtc gcccctgcgg atggtgcgtt
      181 ttacgcgtac gtgaaggtgg aaggggacga catggagatt gcccgccgct ggctcgagga
      241 ggtgcacgtt gcagtgactc ccgggtccgc gttccacacc ccgggatggg agcgtatctc
      301 ctatgccacc tccatggaga acctccggga ggcagtcctc cgcatccggc gctgccgttg
      361 agggccttcc cttattattc cctctctttt gtatgaccat gaggatcctc tttgtctgct
      421 ccaggaatgc cgggcgttcc cagatggccg aggggattgc gagggcactc ctccccacga
      481 cctgccaggt gttcagcgca ggggagaggc catcagtgct gaacggcacg gcggtgaggg
      541 taatggcaga gaagggagtc gatatctccc gccagtcatc gaaggggctc tcttcactcc
      601 ccttccggga gtacgacgtc ctcgttgtcc tcggggagtg ggaggatccc tcgcgtctcc
      661 ccccttcccg gcgggtgatc agagagatac tccccgatcc ggcggctttt tccagggcgg
      721 aaggagatgt gctggagggg ttccgggctc tccgcgactg cctgtgggac aggattttcg
      781 catgggaacg tgaaggccag ttccgcgcgt agtaaaaagg aaacgttagt caggacagga
      841 gtcccaatcc catcatggaa aacgacccca gggaagatct tcagatggga ggaaccgggc
      901 attacccttg tgttcgccgc ccgtcaggca atccgggaat cagggggaga gtgagaggag
      961 ggtaaaaaag gatggaatac atgaaagaca aaatgcacca ccgctggatg gactgtgcgg
     1021 tggccgaggc cagggccggc ctctccgaag gaggcatccc tatcggggct gtcctggtca
     1081 gagatgggca gatccttggc cgtggtcata accgcagggt gcaggagggc aacccaatcc
     1141 ttcacgcgga gatagattgc ctccagaatg ccggccgggt cgggagtttc cgcaattgtg
     1201 tgctatactc aacgctgatg ccctgttatc tctgtgcggg ggcggcagtc cagttcggga
     1261 tccccgtggt tgtcgcgggc gaatcagaaa attttcccgg tgccagggac ctcctggagg
     1321 caaaaggggt acaggtaatc gacctgcaca gggaggactg catctccatg atgaaggagt
     1381 ttatcacccg ttgccccgaa ctctggaacg aggatatcgg cgaactctga ttcgatcaat
     1441 gcggacagcg tgtccctatg aatcagggct aacaataaca ggtacgcgag gcacgggagt
     1501 gcaatgaaat gagagatatt aaaaaagacg cagcagaagg aggcggaccc tgaatgcagg
     1561 accggatggt agttgcagta tgcgctgtct gcgtagcagc cgggtttgcg accgggttct
     1621 ggcttgcggc catgcccgag aatgagatag accaggaggt catctaccag gtgtccacaa
     1681 tcgatgccct cctgcagggg tcctgtgagg gcaccatgac ctttggcgag gtcaaaaaac
     1741 acggtgattt cggcattgcc accttcgaca gcctcgacgg ggagatgatc gcgctcggcg
     1801 gggacttcta ccagataaag gctgacggtc aggtcgttgc ggttaccgat gacatgacaa
     1861 cgccctttgg gagtgtcact tttttctccc aggattgcac cttccccata agatatgctg
     1921 ccaatttcag tgatttttcc aggcaggcgg aggcgttcct tccctccagg aaccacatgt
     1981 atgcaattca gatccgcgga gagttcccat acgtcaaggc ccggagcatc ccccggcagg
     2041 agacgccgaa cgcccggctc gtggatgctg cagccaacca gtccatattc gaattccatg
     2101 agacgagagg aacggttgtc ggtttctgga cccccgatct tgtcgggggc ctgaatgtgc
     2161 ccggattcca tctccactat ataacggaag atcgatcggc cggtggccac atactggatt
     2221 ttgtccttga gaatgcgacg gtggagatgg atgtcacgtc aaggttcact gttgtcctcc
     2281 cgaccgaggg gagtttcacg gaggtagatc tttcaggcga cctgagtcat gaacttgccg
     2341 tggcggagaa gggcaacgcc taaatccagg gaaaaaagcc tcttttccgg ctattttggg
     2401 gcagaagatc gaaaggtatt ttgcgtatcc gggaaaacga ccagatctag gatcattcgg
     2461 acagttggaa cgggtgaagg gggggaatgc gtgcggggtc gtcttccctg tcccaaaccc
     2521 cgatgattcc tccagcggcc cgggggacgt gggcagccat gataaccatt tacaggggtg
     2581 ggacggatgg gctggagcgg gtcaacaagt acgagagcgg gacctgggtc aacgtcgtaa
     2641 atcccaccca aaaggagatc gaggagctgg tctcctggtt caatatcccc tctgatttcc
     2701 tcaccgatcc cctggacgtc gacgagcgtg cccgcatcga gcgagaggag gggtgcatcc
     2761 tcatcctgtt gcgaagcccg cggagggaat tacctgatgc agacattcca tttaccacgc
     2821 ttcccatcgg gatcatcttc acgcagaacg tcctcatcac cgtgtccctg aatgaatcgg
     2881 acgtcatcca ggagtttttc accgggaagg tgaaacactt ctccacagac cagcagaccc
     2941 gttttgtact cctcctcttc ctgcgcagca cgctcgtctt catgcggtac ttaaaggaga
     3001 tcaacaagat gaccacaacc gtcgagagat ctctttacaa ggccctgaag aacgaacagc
     3061 tgatccgcct gctgatgctg gaaaaaagtc tcgtattctt cgtgacgtcg cttcgctcca
     3121 acgcactgat gatggagaag ttcaacacca cctcctgcat gaagatgagc gaggaggatc
     3181 gcgacctctt cgaggacctg atcattgaga acaagcaggc catcgagatg gcaaatatct
     3241 acagcagcat tctctcggac atgatggatg cgttcgcatc tgtcatctcc aataacttaa
     3301 acgtggtgtt gaagctcctc accacggtga ccatcattct catgataccg acccttgtgg
     3361 ccagcatcta cggaatgaac gtcgagcttc cctaccagca tacgccctat gccttcctcg
     3421 tcacggtcgc gatatccctt gccctttcag cggttgggat catc
//
LOCUS       DAOQVJ010000128         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_31572_length_3425_cov_3.583086, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000128 DAOQVJ010000000
VERSION     DAOQVJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31572_length_3425_cov_3.583086"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..740
                     /locus_tag="PKL26_03730"
     CDS             27..740
                     /locus_tag="PKL26_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNS82839.1"
                     /translation="MDPLIARIRKELSEHADPQIQKNSQRFFKEEIRCYGMKTARVVA
                     IAKQYWKEVKPRDKGEIFALCEDLYRSGYMEESFIVSEWAQRLSDNYEREDLSVFRHW
                     IGTYITNWASCDGFCNHTMGAFIEQYPDTIDELKRWTRSENRWMRRAAAVSLIIPAKH
                     GKFLEESLAIADLLLTDEDDLVQKGYGWLLKEASRMHTGEVFSFVMEKKRNMPRTALR
                     YAIELMPKELKAEAMKKDW"
     gene            826..1674
                     /locus_tag="PKL26_03735"
     CDS             826..1674
                     /locus_tag="PKL26_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS82840.1"
                     /translation="MTGTGRPVVLVHGWKSHPGIWDRLVEALDKDAIPSWTFDHSRVR
                     GAAVESLAALLQEFIRDQRADTGYCGPVDMVCHSMGTGIARYLIEVLDKNDRCEKVRL
                     LVGLGPPNNGSCLAELFFDPVHSPRILKTLGGIFVPRRFNPADDIIVQQFRPGSEAMA
                     ALRSAGIREDIRYRFILTGNCDADPEFFPPFHGKTWVMTANGWETTYAGDGIVPHADS
                     WLPGAEYDILSLDPSSLSRSPDLYCHLHLPRNPEVIGKILDSLSDPDTCAGTGCPECL
                     AMYRHL"
     gene            1761..>3425
                     /locus_tag="PKL26_03740"
     CDS             1761..>3425
                     /locus_tag="PKL26_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNS82841.1"
                     /translation="MYGWAGATLRIDLTTSSIERRALDAGRARKFLGGRGFNSRVLWE
                     EVAPGTDPLGPGNVLCYAPGMLTGTPLALTGRVSVSTLSPLGGILGDGSGGGEFAWQL
                     KRAGYDQIVVKGRSPYPCVLSIDDERVKICDARSLWGLDTWEATDRLRADLGDDTSVA
                     CIGPAGERLVRFASTIIDKYASAAIGSGAVAGSKNLKAIAVRGSNPPRLADPEGFRDL
                     AKIDREIFRNDPFFRNTVSKYGSIIGVEDWGPGFRNSQRRWTGADVPPALRKDAWKEY
                     EVGHTGCHGCPLRCKNIIRIPEGPRKGEVGEGLEYEAIHWLGINSGILDPIAIMEMSN
                     YADRQGMDVIGLGSVVGFAKELFEKGIISLEDTGGISLDWEDSESQRLLITMTAERKG
                     FGDLLALGMAGLAERLGKDAARYCFDVKGVTRGVYPSGIYSLAHATSTRGADHLRGRT
                     WAGGENEEGLYRDLVQSGYIPNDPAGALIVGERATTLADALGRCKGAVNSWNSSVPLV
                     RKYPLWGGAIRLIRAATGIEYTPRELEDALDRIYLLEMSFNVRQGKS"
BASE COUNT          725 a          955 c         1085 g          660 t
ORIGIN      
        1 tgccagggtc tcgcccacgt ttcatcatgg acccacttat cgctcgtatc cgaaaagaac
       61 tgtccgaaca tgcggacccc caaatccaga agaactccca gaggttcttc aaggaggaga
      121 tacggtgtta cgggatgaag actgccaggg tggtcgcaat tgcgaagcaa tactggaagg
      181 aggtcaaacc aagggataaa ggggaaattt ttgcactctg cgaggatctt taccgatcgg
      241 gctacatgga agagtcgttc attgtatctg aatgggcgca gcgcctctct gacaactacg
      301 aaagggagga tctttccgta ttcaggcact ggatcgggac gtacatcacc aactgggcat
      361 cctgcgacgg gttctgcaac cacacgatgg gggctttcat cgaacagtat cccgacacca
      421 tcgacgaact gaagcgctgg acccggtcag aaaaccgctg gatgcgcagg gcggcagcag
      481 tctcgctgat cattcccgca aagcacggga agttccttga agagtcgctc gcgattgccg
      541 acctcctgct cactgacgag gatgacctgg tgcagaaagg ctatggctgg ctcctgaaag
      601 aggcgagcag gatgcatacg ggggaggtct tctccttcgt catggaaaaa aagaggaaca
      661 tgcccaggac agcgctccgc tatgcaatcg agctgatgcc aaaggagttg aaagcggagg
      721 cgatgaagaa ggactggtag cggatggggc aatcggcccg cgttcgggca ggccgcctgc
      781 ccgattcaca acctctatcg gtgaagcaga cgacatattc tgttcgtgac gggaaccggc
      841 aggcctgttg tcctggtcca tgggtggaag agccaccccg gtatctggga ccggctcgtg
      901 gaagcactgg ataaggacgc aatcccttcc tggacgttcg atcactccag ggttcgcgga
      961 gctgcagtcg aatcgcttgc cgctctcctg caggagttca ttcgagacca gagggctgat
     1021 acggggtact gcggaccggt cgacatggtc tgccactcca tggggacagg gatcgcccgg
     1081 tacctcattg aagtccttga taaaaacgat aggtgtgaga aggtgcggct cctcgttgga
     1141 ctcgggccac ccaacaacgg gtcttgcctc gcggagctct tctttgatcc agttcactcg
     1201 ccgcgcattc tgaagaccct tggcgggatc ttcgtgccga gaaggttcaa tcctgctgac
     1261 gacatcatcg tccagcagtt caggcccggg agcgaagcga tggcggcact ccgttctgcg
     1321 gggatccgcg aagatatcag ataccggttc atcctcaccg ggaactgcga cgctgacccg
     1381 gagttcttcc ctcctttcca tgggaagacc tgggtcatga ccgcgaatgg ctgggagaca
     1441 acctatgcgg gagatgggat agtcccccac gcagactcct ggctcccggg ggcagaatac
     1501 gacatcctct ccctcgatcc ctcctcgctc tcccgctccc ccgatctcta ttgccacctg
     1561 cacctgccga gaaacccgga agtgatcggg aagatactcg actccctctc tgatcccgat
     1621 acctgcgcgg ggaccggctg cccagaatgc ctggccatgt acaggcacct ttaacaagag
     1681 aacgctgggg atctggagtc tcagcgggct ccttcgtctt gatggtgtga tttatcctct
     1741 tcgaagagag atgattctcc atgtacggct gggcaggggc cacactccgg atagatctca
     1801 ccacctcctc aatcgagcgg cgcgcccttg atgctggcag ggcgcggaaa ttcctgggcg
     1861 gaaggggttt taattcgaga gtgctctggg aggaggtagc tcccgggaca gatcccctcg
     1921 gcccagggaa cgtcctctgt tacgcaccgg ggatgctcac cgggacccct cttgcgctga
     1981 cagggagggt ctcggtcagc acgctctcgc cgcttggcgg gatacttggc gacgggagcg
     2041 ggggcgggga atttgcctgg cagctcaagc gggcgggcta cgatcagatc gtggtgaagg
     2101 gccgatcccc ttacccctgc gtcctctcca tcgacgacga aagggtgaag atttgtgatg
     2161 cccggtcact ctggggactg gatacgtggg aggcgacgga ccgtctccgg gcagatctcg
     2221 gcgatgatac gagcgtcgcc tgcattggcc cggcgggaga gcggctggtc cgctttgctt
     2281 cgacgatcat cgacaagtat gcctcggcgg caatcgggag cggggcggtc gccggttcaa
     2341 agaacctgaa agccatcgca gtgaggggtt cgaaccctcc cagacttgct gatcccgaag
     2401 ggttccgtga cctcgcaaag attgatcggg aaatattcag aaacgacccg ttcttccgca
     2461 acactgtcag caaatatggt tccatcatcg gggtagagga ctgggggccg ggctttcgga
     2521 acagccagag gcgatggacg ggtgcggacg tcccaccggc gctgcggaag gatgcatgga
     2581 aggagtatga agtcggccat accggctgcc atggctgccc gctccggtgc aagaacatca
     2641 tccgtatccc ggaaggtccc cggaagggcg aggtagggga agggctcgag tacgaggcca
     2701 tccactggct ggggatcaac agcgggatac tggaccccat tgccatcatg gagatgtcga
     2761 actacgccga ccggcagggc atggacgtga tcggccttgg gagcgtggtt ggttttgcaa
     2821 aggaactctt tgagaagggc atcatttccc tggaggatac cggtgggatt tcccttgact
     2881 gggaggattc agagtcgcag agacttctca tcaccatgac cgcggagcgg aagggttttg
     2941 gggaccttct cgcgctcggc atggccggac ttgcagagag actcggaaaa gacgcagccc
     3001 ggtattgctt tgacgtcaag ggggtaaccc ggggcgtgta tccctccggg atttacagcc
     3061 ttgcccatgc aacctcgacc aggggagcgg atcatctcag gggcaggacc tgggcaggcg
     3121 gcgagaacga ggaggggctc taccgggacc tcgtgcaatc aggatatatt cccaacgatc
     3181 cggcaggggc gctcattgtg ggagagcgtg ccacgacgct tgccgatgca ctgggcaggt
     3241 gcaagggggc ggtgaacagc tggaactcct cggttccgct ggtcaggaag tatcctctct
     3301 ggggaggggc gatccgcctc atccgggccg caacaggaat tgagtacacc cccagggaac
     3361 tggaggacgc cctcgaccgc atctacctcc ttgagatgtc attcaacgta cggcagggga
     3421 agagc
//
LOCUS       DAOQVJ010000129         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_32106_length_3384_cov_3.164314, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000129 DAOQVJ010000000
VERSION     DAOQVJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32106_length_3384_cov_3.164314"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKL26_03745"
     CDS             <1..345
                     /locus_tag="PKL26_03745"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="AroE, AroK; catalyzes the conversion of shikimate
                     to 3-dehydroshikimate and catalyzes the formation of
                     shikimate 3-phosphate from shikimate in aromatic amino
                     acid biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/shikimate
                     dehydrogenase"
                     /protein_id="HNS82842.1"
                     /translation="RTVERAEALAERFGCYHGSLRDFGSIRPDVVVNATPVGMESDTA
                     SPLKKSDLAPGMTVFDLVYTPPETPLIRIARSAGCTAITGKEMFIRQARAQFRQFTGI
                     DAPLEMIREFLG"
     gene            342..1346
                     /locus_tag="PKL26_03750"
     CDS             342..1346
                     /locus_tag="PKL26_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNS82843.1"
                     /translation="MNTFGRSFRITTFGESHGPALGVVIDGCPPGIALSEGDIQPFLD
                     RRRPGRSPHSSPRREEDRVEILSGVFEGRTTGAPIALLFRNSDSRPGDYGSLREVFRP
                     GHADYTWWRKYGIRDHRGGGRSSGRETVARVAAGAVAAKVLEQKGIRIGSRVLEIHGR
                     TSPEEMEAAIADAIQAGDSVGGVVEVTAGGCPPGLGDPVFGKLDAMIGLAMLSIGAVK
                     GVEIGDGFAAARVRGSEQNDQMEREGFLTNHAGGILGGISTGAEIVVRIAVKPTPSIA
                     KEQRTVDNAGEERVISVKGRHDPCIVPRLVPVAEAMLALVLADALMEQEKIGTWVTGG
                     "
     gene            1452..1868
                     /locus_tag="PKL26_03755"
     CDS             1452..1868
                     /locus_tag="PKL26_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82844.1"
                     /translation="MRETRWRYSLLLALMALATLLSGGCLSVLSQSGPDSVPAAAPGS
                     GLRAALVDQEADWGFSRGCTWTAELQVANPGNTSEKNVHLQVELVNARTGAVRDAKDL
                     FIGAIGPGEERTVRVVLDGECLDEYTVRAIPVVSGP"
     gene            complement(1855..2490)
                     /locus_tag="PKL26_03760"
     CDS             complement(1855..2490)
                     /locus_tag="PKL26_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNS82845.1"
                     /translation="MRCAVSVPDLAHLAFALSAGTDIIEIRLDLTGNIPEQTAHEVFS
                     ELPVPLIFTLRSTAEGGSFAGSPGKWWENLRPFLPYATYIDVEERFAQFAPRIREMGK
                     TVISSLHTGTMPDPHALVSLEARLRSFGDIPKIVVAPRTEEDVLALLSFTHRAERPII
                     TSIMGTGFRYARLLLPLFGSYLVFCHCGTAASPGQYHIREWREFCRLVRDH"
     gene            2502..>3384
                     /locus_tag="PKL26_03765"
     CDS             2502..>3384
                     /locus_tag="PKL26_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNS82846.1"
                     /translation="MPGRVFNLPTGDLSLLRMPSRSSPHTSVRCPELLAPAGSKEALH
                     AAIAAGADAVYLGGRKFGARHFAANFSEDEIREAVAHAHLRGVLIYVTVNTLVQDREL
                     PDVLAYLLSLYSMGVDAVLIQDGGLLALAREMLPGLCLHASTQCTISTSEGVAWAGKA
                     GFSRVVLARETPLTEIDRIMAIPPGERPGIEVFVHGALCYSYSGQCLLSSAIGGRSGN
                     RGMCAQPCRKPYCLVRGVPDRYGRLAGAEEVACPDRFLLSTRDLCCYPGLREIVRRGV
                     DALKIEGRMRSAEYVAAV"
BASE COUNT          617 a          996 c         1169 g          602 t
ORIGIN      
        1 cggactgtcg agcgtgccga ggcgttggca gagcgcttcg gctgctacca tgggtccctc
       61 cgtgactttg gcagcattcg gcccgacgtg gtggtgaacg cgacgcctgt ggggatggag
      121 tccgacacgg caagcccgct gaaaaagtcg gacctcgctc ctgggatgac ggtctttgac
      181 ctcgtctaca cgccccccga gacccccctg atcaggatcg cccggagcgc cgggtgcacg
      241 gccatcaccg ggaaggagat gttcatacgc caggcaaggg cacagttccg ccagttcacc
      301 ggtatcgatg cgccgctcga aatgatcagg gagttcctgg gatgaacacc tttggccgtt
      361 ccttcaggat tacgacattc ggggagagcc atgggcctgc actcggcgtc gtgatagacg
      421 ggtgtccgcc ggggattgcc ctctcggagg gggacatcca gccgttcctg gaccggagga
      481 ggcctggcag gagcccgcat tcttctcccc gccgggagga ggaccgggtg gagatcctct
      541 cgggagtctt cgaggggagg actaccgggg caccgatcgc gctccttttc aggaactccg
      601 actcacggcc cggggactat gggtcgctca gggaggtgtt caggcccggg catgcggatt
      661 atacgtggtg gaggaaatac ggcatcaggg accaccgggg tggcgggagg agctctggca
      721 gggagacggt cgcccgcgtt gcggcgggtg cggtcgcggc aaaggtcctg gaacagaagg
      781 ggatccgaat cgggagcagg gtcctcgaga tccacggtcg cacttcccct gaggagatgg
      841 aggccgcgat cgcggatgcc atccaggcag gggactcggt agggggagtc gtggaggtca
      901 ccgctggcgg gtgcccgcct gggctcgggg acccggtgtt cggcaagctg gatgccatga
      961 tcggcttggc gatgctctcc atcggcgcgg tcaagggcgt cgagattggg gacggctttg
     1021 ccgcggcgag ggtgcggggc agcgaacaga acgaccagat ggaaagagag ggatttctca
     1081 ccaatcatgc ggggggcatc ctcggcggca tcagcacggg tgcggagatc gtcgtgcgga
     1141 tagcggtaaa gccgactccc tccatcgcaa aggagcagcg aacggtggat aatgcagggg
     1201 aagagcgagt gatctcggta aaaggaaggc acgatccctg catcgtaccc cgccttgtcc
     1261 ctgtcgccga ggcgatgctc gcgcttgtcc ttgccgatgc cctgatggag caggagaaga
     1321 tagggacgtg ggtgaccggg ggatgaaagc atttatcctg cccggtcaac ccttcctgca
     1381 gttatggtgc gatgcacgca agtctttcat ccattccaga tacgacgctt gaataagggg
     1441 ggccgaaccc ggtgagagag acgaggtgga gatattcgct tctccttgcc ctcatggcat
     1501 tggcgaccct cctttccggt ggatgtctct cggtcctctc acagtcaggg ccggatagcg
     1561 tcccggcagc cgcccctggt tccgggctcc gggccgccct cgttgaccag gaggccgact
     1621 ggggattctc gaggggttgt acctggactg ccgagttaca ggtcgcaaat cccgggaaca
     1681 cgagcgagaa gaacgtgcac ctccaggtgg agctcgtgaa cgcgaggaca ggagcggtga
     1741 gggatgcaaa agacctcttt atcggggcga tcggtcccgg cgaagaaagg acggtccgcg
     1801 ttgtgctgga cggcgagtgc ctcgacgagt ataccgtgag ggcgatcccg gtcgtcagtg
     1861 gtccctgacc agccggcaga actccctcca ctcgcggatg tggtactggc cgggcgatgc
     1921 agcggtcccg cagtggcaga ataccaggta cgacccgaac agcgggagca ggagccgggc
     1981 ataccggaac ccggtcccca tgatactggt gatgatgggt ctttcggccc ggtgggtaaa
     2041 ggagaggagt gccaggacgt cctcctccgt acgcggggcg acgacgatct tcgggatgtc
     2101 cccgaaggag cgcagtctcg cctccagcga caccagcgcg tggggatcag gcatagtgcc
     2161 ggtatggagc gaggagatca cggtctttcc catctccctg atgcgcgggg cgaactgcgc
     2221 gaaccgctcc tccacgtcga tgtaggttgc atagggaagg aacggacgga gattctccca
     2281 ccacttcccg gggctgccag caaaggatcc cccctcggcg gtgctccgga gcgtgaagat
     2341 gagcggtacg gggagttccg aaaagacttc atgtgcggtc tgttccggta tatttccggt
     2401 gagatccagc ctgatttcaa tgatatccgt tccggcggac agggcgaacg cgagatgggc
     2461 aaggtccggg accgatacgg cacaccgcat tactccactt tatgcccggg cgagtcttta
     2521 acctgcccac gggcgacctc tcattgttga gaatgccttc gcgttcatcc cctcatacct
     2581 ctgtccgctg ccccgaactc ctggcacccg ccggatcgaa ggaagcactt cacgcggcga
     2641 tagccgccgg agcggatgcg gtgtacctgg gggggaggaa gttcggggca cggcacttcg
     2701 cggccaactt cagcgaggac gagatacgcg aggcggtcgc gcatgcacac ctccgcggcg
     2761 tcctcatcta tgtcacggtc aacaccctcg tccaggacag ggaactcccc gatgtccttg
     2821 cctacctcct ttcgctgtat tcgatggggg tcgatgcagt gctcatacaa gacggggggc
     2881 tccttgcact cgcccgcgag atgctgcccg gtctctgcct gcacgcatcg acccagtgca
     2941 ccatctccac gagtgagggg gtggcatggg ccgggaaggc aggattctcc cgcgtagtgc
     3001 tggcgcgcga gacaccgctc accgagatag accggatcat ggcgattccc cccggagaga
     3061 gaccggggat cgaggtcttc gtccatggag cactctgcta ctcgtattcc gggcagtgcc
     3121 tcctctcctc tgcgatcggg ggaaggagcg ggaacagggg catgtgcgca cagccctgcc
     3181 ggaagccgta ctgcctggtg cgaggcgttc ccgatcggta tggacggctc gcgggtgcgg
     3241 aggaggtcgc ctgccccgac cgcttcctcc tctccacgcg ggacctctgc tgctacccgg
     3301 ggctccgcga gatcgtcagg aggggggtcg atgccctgaa gatagagggc cggatgcgtt
     3361 ccgcggagta cgtcgcggca gtgg
//
LOCUS       DAOQVJ010000130         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_32614_length_3345_cov_4.640729, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000130 DAOQVJ010000000
VERSION     DAOQVJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32614_length_3345_cov_4.640729"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PKL26_03770"
     CDS             <1..1182
                     /locus_tag="PKL26_03770"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNS82847.1"
                     /translation="EPISSIDGILPHVPSFISRGVVVGHRDYRQIAEAIRDKRPFHVL
                     TGFMPSGHPHLGHLMVMKEMVWHVQQGGTGYITIADREAHAVRDISWQKCDEYGKEYL
                     QCLHALGYQGHTYFQSRNKRLMDLAFEAATKVNFSELQAIYGFSDETALAHAESVITQ
                     VADILYPQVDSPPAPTIVPVGIDQDPHIRLTRGVAHKLRMFTVEERDGYISVRSKNAP
                     PAALGEVHRRFAGSKKYEGHVDIPGGRCTDVSVEVRNIEIAHGGYGFFTPSSTYHRFI
                     PGLQGGKMSSSVPESTITFTEPDEVVRKKVMAALTGGRPTLAEQKEQGGEPDRCPLYL
                     LNLFHMVDDDAELAEIRRRCLAGELMCGQCKKETAERVLAFVRDFRERMEAVAHLVKV
                     E"
     gene            1184..2632
                     /locus_tag="PKL26_03775"
     CDS             1184..2632
                     /locus_tag="PKL26_03775"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNS82848.1"
                     /translation="MDLSLNEKRLLKVLEPLKRADPATLAARMETTAESVIQFAFLLE
                     GRGLARVEKKVDTTYHLTGEGARYARDGLPERQLLESFGERVPMSELTTHPLSRVGIG
                     QMRKKGWVKVCDGVVEKTGRDAPGEDEVALSDLSRGGDGVAILQKRGLLTEHQETRYL
                     VSLTPEGEALAAKGLDLEEEVGTLSREQIKSGAWKDLRIRRYSLETLPRKVFPGKVHP
                     YQRILDEMRQILLEMGFSEIYGGLAQSSFWNFDALFQPQDHPAREMQDTFFLKETVSL
                     PAGFERVRDMHLHGGTTSSTGWGGQWSREKAEQCVLRTHTTSLSIQHLVAHPEPPVKV
                     FCIGRVYRRESIDATHLAEFEQLEGIVMDRDVTFRHLLGFLKEFYGRMGFEDVRFRPG
                     YFPYTEPSVEPEVYVDGLGWVEMGGAGIFREEVRAPWGVTHPVLAWGLGVSRVAMLRL
                     GLKDLRQLYRTDIDFVRETPLWHQPGKTRGGA"
     gene            2634..>3345
                     /gene="pheT"
                     /locus_tag="PKL26_03780"
     CDS             2634..>3345
                     /gene="pheT"
                     /locus_tag="PKL26_03780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNS82849.1"
                     /translation="MAVITLYYRYLERLVGSDRETILARLPMIGSDIERVEEDHVDVE
                     FFPSRPDLFSVEGVARAMRGFLGLETGLPQYHVTPSGISFSVDPGLASIRPYLGTAVI
                     RGIHLDEEAILSLMGLQEALHWALGRGRSKVAIGIHDLDRVTPPFRYIAEERTRAFVP
                     LDYEREMTMEEILDEHPKGRAYADIVRGFPKFPLIVDACNQVLSFPPIINGELTRVTG
                     ESRNLLLDTPGTDERAVMT"
BASE COUNT          672 a          990 c         1089 g          594 t
ORIGIN      
        1 gaacctatca gcagcatcga tggcatcctc ccccatgtac cgtcattcat cagcaggggt
       61 gtggtggtgg gccaccggga ttatcgccag atcgccgagg ccatcaggga caaacggccc
      121 tttcatgtcc tgaccggatt catgccttcc ggccacccgc atctcggcca cctgatggtg
      181 atgaaggaga tggtatggca cgtgcagcag ggagggacag ggtacattac catcgcagac
      241 cgggaagccc acgcggtgcg cgatatctcc tggcagaagt gcgacgagta cgggaaggag
      301 tacctccagt gtctccatgc gctgggttac caggggcaca cctatttcca gagccgcaac
      361 aagcgcctga tggacctcgc cttcgaggcc gcaacgaagg tgaacttctc tgaactccag
      421 gcaatctacg ggttctcgga cgagaccgca cttgcgcatg ccgagagcgt gatcacccag
      481 gttgccgata tcctctatcc ccaggtggac agccccccgg cccccaccat cgtcccggtc
      541 ggcatcgacc aggacccgca catccgcctg accagggggg tcgcccacaa gctcaggatg
      601 ttcaccgtag aagagaggga cggatacatc agtgttcggt caaagaatgc ccctcccgca
      661 gccctcgggg aggtgcaccg gagatttgcc ggatctaaaa agtatgaagg tcacgtcgat
      721 atccccggag ggagatgcac cgatgtgagc gtcgaggtgc gtaatatcga gatcgcccat
      781 ggcgggtatg gctttttcac tccctcgtcg acctaccatc gcttcatccc cggcctccag
      841 ggcggaaaga tgtcgagcag tgtaccggag agcacgatca ccttcacgga gccggacgag
      901 gtggtgagaa agaaggtgat ggctgccctc acgggaggcc ggccgacgct cgcggagcag
      961 aaagagcagg gcggtgagcc cgacaggtgt cccctctacc tcctgaacct cttccacatg
     1021 gtggatgacg atgccgagct tgccgagatc cggaggcgct gcctcgcggg cgagctgatg
     1081 tgcgggcagt gcaagaagga gaccgcagaa cgggtgctgg cctttgtccg ggacttccgg
     1141 gagaggatgg aggctgtggc acacctggtg aaggtggagt gagatggacc tgtcactgaa
     1201 tgaaaaacga ctcttgaaag tccttgaacc gctgaaaaga gcagatccgg ctaccctggc
     1261 ggcccgcatg gagacgaccg ccgagtcggt gatccagttt gcctttctgc tggagggccg
     1321 cggccttgcc agggtggaga agaaggtcga caccacttac cacctcaccg gggagggagc
     1381 ccggtatgcg agggatggcc tgcccgagcg ccagctgctt gaaagtttcg gggagcgggt
     1441 cccgatgtcc gaactcacca cgcatccatt atcccgtgtc gggataggcc agatgcgaaa
     1501 aaaggggtgg gtgaaggtct gcgacggggt ggtggagaag accggccggg acgcgcccgg
     1561 ggaggacgag gtcgccctct ctgacctatc caggggtggc gacggggtcg cgatcctgca
     1621 gaagcggggc cttctcaccg agcaccagga gacacgctac ctggtatccc tgacccccga
     1681 gggcgaggcc ctcgcagcga aggggcttga cctcgaggag gaggtgggaa ctctctctcg
     1741 cgagcagatc aagagcggcg cctggaagga tcttcgcatc aggcgataca gccttgagac
     1801 cctgccccgg aaggtcttcc ccgggaaagt ccatccttac cagaggatcc tggacgagat
     1861 gcgccagatc ctcctcgaga tgggtttctc cgagatctac gggggcctcg cccagagttc
     1921 gttctggaac ttcgatgccc tcttccagcc acaggatcac ccggccaggg agatgcagga
     1981 cacgttcttc ctgaaggaga ccgtttcgct cccggccggg ttcgagaggg tgagagacat
     2041 gcacctccat ggagggacca cctcctcgac agggtgggga ggacagtgga gcagggagaa
     2101 ggcagaacag tgcgtgctgc gcacccacac gacgagcctc tccattcagc accttgttgc
     2161 gcatcccgag cccccggtaa aggtcttctg catcggcagg gtctaccgga gagagtcaat
     2221 cgacgccacc cacctggccg aattcgagca gcttgaagga atcgtcatgg accgggacgt
     2281 cacgttccgc cacctcctcg ggttcctgaa ggagttctac ggtcgcatgg gattcgagga
     2341 cgtccggttc aggcccggat acttccccta taccgagccg tcggtggaac ccgaggtgta
     2401 tgtcgacggc ctgggctggg tcgagatggg gggtgcaggg atcttcaggg aagaggtgag
     2461 ggcaccgtgg ggcgtcaccc atcccgtgct cgcatggggc cttggcgtaa gccgggtcgc
     2521 aatgctccgg ctgggattga aggatctccg gcagctgtac aggaccgata tcgactttgt
     2581 gcgggagacg cccctgtggc accagcccgg aaagaccagg ggaggtgcct gatatggcag
     2641 tcatcacgct gtattaccgt tacctcgaga ggctggtggg atccgacagg gagacgatac
     2701 tcgcgcgcct tcccatgata gggtctgata tcgagcgggt cgaggaggat catgtcgatg
     2761 tggagttctt cccctcgcgc cccgacctct tctcggtcga gggtgttgcg agggcgatgc
     2821 gagggttcct cgggcttgag acgggacttc cccagtacca tgtcacgccc tccgggatca
     2881 gtttctcggt cgacccgggg cttgcctcga tacgcccgta tctcggcacc gcggtcatca
     2941 ggggaataca cctcgacgag gaggcgatcc tctcgctgat gggcctccag gaggccctcc
     3001 actgggcgct gggccgcggc aggagcaagg tggcgatagg gatccacgac cttgaccgcg
     3061 tgaccccgcc attccggtac atcgcggagg aacgtaccag ggcattcgtg cctctggact
     3121 acgagagaga gatgaccatg gaggagatcc tcgatgagca ccccaagggg cgcgcctacg
     3181 ccgatatcgt caggggcttt ccgaagttcc ccctgatcgt ggatgcctgc aaccaggtcc
     3241 tctcgtttcc tcccattatc aacggcgagc tgacccgcgt gaccggtgaa tcgagaaacc
     3301 ttctccttga tacgccggga acagacgaac gggcggtaat gaccg
//
LOCUS       DAOQVJ010000131         3330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_32802_length_3330_cov_3.266260, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000131 DAOQVJ010000000
VERSION     DAOQVJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32802_length_3330_cov_3.266260"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKL26_03785"
     CDS             complement(<1..253)
                     /locus_tag="PKL26_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HNS82850.1"
                     /translation="MASYWFGDVDGGVCAPCPSHEPGFLSDRLDRIIQTRERHTPFSW
                     EAARECGFVRSRGEYVALLRSLALLRVEKELRRVSQLPEM"
     gene            complement(319..1530)
                     /locus_tag="PKL26_03790"
     CDS             complement(319..1530)
                     /locus_tag="PKL26_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNS82851.1"
                     /translation="MIEILAGTAAGVALGLLSGLLPGIHVNTLAAIMLASLAVLLPLF
                     GPFFLAAALFSALVTHCFIDAVPSTFLGIPDADTALAVLPAHALCMEGRGEEAVRISA
                     LGSATGLALAVPLTCILLLVIPHFQSVIDWASGIILVAVIGFLLVQSEAPGWGLLVFC
                     TSGLLGIFTFRYSFLAPPLLGESAVLMPLLSGLFGISMLLYSGKGAIPPQRFSGLDIS
                     REGILKGTVLGGAAGLVVGWLPGLSNATANGVLASAVNYTRDRRGYLLATSAANTMNA
                     VVGLAAFFAVGRMRNGVVAALALLEIPPLSFILFSGALAALAAYLLTIRLAGMAHLLE
                     GVDRTWLNRVVMAFVVVVCFLGAGLFGLFLLVTATAVGCIPRLVNVPQVFCMGAIMVP
                     VMFYSFGLGVI"
     gene            1613..2320
                     /locus_tag="PKL26_03795"
     CDS             1613..2320
                     /locus_tag="PKL26_03795"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNS82852.1"
                     /translation="MLIAITRLQEKSTDDQARCARFGHRCFPVSPLQARINQEEIAWF
                     VGETDRNTFDCIFFTSALPARLIGPRLSRWPRVVAIGPQTRRELEAIGIEAEVLPRYY
                     SRDFVDYLGPWIEGKHIGIPRADVPNPGLLSSIRDAGGKATEVACYSLIPTGERLSLG
                     DAEVLLFTSAMSYRKARWENRAGLILAAIGDITAGAMRDGGHHPSVVGDGSLEGTLEA
                     LNHYLDTHPRPERTGRR"
     gene            2317..3231
                     /locus_tag="PKL26_03800"
     CDS             2317..3231
                     /locus_tag="PKL26_03800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HNS82853.1"
                     /translation="MTPFPVPEHGLLVLDKPRGPSSHQVTAWAGEILGKRVGHAGTLD
                     PGVSGVLVVMVGNAVRLAPILLAEEKEYVAVIRTHGDVQEDALRSVASDFCGRIYQRP
                     PRRSAVARNLRIRTVRALEVREFHERLAVLGITCDAGTYIRSLCHHIGLALGCGAHMQ
                     ELRRVRSGIFSEEQAHTLHDLKDAATLAGQGDPQYLAGMILPPEKVARGLPTLVVRDT
                     AVDALCHGAALAGVGVVRQDAFARGEMVAVLTGKSELVCIGEAIVSSTQVPPGSTGLV
                     AAPRSVFMRPGTYPKGWKKREGAEKPEI"
BASE COUNT          724 a         1012 c         1065 g          529 t
ORIGIN      
        1 ccatctcggg cagttgggat accctgcgaa gttccttctc cacccgcagg agcgcaagcg
       61 agcgcagcaa cgcgacatac tctcctctcg atctgacgaa cccgcactcc cttgcagcct
      121 cccatgaaaa gggtgtatgt cgttcccttg tctggatgat ccggtcgagg cggtctgaaa
      181 gaaaacccgg ttcatgagag ggacatggtg cgcagacccc gccgtccacg tctccgaacc
      241 agtacgatgc catcagataa tctggtgccg ggaccggtat taatctcacc gggaaacatg
      301 cgccttcgat cggatacttc agatcacccc gagaccaaac gaatagaaca tgacggggac
      361 catgatggcc cccatgcaga agacctgcgg gacattgacc aggcggggaa tgcagccgac
      421 ggctgttgcc gttacaagca ggaacagccc gaagagaccc gcaccaagga agcagacaac
      481 gaccacgaag gccatcacca cccggttcag ccaggtacgg tcgactccct cgagaaggtg
      541 tgccatccct gccagccgga tggtgagaag gtaggctgcc agtgcggcaa gggcccctga
      601 gaaaagaata aaggagagtg gggggatctc cagcagcgcg agagctgcaa ccacgccgtt
      661 tctcatgcgg ccgaccgcga agaatgccgc gaggcccacc accgcgttca tggtgttggc
      721 ggcactggtg gcgaggaggt accccctccg gtcgcgggtg taattcactg ccgacgcgag
      781 caccccgttg gccgttgcat tggagagccc cgggagccat cccaccacga gtccggcagc
      841 accgccaagg acggtccctt tcaggatccc ctctctcgag atatcaagcc ccgagaaccg
      901 ctggggcggg atcgcccctt tccccgagta gaggagcatg gatatcccaa agaggcccga
      961 gaggagcggc atcagcactg cactctcccc gaggagcggg ggggcaagga atgaataccg
     1021 gaaagtgaat atgcccagga gccccgatgt gcagaagacc agcaggcccc acccgggagc
     1081 ttcgctctgc acgagaagaa agccgatcac cgcgaccagg ataatcccgc ttgcccagtc
     1141 aatgactgac tggaaatgcg gaatgacgag cagcaggata caggtaaggg gaactgcaag
     1201 tgcaagaccg gttgcgctcc ccagcgccga gatcctgacc gcctcctccc cccgcccctc
     1261 catgcagagg gcatgggcgg gcaggaccgc aagagccgtg tcggcatccg gtatgccaag
     1321 gaatgtgctt ggtaccgcgt cgatgaagca gtgggtgaca agcgccgaga aaagggccgc
     1381 cgcaaggaag aatggaccaa aaaggggaag gagaaccgcg agggacgcaa gcatgattgc
     1441 ggccagcgtg ttgacatgga tcccaggcag gagcccggag aggagcccga gtgccacgcc
     1501 tgccgcggtc cccgccagga tctcgatcat cctcgcctag ggtggtgggg aattaagata
     1561 aaggaatcga aacagataga acaccactct gagaagtgcc gggtgtctct tcatgctgat
     1621 cgccatcacc aggttacagg agaaaagcac cgacgaccag gcgcgatgcg ccaggttcgg
     1681 acaccggtgc ttccctgtct ccccgctgca ggccaggatc aaccaggagg agatcgcatg
     1741 gttcgtgggg gagacagacc ggaacacctt tgactgcatc ttcttcacga gcgcgcttcc
     1801 tgcccgcctg atcggcccgc gcctctcgcg ctggccgcgg gtggtggcaa tcgggcccca
     1861 gaccaggagg gaactcgagg cgatcggaat cgaggcggag gtgcttccca ggtactacag
     1921 cagggacttc gtggattacc ttggcccatg gatagaggga aaacacatag gcatcccccg
     1981 tgcggatgtc ccaaacccgg gactcctctc gtccatacgg gatgcagggg gaaaggcaac
     2041 agaggtcgca tgctactccc tgatccccac aggggaacgc ctttccctgg gggacgctga
     2101 agtgctcctc tttacgagcg ccatgtcata ccggaaggcc cgctgggaga accgcgcggg
     2161 actgatcctt gcggccatcg gcgatatcac ggccggggcc atgcgggatg gcgggcatca
     2221 cccctctgtc gtgggagacg gttcgcttga ggggacgctc gaggcgttga accattacct
     2281 ggacacccat ccacggccgg aacggactgg aaggagatga ccccgtttcc cgtccctgaa
     2341 cacggcctgc tggtgcttga caagcccagg ggcccttcaa gccaccaggt gactgcctgg
     2401 gcgggtgaga tcctcgggaa gcgggtcggt cacgcaggga cgctcgaccc cggggtctcc
     2461 ggggtgcttg tggtaatggt gggaaatgcc gtccgccttg cccccatcct cctcgcggag
     2521 gagaaggagt atgtcgcggt catccgcacg catggcgatg tgcaggaaga cgcattgaga
     2581 agcgtcgcct ctgatttttg cgggcgcatc taccagcggc cgccccgcag gagcgcggtc
     2641 gcccgcaacc tcaggatacg gacggtaagg gcgctcgaag tgcgtgagtt ccatgaacgg
     2701 ctggcagtac tcggcatcac ctgcgatgcc gggacctaca tacggtcact ctgccaccac
     2761 attggactgg cccttggctg cggcgcacat atgcaggaac tccggcgggt gagatcgggg
     2821 attttttctg aggaacaggc acacacgctt cacgacctga aagatgccgc aacgctcgcg
     2881 ggccagggag acccgcagta tctcgcaggg atgatcctcc ctccggagaa ggttgcccgc
     2941 gggcttccca cgcttgtcgt ccgcgacact gccgtggacg cactctgtca tggcgcagcg
     3001 ctcgccggag tcggggtcgt ccgccaggac gcattcgcga ggggagagat ggtggcggtg
     3061 ctcacgggga agagtgaact ggtctgtatc ggcgaggcga tcgtctcctc cacgcaggtg
     3121 ccgccgggca gcacgggtct cgtcgcggcg ccaaggagcg tattcatgcg cccgggcacg
     3181 tacccgaaag gctggaaaaa gagagaggga gcagaaaagc cggagatatg agccctggag
     3241 atcccccggc aggaagtctc ggccccgcgg agttcctgtc cctctttcca gaaggatgag
     3301 atagcattaa aaaaacgccc gatccatagt
//
LOCUS       DAOQVJ010000132         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_32849_length_3327_cov_4.191932, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000132 DAOQVJ010000000
VERSION     DAOQVJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32849_length_3327_cov_4.191932"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKL26_03805"
     CDS             complement(<1..414)
                     /locus_tag="PKL26_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNS82854.1"
                     /translation="MSRITPRKRHTIRRSQAQELLSSLREQIGDAAEMFSAERMEILE
                     TDAAISLYLVDRKPLLMRLQGMVFPTLRGLLEHPFPSRRVVVDSGAVPFVIKGADVMR
                     PGITSISDDVTAGMPVQVVEERHGKPIALGIALFDA"
     gene            612..842
                     /locus_tag="PKL26_03810"
     CDS             612..842
                     /locus_tag="PKL26_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNS82855.1"
                     /translation="MTKRPLDILDQVLNRQPVIVSLKGGRELRGVLQGYDVHMNLVLD
                     KAEEIENGQARSVGTLIVRGDNVIYISPSLES"
     gene            886..1068
                     /locus_tag="PKL26_03815"
     CDS             886..1068
                     /locus_tag="PKL26_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HNS82856.1"
                     /translation="MSKGTPSMGKRNHKTHIACRRCGRVSYHLRHKTCSACGFGKSTK
                     IRGYKWTMKRPKVPTH"
     gene            1078..2499
                     /gene="purF"
                     /locus_tag="PKL26_03820"
     CDS             1078..2499
                     /gene="purF"
                     /locus_tag="PKL26_03820"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNS82857.1"
                     /translation="MCGIVGIRDAGGVSFSIYYSLYALQHRGQESAGISTFDGTRLHK
                     HKGQGLVAEVFDSTILESLEGNVGIGHVRYPTTGANRPENAQPFNFVYRDHIISIAHN
                     GNLVNSRELRDEYECRGQIFCSTTDTELIAKVITEEMSNSGCIEDAVHRCMRVLKGSY
                     SVVMMIDGTLYGFRDPLGIKPMCIGKAGQALVLASESVAIDALAGTFLRDVAPGELIC
                     IDDQGMRSMQIAVSRRKAHCIFEYIYFARADAVLDGALVYDVRLKIGQKLFEEDPVKA
                     DSVCPVPDSGTAYAIGYSARSGTPFVESLLKNRYMGRTFIMATQKEREMAVRIKLNPI
                     REHLDDKSVVLVDDSIVRGTTSRRIIGIMRDAGAREIHVRIGSPAIKAPCYLGVDMPT
                     REELIASDKEEEEVRKRITATSLHHISIDALVEAIGIDRANLCTGCLTGCYPVDIPGE
                     KSSPPPVDFLDGTYQTRLGSFGS"
     gene            complement(2584..2658)
                     /locus_tag="PKL26_03825"
     tRNA            complement(2584..2658)
                     /locus_tag="PKL26_03825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2621..2623),aa:Met,seq:cat)
     gene            2782..3201
                     /gene="trxA"
                     /locus_tag="PKL26_03830"
     CDS             2782..3201
                     /gene="trxA"
                     /locus_tag="PKL26_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNS82858.1"
                     /translation="MEDEELQRIREKKRKELEERLHAPHEIQPSAKVLLVEEANFRSL
                     LSQHPRLVVDFWAEWCGPCRMVGPVIDALCQEMAGKVIFGKCNTDHNPLIAAQFGISA
                     IPTVLLFNGGQLADRIIGAYPKEAIKTRICRAYGLPT"
BASE COUNT          771 a          927 c          962 g          667 t
ORIGIN      
        1 tgcatcaaag agcgcgatgc cgagggcaat cggctttccg tgccgttcct cgaccacctg
       61 gacgggcatg cctgcagtca cgtcgtcaga gatcgaggtg atccccgggc gcatcacgtc
      121 cgcacccttg atcacgaacg gcaccgcgcc ggaatccacc accaccctgc gtgaagggaa
      181 cgggtgctcg aggagaccgc ggagcgtggg aaagaccatt ccctggagac gcatcagcaa
      241 gggtttcctg tctacgaggt acagcgagat agcggcatcc gtctcgagga tctccatccg
      301 ctcggcggag aacatctctg cggcatctcc tatctgctcc cggaggctgg agaggagttc
      361 ctgcgcctgc gatctccgga tggtatgccg ttttctcggg gtgattcgcg acattcagtt
      421 catattcata ttgctgcaca cacaaaaaaa acgcatccat cccagaaggg gagaaatttt
      481 cttctttatt gcccattaat gccctcaata ggtgaatgaa ggctgggaaa cgcctccgta
      541 taagacagta catttaagta tccgtggacc gcacatatat taccccaaag tgactagaag
      601 tgggtacgat tatgaccaaa cggccgttgg atattttgga tcaggtgctg aaccggcagc
      661 ctgtcatcgt atccctcaag ggcgggaggg aactccgcgg ggtcctgcag ggttatgatg
      721 tgcacatgaa ccttgtcctc gacaaggcag aggagataga gaacgggcag gcccgaagcg
      781 tcgggaccct catcgtgcgc ggggacaacg tgatttacat ctcgccgtcc ctcgagtcat
      841 aaacagtcta taagggaata tccatctcta aaaaaaaggt gaaccatgtc aaaaggaact
      901 ccgtcaatgg gaaagaggaa tcacaagacg cacatagcct gccgcaggtg cgggcgcgtc
      961 tcctaccacc tgcggcacaa gacctgctct gcctgtggat tcgggaagag cacgaaaatt
     1021 cgcgggtata agtggacgat gaagaggccg aaggtcccga cccattaaag gcctcctatg
     1081 tgcggaatcg ttggcatcag ggatgctggc ggtgtttcct tctctattta ctattcgctt
     1141 tatgctctcc agcacagggg acaggagagc gcagggatct caacgtttga cggcacaagg
     1201 ctccacaagc acaagggcca gggccttgta gccgaggtat tcgattctac catcctcgag
     1261 agccttgaag gaaacgtggg tatcggccac gtccgatacc cgaccaccgg tgcgaatcgc
     1321 ccggagaatg cacaaccgtt taattttgtc taccgcgacc atatcatctc gatcgcccat
     1381 aacggaaacc tggtcaacag ccgggaactc agggacgagt acgagtgccg gggccagatc
     1441 ttctgctcga ccaccgacac agaactgatt gcaaaggtca tcaccgagga gatgagcaac
     1501 tcggggtgca tcgaggacgc ggtgcaccgt tgcatgcggg tattgaaggg ctcctattcg
     1561 gtcgtgatga tgatcgacgg aaccctctat ggatttcggg acccgctcgg gataaagccc
     1621 atgtgcatag gaaaagcggg gcaggcgctg gtcctcgcat cggagagcgt cgccatcgac
     1681 gcccttgccg ggacattcct ccgcgacgtc gcccccgggg agctcatctg catcgatgac
     1741 cagggaatgc gcagcatgca gatcgcggtc tcacggagaa aggcccactg tatcttcgaa
     1801 tacatctatt ttgcccgtgc agacgcggtc ctcgacggcg cactcgtata cgacgtccgc
     1861 ctgaagatcg gccagaaact cttcgaggag gacccggtga aggcggattc tgtctgcccg
     1921 gtgcctgact cggggactgc atatgccatc gggtactcgg cccgttcggg cacgcccttt
     1981 gtcgagagtc tccttaagaa ccggtacatg ggccggacct tcatcatggc tacccagaag
     2041 gagcgcgaga tggcggtacg catcaagctg aaccccatcc gggagcacct ggacgacaag
     2101 tccgtggtgc tggtggacga cagcatcgtg cgggggacca catcccgtcg gattatcggg
     2161 atcatgagag atgccggtgc ccgcgagatc catgtgagga taggctcccc tgccatcaag
     2221 gccccctgtt accttggagt tgacatgccc acccgggagg aactgatcgc aagcgataag
     2281 gaggaggagg aggttcgaaa gaggatcacc gcgacgtctc tccatcatat ctcaatcgat
     2341 gccctggtgg aggcgatcgg catcgaccgg gcgaaccttt gcaccgggtg cctgaccggc
     2401 tgttaccctg tggatattcc cggagaaaag agcagcccac caccggtcga tttccttgac
     2461 gggacctacc agacacggct cgggtcgttc gggtcctgaa agtgcctctc ccgcaccatt
     2521 cctttttttt taaaaagaga gttactttgc ggtcgcaaga aagatgaacg ggaaaatcaa
     2581 aaatagcgag ggatggattt gaaccatcga tctacgggtt atgagcccgt cgggatatcc
     2641 tgactacccc acctcgcttc cgcggattcg cgcaacatag ttgacaatct gcggtgatat
     2701 agtttgcccc ttgagacttg ttccggcagg ccatggcggc ggagtgatta tctgttctcg
     2761 ctcccaacac gaagaataac catggaagac gaggaattgc agcgtatacg ggagaagaag
     2821 cggaaggagc tcgaagagag gcttcacgct ccgcacgaga tacagccctc cgcaaaggtc
     2881 ctcctcgtgg aggaggcgaa ttttcgatcg cttctctccc agcacccgcg gctcgtggtg
     2941 gacttctggg ccgagtggtg cggtccatgc aggatggtcg ggccggtgat agacgcgctc
     3001 tgccaggaga tggcgggaaa ggtcatattc gggaagtgca acacagacca taaccccctg
     3061 atcgcggccc agttcggcat ctctgccatt ccgacagtcc ttctcttcaa cggagggcag
     3121 cttgcggatc ggattattgg ggcgtatcct aaagaagcga taaaaacccg gatctgcagg
     3181 gcctacggcc tccccacgtg agggggtgca ccgtccggta aatgagggag ggcgactgcc
     3241 ctgcctcaag tgatcttttt tttaagaatc ccccatcaca accatattaa gttccgccgg
     3301 atcaccactg tacccgatgg aaaagac
//
LOCUS       DAOQVJ010000133         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_32866_length_3326_cov_4.168450, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000133 DAOQVJ010000000
VERSION     DAOQVJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32866_length_3326_cov_4.168450"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKL26_03835"
     CDS             complement(<1..328)
                     /locus_tag="PKL26_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS82859.1"
                     /translation="MNDLEEFELTLQEIVSRGGEEIAEAWMKDIEVEYGRAPLIFKRM
                     AERPEVLISHLLYKTAVIKTSAIDPKYTELISMAVGAALRCPHCTSYHMQAAVKKGAT
                     REEILEV"
     gene            660..2084
                     /locus_tag="PKL26_03840"
     CDS             660..2084
                     /locus_tag="PKL26_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNS82860.1"
                     /translation="MFAPVTYSRADIASAAAPWKRDHSSPGIVMRESGMGETLPIVSG
                     TQKFNFTIPERNTTTVPGRNISFNRPVGKASLVSGTGTVVYMDLEGGFYGIIADDGAR
                     YLPDLLPASCKVDGIRVKFTGMEKSGEASIYMWGTSLRILTAMPLGEEVKAEGRIQYI
                     DLEGGFYGIVAADGRSYLPLNLNKEFQVDGMKVSFTARTVPDTLGIYMWGIPVTVISI
                     SARQGEDKSASQSLIGPWTLSGMARGAAVAPVIQGTTITAEFTSDGRVSGTAGCNLYF
                     APFEQSGTTLRIGPAGSTEMYCFDASRTIMKQESTYLQLLGEAASWKISGGNLVISDA
                     SGQEILVFSPGIADASDRTEPLVEFWRTGGFAGMNDHLEVYPDGSVSLTRKEYSVGFT
                     LSEADLGTLNSLLQESGFMALSSEYRAAPGSADLFLYQVRAHGKTVLAEDTVIPGSLQ
                     PLIDELVRLVVENAPDDIAPPRIH"
     gene            2299..2802
                     /locus_tag="PKL26_03845"
     CDS             2299..2802
                     /locus_tag="PKL26_03845"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNS82861.1"
                     /translation="MLFVAMAVLLSFSGCISPQDFVAEPGPGPTGTLTPATTPTAGIP
                     AEEGGIGGLPVVRGGTSQYFVKMPYGYMVTSPTTGVRLSVIEIKDDLDATGQRVISGK
                     IKNDENYRIDHITVYFNLYNSNGNLIGNAYASVNSLGPGKVWRFTTNPFAYKDYQYYE
                     MAGTFTA"
     gene            complement(2845..>3326)
                     /locus_tag="PKL26_03850"
     CDS             complement(2845..>3326)
                     /locus_tag="PKL26_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-coenzyme M reductase family protein"
                     /protein_id="HNS82862.1"
                     /translation="RGINVPIVLCGGPPVEALRAIIDPPVDGYVGGVGRFMHRTKEAE
                     EISRLDDVVAEVTRVLDRKREEMAKDPLSISPARLMDIISEKIDDIHEVLSPTPITVQ
                     MTGLRVKLPYDAYASRLRATVVDEGVTIGDLAEVLPSRMRDYILVRIKPFSETNMMV"
BASE COUNT          704 a          944 c          932 g          746 t
ORIGIN      
        1 tcacttccaa aatctcctcc cgtgtcgctc ctttcttcac cgcagcctgc atgtggtagc
       61 tggtacagtg cgggcaccgc agagccgcac cgaccgccat gctgataagc tcggtatact
      121 tggggtcgat ggcactggtc tttatcaccg cggtcttgta gagtaggtgg gatatgagca
      181 cctcgggccg ttctgccatc cgtttgaaga tgaggggtgc acggccgtac tccacctcga
      241 tatccttcat ccatgcctct gcgatctctt ctccaccgcg ggatacgatc tcctgaaggg
      301 tgagttcgaa ctcctcaagg tcgttcatgt gcagccttcc agtctacata tcgtcctgca
      361 ggactcatta tactgcctgc aagggcccga taggtgtgaa aaaattcgca gtgaaagtca
      421 tgtttttatc cgagttgcgc caaagatctg gatggttccc agacaattga ggaccaggag
      481 agatatatat ggtaaactgg agtccttttc tacactccac atgcctggtg ctgctcgtcg
      541 cgctttgttt cggagcgata ggagcggtcc aggctgcacc gatgagatac gatcttaccg
      601 aaaaattttc atcgcaccgg atgcaggata acagtcctgg caagatgccg ggaggaaaca
      661 tgtttgcacc ggttacgtat tcccgcgctg atattgccag tgcagccgca ccctggaaga
      721 gagatcactc ttccccgggc attgtcatgc gcgaaagcgg catgggtgag acacttccga
      781 ttgtgtcggg cacccagaag ttcaacttca ccatccctga acggaacacg acgacggttc
      841 ctggcaggaa catctcattc aaccgcccgg tcgggaaagc atccctcgtg tcaggcaccg
      901 ggaccgtggt ctacatggat ctcgagggcg gcttctatgg catcatcgcc gatgacggtg
      961 cccgttacct ccccgatctc ctcccggcat cctgcaaggt ggatgggata agggtgaagt
     1021 tcacgggcat ggagaagagc ggggaagcca gcatctacat gtggggaaca tcccttcgca
     1081 tcctgacggc aatgcctctc ggtgaagagg taaaggccga ggggcgtatc cagtatatcg
     1141 accttgaggg cggcttctat ggcatcgtcg ccgctgatgg aaggtcttac ctccccctga
     1201 acctgaacaa agaattccag gttgacggga tgaaggtctc ttttactgcg agaacggtcc
     1261 ctgacaccct gggcatctac atgtggggaa ttccggtcac cgtcatctcc atatctgcaa
     1321 gacaggggga ggacaagtcg gcttcccagt ccctcatcgg gccatggacc ctttccggca
     1381 tggcaagggg agctgcggtc gcccctgtga tccaggggac tacgatcacg gcagagttta
     1441 ccagtgatgg cagggtcagc gggaccgcag gctgtaacct ctattttgca ccattcgagc
     1501 agagtggcac taccctccgc atcgggcctg caggttcgac agaaatgtac tgtttcgatg
     1561 cgtcgagaac gatcatgaag caggagagta cctacctgca gctcctgggg gaggcagcat
     1621 cctggaagat cagtggaggc aatctcgtga tctcggacgc atcaggccag gagatactgg
     1681 tcttttcacc cggtattgcg gatgcatccg acagaacaga acctctcgtt gagttctgga
     1741 ggaccggcgg gtttgcaggg atgaacgacc acctggaagt gtatcccgac gggagcgttt
     1801 ccctgacccg gaaggagtat tctgtcgggt tcacactctc tgaagcagac ctcgggaccc
     1861 tcaactcact tctccaggaa tcagggttca tggccctctc ctccgagtac cgggctgctc
     1921 ccgggagtgc ggatcttttc ctctaccagg tccgcgcaca tgggaagacc gtgctcgcag
     1981 aggacaccgt gataccggga tccctccagc cccttatcga cgagcttgtc cgcctggtgg
     2041 tggagaatgc cccggatgac atcgcacccc cacgaattca ctgaactcac ttccccctcc
     2101 ttttttctcg ttgtcctgtt gcccatagag aatacctgca tcccctagaa ggaccctcac
     2161 cggaaggacg acacctcttc ccctttttaa ggcagtcgca cgtgaacgga ccgaccgatt
     2221 ccttttatat cagcgcaacc gagagtcttg tgcatgagag gggtcggaat gaaggtatgg
     2281 cgcaatattc actggttgat gttatttgtg gcaatggcgg tgctcctctc gttttctggc
     2341 tgcatctcgc ctcaggactt cgtggcagaa cccggccccg gtcctacagg gacgctcacc
     2401 cctgcaacga ctcccaccgc cggcatcccc gcagaggagg gaggcattgg tggactgcca
     2461 gtggtccggg gagggacatc acagtatttc gtgaagatgc cgtacggata catggtgacg
     2521 agtcctacta ccggggtccg tctctcagtc atagagataa aagatgatct cgatgccact
     2581 gggcagcggg tcatcagcgg gaagatcaag aacgacgaga attatcgcat cgaccatatc
     2641 actgtctatt tcaacctgta caattcgaac ggaaatctca tcgggaatgc atatgcaagc
     2701 gtgaactcgc ttggccccgg aaaagtgtgg aggtttacga caaacccgtt cgcctataaa
     2761 gattaccagt attacgagat ggccgggaca ttcaccgcct gaacgagggg gattgagggg
     2821 ccctccctcc cacatgtcgc ccgttcagac catcatgttg gtctctgaaa atggctttat
     2881 cctgacgagg atgtagtctc tcattcggga gggtaaaacc tctgcgaggt ccccgatcgt
     2941 cactccttcg tcgaccactg ttgcccggag ccgtgacgca tacgcgtcgt atggcagctt
     3001 gacccggagc cccgtcatct gcacggtgat gggggtcggg gagaggactt cgtggatgtc
     3061 gtcaattttt tcggatatga tgtccatcag gcgtgcaggg gagatggaga gtgggtcctt
     3121 ggccatctcc tcacgtttcc tgtcaagcac gcgtgtgact tctgccacca cgtcgtcaag
     3181 ccttgatatc tcttcggctt ccttggtccg gtgcatgaaa cggccaaccc cgcccacgta
     3241 tccgtccacg ggtggatcga tgatggccct gagggcctct accggcggac cgccgcagag
     3301 gacaatgggg acattgatcc ccctgc
//
LOCUS       DAOQVJ010000134         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_32997_length_3317_cov_4.367259, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000134 DAOQVJ010000000
VERSION     DAOQVJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32997_length_3317_cov_4.367259"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKL26_03855"
     CDS             <1..409
                     /locus_tag="PKL26_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNS82863.1"
                     /translation="EISLAGAWMKAREIEFSTIASSPLRRAAETASIIARALGDPDRV
                     TFWDELAIGPPPGTVVQKIREIMPENRILLVGHEPQLSSLVSLLICSRHECNIVLKKG
                     GIARVRFHGDPGSGELLWLLSPGMIQEWHSKER"
     gene            complement(425..1147)
                     /locus_tag="PKL26_03860"
     CDS             complement(425..1147)
                     /locus_tag="PKL26_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS82864.1"
                     /translation="MASEQGLTSIDSQLKGKILEMLQEKDMDFEEIVASSGRAKSTIS
                     AHMKALSLAGITTTRPDPMDARKRIFSLNGRVLVRAHTDDTELLWADRFFPDALPGDA
                     TTRDVFRFILTSLRVSLLAEGISIYPILERAGRKAGAAIYPQVRAAELSVFLGNLSAF
                     WARFGLGEMEVEREDPLAIVIKDCFECMDLPITGRPECAFDTGLLATLFSAYYGEMHE
                     VVETHCYATGSNFCRFEVWNAR"
     gene            1414..1686
                     /locus_tag="PKL26_03865"
     CDS             1414..1686
                     /locus_tag="PKL26_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS82865.1"
                     /translation="MTLASTPAQVNGPDIRGPVNSIDCLPSSAQAVFKLLGDGKERTL
                     NEIIGRVAFSPRTIRNALNKLRQSGLIVAKFNFKDARKPLYKLKTG"
     gene            1813..2535
                     /locus_tag="PKL26_03870"
     CDS             1813..2535
                     /locus_tag="PKL26_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82866.1"
                     /translation="MKHQYLGDTRDLFKYDLIETLLSTLPSLTQAFFIPMLTPDDGSG
                     HGRRTGYFRTRAGNLNAGLADFLSKLRMRKSRDCFMVRDYFQEKSCEVSFYRARFTHR
                     GRGAYFSRIAKKVRGLPHTLVFLDPDTGLEVASPGAGHLLYSEVSLIARSLGPGSLLM
                     IYQHIPRTPRIAYIEHRCASLFEHTGILPSWISDGQVVFFFLCPEQNVGYELDHCLER
                     YRIRYPLLLARQEEGAGFIHRR"
     gene            complement(2519..>3317)
                     /locus_tag="PKL26_03875"
     CDS             complement(2519..>3317)
                     /locus_tag="PKL26_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNS82867.1"
                     /translation="RMLAKAVRDPHNASVLLRMADQELGHSRIWEHYTGKVAGPAVAR
                     VWYYYIIARLLGYTFAAKLMENRENSAIQDYGTLVGTIPEVEGIIAEEEQHEMEILGM
                     LEEDRLRYVGSIVLGLNDALVEFTGSLAGFTFALQDTTVIAAVGCIMGVAAALSMASS
                     EYLSQKADQTGREPAKAALYTGGAYIFTVFLLVVPFLLMGNPFSAIFVSLITAVAIIV
                     CFTFYTSVAGGLPFWRRFLEMVAISLGIAGVSFLIGLLIRGLFNVDV"
BASE COUNT          779 a          955 c          878 g          705 t
ORIGIN      
        1 ggaaatctcc ttggcaggcg cctggatgaa ggcacgggag atcgagttct caacgatcgc
       61 ttcgagcccg ctcaggcggg ccgcggagac tgcatcgatc atcgcaaggg cactgggaga
      121 tcctgatcgg gtcacattct gggacgaact ggccatcggt cccccgccgg gaacggtcgt
      181 acagaagatc agggagatca tgcccgagaa caggatcctg ctggtaggcc acgagcctca
      241 gctctcttca ctcgtctctc tcctcatctg cagccggcat gaatgcaata ttgtcttaaa
      301 aaaaggcggc atcgcgcgtg tacggtttca tggagaccca ggaagcggag aactcctctg
      361 gctgctttcc cccggcatga tccaggaatg gcattccaag gaaagataac ggatagaacg
      421 ccactcaacg ggcgttccag acctcaaacc tgcagaagtt gctccccgtg gcatagcagt
      481 gggtctcgac gacctcatgc atctcgccat aatatgcgga gaacagggtt gcaagaagcc
      541 ccgtatcgaa tgcacactcg ggcctccctg tgatcgggag gtccatgcac tcgaagcagt
      601 ctttgatcac gatagccagg gggtcctccc gctcaacctc catctccccc agtccgaaac
      661 gggcccagaa cgccgagaga ttcccgagga agacagagag ttccgcagcc ctgacctggg
      721 ggtaaatggc ggcgccggct ttccgcccgg ccctctcgag tatcgggtat atgctgatcc
      781 cttccgcaag aaggctcacg cgaagcgatg taaggatgaa acggaagaca tctctcgtgg
      841 ttgcatcgcc gggaagcgca tcaggaaaga atcgatcggc ccagagcagt tcggtatcat
      901 ccgtgtgagc ccggacgagg acccgcccgt tcagggagaa tattcgtttc ctcgcatcca
      961 tcggatcggg cctggtggtg gtgatacctg caagagagag ggctttcatg tgggcagata
     1021 ttgtcgattt cgcccttccg gaactggcga caatctcttc aaaatccatg tccttctcct
     1081 gaagcatctc gaggatcttt cccttgagct ggctgtcaat tgacgtgagg ccttgttcgg
     1141 aggccacaat ctgtatgtac ctcccgggac ggtcctgatt gtggggggga gtagtcatgg
     1201 cgtcagttct cacatctcag tgggggcaaa aatatatata agtatagttc gtatatagta
     1261 gaatgttcgt atacactcga acaaagtgtg tgccaccctg cccatgaccc gggcaaccgg
     1321 cacgcatccc gggattatat tcccgcgaaa tatgtgggaa gacatcccgg agagaacgaa
     1381 taacgaacct ctcatgaaca caggtaaaaa ttcatgacac tggcatcaac cccagcccag
     1441 gtgaatgggc cggacatcag ggggccagtc aacagcatcg actgcctccc ctcctctgca
     1501 caggcagtat tcaagctgct cggcgatgga aaagagcgga cgttgaacga gatcatcggc
     1561 agggtggcat tttcaccacg gaccatacgc aatgccttaa acaagctcag gcagagcgga
     1621 ctgattgtgg caaaattcaa tttcaaggat gcacgaaaac ccctctataa gttaaagacc
     1681 gggtgatccg gcggctgcag gaatatgcct gtccccccat ccgggtggga ccctgtatcc
     1741 gtcatctttt ctcctcatct tcatgaaaac accggaaggt ttctttcccg tcccgcaaga
     1801 gaactcttcc cgatgaagca ccagtacctg ggtgatacac gggacctctt caagtacgac
     1861 ctcatcgaga cgctgctttc cactctccca tccctcacac aggctttctt cattcccatg
     1921 ctcaccccgg atgacgggtc agggcacggc cggagaacag gatattttcg tacaagagcc
     1981 gggaatctga atgccggtct tgcagatttc ctctcgaagc ttcgcatgcg caaatcccgg
     2041 gactgcttca tggtacgcga ctattttcag gagaaatcgt gcgaggtcag cttttacagg
     2101 gcgcgtttca ctcaccgcgg cagaggggca tatttctcgc gcattgccaa aaaagtccgt
     2161 ggactcccgc acaccctcgt attcctggat cccgacactg gcctcgaggt cgccagtccg
     2221 ggtgccggac acctcctcta ttccgaagtc agtcttatcg cccgctccct cggaccgggt
     2281 tccctgctga tgatttacca gcacattcca aggacgcccc ggattgccta tatcgagcac
     2341 aggtgtgcat cgctcttcga gcataccggg attcttccct catggatctc ggatggacag
     2401 gtcgtcttct tcttcctttg tcctgaacag aacgtgggat atgagcttga ccactgcctc
     2461 gagagatacc ggatacggta tccattgctc cttgcccggc aggaggaggg agccggcttc
     2521 atacatcgac gttgaacagc ccccgtatca gcaggccgat aaggaacgaa actcccgcaa
     2581 taccaagtga tatggcaacc atctcaagaa atctcctcca gaatgggaga ccgcctgcaa
     2641 ccgacgtata gaatgtgaaa cagacgataa tggcaaccgc agtgatcaga gagacgaaga
     2701 tggcgctgaa cgggttgccc atcaggagga acggtacgac gaggagaaag accgtgaaaa
     2761 tatatgcgcc ccccgtgtag agcgcagctt ttgcaggctc ccggcctgtc tggtccgcct
     2821 tctgtgagag atactctgat gacgccatgg agagtgcagc tgccacaccc atgatacacc
     2881 ccactgctgc aatgacagtc gtatcctgga gagcaaacgt gaatccggca aggctccccg
     2941 tgaactccac cagggcatcg tttaatccaa gcacgatcga tcccacgtac cggagtctgt
     3001 cctcctcaag catgccgagg atttccatct cgtgctgctc ttcttcggca atgataccct
     3061 ccacttcagg aattgttccg acaagggtgc catagtcctg aattgcggaa ttctcccggt
     3121 tctccataag cttggccgca aaggtatacc caaggagacg ggcgatgatg tagtaatacc
     3181 ataccctggc gactgcaggt cctgcaacct ttcccgtgta atgttcccat attctggaat
     3241 ggccgagttc ctggtcagcc atcctcaaga ggacagatgc attgtgagga tcgcgaaccg
     3301 cttttgcaag catgcgg
//
LOCUS       DAOQVJ010000135         3311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_33081_length_3311_cov_5.075246, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000135 DAOQVJ010000000
VERSION     DAOQVJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33081_length_3311_cov_5.075246"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKL26_03880"
     CDS             complement(<1..223)
                     /locus_tag="PKL26_03880"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNS82868.1"
                     /translation="MPGFDGTGPRGSGPAAGRGLGPCGRGMAFQRAYPFGYGCGFGRG
                     RGFGRGYRMGYRLTYSAPAPLNTDAQKQFI"
     gene            523..1188
                     /locus_tag="PKL26_03885"
     CDS             523..1188
                     /locus_tag="PKL26_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82869.1"
                     /translation="MNGRTTSYSDREIIGRWAILKRNPAIDHILAGRGLAPTGGEGVI
                     GYFYVDHEEGISVRIHALCRIEPGKLPHIAANFEDHGEDCVLRYDEFGEFRLLSTEEA
                     NNLLLSDDQRWYIFEDQRWFIYYDPEKLHEIRNRVDLDRFRAPGYFDDVSVILIARDQ
                     ERIPEVVWVRLEELSADGKSFQGILLNEPDMDFGVHEGDMLTVRFAEHEEGRFLVAQT
                     GPA"
     gene            complement(1241..1732)
                     /locus_tag="PKL26_03890"
     CDS             complement(1241..1732)
                     /locus_tag="PKL26_03890"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNS82870.1"
                     /translation="MNAIILIGMPGAGKSTVGVILAKALGLKFIDTDIMIQERTGKLL
                     QEILDWEGPRGFERIEEETILSLHPSHAVIATGGSVVCSRAAMAHLKSMGVVVYLKIS
                     YTEMERRIRNITTRGIVLLPGQSLQAMYDQRAPLYQKYADLTIECLDKDFETVVGKVI
                     AGL"
     gene            complement(1776..>3311)
                     /locus_tag="PKL26_03895"
     CDS             complement(1776..>3311)
                     /locus_tag="PKL26_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82871.1"
                     /translation="AAAPGIYECRLVDAHGGIHYGFVHVGMIPGTGNSIASLVDVTAL
                     TQAQEDLKASERKIREILDHLPDLIVVHREGNILYVNPAMTESMGWQPDEVVGQPILK
                     FIAPEFHAAVQDAARLRLESGIDSPYTLDLINRDKKPLTVSVRGTVISLSGSPAIMNI
                     LTDITTVRNAEKALEESERKYRMLVENTNDIIYTTDLAGQITTISPQIRRYGYSPEEI
                     VSENLTSFIVEEDLPRVIADFEKTVSTGQSTLTIFRVRDRSGTIHWFEDNGSALTDAS
                     GNVIGISGILRDITDRRTADDALVRANRQLNLLGGITRHDILNKVSVILGYLEIAKES
                     VKDPELGGYLEKVESATRAIREQIEFMRLFRDIGTRAPEWISLDSALPHRHVPATISF
                     HAEVIGVDILAEPMVERVFYNLLDNSVRHGGRVSAIRVSAYRSGDSLVIVWEDNGVGI
                     ASGEKERIFDRGVGKNTGLGLFLAREILSLTGITIRETGYPGTGARFEITVPRGNFRM
                     AAE"
BASE COUNT          706 a          944 c          892 g          769 t
ORIGIN      
        1 cgatgaactg tttctgcgcg tccgtgttca gaggtgcagg cgcagaatac gtgagtctat
       61 accccatccg gtatccacgt ccgaagccac gaccacgtcc gaatccacag ccatatccga
      121 agggatacgc tctttggaac gccatccccc tgccgcaggg gccaagcccc cttcccgcgg
      181 cgggtccaga cccgcgtggg ccggttccat caaatccagg catctttttc gcctcgcttt
      241 ttgaatatat attcataatg gcataatggt ttgggtttcg gcacatgtgc gaatcgcggt
      301 gcattcccaa aatgcgtgaa aaggggggaa ggtttcacct tctcttttcg tatcatggca
      361 ttgatattca cgacaccctg tcaatcagga ttcccttctc aactgaagaa gccggccatt
      421 ctaccatgcc cggctctcat ctaacagggg caggctctgg ctgtccatgc atattccgaa
      481 accactttca cgcttcccat gaagaggatg taacgacgca ctatgaacgg gagaacgaca
      541 tcctattcgg accgggagat catcggcagg tgggcgattt taaagcgaaa tccagccatt
      601 gaccatatcc tggccggcag gggactggcc cccactggag gagagggggt aatcggctat
      661 ttttacgtgg atcacgagga ggggatcagt gttcgcatcc acgcgctgtg ccggatcgaa
      721 cccggaaaac tccctcatat tgccgcgaat ttcgaggatc atggtgagga ttgcgtcctc
      781 cgttacgatg aattcgggga gttccggctt ctttcgacag aagaagcaaa caatctcctg
      841 ttatccgacg atcagcgatg gtacatattc gaggaccaga gatggttcat ctattacgat
      901 ccggaaaaat tgcacgagat acgaaacagg gtcgatctcg acaggttcag ggcaccgggg
      961 tattttgacg atgtatctgt gatcctgatc gcaagggacc aggaacggat ccccgaagtg
     1021 gtatgggtcc gcctggaaga gctctcggcc gacgggaaat cattccaggg aatactcctg
     1081 aacgaacccg atatggattt tggcgtgcat gaaggggata tgcttacggt tcgtttcgcc
     1141 gagcatgagg aagggcgttt ccttgtcgcg caaacaggac cggcgtgaat gagatgtccc
     1201 cctcataaaa gaccgggata tccgtcatca cgcggattct tcacagcccg gcaatcacct
     1261 ttccgaccac ggtctcaaag tccttgtcca aacattcaat cgtgagatcg gcatatttct
     1321 ggtagagtgg agcccgctgg tcgtacatcg cctgcaagct ctggccaggg aggagaacga
     1381 tgccccttgt cgtgatgttt ctgatcctcc gttccatttc cgtgtaggat atcttcaggt
     1441 acaccaccac tcccatggat ttcaggtgcg ccattgccgc cctgctgcat accacgctcc
     1501 cacctgtcgc aatcaccgcg tgagaagggt gcagggagag gatcgtctcc tcctcgatcc
     1561 gctcaaatcc gcgggggcct tcccaatcga ggatctcctg gagcaacttt ccggtccgct
     1621 cctgtatcat gatgtccgta tcgataaact tcagcccaag cgcctttgcg aggatcacgc
     1681 cgacagtgct cttccctgct ccgggcatgc cgatgaggat gatggcgttc atgcgaatgg
     1741 aaaacccctt ccgtgaccct tgcatccccc gtgccctact ctgcagccat tctgaaattt
     1801 ccccggggca ctgtaatctc gaaccgtgca cctgtgcccg ggtaaccggt ttccctgatg
     1861 gtgatcccgg tcagggagag gatttcccgc gcaaggaaca ggccaagccc ggtattcttc
     1921 cccacgcccc ggtcaaagat cctctctttc tccccagagg cgattccaac cccgttgtct
     1981 tcccaaacga tgacgagact gtcgccagaa cgatatgcag ataccctgat cgcagaaacc
     2041 cgccccccgt ggcggacaga gttgtcgaga aggttgtaga aaacccgttc caccattggc
     2101 tctgccagga tatcgacccc tattacctct gcatggaagg aaatggtggc agggacatga
     2161 cggtgaggaa gtgcggaatc aagagagatc cactccggag cccgggtccc gatatcccgg
     2221 aacaacctca tgaactctat ctgttcgcgg attgcgcggg tggcagactc gaccttttcc
     2281 aggtatccgc ccagctccgg atccttcacg ctctcctttg caatctcaag gtatcccagg
     2341 atgaccgaga ccttgttgag gatatcatgg cgcgtgatgc ccccgagcag gttcagctgg
     2401 cggtttgcac gcacgagcgc gtcgtcggcc gtcctgcgat ccgtgatgtc acggagaatt
     2461 cccgatattc caatcacatt ccctgatgca tcagtcaatg cactcccatt gtcttcgaac
     2521 cagtgtatgg tgccggatct atcccttacc ctgaagatcg tgagggtcga ctgcccggtt
     2581 gagaccgtct tctcaaagtc agcaatcacc ctgggcaggt cttcttcgac aataaatgat
     2641 gtcaggttct cggatactat ctcttccggg gagtacccat accgtcggat ctgggggctg
     2701 atagtcgtga tttgccctgc gagatccgtc gtgtagatga tatcattggt attctccacc
     2761 agcatacggt atttcctctc gctctcctcg agggcttttt cggcattcct gacagtggtg
     2821 atgtcggtca ggatgttcat gatcgcagga gacccggata agctgatgac agtcccgcgg
     2881 acgctgaccg tcaggggttt tttgtcccgg ttaatgagat cgagggtata tggactgtca
     2941 atcccggatt caaggcgcaa gcgggcagcg tcctgtactg cggcatggaa ctcgggagcg
     3001 atgaacttca ggattggttg gcccaccacc tcatcgggct gccaccccat ggactcggtc
     3061 attgcggggt tgacgtaaag gatattcccc tcgcgatgca ctacgatgag gtcgggaagg
     3121 tggtcgagga tctcgcggat ctttctttcg cttgccttga gatcttcctg ggcctgcgtg
     3181 agtgcggtca cgtccacgag cgatgcgatg ctgttgccag tccctgggat catcccgaca
     3241 tggacaaagc cataatggat gccaccgtgt gcatcgacca gcctgcattc atagatgccc
     3301 ggtgcggcgg c
//
LOCUS       DAOQVJ010000136         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_33332_length_3294_cov_4.050942, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000136 DAOQVJ010000000
VERSION     DAOQVJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33332_length_3294_cov_4.050942"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3090
                     /locus_tag="PKL26_03900"
     CDS             <1..3090
                     /locus_tag="PKL26_03900"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS82872.1"
                     /translation="QNNYAWAVAEFNGDLYVGTGRNIPYFVAQAMKARGAFPANWTLS
                     FLTTPSGSPPPPLVLPNHTPPSQEDVIEWSNDMRAEIWRYSGDSWERVHQATTFINPA
                     NGYTYPEGIGYRAMTTFSDVNGTEGIYAGVGFGFGPILVVGSTDGDTWVPIRTSSIPS
                     RDTRAMISHNGRLYMGTGEGIYATGSPSPHEDNWVKVADFQTSSLESYNGYLYAGTGN
                     PIGPSETNGFEVWRSTTASPEGPDDWVKVVSGGAGDAWNVLAATMREYDGDLYVGSMN
                     LPFGTGTEGVKGFDLIRLDSDDSWDLIVGNIHPRIPTYPRGLPMSGWPSGYANPFNLY
                     AWSMEEYHDDLYLGSFDIFSFGRFIEEVPGGTDILFEALESLERGEMSGEFSEELEVL
                     RDMDLEHMEEVDLALIIKLLAIHFGGADLWRSQDGVHWVPMNLNGLGDPDNYGLRTMR
                     ATDDGMIIGTANPFAGCQVWVATTPDPEPPVADFFAIPRSGDEPLSVIFADQSTGGHL
                     SYYWDFGDGTYSTKKENVHTYVSAGTYNVTLTVTNSAGSSTECREKYIKVTKDEPLKA
                     NFTSNLTIGAVPVTVQFYDTSTGSPSSWSWVFQKDSYYLLRDAATKQAIPYLGNEFSA
                     EKDPVVIYTSPGNYSVSLTVSRTGETDTVTKEGYIQVDPPAPEADFNAYPMEGDAPLE
                     VEFWEMVPYSWCYDEFLWDFGDGTTGSGSWLYHTFDAPGLYNVTLTVTSAYGNSTLTK
                     EGFINVTQSLPPVPDFEASPLSGSAPLGIAFTDTSTGLVTSRLWDFGDGTNEWRNEST
                     DVDHTYPFPGPYTVSLTVGNAAGQETVTKPGYIQVSSSGAPPMAFFTMRPMSGIAPLT
                     VAFTDRSMGTPLAWEWNFGDGTTSTEQNPVHTYTGTGTYTVTLRVFNHGGSNSHSSYV
                     FVRSASTVFLPVTTSSSGTAAPTATATPAPPLMPGRAPISFFMVSKSIGFAPMTVAFT
                     DMSFNGPTSWDWNFGDGQTSTIRSPSHTFIIPGTYSVSLKVANTHGESTTSRRIYVR"
BASE COUNT          715 a          951 c          926 g          702 t
ORIGIN      
        1 cagaacaact acgcgtgggc agtcgccgag ttcaacggcg acctgtacgt ggggacaggc
       61 cggaacatcc cgtattttgt tgcccaggca atgaaagcga gaggggcatt ccccgccaac
      121 tggaccctct cctttttaac aaccccgtcc ggatcgcccc cgccgccgct tgtgcttccc
      181 aaccacacgc caccatccca ggaggacgtg atcgagtggt caaatgacat gcgggcggag
      241 atatggaggt attccgggga ctcctgggaa cgcgtgcacc aggccaccac gttcatcaat
      301 cccgcgaacg ggtacactta tcctgaaggg atcggatacc gggccatgac aacgttttcc
      361 gatgtcaatg ggacagaagg catctacgca ggagtcgggt tcggattcgg ccctatcctt
      421 gtcgtcggtt ccaccgatgg tgatacctgg gtgccaatca ggacctcgag tattcccagc
      481 cgggacacga gggccatgat ctcccacaat ggcaggctct acatgggaac aggtgaaggg
      541 atttatgcca ccggttcgcc ctctccccac gaggacaact gggtgaaggt ggcggatttc
      601 cagacctcat ccctcgaaag ttataacgga tacctctatg cagggaccgg gaaccccatc
      661 ggtccttcgg agacgaacgg ctttgaggta tggaggagca ccacggcatc acctgaaggc
      721 ccggacgact gggtgaaggt ggtatccggc ggggccgggg acgcctggaa cgtccttgcc
      781 gcgacaatga gagagtacga tggagacctc tacgtgggtt cgatgaacct cccgttcggc
      841 accgggaccg agggtgtcaa ggggttcgac ctcatccgcc ttgattccga tgattcctgg
      901 gacctgattg tcgggaacat ccatcccagg atcccgacct acccgcgtgg ccttccgatg
      961 agcgggtggc cgtccgggta tgcgaacccc ttcaacctct atgcatggtc gatggaagag
     1021 taccacgacg acctgtacct cggttccttt gatatcttct cctttggccg attcatcgaa
     1081 gaggtccctg gaggcactga tattctgttt gaggcactcg aatcgctcga aagaggggag
     1141 atgtccgggg agttttccga agagctcgaa gtgctccgcg atatggacct tgaacacatg
     1201 gaagaggtag acctcgcttt gatcatcaag ctccttgcaa tccacttcgg gggagcggat
     1261 ctctggagga gccaggacgg tgttcactgg gtcccgatga acctgaacgg tcttggagac
     1321 ccggacaact acgggctccg gacgatgcgt gcgacggatg acgggatgat tatcgggacc
     1381 gcaaacccgt ttgcggggtg ccaggtatgg gttgcgacga ctcctgatcc ggaaccgccc
     1441 gtcgccgatt tcttcgcgat tccacgaagc ggcgatgaac cgctctcggt catatttgcg
     1501 gaccagtcaa caggaggtca tctctcgtat tactgggact ttggtgacgg gacgtattcg
     1561 accaagaagg aaaatgtaca tacgtacgtc tcggccggaa cctacaacgt gacacttacg
     1621 gtcacgaact ccgccgggtc gagcacagaa tgcagggaga aatatatcaa ggtcacaaag
     1681 gatgagcccc tgaaggcgaa tttcacctcc aacctcacca ttggggcagt ccctgtcacc
     1741 gtccagtttt atgatacttc aactggaagc ccgagttcat ggtcatgggt gttccagaaa
     1801 gactcgtact acctgttgcg cgatgccgca acgaaacagg caattcccta tttaggcaac
     1861 gaattctcgg ccgagaaaga cccggttgtc atctacacct cccctgggaa ctactcggtg
     1921 agccttaccg tcagcaggac cggtgagaca gacaccgtca cgaaagaggg gtacatccag
     1981 gtcgatcccc ctgcaccgga agcagacttc aatgcatacc caatggaggg cgatgcgccc
     2041 cttgaggtag agttctggga gatggttcca tattcctggt gttatgacga attcctctgg
     2101 gactttggtg acgggacaac agggagcggg agctggctgt atcatacctt tgatgccccc
     2161 ggcctctata acgtcactct gaccgtaacg agcgcgtacg gcaacagcac gctcacaaag
     2221 gaagggttca tcaatgttac gcagtccctg cccccggtcc ctgacttcga ggccagcccg
     2281 ctgtcaggga gtgcgccact tggcattgcc tttaccgaca cctcgacagg cctcgtcacc
     2341 tcccggctgt gggacttcgg ggatgggacg aatgaatggc gcaacgagag cacggatgtc
     2401 gatcacacgt atcccttccc cggcccctac accgtctcgc tgacggttgg caatgccgcg
     2461 ggacaggaga cagttacaaa gccaggatac atccaggtgt cgtcatccgg tgcacctccc
     2521 atggcattct tcacgatgag gccgatgtct ggtattgctc cgttgaccgt tgcctttact
     2581 gaccgctcga tggggacgcc acttgcgtgg gagtggaact tcggggatgg aaccacctcc
     2641 acggagcaga accctgtgca cacctatacc gggaccggga cttacacggt caccctgaga
     2701 gtcttcaacc atggcgggag caattcgcac tcttcctatg tcttcgtgag atcggcgtct
     2761 accgtgttcc tgcccgtcac cacgtcttct tcgggaactg ccgctcccac ggccactgca
     2821 acgcctgcgc ctccgctgat gccgggccgg gctccaatct ccttcttcat ggtgagtaaa
     2881 agcattgggt ttgccccgat gactgttgcg tttactgaca tgtccttcaa tggcccgacc
     2941 tcgtgggact ggaactttgg cgatggccag acctcgacta tcaggagccc gtcccacacg
     3001 ttcataattc cggggacgta ttcggtttcc ctcaaggtgg cgaatacgca cggggagagc
     3061 accacctcac gccgcatata cgtgaggtga tggtcggact cctcccttat ttttgcgagg
     3121 atttcccggg ttgcatcgtg agatacctct gcatgctgtg gtaattttcg tcaaccaatt
     3181 gaccttacac ataggtatat aagttccatc gcgtattctc ctgcagtatg aacatagtag
     3241 cagatctcat gaaacaggta gcgacgggag acaatctctc catgatcagc aaat
//
LOCUS       DAOQVJ010000137         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_34184_length_3241_cov_4.493409, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000137 DAOQVJ010000000
VERSION     DAOQVJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34184_length_3241_cov_4.493409"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PKL26_03905"
     CDS             complement(<1..305)
                     /locus_tag="PKL26_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNS82873.1"
                     /translation="MATPNDDGCTLTGALSVTTEINRCITLIHGPAGCAHHNFSLLHA
                     LHAARDLCIPPPVISTDMTEKEVIFGGEHLLEESLCRALKRKPELICVLSTCVAATIG
                     "
     gene            complement(289..1107)
                     /gene="cfbC"
                     /locus_tag="PKL26_03910"
     CDS             complement(289..1107)
                     /gene="cfbC"
                     /locus_tag="PKL26_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HNS82874.1"
                     /translation="MKQIALYGKGGIGKSTTSANLSAALSEQGLDVLQIGCDPKRDST
                     RMLMQGRLIPTVMDLVRERGDDRSLTIDDVVHSGYHGVRCVEAGGPEPGVGCAGRGII
                     ATFQILERLGALRADVVVYDVLGDVVCGGFAMPMREGYAEEVYLVTSGELMSLYAANN
                     ICKAIARLSSRARSRCRLGGVICNAKNLPREEELVTEFAEKIGSSMIQFIPRDQVVQH
                     AELNKKTVVQYAPDSGQAGTYRSLARRIMENRRLDVPSPLEIEDLEELAYGYAE"
     gene            complement(1188..2666)
                     /locus_tag="PKL26_03915"
     CDS             complement(1188..2666)
                     /locus_tag="PKL26_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNS82875.1"
                     /translation="MPTILVRMGRIRYFSAVLGELGEMLVFISPLTLLPLPVALIFRE
                     WEMLVPLALVPAAFFCSGFLLNYLPRKKEDVRFSAALCSVALVWLAFALISCIPFMLV
                     LDISFTDALFESMAGWTGTAFSLLRGLDSLPESLLFWRTYMQWVGGLAVISLSLTLAF
                     RSGLDTSPLFRAERRAERILPSVIANGKEIWAVYILLTVVSAGLIMVARVPLWDALNL
                     ALSGISTGGFIPRAGGILSYQNPFLEAVLIPVMIFGSTPFTLYYMTYRSRKVSFFSEE
                     QVRLLFIFLIFGALIVIADLFFLAGLPIGESFRQGIFMTTSAISTTGFQNGNPHLYPA
                     VTAVFLMMLIFIGGSSGSAAGGIRLSRIALGYRGIIWWFRRAFVRSRVLVPFKYEGRN
                     IPVEVAEPELSKSMLVIILSVITVFIATMVVLQVHITSFGLTDIVFEVVSALSSSGMS
                     TGYVNPSMPLLSKWVFIVVMWVGRLEIMPVIVLFMGLVRGRD"
     gene            complement(2711..>3241)
                     /locus_tag="PKL26_03920"
     CDS             complement(2711..>3241)
                     /locus_tag="PKL26_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82876.1"
                     /translation="DVKREWKLKRDMGARGYTEQEVRREMAMRESDYQSYVAPQRKYA
                     RGIIGISFSQFGRSLGWKENIYRVSLALEPLSGMNRRVWMQLDLGTVLAGHSRPCSVK
                     YLPVIEGGRLMGSIELDGGFPCESADILSTLLQGETGVDPRDFLPHCPLLTPTDLIQM
                     IVCWRIISHRRSMQVR"
BASE COUNT          747 a          965 c          888 g          641 t
ORIGIN      
        1 cctatcgttg cagcgacgca ggtggagagg acacagatca gttcaggttt ccttttcaac
       61 gcacggcaga gagactcctc cagcaggtgt tcgcccccga aaataacctc cttctcggtc
      121 atgtcggtag atatcacagg cggagggata cagaggtccc gtgcggcatg aagtgcatga
      181 aggagtgaaa aattgtggtg ggcacatcct gcaggcccgt gaataagtgt gatgcacctg
      241 ttgatctcgg tggtgaccga gagcgctccg gtaagggtgc acccgtcatc attcggcgta
      301 gccatatgca agttcttcca agtcctctat ctccagggga gatgggacgt ccaggcggcg
      361 gttctccatg atccgcctgg caagcgaccg gtatgtcccc gcctggcccg aatcaggagc
      421 gtattggacc accgtcttct tattcagttc cgcgtgctgg accacctggt cccttggaat
      481 gaactggatc atggaactgc cgatcttttc cgcaaattcg gtgacaagct cctcttccct
      541 cgggaggttc tttgcattgc agatcacccc gccaagcctg caccggctcc ttgcccgcga
      601 ggagagccgg gcaatggcct tgcagatgtt gtttgccgcg tatagcgaca tcagctcgcc
      661 tgaagttacc agatacacct cttctgcata cccttccctc ataggcatgg caaaccctcc
      721 gcagaccaca tcgcccaaca catcgtagac cacgacatcg gcacgaagcg ccccgagccg
      781 ctccagtatc tggaacgtgg caatgatacc cctgcccgca catcccactc ccggctccgg
      841 gcccccggct tccacgcacc tgactccatg gtagccacta tgtacaacgt cgtcaatggt
      901 gagagatcgg tcgtcgcccc gttcacgcac aaggtccata acagtgggga tgagcctgcc
      961 ctgcatcagc atcctggtac tgtcccgctt ggggtcgcac ccgatctgca gcacatcgag
     1021 gccctgctcg gagagcgcgg cagagagatt ggcagaagtg gttgatttcc cgataccgcc
     1081 tttcccgtac agggcgatct gtttcatcac ccattattgg acggggttgg ttattagata
     1141 ttctgaagtt aagaaaagtc aggttgtatt tcagcgggtc ccacaggtca gtcccttccc
     1201 ctaacgagac ccatgaacag gacgatcacc ggcatgatct ccaggcgccc gacccacatg
     1261 accacgataa acacccattt cgagagaagc ggcattgatg ggttcacgta tcccgtgctc
     1321 atgccggacg agctcaatgc agagacgacc tcaaagacga tatcagtcaa cccgaacgac
     1381 gtgatatgca cctgtaggac gaccatcgtg gcgatgaaca cggtgatcac cgagaggatg
     1441 attacaagca tgctcttcga gagttccggc tcggcaacct ccacagggat gttccttcct
     1501 tcgtacttga acggaaccag gaccctgctg cgtacaaagg cccgcctgaa ccaccagatg
     1561 attccccggt acccgagggc aattcttgag agccttatcc cccctgcagc gctcccgctc
     1621 gatcccccaa taaagataag catcatgaga aagaccgcag tcaccgcggg atacaggtgg
     1681 ggatttccat tctggaaccc cgtggtgctg atggccgaag tggtcatgaa tatcccctgg
     1741 cggaacgatt cgccaatggg gagtcctgca agaaagaaga ggtccgcaat cacgatcagg
     1801 gcaccgaaga taaggaagat gaagaggagc ctcacctgct cttctgaaaa aaatgagact
     1861 ttcctgctcc ggtaagtcat gtaatagagg gtgaaaggcg tcgatccgaa tatcatcacc
     1921 gggatcagga ccgcctcgag gaaggggttc tgatacgaga gtatccctcc tgcacgcggt
     1981 atgaatcccc ctgtagagat gccggagagg gcaaggttca gcgcatccca caacgggacc
     2041 ctcgccacca tgataagccc cgccgatacc accgtgagaa ggatgtatac tgcccagatc
     2101 tcttttccgt tggcaatcac gctcgggagg atccgttcag ccctcctctc ggccctgaag
     2161 agaggggatg tgtccaggcc tgatcgaaat gctaaagtca gcgagaggga gatcactgcg
     2221 aggcctccga cccactgcat gtaggtccgc cagaagagga gactctcggg gagggagtcg
     2281 agccccctga gcagggagaa cgcggtccct gtccaccctg ccatcgactc gaagagggca
     2341 tcagtaaagg agatgtccag caccagcatg aacgggatgc aggagatgag ggcgaatgca
     2401 agccatacca gtgcaaccga acagagcgct gccgaaaacc ggacgtcctc cttcttcctt
     2461 ggcaggtaat tgaggagaaa accgctgcag aagaacgctg ccgggacaag tgccaggggt
     2521 acgagcatct cccattctcg gaagatcagg gccacgggaa gggggaggag ggtaagcggg
     2581 ctgatgaaga ccagcatctc gcccagctcc ccgagaacgg cggagaaata ccgtatcctg
     2641 cccattctta caagaatggt tggcacgtcc agcctgatgt atatttgcct ggtcccgcgt
     2701 acgccacctt ttaccggact tgcatggagc gccggtgcga gatgatccgc cagcagacta
     2761 tcatctgtat gaggtccgtg ggcgtcagga gcgggcagtg cggcaggaaa tcgcggggat
     2821 cgactcccgt ctccccctgg agcagggtgg aaagtatatc agcagattca cacgggaacc
     2881 cgccgtcgag ttcgatgctc cccatgagac gtcccccctc aataaccggg agatacttga
     2941 cgctgcacgg acgggaatga ccggcaagaa ccgtccccag gtcaagctgc atccagaccc
     3001 tcctgttcat cccggaaagg ggttcaagcg caagggatac ccggtaaatg ttctccttcc
     3061 agccaaggct ccggccgaac tgcgagaacg agatcccaat gattccccgg gcatactttc
     3121 gttgcggtgc aacatacgac tggtaatccg actctctcat cgccatctcc cttcggactt
     3181 cctgctcggt gtatcccctt gcccccatgt ctctcttgag cttccactcc ctttttacgt
     3241 c
//
LOCUS       DAOQVJ010000138         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_34360_length_3230_cov_5.370709, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000138 DAOQVJ010000000
VERSION     DAOQVJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34360_length_3230_cov_5.370709"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..880)
                     /locus_tag="PKL26_03925"
     CDS             complement(17..880)
                     /locus_tag="PKL26_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82877.1"
                     /translation="MAADIPTHETLVSKVLELPGVRFIEPAFADLPEDTFTAWMEDLP
                     ADQGRLLVDEDSDPLAMAFLTGQGWLAGSLHLREPTLALIEHFEEMNGDIFQEDRAVW
                     EEAVREYYSLLLIETTTPALEDLNPARTAQVRDLLAESWGDRAGISCLDCGCGSGLGS
                     FLLRERGMRPLSYDNDASLLSLGLSRGRLLPGETMCIDATYASRYCGGAEAGLGLMFG
                     EINAFNEQDWEMIAAELLALTNDCLVTVGTEREARLIQSWAEARRCRVEVRENDRDPI
                     YDRWVCLIERA"
     gene            complement(887..2122)
                     /locus_tag="PKL26_03930"
     CDS             complement(887..2122)
                     /locus_tag="PKL26_03930"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNS82878.1"
                     /translation="MTAHKILLLVLDGISDRPCPELGNRTPLQAALTPVLDRMARDGI
                     CGIMDTIGPGIRPGSDTSHLSLLGYDPYKYYTGRGPLEAEGSGIHMEPGMIGFRCNYA
                     TLDDGGRVTDRRAGRIHGTEPLSAAIQEGVDLSSLGVEFLFRSGTGHRAALAFRGEDL
                     GVSVTSNDPKKEGVPPLPFSARDGTDADRKTARALQEFVRQADGILKDHPLNASRASQ
                     GLPSASTILIRGAGRMGHFEPFSSRYGLKGCVISAAALITGIGTAVGLTRIEVPEITG
                     SADTNLAGKVRAAIDAFRTVDFVLMNIKGADEAGHDGKARAKMEFIEKIDSALAPLIS
                     LEDCIVAICADHSTPCSVKDHSADPVPLVICGEGVRVDRVTHFDEVSCAEGGLHRITG
                     ASLMPILLDLLNRSPKYGA"
     gene            complement(2239..2832)
                     /locus_tag="PKL26_03935"
     CDS             complement(2239..2832)
                     /locus_tag="PKL26_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HNS82879.1"
                     /translation="MAKKKQAGRRVEGWKAKSWYKVYTPEVLGKTYIGDTIANDAEGV
                     VGRIMQTTLGEIINDYARQNVKMKFRIANVAGDSAYTEFLGHELTRDYMRSMIKRRTS
                     RIDCHVPVMTKDGKKLDLTVTCFTLTRANLSQVHAIRNLISTMVGKIGAESEMNAIIN
                     GIVNGEISREVFKAVKPLFPVRRVEVIKSKVGVAKVV"
     gene            complement(2862..>3230)
                     /locus_tag="PKL26_03940"
     CDS             complement(2862..>3230)
                     /locus_tag="PKL26_03940"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HNS82880.1"
                     /translation="PPVQGRGHHPVRTPGAVPYNPREGGGRMMEIRGGIRTRHRHAGC
                     VAPSLLPDNLQSMHTREEGDLVVTTIEGTSLRSVIASVDDYLMNLAVAEEMCALEGQV
                     RQPGTNGKGMDQGTRESPGE"
BASE COUNT          521 a         1033 c         1047 g          629 t
ORIGIN      
        1 ggggagatcc gccatctcat gcccgctcga tcaggcagac ccagcggtcg tagatgggat
       61 cgcggtcatt ctcccggacc tccacccggc acctccgggc ctccgcccag gactggatca
      121 gccgggcctc gcgctcggtg ccgaccgtga caaggcagtc gttcgtgagt gccaggagct
      181 cggcggcgat catctcccag tcctgctcgt tgaaggcgtt gatctcgccg aacatgaggc
      241 cgagacccgc ctctgcgccc ccacaatacc ggcttgcata ggtcgcgtcg atgcacatgg
      301 tctcgcccgg gaggagtctt ccccgggaga gtccgagtga gaggagggag gcgtcgttgt
      361 cgtaggagag cgggcgcatc ccgcgctccc gcagaaggaa ggagccgagc cccgacccgc
      421 agccgcagtc gaggcaggag atccctgccc ggtctcccca ggattcggcg aggagatcgc
      481 gcacctgggc ggtcctggcg ggatttaagt cctcgagcgc aggcgtggtc gtctctatca
      541 gaaggaggct gtagtactcc cggacggctt cttcccatac ggcgcggtcc tcctggaaga
      601 tgtcgccgtt catctcctcg aagtgctcga tcagcgcgag cgtcggctca cggagatgga
      661 gggacccggc aagccagccc tggccggtga ggaaggccat tgcaagcggg tcggagtcct
      721 cgtcgacgag gagccggccc tggtcggcgg ggagatcctc catccatgcc gtgaaggtgt
      781 cctccggcag gtcggcgaac gcgggctcga tgaaccgcac accgggcagt tccaggacct
      841 tcgagacgag cgtttcgtgc gtggggatat cggcggccat ggttcatcag gctccgtatt
      901 tcggggaccg gttcaggaga tccagcagta ttggcatcag cgaggcgccg gtgatccggt
      961 ggaggccacc ctcggcgcag gagacctcgt cgaagtgcgt tacccggtcg acccgaacac
     1021 cctcgccgca gatgacgagc ggcaccggat cggcgctgtg gtctttcacg ctgcacggcg
     1081 tggaatggtc tgcgcagatg gcaacgatgc agtcctcgag cgagataagc ggcgccaggg
     1141 ccgaatcgat tttctcgatg aactccatct tcgcccgcgc cttcccgtcg tggccggcct
     1201 cgtcggcacc cttgatgttc atcagcacga agtccacggt cctgaacgcg tcgatggctg
     1261 cacggacctt cccggcaagg tttgtatcgg ccgagccggt gatctccggg acctcgatgc
     1321 gggtgagccc gacggccgtc cctatcccag tgatcagggc ggcagcggag atgacgcacc
     1381 ctttgagccc gtaccgggag gagaagggct caaagtgccc catccttccc gctccccgga
     1441 taagaatggt actcgcggag gggaggcctt gagatgcccg cgacgcgttg agggggtgat
     1501 ctttgagtat cccatcggcc tggcgcacga attcctggag tgcccgtgcg gtcttcctgt
     1561 ccgcatcggt gccgtcccgg gccgagaacg ggagcggagg caccccttcc ttcttcgggt
     1621 cgttcgacgt gaccgagacc ccgaggtcct caccgcggaa ggccagggcg gcgcgatgcc
     1681 cggtgccgga gcggaacagg aattcgacgc cgagcgatga caggtccacc ccctcctgga
     1741 tcgccgcgga gaggggttcg gtgccgtgga tcctcccagc ccggcggtca gtgacccgtc
     1801 ccccgtcatc gagcgtggcg tagttgcagc ggaacccgat catcccgggt tccatgtgga
     1861 tcccgctccc ctctgcctcg agcggccccc ggccggtata gtatttgtag gggtcgtagc
     1921 cgagcaggct caggtgcgac gtgtccgacc cggggcgtat ccccggcccg atggtgtcca
     1981 tgatgccgca tatcccgtcg cgggccatcc ggtcgagcac cggagtgagt gccgcctgga
     2041 ggggtgtcct gttcccgagt tccgggcacg ggcggtccga tatgccgtcc aggacgagga
     2101 ggaggatctt gtgcgccgtc atcaccagaa gagtagtctg ttccctgtct tatgcttgac
     2161 ccatcccccg cgctgcagcg gggcaaaaag agataaaaag gcggttgggc ctggatacga
     2221 aggggcccgg tgcccctttc agacaacctt ggccacgccg actttcgact tgatgacctc
     2281 gacccggcgg accgggaaga ggggctttac cgccttgaag acctcgcgcg agatctcgcc
     2341 gttcacgatg ccgttgatga tggcgttcat ctcggactcc gcaccgatct ttccaaccat
     2401 ggtggagatg aggtttcgga tggcgtgcac ctgggagagg ttcgcccggg tgagggtgaa
     2461 gcatgtcacc gtaaggtcga gcttcttccc gtcctttgtc atgaccggga cgtggcagtc
     2521 gatccgcgac gtgcgccgct tgatcatgga acgcatgtag tcacgggtca gctcgtggcc
     2581 caggaactcg gtgtaggccg agtcccctgc gacattggcg atccggaact tcatcttgac
     2641 gttctgccgt gcatagtcgt tgatgatctc accgagtgtg gtctgcatga tcctgcccac
     2701 cacgccctcg gcgtcgtttg cgatggtgtc cccgatgtag gtctttccga gcacctcggg
     2761 ggtgtacacc ttgtaccagc tcttggcttt ccagccttct actctccgtc cagcctgttt
     2821 cttctttgcc atgtattcac cattctgata ggattgttct gttattctcc ggggctctcg
     2881 cgagtcccct ggtccatacc ctttccgttc gttccgggct gccgcacctg tccctcaagg
     2941 gcgcacatct cctcggccac tgccaggttc atcaggtagt cgtccacaga cgcgatcacc
     3001 gaccggagcg aggttccctc gatcgtggtg acgaccaggt ctccttcctc gcgggtgtgc
     3061 atgctctgga ggttgtcggg caagagagac ggcgccacgc accctgcgtg cctgtgccgt
     3121 gtccggatcc cgccacggat ctccatcatg cgcccaccgc cttctcgagg attgtacgga
     3181 actgctccag gcgtgcgcac gggatggtgg ccccggccct gaaccggtgg
//
LOCUS       DAOQVJ010000139         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_34463_length_3224_cov_5.465762, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000139 DAOQVJ010000000
VERSION     DAOQVJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34463_length_3224_cov_5.465762"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PKL26_03945"
     CDS             <1..167
                     /locus_tag="PKL26_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNS82881.1"
                     /translation="TRGYGKHTGFGLFLSREILSITDITIQETGEPGGGARFEIRVPM
                     GKFRHSGTVP"
     gene            638..>3224
                     /locus_tag="PKL26_03950"
     CDS             638..>3224
                     /locus_tag="PKL26_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82882.1"
                     /translation="MSTTGRERGKKPGMDFSFSHYLLFSMILLILVMAMGITYFDYRH
                     AEETYQKSARAMQEQTENDIAKTIRIVDSAYAMYDQTLNLQMMNGFSLFLGEYERAGR
                     DPSRMDLLSLKDEMGNIMDLYIINESMMVEATTYEPDLGLDFSRWPHSYNFLKDLIQK
                     DGFFPDRVVYEVSSGKLRKYAYMPTPDHRYILELGMTEKGIGDRPAIHYQEPLREIAF
                     NNPHILSYRAFDTIGRLIGKGSDTQVKSPGILKQVVEERRTIEVEDSHNNTVIRYLLV
                     DLSEQSYASDTSWIIELVYDQSGIKTQLADLVFYHLLVALIAVILSAGIAIVLSRQLM
                     KPVKRMVDDIDTISKGDLDHTIAHANIREFSRIEESITALVTTLKGMIGRLRQSEAEL
                     KMSEENYRTVVENQTELIARFLPDGTHLFVNDAYCRYFDIPCSELLGKKMWPRIPEED
                     TLLLARHFASLTPENPNSTIEHRILTPGGEVRWLQWNDRAIFGPDGTVTEYQSVGRDV
                     TEKKRIEQDLVESEKKFRDLASLLPQVIFEIDTTGRITYVNQPAFHRFGYSPEELESG
                     INFTQVIKPEERMEAIQNFIGIMEGRKEAGAEYHMVRRDGSELTGMVYSSPILKDGKA
                     AGIRGILVDITQLKQVEEDLRRLNEELESRVADRTRDLEDANRELEAFSYSVSHDLRA
                     PLRAIDGFSSILLTEHAASLDPAVKGLLLRIRSNAQKMGQLIDSILNFSRMSRQPLSK
                     QTVYPGQIVQEVLEELKTMQEGRNVEVRINSLPSCEGDPALIKQVFHNLISNAFKFTR
                     TRERAVIEIGSFKKDGKAVYMVRDNGIGFDMKYADKLFSVFQRLHDEKMYEGTGIGLA
                     IVYRII"
BASE COUNT          804 a          851 c          864 g          705 t
ORIGIN      
        1 tcacacgcgg ctatgggaag cacacgggtt ttgggctttt tctcagccgc gagatcctct
       61 ctatcaccga tatcactatc caggagacag gggagccagg gggaggggca aggttcgaga
      121 tccgtgtccc gatggggaag ttccggcatt ccgggactgt tccatgacgc ctccccccct
      181 ggagaggtgg gcgtgtggcc ccgcctttga agagtgatgt gattcgtttg gatggattgt
      241 ttgaaaatct ccaccgcgtc atggagaaca ccctgccagg tgatatgccc tatccgggtc
      301 gccctcgcct tttttaggaa ttcgccaggt tttcttccaa cggccacgtg tttaaataaa
      361 ttgatcccca attttggttt tctttttgta taccgcaaag gatttgaata tttcttccgc
      421 ccgggcaaca gctttatcca gtctgtggat gagttaagga catcaaaaag atgctctggt
      481 gagaggtaac cggggggtat cgctctcacc ccacatccca tccatgggcc gtggaattta
      541 ttccatgact ggaacgacat gttcggttca tcgtaaatgt ccacaccctt accctcggtt
      601 gcggaaatga aaaagagctg gttgatcgga gcgtaacatg agcacaacag ggcgggaacg
      661 cggaaagaaa cctggtatgg acttttcatt ctcccattat ctcctcttct ccatgatcct
      721 tctcatcctc gtaatggcca tgggaatcac ttactttgac tacaggcatg cagaggagac
      781 ctaccagaaa agtgcccgcg ccatgcagga acagacagag aatgacattg ccaagacgat
      841 caggatcgtc gactctgcgt acgccatgta tgaccagacc ctcaacctcc agatgatgaa
      901 cgggttttct ctctttcttg gggaatacga gcgcgcggga agagatccgt cccggatgga
      961 cctgctttcc ctgaaggatg aaatggggaa catcatggac ctctacatca tcaacgagtc
     1021 gatgatggtg gaggcgacta cgtatgaacc tgatctcggc cttgatttca gccgatggcc
     1081 gcattcttac aattttttaa aggatctcat ccagaaggac ggatttttcc ctgaccgtgt
     1141 cgtgtacgag gtctcatcgg gaaaactcag gaaatacgcc tatatgccga cacccgacca
     1201 tcgctatatc ctcgagcttg gcatgacgga gaagggcatc ggggatcgcc cggccattca
     1261 ttaccaggaa ccgttacgcg agatagcatt caataatcca cacattctca gttacagggc
     1321 gtttgatacc atcgggcggt tgattgggaa agggagcgat acccaggtga aaagcccggg
     1381 aatattgaaa caggtcgtcg aggagaggag aacgatcgaa gtggaggact cccacaacaa
     1441 cacggttatc aggtatctcc tggtcgatct ctctgaacag agttatgcat ctgataccag
     1501 ctggatcatc gagcttgtct atgaccagtc agggataaag acccagttag ccgaccttgt
     1561 cttctaccac ctcctcgtag cgctcatcgc ggtgatcctg agcgcaggga ttgccattgt
     1621 cctttcccgg cagctcatga aacccgtgaa gagaatggtc gatgatatcg atacaatctc
     1681 gaaaggggac ctggaccata cgatcgccca tgcgaatatc cgcgagtttt cacgcatcga
     1741 agagagtatc accgcccttg tcactaccct gaagggcatg atcgggagac tccggcaaag
     1801 tgaggccgag ttgaagatga gcgaagagaa ttacaggacc gtcgtcgaga accagaccga
     1861 actgatcgca cgattcctgc cagacgggac gcacctgttc gtcaatgacg cctattgcag
     1921 gtacttcgac atcccgtgca gcgaattgct ggggaagaag atgtggcccc gaatcccgga
     1981 agaggacacc ctccttctcg cacgccactt tgcatcgctc acaccagaga acccgaactc
     2041 taccatcgaa caccgcattc tcacccccgg cggcgaagtg cgctggctcc agtggaatga
     2101 ccgggcaata ttcgggcccg acggaacggt taccgaatac cagtcggtgg gaagggacgt
     2161 gacagagaag aagcggatcg agcaggacct tgtggagagc gagaagaagt tccgcgatct
     2221 tgccagtctt cttccccagg tcatcttcga gattgatacc acaggaagga taacctatgt
     2281 aaaccagccc gcattccatc gctttgggta tagtcccgaa gaacttgaaa gcgggatcaa
     2341 tttcacccag gtaatcaaac cggaagaacg catggaggca attcagaact tcatcggaat
     2401 aatggaaggc cgcaaagagg cgggcgctga gtatcacatg gtccgcaggg acggatctga
     2461 actcactggc atggtctact cctcaccgat actcaaggat ggcaaagcgg ctggcatccg
     2521 gggaatcctc gtcgatatca cgcagctcaa gcaggttgag gaggatctcc gccggctgaa
     2581 cgaagaactg gagtcccgcg tggcagatcg taccagagat ctcgaggatg caaaccggga
     2641 actggaggca ttctcatact cggtgtccca tgacctccgt gcgcctctgc gcgcaatcga
     2701 cgggttctct tccattctcc tgaccgagca cgcagcctcc cttgatcccg cggtgaaagg
     2761 gcttctcttg aggatccggt ccaatgcaca gaagatgggg cagctgatcg actccatcct
     2821 gaacttctcg cggatgtccc ggcagcctct ctcgaaacag acggtgtacc ccggccagat
     2881 cgtgcaggaa gtgctggagg aattaaaaac aatgcaagaa gggcggaatg tagaggtccg
     2941 tatcaattcg ctcccctctt gcgaagggga cccggcgctc ataaaacagg tctttcacaa
     3001 cctcatatcc aacgcgttca agttcaccag gacacgggag cgtgcagtta ttgagatagg
     3061 atcttttaag aaggatggaa aagcggtata catggtcagg gacaacggta ttggctttga
     3121 catgaaatac gccgacaagc tcttctctgt cttccagcgg ctccacgatg agaagatgta
     3181 cgaaggcacc gggatcgggc ttgcaatcgt gtaccgtatt atcc
//
LOCUS       DAOQVJ010000140         3221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_34512_length_3221_cov_4.242577, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000140 DAOQVJ010000000
VERSION     DAOQVJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34512_length_3221_cov_4.242577"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..829)
                     /gene="rpl4p"
                     /locus_tag="PKL26_03955"
     CDS             complement(83..829)
                     /gene="rpl4p"
                     /locus_tag="PKL26_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNS82883.1"
                     /translation="MKAQVYSLEGTVAGEIELPPVFSEEFRPDLIRKAVIALQSTRRQ
                     PHGTYPYAGILSSAQSWGSGRGVAQLPRIKGGSRAAKIPQAKGGREAHPPVVQKVLVR
                     HINKKEKQKAFRSALAATVSEDLVRARGHAFTCQVPLVIEDRFGELGKTSEIISALAA
                     VGVLQDVERAKASKKVRAGRGKMRGRRYKQRKSLLIVTADTPLRAAGNLAGVDVATVE
                     QLNCELLAPGTQAGRLTVWTESALVKLGGQ"
     gene            complement(837..1850)
                     /locus_tag="PKL26_03960"
     CDS             complement(837..1850)
                     /locus_tag="PKL26_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNS82884.1"
                     /translation="MPKINQPRKGSLAFSPRKRAKSPVPKYQSWPPYQGNPMLQGFAG
                     YKVGMTHVIMVDDHKNSPTEGKDIMVPVTVVEIPAMKVAAIRAYGRDSYGKHPLTEVW
                     AGDLDDALGRRITPPKEYDGTTAREAIKDAITRGIVAELFAVMHTMPYEVPGVPKKVP
                     DIMEVRVAGGSPEDQFAFLEGLLGKEVDLKSVIQPGAYADITAITTGKGTQGPVKRWG
                     IKLRKRKHSRGGKKRHVGNLGPWNPHHVRWQVPQMGQMGYQQRTEFNKRILKIGENGA
                     EISPAGGFLHYGMLKNPYVLIKGSIPGPVKRLVRIRPAMRQGEHVARQPAIEFVSVES
                     KQG"
     gene            complement(2459..2863)
                     /locus_tag="PKL26_03965"
     CDS             complement(2459..2863)
                     /locus_tag="PKL26_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HNS82885.1"
                     /translation="MKDGLLTDRQKEVLRYRKQGLTQQQIADIIHTSKANVCTIEKSA
                     VENIKRAKETLEFLYTLDATHLCTIPAGTDLLEVAASIYQEAEKIGIKVKYDTISLIN
                     KMRDSAPERFKARFVKENIDVFINEEGDLYFG"
     gene            3080..>3221
                     /locus_tag="PKL26_03970"
     CDS             3080..>3221
                     /locus_tag="PKL26_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HNS82886.1"
                     /translation="MVVKVGVAKLGNIASGVMAELLLDERADREDMQTFMATSGTKLQ
                     PED"
BASE COUNT          594 a          977 c          878 g          772 t
ORIGIN      
        1 ctcgaccacg aactgaagct tgttctggtt ctcgagcatc atcatggcct tctctgtgac
       61 aaacgggtaa cgaagtacca tattactgcc ctccgagttt cacgagcgcg ctctcggtcc
      121 acaccgtgag ccggccggcc tgcgttcccg gcgcaagaag ctcgcagttc agctgctcca
      181 ccgtggcgac gtccacgccg gcaaggttcc ctgcagccct gagaggggtg tctgcggtca
      241 cgatcaggag gcttttccgc tgcttgtagc ggcggcccct catcttgccg cgtccggcac
      301 ggaccttctt gctggccttt gcccgctcca cgtcctgaag cacccccacc gctgcaaggg
      361 cagagatgat ctcgctcgtt ttgccgagct cgccgaaccg gtcctctatc accaggggca
      421 cctggcaggt gaacgcgtgt ccgcgggccc tgaccaggtc ctcgctgaca gtcgcggcaa
      481 gggctgacct gaatgccttc tgtttctctt tcttgttgat gtggcggacc agcaccttct
      541 ggaccaccgg cgggtgagcc tcccggccac cctttgcctg ggggatcttt gctgcccgtg
      601 agccgccttt tatcctcggg agctgggcta cgccgcgtcc tgatccccag ctctgcgctg
      661 atgagagaat gccggcatac gggtaggtcc catgaggctg gcgccgggtg ctctgcaggg
      721 cgatgaccgc cttcctgatc aggtcggggc ggaactcttc agagaatacc ggcggaagtt
      781 cgatctcccc ggcaactgtt ccttcgagtg agtatacctg tgctttcatc gtagtatcac
      841 ccctgcttac tctccacgct cacaaactcg atcgccggct gcctggccac gtgctctccc
      901 tgtctcatgg cggggcggat gcgcacaagg cgcttgacag ggccgggaat ggatcccttg
      961 atcagcacgt aggggttctt cagcatgccg taatgcaaaa agccgcctgc cgggctgatc
     1021 tcggcaccgt tctctccgat cttgaggatg cgcttgttga actcggtcct ctgctggtac
     1081 cccatctgtc ccatctgggg tacctgccag cgcacgtggt gagggttcca tggccccagg
     1141 ttacccacat ggcgcttctt tcctcccctc gagtgttttc gcttacggag cttgattccc
     1201 cagcgcttga cagggccctg cgtccctttc ccggtcgtta tcgcggtgat atctgcatat
     1261 gcaccgggct gtatcacgct ctttaaatcg acctcttttc caaggagccc ctcgaggaac
     1321 gcgaactgat cctcaggaga gccccctgcg accctgacct ccatgatgtc gggcactttc
     1381 tttgggaccc cggggacctc gtagggcatg gtgtgcatca ctgcaaagag ttctgcgaca
     1441 atgccccttg taatcgcgtc ttttatggcc tctcttgcgg tcgttccatc gtactccttc
     1501 ggaggcgtga tcctcctccc gagagcgtcg tcgaggtcgc ctgcccacac ctcagtcaga
     1561 gggtgtttgc cgtaagagtc ccttccgtag gcccggatcg cggcgacttt catcgccggg
     1621 atctccacga ccgtgaccgg gaccatgata tccttcccct cggtcgggct gtttttgtga
     1681 tcatcgacca tgatcacgtg ggtcatgcct accttgtagc cggcaaagcc ctgtagcatc
     1741 gggttcccct ggtaaggagg ccaggactgg tacttcggta ccgggctctt ggccctcttg
     1801 cgggggctga aggcaaggga tccttttctt ggctggttta tctttggcat ggttcaatca
     1861 atcctgcact atatgactca actgtggcca tctcctggcc tgaaccgaac tgatcggttg
     1921 aaatcgccta tatcggccga tatacaccgg agatgagtga tgtgtccggg cccctgccat
     1981 gcaatcggcc ggaggttgcc cgggttcggt accctggctc atgccaaccg attgccgcag
     2041 cggaaaggcg ggatgatacc cgcctttggt gcagtatccc ggaaagacag ccgtttctct
     2101 ggctcctggc cgcctcccct gtgacctgtc tcgcgaaaga gatcctgaaa ctggcgcgca
     2161 acactgccat cctcgtggca ggcaccgggc acatacccca gaggatacgt ccgcccttca
     2221 cgcctctctt cagttccacc atgtcatccg gtggtgattg ctcaccacat actcatgcaa
     2281 aggcacgaag tccctcggaa cccgtgcctc cgacctctga tgagactcca tcccctggcc
     2341 gctatgtgcc gggcctggat ggatcctcat ataggcttcc atagttatac gagagaaatg
     2401 aatataaacc ttgttccatt tcggagagga cgaaccaccg ggccgatagg gggtcttttc
     2461 agccgaagta gaggtccccc tcttcgttga tgaacacatc gatgttctct ttcacaaagc
     2521 gggctttgaa tcgctccggt gctgagtccc tcatcttgtt gatcagcgag atcgtatcgt
     2581 atttcacctt gatcccgatc ttctctgctt cctggtagat ggatgcggcg acttcgagga
     2641 gatccgtccc ggcggggatg gtgcagagat gagtggcgtc cagcgtgtat aaaaattcca
     2701 gagtctcttt tgccctcttg atgttctcaa ctgccgactt ctcgatggtg cacacattcg
     2761 ctttcgacgt gtggatgata tctgcaatct gctgctgggt aagcccctgc ttccggtaac
     2821 gcaggacctc cttctgacga tccgtcagca gcccgtcttt catgatactg gataggagga
     2881 ttggacttaa acacttttcg ttaacttcgt cacctgcacc tccgaaacgg ggggaggcag
     2941 ggtatggcag ggtaggtcca ctctccgatt actcattaaa accaaaaatc gccctgattt
     3001 gctaaaaaaa agatttcaaa acgtttatat tcgcaccggc agatagtaac ctgttataac
     3061 cagaaatgag gtgtattaca tggttgtgaa agttggagtt gcaaaactgg gcaatattgc
     3121 cagtggtgta atggccgagc ttcttctgga cgagagagcc gaccgcgagg acatgcagac
     3181 attcatggcc acgagcggga ccaagctcca gcccgaggac a
//
LOCUS       DAOQVJ010000141         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_34797_length_3204_cov_3.871070, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000141 DAOQVJ010000000
VERSION     DAOQVJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34797_length_3204_cov_3.871070"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKL26_03975"
     CDS             complement(<1..258)
                     /locus_tag="PKL26_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82887.1"
                     /translation="MMIAFDRVSLALGEFALKDISFRVREGDYFFIIGPSGAGKTILL
                     EAIAGLYVPDQGRILLSGNDVSQIPPEKRGVGLVYQDYSLFP"
     gene            complement(242..1078)
                     /locus_tag="PKL26_03980"
     CDS             complement(242..1078)
                     /locus_tag="PKL26_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS82888.1"
                     /translation="MADKGKVSSWVRYRRPDSCLLAFTLIGSTLVIFAFLALATITVT
                     QLADLPFLIQVALDSRVIDSILLTFGAGLAVVGVLMILGTPLAYVLARTHFRLNSLIE
                     ALIDIPLVLPHTVAGIMVYLLFMQRGPLGAPFKDIGIVFEDAFPGIVVAMLFVAMPFY
                     INTVREGFQKVPLHIENVARTLGASRFRAFVLVVLPLSTRHILNGCVLAWGRAISEFA
                     AVIMIAYYPMIVSTLIYYRFTTGGLKESSAIAFLMILASFSVFLVLRIATRYLGRYDD
                     RV"
     gene            complement(1186..2253)
                     /gene="wtpA"
                     /locus_tag="PKL26_03985"
     CDS             complement(1186..2253)
                     /gene="wtpA"
                     /locus_tag="PKL26_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HNS82889.1"
                     /translation="MNRIVKSCVLILIATALVLSCACVTQEQGAPATPAATTAPAGKI
                     TVKVFHAGSLTGPFEKLKTEFETKYPNTEVLLEPGGSVDVIKKVTDLDKPADVVASAD
                     YALIPQYMVPNDADWYLTFAKNQMTIAYTDKSKFASEITADNWYEILGREGVRWAFSD
                     PNADPCGYRTPMVIQLAEDYYKDDTIFDTLVSDHSKITTTKSGNIWTIDATDPSPDGT
                     KLTVRPKSVELVQMLQAGGLDYAWEYRSVSVQNDLKYLELPEAIDLSSVDYAEAYATV
                     QTKAVKGNGTTLYVGAPIVYGVTVPKIAEHPDYGLLFVKMLIGPEGQAILTADGQPPI
                     VPAGGYGTVPSEIAGMVSMKS"
     gene            2513..3136
                     /locus_tag="PKL26_03990"
     CDS             2513..3136
                     /locus_tag="PKL26_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNS82890.1"
                     /translation="MIPLLVEFSKKTVVIFGGGEVGARKAAYFAPEARVHVYSRSFSP
                     GFSGLNVKLDTIDLHGAGDERLPALLQGAFLAIAATPDRELNNRIGELCLERGILFNN
                     AEGDAGDLLIPSVVRGERFLLAISTGGSSPAVSRFLREFLEKFLPDLDQMIALQERLR
                     AYLKECQPDANARKEVVWKVLMDRDVWAALHGGEIAAWSLITRRYLQ"
BASE COUNT          721 a          882 c          915 g          686 t
ORIGIN      
        1 cggaaagagc gaataatcct ggtataccag gccgacaccg cgtttttcgg gagggatctg
       61 actcacgtcg ttcccggaga ggaggatcct cccctggtca gggacgtaga gtccggcgat
      121 cgcttcaagc aggatcgtct tccctgcccc ggacggaccg atgatgaaga agtaatcccc
      181 ctccctgaca cggaatgaga tgtcctttaa agcaaactcg ccgagggcaa ggctcacgcg
      241 gtcaaacgcg atcatcatat ctccccaggt atcttgtcgc aatccgcaac accaggaaca
      301 ccgagaaact tgcgagtatc atcaggaacg cgatcgcact gctctccttg agccctcccg
      361 tcgtgaaccg gtagtagatg agcgtggaga caatcatcgg gtagtaggcg atcatgatga
      421 ctgccgcaaa ctcgctgatc gcacgccccc acgcgagtac acacccgttc aggatgtgcc
      481 gcgtggagag aggaagcacg accaggacaa aggcccggaa ccggctcgcc ccaagggtgc
      541 gggcaacgtt ctcgatgtgg agcgggactt tctggaaccc ttccctgacg gtattgatat
      601 aaaagggcat ggcgacaaag agcatggcca ccacgatccc ggggaacgcg tcctcgaaga
      661 cgatcccgat atccttgaag ggtgcaccca gggggcctct ctgcatgaag agcaggtaca
      721 ccatgatccc ggccactgtg tgcgggagca cgaggggaat gtcaatgagg gcttcaataa
      781 ggctgtttaa ccggaaatgg gtcctggcaa ggacatacgc gagcggagtc ccgagtatca
      841 tgaggacccc caccaccgcg agacccgcgc cgaacgtgag gaggatggaa tctatcaccc
      901 gggagtcgag tgcgacctgg atgaggaacg ggaggtctgc aagctgcgtg accgtgatcg
      961 tcgcgagggc caggaacgca aatatcacga gcgttgaccc gataagcgtg aatgcgagca
     1021 ggcacgagtc cggccgcctg taccttaccc acgacgagac cttcccctta tctgccatgt
     1081 cctgccccgt tccttaaggc caatgcgaat cagaccaatt gagatgcatt atccgctttt
     1141 ttcaatctgc attcatcccc gtctgctggt aaaaaagaga agaggtcagg atttcatgga
     1201 gaccatgccg gcgatctcgg aggggacagt cccgtatcct ccggcaggaa caatcggtgg
     1261 ctggccgtca gcggtgagaa tggcctgccc ttcggggccg atcagcatct tcacgaacag
     1321 gagcccgtaa tcagggtgct cggcgatctt gggtacggtg actccataga cgattggtgc
     1381 tcccacgtac agcgttgtcc cattgccttt cactgccttt gtctggacgg ttgcgtacgc
     1441 ttctgcataa tcaacagagg agaggtcgat tgcttcaggg agctcgaggt acttgagatc
     1501 gttctggacc gagacactcc ggtactccca ggcataatcg aggcctcccg cctggagcat
     1561 ctggacaagc tcgacactct tgggtctcac ggtaagcttg gtgccgtcag gtgacgggtc
     1621 ggtggcatcg atcgtccata tattgccact cttggtggtg gtaatcttgc tgtggtcgct
     1681 cacgagggta tcaaagatcg tgtcgtcctt gtaataatcc tctgcgagct ggatgaccat
     1741 gggggtccgg tatccacacg gatccgcgtt ggggtcggaa aatgcccacc tgacgccctc
     1801 gcggccgagg atctcgtacc agttgtctgc ggtaatctcc gatgcgaact tgctcttgtc
     1861 cgtatatgcg atcgtcatct ggttcttcgc aaacgtgaga taccagtcag cgtcgttcgg
     1921 gaccatgtac tgtgggatca acgcatagtc ggccgatgca accacatctg ccggcttgtc
     1981 gagatcggtc actttcttga tcacgtcaac actgccaccc ggttcaagga ggacctcggt
     2041 gttgggatac ttagtttcga actcggtttt cagcttctcg aacgggcccg tcaggcttcc
     2101 ggcatgaaag actttcactg tgatctttcc tgccggggca gtggtcgcag ccggtgttgc
     2161 cggtgctccc tgttcctgcg tgacacatgc acatgagagt accagagccg tcgcaatgag
     2221 aatgagtaca caacttttca caattcgatt catacaccac ttttacccga gaatctataa
     2281 attactaatc aattgtagta aaaatagtta cttggtataa tcaattgatt taacagttgg
     2341 tgagggatcg agcgagggtg tgtgccaacg tctcgcctcc cctggcagag tgaaggagaa
     2401 cacacgccga accccgccac cggacaattc aatgccatgc gatattcccc cagtacaatt
     2461 ttcatcctga atccaacatc ttaattaaaa aagacagggt agatctatag caatgatccc
     2521 cctcctggtg gaattctcca aaaagacagt ggtgatcttc ggaggcggtg aggtgggagc
     2581 acggaaagca gcatattttg ctccggaagc aagagtgcat gtctacagca ggtcattctc
     2641 tcctgggttc tcagggctga atgtcaagct ggataccatt gacttacatg gagccggaga
     2701 cgagcggctc cctgcacttc ttcagggagc gtttctcgcc attgcggcaa ctcctgatcg
     2761 ggagctgaac aaccgcatcg gggagttgtg cctggagagg gggatcctct tcaacaatgc
     2821 cgagggggat gccggcgacc tgctgatccc ctctgtggtg aggggtgagc gatttctcct
     2881 ggctataagc acggggggat cgagcccggc ggtctctcgt ttcctccgcg agttccttga
     2941 gaagtttctg ccagacctcg accagatgat cgccctccag gagaggcttc gggcatactt
     3001 aaaagagtgc cagcccgacg cgaacgcaag aaaggaggtg gtgtggaagg tgctgatgga
     3061 cagggatgtc tgggcagccc ttcacggggg cgagatagcc gcatggtccc ttatcacgag
     3121 gaggtatctg caatgaacat ggccccctcc tgcctcctca ccccacttgc catcgcgggt
     3181 gtcagccacc acactgcaac ggtg
//
LOCUS       DAOQVJ010000142         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_34964_length_3194_cov_3.412870, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000142 DAOQVJ010000000
VERSION     DAOQVJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34964_length_3194_cov_3.412870"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKL26_03995"
     CDS             complement(<1..897)
                     /locus_tag="PKL26_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNS82891.1"
                     /translation="MARPDISREILEAIFSRDAAPGDITRIKIEVCKKYGAPAVPKNS
                     ALLAAALPGERERLRALLLVKPTRTLSGVAPVAVMTSPSPCPHGKCLPCPGGPDHPFQ
                     SPQSYTGEEPAALRAREHGYDPYSQVRARLEQFELLGHHVDKVELIVMGGTMTARDPE
                     YQEWFVASCIRAMNEYRGHPAGAATDTASVFLQNEMSPVRCVATTFETRPDWCREEHV
                     ERMLALGVTKVELGVQHLDDRILAYNRRGCTVEDTIAANRLLRDAGIKVGFHVMPNLP
                     GSSIERDRWMFQELFENPDFRPD"
     gene            complement(904..1170)
                     /locus_tag="PKL26_04000"
     CDS             complement(904..1170)
                     /locus_tag="PKL26_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HNS82892.1"
                     /translation="MQKEELLHLHMLMIHIKKYYETITSHEIPTKRYNSLHISPVHIH
                     KNKKCHKDAILALGDEIVTHLQSTQMVPVGYATETASEKVAAEH"
     gene            1224..2108
                     /locus_tag="PKL26_04005"
     CDS             1224..2108
                     /locus_tag="PKL26_04005"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNS82893.1"
                     /translation="MNFISDIRHSTAALLGLAIGDAMGAPLEGVVRPAAPVRRMYAGG
                     RIARHSGACTDDTYQALALAESLAACRGFSPPDFMERLVRDYLFFGEWYGPTSGAVFS
                     LVIGGTPLYSAARLVHSRRGWSRSNGSVMRGPPIGVFYFGPEVEAASMACSALTHRDP
                     VAGACSAFVNRMVSDMCRGMPKERAYARALQRCRDAEVAGMLGRWREFPPSPGLDALE
                     ATHAAVSVFMETLSFEEAVPRAVSMGGDSDTVGAIAGALAGAAYGMHSIPEEWIRGLS
                     DSGRVLRAAHALWLASRG"
     gene            complement(2115..2561)
                     /locus_tag="PKL26_04010"
     CDS             complement(2115..2561)
                     /locus_tag="PKL26_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HNS82894.1"
                     /translation="MAEVKPKVTLIGTTLARVDLEFIYEGECAECEGCTVRKACHNLK
                     PGRRYRVVGVRKTHHACPLHLEGATAVEVVESSIAALVSADMAITNTRILFEPSCSRD
                     KCEHFRLCNPEGVIPGEKYVVVRVLGNAPPGCVKGRSLQLVELRAV"
     gene            complement(2568..>3194)
                     /locus_tag="PKL26_04015"
     CDS             complement(2568..>3194)
                     /locus_tag="PKL26_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNS82895.1"
                     /translation="LEAHPPIAIVADTGLIAAAPHRLLASGCADVISNSTAVLDWELA
                     HRLKHEEVSEYAVALSRMTAELLMKNAKGIKPHQEESAWMVTKALVSSGVAMSIAGSS
                     RPGSGGEHKFGHALERLAPGAALHGEACGIGTIIAMYLHGGDWRAIREALRSIGAPIT
                     PADLGIDDEVAIEALLAAKDIRPERFTILDMGLTRESATELVRMLYRE"
BASE COUNT          508 a          996 c         1056 g          634 t
ORIGIN      
        1 gtccggccgg aagtcagggt tttcgaagag ctcctggaac atccaccggt cacgctctat
       61 gctgctcccc gggaggttgg gcatcacgtg gaacccgacc ttgatcccgg cgtcccgcag
      121 cagccggttt gcggcgatgg tatcctccac cgtgcagccc ctgcggttgt aggcgaggat
      181 gcggtcgtca aggtgctgga cgccgagctc gaccttggtt accccgagcg caagcatccg
      241 ttcgacgtgc tcctcccggc accagtcagg ccgggtctca aaggtcgtgg cgacgcatcg
      301 aaccggcgac atctcgttct ggaggaagac ggatgcggta tcggtcgcgg cccccgccgg
      361 gtgcccacgg tactcgttca tggcccggat gcaggaagcc acgaaccact cctggtactc
      421 ggggtccctc gcggtcatcg tccctcccat cacgatcagt tccaccttgt ccacgtggtg
      481 gcccaggagc tcgaactgct cgaggcgcgc ccgcacctgg gagtaagggt cataaccatg
      541 ctcccgggca cgaagcgccg cgggttcctc cccggtatag ctctggggag actggaacgg
      601 gtggtcaggg ccacccgggc atgggagaca cttgccgtgg gggcaggggg agggagatgt
      661 catcacggca actggtgcga caccagagag ggtgcgggtc ggtttcacga ggaggagtgc
      721 cctgagccgc tcccgttcgc ctggcagggc ggcggcaagg agagccgagt tcttcgggac
      781 agctggggct ccgtattttt tgcagacctc tatttttatc cgcgtgatgt ctcccggggc
      841 ggcgtcccgg gaaaaaattg cctcgagtat ctcccgggag atatcggggc gtgccatgca
      901 aggttagtgt tcggctgcga ccttctctga tgcagtctcg gtggcgtaac ccaccggcac
      961 catctgggtg ctctgaaggt gagtgacgat ctcgtcgccc agggcaagga ttgcgtcctt
     1021 gtggcacttc ttatttttgt ggatatgaac aggagagata tgaagggagt tatagcgttt
     1081 tgtggggatt tcatggctgg tgatggtttc gtagtacttc ttgatgtgga tcatcaacat
     1141 gtggagatgg agtaattctt ccttttgcac actatcacct tctgagaatc aacactattt
     1201 tcctgatcaa atatagttta ttggtgaact ttatatccga catcaggcac tccactgccg
     1261 ccctgctggg gcttgcaatt ggcgacgcga tgggtgcgcc cctggagggg gtggtgcgcc
     1321 cggcggcccc cgtcagaagg atgtacgcgg ggggcaggat cgcgcggcat tccggggcct
     1381 gcacggacga cacctaccag gcgcttgcac tcgcggagtc gcttgcggcg tgccgcggct
     1441 tctctcctcc ggacttcatg gaaaggctcg tccgggacta cctctttttc ggcgagtggt
     1501 acggccccac ttcgggcgcg gtcttctccc tggtaatcgg ggggaccccg ctctattccg
     1561 cggcccgcct cgtgcactcc cggagggggt ggagcaggag caacgggagc gtgatgcgcg
     1621 ggcccccgat cggggtgttc tatttcggcc ccgaggtaga ggcagcgtcc atggcctgtt
     1681 cggcgctgac gcacagggac ccggtggcag gtgcctgctc ggcgtttgtc aaccgcatgg
     1741 tgagcgacat gtgccgcggc atgcctaagg agagggcgta tgcccgtgcg ctccagcggt
     1801 gccgggacgc ggaggtggcc ggcatgctgg gcaggtggcg ggaattcccg ccgtcccccg
     1861 gactcgatgc cctggaggcg acccatgccg cggtctcggt cttcatggag accctctcct
     1921 ttgaggaggc ggtgccgcgt gcagtcagca tgggcggcga cagcgacacg gtcggggcga
     1981 tcgcaggtgc gctggccggg gctgcctacg gcatgcattc catccccgaa gagtggatcc
     2041 gcggcctctc tgacagcggc cgtgtgctgc gggcggcaca cgcgctgtgg cttgcgtccc
     2101 gtggctgata ccgatcagac cgccctcagt tccaccagct ggaggcttcg ccccttcacg
     2161 caacccgggg gagcattccc gagcaccctg acgacgacgt acttctcgcc cgggatgacg
     2221 ccctcgggat tgcagagccg gaaatgttcg cacttatccc tggagcagga gggctcaaac
     2281 aggatgcggg tgtttgtgat ggccatgtcc gcgctgacga gggccgcaat cgacgactcc
     2341 accacctcaa ctgccgtggc accctcaagg tgcagggggc atgcgtggtg ggtcttcctc
     2401 accccgacga cccggtatct gcgcccgggt ttcaggttat ggcacgcctt cctgaccgtg
     2461 cagccctcgc actcggcgca ctcgccctcg tagatgaact cgaggtcgac cctcgcgagg
     2521 gtggtgccga tgagggtgac ctttggtttg acttctgcca ttctctctta ctcccggtag
     2581 agcatcctca cgagctcggt tgcagactct ctcgtcagcc ccatgtccag gatggtgaac
     2641 cgctcgggcc ggatatcctt tgcggcaagc agcgcctcga tggcgacctc gtcgtcgatc
     2701 ccgaggtctg caggggtgat gggtgcgccg atggaccgca gcgcctctct gatcgcccgc
     2761 cagtctccgc cgtgcaggta catggcgatg atagtgccga tcccgcaggc ctccccgtgg
     2821 agggccgctc ccggtgcgag ccgctccagg gcatggccaa acttatgctc ccccccagac
     2881 cccggccgtg aggagccggc aatactcatc gcgacccccg aggatacgag cgccttcgtg
     2941 accatccacg cggactcctc ctggtggggc ttgatgccct ttgcgttctt catcaggagc
     3001 tccgcggtca tccgcgagag agcgaccgcg tactcgctca cttcttcgtg cttcagccgg
     3061 tgggcgagct cccagtcgag cacggcggtg gagttcgaga tcacgtctgc acaaccggac
     3121 gcgagaagcc ggtgcggcgc cgccgcgatg agcccggtgt ccgcgacaat cgcgatcggc
     3181 gggtgcgcct cgag
//
LOCUS       DAOQVJ010000143         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_35058_length_3189_cov_3.506063, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000143 DAOQVJ010000000
VERSION     DAOQVJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35058_length_3189_cov_3.506063"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKL26_04020"
     CDS             complement(<1..477)
                     /locus_tag="PKL26_04020"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNS82896.1"
                     /translation="MSVMPSLIRECLTGVPPLVEAVARMEGINPRVVARSVARGRLVI
                     PANPGRSHRPCGIGEGCRVKVNVNVGTSGERCDLSLEKAKARAALENGADALMDLSTG
                     GDLPAVRRMVLSMDAPVGTVPVYEAVRRAGSAADVDADLLFSVIRDHCRDGVDFLTL"
     gene            567..948
                     /locus_tag="PKL26_04025"
                     /pseudo
     CDS             567..948
                     /locus_tag="PKL26_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314732.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     assembly_gap    949..958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <959..1108
                     /locus_tag="PKL26_04030"
     CDS             <959..1108
                     /locus_tag="PKL26_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS82897.1"
                     /translation="GSRADLVRSLERLSSLDIEGLFPGHGSPVERGGKRHILAAKELL
                     RITYA"
     gene            1101..1535
                     /locus_tag="PKL26_04035"
     CDS             1101..1535
                     /locus_tag="PKL26_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HNS82898.1"
                     /translation="MHSGVYCLVLENTPCREQVGALGPVEFPGGFHVYVGSALGPGGL
                     SRVARHIHCARVGIARPRWHIDYLLASDAFILRSVFCIPTEERLECRLASLIPGRPVP
                     GFGCSDCDCASHLFSFSEHPRVHLARAASKLGLSAHSKTIKT"
     gene            1554..2105
                     /locus_tag="PKL26_04040"
     CDS             1554..2105
                     /locus_tag="PKL26_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS82899.1"
                     /translation="MSVLGISGSMRKDGNTSLLVKAVLERCENAGIKTEFISLAGKDI
                     KPCLGCEKCKEKDWCVIKNDDWGEIMEKVLDCEVLVIGSPTYYFDVCGHLKNFIDRSY
                     SLYHRRKLAGRKAVAVAVCANKGGARTIETLEGFLNSHEFSYLGWVRGKGYLEGEITK
                     DQKGLKRAQEIGDKIVQLLKPHD"
     gene            complement(2257..>3189)
                     /gene="cofH"
                     /locus_tag="PKL26_04045"
     CDS             complement(2257..>3189)
                     /gene="cofH"
                     /locus_tag="PKL26_04045"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HNS82900.1"
                     /translation="VRNQNINVTNICINSCGFCGFSRKPKDKDAYHFPAEVVREKAAL
                     ARSRGVTEICTVSGLHPDFDAGSYLEIISCIREGAPGVHIHASNPMEVAYGAGKSGIP
                     TIEMLGLMKEAGLGSLCGTAAEILVDGVREVICPGKVSTKDWVRIIREAHSLGIRSTA
                     TIMYGHCETVEDQARHLALIRDIQDETGGFTEFVPLSFIHRETPLFRKGLARAGATGR
                     EDLLMTAVSRLFLDNIPHIQVSWVKLGLKMAQICLLAGGDDLGGTMFEESISKGAGAV
                     DTDYLDPREMRRMAGDIGRTLKQRTTLYTDPGHT"
BASE COUNT          620 a          984 c          932 g          643 t
ORIGIN      
        1 gagggtgagg aagtccacgc cgtcacggca atgatcccgt atcaccgaga agaggaggtc
       61 agcatcgaca tcggccgcgc tccccgccct ccggaccgcc tcgtaaacgg ggacggtccc
      121 aaccggcgcg tccatcgaga ggaccatccg ccgcacggcc gggaggtctc cccccgtaga
      181 gaggtccatc agggcgtcgg cgccgttctc cagtgccgcc cgggccttgg ccttctcgag
      241 tgacaggtcg caacgctccc ctgaagtgcc gacgttcacg ttgaccttca ccctgcaccc
      301 ttccccgatt ccgcacggcc gatggcttct ccccgggttg gccggaatca ccagcctccc
      361 cctcgcaacc gagcgcgcaa ccacccgtgg gttgattccc tccatcctgg cgacagcctc
      421 caccagggga gggaccccgg tgaggcattc acggatgagg ctaggcatga cagacatttt
      481 ggtctgcaaa cctaaattag tctactatgc cagtccggtg gataccagga ggagggtttc
      541 ttggcaactc ctacgtcgtg ggaggagtgc tcgtggacgc aggggtcctc cccaccgcaa
      601 tagagagata tcgtgaccag attgaggaga tcgtcctcac ccactgccac tacgaccaca
      661 ttgcctatgt gcatgagatc gccagaatct gcgacgcgcc cgtccacatc caccgcctgg
      721 acgcgcccgg cctctcgagc gacgccatct cgctctcgat gcacttcggg gccagcgcgc
      781 cacggcatct tcccgaccgg ctccttgagg aaggggacag ggtaggggat ctcctcgttc
      841 tccatacccc gggtcacacc ccgggcagca tctgcctcct caaagaggac gagcatctcc
      901 tcctctcggg ggacaccgtc tttcctgacg gtggcttcgg gcgcttcgnn nnnnnnnngg
      961 gagcagggca gatcttgtgc gatcgcttga acgactatcc tcccttgaca tcgaggggct
     1021 ctttcccggg cacggctccc ctgtcgagag gggcgggaag cgacacatcc ttgcagcgaa
     1081 ggaattgctg cgcatcacct atgcatagcg gcgtatactg cctggtcctg gagaacactc
     1141 cctgtagaga acaggtgggt gcgctcggac ctgtcgagtt ccccggggga ttccatgtct
     1201 acgtcggttc cgcgcttggg cccggcgggc tttcgcgggt ggcacggcac atccactgtg
     1261 caagagtagg tatagcgcgg ccgcgctggc atatcgatta cctgcttgca tccgatgcgt
     1321 tcatactccg gagtgtattc tgcattccca ccgaagaacg cctggagtgc cggctcgcat
     1381 ccctcatacc ggggaggcca gtccctggat ttgggtgcag cgattgcgac tgtgcctccc
     1441 atctcttctc cttttcggag catccgcggg tgcatcttgc cagggccgca tcgaaacttg
     1501 gcctttctgc tcatagcaaa acaatcaaaa cataggaggt aaactgttag tccatgagcg
     1561 tgctgggtat ttcggggagc atgcggaagg atggcaatac ctccctgctg gtcaaggctg
     1621 tgctggagcg ctgcgagaat gcgggcatca agactgaatt catctccctt gcaggaaagg
     1681 atatcaaacc atgcctcggg tgcgagaagt gcaaggagaa ggactggtgc gtgatcaaga
     1741 acgatgactg gggtgagatc atggagaagg tcctcgactg cgaggtcctc gtcatcgggt
     1801 cgcccaccta ttactttgac gtgtgcggcc acctgaagaa cttcatcgac cgcagctact
     1861 cgctctacca tcgaaggaaa cttgccggga ggaaagcggt cgcagttgca gtctgcgcaa
     1921 acaagggcgg tgcgaggacc atcgagaccc ttgaaggctt cctcaattcc catgagttct
     1981 cctaccttgg atgggtgcgc ggaaagggct acctggaagg agagataacc aaagaccaga
     2041 aaggcctgaa gcgggctcag gagatcggcg ataagatcgt gcagctcctg aagcctcacg
     2101 attaatcctt ttttttatcg gcccaagaga ggagcgtgcc cagcccgaga gcacagcggc
     2161 atttggtgca tatcctgcca catgcaggga tgaggaaacc gcgagagccg ccagggaggt
     2221 tgcgctctgc gcatgggatg gtcaaaatcc tgggtttcag gtgtgcccgg ggtcggtgta
     2281 gagggttgtc ctctgcttga gggtccggcc gatgtccccg gccatgcggc gcatctcccg
     2341 ggggtccagg tagtcggtgt ccacggcccc ggcgcccttg gagatactct cctcgaacat
     2401 cgtcccccca aggtcgtctc cccctgcgag gagacagatc tgggccatct tgagaccgag
     2461 tttgacccac gagacctgta tatggggaat attgtcaagg aagagacgcg agacggcagt
     2521 catcagcagg tcttcccttc ccgtcgcacc cgcacgggcc agccccttcc ggaaaagcgg
     2581 ggtctcccgg tgaatgaaac tgagcgggac gaactccgtg aaccctcccg tctcgtcctg
     2641 gatatccctg atgagcgcaa ggtgcctggc ctggtcctcc actgtctcgc agtgcccgta
     2701 catgatggtc gcagtcgaac ggataccgag tgaatgggcc tccctgatga tccgcaccca
     2761 gtctttggtt gacactttcc ccgggcagat tacctccctg actccgtcaa ccaggatctc
     2821 cgccgcagtt ccgcacagtg agccgagccc cgcctccttc atcagtccaa gcatttcgat
     2881 cgtcggaatg ccactctttc ccgctccata tgcgacttcc atagggttgc ttgcatggat
     2941 atggactcca ggcgcgccct cacgtatgca ggagatgatc tccaggtacg atcccgcatc
     3001 gaagtcaggg tgaaggcctg acaccgtgca gatctctgtc accccccgcg atcgggcgag
     3061 cgccgccttc tccctcacca cctctgccgg gaaatgatac gcatccttgt ctttgggttt
     3121 tctggagaac ccgcagaatc cgcaggagtt gatacagata ttggtgacgt ttatgttctg
     3181 gttgcggac
//
LOCUS       DAOQVJ010000144         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_35160_length_3183_cov_4.958120, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000144 DAOQVJ010000000
VERSION     DAOQVJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35160_length_3183_cov_4.958120"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKL26_04050"
     CDS             complement(<1..735)
                     /locus_tag="PKL26_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNS82901.1"
                     /translation="MIEDFLLLLLFILCLVLSGFFSASEVALVSITRAKVRTLANEHR
                     PGSDALATLKEHPNRFLITILIGNNIVNVAAAAIATAVTISIFGDIGVGIATGVVVIL
                     LLLFGEIGPKIYAARNTERFALAVARPIYYLSRILSPLVWAIEMVAVRRTGEGGFAEP
                     AVTEEEIREWIEVGKEEGAIEQEEREMLYSVLEFGDTTAREIMTPRVDVALIDDQSTL
                     EDALHFFNETGFSRIPVYHEHMDNIIG"
     gene            complement(827..1648)
                     /locus_tag="PKL26_04055"
     CDS             complement(827..1648)
                     /locus_tag="PKL26_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNS82902.1"
                     /translation="MVHFAVSSMFFHEYPLEEIFSYIVEAGFDSVEFWVETPGYWMAG
                     QPEEELMKNLGAFSQLVSPTIHAPVLDLNPTSINPAVAEVSVRYTVEAVCLAARVGSG
                     VVTVHPGRRTAKRPPSAPDYERFARYIRALEEAATGFGVTVAMENMERKINSLLCTPE
                     GMKELLDREPWLGFTLDISHAMGAGPGEAQRYIELCKDRIVNVHVGRAESGKMHLPID
                     GDEEIAGILGQLSASGYDGNLTLEIEDRNFSHDLSSEEKILVLSRELAFMRDCFV"
     gene            1687..2934
                     /gene="xseA"
                     /locus_tag="PKL26_04060"
     CDS             1687..2934
                     /gene="xseA"
                     /locus_tag="PKL26_04060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNS82903.1"
                     /translation="MTWYQKGISDERGGHTTEVLSVSQVSSLVEALLDDTRLQDIWVR
                     GEITNLKLHGSGHCYFSLGEKSRDHSAVLSCVMWRNDARRVECEFRDGMDVVVYGSIG
                     HYAPQGRYQLYVREVRPAGRGEKHLMVEKWKVQLTSEGCFDPQKKRPLPAYPLKVGVV
                     TSETGAVLQDIRNVVSRRYPLELVVSPTAVQGEGAHLEIARALQRVERDVDVIILARG
                     GGSFEDLFPFNYPEVVRAIVSCPVPVVSAIGHEVDTTLADFAADVRAPTPSAAAELVV
                     PDRMALMESLAEYRKSLGNRLLVRLDRAGTDLSEVRERLSPRRVSQRVTAKREDVSVL
                     SGRMERAVLSRIALERERLSAAAGVLAARNPAQLLRRGYCILQKDGRVIHSADEIACG
                     DRLTLRLVDGRAVTRVEEVHHDS"
     gene            2924..3106
                     /gene="xseB"
                     /locus_tag="PKL26_04065"
     CDS             2924..3106
                     /gene="xseB"
                     /locus_tag="PKL26_04065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNS82904.1"
                     /translation="MTHKFEDLMKELKEILAKIEDNQTGLDEAIALYERGAVLVRQAE
                     ELLASAEMKITMLGRD"
BASE COUNT          660 a          908 c          950 g          665 t
ORIGIN      
        1 cccgatgata ttgtccatgt gctcgtgata gacaggaatc cttgaaaacc cggtctcgtt
       61 gaagaaatga agtgcgtcct cgagcgtgct ctggtcatcg atgagcgcca catccaccct
      121 cggggtcatg atctccctgg ccgtggtgtc gccaaactcg agcaccgaat agagcatctc
      181 cctctcttcc tgctcaattg ccccttcttc ctttccgacc tctatccatt cccggatctc
      241 ttcctccgta acggcgggtt cagcgaatcc accttccccc gtcctcctta ccgcaaccat
      301 ctcgattgcc cagacgaggg gggataagat gcgggagagg tagtaaatgg gccttgcaac
      361 cgcgagtgca aaccgctccg tgttccgcgc agcgtatatc tttggcccaa tctcaccaaa
      421 cagcaggagg aggatgacca ccaccccggt ggcaatccct accccgatgt caccgaatat
      481 gctgatagtc accgcggttg cgatcgccgc agccgcgaca ttcacgatgt tgttgccaat
      541 aaggatggta atcaggaagc ggttcgggtg ctccttgagt gttgcaaggg catccgaacc
      601 tggcctgtgc tcattggcaa gagtgcggac cttggccctg gtgatggaga caagcgccac
      661 ctcggatgcc gagaaaaatc cggagagtac caggcagagg atgaacagca aaaggagtaa
      721 aaagtcctcg atcattcaat ccacgccgcc tggacggctt ttttatcgtt gttcatatgt
      781 cctgggacac tacatgtatc gtgctgacct tttataaaac cgtcgattat acgaagcaat
      841 cgcgcatgaa tgccaattcc ctggagagga cgaggatctt ctcctcggat gagaggtcat
      901 gggagaagtt ccggtcttct atctcaaggg tcaggttccc gtcgtagccg gacgccgaca
      961 attgaccgag gatgcctgcg atctcctcgt caccatcgat tgggaggtgc atctttccgg
     1021 actccgccct ccccacatgc acattcacaa tcctgtcttt gcacagttcg atgtagcgtt
     1081 gggcctcgcc tgggcctgct cccatggcat gagagatatc aagtgtgaac ccaagccatg
     1141 gttcccgatc cagcagctcc ttcatccctt caggagtgca gaggagcgag tttatttttc
     1201 gctccatgtt ctccatggcc actgtaacgc caaaacccgt tgctgcttct tcgagtgcgc
     1261 ggatatacct ggcgaagcgt tcataatcgg gagcgctggg aggcctcttt gcggtccgcc
     1321 ggcccgggtg gaccgtgacg acaccgctgc cgacccgtgc cgcaaggcag accgcctcca
     1381 ccgtgtaccg cacggaaacc tccgcgactg cggggttgat tgaggtcggg ttcaggtcga
     1441 gaacaggtgc gtgaatggta ggagagacca gctgggaaaa cgctcccagg tttttcataa
     1501 gttcctcttc gggttgtccc gccatccagt aacctggggt ctccacccag aattccaccg
     1561 aatcgaagcc tgcctccacg atgtatgaga atatctcttc caggggatat tcatggaaaa
     1621 acatgcttga tacggcgaaa tgcaccatga tcctaattca ttacccgccg gccataatag
     1681 ttgttgatga cctggtacca gaaggggatc tccgatgagc ggggcgggca caccacagag
     1741 gtgctctccg tctcacaggt ctcctcgctc gtggaggcgc tcctggatga cacgaggctt
     1801 caggacatct gggtccgtgg agagatcacg aacctcaagc ttcacggctc gggccattgt
     1861 tacttctcgc tcggggagaa gagcagggac cattcagcgg tcctctcctg cgtgatgtgg
     1921 cgtaatgatg caagaagggt ggagtgcgag tttcgcgacg ggatggacgt ggttgtgtat
     1981 ggctcgatcg ggcattatgc tccccagggc cgttaccagc tctatgtgag ggaggtcagg
     2041 ccggccggcc ggggcgagaa gcacctgatg gtggaaaagt ggaaggtcca gctgacctcc
     2101 gaggggtgct ttgatccgca gaagaaacgg ccactacccg cgtatccgct caaggtggga
     2161 gtggtcacct ccgagaccgg cgcagtcctc caggacatcc ggaacgtggt atcccgtcgc
     2221 tatccccttg aactcgtggt ctcccccact gcagtgcagg gagagggggc gcaccttgag
     2281 attgcacgtg ccctgcaaag ggtggagcgg gacgtggatg tgatcatcct tgcaaggggc
     2341 gggggaagtt tcgaggacct gttccccttc aactaccccg aggtggtaag ggcgatagtc
     2401 tcttgtcccg tgccggtggt gagcgcgatc gggcacgagg tggacacaac cctcgcggac
     2461 tttgccgccg atgtcagggc tccgacccct tcagcggcag ccgaactggt ggtccccgac
     2521 aggatggcac tcatggagtc tcttgctgag tacaggaaat ccctgggaaa caggctcctt
     2581 gtgcggctgg accgtgctgg gaccgatctc tccgaggtgc gtgagaggct ctccccccgg
     2641 agggtctcgc agagggtgac cgcgaaaaga gaagacgtct cggtcctttc aggacggatg
     2701 gaaagggcgg tcctgtcgag gatcgcgctg gagagggaac ggctgtctgc cgctgcgggg
     2761 gtgcttgccg caagaaatcc cgcccagctc ctccggaggg gctactgcat cctgcagaag
     2821 gatgggcggg taatccattc ggcagacgag atcgcctgtg gagaccgcct caccctccgt
     2881 ctcgtcgatg gccgggcggt cacgcgcgtg gaagaggtgc atcatgactc ataaatttga
     2941 agacctgatg aaggaattga aagagatcct ggcaaagatc gaggacaacc agactgggct
     3001 cgacgaagcg atcgcccttt atgaacgggg cgcggtcctt gtccggcagg cagaggaact
     3061 cctggcatcc gcggagatga agatcacgat gcttgggagg gactaacctc cctggctgtc
     3121 aggggaccgt gaccgtgagg gtggccacaa cctcgcctcc ctttcggaga gcctctacga
     3181 act
//
LOCUS       DAOQVJ010000145         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_35247_length_3178_cov_4.682997, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000145 DAOQVJ010000000
VERSION     DAOQVJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35247_length_3178_cov_4.682997"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKL26_04070"
     CDS             <1..289
                     /locus_tag="PKL26_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNS82905.1"
                     /translation="LGFIPAAFLSGLGYERKLRLARYGGMAAGLCALYACGVAWLSLS
                     TGLSLEMAILLGAIPFLPGDILKAVVASLATDRIARLKSGMHAGITGEKPG"
     gene            295..993
                     /locus_tag="PKL26_04075"
     CDS             295..993
                     /locus_tag="PKL26_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HNS82906.1"
                     /translation="MIRIHELMHGILDIPSLLIPEGRSAVIGPNGSGKTTLLRLLAGM
                     EEPVQGRITIGGKDPAEVTVGWVHEFPGRNTLFSRVSEELASPLRFRHCTCEEIAQRV
                     KEVSALAGITPLLDREMGALSGGEQVLTALATAILSTPDILVLDEWDSHLDAHTASML
                     QEHLGRAGVSRIVQCTQNMDLASSADAVLFLSDGRVEAFGNPGEVFSGYRESCWYPPS
                     WRLKEWNSSLITQP"
     gene            948..1661
                     /locus_tag="PKL26_04080"
     CDS             948..1661
                     /locus_tag="PKL26_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS82907.1"
                     /translation="MEAKRVELVFDNAAVRRGDATIRVTGRFGPGIHLVSGKVGTGKS
                     TVASMAAGLLDPTEGRITREGVGSVMLSLQFPEWQLTENTIREEIRSYGGDDEAILTR
                     AGIRGREGDDPLSLSRGELKKLNLSCIFGHAWDLLILDEPFGALDCTAKSKLCRWIEE
                     RRSSIVILCTHEQQFLPRVDALWEFQGHDLVFLGTVPGALADWKTAPKTVKALLSRGI
                     VPQNISWNDLEAAACRTRD"
     gene            1643..2296
                     /locus_tag="PKL26_04085"
     CDS             1643..2296
                     /locus_tag="PKL26_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNS82908.1"
                     /translation="MQDPRLRLLAVVALSITAFSGLLGAMLAFIWYLACSGGPGMLRR
                     SWWPLVAFVPLLLVTAALWLTGINWFSYFARLGVVVLIAIFAYQDQKPGEFIQVCAWA
                     LGSRLGFDLGLAGEMGFSSIRYLEGEVRRVRQAYQLKKIRVGVRSLLPISTGLVFGIL
                     RRAEDQADLLLARGYDRGGTACPGFIATGRDYLASGIAVFLFILCFFPVREFFILAQ"
     gene            2359..>3178
                     /locus_tag="PKL26_04090"
     CDS             2359..>3178
                     /locus_tag="PKL26_04090"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HNS82909.1"
                     /translation="MCAASKKKIAITDTTLRDAHQSLIATRLRTEDMLPLARQIDRVG
                     FFSVEAWGGATFDSSIRFLAEDPWERLRALKAELKRTPIQMLLRGQNLVGYRHYPDDV
                     VDRFVERAHANGVDIFRVFDALNDIRNMKRSMEKVKEVGAHLQGAICYTTSPVHSIEG
                     FVEMAKELHSMDCDSICIKDMAGLIMPDAAAELVTGIKDEVDTLVDLHSHSTSGIALM
                     SYEAAIKAGVDILDTAMSPFALGTSQPPTESVVAALSGSPGDTGIDLRVLKDVRN"
BASE COUNT          610 a          896 c         1011 g          661 t
ORIGIN      
        1 gttggggttc attcccgcgg cctttctctc gggtctcggg tacgagagaa aattgcggct
       61 tgcccggtac ggggggatgg cggcaggcct ctgtgcgctc tacgcctgtg gagtggcatg
      121 gctctcgctc tccaccgggc tgtcgcttga gatggcaata ctcctcgggg ccattccctt
      181 ccttcccggc gacatcctga aggcggtggt cgcatccctg gcaaccgatc ggattgcacg
      241 gctgaagtca gggatgcatg cggggatcac gggcgaaaaa ccagggtagg cgcgatgatc
      301 cgcatacatg agctcatgca tgggattctc gatatccctt cacttctcat ccccgagggc
      361 cgttcggccg tgatagggcc gaacgggagt ggaaagacca cacttctccg gctcctcgca
      421 gggatggagg agcccgtgca ggggcgcatc acgattgggg ggaaggaccc tgcggaagtg
      481 acggtcggat gggtccacga gttcccggga agaaacaccc tcttctcccg tgtcagcgag
      541 gagcttgcct cacctctccg cttccggcat tgcacgtgtg aagagatcgc ccagagggtg
      601 aaggaggtct ccgcgcttgc cgggataacc cctcttctgg accgagaaat gggggcgctc
      661 tccggcgggg aacaggtgct cacggcgctt gcgaccgcaa ttttgtccac ccccgacatc
      721 ctggtcctgg atgaatggga ttcccacctt gatgcccata ctgcatcgat gctgcaggag
      781 cacctgggcc gtgccggtgt atcccgaatc gtccagtgca cccagaacat ggatcttgca
      841 tcgtccgccg atgcggtgct cttcctctcg gatggaaggg tggaggcatt cggcaacccc
      901 ggggaggtct tttcaggata cagggagtcc tgctggtatc ccccatcgtg gaggctaaaa
      961 gagtggaact cgtctttgat aacgcagccg taaggcgcgg cgacgccact atccgcgtca
     1021 cagggaggtt cggccctgga atccacctgg tctccgggaa ggtgggtacc ggaaagtcca
     1081 ccgttgcttc catggcggca ggcctccttg atcccacgga gggcaggatt acaagggaag
     1141 gagtggggag cgtgatgctc tcgctccagt ttcctgaatg gcagctgacc gaaaacacca
     1201 tccgcgaaga gatccggtcc tatgggggcg atgacgaagc gattctcacc agggctggca
     1261 tcagggggag ggaaggggac gatcccctgt cgctgagcag gggcgagctg aagaagctca
     1321 acctgtcatg catctttgga cacgcctggg acctgctgat actcgacgag ccattcggtg
     1381 ccctggactg cacggccaag agcaagttgt gccgctggat agaggagagg aggtcatcca
     1441 tcgtgatact ctgcacgcat gagcagcagt tccttccacg tgttgatgcg ctgtgggagt
     1501 tccagggcca tgacctggtc ttcctgggca cagtgccggg agcgcttgct gactggaaga
     1561 ctgccccgaa gacggtgaag gctctccttt cgcgtggcat cgtgccacaa aacatctcct
     1621 ggaacgacct ggaggcggcc gcgtgcagga cccgagactg aggctcctcg cggtggtggc
     1681 gctctccatc accgcattct ctggtctttt gggagcaatg cttgcattca tctggtacct
     1741 tgcctgcagc ggcggaccgg ggatgttgag gaggtcatgg tggcctctcg ttgcgttcgt
     1801 cccgctcctc ctggttacgg cggccctctg gctcaccggt atcaactggt tctcgtactt
     1861 tgcacggctc ggggtggtgg tcctgatcgc gatctttgcg taccaggacc agaagccggg
     1921 agaatttatc caggtctgtg cctgggcgct cgggtcgaga ctgggcttcg acctggggct
     1981 tgccggggag atgggatttt cttccatccg ttatctcgag ggtgaggtgc gccgagtgcg
     2041 ccaggcatac cagctgaaga agataagggt cggcgtgcgc agcctccttc ccatctctac
     2101 aggccttgta tttggcatcc tcaggagagc agaagaccag gcggatctcc ttctcgcccg
     2161 gggatacgac aggggaggga cggcctgtcc agggttcatc gcaaccggaa gggattatct
     2221 cgcctccgga attgcggttt ttctctttat actgtgtttt ttcccagtga gagaattttt
     2281 tatacttgca caatgaagga tttagaggtt tagctctttg ttcgacgctg gagcttgggc
     2341 caagcccgga ggagcccgat gtgtgctgcc agtaaaaaga agattgccat caccgacacc
     2401 acactcagag atgcccatca gtccctgata gccacgcggt tacggacaga ggacatgctc
     2461 ccccttgccc ggcagatcga cagggtagga ttcttttcgg tcgaggcctg gggtggggcg
     2521 acgtttgact ccagtatccg cttccttgcg gaagatccct gggagcgtct gcgggcactc
     2581 aaggcggagc tgaagcggac acctatccag atgctcttac gcgggcagaa cctggtggga
     2641 taccgccatt acccggatga tgtcgtggac cgctttgtgg agcgggcgca tgccaacggc
     2701 gtcgacatct tcagggtatt tgatgccctc aacgacatcc gaaacatgaa gaggtccatg
     2761 gagaaggtca aggaggtggg tgcgcacctc cagggggcca tctgctacac gacaagcccc
     2821 gtccactcga tagaagggtt cgtcgagatg gcaaaggaac tccattccat ggactgcgat
     2881 tcgatatgca tcaaggacat ggcagggctg atcatgccgg atgccgccgc cgagctcgtg
     2941 accgggatca aggacgaggt cgacactctc gtcgacctcc actcccactc cacgagcggg
     3001 atagccctga tgagctacga ggccgcgatc aaggcagggg tggacattct tgatacggca
     3061 atgtccccat ttgcgctcgg gacatcccag ccccccaccg aaagcgtggt ggcagcactt
     3121 tcaggaagtc cgggggacac ggggatcgat ctccgtgtcc tgaaggatgt caggaatg
//
LOCUS       DAOQVJ010000146         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_35301_length_3175_cov_3.829487, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000146 DAOQVJ010000000
VERSION     DAOQVJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35301_length_3175_cov_3.829487"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKL26_04095"
     CDS             complement(<1..605)
                     /locus_tag="PKL26_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82910.1"
                     /translation="MSDHPSTGYDPMTGEEELQGPERYLRPGEELLIYTPDIQIKKFR
                     FDAYLTNTRLFLTDQDEKTPGVTAKEIPIESVADTYLEHSPVQEPILVLSIRTSDDDL
                     RTMKMTFVHNGADRLPQAEEWMHLIMHGHAKRPSGGTAVRPASPQPVPAISPEARALS
                     ETIIYPEKTTVEPGPIPSAGPAVVQEEGRTRIPPVQPRQPEA"
     gene            complement(692..1558)
                     /gene="sucD"
                     /locus_tag="PKL26_04100"
     CDS             complement(692..1558)
                     /gene="sucD"
                     /locus_tag="PKL26_04100"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNS82911.1"
                     /translation="MIYGDKHTGVIVTGATGKQGAFHIALMNEYARQVGGEGVVAGVT
                     PGKAGQEVAGVPVYNSIHDALKEHDASASVLFVPGFAAGDSIMEAASAGLSLVVAITE
                     HIPVHDTMRAISFARIHGCDVIGPNCPGLLSPGEIKMGIMPVHLSSRGHVGVISRSGT
                     LTYEIVDQLTRAGLGQSTIVGIGGDPVIGQTFRDVLRRFEEDDQTEAVVILGEVGGSL
                     EEEGAKATDLPIAAYIAGISAPPEKRMGHAGAIIEGGEGDARSKIERLRRMGVPVASR
                     PSEVPRLIKEIL"
     gene            complement(1555..2640)
                     /locus_tag="PKL26_04105"
     CDS             complement(1555..2640)
                     /locus_tag="PKL26_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA synthetase subunit beta"
                     /protein_id="HNS82912.1"
                     /translation="MKLLEHEARNIFREYGIPVPAGFLIRSPGELASRMGELSERFVL
                     KAQVEVGGRGKAGGIVVADPTTGERMAGDLFRKEIKGVPVREVLAVEYLDIQKEYYAS
                     IAIDRSTKQALILFTEEGGVDIEALAKERPEAVRRASLPLLMRDIPPFMIRDLLGAAP
                     KEIGKVLNQLYRVFSEKDALLAEINPLVTTSEGIFAADAKLIVDDNALSRQGIKLNRD
                     LTDREREAEMHGFSYVELKGDIGVIGNGAGLTMATLDLIEYYDGKAANFLDVGGGADQ
                     ERVMHAVRLVAADPSVNVIVVNLLGGITRCDEVARGIISAGISQPVIVRLAGTNEAEG
                     RQILDGSGYRMMESMEQAVKAAVEAVQ"
     gene            complement(2637..>3175)
                     /locus_tag="PKL26_04110"
     CDS             complement(2637..>3175)
                     /locus_tag="PKL26_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HNS82913.1"
                     /translation="RHEVRFSGFGGQGIILSAVILGRAAVMYDRNYAVQTQVYGPEAR
                     GGASMSAVIIDDQPILYPKVTDPDIFVIMSQEGYEKYGARAGKDAVMVLDSSLVQSRP
                     ACRFIEIPATREAKQTLGRDIVANIIMLGALVKTTHVVGEEAIEKAILDSVPKGTEAL
                     NLKAMKLGFELAEKGKTK"
BASE COUNT          559 a          986 c          901 g          729 t
ORIGIN      
        1 gcttcaggtt gcctgggctg aaccggaggg attcgggttc gtccctcttc ttggacaaca
       61 gcgggacccg ctgatgggat ggggcccggc tcaacagtgg tcttttcagg atatatgatc
      121 gtctcagaga gtgcacgggc ctctggggat atggctggca cgggctgggg cgacgcgggc
      181 cggaccgcgg tgccacccga aggacgcttt gcatgcccat gcatgataag gtgcatccac
      241 tcctctgcct gcgggagcct gtcggcaccg ttatggacaa atgtcatctt cattgtacgg
      301 agatcgtcgt ccgaagtccg tatggagagg acaaggatcg gctcctggac aggggaatgc
      361 tcaaggtagg tatctgccac ggactcgatt ggtatctcct ttgcagttac accaggggtc
      421 ttttcatcct ggtcggtgag aaagagcctt gtgttcgtca gataggcatc gaacctgaac
      481 ttcttaatct ggatatcagg ggtgtaaatc aggagctctt cgccgggcct taagtatcgc
      541 tcggggccct ggagctcctc ttctcctgtc atgggatcgt agccggtact tgggtgatcg
      601 ctcatcgttc ctccggtaga gaattataca tgtggtacta cgtgatgggt taaatggttt
      661 tcagggtccg ggagccggga tcggtgagac atcagagtat ctcctttatc aggcgcggga
      721 cttcggatgg cctcgatgcc acggggactc ccattctcct gagccgctca atcttcgagc
      781 gggcgtcccc ctcgcctcct tctatgatgg ccccggcatg acccatccgc ttctcaggtg
      841 gggcagagat tcctgcgata tatgccgcga tgggcagatc agtcgccttc gcaccctctt
      901 cttcaaggga acccccgacc tccccaagga tcaccaccgc ctctgtctgg tcatcctcct
      961 cgaagcggcg gagcacgtcg cggaaagtct ggccgatgac tgggtcacct ccgatgccga
     1021 caatggtgct ctgcccaagc cccgcccgag tcagctggtc gacaatctcg taggtgagcg
     1081 tgccgctcct tgagatgacc cctacgtgtc cccgcgagga gaggtggacc ggcatgatcc
     1141 ccatctttat ctcccctgga gagaggagcc ctgggcagtt cgggccgatc acgtcgcagc
     1201 cgtggatacg ggcaaacgag atcgcccgca tggtgtcatg taccgggatg tgctcggtga
     1261 ttgccacgac aagcgagagt ccggcgcttg ctgcctccat tatcgaatcg cccgcggcaa
     1321 agcccgggac aaagaggacg cttgcactcg cgtcgtgctc ttttagtgca tcgtgtatac
     1381 tgttgtatac cgggacgccc gcgacctcct gccctgcttt ccctggcgtc acgccggcga
     1441 ccacgccctc tcctcctacc tgccgggcat actcattcat cagcgcaatg tggaaggcac
     1501 cctgctttcc tgtcgcgccg gtgacgatca cgccggtgtg tttgtctccg tagatcactg
     1561 gaccgcctcc actgccgctt tcactgcctg ctccatgctc tccatcatgc ggtacccgct
     1621 gccatcgagg atctgtcgtc cctcagcctc gtttgtcccg gcgagcctga cgatcaccgg
     1681 ctggctgatc cctgcactga tgattccccg cgctacttcg tcgcacctcg tgatgccccc
     1741 gaggaggttg acgacgatta cgttcacgga tgggtctgcg gcaacgagcc ttacggcatg
     1801 cattacccgc tcctggtcgg caccgccgcc tacgtcgagg aagttcgctg ccttcccgtc
     1861 atagtactcg atcaggtcca gggttgccat agtgaggccg gcgccgttgc ctatcacccc
     1921 gatatcgccc ttgagctcaa cgtacgagaa cccgtgcatc tcggcctcgc gttcccgatc
     1981 ggtcaggtca cggttcagtt tgatgccctg gcgggaaagt gcattatcat caacgatgag
     2041 tttcgcatcg gcggcaaaga tgccctctga ggtcgtgacc agggggttga tctccgcaag
     2101 aagggcatcc ttctcgctga ataccctgta gagctggttt aacacctttc cgatctcttt
     2161 tggagcagcc ccgagcaggt cacggatcat gaaaggcggg atgtcgcgca tcaggagcgg
     2221 aagcgaagcc cttcgcactg cctccggcct ctcctttgcg agtgcctcga tgtccacccc
     2281 cccctcttca gtgaagagga tgagggcctg cttcgtggag cggtcgatgg cgatgcttgc
     2341 gtagtattcc ttctggatat caaggtattc cactgcaaga acctcgcgaa cagggacgcc
     2401 ctttatctct ttcctgaaga gatcccccgc catgcgctcc cctgtggtgg gatcggcgac
     2461 gacgatcccg cccgctttcc cgcgtccccc gacttccacc tgggccttga gtacgaatcg
     2521 ttcactcagt tctcccatgc gggatgcgag ttctcccggg gagcggatga gaaagcctgc
     2581 cgggacaggt atcccgtact ccctgaatat gttccttgcc tcgtgttcca acagtttcat
     2641 ttcgtcttcc ccttctccgc cagttcaaat ccaagtttca ttgctttcag gttgagggcc
     2701 tcggtccctt tcggcactga atcgaggata gccttctcga tggcctcctc tcccaccacg
     2761 tgggttgtct tcaccagcgc cccgagcatg atgatattgg cgacaatatc gcggcccagg
     2821 gtctgctttg cttcccttgt tgcagggatc tcgatgaacc tgcatgcagg cctggactgg
     2881 acgagggatg agtcaaggac catcaccgcg tcttttcccg cccgggcgcc gtacttctcg
     2941 taaccctcct gggacatgat cacaaagatg tccggatcgg tcaccttggg gtagagaatt
     3001 ggttgatcat cgatgatcac cgcgctcatg gaggcgccgc cgcgggcttc cggaccgtag
     3061 acctgggtct ggaccgcgta attcctgtcg tacatcaccg cagccctgcc gaggatcacc
     3121 gcagagagga tgatgccctg gccgccgaat cctgagaagc gcacctcgtg cctca
//
LOCUS       DAOQVJ010000147         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_35877_length_3139_cov_4.496757, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000147 DAOQVJ010000000
VERSION     DAOQVJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35877_length_3139_cov_4.496757"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..701
                     /locus_tag="PKL26_04115"
     CDS             201..701
                     /locus_tag="PKL26_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNS82914.1"
                     /translation="MPSYRTAMQLRVVGVGRVRESFLNDGIQEYRVRISPYFHVELAD
                     VREGRHPRDPSPATRAGVLKEEGERLLSAARGSGVLVLLDIRGESWSSEDLAARLSSW
                     VLEGHSTVSFLIGGPFGVSEEVKNEATHVLSLSRMTYPHQMVRLILLEQLYRAARIHR
                     GDPYHK"
     gene            complement(714..1835)
                     /locus_tag="PKL26_04120"
     CDS             complement(714..1835)
                     /locus_tag="PKL26_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HNS82915.1"
                     /translation="MFFHLILTDDCNLCCTYCRGKIFSEEAKWCGRNVLVDDLLPPDL
                     SIDLGDLYAFLRRDPDPCLTFYGGEPLLRPGLIMEIMDNAPPCRFMVQTNGILLRDLP
                     PEYLRRFETILVSLDGCAAVTDRHRGRGTYHRVMENIRYLEHTGFHGELIARMTVGDD
                     ADIAEEVKYLSRNPDHPFFSIHWQLDAEFSGDTGVRNFRKWLDESYNPGIRLLIDTWV
                     ERMAAGEGVARWYPFLQPVQDFLEGRESPLRCGAGHANYTIMTDGHIGPCPAMIGMTE
                     YYAGHISSAHPLSLPVIEVESECTKCPIRGFCGGRCLYSQIIRPWPDDMRVAVCEAVK
                     NLREGLLDALPRIRECIEMGLVLGEDFSHTRFNGCEIIP"
     gene            complement(1864..2727)
                     /locus_tag="PKL26_04125"
     CDS             complement(1864..2727)
                     /locus_tag="PKL26_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82916.1"
                     /translation="MQLLPKPGGGRFRALLLAIIAVSFILLPACADNPTEVAMTLSKR
                     ADDFVAQERYMEAIDLYNEALALDPYSSATWNRLGRAEMSVGRYPDAVAAFQKSLDLD
                     PYNSAAWRNKGDALYAQGEYGAAIDAFDRALAVNANDLYALLQKGVCLQMMGRTAQAM
                     DTYNEVIRLAEREVRRNPNEAKYDATLWTNKADALTKLGRYHEAVEAYQNAIAINPKY
                     ERAVTGIEHVNETLFRARGSPELLNTPILPVETTPGEMPVPVSILSVPFAVGILAVLS
                     CLACQRRRKCG"
     gene            2910..>3139
                     /locus_tag="PKL26_04130"
     CDS             2910..>3139
                     /locus_tag="PKL26_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82917.1"
                     /translation="MDHPTNTGNETRPVPTIHLDAAEMAILLLLGRRPATLADLAHST
                     WLDPSQLYLVLGRLVHRWLAEQSGAHYGLTRSG"
BASE COUNT          649 a          932 c          880 g          678 t
ORIGIN      
        1 ggaagaggga acccgaaaag agcaagggga gttgccgggt tcctctgata tcattgggtt
       61 tgagggctgc agagggaggc tcactctcgc ctccacacat cttcatcttt tcatgggagc
      121 atataagagg cagccgtggg gggtgaaagt gaacctttcg gacctgtccc ctggcaggat
      181 tgcgtctcct ttaaaatccc gtgccgtcct accgtaccgc gatgcagttg cgggtcgtgg
      241 gggtagggag agtgagggag tctttcctca atgacgggat acaggagtat cgtgtgagaa
      301 tctctccgta cttccatgtc gagcttgccg atgtgcgcga gggcaggcat cctcgcgatc
      361 cctcccctgc cacacgtgcc ggggtgctca aagaggaagg ggaaaggctg ctctcggctg
      421 cccggggaag cggggttctg gtgctcctag atatccgcgg cgagtcctgg agcagtgagg
      481 acctcgcagc ccggctctcc tcttgggtcc tcgaaggcca ctccaccgtc tcgtttctca
      541 tcggcggccc cttcggagtg tcagaggagg tcaaaaacga ggccacgcat gttctctccc
      601 tctcccgcat gacctatccc caccagatgg tccggctcat ccttctcgaa caactgtatc
      661 gcgcggccag gatccaccgc ggcgatcctt accataaata ggcaaaaggg ggatcagggg
      721 atgatctcgc acccgttgaa gcgcgtatgg gagaagtcct ccccaagaac caggcccatt
      781 tcgatgcatt cccttatgcg gggcagtgca tccagcagcc cttcccgcag gttcttcaca
      841 gcctcgcaaa ccgcgaccct catatcatca ggccatggcc tgatgatttg ggaataaagg
      901 caccttcccc cgcagaaccc ccgtatcggg cacttcgtgc actcgctctc cacttcgatg
      961 acagggagag agaggggatg tgcacttgaa atatgccctg cgtaatactc tgtcatcccg
     1021 atcatcgccg ggcagggccc gatatgccca tcggtcatga tggtgtagtt cgcgtgcccg
     1081 gcaccgcagc gaagcgggct ctccctccct tccaggaagt cctggaccgg ctggaggaac
     1141 gggtaccacc gggcaacccc ttcccctgcc gccatcctct ccacccatgt atcgatcagc
     1201 aggcgtatgc cagggttgta ggactcatcg agccattttc ggaagtttcg cacccccgtg
     1261 tcaccggaga actccgcgtc aagctgccag tgaatggaaa agaatgggtg gtccgggttt
     1321 ctcgagagat acttgacctc ctctgcgatg tctgcatcgt ccccgactgt catcctggcg
     1381 atcagttcac cgtgaaaacc ggtgtgctca aggtacctga tgttctccat cacccggtgg
     1441 taggtcccac gaccacggtg cctgtcggtg acggcggcac agccgtcaag agagacgagt
     1501 attgtctcaa aacgcctgag gtattccggc ggcaggtcac ggagaagaat cccgttcgtc
     1561 tggaccatga acctgcacgg gggtgcattg tccatgatct ccatgataag gcctggccga
     1621 agcaggggtt ccccgccata aaaagtgagg cagggatccg ggtccctgcg aagaaatgca
     1681 tacagatccc caaggtcgat gctgaggtca ggtgggagca ggtcgtctac caggacgttc
     1741 cttccacacc actttgcctc ctctgagaag atcttgcccc ggcagtaggt gcagcaaaga
     1801 ttgcagtcat cggtcagaat gagatggaag aacatggctg gcgcgtggtg agggattata
     1861 cgatcaaccg catttccggc ggcgctggca ggcaaggcag gataatacgg ccagaatacc
     1921 gacggcaaac ggcaccgaca gaatagagac agggaccggc atctcccccg gcgttgtttc
     1981 gacgggcaga atgggtgtgt tgagaagttc cggtgaaccc cgtgccctga acagcgtctc
     2041 gttcacgtgc tcaattcccg tgaccgcacg ctcgtacttg ggattgatcg cgatggcgtt
     2101 ctggtatgcc tcgactgcct catggtagcg gccgagcttt gtgagggcgt cagccttgtt
     2161 ggtccagagg gtggcatcgt acttcgcctc attcgggttc cgcctcacct ctctctctgc
     2221 aagccggatc acctcgttat aggtatccat tgcctgggca gtcctgccca tcatctgaag
     2281 gcagaccccc ttctggagca gggcgtacag gtcattggca ttgacggcaa gcgcgcgatc
     2341 aaaggcatca atcgcagccc catactctcc ctgcgcatac agggcatctc ccttgttcct
     2401 ccatgccgct gagttatacg gatcgagatc aagcgacttc tggaacgctg cgaccgcatc
     2461 ggggtaccgc cccacgctca tctctgctct cccaagcctg ttccaggtcg cgctgctgta
     2521 gggatcgagg gcaagggcct cattatagag atcgatcgcc tccatgtacc tctcctgggc
     2581 tacgaagtca tcagccctct tggagagggt catcgccact tcggtgggat tgtcggcaca
     2641 tgccgggagg agaatgaagg ataccgcaat tattgcaagc agcagtgcac ggaacctgcc
     2701 accacccggt ttggggagga gttgcatacc cggtgatata ttgcctccat gcgcttaata
     2761 tgataacgaa tacacaaaaa aaccctttaa tacttttctg gtctcaccaa tctcatgcat
     2821 cgtattccat tctacgtatt ggataatgca atgtgatgca ccgacttcac cattgtcgtc
     2881 tttccccaag agttatccct gcacacccta tggaccaccc gacgaataca gggaatgaaa
     2941 cccgccctgt ccccacaata caccttgacg cggcagagat ggccattttg ttgttacttg
     3001 gccggcgccc tgcgacgctt gccgatcttg cacattcgac atggctcgat ccgtcgcagc
     3061 tctaccttgt cctcgggcgt ctcgtgcacc ggtggcttgc agagcaatcg ggagcacatt
     3121 acggccttac caggtctgg
//
LOCUS       DAOQVJ010000148         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_36058_length_3127_cov_5.231120, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000148 DAOQVJ010000000
VERSION     DAOQVJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36058_length_3127_cov_5.231120"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1244
                     /locus_tag="PKL26_04135"
     CDS             48..1244
                     /locus_tag="PKL26_04135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNS82918.1"
                     /translation="MTVIRSIQLRTILDSRGNPTVEADIFTDSGFGRSAAPSGASTGL
                     HEAKVKDPGEAVPFAISNLVPGLIGVDSRDQVAFDSILREEDGTSDFSKIGANVAVAL
                     SLANAKAAASSLGMEVYSYLGGVFVPEMPLPLGNIIGGGAHAKGATDIQEFLVVATGA
                     GGPTEAVFANARVHRRVKDILQASGKACGKGDEGAWAPQIGDHEAFEIIRQATGEVSD
                     ELSLSISMGIDVAASQMWDADSGCYIDRSGRRTTEDYIAYIAELVDRYELVYVEDPLY
                     EEDFSGFADLTEQVGDRCLVCGDDLFVTNAARIAEGIEAGSANCALIKPNQVGTLTDT
                     FEAVHLSHLHGMDTVMSHRSGETTDATIAHLATAFGCVFLKTGAVGGERIAKLNELIR
                     IEEQIA"
     gene            1241..1849
                     /gene="rpsB"
                     /locus_tag="PKL26_04140"
     CDS             1241..1849
                     /gene="rpsB"
                     /locus_tag="PKL26_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNS82919.1"
                     /translation="MTETEMEIELKEPLVPVEEYLAAGVHIGTQQKSKDMMKFIYRVR
                     GDGLYILDIRETDERIKTSAKFLSQFDPTSILVVTSRQYGQYPAKKFAESIGGMAVTG
                     RFIPGMLTNQNLANYIEPQVLVVTDPIGDAQAVKEAVQSGIPILALCDTNNMTSLVDL
                     VIPTNNKGRKALSMIYFLLTREMLRLRGITTSLTPEDFETEL"
     gene            1879..2670
                     /gene="amrB"
                     /locus_tag="PKL26_04145"
     CDS             1879..2670
                     /gene="amrB"
                     /locus_tag="PKL26_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNS82920.1"
                     /translation="MKTRRCGVAGMFYPRDPHHLEQLLEKFFSNKNAGVDALGIVSPH
                     AGYPYSGEVSALAYTAFPASFAGTFVVIGPSHRGYRTCASLIPWETPLGIVDNDTVFG
                     SSLDLDPDEVSHQEQENSLEVQIPFIKYRFPRARIVPILMGDQTLEGARHLSRTLIDG
                     IRRSGRNDIRIVASSDFSHYVPDAVARENDLYAIAALEHLDVEEFYRRIQSRRVSTCG
                     YGPVATMCLVCRELGASSGRLLRYATSGDVTGDPEVVGYAAIAVM"
     gene            2672..>3127
                     /locus_tag="PKL26_04150"
     CDS             2672..>3127
                     /locus_tag="PKL26_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNS82921.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGVAMAIRPRVFVTVRKSKSAH
                     HARSPYIDSCFDEMGVKGSVYVNSQLPSSSGLGSSAAVTVATLSAINDEFGKKYTMEE
                     IANLAFAIEKKVQKGRASPTDTSVSTFGGIVLITGNQRRRLPPQNLPLVV"
BASE COUNT          690 a          884 c          951 g          602 t
ORIGIN      
        1 tccagcacaa cgtcatacct atcacggtga agaggaagta gtcagccatg acggttattc
       61 gcagcattca actgcgcact atactggaca gcaggggcaa ccccacggtg gaggcggata
      121 tcttcaccga ttcgggtttc gggagatccg cagcccccag cggagccagc acaggccttc
      181 acgaggcgaa agtgaaggac cccggagagg cagtcccgtt cgcaatctca aatctcgtcc
      241 caggacttat tggagttgac tcccgggacc aggtcgcatt cgattccatc cttcgggagg
      301 aggacggaac atccgatttc tcgaagatag gggcaaatgt cgcggtcgca ttgtcccttg
      361 cgaatgcaaa ggcggctgca tcatccctcg ggatggaggt atactcctat ctcggcgggg
      421 tgttcgtgcc cgagatgccg ctcccgctcg gcaacatcat cggcggcggt gcccacgcga
      481 agggcgcgac cgatatccag gaattcctgg tggttgccac cggtgcggga ggtcccactg
      541 aggcggtatt tgccaatgcc cgggttcaca ggagggtgaa ggatatcctc caggcatccg
      601 gaaaggcctg tggaaaaggc gacgaagggg catgggcccc gcagatcggc gaccatgagg
      661 cctttgagat cattcgccag gcgactgggg aggtgtcaga cgagctctct ctctcgatct
      721 cgatggggat tgatgttgcc gcaagccaga tgtgggacgc tgactccggg tgctacatcg
      781 acagaagcgg ccgtcgcaca accgaggact acatcgccta catcgccgag cttgtggaca
      841 ggtatgaact ggtctacgtc gaggacccgc tctacgagga ggacttctcg ggttttgctg
      901 acctcacaga gcaggtcggt gaccggtgcc tggtctgcgg agatgacctc tttgtcacca
      961 acgccgcccg gattgcagaa ggcatcgagg caggatctgc gaactgcgca ctgatcaagc
     1021 cgaaccaggt tggaaccctc actgatacct tcgaggcagt ccacctctcc caccttcatg
     1081 gaatggacac ggtgatgagc caccgatccg gggagacgac ggacgctacc atcgctcacc
     1141 ttgcgacggc attcggctgc gtattcctga agaccggtgc agtcggagga gagcgtatcg
     1201 ccaagctgaa tgaactgata cgaatcgagg aacagatagc atgacagaga cagagatgga
     1261 aattgagctg aaagagccgc tggttcccgt ggaagagtac cttgccgcgg gcgtccatat
     1321 cggcacccag cagaagagca aggacatgat gaagttcatc taccgcgtga ggggagatgg
     1381 attgtatatt ctggacatca gggagacgga cgagcggatc aagacttccg caaaattcct
     1441 ctcccagttc gacccgacca gtatccttgt ggtcacatca aggcagtacg ggcagtatcc
     1501 tgcaaagaaa tttgcagaat ccatcggggg catggcggtc accggccggt tcatcccggg
     1561 gatgcttacc aaccagaacc ttgccaacta catcgagccg caggtcctgg ttgtcaccga
     1621 tcctatcggt gacgcgcagg cggtaaaaga ggcagtccag agcgggattc ccatccttgc
     1681 gctctgcgat accaacaaca tgaccagcct cgtggatctc gtgataccta ccaacaacaa
     1741 ggggagaaag gcactctcga tgatctactt cctgctcacc cgcgagatgc tccgcctgag
     1801 ggggatcacc acttccctca ccccggaaga tttcgagacc gaactctaat ataggtgaga
     1861 tacgagaggg tagatccgat gaagactcgg agatgcgggg ttgccggcat gttctaccca
     1921 agggaccctc accacctcga gcagctgctg gagaagttct tctcgaacaa gaacgcgggg
     1981 gtggatgcgc ttggaatcgt ctccccccat gcagggtatc cgtattccgg cgaggtgtct
     2041 gcactcgcat atactgcctt ccccgcatcg tttgcaggaa cgttcgtggt gatagggccg
     2101 agtcacaggg ggtatcgcac ctgcgcctcg ctcatcccat gggagacccc gcttggaatc
     2161 gtcgacaatg acacggtctt cgggtcatcg cttgacctcg accctgacga ggtctcgcac
     2221 caggagcagg agaactccct tgaagtgcag atcccgttca tcaagtaccg gttcccgcgg
     2281 gcaaggatcg ttcccatcct gatgggtgac cagactctcg agggggcccg ccatctctcc
     2341 aggacactca tcgacgggat taggaggagc ggacgcaacg atatcaggat cgtggcatcg
     2401 agcgatttct cccattatgt cccggacgcg gtggcaaggg agaacgacct ctatgccatc
     2461 gccgcactcg agcacctgga cgtggaggaa ttctaccgcc gcatccagtc ccggagggtg
     2521 agcacatgcg ggtacggccc ggtcgcaacc atgtgcctgg tatgcaggga acttggagcc
     2581 tccagcggcc ggctgttgcg gtatgccacc agcggcgatg tgacgggtga ccccgaagtg
     2641 gtcgggtatg cagccattgc ggtgatgtag ggtggcgacc tggagcgccc caggcaaggt
     2701 cttcctgttt ggggagcacg cggtggtgta cgggaagccc ggtgtggcaa tggccatacg
     2761 cccgagggtc tttgtgaccg tgaggaagag caagtctgcc catcatgcga ggtcgcccta
     2821 tatcgacagc tgctttgatg agatgggggt gaaagggagc gtttacgtga actcccagct
     2881 cccgtcttcg tcgggtcttg gatcctctgc agcggtgact gtcgcaacac tctcggccat
     2941 caacgatgaa ttcgggaaga agtataccat ggaagagatc gccaacctgg catttgccat
     3001 cgagaagaaa gtgcagaaag gcagggcaag ccctaccgat acaagtgtat ccactttcgg
     3061 tggcattgtc ctgataaccg ggaaccagcg ccggcggctg cccccccaga acttacccct
     3121 ggtggtg
//
LOCUS       DAOQVJ010000149         3082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_36853_length_3082_cov_3.584077, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000149 DAOQVJ010000000
VERSION     DAOQVJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36853_length_3082_cov_3.584077"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(336..983)
                     /locus_tag="PKL26_04155"
     CDS             complement(336..983)
                     /locus_tag="PKL26_04155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNS82922.1"
                     /translation="MEQINPTRMELIKKNAQIKLAEQGRDLLREKMDALIQEFFNVME
                     SVSQSRSELEKLSEVAQHALLIAEAIDDPVTLKSASFATRRGITLDIKGKNVMGVPVP
                     IIEKKRFVKSLFERGYSILGVSGRIDEAAEKYEAELDLIITLAETETSLRRLGSEIQM
                     TRRRVNALEQVLIPELKRQAKYIEITIDERDREDLYRLKKVKKIIERKKRETIDT"
     gene            complement(983..2383)
                     /locus_tag="PKL26_04160"
     CDS             complement(983..2383)
                     /locus_tag="PKL26_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNS82923.1"
                     /translation="MIRVPLFTKEARAINYVSGPLIFIQNVKGVSYGEIVRIILPTGE
                     ERTGQVLDISKDIAVTQVYEGTSGIDNKETRVRFTGEPPKVKVSIEMLGRIFNGIGKP
                     RDGGPEIIEESTLDINGMPINPSARDKPADFIQTGMSTIDGLNTLVRGQKLPIFSGAG
                     LPANKLAAQIARQARVLGEEEKFAVIFVAMGITYKEASFFMRDFEKTGALERVVFFMN
                     LADDPTIERIATPRCALTAAEFLAYEHDLHVLVILTDMINYCEALREISTAREEVPGR
                     RGYPGYMYTDLASIYERAGRVKGRKGSITQIPILTMPDDDITHPVPDLTGYITEGQIV
                     LSRDLFRRGADPPIDPLPCLSRLMNLGIGPGKTREDHRNVADQLYASYAYGRDLRRLV
                     AIVGEEALTELDKRYLKFADEFERHFITQGDENRSIERTLEIAWQLFTLLPEEELKRI
                     KREFIQKYHPAAQKGM"
     gene            complement(2380..>3082)
                     /locus_tag="PKL26_04165"
     CDS             complement(2380..>3082)
                     /locus_tag="PKL26_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNS82924.1"
                     /translation="EGYPAYLATRLAAFYERAGRVVCLGSDERKGSVTVVGAVSPPGG
                     DFSEPITQNTLRIAGTFWALDTSLAYRRHFPSVNWIKSYSLYLDQIQDWYTASGFSDW
                     KELRARAMYLLQKEVELQEIVQLVGPDALPESEKAILEITRMIREDFLQQSAYHDIDS
                     FCSLEKQYWMLKVILFFYDRLNQSMGKGIPLSKILKLPVKAEIGRMKELTEIEKIKDL
                     VDQIDGSLGSLEVER"
BASE COUNT          651 a          831 c          797 g          803 t
ORIGIN      
        1 gacgaaaccg tgttggtggt ggaagttacc taatcaccta gattaataac ccgggcctta
       61 attgtgtaat gctgttcatt cactacatat tttccttctt atctcttccc tgcccgcgtt
      121 atgttgtcgc aatccgtgaa ggtgatgtcc caggttgtga tgtgggccat gaggaaaaaa
      181 ggtcgtcctc ctgaaacctg gacgaaccaa tctctcacaa agacaaaata ccgctatgga
      241 tcctgatgtg gatttccgaa cggaaaaacc gtaatcctgc tggattaaag agtcccatag
      301 cgacgaggga gatgattcat atgggtacca tgccttcagg tatctattgt ctctcgtttc
      361 tttctctcga tgatcttttt tacttttttc aaccggtaaa ggtcttcgcg atccctttca
      421 tcgatagtga tttcgatgta ttttgcctgt cgcttcagtt ctggaatgag tacctgttca
      481 agtgcattta cgcgccgtcg cgtcatctgg atctcgctgc cgagccttcg gagagatgtt
      541 tcagtctctg cgagagtgat gatgagatcc agctccgctt cgtatttctc tgccgcttcg
      601 tcaatccgtc cgctgacccc caggatgctg tacccccgtt cgaagaggct cttcacaaaa
      661 cgcttctttt cgatgatagg aacaggcaca cccatgacat tctttccctt gatatcaagg
      721 gtgatacctc tcctggtggc aaatgaggca gacttcaatg tcacggggtc gtctattgcc
      781 tctgcaatga gcagggcatg ctgagcaacc tcggagagct tttcaagctc gcttcgcgac
      841 tgcgataccg actccatgac attgaaaaat tcctggatga gcgcatccat cttttcacgg
      901 agaagatcgc gcccctgctc tgcaagcttg atctgtgcgt tcttcttgat gagttccatc
      961 cgggtcgggt taatctgttc catcacatcc ccttttgagc agcaggatga tatttctgaa
     1021 tgaattcacg tttaatccgt ttcaattcct cttcagggag cagggtgaac aactgccagg
     1081 ctatctcaag cgtcctctcg atgctccggt tctcgtctcc ctgcgtgatg aaatggcgct
     1141 cgaactcgtc cgcgaatttc aggtatctct tgtcaagctc ggtgagcgcc tcttccccca
     1201 caatcgcgac gagcctccgc agatccctcc cataggcata tgatgcatag agctgatccg
     1261 cgacattccg gtgatcctct ctcgtctttc caggtccgat accaagattc atcaggcgtg
     1321 agaggcatgg gagtgggtcg attggaggat cggccccgcg gcgaaagaga tcgcgtgaga
     1381 ggacgatctg tccctcggta atatagccgg taaggtcagg gaccgggtgg gtgatatcgt
     1441 catccggcat cgtgaggatt gggatctggg tgatggatcc tttccggcct ttcaccctcc
     1501 ctgcccgctc gtagatggat gcgaggtcgg tgtacatata cccgggatac cctcttcggc
     1561 ctggcacctc ctcccgtgcc gtggagatct cgcgcagtgc ctcgcagtag ttgatcatgt
     1621 cagtgaggat aacaagcaca tgaagatcgt gctcataggc gagaaattca gcagcagtga
     1681 gggcacaacg tggggtggcg atcctctcta tagtggggtc gtcagcaagg ttcatgaaga
     1741 acaccaccct ctctaatgca cctgtctttt caaaatccct catgaaaaag gaggcctcct
     1801 tgtatgtgat gcccatggcg acaaagatga ccgcgaactt ctcttcttcc ccgaggaccc
     1861 tggcctggcg tgcaatctga gcggcaagct tgttcgcggg aagacccgca cccgaaaaga
     1921 tgggcagttt ctggccgcgg acaagcgtat tgaggccatc aatcgttgac atgccggtct
     1981 ggatgaaatc agcgggtttg tctctggcgg atggattgat gggcataccg ttgatgtcca
     2041 gggttgattc ttcaatgatc tcgggtcctc cgtcgcgggg ttttcctatg ccattgaaga
     2101 tccgtcccag catctcgatt gagactttta ccttcggggg ttctccggtg aaccggaccc
     2161 gtgtctcctt gttgtctata ccgctggtcc cttcgtatac ctgggtgacg gcgatatcct
     2221 tcgatatatc aaggacctgc ccggtgcgtt cttcgcctgt ggggaggatg atccggacga
     2281 tctcgccgta cgagacgcct ttcacatttt gaataaaaat cagagggcct gaaacatagt
     2341 tgattgcccg ggcctctttt gtaaagagcg gcacccggat catcgctcca cctccagaga
     2401 accaagagag ccgtcgatct gatcgacaag gtccttgatc ttttcgattt cggtgagctc
     2461 cttcattcgc ccaatctctg ctttcacggg cagtttcagg attttgctga gaggaatccc
     2521 ctttcccatg gactggttca accggtcata aaagaagagg atcaccttga gcatccagta
     2581 ttgcttctca agcgagcaga acgaatcgat atcgtggtat gcactctgct ggagaaagtc
     2641 ctcgcggatc atgcgtgtga tctcaagaat tgccttctcg ctctccggta atgcatctgg
     2701 gcccacgagc tgcacgatct cctgcagttc gacttctttc tggaggagat acattgcccg
     2761 cgccctgagc tccttccagt cggaaaaccc actcgcggta taccaatcct ggatctggtc
     2821 caggtaaagg gaatagctct ttatccagtt cacggagggg aagtgccgcc tgtacgcaag
     2881 gctcgtgtcg agtgcccaga atgttccggc aatgcgaagg gtgttctggg ttattggttc
     2941 ggagaaatct ccgccgggag gcgaaacggc cccgaccacc gtgaccgaac ccttccgttc
     3001 gtcagagccg agacagacca cgcgacccgc cctctcgtag aatgcagcga gccttgtcgc
     3061 aaggtatgca gggtaccctt ct
//
LOCUS       DAOQVJ010000150         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_36922_length_3078_cov_4.254383, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000150 DAOQVJ010000000
VERSION     DAOQVJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36922_length_3078_cov_4.254383"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKL26_04170"
     CDS             <1..920
                     /locus_tag="PKL26_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS82925.1"
                     /translation="AGDEIVPGTPADEVLAPLPYEVQRSLYSVLRTGKVVTARISWIE
                     REGGPLVLESTAFPVPGTGGGMPELAAWTAAVTPGELPAGEALAMEASRSKGKELLSV
                     VRHDILNQLTILIGFLQFSEDFIEDSRVREFLAKEEAAGHVIQALIEFTRDFQDLAVE
                     DPRWMRLDAVIRSAQGRVDTKGIEIVADAGTTEVYASPLIRHVFLTFIKNAVDHAHGL
                     TRITLSQGVDGGDLVIACEDDGPGIPESERKTLFDRGHGRNRGYGLWLAKEILSITGA
                     KIREAGKEGGGARFEIRVPAGMWREGDAS"
     gene            complement(921..2588)
                     /locus_tag="PKL26_04175"
     CDS             complement(921..2588)
                     /locus_tag="PKL26_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82926.1"
                     /translation="MITVLLVHDNSDALESTRAYLERMGEIRVDAVHSTKQALEMARR
                     RRYDIIVSYYRLPEVNGIEFLADMTGVELLKELKGTHAGIPFILYHRTDRDSLLIEDV
                     HQAAEVHGQREGSRGPVQEMRDILYQAVLRKKAERDQVLRCDMLSAILSVSPLWICQV
                     RGGNFEWVNSTMARSLGMDAGALAGQGVQAVFPGNEEYERAFRELCHRSDDQGWGHAD
                     TVLKRADGTLIPCRLSIRAMDPADPSRGQVIVCEDRSEHAKLIDAQKESDLRYREFLQ
                     NASSLIIKLDPEGVVTFFNRHAQAFFGYSEAEVLGKNIRDTLVPRKDGKASPGFVTDT
                     GANHEGGSLRIYEMQLRGGEPAWVAWHTRAIRNADGHLVEVVCIGHDITDHEHRDRRR
                     ISTAMWRDTVITGTDVKEEVFDSVLHICIEIAKEGREGKQVGTAFVIGDSDAVLVKSK
                     QLILNPFEGHPATDRMITNHDIKENIKELAQLDGAFVVRGDGRIEAAARYITIDTSKV
                     GIQKGLGTRHSSVAGITLVTRAIGIVLSQSGGKISIFKDGRMVQEIA"
     gene            complement(2716..>3078)
                     /locus_tag="PKL26_04180"
     CDS             complement(2716..>3078)
                     /locus_tag="PKL26_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNS82927.1"
                     /translation="QDPEYYIHRIGRTGRAGKSGKAFTFVSGKEMWKLRDIQKFAKIR
                     IARHAVPADEDLRERRIVNMIGKIRENITAGDLEPYIARVEQVMGDEHTSLEVAAALF
                     RMQSGADAGVSRGKGRGK"
BASE COUNT          502 a          965 c         1003 g          608 t
ORIGIN      
        1 ccgcgggaga tgagatagtc cccggcaccc ctgcagacga ggtgctcgcg ccgctcccct
       61 acgaggtcca gcggtcgctg tattcggtgc tgcggacggg aaaggtggtg actgcccgaa
      121 tctcgtggat tgagagggag ggcggtcccc tggtcctcga gagcacggcg tttccggtcc
      181 ccggcaccgg gggagggatg cccgaacttg cggcgtggac tgccgcagtg acgcctggcg
      241 aactgccggc cggggaggcc ctcgcgatgg aggcctcgcg gagcaaggga aaggaactcc
      301 tttcggtggt caggcacgat atcctcaacc agctgaccat cctgatcggc tttctccagt
      361 tttccgagga cttcatcgag gactccaggg tcagggagtt cctcgcgaaa gaggaggccg
      421 cgggccatgt catccaggcg ctgatcgagt tcacccgcga cttccaggac ctggcagtcg
      481 aggacccgcg ctggatgcgg ctcgatgccg tcatccggtc cgcccagggg agggtcgata
      541 cgaagggcat cgaaatcgtc gcggatgccg gcacaaccga ggtctatgcg agcccgctga
      601 tccggcatgt ctttttgacg ttcatcaaga atgcggttga ccatgcgcac gggctcaccc
      661 ggatcaccct ctcgcaggga gtggacggcg gcgacctcgt tattgcctgc gaggacgacg
      721 gtcccggcat cccggagtca gagagaaaga cgctctttga ccggggccac ggcaggaaca
      781 ggggatacgg cctctggctc gcaaaggaga tcctctcgat cacgggggca aagatccggg
      841 aggccggaaa ggagggcggc ggggccaggt tcgagatacg ggttcccgcc gggatgtggc
      901 gggaagggga cgcctcctga tcaggcgatc tcctgcacca tccgtccgtc cttgaagatc
      961 gagatcttgc ccccgctctg cgagaggacg atgccgattg ccctcgtgac cagggtgatc
     1021 ccggcaacag acgagtgccg cgtcccaagg cctttctgga tccccacctt gctcgtgtcg
     1081 atcgtgatgt aacgggcggc agcttctatc cgcccgtcgc cccggaccac gaatgcaccg
     1141 tcgagctgcg ccagctcctt tatgttctcc ttgatatcgt ggttggtgat catccggtca
     1201 gtggcagggt ggccctcgaa cgggttgagg atcagctgct ttgacttgac cagcaccgcg
     1261 tccgagtccc cgatgacaaa cgcggtcccc acctgcttgc cctctctccc ctcctttgcg
     1321 atctcgatgc agatgtggag gaccgagtca aagacctctt ctttgacatc ggtcccggtg
     1381 atcacggtgt ccctccacat agccgtgctg attctgcgcc ggtcccggtg ctcgtggtcg
     1441 gtgatgtcgt ggccgatgca gaccacctcc acgaggtgcc cgtccgcgtt gcggatggcc
     1501 ctcgtgtgcc aggcgaccca ggcgggctcc ccgccccgga gctgcatctc gtagatgcgg
     1561 aggcttccgc cctcgtggtt tgcgcccgtg tcggtgacaa agcccgggga ggccttccca
     1621 tccttccggg ggaccagggt gtcgcggatg ttctttccga ggacctctgc ctccgagtag
     1681 ccgaagaagg cctgggcgtg cctgttgaag aaggtgacga ccccttcggg gtcgagcttg
     1741 atgatcaggc tcgaagcgtt ctggaggaac tcgcggtacc ggaggtcgct ctccttctgc
     1801 gcatcgatga gttttgcatg ctctgaccgg tcctcgcaga ctatcacctg cccccgcgag
     1861 gggtccgccg ggtccatcgc ccggatgctc aggcggcagg ggatgagcgt cccgtccgct
     1921 cttttcaaca cggtatccgc gtggccccat ccctggtcgt cggaccggtg gcaaagctcc
     1981 ctgaaggcac gctcgtactc ctcgttcccg gggaagactg cctggacgcc ctgcccggcg
     2041 agcgcccccg cgtccatgcc gaggctgcgg gccattgtcg aattgaccca ttcgaagttc
     2101 ccgccccgca cctggcagat ccagagcggc gagacagaga ggatagccga gagcatgtcg
     2161 cagcggagga cctggtcacg ctcggctttc ttccggagca ccgcctggta gaggatgtcc
     2221 cgcatctcct gcacaggacc ccgcgagccc tccctctggc cgtgtacctc ggctgcctgg
     2281 tgcacgtcct cgatgaggag gctgtcccgg tcggtccggt ggtagaggat gaacgggatg
     2341 cctgcgtggg tccctttcag ttctttgagg agctcgactc cggtcatgtc ggccaggaac
     2401 tcgatcccgt tcacctcggg gaggcggtag tacgagacga tgatgtcgta tctcctccgg
     2461 cgggccatct ccagggcctg cttcgtggag tggacggcgt cgacccggat ctcccccatc
     2521 ctctcgaggt atgccctggt gctctcgagg gcgtcgctgt tgtcgtggac gagaagaacg
     2581 gtgatcatgg ctgacggctg ctccgggaga tcgttcctgg gagtatggga aaaggtagga
     2641 gatgccagaa tttaataatt attggtgcga aatccgggat tgagcgcgca ggcggcggaa
     2701 tttaaagaaa tggtcttact tcccccttcc ttttcccctg ctgacgccgg catctgcgcc
     2761 ggactgcatc cggaagagcg ctgcggcgac ctcgagcgac gtgtgctcgt cccccatcac
     2821 ctgctcgacc ctcgcgatgt acggctccag gtcgccggcg gtgatgttct cacggatctt
     2881 gccgatcatg ttgacgatgc gccgctcgcg gaggtcctca tcggccggga cggcgtgacg
     2941 ggcgatgcgg atcttcgcaa acttctggat gtcccggagt ttccacatct ctttcccgga
     3001 gacgaaggtg aaggccttgc cggacttgcc ggccctgccg gtccgcccga tgcggtgaat
     3061 gtaatactcg gggtcctg
//
LOCUS       DAOQVJ010000151         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_36929_length_3078_cov_3.345683, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000151 DAOQVJ010000000
VERSION     DAOQVJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36929_length_3078_cov_3.345683"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKL26_04185"
     CDS             complement(<1..753)
                     /locus_tag="PKL26_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS82928.1"
                     /translation="MYAERMEMLPPYLFARIDEMKAKKIQAGVDVIDLGVGDPDLPTP
                     PFVVEELCRAARDPAHHHYPSYAGMTDYRRAVAGWYTRRFGVELDPSTEVLALMGSKD
                     GIAHVPEAFVNPGDYVLAPSPGYPVYRTSTLFAEGKVYEMPLLAKNHFIPDLDDIPNK
                     VADAAKIMFLNYPNNPTSAVTLPGFFESAVEFAREHEIVIIHDNAYSEISFDGYRAPS
                     FLETEGAMDVAIEMHSLSKTFNMTGWRIGMAVG"
     gene            961..1290
                     /gene="cutA"
                     /locus_tag="PKL26_04190"
     CDS             961..1290
                     /gene="cutA"
                     /locus_tag="PKL26_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNS82929.1"
                     /translation="MNEKEQSRINVILVTSGRNEGPAIARHLVEKRMAACVNIAEIRS
                     CYRWEGRFCDDPEVLLIIKTAHEKVEEALAAIREVHPYTLPEMIVLPVVAGYPPYLAW
                     VEEETTR"
     gene            1287..2048
                     /gene="fdhD"
                     /locus_tag="PKL26_04195"
     CDS             1287..2048
                     /gene="fdhD"
                     /locus_tag="PKL26_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNS82930.1"
                     /translation="MNTVSISGIQVRNGRPGEIEDTVVRESRFSLYLNGTYFTSMVAS
                     NDQLDELGAGFVTCQGISGRVDEVRVKGEEIHVIAPITGELLQEIISTGALGVRRPFL
                     PVTSSLTLTTDDVYRITGEIETETWRRTGGVHCSVLFHENELLVKASDLGRHSTVDKV
                     VGHAVIRGIDRSRCVLGCTGRQPRDMVTKAAHAGIPVVVSRAASTEQGIETARKAGIT
                     LICFSRGDRFTVYTHPEKVRDLRGYLNQAGVRNGK"
     gene            2135..2572
                     /locus_tag="PKL26_04200"
     CDS             2135..2572
                     /locus_tag="PKL26_04200"
                     /inference="COORDINATES: protein motif:HMM:NF020570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HNS82931.1"
                     /translation="MGCPQVPVQSPVALYLMQRLRMLGISPVVAGNQAARMQVEVSDP
                     LHHYSGEMVDLDTCIADLAEGRRSYSYYMIFVHNDAGVSYAATVQAITGKKVVAILYG
                     EHFREVGETIGFPCEKVAAKAVHNPMPLKKKIDEVLPWVVSNL"
     gene            2551..2829
                     /locus_tag="PKL26_04205"
     CDS             2551..2829
                     /locus_tag="PKL26_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HNS82932.1"
                     /translation="MGCIESLKYEIVLRGASFAECRGYVEEHCSERVIVDPGFKIFEK
                     PIIGVPPVVIGLDGDVITFPYTKPCYGTFLLKTENPPEAARIRSLGKK"
BASE COUNT          695 a          796 c          914 g          673 t
ORIGIN      
        1 tccaaccgcc atcccgatcc gccaccctgt catgttgaag gtcttcgaga gggaatgcat
       61 ctctattgca acgtccatcg cgccttccgt ctcgagaaac gagggagccc ggtagccgtc
      121 gaaggagatc tccgagtagg cgttgtcatg gatgatgact atctcgtgtt cgcgtgcaaa
      181 ttcaaccgcg ctctcaaaga atcccgggag ggtcaccgca gatgtgggat tgttcgggta
      241 gttgaggaac atgatctttg cggcatctgc aaccttgttg gggatgtcat cgagatcagg
      301 gatgaagtgg ttcttggcca gaagtggcat ctcgtagact tttccctcgg caaagagggt
      361 ggatgtccgg tataccgggt aacccgggct cggtgcgagg acataatctc ccgggtttac
      421 gaatgcctcc ggtacatgtg cgatcccgtc ctttgagccc atcagggcaa gcacttcagt
      481 ggaagggtcc agttccacgc cgaaacgacg tgtgtaccat cccgcgacag cgcggcggta
      541 atctgtcatc cctgcatagg acgggtaatg gtggtgagcc gggtcccttg ccgcccggca
      601 gagctcttcc acgacgaaag ggggggtggg caggtccggg tcacccacgc ccaggtcaat
      661 gacatcgact cccgcctgta ttttctttgc cttcatctcg tctatcctgg cgaacaggta
      721 gggagggagc atctccattc gttccgcgta catgtgcagg agtatagggc ctgctcattc
      781 attaatgatc accacgggct gtcgtccaaa ataggcgctg atgcgttcag atcctgaaat
      841 atcccattaa ttgagagaat tgaagcaata ctcttatatc ccgcgcccgg gtagtaaaga
      901 gcatgcacat tccccggccg gtttcacggg tgtgtcccgg atcatgaagg tgaacaggag
      961 atgaatgaaa aggagcagtc acgaattaat gtcatcctcg tcacttccgg gcgaaatgag
     1021 ggccctgcaa tcgcccgcca ccttgtggaa aagaggatgg ctgcatgcgt gaacatcgcg
     1081 gaaatcaggt cctgctatcg ctgggaaggg aggttctgcg atgatcccga agtgcttctc
     1141 atcataaaga ctgcacacga gaaggtcgag gaagcccttg ccgccatcag ggaggtgcat
     1201 ccatacaccc tccccgagat gatcgtgctc ccggtcgttg cagggtatcc cccctatctt
     1261 gcctgggttg aagaggagac cactcgatga acactgtttc catcagtggc atccaggtca
     1321 ggaacggcag gcccggagag atagaggaca ccgtggtgcg ggagagccgg ttctcccttt
     1381 acctgaacgg gacgtatttt accagtatgg ttgcaagcaa cgaccagctc gatgaactcg
     1441 gtgcaggttt cgtgacgtgc cagggaatct ccggcagggt tgatgaggtg agggtgaagg
     1501 gagaagagat ccatgtcatt gcccccatca cgggagaact gctccaggag atcatctcca
     1561 caggggcgct gggggtcagg aggccttttc tccccgtcac atcgtctctt accctcacca
     1621 ccgatgacgt gtaccgtatc acgggggaga tcgagaccga gacgtggcgg cggaccggcg
     1681 gcgtccactg ctcggtcctc ttccacgaaa acgagcttct cgtgaaggca agcgaccttg
     1741 gaaggcacag cacggtcgac aaggtggtgg ggcatgcagt gatcaggggt attgacaggt
     1801 cgaggtgcgt ccttggctgt accggaaggc agccgaggga catggtgaca aaggccgcgc
     1861 atgccgggat acccgttgtc gtctcccgtg cggcgtcgac agagcagggg atagaaacgg
     1921 caaggaaggc aggaataacc ctcatctgct tctcccgcgg agacaggttc acagtctaca
     1981 cgcacccgga aaaggtcagg gatcttcggg ggtacctcaa tcaggcaggc gtccggaatg
     2041 ggaagtaaca tagccatggg gatcgttaat tagtcaggca gggaggagat tcatcatcgc
     2101 tgcaggaaca gaaaagaagg acgcgctcct ggtcctgggg tgcccgcagg tcccggtcca
     2161 gtccccggtt gccctgtacc tcatgcaaag gcttcggatg ctcgggatat caccggtcgt
     2221 cgcaggcaac caggccgccc gcatgcaggt cgaggtatca gacccgctcc accattattc
     2281 aggggaaatg gtggacctcg atacctgcat cgcggacctg gcggagggaa ggagaagcta
     2341 ttcgtactac atgatcttcg tgcacaacga tgccggggtc tcgtatgctg caaccgtcca
     2401 ggcaatcacc gggaagaagg tggtagccat cctctatggg gagcacttca gggaggttgg
     2461 agagactatc ggcttcccct gcgagaaggt cgctgcaaaa gcagtgcaca atcccatgcc
     2521 actcaaaaag aagatagacg aggtgcttcc gtgggttgta tcgaatcttt gaagtacgag
     2581 attgtcctca ggggggcatc gtttgcagaa tgcaggggct atgttgagga gcactgcagt
     2641 gagcgggtga tcgtggatcc cgggttcaag atcttcgaga aacctatcat aggggtgcct
     2701 ccggtcgtca tcgggcttga cggggacgtg atcactttcc cgtatacgaa gccctgctat
     2761 ggcacgttcc ttctcaagac tgaaaaccct cccgaagcgg cccggatacg gtcccttggg
     2821 aagaaataac atcaccatcc agtttttttt tataggaaat cgcacctgcg atttcgaatg
     2881 aaaatcactt cgtgattttc atcatttata ttctacggtg gggccgtcat aagcgattcc
     2941 tatgaaaata gctgcggcat tttcatgcgt cgggcttgtt gcaaattgca tcattcttgt
     3001 tttcatctta tgagcatgtg tcttgaaagg gaatcgtgtg gttttttggg aagaacctat
     3061 atctttccct gaaaagag
//
LOCUS       DAOQVJ010000152         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_37343_length_3055_cov_4.181000, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000152 DAOQVJ010000000
VERSION     DAOQVJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37343_length_3055_cov_4.181000"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKL26_04210"
     CDS             complement(<1..262)
                     /locus_tag="PKL26_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS82933.1"
                     /translation="MTKSNVLQERIIFPKGEKIAGEGIANDYFTGTVWLQMLVPPEKI
                     FNCPVYNVTFEPGARNNWHKHPGGQILLVTGGRGYYQEEGKPA"
     gene            complement(395..937)
                     /locus_tag="PKL26_04215"
     CDS             complement(395..937)
                     /locus_tag="PKL26_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNS82934.1"
                     /translation="MNTNDSKCLVAYFSRPGNNYVGGTIVNLQVGNTEVVAKMIQELT
                     GGDLFRIEAVHAYPEDYTETIEIAKQEMHTNARPKLTSRLECVDSYDEIFLGYPIWWG
                     TMPMPVFTFLEEHNLSGKTIAPFCTHEGSGLGRSVTDIKKICQQSTVREALAIRGGDV
                     KNAKEAVAGWLREIGVNRDQ"
     gene            1062..1415
                     /locus_tag="PKL26_04220"
     CDS             1062..1415
                     /locus_tag="PKL26_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82935.1"
                     /translation="MEETPKKYKYSWPIEATLDVIGGKWKPLVMYQLKDGTLRFSEIM
                     DRVHPKITQRMLTKQLRELENDGLIIRKVYPQVPPKVEYSLTEKGRSVMPILDDLCDW
                     GSEHMADEIEFKCED"
     gene            complement(1747..2853)
                     /locus_tag="PKL26_04225"
     CDS             complement(1747..2853)
                     /locus_tag="PKL26_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNS82936.1"
                     /translation="MEPYVPQTLPIETIDWIRHVPLIGKANRALARYDGLVQGIVHPE
                     ILLSPLTMREAVLSSRIEGTQASLEEVLEFEAQAKAKYETEKEKDIQEIINYRLAMNS
                     ALELLNERPITINMIRELHRILLTSVRGRDREPGQIRRLQNYIAAPGTPIERAIFVPP
                     APPQLMDALTNWEIYLHADEKDPLVQLAVLKAQFELIHPFRDGNGRIGRMLVPLILYS
                     KGVLSRPIFYISAYLDHHRDEYYARLLGVSRDEDWDGWIAFFLNALVEQAADNTHKAR
                     AILDLYEELKISVPEATRSPYAIQAIDAMFSRPVFKRTDFIAESHIPKQSAYRILQNL
                     QKNGILTEIQEGRGKRAAIYSFPRLLDITEGTIP"
BASE COUNT          702 a          759 c          791 g          803 t
ORIGIN      
        1 tggcaggttt gccttcctcc tgataatatc ccctgccacc ggtcacgagc aggatctgtc
       61 cgccgggatg cttgtgccag ttgttccgtg cacccggttc aaatgtgaca ttgtataccg
      121 ggcagttgaa gatcttttcc ggcgggacca gcatctggag ccaaaccgtc ccggtgaaat
      181 aatcgtttgc aattccttca ccggcaatct tttccccttt tgggaaaata atccgttctt
      241 gtaaaacgtt tgatttcgtc ataagacatc atctcctgat ttttggtaag agtgacattt
      301 ggttatctat acagccgaag ctactcgcag agacttccaa tccgttcctt cctaatgttc
      361 gtgtgtgcat aggttgacct cctttagaac atgatcactg atcccggttc accccgatct
      421 cccgcagcca cccggccacg gcttccttcg cgtttttcac gtcaccgccc cggattgcaa
      481 gggcctcacg aacggttgac tgctggcata tcttcttgat atcagtcaca ctccgcccaa
      541 ggccgctccc ttcgtgcgtg cagaacgggg cgatggtctt cccggagaga ttgtgttcct
      601 caaggaaggt gaagaccggc atcggcatcg ttccccacca gataggatag ccgagaaaga
      661 tctcatcata agaatccacg cattccaacc ggctggtaag cttcggtctg gcattcgtgt
      721 gcatttcctg cttagctatt tcaatggttt cagtatagtc ctcgggatac gcatgcactg
      781 cttcaatacg aaacagatcg cctccagtca attcctggat catcttggct acaacttccg
      841 tgttgccgac ctgcaggttt acgatagttc cgccgacata gttgtttccc gggcgcgaga
      901 agtatgcgac caggcatttt gaatcatttg tgttcatttt catctcttcc acctatgacc
      961 cccgggcggt atcgctcgtg ttcctgtagt atatattaag tatgtatgac atatatagta
     1021 taaacaaaca tatatgtgat atacttacag gattgatact tttggaagaa actccgaaaa
     1081 aatacaaata cagctggccg atcgaggcta cactggacgt aatcggcggc aaatggaaac
     1141 cgctcgtaat gtaccagctc aaggacggga cacttcggtt cagcgagatt atggaccggg
     1201 tgcaccccaa gatcacgcag cggatgctta caaaacagct ccgcgaactg gagaacgacg
     1261 gactcatcat acggaaggtg tatccccagg ttccgcccaa ggtggagtat tcgctcacgg
     1321 aaaagggccg gtcggtgatg ccgattctcg atgatctctg tgattgggga tccgagcaca
     1381 tggcggacga gatcgaattt aagtgcgagg attaactaaa agaaatcgac gaattactca
     1441 ttacactgga tgaggcattg aaccttcttt gcacaaactc cgggctgagg catgattggt
     1501 attttaccaa caattttcgg ttataatcaa atttaaagcc tatatgttgc gtatttttaa
     1561 tattttttcc gattggcaga tcaatctggt tactaagtgc actctgaaat agaagacttc
     1621 ccttcgaccc agatgagatt gggcttttga catgttctct cgttgtgtaa cacattgcat
     1681 gatatggtgt tacacaagcg gtttaatgtg ctacaagtgt tacacaaggg gatcctggca
     1741 cgtggattat ggaatcgttc cttccgtgat atcgagtaaa cgcgggaagc tatagatggc
     1801 tgcacgtttc ccgcgtccct cctgaatctc agtgaggatt ccattcttct gcaggttttg
     1861 aaggatccgg taagcgctct gtttcgggat gtgggattct gcaatgaaat cagtcctctt
     1921 gaagacaggg cgggaaaaca tcgcgtcgat tgcctggatt gcatagggag agcgggtagc
     1981 ctctgggaca ctgatcttca gttcctcgta gaggtcgagg atggccctgg ctttatgggt
     2041 attgtcggca gcctgttcga cgagcgcatt gaggaaaaat gcaatccatc cgtcccagtc
     2101 ctcgtcgcga gatactccaa ggaggcgtgc ataatactca tcccggtggt gatcgagata
     2161 cgcactgata tagaatatcg gccgggacag gacccccttg ctatacagta tcagcggaac
     2221 cagcatccgc ccgatcctcc cgttaccatc gcggaacgga tggataagct cgaactgggc
     2281 cttgaggacg gcgagctgga caagcgggtc tttctcatct gcatggagat agatctccca
     2341 gttggtgagt gcatccatca gttgcggagg agcgggtggg acaaagatcg ccctttcgat
     2401 gggtgtccct ggagcggcga tataattctg caggcgcctg atctgcccgg gctcccggtc
     2461 ccgcccgcgg acactcgtca ggaggatccg gtgaagctcg cggatcatgt tgatggtgat
     2521 aggtcgctca ttgagaagtt caagggcgga attcattgcg agccggtaat tgataatctc
     2581 ctggatatcc ttctccttct cggtctcgta tttcgccttg gcctgggcct cgaactccag
     2641 gacttcctcg agggaagcct gggtgccctc gatacgtgaa gacagtaccg cttcccgcat
     2701 cgtcaatggg gagaggagga tttcgggatg cacgatgccc tggacgagtc cgtcataccg
     2761 tgccagcgca cggttcgctt tccctatgag cgggacgtgc cgtatccagt ctatggtctc
     2821 gatcggcagg gtctgtggga cgtagggctc cattgttgat gaccgggctc tcttcctata
     2881 ttcatgatct tgttctggag gtaaattagt tggtggaaaa atttgatcga actcactccg
     2941 gataatcgtt gttctttaaa gaaatataca gggcggcagg tccgggaatc accagatgcg
     3001 gatacttata ctttgcaact tcagttcgcg cgagtgtaaa tcggagcgat tttca
//
LOCUS       DAOQVJ010000153         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_37363_length_3054_cov_3.397132, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000153 DAOQVJ010000000
VERSION     DAOQVJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37363_length_3054_cov_3.397132"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..909
                     /locus_tag="PKL26_04230"
     CDS             301..909
                     /locus_tag="PKL26_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS82937.1"
                     /translation="MNYRVFLLVALCISGIGVSCATSAPPLEAIDAYNHGIDLAVEGN
                     FSAALGSIDAALSMDPNFTVAWITRAGILNAMGDYNQSLEASDRALAIDENQAEAWTN
                     RASALISLGRNEEALEAADRAIELDPRLSEAWIDRSTALSNLGRTEEADEAVQMAMFL
                     IGELENSPVPQTTTAKAPLHWLLVPLGTGAALGFYSKKRYIP"
     gene            complement(1252..1710)
                     /locus_tag="PKL26_04235"
     CDS             complement(1252..1710)
                     /locus_tag="PKL26_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HNS82938.1"
                     /translation="MKVMVGGTFDPLHDGHKRLISRSFELAGNGGEVTIGLTTDIFAS
                     RKSHPVRPFNERMESLERFIRENRWNAAWSIEPLNDRFGSALVAEFDALVVSEETLPI
                     ARDINRIRREKGRSKVDIHQITCVLADDGKWISSTRIYRGEIDDHGRLLT"
     gene            1741..2232
                     /locus_tag="PKL26_04240"
     CDS             1741..2232
                     /locus_tag="PKL26_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNS82939.1"
                     /translation="MAVGRTRTAETFVAENATVLGDVKLGLGVSIWFGAVVRGDRDKI
                     VISDRSNVQDNAVVHTSKGFPARIGEDVSIGHGAILHGCTISDRVLVGMGAIVLNGAV
                     VGEDCLIGAGSVVTEGTTIAPGSVVVGVPGKVIKQVTEAQRQHILQNARNYWEMAGSY
                     LHV"
     gene            2225..>3054
                     /locus_tag="PKL26_04245"
     CDS             2225..>3054
                     /locus_tag="PKL26_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS82940.1"
                     /translation="MSDVVIVGGGVTGIQAALDLANHGIEVHLVEKEPSIGGHMAMLD
                     KTFPTNDCSMCILSPKMMDVLRHPRIHIHTCAKVSAISGKAGQFSVTITKMPRYIDEE
                     RCNGCGDCIDICPVEVYNRFDAGIGVRKAIYKPHQQIVPDIVVKDPEHCIECGLCYDV
                     CGREAIRKEDTERKITVNAASIIIACGYDVFDAGNKEQFQYLRYPDVITSLEFERMMN
                     ASGPTGGKVRRLSNGKTPGSIVFVQCVGSRDMQIDRPYCSGVCCMYAIKNAMLIREKY
                     P"
BASE COUNT          726 a          809 c          854 g          665 t
ORIGIN      
        1 actctcatat accggcgatc tgtgcaatct gagcggtaac caggggtcaa accacctgcg
       61 agcctttcct gcaggatgcc catcatcctg ttgttttatt ggggaaatgc atggtgcggg
      121 ttttttcccg gcggtccgat gcatactgaa aacggctgtt cgggtcgcca ttctcaaacc
      181 ctggaacatg gcccaggcca tagcctgaca gcatatgcac ctgcggatcg cggctgaagg
      241 gaaaacagaa agtatttccc gttcatacat aaaaccgaat agaaaggaca ggatttttcc
      301 atgaattacc gagttttcct tctggttgcg ttgtgtatat caggtatagg ggtctcatgt
      361 gcaacatcgg caccgcccct ggaggcaatc gatgcataca accacggtat cgatcttgct
      421 gtggagggga acttctccgc agcgctcggg tccatcgatg cggcactctc catggatccg
      481 aacttcaccg tcgcctggat cacaagggct ggcatcctga acgcgatggg agactacaac
      541 cagtcgctcg aggcttcgga cagggcgctt gccattgacg agaaccaggc agaggcctgg
      601 accaaccgcg caagtgccct catctccctc gggcggaacg aagaggcact cgaggccgcc
      661 gatcgcgcca tcgagctcga cccacgactc tcggaggcct ggatcgaccg atcaaccgca
      721 ctctccaacc ttggccggac agaggaagca gatgaagcgg tgcagatggc gatgttcctc
      781 ataggggaac tcgagaattc ccccgtcccc cagacaacca ctgcaaaggc acctctccat
      841 tggctcctgg tccccctggg aaccggagcg gcactcggtt tttactcaaa gaagagatat
      901 atcccctaat ctgtttttac cggtgcgtct tggaaaggaa tgtgaaaatt gagaaaaaat
      961 gatgctgaga gtatcgatct caaagttagg ggtttcctgt tatcaatgta catgatatga
     1021 tcggttcaac tgtcattcct ggacattcgc aaatgctata aagagagaat ttttccagcg
     1081 taggacgaga gacagaccct cctgtcgcga ggagagcacg aagaaaacca cactcatgga
     1141 aaagagatgc tgtgagtatc actcacaccc tcgtccccat ctccatataa attctgttga
     1201 tggctgtggt tcaacgaatg aaaaagtaca tgtgagggct gggagggatg ttcaggtgag
     1261 cagcctgccg tgatcgtcga tctccccccg gtatatcctc gtgctggaga tccattttcc
     1321 atcgtcggca aggacgcagg tgatctggtg gatgtcgacc ttcgagcggc ccttttccct
     1381 acgaatcctg ttgatgtcac gggcaatggg gagagtttct tcactgacca ccagggcgtc
     1441 gaactccgcg acgagtgccg acccaaaacg gtcgttcagt ggctcgatcg accacgctgc
     1501 attccagcgg ttctcccgta tgaaccgctc cagtgactcc atcctctcgt tgaacgggcg
     1561 aacggggtgg ctctttcgtg atgcgaagat atcagttgtc aggcctatgg tcacctcgcc
     1621 gccatttccc gcaagttcaa aagaacggga gatcaacctt ttatgaccgt catgcagagg
     1681 atcgaatgtt cccccgacca taaccttcat ggtgccacat attgattttt ttatgctatt
     1741 atggctgttg gtcgcacgcg aacggcagag acctttgttg ctgaaaatgc cacggtgctc
     1801 ggggatgtga aacttgggtt gggagtgagc atatggttcg gcgcagtggt ccgtggagac
     1861 agggataaga ttgtcatatc agaccggtcg aacgtacagg acaatgctgt agtccatacc
     1921 agcaaggggt tccccgcgcg cataggagaa gatgtgtcca tcggccacgg ggccatcctg
     1981 cacgggtgca ctatatcgga cagggtcctc gtgggaatgg gggccatcgt gctcaacggg
     2041 gcagtggtgg gtgaggactg ccttattggt gcaggctcag tggtcacgga agggaccacg
     2101 atcgcccctg gatccgtggt ggtaggtgtc ccgggaaaag tcatcaagca ggttacggaa
     2161 gcccagagac agcatatcct ccagaatgcc aggaattact gggagatggc agggagttac
     2221 ctgcatgtct gacgtggtga tcgttggcgg gggggtcacc ggcatccagg cagctctcga
     2281 cctcgcaaac cacggaattg aagtccacct cgtggagaaa gagccctcca tcggcggtca
     2341 catggcgatg cttgacaaga cattccccac gaacgactgc tcgatgtgca tcctttcccc
     2401 gaaaatgatg gacgtcttgc ggcaccctcg catccatatc cacacctgcg cgaaggtgag
     2461 tgcgatctct ggtaaggccg gccaattttc tgtcaccatc accaaaatgc cccgctatat
     2521 cgacgaagaa cgctgcaacg ggtgcgggga ttgcattgat atctgcccgg ttgaggtata
     2581 caaccgcttc gatgcaggta tcggggtccg gaaggcaatc tacaaacccc accagcagat
     2641 agtccccgat atcgtggtca aagatcccga gcactgcata gagtgcggcc tgtgttacga
     2701 cgtctgtggc cgcgaagcca tacgaaaaga ggatacagag cgcaaaatca ccgtgaatgc
     2761 ggcaagcatc atcattgcct gcggatatga tgtatttgat gcgggaaaca aggagcaatt
     2821 ccagtatctc cgttatcccg acgtgatcac gagcctcgag ttcgagcgaa tgatgaatgc
     2881 cagcggaccg accggtggga aagtccgaag gctctcaaat ggaaagacac cgggctcgat
     2941 cgtattcgtc caatgcgtgg gttcaaggga catgcagatc gacaggccat actgctctgg
     3001 tgtctgctgc atgtatgcca tcaagaatgc catgctgatt cgggagaagt accc
//
LOCUS       DAOQVJ010000154         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_37463_length_3049_cov_4.626253, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000154 DAOQVJ010000000
VERSION     DAOQVJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37463_length_3049_cov_4.626253"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKL26_04250"
     CDS             <1..944
                     /locus_tag="PKL26_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82941.1"
                     /translation="ANPRHVAIIGEPMAGKTTVAGEIVRRYGDCIHYVTLGGVMTQDA
                     MPAFSSIQKDCILVDNCEFFATRQIGGFKFLDDFLKCQFSSTKLFITTWNSYAWRYLS
                     AVRNIGTYYPLVVTLPAMDSSTLKQVILSNYPQREIRFVENPPSEPASICTMVQRQAR
                     LPFSGREVSIPWFHLNTNAIRSRMQVGRETVVSPEDAVFEKIRRIAQGNPGVAMQIFS
                     GCLEDDAVSSEKIPENECELSLDITESFVLTLILSMKSLHYRDFSAMAGGGDVLDRAL
                     FSMRRQGLIFEDDGVYRISPARLNCIGNYLKKTRRVW"
     gene            946..1776
                     /locus_tag="PKL26_04255"
     CDS             946..1776
                     /locus_tag="PKL26_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNS82942.1"
                     /translation="MADSIFSDVTGELSLKTIDAGLILHLVAIVVVAYLLTTLLSYIL
                     SRFSERAGVYRQLVTLFIPLLKIFIYTVALYLFITALIEPSVTQLIAFAGLFGAVVGI
                     GLKDLFSDLAGGIVIILEKPFQIGDKVTMGNYYGEVKDIKLRSTRIQTPADELVSVPN
                     NTIFTQSVNSGNAGDIAMMIVIDLFIDSMSDAAHAAKILKEALVTSKYVIISEQYPYT
                     ILIEDFPCYTRVRAKGYVYDLRLEFEFKSEVTRRALAEFKRSDIKLPLCYPGLDGLIS
                     "
     gene            2176..2652
                     /locus_tag="PKL26_04260"
     CDS             2176..2652
                     /locus_tag="PKL26_04260"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HNS82943.1"
                     /translation="MHGPEHHFLVPALLLTSYSLVTGEREALGGRLKRARERAAMVRG
                     GSCGTHGACRAAIGTGIFVSIITGSTPLSWREWMLSNLMTGRSLHEVALAGGPRCCKR
                     NTYIAILAAVSFVKEEWGIPIPVRRKLACEFSDLNRECRLAECPFYPGGDGGHQDA"
BASE COUNT          705 a          796 c          789 g          759 t
ORIGIN      
        1 ctgccaaccc gaggcacgtg gccatcatcg gtgaaccgat ggcaggcaaa acaacggtcg
       61 ccggtgaaat cgtgcgcagg tatggcgatt gcatacacta tgtaaccctt gggggcgtca
      121 tgacccaaga tgcgatgccc gcgttttcat caatccagaa ggattgtatc cttgttgata
      181 actgcgagtt tttcgcgacc aggcagattg ggggatttaa attcctcgat gatttcctga
      241 aatgccagtt ttcctctaca aaactcttca tcaccacgtg gaacagctat gcatggcgat
      301 acctctccgc agtcaggaac atcgggacct actatcctct cgtggtcacg cttcccgcta
      361 tggactcctc aactctcaaa caggtgatcc tctcaaatta tccccaaagg gagattcggt
      421 tcgttgaaaa ccctccctcc gaaccggcga gcatctgcac catggtccag cgccaggctc
      481 gtctgccatt ctccgggagg gaggtatcca tcccctggtt ccatctgaat accaatgcaa
      541 tacgatcgcg gatgcaggtc gggagagaga cggtggtgtc accggaagat gcagtatttg
      601 aaaagatacg ccggattgcg caggggaatc ccggggtcgc gatgcagatc ttctccggat
      661 gcctggagga tgacgcggtc tcctctgaga agataccaga aaatgaatgt gaactctctc
      721 tggacatcac agaatctttt gtcctcaccc tcattctgtc catgaagtcc ctgcactacc
      781 gcgatttctc tgccatggca gggggcgggg atgtgcttga ccgggccctg ttcagcatga
      841 ggcggcaggg gctcatattt gaagacgacg gtgtgtaccg catatctccg gcgaggctca
      901 actgcatcgg gaattacctg aagaagaccc ggcgggtgtg gtaacgtggc agactcgatc
      961 ttttctgatg tgacgggtga gctctcgctg aaaaccattg atgccggcct cattcttcac
     1021 cttgttgcca tcgttgttgt cgcgtatctc ctcacaaccc tgctcagtta cattctcagc
     1081 aggttttcag agcgggcagg tgtatatcgg cagctggtga ccctgttcat cccgctcctg
     1141 aagattttca tctatacagt tgccctctac ctcttcatca cggctctcat cgagccttca
     1201 gtcacgcagc tgattgcatt tgcaggtctc ttcggggccg tggtcggcat cgggctcaag
     1261 gatctcttct cagatctggc agggggcatt gtgatcatcc ttgaaaaacc cttccagatc
     1321 ggcgacaagg tgaccatggg gaactattac ggagaagtga aggatataaa gctccgctcc
     1381 acgagaatcc agacacctgc cgatgaactc gtctcggtcc cgaataacac catttttacc
     1441 caatcagtca acagtggcaa tgccggcgac atcgcgatga tgatcgtcat cgatctcttc
     1501 attgattcga tgtctgacgc agcacacgcc gcaaaaattc tgaaagaggc tctcgtgaca
     1561 tcgaagtatg tcatcatatc agaacaatat ccctacacga tactgataga agattttcca
     1621 tgctataccc gcgtccgtgc aaaaggatat gtatacgatc tcagactgga atttgaattc
     1681 aagtcggaag tgacgcggag agcacttgca gaattcaagc gctcggatat caaattgcct
     1741 ttatgctatc cgggcctgga cggtctcatc agttgaggcg gattgcgcct tcattttccc
     1801 aattttttcc gtcttccggc gtttgtgcca gacattggat gcattgtttg tcaatcgtgg
     1861 tccagcaatt acacttatcg atacattcat gcctttttag gggtgttttt tgggagagaa
     1921 gaggattcac cttggaaatg aaaagcgggc gcctggtatg cgggagggac cttgtctatc
     1981 tggatgcacc cgaagagctt tttttctgcg gcaaatgacc tgtcactgaa gtgagatgcg
     2041 ttgagggtca tttcatctgc gatgactgcc accggcatcc tgcagaatac ctcatagaga
     2101 cgacgtgcct tggtacaaat attgtcgatc ccatcctcct tgcaatctcc ctgatgcgac
     2161 atccggcatt caaaaatgca cggacccgaa caccatttcc tcgtcccggc cttactcctt
     2221 acaagttatt cccttgtcac cggagagcgt gaagcgcttg ggggaaggct gaagagggcc
     2281 cgggaacggg ctgccatggt gagaggagga tcgtgcggga ctcatggggc ctgcagagcg
     2341 gcaatcggga cgggtatctt cgtgagcata attacgggat caacacccct ctcctggaga
     2401 gagtggatgc tctccaacct gatgactggc cgaagtctcc atgaggttgc acttgccgga
     2461 ggaccacggt gctgtaaaag aaacacttac atcgcaatcc tcgctgcggt gtcctttgtc
     2521 aaagaggaat ggggaatacc catccctgtc aggaggaaac ttgcctgcga attcagcgat
     2581 ctgaaccggg aatgcaggct ggcggaatgc ccgttttatc ccggaggtga cggagggcat
     2641 caggacgcat agagaacaca ccggggcttc ctgcggtcct ggaaatatat attccacatc
     2701 ctgttggcag ggatgcgtgt tcaggacaca tatgacaagc ccgggaatcg gccaggacat
     2761 cccctgtttt tcccccaaaa ttccttcaaa ggaggatgtt ctctctgcaa tctatgatta
     2821 gacggacttt ggcggtcgat tcacttttaa gagctgaacg attccattag cattcatacc
     2881 tttcctcatg gaaaggaaat gagagcgatc ttgtatggtg aggaagtatt cacgaccctg
     2941 tattgtcgtc agccgttgca tcgagtttga cccctgccgc tatgacggtt ctcggatacc
     3001 ttctcctgca gtggaccagt tgaaatcctt tgttgacttc ataccggta
//
LOCUS       DAOQVJ010000155         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_37521_length_3046_cov_3.482113, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000155 DAOQVJ010000000
VERSION     DAOQVJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37521_length_3046_cov_3.482113"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PKL26_04265"
     CDS             <1..1308
                     /locus_tag="PKL26_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS82944.1"
                     /translation="LPVIFRPTTRICHSKGDTLVESPVSAPRKGEFHKNPQQYVVIPA
                     HTRVLHRKLNQKQPQIKRALVEKGYNHAEVRGSVAVVASGIAAAYVREVLPPDVSFMK
                     IGAYPIDEEWLGGFVRKHESVLVIEELHPVVEEAVRQVSGGVAVHGKKDGMVPYEGEL
                     SPVIVARVMQACGIAPTREFPNPVPAGDIPNRPPILCAGCMHRPVFYAMRKVFKDGIF
                     PSDIGCYTLGLQLGAVDTTICMGASVTVASGIAHSGEEREVVATIGDSTFLHTGIPGL
                     LNAVYNGAKMILVILDNRITAMTGHQPNPSSGTNAFGVPVPPVSLEAICRACGVSYVE
                     TVDPYDLVSLLSVMKDARERPGVKVIIARQPCVITARRAGVRRGRFQVDAESCTSCGL
                     CIKFGCPAIEKSGEKAHVNDLCSGCGVCVQICPAGAIRKEVKR"
     gene            1305..1910
                     /locus_tag="PKL26_04270"
     CDS             1305..1910
                     /locus_tag="PKL26_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNS82945.1"
                     /translation="MSGSYDVLMVGIGGQGIILASNILGEACLIEGRNVRGAETHGMA
                     QRGGSVEGHIRIDGKFGPLIAPGTAQLMIAFDMLEALRYSHFLAPEGHMVVNSHIVIP
                     TSVFMQGAAVPEKEAVQERLKSRALCLVDADALAVEAGSPLSQNVVMIGAASWFMPFA
                     PASLLQAVERLVPKKTVEINRKAFELGRAAGERCREGSSDT"
     gene            1907..2692
                     /locus_tag="PKL26_04275"
     CDS             1907..2692
                     /locus_tag="PKL26_04275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNS82946.1"
                     /translation="MSPHDLGPFSPDTIYHGDALALLPLLRGGSIDLIVTDPPFAIDF
                     RAKKMNYNRTGSRVIEGYREIPPGEYGAFTSRWISEAFRVLSPTGSLYVFSGWNRLKE
                     VLCSLDEAGFSTINHLIWKYQFGVFTRRKYVTSHYHILFAVKDPGHYTFHKVEHYPED
                     VWVINREYWKGKKKTPTKLPPQLVRKILSFSSSPGDLVLDPFLGSGTVAAVSQEMGRR
                     FLGFEVVDEYYRFALERLEECGSAGARERDGSGGRTEHAEGEN"
     gene            complement(2674..2757)
                     /locus_tag="PKL26_04280"
     tRNA            complement(2674..2757)
                     /locus_tag="PKL26_04280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2723..2725),aa:Ser,seq:gga)
BASE COUNT          607 a          916 c          942 g          581 t
ORIGIN      
        1 ctgcccgtca ttttcaggcc caccacgcgc atctgccatt ccaaggggga taccctggtg
       61 gaaagccccg tcagcgcccc caggaagggg gagttccata aaaaccctca acagtacgtg
      121 gtgatccccg cgcatacccg ggtactccac cgcaagctga accagaagca gccgcagatc
      181 aagagagccc ttgtggagaa ggggtacaac catgcagagg tgcgaggctc cgtggccgtg
      241 gtcgcgagcg ggattgccgc ggcctatgtc cgggaggtgc tcccccccga tgtatcgttc
      301 atgaagatag gcgcgtatcc catcgacgag gagtggcttg gcggtttcgt gagaaaacac
      361 gagtcggtcc tggtaatcga ggagcttcac cccgtcgttg aggaggccgt gcggcaggtc
      421 tccggaggtg tcgcggtgca cgggaagaag gacggcatgg tgccgtacga gggggaactc
      481 tcgcctgtca tagtggcaag ggtcatgcag gcctgcggca tcgccccgac aagggagttt
      541 cccaatcctg tccccgccgg tgatatcccc aaccgccccc ccatcctctg cgcgggatgc
      601 atgcaccgcc cggtctttta cgcaatgagg aaagtattca aggacgggat atttccaagc
      661 gacatcgggt gttacaccct gggcctccag cttggtgcgg tggataccac catctgtatg
      721 ggtgcctccg tcaccgtcgc gagcggcatc gcccattcgg gggaagaacg ggaagtcgtc
      781 gcaaccatcg gggactcgac attcctgcat acaggcatcc cgggactctt gaacgcggtg
      841 tataacgggg cgaagatgat cctcgtgatt ctcgataacc ggatcacggc tatgaccggc
      901 catcagccga atccttcaag cgggacgaat gcgttcggtg tccctgtccc cccggtctct
      961 cttgaggcta tctgcagggc atgcggagtc tcttatgtgg agactgtgga cccttacgac
     1021 ctcgtctcat tgctctcggt aatgaaggat gcccgtgagc gccctggcgt gaaggtgatc
     1081 atcgcccgcc agccgtgcgt gataaccgcc cgccgggccg gcgtgcgccg cggccggttc
     1141 caggtcgacg ccgagagctg cacatcatgc gggctctgca tcaaattcgg gtgccccgcc
     1201 atcgagaaat ccggcgagaa agcgcatgtc aacgacctgt gcagcgggtg cggggtatgc
     1261 gtccagatct gccctgcagg cgcaatacga aaagaggtga agagatgagc gggagctacg
     1321 acgtcctgat ggtgggaata gggggccagg ggatcatcct cgcctcgaat atcctcggcg
     1381 aggcatgcct cattgagggc aggaatgtcc gtggtgccga gacgcacggc atggcccagc
     1441 gaggaggctc ggtggagggc catatacgca tcgacgggaa gttcggaccc ctcatcgcgc
     1501 ccggcaccgc acagttaatg atcgcgttcg acatgctgga ggcgctccgg tactcccatt
     1561 ttcttgcgcc cgaaggacac atggtcgtaa acagccacat tgtcatcccc acttccgtct
     1621 tcatgcaggg agcagcggtt ccggagaagg aggcggtgca ggagcggctc aaaagccgtg
     1681 cactctgcct ggttgacgca gacgcccttg cggtggaggc gggaagcccc ctctcgcaga
     1741 acgtggtaat gatcggcgcc gcttcatggt tcatgccctt tgcccccgcc tcccttctcc
     1801 aggcggtcga gcgcctggtc ccaaagaaga cggtggagat caacaggaag gcattcgaac
     1861 tcggaagggc tgccggcgag aggtgcaggg aagggagcag cgacacgtga gcccgcacga
     1921 tctcgggccg ttctccccgg acaccatcta ccacggcgac gccctcgccc tcctccccct
     1981 tctccgcggg gggagcatcg acctcatcgt cacggacccc cccttcgcaa ttgacttccg
     2041 ggccaaaaag atgaactata accggaccgg ttcgcgcgtc atcgaggggt accgggagat
     2101 cccgccagga gagtacgggg catttacctc ccggtggatc agcgaggcgt tccgggtact
     2161 ctcccctacg gggagcctct acgttttttc agggtggaac cggttaaaag aagtgctgtg
     2221 cagcctggac gaggcaggtt tttccaccat caaccacctg atctggaagt accagttcgg
     2281 cgtgttcacc aggagaaagt acgtcaccag ccactatcat atcctcttcg ccgtgaaaga
     2341 ccccggccac tacactttcc acaaggtcga acactatccc gaagatgtct gggtgatcaa
     2401 tcgcgaatat tggaaaggga agaagaagac ccctaccaaa ctcccccccc agctggtgcg
     2461 gaagatcctc tcgttttcga gcagcccggg tgatctggtc ctcgatccat tcctcggttc
     2521 cggcaccgtg gccgcagtca gccaggagat ggggcggcgt ttcctcgggt tcgaggtggt
     2581 cgacgaatat taccggttcg cgctggagcg gctggaagaa tgcgggtccg cgggtgcgag
     2641 ggaaagagac ggcagtggcg ggagaacaga gcacgctgag ggggagaatt gaactcccga
     2701 ggggcaaaag ccccacaggc tctccaggcc tgcgccgtac cgctagacta cctcagcaaa
     2761 aggtgtatcc atactatggg acgggtcttt ttttaatggt atctcatctc cagggcaggg
     2821 acgatgagac cgcgcggaaa ggccggatgt ccggggaagg tttttcaggg cttttttccc
     2881 cgtttctcat cgcttctcct tctttttcca gcccttcggg tatgtccctg gccgcatgaa
     2941 gacggtccgc ggcgccgcca cgagccctgt ctgtcccggt gcaacctgag ttgaggagac
     3001 gatggcctcg ccgatgcaga ccagttctcc tttttctgtc agcacc
//
LOCUS       DAOQVJ010000156         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_37601_length_3041_cov_4.303416, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000156 DAOQVJ010000000
VERSION     DAOQVJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37601_length_3041_cov_4.303416"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKL26_04285"
     CDS             <1..990
                     /locus_tag="PKL26_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82947.1"
                     /translation="HEEYDTAKIIGEFGLILKDIGGPSVIGSMALSEIFAAFEEAPMI
                     GAGFSGGPVNPPLGHLEGDCVPALRLLMKHHGDIFAVAEVIRDYKMNSFIDPEMALCG
                     LNTISRKAEQVTRGKITRACILASEGVRDRAIYRRAAHTYEIITGGKSVADATRILDE
                     ERKNYVERRGSAILSAFTGKKIELKFTAIKPHGRRTDKFTERYWGFDSNVSYDVTIDG
                     KKYHVENLSGKEVPDFALKGKNRDNPDWPVALFCGAVLTQELQYIGHTIINITVPAAV
                     GAILGMDAGEAADKAEDGAFLTRAIPGAGDKAKDVAKLAQRVYAKINEPFPPQ"
     gene            991..2196
                     /gene="larC"
                     /locus_tag="PKL26_04290"
     CDS             991..2196
                     /gene="larC"
                     /locus_tag="PKL26_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNS82948.1"
                     /translation="MKALLIDPRTGGIAGDMLCAALCDLTGSEKPLQEVADAITGLSR
                     CSTFEISVERTVLPCSAACISVAIVEDDGPGTDVSRELNEVISTIGLSPSGTAMAHAI
                     LNDLISVKDRFHPETATGQTFASLDTIFDILGPLALLEEAGLLDCPIYTTPPALGGGL
                     IHTPGGDVGGPAPASLEICARHRMPVSDSALSMELTTPTGAALLANLARVVTLFPAMT
                     PLRTGYGAGAGDHGSSCRILRVVEGEIDDLTEERIVLLETNLDDVDGETIGYTRERLF
                     SAGAVDVFITSAQGKKNRPVNVLSVITTREDYLPLVRLLMDETGTLGVRIREEPRLVA
                     DRVNEKIRVLVRNQPFTVRIKISRSLGKVIAVKPEYEDLKQIARELGIPLRHVAREVY
                     QQLPFEYMR"
     gene            complement(2275..2715)
                     /locus_tag="PKL26_04295"
     CDS             complement(2275..2715)
                     /locus_tag="PKL26_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82949.1"
                     /translation="MSIPGPYPGWPHIRERIREMIAGAEDISRITGCMLQYTDLIPVP
                     QSMDLPGMKDIEQLLSGKYNCHTVQDEVMLFRTHIPGTAGSIRSHLNRPGKPVWTLVF
                     SIQTEGPARFASCEDLLDWFDDARAGIHALFDIIVPEEIIQALK"
BASE COUNT          657 a          857 c          883 g          644 t
ORIGIN      
        1 cacgaggagt acgacacggc caaaatcatc ggcgagttcg ggctcatcct caaggatatt
       61 ggcggccctt cggtgatcgg atcgatggcc ctctcagaga ttttcgccgc ctttgaagaa
      121 gccccgatga tcggggcagg gttctcgggc ggtccggtca acccgccgct cggccacctc
      181 gaaggcgact gcgtgccggc tctccgcctc ctcatgaaac accatggcga catcttcgcg
      241 gtcgccgaag tgatccgtga ttacaagatg aactcattca tcgaccccga gatggccctc
      301 tgcggcctga acaccatctc gcggaaggcc gagcaggtga cacgcgggaa gatcacgcgg
      361 gcctgcatcc ttgcctcgga aggcgtgcgg gaccgggcta tttaccgccg ggctgcccat
      421 acctacgaaa tcatcacggg cgggaaatct gtggccgacg cgacccgcat cctcgatgag
      481 gagcgcaaga attacgtgga gcgccggggt tcggcgatcc tgtccgcatt taccggaaaa
      541 aagatcgagc tcaaattcac cgcgatcaag ccccatggac gtcgcacgga caagttcacc
      601 gaacgatact ggggctttga ctcgaatgtc tcgtacgatg tcacgattga cgggaaaaag
      661 tatcatgtcg agaacctgag cggaaaggag gtgcccgatt tcgcattgaa gggaaagaac
      721 cgcgacaacc cggactggcc ggtcgccctc ttctgcggtg cagtccttac gcaggaactc
      781 cagtacatcg gccatactat catcaacatc accgtccctg ccgcagtcgg ggcaatcctt
      841 ggaatggatg cgggcgaggc agccgacaag gcagaagacg gtgcattcct gacacgggca
      901 atcccggggg ccggagacaa ggcaaaggat gttgccaagc ttgcacagcg ggtctatgcg
      961 aagatcaatg aaccgttccc gccacaatga atgaaggcgc ttctcattga tccgaggacc
     1021 ggggggattg caggggatat gctctgtgct gccctttgcg atctcaccgg ttcggaaaaa
     1081 ccccttcagg aagttgctga tgcaatcacg ggactttccc gttgttccac cttcgaaatc
     1141 tcggttgagc ggacggtctt gccatgcagt gccgcatgta tttcggtagc catagtggag
     1201 gatgatgggc ctggcactga tgtttcacgc gaattgaacg aggttatctc caccatcggg
     1261 ctctcgccgt ccgggacggc gatggcgcat gctattctca acgatctcat ctcggtaaag
     1321 gaccggtttc acccggagac cgcaacgggc cagactttcg catctcttga tacgatcttt
     1381 gatatcctcg gccctctcgc gctccttgaa gaagcaggcc tcctggattg ccccatctat
     1441 accacccctc ccgcccttgg cggaggactg atccatactc caggcggtga cgtggggggg
     1501 cctgccccgg catcccttga gatctgcgca cgccaccgca tgccggtatc cgatagtgct
     1561 ctctccatgg aactcacgac accgaccggt gcagccctgc ttgcgaatct tgcccgcgtg
     1621 gtaaccctgt ttccggccat gacccccctg cgcaccgggt atggtgcagg tgcaggggat
     1681 catggatcca gttgccgcat cctccgggtc gtcgaggggg agattgatga tctgactgaa
     1741 gagcggatcg tcctgcttga aacgaacctc gatgacgtgg acggcgagac gatcgggtat
     1801 acgcgcgaga gactcttttc ggccggggcg gtggatgtct ttatcacgtc cgcgcagggt
     1861 aagaagaacc gccctgtcaa tgtgctgtcc gtgatcacca cccgggagga ctacctcccg
     1921 ctcgtcaggc tcctgatgga tgagaccggg acattgggtg tccggatacg cgaggagccc
     1981 cgccttgtcg ccgacagggt gaacgaaaag atcagggttt tggttcgcaa tcaacctttt
     2041 accgtgcgta taaaaatctc caggtccctc ggaaaggtca ttgcggtcaa acccgagtat
     2101 gaagatctga aacagattgc gcgggaactg ggaattccct tgcggcatgt ggcacgggaa
     2161 gtgtaccagc agctcccttt tgaatacatg aggtgaccgg cagtcaccac tgcttcgggc
     2221 ctttttcaga caaattttcc ccaaatctcc ggagaagacc cttgccggga tgcattactt
     2281 aagagcctgg atgatctctt cagggacgat gatgtcaaag agtgcgtgga tcccggcccg
     2341 ggcatcgtcg aaccagtcca acaggtcttc gcatgaggca aacctggccg gtccttcagt
     2401 ctgaatagaa aaaaccaggg tccacacagg ttttccgggc ctgttgagat gtgaacggat
     2461 tgatcccgca gtgccgggga tgtgtgtgcg aaaaagcatg acctcgtcct gtacagtgtg
     2521 acaattgtat ttcccggaga ggagctgttc gatatccttc atcccgggca ggtccatact
     2581 ctgagggaca gggatcaggt cggtatattg gagcatgcac ccggtgatcc gggagatatc
     2641 ctctgcaccg gcaatcatct ccctgatcct ctccctgatg tgcggccagc cagggtaggg
     2701 tcccgggata ctcatggaga gtgatccggc atcacgtccg agagtgcagc gacatatgac
     2761 ctcctcgacc ggtgagcggg aaaaggcgtg tggaggcgga tggtccatca agagaaggag
     2821 tatggggggg tcgtccttct atattttctg ctttcgttgc aggcattcac gattcgggag
     2881 taccagaaaa aagcgccgcc tgatgattcc ggaagtgcag tactcgcgta aatacacacc
     2941 ctcacaacgc tatttctgtc ctttatccat ggtgtcgaga agtgatttca tcaattccac
     3001 gttacgggcg acattcttgt cgatattatc cagggtgatc a
//
LOCUS       DAOQVJ010000157         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_37875_length_3026_cov_3.495456, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000157 DAOQVJ010000000
VERSION     DAOQVJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37875_length_3026_cov_3.495456"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1023
                     /gene="trpD"
                     /locus_tag="PKL26_04300"
     CDS             7..1023
                     /gene="trpD"
                     /locus_tag="PKL26_04300"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNS82950.1"
                     /translation="MMQAVLQVLSEGRLLSDPEAREAMEYIASGRATDAQMGAFLAAL
                     RIRGENAEEIATFGRVLQHYAVTIRPRVAGTLVDTCGTGGDGCRTFNISTAAAIVAAG
                     AGVVIVKHGNRSVSSSCGSADVLEALGVKIDIQPERVCRIVEETGIGFLFAPAFHPAF
                     RNAARVRKDLGFHTVFNMLGPLLNPAGAQSRLMGVYSPAFLRTLAESLQLMGAERAMV
                     VHGSGLDEITTTGPTQVAELAGGTIREYTFTPGEFGIPVSSGNALRGGTPAENAAVVL
                     RILEGGEGPGRDVVVMNAGAAIYLAGKADDLAEGITLAKESIRTGAALGKLEALVTAS
                     GRAS"
     gene            1020..1781
                     /locus_tag="PKL26_04305"
     CDS             1020..1781
                     /locus_tag="PKL26_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HNS82951.1"
                     /translation="MILDEIISSTGARVAGLPEQFPVADTGSRPKKSLFRVMGEKNGR
                     NAIISEIKYASPSRGVIRESGDPVELARMMVSAGCAALSVITEPSFFHGHPGLIPLIR
                     PHVEVPILRKDFIIDERQLAETRALGADAVLLITSVLRDRLPEFVDMAAGYSLEPLVE
                     VRTSGEVANAVDSGARLVGINNRNLADFSIDLSTTRRLAPLIRDAGCRVVSESGFVWP
                     YDVRSLRKVVDGFLIGSSVMSAKDPKKRLGGFVSA"
     gene            1778..2353
                     /locus_tag="PKL26_04310"
     CDS             1778..2353
                     /locus_tag="PKL26_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNS82952.1"
                     /translation="MKICGITSPGDAVMADQAGADAIGVVMYSDSPRSVDIETAEEIF
                     SAAGPYIARVCVSHTRSLGDIEAMLDLNPDVIQISSSLALPHPFPVRLIRVIAGGRAL
                     PIDADALLVDGSAGKGMLYDEDYASRVVKESRVPVILAGGLNPGNVALAVRRVRPYGV
                     DVATGVEDAPGVKNEQKVREFVRIAKGAVYD"
     gene            2346..>3026
                     /locus_tag="PKL26_04315"
     CDS             2346..>3026
                     /locus_tag="PKL26_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNS82953.1"
                     /translation="MIESTRFGDYGGCFVPETLMAALEELEEGFRTIVPGASFQQEMH
                     YYLHEYAGRETPLTFCRNLSRDLGCRVYLKREDLLHSGAHKLNNALGQALLAKCMGKR
                     RIIAETGAGQHGVATAIAGAALGMKVEVYMGEEDIARQALNVARMRMLGANVLPVRSG
                     SRTLKDAINEALRDWVANVRDTHYLIGSVVGPHPFPTLVRDFQTVIGRETRVQVLEKE
                     GRLPDAIVA"
BASE COUNT          592 a          846 c         1019 g          569 t
ORIGIN      
        1 aggtgcatga tgcaggcagt cctccaggtg ctgagcgagg gcaggctgct gtccgaccca
       61 gaagcacgcg aggcgatgga gtacatcgcg tccggtaggg ccacggatgc gcagatgggg
      121 gcgttccttg ccgcccttcg aataaggggc gagaatgcgg aggagattgc aaccttcgga
      181 cgggtcctcc agcattacgc ggtcacgata aggccacggg ttgcaggaac cctcgtcgat
      241 acctgcggga cgggaggtga cgggtgccgg accttcaata tcagcaccgc tgcggcaatc
      301 gtcgccgccg gtgccggcgt ggtaatcgtg aaacatggga acaggagcgt gagcagctca
      361 tgcggttcgg cggacgtgct ggaggccctc ggggtgaaga tcgacatcca gcctgagcgc
      421 gtatgcagga tcgtcgagga aacgggtatc ggttttctct ttgcgccggc attccacccg
      481 gcgttcagga atgctgcgag ggtgaggaag gatctcgggt tccacacggt attcaacatg
      541 ctcggacctc tcctcaaccc tgcaggcgca caatcgagat tgatgggggt gtactccccg
      601 gccttcttaa ggactcttgc agagtcactc caactcatgg gggccgagag ggccatggtc
      661 gtccatggtt cgggccttga cgagatcacc acgacaggcc ccacgcaggt cgccgaactt
      721 gcgggtggga ctatccgtga gtatacgttc acgcccggag agttcgggat acccgtctca
      781 tccgggaacg cccttcgggg aggcacccct gcggagaacg cggcggtcgt cctgcgcatc
      841 ctggaaggcg gggagggccc aggtcgcgac gtggtggtga tgaacgcggg agctgccatc
      901 taccttgcag ggaaggcgga tgaccttgcg gaagggatca ccctcgcaaa agagtccatc
      961 cgtaccgggg ccgcacttgg aaaactggag gcgctcgtca ccgcttccgg gagggcgtca
     1021 tgatactgga cgagatcatc tcatcaacgg gagcgcgggt cgccggcctc ccggaacaat
     1081 tcccagtggc ggacactggc agcaggccga aaaagagcct ttttagggtc atgggagaga
     1141 aaaatggacg aaatgctatc atatctgaga tcaagtatgc atcgccttcc cgcggggtaa
     1201 tccgggaaag cggagatccc gtggaactcg cccgcatgat ggtctcggca gggtgcgcgg
     1261 ccctttcggt catcaccgag ccatctttct tccacgggca tccggggctg atccccctga
     1321 tcaggccgca tgtcgaggtc ccgatcctca ggaaggattt catcattgat gagagacagc
     1381 tcgcggagac gagagctctt ggggcggacg cggtcctcct catcacatcc gtcctccggg
     1441 acaggctccc tgagttcgtg gatatggctg ccggatactc cctcgagccg cttgtggagg
     1501 tccgcacatc aggagaggtc gcaaacgcag tcgattccgg tgcaaggctt gtcgggatca
     1561 ataaccgcaa cctggctgat ttcagcatcg atctctccac cacgcggcgc ctggccccgc
     1621 tgatcagaga cgccgggtgc agggtagtct cggagagcgg ttttgtctgg ccgtacgatg
     1681 tcaggagcct tcggaaggtt gtggacgggt ttctgatcgg atcctcggtt atgtctgcaa
     1741 aagacccaaa aaagaggctg gggggattcg tatccgcgtg aagatctgcg ggatcacgtc
     1801 tcccggcgac gctgtcatgg cagaccaggc cggggctgac gctatcgggg tggtgatgta
     1861 ctcggactcc ccgcggtctg ttgacatcga gacggcggag gagatcttta gcgcggcggg
     1921 cccctacatt gccagggtgt gcgtgtcgca taccagatct cttggggaca tcgaggcgat
     1981 gcttgacctg aatcccgacg taatacagat atcgtcatca ctcgcactgc ctcacccgtt
     2041 ccctgtccgg ctcatccggg tcatcgccgg cggacgggcc cttccgatcg atgcggacgc
     2101 tctccttgtc gacgggagcg cgggaaaggg gatgttgtac gacgaggact atgcgtcccg
     2161 ggtggtgaaa gagagcaggg tcccggtcat ccttgccggg ggtctgaacc ccgggaacgt
     2221 ggcgctggca gtccggcggg tccgccccta tggcgtggac gtggccacgg gagtcgagga
     2281 cgcgcccggt gtgaagaacg aacagaaggt gcgggaattc gtgaggattg cgaaaggagc
     2341 ggtttatgat tgaatctacg agattcgggg attacggggg ttgttttgtc ccggagacac
     2401 tcatggcggc acttgaggag ctcgaggagg gtttccggac catcgtgccg ggagcgtcct
     2461 tccagcagga gatgcactat tacctgcacg aatatgcagg cagggagact cccctcactt
     2521 tctgccggaa cctctcccgg gatctcgggt gcagggtcta cctgaagcgc gaagacctcc
     2581 ttcactccgg ggcgcataag ttgaacaacg ccctcgggca ggcccttctt gcaaagtgca
     2641 tggggaagag acggattatt gcagagaccg gtgcaggcca gcacggtgtc gcgacggcga
     2701 tcgcgggggc ggcgcttgga atgaaggtgg aagtgtacat gggagaagag gacatcgccc
     2761 ggcaggccct caacgtggcc aggatgagga tgctcggcgc gaacgtgctc ccggtgaggt
     2821 ccggctcgag aactctcaag gatgccatca acgaggccct tcgcgactgg gttgcgaacg
     2881 ttcgcgacac ccactacctt atcgggtcgg tagtgggccc tcaccccttc cccacccttg
     2941 tcagggactt ccagacggtg atagggagag agacacgggt tcaggtcctc gaaaaggagg
     3001 ggaggcttcc ggatgcgatc gtcgcc
//
LOCUS       DAOQVJ010000158         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_38038_length_3017_cov_3.538150, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000158 DAOQVJ010000000
VERSION     DAOQVJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38038_length_3017_cov_3.538150"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            746..1708
                     /locus_tag="PKL26_04320"
     CDS             746..1708
                     /locus_tag="PKL26_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNS82954.1"
                     /translation="MQGGFFSEWLNRYRHYLRMRNYSPRTIQSYEQVVKHFAYYVWLR
                     RNIDPQKLMVYWEDFSKARLETSVDVEPVLLEDYLSFMTSMRTYKPKTLHRIISTLSS
                     FYRFLYSQGAVSANPMVAVERPKIKDQELRYLKHSQVLRLIDSIEDQRDRLIVRVIYA
                     TGVRVSELCEMNIEDIDFEDGTIRVRGKGGKIRIVFVDPDTLGEMDAFIEKRIEGPLF
                     VGQQGHHISPRAVQHIFRKYAPDGITPHKIRHSYASELYRRSKNLRVVQENLGHTSIK
                     TTEIYLHTDLEERKRVYQEFFPLSGRKDDEEKRGQKPRQVNEQG"
     gene            2227..2580
                     /locus_tag="PKL26_04325"
     CDS             2227..2580
                     /locus_tag="PKL26_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNS82955.1"
                     /translation="MSTILQVVEERRGVVPVLFLKGRMDATTSPEAEAHINRLIDAGE
                     HQLVVDLSGLEYISSAGLRVLLAGLKRLKQCGGTMKLSALGPEIRKVFDIAGFNRLFA
                     IHGSLDEALGAGPAT"
     gene            2620..>3017
                     /locus_tag="PKL26_04330"
     CDS             2620..>3017
                     /locus_tag="PKL26_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HNS82956.1"
                     /translation="MVSELVTASIVLFQMMCVVVVFAYLVTRTRFYSEVLEGTFSWKN
                     QAILILVFGIISIYGSESGIVILGAPLNIRDLGPMVAGFVGGPVVGLGAGLIGAGYRM
                     TMGGFTMVACSLATVLAGLLAGLINLANRRR"
BASE COUNT          696 a          819 c          812 g          690 t
ORIGIN      
        1 aacgtggtcc atacttgagc agtacatcag gcttccgaga aaaatccggg tccctttttt
       61 aagaaacaag agctgttccg gctttttatt tcccgttcca ctatataccg tttcaattca
      121 tgaatttgct tccaaagcag tgagagtcat acttccaggg cgtgatgact gtgctggtct
      181 gcaccgggcc tcacccccgt gagaagttgt ttcgaccaca gcaatcagca ggtaaggacc
      241 ccgaaaggag gggggtatct gagtgtcgtg atttggccct tgcacagggt cactccagca
      301 aattttgcgt aatcttccat gctccattct atctatcctc ctgtgaagga gaagtccaat
      361 gaccagctca ctgaaaattc catgaaatcc tggaatgaat atattcttga ttcttcctat
      421 agtcccggtt ataaacctgg aagatctcgt atacgcatta tagaggcaag aggttctcac
      481 attcgcgaaa aaatagaagg atgatacaat aatcgagtac atgctccaat cccatgcaaa
      541 tagaagtccc cgaattttgg tttttttaaa aatcctgtcc gggaccagga tccggagcgt
      601 tcaaaccctt ctctaaagag aatgatccaa aatgactgca cccgcacggg agcccccact
      661 ggtccgccag gggcgaatat ctggttgtcg cgaaatggag attctgcgca aaaagtttat
      721 aattcgagga aaaaaagaat agagcatgca ggggggattt ttctcggaat ggctcaatcg
      781 ctaccgccac tacctgcgaa tgcgcaatta ctccccccgg accatccaga gctacgagca
      841 ggtggtgaag cactttgcct actacgtgtg gctccggaga aatatcgatc cccagaagct
      901 gatggtctac tgggaggact tctcgaaggc caggcttgag accagcgtcg acgtcgagcc
      961 cgtgctcctt gaagactacc tctcattcat gacctcgatg cggacctaca agccaaagac
     1021 cctccaccgg ataatctcca ccttaagttc cttttaccgg ttcctctact cccagggagc
     1081 ggtctcggcc aacccgatgg tcgcggtcga gcggcccaag atcaaggacc aggagctccg
     1141 gtacctgaag cacagccagg tgctccgtct gattgactca atcgaggacc agagggaccg
     1201 gctgatcgtg cgggtcatct acgcgaccgg cgtccgggtc tcggaactct gcgagatgaa
     1261 catcgaggac atcgatttcg aggacgggac catcagggtg cgtgggaaag ggggcaagat
     1321 aaggatcgtg ttcgtggacc ctgatacgct aggcgagatg gatgcgttca tcgaaaagag
     1381 gatcgagggg cctctcttcg tcggccagca gggccaccat atctctccgc gtgctgtcca
     1441 gcacatcttc aggaagtatg cgcctgacgg catcactccc cacaagatcc ggcacagcta
     1501 tgcaagcgag ctctaccgca ggtcaaagaa cctgcgggtt gtccaggaga acctgggaca
     1561 cacttccatc aagaccaccg agatctacct gcacactgac ctggaggagc gaaagagggt
     1621 ctaccaggag ttcttccccc tctccgggcg aaaggatgac gaagagaagc ggggacagaa
     1681 gccccggcag gtgaacgagc agggataggg tatttccatt cgcacaaggc gccatatctt
     1741 gtgggaaaag gcgactcttc ttatctgggg gcaaagtgcc tggcgggtct tcggagcact
     1801 gtcgcttccc ggaccccttc tcggcgtaat cgatcctgcc gttccttttt ctgcgtaatt
     1861 tttccgtttt ttacaataat ccggtttcag ggcgctgttt tcctgcagcc aggtatagca
     1921 ccgaaaaacc aataaaccat atgatgctat atgtaaagag ggatggattt ttcccagaag
     1981 gttcatccgc cgaaccagtt ttcaggtcat tcccagcaac atcgaattcc tgagatagtt
     2041 gatcgtggat atttttcctt atgccaaatg agcctctatt cgaattcaaa agcgattaaa
     2101 aacgattatt tgaaggtatt ttagacccgg gaaacccctg gtttccagga gaaacagtgc
     2161 tgcctctcat ggcagaacgc agttctcgct accctcatat gggatgagat aaagagagat
     2221 ccatcaatga gcacgattct gcaggttgtc gaagagcgcc ggggtgttgt tcccgttttg
     2281 ttcctgaaag ggaggatgga tgccaccaca tcaccggagg cggaggcgca catcaaccgc
     2341 ctgatcgacg cgggagaaca ccagctggta gtcgatctct cgggcctcga gtacatcagc
     2401 agcgccggcc tgcgggtgct gcttgccggt ctgaagaggc tgaaacagtg cgggggaacc
     2461 atgaagcttt ccgcactcgg ccccgagatc cggaaggtct ttgatatcgc cgggttcaac
     2521 cgcctctttg ccattcacgg cagccttgac gaggcccttg gggcaggtcc tgcgacgtga
     2581 cctccgcacc tgtaattcgc acccctgtcc gctgatacca tggtctccga actggtcacg
     2641 gcgtccatcg tcctcttcca gatgatgtgc gtagtggtgg tctttgccta cctggtgaca
     2701 cggacccggt tctattcaga ggtgctggag gggacattct cctggaagaa ccaggccatc
     2761 ctcatcctgg tctttgggat catctccatt tacggctccg agagcgggat cgtgatcctc
     2821 ggggcacccc tcaacatccg cgacctcggt cccatggtgg ccggttttgt cgggggcccc
     2881 gtcgtggggc tcggcgcggg tttgatcggg gcaggatacc gtatgaccat gggcgggttc
     2941 acgatggtcg cctgctcgct tgcgactgtg cttgccggcc tccttgcagg gctgatcaac
     3001 cttgccaacc ggcggcg
//
LOCUS       DAOQVJ010000159         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_38200_length_3009_cov_3.728504, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000159 DAOQVJ010000000
VERSION     DAOQVJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38200_length_3009_cov_3.728504"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKL26_04335"
     CDS             complement(<1..841)
                     /locus_tag="PKL26_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNS82957.1"
                     /translation="MKKLVIKGARQHNLKNITVELPRDKFIVITGLSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGLMHKPDVDAIIGLSPAISIEQKSTSKNPRSTVGTVT
                     EIYDYLRLLFARIGIPYCPEHHIRIEAQSPERIAERIAEELGGTVTILAPVVRQKKGT
                     YQQLLKDLNREGYTRVRVNGEIHRTDEEVTLERYKKHDIDVVIDRLDASDRSRLAEAC
                     ENALKKSAGLIIVVGEDGDEHIFSSVMACPVCGLTFEELQPRMFSFNSPFGACEECNG
                     LGIR"
     gene            1131..2747
                     /locus_tag="PKL26_04340"
     CDS             1131..2747
                     /locus_tag="PKL26_04340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HNS82958.1"
                     /translation="MKSVYIIGHRQPDTDSVASVIGYAELLNLREPGRYIPAVCGPVN
                     REAGYALKKFGLEPPVYVESMEPCVGDIPSFYLQRASASMPTIDVAAMMDEQDVRNIP
                     IVDDEGHLLGLVSEHGLAKAYVTPKLDTPLTIGPVPVETIARILEARVCSAGPATIHG
                     RVYIVIDALHVALSRLASDDIAIVGDNEPTQLALLSAGIAVLVVAEGAPVGERVLEAA
                     RRKGATILSTPLDAFSVGRMLHLSLPAGKVVATDVPVIRLEDSLAYARKMVTDSKYRT
                     ACVVDENRALLGMISRNTFLDDVQKQVILLDHNEYAQAVEGVESAEILEVIDHHRLGA
                     ITTLKPVRFQNEPVGSTSTIITRKFMESGTIPSPGTAGMLLAGILSDTLILKMSTTTP
                     EDVSAVEFLSGVTGIDVQQFGADLLQHGINLDSTPLYQLLSQDVKRYTLFGREVIIAQ
                     VMTASRKYAEEHGKEIQAELENLRRGNSVDLYIVLFTDIIGNQSDLFVSADHATQNVL
                     GYGTQPVLLPGVMSRKKDFLPVFGGKLRDL"
     gene            complement(2770..>3009)
                     /locus_tag="PKL26_04345"
     CDS             complement(2770..>3009)
                     /locus_tag="PKL26_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82959.1"
                     /translation="GEVQNEEHRLCEALKSHTVKKYFHIPTEDEVSSDQELPSDACNG
                     VPERIPCPVCEGAGTIAFDEDEWELKVVDDEDETG"
BASE COUNT          601 a          880 c          896 g          632 t
ORIGIN      
        1 tccgtatccc gagcccgttg cactcctcgc aggcgccgaa ggggctgttg aaggaaaaca
       61 tgcggggctg tagttcctcg aacgtgagcc cgcagaccgg gcaggccatc acggaagaga
      121 agatatgctc gtccccgtcc tcccccacga cgatgatcag cccggctgat ttcttgagcg
      181 cgttctcaca ggcctcggcc agccgggagc ggtccgatgc gtccaggcgg tcgataacca
      241 cgtcgatgtc gtgtttttta taccgctcga gcgtgacctc ctcgtcggtg cggtggatct
      301 ccccgttaac ccggaccctg gtgtagccct cgcggttgag gtcttttaag agctgttggt
      361 acgtcccttt cttctgccgg accaccggcg cgaggatggt caccgtgccc cccagttctt
      421 ctgcaatccg ctctgcaatc cgttccgggg actgtgcctc gatgcggatg tgatgctcag
      481 ggcagtaggg tatgcctatc cttgcaaaga ggaggcggag gtaatcgtag atctcggtga
      541 ccgttcccac cgtgctcctg gggttcttgc tggtgctctt ctgctcgatg gagatcgcgg
      601 gggagaggcc gatgatggcg tccacgtccg gcttgtgcat caggcccagg aactggcgcg
      661 catacgcgga aagtgactcg acgtaccggc gctgcccctc ggcatagatg gtgtcgaacg
      721 cgagcgtcga cttccccgag cccgagaggc ccgtgatgac gatgaacttg tcgcgcggca
      781 gctcaacagt gatattcttg aggttgtgct ggcgtgcgcc cttgatgacg agcttcttca
      841 ttcgttctgt cgcgtgtatg atgtggatgt gtatccatat aggggtgtcc gttggggtag
      901 gagtgggaag tggcaggagt atgcagaacg atgaaaaaag gtgcatgaac tgtgcgaacg
      961 cagaaacgtc acatccactc cgtagactaa tacgccccac gtgtgggatt tcccggccag
     1021 gacgtgaact tatataccgc ccggcgactc cattattgga ctgtgaaagg agacccgact
     1081 gttcccggga ttccctcaaa aataccatct cctgccagga agagaccagc atgaagagcg
     1141 tctacatcat cgggcaccga cagccggata ccgacagcgt ggcgagcgtc atcgggtatg
     1201 ccgagttact gaacctgcgc gaaccgggcc gttatatccc cgcggtctgc ggtcccgtga
     1261 acagagaagc cggttatgcc ctgaaaaagt tcggactcga gccgccggta tacgtcgaga
     1321 gcatggaacc ctgtgttgga gacattccct cgttctatct gcagagggca tctgcatcaa
     1381 tgccgaccat cgacgtagca gccatgatgg acgagcagga tgtcaggaac atcccgatcg
     1441 tggacgatga ggggcacctg ttggggctcg tatcggagca cggcctcgca aaggcgtacg
     1501 tgacgccgaa acttgatacg ccactcacca tcggccctgt ccccgtggag accatcgccc
     1561 ggatacttga agcccgggtc tgcagcgcgg gtcccgctac catccacggc agggtctaca
     1621 tcgtcatcga cgccctccac gtggccctct cccggctggc ttcagatgat atcgcgattg
     1681 tgggggacaa cgagccgacc cagcttgcac ttctctctgc aggcattgcg gtacttgttg
     1741 tcgcggaggg tgcgcctgtg ggggagcgtg tgctcgaagc cgcaaggaga aagggggcca
     1801 caatcctctc cactcctctc gatgcgtttt cagtcggcag gatgctccac ctctcgctgc
     1861 ccgccgggaa agtggtggca accgacgtcc ccgtgataag gctcgaagat tccctcgcct
     1921 atgccaggaa gatggtgacc gactccaagt accgcactgc ctgcgtggtg gacgagaacc
     1981 gggccctgct tgggatgatc tcgaggaaca cgttccttga cgatgtccag aagcaggtca
     2041 tcctcctcga tcacaacgag tatgcgcagg cggtcgaggg agtggagtcc gcggagatcc
     2101 tcgaggtgat cgaccaccac aggctcgggg ccatcactac gctcaagcct gtccggttcc
     2161 agaacgagcc ggtggggtcg acctcgacca tcatcacccg caagttcatg gagtcgggca
     2221 caatcccttc acctggcacg gcaggaatgc tccttgcagg gatactctcc gacaccctca
     2281 tactcaaaat gtcgactacc acccccgagg atgtgtccgc tgtggaattc ctctccggtg
     2341 tgacagggat tgatgtgcag cagtttggtg ccgatctcct ccagcacggg ataaacctcg
     2401 acagtacgcc tctgtatcag ctcctgtcgc aggacgtcaa gaggtatacc ctctttgggc
     2461 gggaggtcat catcgcccag gtgatgacgg catcgcggaa gtatgcggag gaacacggta
     2521 aagaaatcca ggcggaactg gagaatctcc ggagagggaa ctccgtcgac ctgtatatag
     2581 tgttgttcac cgacatcatc ggaaaccaga gcgacctctt tgtatccgcg gatcacgcga
     2641 cacaaaacgt ccttgggtat gggacgcagc cggtcctcct tccgggcgtc atgtcaagga
     2701 agaaggactt tctcccggtg ttcgggggca agctgcggga tttataaaaa aaagtgtgac
     2761 cgggtattat catccggtct cgtcttcgtc atcgacgacc ttcagttccc attcgtcctc
     2821 gtcaaatgcg atggtcccgg caccctcgca caccgggcag gggattcttt caggcacccc
     2881 gttgcaggca tcagaaggga gttcctgatc ggagctcacc tcgtcctcgg tagggatgtg
     2941 gaaatatttc ttcaccgtat gggatttcag cgcctcgcag agcctgtgct cctcgttctg
     3001 gacctcgcc
//
LOCUS       DAOQVJ010000160         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_38276_length_3005_cov_3.793898, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000160 DAOQVJ010000000
VERSION     DAOQVJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38276_length_3005_cov_3.793898"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1301)
                     /locus_tag="PKL26_04350"
     CDS             complement(66..1301)
                     /locus_tag="PKL26_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS82960.1"
                     /translation="MKYSHVCAIIGVILCAALIAGCITPTQHQGTPTPTVSPSGGDVI
                     VTYTKGTGPMPTLLGTDQIDGYIAWQPFVEVPPMAGIGKILLYSGDLPPQGKWKDHPC
                     CVFTANQKVIAQNPDLTNALTASMILATQYLEEHPEESAEIVADWLAGKGNFTYGEIS
                     VSSVDVLKRAFPTVKFVNDPTDKWMDSNIEFVHALRELEVLTGSLKASSDADSKTILF
                     DTAPYNAAQSMIGAGKITTPTPVKGSVGIGYLMSDHHAALFVAIKKWEYFNDTYGIAI
                     KPRDTAASRPDIADFIVNGEKVAEIKLISGDAGPQLMQLMATDNIQYALVGNPPAISA
                     ADKGTPVKIIMSINSEGSGVVVTEDSPANDWNSFVAWAKERSAAGKPLRIAAPGKGSI
                     QDVMIRYSLETSGLSIKEG"
     gene            1491..2021
                     /locus_tag="PKL26_04355"
     CDS             1491..2021
                     /locus_tag="PKL26_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS82961.1"
                     /translation="MQRDPVDRLVNAALISDDEFVHALREVMQRDLRISVRELADKSG
                     VAPSSLYKMMNGKRSPNLTTLRAIVQAMRRITLSSEGEFIGLIAARPVLDVILERNAE
                     VDGRKVRIREYPVHTMEEAIVAAVRAEREGAVAIVCAPIVSSVIEQLVKVPVATIIPR
                     ESVQRAIELAARKAWL"
     gene            2065..>3005
                     /gene="argH"
                     /locus_tag="PKL26_04360"
     CDS             2065..>3005
                     /gene="argH"
                     /locus_tag="PKL26_04360"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNS82962.1"
                     /translation="MEQDVVRRGRLTGERSGEMMHFLSSMGADRCIADADILVDIAHV
                     LMLSRQKIIGKEQAGRLLEALLELYENGIPEAAFDDRFEDIHAGIETLLIATIGEDAG
                     GRLHIGRSRNDEVATCIRLRVRDDLVRQLSILIRMREVLLDLAGEHIESVMPGFTHLQ
                     HAQPTTLAHHLLAHEAAFARDYQRLSGALSRVDWCPLGSAAFASTSYPVQREFVAGLL
                     GFQGVLENTMDAVSSRDFALEVLSCLSIMMTHASRVCEELVVWSSGFARFVELDDAFC
                     STSSIMPQKKNPDTAEVMRAKAASVLGSATASFAIVKG"
BASE COUNT          635 a          821 c          886 g          663 t
ORIGIN      
        1 acctcccacc caacgacaag gagaatgggg aggaggaacc ggagatacct cattgtcccc
       61 taaggttatc cttcctttat tgataaacca cttgtttcga gggagtaccg gatcatgacg
      121 tcctgtattg atccctttcc gggagccgcg atcctgagcg gctttccggc cgcagagcgc
      181 tcctttgccc atgcaacgaa gctgttccag tcattggcag gagagtcctc ggtcaccacg
      241 actccggagc cctctgaatt gatggacatg atgatcttga caggggtgcc cttgtcggcc
      301 gcggagattg caggcgggtt tccgaccagt gcatactgga tattatcagt tgccatcaac
      361 tgcatcagct gcgggcctgc atcacccgat atcagcttga tctcagcaac tttttctccg
      421 ttgactatga agtcggcgat atccggcctg gatgcggcgg tatccctggg tttgatggcg
      481 atgccgtatg tatcgttgaa gtactcccac ttcttgatgg ccacgaacag ggccgcgtgg
      541 tggtcagaca tcaggtaacc gattccaacc gatcccttca cgggtgtggg tgtggtaatc
      601 ttcccggcgc cgatcatgga ctgtgccgca ttgtatggcg ccgtgtcaaa gagaatggtc
      661 tttgaatcag cgtcagagga ggccttcagg gaaccggtga gaacctccag ctctctcaat
      721 gcatggacga attcgatatt tgagtccatc cacttgtcgg tcgggtcatt cacgaacttc
      781 accgtgggga aggcgcgctt cagcacgtct accgatgaga ccgagatctc tccgtaggta
      841 aagttgcctt tccctgcaag ccagtccgcc acgatctcgg cactctcctc ggggtgctct
      901 tcaaggtact gggtggcgag gatcatcgat gcagtcaggg cattggtgag gtcaggattt
      961 tgggcgatca ccttctggtt ggcggtgaat acacagcatg ggtggtcctt ccacttgccc
     1021 tggggaggca ggtcgccgga atacagaagg atctttccga tccctgccat gggcgggacc
     1081 tcgacaaagg gctgccaggc gatatagccg tcaatctggt ccgtcccaag cagagtgggc
     1141 ataggcccgg tccccttggt gtaggtgacg attacatcgc cacctgaagg ggaaacggtg
     1201 ggggtgggag tcccctggtg ctgggtaggt gtgatgcagc ctgcgatcag ggccgcacag
     1261 agtatgacac cgatgattgc acacacatgc gaatacttca tgcacacact gttctcatat
     1321 gcgcaaataa attctccgaa agatgactat ttattgtcca agtgtaacca aaatttgaaa
     1381 ttaatagtat tattgtgtta aatattgtga caaagggaga tcatatccag tgcgaatgga
     1441 caaaaaggtt ataccccgtg aactcccaag ctcacgttcc aggaactccc atgcaaagag
     1501 acccggtcga caggctcgtg aatgcagcac tcatctctga cgatgaattc gtgcatgccc
     1561 tccgcgaggt catgcagcgt gacttgagga taagcgtcag ggagcttgcc gacaagagcg
     1621 gtgtcgcccc gagctccctc tataaaatga tgaatggaaa gaggtctccg aatctcacca
     1681 cgctgcgtgc gatcgtccag gcaatgagga ggatcaccct ctcttccgag ggggagttca
     1741 tcggcctgat cgcggcacgg ccagttctcg acgtgatact ggagcggaat gccgaggttg
     1801 acggccggaa ggtaagaatc cgcgagtatc cggtccacac aatggaagag gccatcgtcg
     1861 ccgcggtcag ggccgaacgg gagggggcgg tagccattgt ctgcgcaccc atcgtcagca
     1921 gcgtcatcga gcagctggtg aaggtaccgg tcgccacgat catccctcgt gaatcagtcc
     1981 agagggcaat tgaacttgcg gcccggaaag cgtggcttta atctcattcc agcgtcctag
     2041 ttatacgcga ggttatggtt taggatggag caggacgtgg tgcgcagggg acggctcacc
     2101 ggtgagcgtt caggagagat gatgcatttt ctctcttcaa tgggtgcgga tcgctgcatc
     2161 gcagatgcag atatcctggt ggacatcgcc catgtgctga tgctctcccg gcagaagatc
     2221 atcggaaagg agcaggcggg aagactcctg gaagcactcc tggagctgta tgaaaatggg
     2281 atccctgaag cggccttcga cgaccggttc gaggatatcc atgcgggcat cgagaccctc
     2341 ctcatcgcca ctatcggcga ggatgcaggg ggaaggctgc acatagggcg atcgaggaac
     2401 gacgaggtgg cgacctgcat ccggcttcgc gtgagggatg acctcgtacg ccagctctcc
     2461 atcctcatcc gtatgaggga ggtgctgctt gaccttgccg gggagcacat agagtctgtc
     2521 atgcctgggt tcacccacct tcagcacgcc cagcctacca cgcttgcgca ccaccttctc
     2581 gcccacgagg cagcgtttgc aagggattac cagaggcttt ccggggcgct ctcacgggtt
     2641 gactggtgcc cgctcggttc cgccgctttt gcctccacga gttacccggt ccagagagaa
     2701 tttgtcgccg ggctcctcgg tttccaggga gtgctcgaga ataccatgga tgccgtttcc
     2761 tcgcgcgact ttgccctgga ggtactctcc tgcctctcta tcatgatgac ccatgcaagc
     2821 cgggtctgcg aggagctggt ggtgtggagc tcaggctttg ccaggttcgt ggagctcgat
     2881 gacgccttct gctccacgag ctccatcatg ccgcagaaaa agaaccccga tacggcagag
     2941 gtgatgcggg ccaaggccgc atcggtcctt ggctctgcca ctgcatcctt cgcaatcgtc
     3001 aaggg
//
LOCUS       DAOQVJ010000161         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_38355_length_3001_cov_4.350305, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000161 DAOQVJ010000000
VERSION     DAOQVJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38355_length_3001_cov_4.350305"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..494)
                     /locus_tag="PKL26_04365"
     CDS             complement(48..494)
                     /locus_tag="PKL26_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HNS82963.1"
                     /translation="MAEEKIRRIRARAEKSARFRRDGEGKKERLADTWRRPRGLHNKQ
                     RIQKKAKGPMPTPGYGSPADVRGLHPSGYRDVLVHTAGELEGLDPALDAIRIAAQVGA
                     RKRGLIQEMALAAGLKVLNPKDLSAKKEAEPAGEEPENDEEVEEND"
     gene            complement(500..1024)
                     /locus_tag="PKL26_04370"
     CDS             complement(500..1024)
                     /locus_tag="PKL26_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNS82964.1"
                     /translation="MATERTVRIPKGVKVEIDGPLVRVKGQKGQLERNFRFPQIAMKV
                     EGDEVVISTGADRKRVVAMVGTIQAHVANMCKGVSEGFEYRMKVVYSHFPIQLKLSGN
                     RLEINNFLGEKKPRYALIRDGVTAKVGNDEIVLSGINRETLGITSANIEDATRIRNRD
                     PRIFQDGIYRIQRG"
     gene            complement(1043..1435)
                     /locus_tag="PKL26_04375"
     CDS             complement(1043..1435)
                     /locus_tag="PKL26_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNS82965.1"
                     /translation="MARLNTIADGMCALKNAADGGKPAVIIEPASKLLGAMFRIMQEA
                     GYINGFEFIEDGRGGQFMVHLNGRINRCGAISPRYPVKISDLEYWETQFLPGKNFGIL
                     LLSTSKGVITHEQARREGVGGELLGYVY"
     gene            complement(1452..1607)
                     /locus_tag="PKL26_04380"
     CDS             complement(1452..1607)
                     /locus_tag="PKL26_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNS82966.1"
                     /translation="MGGEKTKKYGRGANECKMCGRKQALVRRYNIMFCRQCFREWAQK
                     MGFKKMN"
     gene            complement(1609..2115)
                     /locus_tag="PKL26_04385"
     CDS             complement(1609..2115)
                     /locus_tag="PKL26_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNS82967.1"
                     /translation="MNPMRDVFVEKVVVHMGVGEAGDKLTKAVDLMKTITGGTPVRSN
                     AKQTNTTFGIRKGAPISCRVTLRGEKATNFLNTALGIVDRRISGSQIDRRGNFSFGVE
                     EHTDFPGMSYDPQIGIYGMDINVVLARRGIRISRRAMEQRKLPQKQQVGQEDAIAFLK
                     SGHQVEVL"
     gene            complement(2112..2837)
                     /locus_tag="PKL26_04390"
     CDS             complement(2112..2837)
                     /locus_tag="PKL26_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HNS82968.1"
                     /translation="MSDHLKRLNAPKSWHIARKTEKFIARTSPGPHNANAMPVAVWLR
                     DHMRFASNMKEVKQILVQKDVIINGRPCRNPRMGIGIFDIISVPRINKHYRILRDKRG
                     RHKTIEIDAESAKSRLAKIRNKTIVPGGKVQLNLRFGANVLADNTYKPGDSVVISLEP
                     ENRFAILDHFPFAVGNMAMVIGGKHSGIVARIVGIEKVPGSVPNRVLLEDPESKVRFE
                     TIDEYIYMVGREQPALTEWGIEE"
     gene            complement(2837..>3001)
                     /locus_tag="PKL26_04395"
     CDS             complement(2837..>3001)
                     /locus_tag="PKL26_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal protein L26"
                     /protein_id="HNS82969.1"
                     /translation="DEVDTRKSRIVVQGVSIPKSDGTEMPRPVSPSNVQITKLNLKDP
                     RRAASLGESE"
BASE COUNT          465 a          951 c          851 g          734 t
ORIGIN      
        1 caccgcactt cagaattgag gcggataacc tcttctgggt ggagagatca gtcattctct
       61 tccacctcct cgtcgttctc aggctcctct cccgcgggtt ctgcttcctt ctttgcggac
      121 agatccttcg ggttgaggac cttgagtcct gcggcgaggg ccatctcctg aatcaggcct
      181 ctcttccttg cgccgacctg ggccgcgatg cggattgcat cgagcgcagg atcgagtccc
      241 tcgagttcgc cggcagtgtg gaccagcacg tcgcggtatc cgcttgggtg cagtcccctg
      301 acgtcggccg ggcttccgta gccgggcgtg ggcatcggcc cctttgcctt cttctggatc
      361 cgctgcttgt tgtgaagccc gcggggcctc ctccaggtgt cggcgagccg ctccttcttt
      421 ccctccccgt ctctcctgaa acgggcggat ttctctgccc tggcacgaat acgtctgatt
      481 ttttcttcag ccatggtctt catcccctct gtatccggta aatgccgtcc tggaagatgc
      541 ggggatcgcg gttccggatc cttgtcgcgt cctcgatgtt cgcagaggtg atgccgagcg
      601 tttcgcggtt gatcccggag aggacgatct cgtcgttgcc caccttcgcg gtgactccgt
      661 ccctgatcag ggcataccgc ggcttcttct cgccaaggaa gttattgatc tcgagcctgt
      721 ttcccgaaag cttgagctgg atcggaaagt ggctgtagac cactttcatg cggtactcga
      781 acccctcgga tacccccttg cacatgtttg caacgtgcgc ctggatggtt cccaccatgg
      841 cgacgaccct ttttcggtcc gcgccggtgg agatgacgac ttcatctcct tcgaccttca
      901 tcgcgatctg ggggaaccgg aagttcctct cgagctgccc cttctggcct ttcaccctca
      961 caaggggccc gtcaatctct accttcacac cctttgggat gcgcactgtt cgttctgttg
     1021 ccattgtatt cacgcacccc ctctagtaga cataccccag aagctccccg cccacaccct
     1081 cgcggcgggc ctgttcgtgg gtgatgaccc ccttcgaggt ggagaggagg agtatgccaa
     1141 agttcttccc cggaaggaac tgggtctccc agtactccag gtcggagatc ttcaccgggt
     1201 aacgggggga gatggcgccg caccggttga tcctcccatt gaggtgaacc atgaactggc
     1261 ctccccgccc gtcctcgatg aactcgaagc cgttgatgta cccggcctcc tgcatgatgc
     1321 ggaacatcgc gccgagcagt ttgctcgccg gctcgatgat gaccgcgggc ttgcctccgt
     1381 ctgccgcgtt cttgagggcg cacatgccat ctgcgattgt attaagtctt gccataagcc
     1441 tgacgctccc tctagttcat ctttttaaag cccatcttct gtgcccactc gcggaagcac
     1501 tggcggcaga acatgatgtt gtagcgccgc accagggcct gtttccggcc gcacatcttg
     1561 cactcgtttg caccgcggcc gtatttcttg gtcttttctc cgcccatttc agagcacctc
     1621 cacctggtgg cctgacttga ggaatgcaat tgcatcctcc tggcctacct gctgtttctg
     1681 gggaagcttg cgctgctcca tcgctctccg ggatatgcgg attccccggc gggcaaggac
     1741 gacattgatg tccatgccgt agatgccgat ctgggggtcg tagctcatcc ctgggaaatc
     1801 ggtgtgctcc tcgacgccga acgagaagtt cccgcggcga tcgatctggc tcccgctgat
     1861 ccgcctgtcc acgatgccga gcgccgtgtt gaggaagttc gtcgcctttt cgccgcgaag
     1921 ggtgacccgg cagctgatag gggcgccttt ccggatgccg aacgtagtgt tggtctgctt
     1981 ggcattgctg cgcaccggcg tgcctcccgt gatggtcttc atcaggtcca cggcctttgt
     2041 cagcttgtca ccggcctcgc cgacccccat gtgcaccacg accttctcca caaagacatc
     2101 gcgcattggg ttcattcttc aatcccccac tcggtgaggg ccggctgttc gcggcccacc
     2161 atgtagatgt actcgtcgat ggtctcgaag cgcaccttgc tctcggggtc ctccaggagc
     2221 acacggttcg gcacgctccc cggcaccttc tctatcccca cgatgcgggc gacaatgccc
     2281 gaatgctttc ccccgatgac catggccatg ttgccgacag cgaacgggaa gtggtcgagg
     2341 attgcgaacc ggttctcggg ttcaagggag atgaccacgg agtccccggg cttgtaggtg
     2401 ttgtctgcaa gcacgtttgc gccgaaccgc aggttgagct gcacctttcc acccgggaca
     2461 atcgtcttgt tccggatctt tgccaggcgg ctctttgccg actcggcatc gatctcgatg
     2521 gtcttgtgcc gccccctctt gtcacgcagg atgcggtaat gcttgttgat cctggggaca
     2581 gagatgatgt cgaagatccc gattcccatc ctggggttcc ggcatggcct gccgttgatg
     2641 atgacatcct tctggaccag gatctgcttg acctccttca tgttggaggc aaagcgcatg
     2701 tggtcgcgga gccacaccgc caccggcatg gcgttggcgt tgtgcggtcc gggggaggtc
     2761 ctggcgatga acttctccgt ctttctggca atgtgccagg acttgggggc attgagcctc
     2821 tttaggtggt ctgacattat tcactctccc ccagggacgc cgctctccgc ggatccttca
     2881 ggttcagttt ggtgatctgc acgttggaag gcgagacggg tcttggcatc tccgtcccgt
     2941 ccgacttggg aattgagaca ccctggacga cgatgcggga cttccttgtg tccacttcgt
     3001 c
//
LOCUS       DAOQVJ010000162         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_38544_length_2991_cov_3.783719, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000162 DAOQVJ010000000
VERSION     DAOQVJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38544_length_2991_cov_3.783719"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKL26_04400"
     CDS             complement(<1..820)
                     /locus_tag="PKL26_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNS82970.1"
                     /translation="MREPPVKKVLYWCPRCNIPLLGRSCMCGAEGRAIPLLRPYDARP
                     ALKADRDLLAWLLDERYGTDTLPRVIVLNKTGGIDRKDLVIANGGRFGWLSYDPAQQR
                     FDLELTFESLPFILPSARRNVLELAQLQGAARDVESGRRIGGKRYPAEGKVPDGGVLV
                     RWNGHGGVGVRTGGMVKVKEVGRVSPGEYPDPGWDEVISRNRLHLKNLERNAIRFIRQ
                     QAEGHECVNISFSGGKDSTAALELARRAGFEDSYFVDTGMEFPETLGFVKSLGIR"
     gene            949..2433
                     /gene="purH"
                     /locus_tag="PKL26_04405"
     CDS             949..2433
                     /gene="purH"
                     /locus_tag="PKL26_04405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNS82971.1"
                     /translation="MKWALLSVWNKEGIVDLARELHRHRLQLLSSGGTGAALKEASIP
                     FTEVSAYTGSSEMMDGRVKTLHPRIHGGLLGRRGIDDGVMREEGIHQIDLLVANLYPF
                     EEMANKGLPLGELVEYIDIGGPAMIRAAAKNHKDVAVVTAPSDYPLVKEALESGGFTA
                     DQRLLFAKRAFARTAAYDAAISNYLHHLGSEFPDVYTVQFRNGRTLRYGENPHQRGAV
                     YGQAGVAGSEPLQGKQMSYNNYLDLSSAAGLVREFSEPAAVIVKHNNPCGVAVGKNLA
                     DAYIQARDVDPVSAYGSVVALNREVDRHLAEDIASTFVEVVAAPEYTRNALQVFGKKE
                     NLRVLHLPAAERGWELRSIDGGLLVQDTPPYQERWEVVTDRDATPAEMKAMKFACIVC
                     AHTRSNAIVFADATRTLGIGAGQMSRVDAAKIAIEKSRFSLAGSAVASDAFLPFPDTL
                     EVAAAAGATALVQPGGSIRDKEVIEAANRLDVAMVFTGVRHFRH"
     gene            2411..>2991
                     /locus_tag="PKL26_04410"
     CDS             2411..>2991
                     /locus_tag="PKL26_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNS82972.1"
                     /translation="MSGISGTERDRLLTLFFGYLLISGDPTLLSRISVIHMDYGKMIR
                     DSFEYAKDGLTKNPKTWIMLIVLTLLPVIPFILLVLLMGASLMAYTETDIPASLIATL
                     IMGFVIAFVMALILGAFSMGYLLKILRGEVPLPEVTGFGTMFVDGIKLLVIEIIYLIP
                     VLIILAVTAGAALISALPILMSMPAEPDFEALMP"
BASE COUNT          601 a          899 c          846 g          645 t
ORIGIN      
        1 tcctgatgcc gagacttttt acgaaaccga gggtctcggg aaactccatg ccggtgtcga
       61 cgaagtagct gtcttcgaac ccagccctcc gggcgagttc cagggccgcg gtgctgtctt
      121 ttcctcccga gaacgagata ttcacgcatt catggccttc tgcctgctgc ctgatgaagc
      181 gaatggcatt ccgctcgagg ttcttcaggt gcagcctgtt ccgggagatt acctcgtccc
      241 agccgggatc cgggtattcc ccgggtgaga cccggccgac ctcctttact ttcaccattc
      301 cccctgtgcg cactcccact ccgccatgcc cattccaccg gacgagcacc cccccatccg
      361 gaacctttcc ctctgctgga taacgcttcc cacctatccg ccttcctgac tcaacatccc
      421 tggcggcacc ctgcagttgc gcgagctcaa ggacatttct ccttgcagac ggcaggatga
      481 acggcaggga ttcgaacgtg agctcgagat cgaacctttg ttgcgccggg tcgtacgaga
      541 gccacccgaa ccgtcctccg ttggcgatca ccaggtcctt cctgtcaatg cctcccgtct
      601 tgttgagaac gatgacgcgt gggagtgtgt cggttccata ccgctcgtcc aacagccagg
      661 ccaggaggtc ccggtccgct ttgagcgcgg ggcgggcgtc ataggggcga aggagcggga
      721 tagcgcggcc ttccgcaccg cacatgcagc tccttccaag gagcgggata ttgcaccgcg
      781 ggcaccagta cagcaccttc ttgacggggg gttctctcat cctgcagata ggtatcatgg
      841 cgtgacagga taatgcttga ccccacgaaa ggggggtgac gaccgccgtc tccttttcca
      901 tgatcccttt catcactctc ttatcccatt atctcccaat agatgaccat gaagtgggca
      961 ctgctctctg tgtggaacaa ggagggtatc gtcgacctcg cccgagaact gcaccgccac
     1021 cggttgcagc tgctcagctc gggagggacc ggcgcagcat tgaaggaggc ttccatcccc
     1081 ttcacagaag tatcggcgta taccggctcg tccgagatga tggacggccg cgtcaagaca
     1141 ctccaccccc gcatccacgg tggccttctt ggcaggaggg ggatcgatga cggagtgatg
     1201 cgggaggagg ggatccacca gatcgacctt ctcgtcgcga acctctaccc cttcgaagag
     1261 atggcgaata agggcctccc gcttggggag ctcgtcgagt atatcgatat cggagggccc
     1321 gcaatgatcc gagctgctgc caagaaccat aaagacgtgg cagtggtcac ggctccctcg
     1381 gattacccgc ttgttaaaga agcgcttgaa tcaggagggt ttacggcaga tcagcgcctg
     1441 ctgttcgcaa aacgggcatt tgcccggaca gcggcatacg atgcggcgat cagcaattac
     1501 ctccaccacc tcgggtcgga attccccgac gtctacaccg tccagttcag gaacgggagg
     1561 acgcttcggt atggagaaaa cccgcaccag cgcggtgccg tatacggcca ggccggtgtc
     1621 gcggggagtg agcccctgca gggaaagcag atgtcctaca acaactatct tgacctctcg
     1681 tccgctgcag gccttgtgcg ggagttttcc gagcccgcag cagtgattgt caagcacaac
     1741 aatccatgcg gcgttgcagt cggaaagaac ctggccgatg cctatatcca ggcccgcgat
     1801 gtggacccgg tctcggcata tggatcggtc gtcgctctga acagggaggt ggatcgtcac
     1861 ctcgcagagg atattgcgag cacctttgtc gaggtcgtgg cggcccccga gtatacccgc
     1921 aacgccctgc aggtatttgg aaagaaggag aacctccgtg tgttacacct ccctgccgct
     1981 gaaagaggct gggaactgcg gagcatcgat ggaggcctgc tcgtgcagga cacccctccc
     2041 taccaggagc gatgggaggt cgtcaccgac cgggacgcca ccccggcgga gatgaaggcg
     2101 atgaagtttg cctgcattgt atgcgctcat acccggagca atgccatcgt ctttgcggat
     2161 gcaacgagga cgctcgggat cggggcaggg cagatgagcc gcgtggacgc ggcaaagatc
     2221 gccatcgaaa aatcccggtt ctcccttgcg ggatccgcgg tcgcttcgga cgctttcctg
     2281 ccattccctg ataccctgga ggttgctgcc gctgccgggg ccactgctct tgtccagccc
     2341 ggcggatcaa tccgggacaa agaggtcatc gaggccgcaa accgcctgga tgtcgcgatg
     2401 gtctttactg gtgtccggca tttccggcac tgaaagggac cgcctgctca cccttttttt
     2461 cggataccta ttaataagcg gagatcccac cctgctctcc agaatatcgg tgattcatat
     2521 ggattacgga aaaatgattc gcgattcgtt tgagtatgca aaggatgggc ttacaaaaaa
     2581 cccaaagacc tggataatgc tgatagtgct gaccctcctt ccagtcattc cattcattct
     2641 cttagtgtta ctcatggggg cttcattgat ggcgtacact gagacggaca ttcctgcctc
     2701 gctcatcgcg acgttgatca tggggtttgt cattgcattc gtgatggccc tcatacttgg
     2761 tgccttttcc atgggatacc tgctcaagat cctcaggggc gaagtcccgc tacccgaagt
     2821 gaccggtttt ggcacgatgt ttgttgatgg gatcaagctc ctggtaatag agataatcta
     2881 tctcataccc gtgttgatca tcctcgctgt cactgcaggt gccgcactca tcagcgcgtt
     2941 acctatcctc atgtcaatgc cggccgaacc tgactttgaa gcactgatgc c
//
LOCUS       DAOQVJ010000163         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_38892_length_2972_cov_4.548852, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000163 DAOQVJ010000000
VERSION     DAOQVJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38892_length_2972_cov_4.548852"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKL26_04415"
     CDS             complement(<1..322)
                     /locus_tag="PKL26_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNS82973.1"
                     /translation="MHRTAIPMLGVLLVISLLVAGCTSPEHPGPPVVGETHAPQSTII
                     RSSPPLDAVVDANNRFAFDLYNTLADNPEYGDGNIFFSPFSLSTALAITYEGARGQTA
                     DEIQS"
     gene            complement(416..1741)
                     /locus_tag="PKL26_04420"
     CDS             complement(416..1741)
                     /locus_tag="PKL26_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNS82974.1"
                     /translation="MNPSLAAIPALAAVALLAVILAGCTGPGEPGPSIEVTPTPNVTV
                     TTSPSPPNPALPGSATSVVDANNRFALDLYGRIADDPKAGDGNIFFSPFSISSALAIT
                     YEGARGTTAEEIRSVFHFPENQTVMRSGFAGIISGINSKANAYMLRTANALWAEQTYP
                     FLAGYISAAEQHYGAKTTNLDFINKPEESRVTINTWVEDLTEDRIKDLLQPGTIDPLT
                     RLVITNAIYFKGTWVKQFDENKTTDADFRTGTGKTVRVRMMERTDEDAIYGYAETDIL
                     QALSMPYESGDGKDLSMLVLLPKTDDLSMVEESLNTSTLSLLHDLLKTRRVIVYFPKF
                     VMETKYSLPGMLSAMGMPTAFTRAADFSGMDGTKDLYIDDVIHQAFVEVNEEGTEAAA
                     ATAVVMRLSAMPLEETVPLFRADHPFIFLIQENETGNILFMGKVSDPSG"
     gene            1888..2685
                     /locus_tag="PKL26_04425"
     CDS             1888..2685
                     /locus_tag="PKL26_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS82975.1"
                     /translation="MERYTWNPADYAEHSHAQYLWAGEVIAKLDLKGTESVLDIGCGD
                     GKVTALLASHLPSGEVIGIDSSAGMVNCARESFPGSDYSNISFLCMDAMDIRFSERFD
                     LVFSNATLHWVQDHATMLQGVYRCLKNRGRIFFQMGGRGNAREILDLAGSLIHSGRWR
                     RYFSDFRSPYTFLSAEEYQVLLRDAGFLPIRVELIPKDMTQVGCDGLCGWIRTTWLPY
                     TERIPEQQKNRFISDLAESYLEKYPPGSEGLIHVGMVRLEVEAQKPG"
     gene            complement(2745..2927)
                     /locus_tag="PKL26_04430"
     CDS             complement(2745..2927)
                     /locus_tag="PKL26_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNS82976.1"
                     /translation="MATDPVCYMVVDEDESEYRTSYEGQEYFFCSDFCRKKFEENPGR
                     YIKLARSLDIGPDISC"
BASE COUNT          683 a          783 c          794 g          712 t
ORIGIN      
        1 cggactggat ctcgtcagcc gtctgtcccc ttgccccctc ataggtgatt gcaagggcgg
       61 ttgagagtga gaagggtgag aagaatatat tgccgtctcc gtattccgga ttatctgcca
      121 gtgtattgta gagatcgaat gcaaaccggt tgtttgcatc gaccactgca tcaaggggcg
      181 gggacgaccg aataatcgtg gattgcgggg catgtgtctc cccgaccacg ggaggacccg
      241 ggtgttccgg tgaggtacat cccgcaacga ggagcgaaat gacaaggagt actcccagca
      301 ttgggatggc ggttctgtgc atgtcgatgt gtttggagat acaatctgtt aagcctggcg
      361 ttaggtgcga aaattttctt cgtcataatt tccatcccca aaaaattggc ggggatcagc
      421 cagagggatc gctcaccttc cccatgaaca ggatgttccc cgtctcgttc tcctggatga
      481 gaaagatgaa gggatgatcg gcccggaaca acggcactgt ctcttccaac ggcattgcag
      541 aaagcctcat gaccaccgcc gtggcagcgg cagcctccgt cccttcctcg ttcacctcca
      601 cgaatgcctg gtggatgaca tcgtcgatgt acaggtcctt cgtgccgtcc atcccggaga
      661 agtcggccgc ccgggtgaac gcggtcggca tccccattgc agagagcata ccgggcagcg
      721 agtacttggt ctccatcaca aacttcggga aatatacgat gactctccgg gtcttaagca
      781 aatcatgcag taaagagagc gtgctggtat tcaaggattc ttcaaccatg gagagatcat
      841 cagtcttcgg gagaagcacc agcatcgaga gatctttccc gtcgccggat tcgtatggca
      901 tcgagagcgc ctggagaatg tcggtctctg catagccata gattgcatct tcgtcggttc
      961 gctccatcat ccggactcgc acagtctttc cggttccagt ccggaaatct gcgtccgtgg
     1021 tcttattctc atcaaactgc tttacccatg tccccttgaa ataaatggca ttggtgatca
     1081 caagccgggt taaaggatcg attgtaccgg gctgcagcag atctttgatc ctgtcttctg
     1141 tcaggtcttc aacccacgta ttaatggtca ccctggactc ctccggctta ttgatgaaat
     1201 cgagattggt ggtctttgct ccatagtgct gctcagcagc actgatatac ccggcaagga
     1261 acggatatgt ctgttcagcc cagagagcgt tcgccgttcg gagcatgtat gcattcgcct
     1321 tgctgtttat gcccgaaata atcccggcga acccggatct catgacagtc tggttctccg
     1381 ggaaatgaaa taccgagcgt atctcttccg ctgtcgtccc cctggctccc tcgtacgtga
     1441 tggcaagcgc tgacgatatg gagaatggtg agaagaagat gttgccgtca ccggcttttg
     1501 gatcatctgc gatccttcca tacaggtcaa gggcaaaccg gttgttcgca tcgacgacac
     1561 ttgttgctga accaggaagt gcgggatttg ggggggaggg ggatgtcgtc actgttacgt
     1621 tgggagtggg agtcacctct atggacggcc cgggttctcc cggccccgtg catccggcga
     1681 gaatcactgc aaggagcgcg acagctgcca gcgcaggaat ggctgcaagc gatggattca
     1741 tgcacgtgtc tttttattca gataggttaa atctggcgtt gagtgcggga aatttcggag
     1801 ttatcaatgc agaagatgcc tgcacgtcca aacgttcttg tcacctgctg caaaacaccc
     1861 tgagtgaaat attccgtatt tgagaacatg gaacgatata cgtggaaccc cgctgattat
     1921 gcggagcatt cccatgccca gtacctgtgg gcaggggagg tcatcgcaaa actcgatctg
     1981 aaaggaacag aatcagtcct tgatatcggg tgcggggacg ggaaagtcac cgccctgctg
     2041 gcctcccacc tcccgtcggg tgaggtgatc gggattgaca gttccgccgg catggtgaac
     2101 tgcgccaggg agtctttccc tggatcagat tactccaaca tttcatttct ctgcatggat
     2161 gcgatggata tccggttttc ggaacgtttc gacctcgttt tttcaaatgc caccctccac
     2221 tgggtgcagg accatgcgac gatgctccag ggagtgtatc ggtgcctgaa gaaccgtggc
     2281 aggatcttct tccagatggg tggcagggga aatgcaaggg agatcctcga tttggccggt
     2341 tcactgatcc attccggcag gtggcggaga tatttctcgg atttcagaag cccctataca
     2401 ttcctctctg cagaagaata ccaggtattg ctgagggatg ccggctttct ccctatccgc
     2461 gttgaactta tcccaaaaga catgacacag gtgggatgcg acggcctttg cggctggata
     2521 cgaacgacgt ggctccccta cacggaacga atccccgaac aacaaaaaaa tcgtttcatt
     2581 tccgatcttg cagaatcgta tcttgaaaaa tatcctcctg gttcagaagg cctgattcac
     2641 gtcgggatgg tccgcctgga agtggaagcc caaaaacccg gataataatg cagatacccc
     2701 ccggccccga cagtccactc ccccaaaaag tgaaacggaa acattcaaca actgatgtcc
     2761 ggcccgatat ccaatgatcg tgccagtttt atatacctcc cggggttctc ctcaaatttc
     2821 ttcctgcaga aatccgagca gaagaaatat tcctgccctt cgtaggaagt ccggtattcc
     2881 gattcgtcct catcgacaac catgtagcag acaggatctg tagccatgta tcatgcacac
     2941 cccctgtaat tcgtgcatta tacatcagga ga
//
LOCUS       DAOQVJ010000164         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39016_length_2965_cov_4.611684, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000164 DAOQVJ010000000
VERSION     DAOQVJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39016_length_2965_cov_4.611684"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKL26_04435"
     CDS             <1..312
                     /locus_tag="PKL26_04435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNS82977.1"
                     /translation="ISDLIPGAVFSEREKQEFLGVKVSGIPDGRRLFLPEDFPEGIYP
                     WRRDGTGIPDGMVKDLWASKRPTDRPAPPLPEAQACEMETEEAPPSPAGREHGKEGGQ
                     S"
     gene            309..1538
                     /locus_tag="PKL26_04440"
     CDS             309..1538
                     /locus_tag="PKL26_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNS82978.1"
                     /translation="MSDESKKAPYIVPIGPNHPALKEPVMFTFEMEGEMVRDVDFAPG
                     QTHRGIEWMGMRRNPVQIVHLTDRICGICGVSHTFAFARAVEQIAGIDVPERADYIRT
                     IIAEFERIQSHLLWAGVAAHELGFDSLLHLTWRVREQSMDVIENLTGNRVNYGIVQVG
                     GVRRDIVPEQHQVIEDCLTTYEGLLGKLLNLFLDDKTIRMRCRDCGVLSKKEALELCT
                     VGPTARASGVNVDLRQDYPYAAYGDLDVKAILPDQHMGETHGDVYDRIVVRLLEVGQS
                     CQIIRQCLSEMPSGPVTAEEKLAKLLAVCKKATGEAIGRVEAPRGECFHYVRMEGKEA
                     PHTWKVKASSYSNLMSWIPMLRGEQIADIPIIVASIDPCLSCTDRVAVVRGGQQSILT
                     KEDLHRLSVDKTRRLQR"
     gene            1535..2554
                     /locus_tag="PKL26_04445"
     CDS             1535..2554
                     /locus_tag="PKL26_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNS82979.1"
                     /translation="MSPVDLAVMLSWFVLLSLFALAAGLFLLGVDRKLAAHMQARIGP
                     PIRQPFIDVAKLLQKENVVPANAISAVFHLSPLIALASSIVILFYLPVGSLLPVLGMY
                     GDLVLVMYLLTVPALAMVAGGFASGSPYATVGAQREMVTMIAYEFPLAVVVIGVAWRL
                     GAAGLSSPFALGTMAANPIWTVVGPLGIAGILLLLLVLAVVTPAELSRIPFDTQEAET
                     ELAGGLLVEYSGRNLAMFYLAQGVKTLVLAAVIVAIFFPWNLSGFLAMPPWLALPADI
                     LFFLVKAILVLFFSVTLVRVGMARLRINQVVSVYWIYLSVIGLTGLLLLMADAALPTL
                     MGVGA"
     gene            2551..2955
                     /locus_tag="PKL26_04450"
     CDS             2551..2955
                     /locus_tag="PKL26_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS82980.1"
                     /translation="MKFLPLFPELVRQLFREPATNRFPAKYLPPTVTGFLKAVGEGKA
                     TIVPPVPTPPNFRGKITYKNDCCIGCGLCVKVCPSHAIELIPETKQIRVWVGQCIFCS
                     QCTSICPKGGLSMGDEFLLADEDRYSENLIVE"
BASE COUNT          563 a          918 c          898 g          586 t
ORIGIN      
        1 atctccgacc tgattcccgg agcagtcttt tccgaacggg agaagcagga gttcctcggt
       61 gtgaaggtct cgggcatccc cgatggccgg cggctcttcc tccccgagga cttcccggaa
      121 ggcatctacc cctggaggag agacggcaca ggaataccgg atggcatggt aaaggacctc
      181 tgggcctcga agaggcctac agacaggccg gcaccacccc ttccagaggc acaggcatgt
      241 gaaatggaga ccgaagaggc ccctccctcg cccgcaggcc gggagcacgg aaaggagggc
      301 ggtcaatcat gagcgacgag tcgaagaaag caccctacat cgtccccatc gggccaaacc
      361 accctgcatt gaaggagccg gtgatgttca ccttcgagat ggagggggag atggtcaggg
      421 acgtggactt tgcccccggg cagacgcacc ggggtattga gtggatgggg atgcgccgga
      481 atcccgtcca gatcgtccac ctcaccgacc ggatatgcgg catctgcggg gtctcccaca
      541 cgttcgcatt cgcccgcgca gtcgagcaga ttgccgggat cgacgtcccc gagcgggcgg
      601 attatatccg caccatcatt gccgagttcg agcgtatcca gtcgcacctc ctgtgggcgg
      661 gcgttgccgc ccacgagctc ggctttgact cgcttcttca cctcacctgg agggtgcggg
      721 agcagtcgat ggacgtcatt gagaacctga ctggaaaccg ggtgaactac ggcatcgtgc
      781 aggtcggggg cgtgcgccgg gacatcgtcc cggagcagca ccaggtcatt gaggactgcc
      841 tcaccacgta cgaggggctg ctcggaaaac tgctgaacct cttccttgac gacaaaacaa
      901 tcaggatgag gtgccgggac tgcggggtgc tctcaaagaa ggaggcgctc gaactctgca
      961 ctgtggggcc gactgcgcga gcctctggcg tcaacgtaga tcttcgccag gattatccat
     1021 atgctgcata cggcgacctc gatgtgaagg ccatactccc tgaccagcac atgggcgaga
     1081 ctcacggcga cgtctacgac cggatagtgg taaggctgct cgaagttggc cagtcctgcc
     1141 agataatccg gcagtgcctc tcagagatgc cttcagggcc ggtcactgcc gaggagaaac
     1201 tcgcaaaact ccttgccgtc tgcaagaagg caaccggcga agcaatcggg agggtcgagg
     1261 ccccgcgcgg ggagtgcttc cactacgtca gaatggaagg aaaggaagcc ccgcacacct
     1321 ggaaggtgaa ggcatcctcg tacagcaacc tgatgtcctg gatcccgatg ctgcggggcg
     1381 agcagatcgc cgatatcccc atcatcgttg cctcaatcga tccctgcctc tcctgtaccg
     1441 accgggttgc ggtggtcagg ggcgggcagc agagcatcct cacgaaggag gatctccatc
     1501 gcctgtccgt ggataagacc aggaggctgc agcgatgagt cctgtcgacc ttgcggtcat
     1561 gctaagctgg tttgtgctgc tctccctttt tgcgctggcg gccggcctct tccttcttgg
     1621 cgtggaccgg aaacttgcgg cccacatgca ggccaggatc ggcccgccga tccgacagcc
     1681 gtttatcgac gtcgccaagc ttctgcaaaa agagaacgtg gtccctgcaa acgccatttc
     1741 ggccgtcttc cacttatcac cgttgatcgc gctggcatca agcatcgtca tcctcttcta
     1801 cctgccggtg ggaagcctcc tgccggtgct cggcatgtac ggagacctgg tgctggtgat
     1861 gtatctcctg accgtgcccg cacttgcgat ggtcgcaggg ggttttgcgt caggatctcc
     1921 atatgcaacg gtcggagccc agcgcgagat ggtgaccatg atcgcctacg agttcccgct
     1981 cgcggttgtc gtgatcggtg tcgcctggcg ccttggggcg gccggtctct cctcgccgtt
     2041 tgcgctcggg accatggcag caaaccccat atggaccgtg gtcgggccac tgggaatcgc
     2101 aggaatcctc cttctcctcc tggtgctcgc ggtcgtcacc ccggccgagc tctcccggat
     2161 cccattcgac acccaggaag ccgagacgga acttgcagga ggcctgctgg tggagtactc
     2221 gggcaggaac cttgccatgt tctaccttgc ccagggggta aagacactcg tgctcgcggc
     2281 cgtgatcgtt gcaatctttt tcccgtggaa cctctcggga ttcctcgcga tgcccccgtg
     2341 gctggctctg cccgcggata tcctgttctt cctcgtaaaa gcaatcctgg tgctgttctt
     2401 ctcggtcacc ctcgtccggg tgggaatggc aaggttacgc atcaaccagg tagtctcggt
     2461 ctattggatc tacctttcag tcatcgggct tacaggcctc cttctcctga tggcagatgc
     2521 cgcgcttccc acgctcatgg gggtgggggc atgaaattcc tgcccctgtt ccccgaactc
     2581 gtccgccagc tcttccgcga gcctgcgacc aaccgcttcc cggccaagta tctcccgcca
     2641 acggtgacag gtttcctgaa ggctgtggga gagggaaagg ccacaatcgt tccgccggtc
     2701 ccgacccccc cgaacttccg gggcaagatc acctacaaaa acgattgctg catcgggtgc
     2761 ggactgtgcg tgaaggtctg cccctcccac gcaatagagc tcatccccga aacaaaacag
     2821 atacgggtct gggtcgggca atgcatcttc tgctcccagt gcaccagtat ctgcccgaag
     2881 ggcggcctct ccatgggtga tgagttcctc cttgccgacg aggatcggta ctcggagaac
     2941 ctgatcgtgg agtgaggaag ggttc
//
LOCUS       DAOQVJ010000165         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39035_length_2964_cov_4.112754, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000165 DAOQVJ010000000
VERSION     DAOQVJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39035_length_2964_cov_4.112754"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="PKL26_04455"
     CDS             complement(<1..2182)
                     /locus_tag="PKL26_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS82981.1"
                     /translation="MRDTRWRLPFSYLLMGILFLVIVAVLIAGLWINYENSKNHTVEN
                     AERLRVMTESHMDNSFRMIDTGLKLYDNTYNQKMQDAFGIVMAEYLQAGGEPSLMDLP
                     SLKNRIGGMEIHVINDQCVIEHSSSPGDIGLDFSIIYPDFCVYLHKIQNTSGYYPDRV
                     VMEWFTGNLTKYGYMPTPDHQYILELGLHSDAFTRDRMELQYRDVIDEVRTFNPYLEE
                     VLLFQKQKRLIYNTTYIPTLEESEMLDYILWKNRSTQIVKDNGAGKTIVWQVIDLRDP
                     DYAADKSVFAKLTYNDALVREELDQIAILHGFAALLVILSGGLIAIMVSRKLSRPIEK
                     LVEDVNIIAEGDLNHAVRPAPGFELSNLSGSIQVMVDRLKAEIRQCEASEERFMELVQ
                     LLPQAIFETDLNGNITFANPIAFEVFGYNPEDFENGINIFDILAPDEQILARERFGEI
                     LAGKRSQGSEYTGIRKDGSTFPIMVYTAVRKEEGAVTGTRGTIVDITRLKKVEEEIRK
                     LNTELEARVAQRTSELEYATHEMEAFTYSVSHDLRGPLRAIDGFSHILRESASTGLDE
                     REQHCIDVIRKNVELMDSLIDGLLALSRMGRQELKREWVSPETLVREVVAEVLNQAPG
                     RKIDVMIDTLPECYADPVMLWQVFANLIGNAVKFTRKTENARIEIGAFFRNSMAVYYV
                     RDNGIGFDMKYVNTLFKPFQRLHKTEEYEGSGVGLAIVNRIIRRH"
     gene            2581..2964
                     /locus_tag="PKL26_04460"
     CDS             2581..2964
                     /locus_tag="PKL26_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82982.1"
                     /translation="MPTTAQPTASPTVATPVATPTLGYIEGPLPAQYMVDVQVDRNTV
                     AITPSIIATYRGGKGTNFVYSVDVTVTRSDGQVITKSLERPQVNQQVEIEGTRGTDRV
                     EVFVNLVNGERYRIYDQSLAFRTYN"
BASE COUNT          661 a          808 c          740 g          755 t
ORIGIN      
        1 catgccgcct gatgatgcgg ttcacgatag cgaggccgac accggagccc tcgtactcct
       61 ccgttttgtg cagacgttgg aatggtttga agagggtatt tacatacttc atgtcaaacc
      121 cgataccgtt gtcccggaca tagtacactg ccatggaatt cctgaagaaa gctcctatct
      181 ctatccgtgc attctccgtt ttcctggtaa attttaccgc atttccaatg agattggcaa
      241 acacctgcca cagcatcacg gggtcggcgt agcactccgg taatgtatcg atcataacat
      301 cgatcttccg gcccggggcc tggttcagta cttctgccac gacttcccgg accagcgttt
      361 cgggcgatac ccattcccgc ttcagttcct gcctgcccat acgcgagagt gccaaaagac
      421 cgtcgatgag gctgtccatc agttccacgt tcttccttat cacgtcgatg caatgctgtt
      481 ccctctcatc aaggcctgtg ctggctgact cccgtagtat atgggagaac ccgtcaatag
      541 cccgaagcgg tccgcgcagg tcatgcgaga ccgaataggt gaacgcctcc atttcatggg
      601 tcgcatattc gagttctgat gtccgctgcg caacccgggc ctcaagttct gtgttcaact
      661 ttcttatctc ttcttcgacc ttcttcaagc gggtgatatc cacaatcgtt ccccgcgttc
      721 cggtgacggc tccctcctct ttccgtaccg cggtgtatac catgatgggg aatgtgctgc
      781 catctttccg tatccctgta tattcagaac cctgggacct cttccctgcc aggatctccc
      841 cgaatctctc tctcgccagt atctgttcgt cgggtgcgag gatgtcgaat atgttgattc
      901 cattctcgaa atcttccggg ttatacccaa agacctcgaa cgcgatgggg ttggcaaaag
      961 tgatgttccc gttgagatcg gtctcaaaga tcgcctgcgg aaggagctgg acgagttcca
     1021 tgaagcgctc ctcgctcgcc tcgcactgcc ggatctctgc cttcagccgg tcgaccatga
     1081 cctggatgct cccggaaagg ttggaaagtt cgaagcctgg agcgggcctg accgcatgat
     1141 tcagatcccc ttctgcaatt atgttgacat cctcgactag tttctcgata ggcctcgaga
     1201 gttttcgcga gaccatgatg gcgataagcc ctcctgaaag aatgacgagc aatgcggcaa
     1261 agccatgcag gattgcaatc tggtccagtt cttctctcac cagtgcatca ttataggtga
     1321 gttttgcaaa tacactcttg tcagcggcat aatccgggtc acggaggtcg atgacctgcc
     1381 agacgatggt ctttcccgca ccattgtcct tcactatctg ggtagaccgg ttcttccaga
     1441 ggatgtaatc gagcatctcc gattcttcaa gggtgggaat ataggtggta ttgtagatca
     1501 gcctcttctg tttctggaac aggaggactt cttccaggta aggattgaat gtcctgacct
     1561 cgtctatcac atcgcgatat tggagttcca tccgatccct cgtaaatgcg tctgaatgaa
     1621 gcccaagctc gagaatatac tggtgatcgg gtgtgggcat atatccgtat tttgtgaggt
     1681 tcccggtgaa ccattccatc acgaccctgt cagggtaata gcccgaagtg ttctgaatct
     1741 tgtggagata tacacagaaa tcggggtaga taattgaaaa atcgagccct atatcccccg
     1801 gtgacgatga atgctcgatg acacactgat cgttgatcac gtggatttcc atccccccga
     1861 tcctgttttt cagggaggga aggtccatca gggagggctc tcctcctgcc tggagatatt
     1921 ctgccatgac gattccgaac gcatcctgca tcttctggtt gtaggtgttg tcgtacagct
     1981 tcaacccggt gtcaatcatc ctgaacgaat tatccatgtg cgattccgtc atgacccgga
     2041 gccgttctgc attctcgacg gtatggttct tcgaattctc ataatttatc cataacccgg
     2101 cgattaatac agcgacgatg acaagaaaaa ggattcccat gaggaggtat gaaaaaggca
     2161 gtctccaccg ggtatctcgc atcaaatccc cctttcaatg ggatatatgc aattatgtta
     2221 tttaacagga atcgtcagcc agccgggtca tgtttaggat ggatattcat cccaaagact
     2281 tcagtattcg aacgtaaccc ctctcatcat atgcgttgga aggtgcaatc cggtgaatcc
     2341 ggacccggaa ggtcctgtgt gtagccagca taaagttccc ctcttttgat ttcggcagtc
     2401 ctgtaaggtg accttgcaaa atgaaaaatg gatcctcaga agactcttat aattgcggga
     2461 tgaagctcta ctcatgagag cgatcttctt tgtggtgctc tgtgcgcttg caggactcct
     2521 tgtccttggc gccggctgca cccgccatca acggggccgg ccacgcaggt ccagacacct
     2581 gtgcccacca cggcacagcc caccgcaagc ccgactgttg cgacccctgt cgcgaccccg
     2641 acactcggat acatcgaggg tccgctgcct gcacagtata tggtcgatgt ccaggtcgac
     2701 cgtaatactg ttgccatcac tcccagcatc atcgcgacat accggggtgg aaaaggcacc
     2761 aattttgtat attctgtaga tgtcacggtt accaggtccg atggccaggt gatcacgaag
     2821 agcctggaga ggccgcaggt gaaccagcag gtggagatcg aagggacacg gggcaccgac
     2881 cgggtagagg tgttcgtcaa ccttgtcaat ggggagcgat acaggattta cgaccagtcc
     2941 ctggcattcc ggacatacaa ttaa
//
LOCUS       DAOQVJ010000166         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39037_length_2964_cov_4.045376, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000166 DAOQVJ010000000
VERSION     DAOQVJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39037_length_2964_cov_4.045376"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..300
                     /locus_tag="PKL26_04465"
                     /pseudo
     CDS             196..300
                     /locus_tag="PKL26_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            316..648
                     /locus_tag="PKL26_04470"
     CDS             316..648
                     /locus_tag="PKL26_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNS82983.1"
                     /translation="MTEVTHAMGAVGNAAIEVFQAQGTYPTATNLAMVQTSMGITVPQ
                     TYVSDATVTPNNATAPFYADIQVTFNGEIQQDWSGRVLTLRVQQGARSDWNPENSDTL
                     PAEFIPRE"
     gene            921..1613
                     /locus_tag="PKL26_04475"
     CDS             921..1613
                     /locus_tag="PKL26_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNS82984.1"
                     /translation="MLQQTQVERVLGKYPSFIEAFPDFHSLADASLKVVLSLWQGLGY
                     NRRAVNLWKTAQLVVEEHYGNLPDSPDELIQLPGIGRATAGAIRAFAFNRPVVFIETN
                     IRRAFIHHFFQDRMDVRDREILPLVEETLDHENPRQWYYALMDYGAMLGRLPSNPNRR
                     SAHYARQTPFEGSDRQVRGRVIGALLEQGRLTGKGLSSQLNIEGDRLRTILAGLEKDG
                     LVVRAGTGYRLP"
     gene            complement(1628..2209)
                     /locus_tag="PKL26_04480"
     CDS             complement(1628..2209)
                     /locus_tag="PKL26_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82985.1"
                     /translation="MGRKERRIILICQCLVNPYCRVHILGQNFPLSLELTDYLLRKNV
                     GIVQYPCPETTAMGLMRNPQGRQQYDNIFFRNHCKELLQVPMLMVREFLKNRYRLCCF
                     VGLENSPTCGIHWGRHKVNRYGTESPNPDGQYGKDPQDPVLRGIMAEILEEELAREGI
                     TTPFLELPALSQADSEKRKRFWQDLEDAVSPVP"
     gene            2369..>2964
                     /locus_tag="PKL26_04485"
     CDS             2369..>2964
                     /locus_tag="PKL26_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNS82986.1"
                     /translation="MEDTLQGLFFPESIALYGIPRGLKPGKVFLLGLLDQGFGGPMYL
                     IHPSATEIDGFRAYPGLDHVPGAVDLVIVMSPRETVFAVLEECARKHVKAVILYTSGF
                     SELGDEAGREAESRMRRIARDGGFRILGPNCMGVLSPGSRIAPFPGLPKTPGPLGFIS
                     QSGSLLNLFVNLCTRKGLAFSHAVSSGNGADIDMADLLA"
BASE COUNT          638 a          828 c          860 g          638 t
ORIGIN      
        1 cggaatgaat gcagtttctt gaaaacacgg atgataatat ttctctcaag tattttatag
       61 gggtgccgta tatatataat gaaatgaaaa ttcgtatgat taatgcaatg attcatacgg
      121 gccgcacggt acaaaggctt aatcatatct taagcgattc cactaaccaa aaggaggaat
      181 gaggctatgt taaaaatgtt cagatcaagt aaaaaaggtt ttacgctcat cgagttgatg
      241 atcgtcgtgg cgatcatcgg tatcctggca gccatcgcaa tcccggcata tagcaactac
      301 acgagaaagg cgagactgac agaagtcacc cacgccatgg gtgcagtggg caacgcggcc
      361 attgaggtat tccaggcaca ggggacctac cccacagcaa caaaccttgc catggtacag
      421 acctccatgg gaattaccgt tcctcagacg tacgtgtctg acgccacagt tacgccgaac
      481 aatgcgacag ctcctttcta tgctgacatt caggtcacct tcaacggcga gatccagcag
      541 gattggagtg gtcgcgtcct taccctgagg gtccagcagg gtgcacggtc ggattggaac
      601 ccggaaaaca gcgacaccct tcctgctgag ttcatcccaa gagagtagtt ttatcattgg
      661 aataaggacc tacaggggta tggcacgacg tgccataccc cttgttcttt ttctgaagca
      721 ccaccgaccc aatcagattg gtaatgccga tagtcatagc agataggtag actccatgat
      781 acgataccag tattgtgcag atgcatgctt cagtattatt agagtttcaa ctgttgatcc
      841 atagatatca ccgggaccac gccaggacca tgccatggag gaaaacagag gatccttaca
      901 gagtcctcgt ctcggaattc atgctccagc agactcaggt cgagcgcgtc ctcggcaagt
      961 acccctcgtt catcgaggca ttccctgatt ttcattcact tgccgatgct tctctgaagg
     1021 ttgtcctgtc gctctggcag gggctcgggt acaaccgcag ggccgtgaat ttgtggaaga
     1081 ccgcgcagct ggttgttgaa gaacattatg ggaatcttcc ggattcacca gacgagctga
     1141 ttcaattgcc cggcatcggc cgggcgacgg caggggccat ccgtgccttt gccttcaaca
     1201 ggccagtggt gttcatcgaa accaacatcc gcagggcctt catccaccac tttttccagg
     1261 acaggatgga tgtccgtgac agggagatcc ttcccctggt ggaagaaacc ctggaccacg
     1321 aaaatccccg gcagtggtac tatgcactca tggattacgg cgccatgctg gggaggcttc
     1381 catcgaatcc caaccggaga agtgcccatt atgcaaggca gacccctttc gagggctccg
     1441 accggcaggt gcggggcagg gtcatcgggg cgctcttgga acagggcaga ctcacaggga
     1501 aaggcctttc ctcgcagctg aacatagagg gtgacaggct ccggacgatc ctcgcggggc
     1561 ttgaaaagga cggcctcgtc gtccgggcgg gaaccggcta tcggctcccc tgatatgccc
     1621 gcaccggtca gggcaccggc gagaccgcat cttccaggtc ctgccagaac ctctttctct
     1681 tttcggaatc tgcctgtgag agggcgggga gctccagaaa gggtgttgtt attccctccc
     1741 tggccagttc ctcctccagg atctcggcca tgatcccccg gagcaccgga tcttgcgggt
     1801 ccttcccata ctgcccgtcc gggttggggg actcggtccc gtatcggttc accttgtgcc
     1861 tgccccagtg gatcccgcag gtggggctgt tctccagccc gacgaagcag cacaggcggt
     1921 agcggttctt gaggaactcc ctgaccatga gcatgggcac ctggagcagt tccttgcagt
     1981 ggttgcggaa aaagatgttg tcgtactgct ggcggccctg ggggttgcgc atgagcccca
     2041 tggccgtggt ctccgggcag ggatactgga cgatgcccac gttctttctc agcaggtagt
     2101 cggtcagctc cagggagagg gggaagttct gccccaggat gtggaccctg cagtaggggt
     2161 tgaccaggca ctggcagatc aggatgatcc tgcgctcttt cctgcccatg gaggtgctcc
     2221 tttcactgat caatataagc gcatgcgggg ttaatgcaat gtgaacaaac ctcgtactat
     2281 tcttttaccg tgcgttgaag aaggaggaaa gaggtggtac catgccggtc agagtgcatt
     2341 gacttgtcat gggcaaggag gttgtgccat ggaggatacg ctccagggat tatttttccc
     2401 cgaatccatt gcgctgtacg gcatccccag ggggctcaag cccggcaagg tctttctgct
     2461 ggggctcctg gaccagggct tcggcggacc catgtatctc atccaccctt ccgccacgga
     2521 gatcgacggg ttcagggcgt acccgggcct tgaccacgtc cccggagccg tggacctcgt
     2581 catcgtcatg tctcccaggg agacggtctt tgccgtcctg gaggaatgcg cgaggaagca
     2641 cgtcaaggcg gtcatcctct acacctccgg tttttccgag ctgggggacg aggcagggcg
     2701 cgaggccgaa tcgcgcatga ggcggatcgc ccgcgacggg ggcttccgca tcctggggcc
     2761 caactgcatg ggcgtgctgt cgccgggctc ccggatagcc ccgtttccgg gcctgccgaa
     2821 gacaccggga cccctcggat tcataagcca gagcgggtcg ctgctgaacc tcttcgtcaa
     2881 cctgtgcacg cggaaggggc tggccttcag ccatgcggtc agcagcggaa acggggccga
     2941 catagacatg gccgacctgc tcgc
//
LOCUS       DAOQVJ010000167         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39059_length_2963_cov_4.025103, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000167 DAOQVJ010000000
VERSION     DAOQVJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39059_length_2963_cov_4.025103"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1736
                     /locus_tag="PKL26_04490"
     CDS             57..1736
                     /locus_tag="PKL26_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNS82987.1"
                     /translation="MLFGVLFTIMEELSVLIGGKAGDGINSAGMVVAHLFNRMGYRVY
                     MYFDYPSLIKGGHNFAIVRAAKEKIGAHRSQVDFILALNQDAVDFHRDMVHRKTVVLS
                     DRSKVRDEGIGIDLPQILKEEKAPPVMGNSCIIGAFAKAAGIEWEVLEEVFARQIPKA
                     LPTNLRVARRGYDAASECCRIEPLGSSPLPVISGNEAIGLGLIHGGLDAYVAYPMTPT
                     SNILHFMAEVAGAHDLLVFHPENEIAVMLMALGLGFAGKRTAAGTSGGGFCLMTEGFS
                     LSGMSEIPVVVVLGQRAGPSTGMPTYTAQSDLQFALHAGHGEFPRLVVAPGDAEQAYA
                     WSDIALRLSWKYQVPSIILCDKTLCEGFYSFTSPDDTMARVDPSPAGSPGPGYLRYGL
                     TESGVSPMLFPPEKDTVIKVNGYTHDQNGITTEKAGLASRMSEKRGRKGSALAEEVLR
                     FKPVESGGVRQSETTLLCWGSTIGVCREVAAGLGLRLVQPVVLSPLPLQALHDALRGT
                     KRLIAVEENAEGQIAMLLARHGIRLDSHIRRYDGRPFAREELEARVREALA"
     gene            1733..2584
                     /locus_tag="PKL26_04495"
     CDS             1733..2584
                     /locus_tag="PKL26_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS82988.1"
                     /translation="MTARNLITDAQNTWCPGCGNFVIQFALKNTIAALEKEGVDTDRV
                     VLVTGIGCHAKMADYLNVNSFYSIHGRTLPVATAIKMANPELVVIACAGDGDCYAEGL
                     DHLIFAAKRNTDITLLVHNNRVYGLTTGQYTPTSPLGFRGRSTPSGTVEEPFNPLEIM
                     LASGATFVARGCTRKMALLQRTIMEGIRHPGFSFIDVLQVCASYFNLSDYYDRSVYEI
                     RDHDERDFEAACQKAREWDYNTDAPIGLGTIYRAKRPTLEERLSAIRGTETDRDATIR
                     NVLEKRT"
     gene            2697..>2963
                     /locus_tag="PKL26_04500"
     CDS             2697..>2963
                     /locus_tag="PKL26_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNS82989.1"
                     /translation="MTDQNLFEMVKQHLCSCSTDLVMDANVEAVLKMPRREMHASIPV
                     RMDDGKIRAFQGFRVQYNDALGPTKGGIRFHPDETIDTIRGLAAL"
BASE COUNT          632 a          837 c          919 g          575 t
ORIGIN      
        1 tgaaagggaa tcgtgtggtt ttttgggaag aacctatatc tttccctgaa aagagagtgt
       61 tgttcggggt gctcttcacg atcatggagg aattatcagt tctcatcggg ggaaaggccg
      121 gtgacgggat caacagtgcc ggtatggtgg ttgcccatct cttcaaccgg atgggatacc
      181 gtgtgtacat gtattttgac tacccgtccc tcatcaaggg ggggcataat tttgcgattg
      241 ttcgggctgc gaaggagaag atcggggcgc accggtcaca ggttgacttt atcctcgcac
      301 tgaaccagga tgccgtggac tttcacaggg atatggttca caggaagacc gtggtgctct
      361 ctgaccgctc gaaggtcagg gatgagggga taggaattga tctcccacaa atcctgaaag
      421 aggagaaggc cccgccggtc atggggaact cgtgcatcat cggggcattt gcgaaggccg
      481 cagggatcga atgggaggtg ctcgaggagg tattcgcacg ccagatccca aaggcgcttc
      541 ccacaaacct cagggtggcg cgccgggggt atgacgcggc cagcgagtgc tgcaggattg
      601 agccgctcgg ctcgtctcca ctgccggtga tctcggggaa cgaggcgata ggccttggcc
      661 tcatccacgg tggcctcgat gcctacgtcg cgtatccgat gacccccaca tccaatatcc
      721 tccacttcat ggccgaggtt gccggggcgc acgatctcct ggtcttccac cccgagaacg
      781 agattgcggt gatgctgatg gcgctcggcc tcggatttgc cgggaagagg accgcggcag
      841 ggacttccgg aggaggcttc tgcctgatga ccgaggggtt ctcactctca ggcatgagcg
      901 agattccggt cgtggtggtg ctcgggcagc gggcgggccc aagcaccggg atgcccacct
      961 atacggcgca gtccgacctg caatttgcat tgcacgccgg tcatggggaa tttccccgcc
     1021 ttgtggtggc ccccggggat gcagaacagg cttatgcctg gtctgacatc gcgctccgcc
     1081 tctcctggaa ataccaggtc ccgtcaatta tcctctgcga caagacgctc tgcgaggggt
     1141 tctacagctt caccagtcct gacgacacaa tggccagggt tgacccaagc cctgccggga
     1201 gcccggggcc cgggtatctc aggtacgggc tgaccgagag cggcgtctcg ccaatgcttt
     1261 ttccccccga aaaggatacg gtgataaaag tcaacggcta cacccacgac cagaatggga
     1321 tcaccactga gaaggcgggt ctcgcctccc ggatgagtga aaagaggggg cgaaagggat
     1381 cggcgctggc agaggaggtt ctccggttca aaccggtaga atcaggcggg gtcaggcaat
     1441 cggaaaccac gctcctctgc tgggggtcca cgatcggagt ctgccgcgag gttgcggctg
     1501 gcctggggct ccggctggtg cagccggtcg tgctctcgcc cctccctctg caggccctgc
     1561 atgatgcgct gcgcgggacg aaaaggctga ttgcggtaga agagaacgcg gaggggcaga
     1621 tcgcaatgct gctcgcacgg cacgggatca ggctggactc tcatatcaga cggtacgacg
     1681 gaaggccgtt tgcgcgcgag gaactggaag cacgcgtcag ggaggcgctc gcatgactgc
     1741 gaggaacctt atcaccgatg cccagaacac ctggtgcccc gggtgcggca attttgtcat
     1801 ccagttcgcc ctgaaaaata ccattgcggc ccttgagaaa gagggagttg atactgacag
     1861 ggtcgtgctc gtaaccggga tagggtgcca tgcaaagatg gcggactacc tcaacgtgaa
     1921 cagcttctac tcaatccacg ggcgcaccct gccggttgca accgcgatca agatggccaa
     1981 ccccgagctc gtggtcattg cctgcgcagg tgacggggac tgttatgccg aaggccttga
     2041 ccacctcatc tttgccgcaa aacggaacac cgacatcacc ctccttgtcc acaacaaccg
     2101 ggtctacggg ctgaccacgg ggcaatacac cccgacttcc ccgctcgggt tcaggggccg
     2161 ttcaacgcct tcggggactg ttgaggaacc tttcaatccc ctggagatca tgcttgcatc
     2221 cggcgccacg ttcgttgcga gaggatgcac acggaagatg gctctcctgc agcgcacgat
     2281 catggaaggc atccgtcatc ccggcttttc tttcatcgac gtgctccagg tatgcgcatc
     2341 ctacttcaat ctgtctgact actacgacag gagtgtatac gaaatcaggg accacgatga
     2401 gcgggacttt gaggccgcct gccaaaaagc cagggaatgg gattacaaca cggatgcacc
     2461 gatcggcctt ggaacgatct accgggcgaa gagacccacc ctggaagagc ggctctctgc
     2521 catccggggc accgaaacgg acagggatgc caccatccgg aatgtgctgg agaagagaac
     2581 gtgaagctga atggctctca ttcactcaac caagccgtac ctgctggcat ggtgcggcat
     2641 ttccgtgcct ttaaataccg gatggctctg aatgtcttcg taagaggggg aaacgaatga
     2701 ccgatcagaa cctcttcgag atggtcaagc agcatctctg ctcatgctcg accgaccttg
     2761 tcatggatgc aaacgtggag gcagtgctga agatgccgcg cagggagatg catgcatcaa
     2821 tccccgttag gatggacgac gggaagatcc gggcattcca ggggttccgc gtgcagtaca
     2881 acgatgcact cggtccgaca aagggcggaa tcaggttcca cccggacgag accatcgaca
     2941 ctatccgggg tcttgccgcc ctg
//
LOCUS       DAOQVJ010000168         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39061_length_2963_cov_3.756534, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000168 DAOQVJ010000000
VERSION     DAOQVJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39061_length_2963_cov_3.756534"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKL26_04505"
     CDS             complement(<1..487)
                     /locus_tag="PKL26_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HNS82990.1"
                     /translation="MDALTPEEGALAVRLARSVLEHAIGGKPEAVFDLPPVFREKRGV
                     FVTLTSQGELRGCIGFPYPHLALEKAVREAAYAAAREDPRFSPVGKAELARIRVEVTV
                     LTVPMLLEAEPLDRPRAVQVGRHGLIVRGFGRSGLLLPQVPVEWKWDSREFLDHTCMK
                     AG"
     gene            522..873
                     /gene="rnpB"
                     /locus_tag="PKL26_04510"
     ncRNA           522..873
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKL26_04510"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            937..1497
                     /locus_tag="PKL26_04515"
     CDS             937..1497
                     /locus_tag="PKL26_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS82991.1"
                     /translation="MHIPTPEEIKARRELLGMKQAELAERAGISQSMVARIESGSVDP
                     RASTLAKIVHVLNIMARPRTTAARVMHTPVISVGVEDTITHAVDIMEKYNISQVPVLE
                     NGVPVGCISESAIVAAIEEQGGHRTQGEQVKHYMESGFPTVPHDMDIETVIRILQHHH
                     AVLVMEEGRVRGVITKHDLIPLIMDR"
     gene            complement(1577..2050)
                     /locus_tag="PKL26_04520"
     CDS             complement(1577..2050)
                     /locus_tag="PKL26_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNS82992.1"
                     /translation="MGTFICTRCGKCCISLGRHIRIERSLSSVQHYVRNAISGEVTLV
                     TIHPERRALFSEKPDEGWCPFLRREQDGRFTCTIYENRPAICRNFRCLNMVISSQDGT
                     EVGQIVGKATITTKDPVLKELWNNLKKQMPMSDPRWFEYMQKELKMHGYTLEPVT"
     gene            2169..2384
                     /locus_tag="PKL26_04525"
     CDS             2169..2384
                     /locus_tag="PKL26_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82993.1"
                     /translation="MPVEGVLSSPGMSACRVGTSRAATAHGVSGFAQQQGVRIPPILH
                     DMDIETGLHDTPAPPQGAGDEGGGPVT"
     gene            complement(2423..>2963)
                     /gene="tpiA"
                     /locus_tag="PKL26_04530"
     CDS             complement(2423..>2963)
                     /gene="tpiA"
                     /locus_tag="PKL26_04530"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNS82994.1"
                     /translation="TYMNIHPMSMHYSLPVYAQHVDAAAPGAHTGSVTVEGIRSAGAV
                     GSLVNHSEHRLTLADIDAAVSALRSAGLISVVCSNNVATSAAAASLGPDYVAIEPPEL
                     IGGTVSVSQANPEIITGTVTAVRNVNPSVRILTGAGIHSGDCVKVAVELGTAGVLLAS
                     SVVKAKAPISVLRDLVSKL"
BASE COUNT          630 a          879 c          882 g          572 t
ORIGIN      
        1 gccccgcttt catgcaggtg tggtcaagga actcccggct gtcccatttc cattccactg
       61 ggacctgcgg aaggaggagt ccgctccggc caaatccccg cacgatcagt ccatgcctgc
      121 cgacctggac tgcccgcggg cggtccaggg gttcggcttc cagcagcatg ggaacggtaa
      181 gcactgtcac ctcaaccctt attcgtgcca gttctgcctt cccgacaggg gaaaacctcg
      241 ggtcctccct tgctgcagcg tacgctgcct ccctgacagc cttctccagg gcgaggtgcg
      301 ggtaagggaa accgatacag cccctgagtt ccccctgcga ggtaagagtc acgaagaccc
      361 cccttttctc cctgaataca ggggggaggt cgaaaacggc ctccggtttt ccgccaattg
      421 cgtgctccag gaccgagcgt gccagccgca ccgcgagtgc cccctcttct ggcgtgagcg
      481 catccatcag gtgtaaataa ggtgccgccc tattaaatag ggagagggag agtgcggccg
      541 acggtggcac atggtgctgc tgaggaaagt ccccccacca gctgagcacg cagccgccac
      601 gcaagtgcgg gtggcgagag tcacggcaat ggcacagaaa cgacacggcc tgcccccagc
      661 gatgatgcga tcgagcccca ccaaaaaggg caatgcatcc ccgcttttca tgggcgcgga
      721 tgactcgctg atgcataagg gcaggaatcg atgaaacggt gaatccctgc gggtgcaagc
      781 caaaacaggg catgtaggct gctcagtgcc tgcccgggta tggcgcgtag ctgaatgccg
      841 caccgaacag aagggggctt actcctccca ctctattcct gtatgcctat tctgcagtac
      901 atttatccac tcatatgcac cagggtaata ccactgatgc atatccccac tcccgaagag
      961 ataaaggcaa ggcgggaact cctgggcatg aaacaggctg agctcgcgga gagggcaggg
     1021 atcagccagt cgatggttgc gcggatcgag tccgggagcg tcgaccccag ggcaagcacc
     1081 cttgcaaaga ttgtgcacgt cctcaacatc atggcaagac caaggaccac cgccgcacgc
     1141 gtgatgcaca caccggtcat cagcgtcggg gtggaggaca ctatcaccca tgctgtggat
     1201 atcatggaga agtacaacat ctcccaggtc ccggtgctcg agaacggggt gccggtcggg
     1261 tgcatctctg aatcggcgat cgtggctgcc atcgaggagc agggaggtca ccgtacccag
     1321 ggcgagcagg tgaagcacta catggagtca gggttcccca ccgtccccca cgacatggac
     1381 attgagacag tgatacggat cctccagcac catcacgcgg tgctggtgat ggaagagggg
     1441 cgggtccgcg gcgtcatcac caagcacgat ctcatccccc tgatcatgga ccgctagagc
     1501 ttcgggacga ggtcagggaa catgctgatg ggagccttgg ccgtgactat acctgatgcc
     1561 agagcaccca tgcggttcag gtcacaggtt cgagggtgta gccgtgcatc ttcagttctt
     1621 tctgcatgta ctcaaaccat ctcggatcgc tcatcggcat ctgttttttc agattgttcc
     1681 aaagttcttt cagtacaggg tccttggtag tgatggtggc ttttcccacg atttgtccca
     1741 cttccgttcc atcctggctg gaaatgacca tgttgaggca ccggaaattc cggcagatgg
     1801 caggccggtt ctcgtaaatg gtgcaggtga agcgcccgtc ctgttctctc cgcaggaacg
     1861 ggcaccaccc ctcatctggc ttttctgaaa agagggcacg cctttccgga tggatggtca
     1921 caagggtaac ctctcccgag atcgcattcc ggacgtaatg ctgcactgat gagaggctcc
     1981 gctcgatccg gatatgtcga ccaaggctga tgcagcactt cccgcaccgg gtgcagataa
     2041 atgttcccat gcctacctgg ggttttcctg ggaaatatca taaaggtgca taaaaggaca
     2101 tccccgtgag agaatctttg gccgccctgc tcttctcccc atggtatgag tgaggagtat
     2161 caacattgat gccagtcgaa ggtgttctgt cttcacccgg gatgagcgcc tgccgggtgg
     2221 gaacttcaag ggcggccacc gcacacgggg tgagcggctt tgcgcaacaa cagggagtca
     2281 ggattccccc catcctccac gacatggaca tcgaaacggg attacatgat actcctgcac
     2341 cgccacaagg tgcaggtgat gaggggggcg ggcccgtgac gtaatcacca cgcaatgacc
     2401 tcatccccct gatcagggac cgctagagtt ttgaaacgag atcccggagc acgctgattg
     2461 gggcctttgc cttgaccacg ctcgatgcca ggagaacgcc tgctgtgccc agctcgacgg
     2521 caaccttcac gcagtcacct gagtggatcc ccgctcccgt caggatcctg acggaggggt
     2581 tgacgttccg caccgcggta acagtccccg tgatgatctc gggattcgcc tgggaaactg
     2641 agactgtccc gccgatcagc tcggggggtt cgatcgcgac gtaatcaggc ccgagcgagg
     2701 cagctgcagc gctggtggcg acattattcg agcagactac cgaaatgagc cctgcggacc
     2761 ggagcgccga gaccgccgca tcgatatctg ccagggtgag gcggtgctcg gaatggttca
     2821 cgagggagcc aaccgctccc gcggacctga tcccctcgac agtcacagag cctgtgtggg
     2881 cgccaggcgc agcagcatcc acatgctggg cgtacacggg aagcgagtag tgcatagaca
     2941 tcgggtggat gttcatgtag gtt
//
LOCUS       DAOQVJ010000169         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39218_length_2955_cov_4.342414, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000169 DAOQVJ010000000
VERSION     DAOQVJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39218_length_2955_cov_4.342414"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKL26_04535"
     CDS             complement(<1..474)
                     /locus_tag="PKL26_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS82995.1"
                     /translation="MVLCLFLVLPASAALGVNGIDPSSDLNTGTVFITNLSGTDFPDQ
                     GYLQVALNRTGYAEIAGQFVTVASPTWATCVFDLTDEIAGDWNVAVTNTTSGEKVVSP
                     VLFTVMNLPPEVIDIDPDEEENTGVAHITNLSGANFRGPANVTLSLAGDVIEATDV"
     gene            complement(997..2034)
                     /locus_tag="PKL26_04540"
     CDS             complement(997..2034)
                     /locus_tag="PKL26_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNS82996.1"
                     /translation="MVRDLLTKLSNAHGISGSEGSITGIVRKELKKYVDEIREDRMGN
                     LIAVKKGNNFKVMLAAHADEIGLMVKSIDEKGFLRFVALGGWFAPTLYNQRVVLHGSK
                     GRVFGVLGGKPPHKMDEEERKKVVKVEDMFIDIGACSREEVAEMGVGVGTPVSVDREV
                     APLANGRLTGKAFDNRVGVAMLVRTMQEVKSPFTIYAVFTVQEEVGLKGAKTSAYTLQ
                     PDCAIATDVTIPGDHPGIDQKDVPVEMGKGPIITIVDASGRGLIASRAMVKWLCDTAK
                     AHDIGVQLEVGSGGTTDATAIHLERGGIPSTTLSIASRYIHSPVEVVDVRDIEKGVRL
                     LVNALQTKPKF"
     gene            complement(2041..>2955)
                     /locus_tag="PKL26_04545"
     CDS             complement(2041..>2955)
                     /locus_tag="PKL26_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS82997.1"
                     /translation="IPELREALSSKFRNENGLSYSADQILVTAGASEALHIIMHALVE
                     EGDRVLYADPGFVSYGALAVLAGARPEGVPLDRDLRIDVEAAKKMMDGARLFVLNSPG
                     NPTGAVETKENIRALVEYGDDRGVTIVSDEVYEHFIYGATHWSAARFGEDVITVNAAS
                     KTYAMTGWRLGYLAGPKEYVEQCLKVHQYCQACATSISQYAALTAYTGPQECVEEMRR
                     EYQARRDYLCGELARMGFSFPVPEGAFYLFAPLGRGLVERILKKGVIIVPGEAFGCNA
                     PEYARMNYATSRENLKAAVERIREAMEG"
BASE COUNT          586 a          922 c          763 g          684 t
ORIGIN      
        1 cacgtccgtg gcttcgataa catcgccagc cagggagagg gtgacgtttg ccggcccgcg
       61 gaaattggcc cccgagaggt tggtgatatg cgccacaccg gtgttctctt cctcgtcggg
      121 gtcaatgtca ataacctctg gcgggaggtt catcacggtg aagagaactg gggagacgac
      181 cttctcacca ctggtggtgt tggtcactgc cacgttccag tcccctgcga tctcgtcagt
      241 gagatcaaag acacaggtcg cccaagtcgg ggaggccact gtcacgaact ggccggcaat
      301 ctcggcataa ccagtcctgt tcaacgcgac ctggagatat ccctggtcag gaaagtccgt
      361 tccagagagg ttggtaatga atacagtccc tgtgttcagg tccgaagatg ggtcgatccc
      421 gttcacgcca agggcggcgc ttgcgggaag caccaggaat agacacagga ccataggtat
      481 cgctatctgc agcccttttt cgcacttcat acgggtacct cagttgcatt gttgtataca
      541 ttccccaatc gggaattgaa ggtgatatca gacccaaaat tcccgaacaa atgtattcaa
      601 catatcctct tcatgtatat atataaatct ttccctcatc caggccagaa tctggcgtat
      661 tttttcactc ctgctcacat tacatgtcca attttggatt ggggcaatga tcactggtat
      721 tcatttttta gccttgagaa cgctgaatat atctacggtt tgttctcctt ttgatacagg
      781 gtgatctcag ttttattttg atatttcgac aaaaaaaggg agaattaaca actaaaaact
      841 aaaaagcccc gtgctggcgg acaagtggca catacctctt ttcagattcc ccacaaggaa
      901 atatttctat cgtccacatt tcccgcgcgt aatgcccgga tgaagagaaa atgagatttt
      961 taaaaggttc ccggttgccc gggccggcaa tacccgtcag aacttcggct ttgtctgcag
     1021 ggcattgacg aggagccgca cacctttttc gatatccctg acatccacca cctcgacagg
     1081 ggagtggatg tacctgcttg cgatcgagag cgtggtgctg ggtattcctc cccgttccag
     1141 gtggatggca gtggcatctg tcgtgccgcc acttcccact tccagttgca cgccgatgtc
     1201 atgagccttc gcggtatcgc acagccattt caccatcgcc cggctcgcga tcagacccct
     1261 cccgctcgcg tccacgatgg tgatgatcgg ccccttgccc atctccacag ggacatcctt
     1321 ctggtctatc ccggggtggt cgccggggat ggtcacgtcg gttgcaatcg cgcaatcagg
     1381 ctggagcgta taggcgctgg tctttgcacc cttcagcccc acttcctcct gcaccgtgaa
     1441 aaccgcgtag atggtgaacg gggacttcac ttcctgcatg gtcctgacaa gcattgccac
     1501 cccgacgcgg ttgtcaaacg cttttcccgt gagtcggccg ttcgcgaggg gagcgacctc
     1561 cctgtccacc gacacagggg tgccaacacc gacacccatc tccgcaacct cctccctgct
     1621 gcaggcgccg atgtcgatga acatgtcctc cactttcacc accttcttgc gctcctcctc
     1681 gtccatcttg tggggcggct tgccacccag gacaccgaat acccggccct tgctgccatg
     1741 caggaccacc cgctggttgt agagggtcgg tgcgaaccat ccgccgagcg caacaaaccg
     1801 gaggaacccc ttctcatcga tggacttgac catcagcccg atctcgtcgg catgggccgc
     1861 gagcatcacc ttgaaattgt tccctttttt caccgcgatg agatttccca tccggtcctc
     1921 gcggatctcg tccacgtatt ttttcagttc tttcctgacg attccggtaa tgctgccctc
     1981 gcttcctgag atgccgtggg cattcgacaa tttggtaaga aggtccctga ccatgataat
     2041 tcagccctcc atggcttccc tgatcctctc caccgcggct ttcaagttct cgcgggaggt
     2101 ggcgtagttc atccttgcgt attccggcgc attgcacccg aacgcctccc ctgggacgat
     2161 gatgaccccc ttcttcagga tgcgttccac gaggcccctt ccaagggggg caaagagata
     2221 gaacgcgcct tcggggacgg ggaacgagaa gcccatccta gcaagctccc cacagagata
     2281 atccctccgc gcctggtact ccctcctcat ctcctccacg cactcctggg gccctgtata
     2341 cgcggtcagc gccgcatact gggagatgga tgtcgcacat gcctggcagt actggtgcac
     2401 cttcaggcac tgctctacgt actccttcgg ccctgcaagg tatccgagcc tccagccagt
     2461 catggcatat gtcttgctgg ccgcgttcac cgtgatcacg tcctccccga aacgagcggc
     2521 gctccagtgc gtggcaccgt agatgaagtg ctcgtatacc tcgtcgctca cgatggtcac
     2581 cccccggtcg tccccgtatt ccacaagggc cctgatgttc tcctttgtct ccaccgcgcc
     2641 ggtggggttc cccggagagt tcaggacgaa gaggcgggca ccgtccatca tctttttcgc
     2701 cgcctccacg tcgatgcgaa ggtctctgtc caggggcacc ccttcgggcc gcgcgccggc
     2761 gagcacggcc agggctccgt acgagacgaa gcccgggtca gcatacagca ccctatcgcc
     2821 ctcctccacg agggcatgca tgattatgtg gagggcttcg ctcgcacccg ccgtgaccag
     2881 gatctggtca gcggagtaag agagcccgtt ctcattcctg aacttcgagg agagcgcctc
     2941 gcgcagttcc ggtat
//
LOCUS       DAOQVJ010000170         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39443_length_2943_cov_3.554363, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000170 DAOQVJ010000000
VERSION     DAOQVJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39443_length_2943_cov_3.554363"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKL26_04550"
     CDS             <1..363
                     /locus_tag="PKL26_04550"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNS82998.1"
                     /translation="PTMEEAISGMNEIAPEHLSIQVADALAVLNAVRHAGSIFVGPYT
                     PVAAGDYASGTNHVLPTAGYAKTYSGLNVSHFCKTSTVQIIDREGLDAIGDVIEAIAD
                     AEGLNAHAESVRIRRSFN"
     gene            complement(567..1433)
                     /locus_tag="PKL26_04555"
     CDS             complement(567..1433)
                     /locus_tag="PKL26_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNS82999.1"
                     /translation="MRAPTRLRELIAADPVRVIPVVHAPLCARIAGQAGFSAIFSAGY
                     ANSAALLGRPDVGLLTLTEMVDCASRIVDATTIPVFADGDTGYGNVTNVVRTVSLYEK
                     AGVGGVFIEDQVSPKRCGHMEGKALIPAEEMAAKVRAAVDAKVDPDLVIMARTDAIAV
                     NGIEDAIERANLYYEAGADLTFVEAPESVEQMRRICREVNGPAFANMLPGGKTPVLSA
                     PELEEIGYAVVADPVSCTYAITHAVRHVFSELKRTGSSGSYPGHLVGFDEFNRLVGLQ
                     EIREREQSYYRK"
     gene            1572..2864
                     /gene="rbcL"
                     /locus_tag="PKL26_04560"
     CDS             1572..2864
                     /gene="rbcL"
                     /locus_tag="PKL26_04560"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HNS83000.1"
                     /translation="MAIDWYMDFVDKGYEPAKDELDALFYFEPAAGISSEEAAGRIAS
                     ESSTGTWTTLHALPPRMRDLQATVYEIDGRYVRIAYPQGLWEEGNAAQLLSGIAGNIF
                     GMKAIKNLRLIDVSLPQGYIRHFKGPHFGMEGIRKMMRIEGRPLTGAVPKPKVGFSAR
                     EHAEIGYETWMGGFDFVKDDENLTSTSFNRFEDRVREMTRMRDRAEKATGEEKSAFLN
                     ITGETETMKERAKMLADSGWNYAMIDVVVAGTAAVSTLRDYCSDLGLAIHAHRAMHAS
                     FDRNPKHGITMQFLAKMMRLIGVSQIHTGTAVGKLTGSKKEARILADVLRERRVKEVR
                     HALLAQDWGSMKSAFPVSSGGLHPGLVPAVLDIYGTDLVLLVSGGIHGHPKGTRAGAI
                     AAMQAIEAWQDGISLEEKAKSSPELAQALEKWGYYRPK"
BASE COUNT          583 a          903 c          926 g          531 t
ORIGIN      
        1 cccaccatgg aggaggcgat ctccggcatg aacgagattg cacccgaaca cctctcgatc
       61 caggtcgccg atgcgctcgc ggtcctcaac gcggtcaggc acgccgggtc catcttcgtc
      121 gggccgtata cccctgttgc ggccggcgac tacgcatccg ggacaaacca cgtcctgccc
      181 acggcagggt acgcgaagac ctactcgggg ctgaacgtct cgcacttctg caagacctcg
      241 acggtccaga tcatcgaccg ggagggcctt gacgccatcg gcgatgtcat cgaggcaatt
      301 gccgacgccg aggggctcaa tgcccatgcc gagtctgtgc gcatcaggcg ctcattcaac
      361 tgaataaccc ctcttctttc ttgcgtgcgc acctcttttt cggacaccgg accgggaggg
      421 agtcgggaat gtataaggac ccaatacaat ctccgatacc tgccttgtca ctgaagtgta
      481 attggggccc gtgatcccga aaagcgtgcg gatcgtccgg aactttctgc aggcacccgg
      541 atggggatat ccctgtcgaa gaaccgctac ttcctgtaat acgactgctc gcgctcccgt
      601 atctcctgga ggcccaccag gcggttgaac tcgtcgaacc ctaccaggtg cccgggatac
      661 gatccagagg aaccggtcct cttcagttcc gagaagacgt gccggactgc gtgcgtgata
      721 gcatacgtgc aggagacagg gtctgcgacg acggcgtacc cgatctcttc aagttcggga
      781 gcggagagga ccggggtctt tccgccaggg agcatgttgg caaacgccgg gccattgacc
      841 tccctgcata tccgccgcat ctgctccact gactcggggg cctcgacgaa cgtgaggtcc
      901 gcgccggcct catagtagag gttcgccctc tcgattgcat cctcgatgcc gttcacggca
      961 atcgcatcgg tccttgccat gatcacgagg tccgggtcca cctttgcatc gactgcggcc
     1021 ctgaccttcg ctgccatctc ttctgccggg atgagtgctt ttccctccat gtgaccgcac
     1081 cgcttcgggg agacctggtc ctcgatgaag actcccccga cgcccgcttt ctcatacagg
     1141 gacacggtac ggacaacgtt cgtcacgttc ccgtatcccg tgtcgccatc cgcaaagacc
     1201 gggatcgtcg tcgcgtccac aatcctcgag gcgcagtcga ccatctcagt gagcgtgagc
     1261 agcccgacgt cgggcctgcc caggagcgcg gcggagttgg cgtagcccgc cgagaatatg
     1321 gcggagaacc ccgcctgccc ggcgattctc gcgcagaggg gggcgtggac aacggggatg
     1381 acacgaacag ggtctgctgc gatcagttcg cggaggcggg tgggggctct cattggtttt
     1441 cctcaccagg agtaaaaacc ggccgggctt attcttttcg ccggaatgga ccgtgtgggc
     1501 cttgccgggc ctgcaccatc ttcatcctta ttatgcatcc cctcacctaa ctctacaggg
     1561 gatttttgaa aatggcgatc gactggtaca tggacttcgt ggataagggg tatgaacccg
     1621 caaaggacga gctggacgca ctcttttact tcgagccggc agccgggatc agcagcgagg
     1681 aggcggcagg gagaatcgcg tccgagagct cgaccgggac ctggaccacg ctccacgcgc
     1741 tcccgcccag gatgagggac ctccaggcca cggtgtacga gatcgacggg agatacgtgc
     1801 ggatcgcgta tccacaaggc ctctgggagg aggggaacgc cgcccagctc ctgagcggga
     1861 ttgcagggaa catcttcggc atgaaggcga tcaaaaactt aaggctcatt gacgtgagtc
     1921 ttccgcaggg gtatatcagg cacttcaaag gcccgcattt cgggatggag ggcatccgga
     1981 agatgatgcg gatagagggc cgcccgctga ccggcgcggt ccccaagccc aaggtaggtt
     2041 tttccgcacg agagcatgcc gagatcgggt acgagacctg gatgggcggc tttgacttcg
     2101 taaaagacga cgagaacctc acctcgacat cctttaaccg gttcgaggac cgcgtccggg
     2161 agatgaccag gatgcgtgat cgggccgaga aggccacagg tgaggagaag tccgccttcc
     2221 tcaacatcac gggagagacg gagacgatga aggagcgtgc gaagatgctc gcggacagcg
     2281 ggtggaacta cgcgatgatc gacgtggtgg tggcagggac tgccgcggtc tcgacgctcc
     2341 gtgactactg ctcggacctt ggcctcgcta tccacgccca ccgggcgatg cacgcgtcat
     2401 tcgacaggaa cccaaagcac gggatcacca tgcagttcct ggcaaagatg atgcgcctga
     2461 tcggcgtctc ccagatccat accgggaccg cggtgggcaa gctcaccggg tcaaagaagg
     2521 aggcccggat cctcgccgac gtgctcaggg agcgccgcgt aaaagaggtc cgccacgccc
     2581 tcctcgccca ggattggggc tccatgaaga gtgcattccc ggtctcttcc gggggcctgc
     2641 acccggggct cgtccccgcg gtcctcgaca tctacgggac agacctcgtg ctcctcgtga
     2701 gcggcgggat ccacggccac ccgaagggga cccgtgcggg ggcgatcgcg gcgatgcagg
     2761 ccatcgaggc ctggcaggac gggatcagcc tcgaggagaa ggccaaatcg tcgccggaac
     2821 ttgcccaggc tctggagaag tggggatact acaggccgaa atgagtgcgg ggatatcacg
     2881 gatgcgatcc tggccgggat agcccgccac gcatcaaata tggggggagg ggcgcccttt
     2941 ttt
//
LOCUS       DAOQVJ010000171         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39667_length_2933_cov_4.026755, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000171 DAOQVJ010000000
VERSION     DAOQVJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39667_length_2933_cov_4.026755"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1861)
                     /locus_tag="PKL26_04565"
     CDS             complement(152..1861)
                     /locus_tag="PKL26_04565"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNS83001.1"
                     /translation="MTSRPPDLEYTVDEVPPPPTSFILALQHVGIVTTAFVFPILVAR
                     AASLDAGATAFLVSMSMLANGISTIFQAFRLRNFGSGYLIPRVAGPNFVSASILALNT
                     GGLPLLAGMTAISGLFQSALSTVVHRLRVLFPVEVTGLVIMMLGVAVVPVGLPSFFGM
                     STYDAAPSGIDTAIAFVVLGLTIGITVWGRGQLRFIPLIVGMGAGYLLCLAAGLVSPE
                     QVALVAAAPVVALPDLRYLGFAFDPVLLIPFFIAALTTFVKSVGEISICQRINDPSWK
                     RPDMKNIRSGLFADGIASSIGGMFGGMGQTGSSSNIGLSIATRATSRYIGYVTGALLI
                     GLAFLPMFASIFVMMPKPVIGATLLYVAGFIIVGGFQTITTRMLDSRKIFVIGLSFIF
                     GISIYILPGAYRGVPPIFKPIFDSALALTTVLAIVLNLILRIGISKTHEFTIDSKTPV
                     YETISLTMERQGEAWAARKEEIDRMAGALTESCEVLVHQGYATGPVRILVRFDEFNLD
                     STVSYTGRPLPLPDQSPSHEFVLESPEGLAHLAGYLVRTRSDRVTSSCEGTRCTLQIH
                     NEH"
     gene            2141..>2933
                     /locus_tag="PKL26_04570"
     CDS             2141..>2933
                     /locus_tag="PKL26_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNS83002.1"
                     /translation="MVTESIRRYQEIVRILSKYGFGAFFLEDISPGLAKMDLQKRLHP
                     ELEGYTKYELMRFAIEELGPAFVKFGQILSTRREMIPPEVYEELIMLQDKVAPLSYEV
                     IEPTIVKYCGPVEEAFLSFERVPFAAASVSQVHRAVLPDGTKVAVKVQRPGIRRKIEV
                     DLPIMRRLAERVEKLEPDLVVYNPTGLVDEFVTQIYKELDFTQDGQNADTLNRNFKDN
                     PKVKAPKIYWEYSGTQLLTMEYIEGVRIDNVAAIREMGVNPADIAE"
BASE COUNT          717 a          773 c          890 g          553 t
ORIGIN      
        1 tctgtttctt gtccaacccg cagcattcga acattctccc gccacccgtc attcattgca
       61 aaccgtgggg gaagtatgag acactttttg catccaacaa gcctccgatg cgataaacga
      121 gggcagcacc ctttggttac gtatcctcag atcagtgctc gttgtgaatc tgcagagtgc
      181 aacgcgtgcc ttcgcatgat gaagtgacac ggtcagaccg agtcctgacg agatacccgg
      241 cgagatgggc gagcccttcc ggtgattcca gcacgaactc gtggcttggg gactggtcag
      301 ggagaggaag cggtcttccc gtataggaga cggtactgtc gaggttgaac tcgtcgaacc
      361 tgacaagaat cctgaccggt ccggtcgcat aaccctgatg gacaagcacc tcgcaggatt
      421 cagtcagggc acccgccatc ctgtcgatct cttccttcct cgcagcccat gcctcaccct
      481 ggcgctccat cgtaagagag atggtctcgt agacaggggt cttggaatcg atggtaaact
      541 cgtgagtctt tgagatcccg atacggagaa tgagattgag aacaatggcg agtacggtcg
      601 tcagggcaag ggcagagtcg aagatgggct tgaaaatggg ggggacaccc ctgtatgcac
      661 ctgggaggat atagatgctg atcccgaata tgaaagagag gccgatgaca aatatcttgc
      721 gggagtcaag catcctcgtt gtaatcgtct ggaacccccc gacgataatg aacccggcaa
      781 catagaggag tgttgcccct atgacgggct taggcatcat cacaaagata ctggcaaaca
      841 tcggcagaaa cgcaaggccg atgagcagtg ccccggtgac atacccgata tacctgcttg
      901 tagcccgcgt agcaatcgag aggccaatat ttgaggatga ccctgtctgg cccataccgc
      961 cgaacatacc tccgatggac gaggctatcc cgtctgcgaa gaggccagaa cggatattct
     1021 tcatgtcagg gcgcttccac gagggatcgt tgatccgctg gcagatggat atctcgccca
     1081 cactcttgac aaaggtcgtc agcgcggcga tgaagaaggg gatgagcagg acagggtcaa
     1141 acgcgaaccc aaggtaacga agatcgggca gggcaacgac tggcgcagct gccacgagtg
     1201 cgacctgttc ggggctgacg agcccggctg ccaggcagag gaggtacccg gcacccatgc
     1261 cgacgatgag tggaatgaac cgtaactgcc ccctgcccca gaccgtgatc ccgatggtga
     1321 gtccgaggac gacgaatgca atcgcggtat caattccgga aggcgctgca tcgtacgtgg
     1381 acatgccaaa gaagctcgga agtcctacag ggactacggc aacgccgagc atcatgatga
     1441 caaggcctgt cacctcgacg gggaagagta cccgcagccg gtggacaaca gtcgagagag
     1501 ctgactggaa gaggcctgag atggcggtca ttcccgcgag gaggggaaga cccccggtat
     1561 tcaaggcgag gattgaggcg gacacgaagt ttggccccgc cacccgcggg atgaggtagc
     1621 cggatccgaa gttgcggaga cggaacgcct ggaagatcgt cgatattccg ttggcgagca
     1681 tggacatcga gacaaggaat gcagtcgccc cggcatcgag actggctgcc cttgcaacga
     1741 ggatgggaaa gacaaaggcg gtggtaacta tcccgacgtg ctggagtgcg aggatgaaag
     1801 aggtcggggg aggcgggacc tcgtcaaccg tgtattccag gtcaggaggc cgcgaggtca
     1861 cacggaacga ttggcggaca atccgatgaa tgttgtgaat cggatgctag ggcgccatct
     1921 ccctcgtggt gtgtgttccg gcacccggga atccgttgaa gcaggggcat gaccatgcat
     1981 accagcgctg aagggaatcc ggcaggatgc ccgggttaat tcccgggcct gccacccgga
     2041 ttcccggtca cgaaacggca cgcacggacc ccttcctctc aatgaaccgg gagcgcgggt
     2101 gttgtccatt ttatttacat gaaaaaccga atatccctgc atggtgaccg agagcatccg
     2161 ccgataccag gaaattgtca gaattctctc aaaatatgga tttggcgctt tttttctgga
     2221 ggacatctcc cccggactcg caaagatgga cctccagaag cgtcttcatc ccgagctaga
     2281 agggtataca aagtacgaac tgatgcggtt tgccatcgag gagcttggtc ctgcatttgt
     2341 caaattcgga cagatcctca gcacgagacg ggagatgatc ccgcctgaag tctacgagga
     2401 actcatcatg ctccaggaca aggttgcccc gttatcatac gaggtaatcg aacccaccat
     2461 cgtgaagtat tgcggcccgg tggaggaggc attcctgtct tttgagcggg tgcccttcgc
     2521 agcagcctcg gtgtcccagg tgcaccgggc ggtgctcccg gacggcacga aggtggcggt
     2581 caaggtccag aggcctggca taagacggaa gatcgaggtc gacctcccga tcatgcgaag
     2641 gcttgcggag cgggtcgaga aactcgaacc cgatcttgtg gtctacaatc ccacggggct
     2701 cgtggatgaa tttgtgacac agatctacaa ggagctcgat tttacgcagg atggccagaa
     2761 tgccgatacc ctgaaccgga atttcaagga taacccaaag gtcaaggcgc caaagatcta
     2821 ctgggagtat tcggggacac aactgctgac aatggagtat atcgaaggcg tgaggattga
     2881 caatgtcgca gcaataaggg agatgggcgt gaacccggcc gatatcgccg aga
//
LOCUS       DAOQVJ010000172         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39763_length_2929_cov_3.422408, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000172 DAOQVJ010000000
VERSION     DAOQVJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39763_length_2929_cov_3.422408"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKL26_04575"
     CDS             <1..556
                     /locus_tag="PKL26_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNS83003.1"
                     /translation="AIVKGLPLSYHRDLQELNPHLWRGIRDTKHSTRLLVDMLSSATF
                     NTAVMAGEAGKGFSTATDVADMLVQSHGFAFRTAHTLVGKAIQKGALDIPALDQAAEE
                     MGIQPPSKMGIREEEIAAALDVRACIERRRAPGSPSPFAVRISLSSRRTALHEDLRSL
                     EERMAHYAESVRRLISDAKKEVGK"
     gene            553..1788
                     /gene="gatD"
                     /locus_tag="PKL26_04580"
     CDS             553..1788
                     /gene="gatD"
                     /locus_tag="PKL26_04580"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNS83004.1"
                     /translation="MSGFLPGDLVNCTVGGTGRRGTYITDRDGMAVLKMDSGYNIGVF
                     PGDCTLIERPSPLRVERAEVEQDPSLPLISIVSTGGTIASRIDYRTGAVTSQFDAADI
                     LRAIPGLSAIARCRTRKLYTILSENMTPSIWQELARAVYEEIQDGARGVIVTHGTDTM
                     AYSAAALSFMLDTPVPVVFVGSQRSADRPSSDNVMNALCSASTAVGDLGEVAVVMHET
                     TSDDRCAIHRGTRVRKMHTSRRDAFQSIGAPPLGFVDYPSLEVKLSPGAVRRGACEPA
                     LHDRLEPSCGLISFFPGMSPDLISSCADVKGLVIAGSGLGHVSTSLIPAIRELIDQGT
                     LVVMTSQCLHGRVCDRVYDTGRDLIRIGVVEGEDMLPETALVKMMWVLGNEPDVERAR
                     CMMQENQKGEYTRRSLHGL"
     gene            1778..>2929
                     /gene="gatE"
                     /locus_tag="PKL26_04585"
     CDS             1778..>2929
                     /gene="gatE"
                     /locus_tag="PKL26_04585"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNS83005.1"
                     /translation="MDYNALGLVAGIEIHQQLNTREKLFCRCPTLLRPFEEHDGEFSR
                     YLRATESELGEIDRAAREEMKNFRRFLYYTYDSTCLVENDEEPPAPLNPEALATCLQI
                     AKMFGMAPIPQVHTMRKLVIDGSNTSGFQRTALVAVNGTLPNGGTIETICIEEEAAQR
                     VKDEVFSLDRLGIPLVEITTSPCMHTPEEVQEIAEYLGMVLRSTGKVKRGLGTIRQDI
                     NISISGGARVEIKGVQELDLIAEVVRREVQRQERLLSIRDRLKERGASVWGTPVDVTE
                     IFSHTGSGILKKASRIMAVRLARFGGLVGDEIQPGRRLGSELSDYAKKCGVGGIFHTD
                     ELPAYGVNAEEVTALRDMVGAGESDCIVIVAGTPRQAGCACQQIIRRAEL"
BASE COUNT          619 a          848 c          942 g          520 t
ORIGIN      
        1 cgcaatcgtc aagggacttc ctttgagtta ccaccgagac ctgcaggaac tcaacccaca
       61 cctctggaga gggatacggg acacaaagca cagcacccgg ctccttgtcg acatgctctc
      121 ttctgccaca ttcaatactg ccgtgatggc aggcgaagcc ggaaaaggct tctccaccgc
      181 aacggacgtc gcagacatgc tggtccagtc gcacgggttc gcctttcgga cggcgcatac
      241 ccttgtggga aaggccatcc agaagggggc tctcgatatc cctgcacttg accaggcggc
      301 cgaggagatg ggaattcagc ccccctcgaa gatggggatc agggaggaag aaattgccgc
      361 agcgctcgac gtgcgggcct gcatcgaacg gcggagggct cctggaagtc cctcgccgtt
      421 cgcagtcagg atctctctct cttcgaggag gactgcactc cacgaagatc tccggtcact
      481 cgaagaaaga atggcgcatt atgctgaatc cgtccgccgc cttatcagcg atgcgaagaa
      541 agaggtaggg aaatgagcgg ctttcttccc ggcgacctcg tgaattgcac tgtcggcggc
      601 accgggcgga gagggaccta tattaccgac cgggacggca tggcggtcct gaagatggac
      661 agcgggtaca atataggggt ttttcccggg gactgcacgc tcatagagcg cccttctccc
      721 ctgagggttg agagagccga ggttgagcag gatccgtcgc ttcccctcat ctcgatcgtc
      781 tccacagggg gaacgatcgc aagcaggata gattaccgga ccggggcggt gacgagccag
      841 ttcgatgccg cggacatcct tcgcgcgatc ccgggactca gcgcgatcgc caggtgccgg
      901 acaaggaagc tctacaccat cctctccgag aacatgactc cttcgatttg gcaggaactg
      961 gcaagggccg tgtacgagga gatacaggac ggggcacggg gggtcatcgt cacccacggc
     1021 accgacacca tggcctattc ggcggccgcg ctgagcttca tgctcgatac tcccgtgccg
     1081 gtggtcttcg taggctcgca gagatcggcg gaccgcccca gctcggacaa cgtgatgaat
     1141 gcgctctgca gtgcgagcac tgcggtcgga gacctcgggg aggtggcagt agtgatgcac
     1201 gagactacga gcgatgaccg ctgcgccatc caccgcggga ccagggtcag gaaaatgcac
     1261 acctcacggc gcgatgcgtt ccagagcatc ggggcccctc ccctgggatt cgtggactac
     1321 ccgtcgctcg aagtgaaact ctccccaggt gcggtgagga ggggtgcctg cgagcctgcg
     1381 ctccatgacc ggctcgaacc gtcgtgcggt ctcatctcat ttttccccgg gatgtcacct
     1441 gacctgatca gctcgtgtgc ggatgtaaaa gggctcgtga ttgccgggag cgggctcggc
     1501 cacgtcagca cctccctcat ccccgcgata cgggagctga tcgaccaggg cacgctcgtc
     1561 gtgatgacat cccagtgtct ccacggcagg gtgtgtgacc gcgtctacga cacagggaga
     1621 gacctgatca ggataggcgt agttgaaggg gaggatatgc ttcccgagac ggcgcttgta
     1681 aagatgatgt gggtgctcgg gaacgagccg gatgttgaga gagcacgctg catgatgcag
     1741 gagaaccaga aaggcgagta tacacggagg tcgctgcatg gattataatg cccttggtct
     1801 cgttgccggg atcgagatcc accagcagct gaacacgagg gagaaactct tctgcaggtg
     1861 cccgactttg cttcgaccgt tcgaggagca tgacggcgag ttctcgcgtt acctacgtgc
     1921 gaccgagagc gaactcgggg agattgaccg ggcggcgcgc gaagagatga agaatttccg
     1981 gcggtttctc tattacacct acgacagcac gtgcctcgta gagaatgacg aggagccgcc
     2041 cgcacccctc aaccccgagg cactcgcaac ctgcctccag attgcaaaga tgttcgggat
     2101 ggccccgata ccacaggtgc acactatgcg gaagctcgtc atcgacggct ccaacaccag
     2161 cggcttccag cggactgcac tggttgccgt gaacggaacg cttccgaacg gggggaccat
     2221 agagacgatc tgcatagagg aggaggctgc ccagcgggta aaggacgagg tcttctccct
     2281 tgaccgcctc ggtatccccc tggtggagat taccacttca ccctgcatgc acacgcccga
     2341 agaggtacag gagattgccg agtacctggg aatggtgctc cgctcaacgg ggaaagtgaa
     2401 gcggggcctg gggacgatcc ggcaggatat caacatctcg atcagcggcg gcgcccgcgt
     2461 agagatcaaa ggcgtccagg aactggacct catcgcagag gtggtacgga gggaagtgca
     2521 gaggcaggag cggctcctct ccatcaggga ccgcctgaaa gaacgcggcg cttctgtttg
     2581 ggggactcct gtggatgtca cggagatctt ttcccacaca gggtccggga tcctgaagaa
     2641 ggcctcccgg atcatggccg tcaggctcgc acggtttggc ggccttgtcg gggatgagat
     2701 ccagccgggc aggaggcttg gcagcgagct ctcggactat gcaaagaagt gcggggtggg
     2761 tggcatcttc cacaccgacg agcttcctgc ctacggcgtg aacgcagaag aagtgaccgc
     2821 gcttcgggat atggtcggtg caggagagtc ggactgcatc gtcatcgtgg caggaacccc
     2881 ccgacaggcc gggtgcgcct gccagcagat catccgaagg gcggaactg
//
LOCUS       DAOQVJ010000173         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_39897_length_2923_cov_4.887727, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000173 DAOQVJ010000000
VERSION     DAOQVJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39897_length_2923_cov_4.887727"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..905)
                     /locus_tag="PKL26_04590"
     CDS             complement(30..905)
                     /locus_tag="PKL26_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HNS83006.1"
                     /translation="MYVGIDHGTSAIRFSAGDGRRFKVRREDAVSFRIQDLSRLAPLD
                     EIDGIALTYSMGDNFSAITPIRDLENRGLVSREGAGKHTGGGTRVFDQVAGSSIPAVA
                     IPGLHRRSPTDPRFSVYSHQASPEKIGIAFRVVQDLGGDVVISDVSSNTVSILVRDRR
                     LVGALDACIFAPGTEHGALDVDAIRRVDRREMSANEAFLHAGVNHSLSPERRIPALAL
                     FSAMECASLLLLAPGARVALAGTMAPVVAGEVRSLLRTDVAVYDEWCAAGGLALIAGE
                     VFSGARDILGLEVHC"
     gene            complement(980..1864)
                     /locus_tag="PKL26_04595"
     CDS             complement(980..1864)
                     /locus_tag="PKL26_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNS83007.1"
                     /translation="MVSRIDAPQGGLVFFKGVLMGMCDIIPGVSGGTIALITGIYERL
                     ISAIGSIGISPLRALIRGRREEAVDAIHRMDPVFLVVLMAGIGAGLLAMSRIVLSVLQ
                     AFPAETYAFFFGLIVASSVLIAYRIERWDTVIATVWVGLGLAAGYLIGGLGQSGLGHS
                     LPMLFVTGFVALCAMILPGISGAYLTLVMNQYEYLLSAIATLDIVPLLAYIAGGAAGI
                     ISMSRILKIILRTYHTQSLAFLTGLMLGSTRMMYDHIVAAGGTIASVLVAGVAGVLLI
                     ALIELALRLFSKRSPDPE"
     gene            1921..2868
                     /locus_tag="PKL26_04600"
     CDS             1921..2868
                     /locus_tag="PKL26_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNS83008.1"
                     /translation="MKAVVTGGAGFIGSHLVDALVTRGDEVCVIDNLSTGDLRHIAGH
                     IDSGAVRFVRGDLLEEGWQSSIRDANRVYHIAADPDVRASAGNPAGVYARNVTTTARV
                     LEAMREAGCREIAFTSTSTVYGEADIIPTPEDYPGMRPISIYGATKLACEAMISGYAY
                     TYGWNSWVFRFANIIGERSNHGVIWDFVHKLRKNPREMEILGDGRQAKSYCSVSACVD
                     AVLFAVTHAHEPFACFNIGTEDWIDVTSIASIVAAEMGLERVSFRYTGGDRGWVGDVP
                     RMLLSVERLKSLGWRPLVTSETSVREAARALIRESGHFP"
BASE COUNT          638 a          866 c          874 g          545 t
ORIGIN      
        1 cgaattcgtc ctgcaagggg acccggcact cagcagtgca cttcgaggcc caggatatcc
       61 ctggcaccgg agaagacctc tcctgcaatc agggcaaggc cacccgcagc acaccattcg
      121 tcatagactg caacatctgt tcggaggaga gatctcacct cgcccgccac cacgggggcc
      181 attgtccccg caagggcaac gcgggctcca ggcgcgagga gaaggaggga ggcgcattcc
      241 atcgcagaaa aaagcgcgag tgcgggaatt ctcctttccg gtgagagcga gtgatttacc
      301 cctgcatgca ggaacgcctc gttcgcactc atctcacggc ggtccaccct ccggatggcg
      361 tccacgtcaa gcgcaccgtg ctcggtgcca ggagcaaaga tgcacgcatc gagggctccc
      421 accagtcgcc ggtcccgtac caggatgctt acggtgttcg aactcacatc agagatcacc
      481 acgtcgccgc cgaggtcctg gaccaccctg aacgcaatac caatcttttc cgggctggcc
      541 tggtgggaat agacagagaa gcgcgggtcg gtgggcgatc tcctgtggag ccctggtatg
      601 gccacggcag ggatgctgct ccctgcgacc tggtcaaaga cccgggtccc accccccgtg
      661 tgcttcccgg ccccctccct gctgacaagg cctctgttct cgaggtcccg aatgggagtg
      721 attgcagaga aattgtctcc catcgagtaa gtgagggcta tcccgtcaat ctcatcgagc
      781 ggggccaggc gggagagatc ctgtatccgg aacgataccg cgtcctctct cctgacttta
      841 aaccgccggc cgtcacctgc agagaaccgg atcgccgagg tcccgtggtc gattccgacg
      901 tacatctcaa tatctctttg cgcgcggcga atttatcatt gtgatccggc aggagctttc
      961 ccggtcttct ggtcgcatgt tattcgggat cggggcttcg tttcgagaac aggcggagag
     1021 ccagttcgat aagcgcaatc agcagcaccc ccgcaacccc tgcaaccagg acagatgcga
     1081 ttgtcccgcc tgcagccaca atatggtcgt acatcatcct tgtcgagcca agcatcaacc
     1141 cggtgaggaa cgcgagcgac tgcgtgtggt aggtgcgaag gatgatcttc aggatacggc
     1201 tcattgagat gatgcctgcc gcacctcccg cgatataggc gagaagaggg acgatgtcaa
     1261 gcgtggcgat tgccgagagc agatactcgt actggttcat gacaagggtc aggtacgcac
     1321 ctgatatgcc cgggagaatc atcgcacaca gggcaacaaa accggtgaca aagagcatcg
     1381 gcagtgaatg gcctagccct gactggccaa ggcccccaat caaatacccg gcggcaaggc
     1441 ccagccccac ccataccgtt gcgatgacag tatcccaccg ttcgatccgg tacgcgatga
     1501 ggaccgatga ggcgacgatc aacccgaaaa aaaacgcata tgtttcagcg gggaatgcct
     1561 gcaggaccga gaggacgatc cttgacattg caagcaagcc ggcgccaatc cctgccatga
     1621 gcacgacaag gaacaccggg tccatgcggt gtatcgcatc aaccgcctcc tctctcctgc
     1681 cgcgaatcag cgcgcgtagc ggagatatcc cgatacttcc aatcgccgat atcagtcgct
     1741 cgtaaatccc ggtgatgagc gcaatcgtgc cacccgagac cccggggatg atgtcgcaca
     1801 tgcccatgag cacccccttg aaaaagacaa ggccgccctg tggcgcatcg attctggaaa
     1861 ccacaccttt agtatgggag agagacgttt aatattgatt acacggggaa gggaaacaac
     1921 gtgaaggcgg tcgtcacagg aggtgccggg ttcatcgggt cacacctcgt ggatgccctg
     1981 gtcacccggg gggatgaggt atgcgtcatc gacaacctct caacagggga cctccggcat
     2041 atcgcggggc atatcgattc cggcgcggtc aggtttgtca ggggagatct cctcgaagag
     2101 ggctggcaga gctctatccg ggatgcgaat cgtgtctatc acatcgctgc agacccggat
     2161 gtcagggcca gtgccggtaa cccggcaggg gtgtatgccc ggaacgtgac gacaacggcc
     2221 agggtgctcg aggccatgcg ggaggcaggg tgcagggaga tcgcattcac ctctacctca
     2281 acagtgtatg gcgaagccga catcatccct acgcccgagg attacccggg tatgcgaccc
     2341 atctccatct acggcgcgac aaaactcgcc tgcgaggcga tgatctcggg ttacgcctat
     2401 acatacggct ggaattcgtg ggtgttcagg ttcgcaaaca tcataggcga gaggagcaac
     2461 catggcgtca tctgggattt cgttcacaag ctccgcaaga atccgcggga aatggagatc
     2521 ctcggtgatg gtcggcaggc gaagtcctat tgctcggtgt cggcctgcgt ggatgcggtc
     2581 ctgtttgccg tcactcatgc acacgaaccc tttgcctgct tcaatatcgg gacggaagac
     2641 tggatcgacg tgacctcgat cgcgtccatt gtggcagcgg agatggggct tgagcgcgtg
     2701 tccttccgat acacaggcgg cgatcgcggg tgggtggggg acgtcccccg tatgctcctc
     2761 tcggttgaaa ggctcaaatc gctcggctgg aggcccctgg tgacttcgga gacatctgtc
     2821 cgggaggctg cccgggcgct gatccgggaa tcgggtcact tcccctgaac cgggcgatac
     2881 aggcaggaca gacatgcaga agaagtatat cgttattctc gga
//
LOCUS       DAOQVJ010000174         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40091_length_2913_cov_4.770119, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000174 DAOQVJ010000000
VERSION     DAOQVJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40091_length_2913_cov_4.770119"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKL26_04605"
     CDS             <1..237
                     /locus_tag="PKL26_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNS83009.1"
                     /translation="SSIIHYPLHEGMAECPRAVLGMFDPSARPCVPVDMLTMAIPMRL
                     FGQIVESMEESFLITDTWEKVKKRIEMSEPRNPR"
     gene            complement(346..1554)
                     /locus_tag="PKL26_04610"
     CDS             complement(346..1554)
                     /locus_tag="PKL26_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS83010.1"
                     /translation="MRGIILQLSLRNIRIHFLRSVLAALGIVIGVVAISSMGMMGANM
                     TLSVTEELSGMANNLVVRADTGGGGFGGGPGGGGGSGNGDESISEEEFREITKIAEKY
                     GLVYGVQSESDRIEVGDRRGRSTVFGLDDTVIPTLFTLSEGSFPKSTSSVVIGPTLAN
                     RFDLKTGSRIDIGDPDEGETTTVRIVGILEERGMSMDVNSDSAIIANQKLFNGIYGKE
                     GEYDQVNIILNDINDTARVKSDILDKMNRKEETVTIQDSSRMLESITSTLSTMTTFVM
                     AIAGISLLVAATSIFNVMMMSVTERVREIGIMRSIGTQKSEVRKMFIYEAVILGAIGA
                     VIGAVLSLAIGWIVVLAMVGSTDYFFSPASLVYIPSAMSVGIVICVASGVYPAWRASN
                     LDPIEALRAE"
     gene            complement(1551..2225)
                     /locus_tag="PKL26_04615"
     CDS             complement(1551..2225)
                     /locus_tag="PKL26_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS83011.1"
                     /translation="MDEKPLIELAGVTKVYPLPSGDVVALDRVSLTIEDGEFVAIMGP
                     SGSGKSTLLNHLGCLDRPTSGDLYIRGRNTREMNDRELTELRLNTIGYIFQRFNLIPL
                     LTAYENVEYPFLLKHRKPDDTGWVDSLLGMVGIDRAMAAHKPPELSGGQQQRVAIARA
                     LVNDPMLLLCDEPTGNLDSVTGHQIMEVLSELNEKGRTILMVTHDPEVAKCARRTIVM
                     RDGRVA"
     gene            complement(2233..>2913)
                     /locus_tag="PKL26_04620"
     CDS             complement(2233..>2913)
                     /locus_tag="PKL26_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83012.1"
                     /translation="GTLEPDEEKKATFEITAERDTTMAFDVSYRNGNNLHHLVLEMPV
                     TVGEGGTAAEMVVNNVEVVQAGSTFTLSGDVTNAGLRDAKSVKVTVDSPAVPVDPNPV
                     YVIGALEPDDFSSFEVTCTVRDVSSIPLIIQYRDKDGKVFEDRVNISIRSTAVPASGE
                     ASGASQGLPSGNTRRPGGMLGFGNGISKIPFTEILIVIAACIAVAVAWRKGYLGKAVD
                     IVRKRSGR"
BASE COUNT          610 a          878 c          744 g          681 t
ORIGIN      
        1 agctccatca tccattatcc cctccatgag gggatggcag agtgtccgag agcggtgctc
       61 gggatgttcg atccatcggc gcgtccctgc gttccggtcg acatgctgac aatggcaatt
      121 cccatgcgct tgtttgggca gattgtcgaa tcgatggagg aaagcttcct gatcactgat
      181 acatgggaaa aggtgaagaa gaggatcgag atgagtgaac cgaggaatcc ccggtaaaag
      241 ggaaatccct ccttccaagg gatcctatct cccacgcctg atggcgcgcg gattgtctgc
      301 atcaccaggt cggcaatgaa tggatatgag ggaatccctt cccactcatt ctgccctcaa
      361 tgcttcgatg ggatcgaggt tcgatgcccg ccatgcggga tagacaccgg atgcaacgca
      421 tatgacgatg ccgaccgaca tggcagacgg gatatagacc aggcttgcag gtgaaaagaa
      481 gtagtcagtg ctccctacca tcgcaaggac gacgatccac ccgatggcaa ggctgagcac
      541 ggcgcctatg acagccccga tcgcccccag tatcaccgct tcgtagatga acatcttcct
      601 gacctcggac ttctgggtgc cgatgctccg catgatgccg atctccctga ccctctctgt
      661 caccgacatc atcatgacat tgaagatcga agtcgctgca acaagaagtg agattcccgc
      721 gatcgccatc acgaaggtgg tcatggtcga gagggtcgag gtgatgctct ccagcatcct
      781 gctgctgtcc tggatggtca ccgtctcctc cttcctgttc atcttgtcga ggatgtcgct
      841 ctttaccctg gcagtatcgt tgatatcgtt gaggatgata ttgacctggt cgtactctcc
      901 ttccttcccg taaatcccgt tgaagagctt ctggttcgcg atgattgccg agtcggagtt
      961 gacgtccatc gacatcccgc gctcttcgag gatgccaacg attctcaccg tggtggtctc
     1021 tccttcatcg ggatcgccga tatcgattct gctcccggtt tttaagtcga accggttggc
     1081 aagtgtcggt cctatcacga cagaactcgt gcttttcggg aaagatccct ccgagagggt
     1141 gaaaagcgtg ggaatcaccg tgtcgtccag tccaaagacc gtcgacctcc ccctcctgtc
     1201 tcctacctct atcctgtcag attccgattg aactccgtat acaaggccgt atttctccgc
     1261 tattttcgtg atttccctga attcttcctc cgatattgat tcatccccat tgcctgaacc
     1321 cccgccgccg ccagggccgc ccccgaatcc gccacccccg gtgtcagccc tgaccacgag
     1381 gttgtttgcc atcccagaaa gctcctctgt aaccgagagt gtcatgttcg ctcccatcat
     1441 gcccattgat gagattgcca ctacaccaat tacaatgcca agggccgcaa gcaccgaccg
     1501 caggaagtgg atgcggatat ttctcaaaga gagctgcagg atgatgcccc tcatgccact
     1561 ctcccgtccc gcatgacgat agtccgcctc gcacacttcg ccacttcagg gtcgtgagtg
     1621 accatgagga tggtcctccc cttctcgttc agttcagaaa gcacctccat aatctggtga
     1681 ccggtcaccg agtcgaggtt cccggtcggc tcgtcacaga ggagtagcat cgggtcattt
     1741 acaagggcac gtgcaattgc cacccgctgc tgctgcccac cggatagttc cgggggttta
     1801 tgggcagcca tcgcccggtc tattcccacc atgccgagga gcgagtccac ccagcccgta
     1861 tcatcaggtt tcctgtgttt caaaaggaag ggatactcca cgttctcata cgcggtgagg
     1921 aggggaatca ggttgaatct ctggaagatg tagccgatgg tgttcaggcg gagttccgtt
     1981 aattcgcgat cattcatctc gcgggtattc ctcccgcgga tatagaggtc tccgctcgtc
     2041 ggcctgtcga ggcacccaag atggttcagg agtgtagatt tacccgagcc tgacggtccc
     2101 atgatggcca caaactctcc atcctcgatc gtgagggaga cccggtcgag tgcgaccacg
     2161 tccccggacg gaagcgggta gacctttgtc acccctgcaa gctcgatgag gggcttttca
     2221 tccacggtta tctcatctcc cggaacgctt ccgcacaata tcgactgctt ttcccaggta
     2281 tcctttccgc caggcaaccg caactgcaat gcatgcagcg atgacgatga ggatctccgt
     2341 gaaaggaatc ttacttatgc cgttcccaaa accgagcatc ccaccgggtc tcctcgtgtt
     2401 ccccgagggt agcccctgcg atgcaccgga tgcctcacct gaagcaggca ctgcggtgct
     2461 tcgaatggat atattcacac ggtcttcgaa cactttccca tccttgtccc tgtactggat
     2521 gatcaaaggt atcgaggata catcgcggac tgtgcaggtg acctcgaaac tggagaagtc
     2581 atccggttcg agagccccta tcacatagac cggattgggg tcgaccggca cagcaggtga
     2641 atccaccgtt actttcactg attttgcatc gcggagaccg gcattcgtga catcaccact
     2701 taacgtgaac gtggaccccg cctgcaccac ctccacgttg ttcactacca tctccgcagc
     2761 tgtcccgccc tctccaacag tcaccggcat ttcgaggacc aggtggtgaa ggttattgcc
     2821 attcctgtag gagacgtcaa aggccattgt cgtatccctc tccgcggtta tttcaaatgt
     2881 ggccttcttc tcctcatcgg gctcaagagt ccc
//
LOCUS       DAOQVJ010000175         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40117_length_2912_cov_4.201960, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000175 DAOQVJ010000000
VERSION     DAOQVJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40117_length_2912_cov_4.201960"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKL26_04625"
     CDS             complement(<1..244)
                     /locus_tag="PKL26_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83013.1"
                     /translation="MDATRPTWGFIVAAIGTLICAWFLDSVPALFLSVFISGFVVFRA
                     LAFLRQAGATASTVSLERGAKPEWLRQGGVAAVSAII"
     gene            complement(248..1216)
                     /locus_tag="PKL26_04630"
     CDS             complement(248..1216)
                     /locus_tag="PKL26_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS83014.1"
                     /translation="MNNLSQDLSLLSGYYREICDRMGDFIVGNEPLIELCMIGLLSGG
                     HLLMEGTPGTGKTSMVKTLARLTGCEFARFQCAVDSQPADILGIRIYDQAKKDFTLRK
                     GPIFTNFLLIDEINRLAPKTQSAFIEAMSERQATLDGITTSIRSPFIVLATQNPFEFE
                     GTFPLIEAQKDRFMFSMTLSHLDSDGELEIIRREHSGRLDWDSYFTRLRPVMNSDVIQ
                     RLLSSVTRLHIEEPLLAYIRDLVMATRSHGDVHIGASSRASIALVRGSKALAALRERS
                     YVIPDDIKELAPRVFMHRIILTREAEISDITSSQVIDEVLERVEVP"
     gene            complement(1213..2103)
                     /locus_tag="PKL26_04635"
     CDS             complement(1213..2103)
                     /locus_tag="PKL26_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNS83015.1"
                     /translation="MPIISRPAWIAAFLVLFGLIAMLSHFSGTTLDYSRYNPEWNGTS
                     RFFEMIGEQDAVTITRFEDLPSHGDALLLMPAPEDEYREDEIAHLVEFLEGGNILVLV
                     SDREDENQITSALGSDIRIQHANLTSIDRYFDHPSSVIAFPAGDDPISAGIPHIVLNR
                     PSYLLGGVPVFESSLLSWVDADGDSRLGSEETLQRYTVIARDNVGEGTLYVIADPSIF
                     INGMLVAGPGDGNARLADNIITARPFLIVDQGHSRTAYAPEMIRVVNLFKESTITKMA
                     LITVIFLAASLLVIPWRRKS"
     gene            complement(2090..>2912)
                     /locus_tag="PKL26_04640"
     CDS             complement(2090..>2912)
                     /locus_tag="PKL26_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83016.1"
                     /translation="EREDTYLYLEPTVPGEGSVALKGTLMAGSTPVRAAPIEILADGK
                     RAGTAYTGEDGKFSIGLKLSPGNRKVKAFFSGDTFPLAPSESAVYEVLVPTPGPGTGN
                     EESFLASPLHAAILAASVCASCVIAFFYLHRRRPGPKAPAPVFHLPGEPVPDPNEAMQ
                     EVLAEGATPAGGDLARPGDERPPGVDAPGDLPSLFSALRLAVSRHLAFLHPLSLTPRE
                     LCRISRDLAVQEAVCRFTNGYEQARYSGAKVPEDELDVIAIAASSAMNSLEGADH"
BASE COUNT          585 a          828 c          905 g          594 t
ORIGIN      
        1 ggatgatggc ggataccgcc gccacccctc cctgcctgag ccattcgggc tttgcccccc
       61 gttcgaggga tactgtcgag gctgtcgccc cggcctgcct gagaaatgcg agtgccctga
      121 acaccacaaa ccctgagatg aagacagaga ggaagagggc aggaaccgag tcaaggaacc
      181 acgcacagat gagcgttccg atggctgcaa cgatgaatcc ccatgtggga cgcgtcgcat
      241 ccacggatca tggcacctcg acgcgttcca gtacctcgtc tatcacctgg gaagaggtga
      301 tatcgctgat ctcggcctcc ctggtgagga taatccggtg catgaagact ctcggggcaa
      361 gctccttgat gtcatcgggt atgacatagg accgttccct gagcgcagcg agcgccttgc
      421 tcccccggac gagggcgatg gatgcccgtg agcttgcccc gatatgcacg tctccatggc
      481 tccgtgtagc catcacgagg tcgcggatgt aggcaaggag gggctcctcg atgtgcaacc
      541 gtgtaacaga agagagtaat ctctggatca cgtcgctgtt catgaccggg cgaagccttg
      601 tgaagtacga gtcccagtca agccggcccg aatgctctct ccggatgatc tcgagttccc
      661 catcgctatc caggtgggag agggtcatcg agaacatgaa acggtccttc tgcgcttcga
      721 tcagggggaa ggtcccctcg aactcgaatg ggttctgggt tgcaaggacg ataaaggggg
      781 accggatgga cgtggtgata ccatcgagtg tcgcctgccg ctcgctcatg gcctcgatga
      841 acgcgctctg ggtcttgggc gccaggcggt tgatctcgtc tatgaggagg aaattagtga
      901 aaatcggacc cttgcggagg gtgaagtcct tcttggcctg gtcgtagatg cgaattccca
      961 ggatatccgc aggctggctg tccaccgcgc actggaacct ggcaaactcg cacccggtca
     1021 gcctggcaag ggtctttacc atcgatgtct ttcccgtgcc gggtgtcccc tccatcagga
     1081 ggtgcccccc cgagagaagc ccgatcatgc agagttcgat gagtggctcg tttcctacga
     1141 tgaagtcccc catcctgtcg catatctccc ggtagtatcc ggacaggagg gagaggtcct
     1201 ggctgagatt gttcaagact tgcgcctcca cgggatgacc aggagcgatg ctgcgaggaa
     1261 gatcacagtg atcagtgcca ttttggtaat ggtagattcc ttgaacagat taaccactcg
     1321 gatcatctcc ggtgcatacg cagtccggga gtgcccctgg tcgactatca ggaacggcct
     1381 tgcggtgatg atgttatcgg caagtcgggc attcccgtca ccagggcctg cgacaagcat
     1441 cccgttgatg aagatgctgg gatcggcgat cacgtagagt gtcccttcgc ccacattgtc
     1501 cctggcaatg accgtgtatc tctggagggt ctcttcgctg cccagccggg agtctccatc
     1561 ggcatccacc caggaaagga gcgagctctc gaagaccggc actcccccca ggaggtaaga
     1621 ggggcggttc agtacgatgt ggggtatccc cgcgcttatc gggtcgtccc cggcagggaa
     1681 ggcgattacc gaagacgggt gatcgaagta ccggtctatg ctggtgaggt ttgcatgctg
     1741 gatgcggata tcactcccaa gtgctgaagt gatctggttt tcgtcctcgc ggtcagacac
     1801 cagcacaagg atatttcccc cttccaggaa ctcgacaagg tgtgcgatct cgtcctcgcg
     1861 gtattcgtct tcgggggcag gcatgaggag gagcgcatca ccatgggagg ggaggtcttc
     1921 aaagcgggtg attgtcacgg catcctgctc cccgatcatc tcaaagaagc gggatgttcc
     1981 gttccactct gggttatacc ggctatagtc cagggtggtc cccgagaagt gggagagcat
     2041 ggcaatcaac ccgaacagga ccaggaaggc cgcgatccac gcaggacggc taatgatcgg
     2101 caccctccag ggaattcatc gcggagctgg cagcaatggc aatcacgtcc agttcgtctt
     2161 ctggcacctt cgcaccgctg tacctggcct gctcgtagcc gttcgtgaac ctgcacacag
     2221 cttcctggac cgcaagatcg cgcgagatcc tgcagagctc ccggggtgtg agggagaggg
     2281 ggtgcaggaa ggccaggtgc cgtgagactg caaggcggag ggcagagaag agggacggga
     2341 ggtccccggg agcatcgaca cccggcggcc tttcgtctcc tggacgtgcc aggtctcctc
     2401 cggcaggtgt ggcaccttca gcgagtacct cctgcatggc ttcattcggg tcaggaactg
     2461 gttcccctgg gaggtggaat actggtgcag gggccttggg tcccggcctt cggcgatgga
     2521 gatagaagaa tgctatcacg cacgaggcac acaccgatgc cgccaggatc gctgcatgaa
     2581 ggggacttgc gaggaagctc tcttcgtttc cggttccagg tccgggtgtg gggacgagta
     2641 cttcgtatac cgcgctctct gagggggcaa gcgggaatgt atccccggag aagaacgcct
     2701 ttaccttgcg gttgccggga gacagcttca acccgatgga aaatttaccg tcttctccgg
     2761 tatacgcggt tcccgcacgc ttaccatctg caaggatctc tatcggtgcg gccctgacag
     2821 gagtcgatcc cgccatgagg gtccccttca acgccacact gccctcccct ggaacggtgg
     2881 gttcgagata aaggtacgtg tcctctctct cg
//
LOCUS       DAOQVJ010000176         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40161_length_2910_cov_4.188792, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000176 DAOQVJ010000000
VERSION     DAOQVJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40161_length_2910_cov_4.188792"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PKL26_04645"
     CDS             complement(<1..1264)
                     /locus_tag="PKL26_04645"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNS83017.1"
                     /translation="MESIKSVILAGGVGTRLWPLSRTYNPKQFLKVNGTSLFQDTWRR
                     ALLISGPEEVWVVTNEAHQFLVRSQLEEVGVHVKDERILKEPVARNTLPAITWAMQVI
                     REEYGDSVVAVFPSDHQLENGAMKEIAAARCISDKYLVTFGVPPTTPHTGYGYIDPGD
                     PLALGARVRKFREKPDWETAKVYLEAGFLWNSGMFLFSTGVFFDELAKYQPELYSQFR
                     DGIREYANLPSLSVDYGLLEHSEKVAVVPLKSRWTDLGTFRAWYELRPHDDYGNAGHA
                     EMLDSERNFVHAPGKRAALIGVRDLVLVDTGDALLACHMDMTEKVSELVKRFRDRGDT
                     ITDYHLQVHRPWGSYTELERSRFFRIKRVTVKPGKRLSLQMHHHRSEHWIIVSGMADV
                     IVGDRTIHLRQGESTFVPAATRHRLANTG"
     gene            complement(1351..1884)
                     /locus_tag="PKL26_04650"
     CDS             complement(1351..1884)
                     /locus_tag="PKL26_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNS83018.1"
                     /translation="MKQAKAGPNILITGAPACGKTTLVEGIAQSLAPIRPEGFVTREV
                     REKGERRGFEMVSFCGKTGILADISIRSGWRVGKYGVDVAAFDAFLEELHFDESPFVI
                     IDEIGKMECLSSLFCMRILDLLDSFPVLLATIALRGTPFIESLKCRDDVEVFCMDSHT
                     RDQVTKHVIQRLRSALD"
     gene            complement(1900..2634)
                     /locus_tag="PKL26_04655"
     CDS             complement(1900..2634)
                     /locus_tag="PKL26_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS83019.1"
                     /translation="MRWRPILGIMALLVTLALQVAPVLADDCGCGGGSGSGDAGDSSD
                     GDSSSGYGSSQDPAAMAATAQDLYMSGHYFEALQAFNDSLAIDPYNAPALMGKAEVLF
                     TLRQYPEAAETYRKVVTISPGHDRAYFCLGNTYLVMGEYQEAAEAYERSLGIRPGNTL
                     AEENLRVARSYMMERVPTEVPTIGISVAPEHETVQATMPPASIPPTDPTTAHSAPMLD
                     GWTGVLSLILSVTIAIAGMKKRTNGA"
BASE COUNT          619 a          851 c          782 g          658 t
ORIGIN      
        1 tgccggtatt ggcaaggcgg tgcctcgtgg cggcaggaac gaacgtgctt tcgccctgcc
       61 ggaggtggat ggtccggtcg ccgacgatca catcggccat tccgctcacg atgatccagt
      121 gctcgctccg gtggtgatgc atctgcaggg agagtctctt gccgggtttt accgtcaccc
      181 gcttgatcct gaagaacctc gaacgttcaa gctcggtata ggagccccag ggccggtgca
      241 cctggagatg gtagtcggtg atcgtatcgc cccggtcgcg gaatctcttc acaagttcgc
      301 tgaccttctc ggtcatatcc atgtggcatg ccaggagcgc atcacccgta tccaccagca
      361 caaggtcgcg aactcctatg agcgctgccc gtttcccggg agcatggaca aagttacgct
      421 ctgaatcgag catctccgcg tggcctgcat tgccgtaatc gtcgtgtggc ctgagttcat
      481 accatgcccg gaacgtccca aggtccgtcc agcgggattt gagggggacc actgccacct
      541 tctccgaatg ctcgaggagc ccgtagtcaa ccgagagcga gggaaggttc gcatactccc
      601 ttataccatc cctgaactgc gaatagagtt cgggctggta ttttgccagt tcgtcgaaga
      661 agaccccggt tgagaagagg aacatcccgc tgttccagag gaacccggcc tccaggtata
      721 cctttgcggt ctcccagtca ggtttctctc tgaactttct tacccgggcc cctaacgcga
      781 ggggatcccc tggatcgata tacccgtacc cggtatgagg cgttgtggga gggactccga
      841 aggtgacgag atacttgtct gatatacatc ttgccgcggc aatctccttc atggcgccat
      901 tttcaagctg gtgatcgctt ggaaagaccg caacgacaga gtctccatat tcttccctga
      961 ttacctgcat ggcccaggtg attgccggaa gggtatttct cgcgacaggc tctttcagga
     1021 ttcgctcatc ttttacatgg accccaacct cctcaagctg gctcctgaca aggaactggt
     1081 gggcttcgtt cgtgacgacc cagacctctt caggccctga tatcaggagc gccctccgcc
     1141 aggtgtcctg aaagagcgag gtgccattca ctttcaggaa ctgcttgggg ttatatgtgc
     1201 gtgacagggg ccagaggcgg gttcctaccc cgcccgcgag gatgacggac ttgatggatt
     1261 ccattcggtt aactctcttt ggacgtgttc gcacttatga tttacccgac cgtatcctct
     1321 aaaaaatatg aaccgactcc ccccactccc tcaatcaaga gcactccgca gacgctgtat
     1381 gacatgcttt gttacctgat cccgggtatg ggaatccatg cagaagacct ccacgtcatc
     1441 ccggcatttc agcgactcga taaagggggt ccccctgagt gcaatggtgg caagcagtac
     1501 cgggaaggag tccaggagat ccaatatacg catgcaaaag agagaagaga ggcattccat
     1561 cttccctatc tcgtcaatga tcacgaatgg tgattcatcg aagtgcaatt cttcaaggaa
     1621 cgcgtcaaac gcagccacat cgaccccata tttccccact ctccaccctg accggatact
     1681 gatatccgcg agaatgccag tctttccgca gaaagagacc atctcgaacc ccctcctctc
     1741 tcccttctcc ctgacctccc tcgtgacgaa gccttcaggc cggattggcg caaggctttg
     1801 ggcaatcccc tccacgaggg ttgtctttcc gcacgccggg gcgccggtga tgagaatatt
     1861 cggcccggcc tttgcctgct tcatggattc acccgccggt tacgcgccat tcgtgcgttt
     1921 tttcatccct gcaattgcga ttgtcacgga taaaatgaga gaaagcacgc cggtccaccc
     1981 gtcaagcatt ggcgcagaat gggccgtggt gggatctgtg ggggggattg acgcaggggg
     2041 catggtggcc tgtacagtct catgctctgg ggcaacagaa attccaatag tcggaacctc
     2101 ggtcggcact ctctccatca tgtacgaccg ggcgacacgg agattctcct ctgcgagcgt
     2161 gtttcccggg cggatgccaa gcgatctctc gtaagcctca gccgcctctt gatactctcc
     2221 catcacgaga taggtatttc caaggcagaa ataggccctg tcgtggcccg ggctgatcgt
     2281 caccactttc cggtaggtct cggctgcttc agggtactgg cgcagtgtga agaggacctc
     2341 cgcttttccc atcagggcag gggcattgta gggatcgatc gcaagtgagt cattgaatgc
     2401 ctggagtgcc tcaaaatagt gtccgctcat atacaggtcc tgcgcagttg ctgccatggc
     2461 agcagggtcc tgcgaagagc cataccctga tgaactgtct ccatcagacg aatcaccggc
     2521 atcgccggaa cccgaacccc cgccgcaacc acagtcatcc gcgagcacgg gagccacttg
     2581 gagagccagg gtgacaagga gagccattat gcccagaatc ggccgccacc tcatgtacta
     2641 tgaccctctg cccacccttg atttaaaggt aatgaaaata gtaaaaaacc gttgattctg
     2701 gtgaccctta gatttactgc gggtatcgat tgtgaaagag gatggatgat gcctccggaa
     2761 gtgtcatgcc ccttgaacct gcttgagtgc cacctcgatt gccactctca gctcctcgtc
     2821 cttgaagggc ttgagtaaaa atccgctcgg accggtctca attgccctcg agagagtctc
     2881 gttatcggaa tgcgcagtca ggtaaatgaa
//
LOCUS       DAOQVJ010000177         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40164_length_2910_cov_3.898774, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000177 DAOQVJ010000000
VERSION     DAOQVJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40164_length_2910_cov_3.898774"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKL26_04660"
     CDS             complement(<1..728)
                     /locus_tag="PKL26_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS83020.1"
                     /translation="MTTTDSIEVIVKEAARDDAGRGIARLSIEAMRALGVVSGDAIEI
                     QGKKKATAIVWPGFSQDTGQAILRIDGTVRGNAGTGVDERVRVRKVEVGYAKKVVINP
                     TQPIRLLQGEQYLSRLLRGRAVMEGQTVRVDVIGNPLTLVITKVSPKGVAIVTEDTQI
                     ELKETPYEPAEKKKGETADVHYEDIGGLSRELEMVREMIELPLRHPELFERLGIEPPK
                     GVLLYGPPGTGKTLIAKAVANEVDA"
     gene            1008..2285
                     /gene="serS"
                     /locus_tag="PKL26_04665"
     CDS             1008..2285
                     /gene="serS"
                     /locus_tag="PKL26_04665"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNS83021.1"
                     /translation="MLDLKFIRENPDAVRQDLARRGDEEKRAWVDDLIEKDRRSRALK
                     LQADELRRRRNSIAREINEERKAGRDTEALRKEASELPGRIREIEAELDEITTTVRFY
                     LMRLPNILHDSVPVGKDDTENVELRRVGSPRTPGFSLASHGQLASDRGWADFERAARA
                     AGAGFYYLKGNLALLDLALQRFAIDLLAERGFVPVIPPYMINRRSYEGVTDLSDFEKV
                     MYKIEDDDAYLIATSEHPIGAMYQDEIFEERDLPLRLAGISPCFRREIGSHGLDTKGL
                     FRVHQFHKVEQFVFCRPEDSWEIHEELLANAEEIFSSLGLPYRVVNICTGDIGTVAAK
                     KYDIEVWMPREEAYREVVSCSNCTSYQAVRANIRVRDPHDFESKRFLHTLNSTAVATT
                     RTIRAILENYQEEGGAVMVPPALRPYMNDLEYL"
     gene            2533..2664
                     /locus_tag="PKL26_04670"
     CDS             2533..2664
                     /locus_tag="PKL26_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83022.1"
                     /translation="MPYHWKKKIDVDETIVVVKSVLTAEKELPNWLINTIYGAIARL"
BASE COUNT          610 a          854 c          864 g          582 t
ORIGIN      
        1 gcgtccacct cgtttgcgac ggcctttgcg atcagggtct tgccggtccc cggggggccg
       61 tagaggagga ctcccttcgg gggctcgatc ccgaggcgct cgaagagctc ggggtgacga
      121 agcggcagct cgatcatctc ccgcaccatc tcgagttccc gcgacagccc gccgatgtcc
      181 tcgtagtgga catccgcggt ctcccccttc ttcttctccg cgggctcgta gggggtctcc
      241 ttgagctcga tctgggtatc ctcggtgacg atcgcgacgc ccttcgggct caccttggtg
      301 atcaccaggg tgaggggatt tccaatcacg tccacccgga cagtctggcc ctccatcacc
      361 gcccggcccc tgagcaggcg ggagaggtac tgttccccct ggaggaggcg gatgggctgg
      421 gtggggttga tcacgacttt cttggcatac cccacctcga ccttgcgcac gcggaccctc
      481 tcgtcgacac ccgtcccggc gttgccacgg acagtcccgt cgatgcggag gatggcctgc
      541 ccggtgtcct gcgagaagcc cggccagacg atggcagtcg ccttcttctt gccctggatc
      601 tcgatggcgt ctccgctcac caccccaagc gccctcatcg cctcgatgct caggcgggcg
      661 atgccccgtc cggcatcgtc cctggccgcc tcttttacaa taacctcgat ggaatcagta
      721 gttgtcatgg gaatatggtt cctgtaatgt ttacttttag taagtgattc atattattta
      781 tacttatttt ataaggaagg ccagatcgat tggacagaaa tccttcctgg accttcaaaa
      841 ggcatggatc cttgaatttt ggtgattttt catgaaagga gccaggtgtc tttttccctt
      901 ccggtggacg tgacggtcga cccgcaaagg caatgcgttc caagcgcaca cctaaactgg
      961 agaggcaaga gcctggatca actgcggcga gaaggtgcaa taaagagatg ctcgacctga
     1021 agttcatacg tgaaaatccc gacgcggtga ggcaggacct cgccaggcgg ggggatgagg
     1081 agaagcgtgc ctgggtggac gacctgatcg agaaggaccg ccgttcccgg gcgctgaagc
     1141 tgcaggccga cgagcttcgc aggcgccgga acagcatcgc cagggagatc aacgaggaga
     1201 ggaaggcagg gcgtgacacg gaggccctca ggaaggaggc atccgagctg ccggggagga
     1261 tcagggagat cgaggcggaa ctggacgaaa tcacgacaac ggtccgcttt tacctgatgc
     1321 gcctcccaaa catcctccac gatagtgtcc ccgtcggaaa agacgacacc gagaacgtcg
     1381 aactccgccg ggtggggtcg ccacgcacgc ccggattctc tctcgcgagc cacgggcagc
     1441 tcgcaagcga ccggggctgg gcggacttcg agcgggcggc ccgggcggca ggtgcgggct
     1501 tctactacct gaagggaaac cttgcgctcc tcgacctcgc gctccagcgg tttgccatcg
     1561 acctgctcgc agagagaggg ttcgtgccgg tcatcccccc ctacatgatc aaccggaggt
     1621 cctacgaggg agtgaccgac ctttctgact tcgagaaggt catgtacaaa atcgaggatg
     1681 acgacgcata cctgatcgca acgagcgagc accctatcgg ggcaatgtac caggacgaga
     1741 tattcgagga gcgcgacctc ccgctccggc ttgcagggat atccccgtgc ttcaggaggg
     1801 agatagggtc gcatggcctc gatacaaagg gcctcttcag ggtgcaccag ttccacaagg
     1861 tggagcagtt cgtcttctgc cggccggagg actcgtggga gatccacgag gaactgctgg
     1921 caaacgccga ggagatcttc tcgagcctcg gccttcctta ccgggtggtc aatatctgca
     1981 ccggcgatat cgggactgtc gccgcaaaga agtacgacat cgaggtctgg atgcccaggg
     2041 aggaggccta ccgggaggtg gtctcctgct cgaactgtac gtcgtaccag gcggtgcgtg
     2101 ccaacatccg ggtccgcgac ccgcacgact tcgagtcgaa gcggtttctg catactctca
     2161 actcgactgc agtagcgacg acgcggacaa tccgggcaat ccttgagaac taccaggaag
     2221 agggaggggc agtcatggtg ccccccgccc tccggcccta catgaacgat ctcgaatacc
     2281 tctgatccgt tcacccggag acccggacaa ccactttttt tagcggcgcc tgggatccat
     2341 gaagatgcgg gtcttttcga agaagggcat ccgccacctg aaaagagaag acaacctcca
     2401 tcattctcga cgggcgggag taaaaacgag aggggggatt cattccgggc tgtccctttg
     2461 gagaaatggc atttgtcggt atccacgtgt ttagagtttg catcgcgtac ttcaccattc
     2521 ggagggccaa aaatgccata ccactggaag aaaaagattg atgtggatga gaccatcgtc
     2581 gtcgtcaaaa gcgtgctgac cgcagaaaaa gaactcccca actggctcat caatacgatt
     2641 tacggggcga ttgcgcgact ctgatcccgc catggtgaaa tacttctatg cagaagtgaa
     2701 aaaatttgtc ccggcaaccc tgaagtattt cgaggaaggt tctacgaggt ctccgattta
     2761 acaaccctct cctttttccg caaataacca gttgcccggg ctgccccggg cctgttccgg
     2821 aaaccgtttc caggtcactc tttgatccag gtgatcccgc attccgtgac gatgatgtcc
     2881 atgcagacat cgttctcgtc cgctggaatg
//
LOCUS       DAOQVJ010000178         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40273_length_2904_cov_3.125307, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000178 DAOQVJ010000000
VERSION     DAOQVJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40273_length_2904_cov_3.125307"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="PKL26_04675"
     CDS             <1..1687
                     /locus_tag="PKL26_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNS83023.1"
                     /translation="FLDHFAAVGLAIPDKTGIASPTAAKWSRNGDYIASVSIPDDPRK
                     YNPNETMWDRWYNWGNPKVTSSWPVMPDGYPKTTSFAPKMPQSVVQTASQFGISPTSS
                     MYNMWLPMAGYTLSFGRDPHYVELMERALDHAPNGGFCNKTDPSGVPYLAVVNQQRAL
                     VGITDSSATGITISPYQWYWNTIQSSRNTTPGHYPYDLGTWWNNVKDLIDDSTSPSEV
                     LNWFWALDTYGTPDYSYFGYSSDAASKKLSQPLCAAFTSSQDQTTFVAWNPHGEPVHV
                     NWWKVGEKAGSAAGVLAQDLIVPPGWYATAGTTPPANASLVVTIDAPVSTRPGSTVTF
                     NVTVRATGPRQVSGVAVSSYLPYSMTYVSSTPAGTVSGNSVRWDAGTLSPGETRSYLV
                     TGKFPDTANGQYTAVAEASGKAAGNIAVKGNRILPISVQGGPSMNVSISADKAAVRRG
                     ENITFTFVNRNDGTQDLEGAYAKLNFQPVGGLSFISANYAPQYTAPDTAVWDPPHLPG
                     TGKLSIGTNITITTTYTVTSSARDPLTTTAYGQANYAGGQANNTVTLTTGVTG"
     gene            1684..>2904
                     /locus_tag="PKL26_04680"
     CDS             1684..>2904
                     /locus_tag="PKL26_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83024.1"
                     /translation="MKKNSMCAGIAALLIVLCLAAVPALAASTGIAWERSLGGSGEEY
                     AKSLQPTNDGGYIAVGDTASTDGDITVSYGMIDVWIAKLDASGEKEWEKTIGGSNDDH
                     VTCIELMSDGTLLLSGYTWSHDGDFSDNHAGIAIWNMQINEEGSILWKGCYPGRSGSF
                     AMDGKETPDGGMIMIGQSFSIPGDPMYHGGYDVLVVKTDQSANTTWERLLGGSGDDFG
                     DNILPLPGGGYIAIGYTNSDDGDVSGNHGFYDAWLFRLDDAGNLLWEKCLGGSLHDEG
                     REIFITPDGNFAVMGYTNSNDGDVSGNHGSFDYWWALVASDGAILSQKCFGGSDYDHA
                     YDLEMTDNGNFVALGVSYSTDGDVSGNAGNGDFWAVEFDTTGAILWERCFGGSGLDRG
                     QTIKPDPSAGGLFFL"
BASE COUNT          635 a          843 c          846 g          580 t
ORIGIN      
        1 gttcctcgac cacttcgcgg cggtgggact cgcgatccct gacaagacag ggatcgcgag
       61 tcccaccgcc gcgaagtggt cgaggaacgg agattatatc gcatccgtga gcattcctga
      121 tgaccccagg aaatacaatc caaatgagac gatgtgggac cgctggtaca actgggggaa
      181 cccgaaagtc acgtcctcct ggccggtgat gccggacggg tacccgaaga ccacctcgtt
      241 tgccccgaag atgccccagt cagtggtcca gaccgcctcg cagtttggga tctcccccac
      301 ctcgtccatg tacaacatgt ggctcccgat ggcaggatac acgctctcgt tcgggaggga
      361 tccgcattac gtggagctca tggaacgtgc gcttgaccat gccccgaacg gcgggttctg
      421 caacaagacc gatccatcag gtgtgccata cctcgcggtg gtcaaccagc agcgtgccct
      481 tgtaggaatc acggacagtt cggcgacggg aatcaccatc tctccatacc agtggtactg
      541 gaacaccatc cagtcctcgc ggaacaccac cccgggccac tacccctacg acctcgggac
      601 atggtggaat aacgtaaaag acctgatcga tgattccacg agcccatctg aagtcctgaa
      661 ctggttctgg gctctcgaca cgtacggtac ccctgattac tcgtatttcg gatactcttc
      721 cgacgccgcc tcgaagaaac tctcacagcc gctctgtgcc gcctttactt caagtcagga
      781 ccagacaact tttgtcgcgt ggaacccgca tggcgaaccc gtgcacgtga actggtggaa
      841 ggtaggggag aaggctggtt ccgctgctgg tgttcttgct caggatctca ttgtgccacc
      901 gggatggtat gcgacggcag gcaccacccc tcccgcgaac gcatcgctcg ttgtgactat
      961 cgatgctcct gtaagtaccc gccccgggag cacggtcacc ttcaacgtga ccgtcagggc
     1021 gaccggccca aggcaggttt ccggtgtcgc ggtctccagc tatctcccgt actctatgac
     1081 atatgtctcg agcactcctg ccgggacggt ctccggcaat tccgtcagat gggatgcagg
     1141 cacgctctct cccggggaga ccaggtcata cctcgtgacc ggtaagttcc cggacaccgc
     1201 caacgggcaa tataccgcgg tcgccgaagc atccgggaag gctgccggaa atatcgcggt
     1261 gaaaggcaac cggatcctcc ctatctcagt ccagggcggt ccctcgatga acgtctccat
     1321 ctctgcagat aaggctgcgg tcaggcgtgg ggagaatatc acgttcacct ttgtcaacag
     1381 gaacgatgga acacaggacc ttgagggagc gtatgcgaaa ctgaatttcc agccggtcgg
     1441 tggtctctct ttcatctccg cgaattacgc accccagtac actgccccgg atactgccgt
     1501 atgggacccg ccacatctcc cgggcaccgg caaactgtca atcgggacca acatcacgat
     1561 cacgacgacc tatacggtca catccagtgc cagggatcct ttaaccacga ccgcctacgg
     1621 gcaggcgaat tatgccggcg gacaagcgaa caataccgtg actctcacca cgggggtgac
     1681 gggatgaaga agaattccat gtgtgcaggg attgccgcac ttctcatcgt gctctgcctg
     1741 gctgcagtcc cagcgcttgc agccagtacc gggatagcat gggagagatc tctcggcgga
     1801 tccggggaag agtatgcaaa atctctacag cccacgaacg acgggggcta cattgccgtg
     1861 ggggacaccg cctcgacaga cggggacatc acggtctcat acgggatgat cgatgtctgg
     1921 attgcaaaac tcgatgcgtc cggggagaag gaatgggaaa agaccatcgg gggatcgaat
     1981 gacgatcacg tgacctgtat cgagctgatg agtgacggca ccctgttgct ctcgggctat
     2041 acctggtcgc atgacggtga tttctctgac aaccacgccg gaatcgcgat atggaacatg
     2101 cagatcaacg aggagggatc cattctctgg aaagggtgtt accctggtcg aagcggcagt
     2161 tttgccatgg atggcaaaga aacccctgac ggagggatga tcatgatcgg ccagtcattc
     2221 tctatccccg gagacccgat gtaccatggc ggctacgatg tcctggtagt gaagacggac
     2281 caatctgcaa acaccacctg ggagaggtta ctcggcgggt cgggggatga cttcggggac
     2341 aacatcctcc cgctcccggg cggagggtat atcgccatcg ggtacacgaa ctcggacgac
     2401 ggcgatgtct ccgggaacca cggcttctac gacgcctggt tgttcaggct tgatgatgcc
     2461 ggaaacctgc tctgggagaa atgcctcggc gggagcctcc acgatgaagg ccgggagatc
     2521 tttatcactc ctgacggaaa ttttgcggtg atgggatata ccaactcgaa cgacggggat
     2581 gtttccggga accacggttc attcgattac tggtgggcgc tggttgcctc tgacggagcg
     2641 atcctttcac agaaatgttt cggagggtcg gactatgatc acgcctatga cctcgaaatg
     2701 accgataatg gaaactttgt ggcactcggc gtctcttatt cgaccgatgg agatgtcagt
     2761 ggaaatgccg ggaacggtga cttctgggca gtcgagttcg atacgaccgg ggcgatcctc
     2821 tgggagcggt gcttcggagg gtcaggactt gaccggggcc agaccatcaa gcccgatcct
     2881 tctgcaggag gcctcttctt cctg
//
LOCUS       DAOQVJ010000179         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40356_length_2900_cov_4.575747, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000179 DAOQVJ010000000
VERSION     DAOQVJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40356_length_2900_cov_4.575747"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKL26_04685"
     CDS             complement(<1..713)
                     /locus_tag="PKL26_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS83025.1"
                     /translation="MNPLITVENLCMDFDGKRVLHNISFEIGEGEVLGIIGRSGAGKT
                     VLMHLMRGVEQPPTEGKIIYHVAVCDSCPFLGVGSLAGKPCPGCGKTLKARDIDLWNE
                     PDEGMRRRLMRHTAIMFQRTFALYGNDRVIENVLHALDDIGYPQEKAINRAADLLDQV
                     RLSHRMMHIARDLSGGEKQRVVLARQLAKEPFLLFADEPTGTLDPETAHLVHSMLIEA
                     AETNHMGMVVTSHFSQVIN"
     gene            complement(787..1599)
                     /locus_tag="PKL26_04690"
     CDS             complement(787..1599)
                     /locus_tag="PKL26_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS83026.1"
                     /translation="MSIAVVFDSAGTLLRTYRVAKDVLKGELLPGIETVSLTFSSPER
                     VLVVIHVHSREVIETPPEVPLSRYLVEHQVGFGVSCARKVIVADEVAAALYQSPHGTI
                     GDLQDCIRNVWHVCKRESVVAMNSGVIVNMGLSGIEFTVTTGGRPFEGARESISELHR
                     MGIPTYIASGDRVTKLEKMADFLGIPRDRVFGNATPTVKAQIVADLQEEYDRVLMVGD
                     GINDICAMKKSDVAILTEEQSGERHPELYQAADHVVQNVREVVAIVKRLESR"
     gene            1663..2604
                     /locus_tag="PKL26_04695"
     CDS             1663..2604
                     /locus_tag="PKL26_04695"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HNS83027.1"
                     /translation="MLADTAEKIKRMEIRGAGRIARAAVEALDEYSGALPEGSLQGYL
                     ERMDEAAALLIATRPTAVSLPNGVRSVMSVLRQQFSSVEEAKQAFHERCRAFIVASGQ
                     AVQRIGEIGSRHIRDGDVLLTHCNSEAALACILEAKRQGKEISVFATEVRPRNQGHLT
                     IRTLDEAGIPTSFIVDSAARYFMNEVDLVVVGADAVSVNGAVVNKIGTSQIALAAREA
                     RTRVMVAAETYKFAPRTILGEYIEIEERDPGEVLSPALSGQLPNVRVRNPAFDVTPAD
                     YVDLIVTEAGAIPPQMAYIIIRDVLGWDIGEYSGGFG"
     gene            2740..>2900
                     /locus_tag="PKL26_04700"
     CDS             2740..>2900
                     /locus_tag="PKL26_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNS83028.1"
                     /translation="MELHFTKLQGNGNDFVLVDEYEGVAIPDDMKAQFAALYCDRRFG
                     IGADGVLYLS"
BASE COUNT          558 a          839 c          903 g          600 t
ORIGIN      
        1 tcgttgatca cctgggagaa gtgggaggtg acgaccatgc ccatgtggtt cgtctcggcc
       61 gcctcgatca gcatggaatg gacaaggtgg gcggtttcgg ggtcgagcgt gccggtcggt
      121 tcgtccgcaa agaggaggaa gggctctttt gcaagctggc gggcgagcac cacccgctgt
      181 ttctcccctc ccgacaggtc gcgggcgatg tgcatcatgc ggtgggagag gcgcacctgg
      241 tcgaggaggt ccgcagccct gttgatggct ttctcctgcg ggtaaccgat gtcgtccagg
      301 gcgtgaagga cgttctctat cacgcggtcg tttccataca gggcaaatgt cctctggaac
      361 atgatggcgg tatgccgcat gagcctccgc ctcatgccct cgtcgggctc gttccagagg
      421 tcgatgtccc tggccttgag cgtttttccg cacccggggc atggctttcc ggcgagactg
      481 cctacgccaa gaaaggggca gctatcgcag accgcgacgt gatagatgat cttcccctct
      541 gttggaggct gctccacacc cctcatcagg tgcatgagca cggtctttcc ggccccactg
      601 cggccgatga tcccgaggac ctccccctca ccgatctcaa aagaaatgtt atgaaggacc
      661 ctttttccat cgaagtccat acagagattc tcaacggtga tcagcgggtt cattctcttc
      721 ctcttactag acaatatgat gcatggtgca tattactttt catcttgcat cggatcctgg
      781 ccattctcat cgactttcga gtctttttac gatggcgacg acttcccgca cattctggac
      841 cacgtggtcg gcagcctggt agagctcggg gtgcctctcc cctgactgct cctctgtgag
      901 aatggcgaca tccgacttct tcatggcaca aatgtcgttg atcccgtcac cgaccatcag
      961 cacccggtcg tactcctcct ggaggtctgc gacaatctgg gccttgaccg tgggcgtggc
     1021 attcccgaat acacggtccc gggggatgcc gaggaagtct gccatcttct cgagttttgt
     1081 cacccggtcg ccggaagcga tgtaggtggg gatccccatg cggtggagct cgctgatgga
     1141 ttccctcgca ccctcgaacg gacggccgcc ggtggtgacg gtgaactcga tgccggaaag
     1201 acccatgttt acgatcaccc cgctgttcat ggcaaccacc gattcccttt tgcagacatg
     1261 ccagacgttc cggatgcagt cctggagatc gccgatagtg ccatggggtg actggtagag
     1321 tgcagccgcg acctcgtcgg cgacaatgac tttccgggcg cagctcaccc cgaaccccac
     1381 ctggtgctcg acgagatagc gggagagggg gacttccggc ggtgtctcga tcacctccct
     1441 tgagtggacg tggatcacga cgagtacccg ctctggggag ctgaacgtga gcgagactgt
     1501 ctcgatgccg ggaaggagtt ctcctttgag cacgtccttt gcaacccggt aggtacggag
     1561 cagggtcccg gcgctgtcaa agaccacggc gatggacatc ggaaaagcgt ctcccgtata
     1621 ttttatgtgc gcataaccgg cactaggtac atagtgcccg caatgctagc tgataccgca
     1681 gagaagataa agcgcatgga gatccggggg gcgggtcgga ttgcaagggc ggccgtcgaa
     1741 gctctcgatg aatattccgg ggccctccca gaagggagcc tgcagggata cctggagagg
     1801 atggacgagg cagcggcact cctcattgcc acccgcccca ctgccgtctc cctcccaaat
     1861 ggcgtccgat cggtgatgtc ggtcctgcgg cagcaatttt cgagcgttga ggaggcaaaa
     1921 caggcattcc atgagcgttg cagggcgttt atcgtcgcgt caggtcaggc ggtccagcgg
     1981 atcggcgaga tagggtcccg gcatatccgc gacggtgatg tcctgctcac ccactgcaac
     2041 tcggaagcgg cccttgcctg catccttgag gcgaagcggc agggaaagga gatctcggtc
     2101 tttgcaaccg aggtccgccc ccggaaccag ggccacctga ccatccggac ccttgatgag
     2161 gcagggatcc cgaccagttt catcgtcgat tctgcagccc ggtacttcat gaacgaggtg
     2221 gacctggtgg tggtgggtgc cgacgccgtc tcggtgaacg gcgcggtggt gaacaagatc
     2281 gggacctcgc agatcgccct tgccgcccgc gaggcgagga cacgggtaat ggtcgccgcg
     2341 gagacctaca agtttgcacc gcggactatt ctcggggagt acatcgagat cgaggagcgt
     2401 gacccgggag aagtcctctc ccccgcgctc tcaggacagc tcccgaatgt ccgggtgaga
     2461 aacccggcgt ttgacgtgac tcctgccgat tatgtcgatc tcatcgtgac agaggcaggt
     2521 gccatcccgc cgcagatggc ctacatcatc atccgggatg tgcttgggtg ggatatcggg
     2581 gagtattcgg ggggatttgg gtaaccgcat gaccgtacac cgggggtggt gcggggaccc
     2641 atttgccggg cgaatgattg aaagagtgca tgaccataca ctatacagac ccgcgtgatc
     2701 agttccgggg cgacacgggt aatggaggat gtcaggggca tggagttaca ctttaccaaa
     2761 ctgcagggga acggaaacga cttcgtcctc gtcgatgagt acgagggggt ggcaataccc
     2821 gacgatatga aggcacagtt cgctgcactg tactgcgacc gcaggttcgg catcggcgcc
     2881 gacggggtcc tctacctctc
//
LOCUS       DAOQVJ010000180         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40364_length_2900_cov_3.557469, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000180 DAOQVJ010000000
VERSION     DAOQVJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40364_length_2900_cov_3.557469"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKL26_04705"
     CDS             complement(<1..885)
                     /locus_tag="PKL26_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HNS83029.1"
                     /translation="MKRSNEGHYNYKAILALAVLLILVAPFSAAENTIPQGGTVFVGE
                     EDLDITLTGATAGSSLAWYGTGGQVSHAPAAQVTVGDPSAFYVSPITFGGKTGPWFLL
                     PENTVAFYLEEPSLGIRVVDYSSGFVVSPSATWIPKGDTAGFRVDTNLWVMANRPGTA
                     GAPLSIRLNGPDSLTFSSLGGYSLEGVVVSSSPFETGPVWATGSAEYPIGEYSVYVRC
                     DANDMSDNYPAAGKAISEKVTFLVQRVNPLITGTTPLPVTSVPTATRTPTPAGSPPAS
                     PIETATTATPTVQETGTPL"
     gene            complement(959..2161)
                     /locus_tag="PKL26_04710"
     CDS             complement(959..2161)
                     /locus_tag="PKL26_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNS83030.1"
                     /translation="MTGELRIVGTAHVSAKSVEEVRAAIEEFSPDVVAVELDPARYAV
                     IRKQAKDPSVQDILEAKNFTQILVQWILAYLQRRIGLDLGVEPGAEMKAAIEEAESRG
                     IAIGLVDRDINITLRRFWRSLSFFEKLKMLYALAVSVASIDGEEIDVEQLKEQDMLTA
                     AMEEFRKFSPNGARAIIDERDAYLAHNLSGLTTRHARVLAILGAGHVEGVRKYLDAPG
                     TLPPMQQLTAEVKSRPWGLIFGVAVTVLFVLLLLALAFSGVGFEVLGMALVYWVLVHG
                     ILTAAFTLAARGHPLSALTGFGVSWFTALNPLIAAGWFSAIVEAKIRKPAPADFRRIF
                     EAESFSEMMKVPLFRVVLVAALANLGSTLGTVLYFMFIFPVLGIDPGVLITQGFSNMW
                     NAITGIFS"
     gene            complement(2158..2709)
                     /locus_tag="PKL26_04715"
     CDS             complement(2158..2709)
                     /locus_tag="PKL26_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNS83031.1"
                     /translation="MKENESSMCEETLAARLQEIGFSCTRCGECCRAGNGDDNLVMVS
                     PAEVRRISAHCRLDAAEVAEPYPESVEISGGRRITFGWAIRRISGDCMFYEGGSCRAY
                     ATRPWICRTYPFMLDGDTLLTFPCPGLGSPVGPEEARALARDLLARRAAEQEQEERIR
                     RAFSMLPQGEGRLIVDGEGVKGV"
     gene            complement(2706..>2900)
                     /locus_tag="PKL26_04720"
     CDS             complement(2706..>2900)
                     /locus_tag="PKL26_04720"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314287.1"
                     /note="type-1 glutamine amidotransferase domain; functions
                     to produce GMP from XMP in the IMP pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HNS83032.1"
                     /translation="HADEVAEAPPGFRLLARSAICPHEAIARPDLHLYGLQWHPEVSH
                     TCDGLRVYENFDAICREQGA"
BASE COUNT          571 a          926 c          877 g          526 t
ORIGIN      
        1 caggggcgtt ccggtctcct ggaccgttgg cgttgcggta gtagcggtct caatcgggct
       61 cgccggagga gacccggcag gcgtgggagt gcgggtcgcg gtcggcacac tcgtcaccgg
      121 cagtggagtc gtccctgtaa tcagcgggtt gactctctgg acgaggaatg tgaccttctc
      181 gctgatggcc tttcccgcgg ccgggtaatt gtcggacatg tcgtttgcat cgcaccgtac
      241 ataaacagaa tactccccga tgggatactc ggcactcccg gtcgcccaca ccgggccggt
      301 ctcaaacggc gacgaggaga cgaccacacc ctcgagagaa tacccgccga gggatgagaa
      361 tgtcagcgag tccgggccgt tcagcctgat ggacagcggc gcgccggcag ttcccggccg
      421 gttcgccatg acccagaggt tcgtatcgac ccggaatcct gcggtgtccc ccttgggaat
      481 ccaggtcgcc gacgggctga ccacgaaccc ggaagagtag tccaccaccc gtatcccgag
      541 gctcggctct tccaggtaga atgcgacagt attctcgggg aggaggaacc acgggccggt
      601 cttccctcca aacgtaattg gagagacata gaacgcggac gggtcaccga ccgtcacctg
      661 ggccgcgggg gcgtgcgaga cctgcccgcc agtaccatac caggcaagcg aggaccccgc
      721 ggtggcaccg gtcagggtga tgtcaaggtc ctcctctccg acaaagaccg tccccccctg
      781 gggtatggta ttctctgccg cggagaaggg ggcgaccagg ataaggagga ctgccagggc
      841 aaggatcgct ttgtaattat agtgaccctc attcgatcgt ttcatagaca ggagagcgca
      901 aacccgcctc ataaaccttc tcctgcgatg aagaaggagg ttttttaagg acttcctttc
      961 aggagaagat cccggtgatg gcattccaca tgttggagaa cccctgggtg atcagcaccc
     1021 cgggatcgat tcccagcacc gggaatatga acataaagta gaggacagtc ccgagcgtgc
     1081 tccccaggtt ggcaagcgcg gcaacgagga cgacccggaa gagcggcact ttcatcatct
     1141 ccgagaaact ctcggcctcg aagatccggc ggaagtcagc aggtgccggt tttcgtatct
     1201 tcgcctccac gatggccgag aaccagcccg ctgcaatcag tgggttcagc gccgtgaacc
     1261 aggagacccc aaatcccgtg agtgcggaga gggggtggcc cctggcggca agggtaaatg
     1321 ccgcggtcag gatgccgtga accagcaccc agtacacgag tgccatcccc agcacctcga
     1381 agcccacgcc cgagaacgcg agcgccagga ggaggaggac aaagaggacc gtcaccgcca
     1441 ctccgaagat caggccccag ggccgcgact tcacctccgc agtcagctgc tgcattggcg
     1501 ggagagtccc gggtgcatcg aggtacttcc ggaccccctc gacatgccct gccccgagga
     1561 tggcaaggac ccgtgcatgc cgcgttgtga ggcctgagag attgtgggca aggtaggcat
     1621 ccctctcgtc gatgattgcg cgtgcaccgt tcggggagaa cttccggaac tcctccatgg
     1681 cggcagtgag catgtcctgc tccttcagct gctcgacgtc tatctcctcg ccatcgatgc
     1741 tggcgaccga gaccgcgagg gcatagagca tcttcagctt ctcgaagaac gagagcgacc
     1801 tccagaacct gcgaagggtg atgttgatgt cccggtcaac gaggccgatg gcgatccccc
     1861 gcgactctgc ctcctcgatg gctgccttca tctcggcacc cggctccacg cccaggtcaa
     1921 gcccgatgcg tcgctggagg tatgcgagga tccactggac gaggatctgg gtgaagttct
     1981 ttgcttccag gatatcctgg accgacgggt cttttgcctg ttttcgtatc accgcatacc
     2041 gggcggggtc aagttccact gccaccacgt cgggagagaa ctcctcgatg gctgcccgga
     2101 cctcctccac gctctttgcc gagacatggg ctgtgcccac gatccggagt tcccctgtca
     2161 tactcccttc actccttcac catcaacgat aaggcgcccc tccccttgcg gaagcatcga
     2221 gaacgcccgc cgtatcctct cctcctgttc ctgttctgca gctctccgcg caagcaggtc
     2281 gcgggccagg gcgcgggcct cttccggacc aacagggctc ccaagcccgg ggcagggaaa
     2341 cgtgagcaac gtatcgccgt caagcatgaa gggataggtg cggcatatcc agggcctcgt
     2401 ggcataggcc cggcacgatc ccccttcgta gaacatgcag tcgccgctga tacgccgtat
     2461 cgcccatccg aacgtgatcc tgcggccgcc cgaaatctcc acggactcgg ggtagggctc
     2521 ggcgacctct gcggcatcga ggcggcagtg tgcggatatc cggcggacct ctgcaggaga
     2581 taccatcacc aggttgtcgt ccccgttccc cgcgcggcag cactcgccgc acctggtgca
     2641 ggagaaccct atctcctgga ggcgggcagc cagggtctcc tcgcacatgg atgattcgtt
     2701 ctccttcatg ctccctgttc cctgcagatt gcgtcaaaat tctcatagac cctgagacca
     2761 tcgcaagtat ggctcacttc agggtgccac tggaggccat acaagtgcag atctggcctt
     2821 gcgatcgcct catgcgggca gatggcagac ctcgcaagca gccggaaacc aggaggtgcc
     2881 tccgcaacct cgtccgcgtg
//
LOCUS       DAOQVJ010000181         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40544_length_2891_cov_3.893512, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000181 DAOQVJ010000000
VERSION     DAOQVJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40544_length_2891_cov_3.893512"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..482
                     /locus_tag="PKL26_04725"
     CDS             111..482
                     /locus_tag="PKL26_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNS83033.1"
                     /translation="MPEFATAFSGMNSDRKLSHSELVRAIRLMIADEYEAIQVYTQLA
                     ESIDNTLAKEVLLDIADEERVHAGEFLRLLQELDPDEMKFYAEGAAEVEAEIAKISPG
                     GTSPATAEKKGGATTIGSMKE"
     gene            493..1290
                     /locus_tag="PKL26_04730"
     CDS             493..1290
                     /locus_tag="PKL26_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNS83034.1"
                     /translation="MTNPYLGREDAPIQPETWSLLDSAMVEAAKSVLAGRRLLHIEGP
                     YGLGLKAVPLEDCQVSECMAVSQFVPVHLISTTFTVGIRDLLAFEQNGLPFSAEAAAE
                     AAIECARNEDALIFEGGMGSPGLIEMEGTGALKLSSWSTVGKAADDIIQAMIRLDEAG
                     YHGPYSLALAPSRYNLLFRRYLQGQSTELEHIRAIVTDGVYKAPALKSGGVLLASGRR
                     YASIVLGQDMTVGFIGPIGDSLEFSITESLALLVREPSSICVLKDKE"
     gene            complement(1313..>2891)
                     /locus_tag="PKL26_04735"
     CDS             complement(1313..>2891)
                     /locus_tag="PKL26_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNS83035.1"
                     /translation="TIANPGEFASRLCGLPFREIAQDGSPHGKRTFLFYNPYHSWPAL
                     RSVHWDTALIFSRCMDHGMQTLCFTGARKSAELVAFRASEMNPGKGGIAAYRAGYLPE
                     ERRKIEQGLKSGTLRGVVSTNALEVGIDIGSLGGVILSGFPGTMMAAWQQAGRAGRSL
                     GESLAALVAFANPLDQYFMRHPQEFFGAAHEHAIVDLGNPYILSGQVLCAGAELPIDC
                     ERDQEFFGPSLPDIVAAHARESLLGKTRRGWIYSGSRRASDLVTMDGSSRETFRVLSQ
                     GRVIETMDRSQAFREAHPGAILLHQGEQYRVDSCDLAAKTVHATPVDVDYYTRPLKSV
                     NIRVTGIRERRSLPGAPLTFGDVEVTEQFTACKLLRSETVIGIEPLDLPPLQFSTRAL
                     WVEVPPEAVESVGQQGGDLPGGLHAMEHAMIAMMPFHVLCDRWDIGGLSVPCGGDLGD
                     PTIYVYDGFEGGIGLAEKAYGICEEILGTAHTLVRDCRCESGCPACILSPKCGNDNQP
                     LDKAAALQLLSLVSGGK"
BASE COUNT          562 a          896 c          912 g          521 t
ORIGIN      
        1 tcccttccgg cacggttccg gagagggaac catattaacg tggaaggtga aataaggcta
       61 acgaggggtc tcgttcaatt gggcccttgt tacatgagga ttgatgaggt atgcctgaat
      121 ttgcgactgc attttctgga atgaacagcg accggaagct ctcccacagc gagcttgtca
      181 gggcgatccg gctgatgatc gccgacgagt atgaagccat ccaggtctac acccagcttg
      241 cagagtcaat tgacaacacc cttgcgaagg aggtactcct ggatattgcg gacgaagagc
      301 gcgttcacgc aggggagttc ctgcggctgc tccaggagct ggaccctgac gaaatgaagt
      361 tctacgcgga aggcgccgcc gaggtcgagg ccgagattgc aaagatctcc ccaggtggca
      421 catccccggc aacggcagag aagaagggtg gggcgaccac catcgggtcc atgaaggagt
      481 gagaggacga tcatgacaaa cccttacctt gggagggagg atgctcccat acagcctgag
      541 acctggtccc tgctcgattc ggccatggtc gaggctgcaa agagcgtcct tgccggccgg
      601 aggctcctcc acatcgaggg gccctacggg cttggtctca aggcagtccc gctcgaggac
      661 tgccaggtga gcgagtgcat ggcggtcagc cagttcgtgc cggtgcacct gatcagcacg
      721 acgttcactg tgggaatcag ggacctgctt gcatttgagc agaacggcct tcccttctct
      781 gcagaggcag cagccgaggc ggccattgag tgcgcacgaa acgaggacgc cctcatattc
      841 gagggcggca tggggagccc cggcctcatc gagatggagg ggaccggcgc actcaagctc
      901 tcgtcgtgga gcacggtggg aaaagctgcc gatgacatca tccaggcaat gatccgcctc
      961 gacgaggcag ggtatcacgg cccgtacagc ctggcgctcg cgccctcacg gtacaacctc
     1021 ctcttccgcc gctacctgca ggggcagagc acggaactcg aacacatccg ggcaatcgtc
     1081 acagacggcg tgtacaaggc ccctgccctc aagagcggag gagtcctgct ggcctcgggg
     1141 aggcgctacg cctctatcgt gctcggccag gacatgaccg tcgggttcat cggcccgatt
     1201 ggggacagcc tggagttctc catcacagag agcctcgcac tcctcgtgag agagccgtcc
     1261 tcaatctgcg tcctgaagga caaggaataa tcttcatccc cctttttccc tttcatttac
     1321 ccccggatac gagcgagagg agctggagcg ctgcggcctt gtcgaggggc tggttgtcat
     1381 tcccgcattt cggcgagagg atacaggcag gacaccctga ttcacaccgg caatccctga
     1441 cgagggtgtg tgcggtcccc agtatctcct cgcatatccc gtaggctttc tcggcaaggc
     1501 ctatccctcc ctcgaacccg tcgtagacgt agatcgtcgg atcgccgagg tcacccccgc
     1561 aggggaccga gagacctccg atgtcccacc ggtcgcagag gacatggaag ggcatcatcg
     1621 cgatcatggc atgctccatg gcgtggagac cgcccgggag gtctccaccc tgctgcccga
     1681 ctgattccac cgcttccggg ggcacttcga cccagagtgc gcgggtggag aactgcagcg
     1741 gggggagatc gagcggctcg atccctatga cagtctcgga cctgagaagc ttgcaggcag
     1801 tgaactgctc tgtcacctcc acgtcgccga aggtgagcgg agccccgggc agcgatcgcc
     1861 tctcccggat acccgtgaca cggatattca ccgatttcaa cgggcgggta tagtaatcga
     1921 catccacggg ggtcgcatgg acggtctttg ccgcaaggtc acacgagtcc acccggtact
     1981 gctccccctg gtggaggagg atcgcgccag ggtgggcctc gcggaacgcc tgggaccggt
     2041 ccattgtctc gatcacccgg ccctgcgaga gcaccctgaa ggtctcgcgg gaactgccat
     2101 ccatcgtcac gaggtctgat gcgcgccggc tcccggagta gatccacccc ctccgggtct
     2161 ttccgagcag cgactccctg gcatgggctg cgacgatatc cgggagggac ggaccgaaga
     2221 actcctggtc ccgctcgcag tctatgggga gctcggcccc ggcgcagagg acctgcccgg
     2281 agaggatgta ggggttcccg aggtcgacga ttgcgtgctc gtgggcggcg ccgaagaact
     2341 cctgcgggtg ccgcatgaag tactggtcga gggggtttgc gaaggccacc agcgcggcaa
     2401 gcgactcccc gagggagcgg cccgcccgcc ctgcctgctg ccaggcggcc atcatcgtcc
     2461 cagggaaccc tgagaggatc acccccccga gcgaaccgat gtcgattccc acctcgagtg
     2521 catttgtgga aaccacaccc cgaagggtcc cgcttttgag tccttgctcg atctttctcc
     2581 tctcttccgg aaggtacccg gcccggtagg ccgcgatgcc gcccttcccg ggattcatct
     2641 cactcgcccg gaacgccacg agttccgcag attttctcgc tcccgtgaag cagagagtct
     2701 gcataccgtg gtccatgcac cgtgagaaga tgagtgcagt gtcccagtgg acagagcgca
     2761 gcgccggcca tgagtggtac gggttgtaga agagaaatgt ccgtttcccg tggggggagc
     2821 cgtcctgcgc gatctccctg aagggaagcc cgcagagcct ggaggcgaac tcccccgggt
     2881 ttgcaatcgt g
//
LOCUS       DAOQVJ010000182         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_40795_length_2878_cov_4.014878, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000182 DAOQVJ010000000
VERSION     DAOQVJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40795_length_2878_cov_4.014878"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..960
                     /locus_tag="PKL26_04740"
     CDS             394..960
                     /locus_tag="PKL26_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HNS83036.1"
                     /translation="MTQYGQEDVNKYRKFKKVDGATYRKVNQFLRKRTYVTAREWAIA
                     RLCTDFRTRSGAEMTFIGQHLPELVPFMQECYTPQAVNQARNSFKKKVYKSGATFFYG
                     AMCGFFTSEELDDLLFEASEVARFLLEVERTSLDIDEEIEIEDRITDVMRSVGEAAAQ
                     ILRARMHNEDQTGEEPEVAGNPPDQEQV"
     gene            957..1715
                     /locus_tag="PKL26_04745"
     CDS             957..1715
                     /locus_tag="PKL26_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNS83037.1"
                     /translation="MRVIHIAGVSGSGKTTFIRSLIPLLEQKGPTAVAKHLGHHCYSL
                     EKGKDTTIFMEEGAHVSAGVDIEKTVVVARGLSFGDILPLLSSIGTEYLLVEGWKTQP
                     LPKVAIGDLPGAAEVILSNPTAAEVIASLDLFPRYYSLEGLAQKVQEGCTGGGVVLTG
                     RYPVPDHAGNPESRREFYLRFSPTLHEISRVAESRPGGVRTMVHLHQGLLFGGEDGIL
                     VAVGAPTPADALDAFSSCHRHLLSALGTGSPHQG"
     gene            1718..>2878
                     /gene="glmM"
                     /locus_tag="PKL26_04750"
     CDS             1718..>2878
                     /gene="glmM"
                     /locus_tag="PKL26_04750"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS83038.1"
                     /translation="MEGQKLEKKLFGTNGVRGVAGREMTPALALSIGAALGTMRQGTI
                     AVGRDTRTSGPALACALKAGLLSAGCDVVDLGVLPTPALQYLVRDHFDAGSVVTASHN
                     PPEYNGIKVVEADGTEMGDEQSILLEDHIFRGTAYQAAWDHVGREHAAPYMVEDYVRA
                     VAGSFPTGTGGGTLVAADPGCGAAAWTTPEILTRMGCRVFSINSQMDGTFPGRLPEPS
                     PEGLAPLADLVKATGARFGVAHDGDADRAVFIDDQGRFIEENRQFGLIADFICRNRRG
                     VVVTPVSTSRLIEVIAHRHGCSVVYTAVGSIYVARTMIGLSKQGTAVAFGGEGNGGLI
                     FPAHQLCRDGGMTAATMVALLADTSQPLSTLVDALPPFFMIKEKLQKKDHIVI"
BASE COUNT          656 a          808 c          886 g          528 t
ORIGIN      
        1 gttgctgtag atacctcccg agtcctgaat gacacgtcaa tcatcgggcg gctcatccac
       61 gcccgttctt tggtgaggcc cggcaaccac ctggaacctg gacaatcgta gtggaacgtt
      121 gagacaggca aaaaaatggt tggggtgttg aaaagaagga cccatgcatg tgtgcattta
      181 cacttcacca aatgatttac acggcctgct gatcttttcc tgaaataatt cattatccac
      241 ctttttttta aaaaaattgc gtgatattgc actttaaata aaaacatccg cagcactata
      301 ataaactata taatgcgata tgataagaaa ttgaaagagt atacgggcgc aggaagcgat
      361 cggggctcaa atgcgcaagg gaggacccaa atcgtgacgc aatatggaca ggaggatgtg
      421 aacaagtacc ggaaattcaa gaaggtggat ggtgcgacct accggaaggt gaaccagttc
      481 ctgagaaagc ggacctacgt caccgccagg gaatgggcaa tcgcacgtct ttgcactgac
      541 ttccggacac ggagcggcgc ggagatgacc ttcatcgggc agcacctccc ggaactggta
      601 ccgttcatgc aggaatgcta taccccccag gcagtaaacc aggcccgcaa ttcgttcaag
      661 aagaaggtct acaagtcggg agccacgttc ttctacgggg cgatgtgcgg gttcttcacg
      721 agcgaagagc ttgatgacct cctcttcgaa gcaagcgaag tagcccgttt tctcctggag
      781 gtagaacgga cgtcacttga tattgacgag gagatcgaga tcgaggaccg gatcacggac
      841 gtgatgcgaa gcgtggggga ggctgcggca cagatccttc gggccaggat gcacaatgag
      901 gaccagacag gagaagagcc cgaagttgcc gggaaccctc ccgaccagga gcaggtatga
      961 gggtaatcca cattgccgga gtctcgggat caggcaagac caccttcatc cggtcgctca
     1021 tccccctcct tgagcagaag ggccccaccg ccgttgcaaa gcacctgggg caccattgct
     1081 actccctcga gaaggggaag gataccacga tattcatgga agagggggca catgtctccg
     1141 caggggtaga catagagaag accgtggtgg tggcacgcgg gctctctttt ggagatatcc
     1201 tcccgctgct gtcatctatt ggtacagaat atttgctggt tgaggggtgg aagactcagc
     1261 cactcccgaa ggtggccatc ggtgacctgc cgggcgcagc agaagtgatt ctttccaacc
     1321 caaccgccgc ggaggtcatt gcatccctgg atctcttccc ccggtattac tctctggaag
     1381 ggcttgcaca aaaagtgcag gaaggatgca cggggggggg cgtggtgctg acaggccgat
     1441 acccggtgcc ggatcatgca ggtaacccgg agtcaagaag agagttttat ctccggtttt
     1501 cacccaccct acatgagata tcacgggttg cggaatcccg ccccggggga gtacggacca
     1561 tggtgcacct gcaccagggg ctcctcttcg gcggggagga tgggatcctc gtggcagtgg
     1621 gcgcaccaac tcccgcagat gccctggatg cgttctcatc ctgccatcgg cacctgctct
     1681 ccgcacttgg aaccggcagc ccgcaccagg gatgaacatg gaaggacaga aactcgagaa
     1741 aaagctcttc ggaaccaacg gcgtccgtgg agtggcggga agagagatga cccccgcgct
     1801 tgcgctctcg atcggagcag cactcggcac aatgcgccag ggcaccatcg ccgtgggcag
     1861 ggacacccgg acatcggggc cggcgcttgc atgcgccctg aaagcagggc tcctctcggc
     1921 agggtgtgac gtggtggatc tcggggtcct gcctacccct gcgctccagt atcttgtcag
     1981 ggaccacttc gacgcaggat cagtggtgac cgcatcgcac aacccccccg agtacaacgg
     2041 gattaaggtc gtggaggcgg acgggacaga gatgggcgac gagcagagca tcctcctcga
     2101 agaccatatc ttcaggggaa ctgcctacca ggccgcatgg gaccacgtgg gccgcgaaca
     2161 cgcggcgcca tacatggttg aggattacgt gcgggcagtc gcaggttcct tcccaaccgg
     2221 gaccggcggc gggacactgg tggcagcgga tcccggatgc ggggcggcgg cctggaccac
     2281 gcccgagatc ctcacccgga tgggatgccg cgtcttctct atcaacagcc agatggatgg
     2341 gaccttccca ggccggctgc ccgaaccatc ccctgagggg ctcgccccgc tcgccgacct
     2401 cgtgaaggca acgggggcac gcttcggggt ggcccatgac ggggacgccg accgggcggt
     2461 ctttatcgac gaccagggaa ggttcatcga agagaaccgg cagttcgggc tgatcgctga
     2521 cttcatctgc cggaatcgcc gcggggttgt ggtcacgccg gtcagcacgt caaggctgat
     2581 cgaggtgatc gcacatcgcc atggatgcag cgtggtctac accgcggtag gcagcatcta
     2641 tgtggccagg accatgatag ggctctcgaa acagggcact gcagtcgcat tcgggggcga
     2701 agggaacggc ggactcatat tccccgcgca ccagctctgc agggacgggg ggatgaccgc
     2761 agcgaccatg gtggcccttc ttgcagacac gtcgcaaccg ctctccaccc tcgtggatgc
     2821 gctcccaccg ttcttcatga ttaaagagaa gctgcagaag aaggatcaca tcgtgatc
//
LOCUS       DAOQVJ010000183         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_41297_length_2856_cov_3.160300, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000183 DAOQVJ010000000
VERSION     DAOQVJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41297_length_2856_cov_3.160300"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKL26_04755"
     CDS             <1..296
                     /locus_tag="PKL26_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNS83039.1"
                     /translation="GEFEECLALENEHEMALARHIIRFPMVIESVVRELKPHLLAAYA
                     RELADLFNAFYHFEPVLKSEGITRRSRLTLVKATQNTLAEALQTLGIDALRTM"
     gene            277..1170
                     /gene="twy1"
                     /locus_tag="PKL26_04760"
     CDS             277..1170
                     /gene="twy1"
                     /locus_tag="PKL26_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HNS83040.1"
                     /translation="MHSGPCDALRRQGYQFFSPDSSAALKPCLWCKRALRGGDMCYKH
                     QFYGIASHRCVQMTPTLRCNQRCLFCWRSFEHEYPGERDLSPDEILSMVKPLQKKALS
                     GYKPSPYVTPERFAEALDPKHVAISLSGEPTLYPHLPELIDLLTREGFSTFLVTNGTR
                     PDVVARCRPFQTYVSLAAPGIETYRKVCRPMEDTWEAIQQSLSLLGSRRSAIRITLVR
                     GYNDFSPDAYARMIQDSGASFVEVKGYMFLGYSRKRLERGNMPSFAHVKEFAEKIAAA
                     CDYEARDENPASRVVCLERIR"
     gene            1167..2753
                     /gene="sepS"
                     /locus_tag="PKL26_04765"
     CDS             1167..2753
                     /gene="sepS"
                     /locus_tag="PKL26_04765"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HNS83041.1"
                     /translation="MRFDPEEYRKKAREHFERAWHEGPSVLTPPSFGERYPRLAYCRA
                     AAHPIYEMVNRLRETYLSLGFTETCNPIIVDEQDVYRQFGPEASAVLDRVFYLGGLDR
                     PNQGIGREELEGISRITGKAVDPAAEERLREILQQYKKSTIDGDDLVHATSAALGIDD
                     GQTVEIYERVFSAFRELVPRSSRSTLRSHMTSGWFLTLSAIWEKYPLPLRLFSIDRCF
                     RREQEEGPTRLMSYHSASCVIAGEDITNEEGKAVSQALLSAFGYTDFRFRPDEKRSKY
                     YMPDSQTEVYARHPVHDWVEVATFGMYSPSALAEYGIGIPVMNLGLGVERLAMIAYNS
                     QDIRQLTYPQFHPRALTDLDIARAITVREEPSSMEGRRLARAIAETAREHATEPGPCA
                     FVAYDGPLLGILVRVTVEEQESGAKLCGPACGNEVFVHQGSVLGVPDTPQWAAVRTEG
                     TPAGISFLEAVSARAAARIEEAARCGKSAHVQVKMAKLPSDINLRIDEHAMRFITDQK
                     KKVDVRGPVFLSVRSDVTGG"
BASE COUNT          565 a          894 c          871 g          526 t
ORIGIN      
        1 ccggggagtt cgaagagtgc ctcgcgctcg agaacgagca cgagatggca ctcgcccggc
       61 atatcatccg gttcccgatg gtcatcgaga gcgtcgtccg cgagctgaaa ccgcacctcc
      121 tggcagcgta tgcaagagaa ctcgcggacc tcttcaatgc cttttaccac ttcgagccgg
      181 tgctgaaaag cgagggcatc acccggcggt cccgcctgac actggtgaag gcgactcaga
      241 atacccttgc agaggcgctg cagacacttg gcatcgatgc actcaggacc atgtgacgcc
      301 ctgcgccgcc aggggtacca gttcttctcc ccggattctt ctgccgccct gaagccctgc
      361 ctctggtgca agagggcact tcggggaggc gacatgtgtt acaagcacca gttctacggg
      421 attgcgagtc accgctgcgt ccagatgacc cccaccctcc ggtgcaacca gcggtgcctc
      481 ttctgctgga gatcgttcga gcacgaatat cccggggagc gggatctttc gcccgacgaa
      541 atcctctcga tggtaaagcc cctccagaag aaggcgctct cgggatacaa gccctcgccg
      601 tatgtcaccc cggaacggtt cgccgaggcc ctcgatccaa agcatgtcgc gatatcgctc
      661 tcgggcgagc ccacgctcta cccgcacctc cccgagctga tcgacctcct cacacgcgag
      721 ggattctcga cgttccttgt caccaacggt accaggcccg atgttgttgc caggtgcagg
      781 ccgttccaga catatgtctc gctcgccgca cccggtattg agacctacag gaaggtctgc
      841 cggcccatgg aagacacatg ggaggccata cagcagagcc tctcgctcct gggctcgcgg
      901 aggtcagcga tccgtatcac cctggtgagg ggatacaacg atttctctcc cgacgcgtat
      961 gcacggatga tccaggactc gggcgcatct ttcgtggagg tgaagggata tatgtttctc
     1021 ggatacagta gaaagagatt ggaaagaggg aatatgccga gctttgccca cgtaaaggag
     1081 tttgcagaga agattgccgc agcgtgtgac tacgaggcca gggacgagaa ccctgcaagc
     1141 cgcgtagtct gcctggagag gatccgatga ggtttgaccc tgaagagtac aggaaaaagg
     1201 caagggagca cttcgagcgg gcgtggcacg agggcccctc ggtgctcacc cctccttctt
     1261 tcggtgaacg gtacccgagg ctcgcctact gccgggctgc cgcccacccc atctacgaga
     1321 tggtgaatcg cctcagggag acttacctct ccctcggctt taccgaaacc tgcaacccca
     1381 taatcgtgga cgagcaggat gtgtaccgcc agttcgggcc cgaggcgagc gcagtgctcg
     1441 acagggtctt ctaccttggc gggcttgatc gccccaacca gggaatcggc cgggaggaac
     1501 tggagggcat ctcccgcatc acgggaaagg cggtcgatcc tgcagcagaa gagaggctcc
     1561 gtgagatact gcagcagtac aagaagtcca ccatcgatgg cgacgacctc gtccatgcca
     1621 cgtcggccgc actcgggatc gacgacgggc agactgtcga gatctatgaa cgcgtcttct
     1681 ctgccttccg cgagcttgtc ccccgttcct cccggagcac actccgcagc cacatgacca
     1741 gcggctggtt cctcaccctc tcggcgatct gggagaagta tcccctcccg ttgaggctct
     1801 tttcgatcga ccgctgtttc cggcgcgagc aggaggaggg gccgacgcgc ctcatgagct
     1861 accactccgc ctcctgcgtg atcgcggggg aggacatcac caacgaggaa ggcaaggctg
     1921 tctcccaggc cctcctctct gcgttcggat acacggactt ccgcttccgt cccgatgaga
     1981 agcggtccaa gtactacatg cctgactcgc agaccgaggt ctatgcccgc catcccgtcc
     2041 acgactgggt ggaggtggca actttcggga tgtactcccc atccgcgctc gcggaatacg
     2101 ggatcgggat cccggtaatg aacctcggtc tcggcgtgga gcgtctcgcg atgattgcgt
     2161 acaactccca ggatatcagg cagctcacct atccccagtt ccacccgcgg gcgctcactg
     2221 atctcgatat cgcccgggca atcacggtcc gggaggagcc gtcgtcgatg gaggggaggc
     2281 ggcttgcgcg tgccattgca gagactgcaa gggaacatgc cacagagccc gggccctgcg
     2341 cctttgtcgc ctatgacgga cctctccttg ggatcctggt cagggtgaca gtcgaagagc
     2401 aggagagcgg cgcaaagctg tgtggcccgg cttgcgggaa cgaggtcttt gtccaccagg
     2461 gttctgtgct cggggtgccc gacacccccc agtgggccgc ggtcaggacg gaagggactc
     2521 ctgcggggat ctccttcctt gaagcggtca gtgcgcgtgc ggccgcccgc atcgaggagg
     2581 cagcgcgctg cgggaaatcc gcacacgtgc aggtgaagat ggcgaagctc ccaagcgaca
     2641 tcaacctgcg catcgatgag catgccatga gattcatcac cgaccagaag aagaaggtcg
     2701 atgtccgcgg cccggtattc ctctcggtcc ggtcagatgt gacgggtggg tgatcctgga
     2761 gggccccgcc gctcctccag cgacctgctg tcgcgatacg tggaagaggg aacccgaaaa
     2821 gagcaagggg agttgccggg ttcctctgat atcatt
//
LOCUS       DAOQVJ010000184         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_41314_length_2855_cov_3.554286, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000184 DAOQVJ010000000
VERSION     DAOQVJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41314_length_2855_cov_3.554286"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..415
                     /locus_tag="PKL26_04770"
     CDS             35..415
                     /locus_tag="PKL26_04770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNS83042.1"
                     /translation="MTMEAQDTTLVEQDHLIGRVEEFKKKGYRLVQMSCTKVGEIYEI
                     NYSFDLHYTFQNLRIVVDGQTEVPSISGIYWGAFIYENEMHDLFGIRIVGMNIDFGGN
                     LFRTAKKNPFANPTFREEGPCHGE"
     gene            400..1479
                     /locus_tag="PKL26_04775"
     CDS             400..1479
                     /locus_tag="PKL26_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNS83043.1"
                     /translation="MSRRIVVPFGPQHPVLPEPIHLDLVLQDERVVEAIPSIGYIHRG
                     LETLVAKREYPEYVYIAERTCGICSFIHGMTYCQAVEQVMGLEVPERALYLRTIWSEY
                     SRMHSHLLWLGLFADAFGFENLFMASWRLREFVLDVLEETTGGRVIQGSCKVGGVRRD
                     IPADTLDRMVGDLADFERNYREIANVFLRDESVIGRTRGVGVLSRDDAYYLGAMGPTA
                     RGSGVAIDLRETGYAAYGKINFKPVVEAGGDCYARCAVRVKEIFSSIDIVRQAAARIP
                     EGEIEVKPKGNPDGECFSRAEQPRGELVHYVKGDGSRHLVRSRIRTPTLTNIPPLVKM
                     LAGCELADVPVIVLSIDPCIGCMER"
     gene            1487..1888
                     /locus_tag="PKL26_04780"
     CDS             1487..1888
                     /locus_tag="PKL26_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS83044.1"
                     /translation="MAFFEMAKTAILTTLKRPATRRYPAVPSKVTAISRGKVTIDPSR
                     CISCGMCMKKCPSGAICVVKDEKTWSIDRLKCVVCNCCVEVCPAHCLSMETGYSPSMT
                     THEGIEVVHITYVKPERPARKEPEEKSEQGA"
     gene            complement(2143..2385)
                     /locus_tag="PKL26_04785"
     CDS             complement(2143..2385)
                     /locus_tag="PKL26_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83045.1"
                     /translation="MIGLLCDIDYERVEGYISRHGEGGYQMLKDRGIDEDIAGHVRRH
                     NDFLFGASEDTVDVALQAAGNISGLVNRVRSGERRS"
     gene            complement(2486..>2855)
                     /locus_tag="PKL26_04790"
     CDS             complement(2486..>2855)
                     /locus_tag="PKL26_04790"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNS83046.1"
                     /translation="LGYHLQIPSIGIAGRGTRGMEAREPSRSRGSSTPVWMDGERVGT
                     LLRTREGSAAVYVSAGYRTTLEQAGEIVMQCIRDNRFPEPLVQADRAARRMRKRCERY
                     YDRMRESGLHAERGRPLPDV"
BASE COUNT          621 a          819 c          806 g          609 t
ORIGIN      
        1 taaaacctga acgggctggt tccggaggtg agagatgaca atggaagcac aggataccac
       61 actggtagag caggaccacc tgatcggcag agtggaagaa ttcaagaaaa agggataccg
      121 gctggtacag atgagctgca ccaaggtcgg ggaaatatac gagatcaact actcctttga
      181 cttacactat acgttccaga accttcgtat cgtggtggac gggcagacag aagtccccag
      241 tatttccggg atatactggg gggcgttcat ctacgagaac gagatgcatg acctctttgg
      301 tatacggatc gtgggaatga acatcgactt tggcggaaac ctgttccgaa cggcgaagaa
      361 gaacccattt gcgaatccca ctttcaggga ggagggacca tgtcacggag aatagtcgtg
      421 ccgttcgggc cgcagcaccc cgtactcccc gagcccatcc accttgacct ggtactacag
      481 gacgagaggg tggtagaggc gatcccgtcg atcggataca tccacagggg cctcgagacc
      541 ctggtggcaa aacgcgagta tcccgaatac gtctatatcg ccgagaggac ctgcggtatc
      601 tgcagcttca tccacgggat gacctattgc caggcagtcg aacaggtaat gggcctcgaa
      661 gtgcccgaac gtgccctcta tctcaggacc atctggtcag agtattccag gatgcactcg
      721 cacctcctgt ggctcgggct ctttgccgat gcgtttggtt ttgagaacct tttcatggcc
      781 tcgtggagac tgcgggagtt cgtcctcgat gtcctggagg agacaaccgg cgggcgtgtc
      841 atccagggaa gctgcaaggt gggaggagta aggagggaca tcccggctga taccctcgac
      901 cgtatggttg gggaccttgc tgacttcgaa cgcaattaca gggagatcgc caatgtgttc
      961 cttcgcgacg aatccgtcat cggccgtaca cgcggagtcg gggtgctctc acgtgatgat
     1021 gcgtactacc tcggtgcgat gggccccacc gcgcggggca gtggtgtggc catagacctg
     1081 agggagacag gatacgcggc ctatggaaag atcaacttca aacccgtggt ggaagcgggt
     1141 ggagactgct atgcgcggtg tgccgtgagg gtgaaggaga tcttttcctc gatcgacatc
     1201 gtgcgccagg ctgctgcccg tatccccgaa ggggagatcg aggtcaagcc gaagggaaat
     1261 ccggatggcg agtgcttctc acgcgccgag cagccccgag gggaactcgt gcactacgtc
     1321 aagggtgacg gaagccggca cctggtgcgc tcccgaatcc gcactccgac actcacgaac
     1381 ataccgcccc tggtgaagat gcttgcaggg tgcgagcttg ccgatgtacc ggtaatcgtg
     1441 ttgagtattg acccatgtat cgggtgcatg gagaggtgag aagaggatgg cattctttga
     1501 aatggcaaaa accgcaatcc ttaccaccct gaagaggccg gcaaccaggc ggtatcccgc
     1561 agtcccttcg aaggtgactg ccatctcccg gggtaaggtc acgattgatc cttcacgctg
     1621 catctcatgc ggcatgtgca tgaaaaaatg cccttcaggt gccatatgcg tggtcaagga
     1681 cgagaagacc tggagtatcg accgcctgaa gtgcgtggtc tgcaactgct gcgtggaggt
     1741 atgccccgcc cactgtttga gcatggaaac aggctattcc cccagcatga caacccatga
     1801 gggcattgag gtagtgcaca tcacctatgt caagccggaa cggcctgccc gcaaagaacc
     1861 ggaagaaaag agcgagcagg gggcctgagc gggggacagc tttcgtttcg tatttttttt
     1921 cttccgcact ccacttaagc cggacaggtg aatcggctgg cgcatccttt cattcgagcc
     1981 cgagatcgcc acgcatctcc tgcatggcgc caagagccag tgaaaagaac tccccgagtg
     2041 ccatgaaccg ggtgatcccc atcaccttct ctcttctgta tcctgcagca aatgcctttt
     2101 ccttgaactt tttctggagg gtcttttcag ttacctcagt aattacgacc tcctttcacc
     2161 gctgcggacg cgattgacca gccccgagat gttaccagct gcctggaggg ccacgtcaac
     2221 agtatcttcg gacgccccga agaggaagtc attgtgcctc cggacatggc ctgcgatatc
     2281 ctcgtcgata ccccgatcct tcagcatttg gtatcctccc tcgccgtgcc tgcttatgta
     2341 gccttccaca cgctcgtaat caatatcgca aaggagcccg atcatctccc agcactgcgg
     2401 gtctcctcac atttgtctgg caagtgcctt catgatcgcc gccgtagcaa gacagtgccc
     2461 gcggaggctc ttttttttaa ttgactcaca catcaggcaa cgggcgtcct ctctctgcat
     2521 gcaaaccaga ctctctcatc cgatcgtaat atctctcgca tcgcttgcgc atccttctgg
     2581 ctgcgcgatc cgcctgcacc aatggctccg ggaaccgatt gtcccggatg cactgcatga
     2641 cgatctcccc ggcctgttca agggtagtgc ggtatcctgc cgagacatag actgcggcac
     2701 tcccttcccg tgtcctcagg agcgtgccca ccctctcccc atccatccat actggtgtcg
     2761 atgacccccg cgaacgcgag ggttcccggg cttccatgcc tctcgtacct ctgccagcta
     2821 tcccgatgct tggtatctgc aggtgatagc caagg
//
LOCUS       DAOQVJ010000185         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_41633_length_2842_cov_4.105490, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000185 DAOQVJ010000000
VERSION     DAOQVJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41633_length_2842_cov_4.105490"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..727
                     /locus_tag="PKL26_04795"
     CDS             152..727
                     /locus_tag="PKL26_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83047.1"
                     /translation="MDISPKTTLGVLLLVLTAILFFAGCTQPSPVTQTTPAPTATTAA
                     PTLTPTANSNETMKAEIAAFAGTFASETDGSTLLAAFNEGPNSTAFAELLGQLKAFKE
                     SDPRIKYVYTLEQRDGTVRFIVDANYGMPDGSGYLEVYDDAPVELKTPVTAPIGAGPY
                     TDKWGTFYSGYAPVDAGSNATVILIGVDVVA"
     gene            863..1234
                     /locus_tag="PKL26_04800"
     CDS             863..1234
                     /locus_tag="PKL26_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS83048.1"
                     /translation="MPKVLIIDDSGFQRKIITSILEAEGNMVLTAENGRTGFDIAVKE
                     SPDLIICDLLMPELDGYGLLRLVRDHKLAIPVLILTSDIQNTTRTECLALGALDVLNK
                     PVKKETLVPIIKDALRSAKRP"
     gene            1231..1839
                     /locus_tag="PKL26_04805"
     CDS             1231..1839
                     /locus_tag="PKL26_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNS83049.1"
                     /translation="MNIDEDSVDAIRELINIGVGRAAGLLNEMTGTHIRLMVPEVRIL
                     RYADLVSENRILEQDRLSAVTLKFEGSFSGISMLVFPPDSAAILITALTGEEIRAPDL
                     DALRIETLNEVGNIVNNAVMGSITNVLNERLTYSMPTYLEGSIAQILGNRRAIDYDWV
                     ILAISQFSLADLQVVGTILMIFEIGSLDSLVKRLQDAAGQER"
     gene            1836..>2842
                     /locus_tag="PKL26_04810"
     CDS             1836..>2842
                     /locus_tag="PKL26_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS83050.1"
                     /translation="MSPPPPLPSGNFFDIIPLGVVILAPDYTVVSWNRTIAEWTGIPR
                     NEILGNDIRERFSHLKDRRYNIRIQQVFDGGPAAFFSTQFHPHFIPAPLPGGGLRFQR
                     TSVHSLQEGGETYAILFIDDVTELVAQVRAFREMKDRALAEVDERTRKEAALALANKK
                     IGLLSSITRHDILNQVMALKFYIETMREETNPQQVAALLAKEMVVANAIERQIHFARD
                     YQEMGIHAPGWQRVASIVERSKEALPTGNISVVANVGSIEVYADPLLEKVFYNLIDNA
                     LQYGGPTMSTIRISTNENGDGLVLTIEDDGSGISEMDKPHLFTRGYGKHTGFGLFLSR
                     E"
BASE COUNT          663 a          824 c          764 g          591 t
ORIGIN      
        1 tcctcatccc gggctggtgg ttcttgaagg ccgcaatgcg gcggtgggac gaagtagacc
       61 cggccggatt cgggtagagg gatccctctc gtattttttt attgcgaata taccaaaatc
      121 cctatattga aggcccccca tgaactcttc catggacatt agtcccaaaa caacactcgg
      181 agtactgctc ctcgtgctta ccgcaatcct cttcttcgct ggatgtaccc agccttctcc
      241 cgttacccag accactcctg cgcctactgc cacgactgct gcacccacgc tcactccgac
      301 agctaattca aacgagacta tgaaagccga aatcgccgca ttcgcaggaa cgttcgcctc
      361 tgaaaccgac gggagtacgc tcctggccgc attcaacgag ggtccgaact cgactgcatt
      421 cgcagaattg ctcgggcagc tgaaagcatt caaggagagc gatccccgaa tcaagtatgt
      481 ctatacgctc gagcagcggg acggaacggt caggttcatc gtcgacgcga actatggcat
      541 gcctgacggt tcaggttacc tcgaggtata tgatgatgcc cccgtggaac tgaagacccc
      601 agtcaccgct ccgattgggg cagggcccta tacggacaag tggggaacat tctattcggg
      661 ctatgcaccc gttgatgccg gttcgaacgc gaccgtgatt ctcatcgggg tcgatgtggt
      721 agcctgatcc agcactccct ccttttttca ttcatttcgc cctgggccct gcacggggtc
      781 gccatccttc agtgggctgt gcagtcgata aagagataaa caggattgaa tgatacaatc
      841 ccatagcaaa ggagatatcg cgatgccaaa ggtcctgatc attgacgact cgggctttca
      901 gcgaaaaatc atcacctcga tcctggaagc agaaggcaat atggtcctca ccgctgagaa
      961 tggaagaact ggttttgaca ttgcggttaa ggagtctccg gatctcatca tctgcgacct
     1021 cctgatgccc gaacttgacg ggtacggact gctgaggctg gtaagagatc acaagctggc
     1081 cattccggtg ctaatcctga cctcggatat ccagaatacg acaagaactg agtgccttgc
     1141 ccttggtgcc ctcgatgtcc tgaacaagcc ggtaaagaag gagacccttg ttcccataat
     1201 aaaggacgcg ctcaggagcg cgaaacggcc atgaacatcg acgaggattc ggttgatgcc
     1261 atccgtgaac tgatcaacat tggggtcggc cgggctgcag ggctcctcaa tgagatgacc
     1321 gggacccaca tccggctgat ggtcccggag gtgagaatac tccggtatgc agatctcgtc
     1381 agtgaaaacc ggatcctgga acaagaccga ctttctgcag tcacccttaa attcgagggt
     1441 tcgttctcgg ggatcagcat gctggtgttt ccgccggaca gtgctgcaat cctgattacc
     1501 gcgctcacag gggaggagat cagggcgccg gacctcgacg cgctcaggat cgagacactg
     1561 aacgaagtcg gaaacatcgt gaacaatgcg gtcatggggt ctatcacaaa cgtcctgaac
     1621 gaacggctca cctacagcat gcccacatac ctcgagggca gcattgccca gatccttggg
     1681 aaccgcagag ccatcgacta cgactgggtt atccttgcga tatcgcagtt ttctctcgcg
     1741 gacctgcagg tcgtgggaac gattctgatg atattcgaga tcgggtcact tgattccctg
     1801 gtaaagaggc tccaggacgc ggcaggccag gagagatgag tccccctccc ccactcccct
     1861 ctggaaattt ttttgatatc atccccctgg gagtggtgat cctggcaccg gactacactg
     1921 tcgtttcatg gaacaggacc attgcggaat ggacgggaat tcccaggaat gagatactcg
     1981 gaaacgacat acgggaacgg ttttcccacc taaaagaccg gcgctacaat atccgcatcc
     2041 agcaggtatt cgacgggggg cctgcggcat tcttctcgac gcagttccac ccccatttta
     2101 tccccgcacc cctccccggt ggagggctcc gcttccagcg gacgtcggtc cactccctgc
     2161 aggaaggagg agagacctac gcaatcctct tcatcgacga cgtgaccgag cttgtcgcac
     2221 aggtccgtgc attccgggag atgaaagacc gtgccctcgc ggaggtcgat gagcgcacga
     2281 ggaaagaggc ggcacttgca cttgccaaca agaagatcgg cctgctctcg agtattactc
     2341 gccacgacat cctgaatcag gtcatggccc tgaagtttta tatcgagact atgcgtgaag
     2401 agaccaatcc ccagcaggtt gcggctctcc tcgcaaagga gatggtggtt gcaaatgcca
     2461 tcgagcgcca gatccacttc gcacgcgact accaggagat gggcatccat gccccgggct
     2521 ggcagcgcgt agcctcgatt gtcgagcggt caaaagaggc cctccccaca ggcaacatca
     2581 gcgtcgttgc caatgtcggc tccatcgagg tatacgccga cccgctcttg gagaaagtgt
     2641 tctacaacct catcgataac gctctccagt acggcggccc caccatgagc accatcagga
     2701 tctcgacaaa cgagaacggg gacggcctcg tcctcacaat cgaggatgac ggctcaggaa
     2761 tctccgaaat ggacaagccg catctcttca cacgcggcta tgggaagcac acgggttttg
     2821 ggctttttct cagccgcgag at
//
LOCUS       DAOQVJ010000186         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_42053_length_2822_cov_3.851102, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000186 DAOQVJ010000000
VERSION     DAOQVJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42053_length_2822_cov_3.851102"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1541)
                     /gene="nrdD"
                     /locus_tag="PKL26_04815"
                     /pseudo
     CDS             complement(1..1541)
                     /gene="nrdD"
                     /locus_tag="PKL26_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
     gene            complement(1815..2099)
                     /locus_tag="PKL26_04820"
                     /pseudo
     CDS             complement(1815..2099)
                     /locus_tag="PKL26_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025881.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
BASE COUNT          562 a          821 c          834 g          605 t
ORIGIN      
        1 tcgcgggtaa atgcaaaata cccgatctgt gtggtcctgc agaggttcat cgccatctcc
       61 atcaggccct ttggattcgg cctcgactcg ccaagccaga tatggaagat gttgccgccg
      121 tccacaatag ggaagaagac atgctcgatg gacatccgct ccgccatggt gacatttgca
      181 ccgggggcca cgtgggtccc gttcgtgtag tatatgggca ggtcccttgt agaaccgaga
      241 cgtggcaggg ctttctcgag gtcccccttg atcaccttca tcgcaccgcc tcggtactcc
      301 ttgttgagga gatccgcgac cgcgaaccgc tgcccggtcg tctcggcagg ggtgcgggcg
      361 agtgcgatgg tcatgttgtg ccttttggaa agctcgcggg catactgctc catgtgggtc
      421 attgcaagga ttgccagcct gaaggcatcc cgcgattcat gaagctgcga cccgacgtgg
      481 tgctgcacca tctcgtttac tcccaccact ccgatcgtgt agaccaggcc atccaggtcg
      541 actgcaaccg atcctcgctc ccccgtgaca ggatccttcg ggcgctgcat cgcaaacggc
      601 atcctcccct gggaccggat cgtctcgagc cacctccgct tgatgcggaa gacctcgaca
      661 cacacgtcca tcagtgcccg gagctctgaa aagagccgct cgttgtcccc tcccgcacgg
      721 taggcggccc tcgggcagtt gagggagacc acctgccagg agcccatcga gaaatgcttg
      781 ccatcctgga aatacagttt ctgatcgaac tcggcgtcct ggtcggcgac agcagagaac
      841 tggtaggcac agcactggta gcatgagatc cccttgccgg cccccctgta gggggggatc
      901 tggttgtcat agtagggggt gccgaacttc gatgccagtt cgaacgtgag gaggtagagg
      961 tcctggtagc tgggaaggtc ggggtgcgcc tcgttgtatg cctggtcctc ctgcatgaag
     1021 tcgggctcga tgctgatctc gggttttggg aaactgaacg gcttgcccca ggcgtccccc
     1081 tcgagcatga cctccatcag tgccttgaag aggaggcgta cctcccgttc gaactccccg
     1141 tatgtcctgc gggggccgct gctgccctcc cagaccctgc cctggaagac gaccggcttg
     1201 tccttccaga gagagggcac acccgggctg agctggaccg atgagaagac gagctgtccg
     1261 cctcttgcga ccatcatctg ggtcatctcg tagacaaaca tctgcatcag ctgcttgatc
     1321 tccccgtatt ccatcccttc aaagtagggg gcgaggaacg tgaggaagtt gtaatatccc
     1381 tgtccgccgg caaagttggt ctgggcgctc ccgagcgctt tgactgcgtg gagcaccgcc
     1441 acctccgcac gcttcgcagg cccggcaacc gatgccttgg tcccgttccc gtcgggcatc
     1501 aggccgtagt agaagaagta gcggaggtcc cagtcctggc agttgtggat caggagacca
     1561 tcggcggcat aataggtatg gctctcatcg gatccgtctc cttcaaggaa aatgtcgtat
     1621 acaaattccc cggtagagtc aacctcttcg attgagacga tcatttcagg ttccgtattt
     1681 cgggagatgg cttttacgcg ggagaggaac gcattgatat gctcctggcg gtcgtcgttg
     1741 aaacgggcat atcttgagaa gatacggata tttaccgccc cgttcatctg gatgcgatat
     1801 atggtggtcc ggtccttttc ttcgtatagt tcaacgatat gcggcttcat ccccagtgac
     1861 gataggagga gggaaagttc ctggcggagg gcgggcgaag aggtggtata attgacgatg
     1921 cagtcggatt tctccgggcg gtaatagact gatccgtctc ccgtgaagag cgcggagagg
     1981 aactcggcca ggagctcgtc atcaaggtgc cagacgatcc aggggaggcg cttattcaca
     2041 gagttttccg ggacgccaaa cacgtaacgg aagaggagcc agaggagctt gccacacatg
     2101 tacacctgca tggcccttgg ccgcgtgacc ggaacgccgt aaatctcggt tgaggcgatt
     2161 gcaccccctg tcctcgtggc gcagcactgc agggaagcat gggcgtcagc gatgattgcc
     2221 gacgactggt ggttctcggt gatgacaaga ttgtaatgct ttccatcact cacattgtag
     2281 ttcccctctg caacgaaata accaaggagc cggacaaggg agggatcgat cggcaactcc
     2341 aggggaaggt catgttcgct tccggcagca ccgatcctga tgccggagag gtcagtaatg
     2401 tcgaacctct cgcagaggcg atggaagact gcaacaggca ttatcccccg gctgtaccac
     2461 tgcttccggt ggggcacccc gagttcccgg gagatctcgg tatactttat cccctcttcg
     2521 tgcaggcggg aaaagatctc ccctgctccc ctgacataca cattcccaag gtgatcagga
     2581 ggtacccgcg tgacgagttc gtggaggagg tgaattgagt agaccggttc ccccctgatc
     2641 tcgcgattct ctgggaggga aaagagcctg tctccaacgt tcagggtatc tgcacggacg
     2701 accttcatcc ccgcttcatt gacagggatg cggtgttccc ccgttacccg aagagccctt
     2761 cccgacccgg tcctgatctt cagcagccgc tctcccctta caggccgcct ggtgagtttg
     2821 cg
//
LOCUS       DAOQVJ010000187         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_42284_length_2811_cov_4.567126, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000187 DAOQVJ010000000
VERSION     DAOQVJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42284_length_2811_cov_4.567126"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKL26_04825"
     CDS             complement(<1..565)
                     /locus_tag="PKL26_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83051.1"
                     /translation="MSANSSQNPDHVRKKREIQLALAVPLCAFGGLILAILLKEAGLI
                     ADAADFYWGCVAASLILAYLAYIKPRKDLVSLFAPAYALLAFVIPLETKASFLLQILY
                     AVSITLLVIRLHYRFSTPKTIAKEEDSMEKYLYNYIHRMTPLFRGIDPETAHEIASAV
                     LSFKFGLYAKTVTDAGKASSRIPKDGAG"
     gene            701..1879
                     /locus_tag="PKL26_04830"
     CDS             701..1879
                     /locus_tag="PKL26_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNS83052.1"
                     /translation="MKVCIMCGGEGTRLRPLTFERPKPCIPIVNRPSIQHLVSHLSNL
                     GFREIVVTLGYMGDAIEDALGDGSLFGADITYVHEKIKLGTAGSVKNAQAYLEEGDFL
                     VVGGDHVTDLNLLDFYREHSRHQAPVSIGLISIDEPGEYGIAEIDADFQIKRFKEKPA
                     PGEIFSNLASTGIYVCRPEIFDEIPLGRKFDFARDLFPHLMESGHVLRAWLARGNWTD
                     VGNPASLRQAERWKLQEIPYTSISGDLTIRAGQIQGPVQLGTSFSMGVNSRIIGPVAI
                     APGTAIGDHVLIGPYTSIGENCLVKSNAKIFSSSLYNRVVVGRNSTVSGSIIDNDTVI
                     GDDCSLENDTVIGPRVVFRNGVVVHSRTRIWPEVVVPDGTVVKEHLLNDEYAVKCEGS
                     "
     gene            complement(1876..2661)
                     /locus_tag="PKL26_04835"
     CDS             complement(1876..2661)
                     /locus_tag="PKL26_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS83053.1"
                     /translation="MDKKKVRDYMTYDVVTVDAQGTVREVMEKIQKTGHDGFPVVMGN
                     EVVGYVAARDLLFQPVTTPVRKVMSEHLIVADPDMSINDAARVIFRSGIQKLPVVDEQ
                     NRLLGIISNTDVIRSQIEHVSPEKVFNFITTLKTLHGISPELKREVVPIDQLLPTQSR
                     IYEDELEGRIYEIKKGLAEPLIVVRRPNKLILVDGHHRAIAARKMGIKTLDAYVIEVN
                     EDIELGMERTARAMNLRSLEDIQVLDYARHPLVAITHRLVRKG"
BASE COUNT          603 a          765 c          829 g          614 t
ORIGIN      
        1 cgccggcccc gtcttttggg attcgggaag aagcttttcc ggcatcggtt actgtctttg
       61 catacagccc aaacttgaag gagaggacag ctgatgcaat ctcgtgcgca gtctccgggt
      121 caatgccccg gaagagaggg gtcatgcgat gtatataatt gtacaggtat ttttccatgg
      181 agtcttcctc tttggcaatg gtttttggag tgctgaagcg gtaatgaagc ctgatcacca
      241 ggagcgtgat actcacggca tacagtatct ggaggaggaa ggacgccttc gtctcaaggg
      301 gaattacaaa ggcgaggagt gcatatgccg gtgcgaagag agagacgagg tctttgcggg
      361 gctttatgta tgcgagatac gccaggatca gcgacgcggc aacgcacccc cagtaaaagt
      421 cggccgcatc cgcgatgagg cccgcctcct tgagaaggat tgcgagtatg agacctccga
      481 atgcacagag cggcacggca agggcgagct ggatctcacg cttcttcctt acatggtcag
      541 ggttctgtga tgaattggct gacaagatac cattcagatg tggggacccc accagaaaaa
      601 gatttcaata tgcccccggg agtctgctca tttgtcctgg cagaccaaag aacgccattc
      661 tcccagatgg tttaaacgta tgcaggtgga accaatggat atgaaggtgt gcatcatgtg
      721 cgggggagag gggacacgcc tccgccccct cacgttcgag cgccccaagc cctgcattcc
      781 catcgtgaac cgcccctcga tacagcacct tgtctctcac ctctccaacc tcgggttccg
      841 cgagatcgtg gtcaccctcg ggtacatggg ggatgcaatc gaggatgcgc tgggcgacgg
      901 gtcgctcttt ggggccgata tcacctacgt gcacgagaag atcaagctcg ggactgcagg
      961 gagcgtgaag aatgcccagg cataccttga agaaggagat ttcctcgtcg tgggtggaga
     1021 ccatgtcacc gatctcaacc tccttgattt ctaccgggag cattcccggc accaggcgcc
     1081 ggtctccatc gggctgatca gcatcgatga acccggagaa tacgggattg cggagatcga
     1141 tgcggacttc cagatcaaga ggttcaagga gaaaccggcg ccgggagaga tcttctcgaa
     1201 tctcgccagc accgggatat atgtctgcag gccggagatc ttcgatgaga tccctctggg
     1261 aaggaagttt gactttgcca gggatctctt cccgcacctc atggagtccg gccatgtgct
     1321 ccgggcctgg ctcgcacggg ggaactggac cgacgtgggg aaccctgcat cactccgcca
     1381 ggctgaacgc tggaaactgc aggagatccc gtatacgagt atcagcggcg atctcacgat
     1441 cagggccggg cagatccagg ggccggtcca gctcgggacc tcattctcaa tgggtgtcaa
     1501 ttcgcgaatc atcggcccgg tggccatcgc tcctgggacc gccattggtg accatgtcct
     1561 catcggcccc tacacgagta tcggtgagaa ttgccttgtg aagagcaacg cgaagatatt
     1621 ctcctcctcc ctttacaacc gggtggtggt agggaggaac agcaccgtgt caggaagcat
     1681 catcgacaat gacacggtga ttggggacga ctgcagcctc gagaacgaca cggtcatcgg
     1741 gccgagggta gtgttcagga acggggtggt cgtccattca aggacgagga tctggcccga
     1801 ggtggtggtg ccggacggaa cagtcgtaaa agaacacctc ctgaatgacg aatacgcggt
     1861 gaaatgcgag gggtcctagc ccttccggac caggcggtgc gtgatggcga ccagaggatg
     1921 gcgggcgtag tcgaggacct gtatatcttc aagcgatcgc aggttcatgg cccgggcggt
     1981 cctctccatg ccgagttcga tatcctcgtt cacctcgatg acataggcat cgagtgtctt
     2041 aattcccatc ttccgggcgg caatcgccct gtggtgcccg tcgacaagga tgagcttatt
     2101 cggcctcctc acgacaatga ggggttcggc gagtcctttt tttatctcgt agatgcgccc
     2161 ctcgagttcg tcttcgtaaa tgcgtgactg ggtgggaagg agctggtcta tcgggacgac
     2221 ttcccgcttg agctcagggg agatcccatg gagcgtcttt aacgtcgtga tgaagttgaa
     2281 gaccttctct ggggagacat gctctatctg ggatcggatc acgtcagtat tggatatgat
     2341 tccaagcagc ctgttctgct cgtctaccac ggggagtttc tggatgccgg aacggaagat
     2401 gaccctcgct gcatcgttga tgctcatgtc agggtcagcc acgatcaggt gctcggacat
     2461 caccttccgg acgggagtcg taactggctg aaagaggagg tccctggcgg ccacgtaacc
     2521 caccacctcg tttcccatca cgacagggaa cccgtcatgg ccggtcttct ggatcttctc
     2581 catgacctcc cgcacggtac cctgggcatc cacggtgacg acgtcgtagg tcatgtagtc
     2641 cctgaccttc ttcttatcca tcgtacctca tttcagggtc aaaagactaa aaaccatcgg
     2701 aaaaaaaggg agattattcg atttttatgc gggtcccccg cgcttccttc gatttcttca
     2761 ttcggatctc gagcaccccg ttcttgaagc tcgcggtggc ccccttctcc g
//
LOCUS       DAOQVJ010000188         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_42476_length_2803_cov_4.614265, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000188 DAOQVJ010000000
VERSION     DAOQVJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42476_length_2803_cov_4.614265"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKL26_04840"
     CDS             complement(<1..842)
                     /locus_tag="PKL26_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HNS83054.1"
                     /translation="MDPGFDAAIGKADLPDKTKQQLRTFLEGKEISKEQFSTIIDRVI
                     NEYMNTRIEACEAVGIIAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDAR
                     KEPSTPTMTIYLQEDYSGDRDRAREVSWQIEAAPLHEFGDIVIDMENMQIVVQLNTAV
                     CDKRKISVAEIVEIAPRKIRDRRHYRDFDHEVDEKGAIMYFMPKDRESYQNLFQLAEH
                     VRNVIVQGIDDIERVVVRKESGEYILYTEGSNLKDVFEVVGVDTTRTRTNNISEISQV
                     LGIEA"
     gene            complement(844..>2803)
                     /locus_tag="PKL26_04845"
     CDS             complement(844..>2803)
                     /locus_tag="PKL26_04845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HNS83055.1"
                     /translation="EDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVTT
                     YLDNEVAGCPPARHRSGRPLKTLSQRLKGKDGRFRGSLSGKRVNFSARTVISPDPNLS
                     VIEVGIPLAVANEMTIPVQTNPFNIEELRQMVRYGPFRARLSDPCGANYVIRPDKRRI
                     RLAEGNLETVAEMLEPGWTVERQIRDGDIVLFNRQPSLHRMSIMAHRVKVMDGRTFRL
                     NPAVCPPYNADFDGDEMNLHIPQTEEARAEAAILVAVEENILSPRFGGPIIGGIHDHV
                     SGIFMLTNQLKWHTKEEALYLLKNIGLEHLPAPGMEENGQELWSNKQIFSTVLPKELN
                     MVFRASSCQNCETCKRQLCERDAYVRIIDGILESGTIDKKAIGAFDGQIVNRIIRQYG
                     MKRAAEFIDAITHLSIRAIMLDGFSFGIDDEDLSKTEYGQIDEVLSNAVNDVQRRIKI
                     YEDGQLEAMPGRTPEETLEMQIMQVLGKARDRTGEIAGRHLGLGNSAVVMAVSGARGS
                     MLNLTQMAGCVGQQSVRGERIMRGYDDRTLPHFKKGDRGADAHGFITHSYKSGLNPTE
                     FFFHAIGGREGLVDTAVRTSQSGYLQRRMINALQDLKVAYDGTVRTTGGRIIQFCYGE
                     DGTDPMKSSFGDPVDVKGIVESILKEEV"
BASE COUNT          481 a          853 c          785 g          684 t
ORIGIN      
        1 gcttcgatgc cgagcacctg ggagatctcg ctgatgttgt tggtgcgggt cctcgtggta
       61 tccacaccga caacctcaaa cacgtcctta aggttggagc cctcagtata taggatatac
      121 tctccactct cctttcttac caccacgcgc tcgatatcgt caatcccctg gactatgacg
      181 ttgcgcacgt gttcagcgag ctggaagagg ttctggtaac tctccctgtc ctttggcatg
      241 aagtacatga tcgcgccctt ctcgtccacc tcgtggtcga aatcccggta atggcgccga
      301 tcccggatct tgcgcggtgc gatctcaaca atctctgcaa cggagatctt cctcttgtcg
      361 cagaccgcgg tgttgagttg caccacgatc tgcatgttct ccatgtcgat gacgatatcc
      421 ccaaactcgt gcaggggcgc ggcttcgatc tgccagctca cctcccgcgc acggtcgcga
      481 tctcctgagt aatcctcctg gagatagatt gtcatggtgg gggtgctcgg ctccttcctg
      541 gcatccatga tctcaattag cctgggcagg ccgagtgtca cgttgatctc agctacaccg
      601 gcatagtgga acgtacgcat cgtcatctgc gtgccaggct ctcctatcga ctgcgccgcg
      661 atgatcccga ccgcctcgca ggcctcgatg cgggtgttca tgtactcgtt gatcaccctg
      721 tcgatgatgg ttgagaactg ctcttttgaa atttccttgc cttcaaggaa tgtgcgaagc
      781 tgctgcttgg tcttgtcagg taggtctgcc ttcccgattg cagcatcaaa cccgggatcc
      841 atctcacacc tcctctttca ggatactttc cacgatccct ttcacgtcca caggatcgcc
      901 gaaactgctc ttcatggggt ccgtcccgtc ctcaccgtag cagaactgga tgatacgccc
      961 tcctgttgtc ctgacggtcc cgtcgtatgc gaccttcagg tcctggaggg cgttgatcat
     1021 acgcctctgc aggtacccgc tctgcgatgt cctgactgca gtgtccacga gaccttcgcg
     1081 gcctccgatg gcgtggaaaa agaactccgt gggattcagc cctgacttgt agctgtgagt
     1141 gatgaaaccg tgggcatctg cgcccctgtc gccctttttg aagtggggaa gcgtccggtc
     1201 gtcgtaccct ctcatgatac gttctccacg gacagactgc tgcccgacgc accctgccat
     1261 ctgggtaagg ttcagcatgg agccccttgc tcctgatact gccatgacca cggcgctgtt
     1321 cccgagacca aggtgccggc cggcaatctc accggtccgg tcacgggcct tcccgagcac
     1381 ctgcatgatc tgcatctcga gggtctcctc gggagtcctc ccgggcattg cctcgagctg
     1441 cccgtcctcg tagatcttga tccgccgctg cacatcgttg actgcattcg agagcacctc
     1501 atcgatctgg ccgtactctg tcttcgagag atcctcgtcg tcgatgccga acgagaaccc
     1561 gtccagcatg atggcacgga tcgagaggtg ggtgatggcg tcgatgaact cggctgcccg
     1621 cttcatgccg tactgccgga tgatgcggtt cacgatctgc ccgtcgaacg ccccgatggc
     1681 cttcttgtcg atggtccctg attcgagaat cccgtcgata atccggacat atgcatcgcg
     1741 ttcgcaaagt tgtctcttgc aggtctcgca gttctggcag ctgctcgccc ggaacaccat
     1801 gttcagttcc ttcggcagca cagtggagaa gatctgcttg ttgctccaga gttcctgccc
     1861 gttctcctcc attccgggtg cagggagatg ttccaagccg atattcttga ggaggtagag
     1921 ggcttcctcc ttggtatgcc acttcagctg gttggtgagc atgaagatac ccgagacgtg
     1981 atcgtggatt ccgccgatga tggggccgcc gaacctcggg gagaggatgt tctcctctac
     2041 cgcaacgaga attgcggcct ccgccctcgc ctcctcggtc tgggggatgt gcaggttcat
     2101 ctcgtcgcca tcgaagtcgg cgttgtatgg cgggcatact gcggggttca gccggaatgt
     2161 gcggccgtcc atcactttga cacgatgggc catgatgctc atcctgtgga gcgagggctg
     2221 ccggttgaag aggacaatat cgccgtccct gatctggcgc tccactgtcc atcccggctc
     2281 gagcatctcg gcaacggtct caaggttccc ctcagccagc cggatacgcc tcttgtccgg
     2341 gcgaatcacg tagttggcac cgcaggggtc ggagagtctg gcgcggaacg ggccgtacct
     2401 caccatctgt ctcagttcct cgatgttgaa gggatttgtc tggacaggga tggtcatctc
     2461 gtttgcgact gccaggggta ttcccacctc gatcacgctc aggttcggat cgggagagat
     2521 gaccgttcgg gccgagaagt tcaccctttt ccctgagagc gatccacgga aacgcccgtc
     2581 ttttcctttc agcctctgtg agagtgtctt taagggcctg ccactcctgt ggcgtgccgg
     2641 cgggcaccct gccacctcgt tgtcaaggta ggtggtgacg tggtactgca ggagctccca
     2701 gaggtcttcg atgatcagtt gcggcgcacc cgcgtcctgg ttctccttga aacgctggtt
     2761 gatcctgatg atgtccacga gcttgtgggt gaggtcgtcc tcg
//
LOCUS       DAOQVJ010000189         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_42596_length_2798_cov_4.419978, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000189 DAOQVJ010000000
VERSION     DAOQVJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42596_length_2798_cov_4.419978"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKL26_04850"
     CDS             complement(<1..706)
                     /locus_tag="PKL26_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNS83056.1"
                     /translation="MAAKPYPRMVTFGCGQVECVPAEEFGNKAANLATIASLGIKVPP
                     GFCLGVTLCEEYFESGQHLPPDLPALLATGISRLEKATGLSFGSSRRPLLVSVRSGAP
                     VSMPGVMETVLNIGLSRDTLRGLISLSGNPKFAWDSYRRLIQNFGQVVSRHPSALYQS
                     VLREEMEREGVADEIELDAHTLRDLAAEYERIFSQNSGGKFPASAMQQLEQATEAVIK
                     SWESPRAEAYRRLNLIR"
     gene            complement(722..2665)
                     /locus_tag="PKL26_04855"
     CDS             complement(722..2665)
                     /locus_tag="PKL26_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83057.1"
                     /translation="MPAKKEIIEELGEEGLILPTLVNNALAANDRIKYLFTLLQAAQS
                     RAENPHADFSSLRVEREAAGVETTVYDRVVAESESAGEGVVRIPHAAHLFGEVRSNLL
                     EMATPLLSRDDDDAHTIRERLQALEQRIPTGEGDLAEKAAIDAITEGQSGDGDSVHLL
                     VMDMHRALNRLQGDLASEVIDGAMTYLLDEGDQEIVRAFMSGVRRTAPLKFDHPGLGT
                     TATRNGPRLIIQNDIGMTDAHVLVINIEGLTATIIYTDIHMPRLQFFQGLFEGKGVRW
                     GDTLSKKGAQTFEKKIYHLSTGTYTASGNDDLARFLSFAGSRLVFLIDWNRARKRLRN
                     FLLTKDAVSVLKWAADNDVGHMGFLLLGGEKMIYDAMEMSSRVPMRYGEPLHQILGRE
                     KSIEYFQWVLRVSATGLLANKSRLLVQDEIKAELLRYFRSAHENLMEICEEHATLTIS
                     VATVVREALQHIQMGGDVSFIARSAQRAKKWESEADLAVTKVRTLSRRIENADFYSAL
                     IFTADDALDFLEEASFFVTLIPSVTFSRKINQSLVEMAELAERSAQEFLKALIASQYV
                     YKGYSRDDMQEFLQAIDHVLSLEHQSDEALRLAQKTILVESRDWKEMQVYGDLARTIE
                     ESTNSLMKAAFLLRDDIMERMNR"
BASE COUNT          499 a          876 c          818 g          605 t
ORIGIN      
        1 cccgtatgag gttgagtcgc cgatacgcct ctgcccgggg actttcccat gacttgatca
       61 ctgcctcggt cgcctgctcg agctgctgca tggcgctggc aggaaacttc ccgccactgt
      121 tctgtgaaaa tatccgttcg tactcggcgg caaggtcgcg gagggtgtgc gcatccagtt
      181 cgatctcgtc tgcgactccc tcccgctcca tctcctcgcg gagcacagac tggtagagcg
      241 cagaggggtg ccgggacacc acctgcccga agttctggat gagcctcctg taggagtccc
      301 atgcgaattt cgggttcccc gagagggaaa taaggccgcg cagcgtatcg cgcgaaagcc
      361 cgatgttcag gacagtctcc atcacccctg gcatggagac tggcgcccct gaacggaccg
      421 agaccaggag tggcctcctg ctgctgccaa acgagaggcc cgttgccttc tccaggcggg
      481 agatccctgt tgcaaggagg gcgggcaggt ctggcggaag atgctgacct gactcaaaat
      541 actcctcgca gagggtcacc cccaggcaga acccgggggg cacctttatt cccagggatg
      601 caatggtggc aaggtttgct gccttgttgc caaactcttc tgccgggacg cactccacct
      661 ggccgcaccc gaacgtgacc atgcggggat atggttttgc tgccatcacc tcttcctcac
      721 ctcatcggtt catcctctcc atgatgtcgt cgcggaggag aaatgccgcc ttcatcaggg
      781 agttcgtgga ctcctcgatg gtccgggcca ggtcgccata gacctgcatc tccttccagt
      841 cccgcgactc cacgaggatg gtcttctggg cgaggcggag ggcctcgtcc gactggtgct
      901 cgagggagag gacatggtcg atcgcctgga ggaactcctg catgtcatcc ctgctgtacc
      961 ccttgtagac gtactgcgag gcgatcagcg cttttaagaa ctcctgggca ctcctctccg
     1021 cgagttcggc catctccacg agcgactggt tgatcttcct cgagaacgtg acggacggga
     1081 tgagtgtcac aaagaaggag gcctcctcca ggaagtcaag cgcgtcatcc gcagtgaata
     1141 tgagggccga gtagaagtcg gcattctcga tcctccggga gagggtccgc accttggtga
     1201 ctgcgagatc cgcctcgctc tcccatttct tcgccctctg tgcgctcctc gcgatgaagg
     1261 agacgtctcc ccccatctgg atatgctgca gcgcctccct gacaaccgtt gccacgctga
     1321 tcgtaagagt cgcgtgctcc tcgcagatct ccatcaggtt ctcgtgcgcc gaccggaagt
     1381 agcggagcag ttctgccttg atctcgtcct ggaccaggag gcgcgacttg tttgcgagga
     1441 gaccggtcgc ggagacccgg agcacccact ggaaatactc gatactcttc tcgcgcccga
     1501 ggatctggtg gaggggttcc ccgtaccgca tggggacgcg ggatgacatc tccatcgcgt
     1561 cgtagatcat cttctccccg ccgagaagca aaaaccccat gtgcccgacg tcgttgtccg
     1621 cggcccattt caggaccgaa accgcgtcct tggtgaggag aaagttccgc agcctcttcc
     1681 gggcgcggtt ccagtcgatc aggaagacca ggcgcgaccc tgcgaaggag aggaacctcg
     1741 caaggtcatc attcccggac gcggtatagg ttcccgttga gaggtggtag atcttcttct
     1801 cgaaggtctg cgctcccttc ttcgagagcg tgtcgcccca cctgaccccc ttgccctcga
     1861 agagtccctg gaagaactgc agcctcggca tgtggatgtc ggtatagatg atggtcgcgg
     1921 tcaggccctc gatgttgatc accagcacgt gggcatcggt catcccgata tcgttctgga
     1981 tgatgagtct tgggccgttc ctcgtcgcgg tggtcccaag gccggggtga tcgaacttca
     2041 ggggtgcagt gcgccggacc cctgacatga aggcacggac gatctcctgg tccccctcgt
     2101 caaggaggta ggtcatggcc ccgtcgatca cctcgcttgc gagatcgccc tggagcctgt
     2161 tcaaggcacg gtgcatgtcc atcaccagga ggtggacgct gtcgccgtct ccgctctggc
     2221 cctcggtgat cgcgtcgatt gctgccttct ccgcgaggtc tccctcgcca gtaggaatgc
     2281 gctgttcaag ggcttgcagt cgttccctga tcgtatgagc atcgtcgtcg tcgcgggaca
     2341 ggaggggtgt cgccatctcc aggagatttg acctcacctc cccgaagaga tgcgcggcgt
     2401 gggggatgcg gacgacccct tctcctgctg attccgattc ggccaccacc ctgtcgtaga
     2461 cagtcgtctc tacccccgca gcctcccttt ccacccggag gctggaaaaa tctgcatgcg
     2521 ggttctcggc cctggactgg gctgcctgga gaagggtgaa gagatacttg atccggtcgt
     2581 ttgccgcaag ggcgttgttg accagggtgg gaaggatcag cccctcctca ccaagctctt
     2641 ctatgatctc cttctttgca ggcatcgttt cctccacact gcatcgtttc cacgacttgc
     2701 accacccggc cctttccatg tatttccgga tatcacgtta atgtcctttt cagcccgcgg
     2761 gaataaaggc cttttccaca tatcactatt gagaatcc
//
LOCUS       DAOQVJ010000190         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43101_length_2776_cov_3.933848, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000190 DAOQVJ010000000
VERSION     DAOQVJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43101_length_2776_cov_3.933848"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKL26_04860"
     CDS             <1..1065
                     /locus_tag="PKL26_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83058.1"
                     /translation="RPDTIENGQPMFVTIRDLPGNSSFSLLVQADCAVEPNSEFRFQM
                     SQFVMPFALKQSSITATLSGTSTNRLEVMKESTIVTVSGQSRDGQFSTTKHYDITPGT
                     YDYFRLSGTSLPTARSITAQLQVTGTTQGPKDSEISFVVEGLTAGTITIAALVDGTQV
                     LYKSIPIGVSESSSAGDGVPVATTAPPPEQWNTFTSADGLVSVTVSHAGPIGFLKVPP
                     VDVTWGMQAISGPYTLVPQDTSFLPPGRIEFRIPAGAPRDGLVVASLDRETWTILSST
                     IGNTSVTAPVTISGTYALLGPVPVLATTTTTAITPIATTVEETPPTPTPADTTKAGIG
                     GATLILAICAGCAVAMRKIQ"
     gene            complement(1127..1972)
                     /locus_tag="PKL26_04865"
     CDS             complement(1127..1972)
                     /locus_tag="PKL26_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS83059.1"
                     /translation="MDDLVIRTAREEEVPVFIEWARREGWNPGLHDGECHYAVDPAGW
                     FVAEADGEIVGTVAVTNYDSRFSFGGFFVIREDMRSKGVGWQLSSAAIRHAGDRNLGI
                     DGVYEMQEKYSSRMGFRFAYRNIRWRGIADGRRQAGLGSAQEVPFSALVAYDALHFPA
                     ERKRFLERWIRTPGSWSSALVEGGSLRGYGVIRRCFEGYKIGPLFADTPEIGECILED
                     LTGRDEVRGQLFFFDTPEPNRDAVAMAQEREMEEVFGTARMYTKEIPPLPVGRIFGVT
                     TFEMG"
     gene            complement(2110..>2776)
                     /locus_tag="PKL26_04870"
     CDS             complement(2110..>2776)
                     /locus_tag="PKL26_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNS83060.1"
                     /translation="AYDMNGTVRALPFQQGLIGQNRLDIRDAHGVTFIRALVGAASRG
                     GGHVYYVYPNPAAGFAEELKVSSVMPVDDEWFIGSGIYLSHVNATFGRQEKDALMQRV
                     HAARDFAQREGMDAALAAFNDLSGQWAPGGSYIFSYAMNGTTLALPHQPELIGTSRLG
                     FLDHYGVAIIDWEIEVARGGGGFVYVVYLNPDTGSDALKLCYVVPVDREWFVGSGVYG
                     AEV"
BASE COUNT          532 a          983 c          774 g          487 t
ORIGIN      
        1 aggcctgaca cgatcgaaaa cggccagccc atgttcgtga ccatccgcga cctccccggg
       61 aactcctcgt tctcgctcct cgtgcaggcc gactgcgccg ttgaacccaa ctccgagttc
      121 aggttccaga tgagccagtt cgtgatgccg tttgcgttga agcagagcag catcacggcg
      181 accctctcag ggacctccac gaaccgcctg gaggtgatga aggagtcgac catcgtcacg
      241 gtctcgggcc agtccaggga tggccaattc tccaccacca agcattacga catcactcca
      301 gggacctacg actacttccg gctctcgggg acctcccttc cgacagcaag gagtatcacc
      361 gcacagctcc aggtgaccgg gaccacgcag gggccaaagg actccgagat atcgttcgtg
      421 gtcgagggcc ttaccgccgg gaccatcacc attgcagccc tcgtcgacgg gacgcaggtc
      481 ctttacaaga gcatcccgat tggggtgtcg gaatcgtcct ctgcagggga cggcgtgcct
      541 gtggccacta ctgcaccccc gccggagcag tggaacacgt tcacctcagc cgacggcctg
      601 gtcagcgtca cggtctcgca cgcgggaccc atcgggtttc tgaaggtccc tcctgtggac
      661 gtgacttggg ggatgcaggc catctccggg ccttacaccc tggtgcccca ggatacctct
      721 ttcctccccc cgggaagaat cgagttccgc atcccggccg gggctccgag ggatgggctc
      781 gtcgtcgctt cccttgaccg cgaaacatgg accatccttt cctcgacgat tggaaacacc
      841 tccgtgacgg ctcccgtcac catcagcggc acctatgccc tgctggggcc ggtcccggtc
      901 ctggcaacca ccacgaccac tgcgatcaca cccattgcga ccactgttga ggagactccc
      961 cccaccccca cgcctgcaga caccacaaag gcaggtatcg ggggtgccac gcttatcctc
     1021 gcgatttgtg ccgggtgtgc agtcgcgatg cggaagatac aatagcgcat gggtccggaa
     1081 ggagcagtgc atctccaccg cagcggaggt cgcctgctcc ccgctctcac cccatctcga
     1141 acgtggtgac cccgaagatc cggccgaccg gaagcggagg gatctccttc gtgtacatcc
     1201 tcgcggtgcc gaagacctcc tccatctccc gctcctgcgc cattgccacc gcgtcgcggt
     1261 tcggctccgg ggtgtcgaag aagaacagct gccccctcac ctcatccctc ccggtcaaat
     1321 cctccaggat acactcgccg atttccggcg tatcggcaaa gagcgggccg atcttatacc
     1381 cctcgaagca cctccggatt accccgtagc cccgcagtga tcctccctcc accaaggcgg
     1441 aggaccacga tccgggcgtg cggatccatc gctccaggaa gcgcttccgt tctgcgggga
     1501 aatggagggc atcatacgca acgagtgcgg aaaagggcac ttcctgcgca gagccgagcc
     1561 ctgcctgcct ccttccgtcc gcgatgcccc gccaccggat gttccggtac gcgaagcgga
     1621 accccatccg ggaagaatac ttttcctgca tctcgtagac cccgtcgatg cccaggttcc
     1681 ggtcgcccgc gtggcggatt gccgcgctcg agagctgcca gcccaccccc ttcgaccgca
     1741 tatcctccct gatgacaaaa aaacccccaa aagagaaccg ggagtcgtaa ttggtgaccg
     1801 caacagtccc gacgatctcg ccgtccgcct ccgcgacaaa ccacccggcc gggtccaccg
     1861 cgtagtggca ttccccgtca tgcagccccg ggttccaccc ctctctcctc gcccactcga
     1921 tgaagaccgg cacctcctcc tcccgcgccg tccggatcac aaggtcatcc atatgagagg
     1981 atgaagcggc aatggactta tagttcagtg ctgcggggag cagttgggcc atcggccccc
     2041 actgcgggga attccgtcgt gaaatgcccg tattacatat gatccacggg tcgaactgaa
     2101 cccacccact cacacctccg ccccgtacac tcccgacccc acgaaccatt cgcggtcaac
     2161 cggcaccacg taacagagct tgagggcatc ggacccggtg tcagggttca ggtacaccac
     2221 gtagacgaac cccccgccac cgcgtgccac ctcgatctcc cagtcgatga ttgcaacccc
     2281 gtaatggtcc agaaagccaa ggcggctggt cccgatcaac tcaggctggt gcgggagggc
     2341 gagggtcgtc ccgttcatcg catacgagaa aatgtagctt ccgccgggtg cccactgccc
     2401 ggagaggtcg ttgaacgcag cgagggccgc gtccattccc tccctttggg caaagtcacg
     2461 ggccgcatgc acccgctgca tcagggcgtc cttttcctgc cggccgaagg tggcgttcac
     2521 atgggagagg tagataccgg acccgatgaa ccactcatcg tcgaccggca tcaccgagga
     2581 gaccttcagt tcctctgcga accctgctgc ggggttgggg tatacatagt acacgtgccc
     2641 gcccccgcgg gaggctgccc ccaccagggc ccggatgaag gtaacgccgt gtgcgtcacg
     2701 gatatcaagg cggttctgcc ctatgaggcc ctgctggaac ggcagcgcac gcaccgtccc
     2761 gttcatgtcg taggcg
//
LOCUS       DAOQVJ010000191         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43131_length_2775_cov_3.341544, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000191 DAOQVJ010000000
VERSION     DAOQVJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43131_length_2775_cov_3.341544"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..647)
                     /locus_tag="PKL26_04875"
     CDS             complement(156..647)
                     /locus_tag="PKL26_04875"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNS83061.1"
                     /translation="MATQKSDPLSGQTAPPFCLPDSQGDEVCLPQYRGRWVILYFYPR
                     DNTPGCTLEALSFNNALESFAEMGAQVFGVSTDTPESHQRFADRHNLSILLLSDTSHR
                     VIEAYHSWKPKRIFGKEVLGTQRDTFLIDPEGRVVAVWRKVSPKGHVEEVREALVDRL
                     GRS"
     gene            complement(692..1060)
                     /locus_tag="PKL26_04880"
     CDS             complement(692..1060)
                     /locus_tag="PKL26_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS83062.1"
                     /translation="MVTIVHFDIPADIPDRARNFYAALFGWKFTSPPGYPDFSLIETT
                     GLEGHPGVGGGLGRRGEPGQDITVYFGVDSMERYLVLVKNEGGKVLMPYTPVPGYGAL
                     AICTDTEGNRFGLWESAEGS"
     gene            complement(1087..1215)
                     /locus_tag="PKL26_04885"
     CDS             complement(1087..1215)
                     /locus_tag="PKL26_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83063.1"
                     /translation="MYRHPLDEVGLVKTLATVRITTFLLAALPMTPNGNVYPYGTQ"
     gene            1393..>2775
                     /locus_tag="PKL26_04890"
     CDS             1393..>2775
                     /locus_tag="PKL26_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNS83064.1"
                     /translation="MVLPVTRRIPLPEADPYTLFRGLGAETGCILESMEGVPRRAVRS
                     IVGLEPSLVFTLDERITCKGDQRFSNMDICPTGSDPVEQLRILCNRFRCEGGRVTGFA
                     GGLVGYCAYDMVTDLSAGLVPAGKEGPLARFMLCTRGIVYDHVHGSCLLFDDPLLIEG
                     EDTGEAYDRANERMDLLEEAVMAVPGADPGEQGPGGGMELPLRTTMDREEFMAAVRKA
                     KEHIRSGDIFQVVLSRRLSCPYTGDPVLLYGAIRTINPSPYLYCIRFGDETVIGSSPE
                     MLVKVEGKAVQTVPIAGTRPRGSDLREDDQLAADLLADPKERAEHLMLVDLARNDIGR
                     VSAFGSVQVPEFMEVERFSHVQHLVSRVCGTLRDGCDRFDALAACFPAGTVSGAPKIR
                     AMQIIADLEAHPRGLYAGAVGYIGFNDALEFAIAIRTLVAREGVVEFSTGAGIVADSV
                     PEREFEETGYK"
BASE COUNT          563 a          783 c          842 g          587 t
ORIGIN      
        1 acacgcttga caggaatgca acacttctgg atcagatctc ctggctctcg gaggcagggt
       61 tctcgggcgt ggatattatc tacaagaacc ggactttttg cgtattcgtg ggtaagaagg
      121 aagaataggg cgggggaggg gccctgcacc ctgtttcagg aacggccgag cctgtcgaca
      181 agggcttccc tgacctcttc cacgtgccct ttcggcgaga ctttcctcca caccgccacc
      241 acccttccct cggggtcgat caggaaggta tctctctggg ttccaaggac ttcctttcca
      301 aaaatcctct tcggcttcca tgagtggtag gcttcgatga cccggtgact ggtgtcagag
      361 aggagaagga tcgagaggtt gtgccggtca gcaaaccgct ggtggctctc cggggtatcg
      421 gtgctcacgc cgaagacctg cgctcccatc tccgcgaatg actcaagtgc attgttgaag
      481 gagagtgcct cgagtgtgca cccgggagtg ttgtctcggg gatagaaata caggattacc
      541 catcttccgc ggtactgtgg aaggcacact tcatctccct gcgagtccgg caggcagaaa
      601 ggcggggcag tctggccgga taatggatcg gatttctgtg tcgccatatg acagaagaag
      661 ggaagccctg gcagggcttg aacgtattgg atcaggaccc ttccgcactc tcccagaggc
      721 cgaacctgtt tccctcggtg tctgtgcaga tggcgagcgc gccatatcca gggactgggg
      781 tatacggcat caggactttt ccgccctcat tcttgacaag gacgaggtac ctctccattg
      841 agtccacccc gaagtacacg gtgatatcct gtcccggttc tcctctcctt ccgagtcccc
      901 cgccaacacc tggatgacct tccaaacctg tggtctcgat cagggaaaag tcgggatagc
      961 ccggtggact ggtgaacttc catccgaaga gtgcagcata aaaattcctt gctctgtctg
     1021 ggatatccgc agggatatcg aaatgcacga ttgtgaccat gctctcttct ccttcgtggt
     1081 atggcgttat tgcgtgccat agggatagac atttccgttc ggtgtcatcg gcaatgccgc
     1141 caggaggaat gtggtgatcc ggacagtcgc gagggtcttt accagaccga cctcgtcaag
     1201 gggatgccga tacacccccc aaaaattgac accaatgtat atatataacc aataatgtac
     1261 ataaataaac atgataaaag agaacgaaca acccctggtg atgccgccgg aatggggatt
     1321 gccgccggcg cggcggcatg ttcagatcgt cctaaaggac tgatacctcc agggcgaaat
     1381 accgggccca tcgtggtcct cccagtcaca cgtcgcatcc cgctccccga agccgatcct
     1441 tacacgctat ttcgtggtct tggggcagaa acagggtgca tactcgaatc gatggagggc
     1501 gttcccagga gggctgtccg gtccatcgtg ggcctcgagc cgtcacttgt cttcactctc
     1561 gatgaaagga ttacctgtaa aggggaccag aggttctcca atatggatat ctgccccacg
     1621 gggtcggacc ctgtcgagca gttgcgtata ctctgcaacc ggttccgctg cgaggggggg
     1681 agagtcaccg gctttgcagg aggcctcgtg ggatactgcg cgtacgacat ggtgaccgat
     1741 ctctctgcag gccttgtccc tgcaggaaag gaaggcccgc tcgcgcggtt catgctctgc
     1801 acccgcggga tcgtgtacga ccatgtgcat ggctcctgtc ttttatttga cgaccccctg
     1861 ctcatcgagg gggaagacac cggggaggcg tatgacaggg cgaacgagcg gatggacttg
     1921 ctcgaggagg ccgtcatggc agttcccggg gctgacccag gagaacaggg gcctgggggt
     1981 ggaatggaac ttccccttcg cacgaccatg gaccgtgaag agttcatggc cgcggtcagg
     2041 aaagcaaagg agcacatcag gtctggggac atcttccagg tagtcctctc ccgccggctc
     2101 tcctgcccgt ataccggcga tccagtcctc ctctatgggg cgattcggac catcaacccg
     2161 tccccgtacc tttattgcat caggttcggg gacgagacgg taatcgggtc gagccccgag
     2221 atgctcgtga aggtcgaggg gaaagccgtc cagacggtcc ccatcgcggg gacaaggccc
     2281 cgtgggagcg atcttcgcga ggatgaccag cttgcagccg acctccttgc agatccaaag
     2341 gaacgggcgg agcacctcat gcttgtcgac ctggcccgaa acgacatcgg gagggtgtcg
     2401 gcatttgggt ctgtgcaggt ccccgagttc atggaggtcg agcgtttctc gcatgtccag
     2461 cacctcgttt ccagggtgtg cggaaccctc cgtgacggct gcgaccgctt cgacgcgctc
     2521 gcagcctgtt tcccggccgg gaccgtcagt ggtgcgccaa agatccgtgc aatgcagatc
     2581 atcgcagatc tcgaagcaca cccgcggggg ctctatgcag gggcggtcgg gtatatcggt
     2641 ttcaatgatg cccttgagtt tgccatcgcc atccgcacgc tcgtcgcgag ggaaggggtg
     2701 gtggagttct cgacgggcgc cgggattgtg gcggattctg tcccggaacg tgagttcgag
     2761 gagaccgggt acaag
//
LOCUS       DAOQVJ010000192         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43198_length_2772_cov_3.488406, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000192 DAOQVJ010000000
VERSION     DAOQVJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43198_length_2772_cov_3.488406"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PKL26_04895"
     CDS             <1..543
                     /locus_tag="PKL26_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNS83065.1"
                     /translation="YIATKGVWFFAVVSILLSFLVYEALQVNPMMAFGAVVGSTAFFI
                     VHGFRQNAEEQEKKMMGGNMSDISKIFYLEVIDATFSVDGVLGAFAFTLSVPLIIIGN
                     GVGAFVVRELTVRNVENIKKYRYLKNGAMYSIFVLGTIMILDSFGAHIPNWLSPVVTF
                     GIVGFFFYKSQRQIALQSRQ"
     gene            complement(627..1217)
                     /locus_tag="PKL26_04900"
     CDS             complement(627..1217)
                     /locus_tag="PKL26_04900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNS83066.1"
                     /translation="MYQNTNSHLLDPALLIIDMQNDFVRPGTPLQVTGVSSIIPKVRD
                     LLSLFREKDLPVFHVLRVHRASGVDVEITRREIFAKTPFAVEGTAGAAVISELTPLNG
                     EHIVKKTRMSAFFQTDLDIMLRTLGVDTLFIAGIQTPNCVRTTVFDAMAYNYRVFLVR
                     DAVGAKTDAIHEANVLDMQNIGIGIVECEGVPRLLP"
     gene            complement(1369..2199)
                     /gene="ablB"
                     /locus_tag="PKL26_04905"
     CDS             complement(1369..2199)
                     /gene="ablB"
                     /locus_tag="PKL26_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HNS83067.1"
                     /translation="MSDTICRYGSSTYQHGPLNNRVYLMRVTSKEIPEIFDIIEELAL
                     TSKYAKIVAKVPVSLSGDFLQRKYQKEALIPRFFHGKEDCVFMSRFLSYRNGKIPIPD
                     NSMYAIRGRGPVVNRTPVLPEGYRSQPCKESDVHALSALFSRVYETYPFPINNPDFLL
                     AMMREGTRYHGIWFGDTIVAASSAEVDANEMNAEMTDFATLPAHRGLGLGRYLLSSME
                     QDLEARDIITLYTIARALSRQVNRLFFGAGYSYGGFLFRNTQICGSCESMNVWYRHLI
                     "
     gene            complement(2192..>2772)
                     /locus_tag="PKL26_04910"
     CDS             complement(2192..>2772)
                     /locus_tag="PKL26_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNS83068.1"
                     /translation="REVTPSSCRALGKIADAGIPLGNQSVLLAGVNDCPRIIRALVHH
                     LVKNRVRPYYLYQCDLSEGLSHFRTPVAKGIEIMENLIGHTSGFAVPTYVIDAPGGGG
                     KIPMMPNYTVSSGVNRVVLRNYEGVICTYPEPDTYQRRTCDGDCVHCMLDHENPEGIE
                     ARPVGIAKLLADYDNASTLVPADTPRMERRNE"
BASE COUNT          617 a          724 c          751 g          680 t
ORIGIN      
        1 tacatcgcca caaagggggt atggttcttt gcggtggtct ccatcctcct ctcgttcctg
       61 gtttatgagg cgctccaggt gaaccccatg atggcgtttg gtgcggtggt cggctcgact
      121 gccttcttca tcgtccacgg ctttcgccag aacgccgagg aacaggagaa gaagatgatg
      181 ggggggaaca tgtcggatat atccaagatc ttctacctcg aggtgatcga tgcgacattc
      241 tcggtcgacg gggttcttgg ggcgtttgcg ttcacgctgt cggtgccgct tatcatcatc
      301 gggaacggtg ttggagcatt cgtcgtgagg gagctgacgg tccggaacgt cgagaacatc
      361 aagaagtacc gttacctcaa gaacggcgcc atgtactcaa tctttgtcct tgggaccatc
      421 atgatcctgg acagtttcgg tgcacatatt ccgaactggc tctcccccgt ggtcaccttc
      481 gggatcgtcg ggttcttctt ctacaaatcc caaagacaga tcgctctcca gtcacgccag
      541 taaggttaca tggtgaaccg catggagcaa ccggaaggaa gattccggga gaaagccaac
      601 ccctcgtaca gctcctttcc tggtcatcag ggcaacaagc ggggcactcc ctcacattcc
      661 acgatgccta tcccgatgtt ctgcatgtca aggacgtttg cctcgtgaat tgcatcggtc
      721 tttgcgccga ctgcgtccct taccaggaag acccggtaat tgtatgccat tgcgtcgaat
      781 accgtggtcc tgacgcagtt cggggtctgg atccccgcga taaagagcgt gtccaccccg
      841 agtgtcctca gcatgatgtc gaggtccgtc tggaagaaag cgctcatccg ggtctttttc
      901 acaatatgct ccccgttcag tggggtcagt tccgatatta ccgcggcccc ggcagttccc
      961 tctactgcaa agggggtctt cgcaaagatc tcccttcgcg taatctccac gtctacaccg
     1021 gatgcccggt gaactcggag gacatggaac accggcagat ccttctccct gaagagcgag
     1081 aggaggtccc ttaccttagg gatgattgaa gacactcccg tgacctggag aggtgtcccg
     1141 ggccggacaa agtcgttttg catgtcgatg atcaggaggg ctggatcgag aagatggcta
     1201 ttggtattct gatacatgga attgtctcga cattctgacc cgataaatcc atatcatgat
     1261 tcgaaggggt ttgatgcatg tattgttccc acggtggagc tggtaaattc agggtgtcca
     1321 ggggagggat cgccgctggg gatggaatgg tgccacaggg aactggggtt atatgagatg
     1381 tctgtaccag acgttcatac tttcacagga gccgcagatc tgggtgtttc gaaagagaaa
     1441 tcctccgtaa ctgtatccgg ccccgaaaaa aagccggttc acctggcgcg aaagtgccct
     1501 tgctatggta tagagggtga tgatatcccg ggcctcgaga tcctgttcca tcgatgaaag
     1561 gaggtacctt cccagtccca gtcccctgtg cgcaggaaga gtggcaaaat cggtcatttc
     1621 cgcattcatc tcgttggcgt ccacctctgc cgaagatgcg gcaacaattg tgtcaccaaa
     1681 ccagatccca tgataccgtg ttccctctct catcatcgcc aaaaggaaat cggggttgtt
     1741 tattgggaat ggatacgttt cgtacactct agaaaagagg gctgaaagtg catggacatc
     1801 cgactctttg cagggctgac tgcgatagcc ttcagggagg accggggttc tgttgacaac
     1861 aggacctctt cctcgaatgg catacatgga gttatcgggt ataggtattt tcccattcct
     1921 gtacgaaagg aacctggaca tgaagacaca gtcctccttg ccatgaaaaa atcgcggtat
     1981 taatgcctct ttttggtatt tcctctgtaa aaagtctcct gaaagtgaaa cagggacctt
     2041 cgcgactatc ttggcatact tggatgtaag ggcaagttct tcaataatgt cgaagatctc
     2101 tgggatctct tttgaggtca ctctcatcag gtatacgcga ttgttcagcg gcccatgctg
     2161 gtaggtagac gatccatacc ggcagatcgt gtcactcatt cctcctctcc attcttgggg
     2221 tatctgctgg aaccagcgtc gaagcattgt cataatcagc aaggagtttt gcaattccca
     2281 caggtcgtgc ctcaattcct tccgggttct cgtggtccag catgcaatgc acgcagtctc
     2341 catcgcaggt ccggcgctgg taggtatcag gttcgggata tgtgcagatc accccttcat
     2401 aattgcgcag caccaccctg tttactcctg atgagaccgt atagttgggc atcattggga
     2461 tcttgcctcc accacctgga gcatcgatga catacgtggg aaccgcgaat ccgctggtat
     2521 gaccgataag gttctccata atctcgatgc cttttgcgac aggggtccgg aaatgggaga
     2581 gcccctctga aaggtcgcac tggtagaggt aataggggcg caccctgttt ttcaccaggt
     2641 gatgcacgag agccctgatg atcctggggc agtcattcac ccctgccagc agaacggact
     2701 ggtttccaag agggataccc gcatccgcaa tcttaccaag tgcacggcag gaagacggag
     2761 tcacttcacg ag
//
LOCUS       DAOQVJ010000193         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43213_length_2771_cov_5.518409, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000193 DAOQVJ010000000
VERSION     DAOQVJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43213_length_2771_cov_5.518409"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKL26_04915"
     CDS             complement(<1..980)
                     /locus_tag="PKL26_04915"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNS83069.1"
                     /translation="MHMMQFGSTEPDPWTLDAHCDTWYMREFLRGPGLPPELQRVDTD
                     TFFQVTLPRLKEGNVRCLFLNTGDIGLLTSSAIIDSVHLWAGEESGKVSICHDAKEVH
                     HTVSSGKLAVVLACESSFLFQGRLDLLRNWYRLGVRVANITHGEGQEGMGWFGETALS
                     KDTQVRAASTLALQGTPSRDEYMAPESRDHLRYKEKGLTDFGRRAIDEMMHLGIVCDL
                     SHANDATFWDALSIREESGRGSFCCTHSNCAAICPHTRNLTDEMMEALADAGGVMGLC
                     FFGEFIDEHAPALGRYVDHLLHALDVMGQDHVGIGSDYDGVPPDAFMAIP"
     gene            complement(1065..1268)
                     /locus_tag="PKL26_04920"
     CDS             complement(1065..1268)
                     /locus_tag="PKL26_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HNS83070.1"
                     /translation="MADLPIAAVVRIAKQSGAERVGSDGAAALVAKAEDYIAGLVKEA
                     NKFALHAGRKTIKEEDIALAAKS"
     gene            complement(1500..1715)
                     /locus_tag="PKL26_04925"
     CDS             complement(1500..1715)
                     /locus_tag="PKL26_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83071.1"
                     /translation="MGTKIGKEKIKREAGYLYYLGKDGFVWAAPMKNNKTGKKKKVGT
                     EKIAKEKGYFYYLGKDGFVGKAKMKNA"
     gene            complement(1999..>2771)
                     /locus_tag="PKL26_04930"
     CDS             complement(1999..>2771)
                     /locus_tag="PKL26_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS83072.1"
                     /translation="IAGYLIEAFGVMNTFRIFGVMTIFLVFLLAQPLKFPDTGWIPLG
                     WNPDGREPNVAGHCECTRSRMLQTPSFYGLFACYFIGCLGGLMAISITKPIGTEVGVE
                     AGLATFLVGFFSIFNGFGRPFFGILTDLVTPRNATMVSFILIAAASILMWQIPTVPVY
                     VIAFALLWGCLGGWLAIAPTTTGNYFGTCDYPRCYGIVFLAYGAGAVAGPQLAAFIKT
                     STGTYAGVFPYLLALSLVGLVVAFILLKPPAEGPCKRD"
BASE COUNT          635 a          784 c          691 g          661 t
ORIGIN      
        1 tggggtatgg ccatgaatgc atcgggcggc accccgtcat agtcgcttcc gattcccaca
       61 tggtcctgcc ccatgacatc gagggcgtgc agcaggtggt cgacataccg cccgagtgct
      121 ggagcatgct cgtcaatgaa ctccccaaag aaacacaggc ccatcacccc tcctgcatca
      181 gccagcgcct ccatcatttc atcggtgagg ttccgggtat gggggcagat ggcggcacag
      241 ttgctgtgcg tgcagcagaa cgaccctctg ccactctctt ctcttatcga aagggcatcc
      301 cagaatgtgg cgtcgtttgc atgggagagg tcgcagacta tcccaagatg catcatctca
      361 tcgattgccc tccttccaaa atcggtcaat cccttctcct tataccggag atgatctctt
      421 gactccggag ccatgtactc gtcccggctc ggggtgccct gcagggcgag ggtacttgca
      481 gcccggacct gggtgtcctt tgagagggct gtttcgccaa accatcccat cccctcctgc
      541 ccctctccgt gggtgatatt tgccacccgc acccccaggc ggtaccagtt ccgaaggaga
      601 tccaggcgcc cctggaagag aaacgaactc tcacatgcca gcacgaccgc gagtttgccg
      661 gaactgaccg tgtggtgcac ctctttcgca tcatggcaga tcgaaacctt gccactctct
      721 tctcctgccc acaggtgcac gctgtctatg atcgcggagc tggtgagcag cccgatatcc
      781 cccgtattca ggaacaggca cctgacattg ccttccttta gccgcggaag tgtgacctgg
      841 aagaaggtat ccgtatccac cctctggagt tcgggcggga ggcccggacc ccggagaaat
      901 tcccgcatat accatgtgtc gcaatgtgcg tcgagtgtcc agggatccgg ttcagtgctc
      961 ccgaattgca tcatgtgcat gggtacaggc gcgatgtgat atggtttgaa aggatcagaa
     1021 gaaaacaaca ggagaaacga agagggttgt gggggagagg atgatcatga ctttgccgcg
     1081 agggcgatat cctcttcctt gatagtcttt cgtcccgcat gcagtgcgaa cttgtttgcc
     1141 tctttgacga gtcctgcaat gtagtcttct gcctttgcca cgagcgcagc cgcaccgtca
     1201 ctgccgacgc gctctgctcc gctctgtttt gcaatcctta caactgctgc aataggtaaa
     1261 tctgccacct tgattccctc aaagcaataa tgctttaaat agtatttata tattcccctc
     1321 ggatcttcac caaaaagcat cataaaatca aggattgcgg attgaaaggc cataaaaaag
     1381 ggtgtctcca tggactaccg catctgctcc cgcaacaaat ttttttcccg gttgtcgggg
     1441 gttacctgca gtcctcctgc aggaaatacc ccgtcagaat gaaaaatgga gtgtgtaatt
     1501 taggcattct tcatcttggc cttgccgaca aacccatcct tgccaagata atagaaatag
     1561 cctttctctt ttgcgatctt ctcagtaccg accttcttct tctttccggt cttattgttc
     1621 ttcatcggtg ctgcccatac aaagccgtcc ttcccaaggt aatagaggta tccggcttca
     1681 cgcttgatct tctcttttcc aatttttgta cccataatta atcttgcgat attgtcattt
     1741 ataagtgttt cgggtctccg ccctcgtttt ccctccttgc gggtatcacc acacggcctc
     1801 catatccctc attgctacct ctaagttaag tgagggcctg ctccttgcct cgctctattt
     1861 ccgattccat cccctggctt atttgcaccc tctgctccat tttctgggga aaaacaggtc
     1921 ttattagggt ttcccgagtg agggtatggg agggagattg tccattctga tcttcgcaat
     1981 ctgtgaaaaa taatgatgtt aatccctttt acaggggcct tctgcggggg gtttcagcag
     2041 gataaacgca accactaaac caacgagcga aagcgcaaga agataaggaa acactcctgc
     2101 ataagtccct gtggacgtct tgatgaacgc agccagttgc gggcccgcaa cagccccggc
     2161 gccatacgcg aggaagacta ttccgtagca cctcggatag tcgcaggtgc cgaaataatt
     2221 cccggtggta gtcggtgcga tggccagcca accccccaga cacccccata acagcgcaaa
     2281 ggcaatgaca tagaccggaa ccgtcgggat ctgccacatc agaatggagg ctgctgcaat
     2341 caggataaac gataccattg tcgcgttccg cggggtaacc agatcggtga gtatcccgaa
     2401 gaagggacgc ccgaacccgt taaagatcga gaaaaatccc acgagaaacg tggcaagccc
     2461 tgcttcaact cctacttcgg tgccaatcgg tttggtgatc gagattgcca tgaggcctcc
     2521 gaggcagcca atgaaatagc aagcaaagag tccatagaaa gagggggttt ggagcatcct
     2581 ggaccgcgtg cattcacaat ggccagccac gttgggttct ctgccgtccg ggttccatcc
     2641 cagggggatc cagccggtat ctggaaattt caggggttgt gcaagaagga ataccaggaa
     2701 gatagtcata acgccaaaga tcctgaacgt gttcatgacc ccaaaggcct cgatcaggta
     2761 ccctgcaata t
//
LOCUS       DAOQVJ010000194         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43277_length_2768_cov_4.588279, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000194 DAOQVJ010000000
VERSION     DAOQVJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43277_length_2768_cov_4.588279"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="argS"
                     /locus_tag="PKL26_04935"
     CDS             complement(<1..974)
                     /gene="argS"
                     /locus_tag="PKL26_04935"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNS83073.1"
                     /translation="MLSTLYHTIEHALKEATGQEEVFLVEGGEHADLASTVAFGLAKV
                     RRKNPAEISRELAETLAAREDFGDIAVEALGPYLNFHFSREVIERALQDATRPGYGTH
                     PRREERVVLEHTSANPNGPLHVGHIRNTILGDTLGRCFRKAGYPLEVQYYVNDMGRQI
                     AIVVWGFCTIADTSLAGEKGDHHVARIYIAANRALEARPECVAEVDRLMQQVERGDQA
                     TVLLFRKAVTECLEGFRVTLQRLGARHDRFVWESDFIRNGDTDRVLSRISHMPECHDD
                     GKLWLDLSESGFEKEYVLRRSDGTSVYAARDLAYHTWKGRNFDRVIDVL"
     gene            complement(978..2252)
                     /gene="prf1"
                     /locus_tag="PKL26_04940"
     CDS             complement(978..2252)
                     /gene="prf1"
                     /locus_tag="PKL26_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HNS83074.1"
                     /translation="MTGTPEMDEGRRRYEFKKTLEKLMAKQGSGTELISLYIPPDKQI
                     HDVTAQLRDEFGQCANIKSKQTRTNVQSAISSILSRLKYYKNPPPHGMAVFCGTIQLQ
                     GDRTDLECTIIEPPEPLNLYMYRCSSAFELDPLKQMLEEKNVYGLLVLDRREAYWGFL
                     RGNRIEPIGGVTSTVPGKQRKGGQSSVRFQRLREIAINEFYTKIGERSSDIFLAEKDF
                     FERFKGVLIGGPSPTKEEFEAGQYLHHEIQKRITGLFDVAYTNESGLAELVDAAKDAL
                     KGMEVIKEKEFMNRFLKELVKENGLAAYGEESVRKNLDVGSVDILLLSGSLRKSRVSI
                     RCQACGHADERTIQLEPGKTVEDILASQTCRKCNGPLVVDQEVDIIEELTRLADQTST
                     RVEIISDDFEEGSILYTAFGGIAAILRYRTGF"
     gene            complement(2290..>2768)
                     /locus_tag="PKL26_04945"
     CDS             complement(2290..>2768)
                     /locus_tag="PKL26_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS83075.1"
                     /translation="DYQRFLAHEWPQCYLSICDIHCTRAFLKEKTHVHVLRPGGEQRG
                     RYSAIAAALDLEFAGFIGVPTSGFSRAGESYSGDLRRSLLSIDPDACAERMAPWSNWA
                     LHSPGDTVIPFADGQALYQVLPEPKSFFPFNGTHGLNEEADAWIPGECAQIYGPAR"
BASE COUNT          506 a          852 c          807 g          603 t
ORIGIN      
        1 aggacatcga tcacgcgatc gaagttgcgg cccttccatg tgtggtaggc aaggtcccgg
       61 gcagcataga cggaggtccc gtcgctcctc cggagcacgt attctttctc gaagccggat
      121 tccgagagat cgagccagag cttgccatca tcatggcact ctggcatgtg cgatatcctg
      181 gacagcacac ggtcggtatc accgttgcgg atgaagtcgc tctcccacac gaaacggtcg
      241 tgcctggcgc ccagccgctg aagggtcacc ctgaaccctt caaggcactc cgtgacagcc
      301 ttcctgaaga ggaggacggt cgcctggtca cctctctcga cctgctgcat caggcggtcc
      361 acctcggcga cgcattcggg cctcgcctcg agggccctgt tggccgcgat atagatcctg
      421 gcaacatggt ggtcgccctt ctcaccggcc agggaagtgt ccgcaatggt gcaaaagccc
      481 cacaccacga tggcgatctg gcggcccatg tcatttacat agtactggac ttcaagagga
      541 taccccgcct tccggaaaca cctgcccagc gtgtctccga ggatcgtgtt cctgatgtgc
      601 ccgacgtgaa gggggccgtt tgggttggcg ctggtgtgct cgaggaccac acgctcctcc
      661 ctgcgcgggt gtgtaccgta gccgggacgc gtggcatcct ggagtgcccg ctcgatgacc
      721 tcccggctga aatggaaatt caggtagggt ccaagggcct cgaccgcgat atccccaaag
      781 tcctccctcg ctgcaagtgt ctctgccagt tcccgggaga tctcggccgg gtttttccgc
      841 ctcaccttcg cgagcccgaa tgcaacggtg cttgcgagat ccgcgtgttc tcccccctcg
      901 accaggaaca cctcctcctg gccggtcgcc tctttgaggg catgctcgat ggtatggtag
      961 agtgttgaca gcatggttca gaaccccgtc cggtaccgga ggatggccgc gattcccccg
     1021 aaggcagtat aaaggatcga cccctcctcg aaatcatcgg agatgatctc gaccctcgtg
     1081 ctggtctggt cggcgagcct ggtcagttcc tcgatgatat cgacctcctg gtcaacaacc
     1141 aggggcccgt tgcacttccg gcatgtctgg ctggccagga tatcctcgac ggtcttcccc
     1201 ggctcgagct ggatggtccg ttcgtcagcg tgcccgcatg cctggcacct gatgctgacc
     1261 ctggacttcc ggagggagcc ggagaggaga aggatatcca cagagccgac atccaggttt
     1321 ttcctgacac tctcctcccc gtaggcagca aggccgttct ccttcacaag ctcttttaag
     1381 aaccggttca tgaactcctt ctccttgatc acctccatcc ccttcagggc gtcctttgcc
     1441 gcatccacga gttctgcaag gccgctctcg ttcgtatagg ccacgtcgaa gagtccggtg
     1501 atccgcttct ggatctcgtg atggaggtac tggccggcct cgaattcctc cttggtgggg
     1561 ctcggccccc cgatgaggac gcccttgaaa cgttcgaaga agtccttctc ggcaaggaag
     1621 atgtcgcttg accgctcacc gatcttcgtg tagaactcgt tgatggcgat ctcccttaaa
     1681 cgctggaaac ggacgctcga ctggcctccc ttcctctgct tgcccggaac agtcgaggtg
     1741 acacccccga tcggctcaat ccggtttccc cggaggaacc cccagtatgc ttctctccgg
     1801 tcaagcacga ggagcccgta gacgttcttc tcctcgagca tctgcttgag gggatcgagc
     1861 tcgaatgccg aactgcaccg gtacatgtag aggttcagtg gttcgggagg ttcgatgatg
     1921 gtgcattcca ggtcggtgcg gtcaccctgg agctggatgg tcccgcaaaa gaccgccatg
     1981 ccatggggcg gggggttctt gtaatatttg agccgcgaga ggatcgagga tatggcgctc
     2041 tggacgttgg tgcgcgtctg cttgctcttg atgttcgcgc actggccaaa ctcatcccgg
     2101 agctgcgcag tcacatcgtg gatctgcttg tccggaggta tgtagaggga tatgagctcg
     2161 gtgccgctcc cctgcttggc cataagcttc tcaagggtct tcttgaactc atagcgcctt
     2221 cggccctcgt ccatctccgg ggtcccggtc atctccctct ctgcggacat atcaaagctt
     2281 caacatgaat caacgcgcgg ggccataaat ttgcgcacat tccccaggta tccatgcatc
     2341 tgcctcttcg ttgagcccgt gggtgccgtt gaaggggaag aacgatttcg gctcgggcag
     2401 cacctggtat aatgcctgcc cgtcggcgaa ggggatgacc gtatccccgg gcgagtggag
     2461 ggcccagttg ctccatggtg ccatgcgttc tgcacaggca tcggggtcga tcgagagaag
     2521 ggatctccgg agatcgcccg agtacgattc accggctcgg gagaatcccg atgtcgggac
     2581 accgatgaac cctgcaaact ccaggtccag agctgcagca atggccgagt accgtccccg
     2641 ctgctctccc ccagggcgta atacgtgtac atgagtcttc tccttaagga atgcccgggt
     2701 gcaatggatg tcgcatattg agagatagca ttggggccac tcgtgggcaa ggaacctctg
     2761 gtaatcgc
//
LOCUS       DAOQVJ010000195         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43508_length_2758_cov_3.524232, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000195 DAOQVJ010000000
VERSION     DAOQVJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43508_length_2758_cov_3.524232"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKL26_04950"
     CDS             <1..365
                     /locus_tag="PKL26_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNS83076.1"
                     /translation="RIMLGTFALSSGYYGKYYAKAQAARNNVRADFERLFREVDVLAG
                     PTMPTVAFRFGEKSDPLSMYLSDILTVPANLAGVPAISVPCGKVERLPVGLQLMGRWM
                     EDERVVDVAYAFEQGGSA"
     gene            362..1801
                     /gene="gatB"
                     /locus_tag="PKL26_04955"
     CDS             362..1801
                     /gene="gatB"
                     /locus_tag="PKL26_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNS83077.1"
                     /translation="MTVIVGLEIHCQLDTATKLFCGCSTDYRDDLPNSHVCPVCLGLP
                     GSLPKVNRKAVEYALMVAKALNCEVPEESEFARKNYFYPDLDKGFQITQYDKPLAVWG
                     YLDIEGDEGEKRVRITRVHMEEDPGRLVHKGGADRPKYTLVDYNRAGIPLIEIVTEPD
                     LRSPKEARRFLNKLRATLEYLGVFDSEKEGSLRVDANISIKGSERVEVKNITSYKGVE
                     KALMFEVTRQKNAIRRGQRIVRETRHFVEARGVTTSSRSKEMEQDYRYFPEPDLRPLR
                     VCDWAEEIEIPELPDARKQRFIEDYRLSPTHAATLTGDWQIAEFYDSLPGDLKVLTAT
                     ATWVADTLLGELNYRDMGIRGVPLDHFKELLLIRQDGEITEKAAVEVLRSLLDQIRES
                     GTCELPRQCIGRLGLFVIAGGEGSGDDPVTAAAREAIAENAQAVADYRQGRKEALNFL
                     VGQVMRKTRGCAKPADVNRVLSNLLSGEE"
     gene            1810..>2758
                     /locus_tag="PKL26_04960"
     CDS             1810..>2758
                     /locus_tag="PKL26_04960"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNS83078.1"
                     /translation="MYLIVTEKNISARRISGILAGKERVREVKDGGVSSYLFDETVVV
                     GLKGHVVEVDFVEGYSNWRSESRTPRSLIDAPTVKVPTEKKIVSLIQKLARKATRVTI
                     ATDFDTEGELIGKEAYELVRDANPRVRVDRARFSAITPEEIRHAFSATTDLDFDLAAA
                     GEARQVIDLVWGASLTRFLSLAARRGGANILSVGRVQSPTLAMIVDREREIDSFVPEK
                     YWQLHLLTEKQGQQMEARHTTSRFTERGEAEGARDRTKEPLVVTEVKEGVKVDRAPSP
                     FDTTSFIVAAARLGFSAASAMRLAEDLYMNGFISYPRTDN"
BASE COUNT          568 a          851 c          901 g          438 t
ORIGIN      
        1 ggaggatcat gctcgggacc tttgcactct cttcgggata ctacgggaaa tactacgcca
       61 aggcccaggc ggcacgaaac aacgtccggg ctgacttcga acgcctcttc agggaagtgg
      121 acgtgctcgc cgggccgacc atgccgacgg tcgctttcag gttcggcgag aagagcgacc
      181 ccctctccat gtacctctcg gatatcctca ccgtgcccgc gaaccttgcc ggcgtccctg
      241 cgatctcggt cccctgcggg aaggtggaaa ggctccccgt cggcctgcag ctcatgggcc
      301 ggtggatgga ggacgagcgg gtagtggacg tcgcatacgc gttcgagcag gggggatcgg
      361 catgaccgtg atcgtaggcc tcgagatcca ctgccagctg gataccgcca ccaagctctt
      421 ctgcgggtgc tcgaccgatt accgggacga cttgccgaac tcgcacgtct gcccggtctg
      481 cctgggcctg cccggatcac tcccgaaggt aaacagaaag gcggtcgaat acgccctcat
      541 ggtcgcaaaa gccctgaact gcgaggtccc ggaggagtcg gagtttgcaa ggaagaacta
      601 cttctacccc gaccttgaca aggggttcca gatcacccag tacgacaagc ccctcgccgt
      661 ctgggggtac ctcgacatcg agggagacga gggcgagaag agggtgcgga tcacccgtgt
      721 ccacatggag gaggacccgg ggaggctcgt ccacaagggc ggtgcggacc ggccgaagta
      781 caccctcgtc gactacaacc gggccggtat ccctctgata gagatcgtga ccgagccgga
      841 cctccggtca ccgaaggagg cccggaggtt cctgaacaag ctcagggcga ccctcgagta
      901 cctcggcgta tttgacagcg agaaggaggg ctcgctccgt gtggatgcaa acatctccat
      961 aaaagggagc gagcgggtgg aggtcaagaa catcacctcc tacaagggcg tcgagaaggc
     1021 gctcatgttc gaggtgacac ggcagaaaaa cgccatccgg aggggacaga ggatcgtcag
     1081 ggagactcgc cacttcgtcg aggcacgggg cgtgaccact tcctcacgga gcaaggagat
     1141 ggagcaggac taccggtatt tcccggagcc cgacctccgc ccgctgcggg tatgcgactg
     1201 ggcagaggag atcgagatcc ccgagctccc cgatgcgagg aagcagaggt tcatcgagga
     1261 ctatcggctc tcacccaccc atgccgcgac cctcacaggg gactggcaga tcgccgagtt
     1321 ctacgacagc ctcccggggg acctgaaggt cttgactgcg accgcaacct gggtggccga
     1381 cacgcttctc ggggagctca actaccgcga catgggcatc cgcggtgttc cgctcgacca
     1441 cttcaaggag ctcctcctca tccgccagga cggcgagatc accgagaagg cggctgtcga
     1501 ggtcctgaga agcctccttg accagatcag ggagtcggga acatgcgaac tcccgcgcca
     1561 gtgcatcggg agacttgggc tcttcgtgat cgcgggcggg gagggaagcg gggacgatcc
     1621 cgtgaccgcg gccgcacgcg aggccattgc cgagaacgcc caggcagtcg ccgactatcg
     1681 gcagggcagg aaggaggccc tgaacttcct cgtcgggcag gtgatgagaa agacccgcgg
     1741 gtgcgcaaag cccgcagacg tgaaccgggt gctctcgaac ctcctctccg gggaggagtg
     1801 aggagaccca tgtacctcat cgtaaccgag aagaacatct ccgcacgcag gatctccggg
     1861 atcctggccg ggaaagagag ggtccgcgag gtaaaggatg gaggggtctc gagctatctg
     1921 ttcgacgaga ccgtggtcgt cgggctgaag ggccacgtcg tcgaggtgga cttcgtggag
     1981 ggatactcca actggcggag cgagtcccgc acgccgcgga gcctcatcga cgccccaacc
     2041 gtgaaggtcc cgaccgagaa gaagatcgtc tccctcatcc agaaactggc ccgaaaggcg
     2101 acgcgcgtca cgattgcgac cgactttgac accgaggggg agctgatcgg caaggaggcg
     2161 tacgaactcg tcagggacgc aaacccgcgt gtccgcgttg acagggcacg gttctctgcc
     2221 atcactcccg aagagatacg ccacgcgttc tctgcaacta cggatctcga cttcgatctc
     2281 gccgccgcag gtgaggcgag gcaggtgatc gacctcgtct ggggggcgtc actcacccgg
     2341 ttcctctccc ttgcggcacg gcggggtggg gccaatatcc tctctgtcgg gagggtccag
     2401 agcccgacgc ttgccatgat cgtggaccgc gagcgggaga tcgattcctt tgtccccgag
     2461 aagtactggc agctgcacct cctcaccgag aagcaggggc agcagatgga ggcacggcac
     2521 accacttccc ggttcacaga gaggggggag gccgaagggg cccgcgaccg gacaaaggag
     2581 ccgctcgtcg tcaccgaggt gaaagagggg gttaaggtcg accgggcccc atcgccgttc
     2641 gacactacct ccttcatcgt cgctgccgca cgcctcgggt tctctgcggc aagcgccatg
     2701 cgccttgcag aggaccttta catgaatggg ttcatctcct acccgaggac cgacaaca
//
LOCUS       DAOQVJ010000196         2755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_43578_length_2755_cov_4.515185, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000196 DAOQVJ010000000
VERSION     DAOQVJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43578_length_2755_cov_4.515185"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKL26_04965"
     CDS             <1..1093
                     /locus_tag="PKL26_04965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNS83079.1"
                     /translation="NLVEDLVINRERITEIAREHQMKNLDETLNMIGRSVSDLQNLMM
                     NIRMIPLNHIFNRFPRTVRDIANREGKEVEFIIEGGDTELDRSVMDGLNDPLLHLIRN
                     AIDHGIERPDVRETAGKPRKGTLLLSARRDRDNVIITIQDDGKGISAERVKRKAIERG
                     LVFEAEAENLSREEIIDFLFRPGFSTADAITDISGRGVGLDVVRTTIESLKGTIKVES
                     TEGAGSRFELLLPPTMAIVTVMMVRINGRRCAIPVHNVVEVASLERECIHSIGGHETI
                     LLRDEVLPLDRLDDMFGHSERTDILVVLQYQNRKRSIAVDLIEGQQEVVIKPLSTVVG
                     TCRGVSGVTIPGDGEVVPVLDVNALIKES"
     gene            1098..1703
                     /locus_tag="PKL26_04970"
     CDS             1098..1703
                     /locus_tag="PKL26_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNS83080.1"
                     /translation="MLNSHQRDALRELGNIGAAHAATTLSTMLMSNIEMEVPEISVVD
                     IAKIHEHVGDELSALVIFQITGEVSGGGYLLLHIPKNSVIRLTNAMLGMTDLERDLTE
                     MDESALLEIGNIMVSAFLDATATLLSIIMLPSPPSMIIDMPHAAFQTILAMQEFSEID
                     QVVIFRTELRSDQHKISSNLFLLPNKPMLDEILSMLENLMK"
     gene            1719..2201
                     /locus_tag="PKL26_04975"
     CDS             1719..2201
                     /locus_tag="PKL26_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNS83081.1"
                     /translation="MPGPDEQPVIVGIGEFHAGKNPMSSIGLGSCIGLVIFDREKPIG
                     ALAHIMLPDSQGRAERPAKYADTAVIHLVKELNKLGCRTPSLAAKICGGASMFQAFSG
                     NLNIGERNVEAVRVHLKKLSLSIVAEEVGGTVGRTIVYNPAENGKISVKTADGTRKII
                     "
     gene            complement(2198..2752)
                     /locus_tag="PKL26_04980"
     CDS             complement(2198..2752)
                     /locus_tag="PKL26_04980"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNS83082.1"
                     /translation="MVVPSRVFFTKGVGVHKDRLASFELALRKAGIEKFNLVYVSSIF
                     PPNCKMVSVEEGTKMLEPGEITYCVMAKNDTNEPNRLISAAIGLALPKEAREYGYLSE
                     HHAYGETAEKTGEYAEDLAATMLATTLGIEFDINQAWQEREQIYKASGKIIRTTQVCQ
                     SAQGDKNGMWTTVIAAAVFVTNRT"
BASE COUNT          646 a          779 c          784 g          546 t
ORIGIN      
        1 gaacctcgtc gaggaccttg tgatcaaccg cgaacgcatc accgagatcg cgagggaaca
       61 ccagatgaag aacctcgacg agaccctcaa catgatcggc cggtcggtct ccgatctcca
      121 gaacctgatg atgaacatca ggatgatccc tctcaaccac atcttcaaca ggttcccaag
      181 gacagtcagg gacatcgcca atcgggaagg aaaggaagtc gagttcatca tcgagggtgg
      241 ggacaccgag cttgaccgga gcgtcatgga cggcctcaac gatccactgc tccacctgat
      301 caggaatgcc atcgatcacg ggatcgagag gcccgatgtc cgtgaaacgg ctggaaagcc
      361 aaggaagggg acgctcctcc tttcggcccg gagagaccgc gacaacgtca tcatcaccat
      421 ccaggacgac gggaagggga tcagtgcaga aagagtgaag agaaaagcca tcgagagggg
      481 gcttgtcttt gaggcggagg ccgaaaacct ctcccgggaa gagatcatcg acttcttgtt
      541 ccgaccgggg ttctccaccg ctgacgcgat caccgacatc agcggaagag gggtcggcct
      601 cgacgtggtg cgcaccacga ttgagtccct gaagggaacg atcaaggtag agtcaacaga
      661 gggtgcaggc tcgaggttcg agctcctcct cccccccacc atggccatcg tcacggtgat
      721 gatggtccgg ataaatggcc ggaggtgcgc gatacccgtc cacaacgtgg tggaggttgc
      781 gagcctcgag agagagtgca tccattcgat cgggggacac gaaacgatcc ttctcaggga
      841 cgaggtactc cccctggacc gccttgacga catgttcggc cattcggaac ggaccgacat
      901 cctggtagtg ctccagtacc agaaccggaa gcggagcatt gccgtcgacc tcatcgaggg
      961 ccagcaggag gtagtgatca agccactctc cacggtggtg ggaacctgcc gcggggtatc
     1021 cggggttacc atcccgggtg acggcgaggt tgtgccggtg cttgatgtga atgcattaat
     1081 caaggagagt tgaaggtatg ctcaacagcc accaacgtga cgcattacgg gaactcggaa
     1141 acatcggcgc agcacacgcg gcgaccacac tttccaccat gctgatgtcg aatatcgaga
     1201 tggaagttcc cgagatcagc gtggtcgata tcgcaaagat tcatgaacac gtgggggatg
     1261 aactctcggc gcttgtgatc ttccagatca cgggagaggt ctcgggggga ggatacctcc
     1321 ttttgcatat tccaaagaac tcggtgatac ggctcaccaa cgccatgctc ggcatgaccg
     1381 atctcgagcg cgatctcacc gagatggacg agagcgcgct cctcgagatc gggaacatca
     1441 tggtatcggc gttccttgat gcgaccgcaa ccctcctctc gatcattatg ctgccttcac
     1501 cgccgtccat gatcatcgat atgccgcatg cggcgttcca gacaatcctt gccatgcagg
     1561 aattttctga gatcgaccag gtcgtcatct tccgaaccga gctccgcagc gaccagcaca
     1621 agatctcaag caacctcttt ttgctcccga acaagcccat gctcgacgaa atcctctcaa
     1681 tgctcgagaa cctgatgaaa taaagaacag gaaaaacgat gcctggccct gacgaacaac
     1741 cagtgattgt ggggattgga gagttccacg cagggaaaaa cccgatgtct tcgattgggc
     1801 ttgggtcctg catcgggctc gtcatattcg acagggagaa gcccatcggc gccctcgccc
     1861 atatcatgct gcctgattcg caggggcggg cggagcgacc cgcaaaatat gcagataccg
     1921 cagtcattca cctggtaaag gagttgaaca aactcgggtg caggacgccc tcgctggcag
     1981 cgaagatatg cggcggtgca tccatgttcc aggccttctc cggtaacctg aatatcggcg
     2041 agaggaacgt ggaggcggtc agggtgcatc tcaagaagct gagcctttcc atcgttgcag
     2101 aagaggtcgg cgggaccgtt gggaggacaa tcgtgtacaa tcccgcagaa aacggaaaaa
     2161 tcagcgtgaa aacggctgat gggacgcgaa aaataattta ggtgcggttg gtcacaaaga
     2221 ctgcggctgc aatgaccgtg gtccacatcc cgttcttgtc accctgggca gactggcaca
     2281 cctgggtggt cctgatgatc ttgccgctgg ccttgtagat ctgctcccgc tcctgccatg
     2341 cctggttgat atcgaattcg atgccgaggg tggtcgcaag catggtcgcg gcaaggtctt
     2401 ctgcgtactc tcctgtcttt tctgccgtct caccgtaggc atggtgttca gagagatacc
     2461 cgtattccct ggcctccttc gggagcgcaa gcccgattgc cgccgatatc agcctgtttg
     2521 gctcgtttgt atcgttcttg gccatcacgc agtaagtgat ctccccaggc tcaagcatct
     2581 tcgtgccctc ctccacagag accatcttac aattcggggg aaatatgctc gatacataga
     2641 ccaggttgaa cttctcgatt cccgccttcc gaagcgccag ctcaaaggag gcgagcctgt
     2701 ctttgtgaac acccaccccc tttgtaaaga ataccctgct cgggaccacc atatg
//
LOCUS       DAOQVJ010000197         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44075_length_2735_cov_3.182836, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000197 DAOQVJ010000000
VERSION     DAOQVJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44075_length_2735_cov_3.182836"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKL26_04985"
     CDS             complement(<1..389)
                     /locus_tag="PKL26_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83083.1"
                     /translation="MGGDAKIIEQLGLLLQRRSARLPGIQVDMAQAVIDSISHDIIFR
                     EMDAQKVEDPLAGAQPAISFKPVCVIALDVYILTVSFRVADDQHFATIREGFHMVRSF
                     DGAAQFIRTEQLLAGFGSLTIFRVIKA"
     gene            408..725
                     /locus_tag="PKL26_04990"
     CDS             408..725
                     /locus_tag="PKL26_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83084.1"
                     /translation="MEQLAEYVEQGHYMIAEVNAGVLFDEVQGIGDDPEQSNHAIVIT
                     GVARDLQTGSIVGFYVNDTGGFCRNSDGSARGGALVDAAHMKSGWADTGGLCVITEPR
                     RSG"
     gene            971..1273
                     /locus_tag="PKL26_04995"
     CDS             971..1273
                     /locus_tag="PKL26_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83085.1"
                     /translation="MLYALTSTIPFPSGAQPESVEAPPQARSVSDVIETVTRIERLMD
                     CLVRDFFSGERGDPEQKNALIGELKQHLPGPLLTPYQVLAPDFFAWLEESQPLPEE"
     gene            1277..2149
                     /locus_tag="PKL26_05000"
     CDS             1277..2149
                     /locus_tag="PKL26_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83086.1"
                     /translation="MNSHREQARTGAGIIPGTSFSLKGRWLERTKTLPDEQDGKPRDI
                     ALCAIRKSFEEFREEAKKEVRTGDAGTLMFRLLDQIRQFIRFYELAEEPAKSAPADTG
                     PVDRKDSRESGYQDQEQTGPSPHTGRRGINPMKLLGARTKPVEEAPASAPSFRREQLE
                     MLVGNIGDFLGVMDDMLPKLLPKPEQKPDLPRDSLEAFQGMLAIMLEADDRRFTEAMR
                     SQREVICSLFGKYGISTVLFEEEPDRNRKGEWFEERLVSPEKFPTIRTASPALVRNDM
                     LICRGTVYIPQRPR"
     gene            2188..>2735
                     /locus_tag="PKL26_05005"
     CDS             2188..>2735
                     /locus_tag="PKL26_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83087.1"
                     /translation="MSLDNDVQLAQTFDEIDKSVLSSFTHIIGFDLGHGEFSLFQVSD
                     EQNDSAKCIEINNKKSQITAIAVKDTGEILVGNKAITTYDEAVITVGFKRKPGENPNL
                     DHLVGLFFAGVLRSALAERKVSLSNKTLILVGCPSEWTREQQRGYQAILQEAISDLPC
                     ETKPKVAVVPESRAAIQCARQSG"
BASE COUNT          654 a          718 c          810 g          553 t
ORIGIN      
        1 tgggctttga taaccctgaa tatagtgaga ctcccgaacc ctgcaaggag ctgttccgtc
       61 ctgatgaact gggccgcacc atcgaagctc cttaccatgt ggaaaccttc ccggatggtc
      121 gcgaagtgct ggtcatcggc gacccggaac gagacggtca ggatatacac atccagggcg
      181 ataacacata cgggtttgaa ggagattgcg ggttgtgctc ctgccagggg atcctcaacc
      241 ttttgggcat ccatctctcg gaagatgata tcgtgcgata tgctgtcgat cacggcctgt
      301 gccatatcga cctggatccc aggcaatcgg gcggaacgac gctggagcaa cagaccgagc
      361 tgctcaatga tcttggcgtc cccgcccact gggaggttgg gctctctatg gaacaattgg
      421 ccgagtacgt ggagcaaggg cattacatga ttgccgaggt gaacgcaggc gttctcttcg
      481 atgaggtaca gggtatcggc gacgatcccg agcagtctaa ccatgcgatc gtcatcaccg
      541 gtgttgcgcg ggacctccag accggaagta ttgttggttt ctacgtgaac gataccggtg
      601 ggttctgccg taacagcgat gggagcgccc gcgggggggc cctggtcgac gctgcgcata
      661 tgaagtcggg ctgggctgat accggtggcc tctgcgttat aacagaaccc aggaggagtg
      721 gataagtatg gagagatcgc cgaaagtatt gatcacctat tcagagtgtc gccgccgcca
      781 gaaggtacac catctgaggc aacatgagat ccctatcgag tcccgggtct ctctgccgat
      841 accgactctc aggtggaata ggccgtcgta tgcattcttt gcaggtccgg ggcacaaccc
      901 tcgcgaacca tctcgagggg ctgcaccgcc agaccgctgg tgggctatcg atgcaggcac
      961 aggcaggctg atgctgtacg cacttaccag caccatcccg ttcccctcag gcgctcaacc
     1021 ggaatcggtc gaagcgcctc ctcaggcgag aagcgtcagc gatgtcatcg agaccgtcac
     1081 ccggatcgag aggctgatgg attgtctggt cagagacttc ttctccgggg aacgtggaga
     1141 tccggagcaa aagaacgcgt tgattggtga actgaagcag catctgccgg gcccccttct
     1201 cacgccttac caggtgctgg ccccggattt ctttgcctgg cttgaggaga gccaacccct
     1261 tccggaggaa taaggcatga acagccaccg ggaacaggcc agaacgggtg ccggaataat
     1321 tcccggcacc tcgttcagcc tgaaaggaag atggctcgag aggacgaaga cgttacctga
     1381 cgaacaagat ggaaaacccc gggatattgc gttatgcgcc atccgaaaat cttttgagga
     1441 gtttcgtgag gaggcgaaga aagaggtgcg taccggtgac gccggcaccc tgatgtttcg
     1501 cctgcttgac cagatcaggc agtttatcag gttctatgaa cttgccgaag agccggcgaa
     1561 gagtgctccg gcagatacgg gaccggttga tcggaaagac tccagagaat ccggatatca
     1621 ggaccaggag caaacaggac catctccgca taccgggaga cgtggcatca atcccatgaa
     1681 attgctcggc gctcgcacga aaccggtgga agaagctccc gcgagcgccc cctctttccg
     1741 gagagagcag ttagagatgt tagtcggaaa tatcggcgat tttctggggg tgatggatga
     1801 catgctgccg aaactcctgc caaaacccga acagaagccg gatctccccc gggatagcct
     1861 ggaggcattc cagggcatgc tcgcgatcat gcttgaggcg gatgatcgga gatttacgga
     1921 agcgatgcgc tcgcaaagag aagttatctg cagccttttc ggcaaatatg gcatatcgac
     1981 cgtccttttc gaggaggaac ccgatcgaaa ccgaaaaggt gagtggttcg aggagaggct
     2041 ggtcagcccg gaaaagtttc caaccataag aacagcatcg cctgcactgg tccgcaatga
     2101 tatgttaatc tgccggggta cggtgtatat cccccagagg ccccggtgaa gtagctcttg
     2161 ctacgagttc gatcacaggt gaggtgtatg tccctggata atgacgtgca gctggcacaa
     2221 acttttgatg agatcgataa atccgttctg tcgagtttca cccatatcat cgggttcgat
     2281 ctgggacacg gcgagttctc attgttccag gtatcggacg aacagaacga ttcagccaaa
     2341 tgcatcgaga tcaacaacaa aaaaagccag attacggcta ttgcggtgaa ggataccggt
     2401 gagatcctgg ttggaaataa ggccataacc acctatgatg aggccgtgat cactgtcggc
     2461 ttcaagcgaa aaccgggaga gaaccccaac ctcgatcacc tggtcggact gttctttgca
     2521 ggggtgctga gatctgctct cgcggaacgg aaggtcagcc tgagtaataa aaccctgatt
     2581 ctcgtcggtt gcccctcaga atggacccgg gaacagcaga ggggctacca ggcgattttg
     2641 caggaagcca tatcagattt accatgcgag acgaagccga aggtcgcagt cgttcccgag
     2701 tcccgtgcag ccattcagtg tgcacgacag agcgg
//
LOCUS       DAOQVJ010000198         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44386_length_2723_cov_4.388306, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000198 DAOQVJ010000000
VERSION     DAOQVJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44386_length_2723_cov_4.388306"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKL26_05010"
     CDS             complement(<1..198)
                     /locus_tag="PKL26_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83088.1"
                     /translation="MGVRLGVLALQGDVSEHIRGFRAALAEIGEEEGLVIPVRRPADL
                     DGLSDFDEIARAGEHAPHLRVS"
     gene            complement(188..1084)
                     /gene="pdxS"
                     /locus_tag="PKL26_05015"
     CDS             complement(188..1084)
                     /gene="pdxS"
                     /locus_tag="PKL26_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNS83089.1"
                     /translation="MKLEELRFGTELLKRGFASMQKGGVIMDVVNAEQAEIAEEAGAV
                     AVMALERVPADIRKAGGVARMADPAIIMEIMDAVSIPVMGKVRIGHFVEAQVLESLGV
                     DMIDESEVLTPADEQFHIDKTSFTVPFVCGARDLGEAMRRIHEGAAMIRTKGEAGTGN
                     VVEAVRHMRNIMGEIRALQGKNSQEIVARARELEAPAELLLETVKMGRLPVVNFSAGG
                     IATPSDAALMMQMGADGVFVGSGIFHSSDPRRMARAIVEAVNHFDEPGVIAEVSKGLG
                     QAMKGIDVHTLREDEVLQYRGR"
     gene            complement(1089..2009)
                     /gene="cbiB"
                     /locus_tag="PKL26_05020"
     CDS             complement(1089..2009)
                     /gene="cbiB"
                     /locus_tag="PKL26_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNS83090.1"
                     /translation="MFWIRPLVLPLALLFDRLAGDPHSRLHPVALLGTFIAWWGRPDL
                     YPPCLQRAAGAALWAFTVLLFSLPFILAEIFLPWLVLIVAGPVLLKSCLAWRSLEEHA
                     LSVENALSQGLDEGRSQVQFLVSRDPSVLSREQVHSAAYESVSENLVDSIVSPLCYYA
                     LLGLPGAAAFRAANTMDAMLGYRDHRERLGWFSARADDVLNYIPARLTAGILLCYFAC
                     RGRFAPALDCVRKDGRKRPGFNGGLPMAVMAGGTGTRFEKPGVYSIGPGDRTIEDAGP
                     EILSAVRACTILVTLLLMVLMFLALLIPLI"
     gene            complement(2243..2425)
                     /locus_tag="PKL26_05025"
     CDS             complement(2243..2425)
                     /locus_tag="PKL26_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HNS83091.1"
                     /translation="MKRSLLPILCCPVCKGDLELRVKEENDVEILEGGLYCAACRVEY
                     PIEEGIPDLLPRTHEE"
     gene            complement(2422..>2723)
                     /locus_tag="PKL26_05030"
     CDS             complement(2422..>2723)
                     /locus_tag="PKL26_05030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNS83092.1"
                     /translation="VSDRMGIQEFGTVTHRQRRIGQWDGAMARYSAMVNGCTQAAITG
                     IDRVDKACFGLTNYDKLTKKALEFLEHAEKDIGCPITLISTGPDVSNIIDIRGER"
BASE COUNT          527 a          873 c          795 g          528 t
ORIGIN      
        1 cgatacccgt aggtgcggcg cgtgctcgcc ggcgcgggcg atctcatcga aatccgagag
       61 gccgtcgaga tcggccgggc gcctgaccgg gataaccagc ccttcttctt ccccgatctc
      121 tgcgagcgcc gcccgaaaac cgcggatatg ctcgctcacg tcgccctgga gagccagcac
      181 tccgagtcta acgcccacgg tactggagca cctcgtcctc gcgcagggta tggacgtcga
      241 tccccttcat tgcctgccca aggcccttgc tcacttcggc aatcacgcca ggctcgtcga
      301 agtggttgac cgcctccacg attgcccttg ccatcctcct gggatcgctt gagtggaaga
      361 tgccggatcc cacgaatacg ccgtcggctc ccatctgcat catcagggcc gcatccgatg
      421 gcgtcgctat tcctccggcg gagaagttta cgaccgggag cctgcccatc ttcacagtct
      481 caagaagcag ctctgcggga gcctcgagct cacgggcgcg ggctacgatc tcctgggagt
      541 tcttcccctg gagagcgcgg atctccccca tgatgttcct catgtggcgg accgcctcca
      601 ccacgttccc ggttcctgct tcacccttgg tccggatcat cgccgcacct tcgtgaatcc
      661 ggcgcattgc ctcccccagg tcacgggccc cgcagacgaa cgggaccgta aagctggtct
      721 tgtcgatatg gaactgctcg tcggcggggg tcagcacctc gctctcgtcg atcatatcca
      781 cgccaaggga ctcgaggacc tgggcttcta cgaagtggcc gatacgtacc ttgcccatca
      841 ccgggatgga gaccgcatcc atgatctcca tgatgattgc cgggtctgcc atccgggcca
      901 ctcccccggc cttccggatg tccgcgggaa cccgctccag ggccatgact gcgacggccc
      961 cggcctcctc ggcgatttcc gcctgctccg cgttgaccac atccatgatc acgccccctt
     1021 tctgcatgga cgcaaatccc cgcttcagga gctcggtgcc aaatctcagt tcctcgagtt
     1081 tcatatgctt atataagtgg gatgagcagg gccaaaaaca ttagtaccat aagtagcagg
     1141 gtgacgagta tcgtgcaggc ccgcaccgcc gagagtatct ccggccctgc atcttcaatc
     1201 gtccggtccc cggggccgat ggaatatacg ccaggctttt cgaacctggt cccggtccct
     1261 cctgccatca ccgccatggg gaggccgcca ttaaaccctg gccgttttct cccatccttt
     1321 ctcacacaat ccagcgccgg tgcgaacctc cccctgcatg cgaagtaaca aaggaggatg
     1381 cccgcggtca gccgagccgg tatgtagttc aggacatcgt cggcccgtgc cgaaaaccag
     1441 ccgagccgct ccctatggtc gcggtacccg agcatggcat ccatggtgtt ggctgcccga
     1501 aatgccgcgg cgccgggaag cccgaggagc gcgtagtagc agagcggtga gacgatgctg
     1561 tcaacgaggt tctcggatac cgactcgtac gcggctgagt ggacctgttc ccgggagagt
     1621 actgaaggat ccctgctgac aaggaactgc acctgtgacc taccctcatc gagaccctgc
     1681 gaaagcgcat tctcgaccga tagggcatgc tcctcgaggg accgccatgc gaggcacgac
     1741 ttcaggagta cggggccggc cacaatcagg acaagccacg ggagaaagat ctctgcgagt
     1801 atgaagggca gcgagaagag aagaaccgtg aatgcccaca gtgccgcccc tgcggcacgc
     1861 tggagacagg ggggatagag atcgggcctt ccccaccacg cgatgaacgt cccgaggagc
     1921 gctacagggt gcagcctgct gtgcgggtcg cctgccaggc gatcgaacag gagtgcaagc
     1981 ggcagtacca gggggcggat ccagaacatg cgacgagtcc atccaggaga gaattctcac
     2041 acgatctcac gcggaaagca gaataattat tcgaatattg tgcgtccgaa cattgcgacc
     2101 tgcgatgctg cagtacgaag tacgagggaa aggcccgggg gctcggaggg aggcactgat
     2161 ctattgaaga gcgcgttttt tataaaaaag ggatttgaat cggggtattt tagacctgca
     2221 ccgggggggc cgggttcctt ccttactctt cgtgcgtcct gggaaggagg tccgggatcc
     2281 cttcctcaat gggatactcc accctgcagg ccgcacagta gagccctccc tccaggatct
     2341 cgacgtcgtt ctcttccttc acgcggagct ccaggtcgcc cttgcagacc gggcagcaga
     2401 ggatagggag gaggctcctc ttcatcgttc tcccctgata tcgatgatat tcgagacatc
     2461 cggcccggtg gagatgaggg taatggggca gccgatgtcc ttctccgcgt gctccaggaa
     2521 ctcgagtgct ttctttgtga gcttgtcgta gttggtgaga ccgaaacatg ccttgtccac
     2581 gcgatctatg ccggtaatgg ccgcctgcgt acacccgttg accatcgccg agtagcgggc
     2641 catcgccccg tcccactgcc cgattcgtcg ctgccggtga gtcacggtac caaactcctg
     2701 gatgcccatg cggtccgaga cgt
//
LOCUS       DAOQVJ010000199         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44610_length_2713_cov_4.857035, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000199 DAOQVJ010000000
VERSION     DAOQVJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44610_length_2713_cov_4.857035"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKL26_05035"
     CDS             complement(<1..316)
                     /locus_tag="PKL26_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNS83093.1"
                     /translation="MIADVLILACLAVGIFFNALGIAGLLRFPDVYTRMHATTKMTTF
                     GSMFTALSVIIYGAALFLSSGDGQYVTLSVHTLIAAISLAFTCDGMGPYRVRECKGDR
                     RDQ"
     gene            complement(313..570)
                     /locus_tag="PKL26_05040"
     CDS             complement(313..570)
                     /locus_tag="PKL26_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HNS83094.1"
                     /translation="MIDPWYIAVCILVILVCLSLIRLFLGPTAADRAVAIDTVNTLSV
                     AVLILLGVAYQQIIFIDVAIVYALLSFVSTLYIAKYLGGEL"
     gene            complement(567..1094)
                     /locus_tag="PKL26_05045"
     CDS             complement(567..1094)
                     /locus_tag="PKL26_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNS83095.1"
                     /translation="MIPFLLTFILALLLYLLLAAGSGSVLLWSPSELVAGVALSLLAA
                     AVARNLLCRSDSYRMANPLRLLLIPLYLLGPFLLEVTKANLDVAYRVITGNIRPGIIR
                     VQSGLRTDLGIFMLANSITLTPGTITVDIDEKSHDLYVHNINIPQGWEERPQVEEREL
                     FSLANLPAWIRRIAE"
     gene            1453..>2713
                     /locus_tag="PKL26_05050"
     CDS             1453..>2713
                     /locus_tag="PKL26_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNS83096.1"
                     /translation="MLWWCDSCHTPVLANVCSRCSTRAREVMITPPADARPAFPSDIS
                     HVNRVYSEHFGVPLIPPGHIVLLNKVPDPDRMEEIIVGGGVVGSIKYLPGESRWEAIP
                     RPQASVFMTPRRKFVVVDNGAVPSIQQEGASVLAPGLVEIEPSVSRGDEVFVLNEERA
                     VVAVGRAKVDAKEAGNMERGAIVRTRRNFPSNCTRGEATWEDAVEASAGVLEEQEAEA
                     ISFIRDVAARNPLPVNVSYSGGKDSLATLLLVNKALGKVPMLFADTGLEFPETCRNVE
                     EVSREFGNPVVRARGEEAFWKAFQVHGPPAVDFRWCCRTAKLNPVEKVIREHWGECLS
                     FIGQRKYESFRRMASGRVWRNPHLKSQLSAAPIHHWTALQVWLYLFRENAPYNPLYES
                     GLDRIGCFMCPSSDMAVLRQIEESHPLL"
BASE COUNT          628 a          738 c          808 g          539 t
ORIGIN      
        1 cctgatctct gcgatctccc ttgcattcac gaacgcggta gggtcccatg ccatcgcacg
       61 tgaatgcaag ggagatcgca gcgatcaggg tatgaacaga gagcgtcaca tactgcccgt
      121 ccccgctcga aaggaacagc gccgcaccgt agatgatcac cgagagggcg gtgaacattg
      181 acccgaatgt ggtcatcttg gtcgtggcgt gcatcctcgt atagacgtcg gggaaccgga
      241 gcagccctgc gattccgagc gcattgaaga atatcccgac cgcgaggcat gccaggataa
      301 ggacatccgc gatcacagct cgcctcccag gtacttggca atatagagcg tgctcacgaa
      361 ggagagcaat gcatagacaa tcgccacatc gatgaagatg atctgctggt aggccacgcc
      421 gaggagtatc agaacagcga cagagagcgt gttgacggtg tctattgcca ctgcacggtc
      481 agcagcggtc ggtcccagga acaaccggat cagggaaagg cagaccagga tgacgaggat
      541 gcacactgcg atataccagg ggtcgatcat tccgcaatcc tccgtatcca ggcggggagg
      601 ttcgcaagcg agaaaagctc tctctcctca acctgcggcc tttcctccca cccctgtggg
      661 atattgatat tatggacgta gaggtcgtgg ctcttctcgt cgatgtcgac agtgatggtg
      721 cccggggtga gtgtgatgga atttgcaagc atgaagatgc cgaggtcggt ccttaagccc
      781 gactgcaccc tgattatccc gggccggata ttcccggtga tcacgcgata ggccacatcg
      841 aggtttgcct ttgtcacttc gaggaggaat ggcccgagaa ggtatagtgg gatgagaaga
      901 agccgcagcg ggtttgccat ccggtaactg tccgacctgc agagtaaatt ccttgcgact
      961 gcggcggcaa gaagcgagag cgcaacacct gctaccagtt cggaaggaga ccagagcagg
     1021 acgctcccgc ttccggcggc aagcaggaga tacagcagta gggcgagaat gaacgtgagc
     1081 aggaaaggta tcatccggac ctcccgcacc tgtcgccccg gtattcatta cgtgcaccaa
     1141 tcatcatgga gatcacagga cgcctgaatc aggatataac tatcgggccg aatagacccg
     1201 aagaccacac atcattatgt ataatctatc atgatcgatt atgtaaatca agggacataa
     1261 acaatactgt ttgtggcaat gcctaaaacg atttttacgt gatttattgg aattaaaacg
     1321 agatttacac gccctgtgcg gtggtccatc gaacttttgg tcaggcagaa gaggaaggca
     1381 gctacctgtg gatatttatg gaaatatctg caaaaagtac ccccaatgcg ctcgccatca
     1441 cttcgccgga ggatgctctg gtggtgcgat tcctgccaca cccctgtcct ggccaatgtc
     1501 tgctcccgct gcagcacccg cgcccgcgag gtcatgatca ccccgcctgc cgatgcgagg
     1561 cctgcgtttc cttccgatat ttcccacgtg aacagggtat actcggagca tttcggtgtt
     1621 ccactcattc cccccgggca cattgtgctg ctgaacaagg tgcctgaccc ggatcgaatg
     1681 gaagagatca tcgtgggagg gggcgtggtc gggtcgatca agtatctccc tggggagtca
     1741 aggtgggaag ccatcccccg tccacaggca tccgtcttca tgacaccgcg tcggaagttc
     1801 gtggtcgtcg acaacggcgc ggtcccgtcc atacagcagg agggggcaag cgtccttgca
     1861 ccaggactcg tcgagatcga accatctgtc tcccgcggag acgaagtctt tgtccttaac
     1921 gaggagagag ccgttgtagc cgtcgggcgt gcaaaggtgg atgcaaagga ggccgggaac
     1981 atggagaggg gtgcaattgt ccggacaagg cggaacttcc cttcgaactg cacaaggggg
     2041 gaggcgacgt gggaggatgc agtcgaggca tctgccgggg tactggaaga acaggaggca
     2101 gaagcgatct cgtttatccg ggatgtcgcc gcgagaaatc ctctcccggt gaatgtctcg
     2161 tactcgggtg gaaaggacag cctcgcaacc ctccttctcg tgaataaagc gcttggcaaa
     2221 gtacccatgc tctttgcaga cacggggctt gaattcccgg aaacctgccg caatgttgag
     2281 gaagtctccc gcgaatttgg gaacccggtc gtgcgggcac gcggcgaaga agccttctgg
     2341 aaggcgttcc aagtgcacgg gcctcccgcg gtcgacttca ggtggtgctg ccgcaccgca
     2401 aagctgaacc cggttgagaa ggtgatcagg gagcactggg gtgaatgcct ctcattcatc
     2461 ggtcagagga aatacgagtc gttccggcgg atggcaagcg ggagggtgtg gagaaacccg
     2521 cacctcaaaa gccagctctc tgcagctccc atccatcact ggacggcact gcaggtctgg
     2581 ctgtacctgt tcagggagaa cgcgccctat aacccgcttt acgagtccgg gcttgaccgg
     2641 attgggtgct tcatgtgccc ttccagcgat atggccgtac tccggcagat cgaagagagc
     2701 caccctctgc tct
//
LOCUS       DAOQVJ010000200         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44694_length_2710_cov_4.177778, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000200 DAOQVJ010000000
VERSION     DAOQVJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44694_length_2710_cov_4.177778"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKL26_05055"
     CDS             complement(<1..445)
                     /locus_tag="PKL26_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HNS83097.1"
                     /translation="MQGAREQEIQKLARLAAERGAEARVISSRDVIVSDWVRFKCRFG
                     CKGYGKHLSCPPYAPAPEETRRMVREYETGLLLRFEGIPGHRDLSPDQIPEDFHPFFR
                     DLILWVNGTVHFLEKTAFYDDFYKAFGFGAYPCIYCEHEHCVAEEQ"
     gene            541..1410
                     /locus_tag="PKL26_05060"
     CDS             541..1410
                     /locus_tag="PKL26_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS83098.1"
                     /translation="MEQTGEIIRDQQVQADGTAYRVVVREEDLSRFYPGMLRYTLEAW
                     AGPEVLAQFRTNTYEYSPAMPFHARQVAEERAASWEAELRADPGVFCESHPAPSLPGG
                     RVQDGRIVIIQGSPRPGGNSAILASWAAEAARREGREIEVIYPHDMDIHPCIGCYQCY
                     NTGTCVFQDDMNEIIDAVAKCRLLVICSPVYTNTVPAGLKALLDRFQAFHAEMTLGGH
                     LLLRKGLLMAVAGRKGQDNFMCVTEVIRAFFSLLGITPLQPVLVDATDVIRDVTKVEG
                     LEDRVRYLVRENL"
     gene            complement(1433..2116)
                     /locus_tag="PKL26_05065"
     CDS             complement(1433..2116)
                     /locus_tag="PKL26_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNS83099.1"
                     /translation="MCGRFTITLTVGFGERFGVDESSLDLVPRYNIAPSQQVPVIFTT
                     PAGARAAMPMTWGLVPSWTRDMTGARPLINARADSLAERPAFRGPLARHRCIIPATGF
                     YEWQKSGTQKVPYYICRKDKGFLAFAGLYDVLRGRNPPLWTFVIITTEPNPLVARFHD
                     RMPAILTRENEDRWLTPGPIAERDRASMLAPCADPILEAYPVSRAVNDPSRDGPDLIR
                     MSGDRTLDV"
     gene            complement(2089..>2710)
                     /locus_tag="PKL26_05070"
     CDS             complement(2089..>2710)
                     /locus_tag="PKL26_05070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNS83100.1"
                     /translation="GVGDADLLFSYFLRRTRGFEDPGNILDFLVDEGVYSDALFDVCS
                     AMYRDVKLSHIEPYAGVEKTLERLVEREYPLALVTDAHRNDAIPRLEKAGLRDYFDQV
                     VTYDMTWKKKPDPVPFLYALRRMNTAPGDAMIVGDSPRRDIAPGKDLGMTTVYARYGD
                     RFSSSRNKGRADFTIDEFAQLIPLLSGLEQRGKGRRSPCAAGSPSR"
BASE COUNT          559 a          791 c          793 g          567 t
ORIGIN      
        1 gttgttcctc tgcgacgcag tgctcgtgct cgcagtagat gcacgggtat gcgccgaacc
       61 cgaacgcctt gtaaaagtcg tcatagaatg cagtcttctc aaggaaatgg acggtcccgt
      121 ttacccagag gatcagatcg cggaagaacg ggtgaaaatc ctccgggatc tggtccgggg
      181 agagatccct gtgcccggga atcccttcga atctgaggag aagcccggtt tcgtattccc
      241 tgaccatccg ccgggtctcc tcaggtgccg gtgcatacgg cggacagctc aggtgctttc
      301 cgtacccctt gcacccgaaa cggcacttga accggaccca gtcagagacg atgacgtccc
      361 gcgaggagat gacgcgggct tcggcccctc tctccgccgc caggcgggcc agtttttgaa
      421 tctcctgttc acgcgccccc tgcattgcac attcccagtg gagaattctt caggttccgt
      481 cccataaaag tatcacttcc gactggaacg tatatccctc accactccta caactctttc
      541 atggagcaga ccggagagat aatccgggac cagcaggtgc aggcggacgg aactgcatat
      601 cgtgtcgtcg tccgtgagga ggatctctcc cggttctatc ctgggatgct gaggtatacc
      661 ctcgaggcct gggccgggcc ggaggttctc gcgcagttcc ggacaaacac ctacgaatac
      721 tcacccgcca tgcctttcca tgccaggcag gttgcagagg agagggccgc ctcatgggaa
      781 gcggagttgc gtgccgatcc tggtgtattc tgcgaatcac atcccgcacc ttcactgcct
      841 ggcgggcgcg tacaggacgg gcgtatcgtg atcatccagg gaagcccccg cccgggagga
      901 aacagcgcca tccttgcatc atgggctgcg gaggctgcgc gccgcgaggg gagggaaata
      961 gaagtcattt acccccatga catggacatc cacccctgca tcgggtgcta ccagtgctac
     1021 aacaccggta cctgcgtctt ccaggatgat atgaatgaga tcattgatgc ggtcgcaaag
     1081 tgcagactgc tcgtcatctg ctcgcctgtg tataccaaca ccgttcctgc gggcctgaaa
     1141 gcccttcttg accgttttca ggcatttcac gcagaaatga cccttggagg ccatcttctt
     1201 ttgcggaagg gattgctgat ggccgttgcc ggacgaaagg gccaggacaa tttcatgtgc
     1261 gtgacagaag tgatcagggc gtttttctcc cttcttggca taactcccct gcagcccgtt
     1321 ctcgttgatg caacagacgt gatacgcgat gtgacgaagg tggaaggtct cgaagatcgt
     1381 gtaaggtact tagtaaggga gaatctgtaa gaaaaatacc aggaaggtcg tgtcacacgt
     1441 cgagcgtgcg atcccccgac atccggatga gatcaggccc gtcccgggag ggatcgttca
     1501 ctgccctcga cacggggtat gcttcaagaa tgggatcggc gcagggtgcg agcatggatg
     1561 cacggtcccg ctccgcgatg gggccgggtg tcagccagcg gtcctcgttc tcgcgcgtaa
     1621 ggatggcagg catccggtcg tggaaccgtg ccacaagcgg gtttggttcc gtggtgatga
     1681 ttacaaacgt ccacagagga ggatttcgac ctctgaggac atcatacagc cctgcaaagg
     1741 caaggaaacc cttgtccttc ctgcagatgt agtagggcac tttctgggtt ccggacttct
     1801 gccactcgta gaagccggtt gcagggataa tgcatcggtg ccgtgcaagc ggccccctga
     1861 atgcggggcg ctccgcgagt gagtcggccc gtgcattgat cagtgggcgg gctccggtca
     1921 tatcgcgcgt ccatgagggg acaagtcccc aggtcattgg catagccgcc cgggctcctg
     1981 ccggggtggt gaagataacc ggtacctgtt gggagggtgc gatgttgtaa cgcgggacaa
     2041 gatcgaggga agattcgtcg accccgaacc gctcgccgaa ccctactgtc aacgtgatgg
     2101 tgaacctgcc gcacacggcg atctcctccc tttccctctc tgttccaggc ccgatagcag
     2161 cggaatcagc tgggcgaact cgtcaatggt gaaatctgca cgccccttat tcctcgagga
     2221 tgagaaacgg tccccatagc gggcatacac ggtggtcatt ccaagatcct ttcccggcgc
     2281 gatatccctt ctcgggctgt cccccacgat catggcgtct cccggcgcgg tattcatcct
     2341 ccggagggca taaaggaatg gcacagggtc cggcttcttc ttccatgtca tgtcgtaagt
     2401 gaccacctgg tcaaaataat cgcgcaggcc ggccttctca agccggggga tcgcgtcatt
     2461 cctgtgggca tcggtgacga gggccagggg atattccctt tcgacaaggc gctccagcgt
     2521 cttttcgact cccgcataag gctcgatgtg cgaaagcttc acgtcgcggt acatggcgct
     2581 gcacacatcg aacagggcat ccgagtatac cccttcatca acgaggaagt ccagtatatt
     2641 cccagggtcc tcgaagcccc ttgtccgccg gagaaaatac gagaagagca gatcggcatc
     2701 tccaactcct
//
LOCUS       DAOQVJ010000201         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44865_length_2703_cov_4.522281, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000201 DAOQVJ010000000
VERSION     DAOQVJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44865_length_2703_cov_4.522281"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKL26_05075"
     CDS             complement(<1..336)
                     /locus_tag="PKL26_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS83101.1"
                     /translation="MSIYTVCQKSVVSVTPDTSIRFVADLMRGKNLGCVVVAAEQKPV
                     GIVTDRDLALRRDRICGEEADEAPVSTIMTREVCTIRKDTGIFDAIQEMKSSGVRRLP
                     VADAGGRLVG"
     gene            complement(387..1157)
                     /locus_tag="PKL26_05080"
     CDS             complement(387..1157)
                     /locus_tag="PKL26_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS83102.1"
                     /translation="MIPRPTWNAWKVWRRNWDVFFKTYRVNFLPPFIEPVLYLLAIGY
                     GLGMFVGEIEGIPYVLYIAPALLAISMMNSSFFECTYSSFVRMYYQKMFDAMVSTPLS
                     IEDVIAGELLWGATRSFIYATAMLVVLVAFGVVSLPTSLLVIPFSFLAGLLFAGIAMC
                     FTAITPGIDTLNYPAFLFITPMFLFSGTFFPLSVLPGPLQMFALGVLPLAQVVTVTRS
                     LTLASLSPLFLYSLAWIGAVTAVFFFLSIYLMKRRLVV"
     gene            complement(1164..2072)
                     /locus_tag="PKL26_05085"
     CDS             complement(1164..2072)
                     /locus_tag="PKL26_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS83103.1"
                     /translation="MQPIIEARGLRKDFGALKAVDGISFSVMPGEVFGFLGPNGAGKT
                     TTMRMIECVSPATAGMLTVFGIDVNRDAREIKRRLGVVPQETNLDPDFTCFENLVVYA
                     RYFGIPRAEARERAEELLSFVQLSEKRDVPIEKLSGGMKRRLILARALINSPGLLVLD
                     EPTIGLDPQARHLMWEKLKALQAAGNTIVLTTHYLEEAERLCNRLVIMDHGKILIEGT
                     PAELVHRYAGEEVVEVDHTPEVVACLQEMGARYEVFGDMIEVFTDQPREVAKRLLSDC
                     NPGRVFVRRGSLEDVFLSLTGRVLRD"
     gene            2213..>2703
                     /locus_tag="PKL26_05090"
     CDS             2213..>2703
                     /locus_tag="PKL26_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83104.1"
                     /translation="MDSRHIVRQMLGIESVRRQAILSVGANVGITAIGYLATVYIAHT
                     ADAEVLSAYFLFLAHYSLAALGADGGMGGAAVKLISEGREKDSYFTAQAVARLMLLAM
                     CPGLIVVVVAPFMRGFTGAGLLPWLLMALATLASRGQRGYRSARNPRSSPLDKYHMVE
                     ESNP"
BASE COUNT          567 a          785 c          812 g          539 t
ORIGIN      
        1 gccgacgagg cggccccccg catccgcgac aggcagccgg cggacacccg acgacttcat
       61 ctcctggatg gcgtcgaaaa tgcctgtgtc cttcctgatg gtgcagacct cgcgggtcat
      121 gatggtggag acgggtgcct cgtccgcctc ctctccgcag atgcggtctc ttcttaaggc
      181 caggtccctg tcagtgacga tgccgacggg cttctgctct gctgcaacga ccacgcaccc
      241 gaggttcttg cccctcatca gatcggccac gaaacggatc gaagtgtcgg gcgtgacgct
      301 taccacgctc ttctggcaga cggtatagat gctcatttga gttcctgtct cctactgtgt
      361 gcatcgatag gataaaaatg accctttcag accacgagcc ttctcttcat caggtagatt
      421 gagaggaaga agaacacggc ggtaaccgcg cctatccacg caaggctgta cagaaagagg
      481 ggggagagcg atgcgagagt gagcgagcgg gtgacagtga ccacctgggc caggggaagg
      541 acaccgaggg cgaacatctg gagcggcccg gggaggaccg agagggggaa gaaagtcccc
      601 gagaagagga acatgggggt aatgaagaga aacgccgggt aattcagggt atcgatgccg
      661 ggagtgattg cggtaaaaca catggcgatg cctgcaaaga ggaggccggc gaggaacgag
      721 aaggggatga ccaggagtga agtgggcagt gataccaccc cgaatgcgac gaggaccacc
      781 agcatcgctg ttgcgtaaat gaagctccgt gtggcacccc agaggagctc gcctgcaatg
      841 acatcttcga tagagagggg agtcgagacc attgcatcga acatcttctg gtagtacatc
      901 cgcacaaacg atgagtaggt acattcaaag aacgaggagt tcatcatcga gatggcgagg
      961 agtgcgggcg cgatatagag gacgtatggg atcccctcga tctctccgac gaacattccg
     1021 aggccgtacc cgatcgcaag gagatagagc accggctcga tgaagggggg gaggaagttc
     1081 acccggtagg tcttgaagaa gacatcccag ttcctccgcc atactttcca ggcgttccat
     1141 gtaggtcggg ggatcatcaa gcatcagtcc cgaagaaccc tccccgtaag ggaaaggaag
     1201 acgtcttcga gcgatccgcg gcgcacgaac actcttcccg ggttgcagtc cgaaaggagc
     1261 ctctttgcga cctccctcgg ttggtccgta aagacctcga tcatgtcccc gaacacctcg
     1321 taccgggcgc ccatctcctg caggcaggcc acgacctcgg gggtgtggtc cacctccacg
     1381 acctcctcac ctgcgtatcg gtgcacgagt tcggcggggg ttccctcaat gaggatcttt
     1441 ccatggtcca tgatgaccag gcggttgcag agccgctcgg cctcctcaag gtagtgagtg
     1501 gtgagcacga tggtgtttcc tgcagcctgg agagccttta acttctccca catcaggtgt
     1561 cgggcctggg ggtcgaggcc gatggtcggc tcgtcgagga ccagaagccc cgggctgttg
     1621 atgagcgccc ttgcaaggat gagcctccgc ttcatccctc cagagagctt ctcgatgggc
     1681 acgtccctct tctccgagag ctggacgaag gagaggagtt cttctgccct ctctcttgcc
     1741 tcggcccggg gaatcccgaa gtagcgggcg tatacgacaa ggttctcgaa acaggtgaaa
     1801 tcagggtcga ggttcgtttc ctgggggacg actccaagcc tccgcttgat ctcccttgcg
     1861 tccctgttca catcgatgcc aaagaccgtg agcattccgg ccgttgccgg agatacgcat
     1921 tcgatcatcc gcatggtggt ggtcttgccg gcgccgtttg gcccgagaaa tccgaagacc
     1981 tcgccgggca tcaccgagaa gctgatcccg tccacggctt tcaatgcccc aaagtctttc
     2041 ctgagccccc gcgcctcgat tatcggctgc acagacagat cacacctgca tggaaaggat
     2101 ttgtccaccc ggccacaaca ctctttcggt gaagacaggg gattctcatc cttctctccc
     2161 ggccaggaga ggtcatctaa aagtggtcca tcagcgttca ataagaggat tgatggactc
     2221 ccgacacatc gtacgccaga tgcttgggat cgaatccgtc cgccgtcagg ctatcctctc
     2281 cgttggggcc aatgtgggga ttactgccat tgggtacctg gcaacggttt atatcgccca
     2341 taccgcggat gccgaggtgc tgagtgccta cttcctcttc ctcgcccatt acagcctggc
     2401 ggccctcggg gctgacggcg gcatgggggg cgcagcggtc aagctgatca gtgaagggag
     2461 ggaaaaggat tcatacttca ccgcacaggc cgtcgccaga ctcatgctcc ttgccatgtg
     2521 ccccggtctc atagtcgtcg tggttgcacc cttcatgagg ggcttcactg gtgctgggct
     2581 ccttccctgg cttctcatgg cgcttgccac cttggcctcc cgtggccagc gcggatatag
     2641 gagtgcccgc aatccccggt catctccctt ggataaatac catatggtgg aggagtctaa
     2701 ccc
//
LOCUS       DAOQVJ010000202         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44883_length_2702_cov_3.701171, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000202 DAOQVJ010000000
VERSION     DAOQVJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44883_length_2702_cov_3.701171"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..167
                     /locus_tag="PKL26_05095"
     CDS             60..167
                     /locus_tag="PKL26_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83105.1"
                     /translation="MLNEECERLGLTARVTRIRVWESPTCHAEVIYEGQ"
     gene            154..762
                     /locus_tag="PKL26_05100"
     CDS             154..762
                     /locus_tag="PKL26_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS83106.1"
                     /translation="MKVSEIFRSIQGEGIRQGRPCTFIRFAGCNLNCSWCDTPQARIG
                     GREMDAGEINARVRELGGTYLCITGGEPLLQGAPLLSLLSSFHSSGYMIDIETNGTLD
                     FSPFQALASICMDVKCPSSGEESDLSLLSRITTGDSVKFVVRDRTDCRYAVDVLMEHP
                     VRGEVFFSPVEGSDARAIARFLIDEDLPARLQLQLHKILGVC"
     gene            766..1437
                     /gene="queC"
                     /locus_tag="PKL26_05105"
     CDS             766..1437
                     /gene="queC"
                     /locus_tag="PKL26_05105"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNS83107.1"
                     /translation="MRAVCLLSGGMDSTTLAYLAREMGFRILALHFNYGQRTEKKERS
                     AARTIAALLEAEEFREVDLGYLSSFGASSLTDHSVPVDRYNPEGLDVPNTYVPFRNGN
                     LLSIATSYAEARGAEAIFIGVQSQDYSGYPDCRPAFIDAFQRVIDTGTRGETSIRLHT
                     PFIHMNKKEILEEGMRLKVPYEHTWSCYQDEDAACGACGACHFRKAAFSALGITDPIP
                     YREEE"
     gene            1434..1943
                     /locus_tag="PKL26_05110"
     CDS             1434..1943
                     /locus_tag="PKL26_05110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNS83108.1"
                     /translation="MTDLYRGKRLWVDRKEYALPNGSKVEKVAVHPGNAVVILPFRAD
                     GSCYLIRQYRFVIGEYILEAPAGTLNPGEDPVTAAHRELIEETGFQAETLIPRGSIYT
                     TPGFSDEVLHLYEAHGLSPSCLYEKDDDEIIELIRVRSEDLPGMISDGRICDAKTISI
                     ICRCIGATR"
     gene            2441..2572
                     /locus_tag="PKL26_05115"
     CDS             2441..2572
                     /locus_tag="PKL26_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83109.1"
                     /translation="MYLCYAMEESSGGRYAAIAVALDPEFAGFIGVSTSGFSRAGDS"
BASE COUNT          538 a          810 c          826 g          528 t
ORIGIN      
        1 cggtcatcac gacaccagga gagcccacga gcgaactttt ggcaaccctg atcgcgggga
       61 tgctcaacga agagtgcgaa aggctgggtc tcacggcaag ggtgacccgt atccgcgtct
      121 gggaatcccc cacctgccat gcagaagtga tatatgaagg tcagtgagat attccggagt
      181 atccagggag aggggatacg ccaggggagg ccctgcacct tcatcaggtt cgccggatgc
      241 aacctcaact gctcatggtg cgacaccccc caggcccgta tagggggaag ggagatggac
      301 gcaggcgaga tcaatgcccg ggtgcgggaa ttgggcggca cctacctctg catcaccggg
      361 ggagagcctc tcctccaggg tgcaccgctc ctctcccttc tctcatcctt ccattcctcc
      421 gggtacatga ttgacatcga gaccaatggg actcttgact tctcgccctt tcaggcgctc
      481 gcgagcatct gcatggacgt gaagtgccct tcttcggggg aggagagcga cctctcgctc
      541 ctctcccgca tcaccacggg tgactcggtc aagttcgtgg tccgggaccg gacggactgc
      601 agatatgcgg tggatgtgct catggagcat cccgtccgtg gggaggtctt tttctccccg
      661 gtggaaggat cagacgcacg cgccatcgcc cgcttcctga ttgatgagga cctgccagcg
      721 cggctgcagt tgcagctcca caagatcctg ggggtgtgct agcgcatgcg ggcagtgtgc
      781 ctgctctcag gggggatgga ctcgacgacc ctggcctacc tggcaaggga gatgggcttt
      841 cgaatcctcg cgctccactt caactatggc cagcgaacag agaagaagga gcgttccgct
      901 gcaaggacga ttgcggcgct tcttgaagcg gaagagttcc gagaggtcga tctcggctat
      961 ctctcatcct ttggagcgag cagcctgacg gatcactcgg tccctgtcga ccgctacaat
     1021 cctgaaggtc tcgatgttcc caacacctat gtcccgttcc ggaacggaaa cctcctctcg
     1081 atcgccacga gttacgcgga ggccaggggt gcagaggcaa tcttcatcgg tgtccagtcc
     1141 caggactaca gcgggtatcc cgattgccgg ccggcgttca tcgacgcgtt ccagagagtg
     1201 atcgacacgg ggacccgtgg cgaaacatcc atccggctcc atactccctt tatccatatg
     1261 aacaagaagg agatcctcga ggaggggatg cgcctgaagg tcccctacga gcatacctgg
     1321 tcatgctacc aggacgagga tgcagcctgc ggtgcctgcg gcgcctgcca tttccggaag
     1381 gccgcgttct ctgcgctcgg gatcacggat cccattccgt accgggagga ggaatgaccg
     1441 atctttacag gggaaagagg ctgtgggtcg acaggaagga gtatgccctt cccaacggct
     1501 caaaggttga gaaggtggcg gtgcacccgg ggaatgccgt ggtcatcctg ccattccggg
     1561 cagacggttc ctgctacctc atcaggcagt accggttcgt gataggggag tatatcctcg
     1621 aagcgcccgc agggaccctg aaccccggcg aggaccccgt caccgccgcg caccgcgagc
     1681 tgatcgagga gaccgggttc caggcagaga cgctcatccc caggggctct atttatacca
     1741 cgccgggttt ctctgacgag gttcttcacc tctacgaggc gcacggccta tccccctcct
     1801 gcctttatga aaaagacgac gacgagatca tcgaactcat cagggtaagg agtgaggatc
     1861 tccccgggat gataagtgac ggcaggatct gcgacgcgaa gacgatcagc atcatctgcc
     1921 gctgtatcgg ggcaacgcgg tgagacgtgc tcttttgtat cccctgcctt gcacttgccc
     1981 tgctccctgt gtttgcgcgg tgcgtggatg agcgcggcag gctatccggg gattatctga
     2041 taaacgagca tggctgcctc tccctggaat gcccggcatg caccttagaa gagaagtcct
     2101 tcccaggaga gggagggtat tcagtcaccc gggtggcatt ccataccccc gagggagatg
     2161 tcgatgcttc gccgcgtttc ctgacgttcc tgtcgcaggc ttcgtgctcg cacccggcgc
     2221 aacaggaaga aggaaagaca ccgcaacagg gcagaggcgt tcgcacggtc cggttacgct
     2281 ttcctggtca gtgacatgag gggaaacggc ggcgagaccg gggggcaccc cctggacctt
     2341 gagcgcgatt accagaggtt ccttgcccac gagtggcccc aatactatct ctcaatatgc
     2401 gacatccagt gcgccagggc atttctgcag gagaagactc atgtacctgt gttacgccat
     2461 ggaggagagc agcgggggac ggtacgcggc cattgctgtg gcactggacc cggagtttgc
     2521 agggttcatc ggtgtctcga cctcgggatt ctcccgtgcc ggagactcgt aactcaggcg
     2581 atctccggcg gttcctcctc tcaatcgacc ccgatgcctg tacagaacgc atggcacccc
     2641 ggaacatatg gatactccac tcgcccgggg atacggtcat ccccttcgcc gacgggcagg
     2701 ca
//
LOCUS       DAOQVJ010000203         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_44993_length_2697_cov_4.127555, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000203 DAOQVJ010000000
VERSION     DAOQVJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44993_length_2697_cov_4.127555"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKL26_05120"
     CDS             <1..289
                     /locus_tag="PKL26_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HNS83110.1"
                     /translation="IREPSLVILDEPTGTMDPLTKRDVKHSILHARQEMDQTFILVSH
                     DMEFVQEVCDRVAWMKGGKVVALGSTEEVLAEMPAEDRGMQGLAGQPSPAL"
     gene            552..1433
                     /locus_tag="PKL26_05125"
     CDS             552..1433
                     /locus_tag="PKL26_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS83111.1"
                     /translation="MTDIPREEYILKCTSACAGCSDSLTLRYVLKAAGPETVLVVPAC
                     CTSVIQGIYPQTAMNIPVYNVAFAAAAACASGMSAAFEAAGKKTNVIVYAGDGGTVDI
                     GIQALSGAFERGANFLYICYDNEAYGNTGMQRSGATPAGARTTTTPAGKGQSKKDLDA
                     IVAAHNPPYMATACASYPKDLFSKVQKALSIRGPKFIHVLAPCPPGWRYSSERSIEMG
                     RLAVRSGMWILYEREYGKVTINGPSKAAMIRPIPLEDYLDAQGRFKGICPEAVAMLRS
                     LAERNAKRLKREEEGIC"
     gene            1427..2539
                     /locus_tag="PKL26_05130"
     CDS             1427..2539
                     /locus_tag="PKL26_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS83112.1"
                     /translation="MLTVATGNKAVAAAVKDACPSVVAAYPITPQTEIVEQIAEYVTS
                     GELKSQYIPVESEHSAMAACIGASITGVRTFTATSSHGLHYMHEMVNWAAGARLPIVM
                     ANVNRALGPGWNIWAEHTDAFQERDTGWLQVYVGSVQEAYDATLMAFRIAEHESVLLP
                     VMVNLDGFLLSHSMHPLQSVEIRDFIPPIHLPHAIDTANPAGYGTLTGPDQYFRFRWD
                     IERSMRDSVNVIRETEEDFRHRFGREYHPVEEYRCDDADVVVVAMGTLGKEAEVAVDL
                     LRKEGVKAGSMRIRWFRPFPRLDLADRDVVVIDRDYSFGFGGVIARSLAATEGANPFS
                     VIAGLGGQEVTYDDIAGFVRDRRPGEEFWFGVTPDV"
     gene            2532..>2697
                     /locus_tag="PKL26_05135"
     CDS             2532..>2697
                     /locus_tag="PKL26_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNS83113.1"
                     /translation="MYEIRIHSRGGQGGVTAARLLALAAIRDGKYATACPFYGAERRG
                     APVVSYVRIDD"
BASE COUNT          560 a          785 c          850 g          502 t
ORIGIN      
        1 gatacgcgag ccgagcctcg tgatactcga cgagccgacc gggacgatgg accctctcac
       61 aaagcgggac gtgaagcatt cgatcctcca tgcccggcag gagatggacc agacctttat
      121 cctcgtctcc catgacatgg aattcgtgca ggaagtgtgt gaccgggtgg cctggatgaa
      181 gggaggaaag gtggtggcac tcgggagcac ggaggaggtc cttgcggaga tgccggccga
      241 ggacaggggt atgcagggcc ttgcgggaca gcccagcccg gcactctgaa cccttcgaga
      301 agggttcttt ggcctgatac gcccactttt ttttttacta tgaaaatggc tgcgccattt
      361 tcataaatgc gtatgcgtgt gtctcgaaga gaatcatgta gtttttcgcc atatctcccc
      421 atccagttgc cgggcaggat gatttcccct gctcataagc gattcttatc gaaaatatgg
      481 gcaatttcct gtttttttcg aattaaatat accttattac agatggcgga caacatttac
      541 tgctgagttt gatgacggac attcccaggg aagaatacat tctgaaatgt acttcggcat
      601 gcgcggggtg cagcgactcg ctcactctcc ggtacgtgct gaaggcggca ggacccgaga
      661 ccgtgctggt ggtccctgca tgctgcacca gcgtgatcca gggcatctat ccccagactg
      721 ccatgaatat ccccgtatac aatgttgcct tcgccgcggc ggccgcctgc gcctcgggga
      781 tgagcgcggc attcgaggcg gcggggaaga agaccaacgt gatcgtctac gccggtgacg
      841 ggggaaccgt ggacatcggc atccaggccc tctcgggtgc gttcgagcgg ggtgcaaatt
      901 ttctctacat ctgttacgac aacgaggcat acggaaatac aggaatgcag cgctccggtg
      961 caacccccgc gggagcgagg accaccacca cccctgcagg gaaggggcag tccaagaagg
     1021 acctggacgc gatcgtcgcc gcgcacaacc ccccttacat ggccactgcg tgcgcctcat
     1081 acccaaagga cctcttctcg aaggtgcaga aggccctctc catccgcggg ccgaagttca
     1141 tccatgtcct cgctccctgc ccaccggggt ggcgctactc ctctgaaaga agcatcgaga
     1201 tgggcaggct cgcggtgaga tctgggatgt ggatcctcta tgagcgcgag tacgggaagg
     1261 tcaccatcaa cggaccctcg aaggcggcaa tgatcaggcc cataccgctc gaggattacc
     1321 tggatgcgca ggggaggttc aagggcatct gccccgaagc agtggccatg ctgagatcgc
     1381 tcgccgagcg gaatgcaaag cgcctgaaga gggaggagga agggatatgc tgacggtcgc
     1441 aaccgggaac aaggccgtag ctgcagcggt gaaggatgcc tgcccgtccg ttgtcgcagc
     1501 gtatcccata accccgcaga cagagatagt ggagcagatc gcggaatatg tcacttcggg
     1561 ggagttaaag agccagtaca tcccggtgga gagcgagcac tcggccatgg cggcgtgcat
     1621 aggcgcgagt atcacaggag tgcggacttt caccgcgacg agctcgcacg gtctccacta
     1681 catgcacgag atggtcaact gggccgcggg agcacggctc cccatcgtca tggcaaacgt
     1741 gaaccgcgcc ctcgggccgg ggtggaacat ctgggccgag cacaccgatg cgttccagga
     1801 gagggacacg ggatggctcc aggtatacgt gggatccgtc caggaggcat acgacgcgac
     1861 gctgatggca tttcgaatcg cagagcacga gtcggtcctc ctccctgtga tggtgaacct
     1921 ggacggtttc ctcctctccc acagcatgca cccgctccag agcgttgaga tcagggactt
     1981 catccccccc atccatctcc cgcatgcgat cgacacggca aacccagccg gttatggaac
     2041 cctgacagga cctgaccagt acttcaggtt ccggtgggac atcgagcgat caatgcgcga
     2101 ctctgtcaac gtcatcaggg agaccgagga ggacttcaga cacagattcg gcagggagta
     2161 ccaccccgtc gaggagtatc gctgtgacga tgcagatgtg gtggtggtcg cgatggggac
     2221 cctcggcaag gaggccgagg ttgccgtcga cctcctccgg aaggaggggg tgaaggccgg
     2281 gagcatgcgt atccgctggt tccgcccgtt cccaaggctc gacctcgcgg atagagacgt
     2341 cgtggtcatc gaccgcgatt actcattcgg cttcggaggg gtgatcgccc ggagccttgc
     2401 tgccaccgag ggcgcaaatc ccttctcggt catcgccggc cttggaggcc aggaggtcac
     2461 ttacgacgat atcgcggggt tcgtgcgcga caggagaccg ggggaggagt tctggttcgg
     2521 ggtgacaccc gatgtatgag atccgcattc actccagggg cgggcaggga ggggtcactg
     2581 cagcacggct cctcgccctt gccgccatcc gtgacgggaa gtacgccacc gcctgcccct
     2641 tctatggcgc cgagcgccgg ggagccccgg tggtctccta cgtgcgcatc gacgacc
//
LOCUS       DAOQVJ010000204         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_46050_length_2658_cov_4.185171, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000204 DAOQVJ010000000
VERSION     DAOQVJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46050_length_2658_cov_4.185171"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..699)
                     /gene="rfbC"
                     /locus_tag="PKL26_05140"
     CDS             complement(109..699)
                     /gene="rfbC"
                     /locus_tag="PKL26_05140"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNS83114.1"
                     /translation="MTSPKFSPIPTSLSGAVLIEPRVFGDDRGFFLESYTRRDMAVLG
                     IPEEFVQDNHSRSAKGVLRGLHYQYPFAQSKLVRVARGAIYDVIVDIRRGSPTFGRHE
                     SFLLSEENHRLLYVPVGFAHGFLALAEGTEILYKAGEYYHPEADAGIRWNDPDLAIPW
                     PVSEHGIAAPLLSDKDARLPLFSDIESPFLYQGASR"
     gene            complement(696..1067)
                     /locus_tag="PKL26_05145"
     CDS             complement(696..1067)
                     /locus_tag="PKL26_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNS83115.1"
                     /translation="MSEKRAYALFLYDMACAVDKILEYTKSLSRQNLLENELVKDAIL
                     RNIQVLGDAAKKVPPEFIASHQEIDWKGIAGLRDIITHQYFRVDWNILWTTIQEDLPP
                     LSSTLHSLLERSLADDNGRSV"
     gene            complement(1060..1353)
                     /locus_tag="PKL26_05150"
     CDS             complement(1060..1353)
                     /locus_tag="PKL26_05150"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNS83116.1"
                     /translation="MKSRLEILSILNNLKQELSPRYHIRRLGIFGSVAKDAHTPTSDV
                     DILIEFDYPISGFSFVHLKELLSSSLEMKVDLVTPGGLHPLLADSIQSDVLYV"
     gene            complement(1497..2378)
                     /gene="rfbA"
                     /locus_tag="PKL26_05155"
     CDS             complement(1497..2378)
                     /gene="rfbA"
                     /locus_tag="PKL26_05155"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNS83117.1"
                     /translation="MKGIILAGGFGTRLYPITKSVSKHLLPVYDKPLIYYPLSVLMLS
                     GIREILIISTARDVPLYRELLGDGSQIGLSFSYAVQEKPGGLAEAFIVGEKFIGTDAV
                     ALVLGDNIFYGQHFSELLRKAASQKHGATIFGYWVRDPRPYGVVEVGPDGQALSIEEK
                     PAKPRSHYAVPGLYFYDNRVIGIAKSISPSARGELEITDLNREYMKKGELFVLVFGRG
                     MAWLDAGTHDSLLEAGNYIETIQKRQGLYVACIEEIAYRQGFIGKDQLRALADGLKNT
                     EYGRYLAELAENESEMQ"
     gene            2477..>2658
                     /locus_tag="PKL26_05160"
     CDS             2477..>2658
                     /locus_tag="PKL26_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83118.1"
                     /translation="MIWMQAYHSSRERQPGRRIIHSKDAIGLYIEGSGEFVYQVRCSF
                     VKENLSIAPAEYSGKT"
BASE COUNT          561 a          714 c          788 g          595 t
ORIGIN      
        1 ggggacctct ctcacttttc cgcattcctg acccgtggat atgggagtga tgcggagagg
       61 gtggttctct ctccaccggg agggaaagga gggactcttc aggaagactc atctgcttgc
      121 cccctggtaa agaaacgggc tctcgatgtc cgaaaaaagg gggaggcgtg cgtccttgtc
      181 ggagagaagg ggtgccgcga tcccatgctc tgagacaggc cagggaattg cgaggtcggg
      241 gtcgttccag cgtatcccgg cgtctgcctc ggggtggtag tattcgcctg ccttgtagag
      301 gatctcggtc ccttccgcga gcgcaaggaa cccgtgggcg aatcccaccg ggacgtagag
      361 cagccggtgg ttctcctcgg agagcaggaa agactcgtgg cgcccgaacg tgggcgagcc
      421 cctcctgatg tccacgatca cgtcgtatat tgcccccctc gccacccgga caagcttgct
      481 ctgggcgaag gggtactggt agtgcagccc ccggagaacc cctttcgccg accgcgagtg
      541 gttgtcctgg acgaactctt ccgggatgcc gagtaccgcc atgtctcttc tcgtgtacga
      601 ctcgaggaag aacccgcggt cgtcgccgaa gacgcggggc tcgatcagca cagcgcccga
      661 aagagaggtc gggatgggag aaaattttgg ggatgtcata cgcttcgtcc gttgtcatct
      721 gccaaggatc tttcaagcag actatggagg gtagaggaga gtggagggag gtcttcctgg
      781 atggtagtcc agagtatgtt ccagtcaacc cgaaaatact ggtgagtaat aatatcacgg
      841 agtcctgcga ttcctttcca gtcaatctcc tggtgagatg cgatgaattc cggcgggacc
      901 ttctttgctg cgtccccgag gacctggata ttacgaagga tcgcgtcctt cacgagttcg
      961 ttctcaagaa gattctgtct tgagagagat ttggtatatt cgagaatttt atcgacggca
     1021 catgccatat cgtataaaaa gagtgcgtag gctctcttct cagacataca gcacatcact
     1081 ctgtatagaa tctgcgagaa gaggatgaag tcctcccgga gtcacgaggt ccactttcat
     1141 ttcgagagat gaagagagga gttccttgag atgcacgaag gagaatccac ttatcgggta
     1201 atcgaactcg atgagaatat ccacgtccga agtcggagtg tgagcgtctt tcgcgaccga
     1261 accgaagatc ccgagccttc ggatatggta acgaggggac agctcctgtt tgagattatt
     1321 cagtattgaa aggatttcca gcctcgattt cattcctgtt tacagttttg cggttcccct
     1381 ataagagtat actccacatc cgaatgagaa tatctccatt atctgccccg ccttttatct
     1441 tcaggccccc gctgcagagc aatcacaggg tgacacagcg gtatctcccc cctgcgttat
     1501 tgcatctcgg actcattctc tgcaagctct gccaggtacc gtccgtactc cgtgttcttc
     1561 agaccgtctg cgagcgcccg aagctggtcc ttccctatga acccctgccg gtacgcgatc
     1621 tcttcgatgc aggccacgta gagcccctgc cgcttctgga tggtctcgat gtagtttccg
     1681 gcctcgagga gcgagtcgtg ggtcccggca tcgagccacg ccattcccct cccgaagacc
     1741 agcacgaaga gctcgccctt cttcatatat tccctgttca ggtcggtaat ctcgagctcc
     1801 cccctggcag agggtgagat agactttgct atcccgatca cccggttgtc gtagaagtac
     1861 agcccgggga ctgcgtagtg ggacctcggt tttgccggtt tctcctcgat cgagagtgcc
     1921 tggccgtctg gtcccacctc gactacgccg tacggcctcg ggtcccggac ccagtacccg
     1981 aatatagtgg caccgtgctt ctgcgaggct gccttcctca gcagttccga gaagtgctgt
     2041 ccgtagaaaa tgttgtcccc gaggacaagc gccacggcat cggtgccgat gaatttttcc
     2101 ccgacgatga aggcctccgc gaggcctccc ggcttctcct ggacggcgta cgagaacgag
     2161 agcccgatct ggctcccgtc ccccaggagt tcccggtaga gggggacatc cctcgccgtg
     2221 gagatgatca ggatctcgcg gatgccggag agcatcagga ccgagagcgg gtaatagata
     2281 aggggcttgt catacaccgg gaggaggtgc tttgagacag acttcgtgat ggggtagagg
     2341 cgggttccga acccgccggc gaggatgatg cctttcatgg gaattcgctg ctattgaggt
     2401 ctggcctgag gagttttata ggttgttgca gggaggggcg aaaaaaaggg actggggcaa
     2461 tgatccctgg tgccgcgtga tctggatgca ggcataccac tcttccaggg agagacaacc
     2521 gggaagaagg attatccatt caaaagacgc gatcgggctg tatatcgagg gaagcgggga
     2581 attcgtgtac caggtcagat gcagttttgt gaaggaaaac ctgtccattg cacctgcaga
     2641 atacagcggg aagacttg
//
LOCUS       DAOQVJ010000205         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_46342_length_2648_cov_3.588122, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000205 DAOQVJ010000000
VERSION     DAOQVJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46342_length_2648_cov_3.588122"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKL26_05165"
     CDS             <1..567
                     /locus_tag="PKL26_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNS83119.1"
                     /translation="QPFQKIKRIGTSSIRRKAQLLMHHPDWRIEPLRGNVDTRIRKLR
                     EGAYDAVVLAEAGLQRLGLCLPGTRLAPEMYVPSPNQGTIAVVSRGGDDAALLAGSLD
                     HQETRRDVTFERVVMEEVGGGCFTPQGVYCRERHIRAEVLSLDGCRRVRREGYLSSAY
                     DAREFGQLLRSDAFDLIQEAYMKLGLKE"
     gene            564..1316
                     /gene="cobA"
                     /locus_tag="PKL26_05170"
     CDS             564..1316
                     /gene="cobA"
                     /locus_tag="PKL26_05170"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNS83120.1"
                     /translation="MTGKVYLVGSGPGGLGLLTLRAREVIDQADVILYDQLPGEEILA
                     TLPERAEKIDCGKFGGRHIREQDDIEALMVAKAREGKKVVRLKGGDPFLFGRGGEELE
                     VMREHGIPVELVSGVTSALAVPGAVGIPLTHRKYASQVTILTGHEEPGKTESGLDWEN
                     LARNPGTLVILMGVKNIPDITRSLVDNGKDPDTPVAVIERGLRSDQRVTVGNLRTIAD
                     IAKSRNVRPPAIIVIGDVVRLYREDDPGLVPL"
     gene            1758..1991
                     /locus_tag="PKL26_05175"
     CDS             1758..1991
                     /locus_tag="PKL26_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83121.1"
                     /translation="MQASSVRLDKTTKEKLDLLRSYPKESYDSIIRRLAESRIDEEPL
                     TDEDRNEIDEALTEIRKGHYYTHDQVKRELGLS"
BASE COUNT          644 a          690 c          801 g          513 t
ORIGIN      
        1 caaccgttcc agaagataaa gaggatcggg acatcgagta taaggaggaa ggcgcagctc
       61 cttatgcacc accctgactg gaggatcgag ccgctccgcg ggaacgtgga cacccgcatc
      121 aggaaactcc gggaaggagc atacgatgcc gtcgtcctgg ccgaggcagg gctccagagg
      181 ctcgggctct gcctcccggg cacgaggctt gctccagaga tgtacgtgcc atccccaaac
      241 caggggacaa ttgccgtggt cagcaggggg ggagacgatg ccgctctcct tgccggctca
      301 ctggaccacc aggaaacccg gagggacgtg actttcgagc gggtggtgat ggaagaggtg
      361 gggggcgggt gtttcacccc gcagggggtg tactgcaggg aaaggcatat cagggcagag
      421 gtactctcgc tcgacgggtg caggagagtg aggagagagg gatacctctc atcggcatac
      481 gatgcccgcg agttcgggca acttctcaga agtgatgcgt ttgacctgat acaggaggca
      541 tacatgaaac tggggctgaa agaatgacgg gaaaggtata tcttgtgggt tccggcccgg
      601 ggggcctcgg gctcctcacc ctgcgggcac gcgaagtcat tgaccaggca gacgtaatcc
      661 tctatgacca gctccccgga gaagagatcc ttgcaaccct cccggagagg gccgagaaga
      721 tcgactgcgg gaagttcggc gggaggcata tccgcgagca ggatgacatc gaggccctga
      781 tggtcgcaaa ggccagggaa ggaaagaagg tcgtgcggtt gaaaggcgga gaccccttcc
      841 tgttcgggag gggcggcgag gagctcgagg tgatgaggga gcacgggatc ccggtcgagc
      901 tcgtctccgg cgtcacgagc gccctcgcgg tcccgggcgc ggtgggaatc ccgctcaccc
      961 accggaaata tgcctcccag gtgaccatcc tcaccggcca cgaggagccg gggaagacgg
     1021 agtccggcct cgactgggag aacctcgcca ggaacccggg cacgcttgtc atcctgatgg
     1081 gtgtgaagaa tatccccgac atcacgcgaa gcctcgtcga taatggcaag gaccccgata
     1141 cacctgttgc ggtcatcgag cggggccttc gctccgatca gcgggtgacc gtcggcaacc
     1201 tccggacgat cgcggacatt gcaaagtcgc ggaatgtgag gcccccggcg atcatcgtca
     1261 ttggggacgt ggtccggctc tacagggaag acgatccggg gcttgtcccg ctgtgaggag
     1321 acaggttagc ttttttaggt gaggaatatc cgagccgcaa gtgaatagtg caacaagata
     1381 ccctgcgtgc gagagaggat ggatatgggc gggctcgatg aaagcgattc tctcgcactg
     1441 ggacctgtct tattgaggct gcgcttctgc gggacgaaag gtccaggcgc atgagagaag
     1501 gattgctgtt ttactattga caggagggcg cgaatccccc cggacctccc cttcctgcga
     1561 taggctgcga ccagtgtgca gggagtataa ccggctttac gttcagatcc gcagcacgga
     1621 ccctatcgcc ttggggggat gccccagtgg cggggcggag ccccaagagc ctcgtcgtat
     1681 gatgagtgcc gttttaccaa aaatcggaac ggttcagaaa tccatagtat tatgtgtaca
     1741 catgcataca cccgtatatg caggcttcct ctgttcgact tgataaaacc acgaaagaga
     1801 aactcgatct cctccggagc tatccgaagg agtcctacga ttcgatcata cgaaggcttg
     1861 ccgaatcgag gatcgacgaa gaacctctca cggacgagga caggaacgag atcgacgagg
     1921 ctctcaccga gatacggaaa ggtcactact acacacacga ccaggtgaaa cgcgaactgg
     1981 gcctctcctg acatgccatg gacgttgatt tattccccac gggcatttca ccagattcgc
     2041 gatttacctg aggcgaccag gaagcgtgtt ctcgaaaagc tcgaaggatt ggcagacaaa
     2101 ccgcttctcc atctccggaa attgaaagac tctcctctct actcattcag gacaggtgac
     2161 tatcgcgttc tctgtgcact ctccacaaag aaactgatca tctgcgtggt gaagatcgga
     2221 catcggaaaa acgtgtataa agatatttga ccggtcaatt cgcattatcg ggtaagcctg
     2281 tccgatcaga aactggattg gaacaattat cgacatggag aagcggttga tgtcggcata
     2341 aaaatatcct tcagagagta actatccacg caaaagtaat attcgcaaaa ttgtatattc
     2401 ccacgtagaa agaatatctt atatatggaa gattccatca aggttagtag ataattggga
     2461 gaagaatcgc agtaccattg ttggccatgt taatcgtctc tcctgcaatc taatccgaaa
     2521 tctctctaga atatacgaga acggcgttga aggagagcca ggaattcatt cgggagacct
     2581 gccgggttat cccggcacac gaccttgtgg ctgaagcgtt cgacatggcg tgttcctccc
     2641 ggatcaca
//
LOCUS       DAOQVJ010000206         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_46673_length_2636_cov_3.842309, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000206 DAOQVJ010000000
VERSION     DAOQVJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46673_length_2636_cov_3.842309"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1417)
                     /locus_tag="PKL26_05180"
     CDS             complement(77..1417)
                     /locus_tag="PKL26_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS83122.1"
                     /translation="MFERILVPTDFSWYAQRTVRYATEIPGATGVILLHIAEDAGECM
                     TWPSLMEARNAPCTPANALAYAGDALEKAGLSVTYEVAPPKDAGTHAAILACAEQHKA
                     DLILIGGKGRGFFKDMLLGSVSRRVIEEAKTHVLVTHFQEQPGHGNEAPDVPVATGRP
                     LFSRILVPVDFSRPTYETIEFLSDVGGYDEIILMHVIASVEDRHDLQKKLQHSMQALS
                     YLQRDLALGGGKIIPLIRFGDTVGEICRMADEERATLILISRFGASDYMKDGSIGSIA
                     EEVTKRARVPVLVRYPGSSLDVQVREVGAAEFPRAEEVWAHYRQQRADPSTDRIFGVF
                     VQGDPVSLARCRRFPDGLEVDGVFTLEGFRRRGYARGAVSLLVRECGAEPLHTFSTAD
                     MIEFFRSFGFNEIPEKEMPGSIRERYSFALGDLAGADLYPMSRSAKGASEAPAG"
     gene            complement(2201..2587)
                     /locus_tag="PKL26_05185"
     CDS             complement(2201..2587)
                     /locus_tag="PKL26_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNS83123.1"
                     /translation="MAGTTRYKCKVCGYIYSPVRGEPHNGIPAGTDFEDLPDSYVCPI
                     CGMEGKGKIGKWGFEEWVPTKWICSVCGYVYDQRRGEPHRGIKPGTAFEDLPDDYTCP
                     VCNLDPKISREYGKVFKQGFDPLDLP"
BASE COUNT          553 a          775 c          723 g          585 t
ORIGIN      
        1 cagagcgctc cccgttctcc ttcacgggga tggttcgagt cgggattttc catcctgagc
       61 gcgcaacttt cccatcctat cccgcagggg cttctgacgc ccccttggca ctcctgctca
      121 tggggtacag gtccgcgcct gccaggtccc ccagggcaaa actgtagcgt tcccggatgg
      181 agccgggcat ctccttttcc gggatctcat taaacccaaa agacctgaag aactcgatca
      241 tatcagcagt ggagaaggta tgaaggggct cggctccgca ctcgcgcacc aggagcgaga
      301 ctgcgcccct tgcatagccc ctgcggcgga acccttccag ggtaaacacg ccgtccactt
      361 ccaggccgtc agggaaccgc ctgcatcggg cgagggagac aggatccccc tggacaaata
      421 cgccgaatat gcgatcggtg gagggatctg cacgctgctg ccggtaatgg gcccatactt
      481 cctctgcacg tggaaattcg gctgcgccga cctcccggac ctgcacatcg agggacgagc
      541 cggggtaacg gacgaggaca ggaacgcggg ccctctttgt cacctcttct gcgatgctgc
      601 cgatggatcc gtccttcatg tagtctgatg ccccgaacct tgagatgagg atcagggtag
      661 cgcgctcctc gtctgccatc ctgcagatct caccgacagt atccccgaaa cggatgaggg
      721 ggatgatctt cccgcctccg agcgcaagat cccgctgcag atatgagagc gcctgcatgg
      781 agtgctggag cttcttctgc aggtcatgcc ggtcctcgac cgaggcgatg acgtgcatga
      841 ggatgatctc gtcatacccg cccacgtcgc tcaggaattc gatggtctcg taagttggcc
      901 ttgagaagtc cacggggaca aggatccgcg agaagagcgg tcttcccgtg gcaacaggca
      961 catccggggc ctcgttcccg tgaccgggct gctcctggaa atgggtgacg aggacgtggg
     1021 tctttgcttc ctcgattacc ctcctggaga cacttcccag gagcatgtcc ttaaaaaaac
     1081 ccctaccttt cccaccgatc agaatgaggt ctgccttgtg ctgctccgca cacgcgagga
     1141 tagcggcatg ggtgcctgca tcctttgggg gcgccacttc gtaggtcacg gacaacccgg
     1201 ccttctcgag cgcatctccg gcataggcaa gtgcattggc gggtgtgcac ggtgcattcc
     1261 tcgcttccat gagtgagggc cacgtcatgc actctcctgc atcttcggcg atgtgcagga
     1321 gtataacacc agtcgctccc ggaatctcgg ttgcataccg gacggttcgc tgggcatacc
     1381 aggagaagtc ggtcgggaca aggattcgct caaacatgtt gaaacacagc cggatgagtt
     1441 ggtcctgtaa gaatgttggc ccggggggta ttaatctgta tatcccgggg ttcacccgtg
     1501 ccaaccggac agtattcttg tatccatcca caagccggtt ttttcaacca tatatcatat
     1561 gatgcgatac acaaaggggg agtccttctc cgttcctgcc agacagggca tctccgggag
     1621 ccgcggcgcc cccccagtcc cggaacaact cctcattcct gtgaaatgct gtgaatgata
     1681 tccgtcattg cccctccctt gcagttccgg ccactaccta ttgcgggatg tgcttccatg
     1741 ggagccattt tcaatttaaa aagcctcctc gctgttttgt gtggggagac tgaatcgggg
     1801 ttgttatctg catatcaatt cagtcactcc aaccgcagcc gtgctttttc acagaactcc
     1861 tcccagatta ctcaggagtg gccctaaatc tgaaggtgac cgcaggtcgc caagggtcga
     1921 gggcggaagc cccgcggtat gttcgggggc ggcagcgtgc atcgcacatg ggggtgtagg
     1981 ggcagacagc ccccacaaaa tgtgtccggt tcaccaattc tacccacacc agatagcgaa
     2041 gaaccattaa aaaaaaacta catgattctc ttcgaggcac acgcaataca gatgaaaatc
     2101 gcgggtgcag ttttcattcg aaaaaagtga tgctcatatg ccagggtacc ggtttttcaa
     2161 ggattactgg cgcttttgga aaaaatgatt cagagatgtt tcacggaagg tccagcgggt
     2221 cgaacccctg cttgaacacc tttccgtatt ccctggaaat ctttgggtca aggttgcaca
     2281 ccgggcaggt gtagtcatcc gggaggtcct cgaaggcagt tcccggcttg ataccgcggt
     2341 ggggttcacc ccgcctctgg tcatatacat acccacagac agagcagatc cacttggtgg
     2401 gaacccactc ctcgaatccc cactttccga tcttcccctt cccctccatt ccgcagatgg
     2461 ggcagacgta gctgtccggt aagtcttcaa aatctgtccc tgcagggatg ccgttgtgag
     2521 gttctccccg cacgggggag taaatgtagc cgcagacctt gcatttatag cgtgttgttc
     2581 ccgccatgat cacacatctt cccttataat gcaatgtgat gccctggcag gattta
//
LOCUS       DAOQVJ010000207         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_46923_length_2627_cov_5.160575, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000207 DAOQVJ010000000
VERSION     DAOQVJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46923_length_2627_cov_5.160575"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKL26_05190"
     CDS             <1..613
                     /locus_tag="PKL26_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNS83124.1"
                     /translation="VMRPDVNEVVGVVDLGESLGVKDYQVFFPVPTGRARGSDIMNPL
                     VYEDLIRGVLERYRDAPLHIRPTCAPQFRRIADELGIENPTWGRGCIAGITYCRIYAN
                     GDVTPCPYLPISAGNVREQSFAEIWHHSEVFSTLRDISRLTGKCGRCHYRSICGGCRA
                     RAYQGGRTVSTGWCDGLALPAALQGEMCGEDPWCPYEPGEFAE"
     gene            610..1122
                     /locus_tag="PKL26_05195"
     CDS             610..1122
                     /locus_tag="PKL26_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNS83125.1"
                     /translation="MSGRFIPDKLDIAILQALQEEIPLVSRPWKEISGKIGISEQELL
                     ARLNRIKNAGILKAVSPIIESSECGRGAATLVAMHVPSGRLQEVAAIISGYPEVSHNF
                     RREHYYQVWFTLSGRDEEHIRVLVRDILERTGIGEDDILDLPTVNKLKVDVRFSFNGT
                     KATGGNSGSC"
     gene            1109..1597
                     /locus_tag="PKL26_05200"
     CDS             1109..1597
                     /locus_tag="PKL26_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNS83126.1"
                     /translation="MDPVDIRLLALLEEGLPFVSHPYVEIGRQLQMTESEVLERLKWL
                     REEGVIRKFRARINQRKVGITANALVAWKRGESGNGDVGSLLASYPCVTHCYERLPVP
                     GRWEYIFYTVHHGYSREEVLEEVLALSSRIGVADYLVLFSTEEFKRVPNVRIRENGGI
                     MP"
     gene            1594..>2627
                     /locus_tag="PKL26_05205"
     CDS             1594..>2627
                     /locus_tag="PKL26_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS83127.1"
                     /translation="MNRITQCIHGRGTVSAVMKHRDRPSGEVPSRYLAFAGMRRPVVF
                     WNITNSCNLGCEHCYSRSGPGLRADDELDTDEGFDLIDDLAGMGVPLILFSGGEPLMR
                     ADIWDLAEHAGEKGIKTALSTNGTLITKEVAEKIRNSGIKYAGISLDGARSATHDRFR
                     NSPGAFDRTIAAFARCREAGVRCGVRITLTKENSHELADLIDLSLAIGASRFCLYWMV
                     PCGRGTDSYHRLQVGSNVVEDALGLLYRRAQEIDPSMMEFLTVDAPQDCIHLIQSMER
                     DGSPDLADAQALLASLNGGCSAGDRVANIDPSGNVYPCQFARSPAFLVGNIRERPFSV
                     MWAEETHPVL"
BASE COUNT          570 a          719 c          814 g          524 t
ORIGIN      
        1 ggtcatgcgc ccagacgtga acgaggtcgt aggagtggtc gacctcgggg aatcgctcgg
       61 tgtgaaggat taccaggtct tcttccctgt ccccaccggc cgggcacggg gaagcgatat
      121 catgaaccct ctcgtgtacg aagacctgat caggggtgtg ctggagagat acagggatgc
      181 cccgctgcat atccgcccga catgtgcccc gcagttccga cgcatcgcag atgaactcgg
      241 gatagagaac cccacatggg ggagaggctg catcgcgggc atcacctatt gccggattta
      301 cgcaaacggt gacgtgaccc cctgcccgta tctcccgatc agtgcaggaa acgtgagaga
      361 gcaatctttt gctgaaatat ggcatcattc agaggttttt tcgacactcc gtgacatcag
      421 ccggctcacg ggcaaatgcg ggaggtgtca ttacaggtcg atatgcgggg ggtgcagggc
      481 cagggcatac cagggaggca ggaccgtttc cacgggatgg tgcgacggcc ttgccctccc
      541 cgcggcgctc cagggagaga tgtgtggcga agacccgtgg tgcccatatg aaccggggga
      601 gttcgcggaa tgagtgggag attcataccc gataaattgg acatcgcaat cctgcaggcc
      661 ctccaggagg aaatcccact ggtctcccgc ccatggaagg agatctccgg gaagatcggc
      721 atttccgagc aggaactcct cgcccggctg aaccgcatca agaatgccgg catcctcaag
      781 gcggtctccc ccatcataga atccagtgaa tgcggacgtg gtgccgcaac tctcgttgcc
      841 atgcatgtcc cctccggcag gctgcaggag gttgccgcca tcatcagcgg gtaccccgag
      901 gtctcccaca atttccggag agagcattac taccaggtct ggtttaccct ctccgggaga
      961 gacgaggagc atatcagggt ccttgtcagg gatatccttg aacgcacggg aataggagaa
     1021 gatgatattc tggatctccc tacggtgaac aaactgaagg tggatgtccg tttctctttt
     1081 aacgggacca aagcgacggg aggaaacagt ggatcctgtt gacatccggc tgcttgccct
     1141 cctggaagaa ggcctccctt tcgtcagcca tccctacgtc gagatcggaa gacagttgca
     1201 gatgaccgag agcgaagttt tggagaggct caaatggctc agggaagagg gagtgatacg
     1261 gaagttccgg gcccgcatca accagagaaa ggtaggcatc acggcaaacg ccctcgtggc
     1321 gtggaaacgc ggggaatccg gcaatggtga tgtgggttcc cttctggcat cgtatccttg
     1381 cgtgacccat tgctacgaac gactcccggt ccccggcagg tgggagtaca ttttctacac
     1441 cgtgcaccac ggatacagcc gcgaggaagt tcttgaagag gttttggctc tttcgtcccg
     1501 tatcggggtg gctgactacc tcgtgctgtt cagcaccgag gaatttaaaa gggtcccgaa
     1561 tgtccggatc cgggagaacg gaggcatcat gccatgaaca ggatcaccca gtgcatccac
     1621 gggaggggca cggtgagtgc cgtgatgaag cacagggaca ggccttcagg cgaagtgccg
     1681 agcaggtacc tcgcatttgc gggtatgcga aggccggtcg tgttctggaa catcacgaac
     1741 agctgcaatc tcgggtgtga gcactgctac agccgatcgg gccctgggtt gcgggctgat
     1801 gatgaactgg acacggatga aggattcgat ctcatcgacg accttgcagg gatgggagtg
     1861 ccccttattc tcttttcggg aggggagccc ctgatgaggg cggatatctg ggacctcgcg
     1921 gagcacgcgg gtgagaaggg aataaaaact gcgctcagca ccaacgggac tcttatcaca
     1981 aaggaagtag cggagaagat caggaattcc gggataaaat acgcggggat ctcgcttgat
     2041 ggtgcacggt ccgcgaccca tgaccggttc aggaactccc ccggcgcatt cgatcggact
     2101 atcgcggctt ttgcacgctg cagggaggct ggtgtccggt gcggggtgag aatcacgctc
     2161 acgaaagaga actcccacga gcttgctgac ctgatcgatc tctcccttgc catcggggcg
     2221 tcccgtttct gcctttactg gatggtgcca tgcggaaggg ggaccgattc gtatcaccgg
     2281 ctccaggtgg gaagtaacgt tgtggaggat gccctcggac ttctctaccg gagagcacag
     2341 gagatcgatc cctccatgat ggaattcctt accgtcgatg ctcctcagga ctgtattcat
     2401 ctcatccagt caatggagcg agacggatcg cctgaccttg ccgatgccca ggccctgctc
     2461 gcttcactga atggcgggtg cagcgcaggc gacagggtcg ccaatatcga cccttctgga
     2521 aacgtgtacc cctgccagtt cgcacgatcg cccgcatttt tggttggaaa catccgggaa
     2581 cggccgttct ccgtgatgtg ggcggaagag acccacccgg tcctcca
//
LOCUS       DAOQVJ010000208         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_46954_length_2626_cov_5.014002, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000208 DAOQVJ010000000
VERSION     DAOQVJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46954_length_2626_cov_5.014002"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKL26_05210"
     CDS             <1..377
                     /locus_tag="PKL26_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNS83128.1"
                     /translation="QIPLFYQVLVSMIFVAVIPVILLSVVSMGGTASIVATIGTPATV
                     LLLTIGTVLVVLLWSYFVAHRVTRPIVELSVVATRISRGYLPEKEMEVQSHDEIGELI
                     AAFNKMVNTYRILDTLAKEEPE"
     gene            415..762
                     /locus_tag="PKL26_05215"
     CDS             415..762
                     /locus_tag="PKL26_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS83129.1"
                     /translation="MLKNELKRLFSLEGVKMVLVIDPEGRVHDEMGASNIEGEQLAAV
                     VSFVMAESRAMATRLGQEQISMVFVEFRELALVSVPLQEAYFLVVITHAGANIGQISY
                     ELKKYQDISSGYT"
     gene            759..1634
                     /locus_tag="PKL26_05220"
     CDS             759..1634
                     /locus_tag="PKL26_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83130.1"
                     /translation="MTGMLPAGFPVGEMQAPFKWMLIHAARFQGMIRVQAPDGEGHVL
                     VRKGTPMGCSFRRGSEDLKGEAAYQHILSFPVIDLSIHRYTTEEMEEAIRLLQASGSV
                     CGGGGAGPAYGSGGQPGETGSATGEERSPLPPAEADAPTAPVEKSRHTAAGTRETSPG
                     SLEEAPMVLPSGLDVENLAHLLLGRMLRLQGVQAVSIFGRGKSVLSIGDVELDSLVVL
                     AEDMLHAAKDISSVMRTGAFVHLTLQIPAGKVIIAPYFNEYICILTSPGVNLGQIRKI
                     LKEIPETGSPGRTVS"
     gene            1634..2293
                     /locus_tag="PKL26_05225"
     CDS             1634..2293
                     /locus_tag="PKL26_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS83131.1"
                     /translation="MYRILIVDDNPAITDILARMVRHEGLEAIEAHSGEEALAILGQD
                     HPDLIFLDILMEPMDGWETLDRIKRDKRTADIPVMMITAKSLDPAEVQAHTAHFEDYI
                     QKPVTRRELCRVIRQYFRKESEIIDEVEKARLSGMDAGLVDEFRRLSRDIEVHQRLLL
                     LLWNVYRTREDDDPQRPDLLEAIGRMEAAQNAMRSRHHEIRTRIRSQGKGCGSEGETG
                     G"
     gene            2330..>2626
                     /locus_tag="PKL26_05230"
     CDS             2330..>2626
                     /locus_tag="PKL26_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HNS83132.1"
                     /translation="MKNLLLGIGNPLRKDDGAGNYVAKSFRKAGWQVIDCGTAPENFS
                     GTVRRHHPDLLVLVDAADMGLAPGEFAIIPKEKIRDVGIGTHQLPLDIFIDFLGD"
BASE COUNT          576 a          736 c          801 g          513 t
ORIGIN      
        1 tgcagatacc cctcttttac caggtgctgg tcagcatgat cttcgtggca gtgataccgg
       61 taatcctcct ctcggtggtc tccatggggg gcactgccag catcgtggcc accattggca
      121 ctcctgccac cgtccttctc ctcaccatag gcaccgttct tgtggtcctc ctgtggagct
      181 acttcgtggc gcaccgtgtc acaaggccga tcgtagaact ttcagttgtc gccaccagga
      241 tcagcagggg atatctgccg gaaaaggaga tggaggtcca atcccacgat gagatcgggg
      301 aactcattgc cgcattcaat aagatggtaa atacctaccg gatactggat acgctggcga
      361 aggaagagcc ggagtgaact tctgtcaacc aggtgcaagg agaaaacgac tgcaatgctg
      421 aagaacgaac tcaagcgcct cttctccctc gaaggagtga aaatggtgct cgtcatcgac
      481 cccgaaggca gggtacatga cgagatgggg gcatccaata tcgagggtga acagcttgca
      541 gcggtggtgt ccttcgtgat ggccgaatcc agggcgatgg cgacgcgcct tggccaggaa
      601 cagatctcca tggtgttcgt ggagttccgg gaacttgccc ttgtatccgt tcccctccag
      661 gaggcatatt tccttgtcgt gattacccat gcaggggcga atatcggcca gatcagctat
      721 gaattgaaga agtaccagga tatctcgagt gggtacacat gaccggaatg ctacccgccg
      781 ggtttccggt cggagagatg caggcgccgt tcaagtggat gctcatccac gccgcacgct
      841 tccaggggat gatccgcgtg caggcacctg atggggaggg gcatgtcctt gtcagaaagg
      901 gcaccccgat gggctgttcc ttccggaggg ggagcgagga tctgaaagga gaggctgcat
      961 accagcacat actcagcttt cccgtcatcg acctctccat ccacaggtat accaccgaag
     1021 agatggaaga ggccattcgg ctgctccagg catctggttc ggtttgtgga gggggaggcg
     1081 cgggacctgc atacggatcc ggtggtcaac ccggggaaac aggatctgcg acaggggaag
     1141 agcgttctcc cctgccccct gcagaagctg atgcgccgac tgcgccggtc gaaaagagca
     1201 ggcataccgc cgccggcacg agggagacct ctcccgggtc tctcgaggag gccccaatgg
     1261 tcctccccag cggcctggat gtcgagaacc tcgcccacct gctcctgggg cgcatgctca
     1321 ggctccaggg ggtccaggcg gtctcgatct tcggcagggg caagtcggtg ctctccatag
     1381 gggacgtgga actcgactcc ctggtggtcc tcgcagagga catgctccat gctgcaaaag
     1441 atatcagctc tgtcatgcgt accggcgcat tcgtccacct gaccctgcag atacctgccg
     1501 ggaaggtaat catcgcaccc tatttcaacg agtatatctg catcctcacc agtcccgggg
     1561 tcaacctcgg ccagatacga aagatcttaa aagagatccc ggaaaccggg agcccgggga
     1621 ggaccgtttc ctgatgtacc gtattctcat cgtggacgac aacccggcca ttaccgatat
     1681 ccttgcaagg atggtgaggc acgaggggtt ggaggcgatc gaggcccatt ccggagagga
     1741 ggcgcttgcc atccttggcc aggatcaccc tgacctcatc ttcctcgaca tcctgatgga
     1801 accaatggac gggtgggaga ctcttgacag gatcaagcgg gacaaacgga ctgccgatat
     1861 cccggtgatg atgatcaccg caaaaagcct cgaccccgca gaggtccagg ctcacaccgc
     1921 gcacttcgag gactatatcc agaagcctgt caccaggcgg gaactctgca gggttatcag
     1981 gcagtatttc aggaaagagt cggagattat cgacgaggtg gagaaggccc gcctgtccgg
     2041 tatggatgcc ggtctcgtcg acgagttccg gagactctcg agggatatag aagttcacca
     2101 gagacttttg ctcctgctct ggaacgttta ccggacaagg gaagacgacg atccgcagag
     2161 gcctgatctc ctcgaggcca tcgggcgcat ggaggcggct cagaatgcca tgaggagtcg
     2221 tcatcatgag atcaggaccc ggatcaggtc ccaggggaaa gggtgcggaa gcgaaggcga
     2281 gaccggtggc taggggcatt ggtcacagcc gcatcgtgga cctgattgca tgaagaacct
     2341 cctccttggc atcggcaacc ctctccggaa ggatgacggt gcagggaatt acgtggcaaa
     2401 atctttccgg aaagcgggct ggcaagtgat cgactgcggg actgcccccg aaaatttctc
     2461 gggaacagtg aggcgccacc acccggatct tctcgtcctc gtggacgccg cggacatggg
     2521 tctcgccccg ggggagtttg cgatcatccc gaaggagaag atcagggatg tggggattgg
     2581 tacccaccag ctcccgctcg atatcttcat cgactttctc ggggat
//
LOCUS       DAOQVJ010000209         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_47128_length_2620_cov_3.925926, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000209 DAOQVJ010000000
VERSION     DAOQVJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47128_length_2620_cov_3.925926"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PKL26_05235"
     CDS             complement(<1..1547)
                     /locus_tag="PKL26_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNS83133.1"
                     /translation="MNDTNNLVYRMGPGCDLGDVEVGKTYIGRVQGFANFGTFVQLND
                     RVKGLVHKSNARTTHRERESILVRVTNIRSNGNIDLEEVTIPVYTVEMVEKKSTAVRL
                     SELGGKVGRTVRIEGEIAQVKQTSGPTIFTIVDETGTENAAAFVEAGVRAYPEAELGD
                     IVRIVGEVMMRQNQLQIEVESLSILAGEEAEAVRERIDRALESRAEPPDIPFLVASDV
                     LEKLRPEMRKVAKVIRKAVFSSQPILLRHHADADGICSGVAIEQAVVSLIRESGGDFD
                     ADYFLFKRAPSKAPFYEIEDITRDLDFATKDHVRYGQKMPLVILTDNGSTEEDLPSMK
                     IAQVYDLPIVVIDHHHPDAIVDQYIAGHVNPYHVGGDFGITAGMLGTEVARMINPSVE
                     NLIRHLPAVAAVGDRSEAPERARYLSLVQDAYSEQDCKDIALALDYQQFWLRFNDGRE
                     LVKDLLNLAGNTSRHKKLVALQVEGANLAIQDQMSACMPHVVHRTLRNGAELFLLDVE
                     IHAHKFTS"
     gene            complement(1644..2612)
                     /gene="rnz"
                     /locus_tag="PKL26_05240"
     CDS             complement(1644..2612)
                     /gene="rnz"
                     /locus_tag="PKL26_05240"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNS83134.1"
                     /translation="MSGETLHVYFLGTAAALPTPMRNPACIMIKRGRDTLLFDCGEGA
                     QQQMMRARTGFLVDAVFITHWHADHFLGLFGLAQTLSFMGRTEPLTIYGPEWVHEVAA
                     SVRALSRYNVKFPVNSVEIRPGSVVRFSGYTVRAFETAHGMAGLGYVLEEDERPGRFN
                     RQRAIEIGVPPGPLFGRLQRGETVRIVKDGGEREVKPGDVMGQPRPGRKIVYTGDTRP
                     IHTRIRELAHDPDLLIHDATYDDQAIDRAREVLHSTAGEAGEAAEFLNARRLALVHIS
                     SRYTTTASHKKDASARFGGHVLVPSDLEMLEIPYSDSGEATAGSGD"
BASE COUNT          448 a          827 c          805 g          540 t
ORIGIN      
        1 gaggtgaact tgtgtgcatg gatctcgaca tcgagaagga agagctctgc cccgttccga
       61 agcgtccggt gcacgacatg cggcatgcag gcactcatct ggtcctggat ggcaaggttt
      121 gctccctcga cctggagggc cacgagtttc ttgtgccgcg aggtgttccc tgcaaggttc
      181 aggaggtctt ttacaagctc ccggccatcg ttgaaccgga gccagaactg ctggtaatca
      241 agcgcgagcg cgatgtcctt gcagtcctgc tccgagtacg cgtcctggac aagtgagagg
      301 tagcgggcac gctccggcgc ctcgctcctg tcgccgaccg ccgccacggc cgggaggtgc
      361 cggataaggt tctcgaccga cgggttgatc atccgcgcca cctcggttcc gagcatcccg
      421 gccgtgatcc cgaagtcgcc ccccacatgg taggggttga cgtgcccggc gatgtactgg
      481 tccacgattg catcggggtg gtggtggtcg atcacgacaa tgggaagatc gtatacctgc
      541 gcgatcttca tcgatgggag gtcctcctcg gtggagccgt tgtcggtgag gatcacgagc
      601 ggcatcttct gcccgtaccg gacgtggtcc ttcgtcgcga agtcgaggtc gcgggtgatg
      661 tcctctatct cgtagaacgg cgctttcgag ggggcacgct tgaacaggaa gtaatcggcg
      721 tcgaagtccc cgcccgactc gcgtatgagg gagacgaccg cctgctcgat ggcgactccc
      781 gagcagatcc cgtccgcgtc cgcatggtgc cggaggagga ttggctggga ggagaagacc
      841 gctttcctga tgacctttgc caccttccgc atctccggcc gcagcttctc gagcacatcg
      901 gatgcgacaa ggaacgggat gtcgggtggc tcggcacgtg attcgagcgc cctgtcgatc
      961 cgctccctga cggcctcggc ctcctcgccg gcgaggatcg agaggctctc cacctcgatc
     1021 tggagctggt tctgccgcat catcacctcg ccgacgatcc tgacgatgtc cccgagttca
     1081 gcctcgggat aggcccgaac gcccgcttcg acgaaagccg ccgcgttctc ggtcccggtc
     1141 tcgtccacga tggtgaagat ggtggggccg ctcgtctgct tgacctgcgc gatctccccc
     1201 tcaatgcgga ctgttcttcc cacctttccc ccgagctccg agagacggac cgcggtcgac
     1261 ttcttctcca ccatctcgac ggtgtagacc gggatcgtga cctcctcgag atcgatgttc
     1321 ccgttgctcc tgatgtttgt caccctgacg aggatgctct cccgttcccg gtgggtggtg
     1381 cgtgcattgc tcttgtgtac cagtcccttc accctgtcgt tcaactgtac aaatgtgccg
     1441 aaatttgcaa acccctggac gcggcctata tacgtcttcc cgacttccac gtcgccgagg
     1501 tcgcaccccg gccccattcg gtataccaga ttattcgtgt cattcatagg tatgccagat
     1561 cgaggcagga tcttcttccc ccctcccgat ccgttctcct ttgagtatat caataggcgc
     1621 gggattctta tgtaagtatg gaattaatcc ccggagccag cggttgcttc ccctgaatcg
     1681 gagtagggaa tctccagcat ctcgagatcg gaagggacca gcacatggcc gccaaaccgt
     1741 gcggatgcat ccttcttgtg gctggcagtg gtggtatacc tcgagctgat atggacaagc
     1801 gcaagccggc gggcattgag gaactccgcc gcctctcccg cttctcccgc agtagagtga
     1861 agcacctccc gcgcccggtc gatggcctgg tcgtcgtagg tggcatcgtg gataaggagg
     1921 tccggatcgt gcgcgagctc cctgatccgg gtgtggattg gccgggtgtc accggtatag
     1981 acgatctttc gcccaggccg cggctgaccc atgacatcgc cgggcttcac ttctctctca
     2041 cccccatcct tcacaatccg gacggtctcc cctcgttgca gccggccaaa gaggggcccg
     2101 gggggaaccc cgatctcgat ggccctctgt cggttgaacc gccctggcct ctcgtcctcc
     2161 tcgaggacgt agccgagccc cgccatgccg tgggctgtct cgaatgcccg gaccgtgtag
     2221 cctgagaacc gcacgacgga accgggccgg atttcgacag aattgacagg gaatttcacg
     2281 ttgtacctcg agagcgcccg gacagacgct gcgacctcgt ggacccattc ggggccatag
     2341 atggtcaggg gctcggtcct gcccatgaac gagagggtct gggcgagccc gaacaacccg
     2401 aggaaatggt ccgcgtgcca gtgggtgatg aagactgcgt ccaccaggaa accggtcctg
     2461 gccctcatca tctgctgctg ggcaccctcg ccgcagtcga agaggagggt gtccctgccc
     2521 cgcttgatca tgatgcaggc ggggttgcgc atgggggtgg ggagcgccgc ggccgtcccg
     2581 aggaagtaga cgtggagcgt ctcgccgctc atgcaaacca
//
LOCUS       DAOQVJ010000210         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_47247_length_2616_cov_4.434596, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000210 DAOQVJ010000000
VERSION     DAOQVJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47247_length_2616_cov_4.434596"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PKL26_05245"
     CDS             complement(<1..1059)
                     /locus_tag="PKL26_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS83135.1"
                     /translation="MAFSDLHLNTMDRYKPAILLLLVAVSVILEAVVHHAMGIEVVYS
                     HFFYIPVVLGAIWYGKKGVLVAVFLAAVYAGDYYLINGVVHTDAVMRALMFVVVAFVI
                     GMVMDYIHREQEQVMTQMANAALGGGTSGGFRGNLGELKAKLRSSVNVKRLKEEGDIG
                     GLIGALGHRDAAIQYEAAEALGVLKEPSAVTPLMTALTGDRYSGVRWKAAEALARIGG
                     PAVEPLVGALGHSDEDVRWKAAIALGEIGDARAIPPLVALLEDQDRFVQSRAAYSLGQ
                     FGNAALSPLLLVLEAGSPAARRGAAIALGKVGDPAVMEPLLSALSDPDEGVRSAVIET
                     LAGLGEGVHSRVLAHIRGL"
     gene            complement(1144..2325)
                     /locus_tag="PKL26_05250"
     CDS             complement(1144..2325)
                     /locus_tag="PKL26_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HNS83136.1"
                     /translation="MILSAEEEAILAGEEGETRARMLEILLALGKVYGAERLIPIRSA
                     QVSGASYKTIGRFGLDWLQQLDTKVSVPTVLNPVGMDRSRWREMGITPEFARVQEQVL
                     DAYRRLGVTMECTCTPYYLYMTRFGDHLAWSESSAVAYANSVIGARTNREGGPSALAA
                     AIIGKTPEYGLHLVGARKPQVVIRVEEGDRLVDAAHFGALGYLAGRLVGSRIPLLEGI
                     RPKKDLLKALGAAMAASGAVALFHVKGITPEARVFSYPTEGLEVIEIGYGEISALMKE
                     IPVDAVALGCPHCSPEELSRIAGLLAGKKVGIPVFVFAARDVAVHHPAEVAAIEESGA
                     HVFADTCMVVSPAMERFGAIMVNSGKAFAYIPNMCGAAVRLGSTEECIEVATRPAAGA
                     S"
     gene            complement(2322..>2616)
                     /locus_tag="PKL26_05255"
     CDS             complement(2322..>2616)
                     /locus_tag="PKL26_05255"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNS83137.1"
                     /translation="QGDAKNAIMAAFAAHTSLKHVIVVDEDIDPSDPAEVEFALATRV
                     RGDRDILVVTGARGSSLDPCRVGDGTNVKVGVDATMVLGQEAEFLRVGWQEGQ"
BASE COUNT          469 a          901 c          785 g          461 t
ORIGIN      
        1 gagtccgcgg atatgagcga ggacgcgtga gtgcacaccc tccccgaggc ctgcgagggt
       61 ctcgatcacc gccgagcgca ccccttcgtc aggatccgag agcgcggaga ggagcggttc
      121 cattaccgcg gggtctccga cctttccaag cgcaattgcg gctcccctcc tggcggccgg
      181 gctcccggcc tccagcacga gcagcagggg agagagcgcg gcgttgccga actgccccag
      241 cgagtacgcc gccctgctct ggacaaaccg gtcctggtct tcgaggagcg cgacgagcgg
      301 ggggattgca cgggcgtcgc caatctcgcc aagggcaatc gctgccttcc accgcacgtc
      361 ctcgtcggag tgcccgagtg cgccaacgag aggctccacg gcaggtccgc cgatccgtgc
      421 aagggcttcg gccgctttcc accgcacccc tgagtagcgg tcgccggtca gggctgtcat
      481 caggggagtg accgcggacg gctccttcag cacacccagg gcctcggccg cttcatactg
      541 gatcgcggca tcccgatggc cgagggcacc gatgagcccc ccgatgtccc cctcctcttt
      601 gagccgcttc acgttgaccg aagaccggag ctttgccttc agttccccga ggtttccccg
      661 gaatcccccg gaagtgcccc ctcccagggc ggcgttggcc atctgggtca ttacctgctc
      721 ctgctccctg tggatatagt ccatcaccat cccgatgacg aacgcaacca ctacgaacat
      781 cagggctctc attaccgcgt cggtatgcac gactccgttg ataagatagt aatcgccggc
      841 gtagaccgcc gcgaggaaaa ccgcgaccag gacacccttc ttcccgtacc agatcgcccc
      901 gaggacgaca gggatgtaga agaagtggga gtagaccacc tctatcccca tcgcatggtg
      961 cacgaccgcc tcgaggatga cggagaccgc gaccagcagg aggaggatgg ccggtttgta
     1021 acggtccatc gtgttcaggt gcaggtccga gaatgccatt cagttgggta tttgcataaa
     1081 atcgttgata aatccaccgg gaacaaagag atgtgcccgg tccccggcgc gaatgcgccg
     1141 tgttcaggag gcccctgcag ctggccgcgt cgccacttcg atgcactcct cggtgctccc
     1201 gagccttacc gcagcaccgc acatgttcgg gatgtaggca aacgccttcc ccgagttcac
     1261 catgattgcg ccgaaccgct ccatcgcagg agagaccacc atgcaggtgt ctgcaaagac
     1321 atgcgccccg ctctcctcga tcgccgcgac ctcggcaggg tggtgcaccg cgacatccct
     1381 tgccgcaaag acaaagacag ggatcccaac ctttttgccg gccaggagtc ctgcgatcct
     1441 ggagagctct tccggcgagc agtgggggca tccaagggcg actgcatcga cggggatctc
     1501 cttcatgagc gcggatatct ccccataccc gatctcgatg acctccagcc cttctgtggg
     1561 ataggagaag acccgggctt cgggggtgat ccctttcacg tggaagaggg cgactgcgcc
     1621 cgaggctgcc atggcggcgc cgagcgcctt caggaggtcc tttttcggcc ggattccctc
     1681 cagcaggggg atacgactcc ccacgagcct gcctgcaagg tatccgagtg ccccgaagtg
     1741 tgccgcgtca acgaggcggt caccctcctc cacgcggatg accacctggg gcttccttgc
     1801 cccgacaagg tggagcccgt actcgggcgt cttgccgatg atcgccgcag cgagtgcgct
     1861 cggcccccct tcgcggttcg tcctcgcacc gatcaccgag tttgcatacg cgacggccga
     1921 agactcagac caggcgaggt gatcgccaaa ccgcgtcatg tacaggtaat acggcgtgca
     1981 ggtgcactcc atggtcaccc caaggcgcct gtaggcatcc agcacctgct cctgcacccg
     2041 ggcaaattcc ggggtgatcc ccatctcccg ccaccgtgag cgatccatgc ccaccgggtt
     2101 gaggaccgtc gggacagaga cctttgtgtc gagctgctgc agccagtcga ggccaaagcg
     2161 gcctatcgtc ttgtaggagg ccccgctcac ctgggcgctc cggatgggga tcagccgctc
     2221 cgcgccgtag actttcccca gcgccaagag gatctcgagc atcctggccc tcgtctcgcc
     2281 ctcttccccg gcgaggatgg cctcctcttc agccgagagg atcattgccc ttcctgccag
     2341 ccgacacgca ggaactcggc ctcctggccg aggaccatcg tcgcgtccac tcccaccttc
     2401 acgttggtcc cgtcccccac cctgcagggg tcaagggacg agccccttgc gccggtgacc
     2461 accaagatgt cccggtcacc cctgaccctt gtggcaaggg caaactcgac ttccgcgggg
     2521 tcggaaggat cgatgtcctc gtcgaccacg attacgtgct tcagggaggt gtgggcggcg
     2581 aacgcggcca tgatggcatt tttggcgtcc ccctgg
//
LOCUS       DAOQVJ010000211         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_47468_length_2609_cov_4.014487, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000211 DAOQVJ010000000
VERSION     DAOQVJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47468_length_2609_cov_4.014487"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKL26_05260"
     CDS             <1..310
                     /locus_tag="PKL26_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNS83138.1"
                     /translation="VILSLFSTIGFVRMARTERFGEAFNFGAILETIRKIGWGSYILA
                     LIILFIVAGIIWFVINLLSAIPFIGWLIALVLLPFMIIFEARYITRVYEASGAVPAAS
                     "
     gene            383..1891
                     /locus_tag="PKL26_05265"
     CDS             383..1891
                     /locus_tag="PKL26_05265"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNS83139.1"
                     /translation="MVLLSGNIRFFDTTLRDGEQTPGVSLNPGQKLEIATMISDIGVH
                     VVEVGSVAASDGEREALRLISDAGLNAEVCTYVRAVSQDIDSAADYGADSVHLVIPVS
                     DLHIEKKMRKTRAQICEMAFSAVTYARERGLIVELSGEDASRADPAFLREIYAGGLER
                     GAERLCFCDTVGLLTPERVAEVIPQLCLAPLSIHCHDDLGMAMANTVAALRAGAGCAH
                     VTVNGIGERAGNTPLEELVMTLELLYRHPTGIRTEEIYRLSSLVSRMTGVPLPTNKAI
                     VGEMAFTHESGIHAHGVLREPSTYEPVPPERVGRKRRIMLGKHSGSASVEAALAEMHY
                     HPDESQLKEILKRIKVLGDKGSRITDTDLIAIAEAVMAIECRPKLKMKQFTVVSGSNV
                     IPTASVTMDVRGEEVTGASTGAGPVDAAMEALRRSVAAVADIRLEEYHVDAIHGGTDA
                     LVDVTVRLSKDGKIITSRGARTDIIMASVEAVIAGMNRLLREENEDRSQNTH"
     gene            1863..>2609
                     /locus_tag="PKL26_05270"
     CDS             1863..>2609
                     /locus_tag="PKL26_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNS83140.1"
                     /translation="MKTGAKILIEGLQREGVETIFGYPGGVVLPIYDELYHSSLRHIL
                     VRHEQAAAHAADGFARASGRVGVCLATSGPGACNLVTGIATAYMDSVPIVALTGQVPT
                     SLLGNDAFQESDITGITLPITKHNYLVKRTEDLGHVVREAFYISRTGRPGPVLIDLPK
                     DVSTKEIEYVYPESEQVNLRGYQPTYRGHTRQIDKAIEMMMEAERPLFYVGGGVISSN
                     ASGDLIQLAEMMGIPVTTTLMGIGAIPCDHP"
BASE COUNT          546 a          749 c          819 g          495 t
ORIGIN      
        1 cgtcatcctc tcgctcttct ccaccatcgg gtttgtccgg atggcgagga ccgaaagatt
       61 cggcgaggca ttcaatttcg gggcgatcct cgagacaatc aggaagatcg gctggggcag
      121 ttacatcctt gcgttgatca tcctctttat cgtggcggga atcatctggt tcgtgatcaa
      181 cctattgagc gcgattccct tcatcggctg gctcatcgcg ctggtcctcc ttcccttcat
      241 gatcatcttc gaggcccgct acatcaccag ggtctacgag gcctcgggtg cggtaccggc
      301 tgccagctga ggtcgcattc aacccacttt ttttataccc gtgggcacaa aggtacagtt
      361 cgtggtggaa tttccatgac agatcgtttt attgtccggt aacatccggt ttttcgacac
      421 cacccttcgg gatggggagc agacccccgg tgtttcgctg aatcccggtc agaagcttga
      481 gatcgccacg atgatctcgg atatcggggt gcatgtggtg gaagtcggct ccgttgcggc
      541 atccgatgga gaacgcgaag ccctccggct tatctccgat gcaggcctga acgcggaagt
      601 gtgtacatat gtcagggcag tatcccagga catcgattct gcagccgatt atggcgctga
      661 ttcggtccac ctcgtcatcc cggtgagcga cctgcacatc gagaagaaga tgaggaagac
      721 cagggcgcag atatgcgaga tggccttctc tgcggtcact tacgcacggg agcgcgggct
      781 catcgtcgaa ctctcagggg aggacgcgtc gagggcagac ccggcattcc tccgggagat
      841 ctatgccggc ggccttgaac gcggagcaga acgcctctgt ttctgcgaca cggtaggact
      901 gctgacgccc gaacgcgtgg cagaggtgat cccgcagctc tgccttgcac ctttgagcat
      961 ccactgccat gacgacctgg ggatggcaat ggccaacacg gtggcagcgc tccgggcagg
     1021 cgcagggtgt gcacacgtca cggtcaacgg catcggcgag cgggccggga atacgcctct
     1081 cgaagaactc gtgatgaccc tcgaactgct ttaccggcat ccgaccggga tccggaccga
     1141 ggagatctac cggctctcct ctctcgtgtc ccggatgaca ggggtccctc ttccgacaaa
     1201 caaggccatc gtgggggaga tggcgttcac ccacgagagc ggaatccatg cacacggcgt
     1261 cctgcgggag ccgagcactt acgagcccgt gccgcccgag agggtaggga ggaagcggcg
     1321 catcatgctc gggaagcact cgggatcggc ctctgtcgag gcggcgctcg cagagatgca
     1381 ctatcaccct gacgagagcc agttgaaaga gatcctgaag cggataaaag tgttggggga
     1441 caaggggtca aggatcaccg ataccgacct tattgccatc gcagaagcag tgatggcgat
     1501 tgagtgccgg ccgaaactga agatgaagca gttcacggtc gtaagcggga gcaacgtcat
     1561 ccccaccgca tcggtcacga tggatgtgag aggcgaggag gtgacgggcg cctccacggg
     1621 tgcggggccc gtggatgctg ccatggaggc actccggagg tcggtcgccg ccgtcgccga
     1681 catacggctc gaggagtacc acgtcgatgc gatccatggg ggaactgatg cgctcgtgga
     1741 tgtgacggta agattaagta aggacggaaa gataattacg tctcgcggcg cccgcacgga
     1801 catcatcatg gcaagcgtcg aagcggtgat cgccggcatg aacagacttc tgagggaaga
     1861 gaatgaagac cggagccaaa atactcattg aagggctgca acgcgaaggg gtagagacaa
     1921 tattcggcta ccccggcggt gtagtgttgc cgatctatga tgaactgtac cattcgtcat
     1981 tgcggcacat ccttgtacgg catgaacagg cagccgccca tgccgctgac gggttcgccc
     2041 gtgccagcgg cagggtgggc gtatgcctgg ccacctccgg tccgggggcc tgcaacctgg
     2101 tgaccgggat tgcaaccgcc tacatggact ccgtccccat cgttgcgctg acggggcagg
     2161 tcccgacaag cctgctcgga aacgacgcgt tccaggagtc ggacatcacc ggtatcacgc
     2221 tgcccatcac caagcacaac tacctcgtga agcggaccga ggacctgggg cacgtcgtca
     2281 gggaagcatt ctacatctcc cggacgggaa ggccagggcc cgtgctgatc gatctcccca
     2341 aggatgtcag cacgaaggag atcgagtacg tatatcccga gtcggagcag gtgaatctcc
     2401 ggggatacca gccgacctac agggggcata cccgccagat agacaaggcg atcgagatga
     2461 tgatggaggc ggagcgcccc ctcttctatg tcggtggagg ggttatctcg tcgaacgcat
     2521 ccggagatct catccagctc gccgagatga tgggtatccc tgtgactacc accctcatgg
     2581 ggatcggggc catcccgtgc gaccatccc
//
LOCUS       DAOQVJ010000212         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_47905_length_2594_cov_4.023237, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000212 DAOQVJ010000000
VERSION     DAOQVJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47905_length_2594_cov_4.023237"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="surE"
                     /locus_tag="PKL26_05275"
     CDS             complement(<1..594)
                     /gene="surE"
                     /locus_tag="PKL26_05275"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNS83141.1"
                     /translation="MKPRVLLTNDDGINSAGLWAAYEALSPIAQVTVVAPATQQSAVG
                     RSISIFEPIRAHEVEMNGVRAWAVGGKPTDSVIIGLYALDLQPAMVVSGINIGENLSY
                     ESIMTSGTVGAALEAANQGTPAIAFSLQVEDQGDKFDDPRHHAQSFEESKRVVKEVCG
                     MFLDRRLPPGADILNVNIPSVVKGGYEVTRLGEKLFST"
     gene            676..1563
                     /locus_tag="PKL26_05280"
     CDS             676..1563
                     /locus_tag="PKL26_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNS83142.1"
                     /translation="MNAFLAEYTVFHDPHLAPEGEAMLRVLSESFARCGYEVLTPEDG
                     DFLGEIRRLAPLCEVGLVIAPDHLLFQFSSLLERLTHSVGCGSMNAAVCADKVRCGQI
                     LARNGVPVPGSGSTGTQVVKPALGCGSVGVRLTKEPPGKGEYAQDYIEGEHLSVSLVG
                     SRVVGDACSFYTGKPPLLLAVNRQEIVVGDDGLFHYLGGETPLLHPRQEELVETATKA
                     VQVLGCQGYVGVDIVLADRCYVVDVNPRVTTSIVGIAACMEEEIADILVQASRGGGPD
                     EVHLNGKARFDSQGRVKRL"
     gene            1560..2492
                     /locus_tag="PKL26_05285"
     CDS             1560..2492
                     /locus_tag="PKL26_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNS83143.1"
                     /translation="MIGIDIGGANVKIATDDGVETIYCPLWEGAPLGEILSRYRTADG
                     AAAVVMSGELADCFCTKMEGIAWITKTVREVFPAALFYGTDGRFYDSAVPALAAANWL
                     ASAACLHARDPDGLLVDMGSTTTDIVPLRPLSALYGLTDLLRLQKGYLVYTGLLRTTV
                     PAVIRTVTLEGVPTPISAEQFAISADAHLVLGHIGEADYSCDTPDRKGRDRIACLRRL
                     ARVVCADLDEIGEEGAVQVAETFWEAQQDTIRGAVRKAMEESGAIRIYTAGAGSGLLA
                     ASLGGVDLSVELGAGASALPAYAVSEVLRRSRGC"
BASE COUNT          451 a          817 c          846 g          480 t
ORIGIN      
        1 ggtcgagaag agcttctccc caaggcgtgt cacctcgtaa ccgcccttca cgacagaagg
       61 gatgttcacg ttgaggatgt ctgccccggg tggcagccgc cggtccagga acatgccgca
      121 cacctctttc acgacgcgtt ttgattcctc gaaactctgc gcatggtggc gcgggtcatc
      181 gaacttgtcc ccctggtcct ctacctggag agagaaggcg atcgcagggg tcccctggtt
      241 cgccgcctcg agcgccgctc ccacggtccc tgatgtcatg atggactcat acgagaggtt
      301 ctcgccgatg ttgatgccgc tcaccaccat tgccggctga aggtcgagcg catagagccc
      361 gatgatcacc gagtccgtgg gctttcctcc cactgcccat gctcgcacgc cgttcatctc
      421 cacctcgtgc gccctgatcg gctcgaagat ggagatggat ctccccaccg cgctctgctg
      481 ggtcgcgggg gccaccaccg tcacctgggc gatgggagag agggcttcgt atgcggccca
      541 gaggccggcg gagttgatgc cgtcgtcatt cgtgagcagg acacggggtt tcatggtcac
      601 caacccgttg ggggagcgat gagaaatact tgaccgatcg tcatcctctt ctggcacggc
      661 gccccattca atacgatgaa cgcgttcctg gcagagtaca ccgtcttcca cgacccacat
      721 ctcgcccccg aaggcgaggc aatgctccgg gtcctgtcag agagtttcgc acggtgcggg
      781 tacgaggtgc tcacccctga ggatggcgac ttcctcggag agataaggag acttgccccc
      841 ctctgcgaag ttggcctcgt gatagccccc gatcacctcc tcttccagtt ctcctctctc
      901 ctcgagaggc tgacacacag cgtggggtgc gggagcatga acgccgccgt ctgcgcagac
      961 aaggtacggt gcggccagat cctggccagg aacggagtcc cggtgccggg atcgggatcg
     1021 acgggaacgc aggtggtcaa gcccgcgctc ggctgcggct cggtgggggt ccgcctgaca
     1081 aaagagcctc ccggcaaggg ggagtatgcg caggactaca tcgagggcga gcacctgagc
     1141 gtgagcctgg tggggagcag ggtggtcggc gacgcgtgct ccttctacac cggcaagcca
     1201 cccctcctcc tcgcggtgaa ccgccaggag atcgtggtgg gggacgacgg gctgttccat
     1261 tacctcggcg gggagacgcc actcctccac cccaggcagg aggaactcgt agagaccgcg
     1321 acgaaagccg tgcaggtcct cggctgccag gggtacgtgg gcgtcgatat cgtgcttgcc
     1381 gaccgctgtt acgtcgtcga cgtgaacccg cgggtcacca ccagtatcgt ggggatcgca
     1441 gcctgcatgg aagaggagat cgcagacatc ctcgtccagg catcgagagg cgggggtccc
     1501 gatgaggtgc acctgaacgg gaaggccagg ttcgacagcc aggggagggt gaaacggctg
     1561 tgatcgggat cgatattggc ggggccaatg tcaagattgc aacagacgat ggggtggaga
     1621 ccatttactg ccccctgtgg gagggggctc cgcttgggga gatcctctcc aggtaccgca
     1681 ctgcggacgg cgctgcggcg gtggtgatga gcggggagct cgcggactgt ttctgcacca
     1741 agatggaagg gattgcctgg atcacaaaaa ccgtgcggga ggtctttccg gcggcgctct
     1801 tctacggcac cgacggtcgc ttctacgact ccgccgtgcc ggcgcttgcg gcagcgaact
     1861 ggctcgcatc cgccgcatgc ctccacgccc gcgaccctga cggcctcctg gtggacatgg
     1921 ggagtaccac gaccgacatc gtcccgctgc gcccgctctc cgcgctctac ggacttaccg
     1981 atctcctgcg gctccagaag ggctacctgg tctacaccgg gctcctgcgc accactgtcc
     2041 cagcggtcat ccggacggtc acgctggaag gggttccgac cccgataagc gccgaacagt
     2101 ttgccatcag cgcagacgca cacctggtgc ttggacacat cggggaggcg gactactcct
     2161 gcgacacccc ggacaggaag ggacgggacc gcattgcctg tctccggagg cttgcacggg
     2221 tggtctgtgc cgaccttgat gagatcggag aagagggcgc agtccaagtt gcagaaacct
     2281 tctgggaggc gcagcaggat acgatcaggg gagcagtaag gaaggcaatg gaagagtccg
     2341 gggccatccg catctacacc gccggggctg ggtcggggct ccttgccgcg tcgcttggtg
     2401 gcgtggacct ctccgtggaa cttggggccg gtgcctctgc cctccccgca tacgccgttt
     2461 cagaggtgct tcgacgaagc cgtgggtgct gagcctcatc ctcctggcag gatcgttgct
     2521 ggcgagtgcc gcgctctggc ttgcaggcat gccgttcttc ttcctcttcc tgttcatccc
     2581 tctcattccc ttcc
//
LOCUS       DAOQVJ010000213         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_48224_length_2582_cov_4.705580, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000213 DAOQVJ010000000
VERSION     DAOQVJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48224_length_2582_cov_4.705580"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKL26_05290"
     CDS             <1..182
                     /locus_tag="PKL26_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNS83144.1"
                     /translation="SSAGLRVLLAAMKRLKKAEGNLVLCAMKPFVQEVFDLTGFSRIF
                     TICDSEEDATHHLGP"
     gene            289..1653
                     /locus_tag="PKL26_05295"
     CDS             289..1653
                     /locus_tag="PKL26_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNS83145.1"
                     /translation="MHTKIVLDEDHMPKKWYNILPDLPTPLDPPLHPATGKPLVPEDL
                     TPIFPMELILQEMSQKRTIDIPSDVQDVLRLWRPSPLYRAHRLEKYLKTPAHIYYKYE
                     GVSPPGSHKPNTAVPQAYYNMKAGTERIATETGAGQWGSALAFATQIFGLECTIYMVR
                     SSYMQKPYRKSMMQVWGAECIPSPSTKTKAGTAVLQKDPETTGSLGIAISEAVEDAAT
                     HGNTNYSLGSVLNHVCLHQTVVGLECMEQLASVEDYPDVVIGCVGGGSNFAGISFPFA
                     GEKLKGKHKETEIIAVEPASCPTLTKGLFAYDYGDVAGLTPLLKMFTLGHDFVPPSIH
                     AGGLRYHGDNPTISRLVYDGVMKAVSYHQSEVFDAAQTFARTEGIIVAPETSHAVRAT
                     IDEALKCKKSGEAKTLLFNCSGHGNFDMSAYDAFYANQLVDYEYPEQLIRESLARLPK
                     IPGA"
     gene            1747..>2582
                     /locus_tag="PKL26_05300"
     CDS             1747..>2582
                     /locus_tag="PKL26_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNS83146.1"
                     /translation="MCTIGFLVNPFAGMGGAVGLKGTDGMVEAALARGAVPRAPGRAA
                     ETILQVADIGAHFITCSGVMGEDILAGAGIPGYEVVYRPASETTGAADTMAACRAFLD
                     RDADLILFCGGDGTARDVYSVIGDLVPMLGIPAGVKMYSAVFAIHPRAAAGIVREGCT
                     PGGKFLLRDAEVVDVDEEAYREGSLRTRLFGIAKSPYRPGLVQGTKQVFEDSDEERAK
                     KDIARFMREVIEGTPGILYILGPGSTTAAIGEEIGLKKTLLGFDAVKGGRLIATDLNE
                     EG"
BASE COUNT          600 a          725 c          750 g          507 t
ORIGIN      
        1 tgagcagcgc aggcctgaga gtcctcctcg cggcaatgaa acggctgaaa aaggcagaag
       61 ggaacctggt cctctgtgcc atgaaaccat ttgtccagga ggtttttgac ctgaccgggt
      121 tctccaggat atttaccatc tgcgattcag aggaagacgc aacccaccac ctcggcccct
      181 gaataatcca ttttcaagat attcactatt ttagaacacc aggatacctg gcatcaataa
      241 tcatagttgt acaacaatgt atatatttat acacggtaaa tgatgggtat gcataccaag
      301 atcgttcttg atgaggacca catgcccaaa aagtggtaca acatactgcc agacctccct
      361 accccgctgg atcctccgct gcacccggcc acgggaaaac cgcttgtccc ggaagacctg
      421 acccccatct tcccgatgga acttatcctc caggagatga gccagaaacg caccatcgat
      481 attccttccg atgtgcagga cgtgttgagg ctctggaggc cgagcccgct ctatcgcgct
      541 caccgtctgg agaagtatct gaagactccc gcccacatat actacaagta cgagggagtg
      601 agcccacccg ggagccacaa gcccaacact gcggttccgc aggcctatta caacatgaaa
      661 gccgggaccg agcgcattgc aactgagacc ggcgcaggcc agtggggctc ggccctcgcg
      721 ttcgcaaccc agatcttcgg gcttgagtgc accatctaca tggtcaggtc tagttacatg
      781 cagaaaccct accgcaagtc gatgatgcag gtatggggag cagagtgcat cccgagcccg
      841 agcacgaaga cgaaggccgg aaccgccgta cttcagaagg accctgagac cacggggagc
      901 cttggaatcg ccatatcgga ggcagtagag gatgcggcca ctcacgggaa caccaattac
      961 tcccttggct ccgtcctgaa ccacgtctgc ctccaccaga cggtggtcgg actggaatgc
     1021 atggagcagc tcgcatccgt cgaggattat ccggacgtgg taatcggctg cgtgggcgga
     1081 gggtccaact tcgccgggat ctcgttcccg tttgccggtg agaagctgaa aggaaagcac
     1141 aaggagaccg agatcatcgc ggttgaaccg gcatcctgcc cgacgctcac gaaggggctc
     1201 ttcgcctatg actatggtga cgtggccgga ctgactccgc tcttgaagat gttcaccctc
     1261 ggccatgact tcgtgccgcc ctccatccat gcagggggac tacgctacca cggcgacaat
     1321 ccgacaatct cgaggctcgt gtatgacggg gtgatgaaag cggtctcgta ccatcagagc
     1381 gaggtctttg atgccgcgca gacgtttgcc aggacagagg ggatcatcgt cgctcccgag
     1441 acttcgcatg ctgtcagggc gactatcgat gaagcactca aatgcaaaaa gagcggagag
     1501 gcaaagaccc tcctctttaa ctgttcaggg cacggcaact ttgacatgtc tgcgtatgat
     1561 gcgttctatg ccaaccagct cgtggattac gagtatcccg agcagctgat acgcgagtcc
     1621 cttgcccgtc tcccgaagat cccgggcgca tgacaggaga acgggttacc acaaaagact
     1681 cccccatttt ttgccattag taccccaaca catatcaccc cctcctccat cagttatttc
     1741 acgcgcatgt gcaccatagg gttcctggtc aatccctttg ccggtatggg cggagcagtg
     1801 ggcctgaaag ggacggatgg aatggttgag gcggcccttg ccaggggtgc cgtaccccgg
     1861 gctccgggga gggcggcaga gacgatattg caggtcgcgg acatcggggc tcatttcatt
     1921 acctgttccg gggtcatggg ggaggatatc ctggcaggag caggaatccc gggatacgaa
     1981 gtagtatatc gtcccgcttc agaaacgacg ggagcagcgg ataccatggc agcatgcagg
     2041 gcgtttcttg acagggatgc agacctcatc ctcttctgcg gcggtgacgg cactgccagg
     2101 gatgtatatt ccgtgatagg ggacttggtc cccatgctcg gaatccccgc aggggtgaag
     2161 atgtactcgg cggtctttgc cattcaccct cgcgcagccg cagggatagt gcgcgaggga
     2221 tgcacaccgg gaggcaagtt tctcctccgt gatgcggagg tggtggacgt ggacgaggaa
     2281 gcatacaggg aaggatcgct acggacccgc ctctttggta tcgcaaagag cccgtaccgc
     2341 ccaggcctcg tgcaggggac aaagcaggtg ttcgaggatt ctgatgagga acgtgcaaag
     2401 aaagatattg cacggtttat gcgggaggtg atcgaaggga cccccggtat tctctacatc
     2461 cttggcccgg ggagcaccac tgcggccatc ggggaggaga tcggcctgaa aaagaccctc
     2521 ctcggcttcg atgccgtgaa aggaggaagg ctcatcgcca cggatttaaa cgaggaggga
     2581 tt
//
LOCUS       DAOQVJ010000214         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_48254_length_2581_cov_3.650040, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000214 DAOQVJ010000000
VERSION     DAOQVJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48254_length_2581_cov_3.650040"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1597)
                     /locus_tag="PKL26_05305"
     CDS             complement(122..1597)
                     /locus_tag="PKL26_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNS83147.1"
                     /translation="MKFFEKVLIANRGEIAIRVMRACRELDIETVAIYSTADSDALHV
                     KYADEAFLLGEAPPAKSYLNMERILDITKKSGAEAIHPGYGFLAENYRFSKLCQEEGV
                     VFIGPSWKTIRAMGSKIESKQMMREADVPVLPGTLEGVQDPEDAKKVADEVGYPVIVK
                     ASAGGGGIGMHIVQDEKGLEEAIDAGRRIAQSAFGDATVFIEKYLERPRHIEFQVLAD
                     EHGNVIHLYDRECSIQRRHQKLVEEAPCPIMTPDLRERMAASAIAVARASDYTNAGTV
                     EFLYADGNYYFMEMNTRLQVEHTVTEMITGRDIVKHQIAIAAGESLPLDQEDVTIRGH
                     AIECRINAEDPLNNFAADPGKILRYRSPGGPGMRVDSGIHMGYSIPPTYDSMISKLCA
                     WGADRSEAIARMRRAIYEYVIIGIKTTLPLHHAIMHNRHYINGDTHTHFLQEEHMQKT
                     LARYVREEETRMQTLAASLRQGKQIAAITGAVNVYLNSQKK"
     gene            complement(1594..>2581)
                     /locus_tag="PKL26_05310"
     CDS             complement(1594..>2581)
                     /locus_tag="PKL26_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNS83148.1"
                     /translation="AALSGSPGDTGIDLRVLKDVRNACVKVREKYEGLVDPISERVDS
                     DVLLYQLPGGMISNLVSQLKEQDALDKVNAMFEEVPRVRKDLGYPPLVTPTSQIVGTQ
                     AVFNVLIGGERYRNVTNEVKDYVRGLYGKPPGPINDEIRRLIIGDEPPITVRPADLLE
                     PIYEKMKAQATSEGLIKKEEDVLTYILYPAIAPAFLRGEKKPEEIPQKKAKVSDVPSI
                     PPSMEVEVDGEVFSVRIVSVGGSAVVSAAPVQEKIPRGEIQGGVKSNMQGMVLKILVN
                     RGAEVKKGDTLLVLEAMKMENPIHSPADGRVADIFVDTGDTVKSGDVLLVVE"
BASE COUNT          450 a          814 c          734 g          583 t
ORIGIN      
        1 tatgagtctg gcgccctaac cagctaggcc accgcagcgc acaaaagtgc ataattaaaa
       61 cgcagtatac gctcttaaag gttctgtttt gtgagaaaac ggtgcgtggg ggtagttctc
      121 ctcacttctt ctggctgttg aggtagacgt tcacggcgcc ggtgatggcc gcgatctgtt
      181 ttccctggcg aagtgatgcc gcgagcgtct gcatccgggt ctcctcctca cgcacgtacc
      241 gggcaagggt cttttgcatg tgctcctcct gcaaaaagtg ggtatgggta tccccattga
      301 tgtaatgccg gttgtgcatg atggcatggt gcaggggaag cgtcgtcttg atgccgatga
      361 tgacgtactc gtagatggcc ctgcgcatcc gggcgatggc ttcgctccgg tcggcacccc
      421 aggcacagag cttcgagatc atcgagtcgt acgtgggggg gatggaatac cccatgtgga
      481 tcccggagtc tacccgcatc cccgggcctc ctggtgagcg gtaccggagg atctttcccg
      541 gatcggcggc gaaattgttc agcgggtcct ctgcattgat ccggcactcg atcgcatggc
      601 cccggattgt cacgtcctcc tggtcaaggg ggagggattc tccggcagcg attgcgatct
      661 ggtgcttgac gatatccctg ccggtgatca tctcggtcac cgtgtgctcg acctggagcc
      721 gcgtgttcat ctccatgaag tagtaattcc cgtctgcata gaggaactca accgtgccgg
      781 cattcgtgta gtccgacgcc cgtgccaccg cgattgcgga tgctgccatc ctctcccgca
      841 gatctggggt catgatcggg cagggtgcct cctcgacgag cttctggtgc ctccgctgga
      901 tcgagcactc gcggtcatag aggtggatga cgtttccgtg ctcgtctgcg agtacctgga
      961 actctatgtg cctgggcctc tccaggtact tctcgatgaa gacagtcgcg tcaccgaatg
     1021 cagactgggc gatgcggcgg ccagcatcga tcgcctcctc gaggcccttc tcgtcctgca
     1081 cgatgtgcat gccgatgcca cctccccccg cgctcgcctt tacgatgacc gggtagccca
     1141 cctcatctgc aaccttcttt gcatcctccg ggtcctgcac gccctcaagt gtaccgggaa
     1201 ggacagggac atcggcctcc ctcatcatct gcttgctctc gatcttcgaa cccatggccc
     1261 gaatcgtctt ccaggagggg ccgatgaaga ccactccctc ttcctggcag agtttcgaaa
     1321 accggtagtt ctctgcgagg aacccgtacc cggggtgaat cgcctcggcc cccgacttct
     1381 ttgtaatgtc gaggatccgc tccatgttga ggtagctctt cgcagggggt gcctctccca
     1441 ggaggaatgc ctcgtccgcg tacttgacgt gaagcgcatc tgagtcagcg gtggagtaga
     1501 tggccacggt ctcgatatca agctcccggc aggccctcat cacccggatg gcgatctcgc
     1561 cgcggttggc gatgaggacc ttctcgaaga atttcattcc accaccagga gcacgtcacc
     1621 gctcttgacg gtgtccccgg tatccacaaa aatatccgca acacggccgt cagccgggct
     1681 gtggatgggg ttctccatct tcatggcctc gagcacgagc agcgtgtccc ctttcttcac
     1741 ctctgccccg cggttcacaa ggatcttgag gaccatcccc tgcatgttcg acttgacacc
     1801 gccctgaatc tcccccctcg ggatcttctc ctggaccggt gcggcactga cgactgcact
     1861 tcccccgacc gagacgatcc tgacggaaaa gacctccccg tccacctcga cctccatcga
     1921 ggggggaatc gagggcacgt ccgagacctt ggccttcttt tgggggatct cctccggctt
     1981 cttctctccc cggaggaacg cgggggcaat cgccgggtag aggatatagg tgaggacatc
     2041 ctcctccttc ttgatcaatc cttcagaagt tgcctgggcc ttcatcttct catagatcgg
     2101 ctccaggagg tccgccggcc ggaccgtgat gggcggctcg tcaccgatga tcagcctccg
     2161 gatctcgtcg ttgatggggc ccggaggttt tccgtagagc cccctcacgt agtccttcac
     2221 ctcgtttgtg acattccggt accgctcacc gcctatcagt acgttgaaga ccgcctgcgt
     2281 cccgacgatc tgggaggtgg gagtgacaag gggcgggtag ccaaggtcct tcctcacccg
     2341 cgggacctcc tcgaacattg cgttcacctt gtcgagagcg tcctgctctt tcaactgcga
     2401 gacgaggttt gagatcatcc ccccggggag ctggtagagg aggacatcgc tgtccacccg
     2461 ctccgatatg gggtcgacaa gcccctcata cttctcgcgg accttcacac aggcattcct
     2521 gacatctttc aggacacgga gatcgatccc cgtgtccccc ggacttcctg aaagtgctgc
     2581 c
//
LOCUS       DAOQVJ010000215         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_48860_length_2561_cov_4.558659, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000215 DAOQVJ010000000
VERSION     DAOQVJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48860_length_2561_cov_4.558659"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKL26_05315"
     CDS             <1..211
                     /locus_tag="PKL26_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00297 family protein"
                     /protein_id="HNS83149.1"
                     /translation="VISLLAFLLGIVDLPTAAACMLAGFIGTNIDSVVGALMENPGLV
                     GNAGTNFLATLGGGVCAIALSYPWW"
     gene            405..986
                     /locus_tag="PKL26_05320"
     CDS             405..986
                     /locus_tag="PKL26_05320"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HNS83150.1"
                     /translation="MERSEEYFCPVCDEETGHEVISRGRNPIARCLSCGSVHPYSPEK
                     EKKLVQVKAIVSAEGTSRVCRVEMEEGEPCRVGDHFVAECGDEYIGVEVMAIERGESR
                     VRKAISSEIATLWTRKVEEVAVRISLHSGRTTASLRTLVPGEEPFVIGEVYKVGQKRI
                     RISRIKMRRGMMLRREGAKAIAREIRRIFAYPL"
     gene            complement(981..1247)
                     /locus_tag="PKL26_05325"
     CDS             complement(981..1247)
                     /locus_tag="PKL26_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNS83151.1"
                     /translation="MAIRILYFSQDGCMACSEQEPILLEVEKNCGVKVERIAIDPTKG
                     RSLIKEHRLSVTPTILFVREGKELERFEGLVHQEQLSEAIRRHL"
     gene            complement(1292..1996)
                     /locus_tag="PKL26_05330"
     CDS             complement(1292..1996)
                     /locus_tag="PKL26_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83152.1"
                     /translation="MKAQEKGKGKKKPASRWTAKKIGIIIIGVGFAAIMVVSSLGMGW
                     LVSMNPAAAGDGAVVDVTFFDGMDRPVLTTNPRTFNATFEAGNMVWLCGQFSTRVKST
                     SQTELISIPIYNYYYGEAKYALFSPEWDTISSELEGMKQGESRRITFPALEGFEREMT
                     AEEFNGIGGNFTTVTNGDQVVLAFAENPEISLEENVTPSYVIRIGYVTAKTDEGIRVN
                     YACPSAQISLVQLTKA"
     gene            2218..>2561
                     /locus_tag="PKL26_05335"
     CDS             2218..>2561
                     /locus_tag="PKL26_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HNS83153.1"
                     /translation="MVYRRRYPFGWMMRDFEDLMGEMENRFWGGGGRLLPPGGVADRM
                     LPAIRGEFRVDVREHEDEVMIVADLPGVDKEDVSISLLNPNTLEISCERKMDKEEKEE
                     GYYVRERMYGSMA"
BASE COUNT          570 a          614 c          787 g          590 t
ORIGIN      
        1 tgtcatctca ctgcttgcgt ttctccttgg catcgtcgat ctcccgaccg ctgcggcctg
       61 catgcttgca gggttcatcg ggaccaatat cgacagcgtg gttggggcat tgatggagaa
      121 cccgggtctt gttggaaatg ccggtacaaa cttccttgcg acgcttggcg gaggggtctg
      181 tgcaatagcc ctctcatatc cgtggtggta agggaggaac ctctttctga aaggccccct
      241 ggttgcaaaa acccccacta tctatcatgc cagggcagtc atttcaccgg gatgtgcggg
      301 agagagggtg gcgccaggtg ccattgtttc gaatttgagg tagaggggcc agagtgtata
      361 tttcatgaaa gatggaatgg tataccagga atggtgatgg ggaaatggag aggagcgaag
      421 aatacttctg ccctgtgtgc gatgaagaga ccggtcacga ggtgatctcg cggggaagga
      481 acccgattgc caggtgcctc tcgtgcggga gcgttcaccc ctattcaccg gaaaaagaga
      541 agaagcttgt ccaggtgaag gccatcgtca gcgccgaagg gacatcgagg gtctgcaggg
      601 tcgagatgga ggagggcgag ccctgccgtg tgggagacca ttttgtggca gagtgcggtg
      661 acgagtatat cggtgttgag gtgatggcaa tcgagcgcgg tgaatcgagg gtaagaaaag
      721 cgatcagcag cgagattgcc acgctctgga cgagaaaggt tgaggaagtg gcggtgagga
      781 tctctcttca ttcgggccgg accaccgcgt ctcttcggac tctggtgcca ggggaagagc
      841 cgttcgtgat aggggaagtc tacaaggttg gccagaagag gatcaggatc tccaggatca
      901 agatgcggag ggggatgatg ctccgcaggg agggtgcaaa ggccattgcc cgcgagatcc
      961 ggaggatttt tgcgtatccg ctataggtgc cggcggattg cctcgctgag ctgctcctgg
     1021 tgaaccagcc cctcgaaacg ttccagttct tttccctcac ggacaaagag tatggttggg
     1081 gtgacactca ggcggtgctc tttgatgagt gagcgtccct tcgtagggtc gattgcgatc
     1141 ctctccacct tcaccccgca gttcttctcc acctcaagaa gtatcggttc ctgttcgctg
     1201 caggccatgc acccatcctg ggagaaatag agtatccgga ttgccatgca gaaaaaaacg
     1261 aggggggatt ataaaaaagc tatggaattt ttcaggcttt ggtgagttgt acaagggaga
     1321 tctgggccga tgggcaggca tagttgaccc ggatgccttc gtcggtcttt gcagtgacgt
     1381 atccgatgcg gatcacataa ctgggggtga cattctcttc aagcgagatc tcggggttct
     1441 cggcaaaagc aaggactacc tggtctccgt ttgtgacggt ggtaaagtta cccccgatcc
     1501 cattgaactc ctctgcagtc atttcgcgct cgaatccctc gagcgcgggg aatgtgatcc
     1561 tgcgggattc accctgtttc attccttcca gttcgcttga gatagtgtcc cattcaggag
     1621 agaagagggc atatttcgcc tcgccgtaat agtagttgta tatggggatg gagatgagct
     1681 ccgtctggct ggtgcttttc acccgcgtcg agaactggcc gcagagccat accatgttcc
     1741 ctgcttcgaa tgtcgcattg aaagtcctcg gattggttgt cagcacgggg cggtccatcc
     1801 cgtcaaaaaa ggtgacatcc accaccgccc cgtcgcccgc agcagcaggg ttcatggaca
     1861 caagccaccc catcccgaga gagctgacta ccatgattgc cgcaaaaccg actccgatga
     1921 taataatgcc gatcttcttg gcagtccagc gtgaagcggg tttcttcttc ccctttcctt
     1981 tctcttgagc cttcatcctt ttatcattgg ttcctgggaa aataaacgtt acagcgtgta
     2041 tgtggagaga gcctctacta ccatgcaaac ctatcgggtc ctttgaaaaa cgatccaggt
     2101 ctgacatggt ctgtggataa ctgggtattt cctcatacaa atggtttata tattataaac
     2161 agtcacataa agttagtcca ttggcctttg ccggtggaga aggaaaggag gtttcagatg
     2221 gtatacagaa ggagatatcc gtttggctgg atgatgcgcg acttcgagga cctgatggga
     2281 gagatggaga accgcttctg gggtggaggg gggcgcctcc tcccccctgg cggagtggca
     2341 gaccggatgc tcccggcaat caggggagag ttccgggtgg atgtcaggga gcatgaggac
     2401 gaggtcatga tcgtagctga tctccccggc gtcgacaagg aggatgtctc gatctccctc
     2461 ctgaatccaa acaccctcga gatatcgtgc gagcggaaga tggacaagga ggagaaggag
     2521 gaggggtact acgtcaggga gaggatgtac ggctccatgg c
//
LOCUS       DAOQVJ010000216         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_49217_length_2549_cov_4.560144, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000216 DAOQVJ010000000
VERSION     DAOQVJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49217_length_2549_cov_4.560144"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKL26_05340"
     CDS             complement(<1..457)
                     /locus_tag="PKL26_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HNS83154.1"
                     /translation="MSRYSLTGNLKEAAFQRGADLFGVADASCFLSPDYQGNRPQEIM
                     PGVTSVVVLGVSVPRGAFSTLPKGRGEYTNTLMAGTATLRVIAFFLARQIEREGYLAT
                     IVPTEGSEFGYWYADRSTLKADLSLKYAAYHAGLGSFGRNHLLINPDFGA"
     gene            complement(470..1702)
                     /locus_tag="PKL26_05345"
     CDS             complement(470..1702)
                     /locus_tag="PKL26_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS83155.1"
                     /translation="MREFDAVQKEIKRLLGQIPDTERKKLAETLERLESRIHLLEHNY
                     NRAIRDRAAVHSLLNKSSNELIERYQTIFEFSGTAMAVIEEDGTISLVNTHFERLFGF
                     SREEVEWKKKFPEFLDESVKEYIVGFHRRRLSGDETVPHYYEAKIITRNGSTREVSFS
                     VGLFPGTGRIITSILDIEERKRAEKAVETAQKKIAILNSITRHDIHNQLTPLYLQIDM
                     ALMHTQDPVLRESLQKIERIGQNIQEQIEFMKLYQDVGVKSPIWQRVADVIRTATAGI
                     PLDGISQEIDVGELEIYADPLLQKVFYNLVENAIRHGGKLTHIRFSVEEDPHGVAIIC
                     VDNGTGVPAKEKEHIFERTFFKHTGFGLFLSREILSITGIGIRETGVPGEGARFEILV
                     PRGMFRVTLPNTLIERAP"
     gene            complement(1699..>2549)
                     /locus_tag="PKL26_05350"
     CDS             complement(1699..>2549)
                     /locus_tag="PKL26_05350"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNS83156.1"
                     /translation="DDSSFELGARIGRWGRERFEEPAFVIAVAGLKNDGEAIVRGILS
                     VSPAGTPLYGGLAGDDGRMEETFVFSSAGCTSDGAVVMVLDRSRVDLSGIVTSGWTGV
                     GMPMTVTSSEGNVLHTLNNRPATKVLQEYLNAGEDDLVETAIMFPLLVQRPDGTVVLR
                     TCLSADFSRGSMIFAGSIPRGAQVRFSSSFGYETIEKSIRDITSFHATRPDADLVIAF
                     SCMARHWAAGTMVGDEIAAALRLWKKPLVGFFTYGEIGQGHTGQCDFHNESLCLVLLT
                     VNPPTP"
BASE COUNT          502 a          750 c          725 g          572 t
ORIGIN      
        1 gggcgccgaa gtcagggttg ataaggaggt ggtttctccc aaacgagcca agccctgcgt
       61 ggtacgcggc gtacttgagc gagaggtcgg ctttcagggt agagcggtcc gcgtaccagt
      121 acccaaactc gctcccctcg gtggggacaa tggtcgcaag gtatccctcc cgttcgatct
      181 gccgtgcgag gaagaaggcg atcacccgga gcgtcgcagt cccggccatc agggtgttcg
      241 tgtactcccc cctccccttc gggagcgtcg agaaggcccc gcgggggacc gagaccccga
      301 gcacgacgac tgaagtgacg cctggcatta tctcctgcgg gcggttcccc tggtagtcgg
      361 gggagagaaa acatgacgcg tccgccaccc cgaagaggtc tgcgccccgc tggaaagccg
      421 cttctttcag gttgccggtc agggagtatc tgctcattgt gccagatcgc tatggggctc
      481 tttctattag ggtgtttggc agggtgaccc tgaacatccc gcgggggacg aggatctcga
      541 accgggcgcc ttctcccggc acccctgtct ccctgatccc gatcccggtg atggagagga
      601 tctcccggct gagaaagagc ccgaaccccg tgtgcttgaa aaaggtcctc tcaaagatgt
      661 gctccttctc tttcgccggc accccggtcc cgttatcgac gcagatgatg gcaacgccgt
      721 gtggatcctc ctcgacagag aacctgatat gagtcagttt ccccccgtgc ctgatggcgt
      781 tttccaccag gttgtagaag actttctgga ggagaggatc tgcatagatc tcaagttcgc
      841 ccacgtcaat ctcctgggat atcccgtcaa ggggaattcc cgcggtagcg gtgcggatga
      901 catctgccac ccgctgccat atgggggatt ttacgccaac atcctggtaa agtttcatga
      961 actctatctg ttcctggatg ttctgcccga tgcgctcgat cttctggaga gattctctca
     1021 ataccgggtc ttgtgtatgc atcagggcca tatcgatctg aaggtaaaga ggggtcagct
     1081 ggttgtggat atcgtgccgt gtgatcgaat tgaggatggc aatcttcttc tgtgcagtct
     1141 ctactgcctt ctcggccctt tttcgctcct caatgtcaag gatggacgtg atgatcctcc
     1201 ctgttcctgg gaagaggccg acgctgaagg agacttccct ggtgctgccg ttccttgtaa
     1261 tgatcttcgc ctcgtagtag tgggggacgg tctcgtctcc tgaaaggcgc ctccggtgga
     1321 acccgacgat atactccttc acgctctcgt caaggaattc cgggaatttc ttcttccact
     1381 ccacttcctc gcgtgagaac ccgaacagtc gttcgaagtg agtgtttacg agcgagatgg
     1441 tcccatcctc ctcgatcact gccatcgcgg ttcccgagaa ctcaaaaatt gtctgatacc
     1501 gctcgatcag ctcgtttgaa ctcttgttga ggagagagtg aacggctgca cgatcacgga
     1561 tcgcccggtt gtagttatgc tccaaaagat ggatgcggct ctccagccgt tccagggttt
     1621 cggcgagctt ctttctctcg gtatcaggta tctgaccaag gagccgctta atctcttttt
     1681 gcaccgcatc gaattctctc atggcgtggg aggattcact gtgagcagca ccaggcagag
     1741 actctcgtta tgaaaatcac actgtccggt gtggccctgg ccgatctcgc cgtaggtgaa
     1801 aaaccccacg agcggcttct tccacaagcg cagtgccgca gcgatctcgt ctcccaccat
     1861 ggtacctgct gcccagtgcc tggccatgca ggagaacgcg attacaaggt ctgcatccgg
     1921 ccgggtggca tgaaacgacg tgatatccct gattgatttc tcaatcgttt cgtatccgaa
     1981 tgaactcgag aaccgtacct gggctcccct ggggattgaa cctgcaaata tcattgaccc
     2041 ccgggaaaag tcggccgaga ggcaggtgcg caggaccacg gtgccgtccg gcctctggac
     2101 caggagggga aacatgatcg cggtttccac gaggtcatct tcaccggcat tcaggtactc
     2161 ctgaaggacc tttgtcgccg ggcggttgtt cagggtgtgg aggacattgc cttcagacga
     2221 tgtgacggtc atcggcatgc cgacccccgt ccaaccgctg gtgacgatgc cggacaggtc
     2281 cacccgggag cggtcgagga ccatgaccac tgcgccatcc gatgtgcagc cagccgaaga
     2341 gaagacgaag gtctcctcca tccttccatc atcccccgca aggcctccat agaggggagt
     2401 gccggcaggg gaaacggaga gtatcccccg gacaatggct tccccgtcgt ttttcagtcc
     2461 cgcaaccgca atgacgaagg ccggttcttc aaatctctca cggccccatc gaccgatcct
     2521 tgccccaagt tcgaaactgg agtcgtcac
//
LOCUS       DAOQVJ010000217         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_50318_length_2514_cov_3.512403, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000217 DAOQVJ010000000
VERSION     DAOQVJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50318_length_2514_cov_3.512403"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="PKL26_05355"
     CDS             <1..99
                     /locus_tag="PKL26_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNS83157.1"
                     /translation="LEKLLRQYGVKELVRTGKIAILRGAKTVKSSK"
     gene            complement(146..598)
                     /locus_tag="PKL26_05360"
     CDS             complement(146..598)
                     /locus_tag="PKL26_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNS83158.1"
                     /translation="MSLQEYSKKRDFSKTPEPRGAGGEGGGVPLFVVQEHHSSHHHFD
                     FRLEHEGVLASWAVPKGVPESPGEKRLAVRTEDHPLAYADFEGSIPEGEYGAGEVKIW
                     DRGTFVLNTWEADRIEVILQGNRLNGRYLLVRFKRAGENDWLLFRAKE"
     gene            713..2026
                     /locus_tag="PKL26_05365"
     CDS             713..2026
                     /locus_tag="PKL26_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS83159.1"
                     /translation="MRTLVLGGGLTGITLAHLLRQRGQEATVLEAAERIGGLCRSRSE
                     NGFTFDVGGSHIIFSRDHEVLEFMKNVLGSNREQRTRNTRILFRDILVKYPFENGLAA
                     LPKDDLFYCINEFIQTYLKAEKGEIPPPANFREWIYATFGKGIADLYMVPYNEKIWNF
                     PPESMSAHWVEGRVPRPPVEDVIKSAIGIETEGYTHQSVFTYPAEGGIESLVKSIARG
                     IEDCILTGFTVRSLAFRGGVWEAGDGKRTVTGDRVISTIPLQHLLPCLGGGVPPAVKE
                     AVGALRYNSVCCVQIGIKGRVPDISWLYIPYRTQSLANRISFPSNFSSHVAPPGHAGI
                     LAEITYNEGDEVSRMTDDELVRHVIESLERMGFLTPDQVVHTAVDRQKFAYVVYDLAY
                     LENIRIVREYFQARGITLVGRFSTFEYLNMDGCIRQAMECVRDWA"
     gene            2023..>2514
                     /locus_tag="PKL26_05370"
     CDS             2023..>2514
                     /locus_tag="PKL26_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS83160.1"
                     /translation="MKVNFFVEDMLFFRFIGCATVAKTLYHQLQNEPGIDVSWKSFSN
                     HADIVHYHTFGPIAGLNRRMTRGKKILTAHSTPRINMGNIALARAINKRYPKIYRRFD
                     HIISISRPCHEEVTAMIPEMPVTMIPNGIDREFFRRDDEKRAAFREYYGIPDEQQVVL
                     TVAQ"
BASE COUNT          563 a          728 c          690 g          533 t
ORIGIN      
        1 ctcgagaaac tgttgcgcca gtacggggta aaggagctgg tcaggacagg gaagattgcc
       61 atactgcgcg gtgcaaagac ggtgaagagc tcaaaataga gagattcctt tttccttttt
      121 ttaattcatg cggtggatca gccattcact cctttgccct gaataacaac cagtcgttct
      181 cccccgcacg tttgaaccgg acgaggagat accgcccatt gagccggttt ccctgcaata
      241 tcacctcgat ccggtcggcc tcccaggtgt tgagaacaaa ggtgccgcgg tcccagatct
      301 tcacctctcc tgcgccgtac tctccttcgg ggatgcttcc ctcgaagtca gcatacgcga
      361 gcgggtgatc ctcggtccgg actgcgagcc gcttctcccc cggcgactcc gggacgccct
      421 ttggcaccgc ccaggatgca agcacccctt cgtgctccag gcggaagtcg aaatggtggt
      481 ggcttgaatg gtgttcctgc acgacaaaca acgggacccc gccaccttct ccgccggccc
      541 cgcgcggttc aggtgtcttc gaaaaatccc ttttctttga atattcctgg aggctcatga
      601 gttcaacccc gggttcataa cgtcgttctt tttctctccg tcaacagctt gcgcatcaga
      661 acacactcgt ggatggtcaa tcatataatc cctcccgttt aatagtatgc tcgtgcgtac
      721 actcgtcctg ggaggagggc ttaccggaat cactctcgca cacctgctgc ggcagcgtgg
      781 ccaggaggcg acagtactcg aagctgcaga aaggatagga gggctctgcc gctcccgttc
      841 agagaacggg ttcactttcg acgtcggggg atcgcatatc atcttctccc gcgaccacga
      901 ggtgctcgag ttcatgaaaa acgtcctcgg ctccaacagg gagcagcgga cgagaaatac
      961 caggattctg ttccgggata ttctcgtgaa atatccattc gagaatggtc ttgcggccct
     1021 tcccaaggac gatctcttct actgcatcaa cgagttcata cagacatacc tgaaagccga
     1081 gaagggggag atcccccctc ctgccaactt cagggaatgg atttacgcga catttggaaa
     1141 ggggattgca gatctctaca tggtccctta caatgagaaa atctggaact ttccccccga
     1201 gtccatgtct gcccactggg tcgaggggag ggttccgcgt cctcccgtgg aagacgtgat
     1261 aaaatctgcc atagggatcg agactgaagg gtatacccat cagtcggtct tcacctaccc
     1321 ggcagagggt ggaattgagt cactggtcaa atccattgca agaggaatcg aggactgtat
     1381 cctgaccgga ttcactgtcc ggtcgcttgc gttccgtggc ggagtctggg aggccggaga
     1441 cgggaaacgg acagtcacgg gagaccgggt gatcagcacc atcccgctgc agcacctcct
     1501 gccatgcctc gggggaggag tccctcccgc cgtcaaggag gcagtcggtg ccctccgcta
     1561 caactccgtc tgctgcgtcc agatcgggat aaaggggagg gttcccgaca tttcatggct
     1621 ttatatcccc tacaggacac agagcctcgc aaaccgcatc tcgtttccct ccaatttcag
     1681 cagccatgtg gcacccccag ggcacgcagg tatcctcgcc gagatcacct acaacgaggg
     1741 cgacgaggtc tcccggatga ccgacgacga acttgtccgc cacgtcatag aatccctcga
     1801 gcggatgggg ttcctcaccc cggaccaggt ggtccatacc gcggttgacc ggcagaagtt
     1861 cgcctacgtg gtatacgacc tggcatacct tgagaacatc aggattgtca gggaatattt
     1921 ccaggcgcgg gggatcacgc tggtgggaag attctcaacg ttcgagtacc tgaacatgga
     1981 cgggtgcatc cgccaggcca tggagtgcgt gagggactgg gcgtgaaggt caatttcttt
     2041 gtggaagaca tgctcttctt ccggttcata gggtgtgcga cggttgcaaa gacactctac
     2101 caccagctgc agaatgagcc tggaatagat gtctcctgga agagtttttc aaaccacgca
     2161 gacatcgtcc attatcatac gttcggcccc atcgccggcc tcaaccgccg gatgaccaga
     2221 gggaagaaga tcctgaccgc acattccact ccccggatca acatgggaaa tattgccctt
     2281 gcgcgtgcca tcaacaagag gtatccaaag atctaccgga ggttcgatca tatcatctcc
     2341 atctcccgcc cctgccacga ggaggtgacc gccatgatcc cagagatgcc ggtgacgatg
     2401 atcccaaacg gcatcgacag ggagttcttc aggagggacg acgagaagag ggcagccttc
     2461 agggagtatt atggcatacc tgatgaacag caggtcgtgc tcacggtagc acag
//
LOCUS       DAOQVJ010000218         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_50834_length_2497_cov_4.040541, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000218 DAOQVJ010000000
VERSION     DAOQVJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50834_length_2497_cov_4.040541"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKL26_05375"
     CDS             complement(<1..349)
                     /locus_tag="PKL26_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNS83161.1"
                     /translation="MELLDPARVLLASRYLLPGESPEGMCERVAHAVGTQKKRQFLEI
                     IESLQFLPNSPTLMNAGTPTGQLSACFVLPVEDSIRQIFAVLGYMALIHKSGGGTGFS
                     FSRVRPRGDSVAGT"
     gene            401..1837
                     /locus_tag="PKL26_05380"
     CDS             401..1837
                     /locus_tag="PKL26_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNS83162.1"
                     /translation="MSLMVLGTSSHAGKSTVVAALCRVLVNRGISVAPFKSQNMSLNS
                     YVTRDGCEIGIAQAMQAFAARIEPAPEMNPILLKPKGDATSQVVLMGHPYKDVPAREY
                     YLETEFLLEKAVSAARTLEERFGHLVVEGAGGAAEMNLYKRDIANILLARELQYPIIL
                     VADIERGGVFAQVYGTVRLLPEDISPLVVGIIINKFRGDPEIFSQGIEIIEEICGVPV
                     LGVLPYAGMNLPSEDSLSIAEKQSLDHPIRIAIIRLPRISNFSDFEALERDASVEYVP
                     LGSPLADYDCIILPGTKNTVDDLAEVRDSGTAEEVRRARKRGTPIIGICGGYQMLGRE
                     VIDSGIESRQGTHPGIGILDVVTRFDRYEKKTEQVTIDACPVGPVLSMMGKVSGYEIH
                     MGMTERAGAVEAFFGEGAATPDGLVFGTYIHGLFSNASAVDALLSFLYARRGISYAPR
                     IAERDPYEDLAAMFSQHVRMDLILPYFE"
     gene            1964..2302
                     /locus_tag="PKL26_05385"
     CDS             1964..2302
                     /locus_tag="PKL26_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein E"
                     /protein_id="HNS83163.1"
                     /translation="MPMRVSITIDPDTMDLVDKFGKEHGLDRNRAILECIEAGYARLT
                     DGTPVSFTQTKTFEEYNDLKKAITDVKESLSNLAEEVRLIHHTIEVEWTKEIRGVPFQ
                     SKRWYEFWKS"
BASE COUNT          570 a          668 c          736 g          523 t
ORIGIN      
        1 ccgtcccggc aacggaatca ccccggggcc gcacgcgtga aaaggagaaa ccagtgccgc
       61 cgccgctctt gtggataagc gccatgtacc caagaactgc gaagatctgg cggatggagt
      121 cctcgacagg caggacaaag cacgcagaga gctggcctgt gggggttccc gcattcatga
      181 gtgtggggga attcggtaaa aattgtaaac tctcaatgat ctcgaggaat tgacgctttt
      241 tttgggtccc caccgcgtgc gccaccctct cgcacatccc ttcgggcgac tcccccggaa
      301 gcaggtaacg gcttgccagc aacactctcg ccgggtccaa gagttccata acgcagtgag
      361 aggaatgctc tttatttaaa tccatccata ttttatgcac atgtctctga tggtgcttgg
      421 cacctcctct cacgcaggaa agagcacggt ggtggcggca ctctgccggg tgcttgtcaa
      481 cagggggatc agcgtggcac cgttcaagtc gcagaacatg agcctgaact cctatgtcac
      541 gcgtgacggg tgcgagatag gaattgcaca ggccatgcag gcttttgctg ccaggattga
      601 gccggctccg gagatgaacc caatcctcct caagccaaag ggtgatgcaa catcccaggt
      661 ggtgctgatg ggccacccat acaaggacgt ccctgccagg gagtattacc tggagacgga
      721 atttctcctt gaaaaagcag tgtctgccgc ccgtaccctg gaagagcgct tcggccacct
      781 ggtggtggag ggagcgggcg gtgcagcaga gatgaacctc tacaagaggg acatcgcaaa
      841 cattctcctc gcgagggaat tgcaataccc catcattctc gtggccgata ttgagagggg
      901 cggtgtgttt gcacaggtgt atggaacagt caggctcctc ccagaagata tctccccgct
      961 ggtggtgggc atcattatca acaagttccg gggcgacccg gagatcttct cgcagggaat
     1021 agagatcatc gaggagattt gcggggtccc tgtgctgggg gtgctgccat acgcgggcat
     1081 gaacctcccg agcgaggact cgctctcgat cgcggagaag cagtcgcttg atcaccccat
     1141 cagaattgcc attatcagac tcccacggat ctccaatttc agcgactttg aggcgcttga
     1201 gagagacgcg agtgtggaat atgtccccct gggatcgccc cttgccgact atgactgcat
     1261 catcctgccg gggaccaaga ataccgtcga tgatctcgca gaggtccggg attcagggac
     1321 agcagaggag gtacggcggg cgcggaagcg cggcactcca attattggta tatgcggggg
     1381 gtaccagatg ctcgggcggg aggttatcga ttcggggata gagtctcgcc agggaaccca
     1441 cccgggaatc ggtatccttg acgtggtgac tcggttcgac cgctatgaga agaagaccga
     1501 gcaggtcacc atcgatgcat gtccggtcgg accggtgctc tccatgatgg gaaaggtatc
     1561 gggttacgag atccacatgg gaatgacaga gcgggcaggg gcagtcgaag cgttttttgg
     1621 tgagggagcg gctacccccg atggcctggt cttcggcaca tacatacatg gcctgttctc
     1681 gaatgcgtcc gcggttgatg cgctgcttag ttttctttat gcaagacggg ggatctctta
     1741 tgcaccacgg atagcggagc gtgaccctta cgaggatttg gcagccatgt tctctcaaca
     1801 cgtgcgaatg gacctcatcc tgccttattt cgagtgattt tcacagaatg cgctggattt
     1861 tacctggcac cgggctattt ggatctcccg gctgcagaca gggagatatt tgagtgagga
     1921 gacacaaact cactcgcgaa agaagtgtgg aggaggaact accatgccca tgcgggtatc
     1981 aatcaccatc gatccggata ccatggacct tgtcgataaa ttcggcaagg aacacggcct
     2041 tgacagaaat cgcgccattc tcgaatgcat agaggctgga tatgccaggc ttacagacgg
     2101 gacacctgtc agcttcaccc agacgaagac atttgaggag tataatgacc tcaaaaaggc
     2161 tattaccgat gtgaaggaga gcctctcgaa ccttgcagaa gaggtaaggc tgatacacca
     2221 taccatcgag gtggaatgga caaaggagat acggggtgtc ccgttccaga gcaagcggtg
     2281 gtacgagttc tggaaaagct gacccctacc agccgggatg atcccttcgt cacccgatga
     2341 tgctcatcat gctccggtag ccctcccccc tcctgtcacc gcatttcacg gcttcaactg
     2401 cgctgaggtc aacctctttc gcgtcgcggt gccggaggca gtatgcgagg gctggtgact
     2461 tcacgtactc ccctttcacc cggaaatacc tggaatg
//
LOCUS       DAOQVJ010000219         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_50875_length_2496_cov_3.759934, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000219 DAOQVJ010000000
VERSION     DAOQVJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50875_length_2496_cov_3.759934"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKL26_05390"
     CDS             complement(<1..439)
                     /locus_tag="PKL26_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNS83164.1"
                     /translation="MNTHVLIFLFFLSAMAGILPVNGSQPVAGDAGYFLIESDPSGAQ
                     VIFDNRFIGVTPVTYPIMGTSSPPHTITIAASGYYPHTTQYMVNPQPGETIRAYADLE
                     PSQSFGTLVITSTPGGALVTVDGGKGQQAPWTYQDIRAGSHLVQ"
     gene            716..1132
                     /locus_tag="PKL26_05395"
     CDS             716..1132
                     /locus_tag="PKL26_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNS83165.1"
                     /translation="MLERLNTFEKYVYYVLIILLAFIIFVSVIELALLIAGGLVIDQT
                     YRLDNHEILTIFGFFLLVLIGVELLDTIKAYIKKQEIHVEIIVLLAVIAVARKIILLD
                     PYSDMPLSDMTLWGLGFIVLCLAAAYYLIKKAGVAT"
     gene            1321..2202
                     /locus_tag="PKL26_05400"
     CDS             1321..2202
                     /locus_tag="PKL26_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83166.1"
                     /translation="MQDLIPRSCRGRTLIALLTLCLLGMPAAAAVLETTYMGRITALD
                     PLSGTITIRAESQYSCDYSGSVAVCSFAPLTPMQVVGTVPDEGIFNTFRNNDPVVATI
                     LGASGGEWAGFALVAPVPGTDQWAATEVFGDPHTIPVNLAGNYEMDYSTTPDCSACTG
                     SVCKAQSAHVVLKSEGNIVLERALTAGNSARYSGRNDGSSVSILYLSGEADSGPCSPA
                     GAMAGMQPISNFIIHVVPPIGQAGTPGIVTHPTAAGIEPKVTPLATPAATQAPSGCAL
                     VVTAFCIVAVGLRRRFS"
BASE COUNT          529 a          721 c          689 g          557 t
ORIGIN      
        1 cctgtaccag gtggctgccg gcgcgtatgt cctggtacgt ccagggtgcc tgctgcccct
       61 tcccgccatc gacagtgacc agtgcaccac ccggtgtgga cgtgatcacc agggtgccga
      121 atgactggct gggttccagg tcggcgtatg ccctgatggt ctctccaggt tgtggattga
      181 ccatgtactg ggtggtgtgg gggtagtacc cgcttgcggc aatcgtgatg gtatgcgggg
      241 ggctggacgt tcccattatg gggtaggtga ctggagtaac tccaatgaag cggttgtcaa
      301 agatgacctg cgcaccagag ggatctgatt ctatcaggaa atagccagca tcgcctgcga
      361 ccggttggga tccattgaca ggaagaatgc ctgccatggc agataaaaaa aagagaaata
      421 tgagcacatg tgtgttcatt cgcatatatg cacacctctc tctcttcttc tacaaaaatt
      481 catgggcggc gccccagggg aagagcagtc caaagtcctg attaaaagac cgtccaacca
      541 ggtgtggcag agagtttata ggacgggaag gttcatgtac gagtgaacat cctgccaggc
      601 tccacgacac gtggacaacc ggcccgcgtg aatatgggtt ccgcgggtgc ggaaggaccg
      661 cagttcctgc aatgcagaag aaccgtgccc ggccccgggt gaaaagaggg aatcgatgct
      721 ggaacgactg aatacattcg agaaatacgt ctattacgtt cttatcatcc tccttgcgtt
      781 cattatcttt gtttcggtca ttgagcttgc cctcctcatt gccggcggcc tcgtcattga
      841 ccagacatac cggctggaca accacgaaat actgacgata ttcggcttct tcctcctggt
      901 gttgatcggg gtggaactcc tggacaccat caaggcatac atcaagaaac aggagatcca
      961 cgtggagatc atcgtcctcc tggcagtcat cgcggttgca aggaagatca tcctcctgga
     1021 cccgtactcg gatatgcccc tgtcggacat gaccctgtgg ggccttgggt tcatcgttct
     1081 ctgcctcgcc gcagcatatt acctgatcaa aaaagcagga gttgcgacgt aaggggacag
     1141 gcccatcctt ctcatgcgcc ggccgcattc catgccgcaa agaaaccctg tgcctcggcc
     1201 acaggctctt gttcagctct cgaatacctt caagaaggac acctgccagg ctctccattc
     1261 cactatatca tctggttttt tatttcaacc gcaccatatc tcacctcaac catgacatgc
     1321 atgcaagatc tcataccccg gtcctgcagg ggtcgcactc tcattgcgct cctcactctc
     1381 tgcctccttg ggatgcctgc cgcggccgca gtgctggaga cgacatacat ggggcgaatc
     1441 actgccctcg atccgctctc tggaaccata accatacgtg cggaatcaca gtactcctgc
     1501 gactattcgg ggtcggtcgc tgtctgcagt ttcgctcctc tcacccccat gcaggtggtg
     1561 gggactgtac ccgacgaggg aattttcaac accttccgca ataatgatcc tgtggtcgcc
     1621 acaatcctcg gagcaagcgg cggcgagtgg gcggggttcg cccttgttgc gccggttccc
     1681 ggaacggacc agtgggcagc gaccgaggta ttcggcgacc cgcacacaat ccctgtaaat
     1741 cttgcgggca actacgagat ggactattcc acaacccctg actgcagcgc atgcacagga
     1801 tcggtctgca aggctcaatc cgcgcatgtt gtcctgaaga gcgaggggaa catagtgctc
     1861 gagagagcgt tgactgccgg aaattctgca cgatattcag ggaggaacga cggttcaagc
     1921 gtgagtatcc tgtatctttc cggtgaggca gactcgggcc cctgttcacc tgcaggggcc
     1981 atggccggaa tgcagccgat ctccaatttt atcattcacg tcgtcccgcc catcgggcag
     2041 gccggaactc cggggattgt cacgcacccg actgctgcgg gaattgaacc caaggttacc
     2101 ccgctggcta cacccgccgc cacgcaggca ccctccgggt gtgccctggt cgttaccgca
     2161 ttctgcattg tggctgtggg cttaaggcga cgattcagct gaccgcctgt acactaaagt
     2221 tatcatgaac ccggtcccca taatttttcg tcagcacact tttgggacgg ctttcaatcg
     2281 agccacatcc tcccggggcc agtcatgatg ccggggcagt cccggggacg ggagtattat
     2341 cacatccggg aagtgtgcgc caggctgtca aaagccccgg gccgtggcga agctggtatc
     2401 cttatcgcaa gagaggtatc tgcatactct ctgttcgatc tccaggtgat tatgggcagg
     2461 gtcaggcatg aagtgcatcg ccttcctccc tcgtac
//
LOCUS       DAOQVJ010000220         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_50889_length_2496_cov_2.740270, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000220 DAOQVJ010000000
VERSION     DAOQVJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50889_length_2496_cov_2.740270"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKL26_05405"
     CDS             <1..470
                     /locus_tag="PKL26_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS83167.1"
                     /translation="KKGRLAVFQCGMMCGVITASDLIREMPDAPETSLVVDDFMTREV
                     VCVKDSQKIEDVARIMGRKRIGSVIVTESGAPRGIFTERDLLSTLIAKGVSLGVPAGE
                     VCSPMLVTAPAGISIHEAAKIMAAKHIRRLPLMKKKKLAGIITARDLVEAYAK"
     gene            complement(586..1035)
                     /locus_tag="PKL26_05410"
     CDS             complement(586..1035)
                     /locus_tag="PKL26_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS83168.1"
                     /translation="MAMSMEESGGHWFSQMPRTGRIYQLIDEAGKAGFRSGRVDAIVA
                     LGECRDPRAVDVLVTCCQDRDPSIRMSAVTALGRMRSGRAVPALVRCIQDRDEVQEIR
                     REAASALASTRCESAVEGLRGCLEDEKVDPAVRSLVEEALAQIRTKG"
     gene            1435..1941
                     /gene="msrA"
                     /locus_tag="PKL26_05415"
     CDS             1435..1941
                     /gene="msrA"
                     /locus_tag="PKL26_05415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNS83169.1"
                     /translation="MEEYHGPEQATFAAGCFWGVEAAFRQVKGVVATEAGYTGGTVSN
                     PTYQQVCSGKTGHAEAVRVFFDPKVVTYDQLLDIFWGIHDPTQVNRQGPDIGTSYRSA
                     IFYHSPAQKAAAEASRDRLQRSGRFGRVPVATEIAPAAEFWKAEEYHQQYFEKCGCGY
                     SATPKYWE"
     gene            complement(1975..>2496)
                     /locus_tag="PKL26_05420"
     CDS             complement(1975..>2496)
                     /locus_tag="PKL26_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNS83170.1"
                     /translation="ESAKKASDNIERQISFTKDYQDMGVKSATWQEIGLALTRARGAL
                     PIEHLSIKAEGTEYEIFADPLFEKVFYNLFENSLKHGGEGMTTISISTRETDEGLLIL
                     YEDDGVGIPENAKKFLFAQEMTPQKGLGMFLTGQILAITGITIVETGVPGRGARFEML
                     VPKGGFRKAKSGD"
BASE COUNT          511 a          758 c          699 g          528 t
ORIGIN      
        1 ggaagaaagg gcgcctcgcg gtcttccagt gcgggatgat gtgcggcgtg atcactgcat
       61 cggacctgat ccgcgagatg ccggatgccc cggagacctc gctcgttgtc gacgacttca
      121 tgacaaggga ggttgtctgc gtgaaagaca gtcagaagat cgaggacgtg gccaggatca
      181 tgggtcggaa gcgtatagga agcgtgatcg tgaccgagag cggcgcaccg cgggggatat
      241 tcaccgagcg cgacctcctg tcgacgctta tcgcgaaagg cgtctcgctg ggtgtcccgg
      301 ccggagaagt ctgctcgccg atgctcgtca ctgcgcctgc agggatcagt atccacgagg
      361 cggcgaagat catggccgca aagcatatcc gcaggctccc gctgatgaag aagaagaaac
      421 ttgcagggat catcaccgcg agagacctcg tggaggccta cgcaaagtaa acggggatat
      481 cctggggacc aggggtagcg ggacctggtc acgcaggtgt gcgtattaag atcagagaga
      541 gggaacccct cctcccacac acagcggtgt gttccacacc ccgcctcaac cttttgtccg
      601 gatctgtgcg agagcctctt ctaccaggga cctgactgcc gggtccacct tttcatcttc
      661 caggcaccct ctcagccctt cgactgcact ctcgcaccgg gtggaagcga gcgcactggc
      721 cgcttccctc ctgatctcct gcacttcgtc cctatcctgt atgcatctga caagggccgg
      781 gaccgcccga ccactcctca tcctgccgag cgccgtcacg gcactcatcc gtatggatgg
      841 atccctgtcc tggcagcagg tgacaaggac gtcgactgcc cgggggtccc tgcactcccc
      901 aagcgccacg atcgcatcaa cccttccact ccggaatccc gcttttcctg cttcgtctat
      961 cagctggtaa atccttccgg tccttggcat ctggctgaac cagtgccccc ctgattcttc
     1021 catgctcatt gccatgatat ccccacaacg agagtataca taaaaacacc aatcctggcc
     1081 ccggccggga gaacacccag cagaaaccag ttctcactta gtcgtgaaaa gtggttgccg
     1141 ctttccagca gcagaatcct gtaccgttcc cgatagcctg attgtgcgat ggagagattg
     1201 gtacattatt cccttttatt gcctcaccac gatatatagg tatccttctc ccggaggacc
     1261 ttgcagtcct ccacgagagg ggggtaccgg gggttcctga aaggcaggag atactggtag
     1321 caggagcgcg atctcgccgg ccaaaagttt tggaacatgt gggttttgcg cccggattgg
     1381 ccaagccttc ccggaccaaa acctccttct ccctgtccca cacaatcaga ctgcatggaa
     1441 gagtaccatg gccctgaaca ggccacgttc gccgccggct gcttctgggg agtcgaagcc
     1501 gcgttcaggc aggtcaaggg ggttgttgca acggaggcag gttataccgg ggggactgtc
     1561 tccaacccta cataccagca ggtctgttcc ggaaaaacag gccacgcaga ggcagtgagg
     1621 gttttctttg acccaaaagt cgtcacgtac gatcaactcc tcgatatctt ctgggggata
     1681 cacgatccca cgcaggtaaa ccgccagggg cctgacatcg ggaccagtta ccggtccgcc
     1741 atcttttatc actcgcctgc gcagaaggca gcagcagagg catcgcggga caggctgcag
     1801 cgatcgggga ggtttggaag ggtgccggtt gcaaccgaga ttgcacccgc agccgagttc
     1861 tggaaggccg aggagtatca ccagcagtac ttcgagaagt gcggctgtgg gtactctgcc
     1921 acgccgaaat actgggaata acgcgcgatt gcgggcggct gcgttttgcc ccggtcaatc
     1981 gccggatttt gctttccgga aacccccttt cggcaccagc atctcgaacc gggcccctct
     2041 gcccggaaca cccgtctcca cgatggtgat tccggtaatc gcgaggatct gcccggtaag
     2101 aaacatcccg aggcctttct gcggcgtcat ttcctgcgcg aagaggaact ttttcgcatt
     2161 ctccgggatt cccacaccgt cgtcctcgta caggatcagc agcccttcat cggtctcgcg
     2221 ggtcgagata ctgatggtag tcatcccctc ccccccgtgc ttgagggagt tctcaaagag
     2281 gttgtaaaag accttctcga agaggggatc ggcgaatatc tcatactcag tcccttctgc
     2341 cttgatcgag aggtgttcta ttggaagagc gcctcgtgcc cttgtaagcg ccaaacctat
     2401 ctcctgccat gtggcactct tcactcccat gtcctggtag tccttcgtaa agctgatctg
     2461 gcgctcgatg ttgtctgacg cctttttggc actctc
//
LOCUS       DAOQVJ010000221         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_50920_length_2495_cov_3.297541, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000221 DAOQVJ010000000
VERSION     DAOQVJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50920_length_2495_cov_3.297541"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKL26_05425"
     CDS             <1..182
                     /locus_tag="PKL26_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83171.1"
                     /translation="GHRIRLEVTSSNFPCWDRNTNSGNNPMKDTYADIRVATTQVFHD
                     RAYPSCMVIHGWEER"
     gene            179..439
                     /locus_tag="PKL26_05430"
     CDS             179..439
                     /locus_tag="PKL26_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HNS83172.1"
                     /translation="MRKSREILLRCYHDPAFVFSNVQVCYIDRGAPGNESCVPGERIH
                     ALDSQYMEIGEGPESSPIPYHRITRIMYQGEVLWERQGKHSE"
     gene            complement(723..1445)
                     /locus_tag="PKL26_05435"
     CDS             complement(723..1445)
                     /locus_tag="PKL26_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83173.1"
                     /translation="MGDLALLVLEGPWWTPAHKPKRPSVLPFLEGLEKYKGNFNIYYS
                     SFYEKESFRKALEDDLAHTREQRLFLYIAAHGGSRMVGALEGEGGKTISSGMRLTTLL
                     RGVRRVARTTNIEGVLLGSCTLGANRTDLLATLKTSPIAWIFGYACEIDWIPSTLIDL
                     SILEHAMALKKEDLRSRTKIVGAFSSALSRFSGEFPICKEENRSVPLKYAISLLAKPR
                     KPHAVPEDLTRALLGSLGWDTP"
     gene            1661..1733
                     /locus_tag="PKL26_05440"
     tRNA            1661..1733
                     /locus_tag="PKL26_05440"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1694..1696,aa:Thr,seq:tgt)
     gene            1797..>2495
                     /locus_tag="PKL26_05445"
     CDS             1797..>2495
                     /locus_tag="PKL26_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNS83174.1"
                     /translation="MSTAPLGTERSFHSIRRDSAERAITAALESHQITARDAALIREY
                     LGELKSCSGISDGRVNKNAYIMVGWRRYLGPYDSLTIGDIFAGRTAFENSRNGRGTPY
                     KQNTRHDWIKELKRFLIWLIENEYLTLPLAKVQKIRTPPTDPSTKVTGDLLTPEEIKA
                     MIRACSRSADRAIIMTLYEGGFRIGEVGRMRWGDLKFDEYGVVVNVTFKTGRPRYIRL
                     VMAREYLAQWRADYP"
BASE COUNT          522 a          734 c          744 g          495 t
ORIGIN      
        1 aggggcaccg catcaggctc gaggtgacga gcagcaactt cccctgctgg gaccgaaaca
       61 ccaactccgg aaacaaccca atgaaggaca cttatgcgga catccgggtg gcgacaaccc
      121 aggtcttcca cgaccgggct tacccttcgt gcatggtgat ccacgggtgg gaggagcggt
      181 gaggaagagc cgcgagatcc tactccggtg ctaccatgac ccggcattcg ttttttccaa
      241 cgtccaggtc tgttacatcg acaggggggc gccgggaaac gagtcatgcg ttcccggaga
      301 gagaatccat gccctcgatt cgcagtacat ggagatcggg gaaggaccgg agtcgtctcc
      361 catcccgtat caccgcatca cccgcatcat gtaccagggc gaagtgctct gggaacggca
      421 ggggaaacac agtgagtagc aggcgccctc tctcttatac gcctcgtcgc tttgccatgt
      481 gggtgacttg aggttgagcg atctttgcat acctatccag gatcgctccg gggcttcccc
      541 acgcagcgtg tatcacgaag aggggtgcgg gatgactgct ccctgcataa tgggcccggg
      601 gttcctaaat ctttacttac attcgcggag agcccgatgt acctgacgaa ctccctagag
      661 gaagggcggc aatagtcaca tcgatgcccg ttctcgccgg ggcatgggcc gtccttccgg
      721 gatcaggggg tgtcccaccc aagggaccca aggagcgccc tggtcaggtc ttcgggcacc
      781 gcgtggggct tccgcggctt ggcaagaagt gagatggcat acttcagggg gacggaccgg
      841 ttctcctcct tacagatggg aaactcgccg ctgaacctgg atagcgcgga tgagaacgcc
      901 ccgacaatct ttgtcctgct ccggaggtcc tctttcttga gcgccatggc atgctcgagg
      961 atggagaggt cgatgagcgt gctcgggatc cagtcgatct cgcaggcata cccgaagatc
     1021 caggcgatgg gggaggtctt cagggtggca aggagatcag tgcggttcgc cccgagggtg
     1081 caggagccga gcaggacccc ctcgatattc gtggtccgtg ccaccctcct gacaccccga
     1141 agcagcgtcg tgagacgcat cccgctgctg atggtctttc cgccttcgcc ctccagcgcc
     1201 ccgaccatcc tgctcccgcc gtgggctgcg atgtagagga agagcctctg ctccctggtg
     1261 tgggcgaggt cgtcttcgag cgccttccgg aagctctctt tctcgtaaaa gctcgagtag
     1321 tagatgttga agttgccctt gtacttttcg agcccttcca gaaacgggag cacggagggg
     1381 cgcttgggct tgtgggcagg cgtccaccac ggaccttcca gaacaaggag tgctaaatcg
     1441 cccattgtga atcagcactc atccggatgt gatatcaagg tttggtttgc cggattgtaa
     1501 aaaggggaat agacgaaggt aagattgcag agtgatattc gatctctcct atcgctcaga
     1561 tgttgaggtc atggaagagt aagtaggaga atcctgcctc actgtgtaac acttcttccg
     1621 tcgccatctt tttcactctc cgatggcaac agtatatggc gccccagtgg cttagctggc
     1681 agagcggctg acttgtaatc agcaggtccc gtgttcaaat cacggctggg gcttgccgca
     1741 ttcgttcaaa ggcccttgca aaaccttttt ttaccttgag ccgaaatgta gatttcatgt
     1801 ccaccgcacc gttaggaacc gagcggagct tccactcgat ccgaagggac agtgcagagc
     1861 gagcaatcac tgctgcactg gaatcgcacc agattaccgc cagggatgcc gccctcatcc
     1921 gggaatacct cggcgagctc aaatcgtgtt ccggcatcag cgacggccgg gtcaacaaga
     1981 acgcctacat catggtcggc tggaggcgct acctcggccc ctacgactcg ttaaccattg
     2041 gagacatctt cgcgggccgc acggccttcg agaactcccg gaacgggagg gggacaccct
     2101 acaagcagaa cacccgccac gactggatca aggaactcaa gcggttcctc atctggctga
     2161 tcgagaacga gtatctcacc ctcccgctgg caaaggtcca gaagatccgg acacctccca
     2221 cggacccgag caccaaggtg accggggacc tcctcactcc cgaggagatc aaggccatga
     2281 tccgggcctg cagccgcagt gccgaccggg cgattatcat gacgctctac gaaggaggtt
     2341 tccgcatcgg cgaagtcggc cggatgcggt ggggagacct caagttcgac gagtatggcg
     2401 tggtcgtcaa cgtaaccttc aagaccggga ggccgcgcta tatccgcctg gtcatggccc
     2461 gggagtatct cgcccagtgg cgggccgact atccc
//
LOCUS       DAOQVJ010000222         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_50984_length_2493_cov_4.681296, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000222 DAOQVJ010000000
VERSION     DAOQVJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50984_length_2493_cov_4.681296"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKL26_05450"
     CDS             complement(<1..481)
                     /locus_tag="PKL26_05450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843155.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HNS83175.1"
                     /translation="MPSPKRIGKIEFGLLSPKEIRTMSVRKIIWADTYDDDGFPYPQG
                     LMDLNLGVIDPGLRCKTCDQKASECPGHFGHIELAKPVIHVGYTRLIRKLLRVTCRSC
                     SRLLLSPEEIEKVVGTEDDLTGDFLSEKDIKKERLCPHCGEQQLKINFEKPTTFSEIF
                     "
     gene            complement(493..2307)
                     /gene="rpoB"
                     /locus_tag="PKL26_05455"
     CDS             complement(493..2307)
                     /gene="rpoB"
                     /locus_tag="PKL26_05455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HNS83176.1"
                     /translation="MKKARVFVDGALIGLVEDPKAMVSQVRAMRRQGVIPTEVNISHK
                     EFNNDVIIHTDRGRARRPLIVLHQGKPLISEEDVEKLRKKEVEFDVFIKKGLIELIDA
                     EEEEDLLIAINPGDATPLHTHMEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMVKQA
                     LGFGAANMKIRPDTRGHLLHYVQKPLVHTQTSDLIGSDDRPAGQNFVVAILSFEGYNI
                     EDALIFNKASIDRGLGRSHFFRTYEGEERRYPGGQVDRIEIPDEEVTGAHGAESYKNL
                     DDDGVINPETVVNEKDVLIGKTSPPRFLEEPTSDLITVEKRRDTSVTMRSNERGIVDT
                     VIITEGENSSRLVKVRTRDQRIPEVGDKFASRHGQKGVIGLITPQEDMPYTESGLTPD
                     LVINPHAIPSRMTIGHMLEMIGGKVGSLEGRRINGTAFSGEREEDLRASLKRQGFAHT
                     GREVMYDGITGKRFSADIYMGVIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVQEYVCARCGMVAMLDRKANTTRCLA
                     CGGETDIYPVEMSYAFKLLLDEMKSMGIAPRLKLEDVV"
     gene            complement(2304..>2493)
                     /locus_tag="PKL26_05460"
     CDS             complement(2304..>2493)
                     /locus_tag="PKL26_05460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107504.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HNS83177.1"
                     /translation="ARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISRGVQDEDE
                     VIRILYSLGVEHIKGDVR"
BASE COUNT          441 a          760 c          680 g          612 t
ORIGIN      
        1 cgaagatctc cgagaatgtc gtcggcttct caaagttgat cttgagctgc tgctctccgc
       61 agtgagggca cagcctctcc ttcttgatgt ctttctcgga gagaaaatct ccggtcaggt
      121 cgtcttcggt ccccaccacc ttctcgatct cttcagggga gaggaggagc cggctgcagc
      181 ttctgcaggt caccctgaga agttttcgta tcagccttgt gtagccgacg tgaatgaccg
      241 gctttgcgag ctcgatgtgc ccgaaatggc cgggacactc gctcgccttc tggtcacagg
      301 tcttgcaccg gagaccaggg tcgatcaccc cgaggttcag gtccatcagc ccctgggggt
      361 aggggaaccc gtcatcatca taggtatcag cccagatgat ctttcgcaca ctcatggtgc
      421 ggatctcctt cggggagagg agtccgaatt ctatctttcc aattctcttt gggctaggca
      481 ttactctccc tcctacacca cgtcttcgag cttgagtctt ggggcgatcc ccatgctctt
      541 catctcgtcg aggaggagct tgaacgcata cgacatttcc accgggtaga tgtccgtctc
      601 gccaccgcat gcgaggcacc tggtggtgtt ggccttccta tcgagcatgg ccaccatccc
      661 gcaccgtgca cagacgtatt cctgcacctt gtcagactcg tcaaggaggc gctccttcaa
      721 tgccatggcg gcaccatggc caatcatcac gtcacgctcc atctcgccga aacgcaagcc
      781 tccttccctt gccctccctt cggttggctg gcgggtcagc acctgcaccg gtccccttga
      841 ccgggcatgc atcttcgagg agaccatgtg gtagagtttc tggtagtaga ttacacccat
      901 gtagatatcg gccgagaacc gttttccggt gatgccgtca tacatgactt cacgcccggt
      961 gtgtgcaaaa ccctggcgct tcagcgaggc acgcaaatct tcttcacgtt cgcctgaaaa
     1021 ggcggttccg ttgatcctcc gtccttcgag tgatcccact ttgcctccga tcatctcaag
     1081 catgtgcccg attgtcatcc ggcttgggat ggcatgtgga ttgatgacga ggtcgggggt
     1141 gagcccggat tcggtatacg gcatatcctc ctggggggtg atcaggccga taaccccttt
     1201 ctgcccgtgg cgggacgcga acttgtcgcc aacctctggg atcctctggt ccctggtccg
     1261 gaccttcacc aggcgggagc tgttctctcc ttctgtaatg attaccgtat caacaatccc
     1321 gcgctcgttg cttcgcatcg ttactgaagt gtcacgccgc ttctcgacag tgatcaggtc
     1381 acttgtgggc tcctcgagga aacggggcgg ggaagtcttc cctataagga cgtctttttc
     1441 gttgaccacg gtctcggggt tgatcacgcc gtcatcatcg aggttcttgt agctctctgc
     1501 gccgtgggcg cctgtcactt cctcgtcggg gatctcgatc cggtcgacct gtccgccggg
     1561 atacctccgc tcctcacctt cgtatgtgcg gaagaagtgg gagcgcccaa ggccccggtc
     1621 gatggatgcc ttgttgaaga tgagggcatc ttcgatgttg tacccctcga acgagagaat
     1681 agccacgacg aagttctggc ctgccggccg gtcatcagag ccgatgaggt ccgaagtctg
     1741 ggtatggaca aggggtttct ggacgtaatg gaggaggtgc cccctcgtat cgggccggat
     1801 cttcatgttt gcagccccga atcccagcgc ctgcttgacc atgcctgcac ccatggtgac
     1861 ccttgggctt gcattgtgct cggggaacgg gacatgcgcg gcacctatcc ccaggataag
     1921 ggagggatct atctccatgt gagtgtggag aggagttgcg tcacccgggt tgattgcaat
     1981 caggaggtct tcttcctctt ccgcatcaat cagctcgatg agtccctttt taatgaacac
     2041 atcaaactcc acctcctttt tcctgagttt ctccacgtcc tcttccgaga tcagcggttt
     2101 gccctggtgc agaacgatca gtggccttcg tgcccttccc ctgtctgtgt gaatgatgac
     2161 atcgttgttg aattccttgt gggagatgtt cacttcggtg gggatgaccc cctggcgccg
     2221 catggcccgc acctgtgaga ccatggcctt tgggtcttcc acgagcccga tcagggcacc
     2281 gtccacaaac acccgtgcct tcttcatctc acgtccccct tgatatgctc cacgccgaga
     2341 gagtagagga tacgaatcac ctcatcttca tcctggaccc cccggctgat ctctaccatc
     2401 tgggcaaaat tctttacaag cccgcagttc ggcccctcgg gtgtctcgct cgggcagatc
     2461 cgcccccact gggtggggtg caggtcacgc gcc
//
LOCUS       DAOQVJ010000223         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_51468_length_2478_cov_3.751548, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000223 DAOQVJ010000000
VERSION     DAOQVJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51468_length_2478_cov_3.751548"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PKL26_05465"
     CDS             <1..1192
                     /locus_tag="PKL26_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNS83178.1"
                     /translation="YDVVTTDEAAACIRQNKSSFLRVSRPDAELPGVSPHDSMVYARA
                     REVFAEFRERGLLKKDNAPAMYLYRVTGNGNDFLGLGCTVSVRDYEENTIRRHELTRY
                     DKEEDRTRHIDAVNAHSGPVVLLFEDRNDTHGFLDALWNRLGKEDAVVSWDDGTVHRV
                     KCITGEGDLSTLERTFRAIPRLYIADGHHRAKSAVNVCLKRKVEGRLTPEATRFLGVI
                     FAHDRVKIHGYSRIVTDLSGLSAPEFMNLLKGVFSVTPYGEVDTRAFHIPPRHSSQGE
                     CHVMHMYLGGAWYECSRPRDGSMGRIEALDVSVLQREVLTGILGITDPRGDPRLHYLG
                     GARPIGDLENMVKNDGYAVAFSMQPVKVGTVIEIADAGGIMPPKSTWFEPKLLSGLVV
                     HTLD"
     gene            1591..2463
                     /gene="hisG"
                     /locus_tag="PKL26_05470"
     CDS             1591..2463
                     /gene="hisG"
                     /locus_tag="PKL26_05470"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNS83179.1"
                     /translation="MITIALPKGSLEEQTLLLFREADLEVKRSDRDYNPMIADSRIAK
                     VKILRPQEIPRFVEMGYFDFGISGLDWVKESGARVREVARLNYSKTGEGTVKIVVAVH
                     RDEPINDAKEIRPNSRVTTEYPELTREYFGRLGIPVQLFHSFGASEAKVPDLMDVVVD
                     LTETGTTLRRNGLKIIGQIMESYTMIIANRESFEDPAKRKAIEEIVTLLSGVMEARRQ
                     VLLSMNVPTSAMETVISALPAMKKPTVSRLHGIDFYSVQTVVHKQDVNTLIPSLKAAG
                     AEDILEIPITKIVR"
BASE COUNT          497 a          765 c          758 g          458 t
ORIGIN      
        1 ctacgacgtg gtgaccacgg acgaggctgc cgcctgcatc aggcagaaca agtcgagctt
       61 cctcagggtg agcaggcccg acgccgaact ccccggggtc tctccccatg attccatggt
      121 gtatgccagg gcacgcgagg tgttcgccga gttccgtgaa cggggcctcc tcaagaaaga
      181 caacgcccct gccatgtacc tctaccgcgt gaccgggaac gggaacgact tcctcgggct
      241 cgggtgcacg gtctccgtgc gcgactacga ggagaacacc atccggcggc acgagctgac
      301 ccggtacgac aaggaggagg accgcacacg gcacatcgac gcggtgaacg ctcacagcgg
      361 gcccgtggtg ctcctcttcg aagacaggaa cgatacccac gggttcctcg atgcgctctg
      421 gaacaggctc gggaaggagg atgccgtggt cagttgggat gacggcacgg tccatcgggt
      481 gaagtgcatc accggggagg gggacctctc cacccttgag aggaccttcc gggccatccc
      541 ccgcctctac atcgccgacg ggcaccaccg ggccaagtcc gcggtgaacg tctgcctcaa
      601 gcggaaagtg gaagggcggc tcacccccga ggctacccgg ttcctcgggg tgatctttgc
      661 ccacgaccgc gtgaagatcc acgggtacag ccggatcgtc accgacctct cgggactctc
      721 ggccccggag ttcatgaacc tcctgaaagg ggtcttctcg gtcaccccct acggcgaggt
      781 cgacacaagg gcattccaca tccccccgcg gcattcttct cagggggagt gccacgtgat
      841 gcacatgtac ctcggcggtg cgtggtacga gtgcagccgc ccgcgcgacg ggagcatggg
      901 ccggatcgag gcactggatg tctctgtcct ccagcgggag gtgctgaccg ggatccttgg
      961 aatcacggac ccgagggggg acccccgcct ccattatctc gggggtgccc ggccgatcgg
     1021 agacctcgag aacatggtga agaacgacgg gtacgcggtg gccttctcga tgcagccggt
     1081 gaaggtcggg actgtgatcg agatcgcgga tgcggggggc atcatgcccc cgaagtccac
     1141 ctggttcgaa ccgaagctcc tctctggcct cgtggtccat accctggact gagtatcttc
     1201 tcgcttcggc gacgtttgtt ccaaaactgc ctttcacggt ctttttttaa gaaaaagtca
     1261 ccccttgaca ggaaggatga aaatccattc tgcctgcccg tttcccgggt catatgagaa
     1321 caatggccgc ccctgtcgcg ggaatctcct ccgatccctg atgcgttata ctccaaatca
     1381 ggactggtcc ggcacacctt gcgtatcatc tttctgtggc tcatctctct atctcggtgc
     1441 tcccggggat cccggccaat ttcagtccct gcgcggcacc actccatatc aacaggttca
     1501 tggaagggcc tgcattaacg agaaggattt ctcttcgttg gtattgcaca gaggcggatt
     1561 tttttcctga acggccttat atggtatact atgatcacta tcgcacttcc caagggcagc
     1621 ctggaggagc agaccctcct tctcttccgc gaggcggatc tcgaggtgaa gcggtccgac
     1681 cgtgactaca acccaatgat cgcggacagc cgcatcgcca aggtgaagat tctccgcccc
     1741 caggagatcc cccggttcgt ggagatgggc tacttcgact tcgggatatc ggggcttgac
     1801 tgggtgaagg agagcggtgc cagggtccgc gaggtggccc ggctcaatta cagcaagacc
     1861 ggcgagggga cggtcaagat cgtcgtggca gtgcaccggg acgagccgat caacgacgca
     1921 aaggagatcc gtccgaacag ccgtgtcacg acagagtacc ccgagctgac ccgcgagtac
     1981 ttcggccggc tcggtatccc cgtccagctc ttccactcct tcggggcgtc ggaggccaag
     2041 gtgcccgacc tgatggacgt ggtcgtggac ctgaccgaga ccgggacgac gctgcggagg
     2101 aacggcctga agatcatcgg gcagatcatg gagtcctaca cgatgatcat cgcgaaccgc
     2161 gagtcattcg aggacccggc gaagcgaaag gcgatagagg agatcgtgac cctcctctcc
     2221 ggggtgatgg aggcgaggcg gcaggtgctc ctctcgatga acgtgccgac ctcggcgatg
     2281 gagacggtga tttccgccct cccggccatg aagaagccca ccgtgagccg gctgcacggg
     2341 atcgacttct acagcgtgca gaccgtggtc cacaagcagg acgtgaacac tctcattccc
     2401 agcctgaagg ctgccggggc agaggacatc ctcgaaattc ctattacaaa aatcgtccgt
     2461 taaacagccc atattata
//
LOCUS       DAOQVJ010000224         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_51718_length_2470_cov_2.984679, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000224 DAOQVJ010000000
VERSION     DAOQVJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51718_length_2470_cov_2.984679"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PKL26_05475"
     CDS             <1..1335
                     /locus_tag="PKL26_05475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNS83180.1"
                     /translation="RAGDRIGFVQEKIFRHYCMVPFSWEGFFEVISVEKAGKYTKNQA
                     IAHVKDEKGNVRPVTLCHRWPVKIPIRAYAEKLKPTEPLVTKMRLIDSIFPVARGGTY
                     CIPGPFGAGKTVLQQLISRHADADVVVIAACGERAGEVVETIKTFPTLIDPRTSRPLS
                     DRTVIICNTSSMPVAARESSVYTAVTIAEYYRQMGLHVLLLADSTSRWAQAMREMSGR
                     LEEIPGEEAFPAYLESHIASFYERAGLVRLHDGSLGSVTVGGAVSPAGGNFEEPVTQA
                     TLKVVGAFHGLSRSRSDARRYPAIDPLISWSKYGSFIDREQRETGRWLLRKGNDVAQM
                     MKVVGEEGTSLADYTVYLKSEFFDFVYLQQNAFDPVDEATQKERQIYIFDFIYQIIKS
                     DFSFKNKDEALHFFQQLRQLFRGWNSAPWQSEEFERTEQEIRGLLSERMKTG"
     gene            1337..>2470
                     /locus_tag="PKL26_05480"
     CDS             1337..>2470
                     /locus_tag="PKL26_05480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNS83181.1"
                     /translation="MQKFYSNITQIVGDVITVEAENVGNMELAQVTTRRGTSLAQVIR
                     LDGKKVFLQVFAGSRGISTGDKVRFLGHTMQIPTGDALLGRIFNGSGSPLDRGPALDE
                     NLVEVGGPPVNPVKRIIPDKMIRTGIPMIDVFNSLVESQKLPIFSIAGEPYNELLARI
                     AIQAEVDIIILGGMGLKYDDFIFFRETLEEHGALSRSIVFMHTAADPIVECLLVPDIS
                     LAVAENFALAGKRVLVLLTDMSNFCDALKVISITMEQIPSNRGYPGDLYSQLAARYEK
                     AVDFDTAGSITILAATTMPGDDVTHPIPDNTGYITEGQFYLKNSVIEPFGSLSRLKQQ
                     VNGKTRDDHRTIMDTMIQLFAEYRETLEKQAMGFEMSAWDRKLL"
BASE COUNT          559 a          698 c          734 g          479 t
ORIGIN      
        1 cgtgccgggg acaggatcgg gttcgtgcag gagaagatat tcaggcacta ctgcatggtg
       61 cccttttcat gggagggttt ttttgaagtc atctcggtcg aaaaagccgg aaagtacacg
      121 aagaaccagg caatcgccca tgtgaaggat gagaaaggga acgtgcgccc cgtaacgctc
      181 tgccatcgat ggcctgtgaa gatacccatc cgggcatatg ccgagaagct caagcccacg
      241 gaaccgctcg tcaccaagat gcgcctcatt gattcaatct tccctgtcgc acgtggtggg
      301 acatactgca tccctggtcc tttcggtgca ggaaagactg tactgcagca gctgatcagc
      361 aggcacgcag atgccgatgt ggtggtcatc gcagcctgcg gcgagcgtgc cggggaggta
      421 gtcgagacta tcaagacatt ccccacgctg atcgatccgc ggaccagccg cccactgagc
      481 gaccgcaccg tcatcatctg caacacgagc tcgatgcccg ttgcggcaag agagtccagc
      541 gtgtacacgg cggtcaccat cgcggaatat taccgccaga tgggcctcca tgtcctgctg
      601 cttgccgatt ccacctcccg ctgggcccag gccatgcggg agatgtcagg aaggctcgag
      661 gagatccccg gggaagaggc gttccctgcg tacctcgagt cccatatcgc ttcgttctac
      721 gaaagggcag ggctggtgag gcttcatgac ggatccctcg ggtcagtgac tgtagggggt
      781 gcggtgagtc ccgcaggagg aaacttcgag gagcccgtta cccaggcgac actcaaagtg
      841 gtcggcgcgt tccacggtct ctcccgcagc cgctccgacg cccgccgtta cccggccatc
      901 gacccgctca tcagctggag caagtacggg agtttcatcg accgggagca gagggagacg
      961 ggcaggtggc ttctccgcaa aggcaacgat gtcgcacaga tgatgaaagt ggtgggagag
     1021 gaagggactt cccttgccga ttacacggtt tacctgaagt cggaattctt tgactttgtc
     1081 tatctccagc agaacgcctt cgaccctgtg gatgaggcga cccagaaaga gaggcagatc
     1141 tacatcttcg attttattta ccagataata aaatcggatt tctccttcaa aaataaggac
     1201 gaggcgcttc atttcttcca gcagctccgg cagctcttca ggggttggaa ctctgccccc
     1261 tggcagagcg aagaatttga gagaactgaa caggagatcc gtgggctcct ttccgaacgg
     1321 atgaagacgg gatagcatgc agaaatttta ttcgaacatc acccagatcg tcggcgatgt
     1381 gatcacggtc gaggcagaga acgtcggcaa catggagctt gcccaggtga ccacccggcg
     1441 cgggacctct cttgcgcagg tgatacgact cgatgggaag aaagtgttcc tgcaggtgtt
     1501 cgcgggaagc cgtgggatct ccaccgggga caaagtgcgg ttcctgggcc acacgatgca
     1561 gatacccacc ggggacgcgc tccttggccg catcttcaac gggagcggga gtcccctgga
     1621 caggggaccg gcactcgatg agaacctggt ggaggtaggc ggacccccgg tgaacccggt
     1681 gaagaggatc atcccggaca agatgatccg caccggtatc ccgatgatag acgtcttcaa
     1741 ctctcttgta gaatcgcaga agcttcccat cttctccatt gcaggggagc catacaacga
     1801 gctccttgcc cggattgcaa tccaggccga ggtcgatata atcatccttg gcggaatggg
     1861 gctcaagtat gacgatttta tcttcttcag ggagacgctc gaagagcacg gtgcactctc
     1921 gcgttccatc gtgttcatgc ataccgccgc agatccaatc gtcgagtgcc tgctcgtccc
     1981 cgatatcagc ctcgcggtgg ccgaaaactt tgccctcgcg ggaaagaggg tgctcgttct
     2041 gctgacggac atgagcaact tctgcgatgc actcaaggtt atctcgatca cgatggagca
     2101 gatcccatcg aaccgtgggt acccgggcga cctgtacagc cagctcgcgg cgcggtacga
     2161 aaaggcagtg gatttcgaca ctgcagggtc aatcaccatc ctcgccgcga ccacgatgcc
     2221 gggcgacgat gtgacgcacc cgataccaga caacacgggg tacatcaccg aggggcagtt
     2281 ctatctcaag aacagcgtca ttgagccgtt tgggtccctg agccgcttaa aacagcaggt
     2341 caacgggaag acccgggacg accacaggac gatcatggac accatgatcc agctctttgc
     2401 cgagtaccgg gagactcttg agaagcaggc aatgggcttc gagatgagcg cctgggaccg
     2461 gaaactcttg
//
LOCUS       DAOQVJ010000225         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_51837_length_2467_cov_3.162106, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000225 DAOQVJ010000000
VERSION     DAOQVJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51837_length_2467_cov_3.162106"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKL26_05485"
     CDS             <1..567
                     /locus_tag="PKL26_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS83182.1"
                     /translation="RGRGFPSIPGAAGKERAMGDPVLEGLTDTLVNGTMAERREAVRR
                     LAGRGEGAIQPLLALLRQPVGNDARWYAAVALVKIGPAAIGPLIEAVAGNDDQDFRRY
                     VAAALGHLGEPAVAPLIEAMRDADPKMRGFLAQALCRIGGPAIPAVRSMLGSDDPVVR
                     ECATLTLWQMGEEGIASLLSSVDEESLQ"
     gene            679..>2467
                     /gene="polC"
                     /locus_tag="PKL26_05490"
     CDS             679..>2467
                     /gene="polC"
                     /locus_tag="PKL26_05490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HNS83183.1"
                     /translation="MMQCSPGMERYLAGLQAGLEAAMAVAEKARAAGIDPRTTVEIPV
                     ASDLADRVEALLGIKGLAKRIREMEGEMSREEVALHIGEDFVQRRFGEETDEEILDHA
                     IRTAMAVLTEGVVAAPTEGIAKVGIGRNDDGSRYLKIYYAGPIRSAGGTAQALSVLVG
                     DFVRRALGMNRYVPRQEEVERYIEEIRQYNSIMNLQYLPSEREIRLIVQNCPVCIDGE
                     GTEQEEVSGYRNLDRVETNTVRGGMALVLAEGLALKAPKVQKNVRALSLDGWDWLDEL
                     IGGAAKGGEGDDAAGIRPKDKYLRDLIGGRPVFSYPMRKGGFRLRYGRSRNTGFAAAG
                     LNPATMHILGDFLAVGTQMKIERPGKAAGIVPVDSIQGPTVRLKSGDVLRVDDAVQAR
                     ECAPEVDYILDIGEILIGYGEFLENNHPLVPPAYCHEWWCLEGGAGRPGSEEEAIALC
                     FEGAFLHPDFTWLWDDLSCTDIAELADFVSRTGELRHGILVLPPDERFKGMLEEILLP
                     HRVREGLVCISDTYVFLACLGLDLKLKKRPAWKDAPMDDPLSLVSHLSGFRMRSRGGT
                     RIGGRMGRPGKSRPRKMNPPPHSLFPLGEA"
BASE COUNT          442 a          754 c          880 g          391 t
ORIGIN      
        1 cggggacggg gctttccctc aatcccgggt gcggcgggga aggagagggc catgggtgac
       61 ccggtgcttg agggactcac tgataccctc gtaaacggca cgatggcgga acgccgcgag
      121 gcagttcggc ggcttgccgg caggggtgag ggcgccatcc agcccctgct cgcactcctc
      181 cgccagcccg tcggaaacga tgcacgctgg tacgccgccg ttgcgctcgt gaagatcggg
      241 cccgctgcca tcgggcctct catcgaagcg gtcgcaggca acgatgacca ggacttccgg
      301 cgatatgtcg ctgctgcgct tggccacctc ggcgagccgg cagtggcacc gctgatcgag
      361 gccatgcggg atgccgatcc gaagatgcgg ggattcctgg cgcaggcgct ctgcaggatc
      421 ggcggtccgg cgatccccgc agtccggtcg atgctcgggt ccgacgaccc tgtggtgagg
      481 gagtgtgcga cactcaccct ctggcagatg ggcgaggagg ggatcgcgtc tctcctctcc
      541 tcggtggacg aagagtctct gcaatagcgg tcccccgcga atccgggctg cgtcagatgg
      601 catctgcaac gtgaaatgcg ggcgtctcct gcactatatc ccaactgata agtccctgcc
      661 gcggcaagag agatggtgat gatgcagtgc tctcccggga tggagcggta cctcgcgggg
      721 ctccaggcgg ggctggaggc ggccatggct gtcgcggaga aggcgagggc ggcggggatc
      781 gacccccgca ccactgtcga gatccccgtg gcaagcgacc ttgcggaccg ggtcgaggcg
      841 ctgctcggga taaagggcct ggcaaagagg atccgggaga tggaggggga gatgtcccgc
      901 gaggaggtgg ccctccacat cggcgaggac ttcgtgcagc gcaggttcgg ggaggagacc
      961 gacgaggaga tcctcgacca tgccatccgg accgcgatgg cggtcctgac ggaaggcgtg
     1021 gtcgcggccc cgacagaggg gatcgcaaag gtgggcatcg gcagaaacga cgacggcagc
     1081 cggtacctga agatctatta tgcagggccg atcaggagtg ccggggggac cgcccaggca
     1141 ctctctgtgc tcgtgggcga ctttgtccgt cgggcgctcg ggatgaaccg ctacgtcccg
     1201 aggcaggagg aggtggagcg ttacatcgag gagatacgac agtacaactc catcatgaac
     1261 ctgcagtacc tcccttcgga gcgcgagatc cggctgatcg tccagaactg cccggtctgt
     1321 atcgacgggg agggaacgga gcaggaggag gtcagcggct accggaacct cgaccgggtg
     1381 gagacgaata ccgtgcgggg cggcatggcg ctcgtcctcg cggaaggcct cgccctcaag
     1441 gcaccgaaag tccagaagaa cgtcagggcg ctatccctgg acgggtggga ctggctggac
     1501 gagctgatag gcggcgccgc gaagggtggc gagggtgacg atgcggccgg catcaggcca
     1561 aaggacaagt acctccggga cctcatcggc ggccggcccg tattctccta cccgatgagg
     1621 aaaggagggt tccggctccg ctacggccgg tcccggaaca ccggctttgc cgcggcggga
     1681 ctgaacccgg cgacgatgca tatccttggg gacttcctgg ccgtgggcac ccagatgaag
     1741 atcgagcggc ccgggaaggc tgccgggatc gtccccgtgg acagcatcca gggccctacg
     1801 gtcaggctca aatcgggaga cgtgctcagg gtcgacgatg cggtgcaggc aagggagtgt
     1861 gcccccgagg tggattatat cctcgatatc ggggagatcc tgatcggcta cggggagttc
     1921 ctggagaaca accatcccct tgtccccccc gcctactgcc acgagtggtg gtgcctggag
     1981 gggggggccg gccggcccgg gagcgaggag gaggcgatcg cactctgctt cgagggggcc
     2041 ttcctccacc ccgacttcac ctggctctgg gacgacctct cctgcactga catcgcggaa
     2101 ctggccgact ttgtctcgcg aacaggggaa ctgaggcacg gcatcctggt cctgcctcca
     2161 gacgagcggt tcaaggggat gctcgaggag atcctgctcc cccaccgcgt aagggagggg
     2221 ctcgtctgca tcagcgacac gtatgtcttc cttgcatgcc ttggcctcga cctgaagctg
     2281 aagaagcggc ccgcgtggaa ggacgcaccg atggacgacc ccctctctct cgtcagccac
     2341 ctatcaggct ttaggatgcg ctccaggggc ggcactcgta tcggggggcg catgggccgg
     2401 cctggcaaat caaggccccg gaagatgaat ccccctcccc actcgctctt ccccctgggc
     2461 gaggcgg
//
LOCUS       DAOQVJ010000226         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_51939_length_2464_cov_4.125778, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000226 DAOQVJ010000000
VERSION     DAOQVJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51939_length_2464_cov_4.125778"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKL26_05495"
     CDS             <1..726
                     /locus_tag="PKL26_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNS83184.1"
                     /translation="EVLVVGGGPYQGAPYLAGLGALRAGADIVRIASPVFEPVPDLIY
                     ERLEGKRIGARHVETLKALAERADVVVMGNGLGDKSHGVMVELARSCRRAVVDADAIR
                     TPLPQAEDTIYTPHAGEFARMTGALPPEELVPRARAVRDAAHIGTILLKGRVDVISDG
                     ERVRFNRTGSPLMTVGGTGDVLAGVAAALFCHLPAFESACLAAYVNGRAGMAVEKAFG
                     GGMLPTDLVDRIPLELFRRNEPT"
     gene            723..1208
                     /gene="moaC"
                     /locus_tag="PKL26_05500"
     CDS             723..1208
                     /gene="moaC"
                     /locus_tag="PKL26_05500"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNS83185.1"
                     /translation="MTEFTHIGDDRARMVDIGGKGEVYREARASGRILLRRETLSAIR
                     EGTVVKGNVLATARVAATLAVKETPRLIPMCHPIPISSVDVEFNILEDSVEAVIRVRS
                     HGRTGVEMEAITGVSVALLTVWDMVKSAEKDAGGQYPVTCITDIRVLEKRKGESGGSE
                     K"
     gene            1337..1927
                     /locus_tag="PKL26_05505"
     CDS             1337..1927
                     /locus_tag="PKL26_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HNS83186.1"
                     /translation="MAKSMYAYVREAWRRPSESEVKALLWNRMQQWRREGSVVRIERP
                     TRIDRARNLGYKAKQGIVMVRVKVRRGGRRKSRYVRARRSIRMGVNRITAGKSLQRIA
                     EERASRKYPNMEALNSYWVGQDGRQKWYEVILVDGNHPAIRTDSQLAWMSDHVHRGRA
                     ERGKTSAGMKGRGMGTRGKGTEKTRPSIRSHGNLGK"
     gene            1978..>2464
                     /locus_tag="PKL26_05510"
     CDS             1978..>2464
                     /locus_tag="PKL26_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HNS83187.1"
                     /translation="MYPYPLGDSTVRRMAQEAGELGFDSMVAVGARGCTYKGVEVVGG
                     TIIQGRDPREVQAAMRRIDGNPGILMATAGDYTLNRALLHVGRVRVLQGIHATPKNSF
                     DHILSKMAAERQVAVDLDLSLLVSGRDYARQKALQRYADLAGLQSRFGFPLTLSSGAR
                     SI"
BASE COUNT          515 a          672 c          863 g          414 t
ORIGIN      
        1 gaggtgctcg tggtcggggg aggcccctac cagggggctc cctatctcgc cgggctcggg
       61 gcgctccggg ccggggcgga tatcgtgagg atagcctcac cagtctttga acccgttcct
      121 gacctcatct acgagcggct cgaggggaag cggattggcg cccggcacgt cgaaaccctg
      181 aaggccctcg cggagagggc cgacgtggtg gtgatgggaa acggcctggg cgacaagagc
      241 cacggggtga tggtcgagct tgcccgaagc tgcaggaggg ccgtggtgga tgcagacgcg
      301 atcaggaccc ctctcccgca ggcagaggat accatttata ccccccatgc aggcgaattt
      361 gcgcgcatga ccggcgcttt accacccgaa gaactggttc cccgggcaag agcagtcagg
      421 gatgcggcac acatcggtac catcctgctg aaggggaggg tcgatgtcat ttctgacggg
      481 gagcgggtca ggttcaaccg gacgggctcg cccctgatga cggtcggagg aacgggggac
      541 gtcctggcgg gggtggccgc agccctcttc tgccacctgc ccgcattcga gtccgcgtgc
      601 ctggctgcat acgtgaacgg gagagccgga atggcggttg agaaggcgtt tggcggggga
      661 atgctcccga cagacctggt cgaccgtatc ccgctggaac tcttcaggag gaatgaaccc
      721 acatgacgga atttacgcac attggagatg acagggcccg gatggtggac atcgggggga
      781 aaggagaagt ataccgtgag gcgcgggcat cagggaggat cctcctccgg cgggagacac
      841 tctctgccat cagggaaggg accgtggtga agggcaacgt acttgcgacc gcgagggtcg
      901 ccgccaccct ggcagtgaag gagactcccc gcctgatccc catgtgccat cccattccca
      961 tcagcagcgt ggacgtggag ttcaacatcc tggaggactc ggttgaggca gtgatcagag
     1021 tacgttcgca tgggaggacg ggtgtcgaga tggaggccat cacgggggta tcagtagccc
     1081 tcctcactgt gtgggacatg gtgaagtccg cggagaaaga cgcaggcggc cagtatcccg
     1141 tcacctgcat caccgatatc agggtgctcg agaagaggaa gggggagagc ggtggcagcg
     1201 agaagtaaaa agtgcacggg tagcgctgac cgcgcagttt aatatccctg caggcgactt
     1261 ctataggtct tacgggatac gtcccgtgaa ggaatgtggc tcgtctttcg agctgaacct
     1321 gaaaggtgca agaaccatgg caaaatcaat gtacgcttac gtcagggagg cgtggaggag
     1381 accgagcgag agcgaggtca aggcactgct ctggaaccgc atgcagcagt ggcgccgcga
     1441 ggggagcgtg gtacggatag agcgccccac gcgtatcgac cgtgcaagga accttggcta
     1501 caaggccaag cagggcatcg tgatggtgag ggtgaaggtc cgccggggag gaaggcgcaa
     1561 gtcccggtac gtccgggccc gcaggtccat caggatgggg gtcaaccgca tcactgccgg
     1621 aaagagcctg cagcgtatcg ccgaggagcg cgcatcgcgc aagtacccga acatggaggc
     1681 gctgaactcc tactgggtgg gtcaggatgg ccgccagaaa tggtacgagg tcatcctggt
     1741 cgacgggaac cacccggcga tccggactga ctcacagctt gcgtggatga gcgaccacgt
     1801 ccaccggggg cgtgcagaac gcggcaagac cagcgcgggg atgaagggcc gcggcatggg
     1861 gacccgcggg aaagggaccg agaagaccag gccgagcatc cgttctcatg gtaacctggg
     1921 taaataactt atcctcttcc caagatcttt tttagtatgc gatgcactga cgcctgcgtg
     1981 tacccctatc ccctcgggga ctccacggtc cgccgcatgg cacaagaggc tggggagctc
     2041 gggtttgaca gcatggtggc agttggcgca aggggctgta cctataaggg agtggaggtg
     2101 gtgggcggca ccatcatcca gggaagggat ccaagggagg tgcaggccgc gatgcggaga
     2161 atcgacggga atcctgggat tcttatggcc accgcggggg attacacgct taacagggca
     2221 ctcctgcacg tagggagggt cagggtgtta caggggatcc atgcaactcc gaaaaattcg
     2281 ttcgaccata tcctctcaaa aatggccgcg gaacgccagg tggcagtgga cctggacctc
     2341 tctttgctgg tttccgggag ggattatgcc aggcagaagg cgctccagag atacgctgac
     2401 ctggccgggc tccagtcccg cttcgggttc ccgctcaccc tctcgtccgg tgcgaggtcg
     2461 atcc
//
LOCUS       DAOQVJ010000227         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52082_length_2459_cov_4.670965, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000227 DAOQVJ010000000
VERSION     DAOQVJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52082_length_2459_cov_4.670965"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1315
                     /locus_tag="PKL26_05515"
     CDS             62..1315
                     /locus_tag="PKL26_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS83188.1"
                     /translation="MPIQELAVSNFKSFRHLEIQPDNFNVIIGSNASGKSNFIQVIKF
                     IRDIAKYGLSNAISLQGGVEYLRNTRIGAGEPFSINIRYLLEEQKTDIVAMSGGFPVS
                     FRPREICYAFSLAFPDSGETFEIASDALEVEGEFFRQEEGEQRSAGTGTIALVRAGGP
                     IQYCICPPKGLELREEELFPYYFQRVDIPEGTLLMTLPLFVPGIPSLEWIFRGMKVFD
                     FDPRLLKKAVPIMGKTDLEKDGSNLAIVIKRILEDPGKKETFTRLLRDVLPFVDDVAV
                     EKFMDMSLFLKLREVYSREKYLPASMISDGTINICALLIALYFEERPVTIIEEPERNI
                     HPYLISRLVEMLKDASRSKQVIVTTHNPEMVKHVDIHDILLISRDSEGFSRLIRPANK
                     KEIRAFLEDEIGIEELFIQNMLGIE"
     gene            1312..1899
                     /locus_tag="PKL26_05520"
     CDS             1312..1899
                     /locus_tag="PKL26_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83189.1"
                     /translation="MTERRLYILVEGNDDERFFGRIILPLISHRYRSVRLIKYACLKS
                     ARVCKFIQSIRRTGDEYLVVTDIDQAPGVMAKKQVILDRFCVVNPGAVMVIIQEIESW
                     YLAGLGPSDARILGVSVPDSTDHLTKEEFNRTIPSQYVSRIAYMLEILSRFSIPEACR
                     KNRSFQYFMGRHHIAPPARSLPETGELKDQGEPES"
     gene            1966..>2459
                     /locus_tag="PKL26_05525"
     CDS             1966..>2459
                     /locus_tag="PKL26_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNS83190.1"
                     /translation="MWKHRIPAILEQKNFWTVILVISTFFAFVLNFYSLERDLTVVTP
                     HLFYIPIVIAAYWFPRRGVIYTVAIAMGYLAMAYIEGYPDIEILTQSTARFYVFVAIG
                     VIVASLSNNLKEQEDRYHGIFDYSEAGVFLAMNLRHGFVIEEVNERGANLLGYRAREL
                     VGRTL"
BASE COUNT          570 a          683 c          668 g          538 t
ORIGIN      
        1 gcaatccccg gtcatctccc ttggataaat accatatggt ggaggagtct aaccctatct
       61 catgcccatc caggagcttg cagtctccaa cttcaagagt ttccggcacc ttgaaatcca
      121 gccggacaac ttcaatgtca tcatcggttc caatgcatcg gggaaatcaa acttcataca
      181 ggtcatcaag ttcatccgcg acatcgccaa gtacgggctc tccaacgcaa tctctctcca
      241 gggaggagtc gaatacctgc gaaatacccg cattggtgcc ggagaaccat tctccatcaa
      301 tatcaggtac ttgctagagg agcagaagac agatatcgtg gcgatgagcg gggggttccc
      361 ggtctccttc aggcccaggg aaatatgcta tgcattctct cttgcgtttc ccgactcggg
      421 agagacgttc gagattgcgt ccgatgctct ggaggtcgaa ggagagttct tccgccagga
      481 agagggggag cagaggagtg caggaaccgg aaccatcgcg cttgtccgtg ccggtggccc
      541 tatccagtat tgcatctgcc cgccaaaggg cctcgagctc cgggaagaag agctctttcc
      601 ctactacttt cagagggtgg atattccgga gggaacattg ctcatgacgc tccccctctt
      661 tgttcccggc ataccctcgc tcgaatggat attcagaggg atgaaggtgt ttgactttga
      721 tccccgcctt ctcaagaagg cggtgcccat catgggcaag accgatctcg agaaggacgg
      781 gagcaacctt gccatcgtga tcaagaggat tctcgaggac ccgggaaaga aggagacctt
      841 cacaaggctt cttcgggacg tgctcccctt cgtcgacgac gtggcggtcg agaagttcat
      901 ggacatgtcc ctctttttga aattgcggga ggtctacagc agggagaagt atctcccggc
      961 gtccatgatc tcggacggga ccatcaatat ctgcgccctc cttatcgccc tctactttga
     1021 ggagcggccg gtcaccatca tcgaagagcc ggaacgtaac atccacccgt atctcatatc
     1081 ccgactggtg gagatgctca aggacgcctc gcggtcaaag caggtcattg tcaccaccca
     1141 caacccggag atggtcaagc acgtggatat ccacgatatc ctcctcatca gccgggacag
     1201 cgagggcttc tcccggctta tcaggcctgc caacaagaag gagatccggg cgttcctgga
     1261 ggatgagatt ggcatagagg agctcttcat ccagaacatg ttagggattg aatgaccgaa
     1321 cgccgtctgt acatcctcgt tgaggggaat gatgacgaga ggttctttgg caggattatc
     1381 ctccctctca tctcacaccg gtaccgctcg gtcagactca tcaagtatgc ctgcctgaag
     1441 agcgcaaggg tatgcaagtt catccagagt atccgcagga cgggagacga gtacctggtg
     1501 gtcacagata tcgatcaggc accaggcgta atggccaaga agcaggtcat ccttgacagg
     1561 ttctgcgtcg taaacccagg cgcggtaatg gtcatcatcc aggagatcga gagctggtac
     1621 cttgccgggc tcgggccttc ggacgcacgc atactcggtg taagcgtgcc cgacagcacc
     1681 gaccacctga caaaagagga gtttaaccgg accatccctt cccagtatgt ctcgaggatt
     1741 gcatacatgc tggagatcct ttccaggttc tctattcccg aggcatgccg gaagaacagg
     1801 tccttccaat actttatggg tcgccatcac attgcgccgc ctgcccgttc actccccgaa
     1861 accggggaac tgaaggatca gggagagccg gaatcctgat ggcacctttt atcatggtga
     1921 gcacccaccg aatgtgtatc taaaaggggt gaaagagagg tgggggtgtg gaaacatagg
     1981 ataccggcaa tcctcgagca gaaaaatttc tggacagtaa tccttgtcat cagcacattc
     2041 tttgcattcg tcctgaattt ttacagcctc gagagagacc tcaccgtcgt cactccgcac
     2101 ctcttctaca tcccaatcgt tatcgcggct tactggttcc cgagacgggg agtcatttat
     2161 accgtggcaa ttgccatggg ctacctggcg atggcgtaca tcgagggata ccccgacatt
     2221 gagatcctca cccagtcaac cgcacggttc tacgtcttcg tcgccattgg ggtcattgtc
     2281 gcctcgctct cgaacaatct caaagagcag gaggaccgct accacgggat cttcgattat
     2341 tcggaggcgg gggtcttcct ggcgatgaac ctccgtcacg ggttcgtgat cgaggaagta
     2401 aacgagcgcg gcgccaatct cctcgggtac agggcgcgtg aactggttgg aaggacgct
//
LOCUS       DAOQVJ010000228         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52234_length_2454_cov_5.401000, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000228 DAOQVJ010000000
VERSION     DAOQVJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52234_length_2454_cov_5.401000"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..845)
                     /gene="cbiQ"
                     /locus_tag="PKL26_05530"
     CDS             complement(57..845)
                     /gene="cbiQ"
                     /locus_tag="PKL26_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNS83191.1"
                     /translation="MYEELLEDIAQQNRLREVNTYTKLAAGLGAILLCLLSSSYVAPL
                     FIALVFAGALIFIARIDPAIYAKAFVAPLLFAGLGAGAIILLAGGGEIFWSWQIFPWL
                     SLSVTRESINQGVFVFFRVVGGTSALLFIAFSTPMTDLFQVMRRARVPEEVLDLAMII
                     YRTIFLIMDHLVMVYQAQMMRLGYRTFRESIRSFATLAGAVFIASWEAGEDLTRAMEA
                     RCYEGKFAVLGEGRPFSLPSVLAVISFLFMSAGVVAATTHVTLI"
     gene            complement(874..1179)
                     /locus_tag="PKL26_05535"
     CDS             complement(874..1179)
                     /locus_tag="PKL26_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transport protein CbiN"
                     /protein_id="HNS83192.1"
                     /translation="MWAKYRLEIIAVAAIVCFCGVFLYTSSLNADAEYAGSDTLGSSR
                     AAELAGISEEDFQPLVPQWEPPSGEIESGLFALQAAVGGIIVGWVFGFWRGQKNRST"
     gene            complement(1179..1871)
                     /locus_tag="PKL26_05540"
     CDS             complement(1179..1871)
                     /locus_tag="PKL26_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNS83193.1"
                     /translation="MHIMEGFLPLQWAVFWWVLALPCLAFGVLQLKRILGQDREALPL
                     LGVTGGFIFILSSLKLPSVTGSCSHPTGTGISSVCFGPWVTSVICAIVLLFQSLFLAH
                     GGLSVLGANIISMGVVGPLAGWAIYRALRDTGVNMYFVVFFACAIADLCTYIVTSLEL
                     ALAYPAEVGGFAGSFVLFMGIFAITQVPLAIVEGVVLALVFKYIVHLRPEILIRLKVF
                     PEEKIRAAAGDA"
     gene            complement(1972..>2454)
                     /locus_tag="PKL26_05545"
     CDS             complement(1972..>2454)
                     /locus_tag="PKL26_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HNS83194.1"
                     /translation="VGIGFILHEMAHKFTAMKYGFWAEFRKDNLMLLVAVAMAALVGV
                     VFAAPGATVIYGTYISREQNGKISAAGPAVNILLCIPFALILFVSGMFPVSFLSRDLL
                     ALVGMVGLQVNAMIAAFNMLPVSVLDGKKVLAWNPAVFAVLIIAAFGILIASFYPSFF
                     "
BASE COUNT          627 a          695 c          698 g          434 t
ORIGIN      
        1 ataacggtac cggatgtccc ttgcctcaag gatgattctg ctcattttgg tgactcctat
       61 atcaacgtga catgggttgt ggccgcaacg actcctgcag acatgaaaag aaacgagatg
      121 accgcaagga cggacggaag ggagaatggt ctcccttctc ccagcactgc aaatttcccc
      181 tcgtagcaac gggcctccat tgcccgggtc aggtcttccc ccgcctccca gctggcgata
      241 aagactgcac ccgcaagggt ggcaaacgag cgtatcgatt cgcggaatgt gcggtacccg
      301 aggcgcatca tctgtgcctg gtagaccatc accaggtggt ccatgatcag gaagatagta
      361 cggtagatga tcatggcaag gtcaagcact tcctcgggaa cccgtgcacg ccgcatcacc
      421 tggaagagat ccgtcatggg ggtggaaaac gcgataaaga ggagggcgga agtgccacca
      481 acgacgcgga agaagacaaa caccccctgg ttgatgctct cccgtgtgac agagagcgag
      541 agccatggaa agatctgcca gctccagaag atctctccgc ctccagcaag gagaatgatt
      601 gcgcccgcgc ccaggcctgc gaagaggagc ggcgcaacga aggcctttgc gtagatcgcg
      661 ggatcgatgc gggcgataaa aatgagagct cccgcaaata cgagggcaat gaagaggggg
      721 gccacgtaac tcgaagagag gaggcagagg aggattgccc cgagccccgc ggccagcttg
      781 gtataggtat tgacctccct cagcctgttc tgctgggcga tgtcctcaag gagctcttca
      841 tacatggagc actgcacata gatgttggtc ccttcaggtg cttctattct tctggcctct
      901 ccagaacccg aacacccatc ccacgatgat tccacctacc gctgcctgga gcgcaaagag
      961 accggactcg atctctccgc tcggcggctc ccactgcggg acaaggggct ggaaatcctc
     1021 ctccgagatg cctgcaagtt ccgcggccct tgatgacccc agggtgtcgg aaccggcata
     1081 ttcggcatcg gcattcaggg aacttgtata gaggaagacc ccgcagaagc agacgatagc
     1141 tgcaactgcg atgatctcga gacggtactt cgcccacatc aggcatcacc tgctgctgcc
     1201 ctgatctttt cctcggggaa caccttcagg cggatgagga tctcgggcct gagatggacg
     1261 atgtacttga agaccagggc gagcacgacc ccttccacga tcgcaagcgg cacctgggtg
     1321 atagcaaaga tacccatgaa caagacgaag gaccctgcaa acccacccac ttcggcagga
     1381 tatgccagtg cgagctccag tgacgtcacg atatacgtgc agaggtcggc aatggcacag
     1441 gcaaagaaca ctacaaaata catgtttact cctgtatctc gaagggcacg gtagatcgcc
     1501 catccggcga ggggaccgac gacccccatg gagatgatgt ttgcaccaag taccgagagg
     1561 cctccatggg ccaggaagag gctctggaag aggagcacta tcgcgcagat gaccgatgtg
     1621 acccacggcc caaagcagac cgaagagatg cctgttccgg tcggatgcga acagcttccc
     1681 gtgaccgaag ggagtttcaa ggacgaaagg atgaagatga agcctcccgt cacccccagc
     1741 aggggcaggg cttcgcggtc ctggccgagg atcctcttca actggaggac gccgaacgca
     1801 aggcacggga gggcgagcac ccaccagaac accgcccatt ggagagggag aaaaccttcc
     1861 atgatatgca tgatcagatc accttaaaca atttaatttg atttagaaca aattaatctg
     1921 attaatattt atgccttccc ttttcaccga ccggtgaaaa ggaaaaaagg ttcagaaaaa
     1981 ggagggataa aaagatgcga tgagaatgcc gaaggcggca atgatgagga ccgcgaatac
     2041 cgcggggttc cacgcgagca ctttctttcc gtcaagcacg ctcaccggga gcatgttgaa
     2101 cgcggcgatc atcgcattca cctgaagccc caccatccca acgagggcaa ggaggtccct
     2161 cgaaaggaag gaaacaggga acatcccgga tacgaagagg atgagcgcaa acgggatgca
     2221 gaggaggata ttgaccgcag gccctgccgc ggatatcttc ccgttctgct ccctgctgat
     2281 gtaggttccg tagatcactg tggccccggg tgctgcaaag acgactccga caagggctgc
     2341 catcgccacc gccacgagga gcatcaggtt atccttccga aactcggccc agaacccgta
     2401 cttcatagcc gtgaacttgt gcgccatctc gtggaggatg aacccgatcc ccac
//
LOCUS       DAOQVJ010000229         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52246_length_2454_cov_3.566903, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000229 DAOQVJ010000000
VERSION     DAOQVJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52246_length_2454_cov_3.566903"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1162
                     /locus_tag="PKL26_05550"
     CDS             131..1162
                     /locus_tag="PKL26_05550"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HNS83195.1"
                     /translation="MTANDRDFRDAADTTAKRIRSLVPGDLAEMFRQETKITPGEFHP
                     CPFVPPGEVCAVDGSNGLLLESGSMAIALFRAAQSTYHNRERHRRSLSPLRFGVIGPS
                     QENEDFYELYEECFGELPGTPLGNEDRSRVAGVLRDTLEYWITEQMAHSLSPGALLLR
                     DGPLRVSHASHDPVLSRIEDTCNARGISLVGVSKRTSATWGGGYPLVPTLAGLAEAFG
                     ISPPWWIRIDPNILDHAQYPQWQHGQMYVTCLHPHSKSPLKLELTAGIPEQRVREIMG
                     RLGACSMDGRIPGYPYPLLDAHRTVVLSEEIMEQARSDLIRRIVASGIKLRTYEILFG
                     DYHDEFARY"
     gene            1140..>2454
                     /locus_tag="PKL26_05555"
     CDS             1140..>2454
                     /locus_tag="PKL26_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNS83196.1"
                     /translation="MMNLRDIDMNDTSKYRLIGRSVLSYRFIAPYDTALYIGEIKKIS
                     DTTKDVTFFAKITDLSHDSNFADARWDTRVYAEEFYGLGEDVFLSVDAVPLGFVDNAT
                     GAFHKPRTIPSKFSTIGDPDAEDFRFLTNLMGEIEVGLMRSGQDVIRDVAVRIPSRIL
                     PQHMGVFATTGMGKSNFMRVFCASCMKERKFGLLIVDPHGEYVSGGRSSIGEPTSGLV
                     HYTEGKDGLSIFTIRHETDRKKYGLNRLSIEYDDFRIGDLAILYDLSAAQAEIVEAFA
                     GVSGSEVIGFFRAIDPEIFPIRDPVAAGVAEGSLAWKIRNSMGGPVRVIKSHIENIVN
                     GNRTFFKPKGSSLSEIISHLHENRVVLVDIPDMSERSELFVLSIITRTIMERHRSEAR
                     SFGIDERQESSHQVLITIEEAQRVLASGGASTQVFRECAMEGRKFG"
BASE COUNT          525 a          716 c          714 g          499 t
ORIGIN      
        1 tcaggacaca ggagtcccgt tttccccaga tcatcctgat atcccatata cccgacatcc
       61 agggagagtt tgcgaacacg ctgatggtgg agatgggaaa cgacctttcg agccgggtac
      121 aggaagtgac atgacggcga atgaccgtga cttccgggat gcggcagata ccactgcgaa
      181 gcgcatcagg agcctcgtgc ccggagatct tgccgaaatg ttcaggcagg agacaaaaat
      241 cacgccgggc gagttccacc catgcccctt tgttccacca ggggaggtct gcgcggtgga
      301 tgggagcaat gggctcctgc tcgaatcggg aagtatggct atcgcgctgt tccgtgccgc
      361 ccagagcact taccacaacc gcgagaggca ccggagatct ctctctcccc tgcggttcgg
      421 agtcattggt ccctctcagg agaacgagga cttttacgaa ctctacgagg aatgcttcgg
      481 cgaattgccg gggactcccc tcggaaacga ggatcggagc cgggttgcag gggtccttcg
      541 ggacaccctc gaatactgga tcacggaaca gatggcgcat tccctctcgc ccggtgccct
      601 tctcctccgc gacgggcccc ttcgtgtctc acatgcgagc cacgaccctg tcctctccag
      661 gatcgaggat acgtgcaacg cgagaggtat cagcctggtc ggggtgagca agcggacatc
      721 agccacctgg ggaggcggct acccgcttgt cccaaccctt gccggactcg cagaggcgtt
      781 tgggatatcc cccccctggt ggatccgtat cgaccctaat atccttgacc acgcccagta
      841 tccccagtgg cagcatgggc agatgtatgt cacctgtctg catccccact caaagtcacc
      901 attgaaactg gagctgacag ccggcatccc cgaacagcgg gtccgggaga tcatggggcg
      961 ccttggggcc tgttccatgg atggccgtat ccctggatac ccctatcccc tccttgatgc
     1021 ccatcggaca gtggtgctca gcgaggagat catggagcag gcaagatccg acctgattcg
     1081 acgtatcgtc gcctcgggga taaaactgcg gacatacgag atcctttttg gagattacca
     1141 tgatgaattt gcgcgatatt gacatgaatg acacgtccaa gtaccggttg atcgggcgat
     1201 cggtcctctc ctaccggttt attgcaccct acgacacggc gctttacatc ggggagatca
     1261 agaagatctc cgatactacg aaggatgtga ccttctttgc aaagatcacc gacctctccc
     1321 acgacagcaa cttcgctgat gcccgctggg acacccgcgt atatgccgag gagttctacg
     1381 gtctcggaga agacgtcttt ctctctgtcg atgctgtccc tctcgggttc gtggataacg
     1441 cgacaggggc attccacaag ccgcggacga ttccctcgaa attctccacg ataggggatc
     1501 ctgatgcgga ggacttccgt ttcctcacca acctgatggg agagatcgag gtcggcctga
     1561 tgaggagcgg acaggacgta atccgcgacg tcgccgtgcg tatcccaagc cgcatcctcc
     1621 cccagcacat gggtgtattt gcgacgaccg ggatgggcaa gagcaacttc atgcgggtct
     1681 tttgcgcctc ctgcatgaaa gagagaaaat tcggcctcct cattgttgat ccgcacggtg
     1741 aatacgtcag cggcgggagg tcatcgattg gcgagccgac ctccggcctt gtccactaca
     1801 cggaaggaaa agacgggctc tccatcttca ccatccgtca cgagaccgac aggaaaaagt
     1861 acgggctgaa ccggctctcg atcgaatacg acgacttccg gatcggcgat cttgctatcc
     1921 tttacgacct ctctgcagct caagccgaga tcgtcgaggc gttcgcgggt gtatccggga
     1981 gcgaggtcat cggttttttc agggcaatcg accccgagat ctttcccata agggacccgg
     2041 ttgcggccgg agttgcggag gggagccttg catggaagat ccggaattct atgggtgggc
     2101 ctgtccgggt gatcaagagc catatcgaga acatcgtgaa cggtaacagg actttcttca
     2161 agccgaaggg atcgtccctt tccgagatca tctcccacct ccatgagaac cgcgtggtgc
     2221 tggtagacat ccccgacatg agcgagagga gcgagctctt cgtgctctcg atcatcaccc
     2281 gcacgatcat ggagaggcac cgatccgagg cacgttcatt cgggatcgac gagcgccagg
     2341 agtcctctca ccaggtcctt atcacaatcg aggaggcgca gcgtgtgctt gcaagcggcg
     2401 gggcatccac gcaggtattc agggagtgtg ccatggaagg gaggaaattc gggg
//
LOCUS       DAOQVJ010000230         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52369_length_2450_cov_3.905637, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000230 DAOQVJ010000000
VERSION     DAOQVJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52369_length_2450_cov_3.905637"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(556..1104)
                     /locus_tag="PKL26_05560"
     CDS             complement(556..1104)
                     /locus_tag="PKL26_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83197.1"
                     /translation="MQKEPDTIYLGVERAPDFSLPFNQRMGIRDRGEPPVNGRCESRM
                     GEILLQFLLAHQVISHTDCGCQSDFFRQFLAPFHIAVRDVDIHILERTTLGGDEVAIG
                     SFYLCFEREGSSAGKNVAAQGLYTVRCNGCDTWVKFTSSTYQFRVHIRGPDPASVTIS
                     HNGQVIETGPLSSGPAPDPTSS"
     gene            1257..2204
                     /locus_tag="PKL26_05565"
     CDS             1257..2204
                     /locus_tag="PKL26_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNS83198.1"
                     /translation="MEYASTYHHEITGQDERRSWLDYIEKTWEFFLYSSLYLGIAAAG
                     MAYVTCLIEGAGFSLPAAAILGLVVFSVYNLNRKTDEAEDALNHACRHRITSTFSRPL
                     LAMAVVAYAAALCLAAPFGIAALGITLVPLIAGSLYSLPLLPPTTGYSRLKEVPVGKN
                     LVVSFSWGFSFSLLPVAIARIDPGPATLAAFFFIFSWTFIASVLPDTRDRAGDAATGV
                     VTIPVLLGVPWTRRLLSAFNFISGFLLLVPWTIIPSRIGAGLIAVSLMYSQACIIAIG
                     RTCHEGFLCDVVSDGQFIPIALLGFLVARGFPIFGSCVL"
BASE COUNT          515 a          701 c          620 g          614 t
ORIGIN      
        1 gaacattgcg atgccgggag atgatcgctc ctgctaccag gtgcctgaat tcatgcagtt
       61 caggagggaa tgagatgacc gcaacgtcgc cgatgatctg gaagccatcc gggatccttt
      121 tggcaacctc gtcaggaagc gttccccgca gatcatcctt caaagccatt gtatgatacc
      181 aggtgattgt cgcatgaatg aaaaaagcct cctcgctgtt ttatgtgggg agactgaatc
      241 gaggttggta tctgcatatc aattcagtca ctccaaccgc cgccgtgctt ccgcacagaa
      301 cttctcccag attactcagt aatggcggta aatctgaagg tgaccgcagg acgccccggg
      361 tcgagggcgt ataccccgcg gtatgttcgg gggcggcggc gtgtatcgca tatgggggtg
      421 cggggtcgga cagcccccac aaaatgtgtc cgattcacca atcctgccca caacagatag
      481 cgaagaaccg tataaaaact tccacgagaa ggagagacca ctcatgaagt acgactccct
      541 ccactcaagt tttttttagg aactggtggg atccggtgcc gggccggaag agagtggccc
      601 tgtctcaatg acctgtccgt tatgggatat ggtgactgat gcgggatcgg ggcccctgat
      661 atgcactctg aactggtacg tgctcgatgt aaactttacc caggtatcgc aaccattgca
      721 acggacggta tacagtccct gtgctgctac gttctttccc gcagaagaac cctctctctc
      781 aaaacagaga tagaaagaac caattgcaac ctcgtcccca ccgagagtgg tcctttcaag
      841 gatatgaata tccacatcgc gcacggcaat atggaatgga gcaaggaact gcctgaagaa
      901 atccgactgg cacccgcaat cggtgtgtga aataacctgg tgtgcaagga gaaactggag
      961 caggatctcc cccatccggg actcacaccg tccattcacc ggtggttccc ccctgtcccg
     1021 gattcccatt ctctggttaa aggggaggct gaagtcagga gcacgctcca cgcccaggta
     1081 gatagtgtct ggttctttct gcacgatcgt tgaagcatac tctctccagt gaaccctgac
     1141 tccagtgagt gtgttgaatg tgtggtgcca tatttatcac ggagtttcat attcatcgca
     1201 gcgatgaata ttcatcatca caatgaatgt gaatctcatc ggcaattctt catctcatgg
     1261 aatacgcaag tacataccac catgagatca cgggccagga tgaacgtcgc tcctggcttg
     1321 actatataga aaaaacatgg gaattcttcc tctacagctc gctctatctc ggcatcgccg
     1381 ccgcagggat ggcatacgtg acctgcctca tcgaaggtgc gggcttctcc ctgccggccg
     1441 ccgctatact tggtctcgtg gtcttttcgg tctataacct gaaccggaag accgacgagg
     1501 cagaggatgc cctcaaccat gcctgccgtc acaggatcac cagcacattc tcccgcccgc
     1561 ttcttgcaat ggcggttgtt gcgtacgcag ctgccctttg ccttgccgcg ccgttcggga
     1621 tcgcggctct cggaatcact cttgtccctc tcatagctgg atcgctgtac agcttgccgc
     1681 ttcttccacc aacgacaggg tatagccgtc tcaaggaggt tccggttggg aagaacctgg
     1741 tggtatcttt ctcctggggt ttctccttct cacttcttcc tgtcgccatc gcccgcatcg
     1801 accctggccc cgcaaccctt gctgcgttct tcttcatctt cagctggaca ttcattgcat
     1861 ctgtccttcc tgataccagg gaccgtgccg gtgatgcggc aaccggcgtg gtcactatcc
     1921 ccgttctcct cggcgtgccg tggaccagga ggctcctttc cgcgttcaat ttcatttcgg
     1981 gatttctcct gcttgttccc tggactatta tcccttcacg gattggagca ggactgattg
     2041 ccgtttccct tatgtattca caggcctgca tcattgcaat cggccgcacg tgccacgagg
     2101 gttttctctg cgatgtcgtg tcagatgggc agttcattcc tatcgcgctc ttagggtttc
     2161 tcgttgccag ggggttccct atctttggat catgtgtact ttgaatgtcc cgtgttggag
     2221 tgtccctgtc gggttccgat cgaatgaaat atttttcggt cagtgataca tgtatctgat
     2281 atggatatcc tggaggtcta ccatcagcat tccaggctga tccactccat atacaactcg
     2341 cgcctcaaga tccaggtgct gctggctctc ctcgacggca aggcgtcgct ctcgtcactc
     2401 cggagcatca ccggcagcac ttccccggcc ctcattccca agatacggaa
//
LOCUS       DAOQVJ010000231         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52567_length_2444_cov_4.799498, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000231 DAOQVJ010000000
VERSION     DAOQVJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52567_length_2444_cov_4.799498"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKL26_05570"
     CDS             <1..630
                     /locus_tag="PKL26_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83199.1"
                     /translation="FDNVAANHTISAVFLPDGSLTFYIQATAFTGGNIIPSGNVSVLG
                     GNDITFTIYPDSGHVVQNVMVDGDSKGGLLDWTFRDVAANHTISATFAPSGGGGGGGG
                     GGGGGGYSVSTTTQPTTVPTTPVAETEGYESGTSEETITPTPTATAEVTGVETIVTTV
                     PTTIPPAEPFWTRFPLYWLVPLIIVILILAAVAYYYWKKKQQQEVLFEE"
     gene            818..1786
                     /locus_tag="PKL26_05575"
     CDS             818..1786
                     /locus_tag="PKL26_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HNS83200.1"
                     /translation="MNDTTMIWIEKYRPQTLADIVGQDEIVERLQSYVKTGNLPHLLF
                     TGSAGVGKTTAAVALAREFFGESWNLNFRELNASDERGIDVVRNQIKQFARTSPLGGA
                     TFKILFLDEADALTPDAQAALRRTMESYATTCRFILSCNYSSKIIDPIQSRCAIYRFR
                     SLSGEAITKEILRIAENEKISITEPAIQAIVYIAQGDMRKAINALQGAAILAAEIDAG
                     MVYAITATARPDEIEDLLATSLSGDFEGAEAILHHLLQDRGIAPNELINQCYRTIVKR
                     DMDPELRVALIDQLGTTDFRLSEGAGTEIQMEAMIAQFVLQAKKHG"
     gene            1779..>2444
                     /locus_tag="PKL26_05580"
     CDS             1779..>2444
                     /locus_tag="PKL26_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS83201.1"
                     /translation="MAKRADSPAAEAASEIVDRDADWSRFLKSRYKKQLSELAREYPY
                     RRSLQIDYRELESYGRAGLRMADELLDNPGKVIEDVRNAIKNHQLVKGRDGKPTGEVN
                     VRFVNLPRKIGIRHIRSDHINRFISVEGILRKTTEVRPRIVQAVFRCPGGHVTIREQG
                     YGKFREPDGCATEGCTFKKLELIPRRSRFVDSQKLRIQESPEGLRGGEQPQTLDVDVT
                     DDLT"
BASE COUNT          556 a          751 c          701 g          436 t
ORIGIN      
        1 ttcgacaacg ttgccgcaaa ccataccatc tccgcagtct tcctgccgga cgggtccctg
       61 accttctaca tccaggcaac tgcctttacg ggggggaaca tcattccctc agggaacgtg
      121 agtgtccttg gaggaaacga catcacgttc accatatatc ctgactccgg ccatgtcgtt
      181 cagaacgtca tggtggacgg tgacagcaag ggcgggctcc ttgactggac attcagggac
      241 gttgcggcga accataccat cagtgcaacc ttcgcaccct ccggtggcgg cggcggaggc
      301 ggtggtggcg gtggcggtgg cggctactcc gtgagcacga caacccagcc caccacggtc
      361 cccactacgc cggtcgccga gaccgaggga tacgagagcg gtacaagtga agagacgatc
      421 acccccactc ctacagccac tgccgaggtg accggggttg agacgattgt caccacggta
      481 cctaccacga ttccccctgc cgaacctttc tggaccagat tcccgctcta ctggctcgtg
      541 ccactgatca ttgtcatcct catcctggcc gctgttgcat actactactg gaagaagaaa
      601 cagcagcagg aagttctgtt cgaagaataa tgacctgaat tctctttttt ttatcctctc
      661 atagagagcg tgccagggcg gtgcctggct ttcccagcgg gattgtttca ccaggcactt
      721 cgacatggca tccacccaat cacaacacat ttcttctcct gcccccgata tagtggaggt
      781 aagatttgcg gactttttac aggggtggag aagcgcgatg aacgatacca cgatgatatg
      841 gatagagaag taccgtccgc aaacccttgc ggatatcgtg ggccaggacg agatcgtcga
      901 gcgcctgcag tcctatgtga agaccgggaa cctgccgcac ctcctcttca ccggaagtgc
      961 gggggtgggc aagaccaccg cggcagtcgc actggcccgg gaattcttcg gggagtcctg
     1021 gaacctgaac ttcagggaac tgaacgcgtc agacgagcgg ggcatagacg tcgtacggaa
     1081 ccagatcaaa cagttcgccc ggacttctcc gcttggcggt gcaactttca agatactctt
     1141 cctggacgag gccgacgccc tcacccccga cgcccaggcg gcgcttcggc gaaccatgga
     1201 gagctacgcc accacctgca ggttcatcct ctcctgcaac tactcctcga agatcatcga
     1261 tcccatacaa agccggtgcg caatctaccg gttcaggagc ctctcgggtg aggccatcac
     1321 aaaggagata ctgcgaatcg cagagaacga gaagatcagc atcacggagc ccgcaatcca
     1381 ggcgatcgta tacatcgccc agggggacat gcggaaagcc atcaatgcac tccagggcgc
     1441 agccatcctt gcggccgaga tcgacgccgg gatggtctat gccatcaccg caacggcacg
     1501 ccccgacgag atcgaggatc tgctggccac gtcactctcc ggtgactttg aaggggcgga
     1561 ggcgatcctc caccaccttc tccaggaccg gggaattgcc ccgaacgaac tgatcaacca
     1621 gtgctacagg accatcgtca aaagggacat ggaccccgaa ctccgggtag ccctcatcga
     1681 ccagcttggg accacggatt tccgcctctc tgaaggagcg ggcacagaga tccagatgga
     1741 agccatgatc gcccagttcg tgctccaggc aaagaagcat ggctaaacga gcagattccc
     1801 cggccgccga ggccgcgtcc gagatcgtcg atcgcgatgc cgactggagc aggttcttaa
     1861 aaagccgcta taaaaagcaa ctctcggaac ttgcccggga gtatccttac cggcgctccc
     1921 tccagatcga ttaccgcgag ctggagagct atggccgtgc gggcctccgc atggccgatg
     1981 agctcctgga caaccccgga aaggtcatcg aggacgtgcg aaacgccata aagaaccacc
     2041 agctcgtgaa ggggagggac gggaagccta ccggcgaggt gaatgtccgg ttcgtgaacc
     2101 ttccccgcaa gatcgggatc cggcacatcc gttcggacca catcaaccgc ttcatcagcg
     2161 tggaggggat cctcaggaag accacagaag tgcggccccg catcgtgcag gcggtgttca
     2221 ggtgccctgg cgggcatgtc accatccggg agcagggata cggtaagttc agggaaccgg
     2281 acgggtgtgc aaccgaggga tgcacgttca agaagctcga gctcatcccg cggcgatccc
     2341 gcttcgtgga ttcgcagaaa ctccgcatcc aggaatcccc tgaagggctc aggggaggcg
     2401 agcagcccca gaccctggac gtggacgtca ctgacgatct gaca
//
LOCUS       DAOQVJ010000232         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52607_length_2443_cov_3.654523, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000232 DAOQVJ010000000
VERSION     DAOQVJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52607_length_2443_cov_3.654523"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKL26_05585"
     CDS             <1..765
                     /locus_tag="PKL26_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNS83202.1"
                     /translation="PARVYPGVSYRNLLVVPDAGGAKTVPPHDIVGQEVAPHLPSGRD
                     APLLLECMEASQRIFAGHPVNRDRVSRGLLPATQIWPWSGGRVPLLTPFSDKYGKRGG
                     IISAVDLLKGIGRCSGMEVIEVPGATGYLDTDYQAKARYALFALEHLDFVYIHVEAPD
                     EAGHLGSIKEKVTAIERLDEMIGSIMKEFHGVVAVLPDHPTPIRIRTHSGDPVPFAIF
                     GKARDSCTCFSEKEAAGGVCGLMEGPDFLSFLFCED"
     gene            840..2372
                     /locus_tag="PKL26_05590"
     CDS             840..2372
                     /locus_tag="PKL26_05590"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HNS83203.1"
                     /translation="MNEAWLFDELERVAGPLTPLQRILLGTDGSVTRILELATGAPVA
                     ITTLQQTVEAADLLVAQRLDVPPGQKVNHRIVELKNTRTHETLIYAESFTPLSRLSPS
                     FRDDLMRADIPIGKILEQHQLETRREIVKMSAGPRPAPVAESFGLSGNPRFLSRQYRI
                     IHHEHPLIHIEEVFPAGLFSGEIRVVIDAPSRLHLGLLDMNGSLGRIDGGIGLALDEP
                     RLVLSARKSEAFAVEGGDADARERVLAAAAAVSKSLNLPGAAEFVVQEDFPGHVGLGR
                     GTQLALSTACALCRLYGQEWNTRDLARMTGRGGTSGIGTASFATGGFLLDGGHSFGVV
                     MEKAAFLPSSASRGVRPPEVLLRREFPEAWKVLLVIPDVPPGASGGTEKDLFSRYCPV
                     PLEEVRELCHLVVASLLPGLAEEDLDLFGSAINRMQELGFKKVENTLQAPGVGDLVRA
                     MREAGAAGAGLSSFGPAVYAFGEGRMHDVESAALEILPSLGGGRVLLTQARNSGAVVS
                     MA"
BASE COUNT          422 a          765 c          795 g          461 t
ORIGIN      
        1 cctgcacggg tctacccggg cgtgagctat cgcaacctcc tggtcgtgcc ggatgcaggg
       61 ggagcaaaga ccgtccctcc ccacgacatc gtgggccagg aagtcgcgcc ccacctccct
      121 tcgggcaggg atgctcccct gctcctggag tgcatggagg cctcgcagag gatctttgcg
      181 ggacacccgg tgaacaggga ccgcgtctcc cgcggactcc tcccggccac ccagatatgg
      241 ccatggagcg ggggcagggt ccccctgctc accccttttt ccgacaagta cgggaagcgg
      301 gggggcatca tatccgcagt cgacctcctc aaggggatag ggaggtgctc gggaatggag
      361 gtcatcgagg tccccggcgc gacggggtac cttgacactg actaccaggc caaggcccgg
      421 tatgcactct ttgcgctgga acacctggac ttcgtctata tccacgtcga ggcacccgac
      481 gaggcagggc acctgggaag catcaaagag aaggtgactg ccatcgagcg gctggatgag
      541 atgatcggtt ccatcatgaa ggagttccat ggtgtcgtcg ccgtgctccc cgaccacccg
      601 acgccgatac ggatccggac ccattcaggc gaccccgtgc cctttgcgat ctttgggaag
      661 gccagggaca gctgcacctg tttctccgaa aaggaggccg cgggaggagt gtgcggactg
      721 atggagggtc ctgacttcct ctccttcctc ttctgcgagg actgacgctt cccgaacctc
      781 ccgcggtcga agatccaggg ggaaagcttg tatctcccca ccaccatcac tggtgtggca
      841 tgaacgaggc ttggctgttc gacgaactcg aacgggttgc cggaccgctc accccgctgc
      901 agaggatact gctcgggact gacgggtctg tgacaaggat cctggaactg gcaaccgggg
      961 caccggttgc catcaccacc ctgcagcaga cggtggaggc agcggatttg ctggttgcgc
     1021 agagactgga tgtcccgcca gggcagaaag tcaaccaccg tatcgtggag ctgaaaaaca
     1081 cccggacaca tgagacgctc atctacgcgg agtcctttac cccgctctcc aggctctccc
     1141 cgtctttccg ggacgacctg atgcgcgccg acatcccgat cgggaagatc ctcgagcagc
     1201 atcagctgga gacgaggcgg gagatcgtga agatgtccgc cgggccccgg cctgcacccg
     1261 ttgccgagtc tttcggcctc tcgggaaacc cgcggttcct ctccaggcag taccggatca
     1321 tccaccatga gcatcccctt attcacatcg aggaggtctt ccctgccggg ctcttctcgg
     1381 gagagatacg ggtagtgatc gatgcaccct cgaggctgca cctgggcctc cttgacatga
     1441 acggctcgct cgggaggatc gacgggggaa tcgggcttgc gctcgacgag ccacgcctcg
     1501 tcctctctgc acggaagagc gaggcgttcg ccgtcgaagg tggcgatgca gacgcccggg
     1561 agagggtact tgccgcggcg gcagccgtct caaaatccct gaatcttcca ggtgccgcgg
     1621 agttcgtcgt tcaggaggac ttccctggcc acgtgggact cggccgcggg acgcagcttg
     1681 ccctctcgac cgcatgcgct ctctgccgcc tttatggcca ggagtggaat acccgtgacc
     1741 ttgcgcgcat gaccggtcgc gggggcactt cagggattgg gactgcatcg tttgcgacag
     1801 gaggatttct ccttgacggg gggcattcgt ttggcgtggt catggagaag gccgccttcc
     1861 tcccgtcgtc ggcctcccgg ggggtccgcc cgccagaggt gctcctgcgg cgagaatttc
     1921 cggaggcctg gaaagtcctg ctcgtcatcc cggatgtccc gcccggggca agcggaggga
     1981 ccgagaagga tctcttctcc cggtattgtc ccgtgcctct cgaggaggtc cgcgaactct
     2041 gccaccttgt ggtggcctcg ctcctccccg gccttgccga ggaggacctg gacctctttg
     2101 gttctgccat caaccggatg caggaactcg ggttcaagaa ggtcgagaat acccttcagg
     2161 ctccaggggt cggcgacctc gtgagggcga tgcgggaggc gggtgccgcg ggtgcggggc
     2221 tctcttcgtt cgggcctgcg gtgtatgcat tcggggaggg gcgcatgcat gacgtggaga
     2281 gtgcggcatt ggagatcctc ccctcactcg ggggaggtcg cgtgctcctc acgcaggcga
     2341 ggaattccgg cgctgtggtc agcatggcct gacccccgtt catggcctgg ccggaggcac
     2401 tttcattaga aatactgctc cagtatggta ctatgagcgg gcc
//
LOCUS       DAOQVJ010000233         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_52793_length_2438_cov_3.604280, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000233 DAOQVJ010000000
VERSION     DAOQVJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52793_length_2438_cov_3.604280"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1910)
                     /locus_tag="PKL26_05595"
     CDS             complement(78..1910)
                     /locus_tag="PKL26_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNS83204.1"
                     /translation="MKRRQPKFLPMSKGEGKGLRIDQFDVILVSGDAYVDHPSFSTAI
                     IGRVLWDAGFTVGVIAQPDWKGNDDFLRLGDPRLFFAICPGNCDSLVNNYTAAKKRRS
                     SDVYSPGGIPRRPDRAGIVYTDRVHALFPGIPIVLGGIEASLRRFAHYDYWSDSVRHS
                     ILADAPADLLVYGMGEQQVREIALRLDKGEPPVSLTDIRGTVYRVPPAQYDGTCGFPA
                     LEIPPFSEVAADKVAYARAFATHYREQNPLRGRAVVQRHPKTIIIQNPPALPLSTQDL
                     DAIYELPYSREVHPSYTLPVPALEPVRFSLTSHRGCFGGCSFCALTHHQGRIIQSRSI
                     DSLVKEAIRLTRMRGFSGIISDVGGPTANMYGMECARWQTEGPCPDRACSVGCPNLLA
                     SHRRQMALLRRLREVPGVRRVFIGSGMRYDLIVADPEDPLPEMCAHHISGHLKVAPEH
                     TSPAVTALMGKPGKEVFERFLASFSKCQEGKEKKQYILPYFMSSHPGCTVGDMVDLAV
                     FIRDHDLYTEQVQDFTPTPMTVSTCMYYTGFNPLTLDPVHVPKGHEKQVQRALLRYRD
                     RHVRGLVEEGLCFAGRKDLLQKGRGGLLSSREKKGKSGMRQEEC"
     gene            complement(1907..2359)
                     /locus_tag="PKL26_05600"
     CDS             complement(1907..2359)
                     /locus_tag="PKL26_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNS83205.1"
                     /translation="MDCTEGRIGRVFVLRIDHGEDILETLLQCIREKEIFCGTVQVLG
                     ALGKGQMVTGSEEPVLPPLPHWEKVDGGWDIFGVGTISWGEDGPHLHLHSAVGRGTRS
                     LAGCLREQARVYIVVEAVILEINGIHTVRTHDETTGLTLPRFLPGNPP"
BASE COUNT          475 a          753 c          711 g          499 t
ORIGIN      
        1 aacgcgtacc gcatattcga cgagaagatg tcccggtgcc tcccctgcga gaacaggggg
       61 atcgatatcc caggacgcta acactcctcc tgcctcatcc ctgactttcc ctttttttcg
      121 cgcgaagaaa ggaggccccc cctccctttc tgcaggagat cttttctccc ggcaaaacaa
      181 agcccctctt ctaccaggcc cctgacatgc ctgtcccggt accggagaag ggcccgctgg
      241 acctgtttct catgtccttt cggcacatgg acaggatcga gggtgagggg attgaacccg
      301 gtatagtaca tgcaggtcga caccgtcatc ggcgtgggcg tgaaatcctg cacctgctcg
      361 gtgtagaggt cgtggtcgcg gatgaatact gcgaggtcga ccatgtcccc gacagtacac
      421 ccgggatggc tggacatgaa gtaagggaga atgtactgct ttttctcctt tccttcctgg
      481 catttgctga atgatgcaag gaaccgctcg aagacttcct ttcccggctt tcccatcaac
      541 gcggtgactg caggggaggt gtgctcgggg gcgaccttca ggtgtcccga tatgtggtgc
      601 gcgcacatct cgggaagggg atcttcgggg tctgccacga tgaggtcgta ccgcatgccg
      661 gacccgatga agacccttct cacacccggc acttctctta agcgcctgag aagtgccatc
      721 tgcctccggt gagaggccag gaggttcggg cacccgacgc tgcaggccct gtcagggcag
      781 ggtccttccg tctgccagcg ggcgcactcc atcccgtaca tgttcgcggt cggcccgccg
      841 acatcgctga taatccctga aaaaccccgc atccttgtca ggcggattgc ctcttttacg
      901 agcgaatcga tgctcctgct ctggattatg cgcccctggt gatgggtgag ggcgcaaaaa
      961 gaacagcctc caaagcaccc gcgatggctg gtgagcgaga acctgaccgg ttccagcgcc
     1021 gggacaggga gcgtatacga cgggtggacc tcccgggaat atggcagttc atagatggca
     1081 tccagatcct gcgtagagag agggagcgca ggcgggttct ggatgatgat ggtcttcggg
     1141 tgcctctgca cgaccgccct ccccctgaga gggttctgct cgcggtaatg cgtggcaaat
     1201 gcccgggcat aggcgacttt gtcggccgcc acttcggaaa atggaggtat ctcgagagcg
     1261 gggaatccgc atgtgccatc gtactgcgcg ggcgggaccc ggtagactgt tccacggata
     1321 tcggtcaggg atacgggggg ttctccctta tcgaggcgaa gggcaatctc cctgacctgc
     1381 tgctctccca ttccgtacac caggaggtct gcgggtgcat ccgcgaggat ggagtgacgg
     1441 acagagtcgg accagtaatc gtagtgggca aatctccgca gggacgcttc gatcccgccc
     1501 agcacgatag gaattccggg gaatagtgca tggaccctgt ccgtatagac gatccctgcc
     1561 ctatccggcc tccggggtat gcccccgggg gagtagacgt cgcttgagcg ccttttcttt
     1621 gcagccgtgt aattgttcac gagagagtca cagttgccgg ggcagatagc aaagaatagg
     1681 cgggggtccc cgagccgcag gaaatcatcg ttgcccttcc agtccggctg ggcgatcacc
     1741 cccacagtga acccggcgtc ccagagcacc ctgccgatga tcgcggttga gaatgaggga
     1801 tggtctacgt aggcgtcacc actgacaagg atcacgtcga actggtcgat tcggaggccc
     1861 tttccctccc ccttgctcat cggcaggaac ttcggctgtc tcctcttcat gggggattcc
     1921 ccgggagaaa ccggggaagg gtgagcccgg ttgtttcgtc gtgagtccgg accgtatgaa
     1981 tgccattgat ctcaagaatg accgcctcca ccacgatgta gactcgcgcc tgttcgcgga
     2041 ggcaccctgc cagtgaacgc gttcctcgtc ccaccgctga gtgaaggtgg aggtgcgggc
     2101 cgtcctctcc ccaggagata gtgcctaccc cgaagatatc ccacccacca tcaaccttct
     2161 cccagtgcgg gaggggaggg aggaccggtt cttctgaccc cgtcaccatc tgccccttcc
     2221 cgagtgcccc gaggacctgg acagtcccac agaaaatctc cttctcccgg atgcattgca
     2281 gaagtgtctc caggatgtct tctccgtgat cgatacggag tacaaagacc cggccaatgc
     2341 gtccttcagt gcagtccatg aagacctccc cttaacaaag ctccatcctt catcggccac
     2401 ccactccccc acctccgaat cctccgcccc ctccaaat
//
LOCUS       DAOQVJ010000234         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53193_length_2426_cov_4.143399, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000234 DAOQVJ010000000
VERSION     DAOQVJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53193_length_2426_cov_4.143399"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKL26_05605"
     CDS             complement(<1..290)
                     /locus_tag="PKL26_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83206.1"
                     /translation="MTEWNTETLAVLLVLAMIVLFPVGWVIIAFPESPYRPLSGEPVK
                     EAAESAGIRVVNVTDTTWHLPGATGGNTYVLEDGKGNRMVIRTQAFDSPDTR"
     gene            complement(319..1881)
                     /locus_tag="PKL26_05610"
                     /pseudo
     CDS             complement(319..1881)
                     /locus_tag="PKL26_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(1770..>2426)
                     /locus_tag="PKL26_05615"
     CDS             complement(1770..>2426)
                     /locus_tag="PKL26_05615"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991156.1"
                     /note="biodegradative; catalyzes the formation of agmatine
                     from arginine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNS83207.1"
                     /translation="PGVNEDGSLGSWGIPAAVVTKFLDTRGIVNEKSGDYVILFLFSM
                     GNTKGKWGTLITELFEFKRHYDEKTPIEEIFPELVNEYPERYSGMTLRSLAGEMHSFK
                     RERKMCELLEEAYSTLPEPAVAYAEAYRRIVRGEVEKVPVGKAGGRVVATGIYPYPPG
                     IPILAPGERAGPGEGPILQYLSCLQDFDARFPGFEHDIHGVENVRGEYMMYCIREESA
                     "
BASE COUNT          532 a          723 c          719 g          452 t
ORIGIN      
        1 ttcctcgtat ctggtgagtc aaacgcctgg gtgcggatca ccatcctgtt gccctttcca
       61 tcctccagga cgtaggtgtt cccacccgtt gctcccggga gatgccacgt ggtatcagtg
      121 acattcacga ccctgatacc tgctgactct gcggcctcct tcacgggttc gccggacagg
      181 ggacggtacg gagattcggg gaatgcaata atgacccagc ccaccgggaa gaggactatc
      241 atcgccagca cgagcagcac tgcaagggtt tcggtgttcc attctgtcat attctgcctc
      301 cattaggagc gaatggactc atcgagacac ctccctggtg gtcccggcct tcctccagcc
      361 aggcttcttc agttttaaaa agacgagcgg cggtactgcg aggaggaagg tgccgatcag
      421 tatccctccc acgtagaata cagccctcgt tgcggagaag tctcccgtag gcatcagccc
      481 gacaaaaaaa gagaagatta ctcctgctgc acccatcccc ccgatgagcc acgcgcccgc
      541 ggtcccccct gggattcgga acgggcgggg tgcatcgggg cgtgtgtacc ggaggcggat
      601 gagtgcggca aagacgagga agtacacgat gcaaagcagt tcgacggtta tcgcagagag
      661 tatccagtac gcctggttta ccgaaggcag gaagacatag agcagggaga tgagagtccc
      721 gatcatggcc tggacaatga gcactgcgac aggcgaaccg tagcggttgg tgcggttgaa
      781 gattggcggg aggttccctt cctctgcaac caccccgagg ccctttgcgg ggccgatcag
      841 ccaggcggca agggagacca ctcccccgat tgcaatcagg agggccatcg gtgcaacggc
      901 tccgggaatg cgggcggcat tgaagaagta ctggatcgcc tgcatcacgc ccgatgcaag
      961 gttcagttca tctgccggga tgacgaaggc gatcgcaagc gtggcaagga ccgtgcaccc
     1021 gaagatgatc agcgcggaga gcgccattgc tttcgggtaa tcggcaccag ggttccgcgt
     1081 ctccagggca tggaacccgg ccatctccat tccggcaaag aggaggatga cggtcgcaaa
     1141 gaacgggagg gtggagaggc tcacttcggg aattacctgg tcaggggaaa aagggggcag
     1201 aacgatcgtt gcaccggatg cgaaccacca gacgccgagc ataatgatca ggacagacgg
     1261 gatgatgctc ccgagaatca ccccggcatt ctggaacttt gtggatatat cctcaccaaa
     1321 gcaggcgatc gccgtcgtgc cccagaacgc gatcatcatc accacgaaaa gttacagcgg
     1381 gctttcgcca agggagggtg agagcgcaaa cgcgagggtc gaggcgatga atgcgagcac
     1441 cgtcggaaac cagacgaggt tgttcgacca ctcgcagaat atggcgacaa aaccgccttt
     1501 ctcgccgaat gcctccctgc accaggcata gacaccacca ccctggtccg agagcatcgt
     1561 tgcaagctcc gcccccgcga gagagagggg gatgaggaac atgactgtcc ctagaaggta
     1621 ccacccgatg caggaccagc cgaactctgc catggaagga aaattcctga tgctcagcac
     1681 cgccgcgaca ttgatcattg cgagggcaaa gatgccaagt acctttgtgg gggccggccc
     1741 ggccttcggc ggggatgtgt ctttcccgct cacgcggatt cctccctgat gcagtacatc
     1801 atgtattctc ccctcacatt ctcgacgccg tggatatcgt gctcgaatcc cgggaaccgt
     1861 gcatcgaagt cctggaggca tgagagatac tggaggatag gcccttctcc cggccctgcc
     1921 cgttcaccgg gagcgaggat gggaatcccc ggcgggtacg gatagatgcc ggtggcgacg
     1981 acccggcccc ctgccttccc cacgggaacc ttttcgacct ccccccgcac gatccgccgg
     2041 tatgcttccg cgtacgcaac ggcaggctcc ggcagggttg aataggcctc ctcgagcagt
     2101 tcgcacatct tccgttccct cttgaacgaa tgcatctcgc ccgcgaggct cctcaaagtc
     2161 atccccgaat accgctccgg gtattcgttc acgagttctg gaaagatctc ttcgatcggg
     2221 gtcttttcgt cgtaatggcg cttgaactcg aagagctcgg tgatgagtgt cccccacttg
     2281 cccttggtgt ttcccatcga gaagaggaag aggatgacat agtcgccgga cttctcgttc
     2341 acgatcccgc gcgtgtcaag gaactttgtg accaccgccg caggaattcc ccacgagcca
     2401 agggagccgt cctcattgac acccgg
//
LOCUS       DAOQVJ010000235         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53423_length_2420_cov_3.216490, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000235 DAOQVJ010000000
VERSION     DAOQVJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53423_length_2420_cov_3.216490"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKL26_05620"
     CDS             <1..656
                     /locus_tag="PKL26_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNS83208.1"
                     /translation="FRLGRGLLPFEDALVDRLLTLNPDLVVVDTSEGVSLIRVQGQDH
                     GAMQGGDPHVWLSPSNLRIMAGHMAEGFSIIDPGRAADYAAGKEAYIRRVDATDTNIR
                     DMLSGMEGKPFLVFHEAWGYFAREYGLVQVAVGAEGKEPGARELSSLITFARENGIRV
                     VFAEPQYDTRGSEVIAREIQGTVVLIDPLAPDTLSNLERVASAISLIYGEGNGGAVP"
     gene            653..1423
                     /locus_tag="PKL26_05625"
     CDS             653..1423
                     /locus_tag="PKL26_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNS83209.1"
                     /translation="MIPAIEVRDVSFGQEGSPILDRVSLSVMERDMYALIGPNGGGKT
                     TLLKIILGLLPARSGTVRIYGKSPGEGRQIVGYVPQFRTFDFRYPITVKEMVLSGRLS
                     HTRGMLRRYREEDHAAVRKVLERLDLSGLAGRTLSHLSGGEQQRAILARALAGNPRVL
                     ILDEPTVYVDTPTAFQFFDILDELKRSMTILLVSHDIGVISKHVTKVACLNHTLHTHN
                     TTEITEDMIAATYHCPVDLIAHGLPHRVFDDHGGEEGQ"
     gene            1420..2232
                     /locus_tag="PKL26_05630"
     CDS             1420..2232
                     /locus_tag="PKL26_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNS83210.1"
                     /translation="MIDLGILEYGFFQNALLAGLFASISCGIIGSYVVVRRMVSISGG
                     ISHAAFGGVGLGYLFGLDPLAGAFGFTTVIALGIGILKEKAGQNLDTLVAAVWASGMA
                     LGIICISLAPGYAPDLFSYLFGNILLVPGQDILLMAVLCAIILAVVAILYTQLQAVTF
                     DEEYARVMNLPVTRLSLLLLVLVAVTVVMLIRVVGIILVIALLTLPAATARGFTKRLH
                     TMMAASVCLGLLFSIGGLLLSYLLDIPSGATIILLATAGYVISLPITRAARG"
BASE COUNT          437 a          714 c          752 g          517 t
ORIGIN      
        1 acttccggct tggccggggg ttgctgcctt tcgaggatgc gctggtcgac cgcctcttga
       61 ccctgaaccc ggatctcgtg gtcgtcgata cctcggaagg agtctccctc atcagggtgc
      121 aaggccagga ccacggcgcc atgcagggag gcgacccgca tgtctggctt tcgccttcga
      181 acctgcgcat catggcaggg cacatggcgg agggattctc gataattgat cccggccgtg
      241 cggccgatta tgcagcagga aaggaggcgt acatccgcag agtcgatgcg acggacacga
      301 acatcaggga catgctctcc ggcatggaag gaaaaccgtt cctggtattc cacgaggcgt
      361 gggggtactt tgcccgcgag tacgggctcg tccaggtggc agtgggcgca gagggcaagg
      421 aacccggtgc acgggagctt tcgtcgttga ttacctttgc gcgtgagaac ggaatacggg
      481 tggtatttgc cgaaccgcaa tatgacacgc gggggagcga ggtgatagcc cgggagatcc
      541 agggcacggt ggtgctgatc gacccactcg cccctgacac cctctcgaac ctcgagcggg
      601 tcgcttctgc gatctctttg atctatggag aggggaacgg gggtgccgtt ccgtgatccc
      661 ggcaatagag gtaagagacg tttcgttcgg gcaggaaggg agtcccatcc tggaccgcgt
      721 ctcgctctcc gtcatggagc gggacatgta tgcccttatc gggccaaacg gcggggggaa
      781 gaccaccctc ctcaagatca tccttggtct cctccccgca cggtccggaa cggtcagaat
      841 ctatgggaaa tccccgggag aggggaggca gatagtgggg tacgtgcccc agttccggac
      901 gttcgatttt cgctatccca tcaccgtgaa agagatggtc ctctcgggcc ggctctccca
      961 tacgagggga atgctcaggc ggtaccgcga ggaggaccac gcggctgttc gcaaggtcct
     1021 cgaaaggctt gacctttcgg ggctggcggg aaggacgctc tctcacctct ccggggggga
     1081 gcagcagcgg gcgatccttg cacgggccct ggccgggaat ccccgggtcc tcatcctcga
     1141 cgagccgaca gtatatgtcg atacccccac cgcgttccag ttcttcgata tcctggatga
     1201 actgaagagg tccatgacaa tcctcctggt gagccatgat atcggggtga tctcaaagca
     1261 cgtcacgaag gttgcctgcc tcaaccacac gctccatacg cacaatacca ccgagatcac
     1321 ggaggacatg atcgctgcga cctatcactg ccctgtagac ctcatcgcac acggactgcc
     1381 gcacagggtc ttcgatgatc acgggggtga ggaggggcaa tgatagacct tgggatcctg
     1441 gagtacgggt tcttccagaa cgccctcctt gcaggtctct ttgccagcat ctcctgcgga
     1501 atcatcggaa gctacgtggt ggtcaggcgc atggtctcga tctccggggg aatctcccac
     1561 gcggcgttcg ggggggtcgg actcggctat ctcttcggac tggaccccct cgcgggagcg
     1621 tttgggttca ccacggtaat cgcactcggg ataggtatcc tgaaggagaa ggccgggcag
     1681 aacctcgaca cccttgtggc ggcggtctgg gcatcgggaa tggcactcgg gatcatctgt
     1741 atcagccttg cgcccggtta tgcccctgac ctcttcagct atctctttgg caatatcctg
     1801 ctggtgccgg gacaagacat cctgttgatg gctgtgctct gcgccattat cctggccgtg
     1861 gtagcgatac tctacacgca gttgcaggcg gtcacgttcg acgaggagta tgcccgcgtg
     1921 atgaacctcc cggtgacccg gctctccctc ctcctgctgg tgcttgttgc agtcaccgtg
     1981 gtcatgctga taagggtggt gggaattatc cttgtcatcg cactcctcac cctccccgcg
     2041 gcaacggcac gcgggttcac aaagaggctc cataccatga tggcagcctc ggtctgtttg
     2101 ggccttttat tttccatcgg ggggcttttg ctgtcttacc tgctcgatat cccgtcaggc
     2161 gcaacgatca tccttctcgc aacggcagga tacgtgatct cactccccat tactcgggct
     2221 gcgaggggat gaatggttgg agaggggagg ttgcccgggc ttcctttcca cagcctgtac
     2281 gaaggaccgg atgttcgcgc gggttgcatc ggaggcttcg gcgtcagcgg cccggccttc
     2341 ctgctttttt cctgccctgc cctgcgtcgc cctccctgga atatctctcc cttccgcttg
     2401 acatcgaatg gttgaggagg
//
LOCUS       DAOQVJ010000236         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53462_length_2419_cov_3.611675, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000236 DAOQVJ010000000
VERSION     DAOQVJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53462_length_2419_cov_3.611675"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKL26_05635"
     CDS             complement(<1..732)
                     /locus_tag="PKL26_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS83211.1"
                     /translation="MNRFPLTVIVVFAVLCPIVLADAALPGDLDDNGVIAREELASSI
                     LGHLSQGTSISDTPISDLQDSAWVFVHWGGTARTVTDSSGKSLTLTRPLRRIIVMNSE
                     TLETMRSLGVTKERIVAVDKYIAQKPEFFPEYAGYPSVGSIWAPDYEKMLSLRPDALF
                     LYASVSKAECDEIERRVTASRPDLPVFRIDCYRPETYLEDAARVAEIFGKEEEGARLA
                     SFYTAALARVGSVAASSAPPEVYFET"
     gene            complement(729..1253)
                     /locus_tag="PKL26_05640"
     CDS             complement(729..1253)
                     /locus_tag="PKL26_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83212.1"
                     /translation="MKRGRLVTGIIPMIICAMLVQAAAAGIVERSIEPSEHSTYRVVL
                     TLPGETVAGIIEVLPSGMEVREVSLPPDQYRVDGATLFLAVIGEREITYFVHGQEDPA
                     GAITGTWTDYLSGNEGTVGGESDGTQGQGVPPAVPVTTGTAKAGASPLLLLGGAGLAF
                     IGVARCRARWRDEE"
     gene            1507..>2419
                     /locus_tag="PKL26_05645"
     CDS             1507..>2419
                     /locus_tag="PKL26_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS83213.1"
                     /translation="MIPIETSALTKYYGSLCAVDHLDLSIGNEIFGLLGPNGSGKTTT
                     VLMLTTLLRPTAGSARICGHDIESDAGEVRRLISYVPQDMAVDIKLTGRENVTLFAKL
                     YGISHRDETIDRVLGMMALSERADDLVRTYSGGMRRRLELAQALVHEPKVLFLDEPTI
                     GLDVAARKKIWEHIRTLKSRGMTIFVTTHYMDEADHFCDRVAILDHGRVAAVDTPARL
                     KGMLGKDLISARVSGGFSGDLPAGIERLGEKENGEYLFSSDHASEKVPVLFRWLEEHG
                     AVIHSLSVREPTLDDVFLALVGSRDDVT"
BASE COUNT          521 a          730 c          707 g          461 t
ORIGIN      
        1 ggtctcgaag tagacctcgg ggggtgcgga ggatgcggcg accgatccaa ccctcgcaag
       61 cgctgcggta tagaacgagg cgaggcgggc gccttcctcc tcctttccaa atatctccgc
      121 gacgcgggcc gcatcctcga gatacgtctc aggccggtag cagtcgatcc ggaagaccgg
      181 gaggtcgggc cttgatgccg tgaccctccg ctcgatctcg tcgcactctg ccttgctcac
      241 cgatgcatac aggaaaaggg catcaggacg aagcgagagc atcttttcgt agtccggggc
      301 ccagattgat ccgactgagg gatacccggc gtattccggg aaaaattccg gtttctgcgc
      361 gatgtacttg tccacggcca cgatcctctc ttttgtgacc ccgagggacc gcatcgtctc
      421 cagggtctca ctgttcatca cgataattcg gcgaagcggg cgcgtgaggg tgaggctctt
      481 tcctgaagag tcggtcaccg tcctggcagt cccgccccag tggacaaata cccatgcaga
      541 gtcctggagg tcggaaattg gggtatccga aatactggtg ccctgtgaga ggtggccgag
      601 gatcgaggag gcgagctcct ccctcgcgat gaccccgtta tcgtcaaggt cgccgggaag
      661 cgcggcatcg gccagaacaa tgggacagag gaccgcgaaa accacgatca ctgtcaacgg
      721 gaacctgttc attcctcgtc cctccaacga gcccggcatc tcgccacacc tatgaaggca
      781 agcccggccc ctcccaggag gagaaggggc gaagcccctg ccttcgcggt ccccgtggtc
      841 accggaactg ccgggggaac gccctggccc tgcgtgccgt ccgattcccc tccgactgtc
      901 ccctcgttgc cggaaagata gtctgtccac gtccccgtga tcgctcccgc tgggtcctct
      961 tgtccatgca cgaagtaggt gatctcgcgt tcaccgatca ccgcaagaaa gagtgtcgcc
     1021 ccatcgaccc tgtactggtc agggggcagg gagacctccc gcacctccat tccggagggg
     1081 agaacctcaa tgatcccggc gacagtctcc ccggggagag tcagcacgac gcggtaggta
     1141 gaatgctctg aaggctcgat ggaccgctct acaatgccgg ctgctgccgc ctggaccagc
     1201 atggcgcaga tgatcatggg aattatcccg gtgacaaggc gccctctctt catatggata
     1261 gttctttgac tgtgttacat taaataataa tggttataat ttcgtctgta ccaggaagag
     1321 aaaatagcaa tatatcacaa atttcgaaat atagtagcac taattgacaa attattttac
     1381 taacttcaac aatcaactcc attctctcct cctggaatga cacaatttac gacgggtcgg
     1441 aagtgctatc agcccaggaa cggaagagat tattctgaga ctctcccgca agacacgagc
     1501 gacgaaatga tcccgatcga gacatccgcc ctgacaaaat actacggatc actctgcgca
     1561 gtggaccacc tggacctctc catcggaaac gagatcttcg gcctgctcgg cccaaacggc
     1621 tccgggaaga ccacgacagt gctgatgctc accacgctgc tccggccgac cgcggggtct
     1681 gcgcgtatct gcggccacga tatcgagagc gatgcagggg aagtacgaag gctcatcagt
     1741 tacgtgcccc aggacatggc cgtcgacatc aagctgaccg ggagggagaa cgtgacactc
     1801 tttgcaaagc tctacgggat atcccaccgg gacgagacca ttgaccgcgt gctcgggatg
     1861 atggccctct cggaacgagc cgacgacctg gtccggacct actcgggagg gatgcggcgg
     1921 aggctcgaac ttgcccaggc actcgtccac gagccgaaag tcctcttcct tgacgagccc
     1981 accatcgggc tcgatgtcgc ggcaaggaag aagatctggg agcacatccg gaccctgaaa
     2041 tcccggggga tgacgatctt tgtcaccacc cactacatgg acgaggccga ccacttttgt
     2101 gaccgtgtcg cgatcctcga ccacggacgg gtggctgcag tcgacactcc cgcacgcctg
     2161 aaggggatgc ttggaaaaga cctcatcagt gcgagggtga gcggggggtt ctcgggagat
     2221 cttcccgcgg ggatcgagcg cctgggcgaa aaggagaacg gggagtatct gttctcatct
     2281 gaccatgcat ccgagaaggt gccggtgctc ttccgctggc tcgaggagca cggggcagtc
     2341 atccactccc tctcggtacg tgagccgacc ctcgacgacg tcttcctcgc gcttgtcggg
     2401 tcaagggacg acgtgacgg
//
LOCUS       DAOQVJ010000237         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53752_length_2411_cov_4.798387, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000237 DAOQVJ010000000
VERSION     DAOQVJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53752_length_2411_cov_4.798387"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKL26_05650"
     CDS             complement(<1..622)
                     /locus_tag="PKL26_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HNS83214.1"
                     /translation="MIKRFSLALIALVALAVLMLPASAATIVSVPAGGDVFIGEEGLD
                     ISAGLGAATQIAYWASGTSTGDQPTNIVTVGTPTSYYVSPQIFVGKVGTWYQWTGVRG
                     AAVFNVVDPALTVKVWDNTAGKDVTGKSVPPGNYLNFRVETNAYSVTARADAAGKGFV
                     NIRVKTADGATYTALFQTTTTSRPLTGLAPNSDLWYWAPQVTNQGWN"
     gene            1084..1797
                     /locus_tag="PKL26_05655"
     CDS             1084..1797
                     /locus_tag="PKL26_05655"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNS83215.1"
                     /translation="MRIIKAPTLASAHEQVVRMILEKGWTLDTEDREATVEFEEVAMR
                     VEQPGNPPMVSPCSRFQQRFMEQYARDLIEGSDSVFEYDYHDRLFNWGGGLMRGGGNE
                     VHVDQVAYIVHKLREAPASRRAIAVTWNPAIDENLDDCPCLQLVQCVSRDGLLHMKVV
                     FRSNDMLTAAGANMFALVSLQQMIASSLELGCGSYTHISLVPHIYYRRDADDIEPFCG
                     HGERFRPIPEVCRACGKCP"
     gene            1882..1954
                     /locus_tag="PKL26_05660"
     tRNA            1882..1954
                     /locus_tag="PKL26_05660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1916..1918,aa:Gln,seq:ctg)
     gene            complement(1977..>2411)
                     /locus_tag="PKL26_05665"
     CDS             complement(1977..>2411)
                     /locus_tag="PKL26_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HNS83216.1"
                     /translation="GAANGTTVSHVADYAETVYAVENAYAPMRDLLAVAARRKNIIPL
                     FADATKPHEYAPLVETVQMLFQDVAHPDQAGIALWNSLFLDTGGAAVIAIKTRSIDVS
                     RSPREVVDDFCSALEGSPLAVKDVIWLEPYHRDHAILVCRKQ"
BASE COUNT          507 a          669 c          690 g          545 t
ORIGIN      
        1 tgttccagcc ctggttggtt acctgcgggg cccagtacca gaggtcgctg ttgggcgcca
       61 ggccggtaag tggacgggag gtcgtcgtgg tctggaagag tgcggtgtag gttgcaccgt
      121 cggcagtctt cacgcggata ttgacgaatc ccttgcctgc tgcgtcggcc ctggcggtga
      181 cggagtaggc gttggtctca acccggaagt tcaggtagtt gcccggcggc acggacttgc
      241 cggtcacgtc cttccctgcg gtgttgtccc ataccttgac ggtaagtgcg gggtcaacga
      301 cgttgaagac ggctgctccc cgtactcctg tccactggta ccatgttccg accttcccca
      361 cgaagatctg cggggagacg tagtagctgg tcggagttcc tacggtgacg atgttggtcg
      421 gctgatcgcc tgtcgaagtc ccggatgccc agtatgcaat ctgggttgcg gctccgagtc
      481 cggcagatat gtcaagaccc tcttcaccga tgaacacatc tccgcctgca gggacgctca
      541 cgatcgtggc tgcggatgcg ggaagcatca gcacggccag cgcgaccaag gcgatcagtg
      601 caaggctaaa gcgcttaatc atcttttgtc ctccttaact catgtccaaa tcctgaacgt
      661 tcgaatccaa tgcacacgtc agaaccgcca tcgacaggtg tcgaacagag gttccaccat
      721 gctccatact ccgaataacg gaatatgatt caatatttgt ggaacactta atatatcctt
      781 tttggtcgat tcaggaagaa taaatcgttg agcaggcgct gaatccggca aagagttgga
      841 gatctccggt tttccacgtt caacgccatc ccacaccatg attcatcatc gtcgcacctg
      901 ccagactccc ttccatttta cgcgcaccag tgtatctctg aaaacagtga gccagattgt
      961 cacaatatta cctgggaaac aattgggacc tgtgcagtgt acattgggat cgatatccag
     1021 gtggctatta cgtttaaccc ctctccagta cattaactag ttttccctgc gccatattga
     1081 tacatgcgta ttatcaaggc ccctaccctg gcatctgccc acgaacaggt ggtgaggatg
     1141 atcctcgaga aaggatggac cctcgacaca gaggacaggg aagccactgt cgagttcgag
     1201 gaggtggcca tgcgggtgga acagccagga aacccgccca tggtcagccc gtgttcacgg
     1261 ttccagcagc gtttcatgga gcagtatgcc cgggacctta tcgaaggatc cgattccgta
     1321 ttcgagtatg attaccatga ccgtctcttt aactggggcg ggggactgat gagaggcggc
     1381 gggaatgagg tccatgtgga ccaggtcgcg tatatcgtcc acaagcttcg cgaggccccc
     1441 gcaagccgca gggcaatcgc cgtcacctgg aaccctgcga tagatgagaa ccttgatgac
     1501 tgcccctgcc tccagctggt ccagtgcgtc tcgcgcgacg ggctgctgca tatgaaggtg
     1561 gtctttcgca gcaacgacat gctgacggca gcgggtgcga acatgtttgc gctcgtctcc
     1621 cttcagcaga tgatcgccag ttccctggaa ctgggttgcg ggtcttacac ccacatttcc
     1681 ctcgtgccac atatctatta ccggagggat gcagacgata tcgagccgtt ctgcggacac
     1741 ggggagcggt tccggcctat ccccgaggtc tgcagggcct gtgggaaatg cccatgaggc
     1801 tgttggcagg aaccccgcac ggtgtttcag gtgaatcagt caggttaatt acggaaaagg
     1861 gcgaacaaag tagggtgcga tagcccggta gtgtagcggt caatcatgcg ggactctgga
     1921 tcccgcgaca gcagttcgaa tctgctccgg gctacttctt ctctcctgcg taattgctac
     1981 tgcttcctgc agaccaggat ggcatggtcg cggtgatatg gctcgagcca gatcacatcc
     2041 ttcaccgcaa gcggggatcc ttcgagagcg ctgcagaagt catccacgac ctcccgcgga
     2101 ctgcgggata catcaatgct ccttgttttt attgcgatta ctgcagctcc acccgtatcg
     2161 agaaaaagcg agttccagag agcgattcct gcctggtccg ggtgggcgac gtcctggaaa
     2221 agcatctgca ccgtctccac gaggggagcg tattcatgcg gctttgtggc gtctgcaaaa
     2281 aggggtatga tgttctttct ccttgccgcc accgcaagaa ggtcgcgcat gggagcgtag
     2341 gcattctcaa cggcgtatac tgtctccgca taatcggcaa cgtgtgatac ggtcgtacca
     2401 ttggcggcac c
//
LOCUS       DAOQVJ010000238         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53835_length_2409_cov_3.711980, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000238 DAOQVJ010000000
VERSION     DAOQVJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53835_length_2409_cov_3.711980"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKL26_05670"
     CDS             complement(<1..921)
                     /locus_tag="PKL26_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNS83217.1"
                     /translation="MSHATGMEDVSVLDASTLAKKEKTVFVSLVVDFILWIPDIIAAI
                     LAGSITMFADVVKCGNELLATFFAYLTIRKLARGGKGAYDYGMGKFETVTSIITGAVM
                     LISLVLVFYTAITRILAPEALHISGVALALVMMSIGVTVNTWLWFKNYRIAQKEYSPI
                     MESQWRLFRTKAFSDLSVLLALIFSIVLHQFWWAVYIDPFASMIIVGFLFTTGYRVIS
                     ASLPDLLDKTLDESLQITIIRELTEFFCEYEAFHGVRSRRSGSNVYIEIFLEFDGDRR
                     MCEVQEAIDRMKASLESKIPKSSVTIVPSSG"
     gene            1120..1452
                     /locus_tag="PKL26_05675"
     CDS             1120..1452
                     /locus_tag="PKL26_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNS83218.1"
                     /translation="MELQCENRGEIRVVHVAGRIDSSNADEFGRDIRGVITGGSRQLL
                     LDFAGVPYINSAGLRAVLVAAKNLKMPGDRCVICGLSPEVKEIFELAGFMNILRTYQS
                     VEDALSHW"
     gene            complement(1626..>2409)
                     /locus_tag="PKL26_05680"
     CDS             complement(1626..>2409)
                     /locus_tag="PKL26_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNS83219.1"
                     /translation="VTCFGYTGYRNAKFGRIEVHEAITGSAREVLIRTRDIAEEMGFS
                     VLHGIVDCLWVQGGPVAAFKERVERETRLHTVDAYDWLVFLPMADGTGAYTRYFGRLS
                     DGSLKVRGIAARRGDTPPFIRDVQEEMLARMQQARTPEEIAALREPLRERYRRAVEEL
                     PSARPAEMAVSRRISTLRYRHACAEASAVQACAAEGIALSPGMEVSYVVTDSRTWAVD
                     LDWKAARFDVGYYRKLLGKAWEEVIFAADKAAMREKRGEQVT"
BASE COUNT          479 a          753 c          723 g          454 t
ORIGIN      
        1 accgctgctc gggacgatgg tgaccgagct cttcgggatc ttggactcaa gcgacgcctt
       61 catccggtcg atcgcctcct gtacctcgca catgcgccgg tcgccgtcga attcgaggaa
      121 gatctcgatg tacacgttgc tcccgctcct ccgggagcgg acaccgtgaa atgcttcgta
      181 ctcgcagaag aactctgtga gttccctgat gatcgtgatc tggagcgact cgtcgagcgt
      241 cttgtcgagg agatcaggga gcgacgcaga gataacccgg tagccggtgg taaagaggaa
      301 gccgacgatg atcatcgatg cgaagggatc gatatagacc gcccaccaga actggtgcag
      361 cacgatagag aagatgagcg cgagcagcac cgagaggtcc gagaatgcct tggtcctgaa
      421 gagtcgccac tgcgactcca tgatgggaga atactccttc tgggcgatgc ggtagttctt
      481 gaaccagagc caggtattca ccgtaacacc aatgctcatc atgacgagcg cgagcgccac
      541 cccgctgata tggagggctt cgggtgcgag gatcctcgtg atcgcggtgt aaaagacaag
      601 gaccagggag atgagcatca ccgcgcctgt gatgatgctc gtcaccgtct cgaattttcc
      661 catcccgtag tcataggccc ccttcccgcc tctcgcgagc tttcgtatgg taagataggc
      721 aaaaaacgtg gcaaggagct cgttcccgca tttcaccaca tccgcgaaca tggtgatcga
      781 gccggcgagg atggcggcga tgatatcagg tatccagagg ataaagtcca cgacaaggct
      841 gacaaagacc gtcttctcct tctttgcaag ggtcgacgca tcgagaactg atacgtcctc
      901 catcccggtc gcgtggctca tctgcagtcc ttttctcttc gtatgcgctg aaaggagaaa
      961 atacccgcag aatggatgca gagtggggat gcaggatctt ccctgctgcc gcgcaccgga
     1021 agatccgggt cgatcgcccg ctcccgcccg ccaactttta tcgtgtgcca gattccagaa
     1081 ttttccatgc gaaaagggga ccccgccgga gagtgaacca tggaactgca atgcgagaac
     1141 aggggagaga tcagggttgt ccacgtggcg ggcaggatcg acagcagcaa cgcggatgaa
     1201 ttcggcaggg atatcagggg tgtgatcacg ggcggaagcc gccagctgct cctcgacttc
     1261 gcaggcgtcc cctacatcaa cagtgcaggt cttcgggccg tccttgttgc ggcaaagaac
     1321 ctgaagatgc ctggcgaccg ctgtgtcatc tgcggacttt ctcctgaagt aaaagagata
     1381 ttcgaactcg cgggattcat gaacatactc aggacatacc agtcggtcga ggacgccctc
     1441 tcgcactggt gagcgaggga gtgctgcgtt ccccgtcgcg ggggagtgta aaggggtagt
     1501 ctcttttccg cggaagaccg cacaagcccg tggcagtctg cagcctccgc cggcacacct
     1561 ccggttgctc cacccttccg gcaccccggt aggaatcgtc cggcccaggg ggctcccggg
     1621 caacgtcagg tcacctgctc cccgcgcttc tcccgcatcg ctgccttgtc cgcagcaaaa
     1681 attacctcct cccaggcctt cccgagcagc ttccggtaat agcccacgtc gaagcgggcg
     1741 gccttccagt caaggtcaac agcccaggtg cgtgaatcgg tgacgacgta gctcacctcc
     1801 atcccggggg agagggcgat cccctccgcc gcgcaggcct gcaccgccga tgcctcggcg
     1861 caggcgtggc ggtagcggag ggtgctgatc ctcctgctga ccgccatctc tgcagggcgg
     1921 gctgaaggga gctcctccac tgccctgcgg tagcgttccc ggagcggctc acggagggct
     1981 gcaatctctt ccggcgtgcg ggcctgctgc atacgcgcca gcatctcctc ctgcacgtcc
     2041 ctgatgaagg gcggggtgtc tccacggcgg gccgcgatcc cccttacctt gaggctcccg
     2101 tccgagagcc tgccaaagta gcgggtgtag gccccggtcc cgtctgccat cgggaggaag
     2161 acaagccagt cataggcgtc caccgtatgc aggcgggtct ccctctccac ccgttccttg
     2221 aacgcggcaa ccggcccgcc ctgcacccag aggcagtcca cgatcccgtg caggacggaa
     2281 aagcccatct cctcggcgat gtccctggtc cggatgagga cttcgcgggc gctgccggtg
     2341 atggcctcgt gcacctcgat cctcccgaac ttcgcgttcc ggtacccggt gtacccaaag
     2401 cacgtcacg
//
LOCUS       DAOQVJ010000239         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53872_length_2408_cov_4.912027, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000239 DAOQVJ010000000
VERSION     DAOQVJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53872_length_2408_cov_4.912027"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKL26_05685"
     CDS             <1..689
                     /locus_tag="PKL26_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS83220.1"
                     /translation="GVPTVHTKWNMPTAILAGDVLFASAFEFIAMTDAPDNAKVRAIS
                     MLGRTCVQICEGQHMDMSFESRDDVLEGEYLKMVEKKTGVLYAASAGIGGILAGGNPS
                     YVDALYHFGLGIGMAFQIQDDIIDLLAPPAVSGKDQASDLREGKKTLIAMKALEKGFD
                     LSEFRRDLSPAEVDAVIKKLESLGVLEEVRMSARDLIKNGKKRISILPDSDEKQLLSD
                     LADYFLSRSY"
     gene            692..2380
                     /locus_tag="PKL26_05690"
     CDS             692..2380
                     /locus_tag="PKL26_05690"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNS83221.1"
                     /translation="MEDNLRDILMVFALQNAVKHSSLPKPGTVIGMVMGKYPEFRSRA
                     REVSALLDEILSEVAALSPAEREHQLRLLAPDIADDMSRPRVHVKQLPGLEKAEHGVV
                     MRFAPNPSGPLHLGHARAAVLNDAYVKRYGGKYILRIEDTDPKRIDPEAYGMVIRDIE
                     WLGLTLHEVVYQSDRFDLYYRYGKELIERGGAYVCTCENEQFRQLKQEKTACPCRPLS
                     VEENLTLFDKMLAGEFFEGEASVRVRTDLDHPDPAMRDFPAFRILHQPLHPRIDAVVY
                     PLMNFSVAVDDHLLGVTHVIRGKDHIANTRRQRYIFDYFGWDVPVYRHYGRMGIEGVV
                     LSTSQMRKGIDSGDYEGWDDIRLGTLRALARRGISPEAVRQAVVEIGIGETDISFSWD
                     NLYAQNRMIVDPVANRYSFVPHPIGLDISDAPHHVAHALLHPNEPSRGDRILPFTGTV
                     LIPREELEKNPSLLRLKDLFNVKVSRDEQGYSLTYAGDQLNEARDAKASIIQWLPADC
                     ALPCVLRKPEGDVEGVCEPGVVREAGSVVQFERVGFARIDDTAGDRIVAYFTHR"
BASE COUNT          534 a          627 c          691 g          556 t
ORIGIN      
        1 gaggtgtccc cacggtccat acaaaatgga atatgccgac cgcaattctg gccggcgatg
       61 tgctctttgc gagcgccttt gaatttattg ccatgactga tgctcctgac aatgccaaag
      121 tacgggctat ctcgatgctc ggccggacct gtgtccagat ttgcgaaggg cagcacatgg
      181 acatgtcatt tgaatcccgg gacgatgtgc tggaaggaga gtatttgaag atggtggaga
      241 agaagacggg ggtcctctat gcagcttcgg caggaatagg tggaatcctc gctggtggaa
      301 atccttcgta tgtcgatgct ttataccact tcgggcttgg tatcgggatg gccttccaga
      361 tccaggatga tatcatcgat ctgctcgcac ctcctgcagt aagcggaaag gaccaggctt
      421 cagatctgcg ggaagggaaa aagaccctca ttgcgatgaa ggccttggag aaagggttcg
      481 acctttcaga attccgccgc gacctctccc ctgctgaggt tgatgccgtc ataaaaaaac
      541 tcgaatcact gggtgttctt gaagaggtca ggatgtcagc ccgtgacctt atcaaaaacg
      601 ggaagaaacg tatcagcatc ctccctgatt cagatgagaa acaactcctc tcagacctgg
      661 cagactattt cctttcccgg agttactgaa tatggaagac aatctccggg atatcctgat
      721 ggtttttgct ctgcagaatg cggtgaaaca ttcgagtctt cccaaaccgg ggacggtgat
      781 agggatggtc atggggaagt atcccgagtt ccgctcccgg gccagggaag tatctgcact
      841 cctggacgag atactctccg aggtagctgc cctctccccg gccgaacggg agcatcagct
      901 ccgtcttctg gcccctgata ttgccgacga tatgtcccgg cccagggtac atgtaaagca
      961 gcttccgggt cttgagaagg cagaacacgg cgtcgtgatg cgttttgccc cgaatcccag
     1021 cggaccgctg cacctggggc atgcacgagc cgccgtactg aatgacgcgt acgtaaagcg
     1081 atacggcggt aagtatattc ttcggattga agataccgat cccaaaagga ttgatcccga
     1141 agcgtatgga atggtgatca gggatatcga gtggcttggg ctcaccctcc atgaggttgt
     1201 gtaccagagc gatcgttttg atctctatta ccgttacggg aaggaactga tcgagcgcgg
     1261 gggagcctat gtctgtacct gtgaaaatga gcagttcagg cagctgaaac aggagaagac
     1321 tgcgtgtccc tgccgtccgc tctcggtaga ggagaatctc acgctttttg ataagatgct
     1381 ggctggagag ttcttcgagg gtgaagcttc cgtcagagtc cggactgacc ttgatcatcc
     1441 tgacccggca atgcgggatt ttcctgcgtt ccgtatcctt caccagcccc tgcatccccg
     1501 gatcgatgct gttgtctatc ccctgatgaa cttctcagtt gcagtggatg accacctcct
     1561 tggggtgacg catgtgatcc gtggcaagga tcacattgca aacacacggc gtcaacggta
     1621 tatttttgat tatttcgggt gggatgtccc ggtataccgt cactatggga ggatggggat
     1681 cgagggggtc gtgctctcca cctcccagat gagaaagggg attgactcag gagattatga
     1741 gggatgggat gatatccggc tgggcacgct cagagccctg gcccggcggg ggatctcccc
     1801 tgaagcggtc cggcaggcag tcgtcgagat tggtatcggg gagaccgata tctccttttc
     1861 ctgggataac ctctatgccc agaaccggat gatcgtcgat ccggtggcaa accggtattc
     1921 ctttgttccc cacccgatcg ggctggatat cagtgatgca ccccatcacg ttgcccatgc
     1981 cctgctccat ccaaatgaac cgtccagggg agaccggatc cttcccttca ccgggacggt
     2041 cctgattccg cgggaagagc ttgagaaaaa tccctccctc ctccgcttaa aagatctctt
     2101 caatgtgaag gtatcccgtg atgaacaggg gtactccctc acctatgccg gtgatcagct
     2161 caatgaagcc cgggacgcca aagcctctat catccagtgg ttgcccgctg attgcgcatt
     2221 accctgtgtg ctccgcaagc cggaaggtga cgttgagggg gtctgcgagc cgggtgtggt
     2281 gagggaggca ggatcggttg tacagtttga aagggtggga ttcgccagga tcgatgatac
     2341 cgcaggcgac cgaatcgtcg catatttcac ccaccggtaa agggaaaatc cctgttctcc
     2401 ctgccaac
//
LOCUS       DAOQVJ010000240         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_53877_length_2408_cov_3.892478, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000240 DAOQVJ010000000
VERSION     DAOQVJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53877_length_2408_cov_3.892478"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKL26_05695"
     CDS             <1..777
                     /locus_tag="PKL26_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNS83222.1"
                     /translation="GVVGDSLIRICQQGQSLTFEGADRKFIQTYFQLEEDLPGILASI
                     DRDPLVHSAICRCRGLRIVRQPGWECLASYIIATFANIPGIKRRISHLCRCFGNPFFG
                     SGGECYTFPSPVTIAFREPCRIAECRLGYRAPYLKETARLLAGDPGWEERLRELPYEA
                     ARRDLLRLRGVGKKVADCVLLFAFGKHEAFPVDVWIQRILATRYPGGESLRTYDQCSR
                     FGRSHFGEYAGYAQEYLFCDRAILAGEGRGDQVPVSQPER"
     gene            complement(747..1973)
                     /locus_tag="PKL26_05700"
     CDS             complement(747..1973)
                     /locus_tag="PKL26_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNS83223.1"
                     /translation="METGEKKMAWARQFMPVLSSIREEFIREMPFRGIRVGMALHVEA
                     KTACLVETLAAGGADVYITGCNPLSTQDDVAAALLRVKGVHCYAKRGATTGEYYAAID
                     RVLDARPQVTIDDGMDLIHRLHTARTDLLPDVIGGCEETTTGVHRLRAMAGDGKLRFP
                     VIAVNDTPMKRFFDNVHGTGESALSAIMLTTNMLIAGKAVVVAGYGFCGRGLARKLHG
                     LGARVIVTEVDPRRALEAYMDGFLVMPMDAAAERGEIFITTTGNTSVITARHFRVMRP
                     GAVLANAGHFNVEIDLQWLEANAGSVTRRDGIDTYRVDGKEIHVLAEGRLVNLAIPKG
                     MGHPIEVMDLSFALQALCARHIVSCGRSMSPGVYDVPSDIDDAVARKKLASLGLSIDE
                     LTPGQRNYLSGWETGT"
     gene            2109..>2408
                     /locus_tag="PKL26_05705"
     CDS             2109..>2408
                     /locus_tag="PKL26_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS83224.1"
                     /translation="MTLTRRIIPCLDLKGGRVVKGTHFLDLRDAGDPVELAQRYNEQG
                     ADEVVFLDITASKEQRGTIIEVIERAADQLFLPLTVGGGIRSVEDIQQILRAGADK"
BASE COUNT          450 a          770 c          720 g          468 t
ORIGIN      
        1 ggggtcgtag gagattccct tatccggatc tgccagcagg ggcagtcgct cacattcgaa
       61 ggggcagaca ggaagttcat ccagacctat ttccagcttg aggaggacct tcccgggatc
      121 ctcgcctcca tagaccggga cccgctggtc cattccgcca tctgccgatg ccgcggtctc
      181 cggatcgtcc gccaaccagg atgggagtgc ctcgcctcct acatcatcgc tacgtttgcc
      241 aacatcccgg gtataaagcg gcggatatct cacctctgcc gctgtttcgg gaaccctttc
      301 tttgggtcag gaggagagtg ctataccttc ccctctccag tgacaatcgc gttcagggag
      361 ccctgccgga tcgcggaatg ccgcctgggg taccgggcac cttacctcaa ggagactgca
      421 cggctccttg ccggagatcc gggctgggaa gaacggctcc gggaactccc gtacgaagcg
      481 gcgcgccggg atctcctccg cctcaggggg gtcgggaaaa aagtcgccga ttgcgtgctc
      541 ctctttgcat tcggcaaaca cgaggcgttc cccgtggatg tctggatcca gcgcatcctg
      601 gcgaccagat atcccggagg agagtccctc cgcacgtatg accagtgcag caggttcggg
      661 aggtcccact ttggggagta tgccgggtat gcgcaggaat atctcttctg cgaccgggca
      721 attcttgccg gagagggcag gggggatcag gtccccgtct cccagcctga aaggtaattc
      781 cgttgtccgg gagtgagctc gtcgatagaa aggccaagcg atgcgagttt cttcctcgca
      841 accgcatcgt cgatatccga agggacgtcg tagaccccgg gggacatgct ccgcccacag
      901 gagacgatat ggcgggcaca gagcgcctgc agggcaaagc tcaggtccat gacctcgatg
      961 ggatggccca tccccttggg aattgccagg ttcaccagcc ggccctccgc gaggacatgg
     1021 atctcttttc cgtccacgcg gtaggtgtcg attccgtcac gccgggtcac ggaccctgcg
     1081 ttcgcctcca gccactggag gtcgatctca acgttgaaat gcccggcatt cgccagcacc
     1141 gcaccgggcc gcattacccg gaagtgccgg gcggtaatca ccgaagtgtt ccctgtggtg
     1201 gtgatgaaga tctcccccct ttccgcggcc gcatccattg gcatgacgag aaacccgtcc
     1261 atgtaggcct cgagcgcacg acgcgggtcc acctctgtga ctatgacccg ggcgccgaga
     1321 ccgtggagtt tgcgtgcaag gcccctcccg cagaagccgt accccgcgac cacgactgcc
     1381 ttcccggcga tcagcatgtt cgtggtgagc atgatggccg agagtgcgct ctcaccggtg
     1441 ccatggacat tgtcaaagaa ccgtttcatc ggggtgtcgt tcaccgcgat gaccgggaac
     1501 ctgagcttcc catcgcctgc catggccctg agccggtgca ccccggtggt ggtctcttcg
     1561 cacccgccga tgacgtccgg gaggagatcg gtcctcgcgg tatggagacg atggatgagg
     1621 tccatgccgt cgtcgatagt cacctggggg cgggcatcca gcaccctgtc gatggccgcg
     1681 taatattccc ctgtcgtggc accgcgcttt gcatagcagt gcacaccttt cacccgcaaa
     1741 agcgccgcgg caacgtcgtc ctgcgtgctg agagggttgc aaccggtgat atatacgtca
     1801 gcgccgccgg ctgcaagcgt ctccacgagg cacgcggtct ttgcttcgac gtggagggcc
     1861 atgcctaccc gtatcccccg gaacggcatt tccctgatga actcctcgcg tatggaggag
     1921 agcaccggca tgaactgacg ggcccatgcc atcttcttct ctccggtctc catggaacac
     1981 accatccctc cggggagatc acaccagtcc ccggcatttc tcttaagaaa ttccgtctgc
     2041 agagtgataa aaaccccggg tcgcgcatgt cataatcctc ttatcccctc cgcacccaca
     2101 ctctttccat gacgcttacc cgtcggatca tcccctgcct tgacctgaaa ggcgggaggg
     2161 tagtgaaggg gacccacttc ctggatttga gggacgcagg ggacccggta gagctggcgc
     2221 aacggtacaa cgaacagggt gctgacgagg tggtcttcct tgacatcacc gcgtcaaagg
     2281 agcagcgcgg gaccatcatc gaggtgatcg agcgggccgc cgaccagctc ttcctccccc
     2341 tcactgtcgg tgggggaata cgctcggtcg aggatatcca gcagatcctc cgcgccgggg
     2401 ccgacaag
//
LOCUS       DAOQVJ010000241         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54144_length_2401_cov_2.680733, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000241 DAOQVJ010000000
VERSION     DAOQVJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54144_length_2401_cov_2.680733"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKL26_05710"
     CDS             <1..1317
                     /locus_tag="PKL26_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNS83225.1"
                     /translation="GRIVHANPMFQKLLGHPLEVITSRPFTEFLHPDDCEMVMDYHRQ
                     RVRGESPPPEYSFRIITGEKQGRWFRINSTPITWSGKPATLGFIVDITEQKQATEALK
                     ESEQRYIALFDHNYSVSLLIDPDTGRIVGANDAACTYYGYPREDLLKKGIYELNLLPK
                     EKVVNDLIRAKNEREKHFFATHYLANGEKRNVEIYSGPITIHGKPLFYSIIHDITARK
                     RAEEALRKAHRQMQLLSGITRHDILNSITVAEGYLSLLDDQDTDHYETYKANLNQALQ
                     RIKRQIEFTKEYENLGSQEPCWQDLDLLLGSLDIPHSLDFHIDRCNMKVFADPMLPKV
                     FENLLENSLRYGGQGMTTLRISCSASGDTLFVTWEDNGAGIPEEDKKKIFYRGYGKGT
                     GLGLFFASDILGVTGITLSETGIPGQGARFVLTVPSTGFRKKSKDL"
     gene            complement(1587..2051)
                     /locus_tag="PKL26_05715"
     CDS             complement(1587..2051)
                     /locus_tag="PKL26_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83226.1"
                     /translation="MPSDQSRAGVSETADFVEQSVTKTATRELTTSFADIAALTTVVN
                     SVISTNPFGCTAYESGGQSMDAVAKTREYYNGRVIFEDDDAKTTGYVTIRAPTGAAFA
                     TMKTEVISDAEMAAAMGGDASDDFERDTYSVILYYPMRIRIQSKSVEEEGSH"
     gene            2210..>2401
                     /locus_tag="PKL26_05720"
     CDS             2210..>2401
                     /locus_tag="PKL26_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83227.1"
                     /translation="MPDALKITYQGGRMEASLQVPDAILKLVERYEFHQAAYRRGQFN
                     ETQLRREFVDPFFKTLGWDV"
BASE COUNT          596 a          613 c          634 g          558 t
ORIGIN      
        1 ggtcggatcg tgcatgcgaa tcccatgttc cagaaactcc tcgggcaccc gcttgaggtt
       61 atcacgtcaa gacctttcac ggagtttctc catcctgacg attgtgagat ggtgatggac
      121 tatcaccggc aacgcgtacg gggggagagt ccgcctccgg aatattcctt taggatcatc
      181 acaggggaaa agcaaggccg gtggttccgg atcaactcaa ccccgatcac ctggtcggga
      241 aaacccgcaa ccctgggatt tatcgtcgat atcaccgagc aaaagcaggc cacggaagca
      301 ttaaaagaga gcgaacagcg ctacatcgcc ctgttcgatc ataattattc cgtctctctc
      361 ctgattgacc ctgataccgg ccggatcgtc ggcgcgaacg atgccgcctg cacgtattac
      421 ggctatcccc gcgaggatct cctgaagaag ggaatttatg agctgaacct gctgccgaag
      481 gagaaggtgg tgaatgatct catccgggcg aagaacgagc gggagaaaca cttctttgcg
      541 acgcattacc ttgcgaacgg cgagaaacgg aacgtggaga tctattccgg gccgatcacc
      601 atccacggaa agccgctctt ctattccatt attcacgata tcactgcccg gaaacgtgcg
      661 gaggaggcgc tccgcaaggc acaccgccag atgcagctgc tctccgggat tacccgccat
      721 gacatcctga acagtatcac ggttgcggaa ggatatctca gccttcttga tgaccaggat
      781 acggatcatt atgagaccta caaggcgaat ttgaaccagg cacttcagag aatcaagcgc
      841 cagatagagt tcaccaagga atatgagaac ctcgggtcac aggaaccctg ctggcaggac
      901 cttgatctgt tgctcggctc tcttgatatt ccccactcgc ttgatttcca catcgatcgg
      961 tgcaatatga aggtcttcgc cgatcccatg ctcccgaagg tctttgagaa tctccttgag
     1021 aattctctcc gatacggagg acaaggtatg acgactctcc ggatcagctg cagtgcttcg
     1081 ggagacacat tattcgtgac ctgggaagac aatggggcag gaattcctga ggaggacaag
     1141 aagaagatct tttaccgggg atacgggaag gggaccgggc ttggattatt tttcgcgtcg
     1201 gatatccttg gggtcaccgg tatcacgctc agcgaaacgg gaattcccgg ccagggggca
     1261 cggtttgtgc tgacagtccc cagtacaggt ttccggaaga aatccaaaga cctgtgaaaa
     1321 ccgctccttt ctcccgatga atgatgacag ggtggcccga tatccagcat attcactctc
     1381 ctgcgggtct tggtgcggtg tggatgtcgc atttctccga atcgatgaga atgaagcgaa
     1441 atcctaatgg tgtcaatagt tataccagtt gatgctgcag tgggttctag ttgccataca
     1501 aactgctgca gcggcatata gggtcaatag cgacaaaatc tcctaaaaca gcatggatca
     1561 cattgatgaa ttccccccaa ttctcatcaa tgtgaccctt cttcttcgac tgattttgat
     1621 tggatcctaa ttctcattgg atagtacagt atgacagaat aggtatcccg ttcgaagtca
     1681 tccgaagcgt caccgcccat tgcggcggcc atctcggcgt cgctgatgac ctcggtcttc
     1741 atggttgcga aggctgctcc agttggtgca cggatggtca cgtatccggt ggtctttgcg
     1801 tcgtcatcct cgaagatgac ccggccgttg tagtactccc gggtcttggc aaccgcgtcc
     1861 atggactggc cgccagactc gtatgcagta cacccaaagg ggttggtact gatgacggag
     1921 tttaccacgg tggtgagcgc agcaatatcc gcaaaggagg tggtaagctc tcgggttgcg
     1981 gtcttggtga cgctctgttc aacaaaatct gccgtttctg atactccagc ccttgattga
     2041 tcggagggca ataagggggt aagccaaaga aaaaaatata ttttcatcca gatggcaaat
     2101 ccagtcttgt caaatggcta aaatgcaaat tcacgctgat tgaagctgcg tgaagtgaac
     2161 aatatttccg gaatcttacg aaaatcgaat ggttatcgaa tgattcttcg tgcctgacgc
     2221 cctgaaaatt acataccaag gcggtcgcat ggaggcatca ctacaggtcc cggacgcaat
     2281 cctcaaactt gttgaaagat acgaatttca ccaggcggct tacaggagag gacagttcaa
     2341 cgagacccaa ctccggaggg agttcgttga tccgttcttc aagaccctcg gttgggatgt
     2401 c
//
LOCUS       DAOQVJ010000242         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54205_length_2399_cov_3.566126, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000242 DAOQVJ010000000
VERSION     DAOQVJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54205_length_2399_cov_3.566126"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKL26_05725"
     CDS             <1..709
                     /locus_tag="PKL26_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS83228.1"
                     /translation="AECDEIERRVTASRPDLTVFRIDCYRPETYLEDTARLAEIFGKE
                     EEGARLAAFYAGALAKVDAAASSSAPPEVYFETWNDFKSVARGSGYHDKVTMAGGANI
                     FRDNPAEYPEIDPEAVISRKPEVVIKLVGSSKYIFGGYSGENTTRFEDVRDTLLPRAG
                     WSSLPAVRDGRVYVLHNAIMGGPQYLIGVTYMARWFHPDSAGDLDPESLHRTYLQEFQ
                     GLDPGLARADRFVFPVK"
     gene            723..1784
                     /locus_tag="PKL26_05730"
     CDS             723..1784
                     /locus_tag="PKL26_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNS83229.1"
                     /translation="MDDEKEKIAGQYLRMQAKRLLFVGVLAVLIILLAVGSTIIGSAG
                     LTVEEVFTAVLAGLLPGSFSADPLAFTIVWDLRLHRVLFAVVAGFGLAIAGAVMQGVL
                     RNPLASPFTLGIASAATFGAAIAIIFVPTALSGEIALVVSAFMMSAFAAVAIYGLSRY
                     RGVSAESMVLAGIILMYLFGAMTSFLQYAGTTEQLQAVVFWMFGSLQNTSWEKLAIVT
                     LVVVVSTPLLVWRAWDLNALGMGDQVAQSLGVKVEQTRTVHMLVASLITASIICFTGT
                     IGFIGLVSPHITRLAIGGDHRYLIVASGFVGALILLGADSLARTILAPQIIPVGIMTA
                     FIGIPFFLYLFMRRKEEYW"
     gene            1781..>2399
                     /locus_tag="PKL26_05735"
     CDS             1781..>2399
                     /locus_tag="PKL26_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS83230.1"
                     /translation="MKLEVADLSFSYQDHRVLEEITLDVEGGELVGVLGPNGCGKTTL
                     IKCINHVHAPKGSVRFGGHDVARMGNTERARLFAYVPQALSIGMAMTVFESVLMGRRP
                     HVRWGVGDSDIGMVSAALERLKISHLAFRKVTQISGGERQKVVIARALVQDPMLLLLD
                     EPTSALDLRHQLEVMQIIRSVVDQKGIGGLMAIHDLNLAARYCDRV"
BASE COUNT          413 a          748 c          745 g          493 t
ORIGIN      
        1 ggcagagtgc gacgagatcg agcggagggt cactgcatcg aggcccgatc tcactgtctt
       61 caggattgac tgctaccgtc ccgaaaccta tctcgaagat acggcccgcc tcgcggagat
      121 tttcggaaag gaggaggaag gtgcccgcct cgcggcgttc tatgccggtg ccctcgcgaa
      181 ggttgacgcg gccgcctcgt cctccgcacc ccccgaggtc tacttcgaga cctggaacga
      241 tttcaagagt gtcgccaggg gctcgggcta ccatgacaag gtcacgatgg ccgggggagc
      301 aaacatcttc cgcgacaacc ctgccgagta tcccgagatc gatcccgagg cggtcatttc
      361 ccggaaacct gaagtcgtga tcaagctcgt gggctcctcg aagtacatct tcgggggata
      421 ctcaggggag aacaccaccc gctttgaaga cgtcagggac accctcctcc caagggctgg
      481 gtggagcagc ctccctgccg tccgggacgg gagggtgtac gtgctccaca acgcgatcat
      541 gggagggccg cagtacctga tcggcgtgac ttacatggcc aggtggttcc accccgacag
      601 cgcaggcgat ctggacccgg aatcactgca ccgcacgtat ctccaggagt tccagggcct
      661 cgacccgggc cttgcacgcg ctgaccgctt cgtgttcccg gtaaagtgag gagtgtgtgg
      721 tcatggatga cgagaaagag aagattgccg gccagtacct gcgcatgcag gcaaagaggc
      781 tcctctttgt cggggtcctc gcagtgctca tcatcctgct cgcggttggc tcgacaatca
      841 tcgggtccgc ggggctgacg gttgaggaag ttttcacggc ggtcctggcc gggcttcttc
      901 cgggttcgtt ctctgccgat cccctcgcat tcaccatcgt gtgggatctc cgccttcatc
      961 gggtcctctt cgctgttgtg gccggattcg ggctcgcgat cgcgggtgcc gtgatgcagg
     1021 gggtgctccg taaccccctc gcaagcccgt ttaccctcgg catcgcctca gcagcgacgt
     1081 ttggtgcggc gatcgccatc atctttgtcc ccaccgcgct ctccggcgaa atcgccctcg
     1141 tggtctctgc cttcatgatg agcgcttttg ccgcagtcgc gatctatggc ctctcccggt
     1201 accggggggt ctctgctgag agcatggtgc tcgcggggat catactcatg tacctcttcg
     1261 gcgccatgac ctcgttcctg cagtatgccg gcaccaccga gcagctccag gcagtggtct
     1321 tctggatgtt cgggagcctc cagaacactt cgtgggagaa actcgccatt gtcaccctgg
     1381 tagtggtcgt ctccaccccc ctgctggtct ggcgggcatg ggacctcaat gcgctcggca
     1441 tgggcgacca ggtggcccag agcctggggg tgaaggtcga gcagacccgg accgtccaca
     1501 tgctggtcgc atccctcatc actgcctcta tcatatgctt taccgggacc attgggttca
     1561 tcgggctcgt ctcccctcac atcacgaggc ttgccatcgg cggggaccac cgctacctga
     1621 tcgtagcatc gggctttgtc ggagccctca ttctccttgg tgcagacagc cttgcccgga
     1681 ccatcctcgc accccagatc attcctgtcg ggatcatgac ggcgttcatc ggcatcccgt
     1741 tcttcctcta cctcttcatg cgtcgcaagg aggagtactg gtgaagctgg aagtggcgga
     1801 tctttccttc tcctaccagg accatcgcgt cctcgaggag atcacccttg acgtggaagg
     1861 gggcgaactc gtcggcgtcc tcgggccgaa cgggtgcggg aagaccaccc tgatcaagtg
     1921 tatcaaccac gtccatgcac ccaaaggaag cgtccgcttc ggggggcatg atgtcgcccg
     1981 tatggggaat acagagcgag cgaggctctt tgcctatgtc ccccaggccc tctccatagg
     2041 gatggccatg acggtgtttg agtcggtcct gatggggagg aggccccatg tccgctgggg
     2101 agtgggggac tcggacatcg gaatggtaag tgcggcgctc gagcgcctga agatctccca
     2161 cctcgcgttt cggaaggtta cgcagatcag cgggggggag cgccagaagg tggtgatcgc
     2221 ccgggccctt gtccaggatc ccatgctcct tctcctcgac gagccgacga gcgcgcttga
     2281 cctccgccac cagctggagg tcatgcagat catccgatct gtggtggacc agaaggggat
     2341 cgggggcctg atggcaatcc atgacttaaa cctggcggcc cggtactgcg acagggtgg
//
LOCUS       DAOQVJ010000243         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54377_length_2394_cov_4.491236, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000243 DAOQVJ010000000
VERSION     DAOQVJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54377_length_2394_cov_4.491236"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKL26_05740"
     CDS             <1..415
                     /locus_tag="PKL26_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNS83231.1"
                     /translation="INAWNNEQWGKAYMDDEGYYWFVGRDDDIIKSSGYRIGPFEVES
                     ALLEHPAVAESAVVGSPDLIRGTIVKAFVVLKPGFEPSERLVKDIQTHVRKNTAPYKY
                     PRAIEFTNDLPKTLSGKIRRGELRVRELERFNSSR"
     gene            542..2215
                     /locus_tag="PKL26_05745"
     CDS             542..2215
                     /locus_tag="PKL26_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS83232.1"
                     /translation="MAAAEHNMHNYREVYDHFSLKVPEYYNFGFDQVDRLAAKDRNRL
                     AMIWVDQEGHEKTFTFRQMMNLSNSAANMLLKYGIKKGDRVIVMLTRVPEWWIFALAC
                     IKLGAVFCPCPTMLTGKDLKYRINAAKIRMVITDNENAPKVEEICTECPSLRSRLVID
                     ADLPGWISYREEQEYPAPVSRRLVTMSGMEKTRSSDPLVIYFTSGTTGEPKMVVHNHA
                     LPLGHVTTGKFWLDLRENDLHFTLADTGWAKSSWGKFFGPWTQGACILVYDIRGRFKA
                     TELLPILEKYEVTVFCAPPTVYRMLILADLKKYDLKELRHCVSAGEPLNPEVIRVWKE
                     GTGLTIYEGYGQTELVLVIGTFPCMEARPGSMGKPAPGWHIELQDDEGKPVPAGEVGR
                     IAIRVKPKPVGLFDGYLYDPEANEHAFIGDYYYTGDKAYVDEDGYYWFVGRDDDVIKS
                     SGYRIGPFEVESALLEHPAVKEAAVVGSPDVIRGMIVKAFIVLKEGFQPSEQLVKDIQ
                     KFVKKTTAPYKYPRAIEFVDDLPKTISGKIRRNVLREKEMQSGSPGGNL"
     gene            2302..2386
                     /locus_tag="PKL26_05750"
     tRNA            2302..2386
                     /locus_tag="PKL26_05750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2338..2340,aa:Leu,seq:cag)
BASE COUNT          529 a          703 c          708 g          454 t
ORIGIN      
        1 gatcaacgcc tggaacaacg aacagtgggg caaggcctac atggacgacg aggggtatta
       61 ctggtttgtc ggccgcgacg acgacatcat caagagttcc ggctaccgca tcggcccctt
      121 tgaagtggag tctgcactgc tcgagcaccc ggcggtcgcc gagtcggcgg tggtcggatc
      181 gcccgatctc atccgcggga ccatcgtgaa ggcattcgtg gtcttaaaac cagggttcga
      241 accctccgag cgcctggtga aggacatcca gacccacgtg cggaagaaca ccgctccgta
      301 caagtacccg agggcgatcg agttcacaaa cgatctccca aagacactct ccggaaagat
      361 caggcggggc gagcttcggg tacgggagct tgagaggttc aacagctccc ggtaattccc
      421 atcttttttg acctcatttt ctccggataa tctttctccc ctgcaaattc ctgattttga
      481 atgcctgatg cgagaaaaaa gagcatcttt ttgtcggttc tcgcacaaca tatcagttca
      541 tatggctgca gcggagcaca acatgcacaa ttaccgggaa gtctatgatc acttctccct
      601 caaggtccct gaatactaca acttcgggtt cgaccaggtc gaccgtctcg ccgcaaagga
      661 caggaaccgg ctcgcgatga tctgggtcga ccaggagggc cacgagaaga cgttcacctt
      721 caggcagatg atgaacctct cgaactccgc ggcgaacatg ctcctcaagt acggcatcaa
      781 gaagggtgac cgggtgatcg tcatgctgac aagggtcccg gagtggtgga tattcgcgct
      841 cgcctgcatc aagctcgggg cggtcttctg cccctgcccc acgatgctga ccggaaagga
      901 cctgaaatac cggatcaatg cggccaagat ccggatggtc atcacggaca acgagaacgc
      961 acccaaagtc gaggagatct gcaccgagtg cccgagcctc cgctcccgcc tggtgatcga
     1021 cgccgacctc ccgggctgga tcagctaccg cgaggagcag gagtaccctg cgcccgtctc
     1081 ccgcaggctc gtcaccatga gcgggatgga gaagacccga tcgagcgacc cgcttgtcat
     1141 ttacttcacc tccgggacga cgggggagcc caagatggtc gtccacaacc acgccctgcc
     1201 gctcggccac gtcacgaccg ggaagttctg gctcgacctc cgggagaacg acctccactt
     1261 taccctcgcc gacaccgggt gggccaagtc ctcgtggggc aagttcttcg ggccctggac
     1321 gcagggggca tgcatcctcg tgtacgacat ccgggggagg ttcaaggcga ccgagctcct
     1381 gccgatcctc gaaaagtacg aggtcacggt cttctgtgcc ccgccgaccg tgtatcgcat
     1441 gctgatcctc gccgacctga agaagtacga cttaaaagag ctccgccact gcgtgagtgc
     1501 cggcgagccc ctcaacccag aggtcatacg ggtctggaag gaggggacag gtctcaccat
     1561 ctacgagggc tacggccaga ccgagctcgt gctcgtcatc gggacctttc cctgcatgga
     1621 ggcgcgaccc gggtcgatgg gaaagcccgc acccgggtgg catatcgagc tccaggacga
     1681 cgaggggaag ccggtccctg caggcgaggt ggggaggatc gcgatcaggg tgaagccaaa
     1741 gccggtcggt ctcttcgacg gctacctgta tgaccccgag gcaaacgagc atgcgttcat
     1801 cggcgattac tattacaccg gcgacaaggc ctacgtggac gaagacgggt attactggtt
     1861 cgttggtcgt gatgacgacg tgatcaagag ttccggctac cgcatcggcc ccttcgaggt
     1921 tgagtccgcg ctgcttgaac atccggcggt aaaagaagcc gcagtggtgg gatccccgga
     1981 cgtgatcagg gggatgatcg tgaaggcctt cattgtctta aaagagggct tccagccgag
     2041 cgagcagctc gtaaaggaca tccagaagtt tgtgaagaag accaccgcac cctacaagta
     2101 cccgagggcg atcgagttcg tcgatgacct gcctaagaca atctcgggga agatccgtcg
     2161 aaatgtgctc agggagaagg agatgcagtc aggatccccc ggcgggaacc tgtgagcgga
     2221 gtaaagccgg gttctcattc ccatttttct ggccattgaa tccacatctt tattttcccc
     2281 gacgacgtac cagtaggagg agcgagggta gccaagccag gtcaaaggcg ctagattcag
     2341 ggtctagtct cgcaggagtt cttgggttca aatcccatcc ctcgcattcc tttt
//
LOCUS       DAOQVJ010000244         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54381_length_2394_cov_3.794784, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000244 DAOQVJ010000000
VERSION     DAOQVJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54381_length_2394_cov_3.794784"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKL26_05755"
     CDS             complement(<1..827)
                     /locus_tag="PKL26_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNS83233.1"
                     /translation="MIIDRIVMKNFKRFRQQEIRFRDGITGILGNNGTGKSSIVEAVF
                     FSLYGVQATGLPSDYIVSSFAGQKEKCEVRLDFRVGGDEYTVLRSFKKGKSVQHDASF
                     YRAGKLRATGVSQVEAEVKRTLGMGPVDFRNTVYAAQKDLLTLLEHTSGKRKEWFLRA
                     LGIDYLKTESDSLLKERVDAKERELRLREGELKGLTDRHDPGARPQMENAMKDSKARL
                     QDLSRQAEDHAAGRRQVARLLQEFSEKKTEHTRLVERHLALITEVSGLSRQRDQISTR
                     "
     gene            complement(892..2034)
                     /locus_tag="PKL26_05760"
     CDS             complement(892..2034)
                     /locus_tag="PKL26_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS83234.1"
                     /translation="MKLIHIADTHLGLSQFNRLDPESGINLREKQIYENFLSSVDRII
                     AAKPDVLVHAGDLFDTVKPKTRAYTTVLEALERLHVAGIPLVVIAGNHSMVKTRYTTS
                     PFEVLVYHPGDIFAAYNFRYEHVEIGDTMFHLIPNMLRPGDYRDAYDEVEISMNHHNV
                     LVTHGLATQVRDKRLATVAEHELDSTILSDSFDYIALGHYHCQSQITGNAWYSGSIEF
                     LTYGEISDSKGGLFIDLDPLSVCPFPLPMTPMIDLGTIQCDGVRPGEISGEIISRITE
                     RHILPFSMVQVTLDGLSREHGKGPDMRDLVNVRERLLDLKIRARSPEENRVVPLQQDV
                     KAINYLEEFATFLDIQQIGARQKEYVARCGHEVLTTVMERHREDAE"
BASE COUNT          499 a          655 c          653 g          587 t
ORIGIN      
        1 cgggtggata tttgatccct ctgacgagag agtcctgaca cctctgtgat aagtgcaagg
       61 tgccgctcaa cgaggcgggt atgctccgtc ttcttttcgg agaactcctg gagcagacgg
      121 gcaacctgcc gccttcctgc cgcgtgatcc tcggcctgtc gtgacagatc ctgcagcctg
      181 gctttcgagt ctttcattgc gttctccatc tgtggccggg cgcccgggtc atgccggtcg
      241 gtgagcccct tgagttcccc ctctcgcagc cgaagctccc gctctttcgc atcaacccgc
      301 tctttcaaga gactgtcgct ctcggtcttc aggtaatcga tgcccagtgc acgcaggaac
      361 cattctttcc tcttccccga tgtatgctca aggagcgtaa ggaggtcttt ctgggctgcg
      421 taaaccgtgt ttcgaaaatc gacgggcccc atcccaagcg tcctcttcac ttcggcctct
      481 acctggctca ccccggttgc acgaagtttt ccagcccggt aaaacgatgc gtcgtgctgg
      541 acgctttttc cttttttgaa cgagcggagg accgtatact cgtcaccacc gacgcggaag
      601 tcgagccgca cctcgcattt ctccttttga ccggcaaacg atgagacgat gtagtcggac
      661 gggaggcccg ttgcctgtac gccatagagg gaaaagaaga ccgcttccac gatactgctc
      721 ttcccggtcc cattgtttcc gaggatgccg gtaattccgt ccctgaaccg gatctcctgc
      781 tgcctgaacc gcttgaagtt cttcatgacg atccggtcga tgatcatccc atgcctccgt
      841 tcgggtaatc gtggtggaaa cggtctccag ggaaagcgtc tctttggggg gtcactctgc
      901 atcctcccgg tggcgttcca tcactgtggt cagcacctca tggccgcacc ttgcgacata
      961 ctctttctgc cttgcgccga tctgctgaat atcaagaaac gtggcgaact cctcaaggta
     1021 gttgatagcc tttacatcct gctggagggg caccacccgg ttctcttctg gagaccgggc
     1081 tctgatcttg agatcgagca gcctttcgcg gacattgaca aggtccctca tatcgggacc
     1141 ctttccatgt tcgcgggaga ggccgtcgag cgtgacctga accatcgaga agggtaggat
     1201 gtgcctttcc gtaatccgcg agattatctc gcccgagatc tcgcctggtc tgactccatc
     1261 gcactggatc gtcccaaggt cgatcatcgg ggtcatggga aggggaaaag ggcagacaga
     1321 gagcgggtca aggtcgatga aaaggcctcc tttcgaatcc gatatctctc cgtaggtgag
     1381 gaattctatt gatcccgagt accaggcatt gcccgtgatc tgcgactggc agtggtagtg
     1441 accgagcgcg atatagtcga acgagtcgga gaggatggta ctgtcaagtt cgtgctccgc
     1501 tacggtagca agtcgtttat ccctgacctg cgttgcgagc ccatgggtca ccaacacatt
     1561 gtgatggttc atagagatct ccacctcgtc gtatgcatcc cggtagtcac cgggtcgaag
     1621 catgttcggg atcaggtgga acatcgtatc cccgatctcc acgtgctcgt atcggaagtt
     1681 atacgccgca aagatatcgc ccgggtggta gaccagcact tcgaacgggg aagtcgtgta
     1741 ccgggtcttg accatgctgt ggtttcctgc aatgacgacg agggggatcc ctgcgacatg
     1801 gagtcgttcg agtgcttcaa ggacagtcgt gtaagcccgt gtcttcggct tcactgtgtc
     1861 gaaaaggtcg ccggcatgca caagcacgtc gggtttcgca gcgatgatcc tgtcaaccga
     1921 cgacaaaaaa ttttcatata tctgcttctc ccgcaggtta atcccggatt ccgggtcgag
     1981 ccggttgaac tgcgagaggc cgaggtgggt gtcggcgata tggatcagct tcatttctca
     2041 tgtttctcat tggactgtga tggttataag tgggaggggg aggggggtga aagtaatgct
     2101 gctattgggg gtcatcagca gtaaacctgc ataaatccct tatgacaata tcgaaaaaca
     2161 ggggatgaca caaggcccat tataaaatgc cccggattat cctccggaaa ccaagaatgt
     2221 tattctcgat ttcatcaaat cgtttgaatt gcacgtgttt tctctaaaat cttggatagc
     2281 taccgctacc agaccccccg ccgtttgagc gtgcctaact ggactagatt gagcgaaaat
     2341 gatgaaaaca agtttttgac atatgctctc tcaatagtcg tggcaaggac atga
//
LOCUS       DAOQVJ010000245         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54603_length_2388_cov_3.058723, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000245 DAOQVJ010000000
VERSION     DAOQVJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54603_length_2388_cov_3.058723"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKL26_05765"
     CDS             <1..226
                     /locus_tag="PKL26_05765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS83235.1"
                     /translation="DLHNLGPHYDRTLMAWHANFVNAWPRLSRRYDERFRRMWEYYLL
                     SCAGAFRARFIQVWQIVMTAQGTPQPSCRV"
     gene            324..719
                     /locus_tag="PKL26_05770"
     CDS             324..719
                     /locus_tag="PKL26_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNS83236.1"
                     /translation="MDLTDQQYWKSLINIGLTRILVLKVLSRGPNHGYGILKELESIT
                     TGCCVPTYGTLYPLLKELTLNGYAQVAEDRQLKGAQKRKVYTLTPSGMEAYRAALEAW
                     RSSVPYIYRAIEDDDLVFIKDIRRRLSKQ"
     gene            753..1781
                     /locus_tag="PKL26_05775"
     CDS             753..1781
                     /locus_tag="PKL26_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNS83237.1"
                     /translation="MFGLNNFITAGKFFLVIAGELVLIFVAVSFIIGILMEYLPPSKV
                     TDFLSNKLSWVQYLLGSGLGAITPFCSCSTVPLTAGLLKGGVPFGPTMAFLFASPVLN
                     PVIIALVLTLLGYKVMVVYVVVTLFGSMTAALLMSRLGMEAQVKPLLQVPTVCCGSDT
                     APVVRPLQTLPSAGCCAQQTEVPQASCCAVNLDWDGQNGSTQTAFKEKMKRASLSALD
                     TFKGVFWYLLLGAGIGAFIYGFFPQDLVVSLAGPGNPWSIPVAAVIGVPMYIRAETII
                     PISAALVGKGMGMGTVLALIIGGAGASIPELLILGSMFRKKLIVAFVANVFVVAIAAG
                     YLVDLLVY"
     gene            1884..2273
                     /locus_tag="PKL26_05780"
     CDS             1884..2273
                     /locus_tag="PKL26_05780"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS83238.1"
                     /translation="MQKEASLFKALSDQTRLRLVSILLFNGETCVCRLAQALNEPDFK
                     ISRHLGIMRSAGVVSARREGTWMYYRIVKPRSIFEQSCYEFLRKGMVNNHILKKDLQR
                     LSKTSCSNPGSPARKNESTYLKRRNDI"
BASE COUNT          523 a          669 c          667 g          529 t
ORIGIN      
        1 ggatttgcac aacctgggac cccattatga caggaccctc atggcctggc atgcgaattt
       61 cgtcaatgcc tggccgcgcc tgagccggcg gtacgacgag cggttcaggc gtatgtggga
      121 gtactacctg ctgagctgtg ccggtgcgtt ccgcgcccgg ttcatccagg tctggcagat
      181 cgtgatgacc gctcagggca caccccagcc ctcgtgccgg gtctgaatat acaatgacaa
      241 aatacttgac aagaagaggt gatactgcca tctgatatat agatagtcaa tatatataag
      301 atcattataa aagagagcca accatggacc tcaccgacca gcaatactgg aaatcgctta
      361 tcaatatcgg cctcacccgg atcctcgtgc tgaaggtgct gtcccgggga cccaaccatg
      421 ggtacgggat actgaaggaa ctggaatcga ttaccacagg ttgttgcgtg cccacctatg
      481 gaaccctcta ccctctcctg aaggagctga ccctgaacgg gtatgcgcag gtggcagaag
      541 accgtcagct caagggtgca cagaaaagga aggtctacac cctcaccccg tcaggaatgg
      601 aggcctaccg ggcggccctc gaggcgtggc ggtcctcggt cccctatatc taccgggcca
      661 tcgaagacga tgaccttgtg ttcatcaagg acatcaggag gcgtctttca aaacaataga
      721 gttcaagacc acagccatga aagggagtgc atatgttcgg cctgaacaat ttcatcaccg
      781 ccgggaaatt cttcctcgtg atcgcagggg agctcgtgct catctttgta gccgtatcct
      841 tcatcatcgg gatcctcatg gaatatctcc cgccgtctaa ggtgacggac ttcctgtcaa
      901 acaagctcag ctgggtgcag tacctcctgg gctcgggcct gggcgccatc acccccttct
      961 gctcctgctc gaccgtgccc ctgacggcag gcctgctcaa ggggggcgtc ccgttcgggc
     1021 cgaccatggc cttcctgttc gcctcgccgg tcctgaaccc ggtcatcata gcgctggttc
     1081 tgacacttct tgggtataag gttatggtag tatatgtggt cgtaacctta tttggttcca
     1141 tgacagcggc gctcctgatg tcgaggctcg ggatggaagc ccaggtcaag cccctgctcc
     1201 aggtccctac agtttgctgc ggtagtgata cggcacctgt agtcagaccc cttcagaccc
     1261 tgccttcggc aggctgctgt gcccagcaaa cagaggtgcc gcaagcctca tgctgcgctg
     1321 taaacctcga ttgggatggt cagaacggct cgacgcagac ggcattcaag gaaaagatga
     1381 aacgggcatc gttgtctgcc ctcgacacgt tcaagggagt gttctggtac cttctcctcg
     1441 gtgcgggaat aggtgcgttc atctacggct tttttccgca ggacctcgtg gtaagcctgg
     1501 caggacccgg caacccctgg tccatacccg ttgcggcggt catcggcgta cccatgtaca
     1561 tccgggccga gacaatcatc cccatcagcg ctgctcttgt agggaagggc atgggtatgg
     1621 gcacggtcct cgccctcatc ataggaggcg cgggagcgag catcccagag ctgctcatcc
     1681 tcggttccat gttcaggaag aagctcattg tagcctttgt ggcgaatgta ttcgtggttg
     1741 cgatcgcggc agggtacctc gtagatctcc tggtgtactg agaatccaga ttggcacctt
     1801 catgagtact tgacaggaga cgaagatttc atgatatatg catataaacg cagatatgca
     1861 aatatatcat ccgggataga acgatgcaga aagaggcttc cctgttcaag gcgctctcgg
     1921 atcagacacg actgaggctg gtgtccatac tcctgttcaa cggagagacc tgcgtgtgca
     1981 ggctcgccca ggcgctgaac gaaccggatt ttaaaatatc gcgccatctc ggtatcatgc
     2041 ggtccgccgg ggtcgtcagt gccaggcgtg agggtacgtg gatgtattac aggatcgtga
     2101 agccccgctc gattttcgaa cagtcgtgct atgagttcct gcgcaaaggc atggtgaaca
     2161 accatatact caagaaagat ctgcagcggc tttcaaagac ctcgtgctcg aatcctggct
     2221 cacccgcaag gaagaacgaa tcgacgtacc tgaaaaggag gaacgatata tgacggacgc
     2281 gacaggcaag aggctgtccc tgctggaccg gtttctcacc ctgtggatct tcctggccat
     2341 gggcttcggt atcctcctgg ggtacttctc gcccggggtc acccattt
//
LOCUS       DAOQVJ010000246         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54689_length_2385_cov_4.347639, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000246 DAOQVJ010000000
VERSION     DAOQVJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54689_length_2385_cov_4.347639"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="PKL26_05785"
     CDS             complement(<1..1458)
                     /locus_tag="PKL26_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNS83239.1"
                     /translation="MAGEEEDRRRFRFSVKTKILLVFLALSVSGLLVTGILAFVQIGD
                     VSRYAVESSSALGDRAVEDSTAAMERDARESLLRLAQDQAYISNIIFDRVSGEIEMLV
                     RYAGEIQADPSRVRPRHFYLQDEEPQDPASTTVLFLSPGVEKDIPFEERNAAGMMTDI
                     FIPLFASDRNLAAVYVGTESGMSFIYPWFTGMDATFDPRLRGWFRQAKETGAMTWSEP
                     YVDLLGHGLMMTCSKPVSNPTRGWFWVIGADVTVETINRNIIGTQVGESGYAMLIDQH
                     GNVITRPGLSSGDMRWDESFVTENLLESGNAGLVAVARDMTAGKEGVARVSFEGGDRF
                     IAFAPVKSVNWSVGVVMPVDEVIAPALSTRQRILGASEETASHITRQQEQLKTAFTAA
                     FLILLVVVIVLSLVFSRHLTRPLEDLKRGSEAVGRGDLDYQVEIRTGDEFEDLAVSFN
                     RMAGDLKEHIQSLRLTTAEKERIQKELDRVRQDYEN"
     gene            1622..2119
                     /locus_tag="PKL26_05790"
     CDS             1622..2119
                     /locus_tag="PKL26_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNS83240.1"
                     /translation="MSGSISFVQLEVKKPEDVNIILGHSHFIKTVEDLYEAMVGSVPG
                     ARFGIAFCEASADCLVRRDGNDEELCDMAEKNALAIGAGHSFIICMRDCYPINVLNAI
                     KSLQEVCTIYCATANSVDVIVAESPRGRGIMGVIDGSSPRGVETSENTRERIDLLRRF
                     GYKRG"
     gene            2202..2357
                     /locus_tag="PKL26_05795"
     CDS             2202..2357
                     /locus_tag="PKL26_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83241.1"
                     /translation="MNSYKYSRSRLSFTMVGMGFRNKAGCRGGRFAWGEENSRIELED
                     AGRGART"
BASE COUNT          487 a          700 c          706 g          492 t
ORIGIN      
        1 gttctcgtag tcctggcgga cgcggtcgag ctccttctgg atccgctctt tctccgcggt
       61 agtgaggcga agggactgga tgtgctcttt taagtcccct gccatccgat tgaacgagac
      121 tgcaaggtcc tcgaactcgt cgccggtacg gatctccacc tggtagtcga ggtcacccct
      181 cccgacggcc tccgagcccc tcttcaggtc ttcgagcggc cgggtgagat gccgggaaaa
      241 gacgagggag aggacgatta ccacgacgag aaggatgagg aatgcagcgg tgaatgccgt
      301 cttcagctgc tcctgctgcc gggtgatgtg cgacgcggtc tcttccgatg ccccgaggat
      361 gcgctgccgg gtggagagcg cgggtgcgat cacttcgtcg accggcatca ccacccccac
      421 gctccagttc acgctcttga ccggtgcaaa cgcgatgaac cggtcgccgc cctcaaaact
      481 gacacgtgca acgccctcct tcccggcagt catatccctc gcaaccgcca cgagcccggc
      541 attcccgctc tcgaggaggt tttcagtcac aaatgactcg tcccagcgca tgtcgcccga
      601 tgagagcccg ggcctcgtga tcacgttccc atgctggtcg ataagcattg cataaccgga
      661 ttcccccacc tgggtaccga tgatattcct gttgatggtc tcgacggtca cgtccgcccc
      721 aatcacccag aaccaccctc tcgtggggtt ggagacgggt tttgagcagg tcatcatcaa
      781 cccgtggccc agcaggtcaa cgtatggttc ggaccaggtc atggccccgg tctcctttgc
      841 ctggcggaac catcccctca gccggggatc gaatgtggca tccatgccgg tgaaccaggg
      901 gtagatgaat gacattccgc tctcagtccc cacgtagact gccgcaaggt tcctgtccga
      961 tgcaaagagc gggatgaaga tatcggtcat catcccggcg gcgttgcgct cttcgaaggg
     1021 gatatccttc tcgactcccg gtgagaggaa gaggacggtg gtcgaggcgg ggtcttgtgg
     1081 ctcctcgtcc tggaggtaga aatgccgggg cctcacccgg gatggatctg cctggatctc
     1141 cccggcataa cgcaccagca tctcgatctc ccctgaaacg cggtcgaaga tgatgttgct
     1201 gatatacgcc tggtcctgtg caagccggag cagggattcc cgtgcatccc gctccatggc
     1261 ggcagtgctg tcctcgacgg cccggtcacc aagggcgctg ctgctctcca cagcataccg
     1321 gctcacgtca ccgatctgga caaatgcgag gatacctgtg acaagcagtc cgcttacaga
     1381 gagggccagg aatacaagga ggatcttggt cttcactgag aaccggaacc gcctcctgtc
     1441 ctcttcctcg ccggccataa ggatcacgtc tgttccggga gggaataagc cagccggttt
     1501 gcacagctgc cccgcccttt tctgcgagaa tgctccaatc cggtatgccc gggtcaggag
     1561 aagtgtgaga tgccggcaat acttttcatc cagggattcc catcactcca ccgtgagaga
     1621 gatgtcagga agtatctcgt ttgtccaatt agaggttaag aaaccagaag acgtgaacat
     1681 catcctcggt cattcccatt tcatcaagac agtcgaggac ctgtacgagg caatggtggg
     1741 atccgtcccc ggtgccaggt tcggcatcgc gttctgcgag gcatccgcag actgcctcgt
     1801 tcgacgcgac gggaacgacg aagagctctg cgacatggcg gagaagaatg cactggcaat
     1861 tggggcgggg cattcattca tcatatgcat gcgggactgc tatcccatca atgtcctgaa
     1921 cgcaatcaag tcactacagg aggtctgcac aatatactgc gccacagcaa actctgtgga
     1981 cgtgatcgtg gcggagagcc cgcgtgggag ggggatcatg ggagtcatcg atggctcttc
     2041 gcccaggggg gttgagacct ccgagaacac gcgggagaga atcgatctgc tccgaaggtt
     2101 cggctacaag aggggatgag gtccccccct gcgtcctgat agtcgttttt catgatccgc
     2161 aggtgggcct tgttttgttc tcctcatgtg agggaggaaa aatgaacagc tacaaatact
     2221 cccgctcccg tctctctttt accatggtgg gaatgggttt ccggaataag gccgggtgca
     2281 ggggaggtcg ttttgcatgg ggtgaagaga acagtcgtat cgagctcgaa gacgcaggcc
     2341 ggggggctcg cacatgaacc ttcccggcca gttcaatggc caaaa
//
LOCUS       DAOQVJ010000247         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_54894_length_2380_cov_3.772473, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000247 DAOQVJ010000000
VERSION     DAOQVJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54894_length_2380_cov_3.772473"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..457)
                     /locus_tag="PKL26_05800"
     tRNA            complement(375..457)
                     /locus_tag="PKL26_05800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Leu,seq:tag)
     gene            1182..>2380
                     /locus_tag="PKL26_05805"
     CDS             1182..>2380
                     /locus_tag="PKL26_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNS83242.1"
                     /translation="MKRLETLAMIAHDMGVIFEFLGFVTLIPLLVLVIFGEWDMVVPM
                     ASVPLTFVILGLLISRIPRRDYIPQLSVALAAVALIWLAIAVVGSLPFVLGLHMTWTD
                     SIFEAMSGWTDSGITMIRSLDTTPRTLLFYRSFMQWIGGIGVIAFGIAMLSRSSLTQH
                     QLYRSEGRSEALMPNVVSTGRRMWVIYLFLTILFTGLVMLSGIPLWDALNLAMVGIST
                     GGFSIHDAGISFYDNALLEAIMIPVMIAGALPFKLYFFMYRGRIRPFLQDRVVWLILS
                     LILVGSLVITLDLHVFNSFPLETAVRQGVFCTASAVTSTGLQNSDLHWVAAPLIVITL
                     LMMIGGASGSTAGGIKANRVILGYEGLVWWFRRFFARGRAIVPFRHEGKAIPRQISEV
                     ELSKNML"
BASE COUNT          513 a          661 c          646 g          560 t
ORIGIN      
        1 tcggcgactc ccgccttgcc gtgtctcatg atatagaggt ccatgagagg agaggggtat
       61 ggctggttaa aaaaacattc gggttcccgg gatgcaaaag gggatgcagg tgagacatca
      121 caggaagaag aggcgatacg acaggtatga taggcgctcg accgggcatc cctggatcga
      181 gatggtactc ccgcgcgaga tacgccccgg caaagagaga ggcaccgtat acgataattg
      241 cgggcgatcg ataccctgga atccatcttg tgccgttgcc cggaaaatgg ctctttgtgg
      301 tgtgactgga attgacctgg agtcggaagg atcccaaaaa aagaagattt acaacgcaga
      361 acccgaaata ccgatgcgag ggaggggatt cgaacccccg aactcctgcg agaatggacc
      421 ctaaatccat cgcctttgac cgctcggcaa ccctcgctct cctcttacta ggatccgggg
      481 ggtgaaaaaa ccctttgcca tgccgtgggg tctccaggat cacattttgc gcgaatggtt
      541 ctcattgcat atccaatgga tcggagaacg ggaggtaatg tccctttcaa tccaattcat
      601 ctatcgcaaa actattattc tgcgcaatta ttatatacgt ggagatttaa tagaaatgtg
      661 ccatatatga aggtctggga tcatatacca ggccgcagac cttgtcagat cagcaccagc
      721 aaggtcatgg catcccggcc aaccgcgtga ggaacatggt aagagacgcc atcatggaga
      781 agacagggca tgggaaaaca cctgaccgtt cgccaccttt cggtcaggac atgcccgatg
      841 gcgattattc cctcctacga gcgaaaattc ttgccaaact ggatgatgag gacatacggg
      901 atgcactcgc aggcctgaac tccaggcctt tgtaacttca tatatggtat cttttctgct
      961 ttttatggca gaaaagagcg gaaccctggc attccgtgca gccaaatgcc cgggtcgcac
     1021 tcatatgacc ctctaaaaac tccccttttc ctggctcttc cccaagtgat ctgtaaccca
     1081 atcagtgccc ccgcagtttc gaggtatcga ggtcgtgttc ctgccaaacc gggcgcacca
     1141 ggggagaatc aagataatct cctgccgcca atgacctgat catgaagcgg ctggagaccc
     1201 tggcgatgat cgcccatgac atgggggtga tcttcgagtt cctgggtttt gtcaccctga
     1261 tccccctcct tgtcctcgtc atcttcgggg agtgggacat ggtggtgccg atggcctccg
     1321 tgccgctgac attcgtgatc ctcggtctcc tcatctcccg aattccaagg cgggactaca
     1381 taccacagct ctcggttgcg cttgccgcgg tggccctcat atggcttgcc attgccgtgg
     1441 tcgggagcct cccgtttgtt ctcggcctcc acatgacctg gaccgacagc atattcgagg
     1501 ctatgtccgg gtggaccgat tcagggatca cgatgatccg ctccttagac accacgccaa
     1561 ggactcttct tttctatcga tcattcatgc agtggatagg tgggatcggg gtaatcgcct
     1621 ttggtattgc gatgctctcg aggtcaagcc tcacccagca ccagctctac cggtctgaag
     1681 gcaggtcaga agctctcatg ccgaatgtgg tctcaaccgg gagacggatg tgggtcattt
     1741 acctctttct caccatcctc ttcacgggac tggtgatgct ttccggcatc cctctgtggg
     1801 acgctctcaa cctggcgatg gtcggcattt ccaccggggg tttctccatc cacgacgcgg
     1861 ggatctcgtt ctatgacaat gcgcttcttg aggccatcat gatcccggtg atgattgcag
     1921 gggctctccc atttaagctc tattttttca tgtacagggg gaggatcagg ccttttctcc
     1981 aggacagggt cgtatggctc atcctctccc tgatccttgt cgggtcgctc gtgatcactc
     2041 tggatctcca tgtcttcaac agcttcccac tggaaaccgc cgtgcgacag ggagtgttct
     2101 gcactgcatc cgcggtgaca tccaccgggc tccagaattc tgacctccac tgggtcgcag
     2161 caccgctcat cgtcatcact ctcctgatga tgatcggggg ggcatccggg agcactgccg
     2221 ggggtatcaa ggccaaccgc gtcatcctcg ggtacgaagg gcttgtctgg tggttcaggc
     2281 gcttttttgc cagggggagg gcgattgtcc cgttcaggca cgagggaaag gccatcccca
     2341 ggcagatctc cgaggttgag ctctccaaga acatgctgat
//
LOCUS       DAOQVJ010000248         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55322_length_2369_cov_4.267934, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000248 DAOQVJ010000000
VERSION     DAOQVJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55322_length_2369_cov_4.267934"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKL26_05810"
     CDS             <1..686
                     /locus_tag="PKL26_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNS83243.1"
                     /translation="SVGGVTKGSGMIAPNMGTMLAFIYTDAEVPAPALREALKAAARR
                     TFNRVVVDGDMSTNDIALCTATGKAGRVNPREFSQALEECCRMLAIQIAEDGEGASKL
                     LEVTVKGAPGEEEAATVAKTVVGSPLVKTAVYGADPNWGRVIAAAGRAGVEFDPDCVN
                     LWMGNGTESVPLVESGEIVVDLARAKALMKGKKVLFVLDLAAGDGEATAWGCDLTEKY
                     VEINGRYTT"
     gene            683..1555
                     /gene="argB"
                     /locus_tag="PKL26_05815"
     CDS             683..1555
                     /gene="argB"
                     /locus_tag="PKL26_05815"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNS83244.1"
                     /translation="MKREEVLTEALPYIQQFHGKTMVIKLGGHAMVDAAILETAIRDA
                     VLLQLVGIRVVLVHGGGPEITDKMKAMGKEPKFVAGLRITDEETLEIAQMVLVGKINS
                     NIVSLIAKSGGKGVGLSGNDGNLIIARRMDPQKVHVAGREQEVDLGHVGEIEEINPAV
                     LFTLLDRGYIPVVAPIAIDRSGQSLNINADTAAGEIAISLEAYKLINMTDVDGIMDAS
                     RTTVYRRLRVNEAEQLMGSGVISEGMIPKVSSLVRAVNSGVSFGHVVNGNIAHNLLLE
                     MFTDEGVGTMICAR"
     gene            1560..>2369
                     /locus_tag="PKL26_05820"
     CDS             1560..>2369
                     /locus_tag="PKL26_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83245.1"
                     /translation="MGTTRPEVRKKRLSNLCKLVISLIFVTLFLANAWADSPGPAPEG
                     GEKRESPIPSTLLYEDGKPVVHFFYNTYCTECQKTVPYMQEIAPLYPGVVFRFHDIRE
                     SDEARILFQEFKQQYGREFLPVPSLFLGPYVLSGYEEITANLGDAIRLTMMQGEVRPT
                     IPVPEVTGTKEVLTIPLVVGAALVDGINPCAFAVLIFLVLSLIALDTRKRVVTVGATF
                     ILAVFCFYFLSGLGLFAIVQSSGMSLLISVAAAAIAILAGVISIGEALWKRK"
BASE COUNT          497 a          657 c          769 g          446 t
ORIGIN      
        1 tctcggtggg cggggtcaca aagggttctg ggatgatcgc ccccaacatg gggacgatgc
       61 tcgcattcat ctacaccgac gcggaggtgc ccgcccccgc acttcgtgag gcgctcaagg
      121 ctgcagccag gcggacgttc aaccgcgtgg tggtggacgg cgacatgagc accaatgata
      181 ttgcgctctg caccgcgaca ggaaaggcag gacgcgtcaa tccacgcgag ttctcgcagg
      241 ccctcgagga gtgctgccgg atgctcgcca tacagatcgc tgaagatgga gagggtgcaa
      301 gcaagctcct ggaggtcacg gtgaagggag ccccgggaga ggaggaggcg gccacggtgg
      361 caaagacggt ggtcgggtca cccctcgtga agaccgcggt gtacggcgcc gatcccaact
      421 ggggacgggt aattgccgcg gcggggaggg caggcgtgga gtttgacccg gattgcgtga
      481 acctctggat gggaaacggc acggaatcag tgccgctggt ggaaagcggc gagatcgtcg
      541 tcgacctcgc ccgtgcaaaa gccctcatga agggaaaaaa ggtcctcttc gtcctggatc
      601 tcgccgcggg agatggggag gccacggcgt ggggttgcga cctgacggag aagtacgtgg
      661 agatcaacgg gaggtatacg acatgaaacg agaggaagtc ctgactgagg ccctccctta
      721 tatccagcag ttccatggaa agacgatggt gatcaagctg ggggggcatg cgatggtgga
      781 tgcggcaatc ctcgagactg ccataaggga tgcggtgctc ctgcagctgg taggcatccg
      841 cgtcgtcctg gttcacggcg ggggtcccga gattaccgac aagatgaagg ccatgggaaa
      901 agagcccaag ttcgtggccg gccttcgcat caccgacgag gagacactcg agatcgccca
      961 gatggtcctt gtaggaaaga tcaacagcaa cattgtctcc cttatcgcaa agagtggagg
     1021 aaagggggtg gggctctctg gcaacgacgg aaacctcatc atcgcgagga ggatggaccc
     1081 ccagaaggtc catgtcgcag gcagggaaca ggaggtcgac ctcgggcacg tgggggagat
     1141 cgaggagatc aacccggccg ttctcttcac gctccttgac cggggataca tcccggtggt
     1201 ggcgcccatc gctatcgacc ggagcggcca gagcctgaac atcaacgccg ataccgcggc
     1261 aggcgagatt gctatctcgc ttgaggccta caagctgatc aacatgaccg atgtggacgg
     1321 catcatggat gcctcgagga ccacggtcta caggaggctc cgggtcaatg aggccgagca
     1381 actgatgggg agtggcgtga tctcggaagg gatgattcca aaggtcagct ccctcgtgag
     1441 agcggtgaac agcggtgtct cgttcgggca cgtagtgaat ggcaacatcg cccacaacct
     1501 cctcctcgag atgttcactg acgagggcgt ggggaccatg atctgcgccc gctagggaga
     1561 tgggcacgac gaggccggaa gtgcgcaaaa aaaggttatc aaacctctgt aaactggtta
     1621 tttccctcat attcgtcact ctattcctcg cgaatgcctg ggcggattct cccggcccgg
     1681 cccctgaggg aggggagaaa agagagtcgc cgattccttc caccctcctt tacgaggatg
     1741 gaaagccggt agtccacttc ttctacaaca cgtactgcac tgaatgccag aagaccgtcc
     1801 cctacatgca ggagatcgct cccctctatc ccggggtcgt cttccggttc cacgacatcc
     1861 gggagagcga cgaggcccgg atcctcttcc aggagttcaa gcagcagtac ggccgggagt
     1921 tcctgccggt tccttcgctc ttcctgggcc cctatgtcct ctccgggtac gaggagatca
     1981 cggccaacct cggcgatgcc atcaggctta ccatgatgca gggggaggtt cgtccgacca
     2041 ttcccgttcc cgaagtcacc gggaccaaag aggtgcttac aattccgctg gttgtcggcg
     2101 cggcgctcgt ggatggaatc aacccctgtg cgtttgcggt gctgatattt ctcgtcctct
     2161 ctcttatcgc ccttgatacc aggaagaggg tggtgacggt gggggcaacc ttcatcctgg
     2221 cggtcttctg cttctacttc ctctcgggcc tgggtctctt cgcaatcgtc cagtcatccg
     2281 ggatgtctct tctcatctct gtggcggcag cggcgatcgc aatcctggca ggggtgataa
     2341 gcatagggga ggcattgtgg aagagaaaa
//
LOCUS       DAOQVJ010000249         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55367_length_2368_cov_3.694769, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000249 DAOQVJ010000000
VERSION     DAOQVJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55367_length_2368_cov_3.694769"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..812
                     /locus_tag="PKL26_05825"
                     /pseudo
     CDS             1..812
                     /locus_tag="PKL26_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313841.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
     gene            794..1606
                     /gene="folP"
                     /locus_tag="PKL26_05830"
     CDS             794..1606
                     /gene="folP"
                     /locus_tag="PKL26_05830"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNS83246.1"
                     /translation="MHRCTIGGLTVGDGSPVRLMGVINCSPESFYSESYTPAGDVFSK
                     ACQLVEQGADIIDVGARSTAPGAPPLTRGEEAARMVQALSVLEGSGIRVSVDTMDPAV
                     LEACLEYDIAAINDISGLLNPEYAAGVSGSGLPALLMASKWAVGDACSLDETHRALSM
                     VETRAHASGVTEYVLDPAIGLWQASRTADLDWELCRQFRQFSVYQRPLLAAVSRKSFL
                     GILVGKPPAGRLAASIAMASLLVCMGADIVRTHDVAETRDAILVAEKMRRWQ"
     gene            1603..>2368
                     /gene="cofE"
                     /locus_tag="PKL26_05835"
     CDS             1603..>2368
                     /gene="cofE"
                     /locus_tag="PKL26_05835"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNS83247.1"
                     /translation="MNSSFTTIGIRTPLIREGDEIASILVRAIRETPAREIQDGDILV
                     VAETAISTAEGRVVRLESVVPDRDAQRLAHRYGMDPALVQCILDESDEVVGGIPGFLL
                     CMKHGTLLPNAGVDASNAPPGCVTPLPADPDASALRIKEVIELLTGVRVGVIVADSRT
                     HAMRVGCSGVAIGCAGIPSVMDARGRTDLFGRPLMVTRLALADNLASAAEVVMGEADE
                     RTPAAVIRGLGIQVTDISGIESINAEECIFMGLLKQE"
BASE COUNT          470 a          699 c          740 g          459 t
ORIGIN      
        1 cacgacttgc acggttgaag gaagaggttg aagcatcggg ccttactccc gaactcgcca
       61 cagtaatcgt aggcaaagac ccggcatcgc agatgtatgt ccgcatgaag cacagggcct
      121 gcggacaggt gggcatcagg tcaagaggtg tcgaactccc ctcggactcc accaccgagg
      181 aggtacgcga gcaggtcgat aggctcaacc aggaccagga tgtcagcggg atcctggtgc
      241 agcttccgct ccccccgcat gtcgacactc atcgcgtggt gtagacagtc ctccccgaaa
      301 aagacgtaga cggtttccac cccgcaaacc tcgggcgtct cttctcaggg aacccgatct
      361 tctcgccctg cactccgagg gggatcatga cactcctcca ggaatacggg gtcgagccgg
      421 aagggctgcg tgccgtggtt attgggcgga gtatagaggt gggacgacca atggccgccc
      481 tccttctcaa tgccagcgcc acggtgacga tctgccacag ccggacccgc gaactccctt
      541 cattcacacg ccaggccgat atcctggtgg cggcagtggg aaaagcccgt tttgtggaac
      601 ccacgatggt cagggaggga gcggtggtca ttgatgtcgg cacgaaccag gtagacggaa
      661 aactgtgcgg ggatgtcgac tttgaccggg tcagggaaat cgccagtgcc atcaccccgg
      721 taccgggagg ggtaggcccg atgacgattg catccctcat ggagaacacc ttccttgcgg
      781 caaggctgca gtcatgcacc ggtgcacgat aggtgggctg actgttggtg acggatcccc
      841 cgtccgcctg atgggggtga tcaactgcag ccccgagtca ttctactcag agtcctatac
      901 cccggcgggg gatgtctttt cgaaggcctg tcagcttgtc gaacagggtg cagatatcat
      961 cgatgtgggt gcgaggagca ccgctcccgg cgccccgcca ctcacccgtg gagaggaggc
     1021 tgcaaggatg gtgcaggccc tctcggtgct tgagggatca ggcatccggg tctcggtgga
     1081 cacaatggac ccggcggtcc tcgaggcgtg cctcgaatac gatattgctg caataaacga
     1141 tatctcgggc ctcctcaacc ccgaatacgc ggcaggggtg tctggatctg gtctccctgc
     1201 actcctgatg gcatcgaaat gggcggtcgg cgacgcgtgc agcctggatg agacgcaccg
     1261 ggccctctcg atggtggaga ccagggcgca cgcatccggc gtgaccgagt acgtcctcga
     1321 tccggcaatc ggcctctggc aggccagccg cacggcagat cttgattggg aactctgccg
     1381 gcagttcagg cagttctctg tataccagcg cccactgctt gccgcagttt cgcgcaagtc
     1441 ctttcttgga atccttgtag gaaaaccacc tgcaggcagg ctcgcggcat caatcgccat
     1501 ggcctcgctt ctggtctgca tgggtgcaga tatcgtccga acacacgatg tcgccgagac
     1561 acgcgatgcc atcctcgtcg ccgaaaagat gaggcggtgg caatgaattc ttccttcacc
     1621 acaatcggca ttcgcacccc cctgataagg gaaggcgatg agatcgcttc gatactcgtc
     1681 cgggccataa gagagactcc tgcacgggag attcaggatg gtgatatcct ggttgtcgcg
     1741 gagaccgcaa tctccactgc agaaggacgt gttgtccgcc tcgaatcggt agtcccggac
     1801 agagatgccc agcgcctggc ccaccggtat gggatggatc ctgccctcgt ccagtgcatc
     1861 cttgacgaga gcgatgaagt agttggtgga attcccggat ttctcctctg catgaagcac
     1921 gggacgctcc ttccgaacgc aggtgttgat gcctcgaatg caccgccagg ctgtgtgaca
     1981 cccctgcccg cagatcccga tgccagcgca ttacgcataa aggaggtgat tgaactgctc
     2041 accggggtcc gcgtgggtgt gattgtcgcc gacagccgga cgcatgccat gcgcgtgggg
     2101 tgcagtgggg tcgctatcgg ctgcgcgggg ataccctctg taatggacgc acgcggccgg
     2161 actgaccttt tcggcaggcc gctcatggtc acccgcctgg cactcgcgga caaccttgcc
     2221 tccgctgccg aggtcgtgat gggagaggca gacgagcgca ctccggccgc cgttatccgg
     2281 ggtcttggga tccaggtgac tgacatttct ggtattgagt caattaatgc agaagagtgc
     2341 atcttcatgg ggctgctgaa gcaggaga
//
LOCUS       DAOQVJ010000250         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55396_length_2367_cov_4.413927, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000250 DAOQVJ010000000
VERSION     DAOQVJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55396_length_2367_cov_4.413927"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKL26_05840"
     CDS             complement(<1..680)
                     /locus_tag="PKL26_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS83248.1"
                     /translation="MSEYLIKNGFVFDPMQGIKGDRADIAIKDGKIVKSSDISSKAKT
                     IDASGKTVMAGAVEIHAHIAGPKVNEGRNYRPEDKLFSYTPKTATQRMAGGFSIPTTF
                     KTGYDYARMGYTTAMEAAMPPLFARHVHEEIKDTPIIDEGAYPVFGNNWFVMEYLKNQ
                     EIENTAAYISWLLEVTKGVAVKVVNPGGTEAWGWGLNCLTINDPVPYFDITPAEIVKG
                     LMEANEYLG"
     gene            complement(681..1997)
                     /locus_tag="PKL26_05845"
     CDS             complement(681..1997)
                     /locus_tag="PKL26_05845"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNS83249.1"
                     /translation="MSKSVTDVICPFCGTLCDDLEVVVSDDGRKIEDIFNACAIGAEK
                     FMHAQAAGRVTKPRMQQPDGSYKEVSYDEAVEYTAQMLHKSRKPLMYGWSSTSCEAQS
                     VGHEIAEKVGAIVDNTATVCHGTTLIAVQDVGIPSCTLGEIKNRADRIIFWGCNPCHA
                     HPRHQSRYSIFPRGYFTNKGHKNRKMIVVDPRYTDTAKMADYHFKIEQGRDYELLSAF
                     RVALRNEPLPEKVAGIPREKIIEAAEVLKSGRFGIIFFGMGVTQSLSKNHNIDNAIML
                     TKDLNEFTKFSIMAMRGHYNVTGSGQVLGWQFGFPYAVDLSRGFARYNPGESTSNDLL
                     RRGEVDAVFVLGSDPGAHFPISSVKKIAKLPSVCVDPHITPTSEISKLHVPVAFVGVE
                     VGGNCYRMDNVPIEARKVVDPPEGMLTDVEFLTRVNTRLEQLSGGK"
     gene            complement(1997..>2367)
                     /locus_tag="PKL26_05850"
     CDS             complement(1997..>2367)
                     /locus_tag="PKL26_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNS83250.1"
                     /translation="RSIQQGVAMEGGKEKDAYTKACGIIEMDPSDLKKLGAWRGTNVR
                     VTSKYGDVIVKAVEATQGPHPGLGYIPMGPWANSVVNPNTYSTGMPTFKGTPIEVEIA
                     MNEKVFSSVELVQKKCRGEA"
BASE COUNT          398 a          708 c          689 g          572 t
ORIGIN      
        1 ccgagatact cgttggcctc catgaggccc ttgacaatct ctgcgggggt gatgtcgaag
       61 taagggaccg ggtcgttgat cgtcaggcag ttcaagcccc atccccaggc ctctgtgccg
      121 cctgggttga cgaccttgac cgcgacaccc ttggtcacct cgaggagcca ggagatgtat
      181 gccgcggtgt tttctatctc ctggttcttc aggtactcca ttacgaacca gttgttccca
      241 aagacagggt acgcaccctc gtcgatgatg ggagtgtcct tgatctcctc gtggacatgc
      301 cgggcaaaga gcgggggcat tgccgcttcc attgcagtgg tgtaccccat ccgtgcgtaa
      361 tcatacccgg ttttgaaagt ggtcgggatc gagaacccgc cggccatccg ctgggtggcg
      421 gtcttaggag tgtagctgaa gagcttatcc tccgggcggt agtttcgtcc ctcgttcacc
      481 ttcggcccgg caatgtgtgc gtggatctcc accgcacccg ccatgaccgt ctttccgctg
      541 gcgtcgatgg tctttgcttt ggaggagatg tcgctgctct tgacgatttt tccgtctttt
      601 atggcgatgt cggcgcggtc tcccttgatc ccctgcatgg ggtcgaagac aaagccgttc
      661 ttgatcaggt actctgacat ctacttccct cccgagagct gttcgaggcg ggtgttcacc
      721 cgggtgagga actccacatc ggtgagcatg ccttccggcg ggtccaccac tttccttgcc
      781 tcgatcggca cattgtccat ccgatagcag ttcccgccga cttccacgcc gacgaatgca
      841 accgggacgt ggagcttcga gatctcgctc gtgggagtga tgtgggggtc cacgcatact
      901 gacgggagtt ttgcaatctt cttcaccgag ctgatcggga agtgtgcacc cgggtcgctc
      961 ccgagcacga acaccgcgtc aacctctcct cgcctgagga ggtcgtttga cgtggactcc
     1021 ccggggttgt accgtgcaaa gccacgggag aggtcgaccg cgtaggggaa cccgaactgc
     1081 caccccagga cctgcccgga gccggtgacg ttgtagtgtc cccgcattgc catgatgctg
     1141 aacttcgtga actcgtttaa gtccttcgtc agcatgatgg cattgtcgat gttgtggttc
     1201 ttcgagagcg actgcgtcac tcccatgccg aagaagatga tcccgaaacg cccgctcttc
     1261 agcacctcgg ctgcctcaat gatcttctct cttgggatcc cggcaacctt ctcggggagc
     1321 ggctcgtttc ttagcgccac acggaatgcc gagaggagct cgtagtcacg gccctgctcg
     1381 atcttgaagt ggtaatccgc catcttggca gtgtcggtgt accgcgggtc taccacgatc
     1441 atcttcctgt tcttgtgacc cttgttggtg aagtacccgc gtgggaatat cgagtagcgg
     1501 gactggtgcc gcgggtgtgc atggcagggg ttgcagcccc agaagatgat cctgtcggct
     1561 cggtttttga tctccccgag ggtgcagctg gggatcccca cgtcctgcac cgcgatcagg
     1621 gtggtgccat ggcagaccgt tgcggtgttg tcgacgatcg cccctacctt ctcggcgatc
     1681 tcgtgcccga cactttgtgc ctcgcagctg gttgaactcc agccgtacat cagcggtttt
     1741 cgggacttgt ggagcatctg ggcagtgtac tccactgctt catcgtagga tacttccttg
     1801 tagctgccgt caggctgctg catgcggggc tttgtcaccc ggccggcagc ctgggcgtgc
     1861 atgaacttct ctgccccgat ggcacaggcg ttgaaaatat cctcgatttt cctgccgtcg
     1921 tctgatacga ccacctccag gtcgtcgcac agggtcccgc agaacggaca gattacatca
     1981 gtgacggatt tggacatcag gcttcacccc tgcatttctt ctgaacgagc tcaacggagc
     2041 tgaacacctt ctcgttcatc gcaatctcaa cttcgatagg ggttccctta aaggtaggca
     2101 tcccggtaga gtacgtgttc gggttgacca ccgaattggc ccatggcccc atgggaatgt
     2161 agccgaggcc ggggtggggg ccctgcgtgg cctccaccgc cttgacgatc acatccccgt
     2221 acttgctggt gacacggaca tttgttcctc tccaggcgcc gagcttcttg aggtctgacg
     2281 ggtccatctc gatgatgccg caggctttgg tataggcgtc cttctccttt ccgccttcca
     2341 tggccactcc ctgctggatg gagcggc
//
LOCUS       DAOQVJ010000251         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55468_length_2365_cov_4.371429, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000251 DAOQVJ010000000
VERSION     DAOQVJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55468_length_2365_cov_4.371429"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..775
                     /gene="ribB"
                     /locus_tag="PKL26_05855"
     CDS             80..775
                     /gene="ribB"
                     /locus_tag="PKL26_05855"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNS83251.1"
                     /translation="MIGNALAELRDGKFVLLYDFDNRERETDFAIRSDAVTPRHIFRM
                     RKDGGGLICTAVHPVAARRLGLPFASDALRAMAVAEREGDIPYDRKNHSSFSLWVNHR
                     DTFTGITDRDRALTVNAIAEQVKCALNGGGAQFHEIFRTPGHMALLRAADGLLSVRRG
                     QTELSIAMAEMAGITPAVTICEMLDDESGYALSKEDAMAYAKKHGMVFVEGNEVLEAW
                     DAFVSGGKRGIPE"
     gene            complement(893..1054)
                     /locus_tag="PKL26_05860"
     CDS             complement(893..1054)
                     /locus_tag="PKL26_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83252.1"
                     /translation="MKDEEIQEMVKNLDLKTLKEAAKAAGIKPGRCPTKTSIAKMLPE
                     ETLKMLAKK"
     gene            complement(1126..1698)
                     /locus_tag="PKL26_05865"
     CDS             complement(1126..1698)
                     /locus_tag="PKL26_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HNS83253.1"
                     /translation="MPGRRDGVFEREPARRVFAQELRESRVHFREGQDEKSPTFVLLP
                     TGARCNRVFFAGTLTEKKKQGEQNMFYRARVVDPTGTFFVMAGSYQPEAMQQIARLEP
                     PCFVAVAGKPNVYQTPDGAVLISIRCESITQVDKDIRDQWVLDCARATLDRIEKSAGT
                     PDGDRARREYTIDPGLFRKMVYEALAQLKI"
     gene            complement(1719..>2365)
                     /locus_tag="PKL26_05870"
     CDS             complement(1719..>2365)
                     /locus_tag="PKL26_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNS83254.1"
                     /translation="ILQSGMFADETGAIRFVIWKEEGRERLNSAGIYTVYYATVDDFN
                     GRLSLTLNTATTVPEEGDIEVNTGNSLFEGAFVHLSPGSGLVRRCPVEGCNRVLSRQN
                     YCPVHEIQPNYHYDLRIKGWLDNGEETREVLIQRGETEGLVGMSLDQAKELAENNPLG
                     MDEVFLRFREALLGRYLSCRGREIDNRLLVSSCELLHYKPEQLADLLNRAGGSL"
BASE COUNT          428 a          752 c          711 g          474 t
ORIGIN      
        1 tcgaggtgat cgcgccggtc gcgctccgca ggaagctcgg tgttgaggac agcgacactg
       61 ttaccgtcga ggtgggggag tgatcggaaa cgcccttgcg gagctgcggg atggcaagtt
      121 cgtcctcctc tacgacttcg acaaccggga acgtgagacc gattttgcga tccgctcgga
      181 tgccgtcaca ccccgccaca tcttccggat gagaaaggat gggggaggcc tgatctgcac
      241 cgctgtccac ccggtcgcgg cccggcgcct cgggctcccc ttcgcaagcg acgccctccg
      301 cgcgatggca gtcgccgaga gggaaggcga tatcccctac gaccggaaga accactcctc
      361 gttctcgctc tgggtgaacc accgcgacac cttcaccggg atcaccgaca gggaccgtgc
      421 gctgactgtg aacgccatcg ccgagcaggt gaaatgcgcg ctgaacggcg gaggcgcaca
      481 gttccacgag atcttccgga caccgggcca catggcgctg ctcagggctg cagacgggct
      541 tctctccgtc cggaggggcc agaccgagct ctcgatcgcg atggcggaga tggcaggcat
      601 cacccccgcg gtcaccatct gcgagatgct ggacgacgag agcgggtacg ccctctcgaa
      661 ggaggacgcg atggcctacg caaaaaagca cggcatggtc ttcgtggagg gaaacgaggt
      721 gctcgaggcc tgggatgcgt tcgtctccgg agggaagcgc gggatcccgg agtaacagcc
      781 acgcgattcc tcttcacggg caagagacct ctcttttttc ccgcatgaat cctgaattgt
      841 cattgcagga aaagcgtcca cgcggttttt agagcaaaaa aaggtgcggg agttactttt
      901 tggccagcat cttcagagtc tcttcaggga gcatctttgc aatcgaggtc ttcgtggggc
      961 agcggcccgg cttgatacct gctgcctttg cggcctcttt tagtgtcttg aggtcgaggt
     1021 tcttcaccat ctcctggatc tcttcatcct tcataaggtc accagtaaga tctaccctcg
     1081 cgccctggag ataaaagttg ttgatcccgg gaggatcccg ggaaatcaga tcttcaactg
     1141 cgcgagcgcc tcgtacacca tcttccggaa tagccccggg tcaatggtat actcccttct
     1201 cgcccggtcc ccgtccgggg tcccggcaga cttctcgatc cggtcaaggg tcgccctggc
     1261 gcagtcgagc acccactggt cccggatgtc cttgtccacc tgggtgatgg actcgcagcg
     1321 gatggagatc agcacggccc cgtcaggggt ctggtacacg ttcggttttc ccgccacagc
     1381 cacgaagcac ggcggctcga gcctcgcgat ctgctgcatg gcctccggct ggtagctgcc
     1441 cgccatcacg aagaaggtcc cggtggggtc caccacccgc gcccggtaga acatgttctg
     1501 ctcaccctgc ttcttcttct cggtgagcgt gcccgcaaag aagacccggt tgcatcgcgc
     1561 cccggtgggg aggagcacga acgtcgggct cttctcatcc tggccttccc tgaaatgcac
     1621 ccggctctct cttaattcct gtgcaaagac acggcgggcg ggctcgcgtt cgaagacgcc
     1681 gtccctcctc ccgggcattc ccttcgcgtt cccgttggtc acagcgaacc acccgcccgg
     1741 ttcaggagat cggccagttg ctcaggcttg tagtggagga gctcgcagga tgagacaagg
     1801 agccggttgt cgatctcccg tcccctgcag gaaagatacc tcccgaggag agcctcgcgg
     1861 aaccggagga agacttcgtc catcccgagg ggattgttct ccgcgagctc ttttgcctgg
     1921 tcgaggctca ttcccaccag gccctcggtt tcacccctct gtatgaggac ttcccgcgtc
     1981 tcctccccgt tgtcgagcca gcccttgatc cggagatcgt agtggtaatt gggctggatc
     2041 tcgtgcaccg ggcagtaatt ctggcgggag aggacccggt tgcatccctc gacagggcag
     2101 cgcctgacca gcccggaacc aggtgaaagg tgcacgaacg ctccctcgaa caacgagttc
     2161 cctgtattga cctcgatgtc cccctcctcc gggaccgtgg ttgcggtatt cagggtgagc
     2221 gagaggcggc cgttgaagtc atccaccgtt gcgtaatata cggtgtagat gcccgccgag
     2281 ttcaggcgtt ccctgccttc ctccttccag attacgaacc gaatggcccc ggtctcgtct
     2341 gcgaacatgc cagactgcag gatcc
//
LOCUS       DAOQVJ010000252         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55481_length_2365_cov_3.345022, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000252 DAOQVJ010000000
VERSION     DAOQVJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55481_length_2365_cov_3.345022"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKL26_05875"
     CDS             <1..476
                     /locus_tag="PKL26_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS83255.1"
                     /translation="GKNAAIAPVSELAHTVGLVFQNPDHMFFANTVAEEIKFGITNLG
                     IPDPDKVLERVMEKTGLSHARDLYPRWLSRGERQRLAIACVLAMQPKVIVLDEPTTGL
                     DGRESREIMEILKHLQRDGRAIVMVTHSRQIAEECTDRIIAMEGGKIASDITRRR"
     gene            481..1269
                     /locus_tag="PKL26_05880"
     CDS             481..1269
                     /locus_tag="PKL26_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNS83256.1"
                     /translation="MSEILQYIHKDGLFHRMHPGTKIFLILVVGILTILTLDIPLLCL
                     ILLAVLLLALAGKVLREVLQQMALILAMSVVFILITLITMPEGEVLGYLIPGGFLPVT
                     TGALYAGVLLTLRFAILIISFQVFVITTQPRDLVNTLEKAGVPIDYNLMFLIALRFIP
                     TLQIEARRIHEAQLARGYAPGTGFVGRIRSVAPVMVPLVSNALSRATVLGLTIDMRGY
                     RTRARTPFREERMNLLDRSVIVILAIILVAFLAGTILPSFGYAV"
     gene            1505..1828
                     /locus_tag="PKL26_05885"
     CDS             1505..1828
                     /locus_tag="PKL26_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83257.1"
                     /translation="MKKKAICVLALLLAAILIVAPAAAGIYSPQKVSPPQFSSQRTFL
                     AGVDINNPASFQSFMTTVRGMELTPPPQPKIKLPKYMGETVTYQNLFSSGCAGCNCCG
                     CCGCC"
BASE COUNT          523 a          712 c          604 g          526 t
ORIGIN      
        1 acgggaagaa tgcggcgatc gcaccggtgt cagaacttgc ccacactgtc ggcctcgtct
       61 tccagaaccc agaccacatg ttctttgcca acaccgttgc cgaggagatc aaattcggca
      121 tcacaaacct cgggatcccc gaccctgaca aggtgctgga gagagtgatg gagaagaccg
      181 gcctctccca tgcccgcgat ctctatcccc ggtggctctc gcggggggag cggcagaggc
      241 ttgccatcgc atgcgtgctt gcaatgcagc cgaaggtgat cgtcctcgac gagcccacca
      301 ccgggctcga cggccgcgag tcccgcgaga tcatggagat cttaaagcac ctccagcggg
      361 acggccgggc tatcgtgatg gtcacccaca gccggcagat cgccgaggag tgcacggatc
      421 ggatcatcgc gatggaaggc gggaagatcg catcagacat caccaggagg aggtgagcga
      481 gtgtccgaaa tcctccagta cattcacaag gacgggctct tccaccggat gcatcccggc
      541 accaagatct ttttgatcct ggtcgtcggc attcttacca tcctcactct tgacattccc
      601 ctgctctgcc tgatcctcct cgcagtactt ctcctggcac tggcaggaaa ggtgctgcgc
      661 gaggtactcc agcagatggc gctcatcctg gcgatgagcg tggtattcat cctcatcacg
      721 ctgatcacca tgccggaagg cgaggtcctc gggtatctca taccgggagg cttcctcccg
      781 gttacgaccg gggcactgta cgcgggagtc ctcctcacgc tccggtttgc catcctcatc
      841 atctccttcc aggtcttcgt gatcaccacc cagccgcgtg accttgtgaa caccctcgag
      901 aaggcagggg ttcccatcga ttacaacctg atgttcttga tagcccttcg gttcatcccg
      961 acgctgcaga tcgaggcgag aaggatccac gaggcccagc tcgcgcgggg ctatgctccc
     1021 ggaaccggct ttgtcggcag gatcaggagc gtcgcacccg tcatggtccc gctggtctca
     1081 aacgccctct ctcgggcgac ggtgcttggc ctcaccatcg acatgcgggg ctaccgcacc
     1141 agggcccgga cacccttccg cgaggagcgg atgaacctcc tggaccgttc ggtcatcgtc
     1201 atcctcgcaa tcatcctggt cgcattcctt gccggcacca tcctcccgtc ttttgggtat
     1261 gccgtttgaa agacacaaca ttttttatcg cgctggccgc atctctctca catgccaacc
     1321 tgcagcgact gcttcctcta tacccctggg aaggggggaa aagaagggga gtgccggata
     1381 aatggaccgg cttcccctga ccgcgatgcg gaccggtgcc cctcgcggac cttcaggcca
     1441 aaagaataat gcgtggcact ctacccgggt aacattgaaa taatgcgatt gagtactctt
     1501 attcatgaag aagaaagcca tctgtgtact ggcactcctg ctcgcggcga ttcttatcgt
     1561 ggctcccgca gctgccggta tctattcccc ccaaaaagtg tctcccccac aattctcctc
     1621 gcagagaacc ttccttgccg gagtggacat caataacccc gcatctttcc agtcgttcat
     1681 gaccacggta agagggatgg aactcactcc acctccacaa ccaaagatta aactgcccaa
     1741 atacatgggt gaaaccgtga cctaccagaa ccttttcagc agcgggtgtg ccggctgtaa
     1801 ctgttgcggc tgctgcggtt gctgctgatc cttcgcatct cttttctgtt caattttttt
     1861 agctcttcgc tatctggtgt aggtaggatt ggtgaaccgg atacactcta ggagctgtcc
     1921 gcccccacac ccccccatac gcgatacacg ccgccgcccc caaaggacgc cccggctgcg
     1981 gttcagtgac tgacttggta tgcagataaa aacatcgatt cagtcttcct acacaaaaca
     2041 gcatgaaggc attttttttg acttgaattc ccgcaggacg cacttttgta tttcaatcct
     2101 tcgatgaacg cagagatgat tcattcaatc cccaaatata atctgagcga tcgttgtatt
     2161 gatacggggg attccggaga ccgaccggaa aatgcatggc atttgggtcg atgagaaaga
     2221 tcctaccatt tcacggtatg accgtaatat ctccagggca aatcgatatc ttcttaccaa
     2281 ataataaaaa ataaaagaga ggcatgtgaa gaatattttg aaggttaggc tgtaaatatt
     2341 ccactttcag ataatccgga gcagg
//
LOCUS       DAOQVJ010000253         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55822_length_2356_cov_6.272925, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000253 DAOQVJ010000000
VERSION     DAOQVJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55822_length_2356_cov_6.272925"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKL26_05890"
     CDS             complement(<1..659)
                     /locus_tag="PKL26_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83258.1"
                     /translation="MGLLGGLLDYYRGLGLESHIAQALFILGIFAVGGIIFIFFFALH
                     VPYEMSLVNMTVANATNATIPVETGTKILSSWDSAFSVISVFGVGVLVAGGSFFLGAL
                     LGFLFGIPKMISQPAGGDNTARTSQQYRANTNLEDISDWLTKIVIGVGLVELSAVPSL
                     LDMYAKMIAPALGGVPSSGAVAIAIFLYYSLVGFLIVYLATRGYMESDLEKQERMELY
                     R"
     gene            complement(1077..1904)
                     /locus_tag="PKL26_05895"
     CDS             complement(1077..1904)
                     /locus_tag="PKL26_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNS83259.1"
                     /translation="MHTLRSLLLFAILLISLLGSGCLDGLETKAPDHNSMLVILGDAT
                     GAIQGALDELERVASMTAASLEKTGIAGPDAEAVLKGRISSYPPVLTMITCNANGTVV
                     AAEPESVKVLVGQDLSGQEVVQRTLAEKVPVMSGMFPLAEGGNGVAVAHPVRSADGKF
                     LGVISTMFVPYDLIRPIAEDAANGTPYTFMVIETGGHILYDPDPGEVGKETFGDALYA
                     QFPEIDRVARHAAANRSGYDTYSFYGTGSDAVVRKETFWSTVSLHGTEWRVMVIGEG"
     gene            2103..>2356
                     /locus_tag="PKL26_05900"
     CDS             2103..>2356
                     /locus_tag="PKL26_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS83260.1"
                     /translation="MAKADKPEQPASSRQKYRRWILLLTFIAIPVTIFYISPIVIMMG
                     ASEGIVTGSFLLYIVLFVLSLFAGRIWCGWLCPMGACQELG"
BASE COUNT          512 a          683 c          641 g          520 t
ORIGIN      
        1 tctctgtata actccatcct ctcctgcttt tctaaatcgc tctccatata cccccttgtc
       61 gccaggtaca cgatgaggaa acctaccaga gaataataca ggaaaatcgc gatcgcaaca
      121 gcgccgctgc tcgggactcc ccctaaagcc ggtgcaatca tcttcgcata catgtccagg
      181 agtgaaggga ccgcactcag ttcgaccagt cccacgccaa tcacaatctt ggtcagccag
      241 tctgaaatgt cctcgaggtt cgtgtttgcc cggtattgct ggctggtcct tgcggtattg
      301 tctcctcccg cgggttgcga tatcatcttc gggattccaa acaggaaacc aagcagggcc
      361 cccaggaaga aggagccgcc tgcaaccagt acccccaccc cgaagacaga gattacggag
      421 aatgccgagt cccaggacga aaggattttc gtgcccgttt ccaccgggat ggtggcgttc
      481 gttgcattcg cgacagtcat attcacaaga gacatctcgt aggggacatg gagtgcgaag
      541 aagaatatga atataatgcc ccctaccgca aagataccga gaatgaagag tgcctgtgcg
      601 atatgactct caaggcccag gccacggtag taatccagca atccgccaag caaccccatc
      661 aagtcccccc gctgattttt ttattcgtaa tgaagaataa ctggtttggg tatataaggc
      721 ttgaccccca gaaggatccg gccttccctg gatcccctcc gtttacccgt gttgcatggg
      781 gtgcgtcaat gcaccccaat caaaaagcga ttcgaagcgg tccaggaaag atacggtatt
      841 ttcaggaaat acagtatacc ctgtggaggc ggttccctct gtcgcacctg gtaaaaggtc
      901 cgctaggtat ggtatttcgt gagtataact ggatagagag tcccatcgtc cgcataaatc
      961 attatggagg tgggcaaggt agcagtatag tgggcggagg gaatatcccg taacctcccc
     1021 ctgtttcggc gcaggatagg aattgctgta gcaccaagaa tgggggagaa tcctcctcac
     1081 ccctccccga tcaccatcac cctccactct gtcccgtgga gactgaccgt cgaccagaag
     1141 gtctctttcc ggacgaccgc atccgaccct gtgccgtaga acgagtaggt gtcgtatccc
     1201 gagcggttcg cggcagcgtg gcgtgcgacc cggtcgatct ccgggaactg tgcgtagagc
     1261 gcatccccga aggtctcttt ccccacttct cccgggtccg ggtcgtagag gatatggccc
     1321 cctgtctcga tcaccatgaa ggtgtacgga gtcccgttcg ccgcatcctc cgcgattggc
     1381 ctgatcagat cgtacggcac gaacattgtg ctgatcaccc caaggaactt tccgtccgcc
     1441 gacctgacag ggtgcgcgac tgccaccccg tttcctccct cggcaagcgg gaacatgccg
     1501 ctcatcaccg ggaccttttc cgcgagcgtc cgctgcacga cttcctggcc ggagaggtcc
     1561 tgccctacaa gcaccttcac gctctccggc tccgcggcga cgaccgtgcc gttcgcattg
     1621 caggtgatca tagtgaggac gggggggtag gatgagatcc tccccttcag cacggcctcc
     1681 gcatcaggcc cggcgatccc ggtcttttcg agcgacgcgg ctgtcatcga ggcgactctc
     1741 tcaagttcgt caagggcacc ctggatggcg ccggttgcat ctcctagaat tacgagcatg
     1801 ctattgtgat cgggggcctt cgtctccagt ccgtccaggc agccagaacc aagaagtgag
     1861 atgagcagga tggcgaagag taggagggaa cggagggtgt gcatgtgtga agtatgtatc
     1921 ggtggcaggg tggcttgtgg tttgcgatca gaagggttgt actttttacc agtaacatga
     1981 aggagaggtt cggggtatca ggggatcaga atcccccgct tccctctgaa ctcatcgccc
     2041 ggcacaggga gaccgcctga attattgtca tccgaatcat acctgaaatg caggaataaa
     2101 gtatggccaa agcagataag cccgaacagc ctgcatcatc ccgccagaaa taccggcggt
     2161 ggatcctttt gctcactttc atcgcaatcc cggtgacgat attctacatc tccccgattg
     2221 tcatcatgat gggagcatcc gagggtattg tgacgggctc cttcctgctc tacatcgtgc
     2281 tctttgtcct ctctcttttc gcagggcgga tctggtgcgg gtggctctgc ccgatgggtg
     2341 cctgccagga gctcgg
//
LOCUS       DAOQVJ010000254         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_55841_length_2356_cov_3.147327, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000254 DAOQVJ010000000
VERSION     DAOQVJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55841_length_2356_cov_3.147327"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKL26_05905"
     CDS             complement(<1..325)
                     /locus_tag="PKL26_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS83261.1"
                     /translation="MEGPGSLRQRLFRTKTIAELKEFCAGEHALKKAIGPIELVFFGI
                     GAVIGTGIFVITGIAAANYAGPALVISFVISGAACLFAALCYAEFATMVPVAGSAYTY
                     SFASLG"
     gene            750..>2356
                     /locus_tag="PKL26_05910"
     CDS             750..>2356
                     /locus_tag="PKL26_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNS83262.1"
                     /translation="MEQRIAGSIVEREVMAEMRDGTMLAADVYRPAWGEGPWPVLLIR
                     QPYDKAHAETFSYAHPSWYAAQGYIVVSQDIRGRWASGGEFSPFRHEAEDGYDTVEWA
                     AALPGSSGKVGMYGFSYGGLTQLMAATLAPPHLTCICPGFCTPQLFEGWAYNGGAFAL
                     SFNLTWALYLATDTARRKGLSGYEATLWKSIQSMHDWWQWLPMESFPLLRAHDLAPYF
                     FEWIDHPSNDAYWQRWSIERFYSRINVPALHIGGWYDIFRDGDLRLYEGLRAQAGSGK
                     ARENQKLVMGPWYHNPWNRVLGPCDLGDAAASRVDELQVQWFEYWLKGLGNGIMEEPR
                     VRYFMLGENRWRDDASWPPAKAEPVTLYLHSGGRANSLSGDGALTRELPGDEAPDVYV
                     YNPRDPVPSLGGQSCCIPGITPMGPADQRMVEAINQVLVYTMAPLETPFCIAGSVSVR
                     LFAATDVPDTDFCVKLVDVFPDGRAINLTSGILRAKFRDSLEHPHLLSPGIVDDFRID
                     GGSTAAQIRKGHRIRLEVTSSNFPCWDR"
BASE COUNT          471 a          701 c          729 g          455 t
ORIGIN      
        1 caccgagtga ggcgaagctg taggtgtagg cgctgcccgc cactgggacc attgtcgcga
       61 actcggcata acagagtgcg gcgaaaaggc acgcagcacc cgaaatgacg aatgagatga
      121 ccagcgccgg ccctgcatag ttcgccgcag cgattccggt gatcacaaaa attccggtcc
      181 cgatgacggc gcctatcccg aaaaagacaa gctcaatggg accaatcgcc tttttgaggg
      241 catgctcccc ggcacagaat tctttcaatt cagcgatagt ctttgtgcga aaaagccgct
      301 gccgcaggga gccggggcct tccatggtaa gagggatggc attgcggcct cttaaaacca
      361 agtatttttt ttagttgcct gcattattca aaaaggggca taagggtaag gaggcactgg
      421 atctttcccg gtatccggat acgaaccagg tgcccgccat tgcaagaaag attggagttc
      481 tgacaggaac caggatgtgc accttgcccc cacagggaat ttatgggaca tgaggatcag
      541 ggacctgcag gcgaccggca ctcatcgggg gtcgcaggcc tctttcccgg ctgaattcca
      601 gatgcaagaa agattttcca tcagtccgaa ccgggacgag attttctgtt cgtctcgtcc
      661 agccttatat cgtaaaccgg aatgaccgat tatccggaga agggcataaa tagtgccagt
      721 ccctgttttc catggaggaa cgccgaatca tggagcaaag aatcgcggga tccatcgtgg
      781 aacgggaggt catggccgag atgagagacg ggacgatgct cgcggccgac gtataccggc
      841 cggcttgggg agagggccca tggccggtcc ttcttatcag gcagccgtat gacaaggccc
      901 atgccgagac gttctcctac gcgcatccgt cgtggtacgc tgcccagggc tatatcgtcg
      961 tatcccagga catccgcggc cggtgggcct caggtggcga gttctcaccg ttccgccacg
     1021 aggcagagga cgggtacgat accgtggagt gggcggctgc gcttcctggt tcgtctggaa
     1081 aggtggggat gtacgggttc tcgtatggcg gcctgaccca gctgatggct gccactcttg
     1141 ctccccccca cctcacctgc atatgcccgg ggttctgcac tccgcagctc ttcgaggggt
     1201 gggcttacaa cggcggggcc tttgcgctct ccttcaacct gacctgggca ctatacctcg
     1261 cgaccgatac cgcccggcgt aagggacttt ccgggtacga ggccaccctc tggaaatcga
     1321 tccagtcgat gcacgactgg tggcagtggc tcccgatgga gtcgtttccc ctgctccgcg
     1381 cccacgacct cgcgccgtac ttcttcgagt ggatcgacca tccctcaaat gacgcctact
     1441 ggcagcgttg gagcatcgag cggttctatt cccggatcaa tgtccctgcc ctgcatatcg
     1501 gcgggtggta cgacatattc agggacgggg acctccggct ttacgagggg ctccgggcac
     1561 aggcagggtc agggaaggca agggagaacc agaaactggt gatgggcccc tggtaccaca
     1621 atccctggaa ccgggttctc ggcccctgcg accttggcga tgccgcggcc agccgcgtgg
     1681 acgagctgca ggtacagtgg ttcgagtact ggctcaaggg gctcgggaac ggcatcatgg
     1741 aagagccgcg ggtccgctat ttcatgcttg gcgagaacag atggagagat gacgcctcct
     1801 ggcccccggc aaaggcagaa cccgtgaccc tttacctcca cagcggtggg agggcgaact
     1861 ccctttcggg cgacggggcc ctcacccgcg agttgccggg ggacgaggcc cccgatgtct
     1921 acgtttacaa ccccagggac ccggtcccaa gtctcggagg gcagtcctgc tgcattcccg
     1981 gcatcacccc catggggcct gccgaccagc ggatggtgga ggcgatcaac caggtgctcg
     2041 tgtacaccat ggcccccctg gaaacgccct tctgcattgc ggggagcgta tccgtgcggc
     2101 tctttgctgc cacagatgtc ccagacaccg acttctgcgt gaagctggtg gatgtgttcc
     2161 cggatgggag ggcgatcaac ctcacatccg gcatcctccg tgcgaagttc cgggactcgc
     2221 tggagcatcc gcaccttctc tctccgggaa ttgtcgatga tttccgcatc gacggcggga
     2281 gcacggcggc gcagatccgg aaggggcacc gcatcaggct cgaggtgacg agcagcaact
     2341 tcccctgctg ggaccg
//
LOCUS       DAOQVJ010000255         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_56228_length_2345_cov_3.429694, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000255 DAOQVJ010000000
VERSION     DAOQVJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56228_length_2345_cov_3.429694"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..899)
                     /locus_tag="PKL26_05915"
     CDS             complement(27..899)
                     /locus_tag="PKL26_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83263.1"
                     /translation="MLRSDLFSILADVVRFHTPSGVELPLARHIAATYLSDRDCTIDA
                     MGNLLARPRGSTGRPLPLLNAHLDIHPRHSDPGDAGILARPGVISLVDGSDWVCRPSE
                     PVQVGFDNKIGVALALALVRRPGLDFKVVFSTQEETGRRGIQYAVETSPEFFEDTSFV
                     LTLDRHSSEGPDIIYQYKGRRMAPDGFLTWLERVSRQAGNPMYRRVGSPNRCADAYNI
                     SCAINGPVVNLSTGIYDEHTRYDRLNFREAEGTLRVVERCLREGWNPGIRPALGPAAG
                     ESGGMQEVLDAAGR"
     gene            complement(886..1851)
                     /locus_tag="PKL26_05920"
     CDS             complement(886..1851)
                     /locus_tag="PKL26_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83264.1"
                     /translation="MDQFIRKWFSAIRFGRPRNFGRMHIFPIYHDKTGGPEYLTLGEA
                     LKSGLLTITEVNESGSVPELKAVNRAELPILILDGEQLVGSKQDRVTNATILVPPMEA
                     ITIPVSCTEAGRWTYTSRAFTEAEFVMPAEIRKRKAGYIAESLKGGRGFEGDQGAVWR
                     DVGEMNEKAGAKSVSGAMKDGFEAKRHTIEEYRTAFKKMRRQKGVLVAIGGRVVALEM
                     VSSERAYRQLHPKIIRSYALDALLYEEQGSGEVNNATPWDFLGGPMASCTCEAFPSVG
                     LGKDVRVSGGEVTGSALVYQETPVHLSIFTGHRPEIRESYTHVEE"
     gene            2014..>2345
                     /locus_tag="PKL26_05925"
     CDS             2014..>2345
                     /locus_tag="PKL26_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83265.1"
                     /translation="MDKSGKNAHLFLAGPDIRYLEGSIRASYAYQMQSAVLLVRKTPE
                     TCNDSFLDEVRGLFGVGLDLRVEWITEEGVMEIAKRITDAALEQRRAGNTVLINICDN
                     DPFLVIPG"
BASE COUNT          466 a          718 c          647 g          514 t
ORIGIN      
        1 caaggatcag catcgccatc ggtccatcac ctccccgccg cgtcgagcac ttcctgcatc
       61 ccgccgctct cccccgccgc cggccccaga gcaggcctga tacccgggtt ccatccctcc
      121 cggaggcaac gctccacgac acggagcgtc ccctcggcct cgcggaagtt cagccgatcg
      181 tatcgcgtat gctcgtcata tatacctgtg gagaggttga cgacaggccc gtttatcgcg
      241 catgagatat tataggcatc cgcacaccgg ttggggcttc cgacccgccg gtacattgga
      301 tttccggcct gccgggaaac acgctctaac caggtgagga acccatccgg agccatccgg
      361 cggcctttat actggtatat gatgtccgga ccttcggatg agtggcggtc tagagtcagc
      421 acgaaggacg tgtcttcaaa gaactctggg gatgtctcga ctgcgtactg gatgcctctc
      481 cgcccggtct cctcctgcgt agaaaaaacg accttgaagt cgaggccggg gcgccgaaca
      541 agagcgagcg ccagggcaac cccgattttg ttgtcaaatc ctacttgcac cggctctgat
      601 ggccggcata cccagtcact cccatccacc aggctgatga cacccggtct ggccaggatc
      661 ccggcatctc ccgggtccga gtggcgggga tgtatgtcga gatgggcgtt gagcaggggg
      721 aggggcctgc cggtcgaccc tctcggccgc gcaagcaggt tgcccatcgc atcgatggtg
      781 cagtcccggt cagacaggta cgtggcggct atgtgcctgg caaggggcaa ctccacaccc
      841 gatggggtgt ggaagcggac gacgtcagcg aggatggaga agaggtcact cctcaacatg
      901 tgtataactc tccctgatct cgggacggtg cccggtgaag atcgagaggt gcaccggcgt
      961 ctcctggtac accagggccg atcctgtcac ctccccaccg ctcactcgta catccttacc
     1021 caggccgacc gagggaaaag cctcgcaggt gcatgaggcc ataggtccgc cgaggaaatc
     1081 ccatggtgtt gcattgttca cctctccact tccctgctcc tcgtagagga gagcatccag
     1141 agcgtagctc cggataatct tcgggtggag ctgccggtaa gcgcgctcgc ttgagaccat
     1201 ttcgagcgcg accacccggc cgccgatcgc gacgaggacc cctttctgcc gcctcatctt
     1261 cttgaacgcg gtgcgatact cctcgatcgt gtgccgtttc gcctcaaatc catccttcat
     1321 ggctcctgac accgatttag cccctgcctt ctcgttcatc tcaccgacgt cacgccagac
     1381 ggcaccctga tccccttcaa acccccggcc acccttcagt gattcggcga tatagccggc
     1441 tttcctcttg cggatctcag ccggcatgac gaactccgcc tcggtgaatg cccgggaggt
     1501 gtaggtccat ctccccgcct cggtgcagct caccgggatg gtgatcgcct ccatcggtgg
     1561 cacgaggatg gtggcgttgg tcacgcggtc ctgtttggaa cccaccagtt gctcaccatc
     1621 caggatgagg attggcagtt ccgccctgtt tacggctttc agttcaggaa ctgatccgga
     1681 ttcgttcacc tcggtgatcg tgagaagccc gctcttcagc gcctccccca gggtcaggta
     1741 ttccggcccc ccggttttgt cgtgatagat gggaaagatg tgcatcctgc cgaaattgcg
     1801 cggccttcca aacctgatgg ccgaaaacca cttgcgaatg aactgatcca ttgttctaac
     1861 tcctcagaca gagcttttac cagtttcgag cgctgtccct tgaccagtac atggccgcca
     1921 tagtttataa aaatatcgaa gtaactatat caaatgatta ctactattaa ccatgatcgg
     1981 tatcatatat ttatatcagt gcgatatata tgtatggaca aatccggtaa gaatgcccat
     2041 ttgttcctgg cagggcctga tatccggtac cttgagggtt cgatccgggc gtcatatgcc
     2101 taccagatgc aatcggccgt tcttctcgtg cggaagacgc ctgagacctg taatgactca
     2161 tttctcgatg aagtgcgtgg tctttttgga gtcggacttg atctccgcgt agaatggatt
     2221 accgaggagg gtgtcatgga gatcgcaaag aggatcaccg atgcggcgct ggagcaacgg
     2281 cgcgcgggca acacggtact gataaacatc tgcgataacg atcctttcct ggtcattccg
     2341 gggta
//
LOCUS       DAOQVJ010000256         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_56307_length_2343_cov_4.094406, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000256 DAOQVJ010000000
VERSION     DAOQVJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56307_length_2343_cov_4.094406"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(445..1044)
                     /locus_tag="PKL26_05930"
     CDS             complement(445..1044)
                     /locus_tag="PKL26_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNS83266.1"
                     /translation="MLEFTISADVYAWVILPLLIFLARICDVSLGTLRVIFIARGFKY
                     IAPAIGFFEVIIWLLAIGQVMQNVGNIASYIAYGGGFAAGTFIGMTVEERLSIGNVVV
                     RVITNRDAQGLVEAMREKNLGVTTMDAEGASGPVTVVLTISRREDLHLVFDLIHEFQP
                     NAFFTVEEIKSVKEGVFPRKHGTSLLNWRDPLGFFRKGK"
     gene            complement(1215..>2343)
                     /locus_tag="PKL26_05935"
     CDS             complement(1215..>2343)
                     /locus_tag="PKL26_05935"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNS83267.1"
                     /translation="SIPVRMDDGKIRAFQGFRVQYNDALGPTKGGIRFHPDETIDTIR
                     GLAALMTWKCALHNLPLGGAKGGIVCNPKELSEHELERLSRAYVQVMFPYLGPDRDIP
                     APDVYTNPAVMAWMMDEYSKIAGKTSFGSFTGKPVGLGGSEGRLDATARGGWYIVREA
                     ARDIGMNISGAGVAVQGFGNVGSHAALIGQEVFGARVVAVSDSRGGVYNPDGLAVHEL
                     VSHKAATGSVQHFPGATNITNEELIELPVEILVPAALENVISSRNAPQVRAGIVAEFA
                     NGPVDNDADEILFEKGTTVLPDFLTNGGGVIVSYFEMVQNFNMDQWDEGKVHRKLEKK
                     MTDAYREVHDLASQNNVPLRKAAYSIAVARVVEAMRKRGWV"
BASE COUNT          473 a          736 c          628 g          506 t
ORIGIN      
        1 tggaagactc gcgggtcttt tcagatgagg atgaacagtt tccggcgttt ccctcaggcc
       61 ggcagatccc tgacgacaca caggctgcag gatgaactga ttccgcacac aaatagggcg
      121 caagcattgg gcatccggac gccgaaaaac tccaggtttc ccggattttc cccccacaag
      181 tccttctttg ccttatctgc gccatggggg cagggtgtcc aaccggcatc aggttgagag
      241 gatcctcccc gtggtcaagg tgcaggatct gccggatctg gtcatctgag aacgccctca
      301 tggaaacctc accgggaatg ctgccctgaa tcccggcatc ctccctcgag aaccgttgcg
      361 gttctccatc aggattgtga gatctttggg tcggcacaac attccggact gatccaggga
      421 cagaggtcgg atgtccgctc acactcactt ccctttcctg aaaaaaccca gggggtcccg
      481 ccagttgagg agagaggtgc catgtttcct tggaaagact ccttccttca ccgattttat
      541 ctcctccacg gtgaagaagg catttggctg gaactcgtgt atgaggtcga agaccaggtg
      601 aaggtcctct ctcctgctaa tcgtgaggac cacggtgacc ggcccgctgg ccccctccgc
      661 atccatggtg gtaacgccga ggttcttttc tctcatggcc tccacaagac cctgtgcatc
      721 gcggttcgtg atgaccctga cgacgacgtt tccaatggag aggcgctcct cgactgtcat
      781 cccgatgaag gttcctgccg caaacccacc cccatacgcg atataggatg caatattccc
      841 gacgttctgc atcacctggc cgatagccag aagccagatg atcacctcga agaagccgat
      901 cgccggggcg atgtacttga acccacgggc gatgaatatc acccggagcg tgccgaggct
      961 cacgtcgcat atcctcgcca ggaagatgag caggggaagt attacccatg catacacatc
     1021 cgcagagata gtgaattcca gcacttagca atcacccccg gacgatttgt ctgttctgga
     1081 ctccctcatc atctcatcct gagagcggat gccagcccgt gccacaggcc ataccatgcc
     1141 gatgtaccgt gagatgccat tcctgataat tcccgcgtgg caatgactgg gggggaaggt
     1201 cgatgtacag gggattaaac ccagcccctc ttcctcatgg cctccactac cctcgcaacg
     1261 gcaatagagt acgccgcctt ccggagtggc acgttgttct gggacgccag gtcgtggacc
     1321 tcgcggtagg catccgtcat cttcttctcg agtttcctgt gcaccttccc ttcatcccac
     1381 tggtccatgt tgaagttctg gaccatctcg aagtacgaga cgatcacgcc accgccattc
     1441 gtgaggaaat ccgggagcac cgtggtaccc ttctcaaaga gtatctcatc ggcatcgttg
     1501 tccaccggcc cgtttgcaaa ctccgccacg attcctgcac ggacctgcgg ggcgttccgg
     1561 ctgctgatca cattctcgag cgccgccggg acgaggatct ccaccggaag ttcgatcagt
     1621 tcttcgttcg tgatgttcgt ggcacccggg aaatgctgca ccgacccggt cgcggctttg
     1681 tgggagacca gttcgtgcac ggcgagccca tctggattgt aaaccccgcc acggctgtca
     1741 ctcaccgcca ccaccctggc gccgaagacc tcctgcccta tgagtgcggc gtgtgagccc
     1801 acgttgccga acccctggac agcaacacct gcccccgaga tgttcatccc gatgtccctt
     1861 gcagcctcgc gcacgatgta ccatccaccc ctggcagtgg cgtcaagcct cccctccgac
     1921 ccgccaaggc caacgggttt gccggtgaag gagccaaatg atgtcttccc ggcaatcttc
     1981 gaatattcat ccatcatcca tgccattacc gctgggttgg tgtagacatc cggtgcaggg
     2041 atgtccctgt caggcccaag atacgggaac atcacctgca cataggcacg gctgagtcgc
     2101 tcgagctcgt gttccgagag ctctttcggg ttgcagacga tccctccttt tgccccgccg
     2161 agagggaggt tgtgaagggc gcacttccag gtcatcaggg cggcaagacc ccggatggtg
     2221 tcgatagtct catccgggtg gaacctgatt ccgccttttg taggaccgag tgcatcgttg
     2281 tactggacac ggaacccctg gaatgcccgg atcttcccgt cgtccatcct aacggggatt
     2341 gat
//
LOCUS       DAOQVJ010000257         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_56411_length_2340_cov_4.185558, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000257 DAOQVJ010000000
VERSION     DAOQVJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56411_length_2340_cov_4.185558"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKL26_05940"
     CDS             complement(<1..212)
                     /locus_tag="PKL26_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284076.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNS83268.1"
                     /translation="MRDVAVIGVGCTTFGEKWNTSFRDLFVEAGALALEDAGVSGEQI
                     GAMYVGNMSAGRFIEQEHIGALIADYA"
     gene            complement(209..1261)
                     /locus_tag="PKL26_05945"
     CDS             complement(209..1261)
                     /locus_tag="PKL26_05945"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNS83269.1"
                     /translation="MVGIITYGVYIPKFRIKVEEIARVWGANPDDISGGLGVYSKSVP
                     DLDEDTATIAVEAARNAMRRRAIDPQEIGAVYVGSESHPYAVKPTACTVGEAIGATPT
                     MTAADYEFACKAGTAGIQTCMGLVASGMVKYGLAIGSDVAQGAPSDALEYTAAAGGAA
                     FLIGNRDPIAEIHRTCSFTTDTPDFWRREGQDYPRHGGRFTGDPGYFRHIEGAVRLLF
                     EQTAKGPKDYTYAVFHQPNAKFPQRVAKILGFTPEQIRPGLVVPRLGNTYSGSSMIGL
                     AATLDCAKAGDRIFVASFGSGAGSDAFDIEVTDAIESGEFRRDEAPGVEKLLENPIFV
                     TYAQYARHKGKIVMQK"
     gene            complement(1255..1965)
                     /locus_tag="PKL26_05950"
     CDS             complement(1255..1965)
                     /locus_tag="PKL26_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS83270.1"
                     /translation="MKSDLRNRLAEKMAGEITLSDSPGKALKKWRMSFNIPQAVLSEH
                     LDVSPSVISDYESGRRKSPGTAVVGKIVDTILSIDEASGGKYIKKFAKILYSDVDDDV
                     IYDIHDYPSPVPLARFAELIQCQTVCGSLSQSIYGYTVVNSLNAITQLSSNEFNRIYG
                     WSTERALIFTDVTSGKSPMVAIRVTPFKPRVVVLQGIEPALVHPLVPKMAEKDRITVL
                     CTEMDVDRVVHILRDEQW"
     gene            2090..>2340
                     /locus_tag="PKL26_05955"
     CDS             2090..>2340
                     /locus_tag="PKL26_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNS83271.1"
                     /translation="MILPEELQCLKAIALLGGCRGSVRVSSQSLGTTLGTSPQTASRR
                     LQSLERQRLVSRTIAPDGQDIMVTPLGEGELRREYQEYSR"
BASE COUNT          412 a          748 c          689 g          491 t
ORIGIN      
        1 gcgtagtccg cgatcagggc gccgatatgc tcctgctcga tgaaccggcc cgcgctcatg
       61 ttgccgacgt acatggcccc tatctgctcg ccggagaccc ccgcgtcttc gagcgcgaga
      121 gcacctgctt ccacgaagag gtcccggaac gacgtgttcc acttctcccc gaaggtggtg
      181 catcccaccc ctatcactgc aacgtctctc atttctgcat cacgatcttg cccttgtgtc
      241 tcgcatactg tgcataggtc acgaagatcg ggttctccag gagcttctcg acccccgggg
      301 cctcatcgcg gcggaactcg ccggactcga tcgcatcggt gacctcgata tcgaaggcat
      361 cgctccccgc acctgaccca aacgacgcaa caaagatacg gtcgccggcc ttcgcgcagt
      421 cgagcgtcgc cgcaagcccg atcatggacg atccgctgta ggtgttcccg aggcgcggca
      481 ccaccaggcc gggcctgatc tgctctgggg tgaacccgag gatctttgcg acccgctggg
      541 gaaacttcgc gttcggctgg tggaagaccg cgtaggtgta gtctttcggc cccttcgcgg
      601 tctgctcgaa gagcagacgg accgcaccct cgatatggcg gaagtagccg gggtcgccgg
      661 tgaacctgcc gccgtggcgc ggatagtcct gcccctccct cctccagaag tcgggggtgt
      721 ctgtggtgaa cgagcaggtg cgatggatct ccgcgatggg atcccggttg ccgatcagga
      781 acgcggctcc cccggcggct gccgtgtact cgagggcgtc gctcggtgcg ccctgcgcga
      841 catcagaccc gatggcaagg ccgtacttca ccatgccgct tgccacgagg cccatgcagg
      901 tctggatgcc cgcggtcccc gccttgcagg cgaactcgta gtccgccgca gtcatcgtgg
      961 gggtggcccc gattgcctcc ccgaccgtgc aggcggtggg cttgaccgcg taggggtggg
     1021 actcgcttcc cacgtagacc gcgccgatct cctgagggtc gatcgccctc ctccgcattg
     1081 cattccttgc tgcttccacc gcgatggtcg cggtgtcctc gtcgagatcg gggaccgatt
     1141 tcgaatagac tccaagcccc ccgctgatat cgtccgggtt cgccccccag accctggcga
     1201 tctcttctac cttgatgcga aattttggta tatacacacc gtatgtgatg atgcctacca
     1261 ttgctcgtcc ctcagtatgt gcacgaccct gtccacgtcc atctcggtgc agagcaccgt
     1321 gatcctgtcc ttctccgcca tcttcggcac cagcgggtgc acgagcgcgg gttcgatccc
     1381 ctgcagcacc accactcggg gcttgaaggg ggtgacccgt atcgccacca ttggggactt
     1441 gcccgaagtg acatcggtga atatcagcgc cctctccgtg ctccacccgt agatgcggtt
     1501 gaactcgttc gaggagagct gggtgatggc attgaggctg ttgaccaccg tgtatccata
     1561 gatggactgc gagagtgacc cgcagaccgt ctggcactgg atgagttccg caaaccgggc
     1621 cagcgggacc ggcgacgggt aatcgtggat atcgtagatg acgtcgtcat ccacgtctga
     1681 atagaggatc tttgcaaatt tcttgatgta cttgcccccg ctcgcctcgt cgatggagag
     1741 gattgtatcg acgatctttc ccaccacggc cgtgccgggg ctctttcgcc ttccgctctc
     1801 gtagtcgctg atcacagaag gggatacgtc caggtgctcg gagagcactg cctgggggat
     1861 gttgaaactc atcctccact tcttcagtgc cttgcccggc gagtctgaaa gcgtaatctc
     1921 tccggccatc ttttcggcca gccgattccg caaatcagat ttcatggctc attatgcggg
     1981 ttcccatttt atataattaa cgaatgggga ttcgacattt cacgaaaaac cggtgcccgg
     2041 ttgcccgggt tggatatcca taaatagtaa gcatctcaat tctgatataa tgatccttcc
     2101 agaggagctc cagtgcctca aggcgatcgc gctcctgggg ggctgcaggg gatcggtcag
     2161 ggtctcgtcg cagtcgcttg ggacaacgct tggcaccagc ccccagaccg cgtcgcgccg
     2221 gcttcagtcc ctcgagcgcc agaggctggt gagcaggaca attgcccctg acggccagga
     2281 catcatggtc actcccctcg gcgaggggga gctcaggaga gagtaccagg agtacagccg
//
LOCUS       DAOQVJ010000258         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_56707_length_2332_cov_4.200264, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000258 DAOQVJ010000000
VERSION     DAOQVJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56707_length_2332_cov_4.200264"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKL26_05960"
     CDS             <1..203
                     /locus_tag="PKL26_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS83272.1"
                     /translation="GLIKRLKDAYRGESLDLTDGIRINREDSWALVRPSGTEPLVRVM
                     VESTSKTGARQFHEELMAHIRQ"
     gene            539..823
                     /locus_tag="PKL26_05965"
     CDS             539..823
                     /locus_tag="PKL26_05965"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNS83273.1"
                     /translation="MKTVEIRISGRVQKVGMRNCVRHIAGKLSIRGDVMNQADGTVRI
                     RATGDPIILDKFVSMLYSCPRALIRDIEILDIQFAQFPDFAVVRTEEETR"
     gene            complement(833..1777)
                     /gene="cas1"
                     /locus_tag="PKL26_05970"
     CDS             complement(833..1777)
                     /gene="cas1"
                     /locus_tag="PKL26_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNS83274.1"
                     /translation="MEWSVIEPVPWYLVSGFGAHLKATPRTLTVQKNGHTEELPLSRV
                     RHLLVMGGHHIHTSVVARLLASGSCISFFEADGEPLGIAKPFGFRYDEVVREAQAKAY
                     SHSFALAVAKGSIHSRIQALVGLQEARDEPLFYEGELEILEKSYAELEFLVRVEELRR
                     VHRLASDMYYEILARTIPQETGFRRRTARPHVDVVNTMLSFGYGLLFGNACVAAIGAH
                     LDPDLGFLHKGKGGLVYDIIDPFKPAMVDGMVSAIAREGIPEEEYECGPSRCILSDDL
                     LVVLTKALHETIRQEVIDEQVMALQGALIKKNDYLVIT"
     gene            2075..>2332
                     /locus_tag="PKL26_05975"
     CDS             2075..>2332
                     /locus_tag="PKL26_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNS83275.1"
                     /translation="MFWDEKTETLPKRDLEELQLTRLKKTIAQVQNVEFYRKKLKEAG
                     VRPDDIRTLGDIEKIPFTRKQDLRDGYPFGFFAVPLQQVVRI"
BASE COUNT          554 a          637 c          650 g          491 t
ORIGIN      
        1 cggggcttat caagcgcctg aaggatgcat acagaggaga gtcgcttgac ctcaccgacg
       61 ggatccgcat caaccgtgaa gactcatggg cacttgtccg gccgtcggga acggagccgc
      121 ttgtgcgggt gatggttgag tcgacctcaa agacaggggc gaggcagttc cacgaagagc
      181 tgatggccca tatcaggcag taaaccactg ccaggcatcc ctttttctca tcttttttac
      241 gatgcatggt ttcctcggcc gatctccccg gcaatggtcg cgaaccgggc atctcgccga
      301 acatgggcca ccactcttat acgagttcaa caactcataa aaatgccgta tttttgagaa
      361 aaagacgttt ttttaatcac tttttatttc atggcatgta cctttataca cctcatgatg
      421 cgatatcata acaggcgaac gctggaacgg gaatactgat atgccacgcc gttgacagat
      481 ccgatcgtct gaccacaaaa gggtgaatga agagagaccg ctttgaatgt ggggcatcat
      541 gaagacagtg gagatacgca tctcaggcag ggtccagaag gtggggatgc gaaactgcgt
      601 ccggcatatc gccgggaaac tcagcatccg gggagacgtg atgaaccagg cggacggaac
      661 cgtcaggatc cgggccaccg gggaccccat catccttgat aagttcgtct ccatgctgta
      721 cagctgcccg cgggccctca tcagggacat cgagatcctc gatatccagt ttgcccagtt
      781 tccggacttt gcagtagtca ggaccgaaga agagacccgt taggataccg tctcaagtga
      841 tgacgaggta atcgttcttt ttgatcaatg caccctgaag tgccatcacc tgctcgtcga
      901 tgacttcctg gcggattgtt tcgtggagag ccttcgtgag caccacgagc agatcgtcgg
      961 agagaatgca gcgggaagga ccgcactcgt attcctcctc ggggattccc tccctcgcaa
     1021 tggccgagac catcccgtcg accatcgcgg gcttgaacgg gtcgattata tcatagacga
     1081 ggccgccctt tcccttgtga aggaacccta agtcagggtc aaggtgtgcg ccgatcgcgg
     1141 ccacacaggc atttccaaag agaaggccgt agccgaagga gagcatggta ttcacgacat
     1201 ccacatgggg ccgggcagtc cgccgcctga accctgtctc ctgaggaatg gtgcgggcaa
     1261 ggatctcgta atacatatcc gatgcgaggc ggtgaaccct gcgcaactcc tccactctga
     1321 cgaggaactc gagctcggcg taactcttct ccaggatctc gagctctccc tcgtaaaaga
     1381 gtggttcgtc gcgtgcctcc tggagcccga caagcgcctg gatccgtgaa tggatactgc
     1441 ctttcgctac tgcaagggca aacgaatggg agtatgcctt tgcctgcgct tccctgacca
     1501 cttcgtcata acggaaacca aatggttttg cgattcccag cggttctcca tctgcttcga
     1561 agaaagatat gcaggacccc gatgcgagca gccgtgcaac cacggaggta tgaatgtgat
     1621 ggccgcccat caccaggaga tgccggaccc tggagagtgg caactcctca gtatgcccgt
     1681 tcttctgcac ggtcagggtt cggggtgttg ccttcaggtg ggccccgaat ccggatacca
     1741 ggtaccaggg gaccggctcg atgacgctcc actccatatt tttcttaaac gagggaaagg
     1801 tttccgatat actcatttcc cggattcttg tacttcaatg catcgttatc ccgaagaatc
     1861 ctccttttat tggcaggata cgcggacaag cgacgggagc catgctatga cttgacaatg
     1921 tttttcatcc gccggcacaa gattctatga gaagccgcgt tggatctcgc acgtgcaaca
     1981 attccctgca cggaaagcag acgaaaaacg ggctgaaccg gtggcagaat gacgagaatg
     2041 acaggagccc gagaggacct ggggacaagg gaccatgttc tgggatgaga agaccgagac
     2101 gctcccaaaa agagacctcg aggagttaca actcacgcgt ctcaagaaga ccatcgcgca
     2161 ggtccagaac gtggagttct atcggaagaa actgaaggaa gccggagtgc ggccggatga
     2221 catccggact ctcggagaca tcgagaagat cccttttacg aggaagcagg acctccgcga
     2281 cggctacccg ttcgggttct ttgccgttcc cctgcagcag gtggtccgca tt
//
LOCUS       DAOQVJ010000259         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57065_length_2323_cov_3.787919, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000259 DAOQVJ010000000
VERSION     DAOQVJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57065_length_2323_cov_3.787919"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKL26_05980"
     CDS             complement(<1..395)
                     /locus_tag="PKL26_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNS83276.1"
                     /translation="MNLRRQHALSIAYTGAFTAFLAAGSWISIPFVPVPFTLQTLFLF
                     LAAGLMGRYSVAPVSLYLLMGAMNFPVFHSGTSGIGVFLGPTGGYLLGFIPAALLSGL
                     GYERKLRLARYAGMAAGLCALYACGVAWLS"
     gene            complement(435..1412)
                     /locus_tag="PKL26_05985"
     CDS             complement(435..1412)
                     /locus_tag="PKL26_05985"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNS83277.1"
                     /translation="MYEAAFRVLEVLETADGPVSGETISNTLGISRSAVWKHIKELNN
                     MGYEITASHREGYRLKKSSNRLLPYEVHKKLRTAFMGKQMRYFESTTSTNWVGRQMAE
                     EKEPSSLHGMVIIAEQQTGGIGRLGRSWVSPRGGIWATVVIRPKIPIERVFMITMAGA
                     IAIARALRKEYDLGALIKWPNDIYIGDSKVAGLLLELSAEADQIHYALLGLGVDVNVS
                     PADLSGLPHAVTSVSLEVGHDVDRPALLARILREFESRYLLLEAGEYESIVREWKSLS
                     CTLEHRVRVHTLKTSIEGEAIDIDEYGALLVRKDNGKVERVIAGDCTQQ"
     gene            complement(1417..1839)
                     /locus_tag="PKL26_05990"
     CDS             complement(1417..1839)
                     /locus_tag="PKL26_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HNS83278.1"
                     /translation="MNRFILSAESGPEDLEPLGLALHELLNRLPITARSLERPGIRIE
                     DGRVIDANYSGPVLEQVLQENRILKVTPSRGAYKGVPVVVGPIRDSAGAAITAIGIVD
                     ITGIFDLATLMDHQSEILKQVCGKDPCPLPGEQVVAKR"
     gene            complement(1874..>2323)
                     /locus_tag="PKL26_05995"
     CDS             complement(1874..>2323)
                     /locus_tag="PKL26_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83279.1"
                     /translation="YSTLIIPAGFANPSYSRLLPALRASSGRIRRFLEAGGKIIVFGA
                     GIDRADAYDWLPFDVTYCHQHRDGGLECVSTHRCASLFAGYDPESIESDGYFSDHHGE
                     VIAKMGGSAVLIRSDVGDGEVIATTIHEYPSRAFLQEFCKGSLETFL"
BASE COUNT          476 a          696 c          667 g          484 t
ORIGIN      
        1 gagagccatg ccactccaca ggcgtagagc gcacagaggc ctgccgccat ccccgcgtac
       61 cgggcaagac gcaatttccg ctcatacccg agacccgaga gaagggccgc gggaatgaac
      121 cccaacaggt acccccctgt cggcccgagg aacaccccta tccctgacgt cccgctgtga
      181 aataccggga aattcatggc ccccatcagg aggtaaagag agaccggggc caccgagtat
      241 ctccccatga gccctgccgc gaggaagagg aagagggtct ggagggtgaa tgggaccggg
      301 acaaagggga tggagatcca tgaacctgcc gcaaggaatg cagtgaacgc tcccgtatag
      361 gcgatagaga gtgcatgctg ccttcggagg ttcatcgtca acctgagatt aattcgtggt
      421 tgacgagaag atagttactg ctgagtgcag tcgccggcga tcacgcgctc gacttttccg
      481 ttgtccttgc ggaccaggag tgcgccatac tcgtcgatat cgatcgcctc gccctcgatg
      541 ctggtcttga gagtgtgaac gcggacgcgg tgctccagcg tgcaggagag actcttccat
      601 tccctgacga tcgactcgta ttcgcccgcc tccaggagca ggtagcggct ctcgaactcc
      661 cttaaaatac gggcaaggag tgcggggcgg tccacgtcat gtcctacttc aaggctgacc
      721 gatgtcaccg cgtggggcag cccggagagg tccgcgggcg atacattcac gtccaccccg
      781 aggccaagca gtgcgtaatg gatctggtcc gcctctgcgg agagctcgag gaggaggcct
      841 gccaccttgc tgtccccgat atagatatcg tttggccact tgatgagggc cccgaggtcg
      901 tactcctttc gcagcgccct ggcgatcgca atcgctcccg ccatggtgat catgaagacc
      961 ctctcgatgg gaatctttgg cctgatcacg acagtggccc agatcccgcc gcgtggggag
     1021 acccatgacc tccccagtct cccgatgccc ccggtctgct gctcagcgat gatcaccatc
     1081 ccgtgcagcg acgatggttc cttctcttcc gccatctgcc tgcctaccca gttggtcgaa
     1141 gtggtgctct cgaagtagcg catctgcttg cccatgaagg cggtccggag tttcttgtgg
     1201 acctcgtagg ggagcagacg gttgctgctc ttcttgagcc ggtacccttc ccggtgggac
     1261 gcggtgatct cgtagcccat gttgttcagc tccttgatgt gcttccacac cgcagagcgt
     1321 gatatcccca gggtattgct gattgtttcg ccggaaacag gtccatccgc tgtctccagg
     1381 acttcaagga ccctgaacgc tgcctcgtac atgatgctac ctttttgcca cgacctgctc
     1441 tccaggaagc gggcaaggat ccttcccgca gacctgcttg agaatttcag actggtgatc
     1501 catgagggta gcgagatcga atatacccgt gatatccacg atccctatgg ctgtaatggc
     1561 agctcccgcc gaatcccgga tgggaccgac cacgaccggg acacccttgt aggctcccct
     1621 ggaaggggtt acctttaata tgcggttctc ctggaggacc tgttcgagga caggccctga
     1681 ataattcgca tcgatcaccc gcccatcctc gatcctgatg cctggccgct cgagggaacg
     1741 cgctgtaatg ggaagcctgt tcaggagttc atggagtgcc agtcccagtg gttcgagatc
     1801 ctccggcccg gattctgctg agaggataaa gcggttcata cgtggatcac tacactaaat
     1861 ccctgatttc cagtcacaag aatgtttcca atgaaccctt gcagaattcc tggagaaatg
     1921 cccgtgaagg atattcatgg atggtggtgg caattacctc gccatcgccc acgtcgctcc
     1981 tgatcaggac tgcactccct cccatttttg cgattacctc accgtgatgg tctgaaaaat
     2041 acccgtcact ctctatggat tccgggtcgt atcctgcaaa gagcgatgcg cagcggtgcg
     2101 ttgagacgca ttcgaggccg ccgtcccggt gctggtggca gtaggtgacg tcgaacggca
     2161 gccagtcgta ggcatccgcc cggtcgatcc ccgcaccaaa gacaatgatc ttccctcctg
     2221 cttccaaaaa cctccttatg cggcccgaag aggcacgcaa cgctggcagg agccgggaat
     2281 aggaggggtt tgcaaagcct gcggggatga tcagggtgga ata
//
LOCUS       DAOQVJ010000260         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57073_length_2323_cov_3.203263, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000260 DAOQVJ010000000
VERSION     DAOQVJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57073_length_2323_cov_3.203263"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="PKL26_06000"
     CDS             <1..1430
                     /locus_tag="PKL26_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83280.1"
                     /translation="AEIHDLGEMGIHLSLYQVTLKEIKNFPDDVVVRTIFTTGDIANC
                     VVISRRVFECPYPEIAPPKQSVSALRRQLQEMTAGIESARRELLLCARYRNSLSAIRI
                     GLEREIEFHQVLSGMGREGEINYVTGYIPYDAEEQLIEAARDKKWGVMITEPSDIDEV
                     PTLLRNPKWVSLIKPVLDLLGLTPGYRELDISLLFLVFFSIFFGILIGDAGYGVVYIL
                     ITLVLQKVVLKSWVLDHPEMKTTFALFYLLGACLVIWGLLTGVFFGQSWLLELGYTPV
                     VPQLNDPQFMQTFCFFLGALHLSIAHLWRVILKFPSLRLLADIGWICVLWTAFFLART
                     LILGDPFPLLGIWLIIAGIVLVILFTHPQRNVLKGIATGLGTIALSLVNNFTDVISYI
                     RLFAVGLATLAIAETTNSLAFGFGDGIVALVVGVVILITGHGLNIILGPMSVLVHGVR
                     LNVLEFSGHANVIWSGTAYEPFKEE"
     gene            1446..1901
                     /locus_tag="PKL26_06005"
     CDS             1446..1901
                     /locus_tag="PKL26_06005"
                     /inference="COORDINATES: protein motif:HMM:NF005174.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83281.1"
                     /translation="MDANLLTQFQDLGMAMVLALSACGSAAGTGTAGMAAIGAWKKNY
                     SQNKPAAFMLVAFVGAPLTQTIYGMIVMNQMSQLAMQGVFLWGIGGFCGAAMGLSAYF
                     QGKCGACACDSMGETGKGFGNYMIVLGMVETVALLVMAFTLGILGSFGT"
     gene            complement(2141..>2323)
                     /locus_tag="PKL26_06010"
     CDS             complement(2141..>2323)
                     /locus_tag="PKL26_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS83282.1"
                     /translation="VRLGNPAFEITKVAEEEDVSVIWMSSVGRGWFAELLIGSTAGAV
                     AVNSRRPVIIVRSKDT"
BASE COUNT          512 a          611 c          624 g          576 t
ORIGIN      
        1 ccgcagagat ccatgacctc ggggagatgg ggatccatct ctccctttat caggtaacgc
       61 tcaaggagat aaagaatttc ccggatgacg tggtggtcag gaccattttt accacgggcg
      121 acatcgccaa ctgtgtcgtg atatcgagga gggtgtttga atgcccgtac cccgagattg
      181 ctcccccaaa gcagagcgta tctgcgttga gacggcaact gcaggagatg actgccggaa
      241 ttgaatcggc caggagagaa ctccttctct gcgcccggta tcgtaactcg ctctctgcaa
      301 tcaggattgg ccttgagagg gagatcgagt tccaccaggt actgagcgga atgggccggg
      361 agggagagat caattacgtt accgggtata tcccgtatga cgctgaagag cagctgatcg
      421 aggcagcgag agataagaaa tggggcgtga tgatcaccga gccttccgat atcgacgagg
      481 tcccgacact tctcaggaac cctaaatggg tcagcctgat taagcccgtg ctcgaccttc
      541 tcgggctcac gcccggatac cgggaacttg acatcagcct cctcttcctt gtcttcttca
      601 gcatattctt cggcattctc atcggtgacg cgggatacgg ggtggtgtat atcctcatca
      661 ccctggtgct gcagaaggtt gtgctgaaga gctgggttct cgatcaccct gaaatgaaaa
      721 cgacatttgc gctgttttac cttcttggtg catgcctggt gatatggggc cttctcaccg
      781 gagtcttctt tgggcagtcc tggctgctgg aactcggata cacgcctgta gtgccgcagt
      841 tgaatgatcc ccaattcatg cagaccttct gcttcttcct cggcgccctc cacctctcaa
      901 ttgcgcattt gtggagagtg atactgaaat ttccttcgct tcggttgctc gcagacatcg
      961 gctggatatg cgtgctctgg actgcattct tccttgcccg gacacttatc ctgggtgatc
     1021 cattccctct gcttggcata tggctgatca tcgcaggaat tgtcctcgtc atcctcttca
     1081 cccatcccca gcgaaatgtc ctgaaaggta tcgccacggg actcgggaca atcgcgctga
     1141 gccttgtgaa caacttcacc gacgtcatct cctacatacg tctctttgca gtcggcctcg
     1201 cgaccctggc tattgcagaa accacgaatt cccttgcatt tggattcgga gacggcatcg
     1261 tggcacttgt cgtgggcgtc gtaattctta ttacaggaca tggattaaac atcattttag
     1321 gaccgatgtc cgttctcgtg catggcgtca ggctgaatgt tcttgaattt tccggacatg
     1381 caaacgtgat atggagcgga acggcatatg aaccattcaa ggaggagtaa caccgggaga
     1441 ttaccatgga cgcgaactta ctgacacaat ttcaggacct tggtatggcc atggtccttg
     1501 cactttccgc ctgcgggtct gccgcgggga cgggaacggc aggaatggcc gcgatagggg
     1561 catggaaaaa gaactacagc cagaacaaac ccgcagcatt catgctcgtt gcgtttgttg
     1621 gtgcaccact cacccagacg atttatggca tgatcgtgat gaaccagatg tctcaactcg
     1681 caatgcaggg cgtcttcctc tggggaattg gggggttctg tggtgcggca atgggccttt
     1741 ccgcatattt ccaggggaag tgcggtgcct gcgcgtgtga ttcgatggga gagaccggaa
     1801 agggttttgg caattacatg attgtccttg gcatggtcga gaccgtcgca ttgctggtga
     1861 tggcattcac gctcggcatc cttggcagtt ttgggacatg agagagccgg atgaaaagcg
     1921 ggagcgtgag ggataactca aatttttttc tttaatttca ggagaccatc gcacatacat
     1981 cccagggatc attccgggac ttgataattc acaacatcat gtgaaatact ccttggtcat
     2041 tatggcaggt agcagccctg gtcctttatc cgcgcccgtg caataacgcg gagaacagag
     2101 attccctgcc caaaaaaacc catttgccgg gagattcggt ttacgtatcc tttgatcgga
     2161 cgataatcac aggtcttctt gagttcactg caacggcacc cgcggtactg ccaatcagga
     2221 gctcggcgaa ccagcctctg ccgacagagc tcatccagat aactgacacg tcctcctcct
     2281 cggcaacctt tgtaatctcg aatgcagggt ttcctagcct cac
//
LOCUS       DAOQVJ010000261         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57335_length_2316_cov_4.195046, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000261 DAOQVJ010000000
VERSION     DAOQVJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57335_length_2316_cov_4.195046"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1123)
                     /locus_tag="PKL26_06015"
     CDS             complement(107..1123)
                     /locus_tag="PKL26_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS83283.1"
                     /translation="MLWIEKYRPRTFEEIIGQEKVVERLKHFASFRAVPHLMLAGPPG
                     TGKSASVECLARELYGDHAGENLTVVPTSDLFMLGKKFLEGEERYAHLYRADESLLSN
                     FKRIIREYSSLRPLDAEFKLLAFEGASSLPREAQQALRRIMERSSRTCRFIYCTSHPS
                     AVIPAIASRCLPVFFSPIDDSLVESHLHAICAAEGNENCPVREDHVGLLVAAARGDLR
                     KAVMLLQVLAESGPDANLLSLAKTETGQVATAAFSAMQAGDVQAATRRLETLMIEYGL
                     SAREVLGELGNVVKRDFNDPHIACVIAETDWILGHSQNEYLQLNALVAKIARETGDAR
                     RPGY"
     gene            complement(1110..1841)
                     /locus_tag="PKL26_06020"
     CDS             complement(1110..1841)
                     /locus_tag="PKL26_06020"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HNS83284.1"
                     /translation="MIPPEHPRYRSLFARERLARYAAEGIVSLEGLSAHGRGEAFDYL
                     LGEKTTASALLAEKTAAALLLTARDPVISVNGNTAGLAAELIAPLQEKSGAKVEVNLF
                     HRTEERIKKISRLLRDHGVRLEEGNAERLLPLSHDRALCQRGGIYQADVILVPLEDGD
                     RCQALREMGKTVIAIDLNPLSRTARSASLTIVDELTRALPNLTSFCASLPRHEAEALA
                     GSLDNTRFLADALETMKEGLSHAMD"
     gene            complement(1838..>2316)
                     /locus_tag="PKL26_06025"
     CDS             complement(1838..>2316)
                     /locus_tag="PKL26_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNS83285.1"
                     /translation="AHEAEIVHRTGLGDVAACMGGGRDCRKGPGIRAEIERTFDIREP
                     LCAVIFGPLPSPEVLGSPEAMARVIRAFPGRCPVDARDFFSLSRQFAMESGLATPQVV
                     EALSACEKKGLPATMTMLGNGVFAYGDEAFPLLSCLGEALVLHPADAGVHLLEVGG"
BASE COUNT          420 a          713 c          720 g          463 t
ORIGIN      
        1 ggtcagattt tcatcgcttc ggtgtgaagc ttcaacttca tgcaggtttt tttagcgggt
       61 ggcgcaggac actcaacccc tgctgccttg ccaggtgctt gactgactaa tacccgggac
      121 gccgggcatc ccccgtttcc ctggcaatct ttgccaccag tgcattgagc tggagatact
      181 cgttctggct gtgccccagg atccagtcgg tttcggcgat tacacaggcg atatgcgggt
      241 cgttgaagtc gcgcttcacg acgttgccaa gctcgccgag cacctccctc gcggagagcc
      301 cgtactcgat cataagcgtc tccagcctcc gggtggctgc ctggacgtca cctgcctgca
      361 tcgcggagaa cgcggcagtc gccacctggc cagtctcggt ctttgcgagg gagaggaggt
      421 ttgcgtccgg acccgactcg gcaagcacct ggaggagcat gacagccttc cggaggtccc
      481 ctcgtgcggc ggcaaccagg aggccgacat ggtcttcccg gacgggacag ttctcgttgc
      541 cctcagcggc gcatatggca tgcaggtggg attctaccag ggagtcgtcg ataggagaga
      601 agaataccgg caggcaacgg gacgcgatgg cagggatgac tgccgaggga tggcttgtac
      661 agtagatgaa cctgcacgtc cgcgaagacc gctccatgat ccgacgcagc gcctgctggg
      721 cttcgcgggg aagagatgag gcaccctcga atgccagcag cttgaactcg gcatccaggg
      781 gacgaagcga cgaatactcc cggatgatgc gcttgaagtt cgagagcagg ctctcgtccg
      841 cgcggtaaag gtgcgcatac cgctcttcgc cttccaggaa tttcttcccg agcatgaaga
      901 ggtcgcttgt gggaacgaca gtgaggttct cccctgcgtg gtccccgtac aattcccgcg
      961 caaggcactc gacgcttgcg ctctttcctg tgccgggggg accggcaagc atcaggtgag
     1021 gtacggcccg aaacgaggca aaatgcttca gccgttccac caccttctcc tgcccgatga
     1081 tctcttcgaa cgtacggggg cggtacttct caatccatag catgggagag tccctccttc
     1141 atcgtttcga gggcatccgc caggaacctg gtattgtcaa gcgaccctgc aagcgcctcg
     1201 gcttcgtgcc ttgggagcga tgcgcagaac gaggtgaggt tggggagggc ccttgtgagt
     1261 tcgtcgacaa tcgtgaggct tgcactccgg gcggtcctcg aaagtgggtt gaggtctatc
     1321 gcgatcactg tcttgcccat ttcccgaagc gcctggcaac gatcgccgtc ttcgagcggc
     1381 acaaggatga catctgcctg gtagatcccc cctctctggc acaatgcccg gtcgtgggag
     1441 agcgggagga gcctctcggc atttccttcc tccagtcgga caccatggtc gcgaagcagg
     1501 cggcttatct tctttattct ctcctcggtc cggtggaaga ggttgacctc cacttttgcg
     1561 cctgacttct cctgcagagg agcgatgagt tctgcagcca ggcctgcggt gttgccgttc
     1621 acagagatga cagggtccct tgcggtgagg aggagtgctg cggccgtctt ttctgcgaga
     1681 agcgcgcttg cagtggtctt ctccccgagg aggtagtcga acgcctcgcc gcgtccgtgg
     1741 gcagaaagtc cttcgagcga gacgattccc tccgcagcgt aacgtgcaag ccgctccctt
     1801 gcgaagagcg atcggtaccg ggggtgttcc ggcggtatca tccccccacc tccaggagat
     1861 gcacccccgc atcggcaggg tgaagcacca acgcctcgcc caggcatgag agcaggggga
     1921 acgcctcatc tccgtatgcg aagaccccgt ttccgagcat agtcatggtc gcgggcaacc
     1981 ccttcttttc gcaggcggag agtgcctcga ccacctgcgg cgtggcaaga ccgctctcca
     2041 ttgcaaactg cctggagagg ctgaaaaagt cccgcgcgtc caccgggcag cggcccggaa
     2101 aggccctgat gaccctggcc atggcttccg gggagccaag tacttccggg gatgggagcg
     2161 ggccgaagat cacggcacag aggggttcgc ggatgtcaaa ggtgcgctcg atctcggccc
     2221 tgatccctgg acccttcctg cagtcccggc cgccccccat gcaggccgca acatctccaa
     2281 gtccggtcct gtggacgatc tctgcctcgt gggcca
//
LOCUS       DAOQVJ010000262         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57451_length_2313_cov_4.758636, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000262 DAOQVJ010000000
VERSION     DAOQVJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57451_length_2313_cov_4.758636"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKL26_06030"
     CDS             <1..341
                     /locus_tag="PKL26_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS83286.1"
                     /translation="QVVRDTGVLVNVERALIDSSEGEALIDIPDESCRIVKDKLADLG
                     ASVRILEEEISLDETECVDCGACISICPQEVFSFDDEFRLRMDAKRCVLCGRCIEICP
                     HGALSRRVDI"
     gene            complement(448..1755)
                     /locus_tag="PKL26_06035"
     CDS             complement(448..1755)
                     /locus_tag="PKL26_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNS83287.1"
                     /translation="MPHQRPAGHRRRRTGHYSPPVYLVTVQVGSQWTRECMARLATIF
                     GLLPPERHAPHITLFGPFTLDKGCDIRVLLEDPLLRSPGFSSFSAMLGGALVLRGRKG
                     YAAVIRAAPGDPLALLAAAVRDSLLPHTRTCTWIDQIAGQRIFHVSTGFGLRRRKAEE
                     IVEFLDTLPPGRRNAEGMRCMAGTTLDLFRLEVIRKGTLMDAFDFPTGTWIGRPAAFS
                     EDRWEKTLESFRQKSGYQIDHPSFSEEDTAFVISDLHLGHANIITYTSRPFPDAATMD
                     SVLIQNWNFRVRPTDTVYFLGDLAYGRNAGPAARYLSLLEGVIHIVAGNHDSGLEGAS
                     GSMEITWRSRRFLMVHDPAEAPLAYPGFVVHGHLHNNQPGEYPFLNMPGRRVNVSAEM
                     VGYVPLSLDELVDIIETSPGDAQFPTLNDARRKLNRWRESHRP"
     gene            1859..>2313
                     /locus_tag="PKL26_06040"
     CDS             1859..>2313
                     /locus_tag="PKL26_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNS83288.1"
                     /translation="MIRRRFHFRETIATILAEEESHVQAAREGLMQARQELEHYIAFD
                     PFFRTTMEPYEAVSDSTVVKRMAAAGASAGVGPMAAVAGAIAWAGVESMARTGAEFAV
                     IDNGGDIALLSDRVLLVGVHAGRSPHSDRFAFQVTPRAEVLGICTSSATV"
BASE COUNT          432 a          666 c          724 g          491 t
ORIGIN      
        1 cccaggtggt gcgcgatacc ggggtgctgg tgaacgtgga gcgggccctc atcgattcat
       61 cggagggaga ggcgctcatc gacatccctg atgagtcctg caggatcgtg aaagacaagc
      121 tggcagatct cggcgcctcg gtgcgcatcc tcgaggagga gataagtctc gatgagaccg
      181 agtgcgtgga ctgtggcgcc tgcatcagca tctgccccca ggaggtcttc tcctttgacg
      241 atgagttccg gctccggatg gatgcaaaac gctgcgtcct ctgcggcagg tgcatcgaga
      301 tatgccccca cggggctctc tcgcgccggg tagatatctg actcggatcg gcaggagaca
      361 tcggttttct catggtatct ttcaaccatt ttttatgtgg tatgcgcgtg ttttgcggac
      421 gaatcccctt ttttatccgg ttggcactta gggcctgtgg ctctctctcc atcgattcag
      481 tttccggcgg gcatcgttga gcgtcgggaa ctgtgcatct cccggcgagg tctcgataat
      541 atccacgagt tcatcaagcg agagggggac ataccccacc atctccgcgc tgacgttcac
      601 cctcctgccc ggcatgttca ggaacggata ttctcccggc tggttgttgt gcaggtgtcc
      661 gtgaaccacg aatccaggat aagccaatgg tgcctccgcg ggatcgtgca ccatcaggaa
      721 gcgccggctc ctccaggtga tctccatgct cccgctggcg ccttcaagcc ccgaatcgtg
      781 gtttccagcc acgatatgga taaccccttc caggagggac aggtagcgtg cagcaggtcc
      841 tgcgttccgc ccatatgcga ggtcgcccag gaagtagacc gtgtcggtgg gcctcacgcg
      901 gaagttccag ttctggatga gcaccgagtc catggttgcg gcgtctggga agggccggga
      961 ggtgtaagtg atgatattgg cgtgacccag gtgcaggtct gagatgacaa acgcagtgtc
     1021 ttcctcgctg aaagaagggt ggtcaatctg gtacccgctc ttttgccgga acgattcgag
     1081 ggtcttttcc cacctgtcct cactgaacgc ggccgggcgg ccgatccacg tgcccgtggg
     1141 aaaatcaaat gcatccatga gcgtcccctt cctgatgacc tcgaggcgaa acaagtccag
     1201 ggtcgtcccc gccatgcagc gcatgccttc ggcatttctt ctcccgggtg gcagagtgtc
     1261 caggaattcg acgatctctt ctgccttcct gcggcgcaac ccaaaccctg tgctcacgtg
     1321 aaatatccgc tgtcctgcga tctggtcgat ccaggtgcag gtccgggtgt ggggaaggag
     1381 agagtcccgg acagcggcgg cgagaagggc cagggggtcg cccggggccg ccctgatgac
     1441 cgcagcatat ccctttcgtc cccggagtac cagtgcaccc ccgagcatgg cggaaaagga
     1501 ggagaagccg ggtgaacgca gaagtggatc ttcgaggagc acccggatgt cgcagccttt
     1561 gtccagggta aatggcccaa agagcgtgat gtggggggca tggcgttctg gggggagaag
     1621 tccgaatatt gttgccaggc gcgccatgca ctccctggtc cactggctgc cgacctgcac
     1681 ggtcaccagg tatacgggag gggagtagtg accggtcctc cgccttcggt ggccggccgg
     1741 tctctggtgc ggcattctat ttccagaaga tcgcttgata tatgcgtata aatagatgct
     1801 gacagcgtat cgggtgatcc gagccctttt ttctctccca ggagaattac tctcatctat
     1861 gatcaggcgg cggttccatt tccgcgagac gatcgccacc atccttgcag aagaggagag
     1921 ccacgtccag gcagcccgcg aggggttgat gcaggcaagg caggagctcg agcattatat
     1981 cgcattcgat cctttcttca ggaccacgat ggagccctat gaagcggtat cagacagcac
     2041 tgtcgtgaaa agaatggccg ccgcgggagc ttctgccgga gtcggcccaa tggccgcggt
     2101 ggcaggcgca atcgcgtggg caggcgtgga gtcgatggcg aggaccgggg ctgaattcgc
     2161 cgtgatcgac aatggcgggg acatcgccct cctctctgat cgcgtccttc ttgtgggggt
     2221 ccacgccggc cggtcacccc acagcgaccg cttcgcgttc caggtaactc cccgcgcaga
     2281 ggttctcggg atctgtactt cttctgccac agt
//
LOCUS       DAOQVJ010000263         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57453_length_2313_cov_4.389283, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000263 DAOQVJ010000000
VERSION     DAOQVJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57453_length_2313_cov_4.389283"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PKL26_06045"
     CDS             <1..1639
                     /locus_tag="PKL26_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HNS83289.1"
                     /translation="GFILDGVSLKLGSPLWLGTRAFTIPALFAFLVTKPAVELIGRPM
                     TWNRSALLALSCTLFGVIITLSSLTISQSMLPLFYAISLGIILGLRLLVLVAIADYRV
                     LRMILPAASQSLAGIAMGMVLFPGNFFVFALVLQVFFGLGFILLVWAIDRPLYRTFQI
                     RGLDFLNSFLAHLTDGSKALEDFFRKIGEEVTVPQVTLFFRRNEKTGLLLTVPNVHPG
                     PMGEIGGGNLPRGMQSGFSEMVMVPHGAATHDFNLVSEQEIDKLVQAVAASRDNLVFS
                     SSATRSVRYQEGSVSLLCQVFRDTLLMVSTRSPERTEDLDFNIGMTIMAEGHRSFPHI
                     AFIDAHNCYAGDITYVLPATRLAMEYYHAGIKAIDETPGMPRFSLELGIAHVQVPFSR
                     EQGFGDQGIQLAVVRAGEQSTAYLLIDGNNMESGVRETIRNFLLGSVDEAEVMTTDSH
                     VVNTVSGKNPVGLHVPVSEIIPCVDAALRQALADIAPAEAAGSTAWCEGVVVFGSHRI
                     TQMASTVNTILLFIPVLSAGMLLLVFLLSILVYVMIG"
     gene            2131..>2313
                     /locus_tag="PKL26_06050"
     CDS             2131..>2313
                     /locus_tag="PKL26_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83290.1"
                     /translation="MKKNNRITGDIMRQAPVSVFTRAGTARRSGKGMSLSLGDRIFYV
                     PPEEVGGLFSDRHVMIL"
BASE COUNT          487 a          658 c          615 g          553 t
ORIGIN      
        1 cgggttcatc ctggatgggg tcagcctgaa actcggatcc ccgctctggc tcgggacgcg
       61 tgccttcacc atcccggccc tcttcgcatt ccttgtgacg aagcccgcgg tggaactgat
      121 cgggcgtccc atgacctgga acaggtccgc gctcctggcg ctctcctgta ccctcttcgg
      181 ggtgatcatc accctctcat ccctgacgat ctcccagtcg atgctccccc tcttttacgc
      241 tatctcactg ggcataatcc tgggcctccg gcttctcgtc ctggttgcaa tcgcggatta
      301 ccgggtcctc cggatgattc tcccggcagc aagccagagc ctggcgggga ttgccatggg
      361 aatggtcctt tttccaggga atttttttgt ctttgcactt gtcctccagg tgttcttcgg
      421 cctcggcttt atcctgctcg tatgggcaat cgacaggccc ctttaccgta catttcaaat
      481 caggggcctg gattttctca actcgttcct tgcgcacctg acagacgggt caaaagccct
      541 cgaggacttc ttccgcaaga tcggggagga ggtgaccgtg ccgcaggtca ccctattctt
      601 tcgcaggaac gaaaagactg gcctcctcct cactgtcccg aacgtgcacc ccgggcccat
      661 gggggagatc gggggcggaa acctcccaag ggggatgcag tcgggattct ccgagatggt
      721 gatggtcccg cacggggccg ccactcatga cttcaacctt gtatctgagc aagagattga
      781 caagctcgtg caggcagtgg ccgcatccag ggacaacctc gtcttttcta gttccgcaac
      841 ccgttcggtc cggtaccagg agggatcagt atcactcctc tgccaggtct ttcgcgatac
      901 tctcctgatg gtaagtacca ggtcacccga acggaccgag gaccttgact tcaacatcgg
      961 aatgaccatc atggccgagg ggcaccggtc cttcccgcac atcgcgttca tcgatgccca
     1021 caattgctat gccggggata tcacctatgt cctccccgcg accaggcttg cgatggagta
     1081 ttaccatgcg gggataaaag cgattgacga gacacccggg atgccgaggt tttcgctgga
     1141 gcttggcatc gcgcatgtgc aggtcccctt ctcccgcgaa cagggatttg gagaccaggg
     1201 catccagctc gctgttgtgc gggcaggaga gcagagcacc gcctaccttc ttattgacgg
     1261 gaacaacatg gagagcgggg taagggagac catacggaat tttttactcg gatcagttga
     1321 cgaggcagaa gtgatgacga ccgattccca cgtggtcaat acggtgagcg gaaagaatcc
     1381 cgtcggcctc catgtcccgg tctccgagat catcccgtgc gtggatgcgg cgcttcgcca
     1441 ggccctcgca gacattgccc ctgcagaagc cgcggggagc actgcctggt gcgagggggt
     1501 cgtggtattc ggttcccatc ggatcaccca gatggcgagc accgtcaata ccattctcct
     1561 cttcatcccg gtactgagtg caggaatgct cctcctggtc ttcctcctct ccatccttgt
     1621 ctatgtcatg attggctgac ggtggttggg aaaaaaacga aaaaaacgcg gatgacctga
     1681 tttacgactt ttcttaagga gatgtctatt ggcagaagga gaactcaaag gaaatgacca
     1741 ccatggtcca ggcagtggtt cttcaattga tgcgtgagag tatcaggcaa cgatcaattg
     1801 gagtatcagc attaactgac actgaaccat gttggtttca cattttttgt tgtattgtcc
     1861 tccatataaa ctttttggaa aataatcgca aatcacttta caatctctac ttgaggcaaa
     1921 aatgcaggca ttttgtctga gaagcgcaca atctgtcaga caaatgtctg acggattatg
     1981 tgccacttgc tgacgccacc gattcccaaa gcggtctttt cgcatggcgc acacgcagat
     2041 ccgaccccgc atctgtgaag tttgaatcct gcattttaac gtttattcat caatctgaac
     2101 ccaatttgtc gtctgacgcc aggtttacat gtgaagaaga acaacagaat aaccggtgac
     2161 atcatgaggc aggcaccggt atctgtcttt acccgggcgg gcacggcccg gcgttcagga
     2221 aaagggatgt ccctttccct tggggaccgc atcttctatg ttccccccga agaggtaggt
     2281 ggactgtttt cagaccggca cgtaatgata ctc
//
LOCUS       DAOQVJ010000264         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57531_length_2311_cov_4.007535, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000264 DAOQVJ010000000
VERSION     DAOQVJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57531_length_2311_cov_4.007535"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKL26_06055"
     CDS             <1..471
                     /locus_tag="PKL26_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS83291.1"
                     /translation="EIGIEAGLATLLVGFFAIFNGGGRPVFGSLTDRITPRNTALASF
                     ILIALASLALWQVPAIPVYILAFAILWGCLGGWLAIAPTATGTYFGTCDYPRCYGLVF
                     LAYGAGAIAGPQLAGFIKTTTGNYMGVFPYVLALSLIGLAIAWVLVKAPKKMTS"
     gene            584..1201
                     /locus_tag="PKL26_06060"
     CDS             584..1201
                     /locus_tag="PKL26_06060"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNS83292.1"
                     /translation="MEEVEKSRLEALSDGIFAFAMTLLVISLAVPDIPYEQAPRVLPA
                     MIAGMAPEFLLFVIAFFILAGYWISHHRILEQVKYVDGVLVRINILLLFFIVLIPFTT
                     SISGDYTNVLEAVLFFHVNLLLASFTLSGMGWYIKRHMAAFAPGVQPPWKSGLEKAVV
                     MHGVILLAIGVSFVNTRASMWCYALIPIIYFVLGAIHRILEAEKR"
     gene            1198..1953
                     /locus_tag="PKL26_06065"
     CDS             1198..1953
                     /locus_tag="PKL26_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS83293.1"
                     /translation="MKGLDILYRIHSGLPRQGPGSTECTARAFRHVNLQNMHPLVLDI
                     GCGSGASALVLSRLTGGTVVAVDNHQPFLVELKERAWALQGPGRKEVQPLQASMSSLP
                     FRNGSFDLIWSEGAAYLMGFEEALLSWRPLLKREGFLVVSEVTWLECNPPEEIRSYWE
                     ANYPAIASDSSHIETIARSGYVLHRSFTLPESTWWNYYRPLQGKMRKFRREYADDPVA
                     QSVLDEIDEEIGMYENYHGSYGYTVYVMEKRDD"
     gene            1946..>2311
                     /locus_tag="PKL26_06070"
     CDS             1946..>2311
                     /locus_tag="PKL26_06070"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNS83294.1"
                     /translation="MIDREKETVRESGLPPLIGPSPGVLVLGSFPSRMSLAAGRYYAN
                     PRNHFWPIMQQVLSLEGEGIPEWEEGLKSAGVAVWDVIASRRFQPGSMDRDICDEEWN
                     DILGLLVTHPTIRCICLNGG"
BASE COUNT          455 a          666 c          657 g          533 t
ORIGIN      
        1 gagatcggaa tcgaggcggg gcttgctacg ctgctcgtcg ggttctttgc tatcttcaac
       61 gggggcggcc ggccggtctt cgggagcctc accgaccgga tcaccccgag aaacactgcg
      121 cttgcctctt ttatcctcat cgcactcgca tcgcttgcgc tttggcaggt gccggctatc
      181 ccggtctaca tccttgcgtt cgccatcctg tggggatgcc tgggcgggtg gcttgccatc
      241 gccccgacgg ccactgggac gtacttcggg acctgcgatt acccacggtg ctacggcctg
      301 gtcttcctcg cctacggggc aggggcaatt gccggccccc agctcgcggg gttcatcaag
      361 acgaccaccg ggaactacat gggggtcttt ccttacgtgc tcgcgctctc cctgataggg
      421 ctcgcgatcg cctgggtgct tgtgaaggcg ccgaagaaaa tgacttcctg attcattcct
      481 ttttttcccc ctttgatcgt tccctgttcc ggcacacccg gattaaatag ggggaattcc
      541 tacctcatat ccagcagaca ccaggcagcg ggagagtcac gcgatggaag aagtggagaa
      601 gtcaaggctt gaggcgctga gcgatggcat ctttgccttt gcaatgaccc tgctcgtcat
      661 atcgctggcg gttcccgata tcccgtatga acaggcaccc cgcgtcttac cggctatgat
      721 tgcaggaatg gcccccgaat tcctgctctt cgtgattgcc ttctttatcc ttgcaggcta
      781 ctggatctcg catcaccgga tcctggaaca ggtgaagtat gttgacgggg tgcttgtacg
      841 gatcaacatc ctcctcctct tcttcatcgt gctgatacca ttcaccacat cgatatcagg
      901 agattatacc aacgtcctcg aggcggtcct cttcttccat gtcaacctcc tccttgcaag
      961 cttcactctt tcaggcatgg gatggtacat aaaacgccat atggcagcat ttgctccagg
     1021 ggtgcaacca ccatggaaga gcggtcttga aaaagccgtc gtcatgcatg gggtgatact
     1081 tcttgcgatc ggtgtgtcgt tcgtgaacac ccgcgcaagc atgtggtgct atgcgctcat
     1141 ccccatcatc tatttcgtgc ttggagccat ccatcgtatc ctggaggcgg aaaagagatg
     1201 aaagggctcg atatcctgta ccggatccat tccggccttc cacgacaggg gccgggaagc
     1261 accgagtgca cggcccgtgc gttccggcac gtcaatctcc agaacatgca tccactcgtc
     1321 cttgacatcg ggtgcggttc cggtgcatca gccctcgtcc tctcacgcct cacaggtgga
     1381 accgtggttg ccgttgacaa tcaccagcct ttcctcgttg agctgaagga gcgggcctgg
     1441 gcgctccagg gcccggggag aaaagaggtg cagccgttgc aggcctcgat gtcgtccctc
     1501 ccattccgca acgggagttt cgacctgatc tggagcgagg gggctgcgta tctcatggga
     1561 tttgaagagg cgctcttgtc gtggcggccc cttctcaaaa gagaggggtt ccttgtcgtt
     1621 tctgaggtga catggctcga gtgcaatcct ccagaagaga tccgctctta ctgggaggca
     1681 aattatccgg caatagcatc cgattcctca cacatcgaga cgatagcccg ttcgggatac
     1741 gtcctccatc gctccttcac gctcccagaa tcgacctggt ggaactatta tcgccccctc
     1801 caggggaaga tgaggaaatt ccgcagggaa tatgcagacg acccggtcgc acagtcggtg
     1861 cttgatgaga tcgacgagga gattggaatg tacgagaact accacggttc gtatggctat
     1921 acggtgtatg tgatggagaa gcgggatgat tgaccgcgaa aaagagactg tcagggaatc
     1981 cgggcttcct cctctcatag gcccctcgcc gggagtcctt gtcctgggaa gcttcccgag
     2041 caggatgtcc cttgccgcag gtcgttacta tgccaaccca agaaaccatt tctggccaat
     2101 catgcaacag gtcctctctc ttgagggcga agggataccg gaatgggagg agggactaaa
     2161 aagcgcaggt gttgcggtct gggatgtgat cgcctcccgc aggtttcagc cgggaagcat
     2221 ggaccgcgac atatgcgacg aggaatggaa cgatatcctg ggcctccttg tcacgcatcc
     2281 cacgatccgg tgcatctgtt tgaatggtgg g
//
LOCUS       DAOQVJ010000265         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57535_length_2311_cov_3.350177, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000265 DAOQVJ010000000
VERSION     DAOQVJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57535_length_2311_cov_3.350177"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PKL26_06075"
     CDS             <1..1237
                     /locus_tag="PKL26_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS83295.1"
                     /translation="PAECVPVQATDPLYILYTSGTTGIPKGVVRDNGGHLAALLWSMR
                     YLYGLSPGEVFWAASDVGWVVGHSYIVYGPLAYGSTSILYEGKSVGTPDPGAFWRVAS
                     QHGVNVLFCAPTALRAIKREDPGGKYLERHDLSRMRTLFLAGERCDPDTLAWAKARLS
                     IPVIDHWWQTETGWAIGANPVGLTMLPVKPGSCTRAMPGFDVRVLDDRGIETPVGVTG
                     NIVIRLPLPPGCFTTLWQKDDECVRTYFSAYPGFYLTGDAGFIDEDRYLWIMGRTDDI
                     INTAGHRLSTGAMEEVLASHPDVAECAVTGVADEVKGEIPVGFVVLKSGVRRENGDVV
                     QELIQMVRDRIGPIASFKAAVVVRRLPKTRSGKILRGVMKKIADGERYSIPPTIEDQA
                     VLGEVAESLMSIGYPRRAR"
     gene            1398..>2311
                     /locus_tag="PKL26_06080"
     CDS             1398..>2311
                     /locus_tag="PKL26_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNS83296.1"
                     /translation="MLIGMDAGGTNTDLAMVDREVTSVKVPNTLGIREVLSHARAPGR
                     LAVSTSTPLNRVLTGDPARVQTILIPGPGLFWPGGIRGAVNHRGDIVEHLDSTEVGGV
                     LASSRGDVLAITGKFSVRNPSLEQEVAARALDHYAEERIAVSHHLATLDFPGRAATTR
                     LNARMKEAVCLLAGEIGAERKDFFFFRGDGGLIPPAYAIRDPSVLYHSSTAAAALGAF
                     YLSKVEECLVVDIGGTTTDLVRLSGGKPAMETIVADGERTVTRAVAAITLPYGGDSVI
                     EETMQPRRMGNALAFGGIRPTLTDALNVT"
BASE COUNT          445 a          672 c          753 g          441 t
ORIGIN      
        1 gcctgcagaa tgcgttcccg ttcaggccac cgatcctctc tatatcctct atacatctgg
       61 gacgacaggt atcccaaaag gagtcgtgag ggacaacggc ggtcatctcg cagcgctcct
      121 ctggagcatg agatacctct acggcctgtc gccgggagag gtcttctggg cggcatctga
      181 tgtcggatgg gtggtggggc actcctacat tgtctatgga cccctcgcct atgggagcac
      241 gagcatcctc tatgagggga aatccgtggg aacacctgat cccggggctt tctggcgcgt
      301 cgcgtcccag catggagtga acgtgctctt ctgcgcaccg actgcgcttc gcgccatcaa
      361 gcgggaagac ccggggggaa aatacctcga gaggcacgac ctgtcccgga tgagaaccct
      421 cttcctcgcc ggggagcggt gcgaccctga cacccttgcg tgggcgaaag cgcgcctctc
      481 gatccccgtc atcgatcact ggtggcagac tgagactgga tgggcaatcg gggccaaccc
      541 tgtcggactt accatgcttc ctgtcaagcc cggctcgtgc acccgggcga tgccaggctt
      601 cgatgttcgt gtcctcgatg acaggggaat cgagaccccc gtgggggtca cgggaaacat
      661 cgtgatccgc ctgccgcttc cccccggctg ttttaccacg ctctggcaga aggacgacga
      721 gtgcgtccgg acatacttct cggcataccc gggattctat ctgacaggcg atgccgggtt
      781 cattgacgag gacaggtatc tctggatcat gggcaggacc gatgacatca tcaacaccgc
      841 agggcaccgg ctctctaccg gtgcgatgga agaggtgctg gcatcgcacc cggacgtggc
      901 ggagtgtgcg gtcaccggcg tggcggacga ggtgaagggg gagatcccgg tggggttcgt
      961 tgtactcaag tccggggtgc ggcgtgagaa cggcgatgtc gtccaggagc tgatccagat
     1021 ggtcagggat cgcatcggtc ccatcgcatc gttcaaggcg gcggtcgttg tcaggcgcct
     1081 tcccaagaca cgttcaggga agatactccg gggcgtgatg aaaaagattg cggacggcga
     1141 acgctattct atccccccga cgatagagga ccaggcggtc cttggcgagg tggcagagag
     1201 cctcatgagt atcgggtacc cccgcagggc ccggtgaaca gtccttgtgc aagggcactc
     1261 atcacgccat tccggggcgc cgattggtga tgccggcggg accctggcac tccccatctg
     1321 ttcacgtgcc cgaaaaggat accagagatt tttttttggt accaggatga acgtgtggtg
     1381 aagggggtca tcaaaagatg cttatcggga tggatgccgg cgggaccaac acggatctcg
     1441 ccatggttga ccgcgaggtc acatccgtca aggttccaaa cacactcggc atccgggagg
     1501 tgctgtcaca cgccagggcg cccggccgcc tggcggtaag cacctcgact ccgctcaacc
     1561 gggtgctcac cggtgatcca gccagggttc agacgatcct gatacccggg ccaggtctct
     1621 tctggcccgg aggtatcagg ggcgcggtga accaccgggg cgatatcgtg gagcacctcg
     1681 actccacgga ggtcggcgga gttcttgcct cctcccgtgg cgatgtcctt gcaattaccg
     1741 gaaagttctc ggtgcgaaat ccttcgctcg agcaggaggt ggcggcccgt gccctggacc
     1801 actatgccga ggagcggatt gccgtaagcc atcaccttgc cacccttgac ttcccgggga
     1861 gggcggcaac cacgaggctg aatgcacgca tgaaagaggc ggtctgcctc cttgcaggag
     1921 agattggggc tgaaaggaag gactttttct tcttcagggg cgatgggggg ctcattccac
     1981 cagcctatgc aatcagggac ccttccgtcc tctaccattc gagcacggca gcggccgcac
     2041 ttggcgcatt ttacctgagc aaggtcgagg agtgcctggt agtggatatc gggggcacca
     2101 ccacggacct ggtcagactt tcagggggaa agcccgcgat ggagacaatc gtagccgatg
     2161 gcgaacggac ggtgacgcgt gctgtcgcgg cgataacgct cccttatggc ggggactcgg
     2221 tgatagagga gacaatgcag ccccggcgga tgggaaacgc ccttgcattt ggggggattc
     2281 ggcccactct gacggatgcg ctgaacgtga c
//
LOCUS       DAOQVJ010000266         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57604_length_2309_cov_3.850044, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000266 DAOQVJ010000000
VERSION     DAOQVJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57604_length_2309_cov_3.850044"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKL26_06085"
     CDS             complement(<1..216)
                     /locus_tag="PKL26_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83297.1"
                     /translation="MQAPLNPWSSQQSDDIEKLFSMFGIEPIATIEGVLPRVPSFISR
                     GVVVGHRDYRQIAEAIHGSAVRQHRFGV"
     gene            complement(228..1235)
                     /gene="endA"
                     /locus_tag="PKL26_06090"
     CDS             complement(228..1235)
                     /gene="endA"
                     /locus_tag="PKL26_06090"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HNS83298.1"
                     /translation="MKATFDGTWVRAGHEGRALYDQSGYGRPEKEGIRLAPEEALYLV
                     HRGRLEVAGYSFDRLLAVCAERPEFMRSYLVYRDIRERGYVVQTGPHDFRVFRRGERP
                     GTGQSQYLVRVISERDLIDFSGLLGEAAASLNLRKQHVLAVVDDENELTYYEVKMPTL
                     PQVEKEEEEWNTRGELVGKYAIVHVPPSGSAVPGSYGMQLDPGRLVLAPLEILNLMRS
                     GRLTLQGNGEPIDPERYYRMAHESDIEFPEKVAVYEDMRNRGFVPRTGYKFGHHFRVY
                     SGKKVHSEMLVQALPAGASLPMSAISRSVRLAHSVKKKMLFGCEHSQGIQYVEFARIK
                     L"
     gene            1609..2193
                     /locus_tag="PKL26_06095"
     CDS             1609..2193
                     /locus_tag="PKL26_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HNS83299.1"
                     /translation="MINCAGFLERSAVNGPGRRAVVWVQGCPLRCPGCFNRDLWSFRK
                     ANFVHEEMLAERILSIPGIEGVSFSGGEPFCQAAPLATLGKIIRDAGRSVVTFSGYPI
                     ENLLRSRRQDWRALLQETDLLVAGPYLQELRMESSLCASSNQVAAFLSGRITPYQEEA
                     DKGESVEFTICRDGEITTTGFPKRAPLPFERGCT"
BASE COUNT          469 a          678 c          668 g          494 t
ORIGIN      
        1 gacaccaaaa cggtgctgcc ggacggcact gccgtggatg gcctcggcga tctgccggta
       61 atcgcggtgg ccgaccacga cgccgcggct gatgaacgac ggcacgcggg ggaggacccc
      121 ctcgatggtg gcgataggct cgatgccgaa cattgagaag agcttctcga tatcgtcgga
      181 ctgctggctg gaccaggggt ttaagggtgc ctgcatgcgt tatctactca cagtttaatc
      241 cgggcaaatt ccacgtattg gattccttga ctatgttcgc acccgaacaa catcttcttt
      301 ttcacactgt gagccaggcg gacggacctc gatatggcgc tcatcggaag gcttgcgcct
      361 gcaggaaggg cctgcaccag catctcggaa tggaccttct ttcctgaata gacacggaag
      421 tgatggccga acttgtagcc ggtccggggt acgaacccgc ggttcctcat gtcttcgtag
      481 accgcaacct tctccgggaa ctcgatgtcg gactcgtgcg ccattctgta atagcgctcg
      541 gggtcaatcg gttctccgtt cccctgcagt gtgagccggc ccgagcgcat caggttcagg
      601 atctcgagcg gagcgagcac gaggcggccc gggtcaagct gcataccgta actgccggga
      661 accgccgacc cggatggcgg gacatggaca atcgcatact tcccgaccag ttcgcccctg
      721 gtgttccatt cctcctcctc cttctccacc tgtgggagtg tcggcatctt cacctcgtaa
      781 taggtcagct cgttctcatc atcgaccacc gcaaggacgt gctgctttcg caggttgagc
      841 gaggctgccg cctctccgag aagcccggag aagtcgatca ggtccctctc ggagattacc
      901 ctgaccaggt actgggactg gcccgttcca ggcctctctc ccctccggaa gacgcggaag
      961 tcatgcgggc cggtctgcac gacgtatccc ctctcccgga tgtcacggta caccaggtaa
     1021 ctccgcatga attcgggcct ctctgcgcag acggccagga gacggtcgaa tgaataccct
     1081 gcgacctcga gccttccccg atgaacaaga tagagggcct cttcgggcgc gagccgtatt
     1141 ccctcctttt ccggcctgcc atacccgctc tggtcgtaga gtgcccgccc ctcgtggcct
     1201 gcccgcaccc acgtcccgtc aaaagtcgct ttcactgtat acccattgga agaatgcgct
     1261 cataatatca tgccgcgatg cagtatgcct tatggaacag gattacccca aaaacaggca
     1321 tcagatcact cttatagaat accaggagat atgccctgaa tacccttttg tgggatatat
     1381 ataactcctt tttgcatttc gaagagattc tggctgcgcg agggggccag gttgccaggg
     1441 gaattgcatt acttatgtaa tacatttgta gtactttttc gcggaaaaac ggccgcagag
     1501 agaaggactt ataccattcc ggctggaaac ctctcttttc atgtatcagg gggagattgc
     1561 cccgggggaa tgcgatggcg gttttcgacc aggcggggtg aaggggatat gatcaattgt
     1621 gccgggtttc tcgagcgatc cgcggtgaac gggcccggga gaagagcggt cgtatgggtc
     1681 caggggtgcc cgctccgctg ccctggttgt ttcaaccggg acctttggtc attcaggaag
     1741 gcaaatttcg tccacgagga gatgctggcg gagcgcattc tctccatccc cgggatcgag
     1801 ggggtgtcgt tttccggcgg ggaaccgttc tgccaggcag ccccccttgc aacactcggg
     1861 aaaataatcc gcgatgcggg caggtccgtc gtgacgttca gcgggtatcc gatagagaat
     1921 ctcctgcgaa gccggcggca ggactggagg gcgctcctgc aggaaaccga tcttctcgtg
     1981 gcaggaccct acctccagga actccgcatg gaatcatctc tttgtgcctc ttcaaaccag
     2041 gtggcagcct tcctttccgg gaggatcact ccttatcaag aagaggcaga caaaggagag
     2101 tcagtcgagt tcaccatatg ccgcgatggg gagatcacca ccaccgggtt cccgaagagg
     2161 gcccctcttc ctttcgagcg ggggtgcacc tgatccatgt cgcacttctc aaaagtgaag
     2221 accgccttca aggaccagtc tcttctcgca caatgcctcg aggagatggg ctaccaggtc
     2281 gagacagggg ggaccatcag gggttacca
//
LOCUS       DAOQVJ010000267         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_57721_length_2306_cov_3.276322, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000267 DAOQVJ010000000
VERSION     DAOQVJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57721_length_2306_cov_3.276322"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKL26_06100"
     CDS             <1..571
                     /locus_tag="PKL26_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS83300.1"
                     /translation="TSIHVANTVEEDFTTVYPDQDLDVVIPLLQAYKLVVVYDEDHRL
                     VGQVSAGDVLRVMRPQGTLPDVMEPACTIDVDERVVHWRRRMIDDNLHRCVVTENDQV
                     VGIVTETDVAVAMRRFREMVEDRHQDHRIRNLLVKDIMTSPVLTVDMSASIDEVVDLM
                     HQRGISSIPVTNGGKIAGLVTRGSLIKAL"
     gene            688..1779
                     /locus_tag="PKL26_06105"
     CDS             688..1779
                     /locus_tag="PKL26_06105"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNS83301.1"
                     /translation="MAGKKEEHPGQPVGIESIMGGPLKKVERYGMGATQPVVPSDNVA
                     ELLQGMAKTGFQGRKLGESVEVWKEMLRDPEVTIFFGLSGAMIPAGMQKILIELARRH
                     YIDVVVSTGANIFHDVCEHLGVCHYLGHHHVSDAGLYEKGIDRIYDVFAFEEEFRWVD
                     RGFANFAERIAPFRGSSREFLQRFGEWLIREVPDGNSFLAACVRERIPVFVPALCDSS
                     IGIGALIARRRGVEIDIDQIADTDEVTSLVEEGRKTGVVYVGGGVPKNFIQQTQVIGS
                     IHEKDLGGHDYAIQYTAGSPHWGGLSGCTFEEAISWGKESAATRHVQVHVDATIALPI
                     VVSALIGSGVPRVGRKGAKRSKVLRQGVD"
     gene            1849..>2306
                     /locus_tag="PKL26_06110"
     CDS             1849..>2306
                     /locus_tag="PKL26_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS83302.1"
                     /translation="MDLPYLRHRILYVDDEPALLDICRLFLEKSGHFEVVTVNSGFLA
                     LDCLAHQHFDAVVSDYQMPGMDGIELLRRVRREGNTIPFIIFTGKGREEVVIQALNEG
                     ADHYLQKGGQPKAQFVELAHKVKTSILKKEAEAALKESEEKYRMLVEINR"
BASE COUNT          514 a          590 c          708 g          494 t
ORIGIN      
        1 cacctcgata catgtggcaa acacggtcga ggaggacttc accacggtct accccgacca
       61 ggacctcgac gtggtcattc ccctcctcca ggcctacaag cttgtggtgg tctacgatga
      121 ggatcaccgc ctcgtggggc aggtctcggc tggagatgtg ctccgggtga tgcggccaca
      181 gggcaccctc cctgatgtga tggagcctgc atgcaccatc gatgtcgacg agcgcgtggt
      241 gcactggagg aggcggatga tcgatgacaa ccttcaccgc tgtgtcgtaa ctgagaacga
      301 tcaagtggtg ggaattgtga ccgagacaga tgtggccgtc gccatgcgga ggttccgcga
      361 gatggtggag gacaggcacc aggaccaccg catccggaac cttctcgtca aggatatcat
      421 gacaagccct gtgcttactg tcgatatgag cgcaagcatt gacgaggtgg tcgacctcat
      481 gcatcagaga ggcatcagtt cgatacccgt gacgaacgga gggaagattg cggggcttgt
      541 caccaggggg tctctgataa aggcccttta accacctttt tctttcgatt gttttgttct
      601 tcaggggttc ttggggtatc cgttctcggt gcaccgtcgc tctgcacggc gcacatttct
      661 tatcaattgc ccgtccatct ctatgcaatg gcagggaaaa aggaagagca ccccgggcaa
      721 cccgtgggaa tcgaatcgat tatgggcgga cccctgaaga aagtggaacg gtacgggatg
      781 ggtgcaacac agcctgtcgt tccttccgat aacgttgccg agctcctcca ggggatggca
      841 aagacaggat tccaggggag gaaactcggc gagtcggtgg aggtctggaa agagatgctg
      901 cgtgacccgg aggttaccat cttctttggc ctctcggggg ccatgatccc ggcaggcatg
      961 caaaagattc tcatcgaact tgcgaggcgc cattatatcg atgtggtggt ctctacgggt
     1021 gcgaatattt tccacgatgt ctgcgaacac ctgggtgtct gccactacct gggccaccac
     1081 cacgtcagcg acgccgggct gtatgagaag ggaatcgatc gcatctacga cgtctttgcg
     1141 tttgaagagg agttccgatg ggtcgacaga gggtttgcga acttcgctga gaggattgcg
     1201 ccgttcaggg gatcttcaag ggaatttctg cagaggtttg gagagtggct tatcagggaa
     1261 gtgccggacg gcaactcgtt cctcgcagca tgcgtgcggg agaggatacc tgtttttgtc
     1321 ccggctctct gcgattcatc gatcgggatc ggtgcactca ttgcgaggcg ccgaggcgtc
     1381 gagatcgaca tcgaccagat tgccgatacg gacgaggtca cgtcccttgt ggaagagggc
     1441 agaaagaccg gggtggtcta cgtgggtggc ggggtcccga agaacttcat ccagcagacc
     1501 caggttatcg gttctatcca cgagaaggac cttggcggcc atgattatgc gatccagtac
     1561 accgccggct caccgcactg ggggggtctg tcaggctgca ccttcgaaga ggccatcagc
     1621 tgggggaagg agtcagcggc gaccaggcac gtccaggtgc acgtggatgc cacgattgcg
     1681 ctcccaatcg tcgtatccgc gctcatcggg agcggcgtgc caagggtcgg caggaagggt
     1741 gcaaagagga gcaaggtact caggcagggg gtcgattagt aaaattccaa gatgacctga
     1801 tcaggtactt ttttatctcg aaatcgtcac acgctttccc acacaacaat ggacctccca
     1861 tatctccgtc accgtattct ctatgtcgac gatgaacccg cccttctgga catctgcagg
     1921 ttattcctgg aaaaatcagg acattttgaa gttgttacag taaactcggg gtttcttgcc
     1981 cttgattgcc tggcccatca gcattttgat gcggtagtct ctgactacca gatgcctggc
     2041 atggatggta ttgaactcct ccgcagggtc aggcgggagg gaaacacgat ccctttcatt
     2101 atcttcacag gcaaaggcag ggaggaggtg gtcatccagg cgttgaacga gggggctgac
     2161 cactatctgc agaaaggagg ccagccgaag gcacagtttg tcgagcttgc ccacaaggtg
     2221 aagacatcta tcttaaaaaa agaggccgaa gctgcgctga aagagagcga agagaagtac
     2281 cggatgctcg tcgagatcaa caggga
//
LOCUS       DAOQVJ010000268         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_58214_length_2293_cov_4.223861, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000268 DAOQVJ010000000
VERSION     DAOQVJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58214_length_2293_cov_4.223861"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1885
                     /locus_tag="PKL26_06115"
     CDS             131..1885
                     /locus_tag="PKL26_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNS83303.1"
                     /translation="MIAIVVGVVSGFGSLLFFEGLTIATQFFMGTCLGYSYPVEGQSA
                     AEIAGWAPPPNIWVLLPIICFGALASGILVTKLAPETEGHGTDAAIRAFHGEGTIRKR
                     VPFLKAITAILTISTGGSAGREGPTAQIAAGIGSMIADMLKLPLHERRMALNIGIGAG
                     IGTIFLAPLGGAILAAEILYRRDFESEVIVPAFLASIIGYSIFGLFEGFSPVFAEAPI
                     VWNVFQIPFFLVLGLICAGYGWLYIHTFYRSHEVFADLVRKYHFPPFIKPVIGAFLTG
                     LIVIILFLFIPNGAEIGLGSLGSGYGFVQLALYSMLPLGVLVALSFLKILTTSLTIGS
                     GGSGGVFAPGLVIGGMVGGMCGVIFNILFPAYFPVETIPAFVIIGMIALFGCVSYAPI
                     AVMIMVVEMTGNFSLLVPAMGAVAIACLFTRGSGIYRAQVETKADSGAHRGEYEVSIL
                     QKIPAGTSMTPAEKVLVLHPEDKSREVLHLIRLHGHNGFPVIDNGKLVGIMTIQDVRQ
                     VRKKQDLDAPVKDGMSRDLFTLTPDTTLDNALKIMIERDINHIPIVDTIESQKLVGFL
                     TRTDIMNAYARYSEENSL"
     gene            2006..>2293
                     /locus_tag="PKL26_06120"
     CDS             2006..>2293
                     /locus_tag="PKL26_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83304.1"
                     /translation="MNRIIHFEIQADEPEQVAKFYRSVFGWEINEWVLPGFEMKDEDR
                     YWIVITGAEGEPGINGGLVFRHGPRAPGRLSANAFFCTIGVADLDMSVKKVT"
BASE COUNT          517 a          560 c          597 g          619 t
ORIGIN      
        1 ccttgctcat atctttcctt ggttgttgat ccatattccg acccccaatt taaatcgaaa
       61 tgtaattaat ttatgggatt gttagtacga taacatgaac tacggtggca ctacctttca
      121 gcgattactc atgatcgcca tcgtggtagg agtggtatcc ggttttgggt cgcttctttt
      181 cttcgagggt ctcactatcg caacccagtt ttttatgggt acgtgcctcg gatattcata
      241 ccctgttgaa ggacaatccg cagccgaaat tgcaggttgg gctccgccac ccaatatctg
      301 ggtccttctc ccaatcatct gctttggagc gctcgcttcc ggaatacttg tcacaaaact
      361 tgctcctgag accgaggggc atggtaccga tgccgccatc agggccttcc atggcgaggg
      421 aaccatacgg aagcgcgtcc cttttcttaa agcgatcacc gccatcctga ccatatcgac
      481 cggtggtagt gcaggaagag aggggcctac agcccagatt gcggccggca tagggtcgat
      541 gatcgctgat atgctcaaat taccactcca tgagcgaaga atggctctta atattggaat
      601 aggggcagga attggcacaa ttttcctggc acctcttggc ggcgctatcc ttgctgctga
      661 gatcctttat cgaagagact ttgaatcaga agttatcgtc cccgcgttcc tcgcctctat
      721 catcggctat tcaatattcg gactcttcga ggggttcagt ccggtgtttg cagaagctcc
      781 gattgtatgg aacgtgtttc agattccatt tttccttgtc cttggcctga tatgcgccgg
      841 gtatgggtgg ctatacattc atacctttta caggagccat gaggtatttg cagaccttgt
      901 gaggaagtat cattttccac cgtttataaa gcccgttata ggcgcttttc ttaccggtct
      961 aatcgtcatt atcctctttc tcttcatccc caacggtgca gagatcggcc tgggcagcct
     1021 ggggtcaggg tatgggttcg tccagcttgc gctctattcg atgcttcccc tcggggttct
     1081 cgtggctctc tcgttcctga agattcttac gacctcactc accattggtt caggtggcag
     1141 cgggggtgtc tttgcaccgg gtctcgtgat tgggggaatg gttgggggaa tgtgcggagt
     1201 gatattcaac atccttttcc cggcctattt cccggtcgag accatccctg catttgtcat
     1261 aatcgggatg atcgcactct ttggatgcgt atcatatgcg cccatcgcgg tgatgatcat
     1321 ggtcgtcgag atgaccggaa acttttcttt gctggtcccc gcaatgggcg cagtcgcaat
     1381 cgcatgtctg ttcacgaggg gcagcgggat ataccgtgca caggttgaga caaaagcaga
     1441 ttccggggcc catcgcggag aatacgaggt cagcatcctt caaaaaatcc ctgcagggac
     1501 cagcatgact ccggctgaga aggtccttgt acttcatccc gaggataaga gcagggaagt
     1561 ccttcatctc atccgtttac acgggcataa cggatttccg gtcatcgata atgggaagct
     1621 ggtcgggatc atgaccatcc aggacgtgcg gcaggtaagg aaaaagcagg acctggatgc
     1681 accagtgaag gatggaatga gcagggatct ttttactctc acgccagata ccacgcttga
     1741 taacgcactt aagatcatga ttgagcgtga tatcaatcat attccgattg ttgatacgat
     1801 tgaatcacag aaactggttg gtttcctgac ccgcacggat atcatgaatg cgtatgccag
     1861 gtattcggaa gagaattcgc tttgatgcgg ggattattgc agattaccgg ataccttcat
     1921 tacacctttt tgtaattttt tttagaaagg tgtcattgta actaataagg acgtccaacc
     1981 agagagtaag tctaagagaa tattcatgaa ccggatcatc cattttgaaa ttcaggctga
     2041 tgaacctgaa caggttgcga agttctacag atccgtattc ggatgggaga tcaacgaatg
     2101 ggttcttccc ggatttgaga tgaaggacga ggaccggtac tggattgtga tcaccggggc
     2161 tgaaggcgaa cccggcatca acgggggcct cgtattccgc catggaccca gggctcccgg
     2221 cagactgtcg gcgaacgcgt tcttctgcac gatcggtgtt gccgacctcg acatgtccgt
     2281 caagaaagtg acc
//
LOCUS       DAOQVJ010000269         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_58217_length_2293_cov_4.048704, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000269 DAOQVJ010000000
VERSION     DAOQVJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58217_length_2293_cov_4.048704"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PKL26_06125"
     CDS             complement(<1..1400)
                     /locus_tag="PKL26_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNS83305.1"
                     /translation="MTDPDAGERDSSNPANRLVHEASPYLQQHAHNPVDWYPWGEEAF
                     SRARAEGKPIFLSIGYSTCHWCHVMERESFSDREVAALLNAHFICIKVDREERPDLDQ
                     LYMDAAIALTGSGGWPLNVVLTPDLHPFFAATYLPRENRMGMPGLLEVLPRLAEFWEE
                     KQEEALESAGLLARAIAADRKARPGGRVRKDTADRMLQDLLLQYDSLNGGFGPAPKFP
                     MPHLHLFLLRYWKWTGSGKALGMAEKTLLSMARGGIYDHLGGGFHRYSTDARWLVPHF
                     EKMLYDQALAAMAFTEAWLATGNQEFWEVASGCLDYACSRLSAPDGGFFSAEDADSEG
                     VEGKYYLWTHDEIASLLDPEDARVAFLVFPVSRTGNYLDPHTGRYGRENVLHRTHSEK
                     EAARALSLSPGEVSERIERARATLLRAREMRTRPFCDEKVLADWNGLAIAALAMAGRA
                     SGRSRYIEASKKAATFV"
     gene            1643..>2293
                     /locus_tag="PKL26_06130"
     CDS             1643..>2293
                     /locus_tag="PKL26_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNS83306.1"
                     /translation="MEKGSRSGGAEAESRLSPAMAQYQEIKSKYPDAILLFHIGDFYE
                     TFNEDAETVSRELDIVLTSRSRDRSGNRIPLAGVPCHAVEGYIARLIAKGYKVAVCDQ
                     VEDARAAKGIVKREVVRVVTPGMVMDESLIPSPGAHYLLAVSPDAKEGEAGLAFLDIT
                     TGEFSVVTVPLEHGFSALQGEVARNAPAECLLPGDAPPELAESLSRGGLAVSRGAVD"
BASE COUNT          439 a          701 c          711 g          442 t
ORIGIN      
        1 acaaaggttg cagccttttt ggatgcttcg atatatctgc tcctgccaga tgccctcccg
       61 gccatggcga gcgcggcaat cgcaagtccg ttccagtcgg caaggacctt ctcatcgcaa
      121 aaaggccttg ttcgcatctc acgggcccgg agcagggtgg cccttgccct ctcaatccgc
      181 tcgctgacct ctccgggcga aagagagagg gcccgtgccg cctctttttc ggaatgggtg
      241 cggtggagga cgttctccct cccatagcgg ccggtgtgcg ggtcaagata atttcctgtc
      301 cggctgacag ggaagaccag gaaagccacc cgcgcatctt ccgggtcgag gagggatgcg
      361 atctcgtcat gcgtccagag gtaatacttc ccttccacgc cctcgctgtc ggcatcctct
      421 gccgagaaga accccccgtc cggggcggag agccgggagc aggcatagtc caggcagccg
      481 gatgcaacct cccaaaactc ctggtttccc gtcgcgagcc atgcctcagt gaaggccatc
      541 gcggccaggg cctggtcata gagcatcttc tcgaagtgcg ggaccagcca ccgtgcatca
      601 gtcgagtacc ggtgaaatcc cccgccgagg tggtcgtata tgccgcccct tgccatggag
      661 aggagggtct tttcggccat gccaagggct ttgccgctcc cggtccactt ccagtagcgg
      721 agcaggaaga ggtggaggtg cggcatcggg aacttgggcg caggaccgaa cccaccgttc
      781 aggctgtcgt attggaggag aaggtcctgg agcatccggt cagctgtgtc ctttctcacc
      841 cggccgccgg gtctcgcctt gcgatcggca gcgatggcgc gtgcgaggag ccctgcggac
      901 tccagcgcct cttcctgctt ctcctcccag aactctgcga gccttgggag gacctccagt
      961 agtcccggca tccccatcct gttctccctc gggagatatg tcgccgcaaa aaacgggtgg
     1021 agatcagggg tgaggacgac attgaggggc cagccgcccg agcccgtgag ggcgatcgcg
     1081 gcatccatgt agagctggtc gagatccggc cgctcctcgc gatccacctt gatgcagatg
     1141 aaatgtgcat tgaggagggc ggcaacctcg cggtcagaga aagactccct ctccatcacg
     1201 tggcaccagt ggcaggtgga gtatccgatg gagaggaaga tcggctttcc ttcagcccgc
     1261 gccctggaaa acgcctcctc tccccacggg taccagtcaa cggggttatg cgcgtgctgc
     1321 tgcaggtagg gtgacgcctc gtggacgagc cggttcgccg gattcgaaga atccctctct
     1381 ccagcgtccg gatcagtcac cggcaccgct ccctggcctc agggactctc tctttcgctc
     1441 ttcatccagt ggctctctcc ttgttgcccg gaaagcagga aagaagcaga cagcggcgta
     1501 gaggaacaca agacccacac cggaggatat ggaaaaaggg acacttgaat attcgcacca
     1561 tggcaggaag gggagactct cccagtagcc ctgagcccgc gatcgttaca cataactctc
     1621 cctatgcacg agagtatgag tgatggaaaa gggatcgcgg tccgggggag ccgaggcgga
     1681 gagccgcctc tcccctgcca tggcccagta ccaggagatt aagtcaaaat atcccgatgc
     1741 tatccttctt tttcatatcg gagattttta cgagacgttc aacgaggatg ccgagaccgt
     1801 gtccagggag cttgacatag tcctcacctc ccgttccagg gaccgcagcg ggaacaggat
     1861 tcctcttgcg ggggtgccct gccatgcggt tgaagggtac atcgcgaggc tgatcgcaaa
     1921 aggctacaag gtcgcggtct gcgaccaggt ggaggatgca cgggctgcga agggaattgt
     1981 aaagagggag gtggtccgtg tcgtgacccc cggcatggtg atggacgagt ccctcatccc
     2041 ctctccgggt gcccactacc tcctcgctgt ctctcctgac gcaaaggaag gagaggcggg
     2101 acttgcgttc ctcgacatca ccaccgggga gttctcggtc gtcacggttc ccctcgaaca
     2161 cgggttttcc gcgctccagg gggaggttgc gaggaatgct cctgccgagt gccttctgcc
     2221 aggcgacgct cccccggaac tcgcagagtc cctttcgagg ggcggccttg cggtgagccg
     2281 tggagcggta gat
//
LOCUS       DAOQVJ010000270         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_58385_length_2289_cov_4.458818, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000270 DAOQVJ010000000
VERSION     DAOQVJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58385_length_2289_cov_4.458818"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKL26_06135"
     CDS             complement(<1..661)
                     /locus_tag="PKL26_06135"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNS83307.1"
                     /translation="MGRFAIVDDERIKKGECTDIYFSRSEAVLSASGNNPIVAMEVTA
                     SAVPGGMGILCGMEDVLSLLEDLPLDAYAMPEGSLFHQWEPVLRIEGNYRDIARYETA
                     ILGFLCHASGIATAAARIKAAAAERMVFSFGSRRQHPAIATMVERSAWIGGVDGVSNT
                     CAPAGIPLAGTMPHAFVMCYSSPAEAFQAFDRHSPPEVSRIMLCDTFCDEKRESLTGA
                     RC"
     gene            800..1471
                     /locus_tag="PKL26_06140"
     CDS             800..1471
                     /locus_tag="PKL26_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HNS83308.1"
                     /translation="MLGIIGGTSLLFARLPDLEKRVVHTPYGNSEVMAGEVALLLRHQ
                     RDIPPHRINYRSHLSALALLGVDRLVVLGSSGSLKKEIPPGSMVIPTDYFSSAAVPTL
                     HDHAICHVHPGFSPELSNHLKEIVPEAIFGGTYVQTRGPRIETVAEVRALALCGDIVG
                     MTLASEATLAVELGIEVAALCTIDNYANGLSDELLTYERIVEYSARYKDRTEEIISRI
                     IRELA"
     gene            1483..>2289
                     /locus_tag="PKL26_06145"
     CDS             1483..>2289
                     /locus_tag="PKL26_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS83309.1"
                     /translation="MKNTQTKGTSLLIERVLVNGTLQDIFVDETGVITSIGEGIARTF
                     RGEAETCIDGAGDLALSGLVNTHTHAAMTLLRGFADDMPLQDWLTQKIWPLEAHLTGE
                     DVYWGTKLACLEMIRSGTVAFNDMYFFMDEAARAVDEMGLCAVLAYGFLDLFSEEKRE
                     AECRATERLVQSIHGMKNPRIKAAVGPHAMYTVSPEGLRWVSEFAAEKEIGIHVHLSE
                     TEKEVTDAVSQWQKRPVKILDECGILTPRTVAAHCCWLDRDECSFVAKRGV"
BASE COUNT          484 a          672 c          713 g          420 t
ORIGIN      
        1 cgcagcgggc accggtgaga gactccctct tctcatcgca gaaggtgtcg cagagcatga
       61 tgcgggagac ttccggcggg gaatggcgat cgaacgcctg gaatgcctcg gccggcgatg
      121 aatagcacat tacgaatgca tgcggcatgg ttcctgcaag aggaattcca gcgggggcac
      181 aggtgttgct caccccgtcg acgccgccaa tccacgccga gcgctcgacc atcgtggcga
      241 tagcggggtg ctgccggcgt gacccgaagg agaacaccat ccgttccgct gcagctgcct
      301 tgatcctggc ggcggcggtg gcgatgcctg atgcatggca gaggaacccg aggattgccg
      361 tttcataccg ggcaatatcg cggtagttcc cctcgatgcg aagcaccggc tcccattgat
      421 gaaagagcga gccttccggc atggcatacg catcaagggg gagatcttca aggagggata
      481 gcacgtcttc catcccgcag aggatgccca tgcccccagg gaccgcggac gccgtgacct
      541 ccatggcaac gattggattg ttgcccgaag cagagaggac cgcttcgctc cttgagaaat
      601 agatgtccgt gcactctccc ttttttatcc gttcgtcatc gacaatggca aacctgccca
      661 tggatgtatt gtgggagcgg tgaaaagata caggtattga ctccccgcgc agagtcgcac
      721 cgaaagccag aacaaccgcc cgttccagat tacttaagga caagatcatg aaccgcacga
      781 ttttcacggc ggtggcctga tgctcgggat catcgggggc acaagccttc tctttgcgag
      841 gcttcccgac ctggagaaga gggtggtgca cacgccgtat ggcaacagcg aggtgatggc
      901 aggagaagtc gccctcctcc tccggcacca gcgggacatc ccgccgcacc ggatcaacta
      961 ccggtcgcac ctctcggccc ttgcgctcct cggcgtggat cgcctggtgg tgctcgggtc
     1021 gtcgggatcg ctcaaaaaag agatcccgcc ggggagcatg gtgatcccga ccgattactt
     1081 ctcctctgca gccgtcccaa cccttcacga ccatgccata tgccacgtgc accctgggtt
     1141 ctctccggag ttgtcaaacc atctgaagga gattgtcccc gaggcaattt tcggggggac
     1201 ttacgtccag acaaggggcc cgaggattga gacggtggcc gaggtcaggg cgcttgccct
     1261 ctgcggcgat atcgtgggga tgacgcttgc aagcgaggcg acccttgcag tcgaactcgg
     1321 gatcgaggtt gcggcgctct gcaccatcga caactacgca aacggcctct cggacgaact
     1381 gctcacttac gagaggatcg tggaatactc ggcgcggtat aaagatcgga cggaggagat
     1441 tatctcccgc atcatccggg aactcgccta ggagattaca tcatgaagaa tacgcaaaca
     1501 aaaggaactt cgctcctgat cgagcgggtc ttggtgaacg gaacgctcca ggatatcttc
     1561 gtggacgaga ctggggttat cacttcgata ggggagggaa tcgcacgcac ctttcgcggt
     1621 gaagcggaga cctgcatcga cggtgccggc gacctggcgt tgtcgggcct cgtgaacacc
     1681 cacacccacg ccgccatgac cctgctccgg ggctttgccg acgacatgcc gctgcaggat
     1741 tggctgaccc agaagatctg gcctctcgag gcccacctta ccggggagga cgtatactgg
     1801 ggcacgaaac tcgcgtgcct cgagatgatc aggtccggca ccgtggcctt caatgacatg
     1861 tacttcttca tggatgaggc cgcccgggcg gttgacgaga tggggctctg tgcggtcctc
     1921 gcctatggat tcctcgacct cttctcggag gagaagcgcg aggccgagtg ccgggcgacc
     1981 gagcgcctgg tgcaatcgat acacggcatg aaaaatccca ggatcaaagc agccgtcggg
     2041 ccgcacgcaa tgtacaccgt ctcgcccgaa ggactgaggt gggtctcgga attcgctgcc
     2101 gaaaaggaga tcgggatcca cgtccactta agcgagacgg aaaaggaggt aacagacgcg
     2161 gtctcccagt ggcagaagcg cccggtgaag atcctcgatg aatgcggcat actgacaccc
     2221 cggacggtgg ccgcccactg ctgctggctt gacagggacg agtgcagctt cgttgcgaaa
     2281 cggggggtg
//
LOCUS       DAOQVJ010000271         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59069_length_2272_cov_4.003157, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000271 DAOQVJ010000000
VERSION     DAOQVJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59069_length_2272_cov_4.003157"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..404
                     /locus_tag="PKL26_06150"
     CDS             150..404
                     /locus_tag="PKL26_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNS83310.1"
                     /translation="MIDLVIHALVIAGLVASALMVFHFRDLLAAALAAGFFSFLISLE
                     FYILQAPDVAISQAAIGAGLTTAIFIIAIRATTRYEGGQP"
     gene            401..676
                     /locus_tag="PKL26_06155"
     CDS             401..676
                     /locus_tag="PKL26_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83311.1"
                     /translation="MRKILTAIIFVVLATGLCAIPAFLAFGEPAASEMDQYFIGHGQS
                     EAAANNIVTSIVFDYRGFDTLGESVVLFTGGVGVGLMFRSMKGGKEQ"
     gene            673..1140
                     /locus_tag="PKL26_06160"
     CDS             673..1140
                     /locus_tag="PKL26_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNS83312.1"
                     /translation="MRMSKIVRTGANLLYPFILIFGLYVIMHGHLTPGGGFQGGAVVA
                     TAAALVIVAYSYEEVTGMLKKTAMKARESIGLLLFIGAGIAALAVGAPFFYNFLANSG
                     LLFGMPVPAGPNPGDLNTAGVIPIMNIAVGIEVWGGLAVILLYMLSGIRKEES"
     gene            1143..1487
                     /locus_tag="PKL26_06165"
     CDS             1143..1487
                     /locus_tag="PKL26_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HNS83313.1"
                     /translation="MFWNLPLIAVVVLTIIALAIIVFKKNLIKVFLGIVILESAVNLF
                     LISLGYREGGIAPIYTNAPSTAMVLPTPQALTLTSIVIGVATSALLLSFAIIIWKKYG
                     TIDIDRIRRLKE"
     gene            1484..>2272
                     /locus_tag="PKL26_06170"
     CDS             1484..>2272
                     /locus_tag="PKL26_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNS83314.1"
                     /translation="MIPSFIVENSPALMVIVPLVAAFITPFIGRISSGIRSLWVGGAM
                     AVTSAITLILAFDVYSAGTRLYVFGARAASAAIPPDSGGIPIRIIFEVDPMSALMLVI
                     TAVSGFAIVLYSLSSERSHTGLESYYTLYLLMITGILGMVSTGDIFNFFVFLEILSLA
                     SAGLIAYRIDGGVAVEAALKYFVLSTIGALAILVAIGIYYGEYNALNMAMIAARMQFS
                     LLDQVALVLIIAALAMKCGAVPMHFWTPDAYSMAPSSITALLVVA"
BASE COUNT          410 a          700 c          641 g          521 t
ORIGIN      
        1 tatgtgacgc tctctgttca taccctgatc gctgcgatct cccttgcatt cacgaacgcg
       61 gtagggtccc atgccatcgc acgcggcgca cacaaggccg ggataaagcc agctccagca
      121 atcgtggacc ggctggagga gtttacacca tgatcgacct ggtcatccat gccctggtaa
      181 ttgccgggct tgttgcctcc gcgctgatgg tctttcattt ccgcgacctg cttgcggcag
      241 cactcgctgc aggtttcttc agtttcctca tctcgctcga gttctatatc ctgcaggccc
      301 cggacgtggc aatctcgcag gctgccatcg gggccgggct caccactgcc atcttcatca
      361 tcgccatacg ggcaactacg agatacgagg gaggtcagcc atgaggaaga tcctgaccgc
      421 gatcatattc gtggtgctgg caaccgggct ctgtgccatc cctgctttcc tggcattcgg
      481 cgagcccgct gcgtccgaga tggaccagta tttcattggg cacgggcagt cggaggccgc
      541 ggcaaacaac atcgtcacct cgatcgtgtt cgactacagg ggattcgata cgctcgggga
      601 gtctgtcgtc ctcttcacgg ggggggtggg ggtggggctg atgttccgtt caatgaaagg
      661 agggaaagaa caatgaggat gagcaagatc gtgagaacgg gggccaacct tctctatccc
      721 ttcatcctca tcttcggcct ctacgtgatc atgcacggac atctcacgcc tggcgggggg
      781 ttccaggggg gtgcggtggt ggcgaccgcc gcagccctgg tcatcgttgc ctattcttat
      841 gaagaggtga caggcatgct gaagaagacg gcgatgaagg cccgcgagtc gatcgggctc
      901 ctgctcttca ttggtgccgg gatcgctgca ctggcggtgg gcgctccgtt cttctataac
      961 ttccttgcaa acagcggcct cctcttcgga atgccagtcc cggccggccc gaaccccggc
     1021 gatctgaaca ccgcgggagt catccccatc atgaatatcg cagtggggat cgaggtctgg
     1081 ggaggtcttg cggtcatcct cctctacatg ctctccggta tcaggaagga ggagtcctga
     1141 ccatgttctg gaaccttccc ctcatcgcag tggtcgttct gaccatcatt gcacttgcaa
     1201 tcatcgtgtt caagaaaaac cttatcaagg tcttccttgg gatcgttatc ctcgagtccg
     1261 cagtcaatct cttcctcata tcgctcgggt accgcgaggg agggatcgca cccatctaca
     1321 cgaacgcacc atcgactgca atggtgcttc ccacccccca ggccctcacc ctgacctcga
     1381 tcgtgatcgg ggtggccacg agtgccctgc tcctctcgtt tgccataatc atatggaaga
     1441 agtacgggac catcgatatt gacaggatcc ggaggctgaa ggaatgatcc cctccttcat
     1501 tgtggagaac tccccggccc tgatggttat cgtgcccctc gttgcggcct tcatcacccc
     1561 gttcatcggt cgcatatcgt cagggatccg ctcgctctgg gtgggcggtg ccatggccgt
     1621 cacatccgcc atcaccctca tcctggcatt cgatgtgtac tcggcaggga ccaggttgta
     1681 tgtcttcggg gcaagagccg catcggcggc aatccccccc gactcgggcg gcatccccat
     1741 caggatcata ttcgaggtcg acccgatgag cgccctgatg ctcgtgatta ccgcggtatc
     1801 gggctttgcc attgtactct actccctctc gagcgagcgg tcccacacgg gccttgagag
     1861 ctactacaca ctctacctcc ttatgatcac cgggatcctc gggatggtct ccacggggga
     1921 tatcttcaac ttcttcgtct tcctcgagat cctctcgctt gcgtccgcgg ggcttatcgc
     1981 ataccggata gacggcggag tggcggtgga ggcggccctc aagtacttcg tcctgagcac
     2041 gattggggca cttgcaatcc tcgtcgcgat cgggatctac tacggggagt ataatgccct
     2101 gaacatggcc atgatcgccg cgaggatgca gttctccctc cttgaccagg tggcgctggt
     2161 gctgatcatt gccgcgctcg caatgaagtg cggtgcagtc ccgatgcatt tctggacgcc
     2221 cgacgcatac tcgatggcac cttcctccat caccgcactc ctggtggtcg ca
//
LOCUS       DAOQVJ010000272         2269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59208_length_2269_cov_4.319332, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000272 DAOQVJ010000000
VERSION     DAOQVJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59208_length_2269_cov_4.319332"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKL26_06175"
     CDS             complement(<1..456)
                     /locus_tag="PKL26_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039303.1"
                     /note="catalyzes the S-adenosyl-methionine-dependent
                     2'-O-ribose methylation of C56 in tRNA transcripts;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HNS83315.1"
                     /translation="MTEIFVLRIGHRPERDQRVTTHVGLTARALGARGMYLAARDSGV
                     VTSIRDVVSRWGGDFLVQDQVSWKKCIRDWKAAGGIVVHLTMYGEGIRSHEEALQRCH
                     RILVVVGAEKVPGEVYGLADYNISVTGQPHSEIAGLAVFLDRFFKGTELE"
     gene            complement(449..739)
                     /locus_tag="PKL26_06180"
     CDS             complement(449..739)
                     /locus_tag="PKL26_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNS83316.1"
                     /translation="MREEELDWQVYHLLMDDAGRDEDALAALLHCTPGEVHTSIGRLE
                     KAMLLECTPGGVRVLSVQEMALRCQARYDRSCPFSIKGGVIRLKGGSDEKDD"
     gene            954..1364
                     /locus_tag="PKL26_06185"
     CDS             954..1364
                     /locus_tag="PKL26_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83317.1"
                     /translation="MQDREKVPSFGELAGIAEHLLEKADEDEALLARELDTVDAAVRR
                     ELLVSDFLNAYQVYYYYFREAPGELEKERLILQPASALVQGVMIAELELLEVIFRVED
                     DQPVMSVSDGDQVLVNYRGRDAYRRALAFIDASL"
     gene            complement(1443..2057)
                     /locus_tag="PKL26_06190"
     CDS             complement(1443..2057)
                     /locus_tag="PKL26_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS83318.1"
                     /translation="MEGAMDRTILVVDDNRRLVDLLRTILEMEGYIVHAAYSGDECLA
                     YLDRERPGLILLDIVMSPMDGWTVLERIRERPGTGKTRVIMLTARHPTRKEAEKYAAL
                     IDGYILKPFDVRNLLESIRRAFAEQERLEGVIRSAHEKGTKRSFLAEYCRLSRIVNAQ
                     EALRALLSPGIGQGEGEEESDEARKLATMKQIIQQVVGIPGLDE"
BASE COUNT          438 a          725 c          613 g          493 t
ORIGIN      
        1 ctccagttcg gttcccttga agaaacggtc gaggaagacc gcgaggcccg ctatctccga
       61 gtggggctgc ccggtcaccg agatattgta atcggccagg ccatatacct cccccggaac
      121 cttctctgcc cccactacca cgaggattcg gtggcacctc tgcaacgcct cttcgtggct
      181 cctgatcccc tcgccgtaca tcgtgaggtg cacgacaatg cccccggcag ccttccagtc
      241 ccggatgcac ttcttccagg agacctggtc ctgcacgaga aaatcaccac cccaccggga
      301 gaccacgtcg cggatgcttg tgaccacccc ggaatccctc gcggcaaggt acatcccccg
      361 ggccccgagc gcccgcgcag tgagcccgac atgggtggtc accctctggt cgcgctcggg
      421 ccggtgtcca atccggagga cgaatatttc agtcatcttt ttcgtcactt ccgcctttca
      481 agcggatcac tccgccctta atcgagaatg gacaactccg gtcgtagcgg gcctggcacc
      541 gcagtgccat ctcctgtaca gagagcacac ggacccctcc cggcgtgcat tcgaggagca
      601 tggccttctc cagccgcccg atggatgtgt gcacctctcc cggcgtgcaa tgcagaagcg
      661 cggcaagcgc atcctcgtcc ctgcccgcat catccatcag caggtgatat acctgccagt
      721 ccagctcttc ttctctcatg tgctgtcgcg tagcgtaagg tatggaaaaa agaggagata
      781 atgctcgtgg aggaatacgc ggccatcgcg cttttgaggg cgtgggaaac cctcacctct
      841 acagtgcccg catccgggaa tgacatgctg gcccatcttt cggctctcat ccatggaacc
      901 ttacaaagat tgagattcac tatctataag gagccgccgc tccatccctt gatatgcaag
      961 atcgggagaa ggtcccctct tttggggaac ttgcgggaat cgctgagcac ctcctcgaga
     1021 aggcggacga ggacgaggcg cttttagcca gggaactcga taccgtcgac gcagcagtcc
     1081 gccgggagct gctcgtatcc gactttctga acgcatacca ggtctactac tactatttcc
     1141 gggaggcccc gggtgagctg gagaaggagc ggctgatcct ccagcccgca tctgcgctgg
     1201 tgcagggcgt gatgatcgcg gaactcgaac ttctcgaggt catcttccgg gtcgaggacg
     1261 accaaccggt gatgagcgtc agtgacggcg accaggtcct ggtcaattac cgcggcaggg
     1321 atgcataccg gagggcgctt gcgtttattg atgcctcgct ctaggatcgt gcatggatgg
     1381 cgggcctttt cgcggcccca ctgaaaacag atccatggcc tgttaccttg gcaggcagat
     1441 cgttattcat ccaaacccgg tatcccgacc acctgctgga tgatttgctt catcgtcgca
     1501 agtttcctgg cttcatcgct ctcctcttcc ccctctccct ggccgattcc cggcgagagg
     1561 agggctctca atgcctcctg cgcattcacg atacgcgaga gcctgcagta ttccgcaagg
     1621 aaacttcgct tggtgccctt ctcgtgcgcg ctcctgatga cgccttcaag gcgttcctgt
     1681 tctgcaaagg cacgacggat actctccagg aggttgcgca catcgaaggg cttgagtatg
     1741 tacccatcaa taagcgcggc atacttctct gcttcctttc tcgtgggatg cctggcggtc
     1801 agcatgatca cccgggtctt tcccgtgcca ggtctctccc gtatgcgctc gagaaccgtc
     1861 cagccgtcca tgggagacat gacgatgtca aggagaatga ggcctggcct ttctctgtcg
     1921 aggtatgcga ggcactcgtc gccactatag gctgcatgga cgatgtaccc ctccatctcg
     1981 aggatggtcc ggaggagatc aacgagcctg cggttatcgt caacgacgag aatggtcctg
     2041 tccattgcgc cttccattca cccccatcat acccgccgga aattgtgtgc acggtggggt
     2101 cttcccaagg attcacacct ccacgctttc gcgcacctgc acgcgtaaat cttcttagcg
     2161 aaccatgtag tctttttgat cctcaccatg gaccacctgt gacaaggtcg tggtccactc
     2221 ttgccctgac catgtgcgga aggctgccaa acgacgaggc ccattcccc
//
LOCUS       DAOQVJ010000273         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59446_length_2263_cov_3.928442, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000273 DAOQVJ010000000
VERSION     DAOQVJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59446_length_2263_cov_3.928442"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PKL26_06195"
     CDS             complement(<1..153)
                     /locus_tag="PKL26_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase TatD"
                     /protein_id="HNS83319.1"
                     /translation="MGGPRFPITDHHIHLDPRNGRGIDAAKDFRRSGGTHLFLVSKPS
                     SSFGILP"
     gene            complement(154..477)
                     /locus_tag="PKL26_06200"
     CDS             complement(154..477)
                     /locus_tag="PKL26_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HNS83320.1"
                     /translation="MHTAREKAAFEAGIKLGALYHQWVGTPVSPLTAASIESAIEKSV
                     GLQPHVKGISVRLDRDIMKPNVFGYSELQGLMLTVEIETRVDGEVCRARLRREGDYPM
                     MEIVE"
     gene            complement(518..>2263)
                     /locus_tag="PKL26_06205"
     CDS             complement(518..>2263)
                     /locus_tag="PKL26_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HNS83321.1"
                     /translation="RKTTEVRPRIVQAVFRCPGGHITIKEQGYGKFREPDGCATEGCT
                     FKKLDLIARRSRFVDSQKLRIQESPEGLRGGEQPQTLDVDMTDDLTGMVAPGDRVVIN
                     GILRSMQRVTHGEKNTLFDIYLECNSIEIAEKEFEEVQIDEKDEEEILTLSRDPNIYR
                     KIIHSIAPTIYGNEDVKEAIALQIFGGISKEMPDGSHLRGDIHVLLIGDPGIAKSQLL
                     RYVVKLSPRAIYTSGQSSTSAGLTATAVKDEFGDGRWTLEAGALVLADMGIAAVDEMD
                     KMQKEDRSALHEAMEQQSISVAKAGITATLKSRCALLGAANPKLGRFDAYLPIQDQIN
                     MPGSLLSRFDLIFKMTDIPDHTRDSAIAIHILKAHSIGETIAQHKRTPIPGVDDAYIE
                     EQLRPVTPEIEPALFRKYVAYARRTCFPRLGDAAREALIGYYMQLRDLAAGSDKPVPV
                     TARQLEALVRLAEASARIRLSPNIEAEDSERVIKIVDTCLREVAYDAKTGTFDIDKVV
                     TGISKGRRDLIRVIKQTIRQIQKDTGNDRIAVAELIDLLTPQGFSRDEIDAQIKVFLR
                     EGEAMEPKSGFVKLI"
BASE COUNT          388 a          675 c          692 g          508 t
ORIGIN      
        1 gggcaggatg ccaaaggagg aggagggctt gctgaccagg aagagatgcg ttccacccga
       61 acgccggaag tcctttgcag catcgatacc ccgcccgttc cggggatcga ggtggatgtg
      121 atggtccgtg atcgggaacc gcgggccgcc catctactcc acgatctcca tcattgggta
      181 gtcaccctcg cgccggaggc gggcacggca gacctcgccg tcaacgcggg tctcgatctc
      241 gaccgtgagc atgagcccct gcagctcgga gtacccgaac acgttcggct tcatgatatc
      301 ccggtcgagc ctgacggaga tgcccttgac atgaggctgg agcccgacac tcttttctat
      361 cgcggattcg atggatgcgg ccgtgagggg tgaaacaggg gttcctaccc actggtggta
      421 cagggccccg agtttgatcc cggcctcaaa agctgctttt tcccttgcag tgtgcatgga
      481 tgtagttctc ggccgggaag caaaaaagta agggggttca gataagcttg acaaagccgc
      541 tcttcggctc catcgcctcg ccttcgcgga gaaacacctt gatctgggca tcgatctcat
      601 cccgggagaa tccctgcggg gtgaggaggt caatcagttc ggcaactgcg atccggtcat
      661 tcccggtgtc tttctgtatc tggcgtatcg tctgcttgat cactctgatc aggtccctcc
      721 ttcccttcga gattcccgtc accaccttgt cgatgtcaaa cgtgccggtc ttggcgtcgt
      781 aggccacttc acgaaggcac gtgtccacga tcttgatgac acgttccgaa tcttcagcct
      841 cgatgttggg ggagagccgg atccgtgcgc tcgcttccgc gaggcggacc agcgcctcga
      901 gctggcgcgc agtgaccggc acgggcttgt cggagcctgc cgcgaggtca cgcagctgca
      961 tgtagtagcc gatgagcgcc tccctggcag catcaccgag gcgcgggaag catgtccggc
     1021 gggcatatgc cacgtatttc ctgaagagtg ccggttctat ctcaggggtc acaggccgga
     1081 gctgctcctc gatatacgcg tcgtccaccc cgggaattgg agtccttttg tgctgggcga
     1141 tggtctcccc gatgctgtga gccttgagga tgtgtatggc aatcgcgctg tccctcgtgt
     1201 ggtcggggat gtcggtcatc ttgaagatga ggtcaaacct ggagaggagg gacccgggca
     1261 tgttgatctg gtcctggatg gggaggtacg cgtcgaaccg gccaagtttt gggttcgcgg
     1321 cgccgagaag cgcacagcgc gacttgaggg tggcggtgat cccggccttc gcgacgctga
     1381 tggactgctg ctccatggcc tcgtgcagcg cggaccggtc ctccttctgc atcttgtcca
     1441 tctcgtccac ggccgcgatt cccatgtccg caagcaccag ggccccggcc tcaagcgtcc
     1501 atctcccgtc cccgaactcg tcctttaccg cggtcgcagt aaggccagcc gacgtggagg
     1561 actggccgct cgtgtaaatt gccctgggag agagcttgac gacataccgg aggagctggc
     1621 tctttgcaat ccccgggtcc ccgatgagga gaacgtggat gtctccgcgg aggtggcttc
     1681 cgtcgggcat ctccttgctt atacccccga agatctggag tgcaattgcc tctttcacgt
     1741 cctcgttccc atagatggtg ggagcgatgg agtggatgat cttccggtag atgtttggat
     1801 cgcgggacag ggtgaggatc tcctcctcgt ctttttcgtc gatctgcacc tcctcgaact
     1861 ctttttcggc aatctcgatg gagttgcatt cgaggtatat gtcaaagagg gtgttcttct
     1921 caccgtgggt gacccgctgc attgatcgga gaatcccgtt gatgaccacc cggtcgcccg
     1981 gtgcgaccat tcctgtcagg tcgtcggtca tgtccacgtc aagggtctgg ggctgctcgc
     2041 cacctctgag accctctggg gactcctgga ttcggagttt ctgcgaatcc acgaaacgtg
     2101 accgtcttgc gataagatca agcttcttga aggtgcatcc ctctgtcgcg cacccgtcag
     2161 gctccctgaa cttgccatac ccctgctcct tgatagtgat gtgccctccc gggcacctga
     2221 agaccgcctg cacgatccgg gggcggacct cggtcgtctt cct
//
LOCUS       DAOQVJ010000274         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59489_length_2262_cov_5.514726, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000274 DAOQVJ010000000
VERSION     DAOQVJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59489_length_2262_cov_5.514726"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..488
                     /locus_tag="PKL26_06210"
     CDS             87..488
                     /locus_tag="PKL26_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNS83322.1"
                     /translation="MALALYGRGVLSSGNAAALAGMTRWQWEELLGEREDSAPLYRRR
                     PRHRQCLWIVTVSNSSPLIHLSQIGRIDLLRCFSSIMVPSAVWREVVEEGDMRPGVKE
                     IRKLHDEGVIIYHRTIQFRPRPAFRAGSSQR"
     gene            complement(638..1438)
                     /locus_tag="PKL26_06215"
     CDS             complement(638..1438)
                     /locus_tag="PKL26_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNS83323.1"
                     /translation="MADPELLRYLSARTGLGVKYLSKDEKISIALEQLGVLIPEAVLK
                     GGTALNRVFLAKSGASRFSEDIDLDFISDDPLPAKIESIKEKMQGVEGFDLDIPRLLH
                     RTLRFDCRYTNEFGDRDRIMVEFFLTKMPFLRVDRVLVASPFIETHPTIFPVYSREDL
                     LARKLVALYSRGEGKDIYDLAYSLDQEYDRAALSKAIEMTCTFYQIEYASFPAALAEK
                     MNALRKNARYIGNSTNHFIPVGRRPDWKTFIDSLARKLETTLVSADIL"
     gene            complement(1425..2219)
                     /locus_tag="PKL26_06220"
     CDS             complement(1425..2219)
                     /locus_tag="PKL26_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83324.1"
                     /translation="MKKKIYLTPEEQEVFRVLSSMQRVESGTVREIFPEKSPHQVNRV
                     LSRLSGKGYLHRVKKGVYLVSPHPSDTGEIRDPYALALALYQGYIGFSSALRIYDLLE
                     YEPFTIFVVTKNTSKEETIGQYLFKAVAMGDRCTGMTWHKGAYVSLLSKTFFDCFYKP
                     QYAGGYAIITRAIYERQPDWNEFCEWFVHESASLCQKTGYILDMLGTRTGIVPEEVTT
                     FFRSRIRNNTRLTGEGTGHSRYIREWKVLDTLGEDHILSWWSHGRS"
BASE COUNT          484 a          590 c          646 g          542 t
ORIGIN      
        1 gatatcacga ttattgtccc gcaggatatc gtccaggcac tccgtcttcc tccagattct
       61 cttcagacaa aattgagaac gcgagcgtgg ctcttgcgct gtatggccgg ggcgtcctct
      121 cttctgggaa cgcagctgca ctcgcaggca tgacccgctg gcaatgggaa gagttgcttg
      181 gggaacgaga agattccgcg ccattatacc gaagaagacc tcgccatcga cagtgcctat
      241 ggatcgtaac ggtcagtaat tcttctccgc tcatccatct ctcccagatc ggtcggatcg
      301 atcttctccg atgtttttca tcgatcatgg taccttctgc ggtctggcgg gaagtcgtgg
      361 aagaggggga tatgcgccct ggagtgaagg agatacggaa attgcatgat gagggggtaa
      421 tcatctatca tagaaccatc caattccgcc ctcgtccagc ttttcgagcg ggatcttcac
      481 agcggtgagg cagagagtta tgcacttgcg ctcgaagtca ggccggatat ccttctttgt
      541 tgtcggaggc gcggcgggat gtcggggcaa tgcgggagat tctctcgagg accgtgggaa
      601 gggtgattcg tggcggaatg ggaattctat caggggatca taaaatatcc gcagagacca
      661 gggtagtctc aagctttcgg gcaagcgaat cgatgaatgt cttccagtcg ggtcttctcc
      721 cgacaggaat gaaatggttg gtggaatttc cgatatatct cgcgttcttc cgcagggcgt
      781 tcatcttttc tgcaagtgca gcgggaaagg acgcgtactc gatctggtag aatgtgcacg
      841 tcatctcgat ggccttggag agtgctgcac ggtcatattc ctggtccagg ctgtaggcaa
      901 ggtcgtagat gtccttcccc tcgccccggg aatacagggc aaccagcttc ctggccagca
      961 ggtcttcccg cgaatagacc ggaaagatgg tggggtgggt ctcgatgaag ggcgaggcga
     1021 ccagaacccg gtcgacccgc aggaagggca tttttgtcag gaagaattca accatgatcc
     1081 ggtcacgatc cccgaattca ttcgtatagc ggcagtcgaa gcgaagtgtc ctgtgcagga
     1141 ggcgggggat atcgaggtcg aatccctcca ccccctgcat cttctctttg atcgattcaa
     1201 tctttgcggg aagcgggtca tctgagatga aatcaaggtc gatatcttct gaaaacctgc
     1261 tggcgccgga cttcgcaagg aagacacggt tcagtgcagt ccctcctttc aggactgcct
     1321 cgggaatgag gactcccagt tgttcgaggg caatcgagat cttctcgtcc ttggagaggt
     1381 atttcacgcc aagacctgtc ctggcagaga gataccgcag cagttcagga tctgccatgc
     1441 gaccaccagg agaggatgtg atcttcaccg agcgtatcga ggactttcca ttccctgata
     1501 taccgcgagt gccccgtacc ctctcccgta aggcgggtgt tattcctgat ccgtgaccgg
     1561 aagaacgtgg ttacttcttc gggaacgatg ccggtacgcg ttcccagcat gtcgaggatg
     1621 taccctgttt tctggcacag ggatgctgat tcatggacaa accattcgca gaactcgttc
     1681 cagtccggct ggcgttcgta gattgcccgg gtgatgatcg cataccctcc cgcgtactgc
     1741 ggtttataaa agcagtcaaa gaacgtcttc gagaggagtg agacgtaggc ccctttatgc
     1801 caggtcattc ccgtgcaccg gtctcccatc gccacggcct tgaacaggta ctgtccgatt
     1861 gtctcttctt tcgaggtgtt tttcgtcacg acgaagatgg tgaagggttc gtattccagg
     1921 agatcgtaga tcctgagcgc ggacgagaac ccgatatatc cttgataaag ggcaagggca
     1981 agggcatacg ggtcgcggat ctccccggtg tccgatggat gcggcgagac caggtatact
     2041 cccttcttca cccggtgaag atagcctttc cccgagagcc gggagaggac cctgttgacc
     2101 tggtgagggc tcttctcggg aaagatctcg cggaccgtcc ccgactcgac ccgctgcatc
     2161 gaggagagca cccggaacac ctcctgctcc tccggggtaa ggtagatctt tttcttcaca
     2221 ttctattctt attgatcaat atctgaataa atttttctac gt
//
LOCUS       DAOQVJ010000275         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59550_length_2261_cov_3.413871, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000275 DAOQVJ010000000
VERSION     DAOQVJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59550_length_2261_cov_3.413871"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1380
                     /locus_tag="PKL26_06225"
     CDS             106..1380
                     /locus_tag="PKL26_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83325.1"
                     /translation="MIVCDVLQRQDEATRARICDAMRYRASNLGRCLLRAREVKEAYF
                     DMPEFKEKRLLAKGTVDIESHDGDIDYMDVRLTMGQLRALIEETPLLYIEESAHSWDI
                     PKDLLFFPCPDYSHLPYKDALMRFSEKVRGQMEQKREAGKISKVLLTGGAARMDLVKT
                     IVESSFPDVIHARMSAPETSVAEGLALRGRAEVMCARFTHDMRNQFSTGLTAMLQEET
                     LRFYHLTSDSVVDAAIREVIDVEIQKACNNRMQGSDLATSCAREVGYWIDDGNLKIHV
                     ISAAEEWFTTSTIDKVNDSLNTLAAKHSIPAVTIGVATALSMARSIETTFIENSLKRG
                     LKGPDVTRAVYESMDIIDKIINLLGFWDLGSLSPIQRGKLGDEIRSRLHKGLMEALQG
                     RSGLARKLQTSLQSDIERGVERIIALETCAYL"
     gene            1666..>2261
                     /locus_tag="PKL26_06230"
     CDS             1666..>2261
                     /locus_tag="PKL26_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83326.1"
                     /translation="MRSILSFCHGISDLRLFFVVNYDGTNYGVYIGVQVQGDAPRTHY
                     LIGNLSQFIQSTWPGTLVEKCGTYRLNDESNTLWNDIGKPIDESTHCLTLTGIPSLRD
                     DNSSGQSQSLDLFMQGMQGQPYTYMVVADSVACGELDNIIAQCRALIGQVHSFKEITL
                     NETLTLARTWGSSVDASESWTDGTSTEDSSMKGWSVGLG"
BASE COUNT          543 a          582 c          652 g          484 t
ORIGIN      
        1 ctcgatctcg gctcatccac tgtcgattcc acgctgctgc agagcgggca ggatctccac
       61 gataaggacg acggatgcga tctcggcgct ttcctgctgg atgagttgat cgtctgtgat
      121 gtccttcaga gacaggatga agcgacacgg gccaggatct gcgacgctat gcgttaccgg
      181 gcaagcaacc tcgggcgctg tctgctgaga gcccgcgaag taaaagaagc ctacttcgat
      241 atgccggagt tcaaggagaa acgtctgctg gcaaagggta cggtagacat cgagtctcat
      301 gacggcgata tcgattacat ggatgttcgg ctgacaatgg gccagctcag ggcgctgatc
      361 gaagagaccc cccttctcta tattgaggag tctgctcatt cctgggatat accaaaggat
      421 ctgctcttct tcccgtgccc tgattatagc catctccctt acaaagatgc gttgatgagg
      481 ttctcagaga aggtaagggg gcagatggaa cagaagcggg aggccggaaa aatctcaaaa
      541 gttcttctta ccgggggcgc cgcccgtatg gacctggtca agacgatcgt cgagtcttcc
      601 ttcccggatg taatccatgc tcgaatgagc gcacctgaga ccagtgttgc tgaaggtctg
      661 gcgctccggg ggagggctga agtcatgtgc gcccgcttca cccatgatat gcgcaaccag
      721 ttcagcaccg ggctcacggc gatgctccag gaggagaccc tccggttcta tcatcttaca
      781 tcagattcgg tcgtcgatgc tgccattcgc gaagttatcg atgtggagat ccagaaggca
      841 tgcaataacc ggatgcaggg cagtgatctg gcgacatcct gcgccagaga ggtgggttac
      901 tggatcgatg atggaaatct taagattcat gtgatatctg cagctgaaga gtggtttacc
      961 acaagcacca tcgataaggt gaatgattcg ctcaacacat tggctgccaa acattctatc
     1021 ccggcagtaa ccatcggggt ggcaacggcc ttaagtatgg cgcgatcgat cgagaccacc
     1081 tttatcgaga atagcctcaa gagggggctt aaggggcctg atgttacccg cgccgtatat
     1141 gagagcatgg atatcatcga caagatcatc aatctgctgg ggttctggga tctggggagc
     1201 ctgagtccca tccagcgggg aaaattggga gacgagatcc gttccagact ccacaaaggg
     1261 ttgatggagg cattacaagg gcggagcggc cttgcgagga aacttcaaac ttcgcttcag
     1321 agcgacatcg agcggggtgt cgagagaata atcgccctgg aaacctgcgc ttatctatga
     1381 gcgagagact ggaaggtatc tcgaaagatc atgggaggta ggaaaatgag agatctatcc
     1441 tcacagaaac ggattgaact ccagcgtatg acggccgatg ccgtgtcgtt gatgctcaac
     1501 ggagttctat cacgccgata tctggaggcg caatggccga acgagaacta cacctcttcg
     1561 atgcccggca cggcatcacc gggtgaactt gctgcctcga tcaaatttct ccggttaacc
     1621 caggtcgggt tgccgatcga agataagaat atgccgatgg aggccatgcg gtcaattcta
     1681 tcgttctgcc atggcatctc ggatctcagg ttgttctttg tcgtcaatta tgacgggaca
     1741 aattatgggg tgtatatcgg ggtacaggtg cagggcgacg ctccccgcac gcattacctc
     1801 atcgggaatc tctctcagtt catccagagc acctggcccg gcactctggt cgagaaatgt
     1861 ggcacttacc gcctcaacga tgaaagcaat accctctgga acgatatcgg aaagccgatt
     1921 gacgagtcca cccactgtct caccctcacc ggcatcccat cgttgaggga cgacaactcg
     1981 tccggccaat cgcaaagcct ggatctcttc atgcagggta tgcagggcca gccgtatacc
     2041 tacatggtcg tggcggattc ggtggcgtgc ggggagctcg acaacatcat cgcccaatgc
     2101 cgtgccctta tcggccaggt ccactccttc aaagaaataa cgctcaatga gacattaacg
     2161 cttgcaagaa cgtggggaag ctcggtcgac gcaagtgaga gctggacgga cggcacctcg
     2221 acggaagact caagcatgaa ggggtggagc gtcggtctcg g
//
LOCUS       DAOQVJ010000276         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59712_length_2257_cov_4.166213, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000276 DAOQVJ010000000
VERSION     DAOQVJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59712_length_2257_cov_4.166213"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKL26_06235"
     CDS             complement(<1..771)
                     /locus_tag="PKL26_06235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNS83327.1"
                     /translation="MRELSRKVAGIAASATIEISNLARQMEKKGIPVISLSIGEPDFD
                     TPQHIKDACIEALKSGQTHYAPSNGIPELLNAISEKINRENGFSCIPEQVLVTCGAKD
                     AIYDAMEAVLNPGDEVIILDPSWVSYEPCVQVAGGRPVHHPLDTKTFQVSESVLDRVT
                     PRTKMVVVNSPSNPSGAVLDDASMKLISDMCTDYDLFALSDEIYEKLVYGKVPRSLAS
                     IGDMAERTITINGFSKAYAMTGWRLGYAVAPLPILRQMA"
     gene            complement(768..1175)
                     /gene="ribH"
                     /locus_tag="PKL26_06240"
     CDS             complement(768..1175)
                     /gene="ribH"
                     /locus_tag="PKL26_06240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNS83328.1"
                     /translation="MTIKLGFVVSEFNRDITYMMEVEGREHAAFLGAEVTECTYVPGA
                     YDMPLAIKRMLKRGKVDAVVTIGCVIEGATQHDEIVVQHAARKIIDLSLEFDKPVSLG
                     ISGPGMTRLEANERIDYARRAVESAVKMVQRSK"
     gene            complement(1234..1692)
                     /gene="ribC"
                     /locus_tag="PKL26_06245"
     CDS             complement(1234..1692)
                     /gene="ribC"
                     /locus_tag="PKL26_06245"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNS83329.1"
                     /translation="MKVGVADTTFARVNMGGIAVDELKKHASVSIERVTVPGIKDLPV
                     ACKKLIEERKCDIVMALGMPGGKEKDRMCAHEASMGLITCQLMTNTHIVEVFVHEDEA
                     KDDRELAWLAGSRTREHAENAIKMVLHPKELIKEAGTGQRQGFPDAGPAR"
     gene            complement(1689..>2257)
                     /locus_tag="PKL26_06250"
     CDS             complement(1689..>2257)
                     /locus_tag="PKL26_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNS83330.1"
                     /translation="LAAPAGLSAVSVSSRAWERMTEPRPFYLDLAAYRKSASAKLMET
                     PYTPAVPLFMALREACLIMEEEGLSARIARHRLMADAVRAAARAWGLPLFPKIDSDHA
                     YSNTATAITLPPGIEDADLRGTVKKMGIEIAGGQDHLKGKIFRIGSMGAVSAPEVLAT
                     LAAVQYALKAQGYAVKDDGVMAAAGVLG"
BASE COUNT          394 a          734 c          645 g          484 t
ORIGIN      
        1 cgccatctgg cggaggatag ggaggggggc gaccgcatag ccgaggcgcc atcccgtcat
       61 tgcataggcc ttcgagaagc cgttgattgt gatcgtccgt tcggccatgt ccccgatcga
      121 cgcaagggac ctgggcacct tcccatagac cagtttctcg tatatctcat ccgagagcgc
      181 gaagagatca tagtccgtgc acatgtccga gatgagcttc atcgaggcgt cgtcgaggac
      241 cgccccggat gggttcgagg gggagttgac caccaccatc ttggtgcgtg gcgtgacccg
      301 gtcaagtacg ctctcactca cctggaatgt ctttgtatcg agcgggtgat gaacgggcct
      361 tccgcctgcc acctggacac acggctcata cgagacccag gaagggtcga ggatgatcac
      421 ctcgtctcct gggttcagga ccgcctccat tgcgtcgtag atggcatcct tggcaccaca
      481 ggtgaccagc acctgctcgg ggatgcagga aaacccgttc tcccggttga tcttctccga
      541 gatggcgttc agcagctccg ggatcccgtt gctcggagca taatgggtct gaccggattt
      601 gagcgcctct atgcaggcat ccttgatgtg ctggggagtg tcaaagtcgg gttccccgat
      661 ggacaggctt atcaccggga tgcccttctt ctccatctgc ctggcaaggt tcgagatctc
      721 gatggtagcc gaggccgcga tccctgctac cttgcgggag agctccctca tttggatcgc
      781 tggaccatct ttactgccga ctcgactgcc cggcgggcat agtcaatccg ctcgttcgcc
      841 tcgagccgcg tcatcccagg tcccgagatg ccaagactta cgggcttgtc gaactcgagc
      901 gagaggtcga tgatcttccg ggccgcatgc tgaaccacga tctcgtcgtg ctgcgtggcc
      961 ccttcgatga cgcacccgat ggtcaccacg gcgtcgacct tcccccgctt cagcatccgc
     1021 ttgatggcca ggggcatgtc gtatgccccg ggcacatacg tgcactcagt gacttcggcg
     1081 ccgagaaatg cggcgtgctc ccgtccttcc acttccatca tgtaggtgat gtcccggttg
     1141 aactccgaga ccacgaaccc aagctttatt gtcatcatgt cctcctcctg aataatctcc
     1201 ttcctgcagg gtcataacga tatcccgaac tgttcagcgg gcagggcctg catcaggaaa
     1261 cccctgcctc tggcccgtcc ctgcctcctt tatgagctct tttggatgaa gcaccatttt
     1321 tatcgcgttt tctgcgtgct cccgcgtgcg cgacccggcg agccaggcca gttcccggtc
     1381 atcctttgcc tcgtcctcgt gaacaaagac ctcgacgatg tgcgtgtttg tcatcagctg
     1441 gcaggtgatg aggcccatcg atgcctcgtg ggcgcacatg cggtctttct ctttgccacc
     1501 cggcatcccg agcgccatca cgatatcaca cttcctctcc tcgatcagct tcttgcaagc
     1561 gacggggagg tccttgatac ccgggacggt gactctctcg atgctgacac tcgcatgctt
     1621 cttcaattca tcaacggcaa tgccccccat gttcacccgt gcaaacgtgg tgtcggcaac
     1681 gccaaccttc atcccagcac cccggcagcc gccatcaccc cgtcgtcctt cacggcatac
     1741 ccctgtgcct tgagggcata ctggaccgcg gcaagagtcg cgagaacctc tggggcgctg
     1801 acggcaccca tgctcccgat ccggaagatc ttccccttca ggtggtcctg cccgccggcg
     1861 atctcgattc ccatcttctt caccgtgccc cgtaagtcgg cgtcctcaat cccgggaggg
     1921 agcgtgatcg cagtggcggt gttggaatac gcgtgatcgg aatcgatctt cgggaacagg
     1981 ggcagccccc acgccctggc tgcggcacgg accgcgtctg ccatgaggcg gtgccgtgca
     2041 atccgggccg aaagcccctc ttcctccatg atcaggcagg cctcccgtag cgccatgaaa
     2101 agggggacgg ctggggtgta gggggtctcc atcagcttcg ccgaggcact cttcctgtac
     2161 gcggcgagat ccaggtagaa tgggcgcggt tcggtcatcc gctcccacgc cctgctgctg
     2221 acagacaccg ctgagagccc ggcgggtgcg gcaaggc
//
LOCUS       DAOQVJ010000277         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59752_length_2256_cov_3.709677, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000277 DAOQVJ010000000
VERSION     DAOQVJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59752_length_2256_cov_3.709677"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            764..1588
                     /locus_tag="PKL26_06255"
     CDS             764..1588
                     /locus_tag="PKL26_06255"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS83331.1"
                     /translation="MADFAANRTEGYAPFAVRFDDLSVGDISGTLWEFGDNTTSFESS
                     PVHRYSQPGTYTVSLEVCGAGGCNLTRKEQFITVREKEPLTAGFLANTTSGAAPLHVQ
                     FQDNSTGNPDSWSWDFGDGATSTEPSPCHLYPLPGVYTVRLVICAEGCMATADAVTVV
                     NVHPQVIGGSVGFFQVMCSAEGAHVFLDGRYMGAIENGTLNIPVYVTAAPARILQVRA
                     EGFLQYYTKIADYPGEGETIVLDVPLQPLSQEEAFFGRFQVPTLMGNLTISGPSLM"
     gene            1661..2092
                     /locus_tag="PKL26_06260"
     CDS             1661..2092
                     /locus_tag="PKL26_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNS83332.1"
                     /translation="MDQCDVAGLSPRKVAYLKYLYERDETARTGEIAAHFTVDPSTVS
                     KTVRELASTGLVDHRPYTGVVLTGKGHEYAEFLVRRHRILGLILTHYGLAGEEACREA
                     ERFESYVSRDAINKMCQSLGHPTRGICGAISHDRGCCVDEG"
BASE COUNT          435 a          661 c          712 g          448 t
ORIGIN      
        1 ggacctgcgg catcgatgtg gaggtgattg gaatgaggga cgaggtggga tcgccttata
       61 tccttccggg aaaagccggc accctcgtgg tgcgggaacc ggtggaagat accgggaacc
      121 aggagagcac tgcccatcca tcctcatcct cccttctccc ccaggcagag ggtcccatga
      181 cacccctgcc gactctggac ccgatctccc ttataaagga aaactcctgt atcccgacgg
      241 actcacctgc cgcatcccct gatggtcatc aggaggcagg gagcgggagc gaagaaccag
      301 atacggatgg cggggtaacg acagggacac tctgcgttga ttccgatcca cggggggcac
      361 agatcttcgt tgacggccag gcctgcggag tgaccccgct ctgcctggca cttgaccggg
      421 gtacccatca cctgtccgtt tcggctgagg ggtattttga cctggagggg gagtatgagg
      481 tgccggcagg acaggtcctc cacctcccgg cctttgtgat ggagcggcct gcgaagtgtt
      541 ggatcatcgc gggggccggg gcccatgggt ccgtgtatcc ttccgggagt gtcgaagtcg
      601 ggagaggtgg atcggccgag ttcgttttcc acgccgatcc cgggtaccgg ctggacgcat
      661 tgcagatcga tggagtttgg tatgatgcca aatctccggt ccggttcgaa ggagtgactg
      721 caaaccacac gttgtggggg gaattctcac cgttcccggc cccatggccg attttgcagc
      781 gaaccggacc gaggggtatg cacccttcgc ggtccggttc gacgacctgt ctgtgggcga
      841 tatatcaggc acgctctggg aattcggtga caatacgact tcctttgaat cttctcccgt
      901 gcatcggtat tcgcagcccg gcacctacac ggtgagcctc gaggtgtgcg gtgcgggagg
      961 gtgcaacctc accaggaagg agcaattcat cactgtcaga gaaaaggaac ccctcacggc
     1021 cggtttcctc gccaatacaa cctccggcgc ggcaccgctg cacgtccagt tccaggacaa
     1081 ctccaccgga aatcccgact cctggtcatg ggactttggc gacggggcga catcaacaga
     1141 accttccccc tgccatcttt acccccttcc cggagtctat actgtccgcc tggtgatatg
     1201 cgccgaggga tgcatggcca cggcagatgc tgtcacagtg gtgaacgtgc acccgcaggt
     1261 gatcgggggg agcgtggggt tcttccaggt catgtgtagt gcagagggag cgcacgtctt
     1321 cctggatgga agatacatgg gcgcaatcga gaacggcacg ctgaacatcc ctgtgtatgt
     1381 cacggctgcc cctgcccgca tcctccaggt gagagcagag ggcttcctgc agtattacac
     1441 caaaattgcg gactatcccg gggagggcga gactatcgtg ctggatgtcc ccctccagcc
     1501 actttcccag gaggaggcgt tcttcgggcg gttccaggtc ccgaccctga tgggaaacct
     1561 cacgatctcc gggccttccc tcatgtgacg aggcgccctg ccgcaggatt gttggtactg
     1621 ctccaacatt ttttatcctc gcacctccca ctgatcccca atggatcagt gtgatgtggc
     1681 gggactctcg ccgaggaagg ttgcatacct gaaatacctg tacgagaggg acgagactgc
     1741 aaggaccggg gagatcgccg cgcattttac cgttgatccc tccacggtct caaagacggt
     1801 ccgggaactt gcgtccacgg gccttgttga ccaccggcct tacacgggag tggtgctcac
     1861 cgggaagggg catgaatatg cagaattcct ggtgaggcgc caccgtatcc ttggcctcat
     1921 cctcacccat tacggcctcg caggagaaga ggcgtgcagg gaggccgagc ggttcgagtc
     1981 atacgtctcc agggatgcca tcaacaagat gtgccagtca ctgggccatc ctacccgcgg
     2041 gatatgcggc gccatcagcc atgacagggg atgctgcgtt gacgaagggt gagggtggaa
     2101 cgcatgggag aactcccggg cctgcccggg atggaaccgt ggtcatgggt gcaggggcag
     2161 cgcgattact atggctctcc ctcctgctgg tcctggccgc gatgtattcg gccgggtgcc
     2221 ttgacaacgg gtcggagaac gggtccgagc tgcagg
//
LOCUS       DAOQVJ010000278         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_59800_length_2255_cov_3.833182, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000278 DAOQVJ010000000
VERSION     DAOQVJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59800_length_2255_cov_3.833182"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..535)
                     /locus_tag="PKL26_06265"
     CDS             complement(224..535)
                     /locus_tag="PKL26_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNS83333.1"
                     /translation="MADMNVSQLFLYEHDAGRIELLVRIVYWIAIGIVAWIYGLITII
                     CLVVQWFHILILGRRSRGLSDFAKGYLEYMVHRMPYMYIMTDRRPAIMPDTVKIYEET
                     G"
     gene            668..741
                     /locus_tag="PKL26_06270"
     tRNA            668..741
                     /locus_tag="PKL26_06270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:702..704,aa:Val,seq:gac)
     gene            951..1691
                     /locus_tag="PKL26_06275"
     CDS             951..1691
                     /locus_tag="PKL26_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS83334.1"
                     /translation="MISLLYIDDEPDLLELGKRFLQREEDFSVDTLDSATLALKRIHE
                     ERYDAIVSDYMMPGMDGISLLKEIRKDGDDVPFIMFTGRGREEVVIEAFTSGATFYIQ
                     KGGDPLVQFAELSHKIRQAVDKRKAEEEVRQVKWEWEHIFNAVENPIVVLSPDHRILA
                     ANRATQEALGRTTPELLGQRCYELFHHRQDPPPTCPLEAMITSKKYEAMQMEVEAMKG
                     RYLVSCTPELDCDGNLIKVIHISTLING"
     gene            complement(1726..1935)
                     /locus_tag="PKL26_06280"
     CDS             complement(1726..1935)
                     /locus_tag="PKL26_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83335.1"
                     /translation="MDIISLAGFLALLIIGIALVLFLIKIVWSLVPAAIVALVVYLFT
                     ASLWWTGAAFLAVVILTFLYKILAK"
BASE COUNT          548 a          588 c          591 g          528 t
ORIGIN      
        1 gatcaggccg ggcgaggagg ccatcatggt cctgaacgtg gacagcgagg tctcctcgga
       61 catgatggat gaaatcaggt cgatgccggg gatattcacc gcagtgtttg ccaagatctc
      121 ggataccctg atctgatacc tcaccctttt tccttcgaat ggacaaagaa aaggaatgcg
      181 gggcgggcat accgtcatcc gcgacctcat ttgttgcggg cttttagccc gtctcctcgt
      241 atatcttcac ggtgtcgggc atgattgccg ggcgccggtc ggtcataatg tacatgtatg
      301 gcatccggtg gaccatgtac tcgaggtaac ccttcgcgaa atccgagagg ccccgcgacc
      361 tcctgccaag aatgaggatg tggaaccact ggacaacgag gcagataatg gtgatgagcc
      421 cgtatatcca ggccacgatc ccgatggcaa tccagtatac aatccggaca aggagttcta
      481 tcctccctgc gtcatgctcg tagagaaaaa gttgtgaaac attcatgtct gccattgtgt
      541 cgcacttcca tcacctcttc cagtcaccct gccatcgctt caatattccc tttccaaacg
      601 cgagaaaaga gaataatttt atactgtgtt cacccacatt tctccgcgca tttcgcagaa
      661 gaattgcggg ctcgtggtct agctggttat gacgtcgcct tgacatggcg gaggtcagga
      721 gttcgaatct cctcgagccc attcgttcct ctcacttctt catgtaagga ttgtcggtat
      781 tctcgcgatt tatccaggac atgatcgccg ctccagcgaa tgtggctccg tttctccatg
      841 tgaagacatc aggtttattc attttttccc ccatgagagt agggatcagg attaaaccat
      901 gcgtgttttt cttctccggg gaggagtaca catggggtgg gttggaattc atgatctcgc
      961 ttttgtatat cgacgatgag ccagatctcc tggaactcgg aaagaggttt ttacagcggg
     1021 aagaggattt ttcagtcgat acccttgatt ccgcgacatt ggcattgaaa cggatccacg
     1081 aggagcgata cgatgcgatt gtctctgatt acatgatgcc cggcatggat ggcatctccc
     1141 tcctcaagga gatccgaaaa gatggggatg atgtgccgtt catcatgttc accgggaggg
     1201 gaagggagga ggtggtgatc gaggcattca cgagcggggc aacgttttac atccagaagg
     1261 gtggagaccc cctcgtccag tttgcagaac tctcgcacaa aatacgccag gcggtggata
     1321 agagaaaggc agaagaggag gtccgccagg tgaaatggga gtgggagcac atcttcaatg
     1381 ccgtggaaaa ccccattgtc gtcctttcac ccgatcaccg gatacttgct gcgaaccggg
     1441 ccacacagga ggcgcttggg cgcacaacgc cagaactgct cggccagagg tgttacgaac
     1501 tcttccatca ccggcaggac cctcctccca cttgcccgct ggaagcgatg ataacctcga
     1561 aaaagtatga agcgatgcag atggaggtcg aggcaatgaa gggccgctac ctcgtctcct
     1621 gcactcctga actcgactgc gatggaaacc tcatcaaggt catccatatc agcaccctga
     1681 tcaacgggta agaaacaccc aaattcgatt gcctgggtgc tctccttatt tggcaaggat
     1741 cttgtaaagg aaggtaagga tcactacggc gaggaatgct gcgcccgtcc accagaggct
     1801 tgcagtaaac aggtacacca ccagcgccac aattgctgcc gggacaagcg accacacgat
     1861 cttaatgaga aagagtacaa gggcaattcc tatgatcaaa agggccagga accctgcaag
     1921 cgatatgata tccattgcaa cctcagggga ttgtgttggg tcttccccta taaggtgttt
     1981 ttgagggcaa aaccggttcc tgcagccctg gtggacatca gaccgggatg accgcattca
     2041 ttccggaccc tggttcccat tccacaggcg aagagaatct ttaaattgaa cgctcaatcc
     2101 atggctatta tggatatttc cgcgataaga aagcgagtat acctgatact tgaacccacg
     2161 tacaaaggtg accggatcag ttactggttt gacgtgttca tcaccgcggt catcctcctc
     2221 tccatctccg tcatggtcct ctcatccgtg agctc
//
LOCUS       DAOQVJ010000279         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_60153_length_2247_cov_3.950274, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000279 DAOQVJ010000000
VERSION     DAOQVJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60153_length_2247_cov_3.950274"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKL26_06285"
     CDS             <1..419
                     /locus_tag="PKL26_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83336.1"
                     /translation="RGDYARCKRSCLEGLDFIPPGSHDIHAEDFLVLIDECKRQSLCD
                     LPVLVYSPLPGDLKDPGSELPEIHLDLDENEKKIVDFLRVHQQATETELRMLLGTRRA
                     GGMVNRIIQKASSAGIVVIEKRGVGKDGEIYAFKKS"
     gene            400..1704
                     /gene="brxD"
                     /locus_tag="PKL26_06290"
     CDS             400..1704
                     /gene="brxD"
                     /locus_tag="PKL26_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HNS83337.1"
                     /translation="MHSRSPDIQPADRLESQNIINALRRGTVPASGLERIAVGLDIEE
                     DVVAAQLDFVARGGGDIKFIRGDYGSGKTFLVARALEIAREKGFVTAHVVISSSSPLH
                     KTKGIYQQICASLRTREEEHAQKSIIDSWLFGIEERILKVSDRSLSDEALEKATLAEI
                     ETALAGISELNTSLAAALRTYYRANNAGDFQNSQAAIGWICAEPNVGREFKQKAGIRG
                     DVDEATAFTFLRGLLRIIVSAGYSGLSVAIDEVETTQSLPRNQREKGYRTLQMIVDSL
                     DRGDLPHSYFIFTGTPAFFDGPRGIRTLPPLYDRISVIQTGEGFRNPRQPQITLAKFD
                     AKKLEQVALKVMDIYSAAYTEVDRSRVSHRFIRAMIKKVTGKFGGRVDVIPRIFLKEY
                     VDVLDKCELYEDFDPWEQYHFETDHLKGELKEEEEAVMVVEF"
     gene            1818..>2247
                     /locus_tag="PKL26_06295"
     CDS             1818..>2247
                     /locus_tag="PKL26_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83338.1"
                     /translation="MSVHLAPFLFVAGCLMLLAGSASGGITLDAQPHTPFIGDRVHIS
                     GISDIPNTIAVYLFVTGPGLNRAGVTLENLHLTAGSGFFTSAFVHPDGRFEYEWNTGF
                     VVGQLIPATYRIYVVNVPLNLDRLTDRDEISLSHIDVTFSR"
BASE COUNT          491 a          641 c          653 g          462 t
ORIGIN      
        1 agagaggaga ctacgctcgt tgcaagcgtt catgccttga ggggctcgac ttcatcccgc
       61 ccggttccca cgacattcac gcggaagatt tcctcgtgct catcgatgag tgcaagcgcc
      121 agtcgctctg cgatctcccg gtcctggtat actcccccct gccaggcgat ctgaaggatc
      181 ccggctccga attgccggaa atccaccttg accttgacga gaatgagaag aagatcgtgg
      241 atttcctgcg ggtccaccag caggccaccg agaccgaact ccggatgctc cttgggactc
      301 gcagggcagg cggaatggtg aatcggatca tccagaaagc atcttctgcg gggatcgtgg
      361 tgatagagaa gagaggggtg ggaaaagatg gagagatcta tgcattcaag aagtcctgat
      421 attcagcctg ccgacaggct ggagagccag aacattatca atgccctgcg aaggggtacc
      481 gttcctgcaa gcggcctgga gaggatcgca gtcggccttg acatagagga ggatgtcgtc
      541 gcggcgcagc ttgatttcgt tgcccgcgga ggaggggaca tcaagttcat acggggcgat
      601 tacgggagcg gcaagacgtt cctcgtggcg agggctcttg agatagcccg tgaaaaaggg
      661 ttcgtaaccg ctcacgtggt catatcctct tcttctcccc tgcacaagac caagggtatc
      721 taccagcaga tatgtgcgag tcttcgcacc cgcgaggagg agcacgcgca gaagtctatc
      781 atcgactcct ggctcttcgg gatagaggag cggatcctca aggtgagcga ccgatcgctc
      841 tctgacgagg ccctcgagaa ggccaccctt gctgagatcg agactgcgct tgccgggata
      901 tcggagttga acacctcgct cgcggcggcg ctccgcacct actaccgggc aaacaacgca
      961 ggggacttcc agaactcgca ggccgcgatc ggctggattt gcgctgaacc gaacgtcgga
     1021 cgtgaattca agcagaaagc cgggatacgc ggggacgtgg acgaagccac ggcgttcacg
     1081 ttcctgcgcg gactgctccg catcatcgtg agtgccgggt acagcgggct ttcggtcgca
     1141 atcgacgaag tggagaccac acagtcgctc ccccggaacc agcgcgagaa aggataccgg
     1201 accctgcaga tgatcgtcga ctccctggac cggggagacc tcccgcattc ctacttcatc
     1261 tttaccggaa ctcccgcgtt cttcgatggt ccccgcggca tccgcacgct cccgcccctc
     1321 tatgaccgca tcagcgtgat ccagacgggt gaagggttca ggaacccccg ccagccgcag
     1381 ataaccctcg cgaagttcga tgcaaagaaa ctcgagcagg tggccctcaa ggtgatggat
     1441 atctactcgg ccgcatacac tgaagtggac agatcgaggg tctctcaccg gttcatcagg
     1501 gccatgatca agaaggtgac gggaaagttc ggcgggaggg tcgacgtgat cccgaggata
     1561 ttcttaaagg agtatgtcga tgtccttgac aagtgcgagc tctacgagga ctttgacccc
     1621 tgggagcagt accactttga gaccgatcac ctcaagggcg agttaaaaga ggaggaggaa
     1681 gcggtgatgg tggtggagtt ctgactctta tcacacgatg taacagcaag acctgaaatg
     1741 gtctggttcc ccggcgaccg ttctccccct gatcccccat cacaatattg atgccttttt
     1801 ttcgactcat attaggtatg agtgtgcacc tcgcaccgtt cctctttgtc gcaggctgcc
     1861 tcatgctgct tgcaggttct gcgtcaggag ggatcaccct cgatgcccag ccacacacac
     1921 cctttatcgg agatcgggtg cacatcagcg gcatttcaga tatccccaac accattgccg
     1981 tatacctctt tgtgacaggc ccaggcctca accgggcggg ggtcaccctg gagaacctcc
     2041 atctcacggc cggatcgggt ttctttacct ctgcatttgt ccatccggat gggagatttg
     2101 aatacgagtg gaatactggt tttgtggttg ggcagctgat ccccgcaacc tacaggatct
     2161 atgtcgtgaa cgtgccgctg aaccttgatc gccttaccga cagggacgag atctcgctat
     2221 cgcacatcga tgtgacgttc tccaggc
//
LOCUS       DAOQVJ010000280         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_60576_length_2237_cov_3.978918, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000280 DAOQVJ010000000
VERSION     DAOQVJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60576_length_2237_cov_3.978918"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..344)
                     /locus_tag="PKL26_06300"
     CDS             complement(18..344)
                     /locus_tag="PKL26_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNS83339.1"
                     /translation="MEELCDALTASRDGTVISLEVSAGSKTERFPAGYNPWRKSIGCL
                     VRAPAVEGKANAAVIRLLASFFSLPQEKVQILSGATSSQKRILLAGMHPDQVQKILES
                     APSRGR"
     gene            complement(506..1804)
                     /locus_tag="PKL26_06305"
     CDS             complement(506..1804)
                     /locus_tag="PKL26_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS83340.1"
                     /translation="MQARCDLVLTNVLQPGGRVADLSLSGGMVCHVGAGYAAQETIDC
                     RGKTVLPGAVDMHVHMRGRDQGYKEDWASGSMSALAGGVTVVVDQPNTIPPLTTPLSF
                     SQRVAEAREASRCHFAINAGYVPGCDLPALWREGAMAFGELFAAASSYAEGLTPGDLG
                     MALEVVAALGGLCTIHAEEVSAGLDSDLHSHHALRTPEGEARAVRRVMDLNTSGCRLH
                     FCHMSCPGSIRNACNSTVEATPHHLFLSLEDFSSLDARGKVNPPLRPVPVRMELLSTW
                     DRIDVLASDHAPHTVPEKAQPFERAPSGIPGVETMVPLALAYCRAKQIPLSSLIEKTS
                     WKPCEILGIKRAGFQVGDRADFAIYPPGDVAIEAEMLHSKAGWTPFEGKRAVFPGTVI
                     MDGEAVFRDGTFFPGHPSWFPGKGFMGTKLPRNSADTAQP"
BASE COUNT          429 a          669 c          673 g          466 t
ORIGIN      
        1 attgcggtgc catgagatca cctcccgcgg ctgggagcac tttccaggat tttctgtacc
       61 tggtcagggt gcatacccgc aaggagtatc cttttctggg aggatgtggc tcccgagagg
      121 atctggacct tctcttgagg cagggaaaag aacgacgcga ggagcctgat gaccgctgca
      181 ttggccttcc cttccacggc aggcgcacgc accaggcagc cgatgctctt cctccatgga
      241 ttgtatccgg caggaaacct ctcggtcttt gaaccggcag atacttcaag ggagatgaca
      301 gtcccatccc ggctcgcggt cagggcatcg catagctctt ccacgtataa gaaaggaggt
      361 gtcgcccggc attaacaact gcggatcact ggtgggctga ccccgtccct cccctgttta
      421 tacccattgc cgggcggtgc ctgtcgcaca accgggttgt cccgtagttc atatccaggc
      481 gtccgccaat cgatcacccg gggacctatg gctgtgctgt gtcggcacta ttcctgggga
      541 gttttgttcc cataaagcct ttccccggga accatgaggg gtggcccggg aaaaaggttc
      601 catcccggaa gaccgcctcc ccgtccatga tcacggttcc tggaaagacc gccctcttcc
      661 cctcgaacgg ggtccagcct gccttgctgt ggagcatctc tgcctcaatc gccacgtcgc
      721 cgggagggta gatcgcaaag tccgccctgt cgcccacctg gaaaccagct cttttgattc
      781 ccaggatctc gcacggtttc caggaggtct tctcaatcag cgaggagagg gggatctgct
      841 ttgcccggca gtaggccagt gccaggggca ccatggtctc cacgcccggg attcccgaag
      901 gcgcgcgttc gaaaggctgc gctttctctg gcacggtgtg gggggcatga tccgatgcca
      961 gcacgtctat cctgtcccag gtggagagga gttccatccg gaccggaacc gggcggaggg
     1021 gggggttcac cttccccctt gcatcgagcg atgagaagtc ctcgagcgag aggaacaggt
     1081 ggtggggtgt tgcctccacg gtggaattgc aggcgttccg gatcgatcca gggcagctca
     1141 tatggcagaa gtggagcctg catccgctcg tgttcaggtc catgacacgc cggactgccc
     1201 tggcctcccc ctccggagtg cggagcgcgt gatgagagtg gaggtcgctg tccagcccgg
     1261 ccgaaacctc ctcggcgtga atggtacaga gtccccccag tgcggcaacg acttcgagcg
     1321 ccatcccaag gtcgccgggt gttagtccct cggcataact cgaagcggcg gcaaagagct
     1381 ccccaaaggc catcgccccc tctctccaca gggctgggag atcgcatccc gggacatacc
     1441 ccgcattgat ggcgaagtgg cacctggagg cttcccgtgc ttcggccacc ctctgcgaaa
     1501 acgacagggg ggtggtaagg gggggaattg tattcggctg gtccacgacg accgtgactc
     1561 ctcctgcgag cgcactcatg ctcccgcttg cccagtcctc cttgtatccc tggtccctgc
     1621 ctctcatgtg gacatgcatg tcgaccgcac ctgggaggac cgtcttcccg cggcaatcga
     1681 tcgtctcttg ggctgcatac ccggctccca cgtggcagac cattcctccc gagagcgaga
     1741 ggtctgccac tcttccaccg ggttggagga catttgtgag gacaagatcg cagcgggctt
     1801 gcatgggacg tctttcacct gggtgttggt gccggaacga gatgatactt ggcccccatt
     1861 tctcctcttc gtcaagttca cgaacaggga agaatgccct ttcttcttat ttggggaggc
     1921 gttccgggga gaggcactcc ggactgcttc agacagtctt cctatcggga agaaggtatt
     1981 tgccccgtaa ttttcccatt tctcccaggg gagagaagaa ttaatcagga gaggcttcga
     2041 agactcatca cagaagatta gagaggaaaa taagatgaga tcgatcgcaa tcattctctg
     2101 cgtactctgc tttatcgcgg taggcatcgg gtgcgtcggt ggagaagaca ctcccgtcga
     2161 caacgtgaac attgaaacaa cctccacccc ggcagagggg atggcgtacc gcgagcagat
     2221 cggcggagat gtggggt
//
LOCUS       DAOQVJ010000281         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_60943_length_2228_cov_4.399908, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000281 DAOQVJ010000000
VERSION     DAOQVJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60943_length_2228_cov_4.399908"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..731)
                     /locus_tag="PKL26_06310"
     CDS             complement(69..731)
                     /locus_tag="PKL26_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS83341.1"
                     /translation="MPTCAACGADMKEGATFCPSCGMKQGQDARREDALFWTAKARVI
                     SSRPVVTQLIMVFGVGFLFVMVLLLILDVESAIMLAPYVIATAVFIIVLGLIIAAAIQ
                     FFTKGGLTTEFAVTPKGIGYRAGKESKAINRATLAGTAIGGSLAGAGGSMINISREMD
                     FMSWAEVKSVTIYPRERSLVFYRKALIFPFALYCTPENFEQVKTLVKKYASKARFREK
                     RW"
     gene            939..1439
                     /locus_tag="PKL26_06315"
     CDS             939..1439
                     /locus_tag="PKL26_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS83342.1"
                     /translation="MPQVHIIRMPFCTSCGQQLADTDRFCTSCGAPMEPADKPAPSPA
                     DTKEIRWTNSVLGMTSDFFVNLEGAGQEASKKTSTVNTGIAVAGGLLGSLSAAGGGMI
                     VKSRENDFIRWNETRSVALNRKKKTVTVTRKSLIFPIRLYCTEENYEQVAAFIRQHVN
                     PVLIKE"
     gene            1648..>2228
                     /locus_tag="PKL26_06320"
     CDS             1648..>2228
                     /locus_tag="PKL26_06320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNS83343.1"
                     /translation="MGMSGLLVLAIAPAIFILCYFYLRDRYEPEPLGWVVKVFLVGAI
                     MVIPAALIETPFPSGLLTAVFVAPIVEESLKFAAVYFTVYRDEEFDEPVDGIVYASAA
                     ALGFAMLENVMYVMEGGVAVGIVRAVVSVPAHALFSSIWGYSLGIARFRHEKDRLFLI
                     AIGLAGAMAFHGIFNLMASDSELLGLIVILVLLV"
BASE COUNT          463 a          630 c          619 g          516 t
ORIGIN      
        1 caattgagaa atgatttggt gctattttgg aaacgatatc gcaagggggc taatgccatt
       61 atatccgttc accaccgttt ttccctgaaa cgcgctttag aagcgtattt tttcacgagt
      121 gttttgacct gctcgaaatt ttcgggggtg cagtagaggg cgaacgggaa aataagcgct
      181 ttcctgtaga agacgaggga tcgctcccgg ggatatatgg tgacgctctt cacttcggcc
      241 cagctcatga agtccatctc gcgactgata ttgatcatgc tccccccggc tcctgctagc
      301 gaacctccga tcgcggtccc tgcaagagtt gcgcggttga tagcctttga ctccttcccg
      361 gcccggtagc cgataccttt cggggtgacg gcgaattccg tcgtgaggcc gcccttcgtg
      421 aagaactgga tggcagcagc gatgatcagg ccgagaacga tgatgaatac tgccgtcgcg
      481 atgacataag gcgcgagcat tatcgcagac tccacatcga ggatgaggag gagaaccatg
      541 acgaagagga acccgacccc gaagaccatg atcagctggg ttaccactgg ccggctggag
      601 atgacccggg cttttgctgt ccagaacagg gcgtcctccc gacgtgcgtc ctgtccctgt
      661 ttcatcccgc atgatgggca gaaggttgcc ccttccttca tgtctgctcc gcacgctgca
      721 caggttggca ttgcatcgct cctttatcca gtatgtgttt tccataaggt aaaaaaaggg
      781 cacatatatc cagggaaagg aggttccctc cgtaagatct tgtacctgtc cctcatccat
      841 tgcgaggttc caactgcggg aagggcagga aacgagcagg ctggctctac tccaagcgtc
      901 cccccgggat gtaacctttg catgccaaat catttttcat gccacaggtc cacatcatcc
      961 gcatgccgtt ctgcacctcc tgcggacagc agcttgctga caccgaccgc ttctgcacct
     1021 cctgcggggc tcccatggag ccagcggata agcctgcccc atcacccgcc gatacgaaag
     1081 agatccggtg gaccaacagt gtcctcggca tgacctcaga cttcttcgtg aatcttgaag
     1141 gggcggggca ggaagcatcg aaaaagacca gtactgtcaa tacaggaata gccgttgcag
     1201 gcggcctcct tgggagcctg tccgcggcag ggggcgggat gattgtcaag agcagggaga
     1261 acgatttcat caggtggaat gagacacgga gcgttgccct caaccggaag aagaaaacgg
     1321 tgacggtaac ccgaaaatcc ctaatcttcc ctatcaggct ctattgcaca gaggaaaact
     1381 acgagcaggt cgccgcgttc atcaggcagc acgtgaaccc tgttctcatc aaggaataac
     1441 agggtgagga gaccaatccc cccaacacgc gatgtgcact gcagtccagc catatcagga
     1501 tgcgaggggt aatgctactc tcggtttttt tattcacctg ccgttccggg gatacctgaa
     1561 aacagcgcat tcgtgcagtt gcggtccagg gatcatatca ggccgttccg tttcgcagcg
     1621 gttatcacgt gccggaaaat accggtaatg ggaatgtccg gcctccttgt ccttgccatt
     1681 gcgccggcga tatttatcct ctgctacttc tacttgcggg accgctacga gccagaacct
     1741 ctcggctggg tagtgaaggt cttcctggtc ggcgccatca tggtgatacc agccgctctg
     1801 atcgagaccc cgttcccctc cggcctcctc accgcggtct tcgtcgctcc cattgttgag
     1861 gagtcactca agttcgcagc ggtctatttc accgtgtatc gtgacgagga atttgatgag
     1921 ccggtggacg ggattgtcta cgcctcggct gcagccctcg ggtttgctat gcttgagaac
     1981 gtgatgtacg taatggaggg cggggttgcc gttggaatcg tccgggccgt ggtctcggtc
     2041 cctgcccatg cccttttttc ctcaatctgg ggctacagcc tcggtatcgc ccggttcagg
     2101 catgagaagg accggctgtt cctgattgcc attggccttg ccggggcgat ggccttccac
     2161 ggcattttca acctcatggc atcggattcc gagctgcttg gcctgatcgt gatcctcgtc
     2221 ctgctggt
//
LOCUS       DAOQVJ010000282         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_61092_length_2225_cov_4.120737, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000282 DAOQVJ010000000
VERSION     DAOQVJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61092_length_2225_cov_4.120737"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..384
                     /locus_tag="PKL26_06325"
     CDS             151..384
                     /locus_tag="PKL26_06325"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNS83344.1"
                     /translation="MPGFDGTGPSGRGPMTGWGRGRCMAYGVPAAAQGQAQVAAPFPP
                     GYAPPAYYGLGRGGIPRGCGRGFGGGRRRRCFW"
     gene            435..668
                     /locus_tag="PKL26_06330"
     CDS             435..668
                     /locus_tag="PKL26_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83345.1"
                     /translation="MTEHTKDNQGVSLGLPSSSQTPRTHGTKSQVTSIPLRTALRIAR
                     EQQRSKSSRRKIFREEPERYLDIVHLMKDPGSE"
     gene            774..1151
                     /locus_tag="PKL26_06335"
     CDS             774..1151
                     /locus_tag="PKL26_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNS83346.1"
                     /translation="MDEYIPAACAPRRRGRPRTQRVIGNMFSSRCYSPQCWPHEERAV
                     VTILPEEVELLRLVDLEGMEQEEAAAALGVSRRTAWRDLHEARRKVADALTTGKSLQI
                     GGCSRASRGICPRKCPRFPGNSP"
     gene            1385..>2225
                     /locus_tag="PKL26_06340"
     CDS             1385..>2225
                     /locus_tag="PKL26_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS83347.1"
                     /translation="MKHPFISVQNLSMSFSGKKVLDNLSFDIPEGEIIGIIGRSGAGK
                     SVLMHLLRGVDEPPTEGSIVYHLSRCPKCSYIGLRRELSKPCPRCGEQLFAIDVDFWE
                     PENEQLKSLVMRRTAIMFQRTFALYGDDRVIENVLRALDDVGYSQDGAVNRAADLLDQ
                     VRLSHRMMHIARDLSGGEKQRVVLARQLAKEPCLLFADEPTGTLDPDTARLVHQMLRS
                     AAETTNMGIVVTSHFSRVIQDIATRAMLLEEGRIEKIGAPAEVINHFLRDFHEEENIS
                     EVEL"
BASE COUNT          480 a          665 c          657 g          423 t
ORIGIN      
        1 ctgcagtgca gtcctcgcca cgcacggcta ttcataagag agcaacgacg actgatcccc
       61 ctttccgccg ggttgccctc aacaacacat tcatgtattt ttactcatat gagtaattaa
      121 gggcccaggg cccacaggca ggttatgaaa atgccaggat ttgatggaac aggaccctcc
      181 ggacgcggtc ctatgacagg atggggaaga ggccgatgca tggcatacgg ggtccccgcg
      241 gccgcacagg gccaggcaca ggttgctgca ccgtttccac cgggttacgc accccccgca
      301 tattatggtt tgggcagggg tggcatcccc cggggttgtg gaagagggtt tggcggcgga
      361 aggcgaaggc gctgcttctg gtgatgaggc atcaccggat atccacaagt gagcaatcct
      421 gcagggtcgg cgctatgact gaacacacga aggacaacca gggagtctcc cttggactcc
      481 cttcttcatc tcaaacacct cggacgcacg ggacaaaatc ccaggtcacc tccatcccgc
      541 tgcggaccgc gcttcgcatc gcgcgcgagc agcagagatc gaagtcctcg cgaaggaaga
      601 tattccgtga agagcctgag cggtacctgg acatcgtgca cctgatgaaa gacccgggct
      661 ccgaataggt atttcatgcc gcgggagtcc cccaatcttt ctcttatctt ttcttgaccg
      721 cagtcccgca aaggcagaag gctagatgtg ccccggggga gacaggtaca ggcatggacg
      781 aatacattcc cgctgcatgc gcacccagga ggagaggaag gcccaggacg cagcgcgtga
      841 tcggaaacat gttctcttcc cgttgttatt ccccccagtg ctggccccac gaggagcggg
      901 cagtagtcac gatccttccc gaggaggtgg agctccttcg ccttgttgac ctcgagggga
      961 tggagcagga ggaggctgca gcggccctcg gtgtctcgcg aaggaccgcg tggcgggacc
     1021 ttcatgaggc caggagaaaa gtggcggatg cactcacgac aggaaagagc ctccagatcg
     1081 gcggctgctc ccgggcatcc agggggatat gtccgaggaa atgcccccgg ttccctggca
     1141 actctccttg agagggtttc ctcgccgacc gtccccgcca cccccgttat gcacgtgtct
     1201 ccacatccct gaccggatcc tcccctgcag agggccggag gtgacaatta catcatcttt
     1261 atttctttct actcatatga gaaataatag atgacgccac gcggagagac cgggggtcaa
     1321 gtgatctctg ctgcctcccg gggtcagctc ctgcagttgg aaaccatgca agtgtgagga
     1381 tgatatgaaa caccccttta tttcagtcca gaacctctca atgtcatttt caggaaaaaa
     1441 agtccttgat aacctttcgt tcgatattcc tgaaggtgag ataatcggga tcatcgggag
     1501 gagcggtgcc ggaaagagcg tgctgatgca cctgctgcgc ggtgtggatg agccccccac
     1561 ggaggggtcg atcgtctacc acctctccag gtgtccgaaa tgctcctaca tcggcctccg
     1621 gcgcgaactc tcaaaaccct gcccgcgctg cggtgagcag ctctttgcaa tcgacgtgga
     1681 cttctgggag cctgagaacg agcaattaaa gagcctcgtc atgcggcgca cggccatcat
     1741 gttccagcgg acattcgcgc tctatggtga cgacagggtc atcgagaacg tgctccgggc
     1801 actcgatgat gtcgggtact cccaggacgg tgcggtcaac cgcgcggccg acctcctcga
     1861 ccaggtacgc ctctcccacc gcatgatgca catcgcacga gacctctcag ggggcgagaa
     1921 gcagcgggtg gtgctggcaa gacaactcgc aaaggagccc tgcctcctct ttgccgacga
     1981 gccgaccggg acgcttgatc ctgacaccgc ccggctcgtg caccagatgc tccggtctgc
     2041 tgcagagacc acgaacatgg ggatcgtcgt cacctcccac ttctcccggg tcatccagga
     2101 catcgccacc agggccatgc tcctcgagga ggggaggatc gagaagatcg gggcccccgc
     2161 agaggtgata aaccacttcc tgcgggactt ccacgaggag gagaacatat cggaggtcga
     2221 actgg
//
LOCUS       DAOQVJ010000283         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_61183_length_2223_cov_3.916052, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000283 DAOQVJ010000000
VERSION     DAOQVJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61183_length_2223_cov_3.916052"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1138
                     /locus_tag="PKL26_06345"
     CDS             62..1138
                     /locus_tag="PKL26_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNS83348.1"
                     /translation="MKGAVLVCGFATRHVARSAHAAGYTVYAVDHFCDQDLSWYTADR
                     ERFENLSDLPGAVENICSRHRVDWLVPTSGAETLPGQIPVLGTPPGRAARFMDKLLTQ
                     EFFESLQVPVPKILGPGCYPAMVKPRAGSGGWRNMVVHSDAELACWLETFEYPPYLSQ
                     EVVTGTPASVCCVADGKGNARAIAANYQILREKAGAQFGFSGSVTPLVHPQAQQMAEM
                     AEKIASASGCTGTFGIDFVIGDEPWAIEVNPRFQATLDTVEAATGLNLFSAHARACQG
                     EIPSSRPSPVGFAVRRILFSDRDMMVKNDLSFLAPAVADIPWPGSFFEEGHAVISVYG
                     WGRSGEAAMELLDTHMKRVRQYMG"
     gene            1146..1658
                     /locus_tag="PKL26_06350"
     CDS             1146..1658
                     /locus_tag="PKL26_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HNS83349.1"
                     /translation="MEEILANTPIRAYLARLIGEEGISLLESFPREGEHSDEELAAQT
                     GINLNSVRHTLYTLYEKRLAEYRRIKNNETGWLTYLWHLRIDRVYDVIAEDMEAILEK
                     LEAREKFEEENDFYICKSCGFMFTFSQAMDCQFQCPSCETGLEHFDNEILLRALKRRI
                     RSMKESLGHA"
     gene            1651..2157
                     /locus_tag="PKL26_06355"
     CDS             1651..2157
                     /locus_tag="PKL26_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNS83350.1"
                     /translation="MPDSEGHWEALLRSSGCSKRVIAHCRSVRDLAIEYLHDDIMDRE
                     VLEAGALLHDIGRGVTHGLPHAQAGADLCRRRGVPEEVARVVERHIGAGLTADECTLL
                     GLLPIDCMPRTLEERVVAHADNRVAGTRRICLDERLLHAIHLKKRQKQRLYRLWQEME
                     MFRQTPGT"
BASE COUNT          466 a          646 c          690 g          421 t
ORIGIN      
        1 agtttcccgg ggcaaagata cgggtggtcc cttcacccga agggaagatc accgaggagc
       61 aatgaagggc gcagtgctcg tctgcggatt tgcgacccgg cacgtggcca ggtccgccca
      121 tgccgccgga tacactgtct acgcagtgga ccacttctgc gaccaggacc tctcctggta
      181 tacagccgac cgagagcggt tcgaaaacct gtcagacctt cccggcgccg ttgagaatat
      241 ctgcagccgg catcgcgtgg actggctggt tcccacttcg ggcgcagaga cgctccccgg
      301 gcagatcccc gtgctcggga cgcctcccgg gcgggcagcc cggttcatgg acaagctgct
      361 cacccaggag ttcttcgagt cccttcaggt ccctgtccca aaaatactcg ggccgggctg
      421 ctaccctgcc atggtcaagc cgagagcagg gtctggcggc tggcgcaaca tggtggtcca
      481 cagcgatgcg gaacttgcat gctggcttga gaccttcgag tacccgccct atctttccca
      541 ggaggttgta acaggtaccc ctgcatcagt ctgctgcgtg gcggacggga agggcaacgc
      601 gcgggcaatt gccgcaaact accagatcct cagggaaaaa gccggagcac aattcgggtt
      661 ctccggctcg gtcactcctc tcgttcaccc gcaggcacag caaatggccg agatggcaga
      721 gaagattgca tcggcctctg ggtgtacagg cactttcggc atcgactttg tcataggaga
      781 tgagccctgg gcaattgagg tgaatccccg gttccaggct acgcttgata ctgtcgaggc
      841 cgcaacaggg ctgaacctct tctcggccca tgcgcgtgcc tgccaggggg agatcccttc
      901 cagccggcct tcgccagtcg ggtttgcggt acgccgcatc ctcttttcgg acagggacat
      961 gatggtgaag aatgatctct ctttcctcgc acctgcagtg gcagacatac cctggcccgg
     1021 cagtttcttc gaagagggcc atgcggtgat cagcgtgtat gggtggggca ggagtggaga
     1081 agcagccatg gaactgctgg atacacatat gaagagggtc cgacaatata tgggttgatt
     1141 gccctatgga agagatcctc gcaaataccc cgatacgtgc atacctcgcc cggctgatag
     1201 gagaggaggg catctctctc ctggagagct tcccgaggga aggcgagcat tccgatgagg
     1261 aactggccgc gcagaccggg atcaacctca actcggtcag gcacaccctc tatacccttt
     1321 acgagaagcg gctcgccgag taccgcagga tcaagaataa cgaaacggga tggctcactt
     1381 acctctggca cctccgtatt gacagggttt atgacgtgat cgccgaggac atggaagcga
     1441 tacttgagaa gctggaggcc agggagaagt tcgaggagga gaacgatttc tacatctgca
     1501 agtcgtgcgg gttcatgttc accttcagcc aggcgatgga ctgccagttc cagtgcccct
     1561 catgcgaaac gggactggaa cactttgaca acgagatcct cctccgtgca ttgaagaggc
     1621 ggatcaggag catgaaagag tcgctcggtc atgcctgatt cggaggggca ctgggaggct
     1681 ctcctgagat cgtccggctg cagtaagagg gtgatcgcac actgcagatc tgtccgtgac
     1741 ctggcaatcg agtacctgca cgatgatatc atggaccggg aagtcctgga agcgggcgcc
     1801 ctcctccatg acatcgggag aggtgtcacc catggcctgc cccatgcgca ggccggagca
     1861 gatctctgcc gcaggcgcgg agtgccggaa gaggttgcga gggtggttga gcgtcacatt
     1921 ggtgcgggtc tcaccgcgga cgagtgcacg cttctcgggc tccttcccat cgactgcatg
     1981 ccgcgcaccc tggaggagcg ggtagtcgca catgcggaca acagggttgc cggaacccgc
     2041 cgcatctgcc tcgatgagag gctgctgcac gcaatccacc tgaagaagag gcagaaacaa
     2101 cgcctctacc gtctctggca ggagatggag atgttcaggc agacccctgg aacgtgatct
     2161 ggcgtacctg ggggagacgc ctgatccctt cgatcaccgc cgggggaaag tccccctcga
     2221 taa
//
LOCUS       DAOQVJ010000284         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_61317_length_2220_cov_3.911316, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000284 DAOQVJ010000000
VERSION     DAOQVJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61317_length_2220_cov_3.911316"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /gene="carB"
                     /locus_tag="PKL26_06360"
     CDS             complement(<1..1319)
                     /gene="carB"
                     /locus_tag="PKL26_06360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNS83351.1"
                     /translation="MPKKAHIKKVLLIGSGPIQIGQAAEFDFSGSQACRALREEGVKV
                     VLVNSNPATIQTDPEMADEIYIEPIRAEIIEKIIDKERPDGILSGMGGQTGLNMTAEL
                     AERGALKGVEILGTPLEAIYQGEDREKFRALMEHIGEPVPRSMILNRLDQVEEALRTV
                     GLPAIIRPAYTLGGSGGGIAHTREELVRIIEIGLSRSRIHQVLVEESVVGWKEIEFEV
                     MRDAGDTCIIVCGMENVDPMGIHTGESVVVAPILTLRDDEFQMLRTAAIKIIRALDVQ
                     GGCNIQFAYQDGEYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLRLDEITNSV
                     TGCTPASFEPAIDYIVVKVPRWPFDKFKNADRTLTTAMKSTGEVMAIGRTCEEAFKKA
                     LRSIDTDTDTHTNPNEIRMILSRPTDERFTTLFDAVRAGFSVEEIADL"
     gene            complement(1319..2137)
                     /locus_tag="PKL26_06365"
     CDS             complement(1319..2137)
                     /locus_tag="PKL26_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286080.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNS83352.1"
                     /translation="MALSTGDTLNNGHYRIVRPLGRGGFGFVYLAEDTLLGERVAIKE
                     LIPALVGDEDAGRAVDMARRAKPISDAELIPAVSCTEPYHIAGRGKRIAVIDLGIKKN
                     MIISLGHRDADLHVFPYNSAPDQVMACRPDALFISNGPGDPIHATDAIRCVKDLAGTL
                     PIYGICMGNQICGLGLGGQTYKMKFGHRGTNQPVRYKDGSIYITTQNHGFAVAADTLP
                     EGANVLYSNVNDGTLEGFEDPYLEITCVQFHPEAHGGPRDTEIPFFDTMFRRLG"
BASE COUNT          375 a          694 c          680 g          471 t
ORIGIN      
        1 agatccgcga tctcctctac ggagaacccg gcgcggacag catcaaagag cgtggtgaac
       61 ctctcgtcgg tggggcggga gaggatcatg cggatctcgt tcggattggt atgcgtgtcg
      121 gtgtccgtat cgatggaacg cagcgccttc ttgaacgctt cctcgcacgt ccgccctatg
      181 gccatcacct cacccgtgct cttcatcgca gtcgtcaggg tccggtcggc attcttgaac
      241 ttgtcgaacg gccaccgggg gaccttcacg acaatgtagt cgattgcagg ctcgaacgag
      301 gcaggggtgc agcccgtgac gctgttggtg atctcgtcca ggcgcaggcc aatcgcgatc
      361 tttgcagcca cccgggcaat cgggtatccc gtggccttcg aagcgagtgc ggatgaccgc
      421 gagacgcgtg ggttcacctc gatgacccgg tactcgccat cctggtatgc aaactggatg
      481 ttgcagcccc cctgcacgtc gagcgccctg atgatcttga tggccgccgt ccggagcatc
      541 tggaactcgt cgtcccgaag cgtgaggatc ggggctacca ccacgctctc gccggtgtgg
      601 atgcccatcg ggtcaacatt ctccatgcca cagacgataa tgcaggtgtc tcccgcgtcc
      661 cgcatcacct cgaactcgat ctccttccag ccaaccacgg actcctccac cagcacctgg
      721 tggatgcggg acctggagag cccgatctcg atgattcgga ccagttcttc ccgggtatgg
      781 gcaatacccc cacccgatcc gccgagagta tacgccggcc ggatgatggc agggaggccg
      841 accgtcctga gcgcctcctc tacctggtcc aggcggttga ggatcatgct cctcgggact
      901 ggctctccta tatgctccat cagggcacgg aacttctcgc ggtcctctcc ctggtagatg
      961 gcctcgagag gagtcccgag gatctccact cctttcagcg ccccccgctc tgcaagctcg
     1021 gcggtcatgt tcaggccggt ctgccccccc atccccgaga ggatcccgtc gggcctctcc
     1081 ttatcgatga tcttctcgat gatctctgcg cgtatgggct cgatgtagat ctcgtctgcc
     1141 atctcggggt ctgtctggat ggtggcgggg ttcgagttca ccagcaccac cttgacgccc
     1201 tcctcgcgaa gcgccctgca tgcctgcgag cccgagaagt cgaactctgc ggcctgcccg
     1261 atctggattg gcccggagcc gatcaggagg acctttttta tatgcgcctt ctttggcatc
     1321 agcccagcct cctgaacatg gtgtcaaaga aaggaatctc cgtgtcccgt gggccgccat
     1381 gggcctcggg atggaactgg acacaggtga tctcgaggta cggatcctcg aatccctcca
     1441 gggtgccgtc gttcacgttt gagtagagta cattcgcccc ctccgggagt gtgtctgcgg
     1501 cgacggcaaa gccgtggttc tgggtcgtga tgtaaatgct cccgtccttg tagcggacag
     1561 gctggttggt gccgcggtga ccgaacttca tcttgtaggt ctggcctccg agcccgaggc
     1621 cgcagatctg gttccccatg caaatcccat aaatcggaag ggtccccgcg aggtctttta
     1681 cgcaccggat ggcatcggtg gcatggatgg ggtctccggg gccgttgctg atgaagagag
     1741 cgtcggggcg gcaggccatg acctggtcgg gcgcgctgtt gtaggggaag acatggaggt
     1801 ccgcatcgcg gtgccccagg ctgataatca tgttcttttt tatcccaagg tcgatgaccg
     1861 cgatcctctt tcctcttccg gcaatgtggt acggctctgt gcatgagacg gcgggaatta
     1921 gttctgcgtc cgatatcggc tttgctctcc tggccatgtc cactgcgcgg cctgcgtctt
     1981 catcgccgac cagggcgggg atcagctcct tgatggcgac gcgctccccc agcagcgtgt
     2041 cctcagccag gtagacaaag ccaaagccgc cgcggcccag cgggcggacg atgcggtagt
     2101 ggccattatt gagcgtatca cccgtactca gggccattga gctctcgaaa gtgcgcggga
     2161 cgtaacatct cgcgtgttga tcgggccaag cggcggacgg cagggcacgc ccggtggctg
//
LOCUS       DAOQVJ010000285         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_61524_length_2215_cov_4.651389, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000285 DAOQVJ010000000
VERSION     DAOQVJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61524_length_2215_cov_4.651389"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..389)
                     /gene="eif1A"
                     /locus_tag="PKL26_06370"
     CDS             complement(87..389)
                     /gene="eif1A"
                     /locus_tag="PKL26_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HNS83353.1"
                     /translation="MVDETAPPGMETIRVRLPKKRNREQFAIAELMLGSNHIKVRCID
                     GVTRMGRIKGKIKKRAWIREGDTLIITPWSFQDEKCDISYRYTKPQVDWLRRNRYL"
     gene            607..1389
                     /locus_tag="PKL26_06375"
     CDS             607..1389
                     /locus_tag="PKL26_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83354.1"
                     /translation="MQSRVFFSLTLLFLVGCLAFSGCLTSPPVEEPVQETPTTVPTTN
                     PATAGPLTETATPTPTPTAIGLSSEDINLHFMDVAFGGGNLFLERLPLSSKKTTISLN
                     AGTEPDKIVVESFATRFNVLSQSNKLFENIKEGQTGDIRIKFLPPEGLKAIDLTSEMG
                     WLNEEYTCNGVTCAKIKGYDIYLNAEMEGDQRVHYLLRSLLYTLGFKGASLRYPDSIF
                     AYPDNAATELSFLDEKALQVMYGLGMDNGMTVDDVKRVLFFR"
     gene            1471..2091
                     /locus_tag="PKL26_06380"
     CDS             1471..2091
                     /locus_tag="PKL26_06380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS83355.1"
                     /translation="MDDIYRKIPPGKIPWNIQSPPDILVDLVESRTITPCNTIDLGCG
                     AGNHAVYLAGRGFRVTGVDMSPAAIEMARKNAEKRGVLCTFIVADVLDDLGGVGGPFQ
                     FAYDWELLHHIFPPDRDRYVRNVSNLLVSGGMYLSVSFHEDDPHFGGSGKYRRTPIGT
                     TLYFSSGEELFDLFSPHFTIIESKVRTIPGTSGTHEANCFLMVKVS"
BASE COUNT          465 a          648 c          571 g          531 t
ORIGIN      
        1 ctttataaga agatcgagac gtggcaggta ctccggcagt acctccggga ctgagtggta
       61 actgcacccc ggggaaaaag gaggccctag aggtaccggt ttctccgcag ccagtcgacc
      121 tgcggcttcg tgtaccgata ggagatatcg cacttttcgt cctggaagct ccacggggtg
      181 atgatgagcg tgtccccttc ccggatccag gcccgcttct tgatcttgcc cttgatccga
      241 cccatgcggg tgaccccgtc aatacaccgg accttgatat ggtttgagcc gagcatcagt
      301 tcggcgatag caaactgttc cctgtttcgt ttttttggca gtcgcacgcg gatggtctcc
      361 attcccggcg gtgccgtttc atcaaccact acttcttcgt tcctgtcttc tcttttataa
      421 ttactaatag gccagcaact ccactgggaa catttttttt atccccgtgg gtttagtatt
      481 ggcgttcctg ccttatacca gtgatcatgc gggtcggctc ctgtatcaca agggtgttga
      541 tgcagggtga agggcgtttt ttcccgcaat gccaaccatt atgcatcctt gtaatgaaat
      601 tcattcatgc aatcacgggt atttttttcc ctgacattac tcttcctggt gggctgcctg
      661 gcgttctccg gttgccttac ttcccccccc gttgaggagc ccgtgcagga aaccccgacc
      721 accgtgccga ccaccaatcc ggccactgcc ggccccctca cagagaccgc aacacccact
      781 cccaccccca ctgcgatagg gctctcgagc gaggacatca acctgcactt catggacgtg
      841 gcgttcggag gagggaacct tttcctggaa cggcttcccc tctcctcgaa gaagaccacc
      901 atctccctca atgcgggaac cgaacctgat aaaattgttg tcgaatcgtt cgcgacgagg
      961 ttcaatgtcc tctcccagtc aaacaagctc ttcgagaata tcaaagaggg acagaccggt
     1021 gacatccgca tcaaattcct ccctcccgag ggattgaagg cgatcgatct cacatccgaa
     1081 atggggtggc tgaacgagga atatacctgc aatggtgtta cctgtgccaa gatcaagggg
     1141 tatgacatct acctgaatgc agagatggaa ggcgaccagc gtgtccacta cctgctcaga
     1201 tcgctcctct atacgctcgg gttcaagggg gcatccctcc ggtacccgga cagcattttc
     1261 gcctacccgg acaatgcggc gaccgagctc tccttcctgg atgagaaggc cctccaggtg
     1321 atgtacggcc tgggcatgga caacgggatg acggtcgatg atgtcaagag ggttcttttc
     1381 ttcaggtgac cgctcctccc attctttttg gcatatgaac ccccgtcttc tcgaggatga
     1441 ggcgtctgcc tgacggacat ggctcctgac atggacgata tctaccggaa gattcctccc
     1501 gggaagatcc cgtggaacat ccagtcgccc cctgatatcc tggtcgacct ggtcgaatcc
     1561 cgcacgatta ccccctgcaa cacgattgac ctgggctgtg gggcgggcaa tcatgcggtg
     1621 tacctggcag gcaggggatt tcgggtcact ggggttgaca tgtcgcctgc ggcaatcgag
     1681 atggccagga agaacgccga gaagcgggga gtattgtgta cttttattgt cgcagatgtt
     1741 ctcgacgacc tgggaggtgt cgggggcccg ttccagtttg cctacgactg ggagctcctg
     1801 caccacatct ttcctcccga cagggaccgc tatgtcagga acgtctcaaa tcttcttgtc
     1861 tccgggggca tgtacctatc ggtcagtttc cacgaggacg accctcattt cgggggatca
     1921 ggaaaatatc gcaggacacc aataggaacc accctttact tctcctctgg agaggaactt
     1981 tttgatctct tctccccgca cttcaccatc attgaatcga aggtgcgtac catacccggc
     2041 acttcgggca ctcatgaggc gaactgcttc ctgatggtga aggtctcgta aaaatccacc
     2101 ctgctgcctg cagaccccaa tatgcggaaa ccggcaggtt tatcctgatc ctctcccgat
     2161 tcccatcaca cacgaggtaa aatagtatgg acatcaaggt tctcgggctg aatgc
//
LOCUS       DAOQVJ010000286         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_61536_length_2215_cov_3.586574, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000286 DAOQVJ010000000
VERSION     DAOQVJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61536_length_2215_cov_3.586574"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKL26_06385"
     CDS             complement(<1..190)
                     /locus_tag="PKL26_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNS83356.1"
                     /translation="MSLDEAAQRLAEHLGRQEFVEVYAHHDADGIAAGSILCMALLRR
                     GIHFRLRVLARVSPAAISP"
     gene            complement(197..655)
                     /locus_tag="PKL26_06390"
     CDS             complement(197..655)
                     /locus_tag="PKL26_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNS83357.1"
                     /translation="MARMHARRRGHSGSVRPFRKEAPSWANTDVAAIEKIILDLRKEG
                     VSTSRIGMILRDRYGVPDVKLVTGKRIGQILKEKGMQPEIPEDLRNLIVKALGLRKHL
                     GENKSDLHNKRQLQLTESKVRRLVKYYTGNGRLPKDWSYKPESAEILLSR"
     gene            complement(855..2084)
                     /gene="mmp10"
                     /locus_tag="PKL26_06395"
     CDS             complement(855..2084)
                     /gene="mmp10"
                     /locus_tag="PKL26_06395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107346.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HNS83358.1"
                     /translation="MTHLSVDIGGRPGIDCRGFCEYCYFRHVKESAPLGCRYCPPYKK
                     GCDYCSRSVREYYRGFRDLREIADDVLARLQVIPDEVSRVTISGGGDPSCYPCFTDLV
                     ELLSSMEAPLHIGYTSGKGFDDPGIGRFLVDSGLSEVSFTIFSVQPELRKQYMHDPTP
                     GASLAVLETLCGEIDVYAACVVLPGINDGPILEETCEWLEEKGAKGIILMRFANRTDQ
                     GLVLGNAPVIRGQKVQGVEEFRDMVTDLSARFRIKVSGTPLWDPEIGSPFALAREPDL
                     LKKLPKLERKASIITGRIAAPFIREILSSRGKGARVVPVEKDIACLITIGDLANLDPA
                     TLESLVIIPGRAFVYDREAEEVLSRDNIARTVMRGPDTLTADAETSMGMTRNEVLALE
                     MDAFRDLILQVNRYGST"
BASE COUNT          382 a          672 c          712 g          449 t
ORIGIN      
        1 ccggggatat ggccgccggg ctcacccggg cgaggacccg gagccggaag tggatgcctc
       61 tgcgcaggag agccatgcag aggatggagc ctgccgcgat gccgtccgca tcgtggtggg
      121 cgtagacctc cacgaactcc tgcctgccga ggtgttcggc gagcctctgt gcggcctcgt
      181 caagggacat gggggattat ctcgagagga ggatctcggc gctctccggc ttgtagctcc
      241 agtccttcgg gagccggccg ttcccggtgt agtacttcac caggcggcgg accttggact
      301 cggtgagctg gagctgccgc ttgttgtgga gatcgctctt gttctcgccc aggtgcttcc
      361 ggagcccgag cgcttttaca atcaggttcc gaaggtcctc cgggatctcg ggctgcatcc
      421 ctttctcttt gaggatctgc ccaatccgtt tcccggtgac gagcttgacg tccgggacgc
      481 cgtagcgatc tcggaggatc atcccgatcc tgctggtgga gacgccctcc ttgcgcaggt
      541 ccaggatgat cttctcgatc gccgcgacat cggtgttggc ccaggagggg gcctccttgc
      601 ggaagggccg tacggatccc gagtgccctc tgcgtcgtgc atgcattctt gccattgtgg
      661 tattcacctc cgattacgca atgtaaatcc gaaaagagtc acgctgactc tccgtaatcc
      721 cagaggccgt ggggagaacc ccctccggcc cgtgcgttgc cgcacgtcgg acttacatct
      781 gccagcacta tccaggagtg cttcaatcca tttgggcggg ggaatattaa tgctatcgtg
      841 gacggacagg gcggtcatgt gcttccgtag cggttgacct ggaggatgag gtcccgaaac
      901 gcgtccatct cgagcgccag cacctcgttc cgggtcatcc ccatgctcgt ctcggcgtcc
      961 gcggtgaggg tgtcgggccc ccgcatcacg gtccgggcga tgttgtccct ggagagcact
     1021 tcttctgcct cgcggtcgta gacaaacgcc ctcccgggga tgatcaccag ggattcgagc
     1081 gtggcgggat cgaggtttgc gaggtcgccg atggtgatca ggcaggcaat gtctttttcg
     1141 accggcacca cccttgcgcc cttcccccgc gaggagagga tctccctgat gaacggtgcg
     1201 gcgatcctcc ctgtgattat gctcgctttg cgttcgagtt tagggagttt cttcaggagg
     1261 tcgggctcgc gggcgagtgc aaagggggag ccgatctcag ggtcccagag gggggtgccg
     1321 ctcaccttga tgcggaaccg ggccgagagg tcagtgacca tgtcccggaa ctcctccacc
     1381 ccctgcacct tctgcccgcg gatgacaggg gcgtttccga gcaccaggcc ctggtccgtc
     1441 cggtttgcaa accgcatcag gatgatcccc tttgcaccct tctcctcgag ccattcgcag
     1501 gtctcctcga ggatagggcc gtcgttgatg cccggcagga ccacacaggc ggcgtagacg
     1561 tcgatctccc cgcagagggt ctcgagcacc gcgagcgaag ccccgggcgt ggggtcgtgc
     1621 atgtactgct tccgcagttc cggctgcacg gaaaagatgg tgaacgagac ctcggagagg
     1681 cctgaatcga cgaggaacct tccgatccca ggatcatcga atcccttccc gctcgtatac
     1741 ccgatatgca gcggcgcctc catactggag aggagctcga caaggtcagt gaagcatggg
     1801 tagcagctcg ggtctccgcc gccgctgatg gtgaccctgc tcacctcgtc agggatgacc
     1861 tggaggcggg caaggacgtc atcggcgatc tccctgaggt cccggaaccc ccggtagtac
     1921 tccctcacgc tccggctaca gtaatcgcat ccctttttat agggcgggca gtagcggcag
     1981 ccgagcgggg cagactcctt tacgtgcctg aagtagcagt attcgcaaaa gccccggcag
     2041 tcgatccccg gccgtccccc gatgtccacc gaaaggtgcg tcattcctgg tagagtgtgg
     2101 gtggcagcgg ggcataatgg ctgctatccg gaccatgaca tagggaaggg catatcaggt
     2161 gggaataccc ctttgagaga taagggagcg tgggagtcgt ggaagagatg atcgc
//
LOCUS       DAOQVJ010000287         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_61776_length_2210_cov_2.847332, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000287 DAOQVJ010000000
VERSION     DAOQVJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61776_length_2210_cov_2.847332"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PKL26_06400"
     CDS             <1..884
                     /locus_tag="PKL26_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83359.1"
                     /translation="AMAHFLFNFLGVLIFLPFLGPFTDLVVDLGGSPGQQVANAHLIF
                     NLTCAGIFLVAIKPFSSLVQRVVPGRDDDIVFLPHFLKKPLPDDSKTAVRMVAQEIGH
                     LLETDEEMLKECNHLLDATDTPFQRVQQLREYAQYLDDQIADAVLDLSRRDLERGDAA
                     YYAVLLRVSSLGRVLADEAGDLSDILIQIRERGLKIPADSVLIQKDILSSCERNVGIL
                     RDAFPEISDTVDSAMRSVDETLRVEITRHYHQHVIRISGGKNPAGSTVSRMLFHIEGM
                     AATIREMRKSARMLQVV"
     gene            1049..1687
                     /locus_tag="PKL26_06405"
     CDS             1049..1687
                     /locus_tag="PKL26_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HNS83360.1"
                     /translation="MTMAKKKEKAASAEEPLKLFYIFYNQERWDNWINTLRESNFEPS
                     PQSEEMPEGYTTLYNFSMDITLSVLKIVKLFQNGRYTKEEALEKLNAVEVIVMGEVLQ
                     DNLEEYVESLQLSLLVLFAGCRKFLEGEYKTDIKTLVKEGRKTIDSDMEKALDIAADI
                     AASVINGASCCGKYVKDDIEQPTLFDEWLIEAESMAEAVSSLKNFDEEAGET"
     gene            1684..2136
                     /locus_tag="PKL26_06410"
     CDS             1684..2136
                     /locus_tag="PKL26_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HNS83361.1"
                     /translation="MISQKARFRHARKIERLAGYRLPDQAFHGATLDAISSRLDFDRL
                     DHTLHQQILNFIHSFLRCTCRDAPMCGCPEKKFSREILELRENGLDHRQIAQYLQEEY
                     GIDIYPADILSFLEDSVHVLEAIRDIADLQAMPDLCKNADTHIELLER"
BASE COUNT          517 a          638 c          581 g          474 t
ORIGIN      
        1 ctgccatggc ccacttcctc ttcaactttc tcggggtcct catctttctc ccattcctcg
       61 ggccatttac agaccttgtc gtggacctgg gggggtcacc cggccagcag gtggcaaacg
      121 cccaccttat tttcaacctc acctgcgcag gaatattcct ggtcgcgata aaacccttct
      181 cctctctcgt gcagcgggta gttcccggca gggatgacga catagtcttc ctcccccatt
      241 ttctcaaaaa gcctcttcct gatgattcaa aaaccgcagt ccggatggtc gcgcaggaga
      301 tcggtcacct gttggagaca gacgaagaga tgctgaagga gtgcaaccac cttctcgatg
      361 cgactgatac ccccttccag cgggtgcagc agctcaggga atatgctcaa tacctggacg
      421 accagattgc cgatgcagtc ctcgaccttt cccggcggga tctggagcgt ggcgatgcag
      481 cgtactatgc agtgcttctc agggtctcga gccttggaag agtcctcgca gatgaagcag
      541 gggacttatc tgacatattg atccagatcc gtgagcgggg actgaagatt cccgccgatt
      601 ccgtcctgat ccagaaggac atcctttcat catgcgagcg aaatgtcgga atactccggg
      661 atgcatttcc cgaaatatcc gacacggtcg attctgccat gcggtctgtt gacgagacgc
      721 tcagggttga gatcacgcgg cactatcacc agcacgtcat ccggatctcc ggcggaaaga
      781 accctgcagg gagcacggtc tcccggatgc tcttccacat cgaggggatg gccgcgacca
      841 tccgcgagat gaggaaatcg gcacgcatgc tccaggtggt ctgaaatttt tcagggcggg
      901 ggagatgcgt ccggcctccc cctgcgttct ccaggcagag gattggcctc cactcatttg
      961 cccacccgaa acctcttttc tttacgattc caagggagtt attcccgcac cattccgata
     1021 cacctttaac atacaggaga aaacctgtat gacaatggcg aaaaagaagg aaaaagcggc
     1081 cagtgccgaa gagccgctca aattgttcta tatcttctac aaccaggagc gttgggacaa
     1141 ctggatcaat acccttcgag agtcgaactt cgagcccagt ccacaaagcg aggagatgcc
     1201 cgaagggtac acgactctct acaacttctc gatggacatc acactctcgg tcctgaagat
     1261 tgtcaagctc ttccagaacg gaaggtatac gaaggaggag gccctcgaga agctgaacgc
     1321 agtagaagtg atcgtgatgg gggaggtcct gcaggacaat cttgaggagt atgtcgagtc
     1381 actgcagctc tcgctcctgg tcctgtttgc gggatgcagg aagttccttg agggcgagta
     1441 caagacagat atcaagacgc tcgtcaagga agggaggaag accattgatt cggatatgga
     1501 aaaagcgcta gatattgcag cggatattgc tgcaagcgtg atcaacggtg catcctgctg
     1561 cgggaagtat gtcaaggacg acatcgagca gcccaccctc tttgatgagt ggttgatcga
     1621 ggcagagagc atggccgagg ccgtttcatc actcaagaac tttgatgaag aggccggcga
     1681 gacgtgatat ctcagaaggc ccgtttccgg cacgcacgga aaatcgagcg cctggcgggc
     1741 tatcggctcc ccgaccaggc gttccacggc gccaccctgg atgcgatctc atcacggctc
     1801 gactttgacc ggctggacca taccctccac cagcagatcc tcaacttcat ccactctttc
     1861 ctgcgctgca cctgccgtga cgcccccatg tgcggctgcc cagagaagaa attctcccgc
     1921 gagatcctgg aactccgtga aaacggcctt gaccaccgcc agatcgccca gtatctccag
     1981 gaggaatatg gcatcgacat ctacccggcc gacatcctct ccttcctcga ggactcggtc
     2041 catgtcctcg aggcgatacg tgacatcgct gacctccagg cgatgcctga cctctgcaaa
     2101 aacgcagata cccatatcga actccttgaa cgttgagcac aggctttccc gaatttttta
     2161 taagaacctt catgtccagt gcctgtacat tgacagatac catgggatgt
//
LOCUS       DAOQVJ010000288         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_62244_length_2199_cov_3.662313, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000288 DAOQVJ010000000
VERSION     DAOQVJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62244_length_2199_cov_3.662313"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKL26_06415"
     CDS             complement(<1..481)
                     /locus_tag="PKL26_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS83362.1"
                     /translation="MLNIEDLHVKIGEKEVLHDINLHIKEGETHVLMGPNGSGKTTLL
                     RAIMGFGGLDVTDGHILFKGKDVTRMPIHERAKLGMGMLFQRPPTIAGLKLGKLLAVT
                     SGDRVGMVSEYSRFLRMDTFMDRAINLGFSGGEIKRSEVLQLMVQQPDFVMLDEPESG
                     "
     gene            complement(584..1699)
                     /locus_tag="PKL26_06420"
     CDS             complement(584..1699)
                     /locus_tag="PKL26_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83363.1"
                     /translation="MRPTLAIALLCLLFAAISAPGCLEGLPWIGAPVVYPKIVPVEGG
                     TMPVPPVYTFPFQDRVVTVNIQPDAAVYSGAKAADRSARIYDQSIPDEEWRAGIYRAL
                     MSDPSQEGFFSALVSRFHHERDAYRMDQDEYAELLAVFVQSIPYESQDETSPRFPVET
                     YVEGIGDCDDKSLFLAALLGREGYRTALLYFEPERHMAVGVGCPGPGYQGTGFAYIET
                     TNVSLLGIPPRELSGGTTLSSLPEVIPIGDGSLCYTRCSETEELWKAVEETGRQIALL
                     EADIRIRESALMEKRDQLDAEKASMDRMRSHGNLVGYNMRVPAYNAQVEEYNKMRSGL
                     MEVVERFNHIAGIHNYLVAHPHDRKGAYAWLSALPEW"
     gene            complement(1710..>2199)
                     /locus_tag="PKL26_06425"
     CDS             complement(1710..>2199)
                     /locus_tag="PKL26_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNS83364.1"
                     /translation="EEAGLQVVREGAEVVVVGDAGDAFTYQSMNAAFRLILGGVPLIA
                     LEKDRYWMDTDGLSLSAGPFVGALEYATGTEAELAGKPSAAFFGMALDSMGLTPRECA
                     MIGDDAESDVRGAMDAGMSGVLVKTGKFRADAVSRLERPPTAILPSIAALPGYLQQQA
                     DR"
BASE COUNT          416 a          741 c          609 g          433 t
ORIGIN      
        1 caccgctctc cggctcgtcg agcatcacga agtccggctg ctggaccatc agctggagga
       61 cctcgctcct cttgatctcc ccgcccgaga acccgaggtt gatggcccgg tccatgaagg
      121 tgtccatgcg caggaaccgg ctgtactcgc tgaccatccc cacacggtcc ccggacgtga
      181 cagcgagcag cttcccaagc ttgaggccgg cgatggtggg aggccgctgg aacagcatgc
      241 ccatcccaag tttcgcccgc tcatggatgg gcatccttgt cacgtccttg cccttgaaga
      301 ggatgtgccc gtccgtcacg tcaagccccc cgaaccccat gatcgcccgg agcagggtgg
      361 tctttccgga cccgttcggg cccatcagca catgggtctc cccctccttg atgtggaggt
      421 tgatatcgtg gagtacctcc ttttctccta tttttacatg cagatcctcg atattcagca
      481 tggtatctct ccaaaatttt gagttgcatt attatctcag aggccaagaa ttaaacggtt
      541 gtgaccatgc tcccccggct cccgggaccg cccgccacac ccgtcaccat tccgggaggg
      601 cagagagcca tgcatacgcc cctttccggt cgtgcgggtg ggccacgagg tagttgtgta
      661 tcccggcaat gtggttgaac cgctccacca cctccatcag ccctgacctc atcttgttat
      721 actcctccac ctgtgcgttg tacgcgggga ctcgcatgtt atatcccacc aggttcccat
      781 gtgatcgcat gcggtccatg gacgctttct ccgcatccag ctggtccctt ttctccatca
      841 gtgcactctc cctgatccgg atatctgcct caagaagagc gatctgccgg cccgtctctt
      901 caaccgcctt ccaaagttcc tctgtctcgc tgcagcgtgt atagcagagg gacccgtccc
      961 cgatggggat cacttctggc agggaggaga gggtggtgcc accagagagt tcccgcggag
     1021 ggatacccag caggctcacg ttggtggtct cgatgtaggc gaatcccgta ccctggtacc
     1081 ctgggcccgg gcagcccacc cccacggcca tgtgccgctc cggctcgaag tacaggagcg
     1141 cggtccggta tccttcgcgg ccaagaaggg ccgcaaggaa caggctcttg tcatcgcagt
     1201 ccccgatacc ctccacatat gtctccaccg ggaaacgcgg gcttgtctcg tcctggctct
     1261 cgtaggggat agactggacg aacaccgcca ggagttctgc atattcgtcc tggtccatgc
     1321 ggtaagcgtc ccgctcgtga tgaaaacggg agacaagcgc cgagaagaac ccttcctggg
     1381 atgggtccga cataagcgcc cggtaaatcc cggctctcca ctcctcatcg gggatggact
     1441 ggtcatagat ccgggcgctc ctgtctgcgg cctttgcgcc ggaatacact gcagcatccg
     1501 gctgaatgtt cacggtcacc acacggtcct ggaacggaaa cgtgtacacc ggcgggaccg
     1561 gcatggtacc tccctcaacc ggaacgatct tcggatagac gacaggagcc ccgatccacg
     1621 ggagaccttc caggcaccct ggtgcagaaa ttgccgcaaa gaggagacag aggagagcga
     1681 tggcgagtgt tggccgcatg gctcacgctt cacctgtccg cctgctgctg caggtatccc
     1741 ggaagtgccg caatcgaggg gaggatcgcg gtcggcggcc tctcgagccg cgagaccgcg
     1801 tcggcgcgga acttcccggt cttcacgagg acccctgaca tgcccgcatc catcgcaccc
     1861 cgcacatcgc tttctgcgtc gtcaccgatc atggcgcact cccggggagt gagccccatg
     1921 gagtcaaggg ccatcccaaa gaaagctgcc gagggttttc ccgcaagctc ggcctctgtc
     1981 ccggtcgcat actcaagcgc gccgacaaac ggccctgccg agagcgaaag gccgtccgta
     2041 tccatccagt agcggtcctt ctcgagcgca atcaaaggga ctcccccgag tatgaggcga
     2101 aacgccgcat tcatcgattg ataagtgaag gcatcccctg catcgcccac caccaccacc
     2161 tccgcccctt cccggaccac ctgaagccct gcctcctcg
//
LOCUS       DAOQVJ010000289         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_62614_length_2191_cov_4.042135, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000289 DAOQVJ010000000
VERSION     DAOQVJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62614_length_2191_cov_4.042135"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1267
                     /gene="hemA"
                     /locus_tag="PKL26_06430"
     CDS             23..1267
                     /gene="hemA"
                     /locus_tag="PKL26_06430"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNS83365.1"
                     /translation="MAGVSHHTATVDVLEQARYPDEQAFLEKARDRFRGVMLLQTCNR
                     VEIFVHGEAETLAAFLQEEGRGDFSFCTGADAMRHLLELACGIDSMIVGEDQILGQLK
                     KALQQSRDAGCASPVLELCINKAVHAGTEARRRTHINRGAVSIGSAAVQLAEEQLGTL
                     QGKHILVIGSGEMGMLVAQALAAKDLTAIYVANRTYSRAEMLAKKIGGRAVRLDDLPR
                     YMVQSDVVISCTSAPHPVIHCNTLRDVMKARCWPHDKTPRPLILIDIAQPRDVEEGAD
                     VIAGISLFSIDNLRRINEMTMQARRNEAERAREYLDGELLHFVRLLNSRAADDTLAIL
                     HTWAEAIRLRERDRALARLGTGDLRAREIIDDLSRVLVKKILSDVTVSIRHCAEMGEL
                     ESAESLVSAITRGENLCFRNEE"
     gene            1246..>2191
                     /gene="hemB"
                     /locus_tag="PKL26_06435"
     CDS             1246..>2191
                     /gene="hemB"
                     /locus_tag="PKL26_06435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNS83366.1"
                     /translation="MFPQRRMRRLRGRIVQPLLREQVLQKTDLVAPLFIDETASSRRA
                     IPSMPGQFRYPPREMESVARELWDAGIRAVLLFGVPNEKDAQASGAYDPDGVVQRAVR
                     SIKKSVPGMVVITDVCACEYTDHGHCGIVGDTRSGVDLLNDPSLDIMDRIALSHAGSG
                     ADIVAPSCMLDGVVQSIRHALDREGFEDVLIMSYSTKFASALYGPFREAADSGFQFGD
                     RTTYQIHPGNTREAVMESMLDLDEGADILMVKPAGFYLDILAEIRTLGIPVAAFQVSG
                     EYAMIKAAAGEGWIKEKEVVIESLTCIKRAGADLVITYF"
BASE COUNT          455 a          650 c          695 g          391 t
ORIGIN      
        1 ctgcctcctc accccacttg ccatcgcggg tgtcagccac cacactgcaa cggtggacgt
       61 gctcgagcag gcgaggtatc cggatgagca ggcattcctc gagaaagccc gtgaccgttt
      121 ccgaggggtg atgctcctcc agacctgcaa ccgtgtggag atattcgtcc atggagaagc
      181 cgagacgctg gccgccttcc tccaggaaga agggaggggg gatttctcgt tctgcacggg
      241 agcagacgcc atgcgacacc tcctcgagct tgcctgcggg attgattcca tgattgtggg
      301 tgaggaccag atcctcggcc agctcaagaa ggccctccag cagtcgaggg acgcaggatg
      361 cgcaagtccg gtgctcgagc tctgcatcaa caaggcagtc cacgcaggga cagaggcccg
      421 gaggcggacg catatcaaca ggggcgccgt ctcgatcggt tctgccgcag tgcagctcgc
      481 ggaggagcag ctcggcacgc tccagggcaa gcacatcctc gtgatcgggt caggggagat
      541 gggcatgctt gttgcacagg cgcttgcggc gaaggacctc accgccatct atgtcgcaaa
      601 ccggacctat tcccgggccg agatgctggc aaaaaagatc gggggcaggg cagtgaggct
      661 cgacgacctc ccccgctaca tggtccagtc ggacgtggtc atctcctgca cttcggcacc
      721 gcacccggtc atccactgca ataccctccg cgatgtgatg aaggctcgct gctggccgca
      781 tgacaagaca ccccggcccc tcatcctgat cgacatcgcg caaccaaggg acgtcgagga
      841 gggagcggat gtgattgccg gaatctccct cttctcaatc gacaacctcc ggaggatcaa
      901 cgagatgacc atgcaagcca ggaggaatga agccgagcgg gcaagggagt atctggatgg
      961 cgagctcctc cattttgtaa ggctcctcaa cagccgggcg gctgacgata ccctggccat
     1021 cctgcatacc tgggctgaag ctatccgcct cagggagcgg gacagggcgc ttgcaaggct
     1081 tgggacaggc gaccttcggg caagggagat cattgacgac ctctccaggg tgctcgtcaa
     1141 gaagatcctc tcggatgtga cagtctccat ccgccactgc gcagagatgg gcgaactcga
     1201 gtctgccgag tcgctggtat ccgccatcac cagaggtgag aatctgtgtt tccgcaacga
     1261 agaatgagaa ggctcagggg acgaatcgtc cagcccctcc tccgggagca ggtactgcag
     1321 aagacagacc tcgttgcccc gctgttcatc gacgagaccg catcatcgag gagggcgatt
     1381 ccctcaatgc cgggacagtt ccgttatccc ccacgggaga tggagtctgt cgcaagggag
     1441 ctctgggacg ccgggatccg ggccgtcctg ctctttggag tcccgaatga gaaggatgca
     1501 caggcatcag gtgcatacga tcccgacggg gtggtgcagc gcgctgtcag gtcgataaag
     1561 aagtcggtcc ccgggatggt ggtgatcacg gacgtctgtg cctgcgagta taccgatcac
     1621 ggacactgcg gcatcgtggg cgacacccgt tcaggagtcg accttctgaa cgacccatcg
     1681 ctcgacatca tggaccgcat cgcattgagc catgcaggga gcggggcgga tattgttgca
     1741 ccctcctgca tgctcgacgg cgttgtccag tccatacggc atgcccttga cagggagggg
     1801 ttcgaagacg tcctgatcat gtcgtattcg accaagttcg cgagcgccct ctacggcccg
     1861 ttcagggagg cggcagactc ggggttccag ttcggggacc ggaccaccta ccagatccat
     1921 cccgggaaca cgcgggaggc tgtcatggag tcgatgctcg accttgacga gggggcggac
     1981 atcctcatgg tgaaaccggc agggttctac ctcgatatcc tggcagagat acgaaccctc
     2041 ggcatccctg tcgccgcgtt ccaggtcagc ggggagtatg ccatgataaa ggccgccgca
     2101 ggggaggggt ggatcaaaga gaaggaggtc gtaatcgaga gcctcacctg catcaagcgt
     2161 gcaggggcgg atctcgtcat cacctacttt g
//
LOCUS       DAOQVJ010000290         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_62750_length_2188_cov_2.932021, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000290 DAOQVJ010000000
VERSION     DAOQVJ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62750_length_2188_cov_2.932021"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..820)
                     /locus_tag="PKL26_06440"
     CDS             complement(95..820)
                     /locus_tag="PKL26_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS83367.1"
                     /translation="MFEAGERVLLVGGERTFFVRAGTGSFSTDRGVLDLDALLQVSQG
                     DVITTHTGQEFRALSPRPTDFFSRASRSGAPMLPKDIGLVIAYTGMNNRDTVLDAGTG
                     SGIAAIYFGGIAKAVRTCEIRPEFSRLAEKNIQDAKLPNVDVVCGDVLSEEGLYDVVH
                     FDLTLTPDHVHHAHSLLRPGGYLACYTPFIEHLFTVMDAAGELFTEIQAHECIEREMT
                     RSQRGSRPSTRVCHSGYVTVARK"
     gene            complement(813..1157)
                     /locus_tag="PKL26_06445"
     CDS             complement(813..1157)
                     /locus_tag="PKL26_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HNS83368.1"
                     /translation="MMPGNINPRKMKQMMKQLGMQVEQFEDVRQIVITTGKGRYVFDA
                     AEVAAMTMQGITTYQITGEPRFEAAALEIPDDDVRMVAEQAQVSLDAARDALQKTGGD
                     IAEAIIHLTSHV"
     gene            complement(1081..1644)
                     /locus_tag="PKL26_06450"
     CDS             complement(1081..1644)
                     /locus_tag="PKL26_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNS83369.1"
                     /translation="MSKGSTSSSQDSCVQRARGIADFQFGRGAGRGLFPDDCRFIFSR
                     TGRIRQVTWNGSRLATLRASDGRLTLGMAGAIRLHQALPAPAYRVRIVPEVAEFILKG
                     KNAFCKHVVEADPGIRSGDEVLVVTGDDELLATGAAVLSGREMLVFNYGVAVKVRQGR
                     SAYDAGKYQPQEDEADDEAAGDAGGAV"
     gene            complement(1580..>2188)
                     /locus_tag="PKL26_06455"
     CDS             complement(1580..>2188)
                     /locus_tag="PKL26_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNS83370.1"
                     /translation="RADRLFAESYLFPDEAARTPLRNAQEFATLLNACGRWTRAFVGS
                     SICRGDRGGAYREAEHMLNNHRAVIRELLEYILTTGVRELSHIQWLHVGNRYPDTIVG
                     IGAGMALLRLNMEKPILVMVEQPEDPGLTKVSMRTTERAIGRGIDLQLALTTASAEVG
                     GAGGGHRIAAGAFIPKESEEHFVERVNQLIAGQLCTEGQGNR"
BASE COUNT          412 a          712 c          601 g          463 t
ORIGIN      
        1 aatacttggc cttctcgaat tcagagtcag ccgacaaatg ggttgacttt tacagtcccc
       61 tgatgtatcc tctcctccct tctcccctcc cttcttactt tctcgcaacc gtgacatacc
      121 cgctatggca cacgcgggta gaagggcgcg aacctcgttg acttcgtgtc atctcccgct
      181 cgatgcattc gtgtgcctga atttcggtga ataactctcc tgcggcatcc atcacggtga
      241 agaggtgctc gatgaacgga gtatagcagg caaggtagcc tccggggcgg agaagcgaat
      301 gggcgtggtg cacgtgatcg ggagtaaggg tgagatcgaa gtgaacgacg tcgtaaagcc
      361 cctcctcact caagacatcc ccgcagacca catccacatt cgggagcttc gcgtcctgga
      421 tgttcttctc ggccaggcgg gagaattcgg gccgtatttc gcaggtcctg acagcctttg
      481 cgatgccacc gaaataaatg gcggcaatgc cgctcccggt cccggcatcg agcacagtat
      541 ccctgttatt cattcccgtg taagcgatga caagaccgat gtccttcggg agcatgggag
      601 caccgctccg gctggcccgc gagaagaagt cagtcgggcg gggagaaagt gcacggaatt
      661 cctgccccgt gtgggtagtg atgacatcac cctgcgatac ctgcagcagg gcatcgaggt
      721 cgaggacacc cctgtcggtg gagaacgacc ctgtcccggc ccggacaaaa aatgtcctct
      781 ctcctccgac taaaaggaca cgttcccctg cctcaaacat ggctggtgag atggatgatg
      841 gcctcggcga tatccccgcc cgtcttttgg agggcatcgc gggcggcatc gaggctgacc
      901 tgggcctgct cggccaccat cctgacgtca tcatccggga tttccaaagc ggcggcctcg
      961 aacctcggct ccccggtgat ctggtaggtg gtgatcccct gcatggtcat tgccgcgacc
     1021 tcggcggcat caaagacgta ccttcccttc ccggtggtga tcacgatctg ccgcacgtct
     1081 tcaaactgct ccacctgcat ccccagctgc ttcatcatct gcttcatctt cctggggttg
     1141 atatttcccg gcatcatatg ctgaccttcc ctgacgtact tttaccgcta caccgtaatt
     1201 aaataccagc atctcccggc ccgacaggac ggcagcgccg gtcgcgagca gttcatcatc
     1261 tcccgtgacc acgagcacct cgtccccgct ccggatgccc gggtcggcct cgaccacgtg
     1321 cttgcagaac gcgttctttc ccttcaggat gaactcggca acttccggga ctattcgtac
     1381 ccggtacgcc ggcgcgggaa gggcctggtg cagccggata gcgccggcca tgccgagagt
     1441 gagcctcccg tcagaggccc tcagagtggc aaggcgcgac ccgttccagg tcacctggcg
     1501 gatccggccg gtcctcgaga agatgaacct gcagtcatcg gggaaaagcc ccctccccgc
     1561 ccccctcccg aactggaagt cagcgattcc cctggccctc tgtacacaac tgtcctgcga
     1621 tgagctggtt gaccctttcg acaaagtgct cctccgattc ctttggtatg aatgctcccg
     1681 ccgcaatacg gtgtcctccc ccggctccgc ctacctcggc agacgcggtg gtgagcgcca
     1741 gctgcaggtc tattccccgt cctatcgccc tctccgtggt ccgcatggag accttggtga
     1801 gcccgggatc ttcgggctgc tctaccatca cgaggatggg tttctccatg ttcaggcgta
     1861 agagcgccat cccggcgccg atgcctacaa tggtgtcggg ataccggttc cccacatgaa
     1921 gccactggat gtgcgagagt tcccggacac ctgtagtgag aatgtactca aggagttccc
     1981 tgatcactgc ccggtggttg ttcagcatgt gctccgcttc tctgtatgca ccgccccgat
     2041 cccccctgca gatggaactt cccacaaaag cccgggtcca cctcccgcag gcatttaaaa
     2101 gcgtggcaaa ttcctgcgca ttccgaagcg gcgtccttgc agcctcatcg ggaaagaggt
     2161 agctctcggc gaagagccgg tcggccct
//
LOCUS       DAOQVJ010000291         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_62780_length_2187_cov_3.982176, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000291 DAOQVJ010000000
VERSION     DAOQVJ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62780_length_2187_cov_3.982176"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PKL26_06460"
     CDS             <1..1073
                     /locus_tag="PKL26_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNS83371.1"
                     /translation="FVSSQEDLVRYHVSSGTTGKPTVVGYTERDLENWTASLARGLVS
                     CGLGRGDVIQVSYGYGLFTGGLGLHYGAERIGATVLPTSVGNTERQIELMQDLKATAI
                     ACTPSYLLHMGEVAEKMGVSIKDDTRLKVGILGAEPWSEQMRQRIQDWLGIRAYDIYG
                     TSELSGPMFCECSEQKGFHVWGDLAYVEILDPETLEPLPAGERGEMVITMLQKEALPM
                     VRYRIGDISAIDDSVCACGRTHPRVQRITGRVDDMLIIRGINVFPSQIEYALMSIPEV
                     GQHFQIIVDRKGALDEMLVQVEVKREAFSDKITDLMSIRKKVEHKLRNILNVAVNVEL
                     VEPGSLPRFEGKAKRVIDRRKI"
     gene            1075..1509
                     /locus_tag="PKL26_06465"
     CDS             1075..1509
                     /locus_tag="PKL26_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNS83372.1"
                     /translation="MKESRFVIKQISIFSENKPGRLAAIARALQEEKINILAFSIAEA
                     DGFGVVRALVDKPKKAHEKLGALGFNVAFTDVIAIRMKDEPGGLYETARILGEGHINI
                     EYAYAYSGKDAAVLILRVDNVEAAISAIQKGGGTLLESSLFQ"
     gene            complement(1516..>2187)
                     /locus_tag="PKL26_06470"
     CDS             complement(1516..>2187)
                     /locus_tag="PKL26_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hexulose-6-phosphate
                     synthase/ribonuclease regulator"
                     /protein_id="HNS83373.1"
                     /translation="IREAIDSPTLPREEQVSPDQAIRDILRVVSTPNISDAMHRKGAM
                     AGISPLCRDVKMAGKAVTVQGFAGDWAKPVEAIDVAGQGDVLVIDNQGVTHVACWGEL
                     ATMSAMERGLAGVVIDGAARDVDDIRKMRFPLFSRAVVPNAGEPKGFGEINAEIRCGG
                     QVVRPGDWVAGDESGVVVIPRERAMEIARRAMEVKKNEERVREEIRRGSTLSRVSDLI
                     RWEKK"
BASE COUNT          438 a          661 c          679 g          409 t
ORIGIN      
        1 tgtttgtatc ctcacaggag gaccttgtca ggtaccacgt ctcttcaggg acgacaggga
       61 agcctaccgt agtggggtat accgagaggg accttgagaa ctggacggcc tcgcttgcga
      121 gggggcttgt ctcctgcggc ctcgggaggg gggatgtcat acaggtgagc tacggctacg
      181 gcctctttac cggcgggctt ggcctgcact acggggctga gcggatcggg gcaaccgtgc
      241 ttccgacgag cgtggggaat accgagcgcc agatcgagct gatgcaggat ctcaaggcca
      301 ccgcgatcgc ctgcaccccg tcgtatctcc tgcacatggg agaggtcgcg gagaagatgg
      361 gagtcagcat caaggatgat acccggttga aggtcgggat cctcggcgca gagccatggt
      421 ctgagcagat gaggcagcgg atccaggact ggctcggcat ccgggcctat gatatatacg
      481 gcaccagcga actttcgggc ccgatgttct gcgagtgcag tgagcagaaa ggtttccacg
      541 tctgggggga ccttgcgtac gtcgagatcc tggatcccga aacactggag ccgcttccgg
      601 ccggtgaacg gggggagatg gtgatcacca tgcttcagaa ggaagcgctc cccatggtgc
      661 ggtaccggat cggggatatc agcgccatcg atgactcggt ctgcgcgtgc gggcggaccc
      721 acccgagggt acagcggatt accgggaggg tcgacgacat gctgatcatc cgcgggatca
      781 acgttttccc ctcgcagatc gagtatgcgt tgatgtcaat tccggaggtg gggcagcact
      841 tccagatcat cgtggacagg aaaggcgcgc ttgacgagat gctggtacag gtcgaggtga
      901 agagagaagc gttctcggac aagatcactg acctgatgag catcaggaag aaggttgagc
      961 acaagcttcg gaacatcctg aacgtggcgg tgaacgtcga actggtcgaa ccgggatccc
     1021 tccccaggtt cgagggaaag gcaaagcgcg tgatcgacag gaggaagatc tgaaatgaaa
     1081 gagagcaggt tcgtaatcaa gcagatatcc atattctccg agaacaagcc cggccggctc
     1141 gcagccatcg cccgggcact tcaggaagag aagatcaaca tcctcgcctt ctctatcgcc
     1201 gaggcggacg gcttcggggt ggtgcgggcg ctggtggaca agccgaagaa agcccatgaa
     1261 aaactgggag cgcttgggtt caacgtggcc ttcaccgatg tgattgccat ccggatgaag
     1321 gacgagcccg gcggcctcta cgagaccgcg cggatcctgg gggaggggca catcaacatc
     1381 gagtacgcct atgcctactc gggaaaagat gccgcggtgc tcatcctcag ggtcgacaac
     1441 gtcgaggcgg cgatctctgc gatccagaag ggtgggggga cgctcctcga gagctccctc
     1501 ttccagtaaa ccctcctact tcttctccca ccgtatcagg tccgagacac gcgagagggt
     1561 gctccccctc cgaatctcct ccctgacccg ttcctcgttc ttttttactt ccatcgcccg
     1621 gcgggcaatc tccatcgccc gctcccgcgg gattaccacc actccgctct cgtcccctgc
     1681 cacccagtct ccggggcgga ccacctggcc cccgcacctg atctcggcgt tgatctcccc
     1741 aaaacccttc ggttcacctg cattcgggac cacggcacgg gagaagagcg gaaaccgcat
     1801 cttccggata tcatcaacgt cccgtgcggc cccatcgatc accacccctg caaggcccct
     1861 ctccatcgcg ctcatggtgg cgagctctcc ccagcacgcc acgtgggtta cgccctggtt
     1921 gtcgatcacc agcacgtcgc cctggccggc aacgtcgata gcctcgacgg gcttggccca
     1981 gtccccggcg aatccctgga ccgtcacggc ttttcccgcc attttcacat ccctgcacag
     2041 cggagatatc cccgccatcg cacctttacg gtgcatggca tcggagatat tgggggtgga
     2101 gaccacacgc aggatgtccc ggatcgcctg gtccggggat acctgctcct cgcgggggag
     2161 ggtgggactg tcgattgctt ccctgat
//
LOCUS       DAOQVJ010000292         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_63298_length_2176_cov_4.733145, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000292 DAOQVJ010000000
VERSION     DAOQVJ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63298_length_2176_cov_4.733145"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKL26_06475"
     CDS             complement(<1..211)
                     /locus_tag="PKL26_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83374.1"
                     /translation="MRSYWKCVTFIALLCLLIQGVAAISVGNIAITPSTDLVSGQTQV
                     RSTFIINFPASGGYTFDDANSLQLAS"
     gene            complement(217..1395)
                     /locus_tag="PKL26_06480"
     CDS             complement(217..1395)
                     /locus_tag="PKL26_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HNS83375.1"
                     /translation="MRIFFIGFGQAGGKIVDMFLEQDRKLGRNSFRGIAVNTARTDLM
                     GLKHIDLKDRILIGQTVVKGHGVGTDNATGAKITFDEIDSIISAIDKRGTHDVDAFMI
                     VAGLGGGTGSGGTPVLTRSLKKVYREPVYVLGILPAPEEGRLYSYNAARSLATLVKEA
                     DNTFIFDNSAWKNEGESIKSAFSRLNDEIVRRFGVLFRAGEVGSAGIGEMVVDSSEII
                     NTLRGGGITSVGYAITEVITKKQKKSVGGILYGIKGSLRKKEATEEVLLGEDRTAKVI
                     SLVRRAMLGRLTLPCDFSSTERALVLVAGPPDELDRKGIEKAKSWVEENIAGVEVRGG
                     DYPVNSNFVAAVVVLATVGNAPRIKELMEIARSTRDDAAKIEEKKPLSFEEEIEPLFE
                     "
     gene            1578..>2176
                     /locus_tag="PKL26_06485"
     CDS             1578..>2176
                     /locus_tag="PKL26_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNS83376.1"
                     /translation="MKICIIGGGLTGLTAAYCLGQDHEVDLYEKRLSLGGCVSSYEIG
                     DYSIEEFYHHCFAGDSHLFSLMESLHVADRLEWLKGTTGYYANSSIYPLNSPTEILRY
                     PLLSFSDKIRLTFLTLRSRKIDTGPLDGITARDFVTREIGERAYQSFFEPLLASKFGE
                     RRDEISAAWLISRIAIRSNRGLSGEQLGYLKGGFFTLIDA"
BASE COUNT          468 a          650 c          535 g          523 t
ORIGIN      
        1 cgcttgcaag ctgcaggctg ttcgcatcat cgaacgtata ccctccgctg gcagggaagt
       61 tgatgataaa ggttgacctg acctgggtct gccctgagac aaggtccgtt gagggcgtga
      121 tggcgatgtt accaaccgat attgctgcaa cgccctgtat caaaagacag agaagggcga
      181 tgaatgtcac acacttccaa tagctcctca tgaatatcac tcaaacaatg gttcaatctc
      241 ttcttcaaat gagagaggtt tcttctcctc gatctttgcc gcatcgtccc tcgtggaacg
      301 ggcgatctcc ataagctcct tgatccgggg ggcatttcct accgtggcaa ggacaacgac
      361 cgcggctacg aaattgctgt tcaccgggta atctcccccc cgtacttcga ctcccgcgat
      421 gttctcctct acccagctct tggccttctc gatgcctttc cggtcaagtt catcgggagg
      481 gccggcaacc agcacgagcg cacgttcggt gctgctgaaa tcgcagggga gcgtgaggcg
      541 tccgagcatc gccctccgta caagggagat caccttggcc gtcctgtctt caccgagcag
      601 cacctcctcg gttgcctcct tcttacggag tgaacccttg atgccgtaga gaataccccc
      661 cacggatttc ttctgcttct tcgtgataac ctcagtgatg gcatatccga cagaagtgat
      721 ccctccgcca cgcagagtgt tgatgatctc gcttgagtcg acaaccatct ccccgatacc
      781 ggccgatccc acttctcctg cacgaaagag tactccgaac ctgcggacta tctcgtcatt
      841 caggcgggaa aatgctgact ttatgctctc cccttcgttc ttccaggcgc tgttgtcaaa
      901 gatgaacgtg ttgtctgcct ctttgaccag tgtcgccagg ctccttgcag cgttgtacga
      961 ataaagcctg ccttcctcgg gcgcaggaag gataccgagg acgtacactg gttcccggta
     1021 gaccttcttc agcgacctgg tcagcaccgg tgtgccccca gaccctgtgc ctcctccaag
     1081 ccctgcaaca atcatgaatg catcgacgtc gtgggtgccc cgcttgtcaa tggcgctgat
     1141 gatgctgtcg atctcgtcga atgtgatctt ggccccggtt gcattgtccg tccccacgcc
     1201 atgtcccttg accacagtct gcccgatgag gatacggtct ttcaaatcga tatgcttcag
     1261 acccatgagg tcggtgcggg cggtattgac ggcaatgccc cggaaactgt ttcgtcccag
     1321 cttcctgtcc tgctcgagga acatatccac gatcttcccg cccgcctgac cgaaacctat
     1381 gaagaatatt ctcatgaaaa cgacccacca gcccacaaat tacgcaagat gctcaaattg
     1441 actcaatgaa tatggtttct tccgggagtt caaatacttt ccttcttttc catttgatgc
     1501 aaactgaatt taattgcgat atttggaatc cggagccttt ttcatcccct ttttccaacc
     1561 tatactgagc gccggacatg aagatctgca tcataggcgg aggcctgaca ggccttacag
     1621 cggcctactg cctcggacag gatcacgagg tcgatctcta tgagaaacgc ctctcacttg
     1681 gagggtgcgt ctcatcctac gagatcgggg actattccat cgaggagttc taccatcact
     1741 gctttgcggg agactcacac ctcttctctc tcatggaatc gctccatgtt gcagaccggc
     1801 tggagtggct gaaggggacc accgggtatt acgcgaacag cagcatctat ccactcaact
     1861 cgccaacaga gatactccga tatccgctcc tctctttttc cgacaagata cggctgacat
     1921 tccttacgct gcggtcgcgg aagatcgaca cagggcctct ggatgggatc acagcacgcg
     1981 actttgtcac gagggagatt ggagaacgtg cctaccagtc attcttcgaa cccctgctcg
     2041 ccagcaaatt cggggaacgg agagacgaga tttcagcagc atggctcatc agccggatcg
     2101 ccatccgctc gaaccgtggt ctctcaggag agcagctcgg atacctgaaa gggggcttct
     2161 ttacgctgat cgatgc
//
LOCUS       DAOQVJ010000293         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_63614_length_2170_cov_3.456265, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000293 DAOQVJ010000000
VERSION     DAOQVJ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63614_length_2170_cov_3.456265"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1087
                     /locus_tag="PKL26_06490"
     CDS             125..1087
                     /locus_tag="PKL26_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS83377.1"
                     /translation="MIPAWILDLSTPDIQAMKERRDYHGLTKALRHRDPRVQWKAARA
                     LGDLGEESLDYLIRALETTWNREARLGIIEALGLIGGPRVVSPLVAQMDDRSNEVRWE
                     AAMALGETGDQAAIPALRKALEDEDKYVRYGAALSLKKLSWQPETKRDEAFLRVGLQE
                     WDTLTGPDSYAVEALGNAIRDQDKNVRLEAVKALGALRAREAAPILYRAISDPDEEVR
                     WESVLAAPACGLPLRFLPRALARRPRARKNPIVAGFLNFMLPGMGYMYIGLWWGLLIF
                     QVDVYATLWTFAATGEFISFSVLFPVYLILALHAWYMARQMPDL"
     gene            complement(1284..1574)
                     /locus_tag="PKL26_06495"
     CDS             complement(1284..1574)
                     /locus_tag="PKL26_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83378.1"
                     /translation="MTDKKSDPDSSSQAGNAAGTEEELFDLLIPPGVPRSIISDIIQK
                     FDVELVERKQRLHFANMDGDEREILAFRGPRPLVEKVQDYMFSELKKFIEEK"
     gene            complement(1585..>2170)
                     /locus_tag="PKL26_06500"
     CDS             complement(1585..>2170)
                     /locus_tag="PKL26_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2114 family protein"
                     /protein_id="HNS83379.1"
                     /translation="VSDFALKGQSDVVKRYVNRAHELIDIRLVPPERRRFGRVPVYAD
                     VAKESGVALIGCDAGENGSTLSALQDLGREIYAQHGLNLLNEVIDRVCARMALRLVDV
                     AAEQGHVLPNTSIGFTGRAAISGRKPDYIIEGIRERNFFENPNDRLVFVDDGLARGAA
                     LMGRCMNSLGKPKNPIGGVREGPCIMARRIKIGK"
BASE COUNT          442 a          610 c          622 g          496 t
ORIGIN      
        1 gacgatcctc ccttaccccc tctctttcgg tatttcaatt tttgctgctg tccatacctg
       61 gcagatcatg aaggggatgc cggacatatg accatccgag catcctgcaa ggagggattg
      121 tgaagtgatc ccggcatgga tcctggatct ctcgaccccg gatatccagg caatgaagga
      181 gcggcgggac tatcacgggc ttacgaaggc gctccgtcac cgtgacccga gagtgcaatg
      241 gaaggctgca cgtgcgcttg gggatcttgg ggaggagagc ctggactacc tgatcagggc
      301 actggaaacc acctggaaca gggaagcacg tctcgggatt atcgaggcac tcgggctgat
      361 cggaggcccc agggtggttt cacctctcgt tgcgcagatg gacgacagga gcaacgaggt
      421 gcgctgggaa gcggcaatgg ctctcggaga gacaggcgat caggctgcga tcccggcgct
      481 gcgcaaggca cttgaggacg aggacaagta cgtccgctac ggggcggcgc tctccctcaa
      541 gaaactctcc tggcagccgg agacaaagag ggacgaagcc tttctgcgtg tgggtttgca
      601 ggagtgggat acactgacgg ggcccgactc gtatgcagtg gaagcactgg gaaatgccat
      661 cagggaccag gataaaaatg tcagacttga ggcggtgaag gcgcttggcg cccttcgtgc
      721 aagggaggca gctcctatcc tttaccgggc catcagcgat cctgatgagg aggtccggtg
      781 ggaatccgtg ctggcagcac ctgcctgtgg gctgcccctc aggttcctcc caagggccct
      841 ggcacgccgc ccccgtgcaa ggaagaaccc gatcgtcgcc gggttcctca acttcatgct
      901 ccccgggatg ggatacatgt acattggtct ctggtggggc ctcctgatct tccaggtgga
      961 tgtgtatgcg accctctgga cctttgcagc caccggcgaa ttcatctcat tttccgtcct
     1021 cttcccggtt tatctgatcc ttgcactcca tgcatggtac atggcgaggc agatgcctga
     1081 tctgtgacgc ctaggaggaa ccggatggcg gtcccggcag atgatgcgca agaaaattaa
     1141 aatgtaaacc ccacgtgcca tatcaccatg gagtgcaata accgtagggg attttggagg
     1201 gttcccttca gcggcgagtg cattcagttt gaccctgtcg gattccccga ccggtgtgaa
     1261 aagaatagtg caggagagga gtgttatttc tcttcgatga atttcttcag ttcactgaac
     1321 atgtagtcct ggactttttc aacgagtgga cggggcccgc ggaatgcgag gatctcccgc
     1381 tcgtctccat ccatgttcgc gaaatgaaga cgctgtttcc tttctaccag ctccacatcg
     1441 aatttctgta tgatgtccga gatgatagag cgcggtactc cggggggtat caggagatcg
     1501 aagagttcct cttctgttcc tgctgcgttt ccagcctggc tggatgaatc aggatcactc
     1561 tttttatcgg tcatgtaatt ttcctcattt gcctatcttg atcctccttg ccatgatgca
     1621 gggcccttcc ctcacgcctc cgatggggtt ctttggttta ccgagggagt tcatgcacct
     1681 tcccatcagc gcagctcccc gggcgaggcc atcgtcgacg aaaaccagtc ggtcattcgg
     1741 gttctcaaag aaatttctct ctctgattcc ttcaatgatg taatccggct ttcgcccaga
     1801 gatcgcagct cttccggtga acccaatgct ggtatttggc aacacatgcc cctgttcggc
     1861 cgccacgtcc acgaggcgta gcgccatgcg tgcgcatacg cgatcgatga cctcgttgag
     1921 gaggttcagg ccgtgctggg catagatctc cctgccaaga tcctgcaatg cggagagggt
     1981 gcttccattc tccccggcat cgcagccgat gagggcgacc ccagattctt ttgccacatc
     2041 ggcatacaca gggaccctcc cgaacctccg ccgttcagga ggcacgagcc gtatatcaat
     2101 caattcatgg gcacggttca cataacgttt cacgacgtcg ctctggcctt tcagtgcgaa
     2161 atcgctcacg
//
LOCUS       DAOQVJ010000294         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_64066_length_2160_cov_4.316865, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000294 DAOQVJ010000000
VERSION     DAOQVJ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64066_length_2160_cov_4.316865"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1328)
                     /locus_tag="PKL26_06505"
     CDS             complement(141..1328)
                     /locus_tag="PKL26_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNS83380.1"
                     /translation="MVVREIAPDIFSVGALDFDRRLFDSLIPLPQGTSYNSYLVKGTK
                     KTALIDTVDPTREMDLVTNLVKARVDTIDYLVVNHAEQDHSGSLPMMAELFPKAKVVV
                     NEKCLDMVTHLLDIGSERCMVINDRDTVDLGGKTLEFLITPWVHWPETMLTYAREDRV
                     LFSCDLFGSHMAESTLRVNDWKEVYHTAKRYYAEIMMPFSSSIREHMEKIDTLDIAII
                     APSHGPIHTEPNLILDAYRDWISDKVKNEVVIPYVSMHGSTEKMVQYLTEALIERGIR
                     VLPFNLVVTDIGELAMALVDSATVVIGTPTVIFGPHPQAVYATYLVNMLRPKMRVVSV
                     IGSFGWGGRTVEVLKGMLTRIEPELLDPVYIKGAPGAKDLKDLDNLADAIAKKHREFG
                     ILT"
     gene            complement(1338..1496)
                     /locus_tag="PKL26_06510"
     CDS             complement(1338..1496)
                     /locus_tag="PKL26_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNS83381.1"
                     /translation="MDSYQCMICGHVYDPGRGEPSVNIPAGTDFSDLPGGWRCPVCQA
                     SPEKFRKA"
     gene            1647..>2160
                     /locus_tag="PKL26_06515"
     CDS             1647..>2160
                     /locus_tag="PKL26_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HNS83382.1"
                     /translation="MHAEDDGVMTVARLMALSARTAPKGKGKDSLVCVIAGPGDLAAL
                     AGEMERLSKSLGLSIFTRDAGNVSASQACLIIGVRGQEPVGINCQGCGFPSCDEMVEA
                     TGKVNGTAAPFRGPNCVIKITDLGIAVGSAVKTASIHNVDNRVMYTAGAAALSLGWLP
                     ACTIAYGIPLS"
BASE COUNT          471 a          598 c          598 g          493 t
ORIGIN      
        1 gacttcgaga ggaactcctg gaaactggaa gaatggctca tggcagctct ccggtaaggt
       61 cactttggca agagaccccg tgaacccaac tgggaaaatc cctcatgcct ggtgaatgaa
      121 aaaagggaga tcattctcct ttacgtgaga ataccgaact ccctgtgctt ctttgcgatg
      181 gcatccgcaa ggttgtcgag atcttttaag tcctttgccc ccggtgctcc ctttatgtac
      241 accgggtcca ggagttccgg ctctatgcgg gtgagcattc ccttcaggac ctctacagtc
      301 cttccacccc acccaaaaga acctatcacc gacacaacac gcatcttcgg gcggagcatg
      361 ttgacaaggt acgtggcgta tactgcctga ggatgcggac cgaagatgac ggtaggggtt
      421 ccgattacca ctgttgcgga atcgacgagt gccatggcaa gttccccgat atcagtgaca
      481 acaaggttga acgggagaac acgtattccc cgctcgatca gtgcctccgt caggtactgc
      541 accatcttct cggtgcttcc atgcatgctc acgtatggga tgacgacctc gtttttcacc
      601 ttgtcgctga tccagtcccg gtaggcgtca aggatgaggt ttggttcagt gtggatggga
      661 ccgtggctcg gcgcgattat ggcgatgtca agggtgtcga tcttctccat gtgctcccgg
      721 atgctggaag aaaaaggcat catgatctcg gcataatacc gctttgccgt atggtagacc
      781 tccttccagt cattcacccg aagcgtgctc tctgccatgt gagacccaaa gaggtcacag
      841 gaaaagagca cgcggtcttc ccgggcataa gtgagcatgg tctcgggcca atgcacccag
      901 ggggtgataa ggaactcgag tgtctttccc ccaagatcga cagtgtcgcg atcattgatc
      961 accatgcagc gctcgctgcc gatgtcgaga aggtgcgtga ccatgtcgag gcacttctcg
     1021 ttcacgacga cttttgcctt tggaaagagc tcggccatca tcggcaggga accggaatgg
     1081 tcctgctcgg catggttgac gaccaggtaa tcgatggtgt ccaccctggc cttcacgagg
     1141 tttgtgacaa ggtccatctc ccttgtggga tcgaccgtgt cgatgagggc cgtctttttt
     1201 gtgcccttca cgaggtacga gttgtagctg gtcccctgtg ggagggggat cagactgtcg
     1261 aagaggcgcc tgtcaaagtc aagcgccccc acggagaata tatctggtgc tatctcccgg
     1321 acaaccatac ctaccactca cgccttcctg aacttctcgg gacttgcctg gcagaccggg
     1381 cacctccagc ctcccgggag gtctgaaaag tctgttccgg ccgggatatt cactgacggt
     1441 tctcctctgc cgggatcata gacatgcccg caaatcatgc attgatatga atccattcgc
     1501 tgcttctctc ctgtaacgtt gatgatgaga atataattga cgcattgtat cctggagaat
     1561 ccaccagcat aatgatttcc atgaaggatc ccactttctt ttgaaatatc aatcaactca
     1621 tatccgtggc ggccaaatca catcccatgc atgctgaaga cgacggtgtc atgacggttg
     1681 cccggctgat ggcgctctcc gcccgtactg caccgaaagg caaaggaaaa gactcgctgg
     1741 tgtgtgtcat cgcgggaccc ggagacctcg ctgcccttgc aggagagatg gagcggctct
     1801 caaaaagcct ggggctttct atcttcacca gggatgcggg gaatgtctct gcatcccaag
     1861 cctgcctgat tatcggagtg agaggccagg agcctgtggg catcaactgc cagggatgcg
     1921 ggtttccctc atgcgatgag atggtggagg caaccggcaa agtcaacggg actgctgccc
     1981 cattcagggg gccgaactgc gtaatcaaga ttacagacct tggcattgcg gtcggatccg
     2041 cggtcaagac cgcgtcgatc cacaatgttg acaacagggt gatgtacacg gcaggcgccg
     2101 cagccctgtc cctgggatgg ctccccgcat gcaccatcgc gtatgggatc ccgctttcgg
//
LOCUS       DAOQVJ010000295         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_64552_length_2150_cov_3.323150, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000295 DAOQVJ010000000
VERSION     DAOQVJ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64552_length_2150_cov_3.323150"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKL26_06520"
     CDS             complement(<1..645)
                     /locus_tag="PKL26_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNS83383.1"
                     /translation="MQQITASKKYPNGIQVSWTEEGRDKNDFFSYEDLVDLKINAFDL
                     LENPRSYRINPAGHQIETTMPGCDLNSCEGQIVISRLFDAPRVLSWKVWTEPKLIMQW
                     WGPKGFTAPSCRNDLRVGGTYLYCMRSPDGQDFWSTGIYQEIVKPERIVCTDSFADEK
                     GNIVPATYYGMSPDFPREMLLTVTFREQAGRTRLTLQHTGLPPGEVNDLTRSGWN"
     gene            complement(779..1777)
                     /locus_tag="PKL26_06525"
     CDS             complement(779..1777)
                     /locus_tag="PKL26_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNS83384.1"
                     /translation="MENRIFSFRTVRILLFVAFTKNTLKYLHHLCHKNTMAVPPDLPD
                     APGTVTFVAKAEGRIYAPGSRVWKGLVDPELIRQYMFGAEVVSEWKEGSMITWKGEWQ
                     GRHYEERGHILRIEPERLLQYNHYSPLSGLPDAPEHYHTVTITLSEDGAFTLLLLISD
                     NNPTEASRDHSQKNWEMMLGKLKELLESEVVQRLFSEYENAFSRLDVGRSAEFFADTF
                     LSAGPQGVVAQSKAEFIRLAGQAAEFIRLAGQAAEFYKAVGQTSARILSLEENRISDE
                     YSLVKVHWGVTFRKTGDRLIEFDVSYLVQKTGPEPKIILFVAHQDEEKAMKELGLL"
     gene            complement(1921..>2150)
                     /locus_tag="PKL26_06530"
     CDS             complement(1921..>2150)
                     /locus_tag="PKL26_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83385.1"
                     /translation="SILTRGGLAGTVGVVVTVVVMTGVIVGVEVRVGGWVPPGPAGSV
                     VHPPRRTINRRSSIKRRDTVFFITAPSFLFL"
BASE COUNT          479 a          601 c          552 g          518 t
ORIGIN      
        1 attccacccg ctccttgtca ggtcattcac ctcaccgggg ggaagacccg tatgctggag
       61 ggtaagccgg gtcctgcccg cctgctccct gaacgtgacg gtcaggagca tctcacgagg
      121 aaaatcaggg ctcatgccat agtaagtagc cggcacgata ttccctttct catcggcaaa
      181 gctgtccgtg cagacgatac gttccggttt cacaatctcc tggtagatcc cggtgctcca
      241 gaaatcctgg ccatccggag accgcataca atagagatag gtcccaccca ccctgagatc
      301 attcctgcag gagggagccg taaacccctt cggtccccac cattgcatga tcaacttcgg
      361 ctcggtccat actttccatg aaagtacccg cggggcatcg aagagacgtg agatgacgat
      421 ctgtccctcg caggaattca ggtcacatcc aggcatggtt gtctctatct gatggccggc
      481 aggattgata cgataggatc ggggattttc caggagatcg aacgcgttga tcttcaggtc
      541 gaccaggtcc tcgtacgaga agaagtcgtt cttatctctc ccttcttcgg tccaggacac
      601 ctggatcccg ttggggtatt tcttgcttgc ggtaatctgt tgcatcgtcg cccttacctt
      661 tcttcagtga gggagagatg gtcaatcata aagaggtatg gggaattttc agattctgaa
      721 acacaagggt ctcgctgatt tgacatggtt cgatcctaat tgtaatgcaa ggccagtttt
      781 agaggagccc gagctccttc atcgccttct cttcgtcctg gtgggcaaca aagaggatga
      841 tcttcggttc tgggccggtc ttctggacga gataagagac gtcaaattcg atcaaccggt
      901 ccccggtctt cctgaacgta accccccaat ggacctttac cagcgagtac tcgtcactta
      961 tccggttctc ctcgagtgac aggatccgtg ccgatgtctg cccgactgcc ttgtagaact
     1021 cggccgcctg gcctgcgagc ctgatgaact cggccgcctg gcctgcgagc ctgatgaact
     1081 cggccttgct ctgtgcaacg acaccctgcg gacctgccga gaggaaggta tcagcaaaga
     1141 attcggcact cctcccgaca tcaagccggg agaatgcatt ctcatactcg gagaagaggc
     1201 gttgtaccac ctcactttca agaagctcct tcagtttccc aagcatcatt tcccagttct
     1261 tctgcgagtg gtcgcgggat gcttcagtcg ggttgttatc cgagatgagc agaagcaggg
     1321 tgaacgcccc gtcttcagag agggtgatag tcacggtgtg gtaatgctcc ggagcatccg
     1381 ggagccctga aagggggctg tagtgattgt actgcagcag gcgttcgggt tcaatcctga
     1441 ggatgtgccc ccgttcctcg tagtgcctcc cctgccattc tcccttccag gtgatcatgc
     1501 tcccttcctt ccattcggag acgacttcgg ccccgaacat atactgccgg atgagctcag
     1561 gatcgacaag ccctttccag accctgctcc cgggagcata gatccggcct tctgctttcg
     1621 ccacgaacgt aaccgttccc ggagcgtctg gaagatcagg tggtactgcc atagtgttct
     1681 tgtggcagag atggtgcagg tattttaagg tgtttttggt aaatgccaca aacagcagga
     1741 tgcgcaccgt ccggaacgaa aagatcctgt tttccattag agtgtttcat atcctccaat
     1801 gcaacgctgg aatgagtttt cagggtgaaa gaagcccccg aatccatgat gtcctgtatt
     1861 ttcagatttt ataacgacag gaaatacata tcaagggcag ggtgtatggg atttcacctt
     1921 tcacaaaaac agaaaggaag gtgctgtaat gaaaaaaacc gtatctctcc ttttgatact
     1981 gcttctcctg ttgatcgttc ttctgggtgg atgcaccacg gaaccggcag ggccaggggg
     2041 aacccagcca ccgactctga cttcaacacc cactataact ccagtcatta caacgacggt
     2101 taccacaacg ccaaccgtcc cggccaatcc cccccgcgtg agaatactct
//
LOCUS       DAOQVJ010000296         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_64721_length_2146_cov_4.122908, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000296 DAOQVJ010000000
VERSION     DAOQVJ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64721_length_2146_cov_4.122908"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="rplV"
                     /locus_tag="PKL26_06535"
     CDS             <1..231
                     /gene="rplV"
                     /locus_tag="PKL26_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNS83386.1"
                     /translation="DAGRYPKKASHAYIRLLQSVVKNAEYIGLDTEKLEIVHVSANRG
                     RGFRGMFPRAMGRATPKRRETVNIEIVVREVQ"
     gene            231..944
                     /locus_tag="PKL26_06540"
     CDS             231..944
                     /locus_tag="PKL26_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNS83387.1"
                     /translation="MAVERKFVAEGVRKARVEKHLKKELKRAGYGGMDLQRTPLGTQV
                     TIFAEKPGIVIGKGGKVVRQLTQDLATDFAVESPQIEVQQVQNPSFNAQIMAERLANA
                     LERGWYFRKAGQSIIRRVMEAGALGCEVVIAGKLTGARARVQKFSDGYIKHCGEPSNT
                     IVEKGYAVAVKKLGVIGVQVKIIPAGAKIPDHFEVTAKREKPRVAEEIAVTSVGEEVL
                     EEIPEGPDDFNEEKLLGRK"
     gene            946..1149
                     /gene="rpmC"
                     /locus_tag="PKL26_06545"
     CDS             946..1149
                     /gene="rpmC"
                     /locus_tag="PKL26_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNS83388.1"
                     /translation="MAIFRAQEVRQLSDVELQEQMEKLKIELIQHNGKVSAGGSTENP
                     GRIRELRRTIARMMTEANSRRRG"
     gene            1151..1435
                     /locus_tag="PKL26_06550"
     CDS             1151..1435
                     /locus_tag="PKL26_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 1"
                     /protein_id="HNS83389.1"
                     /translation="MISPRNILRHELIGLDVTVVEASNPALTGLSGRIIDETRNTLVI
                     HGDRGKKRVQKHRTRFRVTLPDGVLVEIDGSALVMAPERRISALHKTRGK"
     gene            1435..1761
                     /locus_tag="PKL26_06555"
     CDS             1435..1761
                     /locus_tag="PKL26_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNS83390.1"
                     /translation="MANNIGLSVRQPERECDDANCPFHGTLPVRGQVITGKVVSDRMI
                     GTVVVERAYLHYVGKYNRYEKRNSKIHAHNPPCIQARTGDTVRIAECRPLSKTTTFVV
                     VEVQPS"
     gene            1758..>2146
                     /locus_tag="PKL26_06560"
     CDS             1758..>2146
                     /locus_tag="PKL26_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNS83391.1"
                     /translation="MKAKQSNIPRALSTGSRMTCADNTGAREVQIVSVFGYHGVRRRQ
                     PKMGLGDIATVSVKKGTPDMRRKLVRAVVIRQKKEMRRPSGLRVGFEDNAVVVVDEKN
                     EPRGTEIKGPVAREVAERFPKIGSMAT"
BASE COUNT          519 a          592 c          715 g          320 t
ORIGIN      
        1 gatgcaggac gctatccaaa aaaggcgtcc catgcataca tccggctcct gcagtcagtc
       61 gtaaagaacg ccgagtacat cggcctcgac actgaaaaac tggagatcgt gcatgtctcg
      121 gccaaccgcg gccgcggctt ccggggcatg ttcccgaggg ccatgggcag ggcgaccccc
      181 aagcgcaggg agaccgtgaa catcgaaatc gtggtcaggg aagtgcagta atggcagtag
      241 aaagaaaatt cgtggctgaa ggcgtacgga aggcccgcgt ggagaagcac ttaaagaagg
      301 agctgaagcg ggcggggtac ggcggcatgg acctgcagcg cactcccctg gggacccagg
      361 tcacgatatt cgcggagaaa cccggtatcg tgatcggaaa gggtggaaaa gtcgtcaggc
      421 agcttaccca ggaccttgct accgatttcg cggttgagtc accccagatc gaggtgcagc
      481 aggttcaaaa tcccagtttc aatgcccaga tcatggcaga gaggctggca aacgccctcg
      541 aacgcggctg gtacttcagg aaggccggcc agagcatcat cagacgcgtg atggaggcag
      601 gagcgctcgg gtgcgaagtg gtcatcgccg ggaaactgac cggagcccgc gcccgggtcc
      661 agaagttctc cgatggctac atcaagcact gcggagagcc ctcgaacacc atcgtcgaga
      721 aaggttacgc ggttgcggtc aagaaactgg gcgtcatcgg ggtccaggtc aagatcatcc
      781 ccgccggtgc gaagatcccc gatcacttcg aggtgaccgc aaagagggag aagccccgcg
      841 tagccgagga gattgcagtg acatctgtcg gcgaggaggt cctcgaggag atcccggaag
      901 ggcccgatga cttcaacgag gagaaactgc tggggaggaa gtaggatggc catattccgt
      961 gcacaggaag tgcggcagct ctcggacgtg gagctgcagg agcagatgga gaagctcaag
     1021 atagagctga tccagcacaa cgggaaggtc agcgcagggg gttccactga gaatcccggc
     1081 cggatcaggg aactgcgccg gaccatcgcc cggatgatga ccgaagcgaa cagcaggaga
     1141 cgcgggtaag atgatctccc cccggaacat cctcaggcac gagctcatcg gcctggatgt
     1201 caccgtggtg gaggcgagca accccgcgct gaccggcctc tcggggcgga ttatcgatga
     1261 gaccaggaac acactggtca tccatggtga ccgcggaaag aagagggtcc agaaacacag
     1321 gacccgcttc cgcgtcaccc ttccggatgg cgtcctggtg gagatagacg gctccgcgct
     1381 ggtcatggca ccagagaggc gtatcagcgc attgcataag accagaggga aataatggca
     1441 aacaacatcg gattgagcgt caggcagccc gagagggaat gtgacgacgc aaattgtccg
     1501 tttcacggca ctttaccggt gcgcggccag gtgatcaccg gcaaagtcgt gagcgaccgg
     1561 atgattggaa cggtggtggt agagcgggca tacctgcact acgtgggcaa atacaaccgc
     1621 tacgagaagc ggaacagcaa gatccatgcc cacaaccccc cctgcattca ggcgaggacc
     1681 ggtgataccg tgcgcatcgc agagtgcagg ccgctctcaa agaccaccac ctttgtcgtg
     1741 gtggaggtgc agccatcatg aaggcaaagc agtcgaacat cccgagagca ctctccaccg
     1801 gcagccggat gacctgtgcg gacaacaccg gcgcccggga agtccagatc gtgtcagtgt
     1861 tcgggtatca cggcgtgcgc aggcggcagc ccaagatggg gctcggcgat atcgccaccg
     1921 tgagtgtgaa aaaaggcacc cccgacatgc gccgcaaact cgtgcgggcc gtggtgatcc
     1981 ggcagaagaa ggagatgcgc agaccgagcg ggctgcgggt cggttttgag gacaacgccg
     2041 tcgtcgtggt ggacgagaag aacgagccgc gcgggacgga gatcaagggc ccggtcgcca
     2101 gggaagtggc ggagcggttc ccgaagatcg gctccatggc caccat
//
LOCUS       DAOQVJ010000297         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_64785_length_2145_cov_3.244976, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000297 DAOQVJ010000000
VERSION     DAOQVJ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64785_length_2145_cov_3.244976"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..932
                     /locus_tag="PKL26_06565"
     CDS             255..932
                     /locus_tag="PKL26_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS83392.1"
                     /translation="MKKLMILMVIIGFALAGCATRTGTGAAVGAGVGAAVGAGAGQAI
                     GRDTSSTLIGAGIGAVVGGLTGAAIGRYMDNQEQSMRQAIANSEAATMRREQEIVAGA
                     RKQSVDVLTVTFKSDYLFAVNSSTLLPGAQGELQRVADVLRQYPETNIQIAGHTDSSG
                     STDYNQKLSERRAEAVRNALAGMGVNTSRLTTIGYGETRPIASNATEGGRQQNRRVEV
                     RIVPQTA"
     gene            complement(1186..1854)
                     /locus_tag="PKL26_06570"
     CDS             complement(1186..1854)
                     /locus_tag="PKL26_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNS83393.1"
                     /translation="MDIYLRSTEIIDSDNGRIRETALKLAGGLTGEGPRARALFCFVR
                     DEIHYNVYMLSTFREDFRASTTLERGKGYCVQKAVLLAALARAAGIPSRLAFARIVNH
                     KMPAELKAQTGIEEFPSHGYTQLFVKGRWVSVTPAFDRGLCHRIGVPACDFDGEHDAV
                     LAPVDASGNPYVEYIEKYGPHADLPFEWLHSKIFPIWGNKRPWAEADASRGHIMPLSG
                     YRFP"
BASE COUNT          476 a          643 c          609 g          417 t
ORIGIN      
        1 ccaaaaacct tacaacatcg ccctcctgtc ccggaagatc cgggaagtgc tcgagagcga
       61 ggatccgtga gtcttaaccc aggcggcaag gacctctttc ccgtctctct gatatcccaa
      121 tccctcgcag atccgtcctc gctccccgcg gtataatcat ccatacgcaa ccggcacaga
      181 atgcagttga caagggccgg atccgtcgta gactgttcat aaggatggac aatcgacatg
      241 aagggggtga caggatgaaa aaactgatga tcctgatggt tattatcggt ttcgcacttg
      301 ccggctgcgc gacaagaaca gggacaggtg ccgcggttgg tgccggtgtc ggtgcggcgg
      361 tcggggcagg ggccggtcag gccattggac gcgacacctc ctcgacgctc attggcgcag
      421 gcatcggagc cgtcgtcgga ggcctgacgg gagcggcaat aggcagatac atggacaacc
      481 aggagcagtc aatgcgtcag gccattgcca attccgaggc cgcgacaatg agacgggaac
      541 aggagatcgt tgcgggagcc aggaagcaat ccgttgacgt gctcacggtg acctttaaat
      601 ccgattatct tttcgcggtc aattcctcaa ccctgctccc cggtgcccag ggcgaactgc
      661 aaagggtggc ggacgtgctc aggcagtacc ccgaaacaaa tatccagatc gccgggcata
      721 ccgacagcag cggatcgact gactataacc agaagctctc cgagcggcgc gcggaagcgg
      781 taaggaacgc cctggcgggg atgggtgtca acacgtctcg cctgaccacc atcggttacg
      841 gcgaaacgag acccatcgca agcaatgcta ccgagggcgg ccgccagcaa aaccggcgtg
      901 tagaggtacg catcgtgccg cagaccgcat aaaggccctc tagctacggg aagatcaacc
      961 tgaggccagc ttttttccgt actcctccat tgtccagcag gagcgcagga agggacagcc
     1021 tccgcagaga tctattctgc cgagctcaat gaggtaccgc ttccccgagt cggaactgac
     1081 gttgcgggat gtcatctcgt cgatgcaggg aggtgtcctg gacttgcagc catgcttgtt
     1141 ggtgcacccg atgcaataac cacttttcgg cttgatgaga cccccttacg ggaaccggta
     1201 tcccgacaat ggcatgatgt ggccccggga ggcatcggct tcggcccagg gacgcttgtt
     1261 cccccagatg gggaagatct tactgtgaag ccattcaaag ggcagatcgg cgtggggtcc
     1321 atatttttcg atgtactcca cgtaggggtt gccggaagca tccaccgggg caaggacggc
     1381 atcgtgctcc ccgtcgaaat cgcaggcagg cacaccgatc ctgtggcaca ggcccctgtc
     1441 gaaggccggg gtgacgctca cccacctccc ctttacgaaa agctgggtgt acccgtgact
     1501 gggaaactcc tctatgcccg tctgagcctt gagctccgcg ggcatcttat ggttcacgat
     1561 cctcgcgaag gcaagccggg atggaatacc cgcggcacgg gcgagagctg caagaagaac
     1621 ggccttctgg acacaatagc ccttacctct ctcaagagtc gtgctcgccc tgaaatcttc
     1681 cctgaaggtc gagagcatgt aaacattata gtggatctca tcacggacga agcagaagag
     1741 cgcccgagcc ctcgggcctt cccccgtcaa tcccccggcg agcttgagcg ccgtctcccg
     1801 gatgcgaccg ttatcactgt ctatgatctc cgtggaccgg agataaatat ccatctcacc
     1861 catcacagct ccctaaaaaa ccccaccgac cccccaccct cgatccgtcc tattggtctt
     1921 attcgtccga tatgtcctat tataaaacac ataggaccaa taagacatat cggacctata
     1981 ggactgggac ctataggacg aataaggcac ctgggggcgt ctttacaccg tttccctgtc
     2041 gctcgtgatg acttcatcgg tcagttcgtt ggtgtcgcct tccacggtgg ggaagtgctc
     2101 ctcgaggagc ctgcccgcct cgcctatagc ctctacgagg gcgtc
//
LOCUS       DAOQVJ010000298         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_65204_length_2136_cov_4.953868, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000298 DAOQVJ010000000
VERSION     DAOQVJ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65204_length_2136_cov_4.953868"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKL26_06575"
     CDS             <1..628
                     /locus_tag="PKL26_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNS83394.1"
                     /translation="KAVRYAMRCAEYVEVDIRLSRDRVPVIMHDPTIDRTTNGSGRVA
                     DMTYGELSALDAGEGEHVPSLMEVCSLVRGICGLFVEIKEPGSELEVCKILDKEAPSP
                     LLVVSFHRASLEMVSEMLPGTATGFIYSRPVPDAPTMASRKNIRVILPRSDLLTPELV
                     ADAQSRDIEVVPWTLDDPEQWEKAKKMGVDGFVSDNPCSARQWLEGAG"
     gene            916..1776
                     /locus_tag="PKL26_06580"
     CDS             916..1776
                     /locus_tag="PKL26_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNS83395.1"
                     /translation="MDTSILLLGGVLAVTVILFVSDRVRIDLVAILASLSLAWLGLIS
                     PMEAFSGFASNAVIAMAAVMILGFGIERTGVTSRIANAIIRYAGTSEQRVVATTSMTV
                     GCLSSVLQNIGSAALFLPALRRIGKQTRIPVSRLMMPMGFAAILGGSITMIGSSPLIV
                     LNDLLRQSDAEPFSLFAVTPIGVPLLVAGVLLFAFAGDRILPKKEEKAGKTTVAEIWG
                     SITRSGQRPSLPRRPLWERPGKRRFSRCGTALTYSPFVSCGRSLLHPRVQHGSRKARN
                     LHSSGRRRGS"
     gene            1773..>2136
                     /locus_tag="PKL26_06585"
     CDS             1773..>2136
                     /locus_tag="PKL26_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNS83396.1"
                     /translation="MKFIRESGCIPSEPGSRFKEILDGEGYGFAELIVRSKSSIIGKT
                     MREIRFRQLFSIEPIVHQRGEIETRADFSHMPLAAGDVIVAFGSWDTLRLLAGHKDLL
                     LLTHPEGDAVRHGMAHLAV"
BASE COUNT          429 a          590 c          646 g          471 t
ORIGIN      
        1 caaggcggtc cgttatgcaa tgaggtgcgc ggagtatgtg gaggtggaca tccggctctc
       61 ccgcgacagg gtcccggtga tcatgcatga cccgactatc gaccgtacca cgaacgggag
      121 tggccgtgtt gcggatatga catatggaga actgagcgct cttgacgccg gagaagggga
      181 gcatgtcccc tcgctcatgg aagtctgttc acttgtcagg gggatttgtg ggcttttcgt
      241 ggagatcaag gagccgggaa gcgagctgga ggtatgcaaa atcctggaca aggaggcacc
      301 ttctcctctc ctggtggtct cattccaccg tgcatctctc gagatggtct ccgagatgct
      361 cccagggacc gcgaccggct tcatttattc acgccccgtt cccgacgccc cgacaatggc
      421 atccagaaag aatatccggg taatccttcc gaggtctgac ctcctcaccc cggaacttgt
      481 tgctgatgca cagagcaggg atatcgaggt ggtcccctgg actcttgatg acccggaaca
      541 gtgggagaag gcgaaaaaga tgggtgtgga cgggttcgta tcggacaatc catgcagtgc
      601 aaggcagtgg ctggaagggg ccgggtgagg aggacccaca catccatttt tcctctgaac
      661 tgacagggtg ggctgaatcg ggatccgcga tatactgcaa atgatccccg gacaattgtc
      721 caaacccatg ctgattatcc ccatccttga atcccggacg gatagcggtg gactctgcga
      781 ttgcagtatc aagtggtgcc gggctcgtat tatgagtcgc gaggggatgc aaggtcccgg
      841 ccgaaaagac aaccagcgca tcggcgttct tgggtaatct ttttcctctc ttcggccaca
      901 tctgcctata acgccatgga taccagcatt ctcctccttg ggggagtcct tgcggtcact
      961 gtcatcctct ttgtgagcga ccgggtccgg atcgaccttg ttgccatcct ggcaagcctt
     1021 tcgctggcct ggctcggcct catatcccca atggaagcgt tctctggatt cgcgtcgaat
     1081 gcggtcatcg cgatggcggc ggtgatgata ctcgggttcg ggatagagag gacgggcgtc
     1141 acctcccgta ttgccaatgc aatcatcagg tacgccggga caagcgagca gcgggtcgtt
     1201 gccaccactt cgatgacagt cgggtgtctc tcttcggtcc tgcagaacat aggatccgcc
     1261 gcgctcttcc tgcctgcatt gaggcgcatc ggaaaacaga cccggattcc cgtctcccgt
     1321 ttgatgatgc cgatggggtt tgccgcgata ctcgggggga gcatcacgat gattgggtcc
     1381 agccccctga tcgtgttgaa cgatctcctc cgtcaatctg atgcagaacc gttctccctc
     1441 tttgcggtca cgccgatagg ggtccccctc cttgttgcag gggtgctgct ctttgcattc
     1501 gccggggatc gtattctccc caaaaaggaa gagaaggccg gaaaaacgac cgttgcagag
     1561 atttggggat cgatcacccg gtcaggacag cgaccatccc tccctcgtcg tcccttgtgg
     1621 gaaagacccg ggaagaggcg tttttcaaga tgcggtacgg ccttgaccta ctcgccattc
     1681 gtatcctgcg ggagatcgct gttgcaccct cgcgtgcaac acggttcgag gaaagccagg
     1741 aacttgcatt catcgggccg gaggaggggt tcatgaagtt catcagggaa tccgggtgca
     1801 taccgtcaga acccggaagc aggttcaaag agatcctcga tggcgagggg tatggatttg
     1861 cggagttgat tgtcaggtcg aagtcctcca tcatcggcaa gaccatgcgc gagatcaggt
     1921 tcaggcagtt gttctcgatc gagcctatcg ttcaccagag gggggagatc gagacacgcg
     1981 ctgatttttc gcatatgccc cttgcagcgg gagatgtaat cgtggcgttc gggtcgtggg
     2041 acacactcag gctccttgca gggcacaagg atctcctgct cctcacccat cccgaaggtg
     2101 acgcggtgcg gcacggcatg gcgcacctcg cggtgc
//
LOCUS       DAOQVJ010000299         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_65254_length_2135_cov_3.991346, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000299 DAOQVJ010000000
VERSION     DAOQVJ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65254_length_2135_cov_3.991346"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKL26_06590"
     CDS             complement(<1..316)
                     /locus_tag="PKL26_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS83397.1"
                     /translation="MHRPSYLRLLETGELERRVDAAVALLSSCRVCPRECGINRLHDE
                     AGYCRSGRLPVVSSYGPHFGEEPPLVGRHGSGTIFLAGCNMRCVFCQNYAISQEYCGR
                     EVS"
     gene            507..911
                     /locus_tag="PKL26_06595"
     CDS             507..911
                     /locus_tag="PKL26_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS83398.1"
                     /translation="MVKLTPDMKETFSKVNLFPVATASKNGVPNVAPIAFVIMMDDET
                     LWLADNFMNKTLANVKENPQAAIFVYDSYLKRCFQIKGSIAVKTSGPDYEKMKNMVHE
                     KKPGLPAKSLLVMKVTEVFECNPGANAGKKLL"
     gene            complement(920..2113)
                     /locus_tag="PKL26_06600"
     CDS             complement(920..2113)
                     /locus_tag="PKL26_06600"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNS83399.1"
                     /translation="MGKGTIVMAYSGGLDTSICIPLLKERYGYDRVVTVAVHVGQRDE
                     EIRTATEKGLRLADTHYTIDSRERFVHEYIFPAIRANGSYEGYPMGTALARPLIAEEV
                     AKIAALEGSSAVAHGCTGKGNDQLRFDFVFRMAGLEVIAPIRELNLTRDWEIEYAREH
                     DIPVPVAKDRPWSVDENIWSRSIEGGRLEDPAYHPPEEIYAWSISPEKAPDTPEIVTI
                     DFIKGVPHRLNDVEYAGYDLIQRLNAIAGKHGIGRSNMIEDRILGLTAREIYEHPAAT
                     VLLAAHADLEHLTLTRQELAFKRIVDDKWSELAYMGLVHEPLFHDLNAFIARSQDRVS
                     GRVDVMLYKGCCRVLGRSSQEGIYSGDLVSFDTTTIDQCHAIGVSAYYGIQARLVEQG
                     RKKKD"
BASE COUNT          447 a          629 c          605 g          454 t
ORIGIN      
        1 agctgacctc tctcccgcag tattcctggc tgatggcgta gttctggcag aatacgcacc
       61 gcatgttgca tcctgccagg aagatagtgc cggaaccgtg cctccccacg agagggggct
      121 cctctccaaa atggggacca tacgaggaga cgacggggag gcgtccgctc ctgcagtacc
      181 ccgcttcatc atggagccgg ttgataccgc attcgcgggg gcagactctg caggacgaga
      241 gcagggcaac agcagcgtcc acccgccgtt cgagttcgcc cgtctccaga agtcgcagat
      301 acgagggcct gtgcatagga gttccccacc tgtcaccaca cggcgtgggt taccagttcg
      361 tattcctcac aatatatcag ccattcgctg cattatcctc cattgccgca ggaatttgcc
      421 aaaaagaata tggggagtga ggcacattgg agtattgacg gctattccaa gctgtagtgc
      481 aacacaataa agaggtgaat ctgacaatgg taaaactcac acccgacatg aaagagacct
      541 tttccaaggt gaatctcttc cccgtggcaa ccgcatccaa aaacggtgtc ccgaatgttg
      601 cccccatcgc attcgtgatc atgatggacg atgagacgct ctggctggcg gataatttca
      661 tgaataaaac ccttgccaac gtgaaagaga acccacaggc agcaattttt gtgtacgatt
      721 cctacctgaa gaggtgcttc cagatcaagg gatcaatcgc agtgaagaca tcgggcccgg
      781 attacgagaa gatgaaaaat atggtccacg agaagaaacc ggggcttcct gcaaaatcac
      841 tcctggtgat gaaggtcacc gaggtcttcg agtgcaaccc cggagcgaac gccgggaaga
      901 aactgctcta aaaaaaatat taatccttct tttttcgtcc ctgctccacg agccgtgcct
      961 ggatcccgta gtatgccgag accccgatgg cgtggcactg gtcgatggtg gtggtatcga
     1021 acgagacgag gtcgcccgag taaatgccct cctgggacga gcggccaagc accctgcagc
     1081 accccttgta caacatgacg tccaccctcc ccgatacccg gtcctgtgac cttgcaatga
     1141 aggcgttcag gtcgtgaaag agcggttcgt gcacgagccc catgtaggcg agctcggacc
     1201 acttgtcgtc cactattctc ttgaaggcca gctcctgccg tgtgagggtg aggtgctcca
     1261 ggtcggcatg cgccgcgagg aggactgttg ccgccgggtg ctcgtagatc tcccttgctg
     1321 tcaggccgag gatgcggtcc tcgatcatgt tgctccggcc tatgccgtgc tttccggcaa
     1381 tcgcgttcaa acgctgtatc aggtcgtagc ctgcatattc cacgtcgttc aggcggtggg
     1441 ggacaccctt gatgaaatcg atcgtcacga tctcgggagt atcgggtgcc ttctcgggag
     1501 agatgctcca ggcgtagatc tcctcgggcg ggtggtacgc ggggtcctcg agcctccccc
     1561 cctcgatact cctgctccag atgttctcgt ccacgctcca gggccggtcc tttgcgaccg
     1621 ggacgggaat atcgtgctcc cttgcatact ctatctccca gtcgcgggtc aggttcagct
     1681 cgcggatggg agcgatgacc tcgagtcccg ccatgcggaa gacaaagtcg aaccggagct
     1741 ggtcgtttcc cttgccggtg cacccgtggg cgaccgcaga agatccctcc aatgcggcga
     1801 tcttcgcgac ctcttcggcg atgagcggtc gggcaagcgc ggtccccatg gggtatccct
     1861 cgtaggaacc attggccctt atggcaggga agatatactc gtggacgaac ctctcgcggg
     1921 aatcgatggt gtagtgcgta tctgcaagcc tcagcccttt ctcggtggcc gtccggatct
     1981 cctcgtcccg ctggcccacg tgcaccgcga cggtgaccac cctgtcgtac ccgtaccgtt
     2041 ccttcaacag ggggatgcag atggaggtgt cgagtccccc ggaatatgcc atgacaatgg
     2101 ttccttttcc cattcgctca ctcctgcgtg agagt
//
LOCUS       DAOQVJ010000300         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_65268_length_2135_cov_3.004808, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000300 DAOQVJ010000000
VERSION     DAOQVJ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65268_length_2135_cov_3.004808"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKL26_06605"
     CDS             complement(<1..323)
                     /locus_tag="PKL26_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNS83400.1"
                     /translation="MPPGTRDFQTPAEKADSLLIEIPLESPLSGNEQEIVVEIGRTSG
                     KNGFFFEVDSDTLRFYVRSLDPGAFRSAVLDLLRGHGEQGSAPARLHQVFGDLFDRID
                     SVKSYG"
     gene            660..1466
                     /locus_tag="PKL26_06610"
     CDS             660..1466
                     /locus_tag="PKL26_06610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS83401.1"
                     /translation="MQCITFDRYIEAFNVLEKYSVEYPFMLDHLLGVADECFPSGLSM
                     LDIGAGTGKFARSFLERCRVPVHGYTAIEPSADHVAQIRENLDGIPVKKEIILGSFTP
                     ATGFGGRFDLVLLSHCTYCFMPDPEPYLLHALDLVDGRGVAVIYHGSPTNFCSILNLV
                     FGDILPRERVTDPTFTSWNVRDILEKHGIPHEVTCLPGALEVKEIFMPGNEHLLDELI
                     TFSLMVESGSLPQKVLGRAREFLRAIACPGMQGPVLNLGVDAITVRKSPY"
     gene            complement(1481..>2135)
                     /locus_tag="PKL26_06615"
     CDS             complement(1481..>2135)
                     /locus_tag="PKL26_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83402.1"
                     /translation="HPQGESLSPSCAMRSPADSSLSALADHEPFMKAAGSGGLPCRDQ
                     CYEIMVDGSGDGHSIALFPGEEVFFAYERGASNNDAEFNAVILALENLPPRAHARIRT
                     DSQVVVWHLSATNPRRRANYIQKKAQIEELIAARHLKVEIQWIPRRENRADRFLKHYI
                     ESLCGARGREPLHRRVRRLEVENLRLKTKLAKAMNLLEKRPVVVRDTGGYCMFHEQQ"
BASE COUNT          417 a          653 c          630 g          435 t
ORIGIN      
        1 ccgtagcttt tcaccgagtc gatccggtcg aacaggtccc cgaacacctg gtgcaggcgg
       61 gcgggagcgc tcccctgctc tccatggccg cggaggagat cgaggacggc gctcctgaat
      121 gccccggggt cgagggatcg gacgtagaaa cggagggtat cactgtcaac ttcgaagaag
      181 aacccgttct tccccgaagt gcggccgatt tccacgacga tttcctgttc attcccggag
      241 aggggggatt cgagtgggat ctctatcagg agagagtccg ccttctcagc gggggtctgg
      301 aagtcccgcg ttcccggcgg catatctggt gtcaccacct ctcactgcct ggccaggtaa
      361 gagccgccct gcatccatac cgaccaacct ccatcgcggg tcccgctggt gttgtcgact
      421 gcgtcgcatc gcgactgtcc ggtggaaggt ccgggatggc aatccctcaa attccatcaa
      481 gtgagagtgg acctgcagaa gagaaatacc tgcagggtat gcaagggagc gaagggaccc
      541 tccccctctt ttcccccagg tgttcagggg cgcttcgaac ctggaccgga ggctcccggc
      601 acgtttccgg gagtcctcag gaacattatt tccgaacgaa tgaaagagac taaagaacca
      661 tgcagtgcat cacgttcgac cgctacatcg aggcattcaa tgtgctcgag aagtactcgg
      721 tcgagtaccc cttcatgctc gaccacctcc tcggcgtcgc tgatgagtgt ttccccagcg
      781 gtctctccat gctcgatatc ggcgccggga cgggaaaatt cgcgaggtct tttctcgaac
      841 gatgcagggt tcctgtccat ggctacaccg cgatcgaacc gtcagcagac catgtcgcgc
      901 agatccggga gaacctggac ggaatcccgg taaaaaaaga gatcatcctc ggttctttca
      961 cccctgcgac cggctttggg gggaggttcg acctcgtcct gctctcccac tgcacttact
     1021 gtttcatgcc tgaccccgaa ccgtacctgc tgcatgcgct tgacctggtg gacggacgcg
     1081 gtgtagcggt catctaccac ggcagcccga cgaacttctg ctcaatcctg aacctggttt
     1141 tcggggacat cctccccagg gagcgggtca ctgaccccac cttcaccagc tggaacgtgc
     1201 gggacatcct ggagaaacac ggcatccccc atgaggtcac ctgccttcca ggtgcattgg
     1261 aagtaaaaga gatcttcatg ccaggcaacg aacacctcct cgacgagctg atcacgttct
     1321 ctctcatggt ggaatcaggc tcccttcccc aaaaggtcct cgggagggcc agagaatttt
     1381 taagggcgat tgcctgcccc ggcatgcaag gcccggtgct gaacctcggg gtagacgcga
     1441 tcacggtgag gaagagtccg tactgacgcc ccaccatctt ctactgctgt tcatggaaca
     1501 tgcagtatcc gcccgtatcc cggaccacta ccggccgttt ttcgagcagg ttcatggctt
     1561 ttgcaagttt tgtcttcagc cggaggttct ccacttcgag gcgcctgacc cgcctgtgga
     1621 ggggttcgcg cccccttgcc ccgcagaggc tctcgatgta gtgcttcagg aaacggtctg
     1681 cccggttctc tctccgcggg atccactgga tctcgacctt caggtgccgg gcagcgatga
     1741 gctcctcgat ctgcgccttc ttctggatat agttcgcacg ccgccgggga tttgtggcag
     1801 agaggtgcca gacgaccacc tggctgtcgg tacggatccg ggcatgggcc ctgggaggga
     1861 ggttctcgag tgccaggatg acggcgttga actcggcgtc attgttggat gccccccgct
     1921 cgtacgcaaa gaagacctcc tcgccaggaa agagggcaat actgtgcccg tcgccgctcc
     1981 cgtccaccat gatctcgtag cactggtccc gacagggcag gcccccggat ccggcggctt
     2041 tcatgaaggg ttcatggtcg gccagagcgg ataaagaaga atcagcagga gaacgcatcg
     2101 cgcaggaggg ggagagggac tcaccctggg ggtga
//
LOCUS       DAOQVJ010000301         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_65303_length_2134_cov_3.792208, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000301 DAOQVJ010000000
VERSION     DAOQVJ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65303_length_2134_cov_3.792208"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PKL26_06620"
     CDS             <1..1257
                     /locus_tag="PKL26_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS83403.1"
                     /translation="GGEVRWQQWNDRAIFGPSGGIIEFQSVGRDITEKKLMESALRES
                     ETNYRTLVESANSIILRFRADGTITFANPYALSFFGFRKDSLVGKNLMETIVPEFDSE
                     GANLREKILDLAVHPERYRTSQNENVTREQKRVWVSWTNTPLFDAEGNVEEILSIGND
                     ITRIKEMEDELQQLNEMLEQKVVERTSELEDANRELEAFSYSVSHDLRAPLRAIDGFS
                     YMIISKYSSQLPGDAIQYLERIRHNISYMGQLIDAILNFSRMSRQPLITQTIFPSAMV
                     KEILDELLASKGERKVEITQNDLPPLTGDPALVRQVFSNLLSNALKFTKIRKTARIEV
                     GSTVRESRSVYYIRDNGIGFDMQYRDKIFGVFQRLHPAMEYEGAGIGLTIVQRIIQRH
                     RGIIWVESEVDKGTTFYFSFEPGPRE"
     gene            1380..1787
                     /locus_tag="PKL26_06625"
     CDS             1380..1787
                     /locus_tag="PKL26_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNS83404.1"
                     /translation="MNPSQQTSLQVLDTLDLLVRHWNRADIPAILLHADPSCSGFGPW
                     DREHVQGHDHFRAFLERDTGRLQGLGLTGLQVEAIGTIAWVSGAFHSSSRDGTLNPEG
                     RFTAVLKGTGHEWVLVHLHLSFPHADLGEKKTR"
     gene            complement(1813..>2134)
                     /locus_tag="PKL26_06630"
     CDS             complement(1813..>2134)
                     /locus_tag="PKL26_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNS83405.1"
                     /translation="GSDNYKILKENYEILRRTLAHDGSPLRVVPLPMPGESEGSVPAS
                     YTNFYIGNGTVLVPTFNDPADRRALKTLRKVFPGRVVVGIDCSEMIRGMGAIHCVSQQ
                     FPSP"
BASE COUNT          501 a          593 c          593 g          447 t
ORIGIN      
        1 ggcggcgagg tacgttggca gcagtggaat gacagggcaa tattcggacc ttccgggggg
       61 atcattgagt tccagtcggt aggacgggat atcaccgaga agaaactgat ggagagcgcc
      121 ctcagggaga gcgagacaaa ttaccggacc ctcgtcgaga gcgccaacag cattatcctc
      181 cggttcagag cggacgggac catcaccttc gcaaacccgt acgcactctc gttctttgga
      241 ttcagaaagg atagtcttgt cgggaaaaac ctgatggaga cgattgtccc cgagttcgac
      301 agtgaaggag caaaccttcg ggagaagatc ctcgacctcg ctgttcaccc ggagcgctac
      361 aggacttccc agaatgagaa tgtcacccgc gaacagaagc gggtatgggt ctcgtggacc
      421 aacacacctc tttttgatgc agaagggaac gtggaagaga tcctctcaat cggaaacgac
      481 atcacgcgta taaaagagat ggaggatgag ctccagcagt tgaacgagat gctcgaacag
      541 aaagtcgttg aaaggacatc ggaactcgag gacgccaacc gggagcttga agctttcagt
      601 tattccgtct ctcacgacct gcgggctccc ctccgcgcaa tagacggctt ttcctatatg
      661 atcatctcaa aatattccag ccagttgccc ggggatgcca tccagtacct tgagcgcatc
      721 cgccataaca tctcctacat ggggcagctg attgacgcca tcctcaactt ttcccgcatg
      781 tcaaggcaac ccctcatcac ccagacaatc tttccctctg cgatggtgaa agagatactg
      841 gacgaactcc tggcctccaa aggagagagg aaagtcgaga tcacgcagaa cgatctcccc
      901 ccactgaccg gcgatcccgc acttgtcagg caggtattct ccaatctcct ttcaaatgcc
      961 ctgaagttca ccaagatacg taaaactgcc agaattgaag taggaagtac agtccgtgag
     1021 agtcgttctg tctactacat ccgtgacaac gggatcgggt tcgacatgca gtacagggac
     1081 aagatcttcg gggtattcca gaggcttcat cccgccatgg aatacgaggg tgcagggata
     1141 ggcctgacca tcgtgcagcg gatcatccag aggcacaggg ggatcatatg ggtggagtcg
     1201 gaagtcgaca agggaacaac cttctatttc agtttcgagc cgggccccag ggagtaaaaa
     1261 accaggggat ctgctggcca gcctgaagag ttcacaagaa tagtcaatcc tttctctcct
     1321 gccgggggat cctggcaggc gatatcattg tattgaatat cagccatacc atatgttgca
     1381 tgaacccgag tcagcaaacc agtctccagg tgctcgatac gctggatctc ctcgtcaggc
     1441 actggaaccg ggccgatatc cctgcaatcc tgttgcatgc agatccctcg tgctcgggat
     1501 tcggtccctg ggacagggag cacgtccagg ggcacgatca tttccgggca tttctcgaga
     1561 gggatacagg ccggcttcag gggcttggac tgacaggcct ccaggtcgag gcgatcggga
     1621 ccatcgcgtg ggtttccggg gcattccatt catcttctcg cgatggcact ctgaaccctg
     1681 aaggccgttt cacggcagtt ctgaagggga ccggccatga atgggtgctg gtccaccttc
     1741 acctctcctt cccgcatgca gacctgggcg aaaagaagac ccggtaagcc ggctccccct
     1801 cacctgcctc tatcagggtg aggggaactg ctggctcacg cagtggatgg cacccattcc
     1861 ccttatcatc tcgctgcagt cgattcccac cacgactctt cccgggaaga ccttcctgag
     1921 tgtcttgagg gcgcgcctgt cggcagggtc attgaacgtc ggaacaagaa ccgttccgtt
     1981 cccgatgtaa aaattggtgt agctggccgg cacggagcct tctgattcgc ctggcatggg
     2041 gagcggcacc acccggaggg gcgatccgtc gtgcgcgagg gtcctgcgca gaatctcgta
     2101 attctctttg agtattttgt agttatcgct cccg
//
LOCUS       DAOQVJ010000302         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_65358_length_2133_cov_3.605871, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000302 DAOQVJ010000000
VERSION     DAOQVJ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65358_length_2133_cov_3.605871"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKL26_06635"
     CDS             complement(<1..695)
                     /locus_tag="PKL26_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNS83406.1"
                     /translation="MNARANSMGEIIDDPLLRERTRVFSGRHDAGRRLGVFLLSLPPT
                     SEPLVLAIPAGGVPVGKEIAKTLGAPFSLAIVRKVRIPGTTESGFGAVAWDGTVLINE
                     RLRDALFLSSSDVERAVSETAANVKERITRFMAGTPMPSVAGKTVILTDDGLASGFTM
                     LAAIRSLRVRGPARVVVAVPTASRSSAELVGREADQLVCLNIRSGRSFAVADAYEEWY
                     DLDDREVLGELAG"
     gene            964..1626
                     /locus_tag="PKL26_06640"
     CDS             964..1626
                     /locus_tag="PKL26_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNS83407.1"
                     /translation="MQNGFIQKEGNLYVPGAENLIDPANDFLRQVSEDVFDLTLIVLD
                     THFKEEYYETQESRQFPIHCEYGSRDWELAIDLKGAPNIRYMMKNNFSMWTNLRKRAL
                     QCTDPVKRRVYKNLFHILDDPHTPSECIPRDEFLKAFFSGSIPAEVEVTLIGVASDYC
                     NRYSMEGWLSRGARVTILQDLTKGIEKETPLVLAEDRYRCYIPESLRAVESTVYLKEM
                     AD"
     gene            complement(1804..>2133)
                     /locus_tag="PKL26_06645"
     CDS             complement(1804..>2133)
                     /locus_tag="PKL26_06645"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNS83408.1"
                     /translation="GEFAGLTFLEIDPQIRCTGEVGIVLLPEYQAFGYGKEILKTYME
                     FGFDSLSLHRVFGKCDEMNHASARLMEQCGMTFEGTIREHVWLRDHWRSTRYYGILAG
                     EYAALNP"
BASE COUNT          513 a          591 c          555 g          474 t
ORIGIN      
        1 atcccggcaa gttcaccgag tacctcacga tcatcgaggt cgtaccactc ctcgtaggca
       61 tccgcgaccg caaacgaccg gccggaccgg atgttcaggc agacgagttg atctgcctcc
      121 cggcctacga gctcagcgga ggaccgcgat gccgtaggga ccgcgaccac gacgcgagcg
      181 ggccctcgta ccctgagact ccggatggcg gcgagcatcg tgaaccccga ggcaagcccg
      241 tcgtcggtca ggatcacggt ctttccggca acactcggca tcggtgttcc agccatgaac
      301 cgcgtgatac gttctttcac atttgcggct gtctcgctca cagccctctc gacatctgaa
      361 ctggagagga agagcgcatc ccgcagccgc tcgttgatca gcaccgtccc atcccatgcc
      421 acggctccga acccggattc agttgtcccg ggtatcctta ccttcctcac tattgcaaga
      481 gagaacgggg cgccgagggt cttggcgatc tcctttccaa cgggcacacc tcccgcagga
      541 attgccagga caagaggttc gctggtggga ggaagcgaga gaaggaatac tcccagtctt
      601 ctccctgcat catggcggcc ggaaaatacg cgggtccgct cccttaggag cgggtcgtcg
      661 atgatctctc ccatggagtt tgctcttgcg ttcatggggg ataaacgtga ggattggatt
      721 gaattcggat gggaatgggt aacaaccctg cccggacctt cacaggtgat tccaaaagct
      781 tctgatcagg tcaccagatc cggtaatcca tcccgtatgc ctcctccctg aaagtcccct
      841 cgggagaaaa ggattggaaa ggcattgatc cctcccaatc aatcatcagg taaatagtac
      901 tcccgataca atacatggat gaaccatatc atgaagcggg tatcaattct tcaaataggc
      961 gacatgcaga acggctttat tcagaaagaa gggaatctgt atgttccggg agcagagaac
     1021 ctgatcgacc ctgcaaatga ttttttgcgt caggtgagcg aggatgtttt tgatctcaca
     1081 ctcatcgtgc tcgatactca cttcaaggag gaatactacg aaacacagga atcccggcaa
     1141 tttcccatcc actgtgaata cggctcgcgg gactgggaac tcgcaatcga tctgaaaggg
     1201 gctccgaata tccgctacat gatgaaaaat aatttcagca tgtggaccaa tctccggaaa
     1261 cgggctctgc agtgtaccga tcctgtaaaa agacgtgtgt ataaaaattt gtttcatata
     1321 ttggatgatc cacatacccc ttcggaatgc attccccggg atgagttcct taaagcgttc
     1381 ttctcgggca gtatcccggc agaggtcgag gtcaccctga ttggtgtggc gtcggactac
     1441 tgtaaccggt attcgatgga ggggtggctc tcccgcggtg cccgtgtcac aatcctccag
     1501 gacttaacaa aagggattga gaaagagacc ccccttgttc ttgccgaaga ccggtaccgg
     1561 tgttatatac cggagagtct acgggccgtc gagagcactg tatacctgaa ggaaatggca
     1621 gattgatgaa cagatacctt ctgccagagg atgggatacg agagctgaca gtgcataagg
     1681 gtctatctcc cggagaggaa atttcgtcag atttcatgtt tatcaccagc aattccttcg
     1741 aaaagtgcca gagcccttca atctcatcga ctatactaat cactttcgca tctataccca
     1801 atttcagggg ttgagtgccg catattcccc ggcaagaatc ccgtaatacc gtgtcgaccg
     1861 ccagtggtcc cggagccaga catgctcccg gattgtgcct tcaaaggtca ttccacactg
     1921 ctccatcagg cgagccgagg catggttcat ttcgtcgcat ttgccgaaca cccggtggag
     1981 agagagcgaa tcgaacccga actccatgta cgttttaagg atctcttttc cgtacccaaa
     2041 tgcctggtat tcagggagga ggacgattcc gacctcccca gtgcacctga tctgtgggtc
     2101 aatctcgagg aatgtgagcc ccgcaaactc acc
//
LOCUS       DAOQVJ010000303         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_65413_length_2132_cov_2.529129, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000303 DAOQVJ010000000
VERSION     DAOQVJ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65413_length_2132_cov_2.529129"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..213
                     /locus_tag="PKL26_06650"
                     /pseudo
     CDS             16..213
                     /locus_tag="PKL26_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     assembly_gap    333..342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <343..725
                     /locus_tag="PKL26_06655"
     CDS             <343..725
                     /locus_tag="PKL26_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNS83409.1"
                     /translation="RLRREEGMDAIARRSLPRIRACRAPLVLVDGIRGDAEVALFRKE
                     FPGFFLVGIDAPFDERLTRLSMRGREDDFSQSAKLVERDGREVGWGLGRALSSADYTL
                     SNAGTLEEFSRAARTLLCRIMEDP"
     gene            722..1495
                     /locus_tag="PKL26_06660"
     CDS             722..1495
                     /locus_tag="PKL26_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNS83410.1"
                     /translation="MKNPVSFSASSKVWEDITWIYGIEEAGFSGWEIVADGNYRLDSP
                     ENVRRINEAISSTHLSITVHAPYADLNLSTLNYPIWRESVRQVCTCIEQASAWTDRVT
                     IHPGYLSPVGKIMPQRAWDQQKQALREIGRVAADHGSLACLENMIGIREFLCRDPGEL
                     FGMTEGIEGIGVTIDIGHAHTVGLVKEFLPHLGKASHMHIHDNCGQHDEHLAIGEGTV
                     DWKKVGPAIAARYAGVLVVEGRSLEEGKKSIKVVQGWFA"
     gene            1492..>2132
                     /locus_tag="PKL26_06665"
     CDS             1492..>2132
                     /locus_tag="PKL26_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS83411.1"
                     /translation="MSGETLHVYFLGTAAALPTPMRNPACIMIKRGRDTLLFDCGEGA
                     QQQMMRARTGFLVDAVFITHWHADHFLGLFGLAQTLSFMGRTEPLTIYGPEWVHEVAE
                     SVRALSRYNVKFAINPVEVRPGSTVRFPGYSVRAFETSHGMAGLGYVLVEDERPGRFN
                     RQRAIDLGVPPGPLFGRLQRGETVRIVKDGEETEVRPGDVMGHEFMGRVVEVGP"
BASE COUNT          383 a          641 c          719 g          379 t
ORIGIN      
        1 tgggggaggt gatctatgaa ggtgatcggg gtcgttgggt ttccggcaag cggtaagggc
       61 gagttctccc gcatcgccgc ggaggagggg gtccccatcg tggtgatggg cgatgtcata
      121 cgagaggcgg tgcaggagga aggattgccg gtcacggacg caaacctcgg cgcggtggca
      181 aaccgcctcc ggcgggagga ggggatggac gccttcttga acgcgcccac gctgcggatg
      241 ttgtaatcca tcacgcggcg ccgctcgggc gcgtcgaatt cgatccgcat gtccggatag
      301 ttctcggcga tcgcgcacag atcgccgtcg cgnnnnnnnn nnaccgcctc cggcgggagg
      361 aggggatgga cgccatcgca cgccgctcgc tcccccgcat ccgtgcctgc agggcgccgc
      421 ttgtcctcgt cgatgggatc aggggggatg cagaggtcgc gctcttcagg aaggagttcc
      481 cggggttctt tctggtcggg atcgatgccc cgttcgacga acgcctcacg cgcctctcca
      541 tgcggggcag ggaggatgat ttctcgcagt ctgcgaagct cgtggagcgc gacgggcggg
      601 aagtcggctg gggccttggg cgggcgctct cttcggcgga ctacactctc tcgaacgccg
      661 ggacactgga agagttctcc cgggctgcac gaacccttct ttgcaggatc atggaggacc
      721 catgaagaac cctgtctcct tctctgcctc gtcaaaggtc tgggaggaca tcacctggat
      781 ttatggcatt gaggaggccg ggttttcagg ctgggagatc gtcgccgacg gaaactaccg
      841 cctcgactcc cctgagaacg tgcgcagaat caatgaggcc atctcctcca cgcacctctc
      901 catcacggtc cacgcaccct acgccgacct gaacctctcg accctcaact accccatctg
      961 gcgggagtcg gtcaggcagg tgtgcacctg catcgagcag gcctcggcat ggaccgaccg
     1021 cgtgaccatc cacccgggat acctctcgcc ggtgggaaag ataatgccgc agcgtgcgtg
     1081 ggaccagcag aaacaggctc tccgggagat cgggcgcgtc gcggcagacc atgggtcgct
     1141 cgcctgcctc gagaacatga tcgggatcag ggagttcctc tgccgcgacc cgggcgagct
     1201 cttcgggatg accgaaggga tcgaggggat aggcgtcacc atcgatatcg ggcacgcaca
     1261 caccgtgggt ctcgtgaaag agttcctccc gcacctcggg aaggcctcgc acatgcatat
     1321 ccatgacaac tgcggccagc atgacgagca cctcgccatc ggggagggaa ctgttgactg
     1381 gaagaaggtg ggtcccgcaa tcgccgcccg ctatgcggga gtcctcgtcg tcgaggggag
     1441 gagcctcgag gaaggcaaaa agagcataaa ggtggtacaa gggtggtttg catgagcggc
     1501 gagacgctcc acgtctactt cctcgggacg gccgcggcac tccccacccc gatgcgcaac
     1561 cctgcctgca tcatgataaa gcggggccgc gataccctcc tctttgattg cggtgagggg
     1621 gcccagcagc agatgatgag ggccaggaca ggcttcctgg tagacgcagt cttcatcacc
     1681 cactggcacg cggaccattt cctggggctg ttcgggctcg cccagacact ctccttcatg
     1741 ggcaggacag agcctctgac catttacggt ccagagtggg tccacgaggt tgcagagtct
     1801 gtccgtgcgc tctcgcggta caatgtaaag tttgccatca accctgtcga ggtccgtcca
     1861 ggatcgaccg tgcggttccc gggctattct gtccgggcat tcgagacttc ccacggcatg
     1921 gcagggctcg ggtatgtcct cgtggaggac gagaggccag ggcggttcaa ccgccagagg
     1981 gcaatcgatc tcggggtccc gcccgggccg ctctttggca ggctccagcg gggtgagacc
     2041 gtccggatcg tgaaggacgg agaggagacc gaggtgcggc ccggcgacgt catgggtcac
     2101 gagttcatgg gccgcgtggt cgaggtgggg cc
//
LOCUS       DAOQVJ010000304         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_66187_length_2116_cov_5.115478, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000304 DAOQVJ010000000
VERSION     DAOQVJ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66187_length_2116_cov_5.115478"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PKL26_06670"
     CDS             complement(<1..1368)
                     /locus_tag="PKL26_06670"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNS83412.1"
                     /translation="MLPGIQRVREFEWQVPVGYVPGMRVPGVFFLSETLAGTLEEGAV
                     QQLANVATMPGIVARSLAMPDIHWGYGFPIGGVAAFSLDEGVISPGGVGFDINCGVRL
                     LATPLREEDLVKKRELVERLFKAVPTGVGGKSTLRLSGHELDRMLLEGSSWAVGAGFG
                     IDEDVRMCEQQGCMKEAETAAVSQKARQRGMPQSGTLGSGNHFLEIQVVKEVYQPEVA
                     RAFGISAGQVCIMIHCGSRGLGHQVCTDHLRTLEAATRKYGITLPDRQLACAPIDSPE
                     GKAYFGAMAASANYAWANRQVIMHYTRKVMEELYGVPYKDMRLVYDVAHNVAKIEEHV
                     VGGTTMKVCVHRKGATRAFGPGAPDLPPAYAGVGQPVLIPGSMGTSSFLLHGTQGAME
                     KTFGSTCHGAGRVMSRTQAKKRLTGKEIREALGQEGIIVRAPSDAAIADEAPEVYKPS
                     MEVVRV"
     gene            complement(1375..1782)
                     /locus_tag="PKL26_06675"
     CDS             complement(1375..1782)
                     /locus_tag="PKL26_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNS83413.1"
                     /translation="MSFEELEHTADARVRVRAGTLEGLFSEAGRALMQVMYGRPGRDG
                     LSRSVFVQGDDLESLMHAFLSEILFITEVDGLVISGAEVRVGEGIVEGVVRGEPFSRD
                     IHNAGRGVKGISYSGLSIVRDKESYILDVIFDV"
     gene            complement(1772..2068)
                     /locus_tag="PKL26_06680"
     CDS             complement(1772..2068)
                     /locus_tag="PKL26_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83414.1"
                     /translation="MYVRPNTLTPIEDIVVNEDVADYIRRRGCDFRICTSCGGPLLLP
                     VSMKPPKATDLQVRTGDHTIFISIHQARYLQSIHRGMLPMFLDQIEDQSPGHEF"
BASE COUNT          396 a          703 c          577 g          440 t
ORIGIN      
        1 cacccggacc acttccatgc ttggtttata gacttcaggt gcctcgtctg caattgccgc
       61 atcgctcggc gccctgacaa taatgccctc ctgcccgaga gcttccctta tctccttccc
      121 ggtgaggcgc ttctttgcct gcgtccggct catcaccctg ccggccccgt ggcaggtgct
      181 cccgaatgtc ttttccatgg caccctgagt gccgtggagg agaaacgatg atgtccccat
      241 gcttcccgga atgagtaccg gctgcccgac ccctgcatat gcagggggga ggtccggtgc
      301 gcccggccca aacgccctgg tcgctccctt cctgtgcacg cagaccttca tcgtggtccc
      361 ccccaccacg tgctcctcga tctttgccac gttgtgggca acgtcgtaga caagtcgcat
      421 atccttgtag ggaaccccgt acagctcctc cattaccttc cgtgtatagt gcatgatcac
      481 ctggcggttg gcccatgcgt agttcgcaga ggctgccatc gccccgaagt atgccttccc
      541 ctcgggtgag tcgatgggtg cacaggccag ttgccgatcc ggaagcgtga tcccgtattt
      601 cctggtggcc gcctccaggg tccggagatg atcggtgcag acctggtggc ccaggccgcg
      661 tgagccgcag tggatcatga tgcagacctg cccggctgaa atgccgaaag ccctggcgac
      721 ttccggttga tagacctctt ttacgacctg tatctccagg aaatggttcc ccgagccgag
      781 cgttccgctc tgcggcattc ccctctggcg ggctttctgg gacaccgctg ccgtttcagc
      841 ctccttcatg cagccctgct gctcgcacat ccggacgtcc tcatcaatac cgaaacccgc
      901 accgaccgcc caggacgacc cttccaggag catgcggtca agttcatggc cggacagccg
      961 gagtgtgctc tttcccccga cgcctgtcgg aaccgctttg aagagtcgtt ccaccagttc
     1021 ccgctttttc acgaggtcct cttcccggag aggggtggcg agcagacgga ccccgcagtt
     1081 gatgtcgaat cccactccgc ccggcgagat caccccctcg tcaagagaga atgccgcaac
     1141 tcccccgatg ggaaacccgt atccccagtg gatatcgggc atcgcaaggg agcgtgccac
     1201 gatgccgggc atggtggcga cattcgcaag ctgctggact gccccctcct cgagggtccc
     1261 tgccagcgtt tccgaaagga agaacacccc cggcacgcgc atccccggga cgtatcccac
     1321 cgggacctgc cattcgaatt ccctcactct ctgtattcct ggcagcatgg tcattcacac
     1381 atcaaagatg acatccagta tataagactc cttatcacgg acaatggaga gacccgagta
     1441 ggagatcccc ttgaccccgc ggccggcatt gtggatatcc cttgagaagg gctcgcccct
     1501 gaccaccccc tcgactatcc cctctccaac gcggacttcc gctcccgaga tcacgagccc
     1561 gtccacctcg gtgataaaga gaatctcgga gaggaacgcg tgcatgagcg actcgaggtc
     1621 atctccctgc acaaagacgg agcgtgacag gccatccctc ccagggcggc cgtacatcac
     1681 ctgcatcagc gcccgcccgg cttcggagaa gagtccttcg agggtccctg ccctcacccg
     1741 gactctcgca tccgcggtat gctcaagttc ctcaaaactc atggcccggc gactggtcct
     1801 cgatctggtc aaggaacatc ggcagcattc ccctgtggat ggattgaagg tagcgtgcct
     1861 ggtggatgga aataaagatg gtgtggtctc ccgtgcggac ctggaggtct gtggctttcg
     1921 ggggtttcat gctgacagga aggaggagcg ggcccccgca ggaggtgcag atccgaaagt
     1981 cgcatcccct cctcctgatg taatctgcca cgtcttcgtt caccacgata tcttctatag
     2041 gggtgagggt attcggtctc acgtacatga ttctcaccac atcgaccgga aacggtaaaa
     2101 tgattatgct gggaac
//
LOCUS       DAOQVJ010000305         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_66248_length_2115_cov_4.337379, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000305 DAOQVJ010000000
VERSION     DAOQVJ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66248_length_2115_cov_4.337379"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1034)
                     /locus_tag="PKL26_06685"
     CDS             complement(54..1034)
                     /locus_tag="PKL26_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83415.1"
                     /translation="MTQSYNLSPVLRELLEFAETSLGTEIQLVRRTDVPPQGVLIDDF
                     TFGTGKHVIAFSSSQLGMLKDYTICRHCLELLAKGCAAQHNEYRVISFSKDCALPACR
                     QVYLDILKDEGTRNLAVWRKKQLVFLLYMLFHEAFSDLPLTLLANIVIARRYPVIRNA
                     QVYFLLKESMRDMHDLVPVKEFLPQRFFVLHNGMYYARDMLLAYVLSEYKLNPVINIP
                     ELQRFRNLDVKEMMSHRWSRSPWYHTKMVGDALSNILKLTVTMDMERDLDAGYFQELF
                     ALSREMLSRWWVMMGMQDWYVWESPGHLKAAVAAQAGMEEAIRQEIFGTE"
     gene            complement(1154..1804)
                     /locus_tag="PKL26_06690"
     CDS             complement(1154..1804)
                     /locus_tag="PKL26_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNS83416.1"
                     /translation="MPPQERPSTREEVILALNEKFAVMRRLYGVKKIGLFGSVARGDE
                     RPESDVDIEVEFESGMDTYANYIGLSLYLDELLVRRVDLVMSRVLASYLRPELGAEFA
                     ALSRDKVYLGQILDEIAYLSHRTKGVSAKDFQKDETLRRAVTRSMEIIGEAAARVSGA
                     CRQDSRGVSWNELEGLRTRLIHPYFSPDWGLVWNVLNVYLPTVEPAIRKLHARLAR"
BASE COUNT          430 a          629 c          621 g          435 t
ORIGIN      
        1 caaccacggg accgggggaa aatccaggca tcctgggtgg tcacctcact ctgtcattcg
       61 gtcccgaata tctcctgcct gatggcctcc tccatgccgg cctgcgcggc aaccgcggct
      121 ttcaggtgcc cggggctctc ccagacatac cagtcctgca tccccatcat cacccaccag
      181 cgggagagca tctcccgcga aagcgcgaag agctcctgga aatatcccgc gtccaggtcg
      241 cgttccatgt ccatggtgac ggtcagcttg aggatgttcg agagtgcatc gcccaccatc
      301 ttggtatggt accatgggga acggctccag cggtggctca tcatctcctt gacgtccagg
      361 ttcctgaacc gctggagctc ggggatgttg atgaccgggt tcagcttata ttcggagagc
      421 acgtaggcga gcagcatgtc ccttgcataa tacatcccgt tatgaaggac aaaaaagcgc
      481 tggggcagaa attccttgac cggcaccagg tcgtgcatgt cacgcatgct ctcttttaag
      541 aggaagtaga cctgtgcgtt ccggatgacg gggtatctcc tggcaatcac gatgtttgcg
      601 aggagggtga gcggaaggtc cgagaatgcc tcgtgaaaga gcatgtagag gaggaagacg
      661 agctgcttct tcctccagac cgcaaggttc cgggtacctt cgtccttgag gatgtcgaga
      721 tagacctgcc tgcaggcggg gagcgcacag tcctttgaga aggagatgac ccggtattca
      781 ttgtgttggg cggcacaccc ctttgcgagc agctccaggc agtggcggca gatggtataa
      841 tccttgagca tcccgagctg ggagcttgag aatgcgatca cgtgcttccc ggtcccgaac
      901 gtgaaatcgt cgataaggac accctggggt ggaacgtcgg tccgcctgac gagctggatc
      961 tcggtcccaa gggacgtttc tgcgaactca aggagttcgc gcaggacagg ggagaggttg
     1021 taggactggg tcatccgtca cttccagcga gaaggcatgc cggaccgggt cgcatctcaa
     1081 ttagatgtaa cttcgtcacc gatgatacat aaacgatacc aaacagaagg atccagccga
     1141 aagaaagggg gtctcacctt gccaggcggg cgtggagttt cctgattgca ggctcaactg
     1201 tggggagata gacattcagc acgttccata cgaggcccca gtcaggggag aaataggggt
     1261 ggatcagccg ggtgcggagg ccctcgagtt cattccagga aaccccgcgc gagtcctgcc
     1321 tgcacgcgcc cgataccctc gccgccgcct ccccgatgat ctccatgctc cgtgtgaccg
     1381 cacgcctgag ggtctcgtct ttctggaagt ccttggccga gacacccttt gtccggtggg
     1441 agagataggc gatctcgtca aggatctggc cgagatacac cttgtcgcgg gagagcgcgg
     1501 caaattcggc cccaagttcc ggacggaggt acgaggcaag gacgcgggac atgacaaggt
     1561 ccaccctcct cactaaaagc tcgtccaggt acagtgagag cccgatgtag tttgcatagg
     1621 tgtccatccc cgactcgaac tcgacctcga tgtccacgtc gctttccggc cgttcatcac
     1681 cgcgggcgac agaaccgaag agccctatct tctttacccc gtaaagcctg cgcatcactg
     1741 cgaacttttc attcagggcg aggattacct cttccctggt tgacggtctc tcctgtggcg
     1801 gcattgtgca cccaagccct ccctctcctc attcctatgc atgccatggc cttatgggta
     1861 tcgtcaatgc atgcggggtg ccacgggaca gggtatgaac cgggccctgg cccggccccc
     1921 gaaccttctt tatcccatca tgccccctct tgatcatgca caccgcagag atcctggcac
     1981 tcgtcctgat catggtactg ctgccagttg cgttctcagg gtgccttatg cagccgggaa
     2041 cggaactggg agaggtcccc atcccttccc ccgcggggct cccctctccc cacggcacgg
     2101 ccaggggagt ggagg
//
LOCUS       DAOQVJ010000306         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_66847_length_2103_cov_3.928711, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000306 DAOQVJ010000000
VERSION     DAOQVJ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66847_length_2103_cov_3.928711"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKL26_06695"
     CDS             <1..883
                     /locus_tag="PKL26_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS83417.1"
                     /translation="ILLSARYGTTVGIEIDAYRIYLRLPSAVKAADVREILLSLDPIH
                     LEGILKLAMKRTALFKWKMVQIAKKFGAIDPDADYERISTHRLVEIFDGTVVQKEAYR
                     ELFSVYMDVPAAAQIVSLIRDGSIDVAVSPLSILGAEGLFASRDQISPPTADQAVIGT
                     LKRRLEQQEVVLACMHCRKWKSRTTVARVPDPLRCPLCGAGLVAVLKPWEEPLVALAK
                     KKKTQNEEERATEQRLLRNANIVLSGGKKAVTALAARGVGPENASRILSTLAEGDGFY
                     REIIKAERNYIRTRRFW"
     gene            complement(884..1438)
                     /locus_tag="PKL26_06700"
     CDS             complement(884..1438)
                     /locus_tag="PKL26_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HNS83418.1"
                     /translation="MVGSRYESLKIENIVASGAIADAIDLADVSKKIEHCELNQKRFP
                     GAVFRMENPKIASLIFSSGKVVLTGIRDQDSLDKGLSMIIAAMKEAGVDTYPEPRVGV
                     TNIVCSYDIGKNINLNKVVITLNLENIEYEPEQFPGLVYRIKDPKIVALLFSSGKIIL
                     TGGKTLDEIRRGLDFLEQKIGGIL"
     gene            1566..>2103
                     /locus_tag="PKL26_06705"
     CDS             1566..>2103
                     /locus_tag="PKL26_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS83419.1"
                     /translation="MEGTTESQGCYEDLCRNFEIHVPKYFNFGFDVIDSWAEKDRNKL
                     AMIWVNQEKEEKKFSFRDMKNHSNQAANILIKYGIQKGDRVLLVLPRVPEWWVFVIAL
                     IKLGAVVCPCPTMLTPKDIRYRLNAGKFKMVITSLEHAEKVDEIAEECPSLSCRFLVD
                     GERPGWISYPHELFFPAPV"
BASE COUNT          469 a          584 c          610 g          440 t
ORIGIN      
        1 catcctcctc tcggcaaggt acgggaccac cgtggggatc gagatcgatg cctatcggat
       61 ctaccttcgc ctcccttcgg cggtgaaggc ggcagacgta cgggagatac tactctctct
      121 cgacccgatt cacctcgagg gcatcctgaa gcttgccatg aaacggacgg cactcttcaa
      181 gtggaagatg gtacagatcg caaagaagtt cggggcgatc gacccggatg cagattacga
      241 gcgaatcagc acccaccggc tcgtcgagat ctttgatgga acggtggtgc agaaggaggc
      301 ctaccgcgag cttttctcgg tgtacatgga tgtcccggcc gcggcacaga tcgtctcgct
      361 gatcagggat gggtccatag atgtggcagt ctcgccattg agcattctcg gggcagaagg
      421 gctgttcgca tccagggacc agatctcccc gcccaccgcg gaccaggcgg tgatcgggac
      481 cctcaagcgc cgactggagc agcaggaggt ggtccttgcc tgcatgcact gccggaagtg
      541 gaagagcagg acaacggtcg caagggtccc tgatcccctt cgctgcccgc tctgcggtgc
      601 agggcttgtt gcggtattaa agccatggga ggagccgctt gttgccctgg caaagaagaa
      661 aaagacgcag aacgaggagg agcgggcgac tgaacaacgg ctcctccgga atgcaaacat
      721 cgtgctatca gggggaaaaa aggcggtgac cgcgcttgcc gcaaggggag tcggccccga
      781 gaatgcctcg cgaatcctct ccaccctcgc ggagggggat gggttttacc gtgagatcat
      841 caaggcggaa cgaaactaca tccgcaccag gcggttctgg tgatcagagg atcccgccga
      901 tcttctgctc gaggaagtcg aggccccgcc tgatctcatc gagggtcttt cccccggtca
      961 ggattatttt tccggacgag aagaggagtg ccacgatctt cgggtccttg atccggtata
     1021 cgagccccgg gaactgctcg ggttcgtact cgatattctc gaggttcagg gtgataacga
     1081 ccttgtttaa gttgatattc ttgccgatat cgtaggagca gacgatattc gtgacgccca
     1141 cccgcggctc cgggtacgta tccacccccg cctctttcat tgccgcgatt atcatggaga
     1201 gccctttgtc aagcgagtcc tggtcgcgga taccggtgag caccaccttg cctgaagaga
     1261 agatgagaga cgcgatcttt gggttttcca tccggaatac cgcgcctggg aaccgtttct
     1321 ggttaagctc gcagtgttcg atcttcttcg atacgtctgc gaggtcgatc gcatcggcaa
     1381 tggctccgga ggccacgata ttctcgatct ttaaggattc gtacctcgaa ccgaccattg
     1441 ctaataacat cccatttccc gcagcataat actaatcgga tacctttatg tcacaacttc
     1501 cgggcatttt cccggagaaa atggacagat tcaaatccgg gaattgagaa ataatatagg
     1561 tttttatgga gggaacaacc gaatcacagg ggtgttacga ggatctctgc aggaactttg
     1621 agatccacgt cccaaaatat ttcaattttg gctttgacgt catcgattca tgggccgaga
     1681 aggaccggaa caagctggca atgatctggg tcaaccagga gaaggaggag aagaagttct
     1741 cgttccgcga catgaagaac cactccaacc aggcggcaaa catccttatc aagtacggca
     1801 tccagaaggg agaccgggtg ctcctcgtac tgccgagggt ccccgaatgg tgggttttcg
     1861 tgatcgcact gatcaagctc ggggcggtgg tctgcccctg cccgaccatg ctcaccccga
     1921 aggacatcag gtaccgcctg aacgcgggga agttcaagat ggtcatcacc agcctcgagc
     1981 atgcagaaaa agtcgacgaa atcgccgagg agtgtccctc cctctcctgc cggttcctcg
     2041 tggacggcga gcgtcccggg tggatcagct acccacacga gctcttcttc cctgcgccgg
     2101 tct
//
LOCUS       DAOQVJ010000307         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_66902_length_2102_cov_4.448950, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000307 DAOQVJ010000000
VERSION     DAOQVJ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66902_length_2102_cov_4.448950"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..896
                     /gene="mtrD"
                     /locus_tag="PKL26_06710"
     CDS             60..896
                     /gene="mtrD"
                     /locus_tag="PKL26_06710"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit D"
                     /protein_id="HNS83420.1"
                     /translation="MSAVGAKPQAGGAMDMPSTIAGIILLLVIIGATYFLVGSGLMFM
                     ALIGIIIGGLAIAFGVHFVPVGGAPAAMGQAPGIATGVAMLAAGAGLAGLFGGAWAAE
                     LGLVVAVLTGAVGGGLMMAITCMFVTFIYVFGMGIPCASGKVAKDPISGDTQAEFKSQ
                     GTEGHGLPFVSYVGGVIGGLLGGGGGTLIYIMLLDVYKEALPGMMQALPAQVLPIAVS
                     LAGIFAIGMFLVNAVLTAYNITGTIEGPHDPKFKRFPRALVASAVASALCGMVAILIV
                     AM"
     gene            906..1754
                     /gene="mtrC"
                     /locus_tag="PKL26_06715"
     CDS             906..1754
                     /gene="mtrC"
                     /locus_tag="PKL26_06715"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit C"
                     /protein_id="HNS83421.1"
                     /translation="MTVKVEVIEGGVPHNTILAAGLVASLVLIYLTYANALTGTQLFS
                     FFGGLGAIAAIVWGSHAIKHLCSYGIGTGVPSAGMMAFGSGVIAMLFATKYGVAAPIV
                     AVVLAAIIGAILGFLSNNVLNMRIPVMIQSLIEMAIIGALVLMGFAAMMSGSFEFSPL
                     TSGTVTILGLSLPSYQASFIGGGLLAVAFMLGGIAIQHPFNACLGPNWTQDRMLTLAQ
                     ECGFLSMIAVAVMSMALISMAAALVSVIVALVGWYYTYAKYIELSKRDAAGWLDSKPI
                     PELEGH"
     gene            1756..2037
                     /gene="mtrB"
                     /locus_tag="PKL26_06720"
     CDS             1756..2037
                     /gene="mtrB"
                     /locus_tag="PKL26_06720"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
                     /protein_id="HNS83422.1"
                     /translation="MYVQVLPEYGLVVDPTIGVVTTAGESLAPVIEKVADMEAVADDI
                     VNMLSGKGNFLASFPNRENALPTAGAATAYWYGLAVGLFIAGVYVFHLM"
BASE COUNT          394 a          636 c          642 g          430 t
ORIGIN      
        1 gtctgggccc gcaagaacta cggaccctat ggaaagcctg ccgaggaggg atcctgaaga
       61 tgagcgctgt tggagcaaaa ccacaggccg gaggggcaat ggacatgccc tccaccatcg
      121 caggcatcat cctcctgctg gtgatcatcg gtgccaccta cttcctggta gggtcaggcc
      181 tgatgttcat ggctctcatc gggatcatca tcggcggact cgccatcgcc ttcggcgtcc
      241 acttcgtccc ggtcggcggc gcacccgctg ccatgggaca ggcacccggt atcgcgaccg
      301 gtgtcgccat gctcgctgcc ggtgcaggac ttgcaggcct cttcgggggc gcatgggccg
      361 cagaactggg cctcgtggtc gcagttctca caggggcggt cggcggcggc ctgatgatgg
      421 ccatcacctg catgttcgtc accttcatct atgtcttcgg catgggtatc ccctgtgcct
      481 cgggaaaagt cgccaaggac cccatcagcg gggataccca ggccgagttc aagtcccagg
      541 gaaccgaggg gcatggcctt ccgttcgtct cctacgtggg tggcgtcatc gggggtctcc
      601 tcggtggcgg tggcgggaca ctcatctaca tcatgctcct ggacgtgtac aaggaggcac
      661 tccccggcat gatgcaggcg ctgccggcac aggtgctccc catcgcggta agccttgcag
      721 gcatattcgc catcgggatg ttcctggtga acgccgtgct caccgcgtac aacatcaccg
      781 ggacgatcga aggcccgcac gaccccaagt tcaagcggtt cccccgggca ctcgtggcct
      841 ctgcagtggc atccgccctc tgcgggatgg tcgcgatcct gatcgtggca atgtgaggtg
      901 cagaaatgac agtaaaggtt gaagtaatcg agggaggcgt tccccacaac accatcctgg
      961 cagcaggcct cgtcgcgtcg ctggtactca tctacctgac gtatgcgaac gcccttaccg
     1021 ggacccagct cttctcgttc ttcgggggac tgggcgcgat tgccgccatt gtatggggaa
     1081 gccacgccat caagcacctg tgcagctacg gtatcggtac cggcgtgcct tccgcaggca
     1141 tgatggcatt cggttccggc gtgatcgcca tgctgtttgc caccaagtac ggggtggccg
     1201 caccgatagt cgcagtcgtc ctggccgcaa tcatcggagc aattctcggg ttcctgtcca
     1261 acaacgtcct gaacatgcgc atcccggtca tgatccagtc gctgatcgag atggcaatca
     1321 tcggtgcact ggtcctgatg ggatttgcag ccatgatgtc cggatcgttt gagttctctc
     1381 cgctgacctc tggcaccgtg accatcctcg ggctctcgct gccgagttac caggcatcct
     1441 tcattggagg aggtctgctc gcagtggcat tcatgctcgg cgggattgca atccagcacc
     1501 cgttcaatgc atgcctcggt ccaaactgga cccaggacag gatgctcacg cttgcacagg
     1561 agtgcgggtt cctctccatg atcgccgtgg cagtgatgtc gatggctctg atcagcatgg
     1621 cggccgcact ggtatccgtc atcgtggcac tcgtgggctg gtactacacc tacgcgaagt
     1681 acatcgagct ctcgaagcgc gatgcagcag gatggctgga ttcaaaaccg ataccggaac
     1741 tggagggaca ctgatatgta cgtccaggtt cttccggaat acggacttgt ggtcgacccg
     1801 acgatcgggg ttgtgaccac cgcgggcgag tcgcttgcac cggtgatcga gaaggtcgcc
     1861 gacatggaag ccgtcgccga cgatatcgtg aacatgctct ccggaaaagg caacttcctg
     1921 gcttcgttcc cgaacaggga gaacgctctt ccgaccgcag gcgcagcaac cgcctactgg
     1981 tacggactgg cagtcggcct cttcatcgca ggggtgtatg tattccatct gatgtgaggt
     2041 gaaagagatg gcagacaaga aatcaccagc aagcggatgg ccgatcgtgc agggagactt
     2101 cc
//
LOCUS       DAOQVJ010000308         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_66987_length_2100_cov_3.512469, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000308 DAOQVJ010000000
VERSION     DAOQVJ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66987_length_2100_cov_3.512469"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1168)
                     /locus_tag="PKL26_06725"
     CDS             complement(2..1168)
                     /locus_tag="PKL26_06725"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNS83423.1"
                     /translation="MYDLSRLRQEFPVLSRCTYLDSASTSQTPKRAVEAMCAYFYEYA
                     ANHGRGSHRLARRTTEEYEQAREALGGFLGADPGHLVFAKNATDAINMVAHGTCWEPG
                     DEVVTTALEHHANLLPWMALQGRGVRVKVLPQRDGMVDPADLQEALTPRTRLVAVTHV
                     TNVLGSVQPVEEMASIAHDAGVRILLDAAQSAGHMPLSVGGFDFVAIPGHKGLLGPQG
                     TGALFVADYSDLSVTCHGGGIVSEVSLSGFTLLPPPARFEPGTPNIPGVIGLGAAIGL
                     VEELGVGEIHHHETSLGIQAHEGLSSIEGVAVYSPRGSPVVSFNIPGMSPDDCAAALD
                     RRKGICVRSGLHCAHPLASSLHPLGTVRASMGCYTTGEEVAMLIETVEEIARSR"
     gene            complement(1328..>2100)
                     /locus_tag="PKL26_06730"
     CDS             complement(1328..>2100)
                     /locus_tag="PKL26_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS83424.1"
                     /translation="AHQQPILLSGTIPACWISALQSRPDRTVLFFHGGGFSLGSTADH
                     LGLCTRIAEEAEARVISIDYRLAPEHPFPIAIEDCINAYLSLIGKGIPAKRIILAGIS
                     AGGTLALSTLLRARERDLPLPAAAVCMSPAVDMNFRGNSIYTNQETDWVTPGRLEYIR
                     EVYLNGHNPSDPLASPIFADLSGLPPLYIQAGGNEVLIDGIQAFVARAEDAKIEVQFD
                     CYDGMFHCWQVFADVVQEGVVAITRIGEYIQSRLIQNS"
BASE COUNT          431 a          618 c          649 g          402 t
ORIGIN      
        1 gttaccggga cctggcaatc tcctccacgg tttcgatgag catggccacc tcttcacccg
       61 tggtatagca ccccatcgag gccctgaccg ttccaagggg gtgaagcgat gacgcgaggg
      121 ggtgggcgca gtgcaacccg ctccggacgc agattccctt cctccggtcg agtgcggccg
      181 cacagtcgtc agggctcatc cccgggatgt tgaacgagac caccggcgac ccgcgggggc
      241 tgtagacggc caccccttca atggatgaga ggccttcgtg ggcctgtatc cccagactgg
      301 tctcgtggtg gtggatctcg ccgaccccaa gctcctccac gagcccgatt gccgcgccca
      361 ggccgattac cccagggatg ttcggggtgc cgggctcgaa ccgtgccggc ggggggagga
      421 gggtgaagcc cgagagcgag acctcactga cgatgccacc gccgtggcag gtgaccgaga
      481 ggtcggagta atcggcgaca aagagcgccc ccgtcccctg ggggccgagc aggcccttgt
      541 gcccggggat ggccacgaag tcgaacccgc ccacggagag cggcatgtgc cccgccgact
      601 gggccgcatc gaggaggatg cgcaccccgg catcgtgcgc gatcgaagcc atctcctcca
      661 caggctggac actcccgagc acgtttgtga cgtgggtgac cgcaacgagc ctcgtgcgag
      721 gggtgagcgc ctcctggagg tcggcgggat cgaccatccc gtcccgctga gggaggacct
      781 tcacccggac ccccctgccc tggagcgcca tccacgggag gaggtttgcg tggtgctcaa
      841 gcgccgttgt gaccacctcg tcccccggct cccagcaggt accgtgggcg accatgttga
      901 tcgcatccgt cgcgttcttc gcaaagacga ggtgcccggg gtccgccccg aggaacccgc
      961 ccagcgcctc gcgggcctgt tcatactcct cggtggtcct tctcgccagg cggtgggaac
     1021 ccctcccgtg gtttgcggcg tattcataga aataggcgca catcgcttcg accgcccttt
     1081 tcggggtctg gctcgtggaa gcggagtcaa ggtaggtgca gcgggagagg accgggaact
     1141 cctgcctgag acgggacagg tcgtacattc tccagagata tagaccctgg acgatgatga
     1201 gaattgctct tcccctcctc cgcaaggtga gcctggaatt gtgagaggag gacccgtatt
     1261 gctgaaaaaa agggtacatg gaaaagggca tgcacttatc aaaaacttgt gagaaaaatg
     1321 tcaaacttca ggagttttga atcaaacgcg attggatata ctccccgatt cgggtaatgg
     1381 caactactcc ttcctgcact acatcggcaa atacctgcca gcaatggaac atgccatcgt
     1441 agcagtcaaa ctgcacctcg attttcgcat cctccgctct ggccacaaat gcctgtattc
     1501 catcgattaa cacctcgttt cccccggcct ggatataaag aggcggaaga cccgaaagat
     1561 ccgcgaaaat tggtgaggct agcggatcag aagggttgtg gccgttcagg tatacttcgc
     1621 gtatgtactc caatcgcccg ggagtcaccc agtcggtctc ctggttggta taaatagaat
     1681 tcccgcggaa attcatgtca actgcaggtg acatgcagac cgcagcagcg ggaaggggga
     1741 gatctctctc acgtgcacgc aataatgtgg agagcgcgag tgtccccccg gcagaaatgc
     1801 ctgcgagaat tatccgtttt gcgggaattc ccttgcctat gagtgataaa taggcattga
     1861 tgcaatcttc gattgctatg ggaaaggggt gttcgggtgc aaggcggtaa tcgatactga
     1921 tcacccgagc ctctgcctct tctgcgatcc gggtgcagag gccgagatga tcggctgtcg
     1981 aacccagaga gaaaccacct ccatggaaga agagtactgt ccggtctggc ctggattgca
     2041 atgcggatat ccagcatgcg ggtatggtcc cggacaacag gattggctgt tggtgggcat
//
LOCUS       DAOQVJ010000309         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_67341_length_2093_cov_3.368008, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000309 DAOQVJ010000000
VERSION     DAOQVJ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67341_length_2093_cov_3.368008"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKL26_06735"
     CDS             complement(<1..606)
                     /locus_tag="PKL26_06735"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNS83425.1"
                     /translation="MQPRLLCGLACLSLAAALLLCGCTTPESPPDIPAAMTPEDLAGF
                     VHEAAAYAGHAGKEPALSEFNRNPGAFTRGDLYVYAYDYHGTLLAHPYQRESVGTNRL
                     DWTDARGLPAIRIGAHVAAHGGGFIAYLYPAPSASGINESSVESYVPKIGYVYPAGGD
                     WWIASGIYFSDLKGAHPGKYPDAVAEMVDLVRRGAAYGRGHG"
     gene            complement(814..1563)
                     /locus_tag="PKL26_06740"
     CDS             complement(814..1563)
                     /locus_tag="PKL26_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS83426.1"
                     /translation="MPEVDFVLVQPLYEGNVGFAARAIKNFGFSRLVLVDPCPLGDDA
                     IARAGHAKDVLESASRLTLDEVFSGSDLVIATTGEVSKSICNPMRMPYYSPREIRSLV
                     EPHRGKVSLLFGRENWGLNNREIRRSDVICTIPTSPDYPILNLSHAVAILAYELADLP
                     RGEYLLAGRTQMEHLYQHIDQYLTLIDHPDFKRNGTMLMMRRILGRANLTAREASTLH
                     GLMRRSEWYIRPDTRAPDDDIEDGTQDIRED"
     gene            complement(1571..>2093)
                     /locus_tag="PKL26_06745"
     CDS             complement(1571..>2093)
                     /locus_tag="PKL26_06745"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HNS83427.1"
                     /translation="VARYGLPLVVEPAGPEAVIAEGFDFLFIPSVLNTQDVRWIVGKH
                     HQWVQQQEIDWERVVPEAYVVLNPDSSVGKVTRSNCSLSPAEVAAFATVADRYFRFPI
                     VYIEYSGMYGDPEVVRAVSEALDSSVLYYGGGINSTERASEMAKYADTIVVGNAIYDQ
                     GIDVLKATVKAVQ"
BASE COUNT          399 a          611 c          674 g          409 t
ORIGIN      
        1 gccatgcccg cgcccatagg ccgcaccgcg ccgaacgagg tccaccatct ctgcaaccgc
       61 gtccggatac ttcccggggt gtgcgccttt gagatccgag aagtagatgc cggacgcaat
      121 ccaccagtcc ccgcccgcag ggtagacgta cccgatcttc gggacgtagg attcaaccga
      181 ggactcgttt atcccgctgg ccgaaggtgc ggggtagaga tacgcgatga acccgccgcc
      241 gtgtgctgcc acgtgcgcac cgatgcgtat ggcaggcagg ccgcgggcgt cagtccagtc
      301 caggcggttc gtcccgacgc tttcccgctg gtaggggtgg gcaaggagcg tcccgtgata
      361 atcgtaggca tacacataga ggtcgccacg ggtaaacgcc ccggggttgc ggttgaattc
      421 ggagagcgcg ggctcttttc cggcatgacc ggcatatgcc gccgcctcat gcacgaaacc
      481 ggcaaggtct tcaggggtca ttgccgcagg aatgtccggg ggagattcag gggtggtgca
      541 cccgcagagg agcaatgcgg cggcgagtga gaggcaggcc agaccacaga gcagccttgg
      601 ttgcatgcgg gccttatttg gagggaactc acataaatgc cccgatttat gtttctttcc
      661 cgcggtgcag gagtcctacg ccttctctta aagaacaagc ccggtatggt gcagggtgcc
      721 gaccggcaat cgactgaacg gatatactgg ttctgtcgtc tgtaagagaa gaaggggacg
      781 gttcccagcg attggatgtg tcgatggaaa agatcagtct tcgcggatgt cctgtgtgcc
      841 gtcctcgata tcgtcatccg gagctcgtgt atccggcctg atgtaccact cggaacgacg
      901 catcagcccg tgcagggtgc ttgcttcccg cgccgtcagg tttgccctcc cgaggatcct
      961 tcgcatcatc agcatcgtcc cgttcctctt gaaatcgggg tggtcgatga gggtgaggta
     1021 ctggtcgata tgctggtaga ggtgctccat ctgggtcctc ccggcgagga gatactctcc
     1081 cctggggagg tcggcaagct cgtatgcgag gatcgccacc gcgtgggaga ggttcaggat
     1141 ggggtaatca ggcgatgtgg ggatggtgca gatgacatcc gagcggcgga tctcgcggtt
     1201 gttcagcccc cagttctccc tcccgaagag aagagagacc ttcccgcggt gcggttccac
     1261 gagtgatcgg atctcccggg gggagtagta gggcatccgc atcgggttgc agatggactt
     1321 gctcacctcg ccggtcgtgg cgatgacgag gtcgcttccc gagaagacct cgtcgagggt
     1381 gagccgggat gcggactcga gcacgtcctt cgcatggcct gcgcgggcga tggcgtcatc
     1441 cccaagaggg cagggatcaa cgaggacgag ccgtgagaac ccgaagttct tgatggcgcg
     1501 ggcggcaaat cccacgttcc cctcgtagag gggctggacc agtacaaaat cgacctctgg
     1561 catccacggc ctactggacg gctttgactg ttgccttcag cacgtcgatg ccctggtcgt
     1621 agatggcgtt cccgactacg atggtgtctg catacttcgc catctccgag gcccgctcgg
     1681 ttgagttgat ccctcccccg taatacagca ctgatgaatc gagcgcctcc gagacggccc
     1741 tgaccacctc ggggtctcca tacatgccgc tgtactcgat gtacacgatg gggaaacgga
     1801 agtagcgatc agcgactgtc gcaaaggcag cgacctctgc gggtgagagg gaacagttgg
     1861 agcgggtgac cttcccaacc gaggaatcgg ggttgaggac gacatacgcc tcgggcacca
     1921 cgcgttccca gtcgatctcc tgctgctgca cccactggtg gtgcttgccc acgatccacc
     1981 tgacgtcctg agtattcaaa acgcttggaa tgaagaggaa gtcaaagccc tccgcgatca
     2041 ctgcttccgg gccggccggc tccactacga gggggaggcc gtatcttgcc acc
//
LOCUS       DAOQVJ010000310         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_68284_length_2075_cov_4.769307, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000310 DAOQVJ010000000
VERSION     DAOQVJ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68284_length_2075_cov_4.769307"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /gene="hypF"
                     /locus_tag="PKL26_06750"
     CDS             <1..1854
                     /gene="hypF"
                     /locus_tag="PKL26_06750"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNS83428.1"
                     /translation="NCGPRYSIIRDLPYDRERTSMDAFPMCPECKDEYHDPSSRRHHA
                     QTIACASCGPKLRLFDGEGNDVSSGDIIAETARILDAGYIVAIRGIGGFHLACIESSA
                     GELKRRLGRSEQPFAVMVRPDVVDRIAILSAEDQMLLESCQRPIVVLGKRDPDAHQDI
                     SNLHTIGCMLPYTGLHHLLFSRLSHPILIMTSANMPGYPMITGIDVAMARMHGHVDFF
                     LTHDRQIVNRCDDSVVRDGFIIRLSRGMAPRRTAIDLGNTSILAVGPELNANVTIYRD
                     GFCTTSPHVGNVRNPQTYAYLQETIENLGRLLGGNYKYIAHDAHPQFLSTRYARELAG
                     EHGAALVPVQHHQAHIAATTTSPCIGIAIDGVGYGEDGTIWGGEIFAGSIPGYERVGH
                     LEVVPMPGGDLATRFPERMLYGILPTPSIAALLSSRGWTDLELSVLDSQISRGINVVP
                     TSSTGRVLDAAAALLGICRERTYDGEPAMKLESFAVGGKARQWDLDYIQVGGKEVFST
                     RALLQKAMGEMERCKDHDQRAMRSIAASFQFNLARGIAGMAIHAAYKRDLPIVALSGG
                     VAYNRVIRETIRNLVTTSGLELRMNPDYPLGDGCISYGQCVYAGVSAGIEK"
BASE COUNT          425 a          613 c          614 g          423 t
ORIGIN      
        1 aactgcggcc cgcggtacag cattatccgc gacctcccct acgacaggga gaggacatcg
       61 atggacgcct ttcccatgtg cccggaatgc aaggatgagt accatgaccc ttccagccgg
      121 cggcatcatg cccagacgat cgcatgtgcc tcttgcggac cgaagttacg cctttttgat
      181 ggtgaaggaa acgatgtctc ctccggagat atcatcgccg agaccgcccg tatcctggac
      241 gccgggtata tcgtggcaat ccggggcatt ggcgggttcc accttgcatg tattgagagc
      301 tcagcagggg aactgaagcg ccgcctggga agatccgagc agccgtttgc ggtgatggtc
      361 aggccggacg tcgtcgaccg gatcgcaatc ctctctgccg aagaccagat gcttctcgaa
      421 agctgccagc ggcccatcgt cgtcctcggg aagcgtgacc cggatgccca ccaggatatc
      481 agtaacctcc acaccatcgg ctgcatgctc ccctataccg gcctgcacca cctgctcttc
      541 tcacgcctct cccatcccat cctgataatg acaagtgcga acatgccagg gtatccgatg
      601 attaccggca ttgatgtcgc aatggcacgg atgcacgggc acgtcgactt cttcctcacc
      661 catgaccgcc agattgtcaa ccggtgcgac gattccgtgg tcagggacgg gttcattatc
      721 cgcctctcga ggggaatggc ccccaggaga acagccattg accttgggaa tacctccata
      781 ctggcggtgg ggccggaact gaacgcaaac gtgaccatct acagggatgg gttctgcacc
      841 acatcgcccc atgtcgggaa cgtccggaac cctcagacct acgcctacct ccaggagacg
      901 atcgagaacc ttggccggct cctcggcgga aattacaagt acatcgccca tgatgcgcat
      961 ccccagttcc tctccacccg gtacgcaaga gagctcgcag gggaacatgg ggccgcactc
     1021 gtccccgtcc agcatcacca ggcccacatc gcggcgacca cgacctcgcc ctgcatcggc
     1081 atcgcaatcg acggggtcgg gtacggggag gacggcacca tctggggagg ggagatattc
     1141 gccggctcca tccccggtta cgaacgggtc ggccacctgg aagtggtccc aatgcctggc
     1201 ggagaccttg caaccaggtt tccggagagg atgctttacg gcatccttcc caccccttca
     1261 attgcggcgc tcctctcgtc ccgtggctgg accgatctcg agttatctgt ccttgactca
     1321 cagatctcca gagggatcaa cgtggtcccg acgtcgagca caggcagggt gttggacgct
     1381 gccgccgccc tcctcggcat atgcagggag aggacatacg acggagagcc cgcaatgaaa
     1441 ctcgagtcat ttgccgttgg ggggaaagcc cggcaatggg acctcgatta tatccaggtc
     1501 gggggcaagg aggtattctc cacgagagcc ctgctgcaga aggcgatggg cgagatggag
     1561 aggtgcaaag atcatgacca gagggcgatg cgttcgatcg ccgcatcgtt ccagttcaac
     1621 cttgcccgtg ggattgcagg gatggcgatc catgccgcgt acaagaggga tcttcccatt
     1681 gttgcacttt caggcggtgt ggcctataac agggtgattc gcgagaccat caggaacctg
     1741 gtgacgacat cgggcctcga gcttcgcatg aacccggatt acccgctcgg agatggatgc
     1801 atctcgtacg ggcagtgcgt gtatgctggg gtgtcagccg ggattgaaaa atagacagat
     1861 tttttttaaa atgggggttg gggattcttc aggattctca caggattggg gtgaggatga
     1921 tgagaggcgg cgtggtcgtc tttccggtct cgatggtcac gctcctgacc tgggtcttga
     1981 acccctgttt catgagtttc agttcataat cgccgggagc aagcccatgg attgttgtcg
     2041 gactggttcc tcgcgcctcg ttattgaggt atatc
//
LOCUS       DAOQVJ010000311         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_68285_length_2075_cov_4.365842, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000311 DAOQVJ010000000
VERSION     DAOQVJ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68285_length_2075_cov_4.365842"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(530..>2075)
                     /locus_tag="PKL26_06755"
     CDS             complement(530..>2075)
                     /locus_tag="PKL26_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS83429.1"
                     /translation="RSVVPGSIDPDVHTCSLAGKDGSLVNFREALLSGSLVHPGGFLL
                     QRIFPQGYIAVDTLPDQERRDTDACSAGLVWEGSLYGAIEVILPQSEWHNYSRCLDTY
                     LGVASLALQKRMFEHALRTSEERFKRIATTNPHPISIIDSSGRYIYLSPRFTELFGYT
                     QEDIPTGREWFMQAFPDLDEQKRARETWKMDLAESRPGEIRPRQFRVRCKDGLFKEIS
                     FLPVGMSNGQQLIVYEDVTPRLEAVQTRNLLFDIFRSSHDGIFSATIDGRILSWNPAA
                     ERIYGYSAEEVEGGDVCILEPPSLKGEIPAILERVRRGEHIVNFETQRIKKDGKLIDV
                     SLTVSPIYSKGRTIMGASTIIRDITARKSEERLRRAEMQYRELVDNINVGVYRSTGDP
                     EGRFVWGNTSLLRILGYDTLDDLQDIPVSDMFMHAGGRADLLAELMQHGFVKNREIIL
                     KKADGSPIHVLVTALATFAPDGSISHINGIVEDITSQRALEQKVARLSDFHDEVHLPT
                     PPPPRD"
BASE COUNT          452 a          571 c          567 g          485 t
ORIGIN      
        1 gagcagacgt gtttctcaac aatgcgcacc gcggccccac ccctatcacc ctgcactctt
       61 tggaaccggg gtcgcacaat ctcctggtga gactctccgg gtatcgggac tggcagtcgg
      121 atgtgcgggt ctctgcggga gagactgttg agataaaggc cacgttgatt caaaaccctc
      181 caagtgatag gccccaaagc ggtattcctg cacacatagc gctaggaagc cttacgattg
      241 ctgcattact gtggttcagc cggaaataat tatctccgac cggatggagc tcctggccat
      301 ggagtatatt tctgggactt tgctatctga tatgggtagg atttgtaaac tggaaacatt
      361 ttgtgggggc tgtctgcccc cacaccctta tatgcgatgc acgccgccac ccccgaagga
      421 cgccccggcg gcggttggag tgactgaagt actccacagg taacaaactc gattcaatcc
      481 ccccatacaa aagagcgagg aggcatatct ctccctgtcc cccattgctt caatcgcggg
      541 gtggaggagg ggtgggcagg tgcacctcgt cgtgaaagtc cgataatctg gctactttct
      601 gctcaagggc gcgctggctg gtgatatcct ccactatccc gttgatatgg gagatggacc
      661 catcgggtgc aaatgttgcg agcgcggtga cgagaacatg gatgggagaa ccgtcagcct
      721 ttttgagtat aatttcccgg tttttcacaa atccatgctg catgagttcg gcgagcagat
      781 ccgccctccc acccgcatgc atgaacatgt cactcaccgg gatatcctgc aggtcatcga
      841 gggtatcata gcccaggatg cggagcagcg aagtattccc ccatacgaat cttccttcag
      901 ggtcgccggt gctgcggtat actcccacgt ttatgttgtc gaccagttcc ctgtattgca
      961 tctccgcacg acgcaggcgt tcttcggatt tccgggcggt gatgtcccgg attattgtag
     1021 aggcacccat gatcgtcctc cctttcgagt atatcgggga gacagtgagc gatacatcga
     1081 tcagcttccc gtcttttttg atcctctggg tctcaaaatt cacgatatgc tccccccgcc
     1141 gcacgcgctc caggatggcg gggatctcac ctttgaggga tggtggctct aagatgcaga
     1201 cgtcgccccc ttcgacctct tcggcggaat agccataaat ccgctccgca gcagggttcc
     1261 acgagaggat tctgccatcg atggtggcac tgaatatccc gtcatgtgat gatctgaaaa
     1321 tgtcaaaaag gagattcctg gtctggaccg cttccagtcg cggagtcaca tcctcgtata
     1381 cgatcagttg ctgtccattg gacatgccaa cgggcagaaa cgagatctcc ttgaagagtc
     1441 cgtccttgca tcgcaccctg aactgccgcg gtcggatctc cccgggccgg gattctgcaa
     1501 gatccatttt ccatgtttcc cttgcacgtt tctgctcatc aaggtcagga aatgcctgca
     1561 tgaaccactc cctccctgtc gggatatcct cctgtgtata gccgaagagc tcggtgaatc
     1621 tcgggcttaa atagatatag cggcctgaag aatcgatgat ggatattgga tgggggttcg
     1681 tggtggcaat tctcttgaag cgttcttcgc ttgttctcaa cgcatgctcg aacattctct
     1741 tctgcagggc gagggatgcg acgccgaggt aggtatcaag acagcgggag tagttatgcc
     1801 attcggattg cggaaggatt acctctatgg ctccatagag tgacccttcc cacaccagcc
     1861 ccgcggaaca ggcgtcagtg tcccggcgtt cttgatcagg aagagtgtcc acggcaatat
     1921 atccttgagg gaagatacgc tgcaggagaa atccccctgg gtgaacgagg gagcccgaga
     1981 gaagagcctc ccggaaattg acaaggctgc catccttccc ggcaagggaa caggtgtgaa
     2041 cgtccgggtc gatagaacca ggcacaacac tcctg
//
LOCUS       DAOQVJ010000312         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_68319_length_2075_cov_2.740099, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000312 DAOQVJ010000000
VERSION     DAOQVJ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68319_length_2075_cov_2.740099"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKL26_06760"
     CDS             complement(<1..375)
                     /locus_tag="PKL26_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83430.1"
                     /translation="MTTGRCRDQKDSYPHLGAVAPWTGMRAGLVTGSRVRVRDTPRNN
                     VTRAFSPSLPFQQRVIPMRSVRFFVLFILVCLALPAAAVTITERPDTIANGQPIFVTI
                     RDLPGNSSFSLLVQADCAVQPNS"
     gene            complement(372..1454)
                     /gene="wecB"
                     /locus_tag="PKL26_06765"
     CDS             complement(372..1454)
                     /gene="wecB"
                     /locus_tag="PKL26_06765"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNS83431.1"
                     /translation="MICLVLGTRPEIIKLSPLIRECGRLEIPYYLLHTGQHYSYEMDR
                     VFFEDLELPAPQFNLDTGSGLHGEQTGKILAGAERVFMEHRPGAVLVQGDTNTVLAGA
                     LAAAKLHVPVGHVEAGLRSFDRRMPEEINRIVADHVSDLLFAPTETAAGYLRSEGIPG
                     EKIFVTGNTVVDAVFQNLEIARKKTGVMERMGAKEGDFILVTAHRAENVDDRGRLAGI
                     LEGIGAVGEETGMQVIFPVHPRTAKMIGEFGLDTPGIRIVPPLGYLEFLLLESKAALI
                     LTDSGGIQEEACVLGVPCVTLRENTERPETLQVGSNVLAGTNPTTIRESAREMRSRDR
                     GWENPYGDGHAGERIIGILATMGLTG"
     gene            complement(1610..>2075)
                     /locus_tag="PKL26_06770"
     CDS             complement(1610..>2075)
                     /locus_tag="PKL26_06770"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNS83432.1"
                     /translation="LIPLIIRNALRHRPLPVYGDGKNVRDWLYVEDHCRAIDMVLQNG
                     RPGEVYNVGGNNEWENIAIVRLLLSILQEETGDPGINESLVQFVKDRPGHDRRYAIDS
                     TKISRELGWEPATPFPDGIKKTVRWYLDHQDWMERVISGEYQSFYERNYEHR"
BASE COUNT          341 a          633 c          688 g          413 t
ORIGIN      
        1 ggagttgggc tggacggcgc agtcggcctg cacgaggagc gagaacgagg agtttccggg
       61 gagatcgcgg atggtcacga atatcggctg gccgtttgcg atcgtgtcgg gtctctccgt
      121 gatggttacg gcagcggcag ggagggcaag gcatacgagg atgaagagca cgaaaaagcg
      181 aacggatctc atgggaataa ccctttgctg gaaggggaga gaaggggaga atgcgcgtgt
      241 tacattgttg cggggcgtat cgcggaccct cacccggcta ccggtgacca ggcctgccct
      301 catgccggtc catggcgcca cggcccccag gtggggatac gaatccttct ggtcccggca
      361 acgacccgtg gtcatcccgt cagccccatg gtggcaagga tcccgatgat ccgctcccct
      421 gcatggccgt cgccgtaggg gttttcccag ccgcggtccc gggagcgcat ctctcttgcg
      481 ctctcccgga tggtcgttgg atttgtcccc gcgagcacgt ttgatcccac ctggagggtc
      541 tctggccgct cggtgttctc ccgaagcgtg acgcagggga cgccgagcac gcacgcctcc
      601 tcctggatac cccctgaatc ggtcaggatg agggcggcct ttgattcaag gagaaggaac
      661 tcgagatacc cgaggggcgg gacgatgcgg attcccgggg tgtccagccc aaactccccg
      721 atcatcttcg cagtccgggg gtggaccggg aagatgacct gcatgccggt ctcctctccc
      781 actgccccga tgccttcgag gattcctgcc agccggcccc ggtcgtcgac gttctcggcc
      841 cggtgggctg tcacgaggat gaagtcaccc tctttggctc ccatccgctc catgacgccg
      901 gtcttcttcc gcgcaatctc caggttctgg aagactgcgt ccaccacggt gttcccggtg
      961 acgaagatct tctcgcccgg tatcccctcg ctgcgaaggt atcctgctgc ggtctctgtg
     1021 ggtgcgaaga ggaggtcgct cacgtggtcc gcgacgatcc ggttgatctc ctcgggcatc
     1081 cggcggtcga aggaccggag gccggcctcg acatggccga ccgggacgtg gagttttgct
     1141 gccgcgagcg ctcctgcaag cacggtgttc gtgtcgccct ggacgaggac cgcaccgggt
     1201 cggtgctcca taaagacgcg ctcggcgccc gcaaggatct ttcctgtctg ctccccgtga
     1261 aggcccgagc cggtgtcaag gttgaactgc ggggccggca gttccaggtc ctcaaaaaag
     1321 accctgtcca tctcgtacga gtagtgctgc ccggtatgga ggaggtagta cgggatctcc
     1381 agtcgcccgc actcccgtat gaggggggag agcttgatga tctcggggcg ggtgccgagc
     1441 acgaggcaga tcattatcat cctgagggta ggggactgaa ggataaaaag ggggaggagt
     1501 gacggctgtc ccggatggcc gatgcgggct ctcctgcccg gtgccagtca ggagttcatc
     1561 ccggggagcg tttccgtgcg gctgccaggc gggatgggac aacaccggtt caccggtgtt
     1621 cgtagttccg ctcgtagaac gactggtatt ccccggagat taccctctcc atccagtcct
     1681 ggtggtccag gtaccagcgg acggtctttt ttatcccgtc cggaaacggg gttgcaggct
     1741 cccacccaag ttcccgggag atcttggtcg agtcgatcgc ataccgccgg tcgtggccgg
     1801 gccggtcctt cacgaactgg accaggctct cgttgatccc ggggtcccct gtctcctcct
     1861 gcaggatgga gaggaggagc ctcacgatcg cgatgttctc ccactcgttg ttccccccga
     1921 cgttgtacac ctccccgggc cggccgtttt ggaggaccat gtctatcgcc cggcagtggt
     1981 cctccacgta gagccagtcc cggacgttct ttccgtcccc gtagaccggg agcgggcggt
     2041 gtcgaagggc gtttcggatt atcaggggga tgagt
//
LOCUS       DAOQVJ010000313         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_68392_length_2073_cov_3.718038, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000313 DAOQVJ010000000
VERSION     DAOQVJ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68392_length_2073_cov_3.718038"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKL26_06775"
     CDS             <1..213
                     /locus_tag="PKL26_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83433.1"
                     /translation="RLTQLDKRYRTKYGMSMVENLNTIREHGIRAFVRIERERWTCKE
                     CGGVINVHTSRCASCGKERDDHNNFN"
     gene            576..818
                     /locus_tag="PKL26_06780"
     CDS             576..818
                     /locus_tag="PKL26_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNS83434.1"
                     /translation="MFEESHEGKHIFGTVKVGERGQIVIPKKAREVFSIQPGDTLLVL
                     GDEKRGIAIVKADAFHAMAMKMLKIFDSNPEEAPED"
     gene            843..>2073
                     /locus_tag="PKL26_06785"
     CDS             843..>2073
                     /locus_tag="PKL26_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNS83435.1"
                     /translation="MKICIISGSPKGEESITLQYVRFIEQAFPEHTFPVVHAGRDIKK
                     MEQQREAWDEALAKVHDSDGVVFATPVYFMLVPGQLKRFIEMAFERDARSAFSQKYSA
                     ALTTSIHYFDHTAHSYLHGISEDFGMHHAGSYSAHMEDLKSEVEQKRLLLFFSDFLDA
                     ISEKRAITREFLPLPKVSPTYYPSTQSTVVDTNGRRVVILHDAKPGSDQEAMVRRMAA
                     LFGGQATVADIGDSLMKGGCLGCCRCAFDNTCVYQDGFRDFWERYVVPADIMIMCGTI
                     QDRYLSATWKQWNDRSFFNGHVPALKGKQVGYLVEGPLAYLPNLREVLAARAVIGGAN
                     LVGIVTSEQGNPGEIDAALAALAERSVRFADKGYFAPVMFSAIAGRKLFRDEIWAHMR
                     AIFQADDRYYRSHGYYDF"
BASE COUNT          506 a          516 c          594 g          457 t
ORIGIN      
        1 cggctcacac aactggacaa gcggtaccgg acgaagtatg gaatgagcat ggtcgagaat
       61 ctcaatacaa tcagggagca tggcatccgg gcttttgtga ggatcgagcg ggaacgctgg
      121 acctgtaagg aatgcggggg tgtgatcaat gtgcacacgt cgcggtgtgc gtcatgtgga
      181 aaggagcggg atgatcacaa taattttaat tgaggatagt tgggatatat cacgggatct
      241 acgtgtggcg ataaaccagg aaaagggatg taccgttgtc gaacatacag agaggtgatt
      301 ctccttgaca atgacactga tgatttacct ccatgtccca gatgttaaga ctgcacctgg
      361 gataaactct aatctctttg tatcttttcc tgatggtcat tttcatcccg aatggaatgg
      421 tctgccttga tgaatggccg ggataaaggg aggccgaacc tgccttcgct cgtcagggcg
      481 gaagggaaag tcctctctcg atctcccgcc aacacattta tcatattaat catacaaata
      541 cgaatatatg aaagatatga ttagtgtgaa aagtcatgtt tgaagagagc catgaaggga
      601 aacacatctt cggcaccgta aaggtaggtg agagggggca aatcgtcatc ccgaagaaag
      661 cccgggaggt cttctccatc caacctggcg atacgcttct tgtgctggga gatgagaaga
      721 ggggcattgc catcgtcaag gcggatgctt ttcacgccat ggctatgaag atgctgaaga
      781 tcttcgacag caacccagag gaggcccccg aggactagga ttggttctgg gggaagacta
      841 gtatgaagat atgcatcatc tccgggagcc ccaagggtga ggagagtatc accctgcagt
      901 atgtccggtt catagagcag gcatttccag aacatacctt cccggtcgtg catgcgggac
      961 gggacatcaa gaagatggag cagcaacggg aggcatggga cgaggcgctc gcgaaagtcc
     1021 atgattccga cggcgtggtc tttgcaacac cggtctattt catgctcgtc ccgggccagc
     1081 tgaagcggtt catcgagatg gcatttgaac gggatgcccg atcggcattt tcgcagaaat
     1141 attctgcggc actcaccacc tctatccatt acttcgatca caccgcccat tcctacctgc
     1201 atgggatctc cgaagacttc ggcatgcacc acgccgggtc ctactcggcc cacatggaag
     1261 acctgaaatc cgaagtcgaa cagaagcgtc tgttgctctt tttctccgac tttcttgatg
     1321 caatttcaga aaagagggcc atcacgcgcg aatttctccc gctaccaaag gtctcaccca
     1381 cctattatcc ctccacccaa tcgactgtcg tggataccaa tgggagacgg gtggtaatcc
     1441 tccatgatgc aaaacctggt tccgaccagg aggccatggt ccgccggatg gctgcactat
     1501 ttggcgggca ggccacggtt gccgatatcg gggattccct gatgaagggg ggctgccttg
     1561 gctgctgccg gtgtgcgttc gacaatacat gcgtatacca ggatgggttc cgcgatttct
     1621 gggagagata cgtggttccg gccgatatca tgatcatgtg cgggaccatt caggatcgat
     1681 acctgtcagc aacgtggaag cagtggaacg accggagttt cttcaacggg cacgttcctg
     1741 cactcaaggg aaagcaggtc ggatacctgg tggaagggcc gctcgcatac ctcccgaatc
     1801 tgagagaagt gcttgcagcc cgggcagtga tcggcggggc gaaccttgtc gggatagtga
     1861 ccagcgaaca gggaaacccg ggcgaaatcg acgcagccct cgccgcactt gctgagcgct
     1921 cggtcaggtt cgcagataag ggctactttg ccccggtcat gttctcagcc atcgcaggaa
     1981 ggaagctctt tcgggacgag atatgggcac atatgcgggc aatatttcag gcagacgacc
     2041 ggtactaccg gtcccatggc tactatgact tcc
//
LOCUS       DAOQVJ010000314         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_68736_length_2067_cov_4.497018, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000314 DAOQVJ010000000
VERSION     DAOQVJ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68736_length_2067_cov_4.497018"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKL26_06790"
     CDS             complement(<1..1149)
                     /locus_tag="PKL26_06790"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNS83436.1"
                     /translation="MVMTPSSPSPQRPLDKESIRLVAKAEKVDPTVLARHVGGGSVVI
                     MRRGDRCVGIGRGLRIKVNVNIGTSNVRSVPHDEVKKAVVAEHFGADTISDLSVGRDV
                     EETRRLIFSHTSVPITTVPVYQAAAEQGIEGMDARDILDTVRRQAREGVSSMVVHCIT
                     RDILEACRREGRVLGLVSKGGSITAAFMLHHGCQNPFIEHFQEILGIMRSCGIVLSLG
                     NSVRSGCIGDGWDQAQDEELHTNLALAEEAHRAGVQVIIEGAGGHIRYDRIASTIRRY
                     RDASRFPLFVAGPLPTDIALGLDHIAGCVGASAAGAAGADYLCCITPAEHLGLPTPAQ
                     VREGLLAWRIAAHIGDLARLGLDMQDRKMAEKRALMDRKGQVLLALDPE"
     gene            complement(1256..1618)
                     /gene="nrdD"
                     /locus_tag="PKL26_06795"
     CDS             complement(1256..1618)
                     /gene="nrdD"
                     /locus_tag="PKL26_06795"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNS83437.1"
                     /translation="MQWSKEQLEMARSVRRLEDIPVPERKYKCHTCHLIVDETPCPNC
                     GDTHLQIMCPLDHCHCSHEIISTIEYCPLCGAPVCPECGSHDVVQISRVTGYLQEVSG
                     WNAGKQQELKDRVRYTLA"
     gene            complement(1728..>2067)
                     /locus_tag="PKL26_06800"
     CDS             complement(1728..>2067)
                     /locus_tag="PKL26_06800"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNS83438.1"
                     /translation="AARAMGGRCMVVAEMSHPGAVEYFSGGVAERIARMAAECGADGI
                     IAPATRPDRVRVLREIVRDRLIWSPGVGAQGGDPRAIAGLVDAVIVGRQIYGAEDPAR
                     EAAAFEFLRR"
BASE COUNT          370 a          680 c          620 g          397 t
ORIGIN      
        1 ctccgggtca agtgccagca agacctgccc cttcctgtcc atcagcgccc tcttctctgc
       61 catctttctg tcctgcatgt caaggccaag cctcgcgagg tccccgatat gcgcggcaat
      121 tctccacgca aggagaccct cccgtacctg tgcaggggtg gggagcccga ggtgctcggc
      181 gggggtgata cagcagaggt aatcggcccc cgccgctcct gccgcactgg cgccgacgca
      241 tccggcgatg tggtcgagcc cgagtgcgat gtcggtagga agcggcccgg ccacgaaaag
      301 ggggaaacgg gacgcatccc tgtaacgccg gatcgtagaa gcaatccggt cataccggat
      361 gtgccccccc gccccctcga tgatgacctg gaccccggcg cggtgagcct cctcggcaag
      421 tgcgaggttt gtgtgaagct cttcatcttg cgcctggtcc cacccgtcac ctatgcaccc
      481 gctccgtacc gagttcccga gggagagcac tatcccgcag gaccgcatga tcccgaggat
      541 ctcctggaag tgctcgatga acgggttctg gcagccgtgg tggagcatga acgccgcagt
      601 gatggatccg cccttcgaca cgagcccaag cacacgtccc tccctcctgc acgcctccag
      661 gatgtccctc gtgatgcagt ggacaaccat ggaactcacc ccttcccggg cctgtctgcg
      721 gaccgtgtca aggatgtccc gtgcgtccat gccctcgatc ccctgctcgg ccgcggcctg
      781 gtaaaccggg acggtggtga tgggaaccga agtgtgcgaa aagatgagtc gccgggtctc
      841 ttccacgtcc ctgcccaccg agaggtccga gatggtgtcg gcaccgaagt gctcggcgac
      901 aaccgccttt ttcacctcgt catgagggac tgaacggacg ttcgaggtgc cgatgttcac
      961 gttcaccttg atgcgaaggc ccctgccgat gccgacgcac cggtcccccc tccgcatgat
     1021 gaccacgctc ccgcctccaa cgtgacgggc gagcactgtg ggatcgactt tctcggcttt
     1081 ggctaccagg cggatggatt ctttgtccag gggccgttgt ggagaaggag aggagggcgt
     1141 cattaccatc ggaaaaccac ctcccaccgg ccggtgcagg agggtgaatc tttccaggaa
     1201 aattcgtcac ccggagtaga aaacaggaag gccatccagg ggaagacgcg gcaattcacg
     1261 ccagggtgta gcggacccgg tccttgagct cctgctgctt tccggcattc cagccggaga
     1321 cctcctggag gtaccccgtg accctgctga tctggacaac gtcatggctc ccgcattcgg
     1381 ggcagacagg ggcgccgcag agagggcagt actctatcgt ggagattatc tcatgggaac
     1441 agtggcaatg gtccagggga cacatgatct ggaggtgcgt atccccgcag ttcgggcacg
     1501 gggtctcgtc cacgatcagg tggcaggtgt ggcacttgta cttccgctcc gggaccggga
     1561 tatcctcgag gcggcgcacg gatctggcca tttcaagctg ttctttactc cattgcatac
     1621 attaccatgg atatttttcc atttaaacct ttagcttgtg ccagatttcg cctcagtcca
     1681 ctcatagggg tcatcaccgg gaaattccct cagagagagt tggatcatca tcggcggagg
     1741 aactcgaagg ccgccgcttc ccttgccggg tcttctgcgc cgtagatctg cctccccacg
     1801 atcactgcgt ccacgagccc tgcaatggcg cgggggtctc cgccctgtgc gcctaccccc
     1861 ggggaccaga tcagccggtc cctgacgatc tcccggagca cccgcacacg gtcagggcgt
     1921 gtggcggggg cgatgatccc gtcggcgccg cattccgctg ccattcgggc gatccgctcg
     1981 gcaactcctc ctgagaaata ctcgaccgcc cctgggtggc tcatctctgc gaccaccatg
     2041 caccggcccc ccatggcccg ggctgcg
//
LOCUS       DAOQVJ010000315         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69074_length_2061_cov_3.351944, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000315 DAOQVJ010000000
VERSION     DAOQVJ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69074_length_2061_cov_3.351944"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKL26_06805"
     CDS             complement(<1..327)
                     /locus_tag="PKL26_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HNS83439.1"
                     /translation="MLDIFKCEKCGKIVMVVSDGDGQLVCCGMPMKRQDENTVDASNE
                     KHVPVVEPHGKEILVRVGSVPHPMEPAHHLQWIEVMSGPMLYVKGLKPGEKPEAVFPV
                     STTDVKV"
     gene            454..1647
                     /locus_tag="PKL26_06810"
     CDS             454..1647
                     /locus_tag="PKL26_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNS83440.1"
                     /translation="MHDHVPARELQDRMSRFLARMDDDSQGWEMVAIFGKINLYYFTG
                     TIQDGVLLVGRDKDAVFWVRRSYDRAIEESNFPDIRPMRSYRDAALDTVSHPNRIYLE
                     TEVVPLALAERFRKHFPFSEVSPMDLQAARVRSIKSPYELALLEKAGEIHRIVLEEEA
                     PKVLRQGMSEAEFGTAIYSLMVERGHQGVVRFGRFNTEIEVGQIGFGESSLYPTCFDG
                     PGGCYGAYPGAPVLGSHTRKLRSGDLVFIDNSCGVEGYQTDKTMNYMFGRPVPEYAIE
                     IHSRCVELQDEIASLLKPGVAPSTIYTTIMAGLDQEFLTNFMGFGERTVQFLGHGVGL
                     VVDEIPVIAMGFDEPLQERMVIAVEPKKGIPGVGVVGTENTYVVTPDGGRSITGKCRG
                     LIPVY"
     gene            1773..>2061
                     /locus_tag="PKL26_06815"
     CDS             1773..>2061
                     /locus_tag="PKL26_06815"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HNS83441.1"
                     /translation="MKQNTFFLMAGLIGLIEVGIFWLSVLLKESMLITIAFVVGILVI
                     YIARRTISDKRDDERSALITQKAGARTLEVFWILFFAVSLGSLVMGFATPLG"
BASE COUNT          444 a          524 c          611 g          482 t
ORIGIN      
        1 taccttgaca tctgtcgtgg agacagggaa cacggcttcc ggcttttcgc ccggtttcag
       61 ccccttcacg tagagcatgg ggcccgacat tacttcgatc cactggaggt ggtgggcagg
      121 ctccatcggg tgaggaactg acccgaccct gacgaggatc tccttgccat ggggttcgac
      181 caccggcacg tgcttctcat tgctggcgtc gacagtgttt tcatcctgtc tcttcatcgg
      241 catcccgcaa cagacaagct gcccgtcgcc atcgctgacg accatgacaa tcttgccgca
      301 cttctcgcat ttgaatatgt caagcatgga tcatgatcac tcctacagga gagacttctt
      361 ccccggaagg gatttaatag ttcctgaccg ggatgtgcga atgccgaaaa aaagtaaggg
      421 gtaaataaca ccatggtccc attcctctgt tgcatgcatg accacgtacc cgccagggaa
      481 cttcaggacc gcatgagccg gttccttgcc cggatggacg atgacagcca gggttgggag
      541 atggtggcga tcttcgggaa gatcaacctg tactatttta cagggaccat ccaggacggc
      601 gtgctcctcg tcggcaggga caaagacgcg gtcttctggg tgcgccggag ctacgaccgt
      661 gcgattgagg agtcaaactt ccctgatatc aggccaatga ggagttatcg cgacgcggct
      721 ctcgataccg taagccatcc gaaccggata tacctggaga ccgaggttgt gccgctcgcg
      781 cttgcagaac ggttcaggaa acactttccc ttctccgagg tttcgccaat ggatttgcag
      841 gcagcccgtg tcaggtcaat caagagcccg tatgagcttg cactccttga gaaggctggg
      901 gagattcaca ggattgtgct cgaagaggaa gcgccgaagg ttctcaggca ggggatgagc
      961 gaggcggagt tcgggaccgc tatatactcc ctgatggttg agcggggaca ccagggggtg
     1021 gtccgtttcg gacgttttaa caccgagatc gaggtggggc agattggatt tggagagagt
     1081 tccctctacc cgacctgctt cgacgggcct ggcgggtgtt acggagcata tccgggggct
     1141 ccggtgcttg gtagccatac ccggaagctg cgatccgggg accttgtatt catcgacaat
     1201 tcatgcggcg tagagggata ccagaccgac aagacgatga actacatgtt cgggagacct
     1261 gtcccggagt atgcaattga gatccatagc cgctgcgtgg agctgcagga cgagatcgca
     1321 tcgctgctga aaccaggggt ggccccctcg acgatttaca ccacgatcat ggcgggtctt
     1381 gaccaggaat tcctcaccaa tttcatgggc tttggagagc ggacagttca gttccttggt
     1441 cacggcgtag ggcttgttgt ggatgagatc ccggtgattg caatgggttt tgatgaacct
     1501 ctccaggagc gcatggtcat cgcggttgag ccaaagaagg ggatcccggg ggtgggggtg
     1561 gtcggcaccg agaacaccta tgtcgtgaca ccggatggtg gccggagcat cacgggtaaa
     1621 tgcaggggac tcatcccggt atattgatgc ccgttccaga accggttttt taaatcccct
     1681 ctgctctcaa gcctgcgcct gcctcaatat gccgaagggg tacgaacata gtatagtttt
     1741 tattacttaa tgtaatatta tatttacata gaatgaagca gaacaccttt tttctcatgg
     1801 caggcctgat cggccttatc gaggtgggga tcttctggct gtcggttctt ctcaaagaat
     1861 cgatgttgat tacgattgcg tttgtagtgg gaatcctggt gatttacatt gcaaggagga
     1921 ccatttcaga taagagggat gacgaacgtt ccgcgctcat cacgcagaag gccggggcga
     1981 ggactctgga agtattctgg atactctttt ttgccgtcag cctgggcagt ctggtgatgg
     2041 gctttgccac ccctctcggc a
//
LOCUS       DAOQVJ010000316         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69485_length_2053_cov_4.713714, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000316 DAOQVJ010000000
VERSION     DAOQVJ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69485_length_2053_cov_4.713714"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..626
                     /gene="tmk"
                     /locus_tag="PKL26_06820"
     CDS             6..626
                     /gene="tmk"
                     /locus_tag="PKL26_06820"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNS83442.1"
                     /translation="MVLITLEGIDGSGKSTLNASLQHALLDLAPVFTREPGATWIGEQ
                     VRRAIADEVDPVTEALLFAADHAAHLDSVIRPALAAGKHVISDRYTDSRYAYQWATLE
                     GVLPDPLTWLRQVHDGWTVPPDHTLLLVLPVEVALSRKKQAGKREHFEEAPLLAKVQE
                     NYLRLAGEEPSRFVVVDAEMEEGELASFSERLIREWCGSSRSHRRR"
     gene            complement(583..>2053)
                     /locus_tag="PKL26_06825"
     CDS             complement(583..>2053)
                     /locus_tag="PKL26_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNS83443.1"
                     /translation="MFLRMENWVSQMEWDWCISRQRLFATPIPVWFCNRCNEMVVPDE
                     KDLPVDPTTSRPSHPCPRCGSTDFVGEEDVLDTWMDSSISVMNVTGWNGASMPPLFPA
                     ELRPQGHDIIRTWAFYTILRSVALTGSRPWDQILINGMVLGEDGFKMSKSRGNIISPE
                     EIIEKYGADALRQWAAAGAATGSDIMFNWNDVVAASRFQTKFWNIARFVLLQIERQPY
                     SRDAPIEALADRWLMARLSGTVREVTAAMEASQFDRALKAIREFSWDVLADNYIELVK
                     GRLYSEGSGRDGACRVLYRTLDTLCRLMAPFTPYFAEECYSYLGKGGVHACTWPEPET
                     EDPEELSNGNLLVQIVAELRRYKHEKGLALNAPMGRVAIFSSPITDDCGDAARALNAP
                     LDWHAEPPHLEKVPGDLQFNMAVIGPALRKDAGAFMKAARALPPDLQEKPPMELDIGG
                     RKVSVPEGAFSLQYRYLEAGEQVDVISVGDVIVTIHTTP"
BASE COUNT          394 a          623 c          651 g          385 t
ORIGIN      
        1 ggtacatggt cctgatcacc ctggaaggaa tagacggcag tggcaagagc accctcaatg
       61 cctcgctcca gcatgctctc ctggaccttg caccggtatt cacgagggag ccgggcgcta
      121 cctggatcgg cgagcaggtc cggagggcca tcgcagatga agtggacccg gtgaccgagg
      181 cactcctctt tgctgctgat cacgctgctc acctcgattc tgtgatcagg cctgcacttg
      241 cagccgggaa gcatgtcatc tccgaccggt acaccgacag caggtatgcc taccagtggg
      301 ccacgctcga gggggtgctc cccgaccccc tcacctggct ccggcaggtg catgacgggt
      361 ggacggtccc ccctgaccat acgctcctcc tcgtcctccc ggttgaggtg gcgctttcgc
      421 ggaaaaagca ggcgggaaag agggagcact ttgaagaagc acccctcctc gcaaaggtac
      481 aggaaaatta cctccgactg gcaggagaag agccctcaag gttcgtggtg gtggacgcgg
      541 agatggagga gggagaactc gcctcttttt ctgaacggtt gatcagggag tggtgtggat
      601 cgtcacgatc acatcgccga cgctgatcac gtccacctgc tctcccgcct cgaggtatct
      661 gtactgaagt gagaatgctc cctccggaac cgaaactttc cgccccccga tatcgagttc
      721 catggggggt ttctcctgga ggtcaggggg cagcgcccgt gctgccttca tgaatgctcc
      781 cgcatctttc cggagcgcag gaccgatgac tgccatgttg aactgcagat cgcccggaac
      841 tttttcaagg tgtggcggct cggcgtgcca gtcgagtgga gcattgagcg ccctcgccgc
      901 gtccccgcag tcatccgtga tgggagacga gaatatggca accctgccca tcggggcatt
      961 cagggcaagg cccttctcat gcttgtatcg ccgcagttcg gcaacgatct gcacgaggag
     1021 attgccgttc gaaagctcct ccgggtcctc cgtttcgggc tcgggccagg tgcaggcatg
     1081 cacgcccccc ttgccgaggt aagagtagca ctcttccgca aagtaagggg tgaatggcgc
     1141 catcaggcgg cagagggtgt cgagggtgcg gtaaagcacc ctgcaggcac cgtcccggcc
     1201 gcttccctcg gaatagagcc tccccttcac aagctcgatg tagttgtcgg caagcacatc
     1261 ccacgagaac tcgcggatgg ccttcagggc gcggtcaaac tggcttgcct ccatcgcagc
     1321 ggtcacctcc cgcaccgtgc cggagaggcg ggccatgagc cagcggtcgg caagagcctc
     1381 gataggcgcg tcgcgggaat agggctggcg ctcgatctgg aggagcacga atcgggcgat
     1441 attccagaac ttggtctgga agcgggaggc ggcaacgacg tcgttccagt tgaacatgat
     1501 gtccgagccg gtggctgcac cggcggccgc ccactggcgg agagcatcgg ccccgtactt
     1561 ctcgatgatc tcctcggggg agatgatgtt gccgcggctc ttgctcatct tgaagccgtc
     1621 ctcgccgagc accatcccgt tgatgaggat ctggtcccat ggccgcgacc cggtaagggc
     1681 cacggaacgg aggatggtgt agaacgccca cgtcctgatg atatcgtgcc cctgcgggcg
     1741 gagctctgca gggaagagcg gcggcatgga cgccccgttc cacccggtta cgttcatgac
     1801 cgagatggaa gagtccatcc aggtatcgag gacgtcctct tccccgacaa aatctgtcga
     1861 gccgcagcga gggcagggat gggaaggccg ggacgtggtg ggatctacag ggaggtcttt
     1921 ttcatcgggg accaccatct cgttgcagcg gttgcagaac cagaccggaa tcggcgttgc
     1981 gaagagtctc tgccgggaga tgcaccagtc ccattccatc tgcgagaccc agttctccat
     2041 ccgcaggaac atg
//
LOCUS       DAOQVJ010000317         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69506_length_2053_cov_2.721722, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000317 DAOQVJ010000000
VERSION     DAOQVJ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69506_length_2053_cov_2.721722"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKL26_06830"
     CDS             <1..780
                     /locus_tag="PKL26_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNS83444.1"
                     /translation="NERRTCRERDRYQAELQRKNAELQIAREIQESFLPPSLPALPGY
                     TLAAESRPAREVGGDFYDVIASPGGRVGLVIADVSDKGVPAALFMALSSTVMRASAAW
                     HQRPRDAVSDANALICREAGSGMFVTLFFCILDPSEQTLTCVNAGHNPPLLARADGSL
                     VELTMDGIALGADSGATYEEQTVALCPGDMLVLYTDGVTEAEDPSQQQFGDDRLAELA
                     RSERARTASQALAAIQAAVAAHTRTAPQYDDMTLLVLKVGS"
     gene            777..1229
                     /locus_tag="PKL26_06835"
     CDS             777..1229
                     /locus_tag="PKL26_06835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS83445.1"
                     /translation="MTGTETRSLTVPATLEHLEALTGMVTGFMREQGFPEKTVFEMDL
                     AVDEACTNVIRYAYAPSEGEMTVECTVSSTCARVCITDSGHPFDPLAVKAPDLTGGVE
                     ERPIGGLGVHLIRSLMDMVTYEYAAGKNILCMTKSREGVPSGSESDHL"
     gene            1257..>2053
                     /locus_tag="PKL26_06840"
     CDS             1257..>2053
                     /locus_tag="PKL26_06840"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HNS83446.1"
                     /translation="MASDTVVPGEWEAVLRTLRDRGSGTVYVVGAADRGKTTFCRWLT
                     AQLAATALSGFLDCDIGQSTLGPPATVGLALYRGALDSPRATFFRFVGAVTPSGHMVQ
                     ELVGALRLKERASEEGAPFLVVDSPGWVVEPAAQEFHVRMIDLLQPDLVVGFERGPEL
                     EGILVNFRRHPRIRIIECAAPPQVVERKRDWRRLYRFQRFSEYFADAVPQARFLSGLG
                     VHGRMPESFREESWSDLLIALCATEMMVVTLGLVDRLDLVSGTLTFRA"
BASE COUNT          355 a          638 c          701 g          359 t
ORIGIN      
        1 aacgagcgga ggacctgcag ggagcgggac cgctaccagg cagagctgca gcgaaagaac
       61 gccgagctcc agatcgcccg cgagatccag gagagctttc tcccgccctc tctccccgcc
      121 ctccccggat ataccctcgc cgccgagagc cggcccgcac gggaggtggg tggggacttc
      181 tacgacgtaa tcgcctcgcc gggggggaga gtgggcctgg tgatcgccga tgtctccgac
      241 aagggagttc ccgccgcgct cttcatggcc ctctcctcca cagtgatgcg tgcaagcgcc
      301 gcctggcacc agcggccacg cgacgcggtg agcgatgcga atgccctcat ctgccgcgag
      361 gcagggtcgg gcatgttcgt gacgctcttc ttctgcatcc tggacccttc ggagcagacg
      421 cttacttgcg tgaatgccgg gcacaacccc ccgctgctcg caagagcaga cgggtcgctc
      481 gtcgagctta cgatggacgg gatcgcgctt ggggcagaca gcggtgcgac ctacgaagag
      541 cagacggttg cgctgtgccc cggggacatg ctggtcctct atactgacgg agtcacggag
      601 gcagaagatc cgtcacagca gcagttcggg gacgaccggc tggctgaact ggcgaggagc
      661 gagcgggccc gcaccgccag ccaggcgctg gccgccatcc aggccgcggt ggctgcacac
      721 acccgcactg cgccgcagta cgacgatatg actctccttg tgctgaaggt gggatcgtga
      781 ccggaacaga gacccggagc ctcactgttc ctgcgacact cgagcacctc gaggcgctca
      841 ctggcatggt cacaggcttc atgagggagc agggctttcc cgagaagacc gtctttgaga
      901 tggaccttgc ggtggacgag gcctgcacca acgtgatccg gtacgcgtat gcacccagcg
      961 aaggcgagat gaccgtggag tgcacggtgt cttccacatg tgcgagggtg tgcatcaccg
     1021 acagcggtca cccgttcgac cctctcgcgg tcaaagcccc ggatctcacg gggggggtgg
     1081 aggagcgccc catcggggga ctcggggtgc acctcatccg gagcctgatg gatatggtga
     1141 cgtacgaata cgcggcggga aagaacatcc tctgcatgac aaagtcccgt gagggtgttc
     1201 cttcagggag cgagtctgat catttatgag gaaagagcac caagagggag agtgtagtgg
     1261 cgtctgatac agtggtccct ggcgaatggg aggcggtgct ccgcacgctc agggatcggg
     1321 gatcggggac agtatacgta gtcggtgccg cggacagggg gaagaccacg ttctgccgct
     1381 ggctgaccgc acagcttgcc gccacggccc tttcgggttt cctcgactgc gacatcgggc
     1441 agtccaccct cggccccccg gcgactgtcg gccttgccct ctaccgcggg gccctcgatt
     1501 ccccccgggc cacctttttc cggttcgtgg gggcagtgac cccgagcggc cacatggtcc
     1561 aggagcttgt cggggcgctt cggctcaaag agcgggctag tgaggagggt gcaccgttcc
     1621 tggtggtgga ctcgccagga tgggtggtgg agccggccgc ccaggagttc catgtccgga
     1681 tgatcgatct cctccagccg gacctcgtgg tgggattcga acgcggaccg gagctcgagg
     1741 ggatactggt gaacttccgc cgtcatccca ggatacggat catcgaatgc gccgcgcccc
     1801 ctcaggtggt ggagcggaag cgcgactgga ggcgcctcta ccgcttccag cgcttctccg
     1861 agtactttgc agatgcggtc ccgcaggccc ggttcctctc gggcctcggg gtgcatggga
     1921 ggatgcccga gtcgttcagg gaggagtcat ggagtgatct tctgattgcc ctctgcgcca
     1981 ccgagatgat ggtggtcacc ctcgggctcg tggacaggct tgacctcgtg tcgggcaccc
     2041 tgaccttcag ggc
//
LOCUS       DAOQVJ010000318         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69662_length_2050_cov_3.198496, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000318 DAOQVJ010000000
VERSION     DAOQVJ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69662_length_2050_cov_3.198496"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..868)
                     /locus_tag="PKL26_06845"
     CDS             complement(14..868)
                     /locus_tag="PKL26_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNS83447.1"
                     /translation="MAFESLRMELFGNVTLLDLIVFAISVFVALVIAKIVAIYMKKSL
                     SDRIPPAEMDFLIKVIQAAIVIIGVYLALPSFDINLADMLVVGGTMGLVIAFASQRIV
                     SNFGSGLFLIAERPMKPGDNVNIGTVSGTVEEIRVLSTIIKTHEGVYVRVPNEKIFTS
                     EITNFVANVARRFEYTIGISYQSDAEHAIRIVKTLLEDHPFVLRHPSPSVFVDTLADS
                     SVNLRVFIWAPSRVWWSVRTEMLWAIKKALENEGIEIPFPQQVVTFAPGTGLSVNPGC
                     GGEGEGHG"
     gene            complement(871..1581)
                     /locus_tag="PKL26_06850"
     CDS             complement(871..1581)
                     /locus_tag="PKL26_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HNS83448.1"
                     /translation="MFGIYQIPFQTDAPGITVSVESGLATSTYRRVSPEGTLTKRLGY
                     REGRVIINPVEPINLPTEVTGMLEFHFMPVTMRPNSRITICLKFPVEIGVFVGADDNY
                     TLLDVFSLAKPKYSLYGTPEKGVITRHVECEVYEDAPPTSPLEEGVLFLTLRNSGRDW
                     IEVTRAVFESSALRLFYDKTVAMAGEMEVFSRMVAETKVIDRPPEEGMKQALRVYPTK
                     KSLIPERPTFMMEYGVGD"
     gene            complement(1651..>2050)
                     /locus_tag="PKL26_06855"
     CDS             complement(1651..>2050)
                     /locus_tag="PKL26_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS83449.1"
                     /translation="FSPLLLVLETGSPAARKGAAIALGKVEDPAVMEPLLSALSDPDE
                     GVRSAVIEALAGLGEGVHSRVLAHIQGLSPGERDRVFQSLREATNPRFIEGFLPVLSM
                     ADEGVREVILSLVREGGRVQQGGKDPAPGA"
BASE COUNT          406 a          639 c          599 g          406 t
ORIGIN      
        1 ctcaagcacc gggtcaccca tggccctctc cttccccgcc gcacccggga ttgacggaaa
       61 gccccgtccc cggtgcgaac gtgaccacct gctgcgggaa cgggatctcg attccctcgt
      121 tctcgagcgc cttcttgatc gcccagagca tctcggtcct cactgaccac cacaccctgg
      181 aaggtgccca gatgaacacc cggaggttca cgctcgaatc cgcgagggtg tcgacgaata
      241 cgctcggtga ggggtgcctg agcacgaacg ggtgatcttc gaggagcgtt ttgacaatcc
      301 ggattgcgtg ttcggcatcg gactggtagc tgatcccgat ggtgtactcg aacctgcgcg
      361 caacgtttgc gacgaaattg gtgatctcgg aggtgaagat cttctcgttc gggaccctga
      421 cgtacacccc ctcgtgcgtc ttgatgatgg tggagagaac ccgtatctcc tcgacagtgc
      481 cgctcaccgt gccgatgttg acgttgtccc cgggcttcat ggggcgttcg gcgatgagga
      541 agagcccgga gccgaagttc gagacgatcc tctggctcgc aaacgcgatg acgagcccca
      601 ttgttccgcc caccaccagc atgtccgcga ggttgatgtc gaaactcggg agcgccaggt
      661 ataccccgat gatgacgata gccgcctgga tgaccttgat caggaagtcc atctctgcag
      721 gcgggatcct gtcgcttaag gacttcttca tgtagatggc cacgatcttt gcgatgacca
      781 gggcaacaaa gaccgagatt gcaaagacga tcaggtcaag cagggtcacg ttcccgaaca
      841 gttccatcct gagggactca aacgccattg tcagtcaccc acaccgtact ccatcatgaa
      901 cgtcggcctc tcggggatca ggctcttttt tgtcgggtat accctgagag cctgcttcat
      961 tccctcctcg ggggggcggt cgatgacctt tgtttcggcc accatccgtg aaaagacctc
     1021 catctccccg gccatcgcca ctgtcttatc gtagaaaagc cggagcgccg agctctcgaa
     1081 aacggcgcgg gtgacctcga tccagtccct gcccgagttc ctgagcgtga gaaagagcac
     1141 cccctcttca agggggctcg tggggggggc atcttcgtac acttcgcact ccacgtgcct
     1201 tgtgatgact cccttctcag gcgtgccgta gagcgagtac ttgggtttgg ccagagagaa
     1261 gacgtcgagg agcgtgtaat tgtcatccgc ccccacgaat accccgatct ccaccgggaa
     1321 cttcaggcag atagtgatcc gtgagttcgg cctcatagtg acaggcatga agtggaactc
     1381 gagcatcccc gtcacctccg tgggaaggtt gatcggctcg acagggttga tgatcaccct
     1441 cccttcgcgg tacccgagcc gcttcgtgag ggtcccttct ggagagaccc tccggtaggt
     1501 ggaagtggcc aacccgctct ccacggaaac ggtgatcccg ggtgcgtctg tctggaaagg
     1561 gatctggtat attccaaaca caggaagaga tgggactcgc accacatata tttgaccggg
     1621 acccgggaat cagcgggaag caggagggat ttatgcaccg ggtgcagggt ctttcccccc
     1681 ctgctgcacc ctccctccct cgcggaccag ggagaggata acctccctca ccccttcatc
     1741 tgccatggag aggaccggta aaaatccctc gatgaacctc gggttggtcg cctcccggag
     1801 ggactggaag acccggtccc tctctcctgg agagagtcct tggatatgag cgaggacgcg
     1861 ggagtgcacc ccctccccga ggcccgcaag ggcctcgatc accgccgagc gcaccccttc
     1921 gtcaggatcc gagagcgcgg agagaagcgg ttccatcacc gcgggatctt cgacctttcc
     1981 aagcgcaatt gcggctccct tccgggcggc cgggctcccg gtctccagca cgagcagcag
     2041 gggagagaac
//
LOCUS       DAOQVJ010000319         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69667_length_2050_cov_3.012030, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000319 DAOQVJ010000000
VERSION     DAOQVJ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69667_length_2050_cov_3.012030"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..498
                     /locus_tag="PKL26_06860"
     CDS             79..498
                     /locus_tag="PKL26_06860"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS83450.1"
                     /translation="MASRLMEYFNRQPRIGTLSTSGKDGKVDVAVFGSPHMTDGKTVI
                     MGLGKNRSLSNLMENPHAVYMIMEPGKSIMDWKGIRVYLVAKDIATSGPMLNDLKNQI
                     AKIAGEAAAGMIHAAARFEATEVRPLVDMGQGWEKSI"
     gene            complement(650..1090)
                     /locus_tag="PKL26_06865"
     CDS             complement(650..1090)
                     /locus_tag="PKL26_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83451.1"
                     /translation="MKRFVCVIMVLAIAVLACGCTTPAPSSQAATEAAAVPDLIGNWA
                     GTMKGFIEEKGYTDYPDEAMTMRVTEQRGRIFSGEIVFSNQTATHVVKAFAGAINPDG
                     RTFSIVEHGGGSSTGTLLSPGELELVYIERTVPFTIAIDTLKKS"
     gene            1301..>2050
                     /locus_tag="PKL26_06870"
     CDS             1301..>2050
                     /locus_tag="PKL26_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNS83452.1"
                     /translation="MASHIHRIEVRYRDDPRQKTRTDRIRSLGFPVDAVHLVDVYTIA
                     TRARDFSHAELSEIGAQLVNPVVQEYTVDQATNAAFDSAIEVGFLPGVTDNVGTTARQ
                     TIEDFSGYRFGEGEAVFSSQLYFVCGALSPESVRGLSTTLANPLVNRVHVKTRDEYGE
                     KGMDTVAPFVLLHDLPTAETVDLELDDKELARLGKEGIRDPLTGERRGPLALDLPELH
                     AIRDYFRSLGRRPTDVELESLAQTWSEHCKHT"
BASE COUNT          428 a          614 c          622 g          386 t
ORIGIN      
        1 cccctggcgg tacaggtgcg cccccccgca acttttaagc agcgtgactg aaaagccaga
       61 tgtgaagggg gaatgcatat ggcttcaaga ctgatggagt acttcaacag gcagccgcgg
      121 atcgggacgc tcagcacctc cggaaaagac gggaaggtcg acgtcgccgt cttcggctcg
      181 ccgcacatga ccgacgggaa gaccgtgatc atgggccttg ggaagaaccg ctcgctctca
      241 aacctcatgg agaacccgca cgcggtctac atgatcatgg agccgggaaa gagcatcatg
      301 gactggaaag ggatacgtgt ctacctggtg gcaaaggata tcgccacttc ggggcctatg
      361 ctcaacgacc tcaagaacca gatcgcgaag attgcaggag aggctgcggc agggatgatc
      421 catgccgcag cgaggtttga ggcgaccgag gtacggccgc tcgtagacat ggggcagggg
      481 tgggagaagt cgatctgaac aggtgttcct gatctcccgg agaacctgca gcgcactcag
      541 gggatggcag cgttgcaagc cgcaacggag tataccgctc catgccgcat gccggcccgg
      601 gctcccgatt acagatccct ctcttcagag agagaggggg ttctctctct caactcttct
      661 tcagcgtgtc gatcgcgata gtgaacggaa ctgtcctctc gatgtagacg agctcgagtt
      721 cgccgggtga gagaagtgtc ccggtcgatg acccaccccc atgctcgact atggagaagg
      781 tccggccatc aggattgatc gctcctgcaa atgccttgac cacgtgggtg gcggtctggt
      841 tcgagaagac gatctctccc gagaatatcc ttcctctctg ctcggtgacc cgcatggtca
      901 tcgcctcgtc cgggtagtcg gtgtatcctt tctcctcgat gaaccccttc attgtgcctg
      961 cccagttccc gatgaggtcc ggtaccgctg ctgcctctgt tgcggcctgg gaagagggcg
     1021 cgggggtcgt gcacccgcag gcaaggaccg cgattgcgag aaccatgatt acacacacga
     1081 atcgtttcat acacttcctg ttgacgtgcg aagagaaaaa cccggatact tcggtattat
     1141 aaaaaaatcc ggatcgaacc ggatctccgt ggaatgggtt ccccatcgga tatgagtggg
     1201 acgtgcaagc tgggtcaatg ggctgggccg gatgacgcag atgcacgagg ccgtgcgtgc
     1261 gtcggtagct attttgtcgg tttacgcccc aatagtatcc atggcttctc acatccaccg
     1321 gatagaagtc cgctaccggg acgacccgcg gcaaaagacc cggaccgatc ggatccgctc
     1381 ccttgggttt cccgtcgatg cagtgcacct ggtggatgtc tataccatcg ccacccgcgc
     1441 aagggacttt tcgcacgccg aactctcgga gatcggcgcc cagctggtca accccgtcgt
     1501 gcaggagtat accgtcgacc aggcgaccaa tgcggccttt gactctgcga tcgaggtcgg
     1561 attcctgccc ggcgttaccg acaacgtggg cacgaccgca cggcagacca tcgaggactt
     1621 ttccggatac cggttcggcg agggcgaggc agtcttctcc tcgcaactct actttgtctg
     1681 cggagcactc tcccccgaat ccgtcagggg gctttcgacc acgcttgcaa accccctcgt
     1741 gaaccgcgtg cacgtgaaga cacgggacga gtacggggag aagggcatgg acacagtcgc
     1801 accattcgtc ttgctgcacg atctgccgac cgccgagacc gtcgacctcg aactcgacga
     1861 caaggaactc gcgaggcttg gaaaggaggg gatccgggat cccctcacgg gggagcggcg
     1921 cgggcccctc gcgctcgacc tccccgagct ccatgcaata cgggactatt tcaggagcct
     1981 cgggcgcagg cctacggacg tcgagctcga gtcgctcgcg cagacctgga gcgagcactg
     2041 caagcacacc
//
LOCUS       DAOQVJ010000320         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69924_length_2045_cov_3.492965, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000320 DAOQVJ010000000
VERSION     DAOQVJ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69924_length_2045_cov_3.492965"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKL26_06875"
     CDS             <1..238
                     /locus_tag="PKL26_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNS83453.1"
                     /translation="QEMSFNVRQGMTRADDRMPQRPEVAGTSKGKEDLARHQVLLDAY
                     YSLHGCTPLTGIPNRTELERLGLRDVADSLHMEN"
     gene            384..917
                     /locus_tag="PKL26_06880"
     CDS             384..917
                     /locus_tag="PKL26_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83454.1"
                     /translation="MADEPHSGGGKDERPPAPPTEKKIPRFSLIAGAVIVILVILIIA
                     AFLTLSVQADLPPEPGTVYPYTTTYAVLIPDGLPIRIAGTPILVLTSGDELIMKIGDQ
                     NEKFVAGQTKTISERRAEFRALGIPLLSTNYLIEATYRGRSGPHADFFLIVRTSRQVP
                     SFLIERILPAEIQARPA"
     gene            1139..1882
                     /locus_tag="PKL26_06885"
     CDS             1139..1882
                     /locus_tag="PKL26_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNS83455.1"
                     /translation="MLVTFLGTNGWYDTLTGNTCSVLVQSKDYNVILDAGNGIAKADQ
                     YIDQELPTFLLISHCHLDHIAGLHTLNKFRFSLPLHICGQKGISRELHSIVREPFTVP
                     FDHLPFPVRFIELVEGTHEIPFPIECRQLVHTAPCVGYALTLDGKKIAYCTDTGFCAN
                     AVLLARDADLLITECGLKPGETSPEWPHLNPGDAIRIAREARAKRLALVHFAAHTYTT
                     LEERFDIERMYEEECPGLVAATDGLAIEV"
BASE COUNT          453 a          601 c          523 g          468 t
ORIGIN      
        1 ccaggagatg tcgttcaacg tgcggcaggg gatgacccgc gcagatgaca ggatgcctca
       61 gcgcccggag gttgccggga ccagcaaggg aaaagaagac ctggcacggc accaggtact
      121 cctggatgcc tattactccc tccatggctg cacccctctg accggcatcc cgaaccggac
      181 cgaactcgaa cgcctcggcc ttcgggacgt ggcggattcg ctgcacatgg agaattgaag
      241 gtagtgcgat cgtctttccc tgttccgcgg gcaatctgag agccaggcaa ccaagaatga
      301 cgcccccggc atcttccctg atgaagcatg ctggattacg caagatataa gtgagataat
      361 ctggactcta cctgatgagt acaatggccg atgaaccaca ttcaggggga gggaaggatg
      421 agaggcctcc tgctccccca actgaaaaga agattcccag gttctccctg atcgcgggag
      481 cggtaatcgt catcctcgtc atcctgatca tcgccgcatt tcttactctt tccgtccagg
      541 cagacctccc gcctgaacct ggcaccgtct atccctatac cacgacgtat gccgtcctga
      601 ttcctgatgg gctgccgatc agaattgcgg ggactccgat ccttgttctt acttcgggcg
      661 acgagttgat catgaagatt ggggaccaga acgagaagtt cgttgccggg cagacaaaga
      721 ccatctccga acggagggca gagttcaggg cgctcggaat ccccctcctc tccacgaact
      781 acctgataga ggcgacgtac cggggccgtt caggcccgca tgcggacttc ttcctgatcg
      841 tccgcacctc gcgccaggtc ccctccttcc tcatcgaacg gattcttccc gccgagatcc
      901 aggcgaggcc ggcctgatct tacccataaa tatcattttt ccggcggatt ggttcgctcc
      961 gggccaggga tgtcctgcga tgctccctgc attacgcaca tcctgttccc ttcatccggg
     1021 ccttggacaa gacgggaccc catttccttt ctcgacataa tatcccgttc attcccaggg
     1081 ttttttcaaa agttgagagt gcccggtttt tcaccttccg cgccaaccac tctggagaat
     1141 gttggttaca ttccttggga caaacgggtg gtatgacacc ctcaccggga atacctgctc
     1201 ggtgctggtc cagtcaaaag actataacgt gatcctagat gctggcaacg gcatcgcaaa
     1261 ggctgaccag tatatcgacc aggaattgcc cacgttcctt cttatcagcc attgccacct
     1321 cgaccatatc gccgggctgc acactctcaa taaattcagg ttcagtcttc ctctgcacat
     1381 ctgcggccag aaaggtatct cccgggagct tcactcgatt gtgcgggaac cgttcaccgt
     1441 accgttcgat catttgcctt ttcctgtcag gttcatcgag cttgtcgaag ggacccatga
     1501 gatcccattt ccgatcgagt gcaggcagct tgtccacact gccccgtgtg ttgggtacgc
     1561 acttactctc gatggaaaga agatcgcgta ttgcacagac acgggcttct gcgcgaatgc
     1621 cgtcttactt gcacgcgatg cggacctcct catcaccgaa tgcggactta aaccaggaga
     1681 aacgagcccg gaatggcccc acctgaaccc cggggatgcc atccggattg cacgggaagc
     1741 acgtgcaaaa cggcttgccc tggttcattt tgccgcccac acctacacca cccttgaaga
     1801 gaggttcgat attgagagga tgtacgaaga agagtgcccg ggcctcgttg ctgcaactga
     1861 tggcctggca atcgaggtgt gaaatcgatc gttcgcattt gaagcaagat ttcaggtatg
     1921 tatcacgggt tcatggatat tcaaggtaaa tcgcaggtat aactgttaat cgggaacaca
     1981 catgactgac acggtgaatt cgcgattttt ttacgaactc aacattttct ctctgattat
     2041 cctca
//
LOCUS       DAOQVJ010000321         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69926_length_2045_cov_3.378894, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000321 DAOQVJ010000000
VERSION     DAOQVJ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69926_length_2045_cov_3.378894"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKL26_06890"
     CDS             <1..668
                     /locus_tag="PKL26_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83456.1"
                     /translation="LVSGSHDRSAIVWDLESCEMNARISGHSHVVSCLGISGDGRILA
                     TGSWDRAIRLWSLPSGAPMGTPLIHAGRVRSLAVHPDGNLLVSAVDGGTISIWALPGG
                     ELIRNRVVRADARTGICLIPGMHWGASAGLDGSLNLSGLPWAKPMSRTTPADIGYVQT
                     CISRASSHQDIRQWKFLERLLAGRFRQCIAYGGAGMPAGPHDIEIVEEPVRSEAVACG
                     VRM"
     gene            668..>2045
                     /locus_tag="PKL26_06895"
     CDS             668..>2045
                     /locus_tag="PKL26_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNS83457.1"
                     /translation="MPLRVSIDFGTSNTVIACWDEGQGSARVREIPGWSRPCNAEPRS
                     TPVIPSLIHYGGEEVRWIGQQVHDRGLVDSPSTVRWMKHYISTRSPVRVPLPGGSRSY
                     REVGADFLQAILSSILPAQGCEEIEAVFPVPVESFEHYQDWIISLAGSAGIGRVRVVD
                     EATAAAVASGFTLIPGEVFMLFDMGGGTLDVSVVRLEDKGDSPGRRCRVLGKAGDDIG
                     GMRMDQWIFGEVWERCMSTCDERSRKALSRPLLSACESAKEALSLGEETEISACTGTH
                     DRCELSIPYTRTEFERLMEEKGVFPRLNAVIQRALQGAYSRGFAEDDLAGVVMVGGCS
                     QIPAIKKALEYRFGRQNVRHAHPLETVARGAAACAAEIDLDDHVQHDYALRFWNPRSA
                     EHEYRILVRQGDPYPSIRPVARLLVRATYDGQSQLGVPIFEMGKARGNARSSQRELVS
                     EPGGGVRLM"
BASE COUNT          362 a          623 c          677 g          383 t
ORIGIN      
        1 tcctcgtcag cggttcacac gaccggtctg ccatcgtgtg ggatctggaa tcgtgcgaaa
       61 tgaatgcacg catttccggc cactcgcacg tggtctcctg ccttgggatt tcgggcgatg
      121 gccgtatcct cgcaaccggg agctgggacc gggcaatccg gctctggagc ctcccatcag
      181 gtgcgccgat gggaactccc ctcatacatg ccgggagggt ccggtccctc gcggtgcacc
      241 ctgacgggaa tttgctcgtg agcgccgtcg atggcgggac catctccatc tgggcactcc
      301 ccggagggga gctcatccgc aaccgcgtgg tcagggccga tgcgcgcacc ggcatctgtc
      361 tcatccccgg tatgcactgg ggggcttccg caggcctgga cggatcactt aacctctccg
      421 ggctcccctg ggcaaaaccg atgagccgga ccacccccgc cgatattgga tacgtgcaga
      481 catgcatcag ccgcgcttcc tctcatcagg atatccggca gtggaaattc ctcgagcgcc
      541 tcctggccgg caggttccgc cagtgcatcg cgtatggcgg tgccgggatg ccggccggcc
      601 cgcatgatat tgagattgtc gaagaaccag tgagatccga agccgtcgcg tgcggggtga
      661 ggatgtaatg ccactcaggg tctccatcga ttttggcact tcgaacaccg tgattgcatg
      721 ctgggacgaa ggccagggat ccgcacgggt gcgggagatt cccgggtggt cccgcccctg
      781 caatgcagaa ccgcgaagca ccccggtgat cccctccctt atccactacg gcggggaaga
      841 ggtccggtgg atcggacagc aggtgcatga ccgcggcctc gtcgattccc cttccaccgt
      901 tcgctggatg aagcactaca tctccaccag gagcccggtc cgggtcccgc tgcccggagg
      961 gagcaggagt taccgcgagg tgggtgccga tttcctccag gccatactct catccatcct
     1021 gcctgcacag ggttgtgagg agatcgaggc tgtcttcccg gtgccggtag aatcgttcga
     1081 gcattaccag gactggatca tctcccttgc cggctccgcg ggaattggca gggtccgggt
     1141 agtcgacgag gcgactgccg ccgcggtggc gtccggcttc acgctcatac ccggggaggt
     1201 gttcatgctc tttgacatgg ggggcgggac ccttgacgtc tcggtggtcc gcctcgagga
     1261 caaaggtgat agtcccggcc gccgctgcag agtgctcggg aaagcggggg atgatatcgg
     1321 cgggatgcgg atggaccagt ggatcttcgg cgaggtatgg gagaggtgca tgtccacctg
     1381 cgatgagcgg tcgagaaagg cgctctcgcg tcccctgctc tcggcatgcg agagtgcaaa
     1441 ggaggcacta tcccttggag aggagacgga aatttccgcg tgcaccggca ctcatgaccg
     1501 gtgtgaactc tcgattccct acacgaggac cgagttcgag cgcctcatgg aagagaaggg
     1561 ggtctttccc cgcctcaatg cagttatcca gagggcactc cagggggcct attcgcgggg
     1621 ttttgcagag gacgaccttg ccggcgtggt catggtgggg gggtgcagcc agatcccggc
     1681 cataaaaaaa gcgctcgagt accggttcgg gcggcagaat gtccggcacg cccatcccct
     1741 ggagacggtt gcccggggag cggcagcgtg cgcggccgaa attgaccttg acgaccatgt
     1801 ccagcacgac tatgccctca ggttctggaa cccgcggagc gcagagcatg aataccgtat
     1861 cctcgtccgg cagggtgacc cgtacccgag cattcgcccc gtggcccgtc tcctcgtcag
     1921 ggcaacctat gatggccagt cccagttggg agttcccatc ttcgagatgg gaaaagccag
     1981 gggtaatgcc cggtcttcgc agagggaact cgtgagcgag ccggggggcg gggtccggct
     2041 catgg
//
LOCUS       DAOQVJ010000322         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_69979_length_2044_cov_3.364002, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000322 DAOQVJ010000000
VERSION     DAOQVJ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69979_length_2044_cov_3.364002"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PKL26_06900"
     CDS             complement(<1..577)
                     /locus_tag="PKL26_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS83458.1"
                     /translation="MIKLEDVSYCYPQGGKEALSGVSLSVRPGECIFVTGPSGAGKTT
                     LCMAAAGILEHEYGGKKGGRVTIRGRDVKEYPSLSDISGLVGMVFDDPEAQLIFTTVE
                     EEILSALERRGLSPAEIEDRLSSIMEITHLSLLKERAPHALSGGQKQRVVLATTLALG
                     TDILILDEPTAELDEQGTESIVSVLKDLKAEG"
     gene            complement(578..1096)
                     /locus_tag="PKL26_06905"
     CDS             complement(578..1096)
                     /locus_tag="PKL26_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83459.1"
                     /translation="MKSKDIAIVGILLAIGAILRYFLAMLHTPLTPNMIIAFYCLAII
                     LIRPKVLEALGIGIVAGILSMLISSSIFPPANLISEPIGALVCFGLYAVLKDRIGGPA
                     VATFTTTLASGFSFAAIALLAVAPKILDKYSTVFGFILVFVPIVVITAVFNAIIVQIL
                     YYPANRVLNRGS"
     gene            1391..>2044
                     /locus_tag="PKL26_06910"
     CDS             1391..>2044
                     /locus_tag="PKL26_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83460.1"
                     /translation="MPTMRVRYPVLLAITFLLLCGIAIAHSPSDMRLDYDHGLGTLLV
                     SVTHQVDDSSTHYVREVVIQKNGVTVDTVTYASQPSRETFTYSYPLTLEPGDVARVTA
                     RCNIGGSITREYTRPAAQGQPATVIPTTPSAAPSLWVVHAALLLAAILLLLGVAILPT
                     YGRGIAGWYRLHAGLATVGGILAIVAIAMVFGTIPSGTIRDAWNAHVALGLIVLILLL
                     "
BASE COUNT          423 a          589 c          588 g          444 t
ORIGIN      
        1 ttccctccgc cttgagatct tttaggactg agacaatgct ttccgttccc tgctcgtcga
       61 gttccgcggt gggctcatcg aggatgagga tgtcggtgcc aagcgcgagc gtcgttgcca
      121 gcacaacccg ctgcttctgc cctcccgaga gggcatgagg ggctctctct ttcagcagag
      181 agaggtgggt gatctccatg atggacgaaa gcctgtcttc gatctctgcg ggcgagagcc
      241 ccctccgctc gagcgctgaa aggatctcct cttccacggt ggtgaagatc agctgggcct
      301 cggggtcgtc gaagaccatg cccaccagtc cggagatatc ggagaggctt gggtactctt
      361 tgacgtccct tccccggatg gtgacccggc cgcccttctt cccgccatat tcatgctcga
      421 gtatgcctgc tgcagccatg cagagggtgg tctttcccgc cccagatgga ccggtgacga
      481 agatacactc tcctggcctg acagagagcg agacgcccga gagcgcctct tttcccccct
      541 gcgggtagca gtagctgaca tcctcgagct ttatcatcta cgacccccgg ttcagcaccc
      601 tgttcgcagg gtaatacagg atctggacga tgatggcgtt gaacaccgcg gtgatcacga
      661 caatcggcac aaagaccagg atgaacccaa agaccgtcga gtacttgtcg aggatctttg
      721 gagcgactgc gagcagggcg attgcggcaa aggagaaacc gcttgcaagg gtggtcgtga
      781 acgtggcgac cgcgggtccg ccgatacggt ctttcagcac ggcgtacagc ccgaagcaga
      841 ccagtgcccc gatcggttcg ctgatgaggt ttgccggggg gaagatggag ctcgagatga
      901 gcatcgagag gatccctgcg acgatgccga tgccgagggc ttcgagcacc ttcggcctga
      961 taaggatgat cgccaggcag tagaaggcga tgatcatgtt cggggtcagc ggggtatgca
     1021 gcatggccag gaagtagcgc aggatggcgc cgatggccag caatatgcca acaatggcaa
     1081 tgtcttttga tttcatgtgt gcatcacgtc tggtaggaga ttggtattcg aagggcagat
     1141 cagatggctc taacgagcaa tgatatggga ctttcaaaag gaagaacgcc agggagccag
     1201 caccggaaag tacacagaag atttctgcat ctttcgcgga gttgcgtagg caatatcaat
     1261 gcagggcatt tccgtctcta cacctatgta ctttgatgta taaaattata cattcatgta
     1321 taaaaaaatg ttgaaaaatg agctacttct gggggagaac ccttattact cctgtcacag
     1381 agggtagtgc atgcccacaa tgagagtcag gtatccagtc cttctcgcta tcaccttcct
     1441 tctcctctgc ggcatcgcca ttgcacactc cccttctgac atgcgcctcg attacgacca
     1501 cggcctgggc acccttctcg tgtccgtgac ccaccaggtc gacgattcct ccacccacta
     1561 tgtcagggag gtggtgatcc agaagaacgg agtaacggtt gacacggtca cctatgcgag
     1621 ccagccgtcg agagagacct tcacgtactc atatccgttg acccttgaac ccggggacgt
     1681 ggcacgcgtc acggccaggt gcaacatcgg gggttcaatc acccgcgagt atacaaggcc
     1741 cgcagcccag ggccagcctg ccaccgtgat ccccaccacg ccctcggcgg ctccctccct
     1801 ctgggtcgtg catgccgcgt tgcttctcgc tgcaatcctg ctcctcctcg gtgttgccat
     1861 cctccctacg tatggaagag ggattgcagg ttggtatcgc cttcacgctg gcctggcgac
     1921 ggtaggaggg atccttgcaa tcgtggcgat tgcgatggtc tttgggacaa tcccttcggg
     1981 aaccatccgc gatgcctgga atgcccatgt ggcgctcggg ctcatcgttc tgatcctcct
     2041 cctc
//
LOCUS       DAOQVJ010000323         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_70125_length_2041_cov_5.171702, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000323 DAOQVJ010000000
VERSION     DAOQVJ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70125_length_2041_cov_5.171702"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKL26_06915"
     CDS             <1..492
                     /locus_tag="PKL26_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNS83461.1"
                     /translation="RPKEIRTSGCVGVIQDEHTMRVPLPIAARVSRSVIYCAMECLND
                     HAVLWRETGGTHCTVLYDTTGTAVSHAEDVGRHNSVDKAIGKALLSGIDPGKTFLCCS
                     GRMPEGMVSKVYHAKIPIVVTNNAPSAAGIDLARRVKLTLAGFVRPPRMNIYSVPERI
                     LLG"
     gene            complement(504..1337)
                     /gene="tsaA"
                     /locus_tag="PKL26_06920"
     CDS             complement(504..1337)
                     /gene="tsaA"
                     /locus_tag="PKL26_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNS83462.1"
                     /translation="MDNSLVLTPVGVVHSPVHESRIMPVQGVRGQIEIFPEFVPALDG
                     VETSSHLILLAWMHRGERDLLTARARKISEDLPLKGVFSLRSPSRPNPLSVSVVRLLC
                     RKEGGILEVDSLDCIEGTPVIDIKPYQPGWDCVFSATHHDRSVKIEKMGPSEYRQMLI
                     REAVNYHGEWCPGAAIAVRVAECATRALGGDLTRPEVHLFCGGHPCITDSLIGITAAR
                     RGNGRLHPVAREENRGLECCEFSIKRYRVVFSISRLPEDIPYILSCRNEDLFSTRVFT
                     T"
     gene            complement(1403..2041)
                     /locus_tag="PKL26_06925"
     CDS             complement(1403..2041)
                     /locus_tag="PKL26_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS83463.1"
                     /translation="MPTIPEYTPGELLAKMESMGIPESLRESFRKCIDFHTYAAPGML
                     IGVFMVDCALKILGRRPEEKIFVTCETHKCLPDPPQVIMHATTGNHQLRVLPIGKFAM
                     TFTPSSVKEYAEGIRIYVDRKKLDQYPALASWYDNSAAFKPATMKRQLAEEILAAACG
                     MFSHERVRMKVTHKKKWNSVTCPSCGEQVPQDLMEGTTCAGCGSLAYYEKVT"
BASE COUNT          433 a          555 c          593 g          460 t
ORIGIN      
        1 cggccaaagg agatccggac ctccggctgt gtgggggtca tccaggacga gcacactatg
       61 agggtgcccc ttccaatcgc tgcgcgggtg tccaggagtg ttatttattg cgcaatggaa
      121 tgcctcaacg atcacgcggt gctctggcgg gagacgggag gtacccactg caccgtcctc
      181 tatgatacca ccggcacggc ggtatcccat gcagaggacg tgggaaggca caattccgtt
      241 gacaaggcga taggaaaagc gctgctgtcg gggattgatc ctggaaagac gtttctctgc
      301 tgttcgggaa ggatgcccga agggatggtg agcaaagtct accatgcgaa aatcccaatc
      361 gtcgtcacca ataatgcccc ttcggcagca ggcatcgacc tggcgaggag agtgaagttg
      421 acccttgccg ggttcgtccg cccccccagg atgaatatct acagtgtccc ggagcggatt
      481 ctcctgggct gatggatctt gaatcaggtc gtgaatactc tggtggagaa gagatcttca
      541 tttctgcagg agaggatgta cgggatatcc tccggcagtc gtgatatgga aaaaacgact
      601 ctgtatctct ttatagaaaa ttcacagcat tccaggcccc ggttttcctc ccgggcgacg
      661 gggtgcaacc gaccgtttcc cctccttgcc gcggtgatcc cgataagcga gtccgtgatg
      721 cacgggtgac ccccgcaaaa gagatgcact tcgggacggg tgagatcccc gccaagggcg
      781 cgggttgcac actctgcaac ccgcaccgca attgcggccc ctgggcacca ttcgccgtgg
      841 taattcaccg cttcgcggat gagcatctgg cggtattcag atgggcccat cttctctatc
      901 ttcaccgacc ggtcgtggtg ggtggccgaa aatacgcagt cccatcccgg ctggtagggc
      961 ttgatgtcga tcacgggagt gccctcgatg cagtccaggg agtcgacttc gagtattccg
     1021 ccctcctttc tgcacaggag tcttaccacg ctcacagaga gagggttcgg ccgggaaggg
     1081 gatcggaggg agaatacgcc tttgagaggg agatcttctg atattttccg tgctctcgcc
     1141 gtgagcaggt cacgctctcc gcgatgcatc caggcaagaa gtatcaggtg cgagctggtt
     1201 tccacgccat caagagcagg gacaaactcc ggaaaaattt caatctgtcc tctcacaccc
     1261 tggacaggca ttattcggct ttcatgcacg ggagaatgga ccacacctac gggtgtgagc
     1321 accagcgaat tgtccatatc ccgtttccct ttcccatgca ccctatgcgt tcccctgtgt
     1381 gaagtggatt gccgtggtga agtcaggtta ctttctcata ataagccagg ctgccacaac
     1441 ccgcacaggt ggtgccctcc ataaggtctt gtgggacctg ttcgccgcag gaagggcatg
     1501 tgacggaatt ccactttttc ttgtgggtga ccttcattcg taccctctca tgggagaaca
     1561 tcccgcaggc ggcggccagg atctcttcgg caagctgccg cttcatggtt gcaggtttga
     1621 acgcagcgct gttgtcatac caggaagcca aagcagggta ctggtcaagc ttcttcctgt
     1681 ccacgtagat gcggataccc tcagcatact cttttaccga agagggagtg aaagtcatgg
     1741 caaatttccc gataggaagg acccggagct ggtggttccc agtcgtggca tgcatgatga
     1801 cctgcggcgg gtccgggagg cacttgtgcg tttcgcaggt aacaaatatc ttttcttcag
     1861 gtcttctgcc gagaatcttg agagcgcaat cgaccatgaa gaccccgatc agcatgccgg
     1921 gggcggcata tgtgtggaag tctatgcatt tcctgaatga ttcgcgaaga gactcgggga
     1981 tccccatcga ttccatcttc gcgagcagtt cgcctggagt atattccggt atggtaggca
     2041 t
//
LOCUS       DAOQVJ010000324         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_70716_length_2030_cov_4.494684, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000324 DAOQVJ010000000
VERSION     DAOQVJ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70716_length_2030_cov_4.494684"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1847
                     /locus_tag="PKL26_06930"
     CDS             <1..1847
                     /locus_tag="PKL26_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNS83464.1"
                     /translation="ESLKIEGRMRSAEYVAAVVRAYRAALDALREGKEWRSTADMEEM
                     ATAFNRGFTGGYLLGERGPALMGRDRQDNRGLLLGCVLASPAKGGILVRTQAGYVPAA
                     GDGILIADPGGAVRSGYALNRDAVQHGDALFLPGVGGGAKPGSLVFLTRSMRLSARLK
                     ETLHAGSRRSPIPATLRVRIAEGLPLEGTATCPGPRGGRTEAKCTTGFIPPAIKNAPF
                     SKEAARRQLRKSGGTPFAVEAVDLDCRGDPFVPLGELNRLRRDILSSLQEAWLREFRP
                     EERLIDEAAARVQRFSQEHATKIAQVERMKKGDSIPSLTVYCATREEIAAACEGGCDS
                     VCYEPSTEEWAGYSGELAEGARYCREQRVSFTWKWPRITGPRFLKEALPLVPGLFRDG
                     LARVMVEEPGIAEAILSVEPGMEVIGGQGLNIYNAYAARAFHPPISSFTLSPELSGAQ
                     AAELIAISDRVTPGIQYEIFCQGSLEVIVSEDGLISTLAGSRKRKDNVAFGLRDETGR
                     LFPVYEDMMGRTRILNAVETTLIDRIPELLAMGIHSIAVDARGRGERYAGEMTRLYRE
                     GLGAAAGDGCSRDAMESLKDRARMMARGGLTSRHFDRGLAGYCDNEAE"
BASE COUNT          392 a          598 c          689 g          351 t
ORIGIN      
        1 tcgagtccct gaagatagag ggccggatgc gttccgcgga gtacgtcgcg gcagtggtcc
       61 gggcgtaccg ggcggccctc gacgcgctgc gggaggggaa ggaatggcgc tccactgcgg
      121 acatggagga gatggccacg gcctttaaca gggggtttac gggagggtat ctcctggggg
      181 agcgcggccc tgccctgatg ggaagggacc ggcaggacaa ccggggactc ctcctggggt
      241 gtgtcctggc atcgcctgca aagggaggta tcctggtccg cacccaggcg ggctacgtgc
      301 cggcagcagg agacggcatc ctcattgcgg atcccggagg agcggtgcgg agcgggtacg
      361 ccctcaaccg ggatgccgtg cagcacgggg acgcactctt cctgcccgga gtggggggag
      421 gtgcgaagcc tggctccctc gtctttctca ccaggagcat gcggctctcc gcaaggctga
      481 aagagaccct ccatgcggga tcacggaggt cccctatacc cgcgacactc cgggtccgga
      541 ttgccgaagg tctccccctg gaagggactg ccacatgtcc tggcccgcgc ggtggcagga
      601 cagaggccaa atgtaccacc gggttcatcc ccccggctat aaagaacgcc cctttttcaa
      661 aggaggctgc ccggagacaa ctccggaagt ccggcggcac acccttcgcc gtggaggcgg
      721 tggatcttga ctgccggggt gacccgttcg tcccgctcgg cgagctgaac cggctgagaa
      781 gggatatcct ttcctccctc caggaggcat ggctccgtga gttccggccg gaagaacggc
      841 tcatagacga ggcagccgcg cgcgtgcagc ggttctcgca ggaacatgct accaaaatcg
      901 cgcaggtgga gaggatgaag aaaggggatt cgatcccgtc actgaccgtg tactgtgcga
      961 cacgcgagga aattgcagca gcctgtgaag gcggatgcga ctctgtctgt tacgagcctt
     1021 ccacggagga atgggcaggg tactccgggg aactcgccga gggggccagg tactgccgtg
     1081 aacagcgggt ctcgtttacc tggaagtggc cacgcatcac gggaccccgg ttcctcaaag
     1141 aggcccttcc actcgtgccc gggcttttca gggatggcct ggcccgcgtg atggtcgaag
     1201 aacccggcat cgccgaagcg attctttcag tggaacccgg aatggaggtc atcggtgggc
     1261 agggcctgaa tatctacaac gcgtacgccg cacgggcgtt ccatcccccc atatcctcgt
     1321 tcaccctctc gcccgagctc tccggggcac aggcggcaga gctcattgcg atctcagacc
     1381 gggtgacacc tggcatccag tacgaaattt tctgccaggg gagccttgag gtgattgtga
     1441 gcgaggacgg gcttatctcc acgctcgcag gaagcagaaa aaggaaggat aatgtcgcat
     1501 ttggcctccg tgatgagaca ggcagacttt ttcctgtcta cgaggacatg atggggagga
     1561 cccgcattct gaacgcagtc gagaccaccc tgatcgatcg tattcccgag cttctggcca
     1621 tgggcatcca ctcaatcgcg gtcgatgccc gggggagagg ggagcgttat gccggtgaga
     1681 tgacccgcct ctaccgcgag gggctgggtg ccgcggccgg cgacgggtgt tccagggacg
     1741 ccatggagag tctgaaggac cgggcgcgca tgatggcgag gggcgggcta acctcccgtc
     1801 actttgaccg ggggctggcc gggtattgtg ataatgaagc agaataggtt ttcccctggg
     1861 ggatccatac cactggctga gagatccgta tgggaacccc attcatcctt ctcaacctga
     1921 agacatactc cgagggcagc ggccagcgtg cccaccacat cgccgaagcg gcccagcagg
     1981 tggccgagga gtcgggcgtg gagatcggga tcgccccaag ctacatgaac
//
LOCUS       DAOQVJ010000325         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_70717_length_2030_cov_4.367089, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000325 DAOQVJ010000000
VERSION     DAOQVJ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70717_length_2030_cov_4.367089"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKL26_06935"
     CDS             <1..190
                     /locus_tag="PKL26_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83465.1"
                     /translation="WFEAYLGGQWYTFDPRNNVPRIGRILIARGRDATDVAISNTFGP
                     NTLKSFKVWTDEVTGELP"
     gene            248..1243
                     /gene="purM"
                     /locus_tag="PKL26_06940"
     CDS             248..1243
                     /gene="purM"
                     /locus_tag="PKL26_06940"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNS83466.1"
                     /translation="MSSPHTYRDAGVDIGLEARAISALVQNLTFRRRGAYPMMGGVGH
                     FAGLTDFGDMALALTVDGVGTKMLVADRLKDWSTVGIDCMAMNVNDLYVMNMEPVAFV
                     DYIATDRLSVGQMAQIGEGLNEGARQANVNIVGGETATLKGLVNGLDLAGTCLGVQRK
                     ERVIAGEKIRPGDLVVGLPSSGIHSNGLSLARRVVESHDAWDETLSSGRSIGRELLVP
                     TRIYHEVLDLARETEIHGMCHVTGGGLLNFSRLTGFGFEFTDPMPPQEIFSWVQEHGE
                     VERAEMYRTFNMGMGFACIVPDGAEGPVLKRVRGSQVAGVVTARPGIYLEEEEIR"
     gene            complement(1249..1800)
                     /locus_tag="PKL26_06945"
     CDS             complement(1249..1800)
                     /locus_tag="PKL26_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HNS83467.1"
                     /translation="MRQVVPGPCPACGTEIEYIYQTEEIPYFFEILIISALCPTCGYR
                     LADTELLKNAEPSRWELTIESPADLDIRVVRSMNGVISIPELGVHVDPGPACEGFVSN
                     VEGVLNRIDAVLESVIGWTDDDEERRNAIQLREQIAEVKKGRLPVTLRIDDPTGNSAI
                     LGDRARIYPITAAECGYGPDTAE"
     gene            complement(1804..>2030)
                     /gene="sepF"
                     /locus_tag="PKL26_06950"
     CDS             complement(1804..>2030)
                     /gene="sepF"
                     /locus_tag="PKL26_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNS83468.1"
                     /translation="DSPRIKDEIYNGNIVIVDIAKLKADKVMYERVLKDLKEVAKDVN
                     GDIIGLGDQRYVVITPNSVKISRDKIGGVS"
BASE COUNT          396 a          595 c          618 g          421 t
ORIGIN      
        1 ctggtttgaa gcttacctgg gtgggcagtg gtacaccttt gatccacgca acaacgtccc
       61 gcgtattggc cgcattttaa ttgcccgcgg gcgcgacgcc accgatgtcg cgatcagcaa
      121 cacctttggt cccaacacgc tcaagagttt taaggtctgg accgacgagg tgacgggcga
      181 attaccgtga gctacctacc cctacaccct aagttgggcc aaccacagca gggaggccag
      241 gaggacaatg agtagccccc acacctaccg ggatgccggg gtggatatcg gcctggaggc
      301 acgcgccatc tccgcactcg tgcagaacct cacgttcagg cgaagggggg cgtatcccat
      361 gatgggcggc gtcggccact ttgccgggct gacagacttc ggcgacatgg cgctcgccct
      421 cacggttgac ggcgttggca cgaagatgct ggtggcggac cgtctcaagg actggtcgac
      481 agtgggaatc gactgcatgg ccatgaatgt caacgacctc tacgtgatga acatggagcc
      541 ggtggcattc gtcgactaca tcgccaccga tcgcctctct gtcgggcaga tggcacagat
      601 aggcgagggc ttaaacgagg gggctcggca ggcaaatgtg aatatcgtcg gcggggagac
      661 cgccactctc aaggggctcg tgaatggcct tgaccttgca gggacgtgcc tcggggtcca
      721 gaggaaggag agagtgattg ctggcgagaa gatccgcccc ggggacctgg tggtggggct
      781 cccctcttcc ggtatccaca gcaacggact ctcccttgcc cgccgggtag tggagagcca
      841 cgacgcatgg gacgagacgc tctcgagcgg caggagcata gggagggaac ttttagtgcc
      901 cacccgcatc taccacgagg tgctcgacct cgcgagagag acagagatac atgggatgtg
      961 ccacgttacc ggaggaggtc tcctcaactt ctcacggctg accggtttcg ggttcgagtt
     1021 cacggacccg atgcctcccc aggagatatt ctcgtgggtc caggagcatg gggaggtcga
     1081 gagagccgag atgtaccgga cgttcaacat gggaatgggc tttgcctgca tcgtccccga
     1141 cggtgccgaa ggcccggtcc tcaagagagt gagaggaagc caggtggcag gggtcgtcac
     1201 cgcccgtccc ggcatatacc tggaggaaga agagatccgt tgaacgtatt attcagccgt
     1261 atcggggcca tacccgcact cggcggcggt gatggggtag atccttgccc tgtctccaag
     1321 gattgccgag tttcccgtcg ggtcgtctat ccgtagcgtg acggggagcc tgcctttctt
     1381 tacttctgcg atctgctcgc gcaattgtat tgcgttcctc ctctcctcat catcgtctgt
     1441 ccagccgatc acgctctcaa ggaccgcgtc gatccggttc agcaccccct cgacgtttga
     1501 gacaaagccc tcgcaggccg gaccggggtc gacgtgcacg ccaagttccg ggatgctgat
     1561 gaccccgttc atgctccgga ccaccctgat atcaaggtcg gcgggtgact cgatagtaag
     1621 ttcccaccgc gaaggttcgg cgttcttcag aagctctgtg tctgcaaggc ggtatccgca
     1681 ggttgggcac agggccgata tgatgaggat ctcaaaaaag tagggaatct cttctgtctg
     1741 gtagatgtat tctatctctg ttccgcaggc cgggcaggga ccgggtacca cctgccgcac
     1801 tcttcagctg accccgccga tcttgtccct ggagatcttc accgaattcg gggtgattac
     1861 gacgtaccgc tggtctccga gacctatgat atccccgttc acatccttgg ccacttcctt
     1921 gagatccttg agcacacgct cgtacatgac cttgtcagcc ttcagcttgg cgatatcgac
     1981 gatcacgata ttcccgttgt agatctcgtc cttgatccgc gggctgtcct
//
LOCUS       DAOQVJ010000326         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_70816_length_2028_cov_5.317283, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000326 DAOQVJ010000000
VERSION     DAOQVJ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70816_length_2028_cov_5.317283"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..654)
                     /locus_tag="PKL26_06955"
     CDS             complement(286..654)
                     /locus_tag="PKL26_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83469.1"
                     /translation="MSWQDLALTSFIFLAGVLLIPQLLDTMNRGAVVNFFSASLTSVL
                     LFCISSVFASLGLWISVIAQSFVAVVWVCLAFFSLRNVRNSQFPDKSLFFVARDFLGV
                     WIFGVTFLVSNGARRLLRRD"
     gene            complement(983..1627)
                     /locus_tag="PKL26_06960"
     CDS             complement(983..1627)
                     /locus_tag="PKL26_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNS83470.1"
                     /translation="MTPIPWETPQDPLTQSSAGAMTGSAGEIRRPCSMAGAFLFSASV
                     LFMLGEVVLVAWYPGYLITENFISDLGVGPTATGFSVMLITCGVLIMAASWLLHTHST
                     SQSIPLLLFASGAGGAGVGIFPETLGAIHFICAGIAFLSGGVAAVLLSSRLHGPFRLF
                     SAILGVLSLFFIVSHILHLYFSLGPGGSERLIAYPLFLWMAGFGGHLMADRRAS"
     gene            complement(1624..>2028)
                     /locus_tag="PKL26_06965"
     CDS             complement(1624..>2028)
                     /locus_tag="PKL26_06965"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNS83471.1"
                     /translation="LGIRESRTFSINVPDASMIREVDYCGLVSGSNVDKSALFSVFYG
                     ETGTAPMIRECPLCLECRLVGSMQHTTNEFFVGEIVASWTSERFMTEGKLDIRKMNPL
                     LLTMPDNRYWTVGEEVGAAWSAGRDLARDGEK"
BASE COUNT          455 a          590 c          588 g          395 t
ORIGIN      
        1 tttcagccag ctcctcgcag gagaacgccg agaaatatct gccctccggt atcatgtgcg
       61 tctcctttta tttctatctt aagacctctt aaatggtgag ggattgcgcg ttcaaagatt
      121 cggaatgcca gccccagggc ttcttcgcat ggttgcgaag gcacaggagg ggtgcttctt
      181 ccctgtctcg catgcgtggc attcggattc gcaccttccc ccctcttctg cagcaagcgc
      241 ccgcctgttt aaaacaaaag cccgggcctg tttcccggtc ccggatcagt cccttctcag
      301 gagcctccgg gcgccgtttg agaccaggaa tgtcacaccg aatatccaga caccaaggaa
      361 atccctagcc acaaagaaga gggatttgtc ggggaactgt gaattccgga catttctaag
      421 ggagaagaac gcaaggcaca cccagacgac cgcgacaaac gactgtgcaa tgacggatat
      481 ccagaggcca agcgatgcaa agacagaact gatgcagaac aggagcacgc tcgtcaggct
      541 tgcggagaag aaattgacca ctgcgccacg gttcatcgtg tccagcagtt gcgggatgag
      601 cagtactccc gcgaggaaaa tgaagctcgt gagtgccagg tcctgccagc tcataaggaa
      661 ctcctgtgat gtcccatttt gcagaataag catcgacgtc aatgcatatc aaaagttaca
      721 tcctcctttt ccctgaaaga tgcgcgtcca gcggcaaaga ggggttatca ggctacgcga
      781 tagtcacgcc tgcctaccca gtagtgaggc atgtcgcccg gtcttttaag gggcgttccc
      841 gggtggtgat cagttcctcc tcatctgccg gagtggggga atgatccgca cgatagtgag
      901 gcttcccgcc ccctggaggc cgtagccctt ccccttgggc cccggtgccc tgccatccgg
      961 tggtgccatg cgtggttcct ggtcaggaag ccctgcgatc ggccatgagg tgccctccaa
     1021 acccggccat ccagaggaag agcggatagg cgatgagccg ttcggagccc ccggggccga
     1081 gcgagaagta gaggtggagg atgtgggaga cgatgaagaa gagagagagt acgccgagga
     1141 tggcggagaa cagcctgaat ggcccatgca ggcgggatga gagcagcacc gccgcaactc
     1201 cccctgacag gaatgcgatt cctgcacaga tgaagtggat agcgcccagg gtttcgggga
     1261 atatccctac acccgctccg cctgcaccgg aggcaaagag aaggagaggg atgctctggg
     1321 atgtagagtg agtatggagc agccaggagg cagccatgat caatactccg caagtgatta
     1381 gcatcaccga aaagccggtt gctgtcggcc ccactccgag gtcactaatg aagttctccg
     1441 tgataaggta cccgggatac catgcaacca ggaccacctc gccgagcatg aacagaaccg
     1501 aggctgagaa gaggaatgct ccggccatgg aacagggcct gcggatctcg ccggcactcc
     1561 cggtcatcgc acctgccgaa gactgcgtga gagggtcctg gggggtctcc caggggatgg
     1621 gtgtcatttc tcaccatccc gtgccaggtc tcttccggca ctccatgccg ctccgacctc
     1681 ttcgccgact gtccagtacc ggttgtctgg catggtcagg aggaggggat tcatcttcct
     1741 gatatccagt ttcccttccg tcatgaaccg ctcgcttgtc caggacgcaa cgatctcccc
     1801 cacgaagaat tcgtttgtcg tgtgctgcat ggaaccgacc agccggcatt cgaggcagag
     1861 ggggcattcc cggatcatcg gggcggtccc ggtctcccca tagaagaccg agaagagcgc
     1921 cgacttgtct acgttgcttc ccgacacaag cccgcagtaa tccacctccc tgatcatcga
     1981 tgcatccggg acgttgatgc tgaaagtccg actctcccgt atcccaag
//
LOCUS       DAOQVJ010000327         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_70904_length_2027_cov_2.850406, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000327 DAOQVJ010000000
VERSION     DAOQVJ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70904_length_2027_cov_2.850406"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="PKL26_06970"
     CDS             complement(<1..1884)
                     /locus_tag="PKL26_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNS83472.1"
                     /translation="MPGGVIRQGIALLGVILMVAFFLIAPGCAEGTNQGTLPPDAVDN
                     RGYTAEAPNGENPGYYLIDPATLPLVINNSMVAAHGTTYVLGGNWTQPAGCTASAVRI
                     TAHTNGLLLYGNNHTLTGSGSDYSAIVVDADNVTVSSVKIKGWGGAGIYLNPSTNVKV
                     ISNDVSGTGKRGIRSDRCVDVEITGNSLTNTSGIMADNSTNVKSSENVVVKNRGGIEY
                     YNVTRGEIRDNTVENSTYAGIRTQSGTRQITIMNNRISRNPFGIYLNTTSSNLTIYNN
                     LLNNTANCLIGDGVSGITWNSTRTPGPNIMGGPETGGNCYQLPGGDGFSQVIADSNED
                     GFCDAANTIGTGNIDHLPLAIPSLTITTPTGGEHWTQGSTQNVSWHYTSNPGMTVKIE
                     VLRGTTLLATIPSIPRGTAGTGSYPLTFPYYTPLGTDYKVRITSTSSPFITDTSEGPF
                     TIQPALMVISPNGGEVWQPGSTQTLRWKYTGTPGDTVKIEILRSGAPLATLASSVPIG
                     TGGNGSQPFTFPSYTPLGTDYQIRVTSTTYPACTDTSDGYFTISRLPSITVTAPNGGE
                     TWPQNSTQTITWSYTEDPGSMVSIELLNGTTVQQVITAATSIGTGGSGSYGFAIPTSL
                     PMGS"
BASE COUNT          395 a          542 c          603 g          487 t
ORIGIN      
        1 agatcccatg ggcaggctgg tggggattgc aaagccgtat gatcctgagc ctcctgtacc
       61 tatggacgtt gcagcggtga taacctgctg cactgtggta ccgttgagca gctcaattga
      121 taccattgaa cccggatcct cggtgtagct ccacgtgatg gtctgggtgg aattctgcgg
      181 ccaggtctca ccgccgttgg gggcggtcac ggtgattgag gggagccggc tgatggtaaa
      241 gtagccatcg ctcgtgtcgg tgcaggcagg gtaggtagta ctggtcaccc ggatctggta
      301 gtcagtgccg aggggcgtat aggaagggaa cgtgaacggc tgcgacccgt tccctcccgt
      361 gcctatcggg acactggatg caagcgttgc aaggggggct ccactcctca gtatctcgat
      421 ctttaccgtg tcacctggag tgccggtata tttccacctg agggtctgcg tggagccggg
      481 ctgccagacc tccccaccat tgggagatat caccatgagc gccggctgga tcgtaaaggg
      541 tccttcgctc gtgtcggtga tgaaggggct gctggtgctg gttatccgga ccttgtaatc
      601 ggtcccgagg ggggtatagt aggggaaggt aagaggatag gatccggttc ctgccgtccc
      661 tctgggaatg gacggaatgg tggcaaggag ggttgttccc cggagcacct cgattttcac
      721 cgtcattcct ggattgctcg tgtagtgcca gctgacgttt tgggtcgaac cctgagtcca
      781 gtgctcgccg ccggtgggag tggtgatcgt gagagatggg atggccagcg ggagatgatc
      841 gatgttacct gtccctatcg tattggcggc atcacagaac ccgtcctcgt ttgagtcagc
      901 aatgacctgt gagaatccgt caccgcctgg taactggtag cagttaccgc cagtctctgg
      961 tcctcccatg atattgggtc ctggcgtacg ggtactgttc caggtgatgc ccgacacccc
     1021 gtcaccgatg agacagttgg ccgtgttgtt gagcaggttg ttgtatatcg tcagattgga
     1081 cgaagtagta ttgagataaa tcccgaaggg atttctggat atccggttgt tcatgatagt
     1141 gatctgccgg gtcccgctct gggtcctgat gccggcatag gtgctgtttt cgacggtatt
     1201 atccctgatc tcaccccgcg tcacgttata atactctatc cctccccggt ttttcaccac
     1261 gacgttttcg gatgacttca cattcgtaga attatccgcc atgatgccac tggtattcgt
     1321 gagggaattt ccggttatct cgacgtccac gcaccggtcg gaacgaatgc ccctctttcc
     1381 cgtcccggac acatcattcg agatgacctt cacattcgta ctggggttga ggtagattcc
     1441 tgccccaccc catcccttga tttttaccga ggagacggtc acattgtccg catcgacgac
     1501 aattgcagaa tagtccgaac cggaaccggt aagggtatga ttattcccat acagcagcag
     1561 tccattcgta tgagcggtga tccggacagc actggccgtg cagcctgcgg gctgtgtcca
     1621 gttacccccg aggacgtagg tggtgccatg agccgcgacc atcgagttgt tgataacgag
     1681 gggcagggta gccggatcga tcaggtaata gccagggttt tccccgttgg gggcctctgc
     1741 tgtatatccc ctgttgtcaa cggcatccgg aggcaaggtc ccctgattcg tcccctctgc
     1801 acatccaggt gcgatcagga aaaacgccac catcagaatg acgccgagca gtgcgatacc
     1861 ctgccggatc actccacccg gcatggcatg gttaggtgtt ggatatcgtg tcaatcgtat
     1921 caccagggat actatcccat tccttccctg tcagtccagg tccggctctg cgcatcctga
     1981 aaccgtccat ggtgagagaa gagatcgcaa tgaacgcaca cgtaccg
//
LOCUS       DAOQVJ010000328         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_71793_length_2011_cov_3.514315, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000328 DAOQVJ010000000
VERSION     DAOQVJ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71793_length_2011_cov_3.514315"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKL26_06975"
     CDS             complement(<1..851)
                     /locus_tag="PKL26_06975"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNS83473.1"
                     /translation="MQSSQRTVGNLSLEYAIRKANKFKIPVVACFCIDPFYPEANLRH
                     YTFMAEGLVSAGRSLENMQIPLAIFWGVPGESIAGAAKDAAMVVTDRGYLSLHQTWRK
                     DLAGMVDCPLVMVEDNAVVPVETVSGKEEWSAGTIRKKIHRQLMRFLEPVERTPVQYP
                     SPSPEHEGIPYKEAETVITRVKEKNPGPSPLYRGGEHEALLRLEKFLEGNLPRYPSGQ
                     SDPATPVISGLSPYIHFGQVSPAYIAQRVLESRQPGADAFLEQLIIRRELSLNFVHFN
                     PHYDRYEG"
     gene            complement(1051..1392)
                     /locus_tag="PKL26_06980"
     CDS             complement(1051..1392)
                     /locus_tag="PKL26_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83474.1"
                     /translation="MPDRRNTPVFLVISLILIGIMVLPAVSAEGLAGPWISSSTSISG
                     NAVVNESASLKMVTYTGSIPVKQEWKFQNTFSPVQGNTWRSDLFFRPTENTLTFQYSR
                     LGLFSPVYLRV"
     gene            complement(1504..>2011)
                     /locus_tag="PKL26_06985"
     CDS             complement(1504..>2011)
                     /locus_tag="PKL26_06985"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNS83475.1"
                     /translation="LILIDQHAAHERILYEQVIDAGEREPECQELIVPVSLHLSPREA
                     AILPALFPLLEEIGFSIENFGRGTYAVRAIPIVLGKQIGQEGVREVVDALLSGDVRQD
                     SSEKERVLKVIACRGAIKGGTPLNVEQCRSLIAQLRRTEHPFSCPHGRPTMVTFKRRE
                     LDGLFFRT"
BASE COUNT          441 a          581 c          547 g          442 t
ORIGIN      
        1 ccctcgtacc tgtcgtaatg gggattgaag tgcacaaagt tcaacgagag ttcgcgccgg
       61 atgatcaatt gctccaaaaa cgcatccgca cccggctggc gggattcaag caccctctgc
      121 gcaatatacg caggagatac ctgcccgaaa tggatgtacg ggctcaaccc agagatgacg
      181 ggggttgcag ggtcgctctg cccagaaggg taccggggaa ggttcccttc gagaaacttc
      241 tcgaggcgca ggagtgcctc gtgctcccct cccctgtaca acggcgatgg tcccggattc
      301 ttctctttca cacgggtgat cactgtttct gcttccttgt agggaattcc ttcatgttcc
      361 ggcgaaggtg aggggtattg gaccggcgtg cgttcgactg gctcgagaaa acgcattaat
      421 tggcgatgga tcttcttccg gatcgtgccc gccgaccact cctccttgcc agatactgtc
      481 tccacgggga cgaccgcgtt gtcctccacc atcacaaggg gacagtccac catccccgcc
      541 agatcctttc tccaggtctg gtgcagggag aggtaccccc ggtccgtcac gaccatcgca
      601 gcgtctttgg cagcccctgc gatactctcc ccgggcactc cccagaagat cgcgagaggg
      661 atttgcatat tttcgaggga tcttcctgcc gaaaccaggc cttctgccat aaacgtataa
      721 tggcggagat ttgcctccgg ataaaaggga tcaatgcaaa agcaggcgac gacgggtatt
      781 ttgaatttgt ttgccttcct tatggcatac tcaagggaaa ggtttcccac tgtccgctgc
      841 gaggactgca tccagtagag gacatattct cccgaacgcg caggaagcgc gttgagggcg
      901 catatgcgct cgccggggat catgcatcag gacaatggta ttcccaccta catggaacct
      961 tctcttccgg catcctgcag gtgccttgaa atatcagaaa tcagggaaca acatcctgcc
     1021 agtcacaggg gagtgcccgc agggaaaaaa ttatacccgg aggtatacgg gggagaaaag
     1081 ccccaacctc gagtactgga acgtcagcgt gttctctgtc ggcctgaaga agagatccga
     1141 tctccaggta ttcccctgca caggggagaa agtgttctgg aacttccatt cctgcttcac
     1201 agggatactg cctgtgtacg tgaccatctt gaggctcgca gattcgttca cgactgcatt
     1261 gcccgagata ctggtgctgc tcgagatcca tggcccggca agcccctctg cagagactgc
     1321 tggcaggacc ataatcccga ttaaaatgag gctgataacc aggaatacgg gcgtatttct
     1381 tctgtctggc atgattatcc tccgtgtggc gtggcggcgc acaacggaac atgttcgcac
     1441 tagtatcgga ccctttcacc tatatgcttt cctctcaacc gataaaaaaa cgcgaacttt
     1501 cattcaggtt ctgaaaaaca ggccgtcgag ttctcttctt ttgaaggtga ccatcgtcgg
     1561 cctgccatgg gggcagctga agggatgctc agtccggcgc agttgcgcga tcagcgacct
     1621 gcactgctcc acgttcaggg gagtcccccc cttgatggcc cccctgcatg cgattacttt
     1681 gaggacgcgc tccttctcgg atgaatcctg gcgcacgtcc ccggagagaa gggcatcaac
     1741 aacctcccgg acaccttcct gcccgatctg ctttcccaga acgatcggaa tggcacggac
     1801 agcatacgtt cctctcccga agttctctat ggagaacccg atctcttcaa ggagggggaa
     1861 aagagccggg aggattgcag cctcgcgcgg ggacaggtgc aatgaaacgg gcacgatcag
     1921 ctcctggcac tcgggctctc tttcccccgc atcaatgacc tgttcgtaca ggatacgctc
     1981 gtgtgccgca tgctgatcga tcaggatgag g
//
LOCUS       DAOQVJ010000329         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_72103_length_2006_cov_3.620707, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000329 DAOQVJ010000000
VERSION     DAOQVJ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72103_length_2006_cov_3.620707"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(526..768)
                     /locus_tag="PKL26_06990"
     CDS             complement(526..768)
                     /locus_tag="PKL26_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83476.1"
                     /translation="MKKGFQQHNSKDIRLTLYHNGKKTRIVTWVSHGTKEIGDKLIGI
                     MARQLRIEKSEFEDLVDCTLSGQGLLDIYLDRGIRF"
     gene            complement(808..1854)
                     /locus_tag="PKL26_06995"
     CDS             complement(808..1854)
                     /locus_tag="PKL26_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83477.1"
                     /translation="MIRSDREIELFITSNDKTHPEVSVNVLISNIQAVQNLMYIMGDH
                     LEGHQARTAGDFPRSVKENCELVISGLEMGSAAITMRILHDQYGLPGAGGTYGERAIS
                     LVGTVIDIASNQENILPEVIELLQSDEHRAIRSVQEVESLWPDEGSAYDVALRFGSSG
                     TIRLDRTRKPMIKNALMRSPEPAEKTISGRMVELSVDRKRQFVLDTPEGKYTCRYPPE
                     LEESVIRRIKQLVSISGSIGAASRTINIESEIAFKPLESLPLTHIRLDGDLRRLRYPV
                     DLEVEFEDGEYILYNNEFNLLVVSDNLKDGVERITDELRLLWLDYVGEEEANLTEGGV
                     AFRNKLRSLLGEAI"
BASE COUNT          423 a          594 c          480 g          509 t
ORIGIN      
        1 ctccgccggg gagatggcga gggggccctg agaagagcaa gtgttccggc cggcactacc
       61 cttgagcggc cagttcggtg gtgaccgatt cgagcagttt tgcctgtgct tcctctcact
      121 cggcgatccg tgcctcgagg gcatcgaaga gtttcatgag agcatcggcc ttcgagaact
      181 gatgtttatc caacacccat ccaggtttac gtagtcgttc tcttcacatt ccagtttatc
      241 ccccggaata cagttggaaa tgagagcaca actatccaac cgagatgcca acaactcggc
      301 tctattcccc atcgctgatc tcagtgggga tccacctcgt ggagcaatcc cgggcgggga
      361 cgtttaggta agaatcgatg cgttgtccat ccccatgtaa atcctgcaga aaggtggatt
      421 cgagatcatt gcgggatgcc cttcacccgc ttattcaggg cggtccagaa ccctgatcgt
      481 ttgttccagg ctccatctcc ggtcccggcc ttccgctggc gcatctcaaa accggatccc
      541 gcggtcaagg tagatatcga gcaaaccctg cccggataac gtgcaatcga cgagatcctc
      601 aaactcgctc ttctctatcc tcaactgcct tgccatgatg cctatcaatt tatcgccgat
      661 ctcttttgtc ccatggctga cccaggtcac gatccgcgtc ttcttcccgt tatgatagag
      721 ggtgagcctg atatcctttg aattatgctg ctggaatccc ttcttcaaca gagatctctg
      781 gatcgtccgg ttttttcgca ttcccaacta tatcgcctca cccagcaacg aacgcagttt
      841 gttgcggaat gcaacccccc cctctgtcaa attcgcctcc tcctctccaa catagtccag
      901 ccagagcagc ctgagttcat cggtaatccg ttctacccca tccttcaggt tatcagatac
      961 gaccaggagg ttgaactcgt tgttgtacag tatgtattca ccatcctcga actcaacctc
     1021 caggtcaacg ggataccgca gcctgcggag atccccatcc aacctgatat gagtgagagg
     1081 gagggattca aggggcttaa acgctatttc actctctata ttgatggtgc gcgatgccgc
     1141 tccgatcgat ccgctgatgc tgacgagttg cttgatcctc ctgatgacag attcttctaa
     1201 ctccggaggg tatctgcacg tgtactttcc ttcgggagta tcgaggacga attggcgttt
     1261 cctatccacg ctcaactcca ccatacggcc cgatattgtc ttctcagccg gttccggcga
     1321 tcgcatcagg gcattcttga tcatcggttt tcttgtgcgg tccagcctga tcgtccctga
     1381 agagccaaac ctgagcgcaa catcgtatgc tgaaccctca tcaggccaga ggctctctac
     1441 ctcctgaacc gatcggatcg cccggtgctc atcactctgg agcaattcga taacttccgg
     1501 gaggatgttc tcctggttag atgcaatatc tataacagtg cccacaaggg agatcgcgcg
     1561 ctctccatag gtgccgcctg caccaggcag tccgtactga tcatggagga tcctcatggt
     1621 gatagcggcg ctacccattt ccagccctga tataaccagt tcgcagttct ccttcacgct
     1681 cctcgggaag tcccctgcag ttcttgcctg atgcccctcc agatggtctc ccataatgta
     1741 catcaagttt tgaacggcct ggatgttgct gataagaacg ttgacagaga cctcagggtg
     1801 ggttttgtcg tttgacgtga tgaagagctc gatctcccta tccgatcgga tcattcgcca
     1861 gactacctcc ggactgccga ttataatggt ggtctatgta tgaacattta tgcatatggg
     1921 taagcccgat gtcggcaccc ccggttgata tacgcgcggc gttaccccca ttgcacccca
     1981 aagattcctg cccccggaga tcgagt
//
LOCUS       DAOQVJ010000330         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_72816_length_1993_cov_4.461300, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000330 DAOQVJ010000000
VERSION     DAOQVJ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72816_length_1993_cov_4.461300"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKL26_07000"
     CDS             complement(<1..1212)
                     /locus_tag="PKL26_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNS83478.1"
                     /translation="MDQEEDRNAIYQEALALHEEHHGKLEIRSRVPLETRRDLSRAYT
                     PGVAEVCRAIQKDRDMAYRYTLKRNSVAIITDGTAVLGLGNIGGYAAIPVMEGKAILF
                     KKFAGIDAFPICFDGPQSDFVDQVRNIAPVFGGINLEDIAAPRCFEVEEGLQDLGIPV
                     MHDDQHGTAITVLAALLNACRATKKQFEDLHIVVCGAGAAGYAITRLLRCIGYSPDVC
                     RSVREIVVCDTQGIIHRARKGLYSNKYKFILADETNRSGRTGSLSDAMEGADVFIGVS
                     GPGVVNEEMIRLMNDEPIVFAMANPVPEIMPDAARRAGAAVVGTGRSDYPNQINNALA
                     FPGVFRGALDAFATRISDEMKVAAAHAIADYVPHPHREHILPTILDRDVTRAIAGAVK
                     GAAIQSGCARDL"
     gene            1321..>1993
                     /locus_tag="PKL26_07005"
     CDS             1321..>1993
                     /locus_tag="PKL26_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNS83479.1"
                     /translation="MAMSGKCPFHFVLLGIALGTLLAAAGCTVPVNVTLDPSGHPAYM
                     CGVPVDARVTRVIDGDTIAVKIAGGREETVRVLGIDTPETEEHGNWGNEYEGISNPSY
                     LTSWGHRASNETESLVKGKSVTLVADCMTGERDRYGRLLAYVGIEGSDLGSLLLQGGY
                     ARVYTEESFGKKQLYLLIQSEAQLAGTGLWSAAKTPESSQKSPVSIASVQYDAPGDDR
                     ENPNGE"
BASE COUNT          368 a          618 c          623 g          384 t
ORIGIN      
        1 aaggtccctt gcgcatcccg actggatcgc ggcccctttt accgcacctg caatcgcccg
       61 cgtcacgtcg cgatcgagga tcgtggggag gatgtgctcc cggtggggat gcggcacata
      121 gtccgcaatg gcatgtgccg ccgccacttt catctcgtcg ctgattcggg tggcaaaagc
      181 gtcgagcgcc ccgcggaata cgccggggaa tgcaagggca ttgttgatct ggttggggta
      241 atcgcttcgc cccgtcccca ccaccgctgc accggcacgc cgggcggcat ccggcatgat
      301 ctctggaaca gggtttgcca tggcaaagac gatcggctca tcgttcatca gacggatcat
      361 ctcctcgttg accacgccgg gcccggagac cccgatgaag acgtctgctc cctccatcgc
      421 atccgacagc gatcccgtcc gcccggagcg gttggtctcg tctgccagga tgaacttgta
      481 cttgttgctg tagagtcctt tccttgcgcg gtgtatgatc ccctgggtat cgcagactac
      541 gatctcgcgg acgctccggc atacgtccgg gctgtacccg atgcagcgga ggagccgggt
      601 gatggcatag cctgcagcac cggccccgca gactacgatg tggaggtcct cgaactgctt
      661 cttcgtggcc cggcaggcgt tgagaagggc cgcgagtacc gtgatagcgg tgccgtgctg
      721 gtcgtcgtgc atcacaggga tgccgagatc ctgcaggccc tcctccacct cgaagcaacg
      781 gggggcggcg atatcctcca ggttgatccc cccgaagacc ggggcgatgt ttctgacctg
      841 gtccacgaag tcagactggg gaccgtcgaa acagatggga aacgcgtcaa ttccggcaaa
      901 cttcttgaag aggatggcct ttccctccat tacagggatg gcggcatacc ccccgatgtt
      961 cccgaggccg agcaccgctg tcccgtctgt gatgattgcc accgagttcc gcttgagggt
     1021 gtagcggtat gccatgtccc tgtccttctg gatcgccctg cagacctctg ctacgccggg
     1081 agtataggcc ctggagaggt cgcggcgggt ctcgagcggc acccttgatc gtatctccag
     1141 ctttccatgg tgctcctcgt ggagcgccag cgcctcctgg tagatcgcgt tacggtcctc
     1201 ttcctggtcc atgattccat caaggtcgcc tcatcacata aaacccgggt ctttcctcaa
     1261 gccacgtgaa ttcaaacccg ggcggcccgg caacacttat ttcgatgcac gacgaccaga
     1321 atggctatgt ccgggaaatg cccctttcac ttcgtgctcc tcggaatcgc actcggcact
     1381 ctcctcgccg ccgctggatg cacggtgccg gtgaacgtca cgctggatcc ctcggggcac
     1441 cctgcctaca tgtgcggggt accggttgat gcccgggtca caagggtcat cgacggcgac
     1501 accatcgcgg tgaagattgc cgggggaagg gaggagaccg tgcgtgtgct cggtatcgac
     1561 acgccggaga ccgaggagca tgggaactgg ggcaatgaat acgaggggat cagcaatccc
     1621 tcctacctga catcgtgggg gcaccgggcg agcaacgaga ccgagagcct ggtgaaaggg
     1681 aagagcgtaa cgctcgtggc cgattgcatg accggcgagc gcgaccggta cggccggctt
     1741 ctcgcgtacg tcgggatcga gggatccgac cttgggtccc tgctcctcca gggcgggtat
     1801 gcgagggtct acaccgaaga atcgttcggg aagaagcagt tgtacctcct gatccagtcc
     1861 gaagcacagc ttgccggcac ggggctctgg agtgcagcaa agaccccgga gagttcgcaa
     1921 aaaagcccgg tgtcgatcgc ttctgtccag tacgatgccc ccggcgacga cagggagaac
     1981 ccaaacggcg agt
//
LOCUS       DAOQVJ010000331         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_73053_length_1989_cov_3.740434, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000331 DAOQVJ010000000
VERSION     DAOQVJ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73053_length_1989_cov_3.740434"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKL26_07010"
     CDS             <1..677
                     /locus_tag="PKL26_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS83480.1"
                     /translation="VGRLEGDGAVSGAMLKTGAKENTVSCDGVFLAAGLVPNSGMVSG
                     LKKGQDGRILVDRSMVTSNPSVYACGDVTGSRALTPVARYEGTVAAASILGREPPPDC
                     TPIPQSMNLASEYAFVEADGSDDEVAFGTPGPAGPGTFWSVPRGHTGMAGILVSRESG
                     KVGAVYTAGPAAGILAAYHSFLMRLGVNAHDFKWFMEVHPMTDGVYPLMKYASFRLHQ
                     EKEDRP"
     gene            complement(697..>1989)
                     /locus_tag="PKL26_07015"
     CDS             complement(697..>1989)
                     /locus_tag="PKL26_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNS83481.1"
                     /translation="MPGVMETILNIGLSRETVRGLISLSGNPKFAWDSYRRLIQNFGQ
                     VVCRQPPALYQAALRQAMEREGVEDEVELDAGSLRDLAVEYERIFAQNGGEKFPAGAM
                     RQLEQAAEAVILSWESPRAEAFRRLNLIREARGTAVTVQAMVFGNVGSRSGAGVAFTR
                     NPWQGNAQLLVDFKFGAQGEDVVSGDQSISTQHEFQRALPETFLELSDTAKQLETYYK
                     DMQDLEFTVQNGELYILQTRSGKRSPYAALKVAVDLCQEGIISPQDVIWMLREVDLDA
                     ISVERVRTRDHPLAVGIPASGGVAAGDIAFSGASAETMESEGRNVILVRETPSPDDIP
                     GIRAAIGLLTARGARTSHAAVVARQMGRVCVVGCESLVIDEARRRCRISGQELREGAR
                     ITLDGNSGKVFRGEVEYSSERPTALIAMVREWEQSIHL"
BASE COUNT          343 a          663 c          608 g          375 t
ORIGIN      
        1 cggtcggcag gctggaggga gatggcgcgg tcagcggtgc catgctgaaa acaggcgcaa
       61 aagagaacac ggtttcctgc gacggggtct tccttgccgc gggactggtc ccgaactccg
      121 gcatggtctc cgggttgaaa aaagggcagg acgggcggat cctcgtcgac cgctcgatgg
      181 tcaccagcaa cccctccgta tacgcatgcg gggacgtgac cggctcacgt gcgctcacgc
      241 ccgtggcacg gtacgaaggc acggtcgccg cagcgtcaat ccttggccgg gaacctcccc
      301 ctgactgcac gcccatcccg cagtccatga accttgcatc ggaatacgca ttcgtggaag
      361 cggacgggag cgatgacgag gtcgcatttg ggaccccggg gcccgcgggg ccggggacgt
      421 tctggtcggt cccacggggg cacacgggga tggcgggtat cctcgtctcg agagagtcag
      481 gaaaggttgg cgcggtgtac accgccgggc ctgctgcagg gatccttgct gcctaccact
      541 cgtttttgat gcgcctcggg gtcaatgccc atgatttcaa atggttcatg gaggtccacc
      601 ccatgacaga cggggtatac cctctgatga agtatgcctc gttccggctt caccaggaga
      661 aggaagatcg gccctgaccg ggtcatacgc tgccgctcac aggtgtattg actgttccca
      721 ctcccgcacc atcgcgatga gcgcggtggg gcgttcagac gagtattcga cctcccccct
      781 gaacaccttc cccgaattgc cgtcgagggt tatccttgca ccctcgcgga gttcctggcc
      841 gctgatccgg caccgccgcc tcgcctcgtc gatgaccagg ctctcgcacc ccaccacgca
      901 gacccggccc atctgccttg ccaccaccgc ggcatgagag gtcctggcgc ccctcgcggt
      961 gaggagaccg attgccgccc ttatcccggg gatatcatca ggggaaggtg tctccctgac
     1021 cagtatcacg tttctccctt ccgattccat cgtttcagcg ctggcgccgg agaacgcgat
     1081 atcccctgct gcgaccccgc ccgacgcggg aatcccgact gcgagggggt ggtcccttgt
     1141 cctcacgcgt tcgacagaga tcgcatccag gtcaacctcg cgaagcatcc agatcacgtc
     1201 ctggggtgag atgatccctt cctgacagag gtctaccgcg accttcaggg ccgcgtaggg
     1261 ggagcgtttc ccgctccttg tctgcaggat gtaaagctcc ccgttctgga ccgtgaactc
     1321 gaggtcctgc atgtccttgt aataggtctc gagctgcttt gcggtatcag agagttccaa
     1381 aaaagtctct ggcagggccc gctggaactc gtgctgggtg ctgatcgact ggtctcccga
     1441 gaccacgtcc tcaccctgtg ccccgaactt gaagtccacc agcagctggg cattcccctg
     1501 ccaggggttt ctcgtgaagg caacgcccgc acccgagcgg gagcccacgt tgccgaacac
     1561 catcgcctgg accgtaaccg cggtcccccg ggcttccctg atgaggttga gccgtcgaaa
     1621 ggcttcagcg cgtgggctct cccacgagag gatgacggcc tcggccgcct gctcgagctg
     1681 ccgcatggcc cctgcaggga acttctcgcc tccgttctgg gcaaaaatcc gctcgtactc
     1741 aacggcaagg tcgcggaggg atccggcgtc aagctcgacc tcgtcctcga ccccctcccg
     1801 ctccatcgcc tgccggagcg ctgcctggta gagcgcgggg ggctggcggc acaccacctg
     1861 cccgaagttc tggatgagcc tccggtacga gtcccatgca aattttgggt tccccgagag
     1921 ggagatgagc ccccggacgg tctcccggga gagcccgatg ttcagtatgg tctccatcac
     1981 tcccggcat
//
LOCUS       DAOQVJ010000332         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_73282_length_1985_cov_3.962694, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000332 DAOQVJ010000000
VERSION     DAOQVJ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73282_length_1985_cov_3.962694"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..76)
                     /locus_tag="PKL26_07020"
     tRNA            complement(5..76)
                     /locus_tag="PKL26_07020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:ccg)
     gene            complement(213..1241)
                     /gene="hypD"
                     /locus_tag="PKL26_07025"
     CDS             complement(213..1241)
                     /gene="hypD"
                     /locus_tag="PKL26_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNS83482.1"
                     /translation="MALGKEISEKLRECADRDLSFMHICGTHEAAISRSGLRSMLPPG
                     LKIVMGPGCPVCITPQGEIDAALDLVGKGCIVATYGDLLRVPGSRGSLESCGGDVRVV
                     QGVHKAVEIARNTTREVAFISVGFETTAPTVAATLLTRPPENFSILSCHRLVPPAMKW
                     LLEQGEAKLHGFLLPGHVCTVVGYQDYEQFPVPQVVAGFEPEDILLGLLMLVEQVRAG
                     EAKVENAYPRAVTREGNTRARQLMYEVFTPVDAEWRGFPVIPRSGLGLRPEFEQYDAK
                     KKFDLTFRHVEKHSACICDKVLRGIAEPTDCRLFGKACTPRTPIGPCMVSHEGACKIW
                     HTFRVKQK"
     gene            1312..>1985
                     /locus_tag="PKL26_07030"
     CDS             1312..>1985
                     /locus_tag="PKL26_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNS83483.1"
                     /translation="MNGNLLAVIVSITGLVIALAMYGIVGWFRKKAEQTESQLDDILL
                     AAFGTPAVVAVLVITFFLSLQVADLPAQFQFILDSKYVNVIWILLGAWIISTFIYTMI
                     ETYGHRIAAGTKTDLDDRMIALAQVASKYVIWFAAFLLVLYVLEIDITPFLAGAGIAG
                     IALALAAQDFLSNFFGGAIIAVDKPFKINDRIMIDQYVGDVICVGARSTRIKTLDNQI
                     VTFPNK"
BASE COUNT          393 a          587 c          582 g          423 t
ORIGIN      
        1 gggacgcccc agccgggatt cgaacccggg tcggaagctc cggaggctcc caggatatcc
       61 actaccctac cggggcgcgt gagagaagat accgcaacgt tacggaattg ctgcggttca
      121 taattatggc gcgtcttatc gtaaatacat ttgtcatcgg caatcggaag aatgaatgga
      181 acgcccggtt ctgacaatgc accgggctgc gcctactttt gcttgacacg gaacgtgtgc
      241 cagatcttgc aggccccttc gtggctgacc atgcatggcc cgatcggcgt ccgtggcgtg
      301 caggcttttc caaagagcct gcagtcggtg ggttctgcga ttccccggag gactttatcg
      361 cagatgcagg ccgagtgctt ctcgacgtgg cggaaggtga ggtcaaactt cttcttggcg
      421 tcgtactgtt cgaactctgg cctgagtccg agtcctgagc gcgggatgac cggaaagccc
      481 cgccactcag catcaacggg ggtaaacacc tcgtacatca gctgcctggc ccgggtattt
      541 ccttcccgtg tcacagcccg ggggtacgcg ttctcgactt tggcctctcc ggccctcacc
      601 tgttcgacga gcatcagaag gccgagcagg atgtcctcgg gttcgaaacc tgccaccacc
      661 tgggggacgg ggaactgctc gtagtcctgg tagccgacga ccgtgcagac atggcccggg
      721 aggaggaacc cgtggagttt tgcctcgccc tgctcgagga gccatttcat tgcagggggg
      781 accaggcggt ggcacgagag gatgctgaag ttctccggag gccgggtgag gagggttgcc
      841 gcaacggtcg gggcagtggt ctcgaacccg accgagatga atgccacctc tcttgtggtg
      901 ttcctcgcaa tctccacggc cttgtgcact ccctgcacca ccctgacatc gccgccgcaa
      961 gactcgagag aacccctgct tccggggact cgcaggaggt ctccgtaggt ggcaacgatg
     1021 caccctttcc cgacaaggtc gagcgcagcg tcaatctccc cctggggagt gatacagaca
     1081 gggcagccgg gccccatcac gatcttgagg cctggaggca gcatgctcct cagtcctgac
     1141 cgggagatgg cagcctcgtg ggttccgcag atgtgcatga aagagagatc cctgtcggcg
     1201 cactcccgca atttttccga tatttccttg ccaagggcca tatattggac caattatttt
     1261 gtatccacga gtgataatgg tactgatatg gcgactgaga caatattcgg aatgaacgga
     1321 aatctcctcg ccgtgattgt gagcatcacc gggctggtca ttgcgctggc catgtacggg
     1381 atcgtcgggt ggttccggaa aaaggccgag cagaccgagt cccagctgga tgacatcctg
     1441 ctcgcggcgt tcggcacccc cgcggttgtc gcggtgctgg tgatcacgtt cttcctctcg
     1501 cttcaggtcg cggaccttcc tgcccagttt cagttcatcc tggacagcaa atacgtgaac
     1561 gtaatttgga tccttcttgg ggcatggata atatcgacgt tcatctatac aatgatcgag
     1621 acatacggtc accgtatcgc ggccgggacc aagaccgacc tcgatgaccg gatgatcgcg
     1681 ctcgcgcagg tcgcgtcgaa atacgtgata tggttcgcgg cattcctcct tgtcctctac
     1741 gtcctcgaga tcgacatcac gcctttcctt gcgggtgcag gcattgccgg gatcgccctt
     1801 gccctcgcag cacaggactt tctctccaac ttcttcggcg gcgccataat cgcggtagac
     1861 aagcccttca agatcaacga ccggatcatg atcgaccagt atgtcggcga cgtgatctgt
     1921 gtcggcgccc gcagcacgcg gataaaaacc ctcgacaacc agatcgtgac gtttccgaac
     1981 aagaa
//
LOCUS       DAOQVJ010000333         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_73719_length_1977_cov_4.410510, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000333 DAOQVJ010000000
VERSION     DAOQVJ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73719_length_1977_cov_4.410510"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKL26_07035"
     CDS             <1..213
                     /locus_tag="PKL26_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNS83484.1"
                     /translation="GWCAKTRKLLDDLGVEYSYVYVDRLPHEEMESAIREVEKHNPSG
                     SFPTLVIDGSRAIVGFREADIREALK"
     gene            210..716
                     /locus_tag="PKL26_07040"
     CDS             210..716
                     /locus_tag="PKL26_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNS83485.1"
                     /translation="MTGEGIPEQTIRKKADKIRSDASAGGYLLNPDQSFVRELVAGIC
                     VNEQRYGYPACPCRLASGSREEDLDIICPCDYRDADLNEFGACYCALYVSAGIASGEA
                     QVTCVPERRPSRKERRKESRSAPVFAGELPEPVWRCRVCGYLCARKSPPLVCPICKAS
                     QDRFTRFI"
     gene            829..1176
                     /locus_tag="PKL26_07045"
     CDS             829..1176
                     /locus_tag="PKL26_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83486.1"
                     /translation="MADTISIEVVGLKDSTCSPFPCDMTRSCGLYDCHPTGKLVPAFE
                     ALKKELVSQYGESVRMTLVLIDEDMPDHVREIIATHYPPIPFILMNGALIPMGRISLP
                     LMQKEIEKILSIR"
     gene            complement(1169..1750)
                     /locus_tag="PKL26_07050"
     CDS             complement(1169..1750)
                     /locus_tag="PKL26_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNS83487.1"
                     /translation="MIDELLEGNKKFREGFFKENRAHHQELTKGQSPKVLWIGCSDSR
                     LQTGHITQTLPGTLFMHRNIGNIVPLQDWNFATVLEYAIRHLKVKDIVVCGHSDCGAI
                     KALDKETDDVYIPLWLNYAIEAKHRVDATLPAPKTPEEAKKRSRMIEKENIRLQIEHI
                     MQYPIVKMAIKEKKVQVHGLYYDLDNGELSRIT"
BASE COUNT          432 a          501 c          562 g          482 t
ORIGIN      
        1 ggctggtgcg cgaagacccg gaaactcctc gatgacctcg gggtcgagta ttcgtatgtg
       61 tacgtggatc ggctcccaca cgaagagatg gagagcgcga tccgggaggt tgagaagcac
      121 aatccaagcg gttcattccc caccctggtc attgacggtt ccagggctat tgtcggtttc
      181 agggaagccg atatccggga ggcgctgaaa tgacaggaga gggaattccc gagcagacca
      241 ttcgtaaaaa ggccgataag atccgatccg atgccagtgc aggaggttat ctcctcaacc
      301 ccgatcagtc ttttgtcagg gaactggttg cgggcatatg cgtgaatgaa cagcgttacg
      361 gttaccctgc ctgtccatgt cgccttgcct cggggagcag ggaggaggac ctcgacatca
      421 tctgcccatg cgattaccgt gacgccgatc tgaatgagtt tggtgcctgt tactgcgcac
      481 tgtacgtgtc tgcagggatt gcatcgggag aagcgcaggt aacgtgcgtc cccgaacggc
      541 gcccttcacg aaaggagagg agaaaggagt cgaggagcgc gcctgttttt gcaggcgagt
      601 tgccggaacc agtatggcgg tgcagagtat gcggctacct gtgtgccagg aaaagcccgc
      661 cgcttgtctg ccccatctgc aaggcttcgc aggaccggtt tacccgtttc atctagcacc
      721 tcaaagagga caccgggagt ccgggaactg gaggaaattg gtcacccggt cctgcgccat
      781 agcgcgatag cacacactaa tgtaggatac ttcccaaaac tgtacatcat ggcagatacc
      841 atttccatcg aagtggtggg gctcaaagac tcgacctgct caccatttcc ctgcgatatg
      901 acgaggagct gcgggctgta cgattgccat cccaccggaa agctggttcc cgcattcgag
      961 gccctgaaaa aagagctggt ttcgcagtat ggcgaatcgg tgcggatgac actcgtgcta
     1021 atcgatgaag acatgcctga tcatgtcagg gagatcattg ctacccatta tccgccaatc
     1081 ccattcattc tcatgaacgg ggcgttgatt cccatgggca ggatctcgct gccgttgatg
     1141 caaaaagaga tagagaagat actctcgatc aggtaatccg ggagagttcg ccattgtcga
     1201 ggtcgtaata aagaccgtgg acctggacct tcttctcttt tattgccatt ttcacgatgg
     1261 gatactgcat gatgtgctcg atctggagcc tgatgttctc tttttcgatc atccttgaac
     1321 gtttctttgc ctcttcaggg gtctttggtg ccggaagcgt tgcatcgaca cggtgtttcg
     1381 cctcgatggc ataattcagc cagagtggaa tgtagacatc gtctgtttct ttatcgagtg
     1441 ccttgatggc cccgcagtcc gagtggccgc agacaacaat atccttcacc ttgaggtgcc
     1501 tgattgcata ttccagcacg gtcgcaaagt tccagtcctg gagcggcact atgttcccga
     1561 tattcctgtg catgaagagg gtccctggca acgtctgggt gatatgtccg gtctggagcc
     1621 tcgaatctga gcatccgatc cagaggacct tcgggctctg acctttggtc agttcctggt
     1681 gatgtgccct gttctccttg aagaagcctt ccctgaattt cttattgcct tcgagcaatt
     1741 cgtcaatcat ggtatatgca cctgtgtcct tccttcttcg tcacgatgag cgccgatcat
     1801 gggagcagtc actcccgatc tggatatggt ggtgcaaagg gattaaaggt tgatattttg
     1861 ctctcggcgt tcataccata aaagaagaga atgtggttga gttgaaagct ctcccgggga
     1921 tgatccggaa agccggtgca gcgtcatcgc ggttctggga ggtagattac tgcaggg
//
LOCUS       DAOQVJ010000334         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_73873_length_1975_cov_3.681250, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000334 DAOQVJ010000000
VERSION     DAOQVJ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73873_length_1975_cov_3.681250"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PKL26_07055"
     CDS             complement(<1..1378)
                     /locus_tag="PKL26_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS83488.1"
                     /translation="MESQIWQPLPGVEGVSIYPFLRKVDIISSNSYLVAAPRLIVLVD
                     PGGLPGQADTLLGEIRKLITEKPRPVLICLTHVHLDHCHQLLHHPGFRTLPHMLVAVQ
                     EAGARALESRDRAATIADLIRQEVGTITIDIRLLPSGNGGEHRDEILNIAGSSVITCL
                     DTLPLGGGLTISRQTLPLEGDTMIEFYPAPGHSPDSICIRMGETLFLGDLLFSTAPGV
                     AGLKGWDLKALLFTLDQVIALLQKGGITVCLPGHGRPLDAATTTRTLQAMKKEASRLE
                     GIEIVNPEWVKDTAAYAGELMGEVDRLFTIIAGRLVYVAHVLDELEESGEAERFRGLI
                     NSDLVDEILSDFNRFSLDFHSGQALEIHLALKANQISQKLEKVYRGEMLGSVLDLSLV
                     SRVGRLLEDYTVTFRGFRPQIDLAATDLTAAVGEVVAAVTSPPYEEDGIIEARTEEEF
                     SRALAVRIA"
     gene            1492..>1975
                     /locus_tag="PKL26_07060"
     CDS             1492..>1975
                     /locus_tag="PKL26_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83489.1"
                     /translation="MQEPDDRQPIRFSAPVNDQWNYLLSTIWNQMMHLVVILDGRLDE
                     ERLRHAAELVMEAEPVLACRFVEAAVPYWEWIPSLRSESVFSIAGQGSICETSLKGAL
                     ARKYDPAAGPQAHFTLLRGDRDSLVISVNHAVCDARGILHISSLFARAYRALESNSGF
                     S"
BASE COUNT          382 a          623 c          569 g          401 t
ORIGIN      
        1 acgcgatcct gaccgcaagc gcccgggaaa actcctcctc tgtccgggcc tcgatgatgc
       61 catcctcctc gtacgggggg gatgtgacgg ccgccaccac ctccccaacg gcagctgtga
      121 ggtcggttgc ggcgagatcg atctgtggcc ggaagccccg gaacgtgacg gtgtagtcct
      181 cgaggagcct gcccacgcgg ctgacgaggg agagatcgag taccgacccg agcatctccc
      241 cccggtatac cttctcgagt ttctgggaga tctggttcgc cttgagcgcc aggtgtatct
      301 cgagcgcctg cccggagtgg aaatcaagag agaacctgtt gaaatcagag aggatctcat
      361 cgacaagatc gctgttgatc agcccccgga acctctccgc ctcccccgac tcttcgagct
      421 cgtcaagcac atgggccaca tacacgagcc tgccggcaat gatcgtgaag aggcgatcca
      481 cctcacccat cagctctccc gcatatgccg cggtatcctt cacccactca ggattcacga
      541 tctcgatccc ctcgaggcgc gaggcctcct tcttcattgc ctggagcgtg cgggtggttg
      601 ttgcggcatc cagcggacgt ccgtgccctg gaaggcagac ggtgattcca cccttctgca
      661 ggagcgcgat cacctggtcc agggtgaaga ggagagcttt taagtcccat cccttcagtc
      721 ctgcaactcc cggtgcggtg gagaagagga ggtctcccaa aaagagcgtc tcccccatac
      781 ggatgcagat gctgtccggg ctgtgaccag gtgcagggta gaactcgatc atcgtatctc
      841 cttccagcgg cagcgtctgc ctcgatatgg tgagtcctcc tcccagcgga agggtgtcca
      901 ggcaggtgat caccgaagac ccggcgatgt tgagtatttc atccctgtgt tctcccccgt
      961 ttccggatgg caggagcctg atatcgatcg ttatcgtccc cacctcctgg cgaatcaggt
     1021 ccgcgatggt ggccgcgcgg tcacggctct ccagcgcccg cgcacctgcc tcctggaccg
     1081 cgaccagcat gtggggaagc gtccggaacc ccgggtggtg caggagctgg tggcagtggt
     1141 cgaggtggac gtgcgtaagg cagatcaaca ccggtcgcgg cttttctgtg atcagctttc
     1201 gtatctctcc aagaagagta tctgcctgcc ccggaaggcc accggggtcg accaggacga
     1261 tgagtcgtgg agctgccacc aggtacgagt tcgaggatat gatgtccacc ttgcggagga
     1321 acggatagat cgaaacaccc tccacaccgg ggaggggctg ccatatctgg ctctccatat
     1381 cagccaattg caatcacctg cctcatccac tgcacacggc gaatccacgc gtgcgatcat
     1441 attctcttcc ccaggagctg atatctgata ccctgatatc aggagtgccc catgcaggag
     1501 ccagacgacc gccagcccat ccggttctcc gcgccggtga atgaccagtg gaactacctc
     1561 ctctcgacga tatggaacca gatgatgcac ctcgttgtca tacttgatgg ccggcttgac
     1621 gaagaaaggc tccgccatgc tgcggagctc gtaatggagg ctgagcctgt cctggcctgc
     1681 aggtttgtcg aggctgcggt gccttactgg gaatggattc cctctctccg gtcggaatcg
     1741 gtcttctcaa tcgccgggca gggaagcatc tgtgaaacat ctctaaaagg cgcgctcgcc
     1801 cgaaagtatg accctgcagc aggcccgcag gcacatttca cccttcttcg tggagacagg
     1861 gactcactgg tcatctctgt aaaccatgcc gtgtgcgacg cccgcggcat cctgcacata
     1921 tccagccttt ttgcccgcgc ctaccgggca cttgagagca attccgggtt ctccc
//
LOCUS       DAOQVJ010000335         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_74145_length_1970_cov_5.227676, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000335 DAOQVJ010000000
VERSION     DAOQVJ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74145_length_1970_cov_5.227676"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1970
                     /locus_tag="PKL26_07065"
     CDS             <1..>1970
                     /locus_tag="PKL26_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HNS83490.1"
                     /translation="DDTYIDKTTPRGALLSHYSDAYGSDAIMIIVESDDIMQVECLHY
                     IERLQADVANQQYVERVSGIVDLLKQGNGGTLPGSSAEINQIVGQAPPEVVDRYIPSK
                     MMTISVVSLQPGISSDRQEQALNNIRSIVSLSNPPPGVTVILSGSPAFSQEMQQEMGS
                     SMGILILAAMVLMVVAVMTLFNHVRYTLLPVIVVATGLILTFGFMGLAGIPISMVVIG
                     AFPVLIGIGIDYAIQIHSRLDEEVRKSSLPDAITTTITKTGPSVLIAMLATSMGFIAM
                     VFGPIPMVGDFGITCTIGVISCYLAALIIVPVFGILIKYRPRDPKGKINDVAACELGW
                     KGCDTTPVHQEGSRGSFIEKYDAFLGAVALKIAKNPVPILMLVFMVAIVGFNLDNDVP
                     ISADEKTFVPSEMPALQNMNKVTRAMGSTSTIPVIVTGEAVLNPDTLTWIRDFGEYEI
                     EHNDKITGVTSIATLLAQYNNGNLPTTEGEVQQVLDRIPAVTKDRYLNGKIETVLEFS
                     TVDMEISQARSLVQLIERDVAWNSPPPGVTARVTGQIEMFAALMDDISDSKLIMTVLG
                     FAFILAFLLVVYRRVHAVSPLLPIVAIVGWNGAIMYLLGLDYTPLTAVLGSMTIGVAS
                     EYTVLIMERVDEELARGLDLFSAIQTSVQKIGA"
BASE COUNT          407 a          573 c          553 g          437 t
ORIGIN      
        1 gatgatacct atattgacaa aacgactccc cgcggggccc tcctttccca ctattccgat
       61 gcatacgggt ctgacgccat catgatcatc gtcgagtccg acgatatcat gcaggttgaa
      121 tgcctgcact acatcgagcg gctccaggct gatgtcgcaa accagcaata tgtggagcgg
      181 gtgtccggca tagtcgatct cctgaagcag gggaacggcg ggacgctccc gggatcatcc
      241 gctgagatca accagatcgt cggccaggcc ccaccggaag tcgttgatcg ctacatccct
      301 tcgaagatga tgacgatcag cgtcgtttcg ctccagccgg gcatctcgag cgacaggcag
      361 gagcaggccc tcaacaacat ccggagcatc gtctcgctct cgaaccctcc tcccggtgtc
      421 accgtcatac tctcgggatc tcctgctttc tcccaggaga tgcagcagga gatggggtcg
      481 tcgatgggaa tcctcatcct cgccgccatg gtgctgatgg tcgtagcggt gatgaccctc
      541 ttcaaccacg tgcgctatac tttgttgccg gttattgtcg ttgcaacggg tctcatcctg
      601 acgttcgggt tcatgggtct ggcaggaatt cccatcagca tggtggtgat tggggcgttt
      661 cctgtgctca tcgggatagg catcgattac gcaatccaga tccactcgag gcttgacgag
      721 gaggtccgga aatcttctct tcctgacgcg atcaccacca cgatcacgaa gaccgggccg
      781 tctgtgctga tcgccatgct cgcaacttcg atggggttca ttgcgatggt ctttggtccc
      841 atccccatgg tgggtgactt cgggatcacc tgtaccatcg gggtgatatc gtgctacctt
      901 gccgccctca tcatcgtccc ggtctttggc atcctgataa agtatcgccc aagggatcca
      961 aaagggaaga tcaacgatgt cgccgcctgc gaactcgggt ggaagggctg cgatacgacc
     1021 cctgtccacc aggagggttc acgcgggtcg ttcatagaaa aatatgatgc gttcctggga
     1081 gcagtcgcgt taaagattgc gaagaacccg gtgcctatcc tcatgctcgt cttcatggtg
     1141 gcaatcgtcg ggttcaacct ggataacgat gtccccatca gtgccgatga gaagactttt
     1201 gttccgtccg agatgcctgc gctccagaac atgaacaagg tcacgagggc tatgggctcg
     1261 accagcacca tccctgtcat tgtgacggga gaagcggtcc tgaaccccga tacgcttaca
     1321 tggatacggg acttcgggga gtacgagatc gagcacaacg acaagataac cggcgtgacc
     1381 agtatcgcca ccctccttgc gcagtacaac aacggaaacc tccccacgac cgaaggcgag
     1441 gtccagcagg tgcttgaccg cattcccgcc gtcacaaaag accggtacct gaacgggaag
     1501 atcgagacgg tgctcgagtt ctcaactgtc gacatggaga tatcccaggc ccgatcactg
     1561 gtccagttga tcgagaggga cgttgcgtgg aactccccgc cgcccggggt gaccgcccgt
     1621 gtcaccggcc agatcgagat gtttgctgcg ctgatggacg atatcagtga ttcgaagctg
     1681 atcatgactg tgctgggctt tgcattcatc ctcgcctttc tcctcgtggt atacaggcgg
     1741 gttcatgcag tctcgccgct tcttcccatc gtggcaatcg tagggtggaa tggtgcgatc
     1801 atgtatctcc tgggcctcga ctacacccca cttaccgccg tgctcggttc gatgacgatt
     1861 ggagttgcct ccgaatatac tgtcctgatc atggagaggg tcgacgagga actggcacgg
     1921 ggcctcgatc tcttttcggc aatccagacc agcgtgcaga agatcggcgc
//
LOCUS       DAOQVJ010000336         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_74152_length_1970_cov_3.601044, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000336 DAOQVJ010000000
VERSION     DAOQVJ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74152_length_1970_cov_3.601044"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..367)
                     /locus_tag="PKL26_07070"
     CDS             complement(56..367)
                     /locus_tag="PKL26_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83491.1"
                     /translation="MEARHLNDRIIRTFAIAGASEKGFTITQSFLKAAEGIETMHDIV
                     TTRKMENGVACYYGESGKEKFESFTYRELIDMKINALDLLDDPRNYAVDTEKHTLVMK
                     K"
     gene            complement(345..1820)
                     /gene="pap"
                     /locus_tag="PKL26_07075"
     CDS             complement(345..1820)
                     /gene="pap"
                     /locus_tag="PKL26_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNS83492.1"
                     /translation="MLEKIDLGKHIDDHTYAKKIAPLYQRLGVLQRAHWDLRIPIVIV
                     VEGWNASGITTVISELIQYLDPRGFVLHSIGSPKDEERARPLLWRFWTRTPARGRMAI
                     FARSWYSRSLAEQVSGIDWKSRIKRAIFDINTFERQLTDDGVVIIKFFLHISKEEQRK
                     RLNEREKDALTSWMITQGDWDFHAHYDDYLPIIEDYLSGSDTRHSPWTVIGATDGNYT
                     RFKVYSTVIRTLEEKQKILEKKRQEKCGPALAKPPRNKIKRSAEAPRQYTRAEYEIIL
                     QECQEQVHDIQYLLFKRRIPLIIVYEGWDAAGKGGNIMRLVRAMNPRGYDVVPIARPD
                     QTELEHHYLWRFYRRFPAAGHITIFDRSWYGRVLVERIEGFCTESEWKRAYHEINEME
                     EAYVEGGGGLIKFWLEVSREEQYRRFIARQNDVLKEWKMTEEDWRNREKWKEYENAVD
                     EMLARTSTSIAPWTVIESDDKYYARLKAIQTVISYGSEALE"
BASE COUNT          397 a          558 c          477 g          538 t
ORIGIN      
        1 tatcctcatt ctctagtcgc catgcagagg ggagaaagag aagtgtggat cttacctact
       61 tcttcatcac gagtgtatgc ttttccgtgt cgactgcgta attccttgga tcatccagaa
      121 ggtctagcgc gttgatcttc atgtcaataa gctccctata ggtgaagctc tcgaatttct
      181 ctttcccgct ctcaccatag tagcaggcga caccattctc cattttcctg gtagtgacaa
      241 tatcatgcat agtctcaatt ccttcagccg cctttaagaa actttgtgtt atggtaaacc
      301 ccttttccga tgctcctgct atcgcgaaag tgcgaatgat acgatcattc aagtgcctcg
      361 cttccatacg aaatgaccgt ttgaatcgct ttcagccttg catagtactt gtcgtctgac
      421 tcgatcacgg tccatggtgc gatactggtg ctggtgcggg caagcatctc gtcgaccgca
      481 ttctcgtatt ctttccattt ctcccggttg cgccagtcct cctcggtcat cttccattcc
      541 ttgagaacgt cgttctgcct ggcgatgaac cgcctgtact gctcttccct gctcacctcg
      601 agccagaact taatcaaccc tcccccacct tccacgtacg cctcctccat ctcgttgatc
      661 tcgtggtagg cacgtttcca ctcactctct gtgcagaacc cctctatcct ctccaccagt
      721 acccttccgt accaactccg gtcgaagatg gtgatatgac ctgctgcggg gaacctccgg
      781 tagaacctcc agaggtaatg gtgctcgagc tcggtctggt ccgggcgggc gatgggaacc
      841 acatcatagc cgcgggggtt catcgcgcgt accagccgca tgatgtttcc ccccttcccc
      901 gcggcgtccc acccttcata tacgatgata aggggaatcc tgcgcttgaa gagcaggtac
      961 tggatatcat ggacctgttc ctggcactcc tggaggataa tttcatattc tgctctggta
     1021 tactgccttg gtgcttctgc gctccgtttt attttgttcc tggggggttt tgccagcgca
     1081 ggtccacatt tttcctgccg tttcttctcc aggatctttt gtttttcttc aagggttctg
     1141 atcactgttg aatatacctt aaaccgcgtg taattgccat ccgttgcacc tatcacagtc
     1201 catggtgagt ggcgggtatc ggaaccagaa aggtagtcct caataatcgg gagatagtca
     1261 tcatagtgcg catgaaagtc ccagtcgccc tgggtgatca tccaagaggt gagtgcatcc
     1321 ttctccctct cgttcagccg ctttctctgc tcttccttgc tgatatggag gaagaacttg
     1381 atgatgacaa caccatcgtc ggtaagctgc cgctcaaagg tgttgatatc aaagatggcg
     1441 cgttttattc gggatttcca gtcaataccc gatacctgct cggcaagcga ccgggaatac
     1501 cagctccggg cgaaaatggc catccttccc cttgcaggag ttctcgtcca gaacctccac
     1561 aggagggggc gtgcacgctc ttcatctttt ggtgacccga tggaatggag cacgaatcca
     1621 cggggatcga ggtactggat cagctcactg atcaccgtgg tgatgcccga cgcattccag
     1681 ccctcgacaa cgatcacgat gggaatcctg agatcccagt gtgcccgctg gaggacaccg
     1741 agtctctggt acaggggggc gatcttcttt gcgtaggtgt gatcatcgat atgcttccca
     1801 aggtcgatct tctcaagcat gccgtctccc gctgaatgaa tgggactgtt gcattaggaa
     1861 gcattaacat tgggatttac atccgggagg ggaggaccgg ggatcactcg ccgcactgct
     1921 gcacggcatt aaaacttcca ggtgattcag gctgccttcc agggcttttc
//
LOCUS       DAOQVJ010000337         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_74508_length_1964_cov_4.223153, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000337 DAOQVJ010000000
VERSION     DAOQVJ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74508_length_1964_cov_4.223153"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKL26_07080"
     CDS             complement(<1..406)
                     /locus_tag="PKL26_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNS83493.1"
                     /translation="MRHIISIRDLDRAEINGILDHAQALQEQDYNSQALTGKLLGLLF
                     FEPSTRTRMSFASAVARLGGHSISMDSVEASSMVKGETLADTIRVVSGYVDAIVLRHP
                     KEGAARLASEFAQVPVINAGDGAGQHPSQTLLD"
     gene            complement(709..>1964)
                     /locus_tag="PKL26_07085"
     CDS             complement(709..>1964)
                     /locus_tag="PKL26_07085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS83494.1"
                     /translation="VHAKTGKAWNDPSVLQKIRRAIVSQKDEYWREGEKKRVMYQKGY
                     DVLGYLAYQAPVYFIQAMHLLYDLALAGCLKQHMRVLDLGCGPGVVPLAISSFLERIG
                     GCSAEIYSVEKSEEFIEAFRFLADHVPTPRRSVALHPPLNADLVTVPLSCLPPSLDLI
                     VLQNVLNEMSGHSPRDKGRLVCRFAEALSPEGSILLVEPADMVNSMDLRVVVHHATGS
                     GLFLHAPCRFLWNTQCTPDRCWSFVEKPPIHATGLMEALASGEEGYRYCNTDIKYSFA
                     LLRKVPPNPVPGLPLSPRNYARLSALHRHINRRINVAGVVMSGNLGDHKTYVIKICDG
                     TTKKPVYAILPSYHLSHENSALLSIPYGSIAEFTSVLARFNEKHHAYNLLVSRESRVK
                     EFPAQSRAADWNLTGPREKRNKLKK"
BASE COUNT          414 a          512 c          593 g          445 t
ORIGIN      
        1 ggtcaaggag cgtctgcgaa ggatgctggc cggccccgtc gcctgcattg atgacgggga
       61 cctgcgcgaa ctcacttgcc aggcgtgctg caccctcctt gggatggcga aggacgatgg
      121 cgtcgacgta tccgctcaca acccgtatgg tgtctgcaag ggtctcgccc ttcaccatgg
      181 agcttgcctc aacgctgtcc atcgagatcg aatgcccgcc cagacgggcc acggcagacg
      241 caaacgacat ccttgtccgg gtgcttggtt caaagaagag gagaccaaga agcttccctg
      301 tcagggcctg ggaattgtaa tcctgttcct ggagggcctg tgcatggtcc agtattccat
      361 tgatctcggc cctgtccaga tcacggatgg atatgatgtg ccgcatcgtt tttgccctct
      421 ctctctggca gattctgtcg ggattgcgat cgattctgcc tgtcgtgcac ctgtcaatgt
      481 tgtatgggcg ggccaagcca taaatacatg tcaaagggac gaagaaatgg aaattttcca
      541 tgaagcatcg ggtgaaggac ttcagggtga aaaagtcact cttctattca cgataaggaa
      601 aaggagttcc gcagaagaac gggccatgac agtgcccggg tgatcggcgt gcccggctgg
      661 aatccctatt caagcggccc ttgcatggag agccatactg agaagggatc attttttcag
      721 tttattcctt ttttcacggg gacctgtgag attccagtct gcggctctac tttgtgcggg
      781 aaactccttt actctgctct ccctgctcac aaggaggtta tatgcatggt gtttctcgtt
      841 gaacctggca aggacggatg taaattcagc aatggagccg tagggaatcg acaggagcgc
      901 gctgttttcg tgagagagat ggtaggatgg caggattgca tagaccggtt ttttcgtcgt
      961 cccatcgcat atcttgatga cgtaggtctt gtggtcgccg aggttgccgg acatcaccac
     1021 gcctgcaacg ttgatcctcc tgttgatgtg ccggtgcagg gcagagagcc gcgcatagtt
     1081 acgcggcgag agagggaggc ccgggacagg gtttggggga accttgcgga gcagggcgaa
     1141 cgagtacttg atatcggtgt tacagtacct gtacccctct tcccccgatg cgagcgcttc
     1201 catgagtcct gtcgcgtgga ttggcggctt ctccacgaaa ctccagcacc ggtctggtgt
     1261 gcactgggtg ttccagagga acctgcacgg cgcatggagg aagagtccgg atccggttgc
     1321 atggtggacc acgacccgga gatccatcga gttgaccatg tctgcaggct caacgaggag
     1381 aatgctccct tcgggagaga gggcttctgc gaaccgacat acaagtctgc ccttgtcgcg
     1441 tggcgagtgc cctgacatct cgtttaaaac attctggagg acaatcaggt caagcgaggg
     1501 aggaaggcag gagagaggga ccgtgacaag gtcggcgttg agagggggat ggagagcgac
     1561 tgaccgcctt ggggtgggga cgtggtcggc caggaaacga aaagcctcga tgaactcctc
     1621 cgacttctcg accgagtaga tctcggcaga acatccgccg atcctttcta gaaaactgct
     1681 gatcgcaagc gggaccactc cggggccaca accaaggtca agcactctca tgtgttgctt
     1741 caggcaacct gccagtgcaa ggtcgtacag gaggtgcatg gcctggataa agtatactgg
     1801 cgcctggtag gccaggtacc cgagtacatc gtaccctttc tgatacatta ccctcttttt
     1861 ctctccctcg cgccagtatt cgtccttctg ggacacgatt gcgcgccgga tcttctggag
     1921 gacagaaggg tcgttccagg ctttgcccgt ctttgcatgg acat
//
LOCUS       DAOQVJ010000338         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_74813_length_1959_cov_3.789391, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000338 DAOQVJ010000000
VERSION     DAOQVJ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74813_length_1959_cov_3.789391"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKL26_07090"
     CDS             complement(<1..458)
                     /locus_tag="PKL26_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS83495.1"
                     /translation="MREEASTVKAQRSSPSFAKHHPMAGQVILVTGSSDGIGKAAARE
                     LAARDAEVIVHGRDPGKGRQVIRELKSQVWNRNLDLVTADFTSLEQVRRMAAEVSSRY
                     TCLDVLVNNAGTYEKQRSLTPDGIETTFAVNSLAPFLLTHELLPLLRKGHL"
     gene            complement(434..1912)
                     /locus_tag="PKL26_07095"
     CDS             complement(434..1912)
                     /locus_tag="PKL26_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNS83496.1"
                     /translation="MKDRRRIDLKAIAREAMEKYGFEPEFPKTVIKEVNAMLPGLSAP
                     GRHDIRDLRSLLWSSIDNYDSEDLDQLEYCEAGTRGEILVRVAIADVDHFVTKGSHTD
                     RHAAHNGTSVYLGVETFPMLPDRLSKGISSLLPGEDRCAMVVEYKVLPGGSVRNGGIY
                     RALVNNKAKLVYEEVGDWLEGVAPVPVSVSSVTGLEKQIRLQAEVTRRLRSRRMQKGA
                     LDLETLEAEPVMKGETVHSLVIQEQNAARQIIEEFMVAANGTMVHVLGGAKVPMIQRV
                     VRVPKHWDGIMETAAAYRYKLPKQPDSKALAKFLDRQRAADPVRFPDLSLTIVKLMGP
                     GEYVPFVPGDTPIGHFALAVVDYTHSTAPNRRYVDIVNQRLLKAVLDGEAVPYSGHEL
                     GRLAEWLSDREKASQKAERFMRKVAAALLLERRIGETFDAIVTGAAEKGTYVRLLDPP
                     AEGRVVEGERGLRVGNKVTVRLLSTDPPRGYVDFACVKKPPR"
BASE COUNT          355 a          604 c          567 g          433 t
ORIGIN      
        1 agatggcctt tccggagcag gggaagaagt tcatgcgtga ggaggaacgg agcgagagag
       61 ttcacggcaa acgtggtctc gatgccgtcc ggagtcaggc tcctctgttt ctcgtaggtc
      121 ccggcgttgt tcaccagaac gtccaggcag gtataccgcg acgagacctc ggctgccatt
      181 ctcctcacct gctcaagtga agtaaaatcc gcggtgacga ggtcgaggtt ccggttccag
      241 acctgacttt tcagttccct gatcacctgc ctccccttcc cggggtccct cccgtggacg
      301 atcacttcgg catcgcgtgc cgccagctca cgggcagcag ccttccctat cccatccgag
      361 gaaccggtta cgaggatgac ctggcctgcc atggggtggt gtttcgcgaa ggatggcgat
      421 gaacgttgtg cctttaccgt ggaggcttct tcacgcatgc aaaatccaca tatccccgtg
      481 gaggatcggt cgagagcagc ctgactgtga ccttgttgcc cacccggagc ccgcgttccc
      541 cttccaccac tcttccctcc gcggggggat cgagaagacg cacgtatgtc cctttttctg
      601 ctgcacccgt cacaatcgca tcaaaagtct cgccgatacg tctttcgagg agtaaggcgg
      661 ctgcaacctt cctcatgaac cgctctgcct tctgggaggc cttctcgcgg tccgagagcc
      721 actctgcgag ccggccaagt tcgtggcccg agtagggcac ggcctcgccg tcaagcaccg
      781 ctttcaggag gcgctggttt acgatatcca cgtagcggcg gttcggggcg gtggagtgcg
      841 tgtaatccac cactgccagc gcaaagtgtc cgatcggtgt atcgccggga acaaagggga
      901 catattcacc cggccccatc agcttcacga tcgtcaggga gaggtcgggg aaccgcacgg
      961 gatctgccgc cctctgcctg tcgaggaact ttgcgagcgc ttttgagtcc ggctgttttg
     1021 gcaatttgta ccggtatgct gcagcagtct ccatgatccc gtcccagtgt tttgggactc
     1081 tgaccacccg ctggatcatg gggactttcg cacccccgag gacatgcacc atagtgccgt
     1141 ttgcagccac catgaactcc tcgattatct ggcgggctgc attctgctcc tggatcacga
     1201 gggaatgcac cgtttcaccc ttcatgaccg gctcagcctc cagggtctcc aggtcaaggg
     1261 cacccttctg catgcggcgg ctcctcaacc ggcgggtcac ctccgcctga agccggatct
     1321 gcttttcgag tcccgttacg ctgctgacag agacgggaac tggggcgaca ccctcgagcc
     1381 agtcgccgac ctcctcgtac acgagctttg ccttgttgtt gaccagcgcg cggtagatac
     1441 ccccgttccg gacgctccct cccggaagga ccttatactc caccaccatc gcgcaccggt
     1501 cctctccggg aagaagagac gagatcccct tcgagagccg gtcgggaagc atcgggaacg
     1561 tctcgactcc caggtatacg gacgtgccgt tgtgggcagc gtgccggtcg gtatgcgagc
     1621 cctttgtgac aaagtggtcc acgtccgcga tggcgaccct gaccaggatc tctcccctcg
     1681 tgccggcttc gcagtactcc agctggtcga ggtcttctga atcgtaattg tcgatggaag
     1741 accagaggag ggatctcaag tccctgatat cgtgccgccc tggtgcggag aggccaggta
     1801 acatggcatt gacctctttt atcactgttt tggggaattc cggttcgaat ccatactttt
     1861 ccatcgcctc gcgggcgatg gccttcaggt caatgcggcg ccggtctttc atgcaggatt
     1921 gagagtggtt tgtttcaatg cttaaatagt gtgggaaag
//
LOCUS       DAOQVJ010000339         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_75188_length_1953_cov_3.650158, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000339 DAOQVJ010000000
VERSION     DAOQVJ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75188_length_1953_cov_3.650158"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1679)
                     /locus_tag="PKL26_07100"
     CDS             complement(201..1679)
                     /locus_tag="PKL26_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNS83497.1"
                     /translation="MKRLETLAVIAHDMGIIFKFLGVITLVPFVVLFIFEEWEMVVPL
                     ASVPLTFLLLGLLISRIPRREYVPQISVALAAVALIWLAIAVVGALPFVIGVHMSWTD
                     SIFEAMSGWTGTGFTVIGTLDSTPKTLLFWRSFMQWVGGVGVIAFGIAMLSRSSLMQH
                     QLYRSEGRSEELMPSVVSTGRRMWVIYLFLTVVFTGMVMLSGVPLWDALNLTMVSIAT
                     GGFSLHDAGMAYYHNVVLEGLLIPVMIAGALPFKLYFLMYRGKLRPFFRDRVVHLILA
                     LALVGSAVVTLDLHIFNNFPLETAVRQGFFCTISGLTCTGLQNSDLHWVAGPLIVITI
                     LMMIGGAAGSTSGGIKANRVILGYEGLVWWFRRFFARGKEMVPFRHEGKVIPTRISEV
                     ELSKNMLIIVLYVLIIFVATVIGLHLAATDFKVHEVVFEEVSTMSNVGLGLGYLIPTS
                     PLSTKWIFIFLMWIGRLEIIPVLILAMGLARGFEARVKGSRR"
BASE COUNT          484 a          556 c          531 g          382 t
ORIGIN      
        1 gttctcctca tcatcaccgc gatcgtcggg ttctggaagg gaaaagtggt ctgaaggtcc
       61 atgcctctct ctggagtgag gagaaccggc actgatcgct ccggaatccc ccttaaaaaa
      121 aatctggtca ttcccttgtt ccccccgaga tcctgcagcc acgaacctgc aaagaaagga
      181 ttttttttca caaaaaccac tcatctcctg ctccccttca ccctggcttc gaaccccctg
      241 gcgagtccca tggcaaggat aaggacaggg atgatctcca gtcttccaat ccacatcagg
      301 aatataaata tccacttggt cgagagcggg cttgtgggaa tcaggtaccc gaggccgagc
      361 cccacgttgc tcatcgtgga gacctcctcg aacaccacct cgtggacctt gaaatcggtt
      421 gccgccaggt ggaggccgat gacagtagcc acgaagatga tcaggacata gaggacgatg
      481 atcagcatgt tcttggagag ttcgacctcc gagatccgcg tggggatgac ttttccttca
      541 tgccggaacg ggaccatctc ctttcccctc gcaaagaagc gccggaacca ccacacgagg
      601 ccttcgtacc caaggatgac ccggttggcc ttgatgcccc cggaagtgct cccggccgcc
      661 ccgccgatca tcatcagaat tgtgatgacg atgaggggtc ctgcgaccca gtgaaggtcc
      721 gagttctgga gaccggtaca ggtcaacccg gatatcgtgc agaagaagcc ctgccggacc
      781 gcggtctcaa gggggaaatt attgaagata tggagatcga gagtgaccac ggccgatccc
      841 acgagcgcga gtgccaggat aaggtggacg accctgtccc ggaaaaacgg ccttagcttc
      901 cccctgtaca tgaggaaata cagcttgaac ggcaacgcac ccgcgatcat gaccgggatg
      961 agaagtccct cgaggaccac gttgtgatag tatgccatcc cggcatcatg gagtgaaaat
     1021 ccgccggtgg caatgctgac catcgtcagg ttgagggcgt cccagagggg gacccccgag
     1081 agcatcacca tgccagtgaa gacaaccgtg aggaagaggt agatcaccca catccgcctt
     1141 cccgtggaga ccacgctcgg catcagctcc tcggaccgcc cctcggaccg gtagagctgg
     1201 tgctgcatga ggcttgacct ggagagcatg gcgatgccaa acgcgatcac cccaaccccg
     1261 ccgacccatt gcatgaacga gcgccagaag aggagggtct ttggggtgga gtcgagtgtc
     1321 ccgatcaccg tgaacccggt tcctgtccac cccgacatgg cctcgaagat gctgtccgtc
     1381 cagctcatgt ggacaccgat cacaaacggg agagcgccca ctactgcaat ggcaagccag
     1441 atgagtgcca ctgcagcaag ggcgaccgag atctgcggaa catactccct tcttggaatg
     1501 cgggatatga gaagaccgag gagcaaaaat gtcagcggaa cggaggccag cggcaccacc
     1561 atctcccatt cttcaaatat aaatagcaca acgaagggaa cgagagtgat cactccaagg
     1621 aacttgaaga tgatccccat gtcatgggcg atgacggcca gtgtctccag ccgcttcatg
     1681 atgagatgat tatcaggggt agtttatctt cattctgtat gatcgatagg gcaaaatcaa
     1741 catggtctcc gttgattacg gcgggatctt ccggaatccg tgcatcactc gcatcatttc
     1801 ggttgatgca ccgcagacac tctctcccaa cgtgttccct gccttatcat cgtactggag
     1861 aatggtatga tgttttaaag gagagcattt cttcaaaaaa aaggaatgcg agggatggga
     1921 tttgaaccca agaactcctg cgagactaga ccc
//
LOCUS       DAOQVJ010000340         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_75511_length_1948_cov_4.186476, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000340 DAOQVJ010000000
VERSION     DAOQVJ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75511_length_1948_cov_4.186476"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKL26_07105"
     CDS             <1..784
                     /locus_tag="PKL26_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS83498.1"
                     /translation="SIGISVRTLIDLRQLKSDIGLLVVSSAVIDDVIGIMMLGILAAL
                     VTGEGYGAGVLTTIVLAILFLVVVLTAGKRVLVWVFQESHKAHTHEMPYSVALICGLL
                     CALAANEAGLHFAIGAFIAGLILGDSIRSDRSLYDSLSDLAFGFFVTLFFAYIGLLFP
                     PSLAGVPLVFFAVLVAVSFASKIVGGFIGSFRTLQNPRKALVVGFGLVPRGEVALVVA
                     KVSLTAGIISISLFSAVTLMVVITVFAAPFLMVRGFTWLRGD"
     gene            complement(781..1290)
                     /locus_tag="PKL26_07110"
     CDS             complement(781..1290)
                     /locus_tag="PKL26_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS83499.1"
                     /translation="MLERDTHPADEAAGNQQKSAGKDIDIIAVSDLMTRDFEKILPDT
                     PIDHCITHFSQRGCRDLVVVDHEGRFLGIVTPVDLLTHLSPAIGVRSRKKSGCIECII
                     VGNASTAEDVMARKHITVRGDTPVSEALRLLEKYHHPDLVVVDQEGVAMGVVEICTII
                     AHLKVTGKV"
     gene            complement(1404..1925)
                     /locus_tag="PKL26_07115"
     CDS             complement(1404..1925)
                     /locus_tag="PKL26_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M54"
                     /protein_id="HNS83500.1"
                     /translation="MELLIFWDARAPAGIQSAFSREIERLFPLKTKICESPFVVTGYI
                     PSRRQTNATSLLDSLDLYRKRSGVSGLVLLVVSEDLCRPGDEYLFGLSRRSTGNAVVS
                     TARLGNEFYGYPPDDGDLIDRLSKESAHEAGHLLGLAHCADPECIMHNPLLLEDITRQ
                     KRAFCAACRAQIP"
BASE COUNT          382 a          548 c          551 g          467 t
ORIGIN      
        1 ttccattggg atctcggtcc gcacgctcat agacctgcgg cagctcaaat ccgatatcgg
       61 gctcctggtg gtcagttccg cggtaatcga cgacgtgatt ggcatcatga tgctcgggat
      121 actcgcagca cttgtcacag gagaaggcta tggtgcgggt gttctcacca cgattgttct
      181 cgccatactc ttcctggtgg tggtgctgac tgcaggaaag cgggtgctcg tgtgggtatt
      241 ccaggaatcc cacaaggcgc atacccacga gatgccctat tccgtcgccc tcatatgcgg
      301 tctcctctgt gcacttgcag cgaacgaggc agggctccat ttcgcgatcg gcgcgttcat
      361 cgcagggctc atcctcggcg atagcatccg gagcgaccgg tcgctttacg acagcctctc
      421 cgatcttgcg ttcggcttct ttgtcacgct cttctttgca tatattgggc tcctctttcc
      481 gccgtccctt gcaggagttc cactggtatt ctttgcggtg ctcgtggcag tgtcatttgc
      541 aagcaagata gtcggaggat tcatcgggtc ttttagaacc ctccaaaacc ccaggaaagc
      601 actcgtagta ggattcgggc ttgttcccag gggtgaggtg gcgctcgtag tcgccaaagt
      661 ctcgctgaca gcggggatta tctctatttc cctcttctcg gctgtaaccc ttatggtggt
      721 catcacggtc tttgccgcgc cattcctgat ggtccggggt tttacctggc tccgtggaga
      781 ttaaaccttc ccggtcacct tgagatgtgc gatgattgtg cagatctcga cgacccccat
      841 cgcaactccc tcctgatcga ccaccacgag gtcagggtga tgatacttct caagcagcct
      901 gagcgcctcg gagaccggag tatccccgcg aacagtgata tgcttcctcg ccatgacgtc
      961 ctctgcagtc gatgcattgc ccacgataat gcactcgatg caccctgact tcttcctgct
     1021 ccggacacct atggcgggac tgagatgggt aaggagatca acgggggtga cgatccccag
     1081 aaaacggcct tcgtggtcga caaccacgag gtccctgcag cccctttgcg agaaatgcgt
     1141 gatgcagtgg tcgatcgggg tatcgggaag gatcttttcg aagtcgcggg tcatgagatc
     1201 tgagacggct atgatatcga tgtcttttcc tgcacttttc tgctggttac ccgcagcctc
     1261 atcagccgga tgcgtatccc tctccaacat aaaaggtcct taattccttc gcttcgggag
     1321 ttgatggtgc ctgcgttcat ataaagggag tggctcccaa aaaataaaag caaggaatca
     1381 ggcctatccc cctccagttg gggttacggg atctgtgccc ggcatgctgc acagaatgcc
     1441 cgtttctgcc ttgtgatgtc ctccaggaga agagggttgt gcatgatgca ttccgggtct
     1501 gcgcaatgcg caagtcccag gaggtggcct gcctcgtggg cggactcctt tgacaggcgg
     1561 tcaatgagat ccccgtcgtc aggcggatac ccgtagaact cgtttcccag ccgggcagtg
     1621 gataccacgg cgttccccgt cgaccgccgg gagaggccga agaggtattc gtcccctggc
     1681 ctgcagaggt cctctgatac cacgaggagg accaggccgc tgacacccga gcgcttcctg
     1741 tagaggtcaa gagagtcaag gagcgatgtg gcattggtct ggcgccttga agggatgtag
     1801 ccggttacca caaaagggct ctcgcatatc ttcgtcttga gggggaagag gcgttcaatc
     1861 tccctcgaaa atgcggactg gatgccggct ggagcccgtg catcccaaaa aataagcagt
     1921 tccatctctc aatataagtg gatatggc
//
LOCUS       DAOQVJ010000341         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_75581_length_1947_cov_3.617336, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000341 DAOQVJ010000000
VERSION     DAOQVJ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75581_length_1947_cov_3.617336"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKL26_07120"
     CDS             <1..239
                     /locus_tag="PKL26_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83501.1"
                     /translation="GAVLDGRVHLIAHQGVRTAQSGRPGGIRSVTGTMVYIHRMTGTT
                     PVTLRVKPARIMSLGLLCPVPYVMVLAAAAARTS"
     gene            complement(352..849)
                     /locus_tag="PKL26_07125"
     CDS             complement(352..849)
                     /locus_tag="PKL26_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS83502.1"
                     /translation="MKIGIIGDTHDHLPNIGRAVEALIQEEVDMVLHTGDYIAPFVIP
                     LLGRIPVRVVGVFGNNDGDRELLATRSREQQNIELSGFFADIRVDGERVALMHGHDRA
                     LLAECIGSAKYDIVVSGHTHQCSIYREERTLGINPGEICGYLTGNPTYAIYETGERVA
                     RLLPL"
     gene            927..1292
                     /locus_tag="PKL26_07130"
     CDS             927..1292
                     /locus_tag="PKL26_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83503.1"
                     /translation="MTGREDEREIVCGLERAIVGNDRIARRRLLDRLVVLLFTTDTAA
                     PALVSHGGIRVLLTAARDRDEKIRLHAVHALSRLADSGFASELRDAGARTSIAPLMED
                     PYLPLRAMAEKLLEKIDGS"
     gene            1443..>1947
                     /locus_tag="PKL26_07135"
     CDS             1443..>1947
                     /locus_tag="PKL26_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS83504.1"
                     /translation="MKMRIGGVEVAGADERWMPVVNPATGEEIDRVPEGTLYEVDDAV
                     KAAGDAFSSWAGRAPRERGLLLFRGAALVRERHENLARLLTMEQGKPLKDAVDEVRGF
                     ANVLEYYAALSGSLAGEYIHLGKAGDAVVAREPFGVCGAIVPWNMPALLMAWKVAPAL
                     VAGNTMVF"
BASE COUNT          409 a          569 c          589 g          380 t
ORIGIN      
        1 agggtgctgt tctcgatgga agagttcatc tcatcgctca ccagggagtg agaacagccc
       61 agtcagggag gccgggcggg atacgaagcg ttacaggcac gatggtctac atccacagga
      121 tgaccgggac aacacctgtg acattgaggg taaaacctgc acggatcatg tcccttggtc
      181 ttttgtgccc ggtcccgtat gtgatggtac tggctgcggc ggctgcccgc acttcgtgag
      241 ggagaccccc tcctgggagg acgaggacaa atactccaat tccggccagc aatccgatta
      301 ggcggcgcat gtgagagaga tgttctggcc aatcgccctt cgaacctggg atcagagagg
      361 gagtaaccgg gcaaccctct cgccggtctc gtagatggcg tatgtcggat tcccggtaag
      421 atagccgcat atctcgccag gattgattcc aagcgtgcgt tcctcccggt atatcgagca
      481 ctggtgagtg tggcctgaaa cgacgatgtc atactttgcg ctcccgatgc actcggcaag
      541 gagcgcccgg tcgtggccgt gcatcagcgc aacacgctcg ccatccaccc ggatgtcggc
      601 aaaaaatccc gacaactcga tattctgctg ctcgcgggaa cgcgtcgcca gcagttcgcg
      661 atccccgtcg ttgttcccga ataccccaac cacccggacc gggatccttc ccaggagagg
      721 gataacgaat ggcgcgatgt aatccccggt atggagcacc atgtcaacct cctcctggat
      781 aagcgcctcc accgcgcgcc cgatgttggg gaggtgatcg tgggtgtccc cgatgatgcc
      841 aatcttcatc tcatgcctca catcctcaat attggcagga ctacataaag gaagcattat
      901 tggtgcaaaa aagccatctg gagaggatga ccgggcgcga ggacgagagg gagatcgtat
      961 gcggacttga gcgggcgatt gtcggcaatg accgcattgc acgacgtagg ctccttgacc
     1021 gcctggtggt gctcctcttt accaccgata ccgccgcacc agcgctcgtc tcccatggtg
     1081 gaatccgcgt cctcctgacc gctgcaagag acagggacga gaagatccgc ctgcacgccg
     1141 tccatgctct ttccaggctc gcagattccg gctttgcgtc agagttgaga gatgccggtg
     1201 caaggacttc catcgcgccc ctcatggaag acccgtacct tcccctgcgg gccatggcag
     1261 agaagcttct tgaaaaaatt gacggttcct gacataccga ctgtgccagc gacaggcgcc
     1321 ggccaatgac accacatggc tttttacacc cgcttccggc gcgaatgctt ctttagggtt
     1381 ccactccgca cgccacgggc cagtgtgaac caataatatg gatcggccat aatccttcgt
     1441 ttatgaagat gaggattggc ggcgtggagg tggcaggagc cgacgaacgc tggatgccgg
     1501 tcgtcaaccc ggcgaccgga gaggagatcg accgcgtacc ggaaggaacc ctctacgaag
     1561 tggacgatgc ggtaaaagcg gcaggagacg cgttctcttc ctgggccgga cgggctccga
     1621 gggagagggg cctccttctc ttcaggggtg cggcactcgt ccgggaacgg cacgaaaatc
     1681 tcgccaggct tctcaccatg gagcagggaa agcccctgaa ggacgcggtt gacgaggtca
     1741 ggggttttgc caatgtcctc gagtactatg ccgcgctctc cggcagcctc gcaggagagt
     1801 atatccacct agggaaagcg ggcgatgccg tggtggcccg ggagcccttt ggggtctgcg
     1861 gggcgattgt cccatggaac atgcccgcac ttcttatggc ctggaaagtg gcgcccgccc
     1921 tcgtggcagg aaacaccatg gtattca
//
LOCUS       DAOQVJ010000342         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_76475_length_1933_cov_3.595314, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000342 DAOQVJ010000000
VERSION     DAOQVJ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76475_length_1933_cov_3.595314"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1775)
                     /locus_tag="PKL26_07140"
     CDS             complement(204..1775)
                     /locus_tag="PKL26_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS83505.1"
                     /translation="MTVDPLITAMKTQGRPNDLESANQSLTDENARLKGMIDDCGRIR
                     NELRLSKENLRGFLASLPHSAHLIDREGTVLISSEVSPARLGISLDEYIGTNIFAHLP
                     PGLAAERREHFERILRSRETVSWEDTREGRHYTHYGSPVLDDEGNVDRIAHIALDITY
                     RKRIEDAIRESEEKYRAVVEMSPDAIVIHQDGRIVYANPASAQLFHLPASDVLIGTDA
                     FARIHPDFLAIARENTRIDLEGRDTPPTEIQVTLRDGTLATFEGRGRKLIFQGRPAIQ
                     VVLRDISERKKAEEQLQEFSRNLARANEELELFISIASHDLQEPIRNIVTSTQRMLRD
                     SRESDAGSFEKHLKTIETAALRMHALINSLREYSAVRKRDLQSVSMDNALSSALENLR
                     QLIKETGATVTHDPLPNALADPTRIIQVFQNLIENAIKFRKEGVPLVIHISASRDGDV
                     WEFSVRDNGIGIPSDYYDRIFVLFEQLHQRDVYPGTGLGLALCKKIIERNRGRIWVSS
                     VIGEGSTFFFTLKAE"
BASE COUNT          388 a          533 c          511 g          501 t
ORIGIN      
        1 tcctccatcg ccctgttcca ggcaatcacc tttccctcgc ggtcgatgac cagcgtcgca
       61 tccggcaaaa agtcaataac ctcatcctga aacgcatgcc cggagagttg cattcacctg
      121 ctcctgatca gaggatgtac ggtgattagg taagaagatt cgttcttcct ctcctgcctg
      181 tcaggcgaac agaaaatgaa tgcttattcc gctttcaggg taaaaaagaa cgtactgcct
      241 tctccaataa cagatgatac ccagattcgc cccctattac gttcgatgat ctttttgcag
      301 agggcaagtc cgagtccagt gcccggatat acatccctct ggtggagctg ttcgaagaga
      361 acgaagattc tgtcataata gtcggacgga atgccgatcc cgttatccct gaccgaaaat
      421 tcccatacgt cgccatcacg ggaggcagag atgtgtatga ccagcgggac gccttccttt
      481 cggaacttga tggcgttctc gatgaggttc tggaagacct ggatgatccg ggtcgggtcg
      541 gcgagggcat tcggcagggg atcatgcgtg acggttgcgc ccgtctcttt tatgagctgc
      601 cttaagtttt ccagcgcgct ggaaagcgcg ttatccatgg agactgactg cagatccctc
      661 tttctcacgg ccgagtactc gcggaggctg ttaatcagcg catgcatgcg aagggctgcc
      721 gtttcgatgg tttttaagtg tttttcaaag gaacccgcat cgctttcgcg agaatctcgc
      781 aacatccgtt gtgtcgaggt cacgatgttc cggattggtt cctgaaggtc atgtgaggca
      841 atcgagatga aaagctcaag ttcttcgttg gcccgcgcca ggtttctcga aaattcctgc
      901 aattgttctt ctgccttctt ccgttccgat atatcgcgca gcaccacctg gatcgcgggt
      961 ctgccctgga agatcagctt cctccctctc ccttcaaatg ttgccagggt cccgtcacgg
     1021 agtgttacct gtatctctgt gggtggggtg tctctgcctt caaggtcaat cctggtgttc
     1081 tcccgtgcga tcgcaaggaa atcagggtgg atccgggcga atgcatccgt gccaataagc
     1141 acatctgacg ccgggaggtg gaagagctgc gcacttgcag gattcgcata tacgatccgg
     1201 ccgtcctggt ggattacgat ggcgtccggt gacatctcga cgaccgcacg gtacttctcc
     1261 tcgctttcgc ggatggcgtc ctcgatcctc ttccggtagg tgatatccag ggctatatgg
     1321 gcgatgcggt ccacgtttcc ctcatcgtca agcacggggg atccatagtg cgtgtagtgc
     1381 cggccctctc gcgtatcctc ccaggagact gtttccctgc tccggagaat cctttcgaaa
     1441 tgttctctcc tttctgctgc aagcccggga gggagatggg caaagatatt ggtcccgata
     1501 tactcatcga ggctgatccc gagtcgtgca ggagagactt cactactgat aagcacggtg
     1561 ccttccctgt caataaggtg ggccgagtgg gggagcgagg ccaggaaccc tctcaggttc
     1621 tctttgctca atctcagctc gttcctgatc cgcccacagt catcgatcat ccctttcaat
     1681 ctcgcattct catcagtgag cgattggttt gccgattcca ggtcattcgg gcgtccctgg
     1741 gtcttcatcg ccgtgataag gggatctacg gtcacaatgt catcccgcgg tcgatttgta
     1801 ttgtgtccat tcccagaacg tggcattcac gcttcccctc catctgcccc tgccggatat
     1861 catgataatt caaaaagaga atgggttcgt gtgacctctt attcattttg ggggggaatt
     1921 tcacccactg gac
//
LOCUS       DAOQVJ010000343         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_76637_length_1930_cov_4.513600, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000343 DAOQVJ010000000
VERSION     DAOQVJ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76637_length_1930_cov_4.513600"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKL26_07145"
     CDS             complement(<1..1006)
                     /locus_tag="PKL26_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS83506.1"
                     /translation="MMEFMEGSHAVAHAVKYCRPEVIAAYPITPQTHIVEALADMVAN
                     CQLDAEYMTVESEFSALSACLGASAAGSRVYSATTSQGLALMFEVCFNCAGMRFPVVM
                     SIVNRAMGAPLSIWNDQQDSISLRDSGWLQFYAEDNQEAADLHYLAYKVAEDKNVLLP
                     AFVCFDGFILSHTYEPVDMLTQEQVDNYLPRYSPVQRLDSENPLSFGLFASPDYYMEF
                     RYQIDQAMKGAAKNIASAGSEFGEMFGRDYGDMIESYRLEDADCALVAMGSICGTVKD
                     AIDEMRKDGKKVGLMRLRTFRPFPGKEVRSALSNVSRVAILDKNISLGAKGAVALEVK
                     D"
     gene            complement(1008..1268)
                     /locus_tag="PKL26_07150"
     CDS             complement(1008..1268)
                     /locus_tag="PKL26_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS83507.1"
                     /translation="MALGLGCSAKPGKAKENKTGSWRTFRPEFQHEKCTKCGLCAQIC
                     PEGCIAEDEEGCPVPAYDYCKGCGLCAEECPSQAIEMKKEEK"
     gene            complement(1272..1814)
                     /locus_tag="PKL26_07155"
     CDS             complement(1272..1814)
                     /locus_tag="PKL26_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HNS83508.1"
                     /translation="MRELRIHGRGGQGSVTAAELIAVAAFAGGVYAQAFPAFGVERRG
                     APVQAFVRFDNKKVRLRSQVYEPDYIIVQDSTLIRDVNVFQGLKKDGIVIVNTEKALN
                     YPIPEGVKVITIDATSVALKHLGLPITNTTLMGAFAAASGEIALPALEGAIRERFRGE
                     LADKNVSAARDAYNLVKGVS"
BASE COUNT          380 a          587 c          547 g          416 t
ORIGIN      
        1 catccttcac ctcgagagcc actgcgccct ttgcaccaag agagatgttc ttgtcgagta
       61 tcgccaccct gctgacgttc gagagtgccg atctcacctc cttcccgggg aacgggcgga
      121 aagtgcgcag cctcatgaga ccaaccttct ttccatcctt tctcatctcg tcgatcgcgt
      181 ccttgacagt cccgcagatc gagcccatgg ccacgagtgc acagtccgca tcttcgaggc
      241 gataggactc gatcatgtcg ccgtaatccc tcccgaacat ctctccaaat tccgatcccg
      301 cgctcgcaat atttttggcg gctcccttca tcgcctggtc gatctggtac cggaactcca
      361 tgtagtaatc aggggatgca aagaggccaa aggagagggg gttctcgctg tccaggcgct
      421 ggaccgggga ataccgtggg aggtagttgt caacctgttc ctgggtgagc atgtcgaccg
      481 gctcgtaggt atgggagagg atgaatccgt cgaaacagac aaaggcgggg agaaggacgt
      541 ttttatcctc ggccactttg tatgcaaggt agtggaggtc agccgcctcc tggttgtcct
      601 ctgcatagaa ctggagccat cccgagtccc ggagcgagat ggagtcctgc tggtcgttcc
      661 agatcgagag cggggctccc atcgccctgt tcacgatgga catcacgacc gggaagcgca
      721 tccctgcaca attgaagcag acctcgaaca tcagggcgag gccctgggag gtggtggcgg
      781 agtagaccct tgagccggca gcgcttgcgc cgaggcatgc ggagagggcg gagaactcgc
      841 tctccacggt catatactcg gcatccagct ggcagtttgc caccatgtcc gccagcgcct
      901 ccacgatgtg ggtctgggga gtgatgggat aggctgcaat gacctccggg cggcagtact
      961 tcaccgcgtg ggcgacggca tgcgaccctt ccatgaactc catcatctta tttctcctcc
     1021 tttttcatct cgattgcctg gctcgggcac tcctctgcgc agaggccgca ccccttgcag
     1081 taatcgtatg cgggaacagg gcacccctct tcgtcctcag caatacaccc ttcggggcat
     1141 atctgggcgc agagaccgca cttcgtgcac ttctcgtgct ggaactcggg gcggaatgtc
     1201 ctccacgagc ctgttttgtt ctcctttgct tttcccggct ttgccgagca gccaagaccg
     1261 agtgccatgg ttcaggacac ccctttcaca aggttatacg catcgcgggc tgcggagacg
     1321 ttcttgtctg ccagttcgcc ccggaagcgc tcccggatag ctccctcgag cgccgggagc
     1381 gcgatctcac cgctggcagc ggcaaacgcc cccatcaggg tggtattggt gatggggagg
     1441 cccaggtgct tcagcgcaac agaagtggca tcaatggtaa tgaccttcac accctcgggg
     1501 atcgggtagt tgagagcctt ttcggtgttc acgatcacga tcccgtcttt cttgagtccc
     1561 tggaatacgt tcacgtcccg gatcagggtg ctgtcctgca cgatgatgta atcaggctcg
     1621 tacacctggc ttctcaacct gaccttcttg ttgtcgaacc ggacgaacgc ctggaccggg
     1681 gcacccctgc gctccacccc gaaggccgga aacgcctggg catacacccc gcccgcgaat
     1741 gccgccacgg ctatcagttc tgcggcggtg accgaaccct gtccgccacg gccatgaata
     1801 cgcagctcgc gcaaagaaat cacagtataa ttttacatga ttaatcatac taaaatgttg
     1861 agattttggg ctgcggatcc cgatggaacg ggagtgatcc aggcatattc accccttgaa
     1921 gaaacccggg
//
LOCUS       DAOQVJ010000344         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_76991_length_1925_cov_3.798930, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000344 DAOQVJ010000000
VERSION     DAOQVJ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76991_length_1925_cov_3.798930"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="PKL26_07160"
     CDS             complement(<1..1884)
                     /locus_tag="PKL26_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNS83509.1"
                     /translation="MTVFTDNSGAIVFDDTFLVTHKVETHNSPSALDPFGGALTGIVG
                     VNRDTIGFGLGAKPCINVYGYCVGDPGTEPALFRGRDRERPILSPRRILDGVVHGVGV
                     GGNCSGIPTPQGWCYFHDRYVGKPLVFAGTVGVMPRVHGDEKLYEKRALPGDLIVMVG
                     GRVGKDGIHGATFSSEALDPASPVTAVQIGDPITQKKFSDVIVKEARDQGLYRSITDN
                     GAGGLSCAVAEMAKESNGCHVFLDRVPLKYPGMAPWEIWISESQERMTLAVPEERLDA
                     FMDLMQRREVEATVIGRFTSSGKCVVECHGAVVMDIGLDFLHDGLPKKHLKTTPVVAR
                     NPEPAAPCPDRLDATLLAMLKRKNICAKEFISTQYDHTVQGGHVLGPVQGAGRVQGCA
                     TLTKVVPGSDRGVGLSQGLFPIYSEIDPYRMAIAAIDLAIRGLIAIGVPLDWIALLDN
                     FCWCSSEDPERLWQLKRAAFGCYDGATAFSTPYISGKDSMFNDFSGFDEENNPVKVSV
                     LPTLLISSIGIHPDVTKAVSMDAKFAGDLVYVIGMTAEELGGSEYFMHLGSPGNRVPA
                     LDLDAARERYGRLAKAIDKGLVASAFPVSHGGLLVALAKVSIAGRLGMEIAIPSTIRP
                     DYYL"
BASE COUNT          334 a          628 c          591 g          372 t
ORIGIN      
        1 gaggtagtaa tccgggcgga tggttgaggg gatggcgatc tccattccaa gtctccctgc
       61 gatggagacc tttgccagtg cgacgagaag gcctccgtgg ctgaccggga acgcggacgc
      121 gacgagtccc ttgtctatcg cctttgccag ccgcccatac ctttccctcg ccgcgtccag
      181 gtcaagggcc gggacacggt tccccgggct cccgaggtgc atgaagtatt cggacccccc
      241 gagttcctcc gcagtcatcc cgatgacgta gacgaggtct cctgcgaact tcgcatccat
      301 cgagaccgcc ttcgtgacgt cggggtggat gccaatcgag gagatgagga gcgtagggag
      361 gaccgagacc ttcacggggt tgttctcttc atcgaatccc gagaagtcgt tgaacatgct
      421 gtctttcccg gagatgtagg gagtcgagaa cgcggtggcg ccgtcgtagc acccgaaggc
      481 tgcccgcttc agctgccaga gcctttccgg gtcttcggaa gagcaccagc agaagttgtc
      541 gaggagggcg atccagtcaa gcggcacgcc gatcgcgatg agtccccgta tagccaggtc
      601 gatggccgcg attgccatcc gataggggtc tatctccgag tatatgggga agagcccctg
      661 cgagagcccg acaccccggt ccgatcccgg cacgaccttc gtgagcgtgg cacacccctg
      721 gaccctcccc gccccctgca cgggcccaag cacatgtccc ccctggacgg tgtggtcgta
      781 ctgggtggag atgaactcct ttgcgcagat gttctttctc ttcagcatgg caaggagcgt
      841 agcgtcgagc cggtcggggc agggtgccgc gggctcggga ttcctggcga cgacgggcgt
      901 cgtcttcagg tgcttcttcg gaagcccgtc gtgcaggaag tcaagcccga tatccatcac
      961 gaccgcgccg tggcactcga ccacgcactt tccgcttgag gtgaacctgc cgatcaccgt
     1021 ggcctccact tcgcgtctct gcatcaggtc catgaacgcg tccagacgct cctccggcac
     1081 ggcaagtgtc atccgctcct gggactccga gatccagatc tcccagggag ccatgccggg
     1141 gtacttcagc ggaaccctgt cgaggaagac gtggcagcca ttggattcct ttgccatctc
     1201 cgcgacggca caggagagtc ctccggcgcc gttgtcggtg atgctcctgt acaacccctg
     1261 gtcacgcgcc tccttgacga tgacgtccga gaacttcttc tgggtgatgg ggtccccgat
     1321 ctggaccgcg gtcaccgggc ttgccgggtc aagcgcctct gaagagaacg tggcgccgtg
     1381 gatgccgtct tttcccaccc ggccgccgac catgacaatg aggtctcctg gcagtgcccg
     1441 cttctcgtag agcttctcat cgccgtggac acgcggcatc acgccgactg tccctgcgaa
     1501 tacgaggggc tttcccacgt accggtcgtg gaagtagcac cacccctgcg gcgtcgggat
     1561 gcccgagcag ttgcccccca cgccgacccc atgaacgact ccgtcgagga tcctgcgagg
     1621 tgagaggatt ggcctctcac ggtctcttcc ccggaagagc gcaggctccg tcccggggtc
     1681 gccgacacag tacccgtaca cgttgatgca gggcttagcc ccgaggccga accctatcgt
     1741 gtcccggttc acccccacga tcccggtcag ggccccgccg aaggggtcga gcgccgacgg
     1801 ggagttgtgg gtctcgacct tgtgcgtcac gaggaacgta tcgtcaaaga cgatcgcccc
     1861 ggagttgtcg gtgaacaccg tcacgcatat atcccggtct cctctcttct tccggataat
     1921 attgg
//
LOCUS       DAOQVJ010000345         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_77283_length_1920_cov_4.137802, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000345 DAOQVJ010000000
VERSION     DAOQVJ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77283_length_1920_cov_4.137802"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKL26_07165"
     CDS             <1..712
                     /locus_tag="PKL26_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HNS83510.1"
                     /translation="NHSPIEKLLDTVKSYERLDADILSLAAVASIVGLVGLFLNNVGI
                     IIGAMLIAPMIGPIYAFALHSATGNAMGVLRDILNIIFLILSFILFSLVVTWILGLFI
                     PLEVTGEMLSRTHSSPIYAIMAIFLGFATIVALSKGIPDSIAGVAVAAALLPPALVSG
                     LTVVLYPEGTLGALVLTLQNAIGLMAGSIMATIALQIQPRSYREKAAAKAILRRVAIL
                     LLILVLLLALIPLITVSG"
     gene            complement(709..1824)
                     /locus_tag="PKL26_07170"
     CDS             complement(709..1824)
                     /locus_tag="PKL26_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS83511.1"
                     /translation="MRGVIQKRREYLHLMRACTLERGHFTVTDIQKGAGVPRSTAQDW
                     INRLLDEGCVVIRERKMGRNPAKYAAISALPSSACRRIFTTIDGDSVEIHHECMSSAC
                     AAFCAYHHSHARGILQGVHRDGTLIREWARLGREDIDIGLFPSSAVGVAGVERRGEDI
                     IQHIRCIGGPAYSLTDMMSHAEGVCEVSIQRSGEVVEGSVRTRALAHLIIGIDDTDSR
                     EGGATFALALALLQHLETLKGVLPISHHVVMLNPAVSEKTAGNSCSYIEVAIPPGMYS
                     RVRDRSLVFVEDEALSREWGMAFKQGFQVPQSLRAYGDMARRGIVTREEAAETAAQHH
                     IEVIGGRGIVGALAAVALSGLSHEVLLRAEARIPGTS"
     gene            complement(1796..>1920)
                     /locus_tag="PKL26_07175"
     CDS             complement(1796..>1920)
                     /locus_tag="PKL26_07175"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315144.1"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNS83512.1"
                     /translation="MAGEITLIGKGAGSVETASAIIGDLLYIRDTHAGRHSKTA"
BASE COUNT          354 a          628 c          533 g          405 t
ORIGIN      
        1 gaatcactct cccatagaga aactcctcga caccgtcaaa tcttacgagc ggctggacgc
       61 ggatatactc agcctggcag cagttgcgag catcgtgggg ctggtaggcc tctttttgaa
      121 caacgtgggg atcatcatcg gtgccatgct gattgccccg atgatcgggc ccatctacgc
      181 gtttgctctc cactcagcga caggaaatgc catgggggtc ctccgcgata tccttaatat
      241 tatcttcctc atcctttcct tcatcctctt ctcgcttgtc gtgacgtgga tcctcgggtt
      301 gttcatccct ctcgaggtga caggcgagat gctcagcagg acgcattcaa gcccaatata
      361 tgccatcatg gcgatattcc tcggctttgc aaccatcgtt gcgctctcga agggaatccc
      421 ggacagtatc gcgggagtcg ccgttgcggc ggcactgctg ccgcctgcgc tcgtgagcgg
      481 tcttaccgtg gtattgtacc cggagggaac cctcggtgcc cttgtcctca ccctccagaa
      541 cgccataggc ctgatggccg ggagcatcat ggcgacaatc gcgctccaga tacagcccag
      601 gtcctacagg gaaaaggctg cggcaaaggc catactcagg cgtgtcgcga tacttctgct
      661 gatcctcgtc cttctcctgg cccttatccc gctgattacg gtcagcggct aactcgtgcc
      721 tggaatcctc gcttcggccc tgagcagaac ctcgtgggat aagcccgaga gtgcgaccgc
      781 cgcaagggca cccacgatcc cccggccgcc aatgacctcg atatggtgct gcgcagcagt
      841 ctctgctgcc tcctccctgg taacgatacc gcgccttgcc atatcgccgt aggcacgaag
      901 gctctggggg acctggaacc cctgcttgaa agccattccc cattcccggg agagggcttc
      961 gtcctcgaca aagaccaggg acctgtcacg tacccgtgag tacatgccgg gcgggatcgc
     1021 gacctcgata taactgcacg agttccccgc cgtcttctca ctcaccgcag ggttgagcat
     1081 gaccacatgg tggctgatgg ggagaacgcc cttcagtgtc tcgagatgct ggaggagcgc
     1141 aagggcgagg gcaaacgtgg ctccaccctc ccggctgtcg gtgtcatcga tcccgatgat
     1201 caggtgggcc agggcacggg tccggacgct gccttccacg acctcccctg atcgttgtat
     1261 gctcacctcg cacacgccct ccgcatggga catcatgtcg gtgagagaat atgccggccc
     1321 cccgatgcaa cggatgtgct ggatgatgtc ctcccctcgc ctctctaccc ctgcgacccc
     1381 tactgcagac gaggggaaaa gcccgatatc gatgtcctcg cgcccaaggc gggcccattc
     1441 tcgaatgagg gtcccatccc tgtgcacgcc ctggaggatc cccctggcat gggagtggtg
     1501 gtacgcgcag aacgcagcac aggcactgct catgcactca tggtgaatct cgacactgtc
     1561 cccgtcgata gtggtgaaga tcctcctgca ggcactggag ggaagcgcac tgatagccgc
     1621 atactttgcg gggttccggc ccatcttcct ctcccggatc accacgcacc cctcatcgag
     1681 caggcggttg atccagtcct gggcggtgct ccgtggaacc cctgccccct tctggatatc
     1741 ggtcaccgtg aaatggccgc gctccagggt gcacgcccgc atgaggtgca ggtactcacg
     1801 ccgtttttga atgacgcccc gcatgagtat cccttatgta gaggagatcc ccaatgatgg
     1861 cactcgcggt ctctacggac cccgcgcctt ttccgatcag tgtgatctcc cctgccatgt
//
LOCUS       DAOQVJ010000346         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_77352_length_1919_cov_4.508047, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000346 DAOQVJ010000000
VERSION     DAOQVJ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77352_length_1919_cov_4.508047"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1469
                     /gene="aspS"
                     /locus_tag="PKL26_07180"
     CDS             177..1469
                     /gene="aspS"
                     /locus_tag="PKL26_07180"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNS83513.1"
                     /translation="MRIPIQEVTPGVERAEIVGFVHELRDLGGLSFLLVRDRTGIIQV
                     TVPKKKVPEAVLSAVRLVSRESVVRVAGTVKAIDKAPGGRELVPETFEIVSLAESPLP
                     LDVVEKVPAELDTRLDSRFLDARRPRVAAVFQVRNACMRAIYEHLFSSGFIQITTPKV
                     VAAATEGGTELFPIAYFEKEAFLNQSPQLYKQMMMGAGFEKVFEIGPIFRAEEHNTTK
                     HLNEATSIDVEVSYADHHQVMALLEEIVIAAYRRVDSECRPFLELLDLPDFEVPSSAF
                     PRLPYEEAITIAQKKTDEPITYGDDIGTAAEKAIGEEMGCHYFIVDWPTAIKPYYAMP
                     YENDASVCRAFDLMHPRMELSSGSERVHQHDLLVRQIQSKGLNPDSFAFYLTPFRYGM
                     PPHSGWGLGAERLVMTMLGLQNIREAVLFPRDRHRLVP"
     gene            1476..>1919
                     /locus_tag="PKL26_07185"
     CDS             1476..>1919
                     /locus_tag="PKL26_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNS83514.1"
                     /translation="MDPFARLPILPTTVVGSFPVERGSGLGGLLDPFRHAVKVAVDAQ
                     VRAGIDIISDGQVRGDMIQAFSSRLPGIRGQDVVNKVLPASGPITVNDTRYALSCHPM
                     VKGILTGPTTLSCGLHISTPVYRDRQDLALDLAAALVPEALALERA"
BASE COUNT          361 a          609 c          587 g          362 t
ORIGIN      
        1 cggttgccgc gcaggccggc atggaggatg ccatcaggca ggagattttc gggactgaat
       61 aacagagtga ggtgaccacc caggatgcct ggattttccc ccggtcccgt ggttgtcttc
      121 ctgccagcgc aggaaaacaa gattatcaat cacggaccat aggtattgga tttcatatgc
      181 ggatacctat acaggaggtg actcccggcg tggaaagggc agagatcgtg ggtttcgtgc
      241 acgagttgcg ggaccttgga ggactgtcct tcctgctcgt cagggatcgc acagggatca
      301 tccaggtgac cgtccccaag aagaaagtcc cggaggccgt gctctccgca gtccgtctgg
      361 tctcccgcga gtccgtggtg agggtggcag ggaccgtgaa ggccatcgac aaagcaccgg
      421 gcggcaggga gctggtcccc gagaccttcg agattgtgag cctcgcagag agcccgctcc
      481 cccttgatgt cgtggagaag gtacccgcag aactggatac caggctcgat tcgcgcttcc
      541 ttgatgcgag gcgtcccagg gttgctgcgg tcttccaggt caggaacgcc tgcatgcggg
      601 cgatatacga acacctcttc tcttccgggt tcatccagat cactacgccc aaggtggtgg
      661 ccgcggcaac cgaggggggg accgagctct tccccatcgc gtacttcgag aaggaggcgt
      721 tcctgaacca gagcccccag ctttacaagc agatgatgat gggggctgga tttgagaagg
      781 tcttcgagat cggaccaatc ttccgggctg aggagcataa caccacgaaa cacttaaacg
      841 aggcgacctc gatcgacgtc gaggtctcgt acgccgacca ccaccaggtg atggcgctcc
      901 tcgaagagat cgtcatcgcg gcataccggc gggtcgactc tgaatgccgc ccgtttcttg
      961 aactgctgga cctccccgat ttcgaggtcc cgtcctcggc cttcccccgc ctcccctacg
     1021 aagaggcgat cacgatcgcc cagaagaaga ccgacgagcc gatcacctac ggggacgaca
     1081 tcggtaccgc ggcggagaag gccatagggg aggagatggg ctgccactat ttcatcgtcg
     1141 actggccaac cgcgatcaag ccctactatg cgatgccgta cgagaacgac gcatcggtgt
     1201 gcagggcatt tgaccttatg cacccgcgga tggagctctc gtccggttcg gagcgcgtgc
     1261 accagcacga tctcctcgtc cgccagatcc agtcgaaggg actgaacccc gacagtttcg
     1321 cattctacct caccccgttc cggtacggga tgccccctca ctcgggctgg ggcctcgggg
     1381 ccgagcggct ggtcatgacc atgctcgggc tccagaacat ccgggaagca gtccttttcc
     1441 cccgcgaccg gcaccgcctg gtaccctgat ctcccatgga cccgttcgcc cgcctcccga
     1501 tcctccccac gacagtcgtg gggagctttc ccgtggagcg gggctcgggg ctgggggggc
     1561 tcctcgaccc gttcaggcac gcggtgaagg tggcggtgga cgcacaggtc cgggcaggga
     1621 tcgacatcat ctccgacggt caggtccgtg gggacatgat ccaggccttt tcttcccgcc
     1681 tccccgggat ccgcgggcag gacgtagtga acaaggtgct cccggcatcg gggcctatca
     1741 ccgtcaatga cacccgttac gcactttcct gccatccgat ggtgaagggt atcctcacgg
     1801 gtccgaccac gctctcttgc ggcctgcata tctccacccc tgtctatagg gacagacagg
     1861 acctggcact tgaccttgcg gccgcccttg tccccgaggc ccttgccctt gagcgggcc
//
LOCUS       DAOQVJ010000347         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_77436_length_1918_cov_3.238325, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000347 DAOQVJ010000000
VERSION     DAOQVJ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77436_length_1918_cov_3.238325"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKL26_07190"
     CDS             complement(<1..606)
                     /locus_tag="PKL26_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNS83515.1"
                     /translation="MTIIRSGIGNKRPVREGQMEIDDIIVGRRYREELGDMEDLKRSI
                     SELGLLQPIVIRKGTNELVAGYRRLKALTELGITSLVEGTHVNLIDIESLVLGEHDEN
                     ICRKDFTISEKVAIFEAVKEEQLRIGMERQILAGTRGPGAGDGAAGDHSGVDGTDDAN
                     AAGKLVNRTHGHARSHAAKVVGLHQNTMRKASEIVAAAKKEP"
     gene            complement(865..1230)
                     /locus_tag="PKL26_07195"
     CDS             complement(865..1230)
                     /locus_tag="PKL26_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83516.1"
                     /translation="MKVEATKMVAKGGPGTADILLSYVDRYMAGLGPYVSSVFFILLL
                     KSDADRRKRGLIRSRGLSINEIADAAGISRRKAIDALKVLKGRWMIVQRKGRGRGNKN
                     HYYFLPMERWSNPGSLEKR"
     gene            complement(1227..1865)
                     /locus_tag="PKL26_07200"
     CDS             complement(1227..1865)
                     /locus_tag="PKL26_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83517.1"
                     /translation="MVTAGERAQAVASYARAFFRSMGADEFALMIENARSSGQLWLVE
                     RLYSRKVIDGVVRRILGRVDRGHYVGDQLNDYIQDATVVLMQLARQEKLDLTKSAGEI
                     ASYICLWIEQRVKRMARKDQRWRFSLLETTDDIEGSAGDIAVEEGTANSWNPGWTEAY
                     HPPGPATEDTSAAGLWEKVCVAEKRISMTASGARKSERNVLSIFGPSRSEKV"
BASE COUNT          352 a          620 c          476 g          470 t
ORIGIN      
        1 gggctccttt ttcgcggcgg ccacgatctc cgaggctttt ctcatcgtat tctgatggag
       61 tcccaccacc ttcgccgcat gggacctcgc atgtccgtgt gtgcgattga ccagtttccc
      121 cgctgcattc gcgtcatcgg tcccatcgac cccggagtgg tcgccggcag caccgtcacc
      181 tgcacctggt ccgcgggtac cggccaggat ctgcctttcc attccgatcc tcaattgctc
      241 ttccttgacc gcctcgaaga tggccacttt ttccgatatc gtgaagtcct tcctgcagat
      301 gttctcgtca tgctcgccaa gtacaaggct ctctatgtcg atgaggttga cgtgggtgcc
      361 ctcgacgagg gaggttatgc cgagctccgt gagggctttg agcctccgat agccggcaac
      421 aagctcgttc gttccctttc ggatcacaat cggctggagg agccccagtt ccgatatgga
      481 gcgcttcagg tcctccatgt ctccgagttc ctccctgtaa cgcctgccaa caattatatc
      541 atcaatctcc atctgcccct ccctcaccgg ccgtttgttc ccgattccgc tgcgtatgat
      601 ggtcatgtcg tgcactctat ataatatatc cttctcacgc gacgcaccgt catttcaggg
      661 atctcttcgc gtggcgggat ggaacacccc tgtacaattg gggcaggggc cacaagtggc
      721 ccctatcccg tggatcacgg tggtttcggg gattatcgcg caccgcccca aggtaaaggt
      781 gcacaggatg catgttggag atgaatgcct tcgggtatcc aggccctttc ccgcgggcat
      841 ggacccggta gcggtgctcc aggctcaccg tttttcaaga gatccggggt tggaccatct
      901 ctccatggga agaaaatagt aatgattctt gttccccctt cctcttccct tgcgttgcac
      961 gatcatccac ctgcccttga ggaccttgag ggcatcgatg gccttcctcc ttgatattcc
     1021 cgcggcatcg gcgatctcgt tgatggacag gcccctgctt ctgatgagtc ccctcttccg
     1081 tctgtcggcg tcggacttca ggagaagaat gaagaaaacg gagctcacgt agggcccgag
     1141 ccctgccatg taacggtcca cataggacaa caggatatcc gcggttccgg gaccaccctt
     1201 tgccaccatc ttcgtcgcct cgaccttcac accttctccg agcgcgaggg accgaatatg
     1261 gaaaggacat tgcgttcgct cttgcgcgca cccgaggcgg tcatcgaaat ccgtttttca
     1321 gccacgcaga ccttctccca gagtccggcg gccgacgtgt cttccgtggc cggtccgggc
     1381 gggtgatatg cttcagtcca tcccggattc caactgttag cggtcccctc ttccacggcg
     1441 atgtcaccgg cggaaccctc gatatcatcc gtggtttcca agagcgagaa cctccaacgt
     1501 tgatcctttc tggccattct cttgaccctc tgctctatcc acaaacagat gtaggaagcg
     1561 atctctccag cggatttcgt gaggtcgagc ttttcctgtc tggccaattg catcaggacc
     1621 accgtcgcat cctgaatgta gtcattcagt tgatcgccaa cataatgtcc cctgtcgacc
     1681 ctgccgagga tcctcctgac gacaccatca atgacctttc tcgaatacag cctctccacc
     1741 agccacagct gacccgatga tctggcgttt tctatcatca gggcaaactc atcggcgccc
     1801 atggacctga agaaggccct ggcataggac gcaactgcct gtgcccgctc cccggcagtc
     1861 accatcgggg acactccctg ctccatacgc atcactttca gatctccttt tcaatctt
//
LOCUS       DAOQVJ010000348         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_77773_length_1913_cov_4.440797, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000348 DAOQVJ010000000
VERSION     DAOQVJ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77773_length_1913_cov_4.440797"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PKL26_07205"
     CDS             complement(<1..1011)
                     /locus_tag="PKL26_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNS83518.1"
                     /translation="MDTMMRKERIDRGLKVRTEGIYAITSAREILYLMGPRILLIVGL
                     LALPFVFELAPYWKKVINIMCAYALLALSFDFLANYVGLVCLGGAFFTGIGGYFAAIF
                     NVELGMPVWLSMPLAGVAGAIFCTVALLPCLPLRGVYFAIVTLMYPLLFTRIIEALDI
                     LGGTNGMTGLDALPNAYVENYVIIVVALVFLFAMRRFVNEDIGLVLRGVKDNDQSVRA
                     SGISVTRMRIIGVFIASLLGCFAGAYIAHLYMWAGISLFALDFSIIPIAAVVIGGGGT
                     LVGALIGAFILVPLSEVLRAFGTFRIVIYCAILTGFIVFKSEGIMIYLQRKYHQFERW
                     VKV"
     gene            complement(1014..1883)
                     /locus_tag="PKL26_07210"
     CDS             complement(1014..1883)
                     /locus_tag="PKL26_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNS83519.1"
                     /translation="MELVVYGIINSITLSLISLGFTLVYSISRVPNFAHGALYVAGGY
                     LTWVFLNHFLGIPYFLAILLGVIITSAVGAAIYQFILVRIRGMEISEIIATYAIGLAI
                     LEGLRWGGLRGGTFVLPAFFPGSVNILGASVDYQRLIIVGAGVLTFIILYLFTRFTKV
                     GLAFRGIAQDERAAMMLGINSDMTAVLSLAIGSALAGLAAILLLPLGNIVVEGGYNVL
                     VFAVAVCVIGGLGSWGGVIVASFIIGFAQILTEYFISAHYQMVVALLAIIITLLVKPS
                     GIFGKQKELEERV"
BASE COUNT          503 a          560 c          491 g          359 t
ORIGIN      
        1 tactttcacc cacctttcaa actggtgata tttcctctga aggtagatca tgatcccctc
       61 gctcttgaac acgatgaatc ccgtgaggat ggcacaataa ataacgatcc tgaaggtccc
      121 gaaggccctg agtacctccg atagaggcac caggataaag gcaccgatca aagctcccac
      181 cagggtcccg ccgcccccta tgacgaccgc ggcaatggga atgatcgaaa aatcaagggc
      241 gaagagcgag atgcctgccc acatgtacag gtgggcgatg taggcgcccg cgaaacatcc
      301 cagcagggac gcaatgaaaa ccccgatgat cctcatgcgc gtaacgctta tacccgacgc
      361 cctgacggac tggtcattgt cctttacacc tcgcaggacg agacctatgt cctcgttcac
      421 gaacctccgc atggcgaaga gaaagacaag ggccacaacg atgatcacgt agttctccac
      481 ataggcattg ggaagtgcgt cgaggccggt catgccgttc gtgccgccga gtatgtccaa
      541 cgcctctatg atcctcgtga acaacaacgg gtacatgagg gttacaatgg caaaatagac
      601 acctcgcaac ggaaggcatg gcaacagggc aaccgtgcag aatatcgctc ccgcaacccc
      661 ggccagcggc atcgaaagcc agacaggcat ccccagttcc acgttgaaga tcgccgcgaa
      721 gtatcccccg attccggtga agaacgcgcc tccaagacaa acaaggccga catagttcgc
      781 caggaaatcg aaggatagcg cgagcagcgc atatgcgcac attatgttga tgaccttttt
      841 ccaatatgga gcaagctcga acacaaaagg aagggctaga agtcccacga tgagcaggat
      901 tcttggcccc atcagataga ggatctctcg ggcggaggtt atcgcgtaga taccttccgt
      961 cctcaccttg agtccccggt cgatcctctc ttttcgcatc atcgtatcca tggctacacc
     1021 cgctcttcca gttctttctg tttaccgaat atccccgatg gcttgacgag cagtgtaatg
     1081 ataatggcga gaagggcaac gaccatctgg tagtgggccg agatgaaata ctccgtcagg
     1141 atctgggcaa agccgatgat gaaggaggcc acgatcactc ctccccagct tcccaatccc
     1201 ccgatgacac agacggcgac ggcaaagacg aggacattgt accccccttc cacgactata
     1261 ttccccagag gcagaagaag tattgcggca agacccgcca gagccgagcc tatggcaagg
     1321 gagagaacgg ctgtcatatc ggaattgatg ccgagcatca tcgccgctct ctcatcctgg
     1381 gcaatgcccc ggaaggcaag gcccaccttt gtaaatcgcg tgaacaggta aagaatgatg
     1441 aaggtcagca caccagcccc gacgatgatc aggcgctggt aatccacgct cgcaccgagt
     1501 atattgacgg aaccgggaaa gaaagcgggg agcacgaatg ttcctccccg cagaccgccc
     1561 cacctgagcc cttcaaggat cgcgagcccg atcgcatatg tcgcgattat ctccgatatc
     1621 tccatgcccc tgatccgcac cagtatgaac tggtagatcg cggcccccac ggcgctggta
     1681 atgatcaccc ccagaaggat cgccagaaaa taggggatcc cgagaaaatg gttcagaaag
     1741 acccatgtga gatatccccc ggcgacatac agggccccgt gggcaaaatt cgggaccctg
     1801 cttatgctgt acacaagggt gaagcccagg gatatcaggg agagggtgat gctgttaatg
     1861 atcccgtata cgacaagctc catatgtccc ctctttcttg atttacttca tcc
//
LOCUS       DAOQVJ010000349         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_77931_length_1911_cov_4.161638, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000349 DAOQVJ010000000
VERSION     DAOQVJ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77931_length_1911_cov_4.161638"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..794)
                     /locus_tag="PKL26_07215"
     CDS             complement(237..794)
                     /locus_tag="PKL26_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNS83520.1"
                     /translation="MAFDTIRSNKSAQIVLGLLIGIAFGFLLQKGGVTEYAVIIGQLL
                     LTDFTVVKVMLAAILVGMIGIYAMKAAGLVRLHSKGGSVGATVIGGLIFGAGFAVLGY
                     CPGTAAGAIGSGALDAVVGVLGIVIGAGIFARLFPYLDRVILNRGAFPADTIPELLGV
                     RPALVVGIVAVLIVGVLAMLAMLGL"
     gene            complement(798..1331)
                     /locus_tag="PKL26_07220"
     CDS             complement(798..1331)
                     /locus_tag="PKL26_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNS83521.1"
                     /translation="MFTWLAMATWSPYIVGAGIGILVWVAFLISDRPLGASTAYAKTA
                     GMVESALSRKKAAAMPYYRKFTPTVDWQWMIVVGIVIGAFLSAWISGTFSVFIVPPLF
                     AGAFSADPLLRIVVALIGGVLMGIGARWAGGCTSGHGISGTLQLSVASWVAVVCFFAG
                     GIFVAGLMFGFPFFSGV"
     gene            complement(1385..>1911)
                     /locus_tag="PKL26_07225"
     CDS             complement(1385..>1911)
                     /locus_tag="PKL26_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS83522.1"
                     /translation="VHIPGAWHIDLSGNFATQAGWILPPGQDILLVVESRNQAEEAAL
                     QLRRVGFDRVPGFLEGGMLSWGSLNLRINRVPVISAEEAHSMVSSGEAVLVDARSKEE
                     WEAGHADRSMHIPWHDLRTRHAELDPARHTIVMCKGGQRASIAASILKMHGFATVSNL
                     AGGYTSYQRAGFAP"
BASE COUNT          455 a          614 c          508 g          334 t
ORIGIN      
        1 tgtctgcttt cccctgcccg caaagactgc gccatgcaga agagatccgc cccgggtcgc
       61 attaaacctt ttctgttggg ggtgcctccg cgcgatttct gcggtttgaa cgattgggat
      121 ggctcgaata aactcaccaa tccataaagg aaggtgtaac acccgtgtgc agagatcagg
      181 agaaaacccg ggttcccaaa tcccctgtct cgttcgctgt aaaaaaagag cgggaatcag
      241 agacccagca ttgcaagcat ggcgagcacc ccgacaatga ggaccgctac gattccgacc
      301 accagcgccg ggcggacccc gagcagctcg ggtatggtat ctgcaggaaa cgcaccccgg
      361 ttcagtatca cccggtcaag gtacgggaag agccgggcga agattccggc gccgatcacg
      421 atccccagga cccccaccac cgcatccagg gcacccgatc cgatcgcacc tgcagcagtg
      481 ccgggacagt agccgagaac cgcaaaccct gcaccgaaga tcaatccccc gatcaccgtc
      541 gcaccaaccg aaccaccctt ggaatgaagc cggacaaggc ccgcagcctt catggcatag
      601 atcccaatca tcccaacgag aatcgcggca agcatcacct tcacgacggt gaaatcggtg
      661 aggaggagct ggccgattat caccgcatac tctgtcaccc cgcctttctg gagaagaaag
      721 ccaaacgcga tgccgatgag gagcccgaga acgatctggg cacttttatt cgacctgatc
      781 gtgtcaaatg ccatggttca gacccccgaa aagaacggga acccaaacat caggccggct
      841 acgaagatcc cgcctgcaaa gaagcagacc accgcgaccc agctcgcgac cgagagctga
      901 agcgtccctg atatcccgtg acctgacgtg cagccaccgg cccaccttgc cccgatcccc
      961 atcaggaccc ctccgatgag cgccacgaca atgcgcagga gcggatccgc agaaaatgct
     1021 cccgcaaaga ggggtggaac aataaaaacg gaaaaagtcc ctgatatcca ggcagagagg
     1081 aacgctccta tcactatacc gacaacaatc atccactgcc agtcaacggt cggtgtgaat
     1141 ttcctgtagt acggcattgc cgctgccttt tttcttgaga gtgccgattc caccatcccg
     1201 gcagtctttg cgtatgcggt cgatgcaccg agcgggcggt ccgagatgag gaacgcaacc
     1261 cacacgagaa ttccaatccc cgcaccgacg atgtacggcg accaggtcgc catcgcaagc
     1321 catgtgaaca tgaatgatct ccggtatcag ggaggattgg gttcttctgt cataatggct
     1381 cccatcaggg cgcaaaaccc gcccgctgat acgaggtgta tccgcccgcg aggttagaga
     1441 ccgtcgcaaa gccatgcatt ttcaggatgc ttgccgcgat gctcgccctc tgtcctccct
     1501 tgcacatcac gatggtatgc ctggcagggt caagctcggc atgccgtgtc ctgaggtcgt
     1561 gccaggggat atgcatggac cgatcagcat ggccggcctc ccactcctcc tttgacctgg
     1621 catcaacgag caccgcctct cctgaactga ccatggaatg agcctcctct gccgatatga
     1681 ccggcacgcg gtttatccgg aggttcaatg acccccatga aagcattccg ccctccagga
     1741 accccggcac ccggtcaaat ccgacgcggc ggagctgaag cgccgcttct tccgcctggt
     1801 tcctggactc gacgaccagg agaatgtctt gccctggcgg gagaatccag cccgcctgcg
     1861 tggcaaagtt tccggaaagg tcgatatgcc aggcaccggg aatgtgcact c
//
LOCUS       DAOQVJ010000350         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_78071_length_1909_cov_3.645092, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000350 DAOQVJ010000000
VERSION     DAOQVJ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78071_length_1909_cov_3.645092"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKL26_07230"
     CDS             complement(<1..229)
                     /locus_tag="PKL26_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase family protein"
                     /protein_id="HNS83523.1"
                     /translation="MILVPVTYKGGIYRHDEIIDLIEDLGGYIIQKHIIAQEVVLQAL
                     VPKEDIDLIRKVGKPLTGEITPSPLVGTEIAV"
     gene            complement(226..807)
                     /locus_tag="PKL26_07235"
     CDS             complement(226..807)
                     /locus_tag="PKL26_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HNS83524.1"
                     /translation="MEAVEYFEVECPEKAGGEYYSRIADVVLLDHNLIRVIQKLHIFI
                     DPRVPLFIATGITRKLPGTVKVRDFSDVNRKERKVTISIGDEAYLAPLLRILWEKFGK
                     ERVEQPDRFTIMLDIGDDESEGLEEIPVFDPSESIYKDLIYALQVIQPEGFKVRRQYY
                     GNGRFYLVASEDTLPEDIEDMVREKFSLMGERL"
     gene            complement(808..>1909)
                     /locus_tag="PKL26_07240"
     CDS             complement(808..>1909)
                     /locus_tag="PKL26_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HNS83525.1"
                     /translation="SFNEFGLEVRSPDLKLAIARAKALVEGRVEADAVFIATCFRCAE
                     AAIVRNELRRYIHENSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREVQQG
                     LTMGVDSGSSTTKAVVMRDNEIIGTGWLPTTGVLQSADDAVSQALSAAGVKRDDLQAI
                     GTTGYGRFLVGEHLKADLIQEELTVNSKGAVFLADTQRGPSTVIDIGGMDNKAISVQD
                     GIPGTFTMGGICAGASGRFLEMTSKRLGVDITELGPLAMKGLSRNVPMNSYCIVFGTQ
                     SLVNALAAGSSPEDVAAAACHSVAEQVFEQQLQEVDIRDPVIMVGGTSLIQGLVRAMG
                     DLLQTEIVVPHHSQYIGAVGSALLASGFIKGD"
BASE COUNT          343 a          600 c          558 g          408 t
ORIGIN      
        1 ccaccgcgat ctcggttccc accaggggcg aaggggtgat ctccccggtc agcggctttc
       61 ccactttccg gatcagatct atgtcctcct ttggcacgag tgcctggagc acgacctcct
      121 gcgcaatgat gtgtttctgg ataatgtagc cgccgaggtc ctcgatgagg tcgattatct
      181 cgtcatggcg gtagataccc cccttgtagg tgacaggaac caggatcaca ggcgctcccc
      241 catcaacgaa aatttctccc tgaccatgtc ctcgatatcc tcggggagcg tgtcctcgct
      301 tgcgacgagg tagaaccgcc cgttcccgta atactgcctc cgcaccttga acccctctgg
      361 ctggatcacc tgcagggcgt aaatcaggtc cttgtagatg gactcgctcg ggtcaaagac
      421 cgggatctct tcgagacctt ccgactcatc gtcgccaata tcaagcatga tggtgaagcg
      481 gtcaggctgt tcgacccgct cctttccgaa cttctcccag agtatccgca gcagcggtgc
      541 aaggtatgcc tcgtcgccga tggagatggt cactttccgc tctttcctgt tcacatcgga
      601 aaaatcgcgc actttcacag ttcccgggag cttcctcgtt atcccggtcg cgatgaaaag
      661 ggggacccgg gggtcgatga agatgtgcag tttctggatc acccggatga ggttatggtc
      721 gaggagcacc acgtcggcga tgcggctata gtattctccg ccggcctttt cagggcattc
      781 cacctcgaaa tactccactg cctccatcta gtctcccttg ataaaccctg atgccaggag
      841 tgcggacccc accgcgccga tgtactggga gtggtgcggg accacgatct cggtctggag
      901 gaggtctccc atggcccgga caaggccctg gataagggag gtccccccaa ccatgatcac
      961 cgggtccctg atgtccacct cctggagctg ttgctcgaag acctgctctg caacgctgtg
     1021 gcaggcggcg gcggccacgt cttcgggact gctgcccgcg gccagggcgt tcaccaggct
     1081 ctgtgtgccg aatacgatgc agtagctgtt catggggaca ttgcgcgaga gacccttcat
     1141 tgccagcgga ccaagctccg tgatatccac gccgagcctc tttgatgtca tctcgaggaa
     1201 ccggccgctc gctcctgcgc agataccccc catcgtgaac gtcccgggga tgccgtcctg
     1261 gacactgatg gccttgttgt ccattccccc gatgtcgatc acagtcgatg gacctctctg
     1321 ggtatcagcc aggaagaccg cacccttgga attgaccgtc agttcttcct ggatgaggtc
     1381 cgccttcagg tgctccccga ccaggaaccg gccgtatccg gtggtcccga tcgcctgcag
     1441 atcgtcgcgt ttcacgccag cggcagagag ggcctgggat accgcatcgt cggcgctctg
     1501 gaggactcca gtggtgggaa gccacccggt gccgatgatc tcgttgtcgc gcatcaccac
     1561 tgccttcgtg gtgctggaac cggagtcgac gcccatggtg agcccctgct gaacttcacg
     1621 tgcaaggagc gcccttctcc gggcgatggt ggtcagggcc tccatgcggg tgagaagggt
     1681 ccctgcggtg gtgcgctcgg tgaaggagta actgaccacc ggtagccggg agttctcatg
     1741 gatgtagcgg cgaagctcgt tcctgacgat tgccgcctcg gcgcacctga agcaggttgc
     1801 gatgaagact gcgtccgcct ctacccttcc ctccacgagc gccttggcgc gggcgatcgc
     1861 gagcttgagg tcagggcttc gcacctcgag accgaactca ttgaacgaa
//
LOCUS       DAOQVJ010000351         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_78196_length_1907_cov_5.561015, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000351 DAOQVJ010000000
VERSION     DAOQVJ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78196_length_1907_cov_5.561015"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="PKL26_07245"
     CDS             complement(<1..1525)
                     /locus_tag="PKL26_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83526.1"
                     /translation="MVAGEITRALSLLFESGQVIEVRAITEEGMASGYFDDPEALAKS
                     IAPLDASGTAGIYVTLNEVNPALLSRRANRIKMRLSKKDATTADTDIIHRRWFPIDID
                     PVRPSGVSSTDEEHQAAIVRAAEIAAYLGRERGWPDPVMADSGNGAHLLYRIDLPNDD
                     ESRDLVKRCLEVLETMFSNSAVTVDTANFNAARIWKMYGTVSRKGDHTEDRPHRKSLV
                     LSGVDQVEVIEKEALRQLAEALPCAPPETKGYKNILDLREWLVKHGVGIAHEKPYQGG
                     TLYILDECPFSGSHRDGAFAIQFPSGAIYAGCHHASCGGGEQRWKELREKFEPQKEKS
                     RRPPARPPSGSRTGASPPINLGELPGREEALAVLHHGNPKQSMLRTFALDHEGDEVVA
                     ECMIMSLASRSVLNTNGLHVSVTGESGKGKSHTFATMLRQVPERLRLEGAMSNKALFY
                     LEDLQPGSVIVLDDRALSEDMAEILKGVTTSFRKPFIYRTVSKDRRPQFCTIPERCIW
                     "
     gene            complement(1634..>1907)
                     /locus_tag="PKL26_07250"
     CDS             complement(1634..>1907)
                     /locus_tag="PKL26_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83527.1"
                     /translation="TVVRSSEPDALVHLVAWKLTRGTLIIRVVTTRRALTGASEVAHL
                     WREDIDRLKSESVPANSEVHLWVYTDRKGWHRYQVLTGGISEVEIL"
BASE COUNT          333 a          597 c          584 g          393 t
ORIGIN      
        1 accagatgca ccgctccggg atggtgcaga actgcggcct gcggtccttg ctcacggtcc
       61 ggtaaatgaa cggcttgcgg aaggaggtgg tgaccccctt caggatctcg gccatgtcct
      121 cggagagggc acggtcgtcg agcacaatca cgctgcccgg ctgcaggtct tccaggtaga
      181 agagggcctt gttgctcatc gccccctcaa gccgcagccg ctccgggacc tgccggagca
      241 tggtcgcaaa ggtgtggctc ttgcccttcc cgctctcgcc ggtcacggag acgtggaggc
      301 cattggtgtt caggaccgac cgggaggcga gcgacatgat catacactct gcaaccacct
      361 cgtcgccttc atggtcgagg gcgaaggttc ggagcatcga ctgtttcggg ttgccgtggt
      421 gcaggactgc aagggcttcc tcgcgtcccg ggagctcgcc gaggttgatc ggtggagagg
      481 ccccggtccg ggatccggaa ggtgggcgtg ccggaggcct cctgctcttc tccttttgag
      541 gttcgaactt ctcgcggagt tccttccacc gctgctcacc gccaccgcag gaagcatggt
      601 ggcaccctgc atagatggcc ccgctcggaa actgtattgc gaacgccccg tcccggtggg
      661 agccggagaa cgggcattcg tccagaatgt acagcgttcc accctggtag ggcttctcgt
      721 gggcaatgcc gacgccatgc tttacaagcc attcccggag atcaagaatg tttttatacc
      781 cctttgtttc cggtggagcg cacggcaatg cttcggccaa ttgacgtaat gcttcttttt
      841 caatgacctc cacctgatca acacctgata gcacgagcga ttttcggtgc gggcggtcct
      901 cagtatgatc accttttcgc gagacggttc cgtacatctt ccagatacgg gccgcgttga
      961 agttcgcagt gtccacagtg accgcgctgt tggaaaacat ggtttccagc acctccaggc
     1021 atcgcttgac gaggtcccgg ctctcgtcgt cgttcggaag gtcgatccgg tagagcaggt
     1081 gcgccccgtt gcccgagtcg gccataacag ggtccggcca cccccgctcc ctcccaagat
     1141 acgcggcgat ctcggctgcc cggactatcg ctgcctggtg ctcctcgtcg gtcgacgaga
     1201 ccccggatgg cctcaccggg tcaatgtcga tggggaacca gcgccggtgg ataatatcag
     1261 tgtccgcggt cgttgcgtcc ttcttcgaga gtcgcatctt gatgcgattc gcccgccgcg
     1321 aaaggagcgc cgggttcact tcgttcaggg tgacgtaaat gccggcggtc ccgctcgcgt
     1381 ccagcggagc tatcgatttg gccagggcct cggggtcatc gaagtacccc gatgccatcc
     1441 cctcctcggt aatcgcacgg acctcaatca cctgcccgga ttcgaagagg agggagagtg
     1501 cccgggttat ctctccggcc accatctggc atcaccgggg gagatcatgc ggcagctcgc
     1561 ggtgtctctt caataagatg gtcatcgacg gcgccactgt cgtgcaggca gttgaggcgc
     1621 accaatgagg cgatcataat atctcgacct cggagatacc gccggtaagc acctgatatc
     1681 ggtgccaacc cttccggtca gtgtagaccc acaagtggac ctcgctgttg gccgggaccg
     1741 actcggactt gaggcggtcg atgtcctccc gccagagatg cgctacttcg gaggccccgg
     1801 ttaacgccct ccgggtggtg accacccgga tgatgagggt tcccctcgtc aacttccatg
     1861 cgaccaggtg gaccagggcg tcgggttcac tcgaccggac caccgtg
//
LOCUS       DAOQVJ010000352         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_78346_length_1905_cov_3.104324, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000352 DAOQVJ010000000
VERSION     DAOQVJ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78346_length_1905_cov_3.104324"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKL26_07255"
     CDS             complement(<1..1197)
                     /locus_tag="PKL26_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HNS83528.1"
                     /translation="MRLSSALMLSLALGLIILHSAGAPNVIGPEDQIFIGEEGLDISA
                     ALPADVNQIAWFSPGSNIRADAPALLLTIGSRQNFYVSPSQFGHATGTWYAWNQTIGD
                     PAFQVLYPSLNMRVWNQETGMDISGTSIAPGTTVNFRIETNMFPITQRAGYDPATDGY
                     ISVLLISPTGATLTAVADTNGTMIPLQRLDVNTLPWYWVPPGSDAGWDSSAMSETGAR
                     LYPTGTYTARLDYNVNRLQDNLQGVPGIGRPIPVHVTLASDRVAITTSGTEIVRGNPF
                     TVTITGVPGADYYLWVRNTGSMTGNPGNQPPLITPNQEGVRMDPSNGPYPIGSYPIST
                     RQGATIRDDVPLSPEGGTRYYALVTLPSSGTRTVQWQTSSATDDGRYLFQAERTVAGR
                     MSSAETS"
     gene            complement(1646..>1905)
                     /locus_tag="PKL26_07260"
     CDS             complement(1646..>1905)
                     /locus_tag="PKL26_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS83529.1"
                     /translation="DYQFPLNILHDEGIYANVRIYPAIWEATYESAEEAARHWVTLHY
                     PGLEDLSPVIDFFSRVLRRDTSGRLVHSAIRRTAVIWWTKE"
BASE COUNT          411 a          492 c          571 g          431 t
ORIGIN      
        1 gctggtttcc gcggatgaca tccgcccggc aacggtgcgc tctgcctgga ataaataccg
       61 cccgtcatcc gtggcagagg acgtctgcca ctggacggta cgggtcccgc ttgatgggag
      121 ggtcacgagc gcataatagc gtgtcccgcc ttcaggcgag agaggcacgt catcccgaat
      181 tgttgctccc tgtctcgttg atatgggata cgatccgatg gggtaagggc cgttggaagg
      241 gtccatccgt accccttcct ggttcggagt gataagaggc ggctggttgc cgggattgcc
      301 ggtcatactc ccggtattcc tgacccagag atagtagtct gcaccaggca cccctgtgat
      361 agtgacagtg aacgggtttc cacgcacaat ttcggtacca ctcgtcgtga tagcaacccg
      421 gtcagatgcg agggtgacat ggacagggat tgggcggccg attccgggaa caccctggag
      481 attgtcctgc aacctgttca cattgtaatc caggcgtgca gtatacgtcc cggtggggta
      541 gaggcgggcc cctgtctcgc tcatggcaga ggagtcccac ccggcatcgg agcctggtgg
      601 gacccagtac caggggagag tgttgacatc aaggcgctgg agaggaatca tagtgccgtt
      661 tgtatcagca acagcggtca gtgtcgcccc cgtgggggat atgaggagca ccgagatata
      721 cccgtctgtt gcgggatcgt agccggcccg ttgtgtgatc gggaacatgt tggtctcgat
      781 cctgaagttc acagtggttc ctggcgcaat gcttgtgccg gatatatcca tcccggtctc
      841 ctggttccat actctcatat tcagcgaagg atacagcacc tggaatgctg ggtcgccgat
      901 agtctggttc catgcgtacc aggttcccgt tgcgtgcccg aactggcttg gtgaaacata
      961 gaagttttgc ctgctcccga tcgtgagcaa caatgccggt gcatcagccc ggatgttcga
     1021 ccctggggag aaccaggcaa tctggttcac gtctgctggt aaggcagcag agatatccag
     1081 tccttcttcc ccgataaata tctggtcttc gggcccgatg acattgggtg ctcctgcgga
     1141 gtgaagaata ataaggccga gtgccaggct gagcatcaaa gcagatgata gtctcattgg
     1201 tattcgattg agatccgtct caatacctcg tgtgtgcaga ctcatggttc ccgtcactcc
     1261 atagaattcc tgcggaaacg atccaacagg tgaccttgcc tctgatggag agtagggtgt
     1321 gagtgggtat aaaaaaccac tgatggtccc atgtgatgcg ggtggtgcca tggcaggatg
     1381 aacaaaaacc ctttcctttt cataccatgg gtgcggaatt attctgaacg aaaaattacg
     1441 ctctcgaaaa gaaccgtccg gactgcatgg gtgtgcgaac tgacatcaga acgggcagga
     1501 tgagatgaat gagagttgcc cttttatcga tgagatgggg taccatggcc acctgtagcc
     1561 gaacggtatg catcatatca tttctgtcaa gggaggttcc ccatcaaatg ggtgaccgtg
     1621 acctcgctga aagttcatcc acgattcact cttttgtcca ccagatcacc gcagtacgac
     1681 ggatcgcgga gtgcaccagc ctgccagagg tgtcccggcg caacacacgt gagaagaaat
     1741 cgatgaccgg cgagaggtcc tcgagtcccg ggtagtgcag cgttacccag tggcgggccg
     1801 cttcttcggc tgactcgtag gtggcttccc agatggcggg atatatccgg acattcgcat
     1861 agatcccctc gtcatggagg atattcaggg ggaactggta gtcgg
//
LOCUS       DAOQVJ010000353         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_78480_length_1903_cov_4.399351, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000353 DAOQVJ010000000
VERSION     DAOQVJ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78480_length_1903_cov_4.399351"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1252)
                     /gene="fbp"
                     /locus_tag="PKL26_07265"
     CDS             complement(155..1252)
                     /gene="fbp"
                     /locus_tag="PKL26_07265"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HNS83530.1"
                     /translation="MPKTTVSLIKADIGSFPGHSRTHPLILEKAARMLKEQEGKLLID
                     SFVTHCGDDLELIMTHTRGEDDAEIHALSWKVFTECAKVAKEMKLYGAGQDILSDAFS
                     GNVKGMGPGVAEMEFEERGSDPILLFMADKTEPGAWNFYLYKMFADPFNTAGLVIDPQ
                     MHDGFIFEVHDVIEKRKIEFRTPEESYSLLAYIGAPSRYVIKYVKRKDGLIAASTSTQ
                     RLSLIAGKYVGKDDPVMIVRGQSGLPAVGEIIDPFSIPIIVAGWMRGSHHGPFMPVGL
                     PDANCTRFDGPPRVICLGFQVCNGKLIGPADMFDDPAYDRVRSRCNELADILRAHGPF
                     EPHRLSLSEMEYTTLPAVEKALKGRWVPIKE"
     gene            complement(1344..1787)
                     /locus_tag="PKL26_07270"
     CDS             complement(1344..1787)
                     /locus_tag="PKL26_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83531.1"
                     /translation="MTEKPKDTERTLEEMLRHLMQAAQGGGDSRPLFIGMKIIVPPAG
                     DSPTPPQKARGDSTESPIEVHRVGDRVMLVTEMPGMTPENIQVMFRGDRVFIWARDQE
                     RHYQGSARVPPSQKGSEEISFRHGVLEVSYLPVEEKSEGDSSPHS"
BASE COUNT          340 a          570 c          578 g          415 t
ORIGIN      
        1 tccttgatct ccaccgtgac cttggaatcc accagagaaa agaccttctt tatgggtaat
       61 acgataccgt tcaccatctg cctcttattg gggggtgccg gtatttattg cttttccgcc
      121 ggaacctggc aggtccatat aaaaaaatgc ggggttattc cttgattggg acccagcggc
      181 ccttgagcgc cttctcgact gccgggagcg tggtgtactc catctccgag agagagagcc
      241 ggtgcggctc gaagggcccg tgggcccgga ggatatcggc aagctcgttg caccgggacc
      301 tgacccggtc gtaggcaggg tcgtcaaaca tgtctgccgg gccgatcagc tttccgttgc
      361 agacctggaa cccaaggcag atcacgcggg gcgggccgtc gaagcgggtg cagttcgcat
      421 cggggagccc gaccggcatg aaggggccgt ggtgcgagcc ccgcatccac ccggcgacga
      481 tgatgggaat gctgaacggg tcgatgatct ctccgaccgc aggcagtccg ctctggcccc
      541 gcacgatcat gacagggtca tccttcccga cgtacttccc ggcaatgagg gagagccgct
      601 gggtgctggt ggacgcggcg ataaggccgt cctttcgctt cacgtacttg atcacgtacc
      661 gggaaggggc gccaatatag gccagcaggc tgtagctctc ctcaggtgtg cggaactcga
      721 tcttccgctt ctcgatcacg tcgtggacct cgaagatgaa cccgtcgtgc atctgcgggt
      781 cgatgacgag ccccgcagtg ttgaacgggt cggcaaacat cttgtagagg tagaagttcc
      841 aggcgccggg ctcggtcttg tcagccatga agaggaggat tgggtcggac ccccgctcct
      901 cgaactccat ctccgcgacg cccgggccca ttcccttcac gtttcctgag aatgcgtccg
      961 agaggatgtc ctggccggcg ccgtaaagct tcatctcctt tgcgaccttc gcgcactccg
     1021 tgaacacctt ccaggagagg gcatggatct cggcgtcgtc ctctccccgt gtatgggtca
     1081 tgatcagctc gaggtcgtcg ccgcaatgcg tcacgaagga gtcgatcagg agcttgccct
     1141 cctgctcctt gagcatcctt gcagccttct cgaggatgag cgggtgggtc cgggagtggc
     1201 ccggaaagct cccgatatca gcttttatca gtgaaaccgt ggtctttggc atgaaaatct
     1261 cctggtgtca gttcttacat actaatagat acaggagata aaaaactgtg ggttttttaa
     1321 aaaaacgttg gcggtgctct cgatcaggag tgaggagaag agtctccttc gctcttctct
     1381 tccaccggga gataggagac ctcaagcacc ccgtggcgga aggagatctc ctcggatccc
     1441 ttctgtgacg ggggaaccct ggcactgccc tggtagtgac gctcctggtc gcgtgcccag
     1501 atgaagaccc ggtcgccccg gaacatcacc tggatgttct cgggcgtcat ccccggcatc
     1561 tcggtgacaa gcatcaccct gtctcctacc cggtgcacct cgatcgggga ctccgtcgag
     1621 tccccgcggg ctttctgggg cggggtcgga gagtcgccgg ccggcggaac gatgatcttc
     1681 atccctataa atagcgggcg ggagtccccg ccgccctgcg cagcctgcat caggtgtcgc
     1741 agcatctctt cgagggttct ctctgtgtct tttggtttct ctgtcatctc gctgctcgtt
     1801 ctggctgaat ggacgagagg gaggggggcc cggtcaggcc cggagttcct cctccagccc
     1861 ctgcgtcagc cgcttgatct cgttccagtc cttgaccctc cgg
//
LOCUS       DAOQVJ010000354         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_78564_length_1902_cov_3.455333, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000354 DAOQVJ010000000
VERSION     DAOQVJ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78564_length_1902_cov_3.455333"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKL26_07275"
     CDS             <1..540
                     /locus_tag="PKL26_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS83532.1"
                     /translation="VFFLLAFLFGRHFCGYLCPIGAVQEIAYAAPTPKIRLPWKRALS
                     AIRVIVFILILAAGLGFSISILAFFGIQEFFRLAFSAGFVVFLIIIAISLFVYRPFCR
                     LICPVGAIFQLFASQARWKIRRTDACIECKKCEKVCPTNEAGRDDAKGECYLCGRCID
                     KCPVEGALRYGGKDTPGNK"
     gene            556..906
                     /locus_tag="PKL26_07280"
     CDS             556..906
                     /locus_tag="PKL26_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS83533.1"
                     /translation="MTRESKQDLLGCVFEMKDLVEYQTGAVSSRMIINNKAGSITIFS
                     FDKDEGLSEHTAPYDAVVTILDGECEVWVAGVTHAMKAGQTIIFPAHAPHALSATTRF
                     KMALIMIRGKDVAP"
     gene            903..1199
                     /locus_tag="PKL26_07285"
     CDS             903..1199
                     /locus_tag="PKL26_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HNS83534.1"
                     /translation="MSSDKEGNYCSVCGGIPPDEIRIRKILVDGKETGIDKLDFILDD
                     VARLGLSGDAEIIAELLKRVQVFNYIPTKKADLYAQALLKEYRAHLQAGSGATH"
     gene            1408..>1902
                     /locus_tag="PKL26_07290"
     CDS             1408..>1902
                     /locus_tag="PKL26_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS83535.1"
                     /translation="MEEDVPGAIKQRDGTTYAIVPRTPSGVVTPDELERIANVARKFK
                     IPVIKMTSGQRMVLVGMKKEEIPSVWKDLGMTVGQATAPCVHYVQACPGTETCKYGVQ
                     DSLGLGLEIERMYQEMNLPAKVKIGVSGCPRCCGESYVRDIGIYGSRKGWTVVFGGNS
                     AGRPR"
BASE COUNT          410 a          527 c          573 g          392 t
ORIGIN      
        1 gtcttcttcc tcctggcttt cctcttcggg aggcacttct gcgggtatct ctgcccgata
       61 ggggcggtcc aggagattgc gtatgcggcc cccaccccga agatcaggct cccctggaag
      121 cgtgcgctct ctgcaataag ggtgattgta ttcatcctca tccttgctgc gggtctcggg
      181 ttctcgatct ctatcctcgc gttcttcggc atccaggagt tcttcaggct tgcgttctcg
      241 gcaggatttg tcgtcttcct gattataatc gcaatctccc tctttgtcta ccggccattt
      301 tgtcgcctta tctgcccggt cggagctatc ttccagcttt tcgccagcca ggcacggtgg
      361 aaaatacggc ggacggatgc ctgtatcgag tgtaaaaagt gtgagaaagt ttgccctaca
      421 aacgaggcgg ggcgtgatga tgcaaagggc gaatgttacc tctgcgggag atgcatcgat
      481 aaatgtccgg tcgagggggc actccggtac ggcgggaaag acaccccagg aaacaagtga
      541 caggaagcga tcgccatgac acgtgaaagt aaacaggatt tgcttggatg cgtcttcgag
      601 atgaaggacc tcgttgagta ccagacagga gccgtttcga gcaggatgat catcaacaac
      661 aaggcaggga gcatcaccat cttttcgttc gacaaggacg aggggctctc ggagcatacc
      721 gcaccctacg acgcggtggt gaccatcctc gacggtgagt gcgaggtgtg ggtcgcaggg
      781 gtgacccacg cgatgaaagc ggggcagacc atcatttttc ccgctcacgc cccccacgcg
      841 ctctcggcta ccacccggtt caagatggcg ctcatcatga tccgcgggaa agatgtggct
      901 ccatgagcag cgacaaggag gggaattact gctcggtgtg cggcggaatc ccccctgacg
      961 agatcaggat ccggaaaata ctcgtggacg ggaaggagac tggcatcgac aagctggact
     1021 tcatcctcga tgacgtggcc aggctcggcc tctcgggaga tgccgagatc atcgccgagc
     1081 tcctgaagcg tgtgcaggtg ttcaactaca tcccgacgaa aaaggccgac ctctacgccc
     1141 aggcgctcct gaaggagtac cgggcccacc ttcaggctgg atccggcgcc acccattgac
     1201 aattctccgg ggatccgaac cgggcaatcc ccgtgatttc tttttctgca tgtccaggcg
     1261 gatgttccag gctacaagta gcgggaatat ccgtccgggg acagtgaggc cgggcgtgcc
     1321 gacctccctc tgcccaatcc tcaccgggaa tgttgttact ctgccagtga ctatatgttt
     1381 attccttccc ggctgcaatc ctcatgcatg gaagaagacg taccaggtgc aatcaaacag
     1441 cgagatggaa ctacctatgc aatagtcccc cgcacacctt ccggagtggt gacgccagat
     1501 gagctcgagc ggatcgcgaa tgttgccagg aaattcaaga tcccagtgat caagatgacc
     1561 tcgggccaga gaatggtgct cgtcgggatg aaaaaagagg agatcccttc tgtctggaag
     1621 gaccttggga tgacggtcgg gcaggcgact gccccctgtg tccactatgt ccaggcatgc
     1681 ccgggcaccg agacctgtaa atacggggtg caggactcgc tcggactcgg cctcgagata
     1741 gagaggatgt accaggaaat gaacctcccg gcaaaagtga agataggcgt ctccgggtgc
     1801 ccccgctgct gcggcgagag ttatgtccgg gacatcggga tatacgggag caggaagggc
     1861 tggacggtcg ttttcggggg gaacagcgcc ggcaggccga gg
//
LOCUS       DAOQVJ010000355         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_78868_length_1897_cov_4.285016, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000355 DAOQVJ010000000
VERSION     DAOQVJ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78868_length_1897_cov_4.285016"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PKL26_07295"
     CDS             complement(<1..1278)
                     /locus_tag="PKL26_07295"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HNS83536.1"
                     /translation="MPSCFTCPVLLSGTAVLILVVFLVAAGCIATPQAPSLSVTPAVV
                     LSGDPVTITITGIAPGERVRVEAVQEVRNATLRSYAVFVADGQGRVLPDVHAPVDGTY
                     GGVDPQGLFWSLAPGPGEHPDVFSRTTAPSFITISARTESGVNLSRVLERRLMGDGVT
                     RVPVNEAGVRGTLFLPNGSDPHPALIYLGGSEGGVNEGIAAIFASKGYVTLALAYFGV
                     PGLPQELVGIPVENVGDAVRFLNHHPRVKEDHIAIYGASKGAELALLSATRYPEIRAV
                     VANSPASVVFQGISLDWSAGPRSSWSENGSDLPYVPFIWYGDDDPILVSMGEAAQNGT
                     PWGTLAMYERALGDEAAVSNATIPVERINGPVLLLSAGKDTVWPSSRMSGDIMARLAE
                     YRHPFPDMRLDYPGSGHMIRTPWQSTELNRIFLP"
     gene            1414..>1897
                     /locus_tag="PKL26_07300"
     CDS             1414..>1897
                     /locus_tag="PKL26_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNS83537.1"
                     /translation="MKPVFTIAGLFILLLVVSGYAAADGEAWKFRSDLSNSGVYDDGG
                     MRPDGMTVWNYTTENEVYSSPTVVDGVVYIGSDDHSLYAFNAVTGDLIWSYMAGREIR
                     SSPAVADGVVYFGSDDYNIYALDAGTGDLIWNFITEQVARSSPVVADGVVYVAEGPHK
                     I"
BASE COUNT          368 a          540 c          626 g          363 t
ORIGIN      
        1 cggcaggaaa atcctgttca gctcggtcga ctgccatggc gtccgtatca tatgccccga
       61 ccctgggtag tcgaggcgca tatccgggaa cgggtggcgg tattcggcga gcctggccat
      121 gatgtccccg ctcatccgcg atgacggcca gaccgtgtcc ttcccggccg agagaaggag
      181 gaccggcccg ttgatccgct ccaccggaat cgtcgcgttg ctcaccgcgg cctcgtcgcc
      241 cagcgcccgc tcatacatcg cgagggtacc ccagggggtc ccgttctgtg ccgcttctcc
      301 catcgagacg aggatggggt catcgtcccc gtaccatatg aacgggacat aggggaggtc
      361 gctcccgttc tcggaccacg aggagcgggg gccggcactc cagtccagcg agatcccctg
      421 gaagacgacg cttgcagggc tgtttgcgac caccgcacgg atttccgggt agcgggtggc
      481 ggagaggagg gcgagctcgg cccccttgga agcgccgtag atcgcgatat ggtcctcctt
      541 cacgcgaggg tggtggttca ggaaccggac cgcgtctccg acattctcga ccgggattcc
      601 caccagttcc tgcggaagac cggggacccc gaagtaggcg agggcgaggg tgacatatcc
      661 tttcgacgcg aagatggccg cgattccttc gtttactcct ccctcagagc ccccgaggta
      721 aatgagtgcc gggtggggat ccgaaccgtt tggcaggaag agggttccac gcaccccggc
      781 ctcgttgact ggaacccggg tgaccccgtc acccatcagc cggcgttcca ggacccggga
      841 gaggttcact ccggattcgg tgcgtgccga gatcgtgatg aaggaggggg cggtggtccg
      901 cgagaagacg tccgggtgct cgccgggacc cggggccagt gaccagaaga gcccctgggg
      961 atcgaccccg ccataggtcc cgtccaccgg ggcatggaca tcggggagga ccctgccctg
     1021 cccgtcagca acaaagaccg cgtaagagcg gagcgtcgcg ttcctgacct cctggactgc
     1081 ctcgacccgg acccgctcgc caggagcaat gccggttatc gtgatcgtca ccgggtctcc
     1141 ggacaggacg actgcggggg tgaccgagag tgagggtgcc tgcggggtgg cgatgcatcc
     1201 tgcggcaacg agaaagacca cgagtatcaa tacggccgtc ccggagagca gcacagggca
     1261 ggtgaagcat gatggcatat tctcgtgttt tcatggtaga ggggataaag gtgtacgatc
     1321 tgggggttca ggcgtgccct cttgccacgg gatgcgcaca tacataaata gtgtccgcca
     1381 attgttcttt tacgacatat cggtagtgat cccatgaaac cagttttcac aatagcagga
     1441 ctgttcattc ttctccttgt ggtatccgga tacgcggcag ccgacgggga ggcctggaag
     1501 ttccggtccg acctctccaa ctccggagtc tatgacgatg gggggatgag gcctgacggg
     1561 atgacggtat ggaactacac gacagaaaat gaagtgtatt ccagcccaac agtggtagac
     1621 ggagtggtat atatcgggag cgatgaccac tccctctatg cgttcaatgc ggtcactgga
     1681 gatctcatct ggtcgtatat ggccgggcgg gaaataaggt cgagtcctgc ggtggcagat
     1741 ggtgtggtat attttgggag tgatgattac aatatctacg ccctcgacgc cgggaccggg
     1801 gacctcatct ggaattttat aacggagcaa gtggcacggt ccagtccggt cgtggctgat
     1861 ggggttgtct atgttgcgga gggtccgcac aagattc
//
LOCUS       DAOQVJ010000356         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_79337_length_1890_cov_4.178202, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000356 DAOQVJ010000000
VERSION     DAOQVJ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79337_length_1890_cov_4.178202"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKL26_07305"
     CDS             complement(<1..193)
                     /locus_tag="PKL26_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNS83538.1"
                     /translation="MHTPRPVQALLIDMDNTLFDLVGAKHAACRAITEKTGVGDADLL
                     FSYFLRRTRGFEDPGNILDF"
     gene            507..983
                     /locus_tag="PKL26_07310"
     CDS             507..983
                     /locus_tag="PKL26_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS83539.1"
                     /translation="MEIVKIPRMDKKEYDSLIEKGYVCRIAFQGEKYPYIAPFLYVFD
                     GKFLYFLSTKYGKKLEYFRKSPYVAVEVEKYTRDLSSYTFVSMQGYLEEVTDSIEKKL
                     IRQEFVDLIKERRLSKNILAALGHSPQDPPEAIAMEERSLVWKLTGVMDMVALKNL"
     gene            complement(1035..>1890)
                     /locus_tag="PKL26_07315"
     CDS             complement(1035..>1890)
                     /locus_tag="PKL26_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNS83540.1"
                     /translation="EMRTRPFCDEKVLADWNGLAIAALAMAGRASGKSRYIEAAEKAA
                     TFVLSRMRMDDGGLYHRFRDGSAGIHGLSADYAAMVWGILELFVATRDPGHLESALVL
                     EDYHDAHFWDKRSGGYFTSPESAIDLFARRKEFTDGAIPSPNSISFCNLVRIGLITQE
                     ERFGRRADALSRLYSPLVERSPASCGLFMAGFTLSAGPATRVVVTGKGTDPAFRGMNA
                     FLDRHYLPFTIPVSLEQGKQYELLFRLAPSIASHRFAGEAAVAHVCTMDSCAPPAYSV
                     EDLSLRLG"
BASE COUNT          448 a          522 c          520 g          400 t
ORIGIN      
        1 ggaagtccag tatattcccg gggtcctcga atccccttgt ccgccggaga aaatacgaga
       61 agagcagatc ggcatctcca actcctgtct tctcggtgat cgcacggcat gccgcatgct
      121 tcgcgccgac aaggtcaaag agggtattgt ccatgtcgat gagcagtgcc tggacaggac
      181 gtggtgtatg cattcctctc actgattaaa gaggtctctt ttgtccctta tggaagtatt
      241 gtttggcaac atgcgaaaca ggggagaaaa attttcctgt caaggagagg tatccgcttt
      301 tggcagcata caaatgcgca ggaataatct tatacagggc tgttcatctc ccattcgggc
      361 agccgccccc gccgtcctgc gtgccggccg cccatggccc cgaagtgccc cgttgtaaaa
      421 tcaaggcaat ccctggcatc tttttcgcaa acgcatgaat acacaaacta taagagtgag
      481 ggatcgtaat gctgaatgcg gtagttatgg agatagtgaa gatcccccgg atggataaaa
      541 aggagtatga ctccctgata gagaagggct atgtctgccg gatagccttc cagggggaga
      601 aataccctta tattgcacct tttctctacg tatttgacgg taagttcctc tatttcctct
      661 ccacaaagta tggaaagaag ctcgagtact tcaggaaaag cccttacgtc gcagttgagg
      721 tggagaagta cacccgcgac ctctccagtt atacctttgt cagcatgcag gggtacctcg
      781 aggaggtgac cgattcgatc gagaagaagc tgatccgcca ggagttcgtc gacctgataa
      841 aggaacggcg cctctccaaa aacatcctcg cggccctcgg gcattcgccc caggacccgc
      901 ccgaggcaat tgccatggag gagagatccc tcgtctggaa actgacaggc gtaatggaca
      961 tggtggccct gaagaacctc tgatcattca cctctccctt ttactgcctg ctgccgagat
     1021 cccaccacac ttcttcatcc aagtcgcaga gagaggtcct cgacagagta cgcaggtggt
     1081 gcgcatgagt ccatggtgca gacatgagcg actgccgctt cccctgcaaa gcgatgcgaa
     1141 gcgatggaag gggcgagtct aaacaggagt tcgtattgct tcccctgctc cagggaaacg
     1201 ggaatcgtga acggcaggta atggcggtca aggaacgcat tcatcccccg gaaggcggga
     1261 tctgtgccct ttcctgtcac taccacgcgg gtggcagggc cggcagagag cgtgaacccc
     1321 gccatgaaca agccgcatga tgcaggactc ctctcaacca ggggggagta aagacgggag
     1381 agtgcatcgg cacgccggcc aaaccgctct tcttgggtaa tgagcccaat gcgcacgagg
     1441 ttgcagaacg agatggagtt cggggaaggg atcgccccgt cggtgaactc cttcctcctt
     1501 gcaaagaggt cgatcgcgct ctcgggtgaa gtgaagtatc cgcccgatct tttgtcccag
     1561 aaatgggcat cgtggtagtc ctcgagcaca agcgcagatt ccaggtgccc tggatccctg
     1621 gtcgcgacaa acagttcgag gatgccccag accattgccg catagtcggc agaaagaccg
     1681 tgaatcccgg cggaaccatc cctgaaccgg tggtagaggc ctccgtcatc catacgcatc
     1741 cgcgagagga caaaggttgc agccttttct gctgcttcta tatatctgct cttgccagat
     1801 gcccttcccg ccatggcaag agcggcaatc gcaaggccgt tccagtcggc aaggaccttc
     1861 tcatcgcaaa aaggccttgt tcgcatctca
//
LOCUS       DAOQVJ010000357         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_79600_length_1886_cov_4.380666, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000357 DAOQVJ010000000
VERSION     DAOQVJ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79600_length_1886_cov_4.380666"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKL26_07320"
     CDS             complement(<1..184)
                     /locus_tag="PKL26_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83541.1"
                     /translation="MPVSEEIHPVAIANEFRQCRTCGYDRGFHTSLHRIAAGHPHFRV
                     VLICPECGTRYDARWVM"
     gene            complement(285..1178)
                     /gene="nifB"
                     /locus_tag="PKL26_07325"
     CDS             complement(285..1178)
                     /gene="nifB"
                     /locus_tag="PKL26_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNS83542.1"
                     /translation="MAENEYKVAEVAGKKVQWDPEQLRKIREHPCFSEKACHAFGRCH
                     VPVAPKCNIQCNYCIRDFDCVNESRPGVTSKVLNPQEAVTLVRDAIQKFPYIKVIGIA
                     GPGEPLANEETFETLRLLKEEFPNVIKCLSTNGLLLPDKIDLLHKYDVGNLTVTLNAI
                     DPEIGAKIYQFIDYKGKRYEGVEGAKVLLKNQLEGIRMAIERKMIVKVNTVYIPGINE
                     DHIPEIAKKVGEMGVYNFNVIPLIAQYRFSHITPPTPEMKVKMQDECAKYVRQMRHCQ
                     RCRADAVGRLGQDVQSCLYHE"
     gene            1397..1870
                     /locus_tag="PKL26_07330"
     CDS             1397..1870
                     /locus_tag="PKL26_07330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS83543.1"
                     /translation="MGSRMALAMSGMVLLLVIIAGCSTTSGVKAGDTIRVFYTVSFPG
                     TEPFEGNVNGTPLEFTVGDGTVIQGFENAVIGMNPGETKTVTISPEEGYGLRNETLVK
                     EVDIETALAFVQNLDQMGTSRILFFPGMEPVFEYTFPGSRGPLHLLQHYRGIHYY"
BASE COUNT          394 a          498 c          539 g          455 t
ORIGIN      
        1 ccattaccca ccgggcatca taccttgttc cgcattcggg gcatatcagc accactctga
       61 aatgggggtg cccggccgcg atgcgatgca gcgaggtatg gaatccgcgg tcgtacccgc
      121 atgttcggca ttgccggaac tcgttggcga tggcaacggg gtgtatctct tcagagacgg
      181 gcatagacgt cttcttccac gccagaggaa aaaaaggata ccccctgtcc cctgtgcagg
      241 ggcaggaagg atttttcaaa aaaaagggat ggggtggggg ccgttcactc gtgatagagg
      301 caggactgga cgtcctggcc aagccgtccg actgcatcgg ccctgcaacg ctggcagtgc
      361 ctcatctggc ggacgtattt tgcgcactcg tcctgcatct tcactttcat ctcgggtgtg
      421 ggtggcgtga tgtgggagaa ccggtactgg gcgatgagag ggatcacgtt gaaattgtac
      481 actcccatct ctccgacttt tttggcaatc tcggggatgt ggtcttcgtt gatgccgggg
      541 atgtagacgg tattgacttt cacgatcatc ttgcgctcta ttgccatccg gatcccctca
      601 agctggttct ttaagagtac ctttgcaccc tcgacgccct cgtagcgttt tcccttgtaa
      661 tcgatgaact ggtagatttt tgccccgatc tcgggatcga ttgcattgag ggtgacggtg
      721 aggttgccga cgtcgtactt gtggaggagg tcgatcttgt cagggagaag aaggccattg
      781 gtactcaggc acttgatgac gttcgggaac tcctccttca gcaggcggag cgtctcgaag
      841 gtttcttcat tcgcgagggg ctccccgggc ccggcaatcc cgatgacctt gatataggga
      901 aacttctgta ttgcatcgcg cacgagtgtg accgcctcct gcgggttgag gaccttggag
      961 gtcacgccgg ggcggctctc gttgacacag tcaaagtccc tgatgcaata gttgcactgg
     1021 atattgcatt tcggcgcgac cgggacatgg catcgcccga acgcgtggca tgccttctcc
     1081 gaaaaacagg gatgctcacg tatcttccga agctgctcgg ggtcccactg taccttcttc
     1141 cctgccacct ctgccacctt gtattcattc tctgccattt tgctcttctc ccgcaccgcg
     1201 cacacggcgc gcgtatgcta gagtgtgagg gttgtcatga taaatacaat tcgattcggg
     1261 aacgtaattc tcccgttttc ctgcaaaccg gagtgaataa agggggcgct cattcaggca
     1321 atcccgcagg gattattgcc gatcaaatgt atacggagag attatgagta gttccgatcc
     1381 tcgtcagaaa agattgatgg gaagccgcat ggcgcttgca atgtccggga tggtgttgct
     1441 gctcgtgatc atcgcagggt gcagcaccac ttccggggta aaggccggag ataccatccg
     1501 tgtcttttat actgtctcct ttcctggaac tgaaccattc gagggcaatg tgaacgggac
     1561 ccccctggag ttcactgttg gagacggcac ggtgatccag ggattcgaga acgcagtgat
     1621 cgggatgaac cccggcgaaa caaagacggt gacaatctct cccgaagagg ggtacggcct
     1681 caggaatgag acacttgtaa aagaggtgga tattgaaaca gcgttggcgt ttgtccagaa
     1741 cctcgaccag atggggacaa gccggatcct cttcttcccc gggatggagc ccgtctttga
     1801 atatacattt cccgggagtc gtggtccatt acacctttta caacattacc ggggaatcca
     1861 ctactattga ccagaaccac ccgctg
//
LOCUS       DAOQVJ010000358         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_79761_length_1884_cov_2.600328, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000358 DAOQVJ010000000
VERSION     DAOQVJ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79761_length_1884_cov_2.600328"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKL26_07335"
     CDS             <1..462
                     /locus_tag="PKL26_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS83544.1"
                     /translation="HDLGKVVVLAEPGASYSRSSGSTEEEVAMKLLKGEMEEVAPEHD
                     LEQSSEEYVANAVVCQGRLSELDRINETMVGTMEPVLDILLGKDLVTRQEERVVLTPL
                     AKVMAEHFIGVERLGEIRRLMRKIDDPLEIVAELECAEMEQKKEKEKKRRK"
     gene            467..940
                     /locus_tag="PKL26_07340"
     CDS             467..940
                     /locus_tag="PKL26_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS83545.1"
                     /translation="MLRPVLPALIAMLLVSACGCLAFTAQPAGSRGTDPVLPAPGVSD
                     TKMGARVIAMAVCADEIGTPYPEAADFLIKGLHCNANGGRFSEAIDYYDRALAIDPGC
                     STAWLAKGVALHNLGRYDEAILCYERASALDPGNEGIALLKEMAEGDRVEEHDTV"
     gene            1266..1475
                     /locus_tag="PKL26_07345"
     CDS             1266..1475
                     /locus_tag="PKL26_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83546.1"
                     /translation="MGLTRFPMGITLDRLHRNIEMGLQKSTSEPDACWIPIDPVGPIR
                     IPMSVAYIDGYYYDTPWIPHVYIPG"
BASE COUNT          474 a          468 c          523 g          419 t
ORIGIN      
        1 cacgacctcg ggaaggtggt cgtgctcgcc gagccaggcg cctcctactc ccggtcctca
       61 ggatcgacag aggaggaggt cgccatgaag ctcctcaagg gagagatgga agaggtcgcc
      121 cccgagcacg acctcgagca gagctcggag gagtacgtcg caaacgcggt tgtctgccag
      181 gggaggcttt ccgaactcga ccggatcaac gagacaatgg tcggcaccat ggagcctgtc
      241 ctcgatatac tgcttggaaa ggacctggtg acccggcagg aggaaagggt agtgctcacc
      301 ccgcttgcaa aggtgatggc cgaacacttc atcggggtgg agcgcctcgg cgagatccgg
      361 aggctgatga gaaagataga cgacccgctg gagatcgtgg cggaactcga gtgcgcggag
      421 atggagcaga agaaggagaa ggagaagaag aggaggaaat agtgaaatgt tgcgccccgt
      481 cctgcccgca ctcatcgcca tgctccttgt atcggcgtgc ggctgccttg cgttcaccgc
      541 acagcccgct gggtcacggg gaacagatcc ggtactcccg gcacctggag tatcggacac
      601 gaagatgggg gcacgggtca tcgcaatggc ggtatgcgcg gatgagatag ggactcctta
      661 tcccgaggct gcggatttcc ttatcaaagg cctgcactgc aacgcaaacg gaggccggtt
      721 ctcagaggcg atcgattact acgaccgggc tctcgccatc gacccgggat gttcgaccgc
      781 gtggttagcg aagggggtgg cgctccacaa cctgggtcgg tacgacgaag ccatcctgtg
      841 ttatgaaagg gcgtctgcgc tcgacccggg gaacgagggg attgcgcttt tgaaggagat
      901 ggcagaaggg gaccgggttg aagaacatga tacggtgtaa tgaacccggg agtgggtttt
      961 ttaaaaaacg gcgataatct ccacgaagga tcgaatttat atgtaatatt acgtataatt
     1021 tcacactgtt ggatatgatg tcccttaaga tgccggtttt tgtggtaacc attctatttc
     1081 tcacaacatc tcctgcggtt tcaggagcaa tgacatcact gtcattcctg caagattcac
     1141 gcagtctcct cttgtttcct tcatttgaaa aacccttcat tatatcccaa atctccaatc
     1201 cttcggtggg taagtttcaa gttgaaacta ttaagccggc atccctacag gtctcgcaat
     1261 tcaacatggg tctgacacgg ttcccaatgg ggatcaccct cgacaggttg cacaggaaca
     1321 ttgagatggg gcttcagaag agtacttcag agcccgacgc atgctggatt cccatcgatc
     1381 ctgtcgggcc gatacggatt cccatgtccg tcgcgtacat cgatggatat tattacgaca
     1441 ctccatggat accgcatgtc tacatacccg gataggctag tttcaggtat catgcaaatc
     1501 ctctggggca ctcccgccag gttcgggatc gcccctataa atgttaatga aaaatgatat
     1561 accgggtcca cttattatta cgaagaaata tgctcaataa accgacaata tcaattttta
     1621 tttgatattc aatgagagaa tcctcatgtt tctgctcata tcgtaaattc taatatactt
     1681 aacataccac catcattcct gacgtatgaa atggacaggg ttgggatgag aggagtgatg
     1741 caggtatgag aatgctgatg aacagcgggc ggacctacaa gcagggcgag caactttact
     1801 acaaggaaag cccggaatat tccgagcaga catctctctg ttttatcaac ccgatcgatc
     1861 tcttcacact cggaatcgaa gagg
//
LOCUS       DAOQVJ010000359         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_79994_length_1880_cov_4.402740, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000359 DAOQVJ010000000
VERSION     DAOQVJ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79994_length_1880_cov_4.402740"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1367)
                     /locus_tag="PKL26_07350"
     CDS             complement(30..1367)
                     /locus_tag="PKL26_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83547.1"
                     /translation="MQFHYALVDDLFSKEEFERRVEEKMEACGDLVDEVTAAMMVVQD
                     CGRHHVKIKDLSGRSSLFSFFGKVIAKNPPREFERPGGEKGLVTSLILGDETGQVRAI
                     LWDEKAMAAQEIEAGEVLEVIGRHPNRTPTEITVMAMRQAMVEIVGPTQVEPGLAPPK
                     RKEITARVMAVGEPRMVPRRDGTETEVLDLVVRSIEGIFRISCWVPDLIEGIEEGATL
                     RVQGVLEKVRKYGKEYSIDERSIIEIADEDIPQEIDSISDVKEGGIFSVCGSVRSCQP
                     ARSFTTRDGRPSCVRNLLLTDGSHEIRVVFWGEEHARTELLPEDRVTLYLCSARQGRS
                     GELELHAGSGSMVKVHHEGEAGEGEITGTVLATSGGTFLDTGAEHFLVRGHLPHGHEV
                     RARVTRRGKRISIHSFEEVLPDIKELNARIDAFLAMTDAGNTFTPHGRQDICR"
     gene            complement(1383..>1880)
                     /locus_tag="PKL26_07355"
     CDS             complement(1383..>1880)
                     /locus_tag="PKL26_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNS83548.1"
                     /translation="VPAVARGVEALGITPELWIEPGRFLVADSTILLTRVNSVKKAHK
                     NFVNVDAGFNLLVRPAMYDSYHEIVAAQSADRIPEETYTITGPICESGDILASDRRLP
                     LVRAGDLIAILDTGAYGFAMSSQYNSRPRCAEVIIHDGAPALMRRAETIEDIKATMRQ
                     PPWQS"
BASE COUNT          320 a          613 c          544 g          403 t
ORIGIN      
        1 tgttcacctg tcgtggagag aaagtacact cacctgcata tatcttggcg gccgtgcggg
       61 gtgaaagtat tccccgcgtc tgtcatcgcg aggaatgcat ctatcctggc attcagttcc
      121 ttaatgtcgg ggaggacttc ttcgaacgaa tggatggaga tccgcttccc cctcctcgtc
      181 accctggccc tgacttcgtg cccgtggggg aggtggccgc ggaccaggaa atgctctgca
      241 cccgtgtcaa gaaaggtccc tcccgaagtc gccaggaccg ttccggtgat ctctccttcc
      301 ccggcctcgc cctcgtggtg cacctttacc atgcttccgc tgccggcatg aagctcaagt
      361 tctcctgatc tcccctgccg ggcagaacag agatagagtg tcacccggtc ttccgggagg
      421 agctcggtac gggcatgctc ctctccccag aagacgaccc tgatctcgtg cgacccgtca
      481 gtgagcagga ggttgcggac gcatgagggc ctcccgtccc gggtggtaaa cgatcgtgcc
      541 ggctggcagg atcggacgga cccgcatact gagaatatgc cgccctcctt cacatcggag
      601 atagagtcga tctcctgggg gatatcctca tcagctatct cgatgatgct ccgttcatcg
      661 atgctgtact ccttgccgta cttccgcact ttctcgagga ctccctgaac ccggagagtc
      721 gccccctctt cgatcccctc tatgaggtca ggcacccagc atgagatgcg gaagatgccc
      781 tcaatgctgc ggaccacgag gtccagcact tccgtctcgg ttccgtccct tcgtggtacc
      841 atccggggtt ccccgaccgc catcacccgg gccgtgatct ccttcctctt tggaggggca
      901 aggccgggct cgacctgggt gggccccacg atctccacca ttgcctggcg catggccatg
      961 accgtgatct ccgtcggcgt ccggttgggg tggcgaccga tgacctcgag cacttctcct
     1021 gcttcgatct cctgggcggc catggccttc tcatcccaga ggatggccct cacctggcct
     1081 gtttcgtccc cgaggatcag gcttgtgaca agtcccttct ccccgccagg gcgctcgaat
     1141 tcccggggcg ggttctttgc aatcaccttt ccaaagaaag agaagaggct ggatctcccg
     1201 gagaggtcct ttatcttcac gtggtggcgc ccgcagtcct gcaccaccat catcgccgcg
     1261 gtcacctcgt cgacgaggtc cccgcaggcc tccatcttct cctccacccg ccgctcgaac
     1321 tcctccttcg agaagaggtc atcaacgaga gcgtagtgga actgcaccgg cccctctccc
     1381 cttcaggact gccatggagg ctgccgcatc gtggccttga tgtcctcgat tgtctctgcc
     1441 ctgcgcatca gggccggcgc gccgtcatgg atgatgacct ccgcgcaccg gggcctgctg
     1501 ttgtactgcg aggacatggc aaaaccgtat gccccagtgt ccaggatggc gatgagatct
     1561 cctgcacgga ccaggggaag acggcgatca gaggcaagga tgtcgcccga ttcgcagata
     1621 ggccctgtga tagtgtaggt ctcttcgggg atgcgatctg cgctctgtgc ggccacgatc
     1681 tcgtggtagg aatcgtacat ggccgggcgg acgaggaggt tgaaaccggc atccacgttg
     1741 acgaagttct tgtgggcctt cttgacggag ttcaccctcg tcaggaggat ggtcgagtct
     1801 gccacgagaa aacgcccggg ctctatccag agttccgggg tgatcccgag tgcctcgacc
     1861 cctcttgcga ccgccgggac
//
LOCUS       DAOQVJ010000360         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80412_length_1874_cov_5.398571, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000360 DAOQVJ010000000
VERSION     DAOQVJ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80412_length_1874_cov_5.398571"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKL26_07360"
     CDS             <1..330
                     /locus_tag="PKL26_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS83549.1"
                     /translation="PSKCVQCGTCIRSCPFHLLKSSGEHYLISVGGRRGRHPRVGREL
                     ITVNSPGAAADVVEKVVQWVYRRAWSGRLLSEQLDEIQFEKFREEIRSQIPASSVFEA
                     QDPDQSR"
     gene            complement(318..620)
                     /locus_tag="PKL26_07365"
     CDS             complement(318..620)
                     /locus_tag="PKL26_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83550.1"
                     /translation="MKTLPITKDASGNRATVECATGEVSVHKFCAFCEHCKGIKVGPR
                     VYPAPQEQVQKEMKRGSASDEALMVAALQFNQLVRDGTAIECADDQSQGFRPRYRL"
     gene            799..>1874
                     /gene="tuf"
                     /locus_tag="PKL26_07370"
     CDS             799..>1874
                     /gene="tuf"
                     /locus_tag="PKL26_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HNS83551.1"
                     /translation="MAAEKPHMNLAVIGHIDHGKSTTVGRLMFETGTVPAHIIEGYRK
                     EAEAKGKATFEFAWVMDNLKEERERGITIDIAHKRFDTPKFYFTVVDCPGHRDFVKNM
                     ITGASQADAAILVVAAPDGVMEQTKEHVFLARTLGIGQLIIAVNKMDAAKYDQKRFEE
                     VKKDLSALMQMVGYKPSETLFIPISSLAGANISKKSADTPWYTGPTLLEALDTLKEPE
                     KPVDKPLRLPIQDVYSISGIGTVPVGRVETGVMKKGMKVSFMPANKEGEIKSIEMHHE
                     EIPQALPGDNVGFNVRGIAKGDLRRGDVCGPVEAPPTVAEDFTAQIVVLQHPSAITVG
                     YTPVFHCHTTQTACTFIELKKKLDP"
BASE COUNT          422 a          570 c          527 g          355 t
ORIGIN      
        1 ccttcgaagt gcgtgcagtg cgggacgtgc atccgttcct gtcccttcca cctcctcaag
       61 tcgtccgggg aacactacct catctctgtg ggcggaagga gagggagaca cccacgggtg
      121 ggaagggaac ttatcacggt aaattcaccc ggtgcggctg ccgacgtcgt cgagaaggtg
      181 gtgcaatggg tataccgccg cgcatggagc gggaggctcc tctcagagca gctcgatgag
      241 atccagttcg agaagttccg ggaagagatc aggagccaga tccccgcctc atcggtattc
      301 gaggcgcaag atccagatca gagccggtaa cgcggcctga atccctggct ctgatcgtcc
      361 gcgcactcga tcgccgtgcc gtccctgaca agctggttga actggagggc ggcgaccatc
      421 agcgcctcgt cagatgcaga acccctcttc atctcctttt gcacctgctc ctggggggca
      481 ggatagacac gtgggccgac ctttataccc ttgcagtgct cgcagaatgc gcagaatttg
      541 tggacggata cctcccctgt ggcacattcg accgtggcgc ggttgccgct cgcatccttg
      601 gttattggca gggtcttcat gcttccggaa tgcattttat accgtcaccg atatgattct
      661 gtcgataata ccccggtttc cggcgaaatc gcccagaatc cggtcaatcg aaacgattaa
      721 tatggatccc tttgcaatat tttatactcg tataaatggg ctgaactctg acaggctcat
      781 ttttatcgga ggaatgacat ggcagctgaa aagcctcaca tgaatctggc ggtcatcgga
      841 cacatcgacc atgggaagtc caccacggtc ggaaggctta tgttcgagac cgggaccgtc
      901 cccgcgcaca tcatcgaggg ataccgcaag gaggccgaag ccaagggcaa ggccacattc
      961 gagttcgcat gggtcatgga caacctgaag gaagagcgtg agaggggtat caccatcgat
     1021 atcgcccaca agcgttttga cactcccaag ttctacttca ctgtcgtaga ctgccccgga
     1081 cacagggact ttgtgaagaa catgatcacc ggcgcttcgc aggccgatgc cgccatcctg
     1141 gtcgtagccg cacccgacgg ggtcatggag cagaccaagg agcacgtatt cctggcaagg
     1201 acgctcggta tcggtcagct tatcatcgcg gtaaacaaga tggacgccgc gaagtatgac
     1261 cagaagcgct tcgaagaggt gaagaaagac ctctccgcgc tgatgcagat ggtcggctac
     1321 aagccttccg agactctctt catccccatc agctccctcg caggtgcaaa catctccaag
     1381 aagagcgccg acaccccgtg gtataccggc ccgactctcc ttgaggcact cgatacgctg
     1441 aaggaacccg agaaaccggt ggacaagccc cttcgcctgc ccatccagga cgtatacagc
     1501 atcagcggca tcggcaccgt cccggtaggc cgcgtcgaga caggggtcat gaagaagggg
     1561 atgaaagtct ccttcatgcc ggccaacaag gagggcgaga tcaagtcaat cgagatgcac
     1621 cacgaggaga tcccgcaggc cctgcccggc gataacgtag gcttcaacgt gcgcggcatc
     1681 gccaagggcg acctccgccg cggtgacgtc tgcggccctg tcgaggctcc cccgaccgtc
     1741 gcagaggatt tcaccgccca gatcgtggtg ctccagcacc ccagcgccat caccgtggga
     1801 tacaccccgg tcttccactg ccacaccacc cagactgcct gcaccttcat cgagctgaag
     1861 aagaaactcg accc
//
LOCUS       DAOQVJ010000361         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80492_length_1873_cov_4.987899, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000361 DAOQVJ010000000
VERSION     DAOQVJ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80492_length_1873_cov_4.987899"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKL26_07375"
     CDS             complement(<1..832)
                     /locus_tag="PKL26_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNS83552.1"
                     /translation="MDILSAVLIIAGLILFETITSIDNAIINAEVLSTMKEWARRWFL
                     VWGLFIAVFVVRGVLPWLIVWMSTPALGPVGALTATFSSDPAVIAAIEESAPMLLMGG
                     GTFLVFLFFHWLFLEPKFYGIRGERYIATKGVWFFAVVSILLSLLVWEALQVNPMMAF
                     GAVVGSTAFFIVHGFRQNAEEQEKKMMGGNMSDISKIFYLEVIDATFSVDGVLGAFAF
                     TLSVPLIIIGNGIGAFVVRELTVRNVENIKKYRYLKNGAMYSIFVLGIIMILDSFGAH
                     I"
     gene            complement(997..>1873)
                     /locus_tag="PKL26_07380"
     CDS             complement(997..>1873)
                     /locus_tag="PKL26_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNS83553.1"
                     /translation="GMKIGMTVKGCDFMALHEYAKRKKIDMNNLLLIGVNCGGTVSPI
                     MARKMIREKFGVDPDTVHKEEIDKGQFIIEYEGGHKGIKIDDLEDEGYGRRTNCRRCV
                     YKVPRQADLACGNWGVIGDKAGKATFVEVCSEKGADLFSKAAKDGAISTQSPDPKGLE
                     MRGKVEGAMIKLGEKWRKKDFDALSKDLWGAIQKETSRCIKCYSCIENCPVCYPSADS
                     LKTKQYMVKPGEVPPNPMFHMRRFAHISDSCVNCGQCEELCAMDIPLAKFSHAIRVEA
                     DSAFEPKLGKSTYSN"
BASE COUNT          396 a          572 c          512 g          393 t
ORIGIN      
        1 ggatgtgcgc cccgaaactg tccaggatca tgatgatgcc aagaacaaag atggagtaca
       61 tcgcaccgtt cttgaggtag cggtatttct tgatattctc cacgttccgg accgtgagct
      121 ccctcaccac gaaggccccg atcccgttcc cgataataat gagcggcacc gagagggtga
      181 acgcgaatgc ccccagcacc ccgtcgaccg agaaggtggc atcgatcact tcgagataaa
      241 agatcttgct gatatcggac atgttcccgc ccatcatctt cttctcctgc tcctcggcgt
      301 tctggcggaa gccatggacg atgaagaatg cggtcgaacc cacgaccgcc ccgaacgcca
      361 tcatgggatt cacctggagt gcctcccaga ccaggagcga gaggaggatg gagaccacgg
      421 caaagaacca gacccccttt gtggcgatgt agcgctcccc ccggatcccg tagaacttcg
      481 gttccaggaa gagccagtgg aagaagagga agacgaggaa cgtcccacct cccatcagga
      541 gcatcggtgc cgactcctcg atggcagcaa tcaccgcggg atcgctgctg aacgtcgcgg
      601 tgagcgcccc cacgggtccg agcgcgggcg tggacatcca gacaataagc caggggagga
      661 cgccgcggac cacgaagacc gcgatgaaca gtccccagac caggaaccac cgcctcgccc
      721 actccttcat ggtggagagc acctcggcat tgatgatggc gttgtcaatg ctcgtgatgg
      781 tctcaaagag gatgagcccg gcgatgatca ggaccgcgga gaggatgtcc attggtgtat
      841 gcgacacgag cgttgtctac caatggtcaa atatcttacg tcatttccca gtaaaatcaa
      901 aaaaaatccc ggatatctgc aatgttcccg gcagcacatc accctgccgg ccttttcagt
      961 tccgatacta aaaaaccgaa aagaaaaatg ggggaatcag ttggaatatg ttgatttccc
     1021 cagtttcggc tcgaatgccg agtctgcctc gacccgtatt gcatgggaga acttcgcgag
     1081 cgggatgtcc attgcacaga gctcctcgca ctgcccgcaa ttgacgcacg agtcagagat
     1141 gtgggcgaac cggcgcatgt ggaacatggg gttcggcggg acttctccgg gctttaccat
     1201 gtactgcttg gtcttcagcg aatcggccga tgggtagcag accgggcagt tctcgatgca
     1261 cgagtagcac ttgatgcacc gggaggtctc tttctggatt gcaccccaga ggtcctttga
     1321 gagcgcatcg aagtccttct tacgccactt ctcgccgagc ttgatcatcg ccccctcaac
     1381 ttttcctcgc atctcgagcc ccttcgggtc aggcgactgg gtgctgatcg caccatcttt
     1441 tgctgccttc gagaagaggt ccgcaccctt ctcactgcag acctcgacga aggttgcctt
     1501 cccggccttg tcgccgatga caccccagtt cccgcacgca aggtctgcct ggcgcgggac
     1561 cttgtagacg cagcggcggc agttggtccg cctgccatac ccctcgtcct cgaggtcgtc
     1621 gatcttgatg cccttgtggc cgccctcgta ctcgatgatg aactggccct tgtcgatctc
     1681 ctccttgtgg acggtgtcgg ggtcaacgcc gaacttctcc ctgatcatct tccgggccat
     1741 gatcgggctc accgtgcctc cgcaattcac gccaatgagg aggaggttgt tcatgtcgat
     1801 cttcttccgc tttgcgtact cgtggagggc catgaaatcg caacccttca ccgtcatccc
     1861 gatcttcatt ccc
//
LOCUS       DAOQVJ010000362         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80502_length_1873_cov_3.449945, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000362 DAOQVJ010000000
VERSION     DAOQVJ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80502_length_1873_cov_3.449945"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKL26_07385"
     CDS             complement(<1..1182)
                     /locus_tag="PKL26_07385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNS83554.1"
                     /translation="MEDQLRNILFVFALQNAVKHAGLPKPGTVIGMVMGKHPELRSRA
                     REISALLDEVLSQVAALSPAEREQQLRALAPDVAKDLSRPKVQVKELPGLEKAEQGVV
                     MRFAPNPSGPLHLGHARAAVLNDAYVKRYGGKYILRIEDTDPKRVDPEAYGMVIRDID
                     WLGITVHEVVYQSDRFDRYYRYGKELIERGGAYVCTCENEQFRQLKQKKTACPCRSLS
                     VEENLTLFDKMLAGEFYEGEASVRVRTDLDHPDPAMRDFPAFRILHQPLHPRIDAVVY
                     PLMNFTVAVDDHLLGVTHVIRGKDHIANTRRQRYIFDYFGWDVPVYRHYGRMGIEGVV
                     LSTSQMRQGIASGEYEGWDDIRLGTLRALARRGISPEAVRQAVLDIGIGETDISFSWD
                     NL"
     gene            complement(1185..>1873)
                     /locus_tag="PKL26_07390"
     CDS             complement(1185..>1873)
                     /locus_tag="PKL26_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS83555.1"
                     /translation="GVPTVHTKWNMPTAILAGDVLFASAFEFIAMTDAPDNAKVRAIS
                     MLGRTCVQICEGQHMDMAFESRDDVLEGEYMKMVEKKTGVLYAASAGIGGILAGGNPS
                     YVDALYHFGLGIGMAFQIQDDIIDLVAPPEVSGKDRASDLREGKKTLIAMKALEKGFD
                     LSEFRRDLSPDEIDSVIKKLESLGVLEEVRITARDLIKNGKKRISILPDSEERQLLSD
                     LADYFLARSY"
BASE COUNT          424 a          539 c          459 g          451 t
ORIGIN      
        1 gaggttgtcc caggaaaatg agatgtctgt ctcccctatt cctatatcga ggacggcctg
       61 acggaccgct tcaggtgaga tccccctccg ggccagtgcc ctgagtgtgc cgagccggat
      121 gtcgtcccac ccctcatatt cacccgatgc aattccctgt ctcatctggg atgtggagag
      181 aacaaccccc tcaatcccca tcctcccata gtgacggtat accgggacat cccacccaaa
      241 ataatcgaaa atatatcttt gtcgccgtgt gttagcaata tgatccttgc ctcgaatcac
      301 atgcgttaca ccaaggagat ggtcatccac tgcaacagtg aagttcatca gaggatagac
      361 caccgcatcg attctgggat gaagtggctg gtgaaggatg cggaacgccg gaaaatcccg
      421 cattgccgga tccggatggt cgaggtcggt ccggactctc acagaagctt ctccttcgta
      481 gaactccccc gccagcatct tatcaaagag cgtaagattc tcctcaactg agagcgaacg
      541 gcagggacag gcagtcttct tctgtttcag ctgcctgaac tgctcgtttt cacaggtaca
      601 gacataggct cccccgcgct cgatcagttc ctttccgtac cggtaatagc gatcaaaacg
      661 atcgctctgg taaacgacct catggacggt gatcccaagc cagtcgatat ccctgatcac
      721 catcccatac gcttcgggat caaccctctt gggatcggta tcttcgatcc ggaggatgta
      781 ttttccgcca tatcgtttta catacgcatc gttgagaacg gcagctcgtg catgccccag
      841 gtgcagcggc ccgctgggat tcggagcaaa gcgcatcacg acaccttgtt ctgccttctc
      901 tagaccgggg agctccttta cctgtacttt gggccgggac aaatcttttg caacatcagg
      961 ggccagagca cggagctgct gctcccgttc ggcgggtgag agtgcagcaa cctgagagag
     1021 cacctcatcc aggagtgcag agatttccct cgcccttgag cgtaactcgg gatgcttccc
     1081 catgaccatc ccgatcacag tccccggctt gggaagacct gcatgtttta ccgcattctg
     1141 gagagcaaat acaaacagga tattccggag ttggtcttcc atgctcaata actccgggca
     1201 aggaaatagt cggccagatc tgagaggagt tgtctctctt ccgaatcggg gaggatgctg
     1261 atacgtttct tcccgttttt gataaggtca cgtgctgtta tcctgacctc ttcaagaacc
     1321 ccaagtgatt cgagtttttt tatgacggaa tctatctcgt caggggagag gtcgcggcgg
     1381 aattctgaca ggtcaaaccc tttctccagg gctttcatcg caataagggt ctttttccct
     1441 tcccggagat ctgaagcccg gtcctttcca ctcacttccg ggggcgcaac aagatcgatg
     1501 atatcatcct ggatctggaa ggccatcccg ataccaagcc cgaagtggta taaagcatcg
     1561 acatacgaag gatttccacc ggcgaggatt ccacctattc ctgctgatgc tgcatagagg
     1621 accccggtct tcttctccac catcttcatg tactcccctt ccagcacatc gtcccttgat
     1681 tcgaatgcca tatccatatg ctggccttca cagatctgca cgcaggtccg gccaagcatt
     1741 gagatagcac gtactttggc attgtcaggt gcgtcagtca tggcaataaa ttcaaaggcg
     1801 cttgcaaaga gcacatcccc ggccagaatt gcggtcggca tattccattt tgtatggacc
     1861 gtggggacac ctc
//
LOCUS       DAOQVJ010000363         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80553_length_1872_cov_4.468354, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000363 DAOQVJ010000000
VERSION     DAOQVJ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80553_length_1872_cov_4.468354"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PKL26_07395"
     CDS             <1..1169
                     /locus_tag="PKL26_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNS83556.1"
                     /translation="EEDGVWKEKKDQFIQFLKQEPGRYETWPATKAGPARDKDTLDKY
                     TYIPEIAALRDVRSQLDNLKWFRDEAWHELSEHVDTSENRLRAWLNPYGTQTARNAPP
                     ARVFLPAMSAWLRVIVSPGEGEVFEGSDWSSQEFAIAAVLSGDTNMMEAYKSGDPYVY
                     FAKVAGAIPQEAVNKWVKTPALAPEGEREKYAEFASTRDLFKATVLGLQYGMGAASLA
                     VKLTIDTGRKITESDAKKLISLHKRAFPRLWKWQDTIEHLYDREALVLNGGWALLQDN
                     PSALSVRNFPVQGTGSMMMHEALYLAQKRGIRMSFPLHDALYMIRGVQDTDAIKELEE
                     CMDEATNTILGDSIQIRHESVTVEPGKPWIEKKWAKKYEKYGRYLQNDLTPITE"
     gene            1539..>1872
                     /locus_tag="PKL26_07400"
     CDS             1539..>1872
                     /locus_tag="PKL26_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83557.1"
                     /translation="MAICEVAGLDPDAYTTEQDMENLFIDLASYTITLPLVMGPDRNP
                     QYLKAYVADKSTNAVPVRPDAAPAPAPRPLPQDRPVAPRQAPAAAVATPSPVPPHPAA
                     RPATPAPVA"
BASE COUNT          468 a          508 c          515 g          381 t
ORIGIN      
        1 tggaagagga cggggtgtgg aaagagaaga aggatcaatt tatccagttc ttgaagcagg
       61 aaccgggcag atatgaaacc tggccagcaa ccaaggccgg gcctgctcgg gataaggaca
      121 ccttggacaa atacacctac atcccggaga tcgccgcgtt gcgtgatgtg agatcccagc
      181 ttgacaatct caagtggttc agggatgaag catggcacga actctcggag catgtggaca
      241 ccagcgagaa tcggctacgg gcatggctga atccctacgg cacccagaca gctcgtaacg
      301 ccccaccagc tcgtgtgttc ctgccggcga tgtcagcctg gcttcgggtt attgtgagcc
      361 ccggtgaggg cgaggtgttt gaggggtcgg actggtcttc ccaggagttt gccattgccg
      421 ctgtgctctc tggcgatacg aacatgatgg aagcctataa atccggcgat ccgtatgttt
      481 acttcgccaa agtggctgga gcaatacccc aggaggctgt gaacaagtgg gtaaaaactc
      541 cggcacttgc tcctgaaggt gagagggaga aatatgctga gtttgcatcc acccgcgacc
      601 tgtttaaggc cacagttctg ggccttcaat atggcatggg tgctgcaagc ctcgcagtta
      661 agctcacaat cgacaccggg aggaagataa cggagtcgga tgcaaagaag ctaatcagcc
      721 tccacaagag ggcattcccc cgcctgtgga agtggcaaga caccatcgag catctttacg
      781 acagggaagc cctggttcta aacggtggat gggcactttt gcaggataat cccagcgcgt
      841 tgtccgtccg caatttcccg gtacagggaa caggctctat gatgatgcac gaagccctgt
      901 atctggccca gaaacgggga atccgcatgt ccttcccact acatgatgcg ctctatatga
      961 tccgtggtgt gcaggatacc gatgccatca aggagctaga ggagtgtatg gacgaggcta
     1021 ccaatacgat actgggggac tccatccaga ttcgccacga atcagtcacg gttgagcctg
     1081 gaaagccctg gattgaaaag aagtgggcca agaaatacga gaaatatggt agatatttac
     1141 aaaatgactt gactccaata acagaatagt ataattttgt aattgcaagt tgaattttgc
     1201 acggtaaacc ctacaaaaca caaaccaaag gagtgaatat gggttcttta gtattcggaa
     1261 acctgcgtga aacagcccat gcggctaaat ccaagatggt aggagcaaga atcccaccca
     1321 aaggtcgcta tgagtgcaag ctcgtcagcg tgaattttga gatcggaagc cggtcaccca
     1381 aaaagatggc tcggttcaag tggatggtgc tcaaaatcac ccaggtggct gatcctgtga
     1441 ccttggaagc cgcccaacag ttccgtggga aggaaatctc ccatgctttc gtcttggaag
     1501 gacctggatt cggctatgaa ggcttctctc ggatgatcgt ggccatctgt gaggttgccg
     1561 gcctcgatcc cgatgcctac accacggaac aggacatgga aaatctcttc atcgacctgg
     1621 ccagctacac tatcactctg cccctggtca tgggaccaga caggaatccc cagtacctga
     1681 aggcttatgt ggccgacaag tccaccaatg ctgtacctgt gcgccccgat gccgccccgg
     1741 ctcccgcccc gcgccctttg ccacaggacc gcccggtggc accacgccag gcacccgcag
     1801 cggctgtggc aacccccagc cctgttcctc cccaccccgc tgctcggcca gccacaccgg
     1861 cccctgtagc ac
//
LOCUS       DAOQVJ010000364         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80848_length_1868_cov_2.997242, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000364 DAOQVJ010000000
VERSION     DAOQVJ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80848_length_1868_cov_2.997242"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(462..>1868)
                     /locus_tag="PKL26_07405"
     CDS             complement(462..>1868)
                     /locus_tag="PKL26_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS83558.1"
                     /translation="NINPAYRTYELEYVLKQAEIQTVIVQGRFKTSDYTGMFREACPE
                     VNTSRPGHMETEKFPFLKNVIFMGDEPQQGMFSWSQIISRGQAVSKDELVAREESLDF
                     DDPINIMYTSGTTGFPKGVVLSHHSILNNGYIIGEGMNFSEKDRLCIPVPFYHCFGMV
                     LSNIACMTHGSTMVLPSPTFDALEVLKTVEKERCTALHGVPTMFIAELSHPDFPKYSM
                     KTLRTGIMAGSPCPIEVMKEVNRKMNMSEIVIVYGQTETAPGVTMTTTKDPLERRVST
                     VGRVFPHTEIKIIDPKSGKIVPRGEIGEICARGYCVMKCYYHNPSATNATLDENRWNH
                     TGDLGTIDEEGYVKIVGRLKDMVIRGGENIYPREIEEFLHHHPKIADVYVVGVPDVKY
                     GEELCAWVMVENGHSLSEEEVKEFCRGKIAHYKIPRYVMFVNEFPMSITGKIQKFKMR
                     EASIKALGLEDASKIETA"
BASE COUNT          362 a          557 c          526 g          423 t
ORIGIN      
        1 cgccccgcca ctgtggcatc ccctaagcgc gatagagcca gtgctgggta tctcgaagaa
       61 tcagaggttt ccagtatgct cttcttgcag actatcccgt atttgggggg ggtcggggag
      121 ggggattctc cccctccctg tcaaagaacc tagcgaactc aatttcccac ctcagtcaaa
      181 ccgcactcac cacacgatga cgctcctggg gctgataccc ccctatattt gcgcaccctc
      241 ctgatcacga tagggccagt tcctggagtg caatttcggc gaaatccatc acgttcaacc
      301 ctgcccgcag gccatcgcgt aaactggaga gtgtgcggga agaggtgaaa ttccttctcc
      361 actgtttcac ctgctgccgg ataaaaagaa aacagtaaat ctcagcggct gccataccac
      421 gtcggcggaa gaggaacgag gatcccgctc ttcatttacc ctcacgccgt ctcgatcttc
      481 gaggcgtctt cgagcccgag tgccttgatc gaggcctcgc gcatcttgaa cttctggatc
      541 ttcccggtga tgctcatcgg gaactcgttg acgaacatga cataacgggg gatcttgtag
      601 tgggcaatct tcccgcggca gaactccttg acctcctcct ctgaaagaga gtgcccgttc
      661 tcaaccatga cccaggcgca gagctcctcc ccgtacttga catccgggac gccgacgacg
      721 tacacgtccg cgatcttcgg gtggtggtgc aggaactcct cgatctctct cgggtagatg
      781 ttctcgccgc cacggatgac catgtccttt aagcggccca cgatcttcac atacccctcc
      841 tcgtcgattg tcccgaggtc cccggtatgg ttccagcggt tctcgtcgag cgtggcattg
      901 gtggcgctcg ggttgtggta atagcacttc atcacgcagt agccgcgtgc gcatatctcc
      961 ccgatctcgc cccggggcac gatcttcccg ctttttgggt cgatgatctt gatctcggtg
     1021 tgaggaaaga cacgtcccac cgtcgagacc cggcgttcca gcgggtcctt tgtcgtggtc
     1081 atcgtcacgc cgggggcggt ctcggtctgc ccgtagacaa tgacgatctc ggacatgttc
     1141 atcttccggt tgacctcctt cataacctct atcgggcagg gggatccggc catgattccg
     1201 gtgcgaagcg tcttcatcga gtacttcggg aagtcggggt gcgagagctc ggcgatgaac
     1261 atcgtgggga ccccgtgcag ggcggtgcag cgctctttct cgacggtctt tagcacctcc
     1321 agggcatcga atgtgggaga cggcaggacc atcgtgcttc cgtgggtcat gcaggcgatg
     1381 ttcgagagga ccatcccgaa gcagtgatag aacgggactg ggatgcagag ccggtccttc
     1441 tcggaaaagt tcatcccctc cccgataatg tagccgttgt tgaggatgct gtggtgagaa
     1501 aggacgaccc ccttgggaaa gcccgtggtc ccgcttgtat acatgatatt gatgggatca
     1561 tcgaagtcaa gcgactcctc ccgggccacg agctcatcct tgctcaccgc ctggccgcgg
     1621 gagatgatct gcgaccagga gaacatcccc tgctgcggtt catcgcccat gaagatgaca
     1681 ttcttcagga acggaaactt ctcggtctcc atgtgccccg ggcgcgaggt attgacttca
     1741 gggcatgcct cgcggaacat ccctgtatag tcagaggtct tgaaccgccc ctggacaatc
     1801 acggtctgga tctcggcctg cttgaggaca tattcgagct cgtaagtgcg gtaagccggg
     1861 ttgatgtt
//
LOCUS       DAOQVJ010000365         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80926_length_1867_cov_2.723510, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000365 DAOQVJ010000000
VERSION     DAOQVJ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80926_length_1867_cov_2.723510"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKL26_07410"
     CDS             <1..444
                     /locus_tag="PKL26_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83559.1"
                     /translation="IADVTTFAAIVQSVIDTNPFGCTPCEVGGVTFDPVSKNREYYTA
                     RILYQDDDGKTVAQITARSGSVGGFNTSITEIMGDAELTAAMGGDPVRDTEHERYLCT
                     LRCHDPSGEVYYVTFARDQVRISSYADDVIVGLVEAWADTVPALA"
     gene            482..613
                     /locus_tag="PKL26_07415"
     CDS             482..613
                     /locus_tag="PKL26_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83560.1"
                     /translation="MEQSVMIVAIFVGAGLLAYVIVPGLIVIWDRVLDAVEERKGED"
     gene            complement(674..1006)
                     /locus_tag="PKL26_07420"
     CDS             complement(674..1006)
                     /locus_tag="PKL26_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83561.1"
                     /translation="MMRKTLALCLLLGFVLVPATVMAAGGGGQGRSGAQGGAIALLDQ
                     ADGTDAAAGQYQFCAQHGSNDVQKAGYGNMLRIRSCTGEGEMSQAKTMARNQTRLRDG
                     SCGNCPPR"
     gene            complement(1122..>1867)
                     /locus_tag="PKL26_07425"
     CDS             complement(1122..>1867)
                     /locus_tag="PKL26_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83562.1"
                     /translation="PNGPGTGAGRLPCGPAQTAEPLSGATGPSDPAPSPSASPRSRRD
                     GNDEESAPEPAGVSPAPFFFLPFLGFRRITGKNVLAHDLRGRVYRAIASHPGIDALSL
                     AQLLETNPSTLRYHLFTLLRAEKITAFSRPGVVRYYPNQGMYSPFFQCLLHSLWTDTR
                     REIIVLLWHTPGMTRTQLAVALACAGPSVTRQMQGLAEDGIVENRGSGRSNHYHLTAA
                     AVEALGILGPMAGSLVLPALAAPALPSAA"
BASE COUNT          341 a          593 c          604 g          329 t
ORIGIN      
        1 attgcagacg tgaccacctt tgccgcgatc gtgcagagcg tcattgacac caacccgttc
       61 gggtgcaccc cctgcgaggt gggaggagtg acattcgacc cggtgtccaa gaaccgcgag
      121 tattataccg cacggatcct gtaccaggac gatgacggga agacagtcgc acagatcacc
      181 gcccgctcgg gaagcgtggg cggtttcaac acgagtatca ccgagatcat gggagatgcg
      241 gaactcaccg cggccatggg aggcgacccg gtcagagaca ccgaacacga gcgctacctc
      301 tgcaccctgc ggtgccacga cccctcggga gaggtctact acgtcacctt cgcccgcgac
      361 caggtgcgga tctcctcata cgctgacgac gtcatcgtgg ggctggtgga ggcatgggcc
      421 gacaccgtcc ctgcgctggc ctgattgcca cgcgatatgg accggtctca tggagacaac
      481 catggaacag tccgtcatga tcgtggcaat ctttgtaggg gccgggctcc ttgcctacgt
      541 gatcgtgcca ggcctgatcg taatctggga ccgggtgctg gacgccgtgg aagagagaaa
      601 aggagaggac taaaaatcct cccactctcc cccttcaccg gtaaaaaaat tcgtaatttt
      661 ttcacacttc caatcagcgc ggggggcagt ttccgcagga cccatcccgg agccgtgtct
      721 ggttccgcgc catggtcttt gcctgcgaca tctccccttc gccggtacat gagcggatgc
      781 ggagcatatt cccgtatcct gccttctgaa cgtcatttga cccgtgctgc gcgcagaact
      841 ggtactggcc tgctgctgca tcggtcccgt ctgcctggtc caggagcgcg atcgctcctc
      901 cctgtgcgcc ggacctcccc tggccgcctc cccctgccgc cataaccgtg gcaggcacga
      961 ggacgaagcc gaggaggagg cagagcgcaa gtgtctttct catcattgtt acctccgttg
     1021 ccgggtcgcc ggcaccgggc cgggcgaccc gcctcacaag aacatggggc aggggtattc
     1081 aagggatctc tcgcacgggc agctactcat caggagaacg gtcacgcggc ggaaggcaac
     1141 gccggcgcgg ccagtgcagg gagcaccagg cttcctgcca tcggcccgag aatcccgagt
     1201 gcctcgaccg cggccgcggt gagatgatag tggttcgacc tgcccgagcc ccggttctcc
     1261 acgatcccgt cctcggcgag cccctgcatc tgccgggtga ccgacggccc cgcgcaggcg
     1321 agcgcaactg caagctgggt cctggtcatc ccgggtgtgt gccagaggag cacgatgatc
     1381 tccctcctgg tgtcggtcca gagggaatgg aggaggcact ggaagaacgg ggagtacatc
     1441 ccctggttcg ggtaataccg cactacgccg ggccgggaga acgccgtgat cttctctgcc
     1501 cgcagcaatg tgaagaggtg gtaccggagc gtgctcgggt tggtctccag gagctgtgca
     1561 agggagaggg catcgatccc gggatgcgac gcgatggccc ggtacaccct cccccgcagg
     1621 tcatgtgcga ggacattctt cccggttatc ctcctgaagc caaggaatgg aaggaagaag
     1681 aatggggcgg gcgaaacccc ggcgggttcc ggggcggatt cctcgtcgtt tccgtctctc
     1741 ctcgatctcg gggaggcgga gggggacggg gccgggtcgg acggccccgt cgccccggaa
     1801 agcggctccg cagtctgtgc agggccgcag gggagccgtc ccgcgcccgt ccccggtcca
     1861 ttgggct
//
LOCUS       DAOQVJ010000366         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_80973_length_1866_cov_4.033131, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000366 DAOQVJ010000000
VERSION     DAOQVJ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80973_length_1866_cov_4.033131"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKL26_07430"
     CDS             <1..878
                     /locus_tag="PKL26_07430"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNS83563.1"
                     /translation="LYIADLSKKAAFEKAGLPVAGSVDDMLKAADIVVDATPGGVGEK
                     NRPLYEKVGIKAIWQGGEDHEVAGFSFNSDCNYRDAIGKQFARVVSCNTTGLCRIINA
                     MDKAYGVEKVRAVMVRRGGDPADIKRGPIDAVVLNPVHIPSHHGPDVQSVLPHINIVT
                     LAMIVPVTMMHMHVVQMDLKKEASREKVLEIIEKHPRMGLVRKSTGITSTAELKEYAM
                     DLGRCRSDLWENGIFEESVSCIGKELYLFQAIHQEADVVVENIDCIRAMMGTEKDPAK
                     SIATTNKAMNFVAIN"
     gene            1203..>1866
                     /locus_tag="PKL26_07435"
     CDS             1203..>1866
                     /locus_tag="PKL26_07435"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNS83564.1"
                     /translation="MDAYALATRNTVEVVTDEELREVLARPKKRVYAGYEPSGEIHLG
                     HLVTVNKLVDLKHAGFDVVVLLADLHAFLNRKGNMEQVRELAMYNRRCFEGLGLTDVE
                     YVLGSDLQLDRDYELMVLRLSQQITLNRATRSMDEVGRQMETPTVSQMIYPIMQMVDI
                     ARLGVDAAVGGIDQRKIHMLAREHLPSVGYPSPVCIHTPILNGLDGKKMSSSSGNYIS
                     VAD"
BASE COUNT          443 a          525 c          553 g          345 t
ORIGIN      
        1 ccctctacat cgcagatctc tcgaagaagg ctgcattcga gaaggccggc ctccctgttg
       61 caggcagcgt ggatgacatg ctgaaggccg cggacattgt ggtggatgca acgccgggcg
      121 gcgtggggga gaagaacagg ccgctctacg agaaggtggg catcaaggcc atctggcagg
      181 gaggcgaaga tcacgaggtg gcagggttct ccttcaactc ggactgcaac tacagggatg
      241 ccatcggcaa gcagttcgcc cgggtggtct cctgcaacac gacgggcctc tgccggataa
      301 taaatgccat ggacaaagcc tacggcgtcg agaaggtgcg ggcggtgatg gtgaggagag
      361 gcggggaccc tgctgacata aagcgcgggc caatcgatgc ggtcgtgctg aaccccgtgc
      421 acatcccgag ccaccacggc ccggacgtgc agagcgtgct gccccacatc aacatcgtca
      481 ccctggcaat gatagtcccg gtgacaatga tgcacatgca cgtcgtgcag atggacctta
      541 aaaaagaggc gagccgggag aaagtcctcg agatcatcga gaagcatccg aggatgggac
      601 tggtccggaa atccactggg atcaccagca ccgccgagct gaaggagtac gcgatggacc
      661 tggggcggtg ccgttcggac ctctgggaga acggcatctt cgaggaatct gtatcctgca
      721 tcgggaagga gctctatctc ttccaggcca tccaccagga agcggacgtg gtggtggaga
      781 atatcgactg catccgggca atgatgggca ccgagaaaga ccctgcaaaa tccatcgcca
      841 cgaccaacaa ggcaatgaac ttcgtggcaa tcaattaacc cctattcgat tttttgggct
      901 ggaaacattc tttttcgggt gttttccagc gtatgcctaa agagagccct cttatctccc
      961 ctctcctggc cagccctccc aaaaccagga gatgccagga aaatccgctg agtgggtatt
     1021 gtggcatccc atggatggca ccagaccctg gtgtgagaat aaagtgagtt gccatgaaac
     1081 ctggaccatc aaaaaaatca gacctttccc aatgaaaagc gcaacaccca cacagtatta
     1141 ctgaccggcc gcatctgtgc tgttcataag cattaacacc acgaattccc cattttatat
     1201 gaatggacgc gtacgcactg gcaacgagga acaccgtgga ggtggtcacg gacgaggaac
     1261 tccgggaagt cctcgccagg ccgaagaaga gggtgtatgc cggctacgag ccgagcggag
     1321 agattcacct cggccacctg gtcaccgtca acaagctcgt agacctgaaa cacgccggct
     1381 tcgatgtcgt ggtgctcctc gccgacctgc acgcctttct gaaccgcaag gggaacatgg
     1441 agcaggtccg ggagctggcc atgtacaacc gcaggtgctt cgaagggctc ggcctcactg
     1501 acgtggagta cgtgctgggg tcggacctcc agctcgaccg cgactacgaa ctgatggtgc
     1561 ttcgcctctc ccagcagatc acattgaaca gggccacgcg gagcatggac gaggtgggac
     1621 ggcagatgga gacccctact gtctcgcaga tgatctatcc catcatgcag atggtggata
     1681 tcgcacgact cggggtggat gcggcggtcg gcggaatcga ccagcgaaag atccacatgc
     1741 ttgcccggga gcacctcccg tccgtcgggt atccttcgcc tgtctgcatc cataccccca
     1801 tcctgaacgg gcttgatgga aagaagatgt cttcttccag tggaaattat atctccgttg
     1861 ccgaca
//
LOCUS       DAOQVJ010000367         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_81745_length_1855_cov_4.240000, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000367 DAOQVJ010000000
VERSION     DAOQVJ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81745_length_1855_cov_4.240000"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKL26_07440"
     CDS             complement(<1..818)
                     /locus_tag="PKL26_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS83565.1"
                     /translation="MSLCDDARSAAREILSHDEVTVISHIDADGIAGEAILAEALSRA
                     GIIHRSVFVRQLEPMTMRQVPEDSTFKLFCDLGAGQQNLLEERGLRDDEVLIVDHHVS
                     QPAGRAYQQVNCLDHGFSKMSAAGVAFIIAMEMDPGNVDLSRLAVVGNVGDMMAREDC
                     GLVGPARDIALQGASAGCIEVVSRDLNCYGTSTRPLHVCLAYNDDPFIPGITNNMNGA
                     LRFLQRLGIELQTPEKRWRVWEELSPQDKRLVTSSLVQQIISCGERADRLFSES"
     gene            975..>1855
                     /locus_tag="PKL26_07445"
     CDS             975..>1855
                     /locus_tag="PKL26_07445"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNS83566.1"
                     /translation="MVLRECREFQETGIITPSRMRAVDENAMALGVSSLQLMESAGKA
                     LADVVRSLKASPVVMLCGKGNNGGDGMVAARHLQDLDVTVVYLDGQGWSPDSGHQLKI
                     LSSCSVELLPVRCPEDVVSLGNHFQGAGCIVDALLGTGSTGNPREPIRSLVEAALKTT
                     AVKVSADIPTPGFAPDHILAFHRPKVEGSLVVDIGIPLEAEITVGPGDLTVLQRKDPA
                     AHKGAGGEVLVVGGGPYQGAPYLAGLGALRAGADIVRIASPVFEPVPDLIYERLEGGR
                     IGARHLETLKNLAERADV"
BASE COUNT          371 a          541 c          576 g          367 t
ORIGIN      
        1 tagctctcgg agaaaagccg gtcggcccgc tctccacagg atatgatctg ttggacgagg
       61 gaactggtca ccaggcgctt atcctgggga gacagctcct cccatacccg ccagcgtttt
      121 tcgggcgtct ggagttcaat cccgagcctc tgcaggaacc ggagcgcgcc gttcatgttg
      181 tttgtgatac ccgggatgaa gggatcgtcg ttgtaggcga ggcacacgtg gagcgggcgg
      241 gtggaagtgc catagcagtt caggtcgcgg gacacgacct cgatacaccc tgctgacgcc
      301 ccttgtagcg cgatatccct ggccggcccc acaaggccgc aatcctccct tgccatcatg
      361 tcccccacgt tgcccaccac cgcgagccgg gagagatcga cgttgcctgg gtccatctcc
      421 atggcgatga tgaatgccac tcccgcggca ctcatcttgg aaaaaccatg gtcgagacag
      481 ttgacctgct gatacgctct ccctgccggc tgactgacat ggtggtccac gatcagcacc
      541 tcgtcatccc gcaaccccct ttcctcgagg aggttctgct gtccggcccc aaggtcgcag
      601 aagagcttga aagtggaatc ttccggcacc tggcgcatgg tcatgggctc gagttgcctg
      661 acaaacaccg accgatgaat gataccggcc ctcgagaggg cctccgcgag gatagcctcc
      721 cctgcgatgc cgtcggcgtc gatatgggat atgacggtca cttcgtcgtg ggagaggatc
      781 tcccgtgccg cagaccttgc gtcgtcgcag agactcattg ggatagtaat ggaatatcaa
      841 ggagaaaaag ggtagggata agacaggtgc aaatcgggca ggatctggaa gtttcgcgcc
      901 aaagggggat acttcatatc tccaacggac gatatcctgg tacaggacgc ttcatgtacc
      961 gggagatctg actgatggtc ttgcgagaat gccgggagtt ccaggagacg ggaatcatca
     1021 ccccttcgag aatgcgggcg gtcgatgaga acgccatggc gcttggagtt tcctcactgc
     1081 agttgatgga atctgcggga aaggccctgg cggacgttgt ccggtccctc aaggcatccc
     1141 cggtcgttat gctgtgcgga aaggggaata atggcggaga cggcatggtc gccgcacgcc
     1201 atctccagga cctcgatgtg accgtggtat acctggatgg gcagggctgg agccctgaca
     1261 gcgggcacca attgaagatc ctttcgtcgt gctcagtgga gttgctacct gtccggtgcc
     1321 ctgaagacgt cgtctcactt gggaaccatt tccagggagc ggggtgcatc gtcgatgcgc
     1381 tgcttggtac tggcagcaca gggaatccga gggagccgat caggtccctt gtggaggctg
     1441 cactaaaaac aactgccgtg aaagtgtctg ccgacattcc cacccccggg tttgcccctg
     1501 accacatcct ggccttccac cgccccaagg ttgaaggatc actcgttgtc gatatcggca
     1561 taccgctcga ggctgagatc actgtcggcc ctggagacct cactgtcctg caacggaagg
     1621 accctgctgc gcacaagggg gccggtgggg aggtgctcgt ggtcggcggc ggcccctacc
     1681 agggagcacc atatcttgcc gggctcgggg cgctccgggc tggtgccgat atcgtgagaa
     1741 tcgcctcgcc cgtctttgag cctgtcccgg atctcatcta cgagcggctg gaagggggaa
     1801 gaataggcgc ccgacacctt gaaaccctca agaatctcgc ggaaagggcc gacgt
//
LOCUS       DAOQVJ010000368         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_82534_length_1844_cov_4.086641, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000368 DAOQVJ010000000
VERSION     DAOQVJ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82534_length_1844_cov_4.086641"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PKL26_07450"
     CDS             <1..707
                     /locus_tag="PKL26_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNS83567.1"
                     /translation="IHVHAFSPDEVSHAATRSGLSTREVLLAMKAAGLGSLQGTAAEI
                     LVDRVREIICPAKVPTDEWVRIIREAHGLGIPTTATIMYGSCETPRDRIAHLSILRDI
                     QDGTGGFSELVPLSYLHQNTRLYVEGKAPPGATGREDLLLIAVSRLFLDNFKNIQVSW
                     GKLGVKLGQVALLAGANDLAGTMFSDDVSGDAGASGSDFLDPREMERMVADIGRVLRR
                     RLTDYRLADDQSPTQE"
     gene            complement(686..1627)
                     /locus_tag="PKL26_07455"
     CDS             complement(686..1627)
                     /locus_tag="PKL26_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS83568.1"
                     /translation="MGFTHLFGPVSSRRLERSLGVDLVPYKVCSFDCVYCEVGSTTMR
                     TVTRQDFFPPSEVEEELVRFFSGEPSLDFITLSGSGEPTLSLSLGRVVRFVKSRFPAY
                     PLAVLTNGSLLWDSDVQHEILPADVVLPTLCTVDEEIFQKIHRPAPGLTIKKIVEGLR
                     DFRKIYPGEIWLEVFLIPGINMDRDHLSALGDEIRRIHPDRVQLNTLDRPGTEAWVRP
                     ASREQLADAAYHLGLPGAESIEPVPYQRSQEQISADPASMIVEMISRRPCTVEDIALT
                     TGLHLQEVGKLLRALSRDPRLMTKREERGIFYSWVGD"
BASE COUNT          403 a          526 c          526 g          389 t
ORIGIN      
        1 agatccacgt ccatgcattc tctccagacg aggtgagcca tgctgccacc cgctccggcc
       61 tctccacacg cgaggtgctt cttgctatga aagcggcggg gctcggatct ctccagggta
      121 ccgccgcaga gatccttgtg gacagggtaa gagaaatcat ctgcccggca aaggttccga
      181 cggacgaatg ggtccggatc atacgtgagg cgcatggcct tggaattccc accaccgcga
      241 ctatcatgta cggctcctgc gagaccccca gggacaggat cgcccacctc tccatcctgc
      301 gtgatatcca ggacgggacc gggggatttt cggaactcgt cccgctctcc tatctccacc
      361 agaacacccg gctctacgtc gaggggaaag ctcctccagg tgcgacggga agggaggatc
      421 tcctcctcat tgcagtctcc cgcctcttcc ttgacaactt caaaaacatc caggtctcct
      481 gggggaagct tggggtgaaa cttggtcagg tggcactcct tgcgggtgca aacgatcttg
      541 ccgggaccat gttctcggac gatgtttcag gcgatgcagg agcctccggt tccgacttcc
      601 tggatccccg cgagatggag cggatggttg cggatatcgg gcgggtgttg cgtcgcaggc
      661 tcacagacta tcgcctggcg gacgatcagt caccgaccca ggaatagaag atcccccgct
      721 cctctctctt cgtcatcaaa cgaggatccc tggagagtgc tctcaggagt tttcctacct
      781 cctggagatg cagccccgtc gtcagggcaa tatcttctac agtgcagggg cggcgactga
      841 tcatctccac aatcatcgat gcagggtctg cggaaatttg ctcctgggaa cgttgatagg
      901 gcacaggctc gatggactcc gcaccgggaa ggccgagatg atatgccgca tcggccagct
      961 gctcgcggct cgccgggcgc acccatgctt ccgttccggg gcggtcgaga gtgttcagct
     1021 ggaccctgtc cggatggatc ctcctgatct catctccaag ggcactgagg tgatcccggt
     1081 ccatgttgat gcccgggatg aggaagacct caagccagat ctctcctggg taaatcttcc
     1141 ggaagtcccg gagtccttct acgatctttt tgatggtgag gccaggtgct ggccggtgaa
     1201 tcttctggaa tatctcctcg tcgacggtgc agagggtggg gagcaccacg tccgcgggaa
     1261 ggatctcgtg ctgcacatcg ctgtcccaga ggaggcttcc attggtgagg actgccaggg
     1321 gataggcagg gaacctggat tttacaaacc tgaccaccct tccgagggag agagacaatg
     1381 tgggttcacc tgaccccgag agggtgatga aatcaaggga cggctctcca gaaaaaaaac
     1441 gcacaagctc ctcttccact tcggatggag ggaaaaagtc ctgccgtgtg acggtcctca
     1501 tggtggtgga ccccacctcg cagtatacgc aatcaaagga acagaccttg tagggaacga
     1561 ggtccacccc aagagaccgt tccaggcggc gggacgagac cggcccgaag agatgggtaa
     1621 atcccattgg tatactactt aacgctccgg atttctttaa gtgttggata gtacatgctg
     1681 atggaaaaga cgattatttg ataaaatcac aatgttcata gtttttccac caagataatt
     1741 ccctgtcgat gaaagaaatt tgcacagtgc agtatcagat aatcggggtc attcactccc
     1801 cgttcaccga tcagaagcgc accccaatcc agagcgcatt ctcg
//
LOCUS       DAOQVJ010000369         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_82676_length_1842_cov_4.016788, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000369 DAOQVJ010000000
VERSION     DAOQVJ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82676_length_1842_cov_4.016788"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..560
                     /locus_tag="PKL26_07460"
     CDS             201..560
                     /locus_tag="PKL26_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNS83569.1"
                     /translation="MNKEPATALAELHKGSWNKYEYDEASGQSRLDRRLYSSLHYLAD
                     YGFIVGTLAEDGDYLDVLIVTYEPFFPGCLVPVQPVGVLDIRDEKGRDQKILAVPTAD
                     PRFHEVADLAEARRRRR"
     gene            570..1718
                     /locus_tag="PKL26_07465"
     CDS             570..1718
                     /locus_tag="PKL26_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS83570.1"
                     /translation="MFDADKALALVRRLDFPRPCGSPEEARAQEIISEEIHSLGLDPI
                     HQEFAGWWIEPERAVLDLPDRKIEIEPAIPLPFLAAFPWMERVGLEVEITGILTEGIA
                     GQPLPDAPLIALRDSFQPESAVVSWAAAQLLLFPPIPEFIPYALIADPFVPSAYVPPK
                     RVGALRQMVRRQATLSWRPRRCTRLFRNVVAQIQGTVLPHEVVVLGAHLDSFPGTVGS
                     SDNAAGCAVIVETLRWFRSHPPERTVRFVWFTGEELDRRGSKAFVSGLLAQPEKICLF
                     INSDGGFEAGTGPPWVRVSTESMKSWVRTWLRDDVAIEVRGSDGDEEAFQERQIPTFR
                     ATGHSRQSAHLPTDRPEGLDEDKLALIGRITVEAALRATQAASALPVV"
BASE COUNT          334 a          610 c          572 g          326 t
ORIGIN      
        1 cgccgtgccc acctgcacgg cacaccgccc tcacgccacc ggatctacac cacgccccgg
       61 gactctaccc tccggaggtt ccatgagtaa ccgcaattac gaggcaacgt tagcttcggt
      121 aacagttact tctgaggcat cacgacgcct ggcaagaggc aaggtgtggg ccacaccgcc
      181 gggataagat cctgtcagcc atgaacaagg aacccgccac cgcgctggct gagctccaca
      241 aggggagctg gaacaagtac gagtacgacg aggcgtcagg ccagtcacgg ctcgaccgca
      301 ggctctattc ctcgctccac tacctggccg actacgggtt catcgtgggc acgctcgccg
      361 aagatggaga ctacctcgac gtcctcatcg tgacctatga gcccttcttc ccggggtgcc
      421 tcgtccccgt ccagccggtg ggagtcctcg acatacgcga cgagaaggga cgcgaccaga
      481 agatcctcgc cgtgcccacg gcagatccgc ggttccacga ggtggccgac ctcgccgagg
      541 cccgccgccg caggcgctga ggggggaacg tgtttgacgc agacaaggca ctggccctcg
      601 tgcgccgcct cgatttccca cggccgtgcg ggtcaccgga agaagcaaga gcccaggaga
      661 tcatctccga ggagattcac tccctgggcc tcgaccccat ccaccaggag ttcgcggggt
      721 ggtggatcga gcctgagcgc gccgtcctag atctccccga tcggaaaatc gagatcgaac
      781 cagcgatccc acttcccttc ctggccgcct tcccgtggat ggagagggta gggctcgagg
      841 tggagatcac gggtatcctg accgagggca tcgccggcca accgctccct gatgccccgc
      901 tcatcgccct ccgcgacagc ttccagcccg agtccgcagt cgtgtcgtgg gcggccgcgc
      961 agctcctcct ctttcctccg attcctgagt tcatccccta cgccctgatt gcggacccgt
     1021 tcgtaccatc ggcctatgtg cccccgaagc gcgtcggagc gttgcgacag atggtgaggc
     1081 gtcaggccac gctttcgtgg aggccgagac gatgtacacg gctcttccga aatgtggtgg
     1141 cgcagatcca aggtacagtc cttccccacg aggtcgtggt cctcggggcc catctcgact
     1201 cgttcccggg aacggtaggt tcctccgata acgctgccgg atgtgcggtg atcgtcgaaa
     1261 ctctgcgctg gttcaggagc caccctccag agcgcaccgt tcgcttcgtc tggttcaccg
     1321 gggaggagtt ggatcggcgg ggaagcaagg cgttcgtaag tgggctcctt gctcagcccg
     1381 agaagatctg cctcttcatc aacagcgacg gaggattcga agccgggacc ggtcccccgt
     1441 gggtacgcgt ctccactgaa tcgatgaagt cgtgggtgag aacttggttg cgggacgatg
     1501 tcgcgatcga agtccggggt agcgatgggg atgaggaggc ttttcaggaa agacagatcc
     1561 ccacgttcag ggccacggga cactctcggc agtccgccca tctccccacc gaccgccccg
     1621 agggtctcga cgaggacaaa ctagccctga tcggacggat cacggtggaa gccgctctcc
     1681 gcgcgactca ggccgcaagt gctctccccg tcgtgtagtt cgacgaaggc tcgcgaaggc
     1741 tccgcctagg cttgtggtcg gatgacgtcg agatctccgg tggtacccag gcttgtccga
     1801 tctcccgata ccccgtcccc ggcgggggcg gtagggaggc gt
//
LOCUS       DAOQVJ010000370         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_82682_length_1842_cov_3.303302, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000370 DAOQVJ010000000
VERSION     DAOQVJ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82682_length_1842_cov_3.303302"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKL26_07470"
     CDS             complement(<1..397)
                     /locus_tag="PKL26_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83571.1"
                     /translation="MAERDREDRRRNNAFKALKRNLKGAGSAVISYSGGLDSSFLAAI
                     AGETIPGAVRCVILDSPLVPRRTIQEAKARAALLRIPCDVVPFPILDDPEFSRNPRNR
                     CYICKKKGSALLLEKAREYGTGQAMDGLNA"
     gene            489..1253
                     /locus_tag="PKL26_07475"
     CDS             489..1253
                     /locus_tag="PKL26_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNS83572.1"
                     /translation="MADMLWVSGLMLSERERERYTRQLPLIGEDGQERLRGASVCVAG
                     AGGLGSPVLLYLAAAGVGRIRLVDPDRVERNNLNRQILHSEADIGRFKVESAAEKLRA
                     LNPDIEVEALPAAVDSGNASQMARGMDLLLDALDNYEGRYILNSAAFERKIPLVHAAV
                     SGFHGQLTTVIPGKTACLQCAMPHPPEGEDTAIIGVTAGIVGLLQALEAIKILLRKGE
                     IAGNVLLVWDGLWCRLDQIPLSRDPDCMVCGTGDYR"
     gene            1250..1531
                     /locus_tag="PKL26_07480"
     CDS             1250..1531
                     /locus_tag="PKL26_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNS83573.1"
                     /translation="MMSVRVRAFARFRELFGDRLEVAVSSPAMIRDVVMALGQVNADG
                     LLELLDGEGRVKNSVIILVNRERITGKGREEHPVADGDEVAIYPPVAGG"
     gene            1548..>1842
                     /locus_tag="PKL26_07485"
     CDS             1548..>1842
                     /locus_tag="PKL26_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNS83574.1"
                     /translation="MIRITREDFDPAQLLDEARSNEHGALVSFLGMVRDDGIERMDLE
                     AYEEVAEEDLARIRDEAIARFGVSRVDIVHRVGMLAVGDNIVMIVVGAPHRKAA"
BASE COUNT          380 a          472 c          589 g          401 t
ORIGIN      
        1 atgcattcag gccgtccatt gcctgccctg ttccgtattc gcgtgccttt tccaggagga
       61 gagcagatcc ctttttttta cagatgtagc agcggtttct tgggttccgg gaaaactccg
      121 ggtcatcgag gatcgggaat ggtacaacat cacacgggat ccggagaagt gcggcccggg
      181 cttttgcttc ctggatcgtg cgcctcggga caaggggaga atcgaggatc acgcatcgta
      241 ccgctcccgg aatcgtctcc ccggcaatgg ctgccaggaa cgagctgtcc aggccgcctg
      301 aatatgagat gaccgcgctt ccggcaccct tgaggtttct tttgagtgcc ttgaatgcgt
      361 tgttccttct tcgatcctcc ctgtctctct ctgccactcc cctctccccg cgagcattga
      421 tgatgcggtt acgcaccatt atgtttcagg atgactatat agtctctaca gtgcccgcct
      481 gtactgaaat ggcggacatg ttgtgggtga gcggactgat gctttcggag agggagcgcg
      541 aacgatacac gcgccagttg cccctgatag gggaggatgg gcaggagcgc ctccgcggtg
      601 catcagtatg tgtcgcggga gcaggcggtc tcggctcacc tgtcctgctc tacctggctg
      661 ccgccggtgt cggccgtata cgacttgtcg atccagaccg ggtggagaga aataacctga
      721 acaggcagat cctgcactca gaggcggaca tcggcaggtt caaggtggaa tctgccgcgg
      781 aaaaacttcg tgcgcttaac cctgacatag aggtcgaggc cctgcccgct gcagtggatt
      841 ctgggaatgc atcccaaatg gcacgcggca tggacctgct cctggacgca cttgacaact
      901 acgagggacg ctatatcctg aacagcgccg cattcgaacg aaagatccca cttgttcatg
      961 cggcagtgag cgggttccat ggacagttga cgactgtcat ccccggaaag actgcatgcc
     1021 ttcagtgcgc gatgccccac cccccggaag gggaggatac tgcgatcatc ggggtcaccg
     1081 ctggcatcgt cggtctgctg caggctctgg aagcaatcaa gatcctgctc cgtaaaggag
     1141 agatcgcagg gaatgtcctg ctggtctggg atggcttatg gtgcaggctc gatcagatac
     1201 ccctctcaag ggaccccgat tgtatggtgt gtggaacggg tgattacaga tgatgagtgt
     1261 cagggtcagg gcttttgcac ggttcaggga gctgttcggt gaccgcctcg aggtggcggt
     1321 atcttcgcca gccatgataa gggatgtggt gatggccttg ggacaggtga atgcagacgg
     1381 tcttctggaa ctgttggatg gtgaaggcag ggtgaagaac tccgtcatca tccttgtgaa
     1441 cagggagagg atcaccggga aaggcaggga ggaacacccg gtcgcggatg gcgacgaagt
     1501 ggcgatatat cctcctgtcg ccggagggtg acggcagggc gcatgaaatg attcgaataa
     1561 caagggaaga tttcgacccg gcacaactcc ttgacgaggc tcgaagcaac gaacacgggg
     1621 ccctcgtcag tttccttggc atggtgagag atgacgggat tgagcggatg gatctcgaag
     1681 cctacgagga agtggcagag gaggatctgg cccggataag ggatgaggct attgcccggt
     1741 ttggcgtttc gcgtgtggat atcgttcaca gggtagggat gctcgctgtc ggtgacaaca
     1801 tcgttatgat cgtagtgggg gctcctcacc ggaaagcggc at
//
LOCUS       DAOQVJ010000371         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_83195_length_1835_cov_3.094944, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000371 DAOQVJ010000000
VERSION     DAOQVJ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83195_length_1835_cov_3.094944"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKL26_07490"
     CDS             <1..457
                     /locus_tag="PKL26_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83575.1"
                     /translation="LSVMLIASGCTSPSPAVTTTPVPTTGAPVATTPMVTTPAVPSWA
                     GTWNTTWLNTDGNLTVSVVTMTAAGQEVSGNYSYTYPDEGPFTGHLNGTAKGQTLAGM
                     YSESDDDAGYIIFELSGDGNSFSGRWVHAEDQTELENSTLFWNGVRIAP"
     gene            complement(465..1187)
                     /locus_tag="PKL26_07495"
     CDS             complement(465..1187)
                     /locus_tag="PKL26_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS83576.1"
                     /translation="MGISERRQREKEQRRTEIVDAAEQLFFSRRYEEVSMDDIAREVE
                     LNKATIYLYFESKEALFATIVLRGVRILEEKYRECMDTQVPGIEKVALMGRAYSRFSE
                     EYPEYLRLIHFYGSERFSRENPYTAEIGKGYGTCRGILQDAIREGIDDGTIRNDLNPF
                     LASMYCMISFMGILSLEPKWKLVIEDEGFSSEQFTHEFFRFITPALSSGGTPHTMDER
                     EGSGPLSATPEPARKKKRKKLE"
     gene            complement(1284..>1835)
                     /gene="nudC"
                     /locus_tag="PKL26_07500"
     CDS             complement(1284..>1835)
                     /gene="nudC"
                     /locus_tag="PKL26_07500"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNS83577.1"
                     /translation="AGGSGEQDLFALFQEHDNGRGYPGVDNAAAGCANTRPSTTERAR
                     ICTSCSRIVYPRVSPAIIVLLKKGEKVLLARSPRFTPGVYSILAGFNEPGENLEQTVH
                     REVGEEVGIAVKNIRYFGSEPWPFPDSLMIGFVADYDSGEIRIDPREIEEAGWFSREN
                     LPPFPSKTSISRALIEAWRRGEV"
BASE COUNT          377 a          558 c          489 g          411 t
ORIGIN      
        1 gctctccgtc atgctgatcg cgtccggctg cacgagtccc tctcctgcgg ttactacaac
       61 acctgttccc acgaccggag ctccggttgc gacaaccccc atggtgacaa ctccggctgt
      121 cccctcttgg gccgggacct ggaacaccac ctggctcaac accgatggca acctgaccgt
      181 ctcagtcgta accatgactg cggccgggca ggaagtctcc gggaactaca gttacaccta
      241 tcccgatgaa ggccccttca ccgggcacct gaacggaacc gctaagggac agacccttgc
      301 cgggatgtat tccgagagcg acgatgatgc cgggtatatc atcttcgaac tctcagggga
      361 tgggaactcg ttttccggcc ggtgggtcca cgccgaagac cagaccgagc ttgaaaattc
      421 aacactcttc tggaacggcg tcaggatagc cccctgatga aaaatcattc caattttttt
      481 cttttcttct tccgggccgg ttcgggggtg gccgacagtg gcccggatcc ttcacgctcg
      541 tccatggtat gaggtgttcc ccctgacgaa agggcggggg tgataaaccg gaagaattca
      601 tgcgtgaact gttcgctgga gaacccctca tcctcaatca cgagtttcca cttcggttcc
      661 agggagagga tccccatgaa ggagatcatg cagtacatcg aggccaggaa cggattgaga
      721 tcgttccgga tcgtcccgtc atcgattccc tcccggattg catcctgcaa aatccctcgg
      781 caggtgccgt atcctttgcc aatctctgcg gtatacgggt tctccctcga gaaccgttcg
      841 gacccgtaga agtggatgag tcggagatac tcagggtatt cttctgaaaa ccgggaatac
      901 gcccggccca tcaaagcaac tttttcaatc ccgggcacct gggtatccat gcattcccta
      961 tatttttctt caaggatccg gacgccacgg agcacgatag tcgcaaagag agcctccttg
     1021 ctctcgaaat agaggtagat ggttgccttg ttcagttcga cctcgcgggc gatatcgtcc
     1081 atggacacct cctcgtacct cctagagaag aagagttgtt cggcggcatc gacgatctca
     1141 gtccttcgct gttccttctc ccgctgtctt cgctcggaaa ttcccatgct cttacaccct
     1201 ctcaatccct ggtaatgaat cacccttcgt ttcttgcaga ctcctctttc tacctggaga
     1261 aaccggcagc atgagaaaga tgctcaaacc tccccccggc gccacgcctc gatcagtgcc
     1321 cgagagatgc tcgttttcga agggaacgga ggaaggttct cgcgggaaaa ccagccggcc
     1381 tcttcgatct cccgggggtc gatccggatc tctccgctgt cgtagtctgc gacaaagcca
     1441 atcatgagag agtcggggaa cggccagggc tcgctcccga aataccggat gtttttcacc
     1501 gcgatgccga cctcttcgcc aacttcgcgg tggacagtct gctcgaggtt ctcccctggt
     1561 tcgttgaatc ctgcgaggat cgaatagacc cctggcgtga agcgggggga tctggcgagc
     1621 aggacctttt cgcccttttt caggagcacg ataatggcag gggatacccg ggggtagaca
     1681 atgcggctgc aggatgtgca gatccgtgcc cgctctgtcg tggaagggcg ggtatttgca
     1741 catcctgcag ccgcattgtc tacccccggg tatcccctgc cattatcatg ctcctgaaaa
     1801 agggcgaaaa ggtcctgctc gccagatccc cccgc
//
LOCUS       DAOQVJ010000372         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_83790_length_1827_cov_3.069977, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000372 DAOQVJ010000000
VERSION     DAOQVJ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83790_length_1827_cov_3.069977"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKL26_07505"
     CDS             <1..1115
                     /locus_tag="PKL26_07505"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNS83578.1"
                     /translation="PGLIDMHVHLREPGYEYKEDIASGSRAAASGGVTTVVCMPNTDP
                     VNDNPSVTRYIVEKARHTAVIRVLPAGSITKGLKGADLSEMGLMKAAGIVAVTDDGKS
                     VEDSSVMRRAMEYAATFGLPVISHCQDRSLSASGVMNEGDLSSRLGLGGIPGIAEDIV
                     ISRDVLLAQYTGIPIHITHVSTASGLDIIRQAKQAGVRVSCDVTPHHLLLTEDRVRGY
                     DTNAKMYPPLRSREDMLALVEGIRSGVVDCIATDHAPHAKDEKDLDFDLAPFGVIGLQ
                     TLLPGVFRLHREYGIDFSRLLACVTCNPARILGIEGGTLSPGARGDVTLFDPEALWTL
                     TPESVLSKSTNSPFLGQEFAGKVIQTIVAGQSVYRI"
     gene            complement(1112..>1827)
                     /locus_tag="PKL26_07510"
     CDS             complement(1112..>1827)
                     /locus_tag="PKL26_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS83579.1"
                     /translation="HTGIIDSHLLIRRMEAQCLEQGTTILYRTTITALEKTHPGFVCT
                     VCGPGGRPYSFLSRVVINAAGLGSDTIASLAGISVLEEGYRIYPVKGEYFRVNPAKQG
                     LVRGLVYPMPEKNLMGLGVHATKDLSGSLRLGPNALPTETISYDVDPSHARTFYESAS
                     RMLPFLRLEDLSPDTAGIRPKIRKPGGPERDFIIAHEAGRGLPGMINLIGIESPGLTS
                     CLSIGRYVADMAREDGLLP"
BASE COUNT          370 a          539 c          558 g          360 t
ORIGIN      
        1 ttcccggcct cattgacatg cacgtccatt tgcgggagcc gggctatgaa tacaaggaag
       61 atatcgcctc cggaagccgt gccgcagctt ccgggggagt gaccacggtc gtgtgcatgc
      121 cgaacaccga cccggtgaac gacaatccct ccgtgacccg gtacatcgtc gaaaaggcca
      181 gacacacggc ggtgatccgc gtgcttcccg cagggtccat taccaaaggg ctgaaaggcg
      241 ccgacttgtc cgagatgggg ctcatgaagg ccgcaggcat cgtagcggtg accgatgacg
      301 gcaagagcgt ggaagactcc tcggtgatga gaagggccat ggagtacgcc gcaaccttcg
      361 ggctgccggt gatctctcac tgtcaggaca ggagcttgag cgcatcaggg gtgatgaacg
      421 agggggatct gtcttcccgt ctgggcctgg ggggcatacc cggcatagca gaagatatcg
      481 tcatctcccg ggatgtactc ctggcacaat ataccgggat tcccatccat atcacccatg
      541 tgtctacagc gtcgggcctc gacattatcc ggcaggcaaa acaagccggc gtaagggtga
      601 gctgtgatgt gacgccccat catctcctcc ttacggaaga ccgcgtgcgc ggctacgaca
      661 ccaacgccaa gatgtatcct cccctccgca gcagggagga catgctcgca ctcgtcgagg
      721 gcatcaggag cggagtcgtc gactgtatcg ccactgacca cgcaccgcac gcaaaggatg
      781 aaaaggactt ggacttcgat ctggctccgt tcggggtcat cggccttcag acgctccttc
      841 cgggcgtctt tcggctgcac agagaatacg gcatcgattt cagccggctc cttgcgtgcg
      901 tgacctgcaa cccggcccgc atcctgggca tcgaaggcgg gacactctct cccggggcaa
      961 gaggcgatgt gacgctcttc gacccggagg cgctgtggac gctcacccct gaaagcgtgc
     1021 tttcgaagtc cacgaattcc ccgtttctcg gccaggagtt cgccggtaag gtcatccaga
     1081 cgatcgttgc gggacagagc gtctaccgga tctagggaag cagaccgtct tctctcgcca
     1141 tgtccgccac atacctcccg atcgacaggc acgatgtaag accgggagac tcgattccga
     1201 tgaggttgat catccctgga agtccccgcc cggcctcatg agcgataatg aaatcccgtt
     1261 ccggcccgcc cggtttccgt attttcggac ggattccggc agtgtccggt gagaggtctt
     1321 ccagcctcag gaaggggagc atgcggctgg cgctctcgta gaatgttctt gcatgagaag
     1381 gatcgacatc gtaagagatg gtctccgtgg gcaaggcgtt gggtccgagt cgcagggatc
     1441 cggagagatc tttggtggca tgcacgccca gccccatgag gttcttttcc ggcataggat
     1501 agacgaggcc gcggaccagc ccctgttttg caggattgac ccggaagtac tcccctttca
     1561 ccggatagat gcgatagccc tcttccaaga cgctgatccc ggccagtgac gcgatagtgt
     1621 cggagcctaa gcctgcggca ttgatcacga cccgggagag gaaggagtag ggtcttccgc
     1681 cgggcccgca gaccgtgcag acaaagccgg ggtgcgtctt ttccagagcc gtgatcgttg
     1741 tgcggtagag aattgtcgtc ccctgctcga ggcactgtgc ttccatcctt ctgatgagca
     1801 gatgggaatc gatgatacct gtatgtg
//
LOCUS       DAOQVJ010000373         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_84185_length_1822_cov_4.895869, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000373 DAOQVJ010000000
VERSION     DAOQVJ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84185_length_1822_cov_4.895869"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="PKL26_07515"
     CDS             complement(<1..1484)
                     /locus_tag="PKL26_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS83580.1"
                     /translation="MTAGLLVLLLFLAQAVSAAPPPFARLTLNHSQTYRNMTVAELET
                     IDLMDLSLSRVTLHSTSGDWTDTKNLTLPSVNAIRRVFSRNTSTWHGNTLVILGALDS
                     TVSGSGMDTFTIFIQEKMPVVQCQVPGADGDLFLIKGNATARNYTFSTLFESAERMYI
                     LHTKGIAGIEKTPAGWNTYEVGSPWSFGYPPDLGKILLENNISLTNFVLSSSHANSMS
                     SLFPPIHGEYLLSGFQYHPPSPGKVTTYAAWPVIILDGDNRLELVGKTPPYHYNKRTG
                     GDLALTFDNTANIHNLSYVLIREGETYDADVHVNMTALEESGVAVGLDLILSGNPFLS
                     ILKDTGLGQSDLKKVVSYSIQAVGNSTPPHSSHFSKINITPGYGTSGYALHASQVTIP
                     FDDLQGLLPGNFSIFALGTDANNTVVALDQDRISIGNSPLANFTASPTSGPAPLPVQF
                     TDLSSDSPTAWNWTFGDGGSSGAQNPNHTYTVPGLYNVTLTASNA"
BASE COUNT          399 a          448 c          561 g          414 t
ORIGIN      
        1 gcgttcgatg cggtcagggt aacgttgtag aggcccggaa cggtataggt gtgattgggg
       61 ttctgtgcgc cagaggaacc cccatcgcca aaggtccagt tccatgccgt gggagaatcg
      121 ctggagaggt cagtgaactg gaccgggagg ggggccgggc ccgatgtggg ggaggcggta
      181 aagttcgcca gcggggaatt cccgatcgaa atccggtcct ggtcgagtgc gacgacggta
      241 ttgttcgcat cagtcccgag cgcgaatatg ctgaagttgc cggggagcag cccctggagg
      301 tcatcgaagg gaatagtcac ctggctcgca tggagcgcat acccggatgt gccatagcct
      361 ggcgtgatgt tgatctttga gaagtgggag gagtgcggag gcgtggaatt gccgactgcc
      421 tggatggaat aagagactac cttcttcagg tcgctttgac caagtcccgt atctttcaaa
      481 attgaaagga acgggttccc tgagaggatg aggtccaggc ccaccgcaac gccggactcc
      541 tcgagcgcag tcatgttcac gtgcacgtcg gcatcgtagg tctcgccttc cctgataagg
      601 acatagctga ggttgtggat attcgcggtg ttatcgaagg tgagggcaag gtcgcccccc
      661 gtcctcttgt tgtagtgata tgggggagtt ttgccgacca gttcgaggcg attgtctccg
      721 tcaaggatga tgacaggcca tgccgcgtag gtggtgacct ttccgggact gggaggatga
      781 tattggaatc cagagaggag atattcgccg tggatgggtg ggaacagcga tgacatgctg
      841 ttcgcgtggg aggaggagag tacaaagttg gtgagtgaaa tattattctc gagcagtatt
      901 tttccaagat ctggaggata cccaaaactc cagggcgatc cgacctcgta tgtattccat
      961 cctgcaggag tcttctcgat accagcgata cccttggtat gcaatatgta catccgttcc
     1021 gccgattcaa acagggtcga gaaggtatag ttcctcgcgg ttgcatttcc cttaatcagg
     1081 aaaaggtccc cgtccgcacc ggggacctgg cactggacga ccggcatctt ctcctggatg
     1141 aatatggtaa aggtgtccat cccgctgccg gacacagtcg agtcgagagc gccgagtatg
     1201 acaagcgtat ttccgtgcca ggtggaagtg ttgcgggaaa ataccctgcg aatggcattc
     1261 acagagggga gtgtcaggtt ctttgtgtcg gtccagtccc ctgacgttga gtggagcgtg
     1321 acccgggaga gggagaggtc catcaggtct atcgtctcca gttcagctac ggtcatgttc
     1381 ctgtacgtct gggaatggtt cagtgtgagc cgggcaaagg ggggcggtgc cgcgctcacg
     1441 gcctgggcaa gaaaaagcag cagcaccagg agtccggcag tcaaccataa tgccctccat
     1501 ctattcaatg cgccatgacg gcgattcgcg aaccttgcgt gccagggata tgatacctct
     1561 ttcccactct ccatatctcg ttttctcctc ccacccctgt atgcaccctt ggcatggtgc
     1621 acagctttat atatatggtc cttgtaccac cctgcattta aatgtatggg gaaaaaacgc
     1681 ctgttcctac gctggatttc aaatgcgggg aggcaatcag cgggcggtca tcatcaagga
     1741 attgtgcatg cccccgtgag gagaattcgt tttgaacccg gttcctgaca caccgaattg
     1801 ataataagaa ggcccacacc at
//
LOCUS       DAOQVJ010000374         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_84349_length_1820_cov_4.724646, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000374 DAOQVJ010000000
VERSION     DAOQVJ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84349_length_1820_cov_4.724646"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..679
                     /locus_tag="PKL26_07520"
     CDS             227..679
                     /locus_tag="PKL26_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83581.1"
                     /translation="MPTRWAIATQALTHLVMAADPQQCRDNPAYLMDELGEEIRGIAD
                     RYQMPREDAGGLVQTLGAISVILFGPTFETPYIEGFHEESVIRLSECAMFRPEREKKR
                     DPSHVNRVCTAYVMSAICALNPEYTIRISRARCRGDSFCEMIIEKKKP"
     gene            769..>1820
                     /locus_tag="PKL26_07525"
     CDS             769..>1820
                     /locus_tag="PKL26_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS83582.1"
                     /translation="MKKEREHINHPVPPWHALPIEQVYETLISDGRGLSREEAARRLV
                     QYGKNSLPAREAPGIAEIVLHQFKSPLIYILLAAGIISILIGDVKDAGFIFLVVIINA
                     AIGTAQEWKAEQSAMKLQSLLQVMSRVMRGGTWQMLPAEDLVIGDVVQLESGMRVPAD
                     LRLLHAANLAIDESLLTGESVAVEKAVGVRDENIPVSDRSNMAYAGSTVVTGRGCGIV
                     TGTATRTEVGMIAEAVSGTESAKPPLIIRMEAFSQKVGILIIAASIVMAIVALSRGTP
                     PIEVFFLAVALVVSAIPEGLPVAITVALSIAASRMAQRHVIVRKLAAVESLGSCTTIA
                     TDKTGTLTVNQQTARVL"
BASE COUNT          412 a          512 c          547 g          349 t
ORIGIN      
        1 tgattctctt cgagacacac gcatacgcat tcatgaaaat ggcgcagcca ttttcccagt
       61 aaaactatat gggggttcat cctcctcgcg aatggctgtt cccgtggaca ttccatcgga
      121 aaagtgcttt atattcaccg gacgatatcc ctgatgcgaa gagagattcc gtttatacta
      181 ttgagcagga gagaaagaga cgatggttcg tgtgaaggac atccctatgc ccaccaggtg
      241 ggcaatcgcc acccaggcgc tcacccacct ggtgatggcc gcagatccgc agcagtgcag
      301 ggataatccc gcgtatctga tggatgaact gggagaggag atacggggga tagcagaccg
      361 ttaccagatg ccgagggagg atgcaggagg gctcgtgcag acgctcgggg caatctcggt
      421 gatcctcttc ggccccacct tcgaaacccc atacatcgaa gggttccacg aggagtcggt
      481 gatacggctc tccgagtgtg cgatgtttcg tcccgaaagg gagaagaaga gggatccgtc
      541 acacgtgaac cgggtgtgca ccgcgtacgt gatgagcgca atatgtgccc tgaaccccga
      601 atataccatc aggatctcgc gtgcacggtg ccggggcgat tccttctgcg agatgatcat
      661 cgagaagaag aagccttgat tttctgccgg cacatggcag gaggacacat ccgggggacg
      721 aaccattcca ggatggcaac cgaacacgcg aaaattgggt gacattccat gaaaaaggaa
      781 cgagagcaca tcaaccaccc cgttcccccc tggcacgccc tccctatcga gcaggtatac
      841 gagacgctca tctcggacgg gcgcgggctc tcgcgggagg aagctgcccg gcggcttgtg
      901 cagtatggga agaattctct gcctgcccgg gaagcgccgg gaatcgctga gatcgtgctc
      961 caccagttca agagcccgct catctatatc ctccttgcgg cagggatcat ctcgatcctg
     1021 ataggggatg tcaaggatgc agggttcata ttcctggtgg tgatcatcaa tgccgccatt
     1081 ggcactgcac aggagtggaa ggccgagcag agtgcaatga aactccagag cctgctccag
     1141 gtgatgtccc gggtcatgag gggagggacc tggcagatgc ttccggcaga ggacctggtg
     1201 atcggggacg tcgtgcagct cgaatcaggg atgcgggtcc cggcagatct ccgactcctc
     1261 cacgcggcga acctcgccat tgacgaatcg ctccttacag gagaatcagt ggcagtcgaa
     1321 aaggcagttg gtgtgcgcga tgagaacatc cctgtgagcg accggtccaa catggcgtat
     1381 gccggcagca ccgtggtcac cggaagggga tgcgggatag tcaccggcac ggccaccagg
     1441 accgaggtag gcatgatcgc cgaggcagtt tcggggacag agtctgcaaa acctcccctg
     1501 atcatccgca tggaagcctt ctcccagaag gtggggattc tcatcatcgc cgcaagcatc
     1561 gtcatggcga tcgtagccct gtcccgcgga actccgccca tagaggtctt cttcctcgcg
     1621 gttgccctcg tggtgtcggc catccccgag gggttgccgg ttgctatcac tgtcgccctc
     1681 tcaatcgcag cctcgaggat ggcgcaaaga cacgtcatcg tcagaaagct tgctgccgtc
     1741 gagagcctcg gaagctgcac gacaattgcc actgacaaga ccgggacact gactgtaaac
     1801 cagcagactg cccgggttct
//
LOCUS       DAOQVJ010000375         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_84483_length_1818_cov_5.588202, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000375 DAOQVJ010000000
VERSION     DAOQVJ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84483_length_1818_cov_5.588202"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKL26_07530"
     CDS             <1..271
                     /locus_tag="PKL26_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNS83583.1"
                     /translation="LCNASRLPRLLPWKFDRVVMNLPLGAVRFLPAAMELCRPGGTIH
                     CYTLQEKEGEYLEEIGTHPVTSVRERVVRSYSPGRWHAVYDITLK"
     gene            complement(286..495)
                     /locus_tag="PKL26_07535"
     CDS             complement(286..495)
                     /locus_tag="PKL26_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNS83584.1"
                     /translation="MEISARNVFKGKVKSIKYGQILAELVIELPGGLEITSLITRTSA
                     DRMKLVVGKSISTIIKASNVMVATD"
     gene            complement(831..1226)
                     /locus_tag="PKL26_07540"
     CDS             complement(831..1226)
                     /locus_tag="PKL26_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNS83585.1"
                     /translation="MSSVARFWRKIPQRYNLIGTRCSTCGRYFFPPRSFCPDCRRAGN
                     IVRYKFPGTGSVVTYTIVRTASEQFEQMTPYALAIVQLDEGPRFTAQLVCSPEEVKIG
                     MRVRPVFRRISADGESGVIHYGTKYVPEG"
     gene            complement(1228..>1818)
                     /locus_tag="PKL26_07545"
     CDS             complement(1228..>1818)
                     /locus_tag="PKL26_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314556.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNS83586.1"
                     /translation="LDTVLKSTLVADPLRLFDCSPITDGAAAVVLAPLDMARKFCDTR
                     VKVLASAQASDTIALHDRREISTLDATVAAGRRAFAMAGLGPKDIDLVEVHDCFTIAE
                     ICAIEDLGFCAKGDAGRLTMEGVTALNGDIPVNTSGGLKACGHPVGATGIKQVCEVVM
                     QLRGQAGKRQVDGARIGMTHNVGGTGATVAVHILGV"
BASE COUNT          354 a          557 c          532 g          375 t
ORIGIN      
        1 tctctgcaat gcatcccgcc tcccccgcct ccttccctgg aagttcgaca gggtggtgat
       61 gaacctcccg ctcggggccg tcaggttcct tcccgccgcc atggaactct gcaggccggg
      121 cggaacgatc cactgctaca ccctgcagga gaaagagggg gagtatctcg aagagatcgg
      181 gacgcaccct gtcacatctg tccgggaacg ggtggtccgc tcctactccc cgggccggtg
      241 gcacgcggta tacgacatca cgctgaaatg agcctcgtca aatccctaat cggttgcaac
      301 catcacgttt gaggctttga taatcgttga aatgctcttc ccgacgacga gtttcatccg
      361 gtccgccgac gtcctggtaa tcagcgaggt gatctccagt ccccccggca gttctatgac
      421 cagctccgca aggatctggc cgtatttgat cgactttacc tttcccttga acacgtttcg
      481 tgcgcttatt tccatggtcg tcaccgtgtg ccaattgtaa agtacttttt atatattttg
      541 gcatggtgac agacatacgg ggatgtcctg gtatgtcatc tggtaaagag tgccgggaat
      601 ggaagaacca tgtcataacc gtgaagaaac ggtatgaaag aatgaacgat caggcaaaca
      661 gggtgtgatc cgttcgcact ccctggatta agaggggaag ttccccgtga atgagccaga
      721 ccgatcgcca tgccggagtt ttcactcaac cggttcgcca tggggatcgt tctctctcag
      781 gggtgatatg ctgagaagag gggcaaccag tacgaagaga aaagggattt ttagccttcc
      841 gggacatact tggtcccgta gtgaatgacg cccgattccc cgtcggccga gatgcggcgg
      901 aagacgggcc tgacacgcat cccgatcttc acctcttccg ggctgcagac aagctgtgcg
      961 gtgaacctcg gcccctcgtc gagctgtaca attgcaagag cgtatggcgt catctgctca
     1021 aactgctcgc ttgcggtgcg gacgatcgtg taggtcacca cgctgccggt accggggaac
     1081 ttgtatctga caatatttcc ggcacggcgg cagtcagggc agaaggaccg gggcgggaag
     1141 aagtatctcc cgcaagtgct gcaccgggtg ccgatcaggt tgtaccgctg ggggatcttc
     1201 ctccagaacc gcgcgacaga ggacatctca cacccccagg atatgcacgg cgacggtcgc
     1261 gcccgtgccc ccgacgttgt gcgtcatccc gatcctcgcc ccgtcgacct gccgcttccc
     1321 ggcctggcca cggagctgca tgaccacctc gcagacctgc ttgatcccgg tcgcaccgac
     1381 ggggtgcccg caggccttca gcccgccgct tgtattgaca gggatgtccc cgttgagtgc
     1441 cgtgaccccc tccatggtca gcctgccggc atctcccttg gcgcagaacc cgaggtcctc
     1501 gatcgcgcag atctcggcga tggtgaagca atcgtgcacc tctacgagat cgatatcctt
     1561 tggcccgagc ccggccatgg caaaggcgcg ccttccggct gcgacggtcg cgtcgagggt
     1621 gcttatttcc ctccggtcgt ggagggcgat ggtgtcgctc gcctgggcgc tcgcgagcac
     1681 cttcacccgg gtatcgcaga acttcctggc catgtccagc ggcgcgagga cgaccgccgc
     1741 cgcaccgtca gtaatcggag aacagtcgaa gagccgcagc ggatctgcga cgagcgtcga
     1801 tttcaggaca gtgtccag
//
LOCUS       DAOQVJ010000376         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_84484_length_1818_cov_5.374929, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000376 DAOQVJ010000000
VERSION     DAOQVJ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84484_length_1818_cov_5.374929"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="PKL26_07550"
     CDS             complement(<1..1484)
                     /locus_tag="PKL26_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS83587.1"
                     /translation="MRTDASEQDIPSQVDLCLRARITLLILHTAEEERAIRMLQDVCG
                     RFSPKRPFFTWDHSDGFAGPAGTPCRGSSARDPLVALEEMEKTDEASVIVLKDFHEFW
                     GNPGVKRRLRNLAQKFRFSRRTMVVVTPSLKVPEEIRDDAVVLHVPPPSARDLEHELD
                     RLLRGSGAGDSLTPLGREKLIQAALGMSLNQACRSFSKAIVRRGRLDDRDIDSVVSEK
                     KDILSQSEALEFYSATEYAENVGGLSVLKEWLRLRERAFSREAREYGLPSPKGIALIG
                     IPGTGKSLTAKMIAGMWRLPLLRLDVGALFGSFVGESEERTRRALALAETISPCILWI
                     DEMEKAFSFGNGDSGTSQRVFATILTWMQDKTSPCFVVATANDISALPPELLRKGRFD
                     EVFFLDLPSLGERREILSVHLRRRGLLPDDFDLDRLAAASEGYVGAEIEQTVIDAMYL
                     AFNQDTRQVTTSDILSALGAQVPLSISQKEVIGGLRAWLTEGRAL"
     gene            complement(1481..>1818)
                     /locus_tag="PKL26_07555"
     CDS             complement(1481..>1818)
                     /locus_tag="PKL26_07555"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNS83588.1"
                     /translation="LEEMGYQVETGGTIRGYHGVQQVDLSVTGRDGYGVGFVRNGDGC
                     YDLLADSWGVKGADKKILDSLRRNYPRIQQEYARRTVREQAEREGYTMVEEVEQEDGS
                     VRIVVRRWA"
BASE COUNT          320 a          603 c          548 g          347 t
ORIGIN      
        1 agtgcccgcc cctcggtcag ccacgcccga agtcccccga tcacttcctt ctgcgagatc
       61 gagagcggca cctgtgcccc cagcgcggag aggatgtcgc tggtggtgac ctgcctggta
      121 tcctggttga acgcaaggta catggcatcg atgaccgtct gctcgatctc ggcacccacg
      181 tatccctccg acgccgcggc caggcggtcc aggtcgaagt cgtcgggaag gagtccgcgc
      241 cgtcggaggt ggaccgataa tatctccctg cgctctccaa gcgagggaag gtcgaggaag
      301 aacacctcgt caaacctccc cttgcgaagg agctccgggg ggagcgcaga gatgtcgttt
      361 gccgtcgcca ccacgaagca gggggaggtc ttgtcctgca tccaggtgag gatggtcgca
      421 aagaccctct ggctggtccc ggagtcaccg ttcccgaacg aaaatgcctt ctccatctcg
      481 tcgatccaga ggatgcaggg cgagatggtc tctgcaaggg caagtgcacg gcgggtgcgc
      541 tcctcagact ccccgacaaa gctcccgaag agcgctccca cgtcgaggcg gagcagcggg
      601 agcctccaca tcccggcaat catctttgcc gtgaggctct ttccggtccc ggggatgcct
      661 atcagggcga tccccttcgg agacggcagc ccgtactccc gggcctcgcg cgagaatgcc
      721 cgctccctca ggcggagcca ctccttcagc acggaaagcc cgcctacgtt ctccgcgtac
      781 tctgtagcgc tgtagaactc gagcgcctcg ctctgggaga ggatgtcctt cttctcggag
      841 accactgaat cgatgtcgcg gtcatcgagg cgcccgcggc ggacaatcgc cttcgaaaac
      901 gacctgcagg cctggttgag ggacatcccg agggctgcct ggatcagctt ctcccgcccg
      961 aggggggtga gggaatctcc tgccccgctc ccgcgcagca gccggtcgag ttcgtgctcg
     1021 agatccctcg cagaaggcgg gggcacgtga agcacgaccg catcgtcacg gatctcctcg
     1081 ggcaccttca atgacggggt gaccaccacc atggtcctgc gggagaaccg gaacttctgg
     1141 gccaggttcc ggagccttcg cttcacgcca gggtttcccc agaactcatg gaagtccttg
     1201 agcacgatca cggaggcttc gtcggtcttc tccatctctt cgagggctac gaggggatct
     1261 ctcgcagaag agcccctgca gggtgtgccg gccggccccg caaacccgtc tgaatggtcc
     1321 caggtgaaaa aaggcctttt gggggagaag cggccgcaga catcctggag catcctgatc
     1381 gccctctcct cctcggcggt gtgcaggatg agcaaggtga tgcgtgccct caggcagagg
     1441 tcgacctggg aggggatatc ctgctcgctg gcatctgttc tcatgcccat ctccggacca
     1501 cgatgcggac cgagccgtct tcctgctcaa cctcttctac catggtatat ccttcacgtt
     1561 ctgcctgctc acgcaccgtc ctccgggcgt actcctgctg gatgcggggg tagttccggc
     1621 gcagggaatc gaggattttt ttgtctgcgc ctttcactcc ccaggagtct gccaggagat
     1681 cgtaacagcc gtcaccgttc cggacgaacc ccacgccgta tccgtctctt cctgtaacag
     1741 agaggtcaac ctgctgcacg ccgtggtaac ccctgatggt cccccctgtc tcgacctggt
     1801 agcccatctc ctcgaggc
//
LOCUS       DAOQVJ010000377         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_84726_length_1815_cov_5.348295, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000377 DAOQVJ010000000
VERSION     DAOQVJ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84726_length_1815_cov_5.348295"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKL26_07560"
     CDS             <1..227
                     /locus_tag="PKL26_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNS83589.1"
                     /translation="NAYGQVDMELLARAAGIRRTCKVQDEQELRDAWEDRGRGPTFIH
                     VVLKPGNATVPNIPLAPAGIRERFGRALRG"
     gene            complement(250..732)
                     /locus_tag="PKL26_07565"
     CDS             complement(250..732)
                     /locus_tag="PKL26_07565"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNS83590.1"
                     /translation="MDQLVWCGRIKNGISLTAPNETLASAYLKKAEEALETMQTITAR
                     DWKITTAYYAMYFSLYAILTRLGVRCENHSCTILLMERLLSDYFAPAEMALVERARGA
                     RVDAQYYVSREIPDPFCEELIRAAPRFLVKCTGIVNGMSERTVETLRKRLIEALREEG
                     "
     gene            complement(732..1316)
                     /locus_tag="PKL26_07570"
     CDS             complement(732..1316)
                     /locus_tag="PKL26_07570"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNS83591.1"
                     /translation="MSKKLDIREDHLRVLSLFTWGYDREVYIREVCRYLPISRGTAQT
                     ALEYLEGKQVLRSVTRGRTRLFSLQRGNHARDFCILAETYRKICFGEEEPFIAGVLGR
                     IIPLIEGCGAVFGSHAGGTADEDSDLDLFIAGECRRDEIERVGKTYGLPIHLTVYPLE
                     IFDRSLHTDPFLREVLRNHVLIKGTEHFVEQAVG"
BASE COUNT          359 a          527 c          541 g          388 t
ORIGIN      
        1 cgaacgccta cggccaggtg gacatggaac tcctcgcccg tgccgccggg atccgacgga
       61 cctgcaaggt gcaagatgag caggaactga gagatgcctg ggaagaccgg gggcgggggc
      121 cgaccttcat ccacgttgtg ttgaagcccg ggaacgcaac ggtcccgaac atcccgctcg
      181 ccccggccgg gatccgggag cgatttgggc gggcgctccg ggggtaagag actccctgac
      241 ccacgcctct cagccctcct cgcggagtgc ctctatcagg cgtttcctca gggtctcgac
      301 agtcctctcg ctcatcccat tgacgatccc cgtacacttt accagaaacc gcggagcggc
      361 ccggatcagt tcttcacaga aggggtccgg gatctcgcgg gagacgtagt actgtgcatc
      421 gaccctcgct ccccgcgctc tctctacgag cgccatctct gcaggggcga aataatcgga
      481 taaaagccgc tccataagca ggatggtgca ggaatggttc tcgcaccgta ctccgagtcg
      541 tgtgaggata gcgtagaggg agaagtacat cgcatagtat gcagtcgtta tcttccagtc
      601 gcgggccgtg attgtctgca tcgtctcaag ggcttcctcg gccttcttca ggtatgccga
      661 tgccagggtt tcgtttgggg cagtaaggga gatcccgttc ttgatccggc cgcaccacac
      721 gagttgatcc actatcccac cgcctgttcg acgaagtgtt cggtgccttt gatcagcaca
      781 tgattccgga gcacttcccg gagaaaggga tccgtgtgga ggctgcggtc aaagatctcc
      841 agagggtaga cggtgaggtg aatcgggaga ccgtaagtct ttccgaccct ctcgatctca
      901 tcccttcggc attctccggc gatgaagaga tcaaggtcgg agtcctcgtc cgcggtcccc
      961 cctgcgtggc tcccgaagac cgcaccgcat ccctcgataa gggggatgat ccggccgagg
     1021 accccggcga tgaagggttc ctcctcccca aaacagatct tccggtaggt ttctgcaagg
     1081 atgcagaagt cccgcgcgtg gttgccccgc tgcagggaga acagccgcgt tctgcccctg
     1141 gtgacggagc ggaggacctg ttttccttca aggtattcaa gggccgtctg ggctgtgccg
     1201 cggctgatgg gaagatacct gcagacctcc cggatataga cctcccgatc gtagccccag
     1261 gtgaagaggg agaggaccct gaggtggtcc tcccgaatgt ccaatttttt agacatatgt
     1321 ccagaatatt ggacggtccc tggcatcagg gttccggtcg ggagcggctg ccgggatagt
     1381 tcctgccttc atcatccggc ccggctcagg tttccagccc attgcggccg ctttcagggt
     1441 gaatttccac cttccaggcc tgatgtgtag atgattacga gagaactgag gggcaccgcg
     1501 tagcggggct ctatagcggg aatgatatgc gcatgaccta aaaatgagtt ttatctcggc
     1561 ctgaacacag cagctttcct gacggagtca aactccaaaa agaaatcgac ctgttctacc
     1621 cgatccagat gcatcatgca gagatgtggt tttttttcat gggttgagca tgaggttctg
     1681 catcataggg ggtttcctac tggaatctcc gggcaatctc cagagatggt ttccatctac
     1741 tctgagccaa ccaccgctcg ctccggccgg gatccgggag cgatttatgc gggcgatccg
     1801 ggggtgatta cccct
//
LOCUS       DAOQVJ010000378         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_84957_length_1812_cov_3.747866, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000378 DAOQVJ010000000
VERSION     DAOQVJ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84957_length_1812_cov_3.747866"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKL26_07575"
     CDS             complement(<1..277)
                     /locus_tag="PKL26_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83592.1"
                     /translation="MRKKRCPSRTDREDDCQGILPFRTKRARAARNASTAAKPRNPGA
                     GVGVGVGGRGVPVSWTVGVAVVAVSTGLVGEEPAGVGVRVAVGTLVIG"
     gene            295..804
                     /locus_tag="PKL26_07580"
     CDS             295..804
                     /locus_tag="PKL26_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS83593.1"
                     /translation="MNVGTTIIKSRHSVRKYKPDPVDETTIRDVLECARHAPTAMNLQ
                     PWLFGVVRDRDLLKKIADLTDHGKFIADAAICIAVFGEKKAKYYLEDCCAATETIILG
                     LQSYGVGSCWVAGEKKEYAEAVRKLLDVPGNYTLVSLVPAGFPAEIAIQKKKDLDDIT
                     FSDKYKEQQ"
     gene            1369..>1812
                     /locus_tag="PKL26_07585"
     CDS             1369..>1812
                     /locus_tag="PKL26_07585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNS83594.1"
                     /translation="MKRTILQVALDITELDRAVQIAREAVEGGADWIEAGTPLIKSEG
                     MEAVRRIRALFPGREVVADMKVADTGALEVEMAAKAGASVVCILADADDSVIAEAVRA
                     SDLYGVRLMADLINVRDPILRARELEQMGVHIINAHVGIDQQMIGK"
BASE COUNT          369 a          530 c          563 g          350 t
ORIGIN      
        1 tgccgatgac gagtgtgccg accgcgaccc gcactcccac gcctgccggc tcttccccca
       61 cgagcccggt cgagaccgct actaccgcaa cgccaacggt ccaggagacc ggaacgcccc
      121 tgcctcccac gcccacccca accccggcac caggatttct gggtttcgcc gcggtgctgg
      181 cgttcctggc agccctggcc cttttggtgc gaaagggaag gatgccctga caatcgtcct
      241 cccggtcagt gcgggaagga cacctttttt ttctcatgca ccgatggaat ctccatgaac
      301 gtcggaacca cgattatcaa gtcacggcac agcgtgcgca agtacaaacc tgacccggtg
      361 gacgagacca cgatccggga tgtccttgaa tgtgccaggc atgccccgac cgcgatgaac
      421 ctgcagccct ggctcttcgg cgtcgtccgc gacagggacc tcctcaaaaa gatcgccgac
      481 ctcaccgacc acgggaagtt catcgccgat gctgcgatct gcattgccgt gtttggtgag
      541 aagaaggcca agtactacct tgaagactgc tgtgcggcga ccgagaccat catcctcggc
      601 ctccagtcat acggcgtcgg cagctgctgg gttgcggggg agaagaaaga gtatgccgag
      661 gcagtcagga agctcctcga tgtgccgggc aactacacgc tcgtctcgct ggttcctgca
      721 gggttcccgg ccgagattgc gatccagaag aagaaggacc tggacgatat cacgttctcc
      781 gataagtaca aggagcagca ataggcacaa tgcctattgc tgcggggggt gtgcaggcgg
      841 gtgttccagg aatacccttt tcatcgggca aacccgaccg cacttcaccc ccggtgatgc
      901 gccaggagct ttccgcacac gcgtgcgcgt cactgaaatg cgatttgggc ctatgcgagg
      961 gagcagttgg gggatggagc ccccactgcg gggttgtgcg aggaatggcc ccggatcact
     1021 ctgtttttcc tgggtccttt ctgctccggg atttcatttt tccttctcgc actgcggcga
     1081 tcgctatgtc gccttggttt cgaaaggcga agcccgagta aagatgatga aaaatgcagg
     1141 gatttgttca gtcagtgttt tttttggaac accattggac tgcggcgacc ttcaggtcgc
     1201 ccgggggtcg aggggcgcca gcctgagtaa agatgatgaa aacgcaggga tatgttcagt
     1261 cagttttttt taggaacacc attcggtgac cgccaggtcg cctgggggtc gaggggcgcc
     1321 agcccccggt cagcaacaat ttagcctagg ccacaccaca tctctcacat gaaacggacg
     1381 atcctccagg tagccctgga catcacagag ctcgaccggg ccgtccagat tgcccgggag
     1441 gcggtagagg gaggggccga ctggatcgag gcagggactc cgctcatcaa gagcgaaggg
     1501 atggaggcgg tgaggaggat ccgcgccctc tttcccggcc gcgaggtcgt ggctgacatg
     1561 aaggttgccg acacgggggc gctcgaagtg gagatggcgg caaaagcggg agcctctgta
     1621 gtctgcatcc ttgccgatgc ggacgactcg gtgatcgcgg aggcagtccg tgcttccgac
     1681 ctgtatgggg tccggctgat ggccgacctg atcaatgtcc gggacccgat cttgcgtgcg
     1741 agggaacttg aacagatggg cgtgcacatc atcaacgccc atgtaggcat cgaccagcag
     1801 atgataggga ag
//
LOCUS       DAOQVJ010000379         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_85219_length_1809_cov_3.798176, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000379 DAOQVJ010000000
VERSION     DAOQVJ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85219_length_1809_cov_3.798176"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PKL26_07590"
     CDS             <1..1111
                     /locus_tag="PKL26_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNS83595.1"
                     /translation="ETIAGLGEVLVHGVAIKPGKPVIIGKIQSKPVIGMPGYPLSALT
                     VLREIVAPLVRLSGIPVPEPAVIAARLTATLHSEAGSDEFVLLAAGRIGSSWVATPLS
                     RGSGVQMSAVRSNAYLQVISSLEGYEAGEMVPVSLTVSPPEAGEAILVTGSHDPVLDH
                     LAELVKRDHVEVHSNHVGSMGGIIALKRNDCHAAPIHLLAPDGDYNIPYLMKYLPREE
                     VELICVAGREQGIVSQNGISIDDLPGTSFINRQKGSGTRVLLDHELRLREISPGDIPG
                     YEREATTHIGVALAVKTGEAEAGMCVYSAAKALGLPFVPVAKERYELAIRREHMEDPR
                     IRSLLDAIRSPHFREVLGRLGGYDDSITGVQRKVP"
     gene            complement(1083..1538)
                     /locus_tag="PKL26_07595"
     CDS             complement(1083..1538)
                     /locus_tag="PKL26_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNS83596.1"
                     /translation="MQEPAAQVAVIGPSECTEDEAAAAAVIGGVISAHGATLISGGLG
                     GVMEASCRAAKENCGLVVGILPGYGEGNPSLSVSIRTGLSHTRNFLVVESADAIVAVG
                     GAYGTLSEIAIALKRGKPVFGYRSWDIEGVQPCKTPEEAALRALSAARR"
     gene            complement(1541..>1809)
                     /locus_tag="PKL26_07600"
     CDS             complement(1541..>1809)
                     /locus_tag="PKL26_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS83597.1"
                     /translation="KEAIDSANGVAFGLQMGIFTQNIQRIIKTFSLAEYGGVQVNDIP
                     TFRVDHMPYGGVKGSGLGREGPRYAIEEMTVPRLLAFNPAGGVE"
BASE COUNT          335 a          589 c          539 g          346 t
ORIGIN      
        1 ggagacaatc gcaggtcttg gcgaggtgct tgtccacggc gtggcgatca agccgggaaa
       61 gccggtcatc atcggtaaga tccagtccaa gcccgtgatc ggcatgccgg gttacccgct
      121 ctctgccctg actgtgcttc gggagatcgt cgctcccctt gtccgcctct ccggtatccc
      181 cgtcccggag ccggccgtca tcgccgcgag gctcactgcg actcttcact ctgaggcagg
      241 aagtgacgag tttgtccttc ttgcagccgg acgcatcggc tcctcctggg tggcaactcc
      301 cctctcccgg ggatcgggtg tccagatgag cgcggtccgg tcgaacgcct acctccaggt
      361 catctcgtcc cttgaggggt acgaagcagg cgagatggtc ccggtcagcc tgacagtcag
      421 tcccccagag gcaggggagg cgatcctcgt caccgggagc catgatccgg tcctcgatca
      481 cctcgcggaa ctcgtaaaga gggaccatgt cgaggtccac tccaaccacg tgggcagcat
      541 gggagggatc attgccctga agaggaatga ctgccacgcc gcgcccatcc acctgctggc
      601 gccggatggc gactataata tcccctactt gatgaagtac ctcccccgcg aggaggtgga
      661 actcatctgc gtagcgggga gagaacaggg gattgtgtcc caaaacggta tctctattga
      721 cgatctcccc gggacttcgt tcatcaacag gcagaaagga tcaggtaccc gcgtgctcct
      781 cgaccacgaa ttgcggcttc gggagatatc acccggagac atccctggct atgaacggga
      841 ggcgaccacc catatcgggg tggccctcgc agtcaagact ggtgaggcag aggcagggat
      901 gtgcgtctac tcggcagcaa aggcactcgg cctccccttt gttccggtgg caaaagaacg
      961 gtacgagctt gcaatccgcc gggagcacat ggaggacccc cgcatcaggt cgcttctgga
     1021 tgcaatcagg tcgccacatt tcagggaagt ccttgggcgg ctgggcggct acgacgattc
     1081 catcaccggc gtgcagcgga aagtgccctg agcgccgcct cttccggggt tttgcagggt
     1141 tgcaccccct cgatgtccca ggatcgatag ccaaagaccg gctttcccct cttcagcgca
     1201 atggcgatct ccgagagggt accgtaggcc cctcccaccg ccacaatggc atctgcactc
     1261 tcgacaacga ggaagttcct cgtgtgggag agaccggtgc ggatcgagac cgagagggag
     1321 gggttcccct cgccgtaccc agggaggata cccaccacga ggccgcagtt ttcctttgca
     1381 gccctgcagg aagcctccat cacgccgccg agcccgcctg agatgagggt tgccccgtgg
     1441 gctgagatca cgcccccgat cacggctgca gcggcggcct cgtcttccgt gcattccgag
     1501 ggccctatca ccgcgacctg cgccgccggt tcctgcatcc tcattccacc ccgccggccg
     1561 ggttgaaggc aagcagtctt ggcacggtca tctcctcgat ggcatacctc ggcccctccc
     1621 gccccaggcc cgatcccttc acgcccccgt atggcatgtg gtcgaccctg aacgtgggga
     1681 tgtcgtttac ctggacgccc ccgtattctg caagggagaa agttttgatg atccgctgga
     1741 tgttctgggt aaatattccc atctggagac cgaacgcgac tccgttcgcg gaatcaatcg
     1801 cctctttca
//
LOCUS       DAOQVJ010000380         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_85697_length_1803_cov_3.204805, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000380 DAOQVJ010000000
VERSION     DAOQVJ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85697_length_1803_cov_3.204805"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKL26_07605"
     CDS             <1..302
                     /locus_tag="PKL26_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83598.1"
                     /translation="ALAFGGIRPTLTDALNVTGCEIGNAGASGRLGRTSAERALEEYI
                     SLVSRAVAASGARMVVGTGYLAQYLVPRIARHAGASFTIPPHADCANAVGVAVSA"
     gene            299..586
                     /locus_tag="PKL26_07610"
     CDS             299..586
                     /locus_tag="PKL26_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83599.1"
                     /translation="MTLTLHARFDSGRGAVVFNGEPQELRTLGDDESVRDRCRAEVKQ
                     RAIAAGADPRDVEDVRVLHFHAYDVVRSSFRSARIADVVVQIAPGITAEAP"
     gene            583..1662
                     /locus_tag="PKL26_07615"
     CDS             583..1662
                     /locus_tag="PKL26_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNS83600.1"
                     /translation="MKTALVFHPKYLLHEQSRDHPERKERLSYTLDQLREEGLFDHPS
                     VEVFTPSEAPMEAVGRVHIPEYLRFLEQASRRGGFIDFDTNVPPGLFSHALLAAGGAL
                     RAADAVLSGEARNAFALVRPPGHHARPGTGAGFCYLNNVAIMVRHVQARGVKKVLILD
                     WDAHHGDGTQQIFYEDPTVLFTSVHQWPFYPGSGAVEETGSGKGAGFTANMPVPAGTG
                     DESYHFLFDTVIATLAEEFSPDLIAISAGQDNHFTDPLAGLALTARGYADLMKKAVDL
                     AEDLCGGRLVAVLEGGYSVEGGLPYTNLGIIAAMAGLDLRNIREPEGYGEWLTRAGDP
                     SAHAVVKEHAAALRANLAPFWQCFR"
BASE COUNT          347 a          527 c          583 g          346 t
ORIGIN      
        1 acgcccttgc atttgggggg attcggccca ctctgacgga tgcgctgaac gtgacgggat
       61 gcgagatagg aaatgccggg gcttcgggac gactcggtcg gacgtccgcc gaacgtgcac
      121 tggaggagta tatctccctc gtgtccaggg ctgttgccgc aagtggcgca cggatggttg
      181 ttggcacagg ttaccttgca caatacctcg ttccccgaat cgcgcggcat gccggcgcgt
      241 ccttcaccat accgccgcat gcagattgtg cgaatgccgt aggggtcgcg gtctccgcgt
      301 gaccctcacc ctccatgcga ggttcgactc tggcaggggt gccgtggtct tcaacggtga
      361 accccaggag ttgagaacgc ttggggacga cgagtcagtg cgtgaccggt gccgggcgga
      421 ggtgaaacag cgggcgattg ccgcaggcgc cgatccccgt gatgtcgagg atgtgcgggt
      481 gctccatttc cacgcgtacg acgtggtgag aagctcgttc cgcagtgcgc ggatcgcaga
      541 cgtggtggtc cagatcgcgc cgggtattac ggcggaggcc ccatgaaaac cgcgctcgtc
      601 tttcacccaa aatacctcct gcacgagcag tcccgggacc acccagagcg aaaggagagg
      661 ctttcgtaca cactcgacca gcttcgcgag gaggggctgt tcgatcatcc gtctgtggag
      721 gtgttcacgc catccgaagc tcccatggag gcagtcggcc gcgtgcatat ccccgagtac
      781 ttgcggttcc tggagcaggc gagcagaaga ggcgggttca tcgattttga tacgaatgtc
      841 cctccaggcc tcttctctca tgccctgctt gcagccgggg gtgcgctccg ggctgccgat
      901 gcggtccttt caggggaggc gaggaacgcg tttgccctcg tgaggccccc tggacaccac
      961 gcgcgcccgg ggaccggtgc agggttctgc tacctgaaca acgtggccat catggtgcgg
     1021 catgtccagg cacggggagt caaaaaggtg ctgatcctgg actgggatgc acatcacggc
     1081 gatggcaccc agcagatctt ctacgaagat ccgaccgtgc tcttcacttc agtccaccag
     1141 tggccattct atcctggatc gggagccgtg gaagagacag ggtcagggaa gggcgcggga
     1201 ttcaccgcga acatgccggt ccccgcaggg acaggcgacg agagttacca ttttctcttt
     1261 gacaccgtga tcgcgaccct ggcagaggag ttctcaccgg acctgatcgc catctctgcg
     1321 ggacaggaca accatttcac cgaccccctt gccggccttg ccctcaccgc gaggggatat
     1381 gcagacctga tgaaaaaagc tgttgacctc gccgaagatc tctgtggagg gagactggtc
     1441 gcagtcctcg agggcgggta cagcgtcgaa ggaggcctcc catacacaaa ccttgggatc
     1501 attgccgcga tggcgggcct cgaccttcgg aatatccgcg agccggaagg ttacggggaa
     1561 tggctgacaa gggcagggga tccctcggca catgcagtgg taaaggagca tgctgccgcg
     1621 ctcagggcga atcttgcacc tttctggcaa tgtttccgat gatatctgcg gcaacggctt
     1681 gtcctggacg tatggtgggg atgaaatagg aaaaacgcct acatgaactc gcctatggta
     1741 tcatcgcgca tacggatcgg gctggtccag gctgccgccg gacctgaccc tgcagggaac
     1801 ctt
//
LOCUS       DAOQVJ010000381         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_85900_length_1800_cov_4.438395, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000381 DAOQVJ010000000
VERSION     DAOQVJ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85900_length_1800_cov_4.438395"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PKL26_07620"
     CDS             <1..1499
                     /locus_tag="PKL26_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS83601.1"
                     /translation="SSRDIPLVRLKNERKQRGSSQFTLQAGDTIVVEDSHQRMEFPLQ
                     GSSSLPALFSDLGRNLTQLMKGRNARRDFEASFGHPYDVISSAPVPLSILTHPDISWM
                     NEEMAHILGYSPSTMAGRPFHSILPREEDMRWFGELGRQSKGGAGWVQREVVLRKRDG
                     AHVPIRLKMRKLAGKEGEDMYLCICEDIRCHQSLLELQRSFDQKVMEIESRYRAVFES
                     MGAIVIRTDSDGNIVYCNPAAEMMFGFRSVDVAGSNVIGTLVPPGSQSARDFMTMLSG
                     CSRNADESSVIAVEHALPGLRRACIAWKMVGVERDGVVPDIVCVGEDITDYSRPDSCG
                     IRTDPWRTRLLKGTDIKRDVFDDVFSICVEISREGWEGKRVGTSFLVGDASSVMAKSQ
                     PLILNPFNSMENEMRYVGNPGNKTCIKNFAMLDGGFIIGGDGFIVASGRQFLPGDVPL
                     ALPAGYGTRHRSVAAMTVVTGAVGIVVSETGGRLTVFREGRSVKSLIF"
BASE COUNT          379 a          497 c          528 g          396 t
ORIGIN      
        1 cgtcatcccg cgacatcccc ctcgtaaggc tgaagaacga gcggaaacag cgtggttcgt
       61 cccagttcac cctccaggca ggggatacta tcgtggtgga ggactcgcac cagaggatgg
      121 agttcccgct ccaggggtct tccagcctcc cggcactctt ctctgacctg ggcaggaacc
      181 tcactcaatt gatgaaaggc aggaatgcca ggcgcgattt tgaagcgtcg ttcgggcatc
      241 cttacgacgt catctcttcg gctcctgtcc ctctctctat cctgacacat cccgatatct
      301 cctggatgaa cgaagagatg gcgcatatcc tgggatactc tccctccacg atggcgggga
      361 gaccgttcca ttccatcctc ccccgcgaag aggacatgag gtggtttggg gagctgggaa
      421 ggcagtcgaa gggcggtgcg ggctgggttc agagagaggt agtgctcagg aagagggatg
      481 gtgctcatgt ccccatcagg ctgaaaatgc gaaagcttgc cgggaaggag ggggaggaca
      541 tgtatctctg catctgcgag gacatccgct gccaccagtc gcttctcgaa ctgcagcggt
      601 cttttgacca gaaggtcatg gagatcgaga gcaggtatcg cgcggttttc gagagcatgg
      661 gggcaattgt catccggacg gactctgatg ggaatattgt gtactgcaat cccgcagccg
      721 agatgatgtt tgggttccgg tcggttgatg ttgcaggaag caatgtcatc ggcacgctgg
      781 ttccacccgg ttcgcaatcg gcgcgcgact tcatgacaat gctttccgga tgttcgagga
      841 acgcagatga atcttcggtc atcgcggtgg aacatgcgct ccccggcttg cgcagggcct
      901 gcatcgcgtg gaagatggtg ggggttgaga gagacggggt cgtcccggac atcgtctgcg
      961 taggcgaaga catcaccgat tattcccgcc ctgactcgtg cggcatccgc accgacccct
     1021 ggaggacacg cctcctcaaa gggaccgata tcaagcggga tgtctttgac gatgtcttct
     1081 ccatatgcgt cgagatctcg cgggagggct gggagggaaa acgggtcggg acatcgttcc
     1141 tggttggcga cgcctcctcg gtcatggcca aatcccagcc gcttatcctc aacccgttca
     1201 attcaatgga gaacgaaatg aggtatgtcg gaaaccccgg gaacaagacc tgcatcaaga
     1261 attttgcgat gctcgacggc gggttcatca tcgggggaga cgggttcatc gtggcgtccg
     1321 ggcggcagtt ccttccgggt gacgtccccc tcgcgcttcc tgccgggtac ggcacccgcc
     1381 accgctctgt tgcggcgatg accgtagtga ccggcgcggt gggaattgtt gtctctgaga
     1441 cagggggccg cctgacggta tttcgcgaag gccggagtgt aaaatcgctg attttttaaa
     1501 actgcacagt tccctcccac tatacatgca caacaagaaa agattgcatg cgtgacattc
     1561 atatgtaaaa cataaaaccc caattgattg tgaaggatcc ttaaatcccc gtatacgaag
     1621 cgcttttttt aatcctggga gtcattcgta ccggtaaaag aagcatgaac cccactcgat
     1681 cttctcacct gggctcatgt gtttgagccg gtcctctgca tgcacttcac agactaccga
     1741 cccgcagcat cccagtacct ggatgccgat ggctttcctc ccgcatacat cgcaggtctt
//
LOCUS       DAOQVJ010000382         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_86220_length_1796_cov_3.049397, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000382 DAOQVJ010000000
VERSION     DAOQVJ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86220_length_1796_cov_3.049397"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PKL26_07625"
     CDS             complement(<1..1249)
                     /locus_tag="PKL26_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNS83602.1"
                     /translation="MKTISAFIVASVLICSFAAGVHGETTTEGSDGSLITLQGNTITL
                     EGSGAKIGASTVTITSGGTYTICGTLNNGQVIVDCPDTEKVTLVLSGAHISCATSAPI
                     YVASADKAVITLADGTSNSVTDGSSYVFPDAASDEPNAAIFSKDDLTINGGGALSVTG
                     NYNDGITSKDDLKITGGSVTVNAKNDGVRGRDSVVVREGTLTITASGDGIQSNNDEDA
                     EEGYVSIESGTISIITGADGIQAESTLAISGGSITITSGGGAGNGNTATPRDNQGRST
                     ITVESDTGSAKGLKAGAALEVSGGSITINSADDAIHSNGSITIDGGTMTLASGDDAIH
                     ADSSIETNGGYIDITNCYEGLESEIITLNEGTIHLTSRDDGINAVSKTAVAVTARQPG
                     QQPFEASGNCALGIHGGYLVVNAG"
     gene            1546..>1796
                     /locus_tag="PKL26_07630"
     CDS             1546..>1796
                     /locus_tag="PKL26_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS83603.1"
                     /translation="MTSRDDPGKHHQHSVFSASHAAHLDTRFRRILYRPDHLSERYVK
                     QGDRVLDFGCGPGFFTREFAKRAGDTGRVYAVDLQDEML"
BASE COUNT          350 a          530 c          485 g          431 t
ORIGIN      
        1 ctcctgcatt aaccacgaga tatccgccat gaatgcccag ggcacagttc ccggaggcct
       61 cgaacggctg ctggccaggc tgcctcgccg tcacggcaac agcagtcttc gatacggcgt
      121 tgatcccgtc atcgcgggat gtgaggtgaa tcgtcccctc attaagtgtg atgatctcgc
      181 tctccagccc ctcgtagcag ttcgtgatat cgatataccc gccattggtc tctatcgagg
      241 agtccgcatg gatagcatcg tcaccggagg caagggtcat tgtgccacca tcgatcgtga
      301 tgctgccgtt ggagtggatc gcgtcgtcgg ccgagttgat ggtgatcgaa cctccgctca
      361 cttccaacgc tgcccctgct ttcagccctt tcgcgctgcc cgtatcgctc tcgacagtaa
      421 tggtgctcct gccctggttg tcccggggag ttgcggtgtt accgtttcct gcacctcctc
      481 cggatgttat cgtgatactg cccccgctga tcgcgagggt agactctgcc tggataccgt
      541 ccgccccagt aatgatagag atggtgccac tctcgataga gacgtagccc tcctcggcgt
      601 cctcgtcgtt attggactgt atcccgtcac cgctcgcggt gattgtaagg gtcccttccc
      661 tgacaaccac cgagtccctg ccgcggacac cgtcgttctt ggcattgacc gtgacgctcc
      721 cgccggttat cttcaggtcg tctttgctgg taatgccgtc attgtagttc ccggtgaccg
      781 agagggctcc gccaccgttg atggtgaggt cgtctttcga gaagatggca gcgttcggtt
      841 catccgaagc ggcatccggg aagacataag aacttccgtc cgtcaccgaa ttggaagttc
      901 cgtccgcaag ggtaatcaca gccttgtcgg cactggccac gtaaataggg gcgcttgttg
      961 cgcaggagat atgagctccg gaaaggacaa gggtgacttt ctccgtatcg gggcaatcga
     1021 caatgacctg tccattgttc agagtgccgc atatcgtgta ggttccgccg gaagtgatgg
     1081 tgaccgtcga ggctccaatt ttcgcaccgc ttccttcgag tgtgattgtg ttgccctgca
     1141 atgtgatcag ggatccatcg gagccttctg tcgtcgtctc gccatgcacg cctgcggcaa
     1201 acgagcagat caggacggat gcaacgatga atgcagatat ggtcttcata gaggaatctc
     1261 tcctgttttt ctgggtattt tcttgaattt actctgcagg cctcctatcc cgcagaggat
     1321 atttccttcg cgtggggaag acccgctcat ttgacaatgt aaagtataca atacattgtg
     1381 taaatatctt ttgccaagaa tcttcaatcc agtaccccgc atagcagcgg gaaagaggca
     1441 ggccgccctc tccggcagaa gcgttcatca cccctcgttg ggaggaaaac cgatcccaca
     1501 attattacag aactgttatc gggacgctgc gcacatactc ctcgcatgac ttcccgcgat
     1561 gaccccggga aacaccacca gcacagtgta ttctccgcat cccatgctgc acaccttgat
     1621 acccggttcc gtcgcattct ctaccgccct gaccatctct cggaacgata cgtcaagcag
     1681 ggtgaccggg tgctcgattt cgggtgcggc ccggggttct tcacccgcga atttgcgaaa
     1741 cgggcaggcg atacaggccg ggtctacgct gtcgacctgc aggacgagat gctcag
//
LOCUS       DAOQVJ010000383         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_86593_length_1791_cov_4.368664, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000383 DAOQVJ010000000
VERSION     DAOQVJ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86593_length_1791_cov_4.368664"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PKL26_07635"
     CDS             <1..864
                     /locus_tag="PKL26_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNS83604.1"
                     /translation="AVWYYLLLASLVCLAAWWVRGLRYRSILASLGIPVGVNFSTACI
                     FVSQTANLIVPARLGDLVRIFILGHEKNAPVSKGISSLVVERVFDIVTVALLGAVSLL
                     FVLNVPDWFYTLILLPLAAGAVFFAVLLFIGKFNSENRIIKTLLTIMEQIKEASLSVR
                     AVVILSGSSILIWLMDVMVCVFVVMMFQEEIPFAAVVLAIVIGNLVKAVPLTPGGVGT
                     YELALALTFTLVGVPAAEAAMIAVIDHLVKNLITLVGGIASIYYFGGWVLDSIKSAFE
                     KRFGGIKPGGV"
     gene            complement(961..1104)
                     /locus_tag="PKL26_07640"
     CDS             complement(961..1104)
                     /locus_tag="PKL26_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83605.1"
                     /translation="MEYSPFHAARRKRIAKGARARSIGRRRSPQYQESREKTRRDAGN
                     ETG"
     gene            1037..>1791
                     /locus_tag="PKL26_07645"
     CDS             1037..>1791
                     /locus_tag="PKL26_07645"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNS83606.1"
                     /translation="MLLALAPFAILFLRAAWKGEYSKKAFEGQGVWVFLFLLFFFAMV
                     SVRFVNPSISYAEKFMDHAFLASIMRVPVVPPPDPWFLGGYLNVYYYFGYWMIGAVGI
                     VSGVPSYITFNLALPTVLGLSAMMMYALGRLLTRRFAYLTLATFLVANPSFIWQIIQG
                     KSLGTVFWDSTRTIPDTINEYPLFSFLWGDVHPHVIGIFNQVLFLFILVYAWTRWKSL
                     QPQDRMGVILLAALSLGAMPLINTWDVIVYAPVT"
BASE COUNT          293 a          565 c          474 g          459 t
ORIGIN      
        1 gccgtgtggt attacctcct ccttgcctcg ctcgtctgcc tggccgcctg gtgggtgagg
       61 ggtcttcgat accggagcat cctcgcttcg ctcgggatcc ctgtcggagt gaacttctcc
      121 acggcatgca tcttcgtatc gcagaccgca aacctgatcg tgcccgcaag gctcggcgac
      181 cttgtaagga tcttcatcct cggacatgag aagaacgccc cggtttcaaa ggggatctcg
      241 tccctcgtcg tggagcgggt attcgacatc gtcacggtcg cccttctcgg ggccgtatct
      301 ctcctcttcg tcctgaatgt ccccgactgg ttctataccc tcatcctgct cccgctcgct
      361 gccggggcgg tcttcttcgc agtactgctc tttatcggca agttcaactc tgagaacagg
      421 atcatcaaga ccctgctcac catcatggag cagataaaag aagcctctct atccgtccgg
      481 gcagtagtca tcctctcagg cagttccatt ctcatatggc tgatggacgt gatggtctgt
      541 gtcttcgtgg tgatgatgtt ccaggaggag atcccattcg cggcggtggt cctcgccatc
      601 gtcattggaa acctcgtgaa ggcagttccc ctcactcctg gcggggtagg gacgtatgaa
      661 ctggcccttg cgcttacgtt cacccttgtg ggagtcccgg ctgcagaggc cgcgatgatc
      721 gcagtgatcg atcacctggt caagaacctt atcacgcttg tcgggggtat cgcctctatc
      781 tattattttg gcggatgggt ccttgacagc ataaagagcg ccttcgagaa gcgattcgga
      841 gggataaaac ctggcggggt atgagttcca ggccctggca gtcatctcct ggctgctcct
      901 gctcttcttc ctccagctgg ctctctggcc gtacctgaag ggggcattcc gcgagttcgc
      961 ctacccggtc tcgttcccgg catccctcct tgtcttctcc ctgctctcct ggtattgcgg
     1021 gctcctccgc ctcccgatac tcctcgccct cgcccccttc gcgattctct ttctgcgggc
     1081 cgcgtggaag ggtgaatatt ccaagaaagc cttcgaaggc cagggggtat gggtgtttct
     1141 cttcctgctc ttcttctttg cgatggtgag tgtcaggttc gtcaacccga gcatctctta
     1201 tgccgagaag ttcatggacc acgcgttcct cgcctccatc atgcgggtgc cggtcgttcc
     1261 accgccggac ccctggttcc tcgggggata cctgaacgtc tattattact tcggatactg
     1321 gatgatcggt gcggtcggga tcgtgagcgg ggtgccttcg tatatcacat tcaacctggc
     1381 actcccaacc gtgctcggtc tctcggctat gatgatgtat gcgctgggac gtctcctgac
     1441 gcgccggttc gcgtacctta cccttgccac attcctggtt gcaaacccat cttttatctg
     1501 gcaaatcata cagggaaaga gccttgggac cgtcttctgg gacagcacac ggaccatccc
     1561 cgacaccatc aacgagtatc ccctcttctc attcctgtgg ggggacgtcc atccccacgt
     1621 gatcgggatc ttcaaccagg tgctcttcct cttcatcctc gtctatgcct ggacccgctg
     1681 gaaatcgctc cagccgcagg accgaatggg agtcattctc cttgcggcgc tcagcctcgg
     1741 cgccatgccg ctcatcaaca cctgggacgt catcgtctat gcccctgtga c
//
LOCUS       DAOQVJ010000384         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_86667_length_1790_cov_4.259942, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000384 DAOQVJ010000000
VERSION     DAOQVJ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86667_length_1790_cov_4.259942"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PKL26_07650"
     CDS             complement(<1..1257)
                     /locus_tag="PKL26_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNS83607.1"
                     /translation="MKKTIHQINERIRDGNARVVTAEEMPAIVAELGEEGALAEVDVV
                     TTGTFGAMCSSGGFFNFGHADPPIRMERVWLNDVEAYAGLAAVDAYIGATQQSTTRED
                     QYGGAHVIEDFIAGKSVELRAISRGTDCYPRRTITTDLLLENLNQAIMCNPRNGYQRY
                     NAASNSTERVLHTYMGMLLPSFGNVTYSGAGLLNPISNDPGFRFIGSGVPCFLGGAEG
                     MVIGEGTQASPAAGFGTLMVTGDMKKMNTEYIRAATMHGYGVTMYVGLGIPIPVLDVE
                     TVRSTAVKDEDIWVDIIDYGVPARDRPTIGKVNYAMLKAGHLEINGEEVRTSSLSSLR
                     RARRVAEELKGWIESGKMAVCLPTRRIDPSKRAVPMRETVQGPRVLEIMDRKVITISE
                     DEPIRDAAQKLLKGETNHLPVINDNGV"
     gene            1348..>1790
                     /locus_tag="PKL26_07655"
     CDS             1348..>1790
                     /locus_tag="PKL26_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNS83608.1"
                     /translation="MDIGEFTRIMEALAPPDLAEEWDAEKIGLVVEGVENLDHICCAL
                     DATIATVSKAVSEGADMLVVHHTPLWTPVTAFRGSIAALLRRAVASGMNIYVLHTNYD
                     HAPGGVNDILAGLLDLKDARPLSLGVVGECRLGLQEIADRLCCPLR"
BASE COUNT          337 a          551 c          510 g          392 t
ORIGIN      
        1 caccccgttg tcattgatga ccgggaggtg gttggtctcg cctttcagga gtttttgtgc
       61 ggcgtccctg atgggttcat cctcagatat ggtgatcacc ttccggtcca tgatctcaag
      121 caccctcggc ccctggacag tctcccgcat cgggacggct cttttgctcg gatcgatccg
      181 ccgggtcggg aggcagactg ccatctttcc ggactcgatc caccctttca gctcttctgc
      241 aacccttcgc gccctgcgga ggctcgagag cgaggatgtc cgcacctcct cgccgttgat
      301 ctcaaggtgc ccggctttca gcatggcgta gttcaccttc ccgatagtcg ggcggtccct
      361 cgcagggacc ccataatcaa tgatatccac ccatatgtcc tcgtccttaa ccgcagtgct
      421 cctcaccgtc tcgacatcga gcacgggaat aggaatgccg agccccacgt acatggtcac
      481 tccgtatcca tgcatcgttg cggcccggat gtactccgtg ttcatctttt tcatgtcgcc
      541 ggtgaccatc agggtcccaa aacccgcggc gggagaggcc tgggtccctt cgccgataac
      601 catcccttcg gccccgccga ggaaacaggg gactcccgac cctatgaacc ggaacccggg
      661 atcgtttgag atggggttga ggagacctgc gcccgagtag gtcacgttgc caaacgaggg
      721 gaggagcatc cccatgtagg tgtgcaggac cctctcggtc gagttgctgg ctgcattgta
      781 ccgctggtag ccgttccggg gattgcacat gatcgcctgg ttgaggttct cgaggaggag
      841 atcggtggtg atggtcctcc ggggatagca gtccgtgccg cgggagatcg cccgcagctc
      901 cacggatttt cctgcgatga aatcctcgat gacgtgggcg ccaccgtact ggtcttctct
      961 tgtggtggac tgctgggtcg ccccgatata cgcgtcgact gctgcgagcc ccgcgtacgc
     1021 ctccacatcg tttaaccaga cccgctccat ccgtatcggg ggatccgcgt ggccgaagtt
     1081 gaagaaccct ccggaggagc acatggcccc gaacgtgccg gtggtcacca cgtccacctc
     1141 tgcaagggcg ccttcctcgc cgagttcggc aacaatggca ggcatctcct cggcggtgac
     1201 cacccgggca ttcccgtcac gaatccgttc atttatctga tggatggtct tcttcataca
     1261 ataatattcg ataaagaata tttaaagatg tttcgtatta ctcacagtaa tatactctta
     1321 ataagcctgg acacagatat acctgtcatg gatatcgggg agttcacccg catcatggaa
     1381 gcactggcac cccctgatct tgcggaggaa tgggatgccg aaaagatcgg gcttgtcgtg
     1441 gagggtgtgg aaaacctgga tcacatctgc tgtgcgctgg acgcaacgat tgcaaccgtc
     1501 agcaaggccg tctcagaggg cgctgacatg cttgtcgtgc atcacacgcc cctctggacc
     1561 ccggtcacgg cgttccgggg ctcaatcgcc gcactcctgc gaagagccgt tgcctcggga
     1621 atgaacatct acgtactgca cacgaattac gaccatgccc caggcggggt caacgacatc
     1681 ctggcaggtc tccttgatct aaaagatgca cggcccctct cactcggagt ggtgggagag
     1741 tgcaggctgg gcctccagga gatcgccgac cggctctgct gcccgctgcg
//
LOCUS       DAOQVJ010000385         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_87476_length_1781_cov_4.539397, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000385 DAOQVJ010000000
VERSION     DAOQVJ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87476_length_1781_cov_4.539397"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKL26_07660"
     CDS             <1..911
                     /locus_tag="PKL26_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315178.1"
                     /note="protects formylmethionyl-tRNA from spontaneous
                     hydrolysis and promotes its binding to the 30S ribosomal
                     subunits during initiation of protein synthesis; also
                     involved in the hydrolysis of GTP during the formation of
                     the 70S ribosomal complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNS83609.1"
                     /translation="QGIDILGREGQAVTAAAGIKVSAPNLEGAIAGSPVQVVRGNQEE
                     VTERIRREMEEVNVRLSPEGVIIKADTIGALEALCKELDEKDIGVLRAEVGSVSRHDL
                     IDAETIKNPYFRVLIAFSTSLLPDATEMVKEPGYPVKVFEGRVIYKLVDDYIQWRDEL
                     KRKTEQQRFEQIIHPAKIRVLPHCIFRQSNPAVVGVRVLGGTLRTDVNLIQPDGRKVG
                     HVKSIQLNQETIREAQTGAEVAISVEGATVGRQLDVEDDLLVDIPERHVKVLEKEMLP
                     HLPVHTQEVLAEFTAMRRRENPFWGK"
     gene            1077..1481
                     /locus_tag="PKL26_07665"
     CDS             1077..1481
                     /locus_tag="PKL26_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HNS83610.1"
                     /translation="MAEFKIVLSDQKTGRAYKVDVSGGAAGALIGKHIGDEVDAGSVG
                     LSGYRMEITGGSDRNGTPARRNLPVAGRKKILLSGGAGFKPVMEGQRRRKTIRGNEIT
                     ADFVQINARVTAYGDKALEEYFAKPAAEEKKE"
     gene            complement(1482..>1781)
                     /locus_tag="PKL26_07670"
     CDS             complement(1482..>1781)
                     /locus_tag="PKL26_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HNS83611.1"
                     /translation="HGDMITPLFDVSGVEIPLGYKPGSSVFERSDPVDDLLARISQQA
                     GIARGQLVAELNRLVQERFGGNLRPEAAAVLMAKRYSVEFRDLLPALREHLVKNE"
BASE COUNT          416 a          455 c          581 g          329 t
ORIGIN      
        1 ggcagggcat cgacatcctc ggtcgcgaag ggcaggcggt cactgccgcc gcgggcatca
       61 aggtgagtgc cccaaacctg gaaggtgcca ttgcaggatc cccggtccag gtggtgagag
      121 gcaaccagga agaggtgaca gagaggatca ggagagagat ggaggaggtg aatgtccggc
      181 tttccccgga gggagtgatc atcaaggccg ataccatcgg cgccctggag gcactctgca
      241 aggagctcga cgagaaggat atcggggtgc tgcgggcgga agtgggttct gtgagccgcc
      301 atgacctcat cgacgccgag acaatcaaaa acccgtattt ccgcgtgctt atcgcgttct
      361 caacctccct cctgcctgat gcgaccgaga tggtgaagga acccgggtat cctgtgaagg
      421 tctttgaggg gagggtaatc tacaaactgg ttgacgatta tatccagtgg agggacgaac
      481 tgaagaggaa gaccgagcag caacgtttcg agcagatcat ccacccggca aagatacggg
      541 tacttcccca ttgcatcttc cgccagagca acccggcggt ggtaggtgtg cgggtgcttg
      601 gagggacgct tcggactgac gtgaacctca tccaacctga cgggaggaag gtgggccacg
      661 tgaagtcgat ccagctgaac caggagacga tccgggaggc gcagaccggc gcggaggttg
      721 caatctctgt tgagggtgcc accgtgggga ggcagctcga cgtggaggac gacctgctcg
      781 tggatatccc ggaacgccac gtaaaagtcc tggagaagga gatgctcccc catctcccgg
      841 ttcataccca ggaggtcctt gccgagttca ccgcgatgag gcgaagggag aaccctttct
      901 ggggaaagta gtctttaata tatctttcat ccaatttcat aggtacttca tggaggcttg
      961 gcaccggcct tcggaataat ccttcccccg attgagtagg tggggaggag agaaccggtg
     1021 ccctgcagca tgccggtgaa ccgaaccgga tattcatatg atggagagat caggaaatgg
     1081 cagagttcaa gatcgtcctg tcggaccaga agacgggcag ggcatacaaa gtcgatgtga
     1141 gcggtggggc ggctggagcc ctgattggca aacatatcgg tgatgaggta gacgcaggtt
     1201 cagtgggcct gtccgggtac cggatggaga tcacgggagg ttccgaccgg aacgggacgc
     1261 ccgcgcgcag gaacctgccc gtggcaggga ggaagaaaat cctcctctcg ggcggagcag
     1321 gcttcaaacc ggtcatggaa gggcagcgcc gccggaagac gatccggggg aacgagatta
     1381 cggcagattt tgtccagata aatgcgcgtg tcaccgccta tggtgacaag gcactggaag
     1441 aatactttgc aaagcctgcc gctgaagaga agaaggaata atcactcgtt ttttaccagg
     1501 tgctccctca gggcagggag caggtccctg aattcaacac tgtagcgctt tgccatcagc
     1561 acggctgctg cttcaggccg aaggtttccc ccgaaccgtt cctggaccag cctgttcaat
     1621 tctgcgacga gctggccccg ggcgatgccc gcctgctggg atatccgcgc aagaaggtca
     1681 tcgacggggt cagaacgttc aaaaacggat gaacctggtt tgtatccaag ggggatctca
     1741 acgcctgata catcgaagag cggggtgatc atgtctccat g
//
LOCUS       DAOQVJ010000386         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_88384_length_1770_cov_3.163265, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000386 DAOQVJ010000000
VERSION     DAOQVJ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88384_length_1770_cov_3.163265"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1770
                     /locus_tag="PKL26_07675"
     CDS             <1..>1770
                     /locus_tag="PKL26_07675"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS83612.1"
                     /translation="GSGFCTLYITPLRSLNRDMLQRLEWWCRELGLSVGVRHGDTPQS
                     ERRKQALHPPDLLITTPETLQALFMGKVLRKHLSDVRYVVVDEIHELAGSKRGAQLAV
                     ALERLKEYAGEFQRIGLSATVGNPEDVGRFLCGARDFSIVSVPVAKHLEISVDFAGDS
                     FEKQAAWVSRAIGRHESTLVFVNTRVTAEALGHHLYHRGDVEVHHGSLSKEVRIEAEE
                     RFRSGAVRALISTSSMELGIDIGRIEHVIQFGSPREVARLVQRVGRAGHQMDAVSRGT
                     VLATGFDDLLESLVIARRAKSAQVEDVSLHLNAADVLANQVAALAVEYREISRQKIRD
                     IVERSYPFAMAGNLVDEVCTQMAEHGLIRIEGDMVRSGARSRRYLSSNLSMIHDERKV
                     VVFDMVSRKTVGTLDESFVVGFLHTGAVFITKGQLWRVLDMEEGKVTVEPAKKAKGEL
                     PSWEGEQIPVPFSVAREVGALRRSRDFSPYHAREEPLGFAHRFLVEMDRNRSRVPDDT
                     LITLENAPEGVVCNVCAGHKANEALARVLSILLSARYGTTVGIEIDAYRIYLRLPSSV
                     KAADVREILLSLDPMHLEGILQLA"
BASE COUNT          338 a          516 c          575 g          341 t
ORIGIN      
        1 cgggagcggg ttctgcacgc tctatatcac ccccctgcgg tcgctgaacc gggacatgtt
       61 gcagcgcctg gaatggtggt gcagggaact tgggctctcg gttggggtca ggcatggtga
      121 cacgccgcaa tcagagcgca ggaaacaggc gctccacccc cccgatctcc tgatcactac
      181 ccctgagacc ctccaagcgc tcttcatggg caaggtgctg cgaaaacatc tttccgatgt
      241 acggtacgtg gtggtcgacg agatccatga gcttgcgggg agcaagcggg gtgcccagct
      301 cgcggtggca ctcgagcggc tgaaggagta tgcaggggag ttccagcgga tagggctctc
      361 ggccacggtg ggaaacccgg aggacgtggg ccggttcctc tgtggggcac gtgatttttc
      421 catcgtctct gtcccggtcg caaagcacct cgagatatcc gtcgattttg ccggggactc
      481 tttcgagaag caggccgcat gggtctcccg ggccatcgga cggcacgagt ccacactggt
      541 attcgtgaat acccgtgtaa ccgccgaggc actcggccat cacctctatc atcgcggcga
      601 cgttgaggtg caccacggat cgctctcgaa ggaggtgcgg attgaggcag aggagcggtt
      661 caggtccggt gcggtgcgtg ccctgatctc cacatcatcg atggagcttg ggatcgatat
      721 cggccggata gagcatgtga tccagtttgg gtctccgcgg gaggtcgcac gactcgtgca
      781 gcgtgtggga cgcgccggtc accagatgga cgcggtctcc cggggaacag tgcttgcgac
      841 cggttttgat gacctgctcg agtccctcgt catcgccaga agggcgaaat cagcccaggt
      901 tgaagacgtg agccttcacc tcaatgccgc ggatgtcctt gcaaaccagg tagcagcgct
      961 ggcggttgag taccgggaga tctcccgcca gaagatcagg gacatcgtgg agaggagcta
     1021 cccgtttgcc atggccggaa acctcgttga cgaggtctgc acccagatgg ccgagcacgg
     1081 cctcattcgc atcgagggtg acatggtaag gtccggggca agatcgcggc ggtacctctc
     1141 gtcgaacctc tcgatgatcc acgacgaacg aaaggttgtc gtctttgaca tggtctcaag
     1201 gaagaccgtc gggacgctcg acgagtcgtt cgtcgtcggg ttcctgcaca ccggggcagt
     1261 cttcatcacc aaggggcagc tctggcgggt acttgacatg gaggagggga aggtcactgt
     1321 cgagccggca aagaaggcaa agggcgaact cccctcgtgg gagggggagc agatccctgt
     1381 cccgttctct gtcgcccgtg aagtgggggc gctcaggagg tcaagggact tttctcccta
     1441 ccatgccaga gaagaaccgc tcgggtttgc acataggttc ctcgtggaga tggacaggaa
     1501 cagatcaagg gtccccgatg acaccctgat caccctcgag aacgcccccg agggggtcgt
     1561 ctgcaatgtc tgtgccgggc acaaggcaaa cgaggccctt gcccgcgtcc tctccatcct
     1621 cctctcggca aggtacggga ccaccgtggg gatcgagatc gatgcctacc ggatctacct
     1681 gcgcctccct tcttcggtga aggcggcaga cgtgcgggag atactcctct ctctcgaccc
     1741 gatgcacctt gagggcatcc tgcagcttgc
//
LOCUS       DAOQVJ010000387         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_89180_length_1760_cov_3.872141, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000387 DAOQVJ010000000
VERSION     DAOQVJ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89180_length_1760_cov_3.872141"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKL26_07680"
     CDS             <1..585
                     /locus_tag="PKL26_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNS83613.1"
                     /translation="KARVSADTALGLLFGISMAVAIFFIAYMQTLGKGFSAMVILFGD
                     VISLYQGEIYLLGVICVVAVVFVVMLYKEITAIIFNKKIAESTGVRVRPVYYAMLFVI
                     ALTVALCIPIIGGLLLYVWLVTPAAIALQFCYRLSVMFVVAPLVAAAVSVSGALAGLE
                     YSLPVAPLTAVLFSLVFIVAVVLSPKRRVSFRKF"
     gene            650..1588
                     /locus_tag="PKL26_07685"
     CDS             650..1588
                     /locus_tag="PKL26_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNS83614.1"
                     /translation="MLFIPGAYALNVVSTTTVLWDPVQYIGGEKVHAIYIADPTICPH
                     MQADIIPNRMQMQRDFIRGADLFIAHNGSVDVSYVMPFVDDFMEANHFGTVKWVTLKN
                     PSMIWNTPDGAKALSQEVAGWLIAADPANREYYESRLAEYLAQIGAADISTEERKRIA
                     GQDIVVMVWQQEAAGKWLGMDTVSIYAPEFYMGGKFTPVKIVDDINANITKYQEVRYI
                     IENMQSGELAKGLEEALHTKGIPAKRVIFTNFPKSIPGVDTLPDVIRYNKALVTPEDG
                     ITGQPTGAPTTTPLSCVVVAGGILGAFLLFLNKRED"
BASE COUNT          392 a          464 c          457 g          447 t
ORIGIN      
        1 aaggcaaggg tctcggccga cactgcactc ggcctccttt ttggcatcag catggcggtg
       61 gcgatcttct tcattgccta catgcagacc cttggaaagg gcttttccgc aatggtgatc
      121 ctctttgggg acgtcatctc tctctaccag ggagagatct atcttttagg cgtcatttgt
      181 gtggtcgcag tggtattcgt ggtcatgctc tacaaggaga ttacggcgat catcttcaac
      241 aagaagattg ccgaatcgac aggagtccgt gtaaggccag tctattacgc aatgcttttc
      301 gtcatcgcgc tgacagtggc actctgcatc ccgattatcg ggggactcct tctctacgta
      361 tggctggtta ccccggcggc aattgcactc cagttctgtt accgcctctc tgtcatgttc
      421 gtggtggccc ccctggttgc agccgcagtg agcgtgtcgg gagcgcttgc aggccttgaa
      481 tattccctgc cggttgcacc tctcactgca gtcctcttct ctctcgtatt cattgtcgcg
      541 gtagtcctct cgccaaagag acgcgtttca tttcgaaaat tttaatgtca tcctcgcaaa
      601 aaatgaaaac atgaataaaa aatttactct attcatcaca tttttgatat tgttgttcat
      661 accaggagcg tatgcactga acgtggtctc cacgaccacg gttctctggg acccggtcca
      721 gtatatcggg ggagagaagg ttcatgccat ctacattgcc gatcctacaa tatgccccca
      781 catgcaggcc gacataatcc cgaaccgcat gcagatgcag agagacttca tcaggggcgc
      841 ggatctcttc attgcccaca atggatccgt cgacgtctcg tatgtcatgc cttttgtgga
      901 tgatttcatg gaggccaacc atttcggtac tgtgaaatgg gtcaccctga aaaatccctc
      961 gatgatatgg aatactcctg atggagcaaa ggccctctcg caggaggttg ccggctggct
     1021 cattgctgca gatcccgcga acagggagta ttatgaatcg cgattggccg agtacctcgc
     1081 gcagatcggt gccgctgata tctcaacgga ggagaggaaa cggattgccg gccaggacat
     1141 cgtggtgatg gtctggcaac aggaagcggc aggaaagtgg ctgggtatgg acacagtgag
     1201 catctatgcg ccggaattct acatgggagg gaagttcact ccggtcaaaa tcgtcgacga
     1261 tatcaatgcg aacatcacga agtaccagga agtccggtat atcatcgaga atatgcagtc
     1321 tggcgagctt gccaagggcc tcgaggaagc actccatacc aagggaatcc ccgcaaagag
     1381 ggtgatcttt acgaatttcc cgaagtcgat ccctggtgtt gacaccctgc ccgacgtcat
     1441 ccggtataac aaggctcttg ttactcccga ggatggaata acgggtcagc caacaggtgc
     1501 cccgaccacc acaccactct cgtgtgtcgt ggttgcaggg ggcatccttg gggcgttcct
     1561 gctattcctg aataagagag aggattgaga tcctctctcg attccggttc actttttaat
     1621 aggaccacac gtttcagaat gcggcattgg aatgcatggt gttggcggat ttccggcagg
     1681 gtccctcgcg ccatctcctt attcttttaa tcccggattt tcacctcatt tccgccgcaa
     1741 gtaaaatgcc gccatttggc
//
LOCUS       DAOQVJ010000388         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_89417_length_1757_cov_5.266745, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000388 DAOQVJ010000000
VERSION     DAOQVJ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89417_length_1757_cov_5.266745"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1181
                     /locus_tag="PKL26_07690"
     CDS             72..1181
                     /locus_tag="PKL26_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNS83615.1"
                     /translation="MATMKIRGGDLDLVEYEFNSFPPGKQIRTLGIERPASLTPVKGT
                     VKVRAPARIHLTVLDMNRFSPARPGGGGVGFAIQLYCSVEARCIERGVEIDYNRPAII
                     RHFVEVFREISGYEGGFAIRATDHQHQHVGLGSTSTIMISLAHALNYAVGSPFNSDQL
                     RRMIGYNYVEETVEGNVAFGFETGVGPAVSIHGGMAITGDELALVYHHPFAEGKNVFI
                     VIPPTDISSAGTQEFDLLMNKARVLDYRDREIKAYMIMMDLIPALEREDLEKIGNIIW
                     EIEFRGSKRAEVEHHSYAIYRYMSLLREAGLEFVGMSSVGPSIAIITSKGRDEIERIV
                     SNLGLGVAVVTKVDNMGLQITHEGGKSPSGRKKAR"
     gene            1424..>1757
                     /locus_tag="PKL26_07695"
     CDS             1424..>1757
                     /locus_tag="PKL26_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNS83616.1"
                     /translation="MHEFSIAYDIYATARKAAIEHGAREVRSVSVEFGEMAMVSPEQV
                     SFLFETLVEDDPLMKGASLTVSVIPPLSRCTCGYEGTERFVCPRCGGLPELIKGREIL
                     VTNVEIEVN"
BASE COUNT          394 a          478 c          522 g          363 t
ORIGIN      
        1 ataaaaacgc tcgatgcata aacaggaata tttactttga tccgcagtaa tctggtgtca
       61 ggtatatccg tatggcaacc atgaaaatcc ggggcggaga ccttgacctc gtcgaatatg
      121 agttcaactc cttcccccca ggcaagcaga tcaggaccct cggcatagaa cgcccggctt
      181 cactcacgcc tgtcaaagga actgtgaagg tcagggcccc ggccaggatc cacctcaccg
      241 tgctggatat gaacaggttt tcacccgcac gccccggcgg tggcggagtg gggtttgcca
      301 tccagctcta ttgcagcgta gaggccaggt gcattgagag gggcgtagag atcgactaca
      361 accggccagc gatcatccgg cattttgtcg aggtattcag ggagatctcg ggatacgagg
      421 gagggtttgc catcagggcc accgaccacc agcaccagca tgtcggcctt ggttctacga
      481 gcacaatcat gatctccctc gcgcatgccc tcaattatgc cgtgggatct ccgttcaaca
      541 gtgaccagct cagacgaatg atcggatata attacgttga ggagacggtt gaggggaacg
      601 ttgccttcgg tttcgagaca ggtgtggggc cggcggtcag catccacggg ggtatggcca
      661 tcaccggcga cgagctcgcc ctcgtctacc accacccgtt cgcggaagga aagaacgtat
      721 tcatcgtgat cccccctaca gacatctcat ccgcggggac ccaggagttc gacctgctga
      781 tgaacaaggc cagggtgctt gactaccgtg accgcgagat caaggcttac atgatcatga
      841 tggacctgat tcctgctctc gagagagaag atcttgagaa gataggtaat atcatctggg
      901 agatcgagtt ccgtggttcg aagagggcgg aggtggagca ccacagctac gctatctacc
      961 ggtacatgag cctcctgcga gaggccggtc tcgagtttgt cgggatgagc tcggtcgggc
     1021 cctccattgc catcatcacc tccaagggaa gagacgaaat cgagaggatt gtgagtaacc
     1081 tcggcctcgg ggttgcggtc gtcaccaagg ttgacaatat gggcctccag atcacccatg
     1141 agggaggcaa atccccttcc ggcagaaaga aagcccggtg atcacggaaa tggtggagcg
     1201 gcaggggcgt ttgcgcagga cactgcggat atccactccc ccatgacgcc gcactcaacc
     1261 gttttatcag ccatttggtc cctggagtgc gacagggaat gatttatgcc aggcccttcc
     1321 atatatattg gctcatcata tccctgcatt ccctggatga ttcgcccgtt tttcaaggtg
     1381 gcgtgcagct gggtttgccg ggatctccgc gaggcattcc tgcatgcacg aattcagtat
     1441 tgcgtatgat atctatgcca ccgcccggaa ggcagcaatc gagcatggcg cacgcgaggt
     1501 caggtcggtg agcgtggagt tcggagagat ggcaatggtc agccccgagc aggtcagctt
     1561 cctcttcgaa accctcgtcg aggacgaccc gctgatgaag ggtgcgagcc tcacggtgtc
     1621 agttattccg ccactctccc gctgcacatg cgggtacgag gggacggagc gtttcgtatg
     1681 cccccggtgc ggcgggctcc cggagcttat aaagggaaga gagatactgg tgaccaatgt
     1741 cgagatcgag gtgaacg
//
LOCUS       DAOQVJ010000389         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_89423_length_1757_cov_4.175676, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000389 DAOQVJ010000000
VERSION     DAOQVJ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89423_length_1757_cov_4.175676"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKL26_07700"
     CDS             <1..219
                     /locus_tag="PKL26_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83617.1"
                     /translation="EWPYGHCCGGYAGVDRRGEPPPFIAFSTLFHCVDSDHSYHIMTE
                     QQWPYGHCCGEQECHEALLRGFTRDTAP"
     gene            complement(370..1005)
                     /locus_tag="PKL26_07705"
     CDS             complement(370..1005)
                     /locus_tag="PKL26_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS83618.1"
                     /translation="MFTVMVIDDSPFIVDIFVTMLQRGGYQTVAAYGGQEALDTLSTV
                     KPDLILLDIMMEPMDGWETLMNIKNSPDLKNIPVMMLTAKQLTPSEAQEYGIYIEDYI
                     MKPITHKELYEAIEGQLNRRRMIENDIQMATDAGVDKETIDTYRRLRKSIDINTRLLK
                     ILESTYKVSDEKMKTGDEAGLAIRSMSMNIKFQKDQLEQVRDEFFSSAKPA"
     gene            complement(1005..1364)
                     /locus_tag="PKL26_07710"
     CDS             complement(1005..1364)
                     /locus_tag="PKL26_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS83619.1"
                     /translation="MLKEKIKGYIERIKGIEGILDCALISRDGIMLGKSFEGEFNEPW
                     LAAMCATLFASAESTSSILKMPPPGAVSIVIRETSIVVIGAGEKLLIVAVVTDAGRQS
                     PVPRQLEAMAGEIGGSF"
BASE COUNT          368 a          499 c          457 g          433 t
ORIGIN      
        1 gagtggccct atggccactg ctgcgggggg tatgcggggg tggaccggag gggggaaccc
       61 cctccgttca ttgcgttcag tacacttttc cactgcgttg attcagacca ttcgtatcac
      121 ataatgacag agcagcagtg gccctatggc cactgttgcg gggagcagga gtgccatgag
      181 gcgctcctgc gggggttcac gcgggatact gctccatgag gattttctca ctcgttgact
      241 atacggccgg atatccctgt catagggaga gagtaaaaat gttccttctg ttcagtcatt
      301 ccgcgttcta ttgcgttaaa gggaggatcc gcaatcccat cccaccaaaa aaagagaaac
      361 ttccgacctt tacgccggct tcgcgctgga aaaaaactcg tctcgcacct gctcgagctg
      421 gtctttctgg aacttgatgt tcatgctcat gctccggatg gcaagccctg cctcgtcccc
      481 cgtcttcatc ttctcgtcac tcactttgta cgtgctctca agtatcttga ggaggcgggt
      541 gttgatgtcg atgctcttcc gaaggcgcct gtacgtgtcg atggtctcct tgtccacccc
      601 tgcatcggtg gccatctgga tatcgttctc gatcatcctc ctccggttga gctgcccctc
      661 gatcgcttca tagagctcct tgtgggtgat gggcttcatg atgtaatcct cgatgtagat
      721 gccgtactcc tgcgcctcgc tcggggtgag ctgctttgca gtgagcatca ttacggggat
      781 attcttcaga tcgggggaat tcttgatgtt catgagggtc tcccagccat ccatcggctc
      841 catcatgatg tcaaggagga tcaggtcggg cttgacggtg gagagggtat cgagcgcctc
      901 ctgtccacca taggcggcca cggtctggta tccccctctc tgcagcatgg tcacgaagat
      961 atcgacaata aacgggctgt catcgattac catcaccgtg aacattaaaa tgaccccccg
     1021 atctccccgg ccatggcttc gagctgccgt ggtacagggg attgcctgcc cgcatcggtg
     1081 acaaccgcga cgataaggag tttctcccct gccccgatta ccacgattga ggtctccctg
     1141 atcacgatgg ataccgcacc gggaggcggc attttgagga tcgacgatgt cgactcggcg
     1201 gaggcaaaga gcgtggcaca catcgcggca agccacggtt cgttgaactc tccttcaaac
     1261 gacttgccga gcatgatccc atccctcgag atgagcgcac agtcaagaat tccttcgatc
     1321 cccttaatcc tctcgatata ccccttaatc ttctccttca gcatatgacc tcacccttca
     1381 tcatggtcgc cctgaatgac ggaagtgcac acaggttatt cattctcgct atagatattc
     1441 ctgatcaaag atcccccatg tggcgcaggg tcgcaggaca gcctgccgga ttccaccacc
     1501 cccaggtcat ggccggaaga cccaggaatt accgtttctt tcacccgagc ggagtccggc
     1561 cggattggat gccatgggag atgggatgtt ttattacata tcccctctat acataatata
     1621 gcccaaaata cacaatattt gggttcagaa atgcaaaata acagtcttat ggctgtaaaa
     1681 tttgcaagga caatttcccg cccggggcca tagggtagcc tggccatcct aggagactgg
     1741 gggtcttctg acctgcg
//
LOCUS       DAOQVJ010000390         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_89502_length_1756_cov_5.487360, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000390 DAOQVJ010000000
VERSION     DAOQVJ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89502_length_1756_cov_5.487360"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKL26_07715"
     CDS             complement(<1..329)
                     /locus_tag="PKL26_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS83620.1"
                     /translation="MSSNPFKEENAQPCIDEVRATPGVAACALVSGEGAILGKYFREG
                     NRSSSLFAVMCATVLASAEAACGSVHLQSPSMVTITAPDATILIVGARDGTLITAVID
                     KPADLPT"
     gene            884..1693
                     /gene="minD"
                     /locus_tag="PKL26_07720"
     CDS             884..1693
                     /gene="minD"
                     /locus_tag="PKL26_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HNS83621.1"
                     /translation="MIRAYTIASGKGGTGKTTVVANLGTALAQYGRETCIVDTDIGMA
                     NLGLVLGLTRTPVTLHEVLAGKAPIQDAIYEGPYGLKIVPSGPSLEGFQNADPARLKD
                     VMTDLTDRCDYLFFDAPAGISTDAIMPLSVADEVLLVVNPELSSLVDALKIRILTEMV
                     GGTVMGTLLNRMTMESTDVSRGKIEKALGVPVIDIIPEDANVRRAAAARMPVVAKYPQ
                     SESSKAFRRIAATLAGLPIEEEPEKVREGFIERFARALFRRDSECSTSMDS"
BASE COUNT          387 a          489 c          535 g          345 t
ORIGIN      
        1 attgttggga ggtctgcagg tttatctatt accgctgtga tcagggtgcc gtctctcgca
       61 cccacgataa ggatagtagc gtcaggtgcg gtgatggtga ccatggaagg gctctgcagg
      121 tggacgctgc cgcatgcagc ctccgcagat gcgagaaccg ttgcacacat caccgcgaag
      181 agggatgatg agcggttgcc ctcccggaaa tatttaccca ggatcgcacc ttcaccggat
      241 acgagggcgc atgcggcgac accaggtgtt gcacgaacct catcgatgca cggttgagca
      301 ttctcctcct tgaagggatt ggaactcata cgactgaccc cggtatatct ccatggagat
      361 atctgcgccg catcatatat gaatatcgaa tcagtatcgc ctcgaaggtg ccgctgtcct
      421 gtgttttgtc aggattcaga gagcaccgcg accgcggggg agcagggcgc tgtcgcaaca
      481 tcctcgcggg cacggggttg cggcgttcca gttctacgag cagatagccg agtcgcaaca
      541 tcccgcgggc acgggggctg ttccaggagg ttgactcacg gcactccacg gtgcacgact
      601 ttgccgatcc ccgcctttat gcgccggtgc tggcaaccga gcgtgaacga cagggtacca
      661 tgcgaaagcc acggcgccgt agcgatccat aagaccccgg gattgaggcg atccggtatg
      721 tccgtagggc aggaacatga atccgctccc cggcgccgat caaccggggg cctcgcgagg
      781 gagagaatac ctggtataaa agcagactct cctttttcca cgaagagacc gaacgaataa
      841 gtttaaatta gatcactgca acctatccct gagacgattt tagatgatca gggcctacac
      901 aatcgcctcc ggaaagggag ggaccggaaa gacgaccgtg gtcgcgaatc tcggaacagc
      961 actggcccag tacggcaggg agacctgtat cgtcgatacg gatataggta tggccaacct
     1021 cgggctggtg ctcggactca ccaggacgcc ggtcacgctc catgaggttc ttgcagggaa
     1081 agcaccgatc caggatgcca tttatgaggg gccctacggc ctcaagatcg tcccgagcgg
     1141 cccttcactt gagggtttcc agaacgcgga tcctgcgcgg ctcaaggacg tgatgaccga
     1201 tctgaccgac cgctgcgact acctcttctt cgacgcacct gccggcatca gcaccgatgc
     1261 gatcatgccc ctcagcgtgg cggacgaggt gctgctggtg gtgaatcccg aactctcctc
     1321 gctcgtggat gccttgaaga tcaggatcct gacggagatg gtcggcggca ccgtcatggg
     1381 gacgctcctc aaccggatga ccatggagag caccgatgtg agtcggggga agatcgagaa
     1441 ggccctgggc gtgccggtca tcgatatcat acccgaggac gcgaacgtga gaagggcagc
     1501 ggcggcgagg atgccggtcg tcgcgaagta cccgcagtcc gagtcatcaa aagcattccg
     1561 gagaattgcg gccacgctgg cgggtctccc catcgaggaa gagcccgaaa aggtcaggga
     1621 aggatttatc gagcggtttg cccgcgcact attcaggagg gattcagaat gttcgacatc
     1681 aatggactca tgatagtttt actctcggtg atcatcatct gcctgttgtt tatcatgtac
     1741 gtcatgtacg tgcgca
//
LOCUS       DAOQVJ010000391         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_89805_length_1753_cov_2.565371, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000391 DAOQVJ010000000
VERSION     DAOQVJ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89805_length_1753_cov_2.565371"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKL26_07725"
     CDS             <1..630
                     /locus_tag="PKL26_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HNS83622.1"
                     /translation="IVVIVAGVGCFVGLVANVVEHVAFRKERARRRQKTMMLVGIFFT
                     QVGTSLLATCARGDLGRQDLSRLLSGPKFADSPASAGITDALASRSYHLDCRELDLAT
                     LRDALGEKIDVLSVLLQNPDLDEHSKFTDLLQALFHLWQELSHREVLCGLPGADTAHL
                     SGDLNRVYGLLVLEWVVYLGHLKRHYPYLYSLALRTNPFDEKASVVIRE"
     gene            784..>1753
                     /locus_tag="PKL26_07730"
     CDS             784..>1753
                     /locus_tag="PKL26_07730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS83623.1"
                     /translation="MKTISFVDLDLPEPLMRAVLDQGFEEPTPIQSLAIPVIREHHDM
                     VGQAHTGTGKTAAYGIPLLEQVDQKVPEVQAIVLCPTRELAIQVSEELRKLARHLRGI
                     TILPVYGGQPIERQIAVLKKGVQVVIATPGRLIDHLDRRTVSLSGIRLVVLDEADEML
                     DMGFRDDIEIILKRTPAERQTVLFSATMSKEILALAQKYLKDPRMVKVVHGRLAVPAI
                     EQRYFEVRESDKVEVISRLLDFYNPKLSLVFCNTKRKVDEVSGQLQARGYLAEALHGD
                     MDQKERERVMSRFRTGASDILVATDVAARGIDVEEVGVVFNYDVPQD"
BASE COUNT          332 a          604 c          520 g          297 t
ORIGIN      
        1 atcgtcgtga tcgtcgcagg cgtagggtgc ttcgttggcc ttgtcgcaaa cgtggtcgag
       61 cacgtggcct tccggaagga acgggcccgc cggcggcaga agacgatgat gctggtcggg
      121 atcttcttca cgcaggtggg cacttcgctc cttgcgacct gtgcacgggg tgacctgggc
      181 cgccaggacc tctcccgcct actgtcaggg ccaaaattcg cagactcccc cgcgtccgcc
      241 gggatcaccg acgcgctcgc gtcccggtca taccatctcg actgccggga actcgacctc
      301 gcaaccctcc gggacgccct gggggagaaa atcgacgtcc tttccgtcct cctccagaac
      361 cccgacctcg acgagcactc gaagttcacc gacctgctcc aggctttgtt ccacctctgg
      421 caggaactct cgcaccggga agtactctgc ggtctcccgg gggccgacac ggcgcacctc
      481 tccggagacc tgaaccgggt ctacggcctc ctggtcctcg aatgggtcgt gtacctgggc
      541 cacctgaagc gccactaccc ctacctctac tcccttgcgc tccgcaccaa cccgttcgac
      601 gagaaggctt cggtggttat cagggagtag cactccatcg gggtggagga gagataccca
      661 tcgcaaatgc ctataaaggg taagggggca ttattcctgc agcacgatcc gggggaccat
      721 gcagaccagt gaatactgcg cgggctcccc gggagaatgc ccggaacgct acggaacatg
      781 tacatgaaaa ccatctcatt tgtcgatctc gacctccccg aaccgctcat gcgggcagtc
      841 ctggaccagg ggttcgagga gcccaccccc atccagtcgc tcgccatccc cgtcatccgc
      901 gaacaccacg acatggtggg ccaggcccac accgggaccg ggaagaccgc cgcctacggc
      961 atcccgctcc tcgagcaggt ggaccagaaa gtcccggagg tccaggccat cgtcctctgc
     1021 cccacgaggg agcttgccat ccaggtctcg gaggagttga ggaagctcgc ccggcacctg
     1081 cgcgggatca ccattctgcc cgtctacggg ggccagccca tcgagcgcca gatcgctgta
     1141 ctcaaaaaag gtgtgcaggt cgtcatcgcc acccccggcc gcctgatcga ccacctcgac
     1201 cgccgcaccg tcagcctctc ggggatccgg ctcgtcgtgc tcgacgaggc cgacgagatg
     1261 ctggacatgg gctttcggga cgacatcgag attatcctga aacgcacccc ggccgaacgc
     1321 cagactgtcc tcttctcggc caccatgtca aaggagatcc tcgcgctcgc ccagaagtat
     1381 ttaaaagacc cgcgcatggt caaggtggtg cacggccgcc tggcggtccc cgccatcgag
     1441 cagcgctact tcgaggtgcg ggagtcggac aaggtcgagg tcatctcccg gctgcttgac
     1501 ttctacaacc caaagctctc gctcgtcttc tgcaacacga aaaggaaggt ggacgaggtc
     1561 agcggccagc tccaggcccg cgggtacctc gccgaggcgc tccacggcga catggaccag
     1621 aaggaacgag agcgggtgat gtcccggttt cggaccggcg cctccgacat cctggtcgca
     1681 accgatgtcg ccgcacgcgg gatcgacgtc gaggaggtcg gggtggtctt caactacgac
     1741 gtcccccagg acc
//
LOCUS       DAOQVJ010000392         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_89950_length_1751_cov_3.433373, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000392 DAOQVJ010000000
VERSION     DAOQVJ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89950_length_1751_cov_3.433373"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..682
                     /gene="ruvA"
                     /locus_tag="PKL26_07735"
     CDS             86..682
                     /gene="ruvA"
                     /locus_tag="PKL26_07735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNS83624.1"
                     /translation="MIARLRGRVVAGGDGFVILETGGVGFQVFMPRPLADEAARSPAE
                     VVIYTHLVARDDTMALYGFLAPGELEMFRLLITVNRVGPALATAILSQMTVSDLAAAV
                     IGGDEKALTRVSGVGQKNAARIILELKDRMKKKAALLVEGRDTGADPTRSDAESALLA
                     LGFSPRDSRDAVSETLAGMDCPTVQDVIKASLRRLRER"
     gene            689..1681
                     /gene="ruvB"
                     /locus_tag="PKL26_07740"
     CDS             689..1681
                     /gene="ruvB"
                     /locus_tag="PKL26_07740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNS83625.1"
                     /translation="MDERVVSPVPEEGEVDDPAIRPDRFSDFIGQEALKETLRIAIEA
                     ARRRGEPLDHTLFSGPPGLGKTTLAHIIAREMDSSIHCTSGPVLEKPGDLAAILTLLK
                     RGDVLFIDEIHRLHTVIEEILYPAMEDFFIDVIIGEGPSARSIKITLEPFTLVGATTR
                     IGLLGSPFRDRFGLISRLSLYGHEDLARIIERSAAIMGIPILPDGSMEIARRSRGTPR
                     IANRLLRRVGDYALVRGGGVVDRDVADRALCMLGVDSLGLDELDRRIIQVIVQEFGGG
                     PVGVKTIAIAVGEDTRTIEEVHEPYLIQAGFIKRTPQGREATPAARGHLTRQWW"
BASE COUNT          334 a          533 c          576 g          308 t
ORIGIN      
        1 gtctcccgga catcccgagg cctgacgatg ttgccgacgg tctctcgatc gccctctgcc
       61 atatccatac cgtgagggat acccgatgat tgcaaggctt cgcggccgcg tcgtcgcggg
      121 aggcgacggg tttgtgatcc tggagaccgg aggggtggga ttccaggtat tcatgccgcg
      181 gcccctggca gatgaggcag caaggtcccc tgccgaggtg gtgatctaca cccacctggt
      241 cgcgagggac gatacgatgg cgctgtatgg cttcctcgca ccgggggagc tcgagatgtt
      301 ccggctgctg atcactgtca accgggtcgg ccccgcgctc gcgactgcca tcctctcgca
      361 gatgacagtc tcggaccttg cggccgccgt gataggaggg gacgagaagg ccctgacccg
      421 ggtatctggc gtagggcaga agaatgccgc acggatcatc ctcgagttga aggaccggat
      481 gaagaagaag gcagcgctgc tcgtcgaggg acgtgatacc ggggctgatc ccacaaggag
      541 cgacgccgag agtgccctcc ttgcacttgg tttctccccc cgggactcaa gagatgcggt
      601 ctcggagacg ctcgccggga tggactgccc gaccgttcag gacgtgatca aggcatccct
      661 tcgcaggctg cgggagcggt gacccgggat ggatgagcgc gtggtctcgc ccgtgccgga
      721 agaaggcgag gtcgatgacc cggcaatccg cccggaccgc ttttcggact ttatcggcca
      781 ggaagccttg aaagagaccc tccgcattgc gatcgaggcg gccaggaggc gtggagaacc
      841 actggaccac accctctttt caggtcctcc gggccttggg aagaccaccc ttgcgcacat
      901 catcgcccgg gagatggact cctccatcca ctgcaccagc gggccggtgc tcgaaaaacc
      961 cggcgacctc gcggcaatcc tcaccctcct gaagcgggga gacgtcctct tcatcgacga
     1021 gatccaccgc ctgcacacgg tgatagagga gattctgtac cccgcgatgg aagacttctt
     1081 catcgacgtg atcatagggg agggcccgag cgcacggtcc ataaagatca cactcgaacc
     1141 tttcacgctt gtgggggcaa ccacgcggat cgggctgctc ggttccccct tcagggaccg
     1201 gttcggcctg atctcccggc tctccctcta tggccacgag gaccttgcca ggatcatcga
     1261 acggagcgcc gcgatcatgg ggatccccat cctccccgac ggcagcatgg agatcgcaag
     1321 gaggagccgg ggcacgccac gaatcgccaa ccggctcctc cggagggtgg gggactatgc
     1381 ccttgtgcgg ggagggggag tggtggaccg tgacgtggcc gaccgggcgc tctgcatgct
     1441 gggggtggac agcttgggcc tcgacgagct ggaccgccgt atcatccagg tgatcgtcca
     1501 ggagtttggc ggcggccccg tgggggtgaa gacgattgcc atcgccgtcg gcgaggacac
     1561 gaggactatc gaggaggtcc atgaacccta cctcatccag gcaggtttca tcaagcgcac
     1621 cccccagggg agagaggcaa cacctgccgc gagggggcat ctcacccggc agtggtggtg
     1681 atctgcgtgt attcagtcgc attttggggg atattataaa tacccggaga gattatcgaa
     1741 aaatatacca g
//
LOCUS       DAOQVJ010000393         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_90352_length_1746_cov_3.929036, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000393 DAOQVJ010000000
VERSION     DAOQVJ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90352_length_1746_cov_3.929036"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..601
                     /gene="rnhB"
                     /locus_tag="PKL26_07745"
     CDS             2..601
                     /gene="rnhB"
                     /locus_tag="PKL26_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNS83626.1"
                     /translation="MVAGVACRRTQDLAKLGVKDSKALSPARRETLAAELKASFPVEV
                     LVISPGEIDSRGMTMNALMAKSHARVIDALHPAVAVVDACDVVAARYGAMVSSHLDRP
                     CRVVSRHHADQHHPAVSAASIVAKVERDHAIEHLKREFGEIGSGYPADPFTVRFLEEY
                     VSTNQGPPPIARRSWETVRVLLARHEQASLFDFPGGDTE"
     gene            661..>1746
                     /locus_tag="PKL26_07750"
     CDS             661..>1746
                     /locus_tag="PKL26_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNS83627.1"
                     /translation="MHWLFYVLLAVAAYAIVAGYIYTRKAAYERERADRIAFPEMAEG
                     RPERSFWDAIVFYGPIIAIKTERVAFFDRFTRFSTLLRLYASFGVVMVVIISVGIALI
                     LFVSVQFTLLLRPEPTGIYAPQNILLIPGLNEYVPSTLAVWFAFVLTIAIHEFGHGIL
                     CRVEKIGVRSMGALIAVIPIGFFVEPDEQELEKSRGLPKVRMFGAGITNNIVIGAICF
                     VALFLLAGMAVPPSGPVLAGVYQNYSAWEAGVPTPSLITGINGVPVGTREDVSALLNG
                     TRPGDALALTVEYQGQQSSYALTLSEWPKELGERDSGFMGVHYYDGAMIMDQVTQGIS
                     VPGFFRFLTIPFDPSMGGQALRMLAFET"
BASE COUNT          311 a          548 c          545 g          342 t
ORIGIN      
        1 cgtggtggcg ggcgttgcct gccggaggac gcaggacctc gcgaaactcg gggtgaagga
       61 ctcgaaggcc ctgtcgcctg cccgtcgtga aacgcttgcc gcggaattga aggcctcgtt
      121 cccggtggag gtcctggtga tctctcccgg ggagatcgac tcgcggggca tgaccatgaa
      181 cgccctcatg gcaaaatccc atgcacgggt gattgacgca ctccaccccg cggtcgccgt
      241 cgtggacgcc tgcgacgtgg tggccgcccg gtacggggcc atggtctcgt ctcacctgga
      301 ccgcccctgc agggtggtgt cccggcacca cgccgaccag caccatcccg cggtctctgc
      361 ggcaagcatc gtggcaaagg tagagcggga ccacgcaatc gagcacctca aacgagagtt
      421 cggggagatc ggaagcggct accccgcgga ccccttcacc gtccggttcc tcgaagagta
      481 tgtcagcacg aaccagggac ccccgcccat tgcgcggagg agctgggaga cagtcagggt
      541 cctcctcgcc aggcacgaac aggcaagtct ttttgatttt ccgggcggag acacggaata
      601 agcccgtgat tcctgtcatt tcgcacaggt ttatgtagat ttcccgcgta atatacagga
      661 atgcactggc tcttttacgt gctcctggcg gtagcggcgt atgccattgt agccggctat
      721 atttatacca ggaaggccgc ctacgaacgt gagagagccg accggatcgc cttcccggag
      781 atggcagaag ggaggcctga acggtccttc tgggatgcaa tcgtcttcta cggcccgatc
      841 attgccataa agaccgagcg ggtggcgttc ttcgaccgtt tcacccggtt ctccacgctg
      901 ctccggctct acgcctcgtt cggggtggtc atggtggtga tcatctctgt cgggattgca
      961 ctcatcctct ttgtctcggt ccagttcacc ctcctcctcc gccccgagcc caccggcatc
     1021 tacgcaccgc agaatatcct gctgatacca gggctcaacg agtacgtccc ctcaaccctg
     1081 gcggtatggt tcgcgtttgt cctgaccatt gcgatccacg agttcgggca cgggatcctc
     1141 tgccgggtgg agaagatcgg ggtgcggagc atgggtgcgc tgatcgcggt catcccgatc
     1201 gggttcttcg tcgagcccga cgagcaggaa ctcgagaaaa gccgggggct gccgaaagtc
     1261 cgcatgttcg gggcgggaat cacgaacaac atcgtgatcg gggcgatttg cttcgtggcg
     1321 cttttcctcc tggcgggaat ggcggtgccc ccctcggggc cggtgctcgc aggagtctac
     1381 cagaactatt ccgcgtggga agcgggagtt cccactccct ccctcatcac cggcatcaac
     1441 ggggtcccgg tagggacccg cgaggacgtc tcggctctcc tgaacggcac ccggccgggc
     1501 gatgccctcg ccctgacagt ggaataccag ggacagcagt cgagttacgc cctcaccctc
     1561 tcggaatggc caaaagagct cggggagagg gactcaggct tcatgggggt gcattactac
     1621 gacggcgcca tgattatgga ccaggtgacg cagggcatct cggtcccagg gttcttccgg
     1681 ttcctgacga tccccttcga cccttccatg ggaggccagg cgctccgaat gctggccttc
     1741 gagact
//
LOCUS       DAOQVJ010000394         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_90541_length_1744_cov_3.676140, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000394 DAOQVJ010000000
VERSION     DAOQVJ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90541_length_1744_cov_3.676140"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PKL26_07755"
     CDS             complement(<1..982)
                     /locus_tag="PKL26_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNS83628.1"
                     /translation="MPECAGPLWPCGLTGAAACLSGVKGMGVIVHGSAGCYFYPATTL
                     HTPIHCTSLTDHDLILGTGDALIRTIDSLQGDYSRLAVLCTCTPAIIGEDIRAVIDER
                     YGDSTRVLVLDAPGFAGEYEAGFLAALSAMRLSPGGGREDSVNIDGLSLMDPFWRGNL
                     QEIQRLTRLAGAECGATLCAGGFSCDSEISSWTIHANPDIPSCLGESLGTMFGISGMK
                     KAVLAFEDRFGRNGGVLSEFVLPDAEERIISACDKYQRRFDPPVAALFGQSASMQAVA
                     AMLSEYLCAEIACIGSRNTPSHGRFPVEMARDYATVSEMLEHERPDLVLGS"
     gene            complement(975..>1744)
                     /locus_tag="PKL26_07760"
     CDS             complement(975..>1744)
                     /locus_tag="PKL26_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNS83629.1"
                     /translation="RKPELICVLSTCVAATIGDDVDAVCRHHGEVPVVHIPTAGFLGG
                     GFLAGHIQALLSMSRFAEPREHGLSVNLIGEKTLEYEVDEHYREVVRLLSLMGVPVNV
                     RFVCGVSLGQIQALGEASLNVLRDPSMVPLGEHLFRELKIPYIDSFPIGVDGTLRFLE
                     RTGRYLSLDSSEAIATEEALQKDLIRAFAPLRGQRITFNGSPMAELHLQYGYELAEAL
                     GLEVREGGCAVPLPDPFPVGTSGIRRLLQRWRRTCHA"
BASE COUNT          377 a          551 c          495 g          321 t
ORIGIN      
        1 aagacccaag gaccaggtca gggcgctcat gttcaagcat ctcgctgact gtcgcatagt
       61 cccttgccat ctctacaggg aaccgcccgt gggaaggggt atttctggaa ccgatgcacg
      121 cgatctctgc acacaggtac tccgacagca tggctgcaac tgcctgcatc gacgcggact
      181 ggccgaagag ggctgcaacg ggggggtcaa accttcgctg gtacttgtcg catgcagaaa
      241 tgatccgttc ctcggcatca ggaagaacga actcggagag cacccctccg tttctcccga
      301 acctgtcttc gaacgcgagg acggcctttt tcatcccgga tatcccgaac atcgtgccca
      361 gagattctcc aaggcatgac gggatgtcag ggtttgcatg gatggtccag gaagagatct
      421 ccgaatcgca cgagaacccg cctgcacaca gcgttgcacc gcattccgct ccggcaagcc
      481 tggtcaggcg ctggatctcc tgcaggttcc ccctccagaa ggggtccatc agcgagagcc
      541 cgtcaatgtt gaccgagtcc tctcggcccc cgcccggaga taggcgcatt gcagagagcg
      601 ctgccagaaa acccgcctcg tactcaccgg caaatcccgg ggcatcgagc acgaggaccc
      661 gggtggaatc tccgtacctc tcatcgatca ctgcacggat gtcctcaccg atgatcgccg
      721 gtgtgcaggt gcacaggacc gcaagccgcg aataatcccc ctgcagcgag tcgatggtcc
      781 ggatcagcgc gtcgcccgtc ccgaggatga ggtcatggtc cgtgagcgac gtgcagtgta
      841 tcggggtatg aagggtggtt gccgggtaaa aataacatcc cgcggacccg tggacaatca
      901 cacccatccc ctttaccccc gaaaggcagg cagctgcccc cgtcagcccg catggccata
      961 gcggccctgc acactcaggc atggcacgtt ctcctccatc gctgcagcag cctccggata
     1021 ccgctggttc cgacagggaa tggatcaggg agcgggacgg cgcatccgcc ctcgcgtacc
     1081 tcgaggccga gagcctccgc tagctcgtac ccatattgca gatgcagttc agccatcggt
     1141 gatccattga aggtgatcct ctgtcccctc aggggagcaa aagcccttat caggtccttc
     1201 tgaagagctt cttcggttgc aatggcctcc gatgaatcaa gggaaaggta tctgcccgtg
     1261 cgctcgagga accggagcgt gccatcgacc ccgatgggaa acgagtcgat ataggggatt
     1321 ttcaactccc tgaaaagatg ctctcctaac gggaccatcg aagggtctcg aaggacattc
     1381 agcgacgcct ccccgagggc ttgtatctgg ccgagcgaaa caccgcatac gaacctgaca
     1441 ttcaccggga ctcccatgag cgagaggagg cggacaacct cgcggtaatg ctcgtccacc
     1501 tcgtactcaa gtgtcttctc gccgatgagg ttgacggaca gcccatgctc ccgcggctcc
     1561 gcgaacctgg acatcgagag gagtgcctgg atgtgtccgg caagaaatcc cccaccgagg
     1621 aatcccgccg tgggaatatg caccacagga acctcaccat ggtgccggca tactgcatcc
     1681 acgtcatccc ctatcgttgc agcgacgcag gtggagagga cacagatcag ttcaggtttc
     1741 cttt
//
LOCUS       DAOQVJ010000395         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_91212_length_1736_cov_2.407496, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000395 DAOQVJ010000000
VERSION     DAOQVJ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91212_length_1736_cov_2.407496"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /gene="glmS"
                     /locus_tag="PKL26_07765"
     CDS             <1..1670
                     /gene="glmS"
                     /locus_tag="PKL26_07765"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNS83630.1"
                     /translation="EYRGYDSFRIAVSNQGITVRKREGKISLGGADLARVKGTMGIGH
                     TRWATHGVPNDQNAHPHLDCQGTIAVVHNGIIENYAALKRELQDRGHEFRSETDTEVI
                     PHLIEEAFRGDLAAAVGEIIPRLEGSYALLVIAAGCTEMVAVRQKSPLVLGVGDRAFL
                     AASDVTPILEHTERVIFLEDGDIATLSAAGIEIQHGGTGVKRPVQRIDWSLAEVKKGG
                     FPHYMQKEIYEQPEVFSLTTRALRGEETPAVLQGIRGVTLVACGTSFNAALLCRYFIE
                     EYCGIPARVELASEFKYYMPPGDDLVVGVSQSGETADTLAAIEKARMHNHPTLAVTNV
                     LGSTITRLADHTLYMRAGPEISVAATKSFIAELAVFFHLVDRMSEGALESSLSTGHLA
                     IGEALLQDLSPAVELCRSAHSMFFVGRGPFYPLALEGALKMKEISYIHAEGYAAGEIK
                     HGPFALLSHETPVVALCPPGQAFPVMFSNIREMKARGTPMIVIGAGGNQDLQEVSDVF
                     IPLPEGGLVSHVLASTALLQLLAYHTAVALGRDVDQPRNLAKSVTVE"
BASE COUNT          353 a          525 c          551 g          307 t
ORIGIN      
        1 tcgagtaccg gggatacgac tcgtttcgaa tcgcggtgtc aaaccagggc atcacggtaa
       61 ggaagaggga agggaagatc tcactcggag gtgcggacct tgccagggtg aagggaacga
      121 tggggatcgg ccacacccgg tgggccacac acggggtccc gaatgaccag aatgcccacc
      181 ctcacctcga ctgccagggt accatcgccg tggtccacaa cgggatcatc gagaattacg
      241 cagccctgaa gcgggaactc caggaccgcg ggcacgagtt ccgctccgag accgacacgg
      301 aggtcattcc ccacctcatt gaagaggcat tccggggaga cctcgccgcg gcggtggggg
      361 aaatcatccc caggctggag ggcagctatg cccttctcgt gattgcggcc gggtgcacgg
      421 agatggtcgc ggtacggcag aagagcccgc tcgtgctggg ggtgggtgac cgggcgttcc
      481 tggcggcatc cgacgtcact cccattctcg agcataccga gcgggtcatc ttcctcgagg
      541 acggggacat cgccacactc tcggcagcgg ggatcgagat acagcacggg gggaccggtg
      601 taaagaggcc ggtccagagg atcgactggt ctcttgccga ggtgaagaag ggagggttcc
      661 cccattacat gcagaaggag atctacgagc agccggaggt cttctccctc actaccaggg
      721 cccttcgcgg ggaggagacg cccgcagtcc tgcagggtat ccggggagtc actctcgtgg
      781 cgtgcgggac gtccttcaac gccgcgctcc tctgccgtta cttcatcgag gaatactgcg
      841 ggatcccggc acgcgttgaa cttgcatccg agttcaagta ttatatgcca cccggggatg
      901 acctcgtcgt gggggtgagc cagtcaggcg agaccgcgga tacccttgcc gcaattgaga
      961 aggcccgcat gcacaaccac cccactctcg ccgtgacaaa cgtcctcggg agtacgatta
     1021 ccaggctcgc ggaccacacc ctctacatga gggcaggccc cgagatcagc gtcgcggcca
     1081 caaagtcgtt catcgcggag ctcgcggttt ttttccactt ggtcgaccgc atgagcgaag
     1141 gagcgcttga atcgtctctc tcaaccggtc accttgcaat cggggaggca ttgctccagg
     1201 acctctcgcc cgcggttgaa ctatgcagga gtgcccactc catgttcttc gtgggacgag
     1261 gcccgttcta tccactggcg cttgaaggcg ccctgaagat gaaagagatt agctacatcc
     1321 acgccgaggg gtacgcggca ggggagatca agcacgggcc cttcgccctc ctttcccacg
     1381 agacgccggt ggtcgcgctc tgcccgccgg gccaggcatt ccccgtgatg ttctccaata
     1441 tcagggagat gaaagccagg gggaccccga tgatcgtgat cggtgcgggg gggaaccagg
     1501 acctccagga ggtatccgac gtgttcatcc cgctcccgga agggggcctg gtctcacacg
     1561 tgctcgcgtc gaccgcactc ctgcagctcc tcgcgtacca caccgcggtc gcgctgggaa
     1621 gggacgtgga ccagccgagg aacctggcaa agagcgtgac ggtggagtga attatcttaa
     1681 aaatattccc tgtggtaaga ctccaggcgt tcaacagaat gaatatctga acctct
//
LOCUS       DAOQVJ010000396         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_91507_length_1732_cov_5.426953, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000396 DAOQVJ010000000
VERSION     DAOQVJ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91507_length_1732_cov_5.426953"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..1357
                     /locus_tag="PKL26_07770"
     CDS             470..1357
                     /locus_tag="PKL26_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNS83631.1"
                     /translation="MRWRTERRSTNIEDRRSSGIPMGRSSGIPGGKATGIGGLALILV
                     LVLAVAMGVDPGQIISGTDYASDSYGYQSPLTDTQQDELADHVALVLGDTESTWATIF
                     EDQLGKTYREPKLVLYTGYVDSACGQASAAVGPFYCPADEKVYLDLDFFYALQQEYHA
                     PGDFAQAYVISHEIGHHVQNLLGTIGYVDRKTRYLSDADANELSVALELQADCYAGVW
                     AKQAMYDDLILESGDLEEAMNAASAVGDDTLQMQYQGYVVPDSFTHGTSAQRVSWFRR
                     GMDAGLVRDCNCPGLPVIG"
     gene            complement(1442..>1732)
                     /locus_tag="PKL26_07775"
     CDS             complement(1442..>1732)
                     /locus_tag="PKL26_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNS83632.1"
                     /translation="GLLGSFVSGSETSSNVMFYGILQKATNVLSLDFEKVYAAHMVAG
                     GIASGIAPAKIINAAAVIDQASIEGEVIKKSAVVVILLALLTSALLVLSLVI"
BASE COUNT          398 a          471 c          486 g          377 t
ORIGIN      
        1 ggcaacgacc tcatccaatg cgcttacggg catccggcat cctttgaccc tctcgttgac
       61 gtggcgatgc tcgggttttt cttcgtgatg ttccagctca ttgcccaccg gctctatgcg
      121 catttcaacg attgaggaac gtcccatccg ccgcaccgtg gtcacgacag ccgattttct
      181 ttactctcca ttgagaaggc gcgtcacatt tcagcacctt caccaactat atctcaagga
      241 ttacgctctc acatgaggta tggcgatgga tgagggttac catgacctat gtcgccctgc
      301 ctattccgga aaatattttt cccaatccct cacaatcggg aagtgcagga tcacctgcga
      361 tggattccag gaaccatttt atcaatcaac acaggacaat cgggtatgag gggaacgcaa
      421 aggaaggttc cggattcatg gcagggacta gataaggaat gtgagagata tgaggtggcg
      481 gacggaacga cggagcacca atattgagga ccggagatcc tcggggattc ctatgggaag
      541 atcctcgggg attccagggg gcaaagcgac aggaatcggg gggctcgcgc tcatcctggt
      601 tcttgtcctt gcagtggcca tgggtgtcga ccctggccag atcatcagcg gaactgatta
      661 cgcttcggac tcgtatggat accagtcccc gctcacagac acacaacagg acgaacttgc
      721 tgatcacgtg gcactcgtgc tcggcgatac ggagagtaca tgggccacca tcttcgagga
      781 ccagcttggg aaaacttaca gggaaccaaa acttgtcctc tacacgggat atgtagattc
      841 agcatgcgga caggcgagcg cagcggtggg gccattctac tgccccgccg atgagaaggt
      901 gtacctcgac cttgatttct tctatgcgct gcaacaggaa taccatgccc ccggcgattt
      961 tgcccaggca tatgtgatat cccatgagat tggccaccac gtccagaatc ttcttgggac
     1021 catcgggtac gtggacagga aaacacgcta tctctccgac gctgatgcga acgagctctc
     1081 tgtggcgctc gagcttcagg ccgactgcta tgcgggtgtg tgggccaagc aggcaatgta
     1141 cgatgacctg atccttgaat caggcgatct cgaagaggcc atgaacgcgg cgagtgcggt
     1201 cggtgatgac actctccaga tgcagtacca gggatacgtc gtgccggaca gtttcaccca
     1261 tggtacttcc gcacagcggg tatcgtggtt cagaagaggg atggacgccg gtctagtgag
     1321 ggactgcaac tgtcccggcc tcccagtcat cgggtgagga tggcaaaggg aacaatccct
     1381 tccatcctgc ctgtctctta tcggccttcg catgggacgg cggccgggat tctcaatccc
     1441 ttcagatgac aagtgaaagg accaggagtg cagatgtgag caatgcgagg agaataacca
     1501 cgacggccga tttcttgatc acttctccct cgatcgaggc ctggtcaatc actgctgcgg
     1561 cattgatgat cttggccggg gcgatcccgg atgcgatgcc accggccacc atgtgggcgg
     1621 catagacttt ctcaaagtcg agcgatagta cgttcgttgc cttctgcagg atcccataga
     1681 acatcacgtt cgaagaggtc tcgctcccgg aaacgaaaga tccgagcaat cc
//
LOCUS       DAOQVJ010000397         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_91882_length_1728_cov_4.838613, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000397 DAOQVJ010000000
VERSION     DAOQVJ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91882_length_1728_cov_4.838613"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1146
                     /locus_tag="PKL26_07780"
     CDS             79..1146
                     /locus_tag="PKL26_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS83633.1"
                     /translation="MMIAFDRVSLALGEFALKEISFRVREGDYFFIIGPSGAGKTILL
                     EAIAGLYVPDKGRILLSGRDVSQIPPEKRGVGLVYQDYSLFPHMNVEKNIAFGLKMQG
                     VPAMEMKRTVDNLISRFGIGNLRARSPMTLSGGEMQRVALARALATSPRVLLLDEPLS
                     ALDPSMKEYFIHELREVHQDTGLTIIQVSHDRHEILSLGTRMALIMDGCLEQEGSVRE
                     VYESPVSRRVAEFIGYENVIEGVTISSGDGECLIDAGGMQLRAPFAVPEGRPVTFCIG
                     ADEVVIHPCPVAPVNDENVIPGVVTSLTQAGYLTRAKIDAGITLTAVMIRETAEEMAL
                     KPGSRVSVSIRKKAIRVIVQD"
     gene            1387..1458
                     /locus_tag="PKL26_07785"
     tRNA            1387..1458
                     /locus_tag="PKL26_07785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:gcc)
BASE COUNT          393 a          444 c          503 g          388 t
ORIGIN      
        1 cgatcgcgtt cctgatgata gtcgcaagtt tctcggtgtt cctggtgctg cggattgtga
       61 caagatacct ggggagatat gatgatcgcg tttgatcgcg taagccttgc cctcggcgag
      121 tttgccttaa aggagatctc atttcgtgtc agggaggggg attacttctt catcatcggc
      181 ccgtccgggg cagggaagac gatccttctt gaagcgatcg ccggactcta cgtccctgac
      241 aaggggagga tcctcctctc tgggagagac gtgagtcaga tccctcccga aaaacgcggt
      301 gtcggtctgg tataccagga ttattcgctc tttccgcaca tgaacgtaga aaagaacatc
      361 gcgttcggcc tgaagatgca gggggtcccg gcaatggaga tgaagagaac cgtggacaac
      421 ctcatctcca ggttcggtat cgggaacctc cgtgcacggt caccaatgac tctttcagga
      481 ggagagatgc agcgggtcgc ccttgcgcgt gcactggcca ccagcccgcg tgtccttctc
      541 ctggatgaac ccctttcggc tctcgaccct tcaatgaaag aatacttcat ccatgaactc
      601 agggaggttc accaggatac tggactcacc atcatccagg tgagccacga caggcacgag
      661 atcctctcgc ttgggacccg gatggcactt atcatggacg gatgcctcga acaggaggga
      721 tcggtgcggg aggtctacga atctcctgtt tctcggagag ttgcagagtt catcgggtat
      781 gagaatgtga ttgaaggagt gacaatctcg tccggggacg gggaatgcct gatcgatgcc
      841 ggaggcatgc agttgcgtgc accttttgcc gtgccggaag gaaggcctgt caccttctgc
      901 atcggggccg acgaagtggt aatccatccg tgcccggtgg cgcctgtcaa tgacgaaaac
      961 gtcatccccg gagtggtgac ctcgctcacc caggccgggt acctcacccg ggcaaagatc
     1021 gatgcaggga ttacgcttac cgcggtgatg atacgggaaa ccgcagaaga aatggccttg
     1081 aaaccgggtt cccgcgtcag tgtctcgatc aggaagaaag ccatccgggt gatcgtgcag
     1141 gattgacgtg agtctccttc ataatcaggg gaacggacgc atatccactt tgccggaaat
     1201 gtcatctgca tgccagggca tgcaaacaaa ttcaacccgg ccgggggcag gctattccat
     1261 ttcaatacgt gaaaattggg accggggtcc ggccattctt acaggtggag aggcagcata
     1321 tgcccgtttt gccaggaatt cgatatcctt attagcgatt acctcatatt gtgtagagca
     1381 aaatttgcgc cgatagtgta gtggttatca ctaggcgttg ccaacgccta aacccgggtt
     1441 cgagtcccgg tcggcgcatc atcttctctg aaatttcact cgtgaatcgc tcctttctcc
     1501 ctgttgtctc gtcatgatcg gtatgaggga gaggcacaaa ctgaattccg gctttcgatt
     1561 ttatgcacag cctgaacgcg actgcctgga aggtggaagg gagatcccga acgcaccttc
     1621 aaatatggtc ggaacacaaa ggaacagaga cggatactgt gggaccgatt ggaaatatct
     1681 catcgaggag gagggtgtgc cgatcgaatg gcacctcgcg gaatgact
//
LOCUS       DAOQVJ010000398         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_92156_length_1725_cov_3.360479, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000398 DAOQVJ010000000
VERSION     DAOQVJ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92156_length_1725_cov_3.360479"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..602)
                     /locus_tag="PKL26_07790"
     CDS             complement(30..602)
                     /locus_tag="PKL26_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83634.1"
                     /translation="MAAVSFDTFILLVLLVLVGIVLFLSLVTAIHVIRLSREIAFRQK
                     REEKATGRNAPPAGSDVKTQADTVAAATYTPVAGRKRDFDVTQDAPDIPTGMKNLCAH
                     YDLTSLSISSPDGLLIASSGYPGAINDAAQYSYAFQTGVPIKESGVQVFPVDFGTSPL
                     VGILRSTSALPDTSVDSLQDDIAALLKRWI"
     gene            complement(615..1253)
                     /locus_tag="PKL26_07795"
     CDS             complement(615..1253)
                     /locus_tag="PKL26_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83635.1"
                     /translation="MKKGLELGTILLKLKDENFSGSCSLVCESQAIDMVFQDGRVMLA
                     SCDTFCGDAAMDIISQKQDCIVDASLSDLTPAQLRLTLEFNNGCRVRRQRPSTGKTPA
                     PAPDGWTPVPNQETQKVTSQSPPVKNTGKMPAISSSPVPAPVVIPRHQGERERGPAGS
                     PALDGDEYTIVDRDLDALDAMDLDTMSEKIRMNCKTMIEKLHLEHLIDDSGK"
     gene            1450..>1725
                     /locus_tag="PKL26_07800"
     CDS             1450..>1725
                     /locus_tag="PKL26_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS83636.1"
                     /translation="MLKQILGEFLKIDGVTAAVVVGRDGFVIESAASGKMDIDALGAM
                     ASTGMGTSEAMGKELGKGELNQMLVELDHGPILMSPLSADELIAVVAG"
BASE COUNT          398 a          415 c          500 g          412 t
ORIGIN      
        1 taatgaaccg cacggaaaat gaacgtctct caaatccatc tctttaatag cgcggcgata
       61 tcatcctgca aagaatcaac cgacgtatca gggagtgcgc ttgtactgcg aaggattccc
      121 accaggggag aggtgccgaa atccacaggg aacacctgca ctccgctctc ttttatggga
      181 actcctgtct ggaatgcata actgtattgt gccgcatcgt ttatcgcgcc gggatagcct
      241 gaagaagcga tcaaaaggcc gtcgggtgag gagatggaaa gagacgtgag gtcgtagtgt
      301 gcgcacaggt tcttcattcc cgtggggata tccggggcat cctgagtgac atcaaaatct
      361 ctcttgcgtc ctgcaacggg tgtataagtc gcagccgcaa cagtgtctgc ctgggtcttc
      421 acatcactcc ctgcaggggg ggcattcctg cctgtggcct tctcttctct cttctgcctg
      481 aaggcgattt cgcgggagag tctgatgaca tggatagcag tcacaagaga aagaaacagg
      541 actatcccga cgagaacgag caacacaagg agaatgaatg tatcgaaact aactgcggcc
      601 atggctgttc ttcctcattt tccactgtcg tcgatgaggt gttcgaggtg gagtttctca
      661 atcatggttt tgcagttcat ccggatcttc tcgctcatgg tatcaaggtc catggcatcc
      721 agagcgtcca ggtccctgtc aacgattgta tattcatcgc catccaacgc gggactgcca
      781 gccgggcctc tttcccgttc accctggtgg cgggggatta ccacgggtgc cgggacgggc
      841 gatgaggaga ttgcgggcat cttcccggta tttttgaccg ggggagattg cgaagttact
      901 ttctgcgtct cctggttcgg gacgggcgtc cacccatcgg gcgcaggggc aggggtctta
      961 cccgtggagg gcctctggcg gcggaccctg cacccattat tgaactcgag cgtgagtctc
     1021 agctgggcag gggtaaggtc agagagcgac gcatccacga tacagtcctg cttttggctg
     1081 atgatatcca tagccgcatc accgcagaag gtgtcacagc ttgccagcat aacgcgaccg
     1141 tcctggaaga ccatgtcaat ggcctggctc tcgcagacaa gtgaacagga accggaaaaa
     1201 ttctcatcct tgagtttcag caggatagtg cccaattcca gccctttttt catggaatgg
     1261 aaagtccccc ggggtagttg catcagtagt gatcaatact cttcgattca taaggttatt
     1321 ggtgccggga ttgccgggaa caggaagtga aaacaggcga agaatcccct cgtgcgatat
     1381 gcgaacgagc ttagcaaaca tttttatttt tatatatcaa caaagatgct ttataagtag
     1441 gtggcatgta tgctgaaaca gattttggga gaattcctga agattgatgg ggtcactgcc
     1501 gcagtcgtgg tggggcgtga tggttttgtg attgagagtg cagcttcggg gaaaatggat
     1561 attgatgcac tcggagcgat ggcatctacg gggatgggga catctgaggc tatgggaaag
     1621 gagctgggaa aaggagagct caaccagatg cttgtcgagc ttgatcatgg acctatcctc
     1681 atgtcccccc tctctgcgga tgaattgatc gcggtggtgg ccgga
//
LOCUS       DAOQVJ010000399         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_93061_length_1715_cov_3.556627, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000399 DAOQVJ010000000
VERSION     DAOQVJ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93061_length_1715_cov_3.556627"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..847)
                     /locus_tag="PKL26_07805"
     CDS             complement(257..847)
                     /locus_tag="PKL26_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNS83637.1"
                     /translation="MSTSMLEKIRGFMFNPSQAFRRVKDEEAAETVKYLMILAVFYAI
                     MSALLTAIEIFTHPFLMGLPSMGPNPVEPILFVSWIIVILVVTLVVAVIFGFWLHIWV
                     YLLGGRKGVWQTEKSVFYNLTPTLLLGWIPVIGPLVGGVWSVIIGVIGVRDLHNMPDT
                     KAAFAVVLAIVIASVLAVLVLGAMLLAVISSIPMNV"
     gene            1028..1489
                     /locus_tag="PKL26_07810"
     CDS             1028..1489
                     /locus_tag="PKL26_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNS83638.1"
                     /translation="MVELADLSFGWILVVLGALFLVIETSSPGFFIAVPGTVMIILGV
                     LILLGVDIFSGPTGLVVGVVATVAVSMITIWLYRRINPGETAPTTISRDSLVGMEGRV
                     VRKVIPDSLSGKVLIAGQEWSARSGSGEIPPGVTVRVKASEGVHIVVEEVK"
     gene            1494..>1715
                     /locus_tag="PKL26_07815"
     CDS             1494..>1715
                     /locus_tag="PKL26_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNS83639.1"
                     /translation="MAVIDTLVTIFLVIAIVIIFARGVVIVQPYEQGLAIRLGRYKGR
                     MNPGFRWVFPLITNVIKMDLRTQVMDVPSQ"
BASE COUNT          359 a          477 c          504 g          375 t
ORIGIN      
        1 gccggcataa aggtcggagg tttctttttt ttatgggggg gatgggattg cggatcctcc
       61 ctttaacgca atagaacgcg gaatgagtgt atagaaggta catttttcat ttctccccat
      121 gaaaggtata tccggacgtg tggtcaacga atgaaaaaat cctcctgggg cattatccag
      181 cgtggtgaac cctcgcagga gcgcctcatg gcactcctgc tccccgcagc agtggctata
      241 ccgccactgc tgctccttaa acattcatcg ggattgacga gataaccgcg agaagcattg
      301 ccccgagaac gagcacagcc agcacggatg cgatgacgat tgcgagcacc accgcgaacg
      361 cggccttcgt atctggcatg ttatgcagat ccctgacccc gatgacgccg atgatgaccg
      421 accacacccc gcctaccagc gggccgatca cggggatcca cccaaggagg agagtcgggg
      481 tcaggttgta aaagacgctc ttctcagtct gccagacacc ttttctcccg ccgagaaggt
      541 acacccatat gtgcagccag aacccgaaga taactgcaac gacaagtgtg acgacaagga
      601 tcacgataat ccacgagaca aacaggatcg gctcgaccgg gtttgggccc atcgagggca
      661 accccatgag gaaagggtgg gtgaagatct cgattgccgt cagcagcgca ctcatgatgg
      721 catagaagac tgccaggatc atcaggtact tgacggtctc tgccgcctcc tcgtctttta
      781 ccctccggaa cgcctgtgaa gggttgaaca tgaatccccg tatcttctcc agcatggacg
      841 tgctcatagt aaccaactca tttgcgtggc ggaattttta atttttgcgt atttctcctg
      901 ggttcttttt cccgggagag caggaagtac ggaggggacg aaagtgtcat gcctgccttt
      961 gcccggcctt cgcaccaatt cctcatcttc gagagggttt ttctccccgg ccgtcgatac
     1021 cctgggtatg gtcgagctgg ctgacctctc ctttgggtgg atcctggtgg tgctcggagc
     1081 actcttcctg gtgatagaga cttcgagccc gggattcttc atcgcggtgc cggggacagt
     1141 gatgatcatc ctcggggtgc tcatcctcct gggagtggat atcttcagcg ggcccacggg
     1201 gctcgtggtc ggcgtggtgg caacggttgc ggtctcgatg atcaccatct ggctctaccg
     1261 ccggatcaac cccggcgaga cggcccccac caccatcagc cgcgattcgc tggtgggcat
     1321 ggagggcagg gtcgtccgga aggtcatccc ggattcgctc tccggtaaag tcctcatcgc
     1381 cggccaggaa tggagcgcca ggagcggttc aggggagatt ccgcccggcg ttacggtaag
     1441 ggtaaaggcc tcggaagggg tccacatcgt tgtcgaagag gtgaaatgag cctatggctg
     1501 tcattgatac actggtaacc atctttcttg tcattgcaat cgtgatcatc tttgcacgcg
     1561 gggtggtgat cgtccagccc tacgagcagg gcctggcaat ccgcctcggg aggtacaagg
     1621 ggaggatgaa cccggggttc cgctgggtct ttcccctgat cacgaacgtg atcaagatgg
     1681 acctgcggac gcaggtgatg gacgtcccct cgcag
//
LOCUS       DAOQVJ010000400         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_93587_length_1709_cov_4.322854, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000400 DAOQVJ010000000
VERSION     DAOQVJ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93587_length_1709_cov_4.322854"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1096)
                     /locus_tag="PKL26_07820"
     CDS             complement(224..1096)
                     /locus_tag="PKL26_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNS83640.1"
                     /translation="MAADMIPRIRFGKPDIAVMKREGYAAVDMHVHSNHSDAVPSVRK
                     ILNYARRRGIGVAITDHNEISGVREINGYRPDALVIPGIELDCAEGPHILLYFYSCND
                     LADFFNRHIRERRVGARYTKSFLATDEILTLAEGYPCLKVAAHPFGYFFLDRGILKCD
                     ERGLLPGIVERLDGIEVICGGMTMRVNRMAIDYANSRHIPVTGGSDAHIQSEVGRVVT
                     GVRAESVGDFLDGIRRCESVVAGSSSGIASRMVTGSVIGYHYLPYSVKVLHSGMGSHA
                     GRIMHSMRRIFRPR"
     gene            complement(1166..1354)
                     /locus_tag="PKL26_07825"
     CDS             complement(1166..1354)
                     /locus_tag="PKL26_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83641.1"
                     /translation="MNTSHMMILIFAVFLLVPLGFFFLVISLGNFMYGDSIAGLVFLV
                     VFMACSGAVYFLLKKYRE"
     gene            complement(1443..>1709)
                     /locus_tag="PKL26_07830"
     CDS             complement(1443..>1709)
                     /locus_tag="PKL26_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS83642.1"
                     /translation="ILPLFAGFSASPVFGKAPLTVTFTDESFGNPTFYIYDFGDGFKS
                     MDPNPAHTYRFPGRYTVSQKVLRVGHGTMQSDVEVKQDLILVSA"
BASE COUNT          413 a          483 c          426 g          387 t
ORIGIN      
        1 gtggaaggct tgattcactc ccctggagca gacgtatact gatgtccatc ggccgcctct
       61 tctcgccgat gctctgaaca ggatattgag acacttgcaa catgcaaaaa cacgtcttcg
      121 atagggaagc aattgcccgg aacctcctgg atatctacga cgacttgaaa gaaaaggtca
      181 gtcccgcgac atcttcctgg acggaacggg agatacgatg gccttacctc gggcggaaga
      241 tgcgcctcat ggagtgcatg atccggcctg catgcgaacc cattccggag tggagaactt
      301 tcaccgaata cggcaggtaa tgatagccga tgacactccc tgtcaccatc cttgaagcga
      361 tccccgagga tgaccctgcg accacgctct cgcatctccg tatcccgtca agaaaatccc
      421 cgacagattc ggcgcgtact cccgtgacca cccttccgac ctcactctgg atatgggcat
      481 cggaccctcc tgtaacagga atatgccggg aattcgcata atcaatcgcc atcctgttca
      541 ccctcatcgt cattcccccg cagattacct cgatcccgtc aagacgttcg acgattcccg
      601 gaaggagtcc cctttcatcg cacttcagta tcccgcggtc caggaagaaa tacccaaacg
      661 ggtgggctgc aaccttcagg catggatatc cctccgcaag ggtgaggatc tcgtctgtgg
      721 ccaggaacga cttcgtgtac ctggcaccga cacgcctctc cctgatgtgg cggttgaaga
      781 aatctgcaag gtcgttgcag gaataaaagt agaggaggat gtgaggtcct tcggcacagt
      841 caagttcaat gccgggaata accagggcat ctggccggta tccgttgatc tcccgtactc
      901 cactgatctc gttgtggtcc gtgatcgcaa ctcctattcc cctcctacgg gcataattga
      961 gaatttttcg aacggatggc acagcatctg agtggttgct gtgcacatgc atgtccaccg
     1021 ccgcgtaccc ttctctcttc atcactgcaa tatccggttt cccgaaccgg attctgggga
     1081 tcatgtcagc ggccatgcac ttttccgatt catatgaagg gtatattttt tcacaatcaa
     1141 gactggtttt ttggtatgct cgaggttact cacggtattt tttcaggagg aagtacacag
     1201 ccccggaaca tgccatgaat accaccagga agacgagtcc cgcgatcgaa tccccgtaca
     1261 tgaaattccc cagggagata accaggaaga aaaatccgag cggtacaagg agaaagactg
     1321 cgaagatcag tatcatcata tgggacgtgt tcatgtcagg cacggattcc tcctttatgt
     1381 cgtaattgtc atctcacctg catgtgcgcg gaaactccgg gtaaaaagga aaagcgcata
     1441 tctcatgcgc tcacgaggat caggtcctgt ttcacttcga catcactctg catcgtaccg
     1501 tgcccgaccc tcaacacctt ctggctcacc gtgtagcgac cagggaaccg gtaggtatgg
     1561 gccgggttcg ggtccatcga cttgaaccca tctccaaagt cgtagatgta gaatgtcggg
     1621 ttcccaaatg actcgtccgt gaacgtgact gtaagcgggg ctttcccaaa gacaggcgat
     1681 gcggagaacc ctgcaaataa cggcaggat
//
LOCUS       DAOQVJ010000401         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_93670_length_1708_cov_3.987296, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000401 DAOQVJ010000000
VERSION     DAOQVJ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93670_length_1708_cov_3.987296"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /gene="mutL"
                     /locus_tag="PKL26_07835"
     CDS             complement(<1..1250)
                     /gene="mutL"
                     /locus_tag="PKL26_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNS83643.1"
                     /translation="MAIGREPGEIQVLDQQTVSRIAAGEVVERPASVIKELVENALDA
                     GATRVEVEITSRKGIITGIRVTDNGSGMTPKDADLACVRHATSKIRTLSDLLLVQSLG
                     FRGEALASIAAVSRLSLITRKAGTALSGVRIVNEGGEVIERGECGCPEGTTVEVEEIF
                     FNTPARRKFLRSLPAEMAYITGILERIILSRPDVSFRVLHNRKVLMSGPGGSVHDAAL
                     HLFGTGVARALIPLDFEGSFIRVEGVISHPSLSRQNPYQVFLSINRRPVQSRALSLAV
                     KAGYGTLLPGDRFPVAILDITIDPHLVDVNVHPTKREVRLSREREVKGEISRAVETAL
                     KDMDLTFEARESGKASTQATISPASGSPAYHIDEPSLATVAEPGRSIRADTDRRLRQT
                     ELGLSAGTAGQSPVPDLEVLGTLD"
     gene            complement(1254..>1708)
                     /locus_tag="PKL26_07840"
     CDS             complement(1254..>1708)
                     /locus_tag="PKL26_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNS83644.1"
                     /translation="LHGKKSQGPRTLFATHFHELVTVEGEFSRVKNFHFAVKETGREV
                     VFLRKIIPGATDKSYGIHVAALAGVPRRVTDRARVLLEEMADAESRSSGPRVKRYTQM
                     LLPEIPAADAPAHPVLEMLKNMNLDDMTPLSALSILYDLQKRAREGGR"
BASE COUNT          304 a          533 c          525 g          346 t
ORIGIN      
        1 ttgtcaagag tcccgaggac ttcgagatcc gggacaggtg attgcccggc tgtccctgcg
       61 gagagtccaa gctcggtctg ccggaggcgt cgatcggtat cggcacggat ggacctcccc
      121 ggttctgcaa ccgtggccag ggaaggttca tcgatgtgat aggcgggtga accactggcg
      181 ggggagatcg ttgcctgggt cgaggccttt cccgattcgc gggcctcgaa cgtgagatcc
      241 atgtctttaa gcgccgtttc cacggcccgg gatatctcac ccttcacttc cctctccctg
      301 ctcaagcgga cctctctctt ggtgggatgg acgttcacgt cgacaaggtg cgggtcgatc
      361 gtgatgtcaa ggatggccac cgggaacctg tcgccgggga gaagggtgcc gtatcccgcc
      421 ttcaccgcga gagagagcgc ccttgactgg accggtcgcc tgttgatgct gagaaagacc
      481 tggtaggggt tctgccttga gagcgaggga tgcgagatca ccccctccac cctgatgaac
      541 gacccctcga agtccagcgg gatcagggcg cgtgccaccc cggtgccaaa aaggtgaagc
      601 gccgcgtcgt ggacagatcc accgggcccg gacatcagca ccttccggtt atggaggacc
      661 cgaaacgaga catccgggcg ggagaggatg atccgctcca aaatccccgt gatataggcc
      721 atctctgccg ggagcgagcg caggaatttt ctccgtgcag gggtgttgaa gaagatctcc
      781 tccacctcga cagtcgtccc ctcggggcat ccgcattcac cccgctcaat cacctctccc
      841 ccttcgttta caatccggac acctgagagc gcggtgcccg ccttccttgt gatgagggaa
      901 agcctcgaga ctgcagcgat gcttgcgagc gcctcgccgc ggaacccgag cgactggacg
      961 agcaggagat cggaaagagt ccggatcttg ctcgtggcat gccggacaca ggcgagatct
     1021 gcgtcttttg gcgtcattcc cgaaccattg tcagttaccc tgatcccggt gatgatcccc
     1081 ttgcgcgagg tgatctccac ctcgacgcgt gttgctcccg catcaagcgc gttctccacg
     1141 agctccttta tcaccgaggc aggtcgctcg accacttctc cggccgcgat ccgggacacg
     1201 gtctgctggt cgagtacctg gatctctcct ggttctctcc cgatcgccat ccttcaccgc
     1261 cccccttccc gcgccctctt ctggaggtca tagagaatgg agagggccga gagcggagtc
     1321 atatcatcga ggttcatgtt cttcagcatc tcgaggacag ggtgggcagg cgcgtccgcc
     1381 gccgggattt cggggagaag catctgggtg taccgcttca ctctcggacc tgatgaacgg
     1441 ctttcggcat cggccatctc ttcgaggagc acccgcgccc ggtcggtcac cctgcgtgga
     1501 accccggcaa gcgccgcgac gtggataccg tagctcttgt cggtcgcgcc ggggatgatc
     1561 ttccggagga agacgacctc cctgccggtt tcttttaccg cgaagtggaa gttcttcacg
     1621 cgggagaact ccccctcgac ggtgacgagt tcatggaaat gcgtggcaaa gagggtcctt
     1681 ggcccctggg actttttgcc gtgcagga
//
LOCUS       DAOQVJ010000402         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_94258_length_1702_cov_3.587128, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000402 DAOQVJ010000000
VERSION     DAOQVJ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94258_length_1702_cov_3.587128"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKL26_07845"
     CDS             complement(<1..344)
                     /locus_tag="PKL26_07845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS83645.1"
                     /translation="MPPTATVRELSPAEFPLAEQVWTHYRNQKADPKTERIFGAFVDG
                     KLAATARCTRHKDGLEMDCVYTLDEYRGQGLARKVVDALLAACGKETIYIHSTLVLRQ
                     FYGSLGWRPIGE"
     gene            complement(433..1701)
                     /locus_tag="PKL26_07850"
     CDS             complement(433..1701)
                     /locus_tag="PKL26_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS83646.1"
                     /translation="MFTVNRLEEVLIDQKEAFLRRDPGVPREVDLDRYLGSSHIIVVS
                     GIRRCGKSTLLRQFAEKTGDFLYVNLDDERLFGFEIGDFENLVLVFEKLHPGVRVMLI
                     DEVQNVEHWERFVRRVHDEGYKVILTGSNAHLLSAELGTRLTGRYLQIELFPFSFREY
                     LRCKGVEMGNLTTQKKAEILNHFDYYLKNGGFPEFHTIGDEEVLRRTYDDILFRDIVA
                     RYGIRDVKMFRQMARYLFSNFTKEASYHSLKNALGIKSAMSVRSYARYMEEAYLLFEM
                     FNYQHSLKKQYAGTRKFFVVDNGMRNAVSFRFSGDWGRMLENLVFIELKRRGLSVYFW
                     REDVECDFLVEKKGRIMEAIQCSCTIGEKNREREISGLLAAMDRWKIDSGMMITYTHE
                     EELDLPGGRKVRLIPAWKWLTGNMPPSIQP"
BASE COUNT          361 a          512 c          432 g          397 t
ORIGIN      
        1 ttttcgccaa tcggcctcca cccgagcgac ccgtagaact gccgcagtac gagcgtcgaa
       61 tggatgtaga tcgtctcctt tccgcacgcg gcaagcaggg catcgacgac cttgcgggca
      121 agcccctggc ccctgtactc gtcgagtgta tagacgcagt ccatctccag gccatctttg
      181 tggcgggtgc agcgggcggt cgcggcaagt tttccatcga caaaggcccc gaagatgcgt
      241 tcggtcttcg ggtctgcctt ctggttccgg tagtgggtcc agacctgctc ggcgaggggg
      301 aactcggcgg gggaaagttc gcggacggtg gcggtggggg gcatacaaga atcgttctcc
      361 ttgttggagg ttttggattg ggcgcgggaa aaagataggg gtgggggaaa tgaaattcaa
      421 aaggggcggt tttcatggtt gtatggatgg gggcatgttc cctgtcagcc acttccatgc
      481 agggataagg cgcaccttcc tgccgccagg caaatccagc tcctcctcat gggtatacgt
      541 gatcatcatc ccggagtcaa tcttccagcg gtccatggct gccaaaagcc cggagatctc
      601 cctctcccgg ttcttctcgc cgatcgtgca ggaacactgg attgcctcca tgatccttcc
      661 tttcttctca acaagaaagt cacactccac atcctcccgc cagaaataca cggaaagccc
      721 gcgccttttc agctcgatga agacaaggtt ctcaagcatt cgtccccagt cacccgagaa
      781 acgaaacgag acggcgtttc tcatcccgtt gtccaccacg aagaactttc tcgtccctgc
      841 atactgcttc ttcagcgagt gctggtaatt gaacatctcg aatagcaggt atgcctcctc
      901 catgtaccgt gcataactcc tcaccgacat cgccgacttt atcccgagcg cattcttcag
      961 cgagtggtaa ctcgcctcct tcgtgaaatt tgaaaagagg tagcgggcca tctgccggaa
     1021 catcttcacg tccctgatgc cataccgcgc cacgatatcc cggaacagga tatcgtcgta
     1081 tgtccttctc agcacctctt cgtccccaat tgtatggaat tcgggaaatc ctccgttctt
     1141 caggtagtaa tcaaaatggt tcaggatctc tgcttttttc tgcgtcgtca ggttccccat
     1201 ctccacccct ttgcaacgca ggtattcccg gaacgagaac gggaagagtt cgatctgcag
     1261 gtatctccct gtcaggcgtg tgcccagctc agcactcaaa aggtgagcgt tcgacccggt
     1321 aaggatcacc ttgtagccct cgtcatggac cctccgcacg aacctctccc agtgttcgac
     1381 attctggacc tcgtcgataa gcataaccct gacgcccgga tgcagtttct cgaacaccag
     1441 cacgagattc tcgaaatccc cgatctcgaa cccgaagagg cgctcgtcgt caaggtttac
     1501 atacaggaaa tcgcctgtct tctccgcgaa ctgccggagg agcgtcgact ttccacatcg
     1561 cctgatcccc gaaaccacga tgatgtgtga agatccaaga taccggtcaa ggtcaacctc
     1621 tctcggtact cctggatccc tcctcaggaa cgcctctttt tgatcgatca gcacttcttc
     1681 caggcggttt acggtgaaca tg
//
LOCUS       DAOQVJ010000403         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_94945_length_1695_cov_2.851829, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000403 DAOQVJ010000000
VERSION     DAOQVJ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94945_length_1695_cov_2.851829"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..269
                     /locus_tag="PKL26_07855"
     CDS             78..269
                     /locus_tag="PKL26_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNS83647.1"
                     /translation="MSFDEFEAVRLKDLEGLDQSACAGMMDISQPTFHRLVVSARRKI
                     ADALTHGKILKIEGGHYRF"
     gene            complement(441..824)
                     /locus_tag="PKL26_07860"
     CDS             complement(441..824)
                     /locus_tag="PKL26_07860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNS83648.1"
                     /translation="MAHSSCPFCSPGESDILYRNAHWYARWDAFPATRGHLLVVPFRH
                     VTGYFDTTAEEQHALPEMLIECRKILERQFSPDGYHIVINVGEAAEQKVMHCHVHMIP
                     RYLGKPNDPPGGIRQFILGDHGGEV"
     gene            1403..1624
                     /locus_tag="PKL26_07865"
     CDS             1403..1624
                     /locus_tag="PKL26_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83649.1"
                     /translation="MLLGIGISLYVLLFLLVTYPATCANFERLSGGLYTCGLGPGAYL
                     LAFGIGLALFVWLYLLYSALMSSLEYSWE"
BASE COUNT          368 a          459 c          430 g          438 t
ORIGIN      
        1 ccggcggttg agcagtccgc tgagcgggtc ggtttgggcc tgcaactgca ggtccggcct
       61 atcctgaaga ggttctcctg tcgttcgacg aattcgaggc ggtgaggctc aaggacctcg
      121 aaggcctcga ccagagtgcc tgtgccggga tgatggatat ctcgcaaccg actttccacc
      181 gcctggtggt atcggcacga aggaagatcg ccgacgctct tacccacgga aaaatcctca
      241 aaatagaggg cggacactac cggttctgac cggacgagtc gccggaattt tcaaaacgac
      301 gtgtatccat tcccggaacg cacgcgacac cgcacgaaaa tagtagagac gttttcgcag
      361 ggaacaagag gtttcttctc attctcaatg gtctggcaac gttccacgaa ggagcggcaa
      421 gcccatggtg ttgaagtcct ttacacttcg cccccatgat cgccgagaat aaactgacgg
      481 atacctccag gtgggtcatt gggtttaccg aggtaccgcg gaatcatgtg cacatggcaa
      541 tgcatcacct tctgttctgc cgcttccccg acgttgatga cgatatgata tccatcaggt
      601 gagaattgtc tctccaggat ttttctgcat tcgataagca tctccggcaa ggcatgctgc
      661 tcttcggcag ttgtatcaaa atacccggta acatggcgga atggaacgac caagaggtgt
      721 cccctcgttg ccggaaacgc atcccaccgt gcataccagt gggcattgcg atacaggata
      781 tcggactcgc caggcgaaca gaagggacag gatgaatgtg ccatctcatc tccctcacaa
      841 tcggttcgat ccggttttac tgttatttgt caggcgaatg caccgcaatt ctgctggcat
      901 ggagtatgat gagcagcctt taacgataaa ttccccccat tttccccctt tgatggaaga
      961 tataccttct ctcttcccgt ttgcccccgc ataaaggggg atcgggcatc caacccggca
     1021 tccagatcta atcgggttat gagtgttccc accacctttc agcagatgta aaagcaattc
     1081 tcccgcatgt catgaacgag acccccacca tgtgttatcc tcgtcacggc ggcacatcat
     1141 ttgcttattc aacgaaacga tcattcatag ttcagggttc ccgattcaag agaattgtgc
     1201 gaaggaggct tcccggctga tgggtgcgca tgtgcatcgc ccaggtgaca tacattcgtc
     1261 ttaccgggtt ttcactcgca cacgagttcc gaatggaacc ttctcttgat tattttacag
     1321 attcctttat tttcccctgc accaacataa cgatgacatt atgagaatcg ggaccttttt
     1381 tccaatgcga tatctgccga tcgtgctcct cgggattggg atctcgctgt atgtcctgct
     1441 gtttcttctc gtcacctatc ctgcgacctg tgcaaacttc gagcgcttgt ccggcgggct
     1501 gtatacctgc ggtcttggac ctggggccta cctccttgca ttcgggatcg ggctggcact
     1561 gtttgtctgg ttgtatcttt tgtattctgc gttgatgagt tctcttgagt attcgtggga
     1621 atgatttggg ggggagtgtt cccgaacctt caccctgcat ttcccgggct atgaagccgg
     1681 cacgaatcaa tgatc
//
LOCUS       DAOQVJ010000404         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_95182_length_1692_cov_3.575443, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000404 DAOQVJ010000000
VERSION     DAOQVJ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95182_length_1692_cov_3.575443"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKL26_07870"
     CDS             <1..816
                     /locus_tag="PKL26_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNS83650.1"
                     /translation="IDQDVLGNYLEIWSARATLEIPRRQTGGAVTALILTALEQGLID
                     AVVTVTEDRWTKKPASVVITEAGEIVSHAGSRYNWWVPLVAALKTAVMEKKCRKIAII
                     GVPCVVQAVNRIRTSENDLLKPFGRSIRLVIGLFCTETFDYHMLVEVLLKKEHHVDTW
                     NIDRLDVRGKLEVTMRDGSLVTIPLEELQGAIRPGCRHCTDFTALFSDISAGAVGSRR
                     GHTTLIVRNETGRAFLQEAIAQNALLVGENPVDTSAIERLGRMKAKNVSIDRS"
     gene            955..1620
                     /locus_tag="PKL26_07875"
     CDS             955..1620
                     /locus_tag="PKL26_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83651.1"
                     /translation="MTLLLFGCLLLAIAGIGIAGAADDPGDAPAFPGQPQIPHSFYGA
                     IEAAGNPVPDRVPVEARAEGVSIGIPGNPLYSHAGGYGSADPFTPRLEVQGALSRGTG
                     LTFFVGGIQAEVQAGDSQGPWTVSYPFSPGEVTELNLRVAVEVTPDPAYKETSAQTAP
                     VTTAERTSAGGVNPSTEMMIGLIAILVILAIVAFYFGKRAEKSKREGDQESSGEGDDP
                     SQK"
BASE COUNT          411 a          455 c          490 g          336 t
ORIGIN      
        1 attgatcagg atgtccttgg gaattacctt gagatatggt ctgcaagggc aaccctggag
       61 ataccccgaa gacagacagg gggagcggtg accgcactca ttctcactgc ccttgagcag
      121 gggttgatcg atgcggtcgt cactgtcacc gaagaccgct ggacgaaaaa gcctgcatcg
      181 gttgttatca cggaggcagg agagatcgtc agtcatgccg ggagtcgtta caactggtgg
      241 gttccgctcg tcgcagcact caagaccgcg gtcatggaaa agaagtgccg gaagatcgca
      301 ataatagggg tgccatgcgt cgtgcaggcc gtcaaccgga tacggacgag cgaaaacgat
      361 ctcctgaaac cattcgggag aagtatacgc cttgtcatcg ggcttttctg tacggagacc
      421 ttcgactacc acatgcttgt ggaagtcctg ctcaaaaaag agcaccacgt cgatacctgg
      481 aacatcgatc gcctcgacgt gcgcggaaaa cttgaggtga ccatgcgcga cgggtccctg
      541 gtcacaattc ccctggagga actgcagggg gcaatccgcc cgggatgccg tcactgcaca
      601 gattttactg cgctcttctc ggacatctcg gctggtgcgg tcgggagcag gagaggccat
      661 accacgctca tcgtccggaa cgagaccggg agagccttcc tccaggaggc aattgcacag
      721 aatgccctgc tggtcggtga gaacccggtg gatacaagcg caatcgagag actcggccgg
      781 atgaaagcaa agaatgtgtc cattgaccga tcctgagtgt gatcatccat aatccacgat
      841 catttattgt cagaggttta ttccgtgcat attcatgcgc gtgaacggtc aggaaaagtt
      901 aaatgggatt tcagaggaga ttatgtgaaa aggcagggat atgaactcat gagaatgaca
      961 ctacttcttt tcggatgcct cctccttgca attgcaggga tcggaatcgc aggtgcagct
     1021 gatgatccgg gggatgcccc ggccttcccg ggccagccgc aaatccctca ttcgttctat
     1081 ggagccatcg aggcggcagg gaacccggtt ccagaccgtg tcccggttga agcacgggcc
     1141 gagggcgttt ccatagggat tccaggaaac cccctctact cacatgccgg aggatacggc
     1201 tccgccgatc ccttcacacc ccggctggaa gtccagggtg ctctctcccg ggggaccggc
     1261 ctgaccttct tcgtcggcgg gatacaggca gaggtgcagg ccggggattc acaagggccc
     1321 tggaccgtca gctatccatt ctctccaggt gaagtgactg aactcaacct cagggttgca
     1381 gtcgaagtga cccctgaccc cgcatacaag gagacttcgg cgcagactgc ccctgtcaca
     1441 actgcagaaa gaacctctgc cggaggagtg aacccctcga cggagatgat gatcggcctc
     1501 attgcgatac tggtaatact cgcgatcgtt gcattctatt ttgggaaaag agcggaaaaa
     1561 tcaaagagag aaggggacca ggagagcagc ggagaggggg atgaccccag ccagaaatga
     1621 tactgctcag accgcctgaa ccatccccat cctatcattt tatcatgata tcaggatacg
     1681 acatacccag ag
//
LOCUS       DAOQVJ010000405         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_96059_length_1683_cov_3.979115, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000405 DAOQVJ010000000
VERSION     DAOQVJ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96059_length_1683_cov_3.979115"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="rplV"
                     /locus_tag="PKL26_07880"
     CDS             complement(<1..264)
                     /gene="rplV"
                     /locus_tag="PKL26_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNS83652.1"
                     /translation="MDMARTLYSQDVDGDNVARGKANELPISPKHAMEIASFIKFKRT
                     GEAISYLEQVIDQKKAIPFRRFNRKVAHKRGLEGWDAGRYPKKA"
     gene            complement(264..677)
                     /locus_tag="PKL26_07885"
     CDS             complement(264..677)
                     /locus_tag="PKL26_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNS83653.1"
                     /translation="MAKKTQKRLPRRREEFTYRGFRLDDLRAMGMSELVPLMPSRARR
                     KVNRGLTRGEEHLLTQFRKGDGAIRTHLRDMIITPEMVGRPVEIHNGKEFVKVEIQPE
                     SLFHYLGEFAPTRKKVSHGSAGIGATRSSKYVPLK"
     gene            complement(689..1405)
                     /locus_tag="PKL26_07890"
     CDS             complement(689..1405)
                     /locus_tag="PKL26_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNS83654.1"
                     /translation="MGHRITTQSRGKGGPTYRAPSHRYKAALRHVSSGEITVRGKVED
                     IEHDPARHAPIALVRLENGSKVYMLITEGMGVGDSVAWGAEADVRNGNTLPLGAIPTG
                     TFICNIEAQPNDGGKFVRSGGVQAVVVDKSEGRVGVTMPSGKTKWFNDRCRATVGIVA
                     GGGRGEKPFVKAGKKFHKMQNTASNWPRVRGVAMNVIDHPFGGGGHQHPGRPKTVARG
                     TSPGRAVGHMAARKTGLSKK"
     gene            complement(1415..1663)
                     /locus_tag="PKL26_07895"
     CDS             complement(1415..1663)
                     /locus_tag="PKL26_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNS83655.1"
                     /translation="MVLSYPFVTEKAMMMLENQNKLQFMVDSKATKSQIKKEIEQAFE
                     QEVTHVSTMMTMSGQKKAVISFANEKAAEEILSRLGIM"
BASE COUNT          248 a          567 c          457 g          411 t
ORIGIN      
        1 cgcctttttt ggatagcgtc ctgcatccca tccttcaagg ccgcgcttgt gggccacctt
       61 ccggttgaac ctccggaagg ggatggcctt cttctggtcg atgacctgct cgaggtagga
      121 gatggcttct cccgtgcgct tgaacttgat gaagctggct atctccatgg catgcttcgg
      181 ggagatggga agctcgttcg cctttccccg cgccacgttg tcgccatcga catcctgtga
      241 atagagtgtt cttgccatat ccatcacttc agcggcacgt acttgctcga ccgggtcgca
      301 ccgataccgg cgctcccgtg gctcaccttc ttccttgttg gggcgaactc gcccaggtag
      361 tggaagaggg attcgggctg gatctccact ttgacgaact ccttcccgtt gtggatctcc
      421 accggccttc ccaccatctc cggcgtgatg atcatgtcac gcaggtgagt gcggatcgca
      481 ccgtcgcctt tccggaactg tgtgaggagg tgctcctcgc cccgggtgag gccacggttc
      541 acctttcggc gggcgcggga cggcatcagc ggtaccagtt cggacattcc catcgcacgc
      601 aggtcgtcca gccggaaccc ccggtaggtg aactcctcgc gccgcctcgg aagtctcttc
      661 tgtgtcttct ttgccattgt cttacctctc acttcttaga aagccccgtt ttcctggcag
      721 ccatgtgccc gacagcgcgt cctggcgagg tcccgcgggc gaccgtcttc ggccgccccg
      781 ggtgctggtg tcctccgccc ccgaagggat ggtcgatcac gttcatcgcc actcccctca
      841 cacggggcca gttggacgcg gtgttttgca tcttgtggaa cttcttcccg gccttgacga
      901 agggcttctc gcccctgcct ccgccggcaa cgatcccgac cgttgcccgg cagcggtcgt
      961 tgaaccattt ggtcttgccg ctcggcatgg tcacgccgac ccggccctcg ctcttgtcga
     1021 ccacaactgc ctgtacgccc ccggaacgga cgaacttacc tccgtcgttt ggctgcgcct
     1081 cgatgttgca gataaacgtc cccgtgggga ttgcgccgag cggaagggtg ttcccgttcc
     1141 gcacatcggc ttccgcaccc catgcgacac tgtcgccgac gcccatgccc tcggtgatca
     1201 gcatgtagac cttgctgccg ttctcgagcc tcaccagggc gatcggtgcg tgccttgccg
     1261 ggtcatgctc gatgtcctcg accttcccgc gcaccgtgat ctcgccgctg ctcacgtgcc
     1321 gcaatgcggc cttgtaccgg tgggagggag cccggtaggt cggacctcct ttcccccggc
     1381 tctgtgtggt tatacgatgt cccatattcc ccacctacat gataccaagc cggctgagaa
     1441 tctcctcggc cgctttttca ttggcgaaac tgattaccgc cttcttctgg ccactcatgg
     1501 tcatcatcgt ggacacatgg gtgacttcct gctcgaatgc ctgctcgatc tccttcttga
     1561 tctgactctt cgtggccttg ctgtccacca tgaactggag cttgttctgg ttctcgagca
     1621 tcatcatggc cttttccgtc acgaacggat aactgagcac catattactg ccctcccaat
     1681 ttc
//
LOCUS       DAOQVJ010000406         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_96538_length_1678_cov_3.247689, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000406 DAOQVJ010000000
VERSION     DAOQVJ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96538_length_1678_cov_3.247689"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(433..>1678)
                     /locus_tag="PKL26_07900"
     CDS             complement(433..>1678)
                     /locus_tag="PKL26_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83656.1"
                     /translation="EAPVVQEIRLDDIPALISDHEPVWDAELVQAASVSRKKILSTRD
                     RLLAAVRRLTESKRDAAFHPKLEKIVQNSLPQFEKAIIFALSRPLPEGADEFYQACTE
                     SLKGCVKGLAGPGRYLRNVFPEEMKEIRALIDEFGKEVNLLTPMVAQSRARREKVRVI
                     LEAYSRAREMETALDGLSKEIPTLEKEIGGLRRRRDENQGTIESCDRQMKDDPAFKEA
                     GALAERSMKEMSEIGRELQGTFSTLVHVLRKAEKITHRAETEKLARDMRRVIDLVSSK
                     EVPAAMDVEREVGAVLPIVSSMIASGEVQLKNQEERDLFFSPEKIPWILSKMIREREK
                     AGERVAIAEAQLAAHPIRVLRGKAEDDLRSIRSELKEKERQLQDLKGRQKEMEADYPR
                     LSLQMETHLSALLEGTWHIATG"
BASE COUNT          337 a          510 c          412 g          419 t
ORIGIN      
        1 atgcgagggc tggtgacttc acgtactccc ctttcacccg gaaatacctg gaatgaacgc
       61 agaacctgca atgctcgacc gtctcctcct tcccgggaag gcagggaacc ttccccttgc
      121 gcacctcaag gacccggtat tcgtcttccg gcatcagaat tctcctttcc agtgttcact
      181 tccgatctat atctaccctc cagtcccgga caaacacggg tgcaacgggg cttgacaact
      241 attggattgt aattcaatcg cgcaatgcat gtcataagtc ctcgtcactc atggtggaaa
      301 actttcattg aaccggaaac aactttaaaa agggggcttc accaggcaca aagggttggt
      361 cctggccagc aattctccgg atacataaga ggtttatacc aacgatatgc cctgctgctg
      421 aaactgcagc ggtcatcccg tggcaatatg ccaggtgccc tcgagcaggg cagaaagatg
      481 cgtttccatc tgcagggaaa gcctggggta atcggcctcc atctccttct gtcttccctt
      541 gagatcctgt agttgccgct ccttctcctt gagttcggac cggatcgatc tcaaatcgtc
      601 ctctgccttc ccgcgcagca cccggattgg gtgagctgcc agttgtgctt ctgcgattgc
      661 aaccctctca cctgcctttt ccctctctcg aatcattttc gagagaatcc aggggatctt
      721 ttcaggggaa aagaagaggt ccctctcttc ctggttcttc aactgtactt cccctgatgc
      781 aatcatgctg gacacgatcg gcaggacggc accaacctcc cgttccacat ccatagcagc
      841 ggggacctct tttgacgaga ctaaatcgat cacccttctc atgtcccggg caagcttctc
      901 cgtctctgcc cggtgggtga tcttttcggc ttttcgtagc acgtgaacaa gcgtcgagaa
      961 tgttccctgg agctcccgcc cgatttcgga catctctttc atcgaacgtt ccgccagagc
     1021 gccggcttcc ttgaatgcag gatcatcctt catctggcga tcacaggatt caatggtgcc
     1081 ctggttctcg tcgcgccttc ttctgagccc cccgatctcc ttttcaaggg tcgggatctc
     1141 ctttgaaagc ccgtccagtg cagtctccat ctcgcgggca cgtgaatatg cctcaaggat
     1201 gacgcggacc ttctcgcgcc ttgcccttga ctgcgccacc ataggggtaa ggagattgac
     1261 ctcttttccg aactcgtcga taagtgcgcg gatctctttc atctcttcag gaaagacatt
     1321 tcggagatat cgtcccggcc cggcaagccc tttcacgcac cctttcaggg attccgtgca
     1381 ggcctggtag aactcatctg caccctctgg cagtgggcgg ctcagtgcaa agatgatcgc
     1441 tttctcgaac tgggggaggg aattctgcac gattttttcg agtttcgggt ggaacgcagc
     1501 atccctcttg ctctcggtaa gccgccggac agcggccaga agacggtccc gtgtcgagag
     1561 gatcttcttc ctagagacgg atgctgcctg cacaagctcc gcatcccaca caggttcatg
     1621 atctgagatg agggccggga tatcatccag cctgatttcc tgcacgacag gcgcttcc
//
LOCUS       DAOQVJ010000407         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_96714_length_1676_cov_4.143738, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000407 DAOQVJ010000000
VERSION     DAOQVJ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96714_length_1676_cov_4.143738"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1072
                     /locus_tag="PKL26_07905"
     CDS             179..1072
                     /locus_tag="PKL26_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNS83657.1"
                     /translation="MINERYLKRIQVKPGMKVRLGEYYTGWAQNDALKEAGKEAVKER
                     AMEILEENRAQLASAQELLWASNIYSVLIILQGMDTAGKDGTIKHVMSGVNPQGCKVH
                     SFRVPTDEELDHTFLWRYWRALPNRGEIGIFNRSYYEDVLVVKVHPERLVNLPPGKRG
                     KKFWNARYEDINTFERHLIRNGTVILKFYLHISEDEQKKRLLDRLEDREKLWKFSLSD
                     LEERKFWGTYQEAYEDALTMTSTEEAPWYIIPADHKWVARTLIADIITTRIQSLDLAY
                     PTVSGEAIAQLKDARRQLEEE"
     gene            1090..1494
                     /locus_tag="PKL26_07910"
     CDS             1090..1494
                     /locus_tag="PKL26_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNS83658.1"
                     /translation="MDLQEFLHVHPVKSRLLKLAAGGACEHCGETYPLSLLEMHVIDP
                     RTGAEGDRPDMQKELLILCPECHRFFHARPVQKSVQRELVRYRPKDVKAAMRRILGTR
                     PRTYVPPETDDPEAIFAEMFESGALDLCLNGG"
BASE COUNT          436 a          456 c          456 g          328 t
ORIGIN      
        1 atctgtcaaa aatcgtgaat cacagatatc acatcgtttt tcgcgatcct attgtgaaaa
       61 ttcctcctcc acaacgaccg tcccgatttc ttccaactgt atgccaagtg ataaaggttc
      121 gccctgcaaa gagagtgatc gctgcaccag cgagatgtca cgcaggggat gaacacaggt
      181 gataaacgaa cggtatctca agcgcatcca ggtcaaacca ggaatgaagg tcaggcttgg
      241 cgagtactat accggctggg cccagaacga tgccctgaag gaggcaggaa aggaagcggt
      301 caaagagcgt gcaatggaga tactcgaaga gaaccgggcg cagcttgcat ctgcgcagga
      361 actcctgtgg gcgagcaata tctattcggt ccttatcatc ctccagggga tggacaccgc
      421 ggggaaagac ggcaccatca agcacgtgat gtccggggtg aatccccagg ggtgcaaggt
      481 gcacagcttc cgggtcccca ccgacgagga gctcgaccac acgttcctct ggaggtactg
      541 gagagccctc ccaaaccgcg gggagatagg catcttcaac cgctcctatt acgaggacgt
      601 cctggtggta aaggtacacc cggagcggct ggtcaacctt cctcccggga aacgggggaa
      661 gaagttctgg aacgcccgtt acgaggatat caacacgttc gagcggcacc ttatacggaa
      721 tggcaccgta atcctcaagt tttacctcca tatttctgaa gacgaacaaa agaaacgact
      781 cctggaccga ctcgaagaca gggagaagtt atggaagttc tcactctcag accttgaaga
      841 aaggaagttc tggggtacct atcaggaagc gtatgaggat gcacttacca tgaccagcac
      901 ggaggaagcc ccctggtaca tcatccccgc agaccacaag tgggtcgcac gcaccctgat
      961 tgcagacatc atcaccacgc gaatacaatc ccttgacctt gcgtatccca cggtttcagg
     1021 agaggcgatt gcacagttga aggatgcacg ccgccagctg gaagaagagt agattgaggc
     1081 gaaaaagtca tggatctcca ggaattccta cacgttcatc ccgtcaaatc gaggctgctg
     1141 aaacttgctg caggcggggc gtgcgagcat tgcggagaga cataccccct ttcgctcctc
     1201 gagatgcacg tgatagatcc ccgcaccggt gcggaaggtg atcgtcctga tatgcagaaa
     1261 gaattgctta tactctgccc ggaatgccac cgcttcttcc atgcaagacc ggtccagaaa
     1321 tccgttcagc gggaacttgt cagatatcgc cccaaggatg tgaaagctgc catgaggcgc
     1381 atcctgggta cccggccacg cacgtatgtc ccgccagaga ccgatgatcc tgaagcaatt
     1441 tttgcggaga tgttcgagag cggggcactg gacctctgcc tgaacggtgg ttgagcgcac
     1501 aggactgttg gttgggacga ggagaaataa tctgggacgg gcaatccgga acacggagac
     1561 ccgctcacgg ataaaaaaga atcaaaggat gtttttcagc ctgtagccat cccttgtctc
     1621 ctcggctata cctctctcca ccagttccct ggccgcaaag accacgtcat ccattc
//
LOCUS       DAOQVJ010000408         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_96816_length_1675_cov_3.500617, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000408 DAOQVJ010000000
VERSION     DAOQVJ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96816_length_1675_cov_3.500617"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKL26_07915"
     CDS             <1..1205
                     /locus_tag="PKL26_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS83659.1"
                     /translation="PRRYHTSVALADGSILIIGGECSGYLNDVVHLVKEGTPWSVRKY
                     LLPLDQGRYHHSSAVLPDGSVILSGGQTPGGIKNDVWRFPTANKEKNPRHVYKAAGTY
                     SVTLQVFNEDGSNTTRKGSNITVIPELPAPEPVGIDPGVGQNTGKINIINLSGANFDT
                     INTPSVRLNRTGHPDILASNVTTPSSSRITCTFDLAEQPAGFWDVAVENPDGKTGVLG
                     QGFCITQGPPTASFVGSPISGNTPHKVSFTDTSTGFPTSWRWTFGDGGTSEERSPSHS
                     YTVAGTYTVALTVQNEGGTDALIRTGYITVTAARPPVITSFAPAKMVHGKTVNVAIIG
                     KYFQTGATAKLVWGTVTIPVKIKTLTPPSKIAGSVTIPAPARGKYSLVVTNPDGGTCT
                     RANVITIT"
     gene            1261..>1675
                     /locus_tag="PKL26_07920"
     CDS             1261..>1675
                     /locus_tag="PKL26_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83660.1"
                     /translation="MKFDEIRVSWFIAVIITFAITSPAWADVPVNSTRETTGISSMTS
                     LDCMGAVTQSNSLAWEQSSSSLHNPPLSPGGIYLVWFDIFGNPVFGWTADPLLAELLE
                     EPIPPGEVRYTAGYTATTLATQGTTHLTRMVDVDTS"
BASE COUNT          416 a          449 c          456 g          354 t
ORIGIN      
        1 caccccggcg ataccatacc agcgtcgcat tggctgatgg aagcattctg atcataggtg
       61 gcgagtgctc gggatacctg aacgatgtgg tgcaccttgt aaaagaagga acgccctggt
      121 ccgtccggaa atatctgttg cccctggatc agggacgata ccaccactca agtgccgtgt
      181 tgccggatgg aagtgtcatc ctctcgggcg gacagacgcc aggtggtatc aagaatgatg
      241 tatggcgatt tccaactgcg aacaaagaga agaatccccg acatgtctac aaggctgcag
      301 gcacctattc cgtgacgctc caggtattca atgaagatgg ctccaacacc acaagaaagg
      361 ggtcgaatat cactgtcatt cctgaactgc ctgcaccgga accggttgga attgatcctg
      421 gagtgggtca gaatacggga aaaatcaata tcataaacct ttccggggcg aatttcgata
      481 ccattaacac cccttcggtg aggttgaacc ggacaggcca tcctgatatt ttggcgagta
      541 atgtcaccac gccttcgtca tcccggatca cctgcacgtt tgacctggcg gaacagcccg
      601 ccggcttctg ggacgtggcg gtcgaaaacc ccgatggaaa gacaggggtt cttgggcagg
      661 ggttttgcat cacacaggga cccccgactg catcgttcgt cggttcacca atatccggga
      721 atacaccgca taaggtctct ttcactgaca cgtcgaccgg tttccctacc agctggcgat
      781 ggacattcgg ggatggagga acaagtgagg agagaagtcc atcccattcc tataccgtgg
      841 cgggaaccta tacagtggca ctgaccgtcc agaatgaagg aggaacagac gcgctcataa
      901 gaacagggta catcacggtc actgcggccc ggccccctgt aatcacctcg tttgcccctg
      961 cgaaaatggt ccacgggaag accgtgaacg tagcaattat cggcaaatat ttccagaccg
     1021 gtgcgaccgc gaagctcgtt tgggggactg ttactattcc tgtgaagata aaaaccctca
     1081 cacccccgtc aaaaatcgcg gggtcggtca ctattcccgc accggcaagg gggaagtata
     1141 gtctggtcgt taccaatccc gatggaggaa cgtgcacgag ggcgaatgtc atcacgatta
     1201 cctgaaaggt gtcctaataa tgaacgcgac gattgaaggg aatcaaggat gtgaaggaga
     1261 atgaaattcg atgagattag ggtctcctgg tttatcgcgg tcataattac tttcgcaatc
     1321 accagcccgg catgggcaga cgtcccggtc aactcaacgc gggaaacaac gggcatctcc
     1381 agcatgacaa gcctcgactg catgggcgcg gtcacgcaat cgaactcgct cgcctgggag
     1441 cagtcgagct catcgctcca taatccgcct ctttctcccg gcggtatcta cttggtatgg
     1501 ttcgacatct ttggaaatcc cgtatttggc tggactgctg atccattgct tgcggagttg
     1561 ctggaagagc ctattccacc gggggaggtc cggtataccg caggatacac tgcgactact
     1621 ctggcaacgc agggaaccac tcacctgacc agaatggttg atgtcgatac ctcga
//
LOCUS       DAOQVJ010000409         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_96913_length_1674_cov_4.151946, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000409 DAOQVJ010000000
VERSION     DAOQVJ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96913_length_1674_cov_4.151946"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..710
                     /locus_tag="PKL26_07925"
     CDS             114..710
                     /locus_tag="PKL26_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS83661.1"
                     /translation="MPPREILNTPRLWLRRFSIDDAQSVQEHADDMCIAAGVLEIPHP
                     YTLEDAEQWIGQHQGLWQRGEHYIFAITEKETTDIAGAASLRVNCAHSHAELGYWIGR
                     PFRCRGYATEAAGALLEFGFLGLGLHRIYARHLDWNTPSSRVLVRIGMHFEGAMKGHA
                     CKWGSFHDVHFYGIQADDFYPHYLDGSRNRVVPCKGGQ"
     gene            711..1550
                     /locus_tag="PKL26_07930"
     CDS             711..1550
                     /locus_tag="PKL26_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNS83662.1"
                     /translation="MSPGLPILISIPHGGVGIPEEVAGAVALDPPALSYYSDPASERL
                     FGFKAEVKAFASARVSRVIVDLNRPPYHLPPRRRDGVVKFQTSLGAPVWKEGMQPDIQ
                     AIHRLLLRHYFPYHAKLDSLLDSRKIAVAFDCHSMVPVGLPGQPDEGQPRPLICLGNN
                     GDRNGHPRRGALCTCPPEWIRQLAERFRDEFPGAGSVTLNHPFSGGFISNAHYWHKGI
                     PWIQVEVNRSLYEGGNSSPQQGALLDMQRMERLKEIIRGVLSMWYMEVVKEDSRTPPL
                     FQE"
BASE COUNT          350 a          495 c          500 g          329 t
ORIGIN      
        1 gctcgtgaag aaggcccgcc ctggcgtgtg accccgcgga taattttttc cgggtatcac
       61 aggaactaca gtctcatatg atacccggtt ccaaatagac atgtgaatgt tccatgcccc
      121 cccgagagat cctgaatacg ccgaggttat ggctccgacg tttctcgata gacgatgccc
      181 agtccgtgca ggaacatgca gatgacatgt gcattgcggc aggcgtgctt gagatccccc
      241 atccctacac cctggaggat gcggagcagt ggatagggca gcaccagggc ctctggcagc
      301 gtggggaaca ctacatcttt gccataaccg agaaggagac caccgacatt gccggtgctg
      361 caagcctcag ggtgaactgt gcacattccc atgcagaact cgggtactgg ataggaaggc
      421 cgttccggtg caggggatac gcgactgaag ccgcgggagc gctgcttgag tttggcttcc
      481 tcggcctcgg cctacaccgc atctacgcga ggcacctcga ctggaacact ccctcatccc
      541 gcgtgctcgt gcgcatcggt atgcactttg agggtgcaat gaaagggcac gcatgcaagt
      601 gggggtcgtt tcacgatgtg cacttctatg gcatccaggc cgatgacttt tacccgcact
      661 accttgacgg gagccgcaac cgggtagtac catgcaaggg ggggcagtga atgtcaccag
      721 ggctcccgat tctcatctcg attccccacg ggggagtcgg catcccggag gaggtcgcgg
      781 gagcagtcgc ccttgatccg cctgcactct cctattacag cgaccccgca tcggaacgtc
      841 tttttgggtt caaggcagag gtgaaagcct ttgccagcgc cagggtgtcg cgggttatcg
      901 tcgacctgaa ccgccctccg taccatcttc ctccccggcg ccgtgacggg gtggtcaagt
      961 tccagacctc gcttggggcg ccggtctgga aagaaggcat gcagccagat atccaggcaa
     1021 ttcacaggct cctcctgcgt cactacttcc cttaccatgc aaaacttgac agtctgctgg
     1081 acagccggaa gatcgcagtc gcgttcgact gccacagcat ggtccctgtg ggattgccag
     1141 gccagcctga cgagggacag ccgcggccac tcatatgcct tggtaacaac ggggaccgga
     1201 acggacaccc gagaagaggg gcactctgta cctgcccgcc ggagtggatc cggcagctgg
     1261 cagaaaggtt ccgggacgag tttccgggag cagggagcgt cacgctcaac cacccgttct
     1321 cgggagggtt catctcgaat gcgcactact ggcacaaggg tatcccctgg atccaggtcg
     1381 aggtgaaccg ctcactgtat gagggtggca attcgagccc acagcagggc gcccttcttg
     1441 acatgcagcg catggaacgg ctgaaggaga ttatccgggg tgtcctttcc atgtggtata
     1501 tggaagtagt gaaggaggat tcccgaaccc ctcctctatt ccaggagtag aacgtccctc
     1561 cttctggttg tgcgtaatgc aggcacatcc cctcgcttca tatccctgtg tacgcagccg
     1621 tgacggcacc aaggaacacc aggcagacga agatgcaggt caggagaggc ccgc
//
LOCUS       DAOQVJ010000410         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_97121_length_1672_cov_3.504020, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000410 DAOQVJ010000000
VERSION     DAOQVJ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97121_length_1672_cov_3.504020"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..751
                     /locus_tag="PKL26_07935"
     CDS             122..751
                     /locus_tag="PKL26_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNS83663.1"
                     /translation="MGIKEWLIPQDEVFFTLFERLAAVVVLASYRIVDLVDDYTSVRE
                     KVHQIEQLEHEGDKITHEIYEQLNQTFITPLDPEEISKLASALDDILDYIKSTATRMY
                     HYGIPGTDPHMVELAKLIQLSVVELEEAVKGIRSMKNPRQIEQRCIEVNRLENLADDV
                     LHHAYDDLFRSNDAIAIIKRKDIYEYLEIATDKCEDVANVLSDIAIRHS"
     gene            758..>1672
                     /locus_tag="PKL26_07940"
     CDS             758..>1672
                     /locus_tag="PKL26_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNS83664.1"
                     /translation="MENIVIAGIFLALLFNFVNGLNDAANSIATVVATRVLSPLQAVL
                     MAAFFNMAGPFIFTTVVAKTIGQGIVESGFLTTEVILVGMCAAVLWVFLSSVAGIPIS
                     ASHALVGGLIGSAIAFGGIGSVLWPSMDLFLRAVVMAAVGGICGAVVLSMTARRKGEE
                     NWPEYTALGALCGISIVIPAGVITGILPVSGILAIVIFIVVSPTLGMIAAYLLGMAII
                     RLFRKANSKWMNFIFSKLQVFSAAFYSMGHGSNDAQNAMGVITALLVAAGLLSEFVVP
                     AWVIIISGLAIALGTFLGGWRVVETMGKK"
BASE COUNT          338 a          452 c          485 g          397 t
ORIGIN      
        1 agatcgcttc cttggcatgg aagaggccgc gggttcaatt cccgctcggt ccatctcatt
       61 cttgggatca tcaaaccgga gtgtttttcc ctgctgctct gaaatctcct tgtgatacgc
      121 tttgggcatc aaggaatggt taatcccgca ggatgaggtg ttcttcaccc tctttgagag
      181 gctggccgcg gtggtggtcc ttgcctccta ccgcatcgtg gacctcgtgg acgactacac
      241 ctctgtccgg gagaaggtcc accagatcga gcagctcgag cacgaaggcg acaagatcac
      301 ccacgagatc tacgaacagc tgaaccagac cttcatcact cctcttgacc cggaagagat
      361 ctcaaagctt gcctctgcgc tcgatgacat cctcgattac atcaagagca ccgccacgag
      421 gatgtatcat tacgggattc cgggaacgga tccgcatatg gtggagcttg cgaaactgat
      481 ccagctgtcc gtcgtcgagc tggaagaggc ggtcaaaggg atcaggtcaa tgaagaaccc
      541 ccgccagata gaacagcggt gcatagaggt gaaccggctg gagaaccttg ccgatgacgt
      601 ccttcaccat gcctacgacg acctcttcag gtcgaatgat gcgattgcta tcatcaagcg
      661 caaggacatc tacgagtacc tggagatcgc gaccgacaag tgcgaggatg tcgcaaacgt
      721 actctcggac atcgcgatac ggcattcatg agtgaacatg gagaatatcg tcatcgccgg
      781 gatattcctt gcactcctct tcaattttgt caacggtctc aatgacgcgg caaattccat
      841 cgccaccgtg gtagccacca gggttctttc ccccctccag gccgtgctga tggcggcatt
      901 cttcaacatg gcggggcctt tcatttttac cacggtggtt gcaaagacga ttggacaggg
      961 aatcgtggag tccggttttc tcactacgga ggtcatcctg gtggggatgt gcgcggcggt
     1021 cctgtgggtt tttttatcgt ctgttgcagg cattcccata tcggcaagcc acgcgcttgt
     1081 cggagggctt atcgggtctg caatcgcgtt cgggggtatc gggtcggttc tctggccttc
     1141 aatggatctc ttcttgaggg cggtggtgat ggccgcggtg ggcggcatct gcggggctgt
     1201 tgtcctctcc atgaccgcga gaaggaaggg ggaggagaac tggcccgagt ataccgctct
     1261 tggggcactg tgcggcatat ccatcgtgat tccggcggga gtgattacgg ggatcctccc
     1321 cgtaagcggc atcctggcca tcgtgatctt catagtcgtc tcacccaccc ttgggatgat
     1381 cgccgcttat ctcctcggga tggcgatcat ccggctgttc aggaaagcga actccaagtg
     1441 gatgaatttt atcttctcga aactccaggt cttctctgct gccttttaca gtatggggca
     1501 tgggagcaat gatgcccaga atgcaatggg agtcatcacc gcgctcctgg tggccgcggg
     1561 gctcctctcc gagtttgtcg tcccggcatg ggtaatcatc atttcaggcc ttgccatcgc
     1621 gctcggcaca tttctcgggg gctggagggt agtggaaacg atgggaaaga ag
//
LOCUS       DAOQVJ010000411         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_97755_length_1665_cov_4.211180, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000411 DAOQVJ010000000
VERSION     DAOQVJ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97755_length_1665_cov_4.211180"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKL26_07945"
     CDS             complement(<1..1037)
                     /locus_tag="PKL26_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS83665.1"
                     /translation="MIIQQFFIPGIAHSSYIVAGNRTCAVVDPSRDVGRYISAAREMG
                     LRITHILETHLHADFVSGHLDLAEATGAEIYAPESGKCKFPHNALVEGDEIPLEHIHF
                     RVIETAGHTPEHVSYIATDTSRGDTPVALFCGDTLFVGDVGRPDLFPGKAEALASSLY
                     DNLHTKIMTLPDECEVYPAHGMGSLCGRAMAAKRTSTVGYEKKYNYALRISDRDEFVR
                     ALTSDMPAAPDHFARCSEINRAGPALMRTLAQPGPVDPKTFSEWIRRNDAVLLDARSY
                     PAFSGVHIPGAWHIDLSGNFSTQAGWILPPERDILLVVESRNQAEEAALQLRRVGFDR
                     VPGFLEGGMLA"
     gene            complement(1054..>1665)
                     /locus_tag="PKL26_07950"
     CDS             complement(1054..>1665)
                     /locus_tag="PKL26_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNS83666.1"
                     /translation="LTHPEGDAVRHGMAHLAVLIFASSLALTMTGVPLSLALLTGSAA
                     MVVSGVLTIDEAYRAIEWKTIALIAGLIPLGIAMEKTGAAALVAGALIGSLSGAHPLV
                     IMCAVAVLATVLSLVISNVAATVLLVPLVMIAGAGTGVDPRALALLVAVCASNSFILP
                     THQVNALLMGPGGYSTKDFIKAGSVMTVIFIGVAVTLIYLLIA"
BASE COUNT          367 a          467 c          520 g          311 t
ORIGIN      
        1 catgcaagca ttccgccctc gaggaaaccc ggcacccggt caaatccgac gcggcggagc
       61 tggagcgccg cttcttccgc ctggttcctg gactcgacga ccaggagaat gtcccgctct
      121 ggcggaagaa tccatcccgc ctgcgtggaa aagtttccgg acaggtcgat atgccaggcg
      181 ccggggatat gcactcccga gaatgccggg taactccggg catcgaggag gaccgcgtca
      241 ttcctgcgga tccattcgga gaaggttttc gggtcgacag gcccaggttg cgcgagtgtc
      301 cgcatcaggg ccgggcctgc ccggttgatc tcagaacatc gtgcgaagtg atcgggggca
      361 gcaggcatgt ccgaagtaag ggcccggaca aactcatccc tgtcacttat ccggagtgcg
      421 taattgtact tcttctcgta gcccacggta cttgtcctct tggctgccat cgcccttccg
      481 cataaggacc ccatcccgtg ggcgggatag acctcgcact catcggggag ggtcatgatc
      541 ttggtatgga ggttgtcgta gagcgaggac gcaagcgctt ccgccttccc gggaaagagg
      601 tcaggacggc cgacatcgcc gacaaagaga gtatcgccgc agaaaagggc gacaggggta
      661 tcaccgcggg aggtatcggt tgcgatatac gagacatgct caggagtgtg gcctgcggtc
      721 tcgatcaccc tgaagtggat gtgctcaagg ggtatctcgt caccttcaac gagtgcatta
      781 tgaggaaatt tacatttgcc ggactctggc gcatatatct ctgcgccggt tgcctcggca
      841 aggtcaaggt gaccggatac gaagtctgca tgcaggtgag tctccaggat atgggtgatt
      901 cgaagcccca tctcgcgtgc agcagagatg tagcgaccca cgtcgcgcga tggatcgaca
      961 accgcacacg tcctgtttcc cgcaacgatg taagaactgt gggcaatgcc gggaatgaaa
     1021 aactgctgga ttatcattac gctcctcagc ggtctatgcg atgaggagat agatgagtgt
     1081 tacggcgact ccgatgaaga tcaccgtcat gacacttcca gctttgatga aatcctttgt
     1141 cgaataccct cccggaccca tgaggagtgc attgacctga tgggtgggga gaatgaatga
     1201 gttcgatgcg catactgcaa ccaggagggc aagggcgcgg ggatccacgc cggtcccggc
     1261 cccggcaatc atcaccagcg ggacgagcag gacagttgcg gcaacattgg agatgacaag
     1321 ggagagcact gtcgcaagga ctgccacggc acacatgata acaaggggat gcgcaccgct
     1381 gagactcccg atgagggccc ccgcgacgag cgctgccgca ccggtcttct ccatcgcaat
     1441 accaagaggg atcaggccgg caatcagggc gattgtcttc cattcgattg cccggtacgc
     1501 ctcgtcgatt gtcagcaccc ctgaaacgac catcgccgcc gaaccggtga gaagggcaag
     1561 tgacaggggg actccggtca tggtgagggc aagggacgag gcaaatatca gcaccgcgag
     1621 gtgcgccatg ccgtgccgca ccgcgtcacc ttcgggatgg gtgag
//
LOCUS       DAOQVJ010000412         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_97989_length_1663_cov_3.267413, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000412 DAOQVJ010000000
VERSION     DAOQVJ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97989_length_1663_cov_3.267413"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PKL26_07955"
     CDS             complement(<1..1159)
                     /locus_tag="PKL26_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNS83667.1"
                     /translation="MSETVTRPKMSPKDLMIVLVISLGSFMAGLDATIVNIALPTISK
                     AFDVSTVTSSWVLNAYLIVLVSLLLASSRLGDIRGYRGVFLAGFLVFTVGSGLCGLAS
                     GISVLIASRMLQAVGGAIIAALGAVMVTSYLSPSLRGQALGIVAMFTMLGAALGPVLG
                     GFLTSAFSWRFIFYVNLPVGILAILLGMHYLPRKNPVAPSAKLDIPGVMLVFLALGTL
                     IYGLTTLQGERGLLGALSLSGSLVFWILFVIREKRAAEPLLNLALFRNRQFTLQNFNV
                     MCIQLAMAGVMILMPFYLEMVKHLPTDNAGTILLALPIGMILTSPLAGRISDVIGTKK
                     PIILGFVIGTLALFLLATLGPQSSIGHAEIYLFLLGAGSGFAYASLNSAVMG"
     gene            complement(1275..1613)
                     /locus_tag="PKL26_07960"
     CDS             complement(1275..1613)
                     /locus_tag="PKL26_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS83668.1"
                     /translation="MICTKVSETASQPNPHHVDARKVYDSPHAVAVVITLQPGESLKK
                     HATPVDVFFYVLEGTGIVEIGQERETVGKDTLVESPARIPHRWINESRGVFRVLVVKV
                     PRPTEETKLL"
BASE COUNT          384 a          465 c          497 g          317 t
ORIGIN      
        1 cccccatcac cgcactgttg agcgaagcgt atgcaaatcc tgaccctgca ccgagaagaa
       61 agaggtagat ctcggcatgg ccgatgctgg attgtggtcc cagtgttgcc aggagaaaga
      121 gtgcgagtgt cccgatcacg aatcccagaa ttatgggctt cttcgtgccg atgacatctg
      181 agatcctgcc ggcgaggggg gaggtgagga tcatcccgat gggaagggca agcaggatcg
      241 tcccggcgtt atccgtgggc aggtgcttga ccatctcgag atagaatggc atcaggatca
      301 tgacaccggc cattgccagc tgaatacaca tgacattgaa gttctgcagg gtgaactgcc
      361 tgttccggaa gagcgccagg ttgaggaggg gctcggcggc cctcttctcg cgtataacga
      421 agaggatcca gaataccagc gatcccgaaa gtgacagggc ccccaggagt cctcgttcac
      481 cctgaagagt cgtaaggccg taaatgaggg tgccgagtgc aaggaagacc agcatcacac
      541 caggtatatc gagttttgcg gacggtgcga ccgggttctt cctggggagg tagtgcatcc
      601 ccaggaggat tgccagaata ccgaccggca ggttcacgta gaaaataaac ctccacgaga
      661 aggcactggt aagaaagccg cccagcacgg gtccgagtgc cgccccgagc atcgtgaaca
      721 tggccacgat cccgagcgcc tggccccgca gcgagggcga gaggtatgac gtgaccatga
      781 ccgccccgag tgccgcgatg attgcgcctc ccactgcctg gagcatccgg gaggcgatga
      841 gcacactgat accagacgca agtccgcaga gtcctgaccc aacggtgaac accaggaaac
      901 ctgcaaggaa gacaccccgg taacccctga tatcaccgag gcgtgatgat gcgagaagaa
      961 ggctcacaag gacgattaga tacgcattga gaacccatga cgaggtcacc gttgagacat
     1021 caaacgcctt cgagatcgtg ggcagcgcaa tgttcacgat ggtggcatca agccctgcca
     1081 tgaaagaccc aagggagatt acaaggacaa tcatcaggtc tttcggggac atcttcggac
     1141 gggtcacggt ttcactcatg ttctcatcaa ctcatcacaa caattgataa tatagtctgt
     1201 gaatcgagtg tatgatggat cccggcccag ggattaccag atcggggaaa aagcgggtat
     1261 ggctgccgga aagatcacag gagttttgtc tcttccgtcg gccgcggcac cttcactacc
     1321 aggacccgaa agaccccgcg gctctcgttg atccaccggt gggggatcct cgccgggctc
     1381 tccacgagcg tgtccttccc gacggtctct ctctcctggc cgatctcgac gatgcccgtc
     1441 ccctccagta cgtagaagaa gacatccacc ggggttgcgt gcttttttag ggattcgccc
     1501 ggctggaggg tgatcaccac cgccactgcg tgcggggagt cgtagacctt ccgggcgtcc
     1561 acgtggtgag ggttgggctg tgaggcagtt tcgctgacct tcgtgcatat catgacggtg
     1621 ttcctcacac cacctggtgc gtcacaggat ttatcggttc cgc
//
LOCUS       DAOQVJ010000413         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_98087_length_1662_cov_3.415681, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000413 DAOQVJ010000000
VERSION     DAOQVJ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98087_length_1662_cov_3.415681"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKL26_07965"
     CDS             <1..538
                     /locus_tag="PKL26_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNS83669.1"
                     /translation="SYRELTPGRAREVFVELGSPVIVKEPSTSFSLRVEKVEDPAALL
                     QVARRYFKLSDWVVAQEYVESTYDWRVGVLRGRFLYACKYIIPSQTFKIQDSVNGHIV
                     YCGVESVPPAMVPPAVIDLGVRAGQSIGNGLYGVDIKEGPAGPCVIEVNDNPSLEGGE
                     DTAYPHVYRDIVHTLLET"
     gene            550..1053
                     /locus_tag="PKL26_07970"
     CDS             550..1053
                     /locus_tag="PKL26_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNS83670.1"
                     /translation="MMRSFEETAEAICAECGGRCCHEAHPPLSPARMEEFRARGVPLS
                     VAEFAGYTRMKSHDDGMCILCREGKCRVHAFKPETCVAGPFTFEVQDHTLRLFLKHES
                     VCPLVPYLKADEIAYASQFRMAVRSLTALVRSLPAHELDAINRIPEPETDLVAEILLE
                     PEGAGTA"
     gene            1050..>1662
                     /locus_tag="PKL26_07975"
     CDS             1050..>1662
                     /locus_tag="PKL26_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNS83671.1"
                     /translation="MKTGTEHEYSLNGPGFVPLPESDRVLAEIAGSWDGEMPLGGAKA
                     CKELQKHVLEVIPLHPSTRLSDLESSVLGGIREFFRRFGDRYTLLGLGMHPTLTLDQA
                     RVWDHGEGEYYEAYDRLFSIRQHGWLNIQALQVNLEYTNEERLVSLHNRTRCLIPYLV
                     AVSAASPFVEGKAPGPVDNRLLFYRENQARIPAICNGILPEPLS"
BASE COUNT          292 a          553 c          526 g          291 t
ORIGIN      
        1 ctcttaccgg gaactgacgc cggggagggc gcgggaggtc ttcgtggagc tcgggtcgcc
       61 cgtcatcgtc aaggagccct cgacctcctt ctcgctccgg gttgagaagg tggaggaccc
      121 tgccgcgctc ctccaggtgg ccaggcgcta cttcaagctc tctgactggg tcgtcgcgca
      181 ggagtacgtg gagagcacct acgactggcg tgtgggtgtg ctccggggac ggtttttgta
      241 cgcctgcaag tacatcatcc cctcccagac cttcaagatc caggactcgg tgaacggcca
      301 catcgtctac tgcggggtcg agagtgtccc ccccgcgatg gtcccccctg cggttatcga
      361 cctcggcgtc cgggcaggcc agtccatcgg caacgggctc tacggggtgg acatcaagga
      421 agggccggcc ggcccctgcg tcatagaggt gaacgacaac ccgtcgctcg agggcggcga
      481 ggacactgcg tacccccacg tgtaccggga tatcgtccat acgcttctgg agacgtgaag
      541 gagtcagcca tgatgcgatc ctttgaagag acagcagagg ccatctgtgc agagtgcggg
      601 gggcggtgct gccacgaggc gcatcccccc ctctcccccg caaggatgga ggagttccgc
      661 gcgaggggag tgccgctttc ggtggcagaa ttcgccgggt atacccgcat gaagtcgcac
      721 gacgacggga tgtgtatcct gtgcagggaa gggaagtgcc gggtccatgc cttcaagccc
      781 gagacctgcg tggcggggcc cttcaccttc gaggtgcagg accacaccct ccgcctcttc
      841 ctcaagcatg aatccgtctg ccccctggtc ccctacctca aggcggacga gattgcatat
      901 gcctcccagt tcaggatggc ggtgcggagc ctgacagcgc tggtccgatc cctccccgcg
      961 cacgaactgg acgcgatcaa ccggatcccc gagcccgaga ccgatctcgt ggccgagatc
     1021 ctccttgaac ccgagggagc agggactgca tgaagaccgg cacggagcac gagtactccc
     1081 tcaacgggcc gggttttgtc ccgctcccgg agtcagaccg tgtgctcgca gagatcgccg
     1141 ggagctggga cggcgagatg cctcttgggg gtgcaaaggc ctgcaaggag ctgcagaagc
     1201 acgtgctcga ggtgatcccc ctgcaccctt cgacgaggct gtcagatctc gaatcatcgg
     1261 tcctgggggg gatccgcgaa ttctttcgcc ggttcggcga ccgctacacg ctgcttgggc
     1321 ttgggatgca tcccaccctt accctggacc aggcccgggt ctgggaccac ggcgaggggg
     1381 agtactacga ggcctacgac cgcctcttct ccatccgcca gcacgggtgg ctgaacatac
     1441 aggcgctcca ggtgaacctc gaatacacga acgaggagcg cctcgtctct ctccacaacc
     1501 gcacccggtg cctcatccct tacctcgtcg ccgtctcggc ggcatccccg ttcgtcgagg
     1561 ggaaggcccc cgggccggtc gacaaccgcc tcctcttcta ccgcgagaac caggcacgga
     1621 tcccggccat ctgcaacggg atcctcccgg aacctctctc ga
//
LOCUS       DAOQVJ010000414         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_99196_length_1651_cov_3.548246, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000414 DAOQVJ010000000
VERSION     DAOQVJ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99196_length_1651_cov_3.548246"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKL26_07980"
     CDS             <1..363
                     /locus_tag="PKL26_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNS83672.1"
                     /translation="GLYRYLSFSHRLLEMDTRLGIAGRVMAACLGQAVVRASAVTFLW
                     ACVVYRMAWRYAERAWRLVHIDAGHAGQNLYLAAGQVGCGACAIGAFDDQQAADVLGI
                     DGVNEFVIYCAAIGKKGG"
     gene            complement(372..1166)
                     /gene="trpA"
                     /locus_tag="PKL26_07985"
     CDS             complement(372..1166)
                     /gene="trpA"
                     /locus_tag="PKL26_07985"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNS83673.1"
                     /translation="MTTRIVRAFSARTRPLLVACLVAGDPDYGESLAMMREVDRAGAD
                     IIEVVMPFSDPVADGPVMQQAIQRALSAGMNTDSLFSLIRDFRAESETPLVVLTYANP
                     VVQRGIGAFYRDAGSAGVDGVAVADVPLEEADSFCKAARKAGVDPILFVSQTTSMERL
                     KGITAKAGGFLYLVAALGVTGMRGEVDPATIGCIRKIKEMSSLPVVPGFGISSPGQVQ
                     AYSRAGADGIIVGSAIVREVGDRLGRPGEMRAAVGKVVRSLVAGMA"
     gene            complement(1163..>1651)
                     /locus_tag="PKL26_07990"
     CDS             complement(1163..>1651)
                     /locus_tag="PKL26_07990"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314700.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNS83674.1"
                     /translation="VGGGSNAIGIFTPFLDDGTLLFGAEAGGEGLEGRHGATLCRGRP
                     GVLHGTYTYLIQDAFGQILESHSVSAGLDYPGVGPEHSFLRDKGRVTYRAVTDDEALD
                     SFLYLSRTEGIIPALESAHAVSLARRIAQDLEKDDVLVVNLSGRGDKDVAQVAALGGD
                     SL"
BASE COUNT          297 a          544 c          503 g          307 t
ORIGIN      
        1 ggactttacc gttacctctc attctcccat cggcttttgg agatggacac gcgtttgggg
       61 atcgccggcc gggtgatggc cgcatgcctt ggccaggcgg ttgtccgggc ctccgcggtc
      121 acgttcctgt gggcctgcgt agtctaccgg atggcatggc gttacgcgga gcgggcctgg
      181 cggctggtgc acatcgatgc cggccacgca ggccagaacc tctaccttgc cgccggacag
      241 gtggggtgcg gtgcctgtgc aatcggggcg ttcgacgacc agcaggcggc cgatgtgctc
      301 gggattgatg gcgtgaatga gttcgtcata tactgtgcag cgattggaaa aaaaggcgga
      361 taattgatgg ttcaggccat gcctgccacc agggaccgta ccactttccc gaccgcagcc
      421 ctcatctcgc cgggtcggcc gaggcgatcg cccacctccc ttacgattgc ggacccgacg
      481 atgattccgt ccgcccctgc cctggaatag gcctgcacct ggcccggcga gctgatcccg
      541 aatccgggga cgacagggag cgacgacatc tccttgatct tcctgatgca cccgatcgtc
      601 gcaggatcaa cctcccctct catcccagtg acgccgagtg ctgccacaag gtacaggaaa
      661 ccccctgctt ttgcggtgat ccccttcaac cgttccatcg aggtggtctg gcttacgaag
      721 aggatgggat cgacccctgc cttcctggca gccttgcaga aggaatctgc ctcctcgagc
      781 ggcacgtcgg cgaccgcgac cccgtctacg cctgcactcc cggcatcccg gtaaaaggct
      841 ccgatccccc gttgcaccac cgggttggca tatgtgagga cgacaagcgg cgtctccgat
      901 tcagcacgga agtccctgat caatgagaag agggagtcgg tattcatccc tgccgagagc
      961 gcccgctgga tggcctgctg catgacgggt ccgtccgcga ccgggtccga gaacggcatg
     1021 acaacctcga tgatgtcggc accggccctg tccacctccc tcatcatcgc gagcgattcc
     1081 ccgtaatcag gatcacccgc cacgaggcag gcgacgagca ggggacgcgt acgggcggaa
     1141 aatgcacgca cgatgcgggt ggtcacaggc tatctcctcc gagtgccgcc acctgagcca
     1201 cgtccttgtc gccacggccg gagaggttta cgaccaggac gtcgtccttc tccaggtcct
     1261 gtgcgattct ccgtgcgagc gaaacagcgt gggcggattc gagcgcaggg atgataccct
     1321 ctgtccggga gaggtagagg aacgagtcga gtgcctcgtc atccgtcacc gcgcgataag
     1381 tgaccctccc tttatccctg aggaagctgt gctccggacc cacgcctggg taatcgagcc
     1441 cggctgagac gctgtgcgac tccaggatct ggccgaatgc gtcctggatc aggtaggtgt
     1501 aagtcccatg gagcactccc ggcctcccgc ggcagagcgt agccccatgc cgaccctcca
     1561 ggccctcccc tccggcctct gcaccaaaga gaagcgttcc gtcatcgagg aacggggtga
     1621 agatcccgat ggcattggac cctcctccca c
//
LOCUS       DAOQVJ010000415         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_99337_length_1650_cov_2.354232, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000415 DAOQVJ010000000
VERSION     DAOQVJ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99337_length_1650_cov_2.354232"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PKL26_07995"
     CDS             <1..1413
                     /locus_tag="PKL26_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNS83675.1"
                     /translation="GLDHAKKWEFKPLAKEGASVAPGSIIGEVQETNIVHRIMVPPNV
                     KGGTVKSIKAGSFTVDEVVCTLDNGAQISLMQKWPVRVPRPVNEKMNPTVPLITGQRI
                     LDGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTEF
                     PELEDPKSGKPLMERTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYDVSLMADST
                     SRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFYERAGRVKTLAGSMGSVSVIGAVS
                     PPGGDFSEPVTQNTLRIVKVFWALDAKLSQRRHFPAINWLNSYSLYLDSLHDWYDKEV
                     SPEWNTIRSWAMEILQKEAELQEIVQLVGSDALPEAEQITIEVARMIREIFLQQNAYD
                     AVDTFCDMKKQYDMMKAIRQYSDLAYAAQGAGVTPQQIVSIKSKNELPQIKFIKDYKP
                     ALDKIQAEMDAEFNALRSAA"
     gene            1410..>1650
                     /locus_tag="PKL26_08000"
     CDS             1410..>1650
                     /locus_tag="PKL26_08000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNS83676.1"
                     /translation="MKEYRTITQIAGPLVFVEKTEPVGYGELVNIVLFDGSTKRGQVL
                     DTSDELVVVQVFETTAGIGKDSGIRFTGETIKMPVG"
BASE COUNT          379 a          478 c          501 g          292 t
ORIGIN      
        1 ggtcttgatc atgcgaagaa gtgggagttc aaaccgctgg caaaagaagg cgcaagcgtc
       61 gcgccgggaa gcatcatcgg ggaagtccag gagacgaaca tcgtccaccg tatcatggtc
      121 ccaccaaacg tgaagggcgg tacggtcaag agcatcaagg ccggatcctt cactgtcgat
      181 gaggtggtgt gcacccttga caacggcgcc cagatctcac ttatgcagaa atggcctgtc
      241 cgtgtcccgc ggccggtaaa cgagaagatg aacccgacgg ttcctcttat taccgggcag
      301 cggatcctcg acgggctctt tccaatcgcc aagggcggga ccgcggcaat ccccgggccc
      361 tttgggagcg gcaagactgt cacccagcag cagctcgcaa agtggagcga tgcggagatc
      421 gtggtgtaca tcgggtgcgg ggagcgcggg aacgagatga ccgaggtgct gaccgagttc
      481 ccggaactcg aggacccgaa gtcaggaaag cccctgatgg agcggacggt gctcatcgcc
      541 aacaccagca acatgccggt ggccgcacgc gaagcgtctg tttataccgg gatcaccatc
      601 gccgagtatt tcagggacat ggggtacgac gtctcgctga tggccgactc cacctcccga
      661 tgggcggaag ccatgcgtga gatctcctcc cgtctcgagg agatgcctgg tgaagaaggg
      721 taccccgcat acctggcagc gcgcctctcc gagttctatg agagggccgg gagagtaaag
      781 acccttgcgg ggagcatggg atcagtatcg gtcatcggtg cggtctcgcc tcccggtggt
      841 gacttctccg agcctgtcac ccagaacacg ctccgtatcg tgaaggtctt ctgggccctt
      901 gacgcaaagc tctcccagcg ccggcatttc ccggcaatca actggctcaa ctcctactcg
      961 ctctatcttg acagcctgca cgactggtat gacaaggaag tgtctccgga gtggaacacc
     1021 atccgctcat gggccatgga gatcctccag aaggaggctg aactccagga gatcgtacag
     1081 cttgtcgggt cggacgcact tcccgaagca gagcagatca ctatcgaggt ggccaggatg
     1141 atccgcgaga tcttccttca gcagaacgcc tacgatgcgg tggacacctt ctgcgacatg
     1201 aagaagcagt acgacatgat gaaggccatc cgccagtatt ctgacctcgc ctacgcggca
     1261 cagggcgccg gggtgacccc gcagcagatt gtctccatca aatccaagaa cgagctgccc
     1321 cagatcaagt tcatcaagga ctacaagcct gcgctggaca agatccaggc tgaaatggac
     1381 gcagaattca atgcactgag gtcggcggca tgaaagaata cagaaccatc acccagatcg
     1441 ccggtcccct cgtcttcgta gagaagacag aaccggtagg gtacggcgag ctggtcaaca
     1501 tcgtgctctt tgacggctcc acaaagcggg gccaggtgct cgataccagc gacgagctcg
     1561 tggttgtcca ggtcttcgag accactgcag gaatcggaaa ggacagcggg atccgcttca
     1621 ctggtgagac gatcaagatg ccggtcgggc
//
LOCUS       DAOQVJ010000416         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_99928_length_1644_cov_5.032725, whole genome shotgun sequence.
ACCESSION   DAOQVJ010000416 DAOQVJ010000000
VERSION     DAOQVJ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99928_length_1644_cov_5.032725"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..941)
                     /gene="htpX"
                     /locus_tag="PKL26_08005"
     CDS             complement(63..941)
                     /gene="htpX"
                     /locus_tag="PKL26_08005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNS83677.1"
                     /translation="MKVWKRDFGLTLRIWLTLFLLFLVYLVFLAVLSWLGVGLPFLVL
                     LVFGMAFIQYFFSDKLVLWSTRARVIEPEEYPELHRMVQRLADEAGIPKPRVAIMQTP
                     VPNAFATGRSPKHAVVAVTDSIMRLLTPRELEAVLAHELSHVKNRDVLTLTIASFVAM
                     LAALIMNNFLFASLFSRRDDNPWIIAGIVAIVVYFVAQLLIMALSRYREFAADRGSAY
                     LTGRPADLISALEKISGRMDRVPAEQKRVVESANAFFIIPALSGSTLMELFSTHPPLE
                     KRIAALEKVEMEMRGY"
     gene            1125..>1644
                     /locus_tag="PKL26_08010"
     CDS             1125..>1644
                     /locus_tag="PKL26_08010"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNS83678.1"
                     /translation="MELDEITISRAILSSQVEKLLEFMEMDVAVVGGGPSGLTAAALI
                     GEQGFRVGLIEKKLSVGGGMWGGGMMFPRIVVQEEAKRLLDQFDIAHTSYTEGYYVAS
                     SVEAVSKLTASACDAGVEFFNLFSVEDVMIRGDTRLSGLVVNWTPVEMAGLHVDPLTM
                     GCRVAVDATGHDA"
BASE COUNT          366 a          449 c          524 g          305 t
ORIGIN      
        1 gggccgctga tgagaggact caaaaaggaa aaatatgccg ggtgtgggat actgcgaggg
       61 ggttagtacc ccctcatctc catctccacc ttctcgaggg cggcgatacg cttctcgagc
      121 gggggatgcg tggagaagag ctccatcagg gtgctgccgg agagtgccgg gatgatgaag
      181 aacgcattgg cgctctcgac cacccgcttc tgttccgccg ggacgcggtc catccgcccg
      241 ctgatcttct cgagggcgct gatcaggtca gccggcctcc cggtcaggta ggcgctcccc
      301 cggtccgcgg caaactcccg gtagcgggag agtgccatga taaggagctg ggccacgaag
      361 tatacgacga ttgcaacaat ccctgcgatg atccagggat tgtcgtccct tcgggagaag
      421 aggctcgcaa agaggaagtt gttcatgatc aatgctgcga gcatggcaac gaaactggcg
      481 atggtcaggg taaggacatc ccggttcttc acgtgggaga gttcgtgcgc gaggaccgcc
      541 tcgagctcgc gcggggtaag gagccgcatg atggaatcgg tcaccgccac cacggcatgt
      601 ttcgggctgc gcccggtcgc aaacgcattt ggcaccggcg tctgcatgat ggcaaccctc
      661 ggcttcggga tgcccgcctc atcggccagc ctctgcacca tgcggtgcag ctcgggatac
      721 tcttcgggct cgatcacccg ggctctcgta ctccagagca cgagcttgtc cgagaagaaa
      781 tactggatga aggccattcc gaacaccagg agcacaagga acggaagtcc cacgcccagc
      841 catgagagga ccgccaggaa gaccaggtac acgagaaaca gcaggaacag ggtcagccag
      901 atcctcagcg tgagtccgaa gtcgcgcttc catactttca taatgagtac ttttctgtaa
      961 ccgacgacat aaacattctc ccagaggcag gaaaaaaacg ggaaacgccg caaaaaaggg
     1021 agaaaagata ttatagcagg cgaaaggccc cgcatgggat ttgccgccgc acctttatgc
     1081 gtatatttac agtggagagg gtacaaagta tgtgatacga taccatggag cttgacgaga
     1141 tcacgataag cagggcgatt ctctcctcac aggtggagaa gctgctggaa ttcatggaga
     1201 tggacgttgc agtcgtggga ggaggccctt caggcctcac tgccgccgcc ctcatcgggg
     1261 aacagggctt tcgggtcggc ctgatagaga agaaactttc cgtgggcggc gggatgtggg
     1321 ggggaggcat gatgtttccc cgcatcgtgg tccaggaaga ggcaaagcgg ctcctggacc
     1381 aatttgacat cgcccatacc tcctacaccg agggatatta tgtggccagc tcggtggagg
     1441 cagtctcgaa actgaccgct tctgcatgcg atgcgggggt ggagttcttc aacctctttt
     1501 cggtcgagga cgtgatgatc aggggcgaca cccgcctctc ggggctggtc gtgaactgga
     1561 caccggttga gatggccggt ctccacgtgg accccctcac catgggatgc agggtggccg
     1621 tggatgccac gggtcacgac gcgg
//
LOCUS       DAOQVJ010000417         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_100990_length_1634_cov_4.075997, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000417 DAOQVJ010000000
VERSION     DAOQVJ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100990_length_1634_cov_4.075997"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKL26_08015"
     CDS             <1..277
                     /locus_tag="PKL26_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HNS83679.1"
                     /translation="WRDVAVKLEAPARNYAEVNLSKINRYAGDGETIVVPGKVLGSGM
                     LETSVKIAALTFSESAATKILEANGQCLTIEELVKDNPKGSRIRILR"
     gene            284..706
                     /locus_tag="PKL26_08020"
     CDS             284..706
                     /locus_tag="PKL26_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNS83680.1"
                     /translation="MTTIINGEGLLLGRMASIVAKRALNGETIAIVNAEKAIISGSRA
                     RVLSLYGQKRSRGSREGGPFFPRRPDHIVRRTIRGMLPYKRAPGMEAFKRVKVYVGIP
                     IDLVGQEMEVLDEAHVNRLNTPKTVTVGAVSTFLGARY"
     gene            721..1122
                     /locus_tag="PKL26_08025"
     CDS             721..1122
                     /locus_tag="PKL26_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNS83681.1"
                     /translation="MVKIVNTSGKRKTAIARATVRPGKGRVRINSVPLDFYPNEMVRL
                     KIAEPILLAPKAIEGIDISIDVKGGGMMGQAEAVRTALARGIYRWSNDPQIKDVFLSY
                     DRTLLVNDSRQKESKKPHGRGARKKFQKSYR"
     gene            1155..1343
                     /locus_tag="PKL26_08030"
     CDS             1155..1343
                     /locus_tag="PKL26_08030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HNS83682.1"
                     /translation="MIPVRCFTCGKVISPAYEEFKRRKEAGEDPKKILDDLGIERYCC
                     RRMFLAHKEIIDDLNPYQ"
     gene            1344..1418
                     /locus_tag="PKL26_08035"
     tRNA            1344..1418
                     /locus_tag="PKL26_08035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1380..1382,aa:Pro,seq:cgg)
     gene            1450..1620
                     /locus_tag="PKL26_08040"
     CDS             1450..1620
                     /locus_tag="PKL26_08040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HNS83683.1"
                     /translation="MKETYTRYERARIIGARALQISMGAPLLIQTGKIDPLEIALEEF
                     QHNVIPITVKRK"
BASE COUNT          410 a          407 c          519 g          298 t
ORIGIN      
        1 atggcgggat gtggcggtga agctggaagc gcctgcccgc aattatgcgg aggtcaacct
       61 gagcaagatc aaccgctatg cgggcgatgg agagaccatc gtggtcccag gcaaggttct
      121 cgggagcggg atgctggaga catcggtgaa gatcgcggcg ttgacatttt cagagtcggc
      181 ggcaacaaag atcctcgagg cgaacggcca gtgcctcacg atcgaggaac tggtgaagga
      241 caaccccaag ggaagcagga tcaggatact caggtgaggt gagatgacca caataatcaa
      301 cggcgaaggc ctccttctcg ggagaatggc cagtatcgtg gcaaagcgcg ccctgaacgg
      361 cgagaccatc gccattgtca atgccgaaaa ggccatcata tccgggagca gggcgagggt
      421 gctttcgctc tacggccaga agaggagcag ggggtcacgc gaagggggac cgttcttccc
      481 gcgaaggccc gaccacattg tcaggcggac gatcaggggg atgcttccct acaagcgggc
      541 tccaggcatg gaggcgttta agcgggtgaa ggtctatgtg ggaatcccga tagacctcgt
      601 cggccaggag atggaagtcc tcgacgaggc gcacgtaaac cggctcaaca cgccaaagac
      661 ggtgacggtt ggtgcggtga gcacgttcct tggagccagg tactaggagg aatcagaggc
      721 atggtaaaga ttgttaatac cagtggaaag aggaagactg caatcgcaag ggcgacggtc
      781 cggccgggaa agggcagggt gcggatcaac tcagtgccgc tcgacttcta cccaaacgaa
      841 atggtgcggc tgaagatcgc cgagcccatc ctcctggccc cgaaggccat cgaggggata
      901 gacatctcca tcgatgtcaa gggcggtggc atgatggggc aggcggaagc ggtgcgcact
      961 gcccttgcac ggggaatcta ccggtggtca aacgaccccc agattaagga cgtcttcctc
     1021 tcgtacgaca ggacgctcct cgtgaacgat tcccggcaga aagaatcgaa gaaaccccat
     1081 ggcaggggtg cgagaaagaa gttccaaaag tcttatcgtt gagatatgga aattatctgg
     1141 gagatcatac cggcatgata cccgtacgat gtttcacctg tgggaaagtc atctcacctg
     1201 cgtatgaaga gttcaagcgc cgcaaggagg ccggcgagga tccaaaaaag atcctcgacg
     1261 accttggcat agagcgctat tgctgccgca ggatgttcct tgcccacaaa gagataatcg
     1321 atgatctcaa tccgtaccaa tgaggggtcg tggggtagcc tggactatcc tatggcgttc
     1381 gggatgctgt gacctgagtt cgaatctcag cgaccccatt tatcgcgcat ggatacattt
     1441 tgagggtata tgaaggagac ttatactcgg tatgagcggg ccaggatcat cggagccagg
     1501 gcacttcaga tatcgatggg tgcccccctg ctgatccaga ccggcaagat cgatcctctg
     1561 gagatagctc ttgaggagtt ccagcacaac gtcataccta tcacggtgaa gaggaagtag
     1621 tcagccatga cggt
//
LOCUS       DAOQVJ010000418         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_101649_length_1628_cov_4.237762, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000418 DAOQVJ010000000
VERSION     DAOQVJ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101649_length_1628_cov_4.237762"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..464
                     /locus_tag="PKL26_08045"
     CDS             69..464
                     /locus_tag="PKL26_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNS83684.1"
                     /translation="MAGEKEKWGIAHIFASFNNTIITVTDLSGAETVCKSSGGMVVKQ
                     DRNESSPYAAMQMASNVAQTAMEKGIVGVHVKVRAPGRGKQRSPGPGAQAAIRALARA
                     GMRIGRIEDVTPVPHDSIRQKGGRRGRRV"
     gene            464..1309
                     /locus_tag="PKL26_08050"
     CDS             464..1309
                     /locus_tag="PKL26_08050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HNS83685.1"
                     /translation="MEIVFSSLDDSSARFRLSGTSQPFANALRRAMIGEVPTLAIEDV
                     RIYDNTSALFDEMLGHRLGLIPIRTDLDCYIPQEQCTCEGAGCDRCSVMFTLSVEGPA
                     MVYSRDLIPQDPRAAPAVDSIPIVKLAKDQKVVLEARAVLNRGRVHAKWQPTTTCGFK
                     NSPVLEISEKCDACGQCVDECPRGILVVRGGKVTVADGRLEECSLCRLCERACLNTGI
                     GEEPAIRVRSEDSRFIFVVEGDGSLPVREILERALFYLKSQSEELCEKIGEVSGVTGD
                     EEENE"
     gene            1290..>1628
                     /locus_tag="PKL26_08055"
     CDS             1290..>1628
                     /locus_tag="PKL26_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HNS83686.1"
                     /translation="MKRRTNKKTNPRLVNLISLLKDTSRQNEVNVWRDVAVRLEAPAR
                     NYAEVNLSKINRYAGDGETIVVPGKVLGSGMLETSVKIAALTFSQSAATKILEANGQC
                     LTIEELVKDNP"
BASE COUNT          377 a          433 c          540 g          278 t
ORIGIN      
        1 gcaggtcgcc tgttgcgaat gccgatcacg ccgagagggc acgcatcgcc aggacaggga
       61 ggtaaggaat ggcaggcgag aaggaaaaat ggggaattgc gcacatattc gcgtcattca
      121 ataataccat catcaccgtc accgacctct cgggggccga aacggtgtgc aagagcagcg
      181 gcggcatggt ggtcaagcag gacaggaacg agagctcccc gtatgcagcc atgcagatgg
      241 catcgaacgt tgcccagacc gcaatggaga aggggatcgt aggcgtgcac gtgaaggtcc
      301 gtgcccccgg aaggggaaag cagcgcagcc cggggccggg tgcccaggca gccatccgcg
      361 cactggcccg cgccggcatg cgcatcgggc ggatagagga cgtcactccg gtcccccacg
      421 actctatccg ccagaaaggc ggccggaggg gcaggagagt ctgatggaga tagtgttctc
      481 aagccttgat gactcttcgg caaggttcag gctttcgggg acgagccagc cgtttgccaa
      541 cgcgttgcgc cgggcaatga tcggagaagt gcccacgctt gcaatcgagg acgtgcgcat
      601 ctatgacaac acgagcgcac tcttcgacga gatgcttggg catcgccttg ggctgatccc
      661 catccgtacc gacctggact gctatattcc gcaggagcag tgcacctgcg aaggggcggg
      721 ctgtgaccgg tgcagtgtta tgttcactct ctccgttgag gggcccgcca tggtgtattc
      781 acgggacttg ataccacagg acccgcgcgc agcacctgcc gtagatagta tccccatcgt
      841 caagctcgca aaagaccaga aggtggtcct cgaagcaagg gcagtcctca acagggggcg
      901 ggtgcatgcc aagtggcagc cgaccaccac ctgcgggttc aagaattccc ctgtgctgga
      961 gattagcgaa aagtgcgatg cctgtggaca gtgcgttgac gaatgcccaa ggggcatcct
     1021 cgtcgtcagg ggcggaaagg taacagttgc agatggaagg ctggaggaat gctcgctctg
     1081 ccgcctctgc gagcgggcct gcctgaatac cggcatcggc gaagagcctg ccatccgtgt
     1141 caggtctgaa gattcgaggt tcatattcgt ggtagagggg gacggctccc tccctgtaag
     1201 agagatactg gaaagggcgc ttttctacct gaagagccag tcggaagagt tgtgtgagaa
     1261 gataggtgaa gtatcgggag tgaccggaga tgaagaggag aacgaataag aagaccaatc
     1321 cccgcctggt aaacctgatt tccctgctga aggatacttc ccggcagaac gaggtgaacg
     1381 tatggcggga tgtggcggtg aggctggaag cgccggcccg caattatgcg gaggtcaacc
     1441 tgagcaagat caaccgctac gcgggtgacg gagagaccat cgtggtccca ggcaaggttc
     1501 tcggaagcgg gatgctggag acatcggtga agatcgcggc gctgacattt tcgcaatcag
     1561 cggcaacaaa gattctcgag gcgaacggcc agtgcctcac gatcgaggaa ctggtgaagg
     1621 acaacccc
//
LOCUS       DAOQVJ010000419         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_101867_length_1626_cov_3.438574, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000419 DAOQVJ010000000
VERSION     DAOQVJ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101867_length_1626_cov_3.438574"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKL26_08060"
     CDS             complement(<1..361)
                     /locus_tag="PKL26_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNS83687.1"
                     /translation="MRVAVVEGDGIGHEVIPVAKRALEVLRPDMEFFPVEVGYGRWER
                     TGQAITEDEIDALASADTILFGAVTTPPSPDYRSVIVRIRKELDLYANLRPVWGEGFD
                     ILVVRENTEGLYSGVEKI"
     gene            complement(358..1161)
                     /locus_tag="PKL26_08065"
     CDS             complement(358..1161)
                     /locus_tag="PKL26_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83688.1"
                     /translation="MIFPRLCKEVGYASSRPCGDKVYFLSRFLVRETEDGPELLEVHL
                     DEKGPGLMRAIRNECVLAAPGEVTVYPEKVQIHDRALLVRLAMESGTRCTIFTGLDEH
                     STFVLDPDIGAFQTVHVYDVVPPRPSLSSAIRELEATGLFGDLDVVFEHSLQDISLQQ
                     ADVYPCRAAGFTHTLDADPLEGGERVAGCMTGAQLIRECYGDDFDIVEICPLKMVKDE
                     PFIARCCRKEREGVGVYNGKTGAVVHWGANPAQIYHSVCRLLERWRERA"
     gene            complement(1158..1625)
                     /locus_tag="PKL26_08070"
     CDS             complement(1158..1625)
                     /locus_tag="PKL26_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNS83689.1"
                     /translation="MIEGHALCLGPDIDTDVIIAGRYLRTRDRSEWARHAFEDLDPTL
                     SSRLEGTVILAGKNFGCGSSREQAAIALKEAGVRGVIAPSFARIFYRNAINVGLPVIE
                     CECTCTDGEMVRADPAAGKVAIGDRSYLTRPLSPRMREILSAGGLVSYWRGRR"
BASE COUNT          294 a          556 c          469 g          307 t
ORIGIN      
        1 cgatcttctc caccccggag tacagcccct cggtattctc gcgcacgacc aggatgtcga
       61 acccttcgcc ccacacaggc cggaggttcg cgtagaggtc cagttctttc cggatgcgca
      121 cgattacgct ccggtaatcg ggcgaggggg gagtggtgac cgcaccaaag aggatggtgt
      181 cggcggacgc gagagcatcg atctcatctt ccgtgatagc ctggcctgtc cgctcccacc
      241 tcccgtatcc cacctcgacc gggaagaact ccatgtcagg ccggaggact tcaagcgccc
      301 tctttgccac cgggatgacc tcgtgcccga tcccgtctcc ctcgacgacc gctaccctca
      361 tgccctctcc ctccagcgct cgaggagtcg gcagactgaa tggtaaatct gcgcaggatt
      421 cgcaccccag tggaccaccg cgccggtctt cccgttatag actccgaccc cctcccgttc
      481 cttccggcag cagcgggcaa taaacggttc atcttttacc atcttcagcg ggcagatctc
      541 gactatgtcg aaatcatccc cgtagcattc ccggatcagc tgggcaccgg tcatgcaccc
      601 tgcaaccctc tctcctccct ctagggggtc ggcgtccagg gtgtgcgtga atcctgctgc
      661 cctgcacggg tacacgtctg cctgctgcag ggatatgtcc tggagtgaat gttcgaacac
      721 gacatcgaga tcgccgaaca gcccggttgc ttcgagttca cggatcgcgg acgagaggct
      781 gggccgggga ggtacgacat catagacatg caccgtctgg aacgcaccga tgtcagggtc
      841 gagcacgaac gtggagtgct cgtcgaggcc ggtgaagata gtgcaccggg tgcccgattc
      901 cattgccagg cggaccagca gggcccgatc gtggatctgg accttctccg ggtacacggt
      961 cacctctccg ggagcagcga gcacgcactc attcctgata gcccgcatca ggccgggccc
     1021 tttttcgtcc aggtgcacct ccaggagctc cggcccgtct tctgtctccc tgacgaggaa
     1081 ccgggagaga aaatacacct tgtccccgca gggccgggaa gacgcgtatc ccacctcctt
     1141 gcacaggcgg gggaagatca cctccggccc ctccagtacg agaccagccc tccggcagag
     1201 aggatctccc gcatccgtgg agagagcggc cgggtcaggt aggaccggtc cccgattgcc
     1261 accttccctg cggccggatc cgccctcacc atctccccat cggtgcaggt gcattcgcac
     1321 tcgatcaccg ggaggccgac gttgatggcg tttctataga atatccgggc gaaacttggt
     1381 gcaatcaccc ccctcacgcc tgcttccttc agggcgatcg cggcctgctc ccgcgaagag
     1441 ccgcacccaa agttcttccc tgcgaggatc accgttcctt ccaggcggga ggagagggta
     1501 gggtccaggt cttcgaacgc gtgccgtgcc cactcggacc tgtccctcgt gcgcaggtag
     1561 cgcccggcga tgatcacatc ggtatcgatg tccggcccga ggcagagtgc atgcccctcg
     1621 atgatc
//
LOCUS       DAOQVJ010000420         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_102014_length_1625_cov_2.600637, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000420 DAOQVJ010000000
VERSION     DAOQVJ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102014_length_1625_cov_2.600637"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKL26_08075"
     CDS             <1..740
                     /locus_tag="PKL26_08075"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNS83690.1"
                     /translation="LCSSVLSPGDSVEISPPTFGEYEFSARLAGASIGRGPGTRLRFL
                     CNPNNPTGHLVERDEILSMIDRCEEDEALLAVDEAFIEISDDPMQTLVGIRHPSLFVM
                     RSLTKSFAVPGIRFGYGFGNPDLVGMLEARRLPWSVNVVAEHLAIEAFLRYEELSISR
                     ERIRSERERLCRGLLDLPLEIVPSRTNFLLLNTKGDVVPLVECLMRRGILVRDCHSFG
                     LPNSIRVAVRTREENLRLLEALSECLR"
     gene            728..1342
                     /locus_tag="PKL26_08080"
     CDS             728..1342
                     /locus_tag="PKL26_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNS83691.1"
                     /translation="MLALILAGGIGSRLALGEKPLVTIDGMPMIMYVIEAFESAGFDV
                     VAVLSRKTPYTHNWCRAHGISHYTAEGMGYVEDIAECADAHGITGPFFTSVADIPCLE
                     RDIVQTLHEVYRHSQKDALSSWIPRGLAERFGSRVAYIEKINGLDACPAGINILRGDC
                     MGTPQDETRVILDDFRLAYNINTREALDLVTRVIRARRNLGRNS"
     gene            1452..>1625
                     /locus_tag="PKL26_08085"
     CDS             1452..>1625
                     /locus_tag="PKL26_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNS83692.1"
                     /translation="MQVSREVELEGHIIDSGIMTRVFDKIMDMGGNFEIIVFNVGKRK
                     SDPSYAHLQVNADT"
BASE COUNT          363 a          414 c          453 g          395 t
ORIGIN      
        1 tcctctgttc atcggtcctc tcaccagggg acagcgtgga gatctcgccg cccactttcg
       61 gagaatacga attttctgca cgactcgcag gtgcctccat cggaagggga cctggcacac
      121 ggctccggtt cctgtgcaat cccaacaatc ccaccgggca tctcgttgag agagatgaaa
      181 tactttcgat gatcgaccgg tgcgaagagg atgaggcact cctggcggtg gacgaggcat
      241 tcatcgagat ctcggatgac ccaatgcaga ctcttgtcgg aatacggcat ccctcactct
      301 tcgtgatgcg atctctcaca aagagctttg cagtgccggg cattcgtttt ggatatgggt
      361 tcggcaaccc ggacctcgtc gggatgctgg aagcccgccg gttgccatgg agcgtcaatg
      421 tcgtggcaga gcatctagcg atagaggcat ttttgcggta tgaagagctg tccatctcgc
      481 gtgaacgcat caggagcgaa cgtgaacgcc tctgcagggg cctcttggat cttcctctcg
      541 agattgttcc ttcccggaca aacttcctcc tcctcaatac gaaaggcgat gtagttcccc
      601 ttgttgaatg cctgatgcgt cgcgggatac ttgtcaggga ttgccactca tttggcctcc
      661 cgaattctat ccgtgttgct gtgcgcacga gggaagagaa tctccgcctc ctggaggcgc
      721 tgtcggaatg cttgcgctga ttcttgccgg tggtattggg agcaggcttg ctctcggtga
      781 gaaacctctt gtcaccatcg acggcatgcc gatgatcatg tacgtgatcg aggcattcga
      841 gtccgcagga ttcgatgtgg tggcggtcct ctccaggaag acgccctata cccacaactg
      901 gtgccgtgcc catgggattt ctcattacac cgcggagggg atgggatacg tggaggacat
      961 tgcagagtgt gcagatgccc atgggataac tgggccgttc ttcacttccg ttgcggacat
     1021 accctgtctt gagagagaca tcgtgcagac tctccacgaa gtctaccggc acagtcagaa
     1081 ggatgcgctc tcaagctgga tcccacgggg ccttgcagaa aggtttggct cacgtgtcgc
     1141 gtacattgaa aaaatcaatg gtctggatgc ctgcccggca ggcatcaata tcctccgggg
     1201 ggactgcatg gggacacccc aggacgagac ccgggtcatt ctggacgatt tccgccttgc
     1261 atataatatc aatacccgcg aggcgcttga tctcgtcacc agggtgatcc gggccagaag
     1321 gaacctgggc aggaattcat gagaccaatg atctaataaa cacgggttca agcctgtttt
     1381 ttatttttca ggaaaatcag ggtttttttc ttgagaagta atttaaatca tcgtggcaga
     1441 aataactgta tatgcaggtc tcgcgggaag tggaactcga aggccatatc attgactctg
     1501 gtatcatgac ccgggtcttt gataaaatta tggatatggg tggaaatttt gagattatcg
     1561 tttttaatgt cggaaaacga aaatctgacc cgagttatgc gcacctgcag gtaaatgcag
     1621 acacc
//
LOCUS       DAOQVJ010000421         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_102350_length_1622_cov_2.435865, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000421 DAOQVJ010000000
VERSION     DAOQVJ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102350_length_1622_cov_2.435865"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKL26_08090"
     CDS             <1..359
                     /locus_tag="PKL26_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS83693.1"
                     /translation="FLSPWSIVLTYGILGIFGVLLSAFSTTVALYLSDHDAYAAFNAM
                     VSMPLFFTSSALMPYDVMPSWLAPLAYWNPVSYAIDAIRALQEGVLAWGRIGALVLLA
                     VLVVAISVRQFRKVTL"
     gene            627..953
                     /locus_tag="PKL26_08095"
     CDS             627..953
                     /locus_tag="PKL26_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83694.1"
                     /translation="MEMTVHEQSNSTLDTDRDTCLFRAPRDPDRIAYLRGKDLYEHRR
                     NDQQSLYYIHRWSLAPGERETLSLISPVMAGRFLESKGIFCPETGDLRGAAILRSWGY
                     GILEEF"
     gene            complement(1120..>1622)
                     /locus_tag="PKL26_08100"
     CDS             complement(1120..>1622)
                     /locus_tag="PKL26_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNS83695.1"
                     /translation="ILARIVDGSEVLVENAAEGLAGLELSSTPSGAMIVIDGFNTGKL
                     TPALVGNLSPGQHRVDLLLPGYYPSSQVVTLVDYRNEQVDMRITVPLDAYYSGSLTIE
                     SDPPGAKISFFSKDTGRKTPATFDCLAIGTVSGRVRWDDAERDFEVDILPGENLTVNL
                     SPPEKK"
BASE COUNT          346 a          478 c          435 g          363 t
ORIGIN      
        1 ccttcctctc accctggagc atcgtcctca cgtatggcat cctgggtatc tttggcgtgc
       61 tcctctcggc gttctcgaca acggttgcgc tctacctctc cgaccacgac gcgtatgccg
      121 cgttcaacgc catggtgagc atgccgctct tcttcaccag cagcgcactg atgccgtacg
      181 atgtcatgcc ctcgtggctt gcccccctcg cctactggaa ccctgtcagc tatgcgattg
      241 acgcgatcag ggcgctccag gagggagtcc tcgcctgggg gaggataggc gcccttgtcc
      301 tcctcgcggt gctggtcgtt gcgatcagcg tccggcagtt ccgaaaagtg acactgtaat
      361 caatacaacg atccagtggc cagacgtgaa cgatcaaaat tctgcgggac cttaactcgc
      421 aactgtgcat aattttttta taagaattta taaaaatcgc acatagtgct acgaatatgc
      481 aaatatttat tacattaacc tgcgtaccac tttactggca gaatcagcca tgtgattctc
      541 tgccgcaggg atcacccgga cttctcgtct tctcgatttc ctgctcacat cgcctcatgg
      601 aaaatgcaca cataggagag aatgtcatgg agatgaccgt gcacgaacag agtaattcca
      661 cactcgatac cgatcgggac acctgcctgt tccgggctcc aagggacccg gaccgcatcg
      721 cgtatctgcg gggtaaagat ctctatgaac acagacgcaa tgatcagcaa tccctctact
      781 acatccatcg ctggtcgctt gcacctggcg agagggaaac tctctcgctc atctctccgg
      841 tcatggcagg ccggttcctt gaatcgaagg gaatcttctg ccccgagacc ggagaccttc
      901 gcggagcggc aatattgcgg tcctggggat atggaatcct ggaagaattc tgaaataccg
      961 ccgcctgact gcgagagtct atccatacgc cgccatcacc gtcccctcag cggggagccg
     1021 ggccaccggt cccccggaag gcggcgtgag gtgtgaattc ccttctactt ctgtcccttc
     1081 caaaaggtgg gggaggatgc tcctgaacag gtgatcgtat cacttcttct caggcggcga
     1141 gagattgaca gtgagattct cacctggcag gatatccacc tcgaagtccc gctcggcatc
     1201 gtcccagcgg acccttccac tgaccgtccc gatcgcgagg cagtcgaatg ttgcaggggt
     1261 ttttctcccg gtatccttgc taaagaagga gatcttcgca ccggggggat cgctctcgat
     1321 ggtgagggac cccgaataat atgcgtccag gggaaccgtg attcgcatat cgacctgctc
     1381 attccgatag tcgacaaggg ttaccacctg cgaggagggg taatacccgg gcaggaggag
     1441 gtcgacgcgg tgctgtcccg gggagaggtt tccgacgagt gcaggtgtca gtttcccggt
     1501 gttgaaacca tcgatgacga tcatggctcc ggagggtgtc gagctcaact cgagccccgc
     1561 aagtccttcg gccgcattct caacaaggac ctctgaacca tccacgatcc gtgcaaggat
     1621 ag
//
LOCUS       DAOQVJ010000422         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_102641_length_1619_cov_2.906010, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000422 DAOQVJ010000000
VERSION     DAOQVJ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102641_length_1619_cov_2.906010"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..838)
                     /locus_tag="PKL26_08105"
     CDS             complement(254..838)
                     /locus_tag="PKL26_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS83696.1"
                     /translation="MIDENNIDSLPLHIRTEPPFYADFTANPVSGNPPLTVQFTDASD
                     GNIMRYLYRFGDGFSSMSQNPVYTYRRPGNYTVSLTIWQMDGRTLSSKTMVKENYIRV
                     EGAPGPDVRTNFSATPVSGTAPLQVAFTGNSSGSPILWKYSFGDGFMSTQKNPTHTYR
                     RPGTYTVKLTVWTIGPDRKLATETIERASYITVA"
     gene            990..>1619
                     /locus_tag="PKL26_08110"
     CDS             990..>1619
                     /locus_tag="PKL26_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNS83697.1"
                     /translation="MKIALVSSLADPGGCTIHGALLSLLATRHEPYPLEQHELSHHRV
                     RERLIYQDHLDRDLDADLIIFLSRHSSVNPVPVLTVHVTGNISSAEFGGQERSLAPAA
                     PEWMHAVLRELSGNAPPGFRVAYEVTHHGPSELATPSLFAEVGSSEKEWRDSAAGMAV
                     ARSVLSADPADCIPLIGFGGTHYAARQTHIALNTRGAFGHIAHTREVSSL"
BASE COUNT          335 a          457 c          471 g          356 t
ORIGIN      
        1 cgcggtctcc gccgatgggg gcctgggcgc ctgccggcag gatggcaagg cagcatcctg
       61 taaccaggaa tacgatgaaa atacgagtag agctgctcat tgtgatcgcc tttttttgaa
      121 taccacgggt atattcgatc cgttcattta aaatggattc catcggtgtg acacaagaaa
      181 aggcgattaa tccctgggag ggaattttag cgatgcagta acccgattat aaaaattgga
      241 atgggagggt ttttcatgcc acggtgatat aactcgctct ctctatggtc tctgtggcaa
      301 gtttcctatc tgggccaatg gtccagaccg tgagtttcac cgtgtaggtc cccggtctcc
      361 ggtaggtatg ggtcgggttc ttctgggtgg acatgaaccc gtcgccgaaa ctatacttcc
      421 agagaatcgg gcttcctgac gaattcccgg tgaaggcaac ctgaaggggt gcagtgcctg
      481 ataccggggt agccgagaaa ttcgtcctga catcgggtcc tggtgcccct tctacccgga
      541 tatagttctc ttttaccatg gttttcgagg acagcgtccg cccgtccatc tgccagatgg
      601 tcaggctcac ggtgtagttg cctggccgcc ggtacgtata cacaggattc tggctcatcg
      661 acgaaaatcc gtctccaaac ctgtagagat aacgcatgat gttcccgtca gaggcatcgg
      721 tgaactgcac ggtgagaggc gggttcccgg agacaggatt cgcagtgaaa tccgcataga
      781 agggcggttc ggtcctgatg tgtagcggga ggctgtcaat attgttctca tcgatcacga
      841 agggtgcgtt gcagaacccg tcagtccgga ccggggcctg ttattgcgag tctattccct
      901 gtgcggtccc attgctggat tgccacgatc acacaccccg gatcgcgtcc ctttatagaa
      961 attgaaaggc atagaatggg aagtaaccaa tgaagatcgc acttgtcagc tcccttgcag
     1021 accccggcgg ctgcaccatc cacggggccc tcctctcgct ccttgccacg cgacacgagc
     1081 cctatcccct ggaacagcac gaactctccc accacagggt cagggaacgg ctgatttacc
     1141 aggatcacct tgaccgggac cttgacgctg atctcatcat cttcctctcc cggcactcct
     1201 cggtgaaccc ggtaccggtc ctgaccgtgc atgtcaccgg gaacatctcg agcgctgaat
     1261 tcggcgggca ggaacgctcg ctggccccag cggcccccga atggatgcac gcggtattga
     1321 gagagttgtc agggaatgca ccgccaggct ttcgtgtcgc gtacgaggtc acccatcacg
     1381 gcccgtcgga actcgcaacc ccctcgctct ttgcagaggt ggggagcagc gagaaggagt
     1441 ggcgggacag cgcggccggg atggcggttg caaggagcgt gctctcggca gatcccgcgg
     1501 actgtattcc cctgatcggg tttggcggga cgcactacgc agcccggcag acccatattg
     1561 ccctgaatac acgcggggcg ttcgggcata tcgcccatac gagggaggtt tcatccctc
//
LOCUS       DAOQVJ010000423         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_102900_length_1616_cov_3.686739, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000423 DAOQVJ010000000
VERSION     DAOQVJ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102900_length_1616_cov_3.686739"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="PKL26_08115"
     CDS             complement(<1..1611)
                     /locus_tag="PKL26_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNS83698.1"
                     /translation="MRIGLVQASAGPDHAGNLEKVSGMVQDAFDRGAQVVCLQELFSA
                     PYFPRVHGADADRYAEHIPGETTRVLGEIARRNQGLIVAPLLEKGAGGRRYNAAVIIR
                     SDGSTGDVYRKVHIPHDPSYYEKEYFSPGSSYVVEETHFGRIAVLICYDQWFPEAART
                     VSLMGAQVIFYPTAIGTVHGMEDPLEGDWEDAWQTVQRGHAIANGVHLAAVNRVGIEG
                     EITFFGGSFVCDAFGNIIAKAGRGEEIIVAEIDLSQNDLVREGWGFFSNRRPDTYGPL
                     VDTGTFPGAEARAPSGFRMPAEWEPHQGIWLSWPHDNDTFFDLPRVEHSYLGMIQALR
                     GRERVYLMVRDQEMRSRVEEILSCAGIEPWHVKFFVYPYCDVWFRDYGPTFLVHARDS
                     SMAMVNWRFNAWGGKYPELEGDDIIPSVLARSLHIPVFTPDMVLEGGSIDVDGRGTLI
                     TTEQCLLHPNRNPSLSREGIEQVLMDFLGVSRVIWLKGGIAGDDTDGHVDDVARFVDE
                     RTVVCALEKERGSDNYKILKENYEILRRT"
BASE COUNT          332 a          515 c          435 g          334 t
ORIGIN      
        1 ggtcctgcgc agaatctcgt aattctcttt gagtattttg tagttatcgc tcccgcgctc
       61 cttctcaagg gcacacacga cagtcctttc gtccacgaac ctggccacgt catcgacatg
      121 gccatcggtg tcgtcgcctg caattccccc cttcagccag atgacacggg agactccaag
      181 gaagtccatg agcacctgct cgatcccctc cctcgagagt gaagggttac ggttggggtg
      241 gaggaggcat tgctcagtgg tgatcagggt gccgcgaccg tcgacatcga tagatcctcc
      301 ttcaagaacc atgtcggggg tgaatacagg aatatgcaat gacctggcaa ggactgaagg
      361 gatgatgtca tccccctcga gctccgggta ttttccaccc cacgcattga atctccagtt
      421 caccatagcc atcgaagaat cgcgcgcatg cacgaggaac gtggggccgt aatccctgaa
      481 ccacacgtcg cagtaagggt aaacgaagaa cttcacatgc catggttcga tgccggcaca
      541 agagaggatc tcctccaccc tggatcgcat ctcctggtct ctgaccatca ggtataccct
      601 ttctctcccc cggagtgcct ggatcatccc taagtaggag tgctcgacgc gtgggagatc
      661 gaagaacgtg tcgttatcat gcggccatga gagccagatc ccctggtggg gctcccattc
      721 cgcaggcatg cgaaaaccag agggcgctcg cgcctccgct ccaggaaatg tgccggtgtc
      781 caccagaggg ccataggtgt caggacgccg gttactgaag aatccccatc cttcccggac
      841 gaggtcgttt tgcgagaggt ctatctctgc gacaataatc tcctcccccc tgcctgcctt
      901 tgcgataata ttcccgaacg catcgcagac aaaggacccg ccaaagaacg tgatctcccc
      961 ttcaatcccc accctgttca ctgccgccag gtggaccccg ttggcaatcg cgtgacctct
     1021 ctgtaccgtc tgccaggcat cctcccagtc tccctccagg gggtcttcca ttccatggac
     1081 ggtgccaatc gcggtggggt aaaagatcac ctgcgccccc atcagcgaaa ccgtcctcgc
     1141 cgcttcgggg aaccactggt cgtagcatat gaggaccgcg atacggccaa aatgggtctc
     1201 ttccacgacg taggaggacc ctggtgagaa atactccttc tcgtagtaag acggatcgtg
     1261 cggtatgtgc actttccggt atacatcccc agtcgaaccg tcagaccgga tgatgactgc
     1321 tgcgttgtag cggcggccac ccgcgccctt ctcgaggaga ggtgcgacga ttagcccctg
     1381 gttccgccgc gcaatctctc caagcaccct cgttgtctcc ccggggatat gttccgcgta
     1441 ccgatccgcg tcggcaccat ggaccctcgg gaaatagggt gcggagaaga gctcctgcag
     1501 gcagacgacc tgcgctccgc ggtcgaacgc gtcctgtacc atccctgaaa ccttctcaag
     1561 gttccctgcg tggtcaggcc cggcggaagc ctggaccagc ccgatccgta tgcgcg
//
LOCUS       DAOQVJ010000424         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_103539_length_1610_cov_5.301608, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000424 DAOQVJ010000000
VERSION     DAOQVJ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103539_length_1610_cov_5.301608"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PKL26_08120"
     CDS             complement(<1..1599)
                     /locus_tag="PKL26_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNS83699.1"
                     /translation="MRRTVVYTILILLLAIGTAIFAGMQFVMIPAIEQIESREASESV
                     LRTVGMVENEIDSLEGTAVDWSQWDDTYFFAMDRNGEYIKNNLMDKTFTSLQLNLMLY
                     YDLNGTLFYGKGYDYLEFERLEIPQALAVNPAGDILDEIRRNERVEDASIRGIISLPE
                     GLLLLSINPILKSDSTGPAAGYLCIGRYLGDAEVAKIAQLTSTDLSVISAADAGTAWG
                     VMHETLSTQEGSAIVRISPEGNTITSFVVIDDIHGVPAAVFRVDSPRTTHQIAMESLL
                     LSLYLIAAIGCISVLFVIYILDHRFLKRLSLLEKKMKEIADRQDFSARTRLSGDDELS
                     SLSAKIDHTLDALEEHINAERAAVESARIANEKLTLLSKITRHDILNQVTVIRGFADL
                     TRDALAADSPANPFIDRISSASAAIEEQLAFTREYELGGDHAKPAWFNIRDLATGIVR
                     TMPLCEIDVDIDTGDLEIYSDPLLERVIFNLVDNSLGHGEKVTTIRLRFYEEEGSGVL
                     VYEDNGVGIPYHQKEIIFEQGFGRH"
BASE COUNT          363 a          457 c          413 g          377 t
ORIGIN      
        1 atgcctcccg aacccctgct cgaagattat ttccttctgg tgatatggga ttcccacacc
       61 gttgtcctcg tagacaagga cgcctgaacc ttcctcctca tagaacctga gccggattgt
      121 ggtcaccttc tctccatgac caagtgaatt gtccaccagg ttgaatatca ccctctcgag
      181 gagcggatcg gaatatatct cgagatcccc ggtatcgatg tccacatcga tttcacaaag
      241 aggcatggtc cttacaatgc cggtggcgag atcacggatg ttgaaccacg ccggcttcgc
      301 atggtccccg cctaactcgt actcccgggt gaatgccagc tgctcttcga tagccgcaga
      361 cgcggaactg atccggtcga taaatgggtt cgctggcgag tcggcagcca gggcatcacg
      421 ggttaggtct gcaaaacccc tgatcaccgt gacctggttt aaaatgtcat ggcgcgtgat
      481 cttcgagagc agtgtcagtt tctcattcgc aatcctcgca ctctccaccg cagcgcgttc
      541 tgcgttgatg tgctcctcaa gtgcatcaag agtatggtca atcttcgcag agaggctgga
      601 gagttcatcg tcgccggaga gccgggtgcg ggctgaaaaa tcctgcctgt ccgcgatctc
      661 cttcatcttc ttttcaagaa gggagagcct tttcaggaac ctgtggtcca ggatataaat
      721 gacgaaaagg acgctgatgc acccgattgc ggcgatgagg tacagggaaa ggagcaggct
      781 ctccatcgca atctggtggg tagtcctcgg actgtcgacc cggaatactg ctgcgggcac
      841 cccatggata tcatcaatca cgacgaacga agtgatggta tttccctccg gagagatcct
      901 cacgatagca gacccctcct gtgtggaaag ggtctcatgc ataacccccc acgcagtccc
      961 cgcatccgct gcgctgatga ccgagaggtc agtcgaggtc agttgtgcaa tctttgcaac
     1021 ctccgcgtca ccaaggtacc gcccgatgca gaggtagcct gccgccggcc ccgtagaatc
     1081 gctcttcaat attgggttga ttgagaggag gagcagccct tcaggaagac tgatgatccc
     1141 tctgatactg gcatcctcca ctctttcgtt cctgcgaatc tcgtcgagaa tgtcccctgc
     1201 agggttgacc gcaagtgcct gcggaatttc gagacgctcg aattcgaggt aatcatagcc
     1261 tttcccgtaa aaaagggtcc cgttgaggtc ataatacagc atgaggttga gctgcaggct
     1321 tgtaaacgtc ttatccatca ggttgttctt tatgtattct ccgttccgat ccattgcaaa
     1381 aaagtaagta tcgtcccact ggctccagtc gacggcggtc ccttcaagag aatcaatctc
     1441 gttctccacc atccctacag tccggagcac gctctcggat gcctcccttg actcaatctg
     1501 ctcgatggca gggatcatca caaactgcat acccgcaaag attgccgtgc caatggcgag
     1561 taagagaatg agaatggtgt agacaactgt cctgcgcacc tccatgagtc
//
LOCUS       DAOQVJ010000425         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_105069_length_1596_cov_3.304348, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000425 DAOQVJ010000000
VERSION     DAOQVJ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105069_length_1596_cov_3.304348"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKL26_08125"
     CDS             complement(<1..741)
                     /locus_tag="PKL26_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNS83700.1"
                     /translation="MRLGLIGNPNVGKSLIFHQLTGLGVEVSNYPGTTVDLTRGNLCF
                     QNERLELVDLPGVYSLDGTSPEEEIVRSFILGEEVEVLVAVLDATHLERNLYLLLQVA
                     EYGKPMVAVLNMMDEAAKKGKEIDAGRLASMLGIEVIPAAAIQGKNIAEIIPAALCRA
                     RPPIVQVKYDHHIEAAIRSIQSVKPVTRLQALQALQGLGNDADALELAGALAPEIEEM
                     HRMSVPQIIAGNRHHCAHTISREVILELE"
     gene            complement(738..1367)
                     /locus_tag="PKL26_08130"
     CDS             complement(738..1367)
                     /locus_tag="PKL26_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNS83701.1"
                     /translation="MNTSIREDFLEALCVLSRRSPQLPTVSELAAYLSRGASELTSEL
                     QLMEQEGDIRILPDERVELTEKGSETGSRVMRKHQVLARFLSEVLGMDPDAASEEACI
                     LEHDISDEAFERLDRYIRRPRGRGCRMLQGRARLPSLLQFPEGSVLKVLSVRCPGGCE
                     RLADMGIFPGETVKIVHALNNKTVVVQAKGCDIALSPEIASCIFVEKRE"
BASE COUNT          350 a          428 c          427 g          391 t
ORIGIN      
        1 ctccagttca aggatcactt cccgtgaaat ggtatgtgcg cagtgatgtc ggttccctgc
       61 gatgatctgt ggcaccgaca tgcggtgcat ctcctctatc tcgggggcca gggctcctgc
      121 aagttccagt gcatcggcat cattccctaa cccctgtaag gcctggaggg cctggagacg
      181 ggtgaccggc ttgacggact ggatgcttcg tatggcggcc tcgatatggt ggtcgtattt
      241 gacctgcaca atgggagggc gtgcacggca gagtgcggca gggattatct cagcaatatt
      301 ctttccctgg atagctgctg cagggatcac ttcaattccg agcattgagg cgaggcgccc
      361 ggcatcgatc tcttttcctt tcttggcagc ctcgtccatc atgttcagga cggcaaccat
      421 cggcttgccg tattctgcaa cctggaggag gagatagaga ttccgctcca ggtgggttgc
      481 atcgaggact gcaacgagta cctcaacctc ctctcccagg ataaatgacc tgacaatctc
      541 ctcttcggga gaggtcccat cgagtgaata gacgccgggg aggtcgacta gttccaatcg
      601 ttcgttctga aaacagagat tcccccgtgt gagatctacg gtggtgccgg gatagttgct
      661 cacctcgacc ccgagtccag tgagctggtg aaagatgagg gattttccaa cattggggtt
      721 tccaatgagt cccagcctca ttcccgcttc tccacgaata tgcaggaggc gatctcggga
      781 gagagggcga tatcgcatcc ttttgcctgg actacgactg tcttgttgtt cagtgcatgg
      841 acaatcttga ctgtttctcc cgggaaaatg cccatgtcag cgaggcgttc acaccccccg
      901 ggacatctga cagagagaac cttcagcacg ctgccttccg gaaactgaag gagcgaggga
      961 agccttgccc tgccctggag catcctgcac ccccgtcctc tcggtctcct gatatacctg
     1021 tcaagccgct cgaacgcttc atccgagatg tcatgttcga ggatgcacgc ctcttcagat
     1081 gccgcatcag ggtccatccc aaggacttca gagaggaaac gcgcgagaac ctggtgtttc
     1141 ctcattacac ggctaccggt ctctgatccc ttctcggtca gttcaacccg ctcgtccggc
     1201 aggatacgga tatccccctc ctgctccatg agctgcagtt cactggtgag ttccgacgct
     1261 ccccgcgaaa gataggctgc cagttctgac acggtgggaa gttgtggaga tctccgggat
     1321 aacacgcaga gtgcttcaag aaaatcctcc cggatcgagg tattcatgct caacatgttg
     1381 gagatggcat tatttgagaa tttgtatgag gtcagacacg agatgtgcga agcgtaccaa
     1441 atcgcaccga tcccactttt ttaattcgtg gtcccatgca tcgtgttcac cgttattgaa
     1501 tattgaacat tattgatact caaatgtaat agatggtaac aatcatttaa tatattactt
     1561 cggagacagt atcatgaaat gaggtggcat ggtgaa
//
LOCUS       DAOQVJ010000426         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_105284_length_1594_cov_3.346979, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000426 DAOQVJ010000000
VERSION     DAOQVJ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105284_length_1594_cov_3.346979"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKL26_08135"
     CDS             complement(<1..292)
                     /locus_tag="PKL26_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83702.1"
                     /translation="MENWIITLPPDASILHGQNITDNPNLKAKDKYIENSPRLPFMRD
                     ATLQYIHLILVTSLIAGMIVSSGCTNTEEKPGRLKGFDEFVTAKMAEYEVPGA"
     gene            complement(424..496)
                     /locus_tag="PKL26_08140"
     tRNA            complement(424..496)
                     /locus_tag="PKL26_08140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:His,seq:gtg)
     gene            713..>1594
                     /gene="infB"
                     /locus_tag="PKL26_08145"
     CDS             713..>1594
                     /gene="infB"
                     /locus_tag="PKL26_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNS83703.1"
                     /translation="MTQKTHIRTPIVCVLGHVDHGKTSLLDRIRGSSVVSTEDGAITQ
                     HIGATIVPIDAIRKMSGSMEKLALNVPGLLFIDTPGHHAFTTLRARGGALADMAILVV
                     DINQGFQPQTIEALQILRTYKTPFVIAATKIDRLHGWRVHRDEGFLASFSKQNDRVRE
                     LVETRVYEIVGELSDLGFSGERFDRVSDFRRNLAIVPVSAHTGEGVAELLMVMIGLAQ
                     RYMEQDLSLSVEGPGMGTILEVKEEKGLGTTLDVILYDGKLAVGDELAVARQDGLVVT
                     KVRALLQPRPLKEILVED"
BASE COUNT          352 a          421 c          456 g          365 t
ORIGIN      
        1 ccgcaccagg tacttcgtat tcggccatct tcgcggtaac aaactcatca aaacctttga
       61 gcctgccggg cttttcttca gtattggtgc agcctgaaga gacaatcatg ccagcaataa
      121 gtgaggtgac aaggatgagg tgtatgtatt gcaaggtggc atctctcatg aaagggagtc
      181 gggggctgtt ctcaatatat ttgtcctttg cttttaggtt tggattgtcc gtgatattct
      241 ggccgtgaag aatagaggca tcagggggta gagtgatgat ccagttctcc aacaagttct
      301 cctgttactg gagtgctttt attaaagaac ccgacagcca gctgtgtgca tttttcaaga
      361 attactctaa taagtggtaa attgagcctg gaatcaagaa tggaccctca ttagaggtga
      421 ttaggccagg gccgagactc gaacccgggt cgggggatcc acagtcccct aggataacca
      481 actaccccac cctggcagga tgtaccctcc tttatttcac cccggcactc tttaatatat
      541 ctcctcctca caaccctcac acccaaaaga tcgccggtgt gtatttaatt cctggtgata
      601 cattacattg cacagttgat gcctccggat tcccgccatg gctttctgtt tcggggcttt
      661 gcctgcaggc cctgccgata tccggtgtga attacccctg gggtggagga gaatgacaca
      721 gaagacgcat atcaggacgc ccattgtctg cgtgctcggt cacgtggatc acggcaaaac
      781 atcgctcctt gaccggataa gggggtcatc tgtcgtgagc acggaggatg gcgcgatcac
      841 gcagcatatc ggggcaacca tcgttcccat cgacgccatc aggaagatga gcgggagcat
      901 ggagaagctc gcgctgaatg tcccgggcct cctcttcatc gatacccccg gtcaccatgc
      961 gttcaccacc ctgcgggcaa ggggaggtgc actggcggac atggcgattc ttgtggtgga
     1021 tatcaaccag ggtttccagc cccagacgat cgaggcactc cagatcctcc gcacctacaa
     1081 gaccccgttt gtcattgccg caacgaaaat tgaccgcctt cacggctgga gggttcacag
     1141 ggacgagggt ttcctggcga gtttctcaaa gcagaatgac agggtcaggg aactggtgga
     1201 gacccgcgtg tacgagattg ttggcgagct ctcggatctc gggttctcgg gggaacggtt
     1261 cgaccgtgtc tcggacttcc gccggaacct cgccattgtc cctgtcagcg cccatacggg
     1321 ggagggagtg gcagagctcc tgatggtgat gatagggctt gcgcagcggt acatggagca
     1381 ggacctctcg ctctcggtgg aaggccctgg catggggacc attcttgaag taaaagagga
     1441 gaaagggctt gggaccaccc tcgatgtcat cctatatgac ggaaagctcg cggtcggcga
     1501 cgagctcgcg gttgcacggc aggatggcct cgtggtcaca aaggtccgtg cccttctgca
     1561 gccccgcccc ttgaaggaga tacttgtaga ggac
//
LOCUS       DAOQVJ010000427         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_106624_length_1582_cov_4.833006, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000427 DAOQVJ010000000
VERSION     DAOQVJ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106624_length_1582_cov_4.833006"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1498
                     /locus_tag="PKL26_08150"
     CDS             326..1498
                     /locus_tag="PKL26_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNS83704.1"
                     /translation="MSTPRCVTEIPRITCSLFGAIKAAATLKNTVILVHGPKGCVYHV
                     NYILGLRGDRHCPVYCTCMDEHDVVFGAEERLREAIEDLDARKNPEILVVLSCCASGI
                     IGEDVQNACRVARTRSKVVGIDAGGFADDFTGGYARTLHAIVSELAGPAERQDPRRVN
                     LLGMLRAGPDLHEIRRLLSLLGLFTGAVIPAGASYDEIRCIGAASLNIVVCETAGTEA
                     ARYLQEKFKTPYIRTVYPIGAELSRRFLVDIASALGISFLPALVDDPAGNHDTSRRIR
                     IALFSGPTRALALGRFLSSHGLSPAIIILDFENPFTDEIRGAAGNECTVLVSPGWDEI
                     EDALNAHRVDLVLGGLMERPIAARLGIPLIDVMHGSLMTAGPEGGAAILRLIREHT"
BASE COUNT          330 a          464 c          437 g          351 t
ORIGIN      
        1 ccctgctctc catttctttt gctttccgaa aaataaggct caaatgtccc tgacatgacc
       61 ttccttgcat gggtgcgaac gatgaaaacg accggccgaa ctgccatgca ttccgttacc
      121 cggactgtaa tgctctcctg cgtgggaaga cgccgaatcc cttacgcccc cggaaagaag
      181 gtgcgggaga ggttggaaac catgcaaggg aaaagcgcca tttcgtcaaa gaggcttcct
      241 ggtattgttc atgtctatga aaccatcccg tcacaccctc aaccgataag ctataacatc
      301 gatccctgac ccatagactt ctgttgtgtc cactccccgg tgtgtcactg agataccccg
      361 catcacctgc agcctcttcg gtgcaatcaa ggccgcggcc accctgaaaa ataccgtaat
      421 cctcgtgcat ggaccaaaag gttgcgtcta tcatgtcaac tatatcctgg ggctccgcgg
      481 tgaccgccat tgccccgtct actgtacctg catggacgaa catgacgtgg tcttcggcgc
      541 agaggagcgg ttgagagagg cgatcgagga tcttgatgca cgtaagaatc cggagatact
      601 ggtggtcctc tcctgctgtg catcggggat aatcggggag gatgtccaga acgcctgcag
      661 ggtcgcccgc acccgttcga aagttgtcgg catcgatgcg ggagggtttg ctgatgattt
      721 taccggagga tatgcgagga cactgcacgc aatcgtctct gaacttgccg gacctgctga
      781 acggcaggac ccgcggaggg tcaacctcct ggggatgctc agggccgggc ccgacctgca
      841 cgagattcgc cggctcctct cgctgctcgg tctttttaca ggcgcggtta tccctgctgg
      901 tgcatcatat gacgaaatac gctgcattgg cgctgcatct ctgaatatcg tggtatgcga
      961 gactgcaggc accgaggctg cccgttacct ccaggagaag tttaaaaccc cgtatatacg
     1021 gacagtgtat cccatcggcg ccgaactgag tcgcagattt ctcgtggaca tcgcttctgc
     1081 cctcggtatc tccttcctcc ctgctctcgt tgatgatccc gcaggcaatc atgatacgtc
     1141 gcgaaggatc aggatagcac tcttctccgg ccctaccagg gctctcgccc ttggccgttt
     1201 cctctcctca catggcctgt cgcccgcgat aatcatcctc gatttcgaga acccgttcac
     1261 cgatgagatc aggggtgcag caggaaatga gtgcacggtt ctggtctctc ctgggtggga
     1321 tgagattgaa gatgccttga acgctcaccg ggtcgacctc gtgctcggcg gactgatgga
     1381 gcgacccatc gccgcgagac ttggaattcc cctgatcgac gtgatgcacg ggagcctaat
     1441 gactgccggc ccggaagggg gggccgccat cctccgcctg atcagggagc atacctgaac
     1501 gggcgcattc caccgcattc acttcaattc aatcacgcga tgggaagcct gcaggaacgg
     1561 gatggatggg aagcgattgc ct
//
LOCUS       DAOQVJ010000428         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_107099_length_1578_cov_3.846356, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000428 DAOQVJ010000000
VERSION     DAOQVJ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107099_length_1578_cov_3.846356"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PKL26_08155"
     CDS             complement(<1..1009)
                     /locus_tag="PKL26_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83705.1"
                     /translation="MTFELKQSLKLSQQLIMTPQLQQAIKLLQLSRIELIDMIQQEME
                     ENPLLEERASEEFGEEGAPAPEVDEVKTAERTGEVTGEGDGKEDFDWDSYLEDYGPMR
                     VTYDREEDEGPSWENMLSRKTTLTDHLMWQLGLSRLSEAERRIGEQIVGNLDTSGYLA
                     ATVEEIAEQETVGVEVVEKVLARIQEFDPPGIAARDLRECLLLQARYVGGSHALVETI
                     IRDYLHDLETKNYNNIAKKLKIPLADVLDAIFLISRMDPKPGLVYSDEGPQYIIPDVF
                     VFKVGDEYKIILNDEGLPRLRISNFYREILGGGSEGAQSGDECKKYIKERLQSATWLI
                     KS"
     gene            complement(1031..>1578)
                     /gene="lptB"
                     /locus_tag="PKL26_08160"
     CDS             complement(1031..>1578)
                     /gene="lptB"
                     /locus_tag="PKL26_08160"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNS83706.1"
                     /translation="IFLDAEEITRDPMYVRARKGINYLPQEPSVFRKLTVKENILAIL
                     ETLDIDREERKNRLQQLLGELDLTGLASSKAYSLSGGERRRVEITRALVTSPRFILLD
                     EPFAGIDPLAVADIQKIIQKLKAKGIGVVISDHNVRETLSVCDRAYILNEGTVLIEGL
                     PDMIAESKIARKFYLGDDFRF"
BASE COUNT          240 a          524 c          467 g          347 t
ORIGIN      
        1 tgctcttgat gagccacgtg gccgactgga gcctctcctt gatgtatttc ttgcactcgt
       61 cgccgctctg cgccccttcc gaaccgcccc ccaggatctc gcggtagaag ttgctgatcc
      121 gcagccgggg gagaccctcg tcgttcagga tgatcttgta ctcgtcgccc accttgaaaa
      181 cgaacacgtc ggggatgatg tactgggggc cctcgtcgct gtacacgaga cccggcttcg
      241 ggtccatgcg gctgatgagg aagatggcgt ccaggacgtc cgccaggggg attttcagtt
      301 tcttcgcgat gttgttgtag ttcttcgtct ccaggtcgtg gaggtagtcg cggatgatcg
      361 tctcgaccag ggcgtgggag ccgcccacgt agcgggcctg cagcagcagg cactcccgga
      421 ggtcccgcgc cgcgatcccc ggcgggtcga attcctggat ccgggccagc accttctcga
      481 ccacctcgac gccgactgtc tcctgctccg cgatctcctc gacggtggcc gcgaggtagc
      541 cgctcgtgtc caggttgccg acgatctgct cgccgatccg gcgttccgct tccgagaggc
      601 gggacagtcc cagctgccac atcaggtggt ccgtgagcgt cgtcttgcgg gacagcatgt
      661 tctcccagga aggcccctcg tcctcctccc ggtcgtacgt cacccgcatg gggccgtaat
      721 cttccaggta gctgtcccag tcgaaatctt cctttccgtc cccctcgccc gtcacctcgc
      781 cggtccgctc cgccgtcttg acctcgtcga cctccggagc gggcgcccct tcctcgccga
      841 actcttcgct cgcgcgctcc tcgagcaggg ggttctcctc catctcctgc tggatcatgt
      901 cgatcagctc gatccgggac agctggagca gcttgatcgc ctgctgcagc tggggcgtca
      961 tgatcagctg ctggctgagc tttaggcttt gcttgagttc gaacgtcatg ccttcctgcc
     1021 tgctgaggtc ctaaaacctg aagtcatcgc ccaggtagaa cttccgggcg attttactct
     1081 ccgcgatcat gtccggcaag ccctcgatca gcaccgtccc ctcgttgagg atgtaggccc
     1141 ggtcgcacac cgaaagggtc tcccgcacgt tgtggtccga gatgaccacc ccgatgccct
     1201 tggccttgag cttctggatg atcttctgga tatctgccac ggccagggga tcgatccccg
     1261 caaacggctc gtcgagcagg atgaaccgcg gggacgtgac cagcgcccgg gtgatctcca
     1321 cgcgccgcct ctcgcccccc gagagggagt aggccttgct cgaggccagg ccggtgagat
     1381 cgagttcgcc gagcaattgc tggagcctat tcttcctctc ctcgcggtcg atgtcaaggg
     1441 tttcgagaat cgccagaata ttttccttga cagtcagctt cctgaatacg gagggctcct
     1501 ggggcaggta gttgatgccc tttcgggccc tcacgtacat ggggtcccgg gtgatctcct
     1561 cggcatcgag gaaaatcc
//
LOCUS       DAOQVJ010000429         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_107220_length_1577_cov_3.625493, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000429 DAOQVJ010000000
VERSION     DAOQVJ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107220_length_1577_cov_3.625493"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKL26_08165"
     CDS             <1..491
                     /locus_tag="PKL26_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS83707.1"
                     /translation="KVYFPRVIIPLTSVLAGLLDFSLAFIVLLGMMLYYHIYPTLLVL
                     VLPGLVILMMLTASGVGMLLAALNAKYRDIRYTIPFLVQFWMFATPIVYPASMIPERY
                     RMIYALNPMTGIIEGFRAVLLGRAAFPTEMVLISIVLSLGLFILGLTYFKQVERYFAD
                     VI"
     gene            494..>1577
                     /locus_tag="PKL26_08170"
     CDS             494..>1577
                     /locus_tag="PKL26_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HNS83708.1"
                     /translation="MSSPIIQVKGLSKQYHIGLREKTNRTFREAVTDAFTAPVRNFRN
                     LRRLTKVENDNPAVIWALRDVSFDVQPGEVVGIIGRNGAGKSTLLKILSRITEPTSGE
                     IRLHGRVASLLEVGTGFHPELTGRENIYLNGTILGMRKHEVDRKFDEIVAFSEIEKFL
                     DTPVKRYSSGMYVRLAFAVAAHLEPEILLVDEVLAVGDAAFQKKCLGKLKDVSGTGRT
                     TFYVSHNMGSIVDLCTKAILLDKGTLKVSGPPSGIVEEYLSANSNKSFVDLTRWYEER
                     SGNGPLKLSFIETINQSGRPVSQFSYGEPITFRIGIQGQPHSLMNLGISIRNTLNQLV
                     LHFTNKDDHISLEMRSRETTICVRVDQ"
BASE COUNT          369 a          445 c          398 g          365 t
ORIGIN      
        1 ccaaggtcta cttcccccgc gtcatcatcc cccttacctc cgtactggcc gggctcctgg
       61 acttttccct ggccttcatc gtgcttctcg ggatgatgct gtactaccac atctacccaa
      121 ccctcctggt acttgtcctg ccggggctcg tcatactcat gatgcttacg gcgagcggcg
      181 tggggatgct gctcgcagca ctcaacgcga aataccgcga catcaggtac acgatcccct
      241 tcctggtaca gttctggatg ttcgcgactc ccattgtgta tcccgcctcc atgatccctg
      301 aacggtaccg catgatctat gcgctcaatc ccatgaccgg catcatagaa ggctttcggg
      361 cggtcctgct gggaagggct gcatttccaa cggaaatggt cttgatctct atagtcctca
      421 gtttgggact ttttattctc ggcctaacct acttcaaaca ggtcgagcga tacttcgccg
      481 acgttatctg atcatgagca gccccatcat tcaggtcaaa ggcctttcca agcaatacca
      541 tatcggcctc cgtgagaaga caaaccgtac cttccgcgag gccgtcaccg atgcctttac
      601 cgcccctgtc cggaatttca ggaaccttcg acgcctcacc aaggtcgaaa acgataaccc
      661 cgctgtcata tgggcgctca gggacgtctc attcgatgtc cagcccggcg aggtcgttgg
      721 tatcataggt aggaacggcg caggcaagag caccctgctc aagatactat cccgcatcac
      781 cgagccgaca tctggtgaga taaggctcca cggcagggtg gcgagccttc ttgaagtcgg
      841 gacagggttc catccagagc tcaccggccg cgaaaacatc tacctgaacg gcaccatcct
      901 cggcatgagg aagcatgagg tcgatcgtaa gttcgacgag atcgtggcct tcagcgagat
      961 cgagaagttc ctcgatacac cggtgaagag atactccagc ggcatgtatg tgcggctcgc
     1021 ttttgccgtg gctgcacacc tggaacccga aatcctcctg gtggatgaag tgttagcggt
     1081 tggtgatgcg gctttccaga aaaaatgcct ggggaagtta aaggatgtat cggggaccgg
     1141 gagaaccacg ttctatgtaa gtcacaatat gggttcaata gtcgatctgt gcacaaaggc
     1201 cattctcctt gataaaggca ccttaaaggt atccggccca ccttcaggca tcgtagaaga
     1261 gtatctgtcg gcaaacagca ataagagttt cgttgacttg acacgctggt acgaggagag
     1321 gtcagggaat ggtcccctga agctttcttt catcgagaca atcaatcagt ccggcagacc
     1381 ggtatcccaa ttttcatatg gagagccaat aactttcagg atcggcattc aaggccaacc
     1441 tcattccctc atgaatcttg gtatctccat aagaaatacg ctcaatcagc tggtactgca
     1501 ctttacaaac aaggacgatc acatttcgct ggaaatgagg tcgagggaga cgaccatctg
     1561 tgtccgtgtg gaccaga
//
LOCUS       DAOQVJ010000430         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_108335_length_1567_cov_4.128307, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000430 DAOQVJ010000000
VERSION     DAOQVJ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108335_length_1567_cov_4.128307"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PKL26_08175"
     CDS             <1..808
                     /locus_tag="PKL26_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83709.1"
                     /translation="RQQAICTWIDTAPVLPFEPWIFPSAESLRGICDEVSDPWVCLYC
                     MTSATELRLDVRSVLTINAFLTAESDFEAFLSDCKKPGETDATSFVRSSYNITTSIRA
                     SASHFTPVDVLWMDWLSEREDIRALSTGHDRSVSYHIYPATLQGIYESAGSKEIYYRL
                     PSKKVRNLLAIAGGDERRYFTKSGRLQGFGSKVGEPWRNPQELVFVRSEDLHKALNSQ
                     NLKIFWTVNLLREPSPCAREEFPDLYHIQDRYWVFWEGKGGLQSVEILLE"
     gene            833..1015
                     /locus_tag="PKL26_08180"
     CDS             833..1015
                     /locus_tag="PKL26_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83710.1"
                     /translation="MTPDWTGFIGSNLADTLASLVLDIPSCGRSHLAQNHDVVISDNL
                     ATGRREKSNSSSTTVG"
     gene            1266..>1567
                     /gene="cdc6"
                     /locus_tag="PKL26_08185"
     CDS             1266..>1567
                     /gene="cdc6"
                     /locus_tag="PKL26_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867134.1"
                     /note="Involved in the initiation of DNA replication;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division control protein 6"
                     /protein_id="HNS83711.1"
                     /translation="MRTRLLMSDQTLFRSIDVFEIDYAPEFFNYRESQLKDLAYQIRP
                     ALGGGRALNAICRGLPGTGKTTSVLRIFTELEHTTKKIVPVYVNCQNDRTKYMVFSR"
BASE COUNT          352 a          466 c          417 g          332 t
ORIGIN      
        1 gcggcagcaa gctatctgca cctggataga tacggccccg gtcctaccct ttgaaccctg
       61 gatctttccg tcagccgaga gcctccgtgg gatctgcgat gaagtctccg atccatgggt
      121 ctgcctctac tgcatgacct ccgccaccga actccggctc gatgtgcgat cggttctcac
      181 catcaacgca ttcttgactg cagagagcga ctttgaagcc ttcctctcgg actgcaaaaa
      241 gccaggggag acagacgcga catccttcgt ccgatccagt tacaatatca caacatctat
      301 ccgggcatcg gcatcccact tcactccggt agatgtgctc tggatggact ggctttctga
      361 acgggaggat atacgtgcgt tatctacagg tcatgaccgc tcggtgtcat accacattta
      421 cccggcaacc ctgcagggga tctacgaatc ggcaggctcc aaagagattt actacaggct
      481 tccctcgaag aaggtgcgca atctgctagc gattgccggg ggagatgaaa gacggtattt
      541 caccaaatcc gggcggcttc agggcttcgg atcaaaagtt ggagaaccct ggaggaatcc
      601 acaggaactc gtcttcgtca ggagcgagga tcttcataaa gctctgaata gtcagaacct
      661 caagatattt tggaccgtca acctgttgcg ggaaccctct ccctgcgccc gggaagaatt
      721 tcctgatctt taccatattc aggatcgcta ctgggtattc tgggaaggaa aaggggggct
      781 gcagtcggtt gagatcctgc ttgagtgaga gaatctgcag atttccgcca ggatgactcc
      841 agactggacc gggttcatcg gctccaacct tgcagacacc ctggcatctc tggtgctcga
      901 tatcccatcc tgcggccggt cgcacctagc acaaaaccac gatgtcgtca tcagcgacaa
      961 cctcgccacc gggcggcgag aaaaatcgaa ctcctcctcg accaccgtcg ggtgacgttc
     1021 atcggggagt atcacgaacc tcgaccctcc tcatgaaggt gcgatgggcg ggggccagcg
     1081 aggtggtggc agtgctggtg aaggtgctcg gcggatgatc cgccatccct gattttcggc
     1141 cgtagtgccc tctagtggac aaagcaacgg tgataagagc gtaaaatcct cccggtgcaa
     1201 ctcctttttc actttcacca tgcgcgcggt ccccgtttaa ataaccgcct cccgacgatc
     1261 cttatatgag aacgcgactc ctcatgtccg accagacact cttccggagc atcgatgtct
     1321 tcgagatcga ttacgctcca gagttcttca actaccgcga aagccagctc aaggaccttg
     1381 cctaccagat ccgccccgca ctcggaggcg ggcgggcgct gaacgccatc tgcaggggtc
     1441 ttcccgggac cgggaagacg accagcgtgc tcaggatctt taccgagctc gagcacacca
     1501 cgaagaagat cgtgccggtc tacgtgaact gccagaacga ccggacgaag tacatggtct
     1561 tctcccg
//
LOCUS       DAOQVJ010000431         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_108341_length_1567_cov_3.662037, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000431 DAOQVJ010000000
VERSION     DAOQVJ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108341_length_1567_cov_3.662037"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..846
                     /locus_tag="PKL26_08190"
     CDS             316..846
                     /locus_tag="PKL26_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNS83712.1"
                     /translation="MDVEHMNLSRFDEMEKGELVSYLGFLLWHYRVVDAFWFIFLAEE
                     YGQPVAERINEQVWGRVASMAARDLKARFGITDTGLKGFVQALRLFPWAILVGYQIEE
                     NADEVVIRVPSCPTQEARLRRGLGEYVCREMHRAEFTAFAHEIDPRIRVECLFAPPDP
                     HPPDMFCMWRFTLSNE"
     gene            complement(851..1189)
                     /locus_tag="PKL26_08195"
     CDS             complement(851..1189)
                     /locus_tag="PKL26_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83713.1"
                     /translation="MTTTPRARPVEFEARSVLDSLGYHALRFNGSGSPVNLVGIDDHE
                     VLLVQVRRERLPYTGIREVNAKYRADMDRLRAIGGPRCCTKEIWVCSGHEGFRFYEVF
                     PGGLMEIERP"
BASE COUNT          281 a          440 c          536 g          310 t
ORIGIN      
        1 ctctgcacga tcccggcaaa ggtggttaca tcagcgatcg gtgcggccag cacgcgggcc
       61 gcggtcttgg tggtgctctt ctgcacaaag tctgccattc tcttctttcc tgcccccctt
      121 gagaggggca catacccggt gggagggggg ttatcaagga gattgggcgc agggggtaaa
      181 ttgagaccag gatagcgtct ttttgccaat agatggcttc tgggttgcag attgaacgat
      241 cggtggcaat tcgcggagaa tattccccgg agaccaaaaa gggaaatgtg atgtggtccc
      301 ggggcgatct ggggcatgga tgttgagcat atgaacctgt cacggttcga tgagatggag
      361 aagggagagc tggtctcgta cctcgggttc ctgctctggc actaccgggt ggtggacgcg
      421 ttctggttca tcttccttgc agaggagtat gggcagccgg tcgctgagcg gatcaacgag
      481 caggtgtggg ggagggtcgc ctcgatggcg gcaagagacc tgaaggcgcg gttcgggatc
      541 actgatacag ggctgaaggg gttcgtacaa gccctccgcc tcttcccatg ggcaatcctc
      601 gtcggctacc agatcgagga gaacgcggac gaggtggtca tcagggtgcc ctcctgcccc
      661 acccaggaag cgcgcctgcg ccgcgggctc ggcgagtacg tctgccggga gatgcacagg
      721 gcggagttca ctgcgtttgc acacgagata gacccgcgga tcagggtgga gtgcctcttc
      781 gctccgcccg acccgcaccc tcccgacatg ttctgcatgt ggcgcttcac tctctcaaat
      841 gaatgagggt tcagggccgc tcgatttcca tcaggcctcc ggggaagacc tcgtagaacc
      901 ggaacccctc atggcccgag cagacccaga tctccttcgt gcagcaccgc ggaccgccta
      961 tcgcacgcag ccggtccatg tcggcccggt attttgcatt cacctcccgg atccccgtgt
     1021 aaggcaggcg ttccctccgg acctggacga ggaggacttc gtggtcgtcg atccccacaa
     1081 ggttcacggg gctgcctgac ccgttgaacc gcagcgcgtg gtatcccagg gagtcgagga
     1141 cggaccgcgc ctcgaactcc acggggcggg ccctcggggt ggtggtcatg gtgcggttcc
     1201 ccccatggcg tggcaaatgg tgcgccgggg tgcatgcgca ggtcgtgcga tgcggcaggt
     1261 gctgcctttc agatgggtat gcgagagtgg attgtcgggc tgggtgagag gggacatgaa
     1321 cgggatcccc ctttttcggg tatgcaggtg tgaatgcagt gcagagtgca tgaccccggg
     1381 attttcctgg gatttttgcg cgggaagaga agcggcgatc acctatcggc gggggaaatg
     1441 gtgaatggct gaggagaggc ggggcctgac agggggcctg tggcgatgcc gcccgtccct
     1501 tccgcattct ccggccccgc cactgcaacc ccgagaagta tcccgatcgt gacgaccacc
     1561 gcatgga
//
LOCUS       DAOQVJ010000432         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_108513_length_1566_cov_2.766380, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000432 DAOQVJ010000000
VERSION     DAOQVJ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108513_length_1566_cov_2.766380"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKL26_08200"
     CDS             complement(<1..685)
                     /locus_tag="PKL26_08200"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNS83714.1"
                     /translation="MNVVLTGFRGTGKTESGRLLARLLDVPFYDTDALVEEEAGATVH
                     EIFEQEGEEGFRAREKRAIAGLPRGPGVFATGGGAVIDPENVEHLRRGRTVVLLNADE
                     STIEKRIQHSTRPALTRMGLRAEIHALMESRRDVYLAAADYCIDTSARSANEVAIGIK
                     RLLAEGVTKESGRREALRAFAGSGIHQSEAAELATMVKALSENPVLRFYGIAGYPSLH
                     SRSPPLFRRL"
     gene            complement(682..>1566)
                     /locus_tag="PKL26_08205"
     CDS             complement(682..>1566)
                     /locus_tag="PKL26_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNS83715.1"
                     /translation="ALTALGGSVSFLEKEGFPPLDVSGSLRGGTARIDAGMSSQFVSS
                     ILMAAPCAMQDVTLELEPGVASPSYLDVTTAVMEAFGARVEREEYRRFHVPAARSYLG
                     RTYQVEGDYSSASYFFAIAALCGGKVSVSNLNPSSCQGDRRFLSALESMGCRVRSTED
                     TVEVSCDNDLDGIEIDMSSSPDTVQTLCMVAANAKSPTRITGIAHLKWKESDRLETTT
                     GLLRSLGGDARVEEDAILIRPAPLHGGMIDPGDDHRTAMSFAVLGLATGNVKITHAEC
                     VSKSFPGFWDQLGGQGLV"
BASE COUNT          271 a          530 c          456 g          309 t
ORIGIN      
        1 agaggcgccg gaagagcggg gggctcctgc tgtggaggct tgggtatccc gcgatgccgt
       61 agaaccgaag gacaggattc tcggagagtg ccttgaccat ggttgccagt tccgcggcct
      121 cggactggtg gatgccggaa cccgcaaacg cgcggagcgc ttccctgcgc ccactttctt
      181 tcgtgacacc ctcggcaagg aggcgcttga tcccaatcgc gacctcgttt gcactccgtg
      241 cgctggtgtc gatgcagtag tcagcggcag cgaggtacac gtccctcctg gactccatga
      301 gggcgtggat ctcggcccgc aaccccatcc tcgtgagcgc agggcgtgtg ctgtgttgga
      361 tgcgcttctc gatcgtcgac tcgtctgcgt tcaggaggac gaccgtcctc ccccttctca
      421 ggtgttccac gttctcggga tctatcaccg ccccgccgcc ggtcgcaaac acccccggtc
      481 cgcggggaag ccccgcaatc gcccgcttct ccctggcgcg gaacccttct tcgccttcct
      541 gttcgaagat ctcgtgcacc gtagcccccg cttcctcctc gaccagtgca tcggtatcgt
      601 aaaaggggac atcgagcagg cgggccagca gccggcccga ctctgttttc cccgtccccc
      661 ggaaccctgt cagcacaacg ttcataccag tccctgcccc ccgagctgat cccagaaacc
      721 cgggaacgat ttactgacac attccgcatg cgtaatcttt acgtttcctg tcgcaaggcc
      781 gaggaccgcg aaactcatcg ccgtccggtg atcatcgccc gggtctatca tcccgccgtg
      841 caggggtgca ggccgaatca agattgcgtc ttcttccacc ctcgcatcgc ctccgaggct
      901 gcgcaggagt cccgttgtcg tttccaggcg gtcgctctcc ttccacttca ggtgggcgat
      961 gccggtaatc cgggtcgggc ttttcgcgtt cgctgcgacc atgcagaggg tctggacggt
     1021 gtcgggtgac gaggacatat cgatctcgat cccatcgagg tcattgtcgc acgagacctc
     1081 gactgtgtct tctgtgctcc tcaccctgca ccccatcgac tcaagggcgg agaggaacct
     1141 cctgtctccc tggcacgagg aggggttcag gttcgagacg gagactttcc ccccgcacag
     1201 ggcagcaatt gcaaagaagt acgacgcgga ggagtagtcc ccctcgacct ggtaagtccg
     1261 gcccaggtac gatcgtgcgg ccggaacatg gaaacgccgg tactcttccc tctccacacg
     1321 tgcgccgaac gcctccatca ccgcggtggt cacgtcaaga taagaggggg atgcaacccc
     1381 aggctcaagc tcgagtgtca catcctgcat cgcacatggt gctgccatca ggatcgagga
     1441 gacgaactgg ctgctcatcc cggcatcgat gcgtgccgtc ccgccgcgga ggcttcccga
     1501 tacgtctaac ggcgggaagc cctctttctc caggaagctg acactcccgc caagcgcggt
     1561 gagcgc
//
LOCUS       DAOQVJ010000433         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_108723_length_1564_cov_3.240557, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000433 DAOQVJ010000000
VERSION     DAOQVJ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108723_length_1564_cov_3.240557"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /gene="thiC"
                     /locus_tag="PKL26_08210"
     CDS             <1..1105
                     /gene="thiC"
                     /locus_tag="PKL26_08210"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNS83716.1"
                     /translation="LRTKVNASVGTSSDIVDIDMEVEKTRQAELAGADTLMELSTGGD
                     FVEIRKRVISATSLSVGSVPLYQAFIEAARKDGAVVHMKEDDLFRITAEQAKLGTNFM
                     AIHTGINWETVKRLQNQGRHGGLVSRGGAFMTAWMLHNEKENPLYSEFDYLLEIMKEH
                     EVTLSTGNGMRAGAVHDATDRAQIQELIINTELGDKAHAEGVQVIIEGPGHIPVDEIQ
                     ANVILQKRMSNRKPFYMLGPLVTDIAPGYDDRVAAIGAALSSSYGADFICYVTPAEHL
                     ALPTPDEVYEGVMSSRIAAHVGDMIKLKKRDADLEMGHARRDLDWERQFQIAMNPERA
                     KKIRDERMPADADACTMCGDYCALKIVNRHFSF"
     gene            1273..>1564
                     /locus_tag="PKL26_08215"
     CDS             1273..>1564
                     /locus_tag="PKL26_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS83717.1"
                     /translation="MKPESATILEEFLKYSDTMTNEIEQDAEEMLGVVPFIFRTLKER
                     PDLFVLSALADYLACRPESLDAKTAELVAMTAAAAANAPDCLKVHIAAARKEG"
BASE COUNT          355 a          478 c          453 g          278 t
ORIGIN      
        1 gctgcggacc aaggtgaacg cctctgtcgg gacctcttcg gatatcgttg acatcgacat
       61 ggaggtggag aagacccgcc aggcagagct cgccggggcc gacaccctga tggagctctc
      121 caccggaggg gacttcgtcg agatcaggaa gcgggtaatc tcagcgacca gcctctcggt
      181 cggaagcgtc ccgctctacc aggcattcat cgaggccgcg aggaaggacg gcgcggtcgt
      241 gcacatgaag gaggacgacc tcttccgcat cacggcggag caggcgaagc ttgggaccaa
      301 cttcatggcg attcataccg ggataaactg ggagaccgtc aagcgcctcc agaaccaggg
      361 ccgccacggc gggctggtct cccgcggcgg ggccttcatg accgcatgga tgctccacaa
      421 cgagaaggag aacccccttt actcggagtt cgactacctt ctggagatca tgaaggagca
      481 cgaggtcacc ctctccacgg gcaatgggat gcgggccggt gcggtacatg atgccactga
      541 ccgtgcccaa atccaggaac tcatcatcaa cacggaactc ggggacaagg cacatgccga
      601 gggtgtgcag gtgataatcg aggggccggg gcacatccct gtcgatgaga tacaggcaaa
      661 cgtgatcctg caaaaacgga tgagcaaccg gaaacccttc tacatgctcg ggccgctcgt
      721 gaccgacatc gcaccgggat acgacgaccg ggtggccgcg atcggtgccg ccctctcctc
      781 atcgtacggc gccgacttca tctgttacgt cacccctgcg gagcacctgg cgctccctac
      841 cccagatgag gtctacgagg gagtcatgag ctcccgcatc gcggcccatg tcggcgacat
      901 gatcaagctg aagaagcggg acgcggacct ggagatggga catgcacgtc gtgacctcga
      961 ttgggagcgg cagttccaga tcgccatgaa ccccgagagg gcaaagaaga tccgggacga
     1021 gcggatgcct gccgatgcgg atgcctgcac aatgtgcggc gactactgtg ccctgaagat
     1081 cgtgaaccgg cacttcagct tctaaatcct ttttttccac ccgcttcgtc tgagtccgct
     1141 cctgcagtga aggagtcaag gtctccgagt atcacgaaga gatgattcgc aaacctttgc
     1201 acacccgatc tttccaatcc ccaacaactg gatttccata ccttcatctc tcctcacctc
     1261 gaaccaacag caatgaaacc ggaatctgcg acgatactgg aggagttcct caaatattcc
     1321 gataccatga ccaacgagat cgagcaggac gcagaagaga tgctcggtgt ggttccgttc
     1381 atctttcgca ccctcaagga acgtcccgat ctgttcgtcc tctcggcact tgccgactac
     1441 cttgcctgcc gccctgaaag cctcgacgca aagacggcgg aattggttgc catgacagct
     1501 gcagccgccg ccaatgcacc ggactgcctc aaggtgcata tcgctgcggc acgaaaggaa
     1561 ggag
//
LOCUS       DAOQVJ010000434         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_109529_length_1557_cov_3.970706, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000434 DAOQVJ010000000
VERSION     DAOQVJ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109529_length_1557_cov_3.970706"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKL26_08220"
     CDS             <1..342
                     /locus_tag="PKL26_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83718.1"
                     /translation="YHPAIERVVLVCHGNAAYILYQDILAQETTINTEYEMAQALFSH
                     LSMIEASHGIRIKNREAIARIVAPAIKSETQALSTGISLNNWVAVAGACGEVTVPESV
                     IRTILDSLKSA"
     gene            570..1460
                     /locus_tag="PKL26_08225"
     CDS             570..1460
                     /locus_tag="PKL26_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HNS83719.1"
                     /translation="MPGLLLQTGYFAVMVFIVTSMLAMGLNISICDLISPLRNRRLVI
                     LSLLANFVIVPLFAFVLIYVFPVSRDLAAGLILVSIAAGAPSTPKVAELTEGNIAYAV
                     SLTLLMTILTIILMPILVPYIMEGMGMNPAKVAFNLVIFMLLPILVGMLLRNRAPERA
                     QKVLPLAELVSNASIGVIFLTFGIIFFAHVVDLFGHPGGFEIAAIAVLFTVGALLIGY
                     FMGGPERGHRDILAFSTGFRNITAALVVITASYSDLDNDVFLMVLMVTLFSVIIVSAI
                     VGIIIKKRIDTGKRSRLQPS"
BASE COUNT          338 a          458 c          429 g          332 t
ORIGIN      
        1 tatcaccccg ccatagagag agtggtgctg gtctgccacg ggaatgccgc atatatcctc
       61 tatcaggata tccttgcgca ggagacgaca atcaacaccg agtatgaaat ggcgcaggcg
      121 ctcttctccc acctctcgat gatcgaggcc agccacggaa tccggattaa gaacagggaa
      181 gcaattgcac gcatcgtcgc acccgcgatt aaaagcgaga cccaggcgct ctcgaccggg
      241 atctcgctga acaactgggt cgcagtagcc ggggcatgcg gagaagtaac ggtgcctgag
      301 agtgtgatcc ggacaatcct cgactcactg aagagtgcgt gagggtgcaa tgcatcccca
      361 tatcgaggga tggcatttgt agaggaaaca gccggttccg aaaagatgtg ccaggaaaga
      421 gacaggccgc gcccttgtcg ggaaaactta ccggccctgt gcctggatgg cgagaccggg
      481 acgggaaaga acccctgtta tcgaatgccg gacgggaagg atacctatat gcggaaaccg
      541 ggtgaactca atggcagagg attcacccga tgccaggatt gctgctgcag acaggctatt
      601 tcgcggtgat ggtatttatt gtcaccagca tgctggcgat gggactcaac atctcgattt
      661 gcgacctcat ctcgcccctc cggaaccgga ggctcgtaat cctctccctc ctggcaaact
      721 tcgtgatcgt ccccctcttc gcgttcgtgc tcatctatgt ctttccggtg agcagggatc
      781 tcgcggcagg gctcatcctc gtcagcattg ccgcaggggc tccttctacg ccgaaggtag
      841 cagaactgac cgaaggaaac atcgcctacg cggtctccct gaccctcctg atgaccatcc
      901 tcaccatcat cctgatgccg atcctcgtcc cctacatcat ggaagggatg gggatgaacc
      961 cggcaaaagt ggcattcaac ctcgtcatct tcatgctcct tcccattctt gtcggcatgc
     1021 tgctccgtaa ccgggcgccg gaaagggcgc agaaggtact cccgcttgcc gaactggtct
     1081 cgaatgcctc catcggggtg atattcctga cctttgggat catcttcttt gcacacgtcg
     1141 tggatctctt cgggcatccc gggggtttcg agattgccgc gatcgccgtg ctgttcacgg
     1201 tcggggctct cctgatcggg tacttcatgg gaggccctga gcggggccac agggatatcc
     1261 ttgcgttcag cacagggttt cggaatatca cggcggcact cgtcgtcatc acggcaagct
     1321 acagcgacct tgacaacgac gtgttcctga tggtcctcat ggtcacactc ttcagcgtga
     1381 tcatcgtctc ggccatcgtc gggatcatca taaagaaacg gattgatacc gggaagcgat
     1441 cgcgccttca gccatcctga agtcatccgc accccctgcc ggcctgaacg accggggtct
     1501 tctcaccaga atctagaaat gacaggtaaa cgatctcact gtatatggca cgatccc
//
LOCUS       DAOQVJ010000435         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_110142_length_1552_cov_5.188377, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000435 DAOQVJ010000000
VERSION     DAOQVJ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110142_length_1552_cov_5.188377"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKL26_08230"
     CDS             <1..599
                     /locus_tag="PKL26_08230"
                     /inference="COORDINATES: protein motif:HMM:NF023806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HNS83720.1"
                     /translation="TKVKAYLVSILLVGLALGGMGLGTFAWFTDTAAVDGNAISTGKV
                     DLEVEIQESANGAQTVLTVENLVPSTDTWTTAGIICLKNVGTVPLKWKGYFVKTGGNL
                     PLDDTLKFRIRQVTDGPLETAPKYSTWDDLKNSDGIFLNSSNFGGGGGQMDPGTQYYK
                     VEVRLDENAGNAYAGKSATFRAVFDATQPENSGWSETS"
     gene            634..921
                     /locus_tag="PKL26_08235"
     CDS             634..921
                     /locus_tag="PKL26_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83721.1"
                     /translation="MNEMQVRRSECAASSAVATPPLPFSSCKPAAGATRGACSGGDTA
                     GDTIALRPQGNKGQFSSHAGAAVRVVCSTAGYVVYLVARRSSPTNSRRCER"
     gene            918..1508
                     /locus_tag="PKL26_08240"
     CDS             918..1508
                     /locus_tag="PKL26_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83722.1"
                     /translation="MMRSIVLLVVICCAGMLLLADPVPTGAWFADTEVIEGISIGTGS
                     WSSTDGLVIAADQPLLKPPAGRRAKTVLSGITASNTGSAPLTITGIRVSWDPDDGEAI
                     REAAFTRGNGGKAPRAEPEEAGPESAGGEGFTVFWSGEAASGQTLDGRMLLKPATHSD
                     PARGVLLSFDANMGGKSLMCTLILDDGSEKGVRVQV"
BASE COUNT          317 a          450 c          500 g          285 t
ORIGIN      
        1 aaacaaaagt gaaagcatat cttgtcagta tcctgctcgt cgggcttgcc ctcggcggga
       61 tgggtctggg aacatttgcc tggtttacgg acacggcagc tgtagatggc aacgctattt
      121 cgactggcaa agtcgacctg gaggtagaga tccaggagtc agcgaatggt gcgcaaacag
      181 tgctgacggt cgaaaacctg gttcccagta cggatacctg gaccactgcg ggtattatct
      241 gcctcaagaa cgtggggact gttcccctga agtggaaggg atactttgtc aaaactggtg
      301 ggaacctccc gctggatgac acgctcaagt tccggatcag acaggtcacg gatggcccct
      361 tggaaaccgc tcccaagtac tctacctggg atgaccttaa aaatagcgac ggcattttcc
      421 tgaactcgag caatttcggg ggcgggggag gccagatgga ccctggtacc cagtattaca
      481 aggttgaggt aaggctggat gagaacgcgg ggaatgccta tgcaggaaaa tcggcaacat
      541 tcagggcagt cttcgacgcg acgcagcccg aaaactccgg gtggtcggag acctcctgag
      601 caatccctga taatccatac cacaccagca atcatgaacg agatgcaggt gagacggagt
      661 gagtgcgcag cctcttcagc cgttgccacg cctcctctcc ccttctcttc ctgtaaaccg
      721 gctgcgggcg ccacccgtgg tgcctgttcc ggcggagata ccgccggaga tacgatcgcg
      781 ctcagaccgc aggggaacaa aggccagttc tcttcccacg cgggagcagc ggtgcgtgtc
      841 gtctgttcga ccgcaggcta cgtggtgtat ctcgtggccc gccggtcctc gcccacgaac
      901 tccaggaggt gtgagcgatg atgaggtcta tcgtgcttct ggtagttatc tgttgcgccg
      961 gtatgctcct gctcgccgat cccgtgccga ccggggcatg gtttgcggac acggaggtga
     1021 tcgaggggat cagcatcggc accgggtcgt ggagcagcac cgatggcctg gtgatagccg
     1081 cggatcagcc tctgctcaaa ccaccggcag gccgccgggc aaagaccgtc ctctccggca
     1141 tcaccgcgag caacaccggg tctgctccgc tcacgatcac gggcatccgg gtctcgtggg
     1201 accccgacga cggtgaggcg atccgggagg cggcgtttac acgcggaaat ggcggcaaag
     1261 ccccccgtgc ggagccagaa gaggccggcc cggaatctgc cggcggtgag gggttcaccg
     1321 tcttctggtc gggggaggca gcatccggcc agacgctcga cggccgaatg ctgctgaaac
     1381 ccgccaccca cagcgatccc gcacgggggg tcctgctctc gtttgacgcg aacatgggag
     1441 gaaagagcct catgtgcacc ctcatcctgg acgacgggag cgagaaaggg gtcagggtgc
     1501 aggtatgatc cgggtcgacc gggtccggag ggcggccgcc atcttcctcg gt
//
LOCUS       DAOQVJ010000436         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_111009_length_1545_cov_2.251678, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000436 DAOQVJ010000000
VERSION     DAOQVJ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111009_length_1545_cov_2.251678"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..771)
                     /locus_tag="PKL26_08245"
     CDS             complement(55..771)
                     /locus_tag="PKL26_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS83723.1"
                     /translation="METPEVSAVIPVYNDRESLREAIPRSIEALAAIDPGFELIVAED
                     GSTDGSANEVMVWEQRDARVRLLHSDARLGRGRALTRAFAEARGRIVCYYDVDLATDI
                     SHLPELIGAIRDGADAATGSRLLPESTIQRSGGRELASRGYNLMVRTILGSRLYDHQC
                     GFKAFRKDRLMDLLPSVRDTHWFWDTEVLIRAQRKGYRVVEFPVRWREGSGTTVKKND
                     VYRMGRSILGLWWQLHVEKD"
     gene            complement(773..>1545)
                     /locus_tag="PKL26_08250"
     CDS             complement(773..>1545)
                     /locus_tag="PKL26_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HNS83724.1"
                     /translation="LLASKFGDRKDEISAAWLISRIAIRSNRGLSGEKLGYLKGGFFT
                     LIDALSEQVGKHCVIRLQDPVTSLSHETHEWRVNGQRYDAVISTIPPHTLPGGTLSHL
                     PTIPYQGAACMLLSLDHDVTSGIYWLNMKDPAPYGAVVAHTNFIPLERYGEHLVYLAS
                     YFHGTLPPRKDQAMLEDFCGRFHVQPGDIRWHRMAVDQFAGPLYTTGFRKRVLPYQEK
                     GLYLAGMFSGPNYPERSMEGAVCAGEEVAALLARQEGA"
BASE COUNT          290 a          479 c          468 g          308 t
ORIGIN      
        1 aagagcatga atgcgatgat ccccacggcg atcgcggtag aaatgaagat ggtactaatc
       61 tttctccaca tgcaactgcc accacaaccc aagaattgac cttcccatcc ggtagacgtc
      121 gttctttttc actgtcgtcc cggatccctc tctccaccgg accgggaact cgacgacccg
      181 gtaacccttc cgctgtgccc gtatcagcac ctcggtgtcc cagaaccagt gggtgtcccg
      241 aacagacggg aggagatcca tgaggcggtc ctttcggaac gccttgaacc cgcactggtg
      301 gtcatagagc ctgcttccga gaattgtgcg caccatgagg ttgtagccgc ggctcgcaag
      361 ctcgcggccc ccgctcctct ggattgtgct ctcgggcagg agccgtgagc ctgtagcagc
      421 gtcggcccca tcccgtatgg ccccgatcaa ctcggggagg tgggagatgt ctgtggccaa
      481 atccacgtcg taatagcaga caatacgtcc ccgggcctcg gcaaatgccc tggtgagggc
      541 ccggccccgg cccagccggg catcgctgtg caggagcctt acgcgggcgt cccgctgttc
      601 ccataccatg acctcattcg cgctcccgtc agtgctccca tcctcggcga cgatgagttc
      661 gaaacccggg tcgatggccg ccagcgcctc gattgacctg ggaatggcct cccggaggct
      721 ttccctgtca ttgtacacgg gaatgacggc agagacttcc ggggtctcca tgtcaggccc
      781 cttcctgccg ggcgagcagt gcagcaacct cttccccggc acaaacggcc ccctccatgc
      841 tccgttcggg atagtttggg ccggagaaca tcccggccag gtagaggcct ttctcctggt
      901 agggcaggac ccgcttgcgg aacccagtgg tatagagagg ccccgcgaat tgatcgactg
      961 ccatccggtg ccaccggatg tcccccggct ggacatggaa cctgccgcag aagtcttcga
     1021 gcatcgcctg gtctttcctg ggagggaggg tgccatggaa gtaggacgcc aggtacacga
     1081 ggtgctcccc gtaccgctca aggggaatga agttggtgtg tgcgacgact gctccgtagg
     1141 gggccggatc tttcatgttc agccagtaga tgccggacgt gacgtcatgg tccaatgaga
     1201 ggagcatgca cgcggccccc tggtaaggga tcgtggggag gtgggaaagc gttcccccgg
     1261 ggagggtatg aggggggatt gtcgagatca ctgcatcgta tctctggccg ttcaccctcc
     1321 actcatgggt ctcatgggag agcgacgtta ccggatcctg gagccgtatc acgcaatgct
     1381 tcccgacctg ctcggagagt gcatcgatca gcgtaaagaa gccccctttc aggtatccga
     1441 gcttctctcc tgaaagacca cggttcgagc ggatggcaat ccggctgatg agccatgctg
     1501 ctgaaatctc gtctttccgg tccccgaatt tgctggcgag caggg
//
LOCUS       DAOQVJ010000437         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_111405_length_1541_cov_4.286676, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000437 DAOQVJ010000000
VERSION     DAOQVJ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111405_length_1541_cov_4.286676"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKL26_08255"
     CDS             complement(<1..657)
                     /locus_tag="PKL26_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01210 family radical SAM protein"
                     /protein_id="HNS83725.1"
                     /translation="MNIPVERPLAAWTGSDRIRDQVMSALTIILKTGGCAWNRCRMCS
                     YRHERYGELGQGGLEERLLSQVSWIRNNFCLDEIEAVKIYTSGSLFDTSEVPVRARDA
                     LAEMVRGKIVIAETRPEYVSDDHLSAFLSAIDSGAHDTPLYVAVGVETSNDVVREKCI
                     NKGFSWDEFVKAAARARSSGAGVKAYLLAKPPFLTEREALDDMISSIHDLDGLADMIS
                     L"
     gene            1077..>1541
                     /locus_tag="PKL26_08260"
     CDS             1077..>1541
                     /locus_tag="PKL26_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83726.1"
                     /translation="MTRIRQIIRAKFRVVILMSVFLLLLAGVSAAYELSIYAPMNVQR
                     GMPLVVNGTSNLPAGISVDVILSRSEYTTEEIARETVTLQGPKEFNVVFDTQELSKGQ
                     YKVEVPGVQGYAFLGDSTTIRVVQIIDRTDELTIKSPRTQEMDGSLELRGIIP"
BASE COUNT          351 a          407 c          425 g          358 t
ORIGIN      
        1 gagagagatc atgtctgcaa ggccgtcgag atcatggatc gaggagatca tatcgtcgag
       61 agcctcgcgt tcggtcagga aagggggctt tgcaaggaga taggccttca ccccggcgcc
      121 cgatgagcgg gctctagctg ccgctttcac gaattcatcc caggagaaac ccttgttgat
      181 gcacttctct cgcaccacgt cattgctggt ctcgacaccc actgcgacat acagcggggt
      241 atcatgggct ccgctgtcga ttgcactaag aaatgcggag aggtggtcat cggacacgta
      301 ctccggccgg gtctccgcaa tgactatctt tccccggacc atctctgcaa gagcgtccct
      361 tgcccgcacg ggcacttcac tcgtatcaaa gagactgccg gatgtgtaga tcttcactgc
      421 ctcaatctca tcgaggcaaa aattgttcct gatccaggat acctgggaca acagccgttc
      481 ttctagacct ccttgtccca gttctccata tctttcatgg cggtagctgc acatgcggca
      541 ccggttccag gcgcacccac cggttttgag gatgatagtg agggcagaca tcacctggtc
      601 ccggatgcgg tcgctcccgg tccatgccgc aagcgggcgt tcaacgggta tattcatgga
      661 cacttgctga ttattagagc ccctggctct taagaccctg tatcaacgca aggaggatgc
      721 tctggaaggg tccagccagt gccggtttcc taatcgccgg ttggaaaagg atctttcgcg
      781 tgtttccgtc gaaggcggca attctctttt acgaatgatg ccggggcagt ttccccacat
      841 gagttgttcc tttctcccag gtattaaggc gcgatgcgtg ccaaaggctc atttcgtctt
      901 tctattgcgg ttctcacgaa aattcaggat acaacggggt gggaagacca gaagactatt
      961 caatgctgaa ccggtatatt tacagtacta aatgtgcatg gaatggttca atgtggcatt
     1021 ccagggcata tatcggggtg tttttcccct gcaaagaaga ggccaggtgc aatagaatga
     1081 cacgtatccg acagattatc cgggcaaagt tccgggtggt aattctcatg tcagtattcc
     1141 tgctattgct tgcaggagtg tctgccgcgt atgagctgtc aatctacgcc ccgatgaatg
     1201 tgcagagggg catgccgttg gtggtcaacg ggaccagtaa tctgccggcg gggatatctg
     1261 tagacgtgat actctcccgc tcggaatata ccacagagga aattgccagg gaaacggtga
     1321 ccctccaggg cccaaaagag ttcaacgtgg tgtttgacac ccaggaactc tcgaaaggcc
     1381 agtacaaagt cgaggtaccg ggagtccagg gatatgcgtt tctcggcgat tcgaccacga
     1441 tcagggtggt gcagatcatc gatcgtacgg acgaactgac cataaaatcc ccccgcaccc
     1501 aggagatgga cgggagcctc gaattgcgtg ggataatccc c
//
LOCUS       DAOQVJ010000438         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_112014_length_1536_cov_3.977043, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000438 DAOQVJ010000000
VERSION     DAOQVJ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112014_length_1536_cov_3.977043"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1404)
                     /locus_tag="PKL26_08265"
     CDS             complement(193..1404)
                     /locus_tag="PKL26_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNS83727.1"
                     /translation="MNGETLSLDEALRLVLDHITPLPPEDVSLVESVGRIAASDLYAL
                     VDSPSLDTSRKDGYAVVSGDVAGATQEHPAWLRVLGVMAAGGAHRIAISPGTAVRVLT
                     GARIPDGASGVVSDEYARKGEDHVAIENSAEPGNILPRGSDVSAGTCILRRGQEISPL
                     MAGLLAVAGHSTISVTRSPVVGILGTGDEITAPGQPLLDGKLYASNIITLAGWCRKYG
                     MTMDLAIVSDDLSAIRTTLQDLSTRTDAILTSGGAWKGDRDLVARVLEEMGWRKVFHR
                     IRIGPGKAAGFGMLNGKPVFVLPGGPPSNANAFLQVALPGLLALSGHPNPGLPRMKAT
                     LAAPLQKGDREWTDLFFGTLSFESRMPVFHPQKKRSRLSTIADATAIASIPLGFDHLP
                     EGSIVDVQVLD"
BASE COUNT          298 a          473 c          464 g          301 t
ORIGIN      
        1 gatccatccg gaagagatcc gggcaatcgg gctgcctgcc tgctcctgca gtgccgggag
       61 gggtgcacag atgatcctcg atgtatatcg cgcgaaactg caggactata tcgacgagat
      121 caggagattc atgcctgaaa aagggtgaga gggaggcccg gacacccgca gccgctggcg
      181 gacttgcgtc ctctagtcaa gcacctgcac gtcgacgatc gacccctccg ggaggtggtc
      241 gaaccccaag gggatcgatg cgatcgccgt ggcatctgcg atggtcgaaa ggcggctgcg
      301 ctttttctgg ggatggaaga ccggcatcct gctctcaaac gagagggtgc cgaagaagag
      361 atcggtccac tcacgatcgc ccttttggag cggagcggca agcgttgcct tcatcctggg
      421 cagtccaggg ttcggatgtc cagaaagtgc gaggagaccc ggcaatgcga cctgaaggaa
      481 ggcattcgca ttggagggtg gtccgccggg cagcacaaag actggttttc cgttgagcat
      541 cccaaacccc gcggcttttc ccggcccgat gcggatccgg tggaacacct ttctccaccc
      601 catctcttcg agcacgcggg ccacgaggtc ccggtcaccc ttccaggccc ccccgctggt
      661 gaggatggca tcggtgcgtg tggacaggtc ctgcagagtg gtgcggatgg cagagagatc
      721 atccgataca attgcgagat ccatcgtcat cccgtatttc ctgcaccacc cggcgagagt
      781 gatgatattg cttgcataca gctttccatc caggagaggc tggcccgggg cagtgatctc
      841 atccccggtc ccaagaatcc cgacaacagg gctccgggtg acggaaatgg tgctgtgccc
      901 tgcaacggca aggaggcctg ccatgagggg ggagatctcc tggcctctcc tgaggataca
      961 ggtccctgcc gaaacgtcgc ttcccctggg caggatgttc ccgggttctg cagagttctc
     1021 tatcgccaca tggtcctcac ccttcctcgc atactcatcg gagacgacgc cgcttgcccc
     1081 gtccgggata cgtgccccgg tcaggaccct caccgcggta cctggtgaga tagcaatgcg
     1141 atgtgcgccc ccggccgcca tgactccgag cactctgagc catgcggggt gctcctgcgt
     1201 tgcaccggcc acgtcgcccg atacgactgc gtagccgtct tttcgggatg tgtccagcga
     1261 tggagagtcg acgagggcat aaaggtctga tgcggcgata cgcccgacgc tctcgacaag
     1321 cgaaacgtct tctgggggaa gcggcgtgat gtggtccagc acaagccgca acgcctcatc
     1381 caggctgagc gtttcgccgt tcattcaata ttccctccct cttttctccg atcctgttgc
     1441 acagcagaga ctgctccttt ctcattcata atgcgatgcg atccggttca ccctaccgct
     1501 ggacatccag gacaccaggg atgacccgcg agatct
//
LOCUS       DAOQVJ010000439         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_112630_length_1531_cov_4.429539, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000439 DAOQVJ010000000
VERSION     DAOQVJ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112630_length_1531_cov_4.429539"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKL26_08270"
     CDS             <1..548
                     /locus_tag="PKL26_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83728.1"
                     /translation="LNGIYALVTGQYQAMFTAFLMFGLTLVPYIVAQRMHINLPWFVY
                     LLIALALWIHTAGYIQGYYTLFYPYYDKVAHLISGTAVALLGFLGVIFLDKYWKMTLT
                     TPFIIAFTIIFSVALGAFWEMYEFFVDMVFGGSLAGPMQNGLNDTMFDMIFVLAGSII
                     VAILGHSYLKKNPKEKITGDA"
     gene            complement(677..1528)
                     /locus_tag="PKL26_08275"
     CDS             complement(677..1528)
                     /locus_tag="PKL26_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS83729.1"
                     /translation="MLVVAGAGAGYAYWEGGHPQVTVLSIDGAPEGIVFIADPHLKPG
                     NIGNVRKVIQRINELNPSIVLIGGDFLFGEEEDLSCQEIWSEIEAPVYAVLGNHDYHA
                     GIDATSAIAKSIAVSRTDRDLLTYDMSGLFDETTNLASAAALEEALEAHGVMVQRNEY
                     VMREVSGTPLLLVGVDDGWAGMADPPAVPATDAFSIYMIHEPECRADWDADLILSGHT
                     HGGQFGGTALQFINDAGIMELSGYVRDSRVPVYITRGIGTSNLPWDLRIGATPEIVII
                     NPVSPVQ"
BASE COUNT          293 a          469 c          437 g          332 t
ORIGIN      
        1 ccctcaacgg gatatatgcc cttgtgacgg ggcaatacca ggcgatgttc accgcattcc
       61 tcatgttcgg gctcaccctg gtcccctaca tcgtggccca gaggatgcac atcaacctcc
      121 cgtggtttgt gtacctgctt atcgccctgg cactctggat ccacactgca ggatatatcc
      181 aggggtacta caccctcttc tacccgtatt acgacaaggt ggcccacctg atctcgggga
      241 cggctgtggc gctcctcggg ttcctcggtg tcatcttcct cgacaagtac tggaagatga
      301 ccctcaccac cccgttcatc atcgcattta ccatcatctt ctccgtggca ttgggagcat
      361 tctgggagat gtacgagttc ttcgtggaca tggtctttgg cgggtcgctt gcaggaccga
      421 tgcagaacgg cttaaacgac acgatgttcg acatgatctt cgtccttgca ggttcgatca
      481 tcgtggccat tctcgggcac tcgtacctca agaagaaccc aaaggaaaag atcacggggg
      541 atgcctgaac cgcgtgcggt cgctctcccg tcagaatgat tcctgaagga cggaattgtt
      601 cccgggggac aggatatatc cgcgaaaaat gccctggcat cctttcaccc cggagagcgt
      661 tctccaggat gatctctcac tgcaccgggg ataccgggtt gatgatcacg atctcgggcg
      721 tcgcccctat gcggaggtcc cagggaaggt tcgaagtccc tatccccctc gtgatgtaaa
      781 cagggacgcg actgtcccgg acgtatcccg agagttccat gatcccggcg tcgttgatga
      841 actgaagcgc ggtgccgccg aactgcccgc cgtgggtgtg cccggaaagg atgagatccg
      901 cgtcccagtc ggccctgcac tccggttcgt gaatcatgta gattgaaaac gcatcggtgg
      961 cgggaaccgc gggggggtcc gccatccctg cccacccgtc atcgactccg acgagcagga
     1021 gcggtgtccc cgatacctcg cgcatcacgt actcgttcct ctgcaccatc acgccatgtg
     1081 cttcgagggc ttcctcgagc gctgccgcgg atgcaaggtt ggtggtctcg tcaaagaggc
     1141 cgctcatgtc gtaggtgagg aggtcccggt ccgtgcgcga gactgcgatc gacttggcga
     1201 tggccgaggt ggcgtctatc cctgcgtggt agtcatggtt cccgaggacc gcatagacgg
     1261 gggcctcgat ctcgctccag atctcctggc aggagaggtc ctcctcctcc ccgaagagga
     1321 agtcgccccc tatcaggaca attgaagggt tgagttcatt gatcctctgg atgaccttcc
     1381 tcacattccc aatattccct ggcttgaggt gcgggtcggc gatgaagaca atcccttcag
     1441 gtgcaccgtc aatggagaga accgtgacct gagggtggcc cccctcccag tatgcatagc
     1501 ctgcgcctgc gccggcaact accagcagga c
//
LOCUS       DAOQVJ010000440         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_113660_length_1523_cov_2.867166, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000440 DAOQVJ010000000
VERSION     DAOQVJ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113660_length_1523_cov_2.867166"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKL26_08280"
     CDS             <1..652
                     /locus_tag="PKL26_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83730.1"
                     /translation="GNIMAVRGARPDAGYILLNAHLDTCQSEHDRDLQDSLRYDEARG
                     IFLAESPAGDRQQVAGDDRAGLALILALARGTDLPFKIVCTVREECGQQGIQEIPPEF
                     YADCAFGLTLDRKGRGDIISTYSGRTCAPPHIVMNLIALGKEEGMDYWKVNNGSIADT
                     YHIARFIPAVNISVGYYNPHTRHDYILVDETYGALRLAHASIECPERLLLRPTSSD"
     gene            1133..>1523
                     /locus_tag="PKL26_08285"
     CDS             1133..>1523
                     /locus_tag="PKL26_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83731.1"
                     /translation="MAKHLLAIMEPLIRCSTPSGREEEVAERLLQFVNGRCPGWTAFS
                     DRDGAEGIGNVLAVPTQYVNRSNRFASTKALPIIMAHMDTVDMNDREQLSAYAFSDSI
                     DGHGIVGGDQDFVLGFDDRAGIALILYL"
BASE COUNT          361 a          439 c          423 g          300 t
ORIGIN      
        1 gggaaacatc atggccgtgc ggggtgcgcg gccggatgcc ggttacatac tactaaatgc
       61 ccacctggat acctgccagt ccgagcacga ccgggatctc caggatagcc tgcgctacga
      121 tgaagccagg gggatcttcc tggcagaaag ccctgccggc gatcgccagc aagtggctgg
      181 cgatgatcgc gcgggtttag cactaatact ggctctcgcc cgggggacag atctgccctt
      241 caagatcgta tgcaccgttc gagaagagtg cggtcagcag ggcatccagg aaatacctcc
      301 cgagttctat gccgattgcg ccttcggcct gaccctcgac cggaagggaa ggggggacat
      361 catatcaacc tacagcggaa gaacgtgcgc tccaccccac atcgtcatga acctcatagc
      421 gctgggaaag gaggaaggga tggactattg gaaggtaaac aacggctcga ttgccgatac
      481 ataccatatc gccaggttca tcccggcagt gaacatatcg gtgggctatt acaacccgca
      541 cacccgtcac gactacatcc tggtcgatga gacgtacgga gcgttgcgac ttgcccatgc
      601 aagcatcgag tgccctgagc gcctgcttct ccggccgaca tcatccgact gaacccccgg
      661 gagcccgaac cacccctcac cgggcggatt ggccgggttc tgccgaaccg accgctcgtg
      721 gggggcactc aaccctccca tccgcgtagc gacccccaca ccggagaagg gctccgggag
      781 ccagggcatc tccccgatac gatgccagga gccttacacc gcctgagcac acaataggtt
      841 ttgcacaacg ccccattcat caatccctga tgaatgtacg agacctcttc tgatctccac
      901 ctttgccacg agagaacgtc ccgtgcgata ccaggctgga gcgtaattct ggcccagatc
      961 gtctaaaatc cctcaacaat agccgaattt ttaacccggc caaatgtaaa gagatttatt
     1021 tgaaaacgca tttatcaggc ccagatgaat tgagcatact gagcaactca ggtgatccaa
     1081 tatgagtctt gctgatgtaa attatctgaa aggtcagaag gaataccaga ggatggcgaa
     1141 gcacctgctc gcgatcatgg aaccgctgat acggtgctcc accccgtcgg ggagagagga
     1201 ggaggttgca gaacgcctcc tgcagtttgt caacgggaga tgccccggct ggactgcatt
     1261 ctctgaccgt gatggtgctg aggggattgg aaacgttctt gcagtcccca cccagtatgt
     1321 gaatcggtcg aacaggtttg ccagtacaaa ggccctgccg atcatcatgg cacacatgga
     1381 tacggtcgac atgaatgacc gtgaacaact ctcggcctat gccttctcgg actcgattga
     1441 cggccatggg attgtcgggg gagatcagga tttcgtgctc ggattcgatg acagagccgg
     1501 aattgcactg atcctgtact tgg
//
LOCUS       DAOQVJ010000441         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_114331_length_1517_cov_3.774966, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000441 DAOQVJ010000000
VERSION     DAOQVJ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114331_length_1517_cov_3.774966"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKL26_08290"
     CDS             complement(<1..322)
                     /locus_tag="PKL26_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNS83732.1"
                     /translation="MAAHRVQDMMRLMARRIGRIAESLSDDEVEEFVEEILRARRIYV
                     MGAGRSGLVAKAFAMRLMHLGLSSFVVGETITPAMSVGDLIVVFSGSGRTKTVADIAE
                     TAKEI"
     gene            519..1337
                     /locus_tag="PKL26_08295"
     CDS             519..1337
                     /locus_tag="PKL26_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNS83733.1"
                     /translation="MDPIPYYQDGHFTLYHGDCLEILAEMPEGAFDLVFADPPYNLSN
                     GGFTCQSGRRAPVDKGAWDASNGIEEDFLFHRDWIKACRRVMCEDGSIWISGTYHSIY
                     ACGFALQREGFHVLNDICWFKPNAPPNLSCRYFTASHETLVWARKSRKGTHVFNYQDL
                     KCGDWSEDALKRRGRQMRTVWSIPTSRASEKGNGSHPTQKPLALLTRIVAACTTEGDR
                     VLDPFCGSSTTGLAAWSLGRQFVGIDTEEEYLEMSVRRYLSMKANGNAMRRKAK"
     gene            1334..>1517
                     /locus_tag="PKL26_08300"
     CDS             1334..>1517
                     /locus_tag="PKL26_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS83734.1"
                     /translation="MSPPAPCDFMQSLDEYLRDHIPELVALYRDLHAHPELSGQEART
                     STVIATEMGSAGFRVTR"
BASE COUNT          335 a          448 c          420 g          314 t
ORIGIN      
        1 cgatctcctt ggcagtctcc gcgatatctg ccacggtctt cgtccggccc gaccccgaga
       61 atacgacgat cagatccccc acgctcattg caggggtgat ggtctccccc accacgaagg
      121 aggagagccc gaggtgcatc agccgcatgg caaacgcctt ggccacgagt ccagacctgc
      181 ctgctcccat gacgtagatc cgccgtgcac ggagtatctc ctccacgaac tcctccacct
      241 catcatcaga gagggattcc gcgatccttc cgatcctcct ggccatgagg cgcatcatat
      301 cctgaactct gtgtgccgcc atgcgcttcc cggtgtagat aggcacttca agacgataga
      361 agttgttggt gatagagagg tcctctgctc ccggaccgac catgttttta tctccgccag
      421 gtcacagaat atggtgatac aggcatgttt cggatatgta tacctgtccc tctgcaaaaa
      481 ttccgggaca cgcctataaa ttgacccggg aactgtgaat ggatccaata ccctactacc
      541 aggatggcca tttcaccctg taccatggtg actgcctcga gatacttgcg gagatgccgg
      601 aaggagcgtt cgatctcgtc tttgccgatc caccttataa cctctccaat ggaggcttta
      661 cctgccagtc gggaaggcgg gcaccggtag acaagggtgc ctgggacgca agcaacggca
      721 tcgaggagga cttcttgttc cacagggact ggataaaggc ctgcaggaga gtgatgtgtg
      781 aggatgggag catctggatc agcgggacat accattccat ctatgcctgc ggatttgccc
      841 tgcagagaga agggtttcat gtcttaaacg acatctgctg gttcaagccc aacgccccac
      901 caaacctgag ctgccgttat tttactgcca gccacgaaac tctcgtctgg gcgaggaagt
      961 ccaggaaagg aacacatgtc tttaactacc aggacctgaa gtgcggggac tggagcgagg
     1021 atgcactgaa acgccgtggg cggcagatgc gcactgtctg gagtattccc acttcacgtg
     1081 cctcggagaa aggcaatggg tcccatccca cccagaaacc acttgccctt ctcacacgta
     1141 tcgttgcggc ctgcaccact gaaggggacc gggtgctcga ccctttctgc gggagttcca
     1201 ctaccggcct tgccgcatgg tccctgggga ggcagttcgt ggggattgac acagaggagg
     1261 agtacctgga gatgtctgtc cggcgctacc tctcgatgaa agcgaacgga aatgcaatga
     1321 ggaggaaggc aaaatgagtc caccggcccc ctgcgatttc atgcaatcgc tcgacgaata
     1381 ccttcgcgac cacattccgg aactggtcgc cctgtacagg gaccttcacg cacaccccga
     1441 actttcgggg caggaagccc gcacgtccac tgttatcgcg acggagatgg gctccgcggg
     1501 gttccgcgtt acgcgtg
//
LOCUS       DAOQVJ010000442         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_114689_length_1514_cov_3.976696, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000442 DAOQVJ010000000
VERSION     DAOQVJ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114689_length_1514_cov_3.976696"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKL26_08305"
     CDS             <1..490
                     /locus_tag="PKL26_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS83735.1"
                     /translation="AVLIIGITGALTALHPEYTDLPPDLVSQEIALMNLATIGAILVT
                     SHASLPPVPTIRAAAVAMACGVALTFFSVWGFLVVGWLAGMVMISQLAFLAGASARQG
                     VLMGLFNTASYGGMTLLPFFAGVIAEYAGFPAAFAAIGLCALLVAATIGRCGCKVKAE
                     SP"
     gene            487..>1514
                     /locus_tag="PKL26_08310"
     CDS             487..>1514
                     /locus_tag="PKL26_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNS83736.1"
                     /translation="MTPTPPVRSGTLIPGDRGDGRVVAFFDIGTNSIRLLVVRLYRDC
                     TYQVLTRQKEVVRLGEGEFDEHWINDGAMERAVVVCLRFIDLARSFGAEEFVAVATSA
                     TREAKNKHDLLHRLRQEAHLDVRVISGEEEARLIYLGVAGSTHLGEDRALFIDIGGGS
                     TELCLGGQHSYEVLTSLPLGSIRLTNQFFECDFDGPVSKKRYESICHHIREVLAPAIS
                     RFPRKKPGRVIGSSGTIINLGEIAARTATGPRSAGPWILDRKGLSRVIPLLCSLPLKE
                     RRNVPGINPERADIIIAGAAVLDCLMEAFSFDRIEITGRGLQDGLLADYLTRMEDFPL
                     LGQLNVRER"
BASE COUNT          268 a          487 c          466 g          293 t
ORIGIN      
        1 cgcggtcctg atcatcggta tcaccggggc cctcactgcg ctccaccccg agtacacgga
       61 cctgccgccg gacctcgtct cccaggagat cgcactgatg aacctcgcga ccatcggcgc
      121 gatcctggtc acctcccatg caagcctccc tcccgttccc accatcaggg ctgcagcggt
      181 ggcaatggcc tgcggtgtgg cgctgacctt cttctcggta tggggattcc tcgtcgtcgg
      241 gtggcttgca gggatggtga tgatctccca gctcgcgttc ctggcaggcg ccagcgcccg
      301 ccagggggta ttgatggggc ttttcaacac cgccagttac ggcggcatga cgctcctccc
      361 cttctttgcg ggagttatcg cagaatacgc aggatttcct gcggcatttg ctgccatcgg
      421 cctgtgcgca ctcctcgttg ccgcaaccat agggcgctgc ggctgcaaag tgaaggcaga
      481 gagtccatga cccccacccc gcccgtgcgc tcaggaacac tcattcccgg cgaccgcggg
      541 gacggccggg tggtcgcatt cttcgatatc ggcaccaact cgatccgcct cctcgtggtc
      601 cggctctaca gggactgcac ctaccaggtc ctgacccgcc agaaggaggt cgtgaggctt
      661 ggggaagggg aattcgacga gcattggatc aatgacgggg caatggagcg ggccgtggtg
      721 gtctgtctga ggttcatcga tctcgcacgc tcctttggtg cggaggagtt cgtggcagtg
      781 gcgacatctg ccacgcggga ggcaaagaac aagcacgacc tcctccaccg gctcaggcag
      841 gaggcgcacc tcgatgtccg tgtcatctcc ggtgaggagg aagcgcggct catctacctc
      901 ggcgttgcag gcagcaccca tctcggggag gaccgcgcgc ttttcatcga tatcggcggg
      961 gggagcactg aactctgcct tggggggcag cactcgtacg aggtactcac ctcgctcccg
     1021 ctcgggtcca tccggcttac aaaccagttc ttcgaatgcg actttgacgg gcccgtctcg
     1081 aagaaacggt acgaatctat ctgccaccac atccgggagg tgctcgcacc ggcaatctcc
     1141 aggttcccca ggaagaagcc gggacgcgtc atcgggagtt ccggcactat cataaacctc
     1201 ggggagattg cagcccggac cgcaaccggt ccgagatccg cggggccctg gatcctggat
     1261 cgaaagggcc tctcgcgcgt gatcccgctc ctctgttccc tccctctcaa ggagaggcgg
     1321 aacgtgccgg gtatcaaccc tgaacgggcc gacatcatca tcgccggggc tgcagtcctg
     1381 gactgcctga tggaggcgtt ctcgttcgat aggatagaga tcacggggag aggtcttcag
     1441 gacgggctcc ttgcagacta cctcaccagg atggaagatt ttccgctcct tggccagctc
     1501 aacgtccgtg aacg
//
LOCUS       DAOQVJ010000443         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_116481_length_1500_cov_5.974394, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000443 DAOQVJ010000000
VERSION     DAOQVJ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116481_length_1500_cov_5.974394"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..420)
                     /locus_tag="PKL26_08315"
     CDS             complement(103..420)
                     /locus_tag="PKL26_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HNS83737.1"
                     /translation="MQEYPVKRGYTKDLQTTMGAKLKDCFNVEPKKVGDHYQITYGAL
                     KVLDVSTGKEKKTLVVDTKSDTTASDEVILETNRRFRKYLDEVTGYSTKDRVKKAKSV
                     EPE"
     gene            438..1184
                     /locus_tag="PKL26_08320"
     CDS             438..1184
                     /locus_tag="PKL26_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HNS83738.1"
                     /translation="MEYVKLMSPDPTTDIKIFSRPLSGPGSSVLMGFPGSGLVGSIAL
                     QYMVDQLEFEQIGSINSKYFPALAMMNKGVINVPVRVYEKGNISVIVADIPIHPMICY
                     EVANGVMDWLEPFSIKEVVTIAGIITNEPEKRVFGVASNEEALSRIQDSTIILPVGSI
                     SGIASSILTECKIRNIPAYGLLGETINAPDPRASAAAIEVLNRMYDIQLDVKPLIEQA
                     EEIESTMQKLAEQVKTAETMPKKEQLPMYG"
     gene            1190..>1500
                     /locus_tag="PKL26_08325"
     CDS             1190..>1500
                     /locus_tag="PKL26_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HNS83739.1"
                     /translation="MKCFALTGIAPQVIRELQEGKARTLELQSTHNVVTLAGIPAGSF
                     VFMTSVDMEDISPGDSGIIVELLAVSITMKRIMEYSHGLHYEERERMSARIKVKCVGS
                     ST"
BASE COUNT          350 a          388 c          412 g          350 t
ORIGIN      
        1 gaatgtgcca ttccggtaca tatcgtggat gcgggggagt acgggcaccc cagggctgct
       61 gccgccctcc ccctgaagcc tgcgatcgtg atggagtgaa gcctactcgg gctccacgct
      121 tttcgccttt ttcacacgat ctttcgtgga ataccctgtc acctcatcga ggtattttcg
      181 aaagcgccgg ttggtttcga gaatgacctc gtcggatgca gtggtatcag atttggtatc
      241 cactacaagg gtctttttct ccttcccggt tgagacgtcc aggactttca gggccccgta
      301 cgtgatctgg taatgatcac ccaccttttt tggttcaaca ttaaagcagt ccttgagctt
      361 ggcccccatg gttgtctgga gatccttcgt ataccctcgt ttcacaggat attcttgcat
      421 aatacttttt taggtggatg gaatatgtta agctaatgtc gcctgatcca accacagata
      481 tcaagatctt ctcgcgacca ctgtccggtc ccggatcatc tgtccttatg ggatttccgg
      541 ggagcggcct tgtggggagc atcgcgctcc agtacatggt ggaccagctg gagttcgagc
      601 agatcgggag tatcaacagc aaatatttcc ctgcccttgc catgatgaac aagggagtga
      661 tcaatgtccc ggtgagggtg tatgagaagg ggaatatctc tgtgatcgtc gcggacatcc
      721 ccatccaccc catgatctgc tacgaggtgg cgaacggggt gatggactgg ctggaaccat
      781 tctccatcaa ggaggtcgtc acgatcgcgg gcatcatcac caacgaaccc gagaagagag
      841 tgtttggagt tgcaagcaac gaagaggcac tctccaggat acaggactcc actatcatcc
      901 tcccagtcgg aagcatttca gggattgcaa gcagcatcct caccgagtgt aagatcagga
      961 atatccctgc gtatggtctt ctcggagaga cgatcaatgc tcccgatcca cgggcatcag
     1021 ctgccgccat agaggtcctg aacaggatgt atgacattca actcgatgtc aagccgctta
     1081 tcgagcaggc ggaagagatc gagtcaacca tgcagaagct ggcagaacag gtgaagaccg
     1141 cagagacgat gccgaagaaa gaacagcttc ccatgtacgg gtgatcttca tgaagtgctt
     1201 cgcgttaacc ggaatcgccc cacaggtgat cagagagctc caggagggca aggccaggac
     1261 cctggaacta cagagtacgc acaatgtggt gacccttgca ggtattcctg ccggatcgtt
     1321 cgtattcatg acttccgtcg atatggagga tatctccccg ggagattcag gaatcatcgt
     1381 cgagctcctc gcggtgagca tcacgatgaa gcggatcatg gagtattcgc acggtctgca
     1441 ttacgaggaa cgggagcgga tgtctgcccg aatcaaggta aaatgcgtgg gttcctcgac
//
LOCUS       DAOQVJ010000444         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1091
            NODE_116506_length_1500_cov_3.279585, whole genome shotgun
            sequence.
ACCESSION   DAOQVJ010000444 DAOQVJ010000000
VERSION     DAOQVJ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852434
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:26:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,667
            CDSs (total)                      :: 1,642
            Genes (coding)                    :: 1,632
            CDSs (with protein)               :: 1,632
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 4 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116506_length_1500_cov_3.279585"
                     /isolate="AMR_MDS_1091"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKL26_08330"
     CDS             <1..502
                     /locus_tag="PKL26_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNS83740.1"
                     /translation="ILVIINVILTMIVDLVFASAILSTLSETIGQMGMGEIFLIETIG
                     MVVLAIILIILMLVLLFIVAGWLHLFVYLLGGRKGYAETVKALIFGSTPYMLIGWIPI
                     IGLFIGGIWSLILGILGIRELHQVSTGRAAGAVVLSAFILFIVIVLVAAWFIISLVSV
                     EPVLVT"
     gene            1137..>1500
                     /locus_tag="PKL26_08335"
     CDS             1137..>1500
                     /locus_tag="PKL26_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNS83741.1"
                     /translation="MSSGKQEIRARAREVRAAIPAEQRVALSRAIREQVLALLDGTDP
                     VLVYASKPPEVDTLPLISTMISRGTRVVVPIIEKEERTLRLSYLDDAALLSVSTFSVP
                     EPIGHEVPADPGEIPVVIV"
BASE COUNT          280 a          430 c          455 g          335 t
ORIGIN      
        1 cattcttgtc atcatcaatg ttattctcac catgatcgtg gacctcgtgt tcgcgagcgc
       61 aatactctca acgctctcgg agaccattgg ccagatgggc atgggggaga tcttcctgat
      121 tgagacgatc gggatggtgg tgctcgcaat catactgatc atactgatgc tggtcctcct
      181 cttcatcgtc gcaggatggc tgcacctctt cgtgtatctc cttggagggc ggaagggcta
      241 tgcagagacc gtaaaagccc tgatatttgg gtccactccc tacatgctga tcgggtggat
      301 cccaatcatc gggctcttca tcggggggat atggtcgctg atcctcggga ttctcgggat
      361 ccgggaactg caccaggtct cgacagggcg ggcagcaggg gcggtggtcc tttccgcgtt
      421 catccttttc attgtcatcg tgctcgtggc tgcctggttc atcatctcgc ttgtcagcgt
      481 cgagcccgtg ctcgtcacct gaaccccccc ttttttcctg atcggtgggg catgaactcc
      541 aggtgcggca ggccgtgagg ggatcagtgc gcagaggaga tcccgaagag aggccggtta
      601 ccctggccgg cgtcccattt tactttgaaa atggctgcac cattttcatg aatgtgtatg
      661 cgtgtgtctc gaagagaatc atgtggtgtt atcggacggg caactgacct ccccggtgct
      721 tccgtccctc tccactcatt gcggtgcaaa cgccgcggga ggcatgtgtc gcaacacctg
      781 gcagacgaat tgcaatgcgg ggacgaatct ggtgatcccc cgtcacgaaa agaaggttcc
      841 aggccgggaa tgcctggagg tgggggatgt atgcagtgtt catgaggcga ttcatctctt
      901 ccgggccggc gtccctcgct gccccgggtg ctggtcgagc cgggcaaccc ccgaccccgg
      961 ctgcaggaga gtggttggcg ggatgtagta ataaactttg ttaattacgt agcacttaat
     1021 aagtaaagtt ttgtaaagtc ggaaacactc ctcttccctc gctgtcgcaa cccgcttccc
     1081 ggtgacggac cgcaccctta attcttcttc cgggacgaat ggtacagtac agcagaatga
     1141 gcagcgggaa gcaggagata cgcgccaggg cgcgggaggt cagggctgcc atcccggcgg
     1201 agcagagggt cgcgctctca cgggccatca gggagcaggt gctcgcgctc ctggacggca
     1261 cggaccccgt gctcgtctac gcgtcgaagc ctcccgaagt ggatacgctc ccgctcatct
     1321 cgaccatgat atcgcggggg acacgggtgg tggtccccat catcgaaaag gaggagagaa
     1381 cgctacggct ctcttatctc gacgatgcag ccctcctctc ggtgagcacg ttctcggtcc
     1441 ccgagcccat cgggcacgag gtgccagccg accccggcga gatcccggtg gtgatcgtcc
//
